--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/rfam.pl	Thu Jul 31 03:08:35 2014 -0400
@@ -0,0 +1,103 @@
+#!/usr/bin/perl -w
+#Filename:
+#Author: Tian Dongmei
+#Email: tiandm@big.ac.cn
+#Date: 2013/7/19
+#Modified:
+#Description: 
+my $version=1.00;
+
+use strict;
+use Getopt::Long;
+use File::Basename;
+
+my %opts;
+GetOptions(\%opts,"i=s","ref=s","index:s","v:i","p:i","o=s","time:s","h");
+if (!(defined $opts{i} and defined $opts{o} ) || defined $opts{h}) { #necessary arguments
+&usage;
+}
+
+my $filein=$opts{'i'};
+my $dir=$opts{'o'};
+unless ($dir=~/\/$/) {$dir.="/";}
+my $rfam=$opts{'ref'};
+my $mis=defined $opts{'v'}? $opts{'v'} : 0;
+my $index=defined $opts{'index'} ? $opts{'index'} : "";
+my $threads=defined $opts{'p'} ? $opts{'p'} : 1;
+
+if (not -d $dir) {
+	mkdir $dir;
+}
+
+
+my $time=Time();
+if (defined $opts{'time'}) {
+	$time=$opts{'time'};
+}
+my $mapdir=$dir."/rfam_match_".$time;
+if(not -d $mapdir){
+	mkdir $mapdir;
+}
+chdir $mapdir;
+###check genome index
+if (-s $index.".1.ebwt") {
+}else{
+	&checkACGT($rfam);
+	`bowtie-build $rfam $rfam`;
+	$index="$rfam";
+}
+### genome mapping
+`bowtie -v $mis -f -p $threads -k 1 $index $filein --al rfam_mapped.fa --un rfam_not_mapped.fa > rfam_mapped.bwt`;
+
+sub checkACGT{
+	my $string;
+	open IN,"<$rfam";
+	while (my $aline=<IN>) {
+		if ($aline!~/^>/) {
+			$aline=~s/U/T/gi;
+		}
+		$string .=$aline;	
+	}
+	close IN;
+	$rfam=basename($rfam);
+	open OUT, ">$rfam";
+	print OUT $string;
+	close OUT;
+}
+
+sub Time{
+        my $time=time();
+        my ($sec,$min,$hour,$day,$month,$year) = (localtime($time))[0,1,2,3,4,5,6];
+        $month++;
+        $year+=1900;
+        if (length($sec) == 1) {$sec = "0"."$sec";}
+        if (length($min) == 1) {$min = "0"."$min";}
+        if (length($hour) == 1) {$hour = "0"."$hour";}
+        if (length($day) == 1) {$day = "0"."$day";}
+        if (length($month) == 1) {$month = "0"."$month";}
+        #print "$year-$month-$day $hour:$min:$sec\n";
+        return("$year-$month-$day-$hour-$min-$sec");
+}
+sub usage{
+print <<"USAGE";
+Version $version
+Usage:
+$0 -i -o
+options:
+-i input file# input reads fasta/fastq file
+-ref input file# rfam file, which do not contain miRNAs
+-index file-prefix #(must be indexed by bowtie-build) The parameter
+                string must be the prefix of the bowtie index. For instance, if
+                the first indexed file is called 'h_sapiens_37_asm.1.ebwt' then
+                the prefix is 'h_sapiens_37_asm'.##can be null
+-v <int>           report end-to-end hits w/ <=v mismatches; ignore qualities,default 0;
+
+-p/--threads <int> number of alignment threads to launch (default: 1)
+
+-o output directory 
+-time sting #make directory time,default is the local time
+-h help
+USAGE
+exit(1);
+}
+