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1 #!/usr/bin/perl -w
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2 #Filename:
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3 #Author: Chen Tingting
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4 #Email: chentt@big.ac.cn
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5 #Date: 2014/5/13
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6 #Modified:
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7 #Description: cluster annotate
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8 my $version=1.00;
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9
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10 use strict;
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11 use Getopt::Long;
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12
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13 my %opts;
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14 GetOptions(\%opts,"i=s","g=s","n=s","r=s","p=s","o=s","t=s","l=s","h");
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15 if (!(defined $opts{i} and defined $opts{g} and defined $opts{n} and defined $opts{r} and defined $opts{p} and defined $opts{o} and defined $opts{t} and defined $opts{l}) || defined $opts{h}) { #necessary arguments
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16 &usage;
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17 }
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18
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19 #my %gene;
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20 my %gene1;
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21 open IN,"<$opts{g}";
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22 open OUT ,">$opts{l}";
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23 print OUT "#ID\tchr\tstart\tend\tstrand\n";
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24 my $n=1;
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25 while (my $aline=<IN>) {
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26 chomp $aline;
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27 next if($aline=~/^\#/);
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28 my @tmp=split/\t/,$aline;
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29 my $ID;
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30 if ($tmp[2] eq "gene") {
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31 $tmp[0]=~s/Chr\./Chr/;
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32 #$tmp[0]=~s/Chr/chr/;
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33 my @infor=split/;/,$tmp[8];
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34 for (my $i=0;$i<@infor ;$i++) {
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35 if ($infor[$i]=~/Alias\=(\S+)$/) {
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36 $ID=$1;
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37 last;
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38 }
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39 else {
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40 $ID="unknown$n";
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41 $n++;
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42 }
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43 }
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44 #$gene{$tmp[0]}{$ID}=[$tmp[3],$tmp[4],$tmp[6]];#$gene{chr}{geneID}=[start,end,strand]
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45 push @{$gene1{$ID}},[$tmp[3],$tmp[4],$tmp[0]];
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46 print OUT "$ID\t$tmp[0]\t$tmp[3]\t$tmp[4]\t$tmp[6]\n";
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47 }
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48 }
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49 #while (my $aline=<IN>) {
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50 # chomp $aline;
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51 # next if($aline=~/^\#/);
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52 # my @tmp=split/\t/,$aline;#ID chr start end strand
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53 # push @{$gene1{$tmp[0]}},[$tmp[2],$tmp[3],$tmp[1]];
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54 # #$gene{$tmp[1]}{$tmp[0]}=[$tmp[2],$tmp[3],$tmp[1]];
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55 #}
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56 close IN;
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57 close OUT;
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58
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59 my %nat;
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60 open TMP,">$opts{t}";
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61 print TMP "#NAT_ID\tGene\tStrand\tChr\tGene_start\tGene_end\tAntiGene\tStrand\tChr\tAntiGene_start\tAntiGene_end\tType1\tType2\tNATS1_start\tNATS1_end\tNATS2_start\tNATS2_end\n";
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62
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63 open IN,"<$opts{n}";
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64 my $nat_n=1;
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65 while (my $aline=<IN>) {
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66 next if($aline=~/^\#/);#osj LOC_Os05g02659 - LOC_Os01g24200 + trans 1559 1802 660 905 246 100nt -
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67 chomp $aline;
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68 my @arr=split /\t/,$aline;
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69 my ($ns,$ne,$ns2,$ne2)=(0,0,0,0);
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70 if ($arr[11]=~/Nearby/) {
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71 ($ns,$ne)=&nearby($gene1{$arr[1]}[0][0],$gene1{$arr[1]}[0][1],$gene1{$arr[3]}[0][0],$gene1{$arr[3]}[0][1]);
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72 push @{$nat{$gene1{$arr[1]}[0][2]}},[$ns,$ne,$arr[5],$arr[11],"NATs".$nat_n];
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73 print TMP "NATs$nat_n\t$arr[1]\t$arr[2]\t$gene1{$arr[1]}[0][2]\t$gene1{$arr[1]}[0][0]\t$gene1{$arr[1]}[0][1]\t$arr[3]\t$arr[4]\t$gene1{$arr[3]}[0][2]\t$gene1{$arr[3]}[0][0]\t$gene1{$arr[3]}[0][1]\t$arr[5]\t$arr[11]\t$ns\t$ne\t$ns\t$ne\n";
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74 $nat_n++;
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75 }else{
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76 $ns=$gene1{$arr[1]}[0][0]+$arr[6]-1;
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77 $ne=$gene1{$arr[1]}[0][0]+$arr[7]-1;
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78 $ns2=$gene1{$arr[3]}[0][1]-$arr[9]+1;
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79 $ne2=$gene1{$arr[3]}[0][1]-$arr[8]+1;
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80 push @{$nat{$gene1{$arr[1]}[0][2]}},[$ns,$ne,$arr[5],$arr[11],"NATs$nat_n"."_1"];#start,end,class1,class2
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81 push @{$nat{$gene1{$arr[3]}[0][2]}},[$ns2,$ne2,$arr[5],$arr[11],"NATs$nat_n"."_2"];
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82 print TMP "NATs$nat_n\t$arr[1]\t$arr[2]\t$gene1{$arr[1]}[0][2]\t$gene1{$arr[1]}[0][0]\t$gene1{$arr[1]}[0][1]\t$arr[3]\t$arr[4]\t$gene1{$arr[3]}[0][2]\t$gene1{$arr[3]}[0][0]\t$gene1{$arr[3]}[0][1]\t$arr[5]\t$arr[11]\t$ns\t$ne\t$ns2\t$ne2\n";
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83 $nat_n++;
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84 }
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85 }
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86 close IN;
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87 close TMP;
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88
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89 my %repeat;
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90 open IN,"<$opts{'r'}";
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91 my $first=<IN>;
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92 $first=<IN>;
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93 $first=<IN>;
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94 while (my $aline=<IN>) {
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95 chomp $aline;
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96 $aline=~s/^\s+//;
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97 my @tmp=split/\s+/,$aline;
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98 $tmp[4]=~s/chr0/Chr/;
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99 $tmp[4]=~s/chr/Chr/;
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100 push @{$repeat{$tmp[4]}},[$tmp[5],$tmp[6],$tmp[10]];#start,end,class
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101 #print "$tmp[4]\t$tmp[5]\t$tmp[6]\t$tmp[10]\n";
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102 }
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103 close IN;
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104
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105 my %phase;
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106 open IN,"<$opts{'p'}";
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107 while (my $aline=<IN>) {
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108 chomp $aline;
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109 next if($aline=~/^\#/);
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110 my @tmp=split/\t/,$aline;
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111 if ($tmp[5]>=25) {
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112 $phase{$tmp[0]}=$tmp[5];
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113 }
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114 }
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115 close IN;
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116
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117 my $filein=$opts{'i'};
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118 my $fileout=$opts{'o'};
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119 open IN,"<$filein"; #input file
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120 open OUT,">$fileout"; #output file
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121 while (my $aline=<IN>) {
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122 chomp $aline;
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123 if($aline=~/^\#/){
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124 print OUT "$aline\tPhase\tLong\tRepeatClass\tNatClass1\tNatClass2\tNatID\n";
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125 next;
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126 }
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127 my @tmp=split/\t/,$aline;
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128 my @inf=split/\:/,$tmp[0];
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129 my @pos=split/\-/,$inf[1];
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130 my $chr=$inf[0];
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131 $chr=~s/Chr0/Chr/;
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132 my $start=$pos[0];
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133 my $end=$pos[1];
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134 #=========Repeat============
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135 my @repeat;
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136 if (defined(@{$repeat{$chr}})) {
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137 my @r_array=sort {$a->[0] <=> $b->[0]} @{$repeat{$chr}};
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138 for (my $i=0;$i<@r_array ;$i++) {
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139 if ($start<$r_array[$i][0] && $end>$r_array[$i][0]) {
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140 push @repeat,$r_array[$i][2];
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141 }
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142 elsif($start>$r_array[$i][0] && $start<$r_array[$i][1]){
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143 push @repeat,$r_array[$i][2];
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144
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145 }
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146 elsif($end<$r_array[$i][0]){
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147 last;
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148 }
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149 else{next;}
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150 }
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151 }
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152 my $repeat;
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153 if (@repeat==0) {
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154 $repeat="\/";
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155 }
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156 else{
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157 $repeat=join ";",@repeat;
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158 }
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159 #=========nat===============
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160 my @nat1;#class 1
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161 my @nat2;#class 2
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162 my @nat;#nat ID
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163 #foreach my $chr (keys %nat) {
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164 my @n_array=sort {$a->[0] <=> $b->[0] } @{$nat{$chr}};
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165 for (my $i=0;$i<@n_array ;$i++) {
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166 if ($start<$n_array[$i][0] && $end>$n_array[$i][0]) {
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167 push @nat1,$n_array[$i][2];
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168 push @nat2,$n_array[$i][3];
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169 push @nat,$n_array[$i][4];
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170 }
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171 elsif($start>$n_array[$i][0] && $start<$n_array[$i][1]){
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172 push @nat1,$n_array[$i][2];
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173 push @nat2,$n_array[$i][3];
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174 push @nat,$n_array[$i][4];
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175 }
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176 elsif($end<$n_array[$i][0]){
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177 last;
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178 }
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179 else{next;}
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180 }
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181 #}
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182
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183 my $nat1;
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184 my $nat2;
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185 my $nat;
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186 if (@nat1==0) {
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187 $nat1="\/";
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188 }
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189 else{
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190 $nat1=join ";",@nat1;
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191 }
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192 if (@nat2==0) {
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193 $nat2="\/";
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194 }
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195 else{
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196 $nat2=join ";",@nat2;
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197 }
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198 if (@nat==0) {
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199 $nat="\/";
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200 }
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201 else{
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202 $nat=join ";",@nat;
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203 }
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204 #========phase==============
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205 my $phase="\/";
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206 if (defined($phase{$tmp[0]})) {
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207 $phase="phase";
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208 }
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209 #=========long===============
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210 my $long="\/";
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211 if ($tmp[1] eq "\>30nt" and $tmp[2]>=0.5) {
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212 $long="long";
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213 }
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214 print OUT "$aline\t$phase\t$long\t$repeat\t$nat1\t$nat2\t$nat\n";
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215 }
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216
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217 close IN;
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218 close OUT;
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219
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220 sub nearby{
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221 my @p=@_;
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222 my ($s,$e)=(0,0);
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223 if ($p[1]<$p[2]) {
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224 $s=$p[1];
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225 $e=$p[2];
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226 }else{
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227 $s=$p[3];
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228 $e=$p[0];
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229 }
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230 return ($s,$e);
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231 }
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232
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233 sub usage{
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234 print <<"USAGE";
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235 Version $version
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236 Usage:
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237 $0 -i -o -g -n -r -p -t -l
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238 options:
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239 -i input file
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240 -g gff file
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241 -n NATs file
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242 -r repeat file
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243 -p phase file
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244 -o output file
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245 -t nat output file
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246 -l genelist output file
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247 -h help
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248 USAGE
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249 exit(1);
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250 }
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251
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