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1 #!/usr/bin/perl -w
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2 #Filename:
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3 #Author: Tian Dongmei
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4 #Email: tiandm@big.ac.cn
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5 #Date: 2013/7/19
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6 #Modified:
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7 #Description:
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8 my $version=1.00;
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9
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10 use strict;
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11 use Getopt::Long;
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12
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13 my %opts;
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14 GetOptions(\%opts,"i=s","g=s","index:s","v:i","p:i","r:s","o=s","h");
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15 if (!(defined $opts{i} and defined $opts{o} ) || defined $opts{h}) { #necessary arguments
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16 &usage;
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17 }
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18
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19 my $filein=$opts{'i'};
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20 my $fileout=$opts{'o'};
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21 unless ($fileout=~/\/$/) {$fileout.="/";}
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22 my $genome=$opts{'g'};
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23 my $mis=defined $opts{'v'}? $opts{'v'} : 0;
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24 my $hits=defined $opts{'r'}? $opts{'r'} : 25;
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25 my $index=defined $opts{'index'} ? $opts{'index'} : "";
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26 my $threads=defined $opts{'p'} ? $opts{'p'} : 1;
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27
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28
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29 #my $time=time();
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30 #my $mapdir=$fileout."/genome_match_".$time;
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31 my $mapdir=$fileout."/genome_match";
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32 mkdir $mapdir;
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33 chdir $mapdir;
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34 ###check genome index
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35 if (-s $index.".1.ebwt") {
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36 }else{
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37 `bowtie-build $genome genome`;
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38 $index="genome";
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39 }
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40
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41 ### genome mapping
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42 `bowtie -v $mis -f -p $threads -m $hits -a --best --strata $index $filein --al genome_mapped.fa --un genome_not_mapped.fa > genome_mapped.bwt`;
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43
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44 #`convert_bowtie_to_blast.pl genome_mapped.bwt genome_mapped.fa $genome > genome_mapped.bst`;
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45
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46 sub usage{
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47 print <<"USAGE";
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48 Version $version
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49 Usage:
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50 $0 -i -o
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51 options:
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52 -i input file# input reads fasta/fastq file
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53 -g input file# genome file
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54 -index file-prefix #(must be indexed by bowtie-build) The parameter
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55 string must be the prefix of the bowtie index. For instance, if
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56 the first indexed file is called 'h_sapiens_37_asm.1.ebwt' then
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57 the prefix is 'h_sapiens_37_asm'.##can be null
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58 -v <int> report end-to-end hits w/ <=v mismatches; ignore qualities,default 0;
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59
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60 -p/--threads <int> number of alignment threads to launch (default: 1)
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61
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62 -r int a read is allowed to map up to this number of positions in the genome
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63 default is 25
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64
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65 -o output directory
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66
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67 -h help
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68 USAGE
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69 exit(1);
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70 }
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71
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