Mercurial > repos > big-tiandm > sirna_plant
diff SampleDEGseqMerge.pl @ 21:9dcffd531c76 draft
Uploaded
author | big-tiandm |
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date | Wed, 05 Nov 2014 21:09:35 -0500 |
parents | 07745c0958dd |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/SampleDEGseqMerge.pl Wed Nov 05 21:09:35 2014 -0500 @@ -0,0 +1,94 @@ +#!/usr/bin/perl -w +#Filename: +#Author: Tian Dongmei +#Email: chentt@big.ac.cn +#Date: 2014-05-21 +#Modified: +#Description: merged deg file and total information +my $version=1.00; + +use strict; +use Getopt::Long; + +my %opts; +GetOptions(\%opts,"i:s@","mark:s@","f:s","o=s","n=s","h"); +if (!(defined $opts{o} ) || defined $opts{h}) { #necessary arguments +&usage; +} + +my @filein=@{$opts{'i'}}; +my @mark=@{$opts{'mark'}}; +my $fileout=$opts{'o'}; +my $number=$opts{'n'}; + +my %hash; +open IN,"<$filein[0]"; #input file + +while (my $aline=<IN>) { + chomp $aline; + next if($aline=~/^\"/); + my @temp=split/\t/,$aline; + $hash{$temp[0]}=$temp[4]."\t".$temp[6]."\t".$temp[7]."\t".$temp[-1]; +} +close IN; + +for (my $i=1;$i<=$#filein;$i++) { + open IN,"<$filein[$i]"; #input file + + while (my $aline=<IN>) { + chomp $aline; + next if($aline=~/^\"/); + my @temp=split/\t/,$aline; + if (!(defined $hash{$temp[0]})) { + print "Not find $temp[0]in sample one!\n"; + next; + } + $hash{$temp[0]} .="\t".$temp[4]."\t".$temp[6]."\t".$temp[7]."\t".$temp[-1]; + } + close IN; +} + +open OUT,">$fileout"; #output file +my $deg_title; +foreach (@mark) { + $deg_title.="log2(Fold_change)\tp_value\tq_value\t".$_."\t"; +} +$deg_title=~s/\s+$//; +my %function; +my $title; +open F,"<$opts{f}"; +while (my $aline=<F>) { + chomp $aline; + if($aline=~/^\#/){ + my $title=$aline; + my @title=split/\t/,$aline; + $title[2+$number].="\t".$deg_title; + $title=join"\t",@title; + print OUT "$title\n"; + next; + } + my @temp=split/\t/,$aline; + $temp[2+$number].="\t".$hash{$temp[0]}; + my $temp=join"\t",@temp; + print OUT "$temp\n"; + +} +close F; +close OUT; + +sub usage{ +print <<"USAGE"; +Version $version +Usage: +$0 -i -o -mark -f +options: +-i input file # -i output_score.txt -i output_score.txt -i output_score.txt +-mark sample name # -mark sam1_VS_sam2 -mark sam1_VS_sam3 -mark sam2_VS_sam3 +-f cluster file +-n sample number +-o output file +-h help +USAGE +exit(1); +} +