# HG changeset patch # User Bjoern Gruening # Date 1331802568 -3600 # Node ID d5d5999ff4f1e3ffe934ddcccd0c92bc5dc18298 # Parent 5663d1a1b29c0e25ea17754fff964dd674a500a5 change directory structure diff -r 5663d1a1b29c -r d5d5999ff4f1 augustus.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/augustus.xml Thu Mar 15 10:09:28 2012 +0100 @@ -0,0 +1,186 @@ + + gen prediction for eukaryotic genomes + augustus + --strand=$strand + $noInFrameStop + $gff + $protein + $introns + $start + $stop + $cds + $codingseq + $singlestrand + $input_genome + --genemodel=$genemodel + --species=$organism + --outfile=$output + + #please set export AUGUSTUS_CONFIG_PATH=/path_to_augustus/augustus/config + #or use the --AUGUSTUS_CONFIG_PATH=path switch + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + augustus + + + +**What it does** + +AUGUSTUS is a gene prediction program for eukaryotes written by Mario Stanke and Oliver Keller. +It can be used as an ab initio program, which means it bases its prediction purely on the +sequence. AUGUSTUS may also incorporate hints on the gene structure coming from extrinsic sources +such as EST, MS/MS, protein alignments and synthenic genomic alignments. + +----- + +**Parameters** + +Gene Model:: + + partial : allow prediction of incomplete genes at the sequence boundaries (default) + intronless : only predict single-exon genes like in prokaryotes and some eukaryotes + complete : only predict complete genes + atleastone : predict at least one complete gene + exactlyone : predict exactly one complete gene + + + +**Example** + +Suppose you have the following DNA formatted sequences:: + + >Seq1 + cccgcggagcgggtaccacatcgctgcgcgatgtgcgagcgaacacccgggctgcgcccg + ggtgttgcgctcccgctccgcgggagcgctggcgggacgctgcgcgtcccgctcaccaag + cccgcttcgcgggcttggtgacgctccgtccgctgcgcttccggagttgcggggcttcgc + cccgctaaccctgggcctcgcttcgctccgccttgggcctgcggcgggtccgctgcgctc + ccccgcctcaagggcccttccggctgcgcctccaggacccaaccgcttgcgcgggcctgg + +Running this tool will produce this:: + + # ----- prediction on sequence number 1 (length = 1992969, name = scaffold1|size1992969) ----- + # + # Constraints/Hints: + # (none) + # Predicted genes for sequence number 1 on both strands + # start gene g1 + scaffold1|size1992969 AUGUSTUS gene 17453 19382 0.11 + . g6 + scaffold1|size1992969 AUGUSTUS transcript 17453 19382 0.11 + . g6.t1 + scaffold1|size1992969 AUGUSTUS start_codon 17453 17455 . + 0 transcript_id "g6.t1"; gene_id "g6"; + scaffold1|size1992969 AUGUSTUS intron 17615 17660 0.38 + . transcript_id "g6.t1"; gene_id "g6"; + scaffold1|size1992969 AUGUSTUS intron 17708 17772 0.54 + . transcript_id "g6.t1"; gene_id "g6"; + scaffold1|size1992969 AUGUSTUS intron 17902 18035 0.58 + . transcript_id "g6.t1"; gene_id "g6"; + scaffold1|size1992969 AUGUSTUS intron 18313 18367 0.99 + . transcript_id "g6.t1"; gene_id "g6"; + scaffold1|size1992969 AUGUSTUS intron 19014 19080 0.44 + . transcript_id "g6.t1"; gene_id "g6"; + scaffold1|size1992969 AUGUSTUS CDS 17453 17614 0.55 + 0 transcript_id "g6.t1"; gene_id "g6"; + scaffold1|size1992969 AUGUSTUS CDS 17661 17707 0.38 + 0 transcript_id "g6.t1"; gene_id "g6"; + scaffold1|size1992969 AUGUSTUS CDS 17773 17901 0.54 + 1 transcript_id "g6.t1"; gene_id "g6"; + scaffold1|size1992969 AUGUSTUS CDS 18036 18312 0.52 + 1 transcript_id "g6.t1"; gene_id "g6"; + scaffold1|size1992969 AUGUSTUS CDS 18368 19013 0.99 + 0 transcript_id "g6.t1"; gene_id "g6"; + scaffold1|size1992969 AUGUSTUS CDS 19081 19379 0.31 + 2 transcript_id "g6.t1"; gene_id "g6"; + scaffold1|size1992969 AUGUSTUS stop_codon 19380 19382 . + 0 transcript_id "g6.t1"; gene_id "g6"; + + +**References** + +Mario Stanke and Stephan Waack (2003) +Gene Prediction with a Hidden-Markov Model and a new Intron Submodel. +Bioinformatics, Vol. 19, Suppl. 2, pages ii215-ii225 + + + + diff -r 5663d1a1b29c -r d5d5999ff4f1 augustus/augustus.xml --- a/augustus/augustus.xml Wed Mar 14 17:07:11 2012 +0100 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,186 +0,0 @@ - - gen prediction for eukaryotic genomes - augustus - --strand=$strand - $noInFrameStop - $gff - $protein - $introns - $start - $stop - $cds - $codingseq - $singlestrand - $input_genome - --genemodel=$genemodel - --species=$organism - --outfile=$output - - #please set export AUGUSTUS_CONFIG_PATH=/path_to_augustus/augustus/config - #or use the --AUGUSTUS_CONFIG_PATH=path switch - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - augustus - - - -**What it does** - -AUGUSTUS is a gene prediction program for eukaryotes written by Mario Stanke and Oliver Keller. -It can be used as an ab initio program, which means it bases its prediction purely on the -sequence. AUGUSTUS may also incorporate hints on the gene structure coming from extrinsic sources -such as EST, MS/MS, protein alignments and synthenic genomic alignments. - ------ - -**Parameters** - -Gene Model:: - - partial : allow prediction of incomplete genes at the sequence boundaries (default) - intronless : only predict single-exon genes like in prokaryotes and some eukaryotes - complete : only predict complete genes - atleastone : predict at least one complete gene - exactlyone : predict exactly one complete gene - - - -**Example** - -Suppose you have the following DNA formatted sequences:: - - >Seq1 - cccgcggagcgggtaccacatcgctgcgcgatgtgcgagcgaacacccgggctgcgcccg - ggtgttgcgctcccgctccgcgggagcgctggcgggacgctgcgcgtcccgctcaccaag - cccgcttcgcgggcttggtgacgctccgtccgctgcgcttccggagttgcggggcttcgc - cccgctaaccctgggcctcgcttcgctccgccttgggcctgcggcgggtccgctgcgctc - ccccgcctcaagggcccttccggctgcgcctccaggacccaaccgcttgcgcgggcctgg - -Running this tool will produce this:: - - # ----- prediction on sequence number 1 (length = 1992969, name = scaffold1|size1992969) ----- - # - # Constraints/Hints: - # (none) - # Predicted genes for sequence number 1 on both strands - # start gene g1 - scaffold1|size1992969 AUGUSTUS gene 17453 19382 0.11 + . g6 - scaffold1|size1992969 AUGUSTUS transcript 17453 19382 0.11 + . g6.t1 - scaffold1|size1992969 AUGUSTUS start_codon 17453 17455 . + 0 transcript_id "g6.t1"; gene_id "g6"; - scaffold1|size1992969 AUGUSTUS intron 17615 17660 0.38 + . transcript_id "g6.t1"; gene_id "g6"; - scaffold1|size1992969 AUGUSTUS intron 17708 17772 0.54 + . transcript_id "g6.t1"; gene_id "g6"; - scaffold1|size1992969 AUGUSTUS intron 17902 18035 0.58 + . transcript_id "g6.t1"; gene_id "g6"; - scaffold1|size1992969 AUGUSTUS intron 18313 18367 0.99 + . transcript_id "g6.t1"; gene_id "g6"; - scaffold1|size1992969 AUGUSTUS intron 19014 19080 0.44 + . transcript_id "g6.t1"; gene_id "g6"; - scaffold1|size1992969 AUGUSTUS CDS 17453 17614 0.55 + 0 transcript_id "g6.t1"; gene_id "g6"; - scaffold1|size1992969 AUGUSTUS CDS 17661 17707 0.38 + 0 transcript_id "g6.t1"; gene_id "g6"; - scaffold1|size1992969 AUGUSTUS CDS 17773 17901 0.54 + 1 transcript_id "g6.t1"; gene_id "g6"; - scaffold1|size1992969 AUGUSTUS CDS 18036 18312 0.52 + 1 transcript_id "g6.t1"; gene_id "g6"; - scaffold1|size1992969 AUGUSTUS CDS 18368 19013 0.99 + 0 transcript_id "g6.t1"; gene_id "g6"; - scaffold1|size1992969 AUGUSTUS CDS 19081 19379 0.31 + 2 transcript_id "g6.t1"; gene_id "g6"; - scaffold1|size1992969 AUGUSTUS stop_codon 19380 19382 . + 0 transcript_id "g6.t1"; gene_id "g6"; - - -**References** - -Mario Stanke and Stephan Waack (2003) -Gene Prediction with a Hidden-Markov Model and a new Intron Submodel. -Bioinformatics, Vol. 19, Suppl. 2, pages ii215-ii225 - - - - diff -r 5663d1a1b29c -r d5d5999ff4f1 augustus/readme.txt --- a/augustus/readme.txt Wed Mar 14 17:07:11 2012 +0100 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,78 +0,0 @@ -Galaxy wrapper for Augustus -===================================== - -This wrapper is copyright 2012 by Björn Grüning. - -This is a wrapper for the command line tool of augustus. -http://bioinf.uni-greifswald.de/augustus/ - -AUGUSTUS is a program that predicts genes in eukaryotic genomic sequences. - -Oliver Keller, Martin Kollmar, Mario Stanke, Stephan Waack (2011) -A novel hybrid gene prediction method employing protein multiple sequence alignments -Bioinformatics, doi: 10.1093/bioinformatics/btr010 - -Mario Stanke, Mark Diekhans, Robert Baertsch, David Haussler (2008) -Using native and syntenically mapped cDNA alignments to improve de novo gene finding -Bioinformatics, doi: 10.1093/bioinformatics/btn013 - -Mario Stanke and Stephan Waack (2003) -Gene Prediction with a Hidden-Markov Model and a new Intron Submodel. -Bioinformatics, Vol. 19, Suppl. 2, pages ii215-ii225 - - - - -Installation -============ - -Install or downlaod augustus from: - -http://bioinf.uni-greifswald.de/augustus/binaries/ - -and follow the installation instructions or copy the binaries into your $PATH - -To install the wrapper copy the augustus folder in the galaxy tools -folder and modify the tools_conf.xml file to make the tool available to Galaxy. -For example: - -
- -
- - -Set the AUGUSTUS_CONFIG_PATH to /path_to_augustus/augustus/config with - export AUGUSTUS_CONFIG_PATH=/path_to_augustus/augustus/config -or modify the wrapper and use the following additional commandline switch: - --AUGUSTUS_CONFIG_PATH=/path_to_augustus/augustus/config - - - - -History -======= - -v0.1 - Initial public release - - -Wrapper Licence (MIT/BSD style) -=============================== - -Permission to use, copy, modify, and distribute this software and its -documentation with or without modifications and for any purpose and -without fee is hereby granted, provided that any copyright notices -appear in all copies and that both those copyright notices and this -permission notice appear in supporting documentation, and that the -names of the contributors or copyright holders not be used in -advertising or publicity pertaining to distribution of the software -without specific prior permission. - -THE CONTRIBUTORS AND COPYRIGHT HOLDERS OF THIS SOFTWARE DISCLAIM ALL -WARRANTIES WITH REGARD TO THIS SOFTWARE, INCLUDING ALL IMPLIED -WARRANTIES OF MERCHANTABILITY AND FITNESS, IN NO EVENT SHALL THE -CONTRIBUTORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY SPECIAL, INDIRECT -OR CONSEQUENTIAL DAMAGES OR ANY DAMAGES WHATSOEVER RESULTING FROM LOSS -OF USE, DATA OR PROFITS, WHETHER IN AN ACTION OF CONTRACT, NEGLIGENCE -OR OTHER TORTIOUS ACTION, ARISING OUT OF OR IN CONNECTION WITH THE USE -OR PERFORMANCE OF THIS SOFTWARE. - diff -r 5663d1a1b29c -r d5d5999ff4f1 augustus/tool_conf.xml --- a/augustus/tool_conf.xml Wed Mar 14 17:07:11 2012 +0100 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,6 +0,0 @@ - - -
- -
-
diff -r 5663d1a1b29c -r d5d5999ff4f1 readme.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/readme.txt Thu Mar 15 10:09:28 2012 +0100 @@ -0,0 +1,78 @@ +Galaxy wrapper for Augustus +===================================== + +This wrapper is copyright 2012 by Björn Grüning. + +This is a wrapper for the command line tool of augustus. +http://bioinf.uni-greifswald.de/augustus/ + +AUGUSTUS is a program that predicts genes in eukaryotic genomic sequences. + +Oliver Keller, Martin Kollmar, Mario Stanke, Stephan Waack (2011) +A novel hybrid gene prediction method employing protein multiple sequence alignments +Bioinformatics, doi: 10.1093/bioinformatics/btr010 + +Mario Stanke, Mark Diekhans, Robert Baertsch, David Haussler (2008) +Using native and syntenically mapped cDNA alignments to improve de novo gene finding +Bioinformatics, doi: 10.1093/bioinformatics/btn013 + +Mario Stanke and Stephan Waack (2003) +Gene Prediction with a Hidden-Markov Model and a new Intron Submodel. +Bioinformatics, Vol. 19, Suppl. 2, pages ii215-ii225 + + + + +Installation +============ + +Install or downlaod augustus from: + +http://bioinf.uni-greifswald.de/augustus/binaries/ + +and follow the installation instructions or copy the binaries into your $PATH + +To install the wrapper copy the augustus folder in the galaxy tools +folder and modify the tools_conf.xml file to make the tool available to Galaxy. +For example: + +
+ +
+ + +Set the AUGUSTUS_CONFIG_PATH to /path_to_augustus/augustus/config with + export AUGUSTUS_CONFIG_PATH=/path_to_augustus/augustus/config +or modify the wrapper and use the following additional commandline switch: + --AUGUSTUS_CONFIG_PATH=/path_to_augustus/augustus/config + + + + +History +======= + +v0.1 - Initial public release + + +Wrapper Licence (MIT/BSD style) +=============================== + +Permission to use, copy, modify, and distribute this software and its +documentation with or without modifications and for any purpose and +without fee is hereby granted, provided that any copyright notices +appear in all copies and that both those copyright notices and this +permission notice appear in supporting documentation, and that the +names of the contributors or copyright holders not be used in +advertising or publicity pertaining to distribution of the software +without specific prior permission. + +THE CONTRIBUTORS AND COPYRIGHT HOLDERS OF THIS SOFTWARE DISCLAIM ALL +WARRANTIES WITH REGARD TO THIS SOFTWARE, INCLUDING ALL IMPLIED +WARRANTIES OF MERCHANTABILITY AND FITNESS, IN NO EVENT SHALL THE +CONTRIBUTORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY SPECIAL, INDIRECT +OR CONSEQUENTIAL DAMAGES OR ANY DAMAGES WHATSOEVER RESULTING FROM LOSS +OF USE, DATA OR PROFITS, WHETHER IN AN ACTION OF CONTRACT, NEGLIGENCE +OR OTHER TORTIOUS ACTION, ARISING OUT OF OR IN CONNECTION WITH THE USE +OR PERFORMANCE OF THIS SOFTWARE. + diff -r 5663d1a1b29c -r d5d5999ff4f1 tool_conf.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_conf.xml Thu Mar 15 10:09:28 2012 +0100 @@ -0,0 +1,6 @@ + + +
+ +
+