Mercurial > repos > bjoern-gruening > diffbind
diff diffbind.R @ 0:c0f5b2133506 draft default tip
initial commit for DiffBind
author | bjoern-gruening |
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date | Tue, 14 Jan 2014 18:12:02 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/diffbind.R Tue Jan 14 18:12:02 2014 -0500 @@ -0,0 +1,50 @@ +## Setup R error handling to go to stderr +options( show.error.messages=F, error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) +# we need that to not crash galaxy with an UTF8 error on German LC settings. +Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") + +library('getopt'); +options(stringAsfactors = FALSE, useFancyQuotes = FALSE) +args <- commandArgs(trailingOnly = TRUE) + +#get options, using the spec as defined by the enclosed list. +#we read the options from the default: commandArgs(TRUE). +spec = matrix(c( + 'verbose', 'v', 2, "integer", + 'help' , 'h', 0, "logical", + 'outfile' , 'o', 1, "character", + 'plots' , 'p', 2, "character", + 'infile' , 'i', 1, "character", + 'format', 'f', 1, 'character' +), byrow=TRUE, ncol=4); + +opt = getopt(spec); + +# if help was asked for print a friendly message +# and exit with a non-zero error code +if ( !is.null(opt$help) ) { + cat(getopt(spec, usage=TRUE)); + q(status=1); +} + + +library('DiffBind') +# used to save to BED, GFF or WIG format +library('rtracklayer') + +if ( !is.null(opt$plots) ) { + pdf(opt$plots) +} + + +sample = dba(sampleSheet=opt$infile, peakFormat='bed') +sample_count = dba.count(sample) +sample_contrast = dba.contrast(sample_count, categories=DBA_CONDITION) +sample_analyze = dba.analyze(sample_contrast) +diff_bind = dba.report(sample_analyze) + + +export(diff_bind, opt$outfile, format=opt$format) + +dev.off() +sessionInfo()