Mercurial > repos > bjoern-gruening > diffbind
diff diffbind.xml @ 0:c0f5b2133506 draft default tip
initial commit for DiffBind
author | bjoern-gruening |
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date | Tue, 14 Jan 2014 18:12:02 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/diffbind.xml Tue Jan 14 18:12:02 2014 -0500 @@ -0,0 +1,91 @@ +<tool id="diffbind" name="DiffBind" version="2.0.1"> + <description> differential binding analysis of ChIP-Seq peak data</description> + <requirements> + <requirement type="binary">Rscript</requirement> + <requirement type="R-module">DESeq2</requirement> + <requirement type="package" version="3.0.1">R_3_0_1</requirement> + <requirement type="package" version="1.0.17">deseq2</requirement> + <requirement type="package" version="1.8.3">diffbind</requirement> + </requirements> + <command interpreter="Rscript"> + diffbind.R + -i $infile + -o $outfile + -p $plots + -f $format + ; + cat $infile; + </command> + <stdio> + <regex match="Execution halted" + source="both" + level="fatal" + description="Execution halted." /> + <regex match="Input-Error 01" + source="both" + level="fatal" + description="Error in your input parameters: Make sure you only apply factors to selected samples." /> + <regex match="Error in" + source="both" + level="fatal" + description="An undefined error occured, please check your intput carefully and contact your administrator." /> + </stdio> + <inputs> + <repeat name="samples" title="Samples" min="2"> + <param name="sample_id" type="text" value="Sample ID" label="Specify a sample id" help="e.g. BT474.1-" /> + <param name="tissue" type="text" value="Tissue" label="Specify the tissue" help="e.g. BT474" /> + <param name="factor" type="text" value="Factor Name" label="Specify a factor name" help="e.g. ER" /> + <param name="condition" type="text" value="Condition" label="Specify the condition" help="e.g. Resistent" /> + <param name="replicate" type="integer" value="1" label="Specify the replicate number" help="e.g. 1" /> + <param format="bam" name="bamreads" type="data" label="Read BAM file" help="Specify the Read BAM file, used for Peak calling."/> + <param format="bam" name="bamcontrol" type="data" label="Control BAM file" help="Specify the Control BAM file, used for Peak calling."/> + <param format="bed" name="peaks" type="data" label="Peak file" help="Result of your Peak calling experiment."/> + </repeat> + <param name="pdf" type="boolean" truevalue="" falsevalue="" checked="true" + label="Visualising the analysis results" + help="output an additional PDF files" /> + <param name="format" type="select" label="Output Format"> + <option value="bed">BED</option> + <option value="gff">GFF</option> + <option value="wig">WIG</option> + </param> + </inputs> + <configfiles> +<configfile name="infile"> +SampleID,Tissue,Factor,Condition,Replicate,bamReads,bamControl,Peaks +#for $sample in $samples: +$sample.sample_id,$sample.tissue,$sample.factor,$sample.condition,$sample.replicate,$sample.bamreads,$sample.bamcontrol,$sample.peaks +#end for +</configfile> + </configfiles> + <outputs> + <data format="bed" name="outfile" label="Differential binding sites on ${on_string}"> + <change_format> + <when input="format" value="wig" format="wig" /> + <when input="format" value="gff" format="gff" /> + </change_format> + </data> + <data format="pdf" name="plots" label="Differential binding sites on ${on_string}"> + <filter>pdf == True</filter> + </data> + </outputs> + + <help> + +.. class:: infomark + +**What it does** + + +------ + +**References** + +DiffBind_ Authors: Rory Stark, Gordon Brown + +.. _DiffBind: http://www.bioconductor.org/packages/release/bioc/html/DiffBind.html + +Wrapper authors: Bjoern Gruening, Pavankumar Videm + + </help> +</tool>