Mercurial > repos > bornea > dotplot_runner
view Dotplot_Release/SOFD.pl @ 4:a0b4bcf67972 draft
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author | bornea |
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date | Fri, 29 Jan 2016 10:00:21 -0500 |
parents | dfa3436beb67 |
children |
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#!/usr/bin/perl # 17/12/2013 if($#ARGV==0){ print "This program takes the Saint Output File and produces two matrices.\n"; print "One has average spectral counts and the other FDR scores,\n"; print "\nusage:\n $0\n-i [csv saint output file]\n-s [FDR cutoff, default=0.01]\n\n"; die; } else{ $i=0; $cutoff=0.01; $spec_cutoff=0; while($i<=$#ARGV){ if($ARGV[$i] eq '-i'){ $i++; $ifile=$ARGV[$i]; } elsif($ARGV[$i] eq '-s'){ $i++; if($ARGV[$i]>1 || $ARGV[$i]<0){ die "\nFDR cutoff must be between 0 and 1 \n\n"; } $cutoff=$ARGV[$i]; } elsif($ARGV[$i] eq '-x'){ $i++; if($ARGV[$i]<0){ die "\nAvgSpec cutoff must be > 0 \n\n"; } $spec_cutoff=$ARGV[$i]; } else{ die "\Incorrect program usage\n\n"; } $i++; } } $baitn=0, $bait[0]=xxxx, $sig_preysn=0; $file=''; open(IFILE,"<$ifile") || die "$ifile can't be opened: $!"; { local $/=undef; $file=<IFILE>; } @lines=split /[\r\n]+/, $file; foreach $line (@lines) { if($line =~ /^Bait/){ } elsif($line =~ /^([^\t]+)\t[^\t]+\t([^\t]+)\t[^\t]+\t[\d]+\t([\d\.]+)\t[\d]+\t[^\t]+\t[^\t]+\t[^\t]+\t[^\t]+\t[^\t]+\t([^\t]+)\t[^\t]+\t([^\t]+)\t/){ if($1 ne $bait[$baitn]){ $baitn++; $bait[$baitn]=$1; $preyn[$baitn]=0; } $preyn[$baitn]++; $preys[$baitn][$preyn[$baitn]]=$2; $avgspec[$baitn][$preyn[$baitn]]=$3; $saint[$baitn][$preyn[$baitn]]=$4; $fdr[$baitn][$preyn[$baitn]]=$5; if($5 <= $cutoff && $3 >= $spec_cutoff){ $check_prey=0; for($i=1; $i<=$sig_preysn; $i++){ if($sig_preys[$i] eq $2){ $check_prey=1; } } if($check_prey==0){ $sig_preysn++; $sig_preys[$sig_preysn]=$2; } } } else{ } } close(IFILE); open(SC_FILE, ">SC_data.txt"); open(FDR_FILE, ">FDR_data.txt"); for($i=1; $i<=$baitn; $i++){ print SC_FILE "\t$bait[$i]"; print FDR_FILE "\t$bait[$i]"; } print SC_FILE "\n"; print FDR_FILE "\n"; for($i=1; $i<=$sig_preysn; $i++){ print SC_FILE "$sig_preys[$i]"; print FDR_FILE "$sig_preys[$i]"; for($j=1; $j<=$baitn; $j++){ $krem=0; for($k=1; $k<=$preyn[$j]; $k++){; if($preys[$j][$k] eq $sig_preys[$i]){ $krem=$k; last; } } if($krem != 0){ print SC_FILE "\t$avgspec[$j][$krem]"; print FDR_FILE "\t$fdr[$j][$krem]"; } else{ print SC_FILE "\t0"; print FDR_FILE "\t1"; } } print SC_FILE "\n"; print FDR_FILE "\n"; } close(SC_FILE); close(FDR_FILE);