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1 import sys
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2 import os
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3 from time import sleep
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4
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5 files = sys.argv[1] # read in a string of file names seperated by ", "
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6 # e.g. "Default_Protein_Report.txt, Default_Protein_Report_2.txt"
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7 #bait = sys.argv[2] # SAINT formatted bait file
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8 # still need a way to match files to bait identifiers
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9 # or they can just be required to be put in the order of the bait file
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10 quant_type = sys.argv[3] # what metric to use for quantification
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11 # "#Validated Peptides", "#Peptides", "#Unique", "#Validated PSMs", "#PSMs"
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12 db = sys.argv[4] # fasta database used in SearchGUI and PeptideShaker
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13 prey = sys.argv[5]
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14 tool_path = sys.argv[7]
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15 if fasta_db == "None":
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16 fasta_db = str(tool_path) + "/SwissProt_HUMAN_2015_12.fasta"
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17 make_bait = sys.argv[6]
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18 bait_bool = sys.argv[8]
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19
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20 def bait_create(baits, infile):
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21 # Verifies the Baits are valid in the Scaffold file and writes the Bait.txt.
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22 baits = make_bait.split()
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23 i = 0
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24 bait_file_tmp = open("bait.txt", "w")
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25 order = []
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26 bait_cache = []
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27 while i < len(baits):
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28 if baits[i+2] == "true":
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29 T_C = "C"
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30 else:
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31 T_C = "T"
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32 bait_line = baits[i] + "\t" + baits[i+1] + "\t" + T_C + "\n"
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33 bait_cache.append(str(bait_line))
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34 i = i + 3
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35
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36 for cache_line in bait_cache:
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37 bait_file_tmp.write(cache_line)
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38
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39 bait_file_tmp.close()
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40
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41 if bait_bool == 'false':
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42 bait_create(make_bait, infile)
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43 bait = "bait.txt"
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44 else:
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45 bait_temp_file = open(sys.argv[9], 'r')
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46 bait_cache = bait_temp_file.readlines()
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47 bait_file_tmp = open("bait.txt", "wr")
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48 for cache_line in bait_cache:
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49 bait_file_tmp.write(cache_line)
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50 bait_file_tmp.close()
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51 bait = "bait.txt"
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52
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53 class ReturnValue1(object):
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54 def __init__(self, sequence, gene):
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55 self.seqlength = sequence
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56 self.genename = gene
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57
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58 def read_tab(infile):
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59 with open(infile,'r') as x:
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60 output = []
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61 for line in x:
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62 line = line.strip()
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63 temp = line.split('\t')
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64 output.append(temp)
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65 return output
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66 def printProgress (iteration, total, prefix = '', suffix = '', decimals = 1, barLength = 100):
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67 """
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68 Call in a loop to create terminal progress bar
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69 @params:
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70 iteration - Required : current iteration (Int)
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71 total - Required : total iterations (Int)
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72 prefix - Optional : prefix string (Str)
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73 suffix - Optional : suffix string (Str)
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74 decimals - Optional : positive number of decimals in percent complete (Int)
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75 barLength - Optional : character length of bar (Int)
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76 """
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77 formatStr = "{0:." + str(decimals) + "f}"
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78 percents = formatStr.format(100 * (iteration / float(total)))
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79 filledLength = int(round(barLength * iteration / float(total)))
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80 bar = '=' * filledLength + '-' * (barLength - filledLength)
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81 sys.stdout.write('\r%s |%s| %s%s %s' % (prefix, bar, percents, '%', suffix)),
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82 sys.stdout.flush()
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83 if iteration == total:
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84 sys.stdout.write('\n')
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85 sys.stdout.flush()
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86 def get_info(uniprot_accession_in,fasta_db):
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87 # Get aminoacid lengths and gene name.
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88 error = open('error proteins.txt', 'a+')
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89 data = open(fasta_db, 'r')
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90 data_lines = data.readlines()
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91 db_len = len(data_lines)
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92 seqlength = 0
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93 count = 0
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94 for data_line in data_lines:
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95 if ">sp" in data_line:
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96 namer = data_line.split("|")[2]
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97 if uniprot_accession_in == data_line.split("|")[1]:
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98 match = count + 1
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99 if 'GN=' in data_line:
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100 lst = data_line.split('GN=')
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101 lst2 = lst[1].split(' ')
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102 genename = lst2[0]
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103 if 'GN=' not in data_line:
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104 genename = 'NA'
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105 while ">sp" not in data_lines[match]:
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106 if match <= db_len:
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107 seqlength = seqlength + len(data_lines[match].strip())
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108 match = match + 1
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109 else:
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110 break
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111 return ReturnValue1(seqlength, genename)
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112 if uniprot_accession_in == namer.split(" ")[0]:
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113 match = count + 1
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114 # Ensures consistent spacing throughout.
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115 if 'GN=' in data_line:
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116 lst = data_line.split('GN=')
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117 lst2 = lst[1].split(' ')
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118 genename = lst2[0]
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119 if 'GN=' not in data_line:
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120 genename = 'NA'
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121 while ">sp" not in data_lines[match]:
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122 if match <= db_len:
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123 seqlength = seqlength + len(data_lines[match].strip())
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124 match = match + 1
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125 else:
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126 break
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127 return ReturnValue1(seqlength, genename)
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128 count = count + 1
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129 if seqlength == 0:
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130 error.write(uniprot_accession_in + '\t' + "Uniprot not in Fasta" + '\n')
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131 error.close
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132 seqlength = 'NA'
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133 genename = 'NA'
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134 return ReturnValue1(seqlength, genename)
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135 def concatenate_files(file_list_string, bait_file):
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136 file_list = file_list_string.split(", ")
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137 bait = read_tab(bait_file)
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138 master_table = []
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139 header_check = 0
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140 file_cnt = 0
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141 table_cnt = 0
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142 for i in file_list:
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143 table = read_tab(i)
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144 for j in table:
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145 if table_cnt == 0:
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146 if header_check == 0:
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147 header_check +=1
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148 j.append("Replicate")
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149 j.append("Bait_Grouping")
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150 master_table.append(j)
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151 if table_cnt > 0:
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152 j.append(bait[file_cnt][0])
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153 j.append(bait[file_cnt][1])
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154 master_table.append(j)
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155 table_cnt +=1
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156 file_cnt+=1
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157 table_cnt = 0
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158 if len(master_table[0]) < len(master_table[1]):
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159 master_table[0] = ["#"] + master_table[0]
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160 with open("merged_PeptideShaker.txt","w") as x:
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161 for i in master_table:
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162 x.write("\t".join(i))
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163 x.write("\n")
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164 return master_table
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165 def make_inter(master_table,quant_type):
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166 if len(master_table[0]) < len(master_table[1]):
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167 master_table[0] = ["#"] + master_table[0]
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168 replicate_index = master_table[0].index("Replicate")
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169 grouping_index = master_table[0].index("Bait_Grouping")
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170 accession_index = master_table[0].index("Main Accession")
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171 Quant_index = master_table[0].index(quant_type)
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172 inter_file = ""
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173 for i in master_table[1:]:
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174 line = []
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175 line.append(i[replicate_index])
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176 line.append(i[grouping_index])
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177 line.append(i[accession_index])
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178 line.append(i[Quant_index])
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179 inter_file = inter_file + "\t".join(line) + "\n"
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180 with open("inter.txt","w") as x:
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181 x.write(inter_file)
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182
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183 def make_prey(concat_table,fasta_db):
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184 input_data = concat_table
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185 if len(input_data[0]) < len(input_data[1]):
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186 input_data[0] = ["#"] + input_data[0]
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187 accession_index = input_data[0].index("Main Accession")
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188 proteins = []
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189 for i in input_data[1:]:
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190 proteins.append(i[accession_index])
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191 output_file = open("prey.txt", 'w')
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192 start = 0
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193 end = len(proteins)
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194
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195 # Initial call to print 0% progress
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196 printProgress(start, end, prefix = 'Progress:', suffix = 'Complete', barLength = 50)
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197
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198 for protein in proteins:
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199 seq = get_info(protein,fasta_db).seqlength
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200 GN = get_info(protein,fasta_db).genename
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201 if seq != 'NA':
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202 output_file.write(protein + "\t" + str(seq) + "\t" + str(GN) + "\n")
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203 start+=1
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204 printProgress(start, end, prefix = 'Progress:', suffix = 'Complete', barLength = 50)
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205 output_file.close()
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206 data = concatenate_files(files,bait)
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207 make_inter(data, quant_type)
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208 if prey == "true":
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209 make_prey(data,db)
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210
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211 os.rename("bait.txt", sys.argv[2])
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212 os.rename("inter.txt", sys.argv[10])
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213 os.rename("prey.txt", sys.argv[11]) |
