Mercurial > repos > brad-chapman > bam_to_bigwig
diff bam_to_bigwig/bam_to_bigwig.xml @ 2:e2edfa478eb4
Covert to use bedGraph intermediate with bedtools. Thanks to Peter Cock and Lance Parsons for the updates
author | Brad Chapman <chapmanb@50mail.com> |
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date | Wed, 05 Sep 2012 21:14:54 -0400 |
parents | 0ff100a057ef |
children | 14e5258d1b39 |
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--- a/bam_to_bigwig/bam_to_bigwig.xml Tue Jun 07 16:26:46 2011 -0400 +++ b/bam_to_bigwig/bam_to_bigwig.xml Wed Sep 05 21:14:54 2012 -0400 @@ -1,17 +1,23 @@ -<tool id="bam_to_bigwig" name="BAM to BigWig" version="0.0.2"> +<tool id="bam_to_bigwig" name="BAM to BigWig" version="0.1.0"> <description>Calculates coverage from a BAM alignment file</description> - <command interpreter="python">bam_to_wiggle.py $align --outfile=$out</command> + <command interpreter="python">bam_to_bigwig.py $align --outfile=$out</command> <inputs> <param format="bam" name="align" type="data" label="BAM alignment file"/> </inputs> <outputs> <data format="bigwig" name="out" /> </outputs> - + <requirements> + <requirement type="python-module">pysam</requirement> + <requirement type="binary">genomeCoverageBed</requirement> + <requirement type="binary">bedGraphToBigWig</requirement> + </requirements> <help> **What it does** -Creates a coverage file in BigWig format, given a BAM alignment file. +Creates a coverage file in BigWig format, given a BAM alignment file. + +Gaps or skips (CIGAR D or N operators) are not counted towards the coverage calculation, which is important when mapping RNA Seq reads to genes with introns. **Input** @@ -21,5 +27,4 @@ BigWig files can be loaded directly from Galaxy into the UCSC browser. They can be loaded incrementally by UCSC, so a single file can be used to represent the entire genome without having to upload the entire thing as a custom track. </help> - </tool>