Mercurial > repos > brad-chapman > bam_to_bigwig
diff bam_to_bigwig.xml @ 6:9163e1db4c16 draft default tip
planemo upload for repository https://github.com/lparsons/galaxy_tools/tree/master/tools/bam_to_bigwig/ commit b5c3df21127fc27f66a97761173c07e161f2fa8e
author | lparsons |
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date | Thu, 12 May 2016 11:39:13 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/bam_to_bigwig.xml Thu May 12 11:39:13 2016 -0400 @@ -0,0 +1,58 @@ +<tool id="bam_to_bigwig" name="BAM to BigWig" version="0.2.0"> + + <description>Calculates coverage from a BAM alignment file</description> + + <requirements> + <requirement type="package" version="0.8.3">pysam</requirement> + <requirement type="package" version="2.24">bedtools</requirement> + <requirement type="package" version="312">ucsc_tools</requirement> + </requirements> + + <command detect_errors="aggressive" interpreter="python"><![CDATA[ + bam_to_bigwig.py $align --outfile=$out --split + ]]></command> + + <inputs> + <param format="bam" name="align" type="data" label="BAM alignment file"/> + </inputs> + + <outputs> + <data format="bigwig" name="out" /> + </outputs> + + <tests> + <test> + <param name="align" value="bam_to_bigwig_test.bam"/> + <output name="out" file="bam_to_bigwig_test.bigwig"/> + </test> + </tests> + +<help><![CDATA[ +**What it does** + +Creates a coverage file in BigWig format, given a BAM alignment file. + +Gaps or skips (CIGAR D or N operators) are not counted towards the coverage +calculation, which is important when mapping RNA Seq reads to genes with +introns. + +**Input** + +A BAM alignment file. This needs to have the genome database build used in +alignment annotated. If your file has '?' for the database build, click on the +pencil icon to edit the alignment attributes, and specify the organism used to +align against. + +**Output** + +BigWig files can be loaded directly from Galaxy into the UCSC browser. They can +be loaded incrementally by UCSC, so a single file can be used to represent the +entire genome without having to upload the entire thing as a custom track. + +]]></help> + + <citations> + <citation type="doi">10.1093/bioinformatics/btp352</citation> + <citation type="doi">10.1093/bioinformatics/btq033</citation> + </citations> +</tool>