Mercurial > repos > brasset_jensen > srnapipe
diff bin/resize.pm @ 61:9185ca0a7b43 draft
Updated package according to recommendations.
author | pierre.pouchin |
---|---|
date | Wed, 16 Jan 2019 08:18:13 -0500 |
parents | 4bc00caa60b4 |
children |
line wrap: on
line diff
--- a/bin/resize.pm Wed Oct 24 07:40:20 2018 -0400 +++ b/bin/resize.pm Wed Jan 16 08:18:13 2019 -0500 @@ -1,101 +1,101 @@ -package resize; - -use strict; -use warnings; - -use FindBin; -use lib $FindBin::Bin; -use Rcall qw ( histogram ); - -use Exporter; -our @ISA = qw( Exporter ); -our @EXPORT_OK = qw( &size_distribution ); - -sub size_distribution -{ - my ( $fastq, $fastq_out, $dir, $min, $max ) = @_; - - my ( %fragments_size, %duplicates ) ; - my $num = size($min, $max, $fastq, $fastq_out, \%fragments_size, \%duplicates); - - my $png = $dir.'histogram.png'; - histogram(\%fragments_size, $png, $num); - - my $size = $dir.'reads_size.txt'; - - - my $pourcentage; - open my $o, '>', $size || die "cannot open $size $!\n"; - print $o "size\tnumber\tpercentage\n"; - foreach my $k (sort { $a <=> $b } keys %fragments_size ) - { - $pourcentage = $fragments_size{$k} / $num * 100; - - print $o "$k\t$fragments_size{$k}\t"; - printf $o "%.2f\n",$pourcentage; - } - close $o; - - my $dup = $dir.'duplicates.txt' ; - open $o, '>', $dup || die "cannot open $size $!\n"; - print $o "size\tnumber\n"; - foreach my $k (sort { $duplicates{$b} <=> $duplicates{$a} } keys %duplicates ) - { - print $o "$k\t$duplicates{$k}\n"; - } - close $o; -} - -sub size -{ - my ($min, $max, $in_file, $out_file, $sizeHashR, $duplicateHashR) = @_; - my ($numreads, $size, $cmp, $ok, $line) = (0, 0, 0, 0); - my @fastq; - open (my $in, $in_file) || die "cannot open $in_file $!\n"; - open (my $out, ">".$out_file) || die "cannot create $out_file $!\n"; - while(<$in>) - { - chomp $_; - $cmp++; $line++; - if ($cmp == 1) - { - die "file do not contain a @ at line $line\n" unless ($_ =~ /^\@/ ); - $ok = 0; @fastq = (); - push(@fastq,$_); - } - elsif ($cmp == 2) - { - #die "unrecognized symbol at line $line\n" unless ($_ =~ /[atcgATCGnN]+/ || $_ =~ /^$/ ); - push(@fastq,$_); - $size = length($_); - if ($size >= $min && $size <= $max) - { - $numreads++; - ${$sizeHashR}{$size}+=1; - ${$duplicateHashR}{$_}+=1 if (defined($duplicateHashR)); - $ok = 1; - } - } - elsif ($cmp == 3 ) - { - die "file do not contain a + at line $line\n" unless $_ =~ /^\+/; - push(@fastq,$_); - } - elsif ($cmp == 4 ) - { - push(@fastq,$_); - $cmp = 0; - if ($ok == 1) - { - foreach my $t (@fastq) - { - print $out $t."\n"; - } - } - } - } - close $in; close $out; - return $numreads; -} - -1; +package resize; + +use strict; +use warnings; + +use FindBin; +use lib $FindBin::Bin; +use Rcall qw ( histogram ); + +use Exporter; +our @ISA = qw( Exporter ); +our @EXPORT_OK = qw( &size_distribution ); + +sub size_distribution +{ + my ( $fastq, $fastq_out, $dir, $min, $max ) = @_; + + my ( %fragments_size, %duplicates ) ; + my $num = size($min, $max, $fastq, $fastq_out, \%fragments_size, \%duplicates); + + my $png = $dir.'histogram.png'; + histogram(\%fragments_size, $png, $num); + + my $size = $dir.'reads_size.txt'; + + + my $pourcentage; + open my $o, '>', $size || die "cannot open $size $!\n"; + print $o "size\tnumber\tpercentage\n"; + foreach my $k (sort { $a <=> $b } keys %fragments_size ) + { + $pourcentage = $fragments_size{$k} / $num * 100; + + print $o "$k\t$fragments_size{$k}\t"; + printf $o "%.2f\n",$pourcentage; + } + close $o; + + my $dup = $dir.'duplicates.txt' ; + open $o, '>', $dup || die "cannot open $size $!\n"; + print $o "size\tnumber\n"; + foreach my $k (sort { $duplicates{$b} <=> $duplicates{$a} } keys %duplicates ) + { + print $o "$k\t$duplicates{$k}\n"; + } + close $o; +} + +sub size +{ + my ($min, $max, $in_file, $out_file, $sizeHashR, $duplicateHashR) = @_; + my ($numreads, $size, $cmp, $ok, $line) = (0, 0, 0, 0); + my @fastq; + open (my $in, $in_file) || die "cannot open $in_file $!\n"; + open (my $out, ">".$out_file) || die "cannot create $out_file $!\n"; + while(<$in>) + { + chomp $_; + $cmp++; $line++; + if ($cmp == 1) + { + die "file do not contain a @ at line $line\n" unless ($_ =~ /^\@/ ); + $ok = 0; @fastq = (); + push(@fastq,$_); + } + elsif ($cmp == 2) + { + #die "unrecognized symbol at line $line\n" unless ($_ =~ /[atcgATCGnN]+/ || $_ =~ /^$/ ); + push(@fastq,$_); + $size = length($_); + if ($size >= $min && $size <= $max) + { + $numreads++; + ${$sizeHashR}{$size}+=1; + ${$duplicateHashR}{$_}+=1 if (defined($duplicateHashR)); + $ok = 1; + } + } + elsif ($cmp == 3 ) + { + die "file do not contain a + at line $line\n" unless $_ =~ /^\+/; + push(@fastq,$_); + } + elsif ($cmp == 4 ) + { + push(@fastq,$_); + $cmp = 0; + if ($ok == 1) + { + foreach my $t (@fastq) + { + print $out $t."\n"; + } + } + } + } + close $in; close $out; + return $numreads; +} + +1;