Mercurial > repos > bvalot > pymlst
view clamlst_search.xml @ 2:4394da33d837 draft default tip
planemo upload for repository https://github.com/bvalot/pyMLST commit 142042a3856f8886031cacaa4fb28743a1bcd540
author | bvalot |
---|---|
date | Wed, 15 Nov 2023 12:22:07 +0000 |
parents | 5b2c48fa0175 |
children |
line wrap: on
line source
<tool id="clamlst_search_wrapper" name="Search MLST database" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="20.05"> <description></description> <macros> <import>macro.xml</import> </macros> <expand macro="requirements"> <requirement type="package" version="4.8">sed</requirement> </expand> <stdio> <exit_code range="1:" level="fatal" /> </stdio> <version_command>claMLST -v</version_command> <command><![CDATA[ #set $databaselink = $database.file_name.split("/")[-1] ln -s $database.file_name \$_GALAXY_JOB_TMP_DIR/ && claMLST #if $data.input == "fasta" search #else search2 #end if #if str($coverage) -c $coverage #end if #if str($identity) -i $identity #end if #if $fasta -f '${fastaout}' #end if #if $data.input == "fasta" \$_GALAXY_JOB_TMP_DIR/'${databaselink}' #for $datain in $data.fastain: '${datain}' #end for #for $datain in $data.fastain: #set $name = $datain.file_name.split("/")[-1].rstrip(".dat") | sed -e "~s/$name/$datain.name/" #end for #end if #if $data.input == "fastqsingle" #if str($data.reads) -r $data.reads #end if --single \$_GALAXY_JOB_TMP_DIR/'${databaselink}' #for $datain in $data.single: '${datain}' #end for #for $datain in $data.single: #set $name = $datain.file_name.split("/")[-1].rstrip(".dat") | sed -e "~s/$name/$datain.name/" #end for #end if #if $data.input == "fastqpaired" #if str($data.reads) -r $data.reads #end if --paired \$_GALAXY_JOB_TMP_DIR/'${databaselink}' '${data.pairedfile.forward}' '${data.pairedfile.reverse}' #set $name = $data.pairedfile.forward.file_name.split("/")[-1].rstrip(".dat") | sed -e "~s/$name/$data.pairedfile.name/" #end if #if $data.input == "fastqpaired2" #if str($data.reads) -r $data.reads #end if --paired \$_GALAXY_JOB_TMP_DIR/'${databaselink}' #for $datain in $data.pairedfilelist: '${datain.forward}' '${datain.reverse}' #end for #for $datain in $data.pairedfilelist: #set $name = $datain.forward.file_name.split("/")[-1].rstrip(".dat") | sed -e "~s/$name/$datain.name/" #end for #end if > '${mlst}' #if $log 2> '${logfile}' #end if ]]> </command> <inputs> <param name="database" type="data" format="sqlite" label="Classical MLST database" help="Sqlite format from claMLST import" /> <conditional name="data"> <param name="input" type="select" label="Select type of data"> <option value="fasta" selected="true">Assembly (fasta)</option> <option value="fastqsingle">Raw data (single)</option> <option value="fastqpaired">Raw data (paired)</option> <option value="fastqpaired2">Raw data (List of paired)</option> </param> <when value="fasta"> <param name="fastain" type="data" format="fasta" label="Assembly Genome" help="Fasta format" multiple="true" /> </when> <when value="fastqsingle"> <param name="single" type="data" format="fastq,fastq.gz" label="Single read file" help="Fastq(gz) format" multiple="true" /> <param name="reads" type="integer" value="10" optional="true" label="Minimum reads coverage to search gene" /> </when> <when value="fastqpaired"> <param name="pairedfile" type="data_collection" format="fastq,fastq.gz" label="Paired of read files" help="Fastq(gz) format" collection_type="paired" /> <param name="reads" type="integer" value="10" optional="true" label="Minimum reads coverage to search gene" /> </when> <when value="fastqpaired2"> <param name="pairedfilelist" type="data_collection" format="fastq,fastq.gz" label="Paired of read files" help="Fastq(gz) format" collection_type="list:paired" /> <param name="reads" type="integer" value="10" optional="true" label="Minimum reads coverage to search gene" /> </when> </conditional> <param name="fasta" type="boolean" checked="false" label="Write fasta file with MLST genes allele" /> <param name="identity" type="float" value="0.9" optional="false" label="Identity" help="Minimum identity to search gene" /> <param name="coverage" type="float" value="0.9" optional="false" label="Coverage" help="Minimum coverage to search gene" /> <param name="log" type="boolean" checked="false" label="Write log file" /> </inputs> <outputs> <data name="logfile" format="txt" label="${tool.name} on ${on_string}: log"> <filter>log</filter> </data> <data name="mlst" format="txt" label="${tool.name} on ${on_string}: mlst" /> <data name="fastaout" format="fasta" label="${tool.name} on {on_string}: fasta"> <filter>fasta</filter> </data> </outputs> <tests> <test expect_num_outputs="1"> <param name="database" value="clamlst_ecoli.db" /> <conditional name="data"> <param name="input" value="fasta" /> <param name="fastain" ftype="fasta" value="EHSB-021.fasta,EHSB-092.fasta" /> </conditional> <param name="identity" value="0.8" /> <output name="mlst" ftype="txt"> <assert_contents> <has_text_matching expression="155\t6\t4\t14\t16\t24\t8\t14" /> <has_text_matching expression="3018\t12\t58\t54\t344\t1\t2\t47" /> </assert_contents> </output> </test> <test expect_num_outputs="1"> <param name="database" value="clamlst_ecoli.db" /> <conditional name="data"> <param name="input" value="fastqsingle" /> <param name="single" ftype="fastqsanger" value="EHSB-021_R1.fastq.gz,EHSB-021_R2.fastq.gz" /> </conditional> <param name="identity" value="0.8" /> <param name="reads" value="5" /> <output name="mlst" ftype="txt"> <assert_contents> <has_text_matching expression="14\t16\t24\t8" /> </assert_contents> </output> </test> <test expect_num_outputs="1"> <param name="database" value="clamlst_ecoli.db" /> <conditional name="data"> <param name="input" value="fastqpaired" /> <param name="pairedfile"> <collection type="paired"> <element name="forward" value="EHSB-021_R1.fastq.gz" /> <element name="reverse" value="EHSB-021_R2.fastq.gz" /> </collection> </param> <param name="reads" value="5" /> </conditional> <output name="mlst" ftype="txt"> <assert_contents> <has_text_matching expression="4\t14\t16\t24\t8\t14" /> </assert_contents> </output> </test> <test expect_num_outputs="3"> <param name="database" value="clamlst_ecoli.db" /> <conditional name="data"> <param name="input" value="fastqpaired2" /> <param name="pairedfilelist"> <collection type="list:paired"> <element name="EHSB-021"> <collection type="paired"> <element name="forward" value="EHSB-021_R1.fastq.gz" /> <element name="reverse" value="EHSB-021_R2.fastq.gz" /> </collection> </element> </collection> </param> <param name="reads" value="5" /> </conditional> <param name="fasta" value="true" /> <param name="log" value="true" /> <output name="fastaout" ftype="fasta"> <assert_contents> <has_n_lines n="14" /> </assert_contents> </output> <output name="mlst" ftype="txt"> <assert_contents> <has_text_matching expression="4\t14\t16\t24\t8\t14" /> </assert_contents> </output> </test> </tests> <help> **What it does** Search ST number from pubMLST database You can use - Genome assembly (blat search) - Raw data (kma search) **Options:** -i, --identity FLOAT Minimum identity to search gene (default=0.9). -c, --coverage FLOAT Minimum coverage to search gene (default=0.95). -r, --reads INTEGER Minimum reads coverage to search gene (default=10). -f, --fasta FILENAME Write fasta file with gene allele. **License and citation** This Galaxy tool is Copyright © 2022 `B. Valot` and is released under the `GPL3 license`. </help> <citations> </citations> </tool>