Fibronectin Report
Input file:fibronectin_datatest.fasta
Number of sequences in input file:36
Pattern of the sequence bank:AAAGSSVSSVPTKLEVVAATPTSLLISWDA:4-6:V:1:YYRITYGETGGNSPVQEFTVPG:3:TATISGLSPGVDYTITVYA:11-12:PISINYRTGTGGSGGSHHHHHHHHGGSYPYDVPDYAPRVG*GRVGSKDIRAETVESCLAKSHTENSFTNVWKDDKTLDRYANYE
5' restriction site:GCGGCCGC
3' restriction site:GGTACC
Caption:
- Valid sequences that will be part of the next analysis
- Good sequences but will not be part of the next analysis
- Rejected sequences
| Absence of restriction sites | Incorrect number of nucleotides between the restriction sites | Stop codon inside the area of interest | Mutation in the conserved regions | Valid sequences | Amber codon in the sequence (inside the area of interest) |
|---|---|---|---|---|---|
| 1 sequence(s) (2.78%) | 6 sequence(s) (16.67%) | 25 sequence(s) (69.44%) | 0 sequence(s) (0.00%) | 4 sequence(s) (11.11%) | 0 sequence(s) |
| XL2-3_PSEXSEQ-REV_34 | XL1_22_PSEXSEQ-REV_24 XL1_24_PSEXSEQ-REV_26 XL1_30_PSEXSEQ-REV_30 XL1_4_PSEXSEQ-REV_8 XL2-2_PSEXSEQ-REV_33 XL3-4_PSEXSEQ-REV_40 | XL1_10_PSEXSEQ-REV_13 XL1_11_PSEXSEQ-REV_14 XL1_13_PSEXSEQ-REV_16 XL1_14_PSEXSEQ-REV_17 XL1_15_PSEXSEQ-REV_18 XL1_16_PSEXSEQ-REV_19 XL1_17_PSEXSEQ-REV_20 XL1_1_PSEXSEQ-REV_5 XL1_23_PSEXSEQ-REV_25 XL1_25_PSEXSEQ-REV_27 XL1_26_PSEXSEQ-REV_28 XL1_29_PSEXSEQ-REV_29 XL1_33_PSEXSEQ-REV_23 XL1_3_PSEXSEQ-REV_7 XL1_5_PSEXSEQ-REV_9 XL1_6_PSEXSEQ-REV_10 XL1_8_PSEXSEQ-REV_11 XL1_9_PSEXSEQ-REV_12 XL2-1_PSEXSEQ-REV_32 XL2-4_PSEXSEQ-REV_35 XL2-5_PSEXSEQ-REV_36 XL3-1_PSEXSEQ-REV_37 XL3-2_PSEXSEQ-REV_38 XL3-3_PSEXSEQ-REV_39 XL3-5_PSEXSEQ-REV_41 | XL1_12_PSEXSEQ-REV_15 XL1_18_PSEXSEQ-REV_21 XL1_19_PSEXSEQ-REV_22 XL1_2_PSEXSEQ-REV_6 |
The following group of sequences are identical clones on the variable regions:
No clone was found.
Here's the distribution of the repeated sequences in variable regions:
| Variable region | Repeated sequence | Number of occurrences (percentage of valid sequences) |
|---|---|---|
| 3 | YSY | 2 (50.00%) |
The following clusters were generated by MCL:
4 sequences (100.00% of valid sequences)
XL1_12_PSEXSEQ-REV_15
XL1_18_PSEXSEQ-REV_21
XL1_19_PSEXSEQ-REV_22
XL1_2_PSEXSEQ-REV_6
Here's some statistics about the valid sequences:
Mean for the pairwise alignement scores: 25.92
Standard deviation: 3.89
| Pairwise Alignment Score | Number of occurrences |
|---|---|
| 19.00 | 1 |
| 23.80 | 1 |
| 26.30 | 1 |
| 27.30 | 2 |
| 31.80 | 1 |
Valid protein sequences in FASTA format:
Multiple sequence alignment of the valid sequences generated by Clustal Omega:
Protein sequences with an incorrect number of nucleotides between the restriction sites in FASTA format:
Protein sequences with a stop codon in FASTA format: