Mercurial > repos > chawhwa > neuma
view NEUMA-1.2.1/iNIR2iFVKM.pl @ 0:c44c43d185ef draft default tip
NEUMA-1.2.1 Uploaded
author | chawhwa |
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date | Thu, 08 Aug 2013 00:46:13 -0400 |
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#!/usr/bin/perl if(@ARGV<2) { print "usage: $0 iNIR_file iEUMA_file > output.iFVKM\n"; exit; } my ($iNIR_file,$iEUMA_file) = @ARGV; open IN, $iNIR_file or die "Can't open iNIR_file $sample_iNIR_file\n"; <IN>; # skip header while(<IN>){ chomp; split/\t/; $gene = $_[0]."\t".$_[1]; $RNAseq{$gene}=$_[2]; } close IN; open IN, $iEUMA_file or die "Can't open iEUMA file $iEUMA_file\n"; while(<IN>){ chomp; split/\t/; $gene = $_[0]."\t".$_[1]; $iEUMA{$gene}=$_[2]; } close IN; print "gene\tisoform\tiFVK\tread.count\tiEUMA(bp)\n"; for my $gene (sort keys %iEUMA){ next if $iEUMA{$gene} ==0; if(!exists $RNAseq{$gene}){ $RNAseq{$gene} = 0; } my $iFVKM = $RNAseq{$gene} / $iEUMA{$gene} * 1000; print "$gene\t$iFVKM\t$RNAseq{$gene}\t$iEUMA{$gene}\n"; }