0
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1 {
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2 "a_galaxy_workflow": "true",
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3 "annotation": "",
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4 "format-version": "0.1",
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5 "name": "Detrprok_current",
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6 "steps": {
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7 "0": {
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8 "annotation": "Select here annotation file containing coding genes, tRNAs, rRNAs and known ncRNAs. You can download it from GJI (http://genome.jgi.doe.gov) or NCBI. Caution: 1srt column should have exactly the same identifier as the genome sequence used for mapping.",
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9 "id": 0,
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10 "input_connections": {},
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11 "inputs": [
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12 {
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13 "description": "Select here annotation file containing coding genes, tRNAs, rRNAs and known ncRNAs. You can download it from GJI (http://genome.jgi.doe.gov) or NCBI. Caution: 1srt column should have exactly the same identifier as the genome sequence used for mapping.",
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14 "name": "positions list of all genome known annotations"
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15 }
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16 ],
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17 "name": "Input dataset",
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18 "outputs": [],
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19 "position": {
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20 "left": 166,
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21 "top": 364
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22 },
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23 "tool_errors": null,
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24 "tool_id": null,
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25 "tool_state": "{\"name\": \"positions list of all genome known annotations\"}",
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26 "tool_version": null,
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27 "type": "data_input",
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28 "user_outputs": []
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29 },
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30 "1": {
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31 "annotation": "Select here the bam file resulting from the mapping of RNA-seq reads on the genome sequence. You may use Bowtie or another mapper (use appropriate mapping option to retain only uniquely mapping reads).",
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32 "id": 1,
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33 "input_connections": {},
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34 "inputs": [
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35 {
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36 "description": "Select here the bam file resulting from the mapping of RNA-seq reads on the genome sequence. You may use Bowtie or another mapper (use appropriate mapping option to retain only uniquely mapping reads).",
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37 "name": "read alignments on genome"
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38 }
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39 ],
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40 "name": "Input dataset",
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41 "outputs": [],
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42 "position": {
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43 "left": 215,
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44 "top": 484
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45 },
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46 "tool_errors": null,
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47 "tool_id": null,
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48 "tool_state": "{\"name\": \"read alignments on genome\"}",
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49 "tool_version": null,
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50 "type": "data_input",
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51 "user_outputs": []
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52 },
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53 "2": {
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54 "annotation": "",
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55 "id": 2,
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56 "input_connections": {
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57 "inputFile": {
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58 "id": 0,
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59 "output_name": "output"
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60 }
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61 },
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62 "inputs": [],
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63 "name": "clean Gff",
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64 "outputs": [
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65 {
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66 "name": "outputFile",
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67 "type": "gff3"
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68 }
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69 ],
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70 "position": {
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71 "left": 361,
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72 "top": 327
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73 },
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74 "post_job_actions": {},
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75 "tool_errors": null,
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76 "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/cleanGff/1.0.0",
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77 "tool_state": "{\"__page__\": 0, \"type\": \"\\\"tRNA,rRNA,ncRNA,CDS\\\"\", \"inputFile\": \"null\"}",
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78 "tool_version": "1.0.0",
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79 "type": "tool",
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80 "user_outputs": []
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81 },
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82 "3": {
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83 "annotation": "Technical step (format change).",
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84 "id": 3,
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85 "input_connections": {
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86 "input1": {
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87 "id": 1,
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88 "output_name": "output"
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89 }
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90 },
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91 "inputs": [],
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92 "name": "BAM-to-SAM",
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93 "outputs": [
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94 {
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95 "name": "output1",
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96 "type": "sam"
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97 }
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98 ],
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99 "position": {
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100 "left": 418,
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101 "top": 479
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102 },
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103 "post_job_actions": {
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104 "RenameDatasetActionoutput1": {
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105 "action_arguments": {
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106 "newname": "Read alignments in SAM format"
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107 },
|
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108 "action_type": "RenameDatasetAction",
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109 "output_name": "output1"
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110 }
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111 },
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112 "tool_errors": null,
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113 "tool_id": "bam_to_sam",
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114 "tool_state": "{\"header\": \"\\\"False\\\"\", \"input1\": \"null\", \"chromInfo\": \"\\\"/share/apps/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"__page__\": 0}",
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115 "tool_version": "1.0.3",
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116 "type": "tool",
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117 "user_outputs": []
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118 },
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119 "4": {
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120 "annotation": "Clusters annotated proximal genes into operons. \nParameter to be set: op_gap. \nGenes separated by a gap smaller than op_gap are clustered \n(suggested value: 10 to 150).",
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121 "id": 4,
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122 "input_connections": {
|
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123 "formatType|inputFileName": {
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124 "id": 2,
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125 "output_name": "outputFile"
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126 }
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127 },
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128 "inputs": [
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129 {
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130 "description": "runtime parameter for tool Clusterize",
|
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131 "name": "distance"
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132 }
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133 ],
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134 "name": "Clusterize",
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135 "outputs": [
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136 {
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137 "name": "outputFileGff",
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138 "type": "gff3"
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139 },
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140 {
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141 "name": "outputFileLog",
|
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142 "type": "txt"
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143 }
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144 ],
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145 "position": {
|
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146 "left": 547,
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147 "top": 297
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148 },
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149 "post_job_actions": {
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150 "RenameDatasetActionoutputFileGff": {
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151 "action_arguments": {
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152 "newname": "Annotation with \"operon\""
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153 },
|
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154 "action_type": "RenameDatasetAction",
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155 "output_name": "outputFileGff"
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156 }
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157 },
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158 "tool_errors": null,
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159 "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/MergingDataClusterize/1.0.0",
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160 "tool_state": "{\"normalize\": \"\\\"False\\\"\", \"__page__\": 0, \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 3, \\\"FormatInputFileName\\\": \\\"gff3\\\"}\", \"log\": \"\\\"False\\\"\", \"distance\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\", \"colinear\": \"\\\"True\\\"\"}",
|
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161 "tool_version": "1.0.0",
|
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162 "type": "tool",
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163 "user_outputs": []
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164 },
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165 "5": {
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166 "annotation": "Clusters overlapping or proximal reads to produce read clusters. \nParameter to be set: clust_gap. \nReads separated by a gap smaller than \nclust_gap are clustered (suggested value: 0 to 20 ; a value of 0 will cluster only overlapping reads while a value of 20 will cluster reads even if they are 20-nt apart).",
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167 "id": 5,
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168 "input_connections": {
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169 "formatType|inputFileName": {
|
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170 "id": 3,
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171 "output_name": "output1"
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172 }
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173 },
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174 "inputs": [
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175 {
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176 "description": "runtime parameter for tool Clusterize",
|
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177 "name": "distance"
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178 }
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179 ],
|
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180 "name": "Clusterize",
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181 "outputs": [
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182 {
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183 "name": "outputFileGff",
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184 "type": "gff3"
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185 },
|
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186 {
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187 "name": "outputFileLog",
|
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188 "type": "txt"
|
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189 }
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190 ],
|
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191 "position": {
|
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192 "left": 496,
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193 "top": 596
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194 },
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195 "post_job_actions": {
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196 "RenameDatasetActionoutputFileGff": {
|
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197 "action_arguments": {
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198 "newname": "Read clusters"
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199 },
|
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200 "action_type": "RenameDatasetAction",
|
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201 "output_name": "outputFileGff"
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202 }
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203 },
|
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204 "tool_errors": null,
|
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205 "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/MergingDataClusterize/1.0.0",
|
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206 "tool_state": "{\"normalize\": \"\\\"False\\\"\", \"distance\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\", \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 5, \\\"FormatInputFileName\\\": \\\"sam\\\"}\", \"log\": \"\\\"False\\\"\", \"__page__\": 0, \"colinear\": \"\\\"True\\\"\", \"chromInfo\": \"\\\"/share/apps/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\"}",
|
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207 "tool_version": "1.0.0",
|
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208 "type": "tool",
|
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209 "user_outputs": []
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210 },
|
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211 "6": {
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212 "annotation": "Selects read clusters that overlap annotations in the same direction. \nParameter to be set: RNA_gap.\nA read cluster separated from an annotation by a gap smaller than RNA_gap is selected (suggested value: 25).",
|
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213 "id": 6,
|
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214 "input_connections": {
|
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215 "formatType2|inputFileName2": {
|
|
216 "id": 4,
|
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217 "output_name": "outputFileGff"
|
|
218 },
|
|
219 "formatType|inputFileName1": {
|
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220 "id": 5,
|
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221 "output_name": "outputFileGff"
|
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222 }
|
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223 },
|
|
224 "inputs": [
|
|
225 {
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|
226 "description": "runtime parameter for tool Compare Overlapping Small Query",
|
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227 "name": "OptionDistance"
|
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228 }
|
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229 ],
|
|
230 "name": "Compare Overlapping Small Query",
|
|
231 "outputs": [
|
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232 {
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233 "name": "outputFileGff",
|
|
234 "type": "gff3"
|
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235 }
|
|
236 ],
|
|
237 "position": {
|
|
238 "left": 659,
|
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239 "top": 417
|
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240 },
|
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241 "post_job_actions": {
|
|
242 "RenameDatasetActionoutputFileGff": {
|
|
243 "action_arguments": {
|
|
244 "newname": "Read clusters overlapping annotation"
|
|
245 },
|
|
246 "action_type": "RenameDatasetAction",
|
|
247 "output_name": "outputFileGff"
|
|
248 }
|
|
249 },
|
|
250 "tool_errors": null,
|
|
251 "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/CompareOverlappingSmallQuery/1.0.0",
|
|
252 "tool_state": "{\"OptionInclusionQuery\": \"\\\"False\\\"\", \"NotOverlapping\": \"\\\"False\\\"\", \"formatType\": \"{\\\"FormatInputFileName1\\\": \\\"gff3\\\", \\\"inputFileName1\\\": null, \\\"__current_case__\\\": 3}\", \"InvertMatch\": \"\\\"False\\\"\", \"__page__\": 0, \"OptionInclusionRef\": \"\\\"False\\\"\", \"OptionDistance\": \"{\\\"distance\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}, \\\"Dist\\\": \\\"Yes\\\", \\\"__current_case__\\\": 0}\", \"formatType2\": \"{\\\"FormatInputFileName2\\\": \\\"gff\\\", \\\"inputFileName2\\\": null, \\\"__current_case__\\\": 1}\", \"OptionPcOverlapQuery\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionMinOverlap\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionCollinearOrAntiSens\": \"{\\\"__current_case__\\\": 0, \\\"OptionCA\\\": \\\"Collinear\\\"}\", \"OptionPcOverlapRef\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\"}",
|
|
253 "tool_version": "1.0.0",
|
|
254 "type": "tool",
|
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255 "user_outputs": []
|
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256 },
|
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257 "7": {
|
|
258 "annotation": "Selects read clusters that form new independent annotations. \nParameter to be set: RNA_gap.\nRead clusters at a distance from existing annotations larger than RNA_gap are selected. Caution: use same RNA_gap value as in previous step!",
|
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259 "id": 7,
|
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260 "input_connections": {
|
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261 "formatType2|inputFileName2": {
|
|
262 "id": 4,
|
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263 "output_name": "outputFileGff"
|
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264 },
|
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265 "formatType|inputFileName1": {
|
|
266 "id": 5,
|
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267 "output_name": "outputFileGff"
|
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268 }
|
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269 },
|
|
270 "inputs": [
|
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271 {
|
|
272 "description": "runtime parameter for tool Compare Overlapping Small Query",
|
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273 "name": "OptionDistance"
|
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274 }
|
|
275 ],
|
|
276 "name": "Compare Overlapping Small Query",
|
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277 "outputs": [
|
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278 {
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279 "name": "outputFileGff",
|
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280 "type": "gff3"
|
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281 }
|
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282 ],
|
|
283 "position": {
|
|
284 "left": 742,
|
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285 "top": 588
|
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286 },
|
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287 "post_job_actions": {
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288 "RenameDatasetActionoutputFileGff": {
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289 "action_arguments": {
|
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290 "newname": "Read clusters not overlapping annotation"
|
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291 },
|
|
292 "action_type": "RenameDatasetAction",
|
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293 "output_name": "outputFileGff"
|
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294 }
|
|
295 },
|
|
296 "tool_errors": null,
|
|
297 "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/CompareOverlappingSmallQuery/1.0.0",
|
|
298 "tool_state": "{\"OptionInclusionQuery\": \"\\\"False\\\"\", \"NotOverlapping\": \"\\\"False\\\"\", \"formatType\": \"{\\\"FormatInputFileName1\\\": \\\"gff3\\\", \\\"inputFileName1\\\": null, \\\"__current_case__\\\": 3}\", \"InvertMatch\": \"\\\"True\\\"\", \"__page__\": 0, \"OptionInclusionRef\": \"\\\"False\\\"\", \"OptionDistance\": \"{\\\"distance\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}, \\\"Dist\\\": \\\"Yes\\\", \\\"__current_case__\\\": 0}\", \"formatType2\": \"{\\\"FormatInputFileName2\\\": \\\"gff\\\", \\\"inputFileName2\\\": null, \\\"__current_case__\\\": 1}\", \"OptionPcOverlapQuery\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionMinOverlap\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionCollinearOrAntiSens\": \"{\\\"__current_case__\\\": 0, \\\"OptionCA\\\": \\\"Collinear\\\"}\", \"OptionPcOverlapRef\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\"}",
|
|
299 "tool_version": "1.0.0",
|
|
300 "type": "tool",
|
|
301 "user_outputs": []
|
|
302 },
|
|
303 "8": {
|
|
304 "annotation": "Technical step: merging of annotations and their overlapping read clusters. This will be used to extend annotation.",
|
|
305 "id": 8,
|
|
306 "input_connections": {
|
|
307 "input1": {
|
|
308 "id": 4,
|
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309 "output_name": "outputFileGff"
|
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310 },
|
|
311 "queries_0|input2": {
|
|
312 "id": 6,
|
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313 "output_name": "outputFileGff"
|
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314 }
|
|
315 },
|
|
316 "inputs": [],
|
|
317 "name": "Concatenate datasets",
|
|
318 "outputs": [
|
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319 {
|
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320 "name": "out_file1",
|
|
321 "type": "input"
|
|
322 }
|
|
323 ],
|
|
324 "position": {
|
|
325 "left": 819,
|
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326 "top": 294
|
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327 },
|
|
328 "post_job_actions": {
|
|
329 "RenameDatasetActionout_file1": {
|
|
330 "action_arguments": {
|
|
331 "newname": "Annotations and read clusters overlapping them"
|
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332 },
|
|
333 "action_type": "RenameDatasetAction",
|
|
334 "output_name": "out_file1"
|
|
335 }
|
|
336 },
|
|
337 "tool_errors": null,
|
|
338 "tool_id": "cat1",
|
|
339 "tool_state": "{\"__page__\": 0, \"input1\": \"null\", \"chromInfo\": \"\\\"/share/apps/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"queries\": \"[{\\\"input2\\\": null, \\\"__index__\\\": 0}]\"}",
|
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340 "tool_version": "1.0.0",
|
|
341 "type": "tool",
|
|
342 "user_outputs": []
|
|
343 },
|
|
344 "9": {
|
|
345 "annotation": "Extend annotations with overlapping or proximal read clusters in the same direction. \nParameter to be set: RNA_gap.\nRead cluster and annotations separated by a gap smaller than RNA_gap are clustered. Caution: use the same RNA_gap value as previous step!",
|
|
346 "id": 9,
|
|
347 "input_connections": {
|
|
348 "formatType|inputFileName": {
|
|
349 "id": 8,
|
|
350 "output_name": "out_file1"
|
|
351 }
|
|
352 },
|
|
353 "inputs": [
|
|
354 {
|
|
355 "description": "runtime parameter for tool Clusterize",
|
|
356 "name": "distance"
|
|
357 }
|
|
358 ],
|
|
359 "name": "Clusterize",
|
|
360 "outputs": [
|
|
361 {
|
|
362 "name": "outputFileGff",
|
|
363 "type": "gff3"
|
|
364 },
|
|
365 {
|
|
366 "name": "outputFileLog",
|
|
367 "type": "txt"
|
|
368 }
|
|
369 ],
|
|
370 "position": {
|
|
371 "left": 1036,
|
|
372 "top": 307
|
|
373 },
|
|
374 "post_job_actions": {
|
|
375 "RenameDatasetActionoutputFileGff": {
|
|
376 "action_arguments": {
|
|
377 "newname": "Extended annotation"
|
|
378 },
|
|
379 "action_type": "RenameDatasetAction",
|
|
380 "output_name": "outputFileGff"
|
|
381 }
|
|
382 },
|
|
383 "tool_errors": null,
|
|
384 "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/MergingDataClusterize/1.0.0",
|
|
385 "tool_state": "{\"normalize\": \"\\\"False\\\"\", \"distance\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\", \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 3, \\\"FormatInputFileName\\\": \\\"gff3\\\"}\", \"log\": \"\\\"False\\\"\", \"__page__\": 0, \"colinear\": \"\\\"True\\\"\", \"chromInfo\": \"\\\"/share/apps/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\"}",
|
|
386 "tool_version": "1.0.0",
|
|
387 "type": "tool",
|
|
388 "user_outputs": []
|
|
389 },
|
|
390 "10": {
|
|
391 "annotation": "Technical step: suppresses unwanted \"exon\" features created by the use of the distance option in the previous clustering step.",
|
|
392 "id": 10,
|
|
393 "input_connections": {
|
|
394 "input": {
|
|
395 "id": 9,
|
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396 "output_name": "outputFileGff"
|
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397 }
|
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398 },
|
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399 "inputs": [],
|
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400 "name": "Select",
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401 "outputs": [
|
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402 {
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|
403 "name": "out_file1",
|
|
404 "type": "input"
|
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405 }
|
|
406 ],
|
|
407 "position": {
|
|
408 "left": 1232,
|
|
409 "top": 319
|
|
410 },
|
|
411 "post_job_actions": {
|
|
412 "RenameDatasetActionout_file1": {
|
|
413 "action_arguments": {
|
|
414 "newname": "Extended annotation without \"exon\" lines"
|
|
415 },
|
|
416 "action_type": "RenameDatasetAction",
|
|
417 "output_name": "out_file1"
|
|
418 }
|
|
419 },
|
|
420 "tool_errors": null,
|
|
421 "tool_id": "Grep1",
|
|
422 "tool_state": "{\"__page__\": 0, \"input\": \"null\", \"invert\": \"\\\"true\\\"\", \"chromInfo\": \"\\\"/share/apps/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"pattern\": \"\\\"exon\\\"\"}",
|
|
423 "tool_version": "1.0.1",
|
|
424 "type": "tool",
|
|
425 "user_outputs": []
|
|
426 },
|
|
427 "11": {
|
|
428 "annotation": "Technical step: orders extended annotations by positions.",
|
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429 "id": 11,
|
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430 "input_connections": {
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431 "formatType|inputFileName": {
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432 "id": 10,
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433 "output_name": "out_file1"
|
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434 }
|
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435 },
|
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436 "inputs": [],
|
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437 "name": "collapse reads",
|
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438 "outputs": [
|
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439 {
|
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440 "name": "outputFileGff",
|
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441 "type": "gff3"
|
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442 }
|
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443 ],
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444 "position": {
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445 "left": 973,
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446 "top": 460
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447 },
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448 "post_job_actions": {
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449 "RenameDatasetActionoutputFileGff": {
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450 "action_arguments": {
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451 "newname": "Ordrered and extended annotation"
|
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452 },
|
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453 "action_type": "RenameDatasetAction",
|
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454 "output_name": "outputFileGff"
|
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455 }
|
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456 },
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457 "tool_errors": null,
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458 "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/collapseReads/1.0.0",
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459 "tool_state": "{\"__page__\": 0, \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 3, \\\"FormatInputFileName\\\": \\\"gff3\\\"}\", \"chromInfo\": \"\\\"/share/apps/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"strand\": \"\\\"False\\\"\"}",
|
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460 "tool_version": "1.0.0",
|
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461 "type": "tool",
|
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462 "user_outputs": []
|
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463 },
|
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464 "12": {
|
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465 "annotation": "Splits extended annotations into UTRs, CDS and/or operon spacers.",
|
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466 "id": 12,
|
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467 "input_connections": {
|
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468 "referenciesFile": {
|
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469 "id": 4,
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470 "output_name": "outputFileGff"
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471 },
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472 "transcriptsFile": {
|
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473 "id": 11,
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474 "output_name": "outputFileGff"
|
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475 }
|
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476 },
|
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477 "inputs": [],
|
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478 "name": "splitTranscriptGff",
|
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479 "outputs": [
|
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480 {
|
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481 "name": "outputFile",
|
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482 "type": "gff3"
|
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483 }
|
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484 ],
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485 "position": {
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486 "left": 1221,
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487 "top": 456
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488 },
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489 "post_job_actions": {
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490 "RenameDatasetActionoutputFile": {
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491 "action_arguments": {
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492 "newname": "UTRs, CDSs and/or operons"
|
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493 },
|
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494 "action_type": "RenameDatasetAction",
|
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495 "output_name": "outputFile"
|
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496 }
|
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497 },
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498 "tool_errors": null,
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499 "tool_id": "testtoolshed.g2.bx.psu.edu/repos/clairetn/detrprok_scripts/splitTranscriptGff/1.0.0",
|
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500 "tool_state": "{\"transcriptsFile\": \"null\", \"__page__\": 0, \"referenciesFile\": \"null\"}",
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501 "tool_version": "1.0.0",
|
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502 "type": "tool",
|
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503 "user_outputs": []
|
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504 },
|
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505 "13": {
|
|
506 "annotation": "Selection of read clusters that are antisense of an extended annotation. \nThis step produces a first list of antisense candidates.",
|
|
507 "id": 13,
|
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508 "input_connections": {
|
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509 "formatType2|inputFileName2": {
|
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510 "id": 11,
|
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511 "output_name": "outputFileGff"
|
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512 },
|
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513 "formatType|inputFileName1": {
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514 "id": 7,
|
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515 "output_name": "outputFileGff"
|
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516 }
|
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517 },
|
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518 "inputs": [],
|
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519 "name": "Compare Overlapping Small Query",
|
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520 "outputs": [
|
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521 {
|
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522 "name": "outputFileGff",
|
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523 "type": "gff3"
|
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524 }
|
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525 ],
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526 "position": {
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527 "left": 1155,
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528 "top": 617
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529 },
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530 "post_job_actions": {
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531 "RenameDatasetActionoutputFileGff": {
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532 "action_arguments": {
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533 "newname": "first list of asRNA"
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534 },
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535 "action_type": "RenameDatasetAction",
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536 "output_name": "outputFileGff"
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537 }
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538 },
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539 "tool_errors": null,
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540 "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/CompareOverlappingSmallQuery/1.0.0",
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541 "tool_state": "{\"OptionInclusionQuery\": \"\\\"True\\\"\", \"NotOverlapping\": \"\\\"False\\\"\", \"formatType\": \"{\\\"FormatInputFileName1\\\": \\\"gff3\\\", \\\"inputFileName1\\\": null, \\\"__current_case__\\\": 3}\", \"InvertMatch\": \"\\\"False\\\"\", \"__page__\": 0, \"OptionInclusionRef\": \"\\\"False\\\"\", \"OptionDistance\": \"{\\\"Dist\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"formatType2\": \"{\\\"FormatInputFileName2\\\": \\\"gff3\\\", \\\"inputFileName2\\\": null, \\\"__current_case__\\\": 3}\", \"OptionPcOverlapQuery\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionMinOverlap\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionCollinearOrAntiSens\": \"{\\\"__current_case__\\\": 1, \\\"OptionCA\\\": \\\"AntiSens\\\"}\", \"OptionPcOverlapRef\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\"}",
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542 "tool_version": "1.0.0",
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543 "type": "tool",
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544 "user_outputs": []
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545 },
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546 "14": {
|
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547 "annotation": "Selection of read clusters that are outside and not in antisense of an extended annotation.\nThis step produces a first list of small RNAs.",
|
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548 "id": 14,
|
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549 "input_connections": {
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550 "formatType2|inputFileName2": {
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551 "id": 11,
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552 "output_name": "outputFileGff"
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553 },
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554 "formatType|inputFileName1": {
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555 "id": 7,
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556 "output_name": "outputFileGff"
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557 }
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558 },
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559 "inputs": [],
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560 "name": "Compare Overlapping Small Query",
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561 "outputs": [
|
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562 {
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563 "name": "outputFileGff",
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564 "type": "gff3"
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565 }
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566 ],
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567 "position": {
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568 "left": 1068,
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569 "top": 766
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570 },
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571 "post_job_actions": {
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572 "RenameDatasetActionoutputFileGff": {
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573 "action_arguments": {
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574 "newname": "first list of sRNA"
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575 },
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576 "action_type": "RenameDatasetAction",
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577 "output_name": "outputFileGff"
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578 }
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579 },
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580 "tool_errors": null,
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581 "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/CompareOverlappingSmallQuery/1.0.0",
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582 "tool_state": "{\"OptionInclusionQuery\": \"\\\"True\\\"\", \"NotOverlapping\": \"\\\"False\\\"\", \"formatType\": \"{\\\"FormatInputFileName1\\\": \\\"gff3\\\", \\\"inputFileName1\\\": null, \\\"__current_case__\\\": 3}\", \"InvertMatch\": \"\\\"True\\\"\", \"__page__\": 0, \"OptionInclusionRef\": \"\\\"False\\\"\", \"OptionDistance\": \"{\\\"Dist\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"formatType2\": \"{\\\"FormatInputFileName2\\\": \\\"gff3\\\", \\\"inputFileName2\\\": null, \\\"__current_case__\\\": 3}\", \"OptionPcOverlapQuery\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionMinOverlap\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionCollinearOrAntiSens\": \"{\\\"__current_case__\\\": 1, \\\"OptionCA\\\": \\\"AntiSens\\\"}\", \"OptionPcOverlapRef\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\"}",
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583 "tool_version": "1.0.0",
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584 "type": "tool",
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585 "user_outputs": []
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586 },
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587 "15": {
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588 "annotation": "Counts reads overlapping UTRs, CDSs and/or operon spacers in order to compute read coverage (with a minimal overlapping region of 10 nt between read and genetic element). Output file of this step can be used to obtain read coverage of 5'UTRs, 3'UTRs, CDSs, or operon spacers.",
|
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589 "id": 15,
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590 "input_connections": {
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591 "formatType2|inputFileName2": {
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592 "id": 3,
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593 "output_name": "output1"
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594 },
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595 "formatType|inputFileName1": {
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596 "id": 12,
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597 "output_name": "outputFile"
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598 }
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599 },
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600 "inputs": [],
|
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601 "name": "Compare Overlapping Small Reference",
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602 "outputs": [
|
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603 {
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604 "name": "outputFileGff",
|
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605 "type": "gff3"
|
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606 }
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607 ],
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608 "position": {
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609 "left": 1417,
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610 "top": 506
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611 },
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612 "post_job_actions": {
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613 "RenameDatasetActionoutputFileGff": {
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614 "action_arguments": {
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615 "newname": "UTRs, CDSs and operons (with the number of overlapping reads)"
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616 },
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617 "action_type": "RenameDatasetAction",
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618 "output_name": "outputFileGff"
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619 }
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620 },
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621 "tool_errors": null,
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622 "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/CompareOverlappingSmallRef/1.0.0",
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623 "tool_state": "{\"OptionInclusionQuery\": \"\\\"False\\\"\", \"NotOverlapping\": \"\\\"False\\\"\", \"formatType\": \"{\\\"FormatInputFileName1\\\": \\\"gff3\\\", \\\"inputFileName1\\\": null, \\\"__current_case__\\\": 3}\", \"InvertMatch\": \"\\\"False\\\"\", \"__page__\": 0, \"OptionInclusionRef\": \"\\\"False\\\"\", \"OptionDistance\": \"{\\\"Dist\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"formatType2\": \"{\\\"FormatInputFileName2\\\": \\\"sam\\\", \\\"inputFileName2\\\": null, \\\"__current_case__\\\": 4}\", \"OptionPcOverlapQuery\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionMinOverlap\": \"{\\\"present\\\": \\\"Yes\\\", \\\"__current_case__\\\": 0, \\\"minOverlap\\\": \\\"10\\\"}\", \"OptionCollinearOrAntiSens\": \"{\\\"__current_case__\\\": 0, \\\"OptionCA\\\": \\\"Collinear\\\"}\", \"OptionPcOverlapRef\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\"}",
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624 "tool_version": "1.0.0",
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625 "type": "tool",
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626 "user_outputs": []
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627 },
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628 "16": {
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629 "annotation": "Filters antisense RNA candidates based on their number of overlapping reads.\nParameter to be set: as_min_reads.\nAntisense RNAs with a number of reads higher than as_min_reads are selected (suggested value: 22).",
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630 "id": 16,
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631 "input_connections": {
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632 "formatType|inputFileName": {
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633 "id": 13,
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634 "output_name": "outputFileGff"
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635 }
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636 },
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637 "inputs": [
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638 {
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639 "description": "runtime parameter for tool select by tag",
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640 "name": "OptionMin"
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641 }
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642 ],
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643 "name": "select by tag",
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644 "outputs": [
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645 {
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646 "name": "outputFileGff",
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647 "type": "gff3"
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648 }
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649 ],
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650 "position": {
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651 "left": 1461,
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652 "top": 663
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653 },
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654 "post_job_actions": {
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655 "RenameDatasetActionoutputFileGff": {
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656 "action_arguments": {
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657 "newname": "asRNA list modified (filtered on their number of overlapping reads)"
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658 },
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659 "action_type": "RenameDatasetAction",
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660 "output_name": "outputFileGff"
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661 }
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662 },
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663 "tool_errors": null,
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664 "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/SelectByTag/1.0.0",
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665 "tool_state": "{\"__page__\": 0, \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 1, \\\"FormatInputFileName\\\": \\\"gff\\\"}\", \"OptionMin\": \"{\\\"minimum\\\": \\\"Yes\\\", \\\"__current_case__\\\": 0, \\\"min\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}}\", \"Tag\": \"\\\"nbElements\\\"\", \"OptionMax\": \"{\\\"maximum\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionValue\": \"{\\\"Value\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"chromInfo\": \"\\\"/share/apps/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"OptionDefault\": \"{\\\"default\\\": \\\"Yes\\\", \\\"defaultValue\\\": \\\"0.0\\\", \\\"__current_case__\\\": 0}\"}",
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666 "tool_version": "1.0.0",
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667 "type": "tool",
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668 "user_outputs": []
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669 },
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670 "17": {
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671 "annotation": "Filters sRNA candidates based on their number of overlapping reads.\nParameter to be set: sRNA_min_reads.\nSmall RNAs with a number of reads higher than sRNA_min_reads are selected (suggested value: 12).",
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672 "id": 17,
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673 "input_connections": {
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674 "formatType|inputFileName": {
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675 "id": 14,
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676 "output_name": "outputFileGff"
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677 }
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678 },
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679 "inputs": [
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680 {
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681 "description": "runtime parameter for tool select by tag",
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682 "name": "OptionMin"
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683 }
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684 ],
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685 "name": "select by tag",
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686 "outputs": [
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687 {
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688 "name": "outputFileGff",
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689 "type": "gff3"
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690 }
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691 ],
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692 "position": {
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693 "left": 1389,
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694 "top": 794
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695 },
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696 "post_job_actions": {
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697 "RenameDatasetActionoutputFileGff": {
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698 "action_arguments": {
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699 "newname": "sRNA list modified (filtered on their number of overlapping reads)"
|
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700 },
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701 "action_type": "RenameDatasetAction",
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702 "output_name": "outputFileGff"
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703 }
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704 },
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705 "tool_errors": null,
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706 "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/SelectByTag/1.0.0",
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707 "tool_state": "{\"__page__\": 0, \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 3, \\\"FormatInputFileName\\\": \\\"gff3\\\"}\", \"OptionMin\": \"{\\\"minimum\\\": \\\"Yes\\\", \\\"__current_case__\\\": 0, \\\"min\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}}\", \"Tag\": \"\\\"nbElements\\\"\", \"OptionMax\": \"{\\\"maximum\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionValue\": \"{\\\"Value\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"chromInfo\": \"\\\"/share/apps/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"OptionDefault\": \"{\\\"default\\\": \\\"Yes\\\", \\\"defaultValue\\\": \\\"0.0\\\", \\\"__current_case__\\\": 0}\"}",
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708 "tool_version": "1.0.0",
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709 "type": "tool",
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710 "user_outputs": []
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711 },
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712 "18": {
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713 "annotation": "Technical step: coverage computation needs \"nbElements\" tag in place of \"nbOverlaps\".",
|
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714 "id": 18,
|
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715 "input_connections": {
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716 "formatType|inputFileName": {
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717 "id": 15,
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718 "output_name": "outputFileGff"
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719 }
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720 },
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721 "inputs": [],
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722 "name": "change tag name",
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723 "outputs": [
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724 {
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725 "name": "outputFileGff",
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726 "type": "gff3"
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727 }
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728 ],
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729 "position": {
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730 "left": 1696,
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731 "top": 541
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732 },
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733 "post_job_actions": {
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734 "RenameDatasetActionoutputFileGff": {
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735 "action_arguments": {
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736 "newname": "long 5'UTR list modified (tag \"nbElements\")"
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737 },
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738 "action_type": "RenameDatasetAction",
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739 "output_name": "outputFileGff"
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740 }
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741 },
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744 "tool_state": "{\"__page__\": 0, \"Tag\": \"\\\"nbOverlaps\\\"\", \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 3, \\\"FormatInputFileName\\\": \\\"gff3\\\"}\", \"name\": \"\\\"nbElements\\\"\"}",
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745 "tool_version": "1.0.0",
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746 "type": "tool",
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747 "user_outputs": []
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748 },
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749 "19": {
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750 "annotation": "Clusters proximal antisense RNAs in the same direction.\nParameter to be set: RNA_merge.\nAntisense RNAs located less than RNA_merge apart are merged (suggestion: 50).",
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751 "id": 19,
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752 "input_connections": {
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753 "formatType|inputFileName": {
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754 "id": 16,
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755 "output_name": "outputFileGff"
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756 }
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757 },
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758 "inputs": [
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759 {
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760 "description": "runtime parameter for tool Clusterize",
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761 "name": "distance"
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762 }
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763 ],
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764 "name": "Clusterize",
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765 "outputs": [
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766 {
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767 "name": "outputFileGff",
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768 "type": "gff3"
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769 },
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770 {
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771 "name": "outputFileLog",
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772 "type": "txt"
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773 }
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774 ],
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775 "position": {
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776 "left": 1658,
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777 "top": 660
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778 },
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779 "post_job_actions": {
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780 "RenameDatasetActionoutputFileGff": {
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781 "action_arguments": {
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782 "newname": "asRNA list modified (asRNA clusters)"
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783 },
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784 "action_type": "RenameDatasetAction",
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785 "output_name": "outputFileGff"
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786 }
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787 },
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788 "tool_errors": null,
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789 "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/MergingDataClusterize/1.0.0",
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790 "tool_state": "{\"normalize\": \"\\\"False\\\"\", \"__page__\": 0, \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 3, \\\"FormatInputFileName\\\": \\\"gff3\\\"}\", \"log\": \"\\\"False\\\"\", \"distance\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\", \"colinear\": \"\\\"True\\\"\"}",
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791 "tool_version": "1.0.0",
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792 "type": "tool",
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793 "user_outputs": []
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794 },
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795 "20": {
|
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796 "annotation": "Clusters proximal small RNAs in the same direction.\nParameter to be set: RNA_merge.\nSmall RNAs located less than RNA_merge apart are merged (suggestion: 50).",
|
|
797 "id": 20,
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798 "input_connections": {
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799 "formatType|inputFileName": {
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800 "id": 17,
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801 "output_name": "outputFileGff"
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802 }
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803 },
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804 "inputs": [],
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805 "name": "Clusterize",
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806 "outputs": [
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807 {
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808 "name": "outputFileGff",
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809 "type": "gff3"
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810 },
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811 {
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812 "name": "outputFileLog",
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813 "type": "txt"
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814 }
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815 ],
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816 "position": {
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817 "left": 1602,
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818 "top": 797
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819 },
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820 "post_job_actions": {
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821 "RenameDatasetActionoutputFileGff": {
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