Mercurial > repos > cpt > cpt_intron_detection
diff cpt_intron_detect/intron_detection.xml @ 0:1a19092729be draft
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author | cpt |
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date | Fri, 13 May 2022 05:08:54 +0000 |
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children | e0e294a1ccdd |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/cpt_intron_detect/intron_detection.xml Fri May 13 05:08:54 2022 +0000 @@ -0,0 +1,57 @@ +<?xml version="1.1"?> +<tool id="edu.tamu.cpt2.phage.intron_detection" name="Interrupted gene detection tool" version="19.1.0.0"> + <description>based on nearby protein blast results</description> + <macros> + <import>macros.xml</import> + <import>cpt-macros.xml</import> + </macros> + <requirements> + <requirement type="package" version="3.6">python</requirement> + <requirement type="package" version="1.77">biopython</requirement> + <requirement type="package" version="1.1.3">cpt_gffparser</requirement> + </requirements> + <command detect_errors="aggressive"><![CDATA[ +python $__tool_directory__/intron_detection.py + +"$gff3_data" +"$blast" +--minimum $minimum +--maximum $maximum +--idThresh $idThresh +> $output]]></command> + <inputs> + <expand macro="gff3_input" /> + <param label="Blastp Results" name="blast" type="data" format="blastxml"/> + <param label="Separation Minimum" name="minimum" type="text" value="-1" + help="Minimum number of amino acids a CDS pair must be separated by to detect an intron. Set to a negative number to instead allow CDS to overlap by up to that many AAs."/> + <param label="Separation Maximum" name="maximum" type="integer" value="10000" + help="Maximum number of bases a CDS pair may be separated by to detect an intron (eg, distance from end of 1 cds to the start of another)."/> + <param label="Percent Identity Threshold" name="idThresh" type="float" value=".30" + help="Minimum Identity Percentage for HSP to be considered for intron comparison (0.00 to 1.00)"/> + </inputs> + <outputs> + <data format="gff3" name="output" label="Potential interrupted genes"/> + </outputs> + <tests> + <test> + <param name="gff3_data" value="T7_CLEAN.gff3"/> + <param name="blast" value="T7_NR.blastxml"/> + <param name="minimum" value="10" /> + <param name="maximum" value="2000" /> + <param name="idThresh" value="0.3" /> + <output name="output" file="T7_INTRON.gff3"/> + </test> + </tests> + <help><![CDATA[ +**What it does** + +This tool parses BLAST XML results and returns potential intron regions by +identifying query proteins with BLAST hits to the same target protein within +the maximum number of bases set above. This distance will also wraparound the +ends of the genome. The list of hits is returned as a gff3. + +(Tool formerly named Intron Detection, updated Nov 2020) + + ]]></help> + <expand macro="citations-2020" /> +</tool>