Mercurial > repos > cpt > cpt_psm_plotter
annotate cpt_genbank2circosk.pl @ 1:8691c1c61a8e draft default tip
planemo upload commit 94b0cd1fff0826c6db3e7dc0c91c0c5a8be8bb0c
author | cpt |
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date | Mon, 05 Jun 2023 02:48:47 +0000 |
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1 #!/usr/bin/perl |
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2 # |
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3 # Code written by Eric Rasche |
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4 # mailto:rasche.eric@yandex.ru |
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5 # tel:404.692.2048 |
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6 # http://eric.rasche.co.uk |
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7 # for |
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8 # Center for Phage Technology |
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9 # |
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10 |
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11 use strict; |
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12 use warnings; |
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13 |
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14 use CPT::GalaxyGetOpt; |
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15 use Data::Dumper; |
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16 |
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17 my $ggo = CPT::GalaxyGetOpt->new(); |
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18 my %colors = map { $_ => $_ } qw(red orange yellow green blue gray black white); |
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19 my %intensity = map { $_ => $_ } qw (vvvvl vvvl vvl vl vd vvd vvvd vvvvd); |
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20 |
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21 my $options = $ggo->getOptions( |
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22 'options' => [ |
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23 [ |
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24 'file', |
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25 'Input file', |
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26 { |
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27 required => 1, |
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28 validate => 'File/Input', |
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29 file_format => ['genbank', 'embl', 'txt'], |
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30 } |
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31 ], |
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32 [ |
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33 'chromosome' => 'Name for the |
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34 chromosome inside Circos', |
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35 { |
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36 required => 1, |
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37 validate => 'String' |
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38 } |
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39 ], |
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40 [ |
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41 'color' => 'Color to use for Circos plot', |
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42 { |
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43 required => 1, |
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44 validate => 'Option', |
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45 options => \%colors, |
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46 } |
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47 ], |
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48 [ |
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49 'intensity' => 'Circos color intensity. ', |
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50 { |
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51 validate => 'Option', |
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52 options => \%intensity, |
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53 } |
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54 ], |
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55 ], |
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56 'outputs' => [ |
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57 [ |
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58 'circosk', |
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59 'Circos Karyotype File', |
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60 { |
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61 validate => 'File/Output', |
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62 required => 1, |
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63 default => 'karyotype', |
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64 data_format => 'text/plain', |
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65 default_format => 'TXT' |
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66 } |
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67 ], |
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68 ], |
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69 'defaults' => [ |
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70 'appname' => 'Genbank2CircosK', |
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71 'appid' => 'Genbank2CircosK', |
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72 'appvers' => '1.94', |
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73 'appdesc' => |
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74 'Convert genbank files to Circos Karyotype configuration files', |
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75 ], |
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76 'tests' => [ |
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77 { |
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78 test_name => "Default", |
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79 params => { |
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80 'file' => 'test-data/inputs/multi.gbk', |
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81 'chromosome' => 'test', |
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82 'color' => 'red', |
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83 'intensity' => 'vvvl', |
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84 }, |
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85 outputs => { |
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86 'circosk' => ['karyotype.txt', 'test-data/outputs/circosk.conf'], |
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87 } |
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88 }, |
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89 ], |
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90 ); |
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91 |
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92 use CPT::Bio; |
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93 my $bio = CPT::Bio->new(); |
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94 |
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95 my @results; |
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96 my $c = 0; |
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97 my $seqio = $bio->requestCopyIO( file => $options->{file} ); |
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98 |
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99 while(my $seqobj = $seqio->next_seq()){ |
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100 foreach my $feat ( $seqobj->get_SeqFeatures () ) { |
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101 |
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102 #band test 12 CDS__test_1gbk 5715 6335 red] |
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103 next if ( $feat->primary_tag ne 'CDS' ); |
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104 my $id = $bio->_getIdentifier($feat); |
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105 $id =~ s/\s+/_/g; |
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106 push( |
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107 @results, |
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108 join( |
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109 ' ', |
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110 ( |
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111 'band', |
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112 $options->{'chromosome'}, |
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113 $c++, |
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114 $id, |
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115 $feat->start, |
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116 $feat->end, |
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117 (defined $options->{'intensity'} ? $options->{'intensity'} : '') . $options->{'color'} |
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118 ) |
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119 ) |
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120 ); |
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121 } |
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122 } |
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123 |
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124 my $z = join( "\n", @results ); |
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125 |
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126 use CPT::OutputFiles; |
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127 my $output = CPT::OutputFiles->new( |
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128 name => 'circosk', |
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129 GGO => $ggo, |
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130 ); |
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131 $output->CRR(data => $z); |