Mercurial > repos > cpt > cpt_psm_recombine
annotate PSM_Recombine.py @ 2:d09346afadfa draft default tip
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author | cpt |
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date | Fri, 05 Jan 2024 05:56:18 +0000 |
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1 #!/usr/bin/env python |
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2 import argparse |
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3 import logging |
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4 from Bio import SeqIO |
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5 |
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6 logging.basicConfig(level=logging.INFO) |
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7 log = logging.getLogger(__name__) |
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8 |
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9 |
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10 if __name__ == "__main__": |
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11 parser = argparse.ArgumentParser(description="Identify shine-dalgarno sequences") |
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12 parser.add_argument("psmTable", type=argparse.FileType("r")) |
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13 parser.add_argument("gbkList", type=argparse.FileType("r"), nargs="+") |
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14 args = parser.parse_args() |
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15 |
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16 gbkRecs = [] |
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17 recIDs = [] |
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18 recFlatten = [] # Can only seek argparse file once |
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19 |
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20 for f in args.gbkList: |
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21 tempRecs = SeqIO.parse(f, "genbank") |
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22 for rec in tempRecs: |
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23 recFlatten.append(rec) |
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24 |
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25 for line in args.psmTable: |
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26 lineElems = line.split("\t") |
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27 numGenes = 0 |
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28 accession = "" |
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29 lineOut = "" |
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30 if recIDs == []: |
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31 for i in lineElems: |
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32 recIDs.append(i.strip()) |
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33 lineOut += i.strip() + "\t" |
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34 for rec in recFlatten: |
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35 if i.strip() in rec.id or rec.id in i.strip(): |
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36 gbkRecs.append(rec) |
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37 lineOut += "No. of phages in which gene is present\tBest Database Match" |
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38 print(lineOut) |
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39 continue |
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40 |
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41 for i in range(0, len(lineElems)): |
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42 checkFeat = lineElems[i].strip() |
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43 if checkFeat == "-": |
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44 lineOut += "(-)\t" |
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45 continue |
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46 else: |
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47 lineOut += checkFeat + "\t" |
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48 numGenes += 1 |
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49 if accession == "": |
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50 for feat in gbkRecs[i].features: |
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51 if ( |
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52 "locus_tag" in feat.qualifiers.keys() |
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53 and feat.qualifiers["locus_tag"][0] == checkFeat |
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54 ): |
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55 if "protein_id" in feat.qualifiers.keys(): |
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56 accession = feat.qualifiers["protein_id"][0] |
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57 break # Comment out if we need to get more info |
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58 lineOut += str(numGenes) + "\t" + accession |
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59 print(lineOut) |