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view lib/CPT/Bio/RBS/Algo/Naive.pm @ 1:97ef96676b48 draft
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author | cpt |
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date | Mon, 05 Jun 2023 02:51:26 +0000 |
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package CPT::Bio::RBS::Algo::Naive; use Moose; with 'CPT::Bio::RBS::Algo'; use CPT::Bio::RBS_Object; my @SDs = ( 'aggaggt', 'ggaggt', 'aggagg', 'aggag', 'gaggt', 'ggagg', 'aggt', 'gggt', 'gagg', 'gggg', 'agga', 'ggag', 'gga', 'gag', 'agg', 'ggt', ); sub predict { my ( $self, %params ) = @_; my $upstream = $params{sequence}; my $only_best = $params{return_best}; my $length = length($upstream); my @results = (); foreach my $rbs ( @SDs ){ while ($upstream =~ /$rbs/g) { # Position of regex match my $loc = $-[0]; # Seq before RBS my $before = substr($upstream,0, $loc); # Seq after RBS my $after = substr($upstream, $loc + length($rbs)); my $rbs_o = CPT::Bio::RBS_Object->new( upstream => sprintf('%s %s %s', $before , uc($rbs) , $after), score => $self->score_match($rbs, length($after)), rbs_seq => uc($rbs), separation => length($after), ); push( @results, $rbs_o ); } } @results = sort { $b->score() <=> $a->score() } @results; if (@results) { if($only_best){ return ($results[0]); }else{ return @results; } } else { return ( CPT::Bio::RBS_Object->new( upstream =>$upstream, score => '-1', rbs_seq => 'None', separation => -1, ) ); } } sub score_match { my ($self, $match, $dist) = @_; return length($match); } 1; __END__ =pod =encoding UTF-8 =head1 NAME CPT::Bio::RBS::Algo::Naive =head1 VERSION version 1.99.4 =head1 AUTHOR Eric Rasche <rasche.eric@yandex.ru> =head1 COPYRIGHT AND LICENSE This software is Copyright (c) 2014 by Eric Rasche. This is free software, licensed under: The GNU General Public License, Version 3, June 2007 =cut