Mercurial > repos > crs4 > glimmer
diff extract_wrapper.xml @ 0:9c8ffce71f7c draft default tip
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author | crs4 |
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date | Mon, 09 Sep 2013 12:16:17 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/extract_wrapper.xml Mon Sep 09 12:16:17 2013 -0400 @@ -0,0 +1,80 @@ +<tool id="extract_wrapper" name="Extract" version="0.2"> + <description></description> + <requirements> + <requirement type="package" version="3.02">glimmer</requirement> + </requirements> + <command interpreter="python"> + extract_wrapper.py --exSequence $exSequence --exCoords $exCoords + #if $ex2Fields + --ex2Fields + #end if + #if $exDir + --exDir + #end if + #if str($exMinLen) + --exMinLen=$exMinLen + #end if + #if $exNoStart + --exNoStart + #end if + #if $exNoStop + --exNoStop + #end if + #if $exNoWrap + --exNoWrap + #end if + --exOutput $exOutput --logfile $logfile + </command> + + <inputs> + <param name="exSequence" type="data" format="fasta" label="DNA sequence to be analyzed" help="FASTA format" /> + + <param name="exCoords" type="data" format="glimmer_coords" label="Coordinates produced by Long-ORFs" /> + + <param name="ex2Fields" type="boolean" checked="false" label="Output sequence in 2 fields (-2, --2_fields)" help="Output each sequence as 2 fields (tag and sequence) on a single line." /> + + <param name="exDir" type="boolean" checked="false" label="Specify sequence direction (-d, --dir)" help="Use the 4th column of each input line to specify the direction of the sequence. Positive is forward, negative is reverse. The input sequence is assumed to be circular." /> + + <param name="exMinLen" type="integer" value="" optional="true" label="Minimum length of sequence (-l, --minlen)" help="Don't output any sequence shorter than n characters." /> + + <param name="exNoStart" type="boolean" checked="false" label="Omit first characters (-s, --nostart)" help="Omit the first 3 characters of each output string." /> + + <param name="exNoStop" type="boolean" checked="false" label="Omit last characters (-t, --nostop)" help="Omit the last 3 characters of each output string." /> + + <param name="exNoWrap" type="boolean" checked="false" label="No wraparound (-w, --nowrap)" help="Use the actual input coordinates without any wraparound that would be needed by a circular genome. Without this option, the output sequence is the shorter of the two ways around the circle. Tick 'Specify sequence direction' to specify direction explicitly." /> + </inputs> + + <outputs> + <data name="logfile" format="txt" label="${tool.name} on ${on_string}: log" /> + <data name="exOutput" format="fasta" label="${tool.name} on ${on_string}: output" /> + </outputs> + + <tests> + + </tests> + <help> +**What it does** + +Read FASTA-format *sequence-file* and extract from it the subsequences specified by *coords*. By default, *coords* is the name of a file containing lines of the form *tag* *start* *stop* [*frame*] ... +Coordinates are inclusive counting from 1, e.g. *1 3* represents the 1st 3 characters of the sequence. For each line the corresponding region of *sequence-file* is extracted and output (after reverse-complementing if necessary). + + +**License and citation** + +This Galaxy tool is Copyright © 2013 `CRS4 Srl.`_ and is released under the `MIT license`_. + +.. _CRS4 Srl.: http://www.crs4.it/ +.. _MIT license: http://opensource.org/licenses/MIT + +If you use this tool in Galaxy, please cite |Cuccuru2013|_. + +.. |Cuccuru2013| replace:: Cuccuru, G., Orsini, M., Pinna, A., Sbardellati, A., Soranzo, N., Travaglione, A., Uva, P., Zanetti, G., Fotia, G. (2013) Orione, a web-based framework for NGS analysis in microbiology. *Submitted* +.. _Cuccuru2013: http://orione.crs4.it/ + +This tool uses `Glimmer`_, which is licensed separately. Please cite |Delcher2007|_. + +.. _Glimmer: http://ccb.jhu.edu/software/glimmer/index.shtml +.. |Delcher2007| replace:: Delcher, A. L., Bratke, K. A., Powers, E. C., Salzberg, S. L. (2007) Identifying bacterial genes and endosymbiont DNA with Glimmer. *Bioinformatics* 23(6), 673-679 +.. _Delcher2007: http://bioinformatics.oxfordjournals.org/content/23/6/673 + </help> +</tool>