# HG changeset patch # User crs4 # Date 1415298015 18000 # Node ID 1fea05cdc1a1b1bf73128b85cc33739358793875 # Parent 6adb6e16b00fb12966f1e012e1612d356ca021d4 Update Orione citation. Upgrade Mosaik dependency to v. 2.2.28 (2.2.30 is buggy, see https://github.com/wanpinglee/MOSAIK/issues/11 ). Use 2.1.78 neural networks. Add package_zlib_1_2_8 and package_samtools_0_1_19 dependencies. Add . diff -r 6adb6e16b00f -r 1fea05cdc1a1 mosaik2.xml --- a/mosaik2.xml Wed Apr 02 12:32:52 2014 -0400 +++ b/mosaik2.xml Thu Nov 06 13:20:15 2014 -0500 @@ -1,8 +1,8 @@ - + - mosaik + mosaik samtools MosaikJump | sed -e 's/\x1b\[[[:digit:]]\{1,2\}\(;[[:digit:]]\{1,2\}\)\{0,1\}m//g' | sed -n 's/MosaikJump \([[:digit:].]\{1,\}\).*/\1/p' @@ -23,7 +23,7 @@ #set $ls_string = '-ls %s' % $paired.ls #end if -st $st -out mosaik_reads_file; - MosaikAligner -p \${GALAXY_SLOTS:-4} -ia mosaik_ref_file -in mosaik_reads_file -out mosaik_aligned_file -annpe \$MOSAIK_NETWORK_DIR/2.1.26.pe.100.0065.ann -annse \$MOSAIK_NETWORK_DIR/2.1.26.se.100.005.ann $ls_string + MosaikAligner -p \${GALAXY_SLOTS:-4} -ia mosaik_ref_file -in mosaik_reads_file -out mosaik_aligned_file -annpe \$MOSAIK_NETWORK_DIR/2.1.78.pe.ann -annse \$MOSAIK_NETWORK_DIR/2.1.78.se.ann $ls_string #if str($mm) -mm $mm #end if @@ -36,7 +36,7 @@ #if str($mhp) -mhp $mhp #end if - -hs 15; + -hs 15 && #if str($outFormat) == 'bam' samtools sort -o mosaik_aligned_file.bam out_prefix > $output #else @@ -141,15 +141,22 @@ .. _CRS4 Srl.: http://www.crs4.it/ .. _Academic Free License v. 3.0: http://opensource.org/licenses/AFL-3.0 -If you use this tool in Galaxy, please cite |Cuccuru2014|_. - -.. |Cuccuru2014| replace:: Cuccuru, G., Orsini, M., Pinna, A., Sbardellati, A., Soranzo, N., Travaglione, A., Uva, P., Zanetti, G., Fotia, G. (2014) Orione, a web-based framework for NGS analysis in microbiology. *Bioinformatics*, accepted -.. _Cuccuru2014: http://bioinformatics.oxfordjournals.org/content/early/2014/03/10/bioinformatics.btu135 - -This tool uses `MOSAIK`_, which is licensed separately. Please cite |Lee2014|_. +You can use this tool only if you agree to the license terms of: `MOSAIK`_. .. _MOSAIK: https://code.google.com/p/mosaik-aligner/ + +If you use this tool, please cite: + +- |Cuccuru2014|_ +- |Lee2014|_. + +.. |Cuccuru2014| replace:: Cuccuru, G., Orsini, M., Pinna, A., Sbardellati, A., Soranzo, N., Travaglione, A., Uva, P., Zanetti, G., Fotia, G. (2014) Orione, a web-based framework for NGS analysis in microbiology. *Bioinformatics* 30(13), 1928-1929 +.. _Cuccuru2014: http://bioinformatics.oxfordjournals.org/content/30/13/1928 .. |Lee2014| replace:: Lee, W.-P., *et al.* (2014) MOSAIK: A Hash-Based Algorithm for Accurate Next-Generation Sequencing Short-Read Mapping. *PLoS ONE* 9(3), e90581 .. _Lee2014: http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090581 + + 10.1093/bioinformatics/btu135 + 10.1371/journal.pone.0090581 + diff -r 6adb6e16b00f -r 1fea05cdc1a1 readme.rst --- a/readme.rst Wed Apr 02 12:32:52 2014 -0400 +++ b/readme.rst Thu Nov 06 13:20:15 2014 -0500 @@ -19,6 +19,7 @@ Version history --------------- +- Release 1: Update Orione citation. Upgrade Mosaik dependency to v. 2.2.28 (2.2.30 is buggy, see https://github.com/wanpinglee/MOSAIK/issues/11 ). Use 2.1.78 neural networks. Add package_zlib_1_2_8 and package_samtools_0_1_19 dependencies. Add . - Release 0: Initial release in the Tool Shed. Development diff -r 6adb6e16b00f -r 1fea05cdc1a1 tool_dependencies.xml --- a/tool_dependencies.xml Wed Apr 02 12:32:52 2014 -0400 +++ b/tool_dependencies.xml Thu Nov 06 13:20:15 2014 -0500 @@ -1,11 +1,20 @@ - + + + + git clone https://github.com/wanpinglee/MOSAIK.git mosaik - git reset --hard e04c806bb1410cf1dbd1534991c46d696aec6723 + git reset --hard 24cf066048228a729c79f1de06b403ad8b0bc920 sed -i 's/ -static//g' src/includes/linux.inc + + + + + + make -C src/ bin @@ -16,15 +25,15 @@ $INSTALL_DIR/networkFile - $INSTALL_DIR/bin + $INSTALL_DIR/bin - $INSTALL_DIR/networkFile + $INSTALL_DIR/networkFile - + + +