Mercurial > repos > crs4 > sspace
view sspace.py @ 0:b1be94418db9 draft
Uploaded
author | crs4 |
---|---|
date | Tue, 17 Sep 2013 09:41:18 -0400 |
parents | |
children |
line wrap: on
line source
# -*- coding: utf-8 -*- """ SSPACE wrapper """ import logging import optparse import os import shutil import subprocess import tempfile def which(name, flags=os.X_OK): """ Search PATH for executable files with the given name. """ result = [] exts = filter(None, os.environ.get('PATHEXT', '').split(os.pathsep)) path = os.environ.get('PATH', None) if path is None: return [] for p in os.environ.get('PATH', '').split(os.pathsep): p = os.path.join(p, str(name)) if os.access(p, flags): result.append(p) for e in exts: pext = p + e if os.access(pext, flags): result.append(pext) return result LOG_FORMAT = '%(asctime)s|%(levelname)-8s|%(message)s' LOG_DATEFMT = '%Y-%m-%d %H:%M:%S' LOG_LEVELS = ['DEBUG', 'INFO', 'WARNING', 'ERROR', 'CRITICAL'] def __main__(): """ main function """ parser = optparse.OptionParser() parser.add_option('-c', dest='contigs', help='contigs mandatory (-s)') parser.add_option('--r1', dest='reads1', help='') parser.add_option('--r2', dest='reads2', help='') parser.add_option('-i', dest='insert', type='int', help='') parser.add_option('-e', dest='error', type='float', help='') parser.add_option('-o', dest='orientation', choices=['FF', 'FR', 'RF', 'RR'], help='') parser.add_option('-x', action='store_true', dest='extension', help='whether to extend the contigs (-x)') parser.add_option('--minoverlap', dest='minoverlap', type='int', help='minoverlap (-m)') parser.add_option('--numofreads', dest='numofreads', type='int', help='num of reads to call an extension (-o)') parser.add_option('-t', dest='max_trim', type='int', help='maximum number of bases to trim on the contig end when all possibilities have been exhausted for an extension (-t)') parser.add_option('-u', dest='unpaired', help='unpaired reads (-u, optional)') parser.add_option('-r', dest='min_base_ratio', type='float', help='minimum base ratio used to accept a overhang consensus base (-r)') parser.add_option('--minlink', dest='minlink', type='int', help='min link (-k)') parser.add_option('--maxratio', dest='maxratio', type='float', help='max ratio (-a)') parser.add_option('--contigoverlap', dest='contigoverlap', type='int', help='contigoverlap (-n)') parser.add_option('--mincontig', dest='mincontig', type='int', help='mincontig (-z)') parser.add_option('-T', dest='n_threads', type='int', help='number of threads to use in Bowtie (-T)') parser.add_option('-p', dest='prefix', default='sspace_pre', help='prefix (-b)') parser.add_option('--lib', dest='libraryname', default='galx', help='libraryfile') parser.add_option('--fe', dest='finalevidence', help='') parser.add_option('--fs', dest='finalscaffolds', help='') parser.add_option('--lg', dest='logfile', help='') parser.add_option('--summ', dest='summaryfile', help='') parser.add_option('--loglevel', choices=LOG_LEVELS, help='logging level (default: INFO)', default='DEBUG') (options, args) = parser.parse_args() if len(args) > 0: parser.error('Wrong number of arguments') log_level = getattr(logging, options.loglevel) kwargs = {'format' : LOG_FORMAT, 'datefmt' : LOG_DATEFMT, 'level' : log_level} if options.logfile: kwargs['filename'] = options.logfile logging.basicConfig(**kwargs) logger = logging.getLogger('SSPACE scaffold assembly') logger.debug('Creating temp dir') contigs = options.contigs reads1 = options.reads1 reads2 = options.reads2 insert = options.insert error = options.error orientation = options.orientation extension = '-x 1' if options.extension else '' minoverlap = "-m %d" % options.minoverlap if options.minoverlap is not None else '' numofreads = "-o %d" % options.numofreads if options.numofreads is not None else '' max_trim = "-t %d" % options.max_trim if options.max_trim is not None else '' unpaired = "-u %s" % options.unpaired if options.unpaired else '' min_base_ratio = "-r %s" % options.min_base_ratio if options.min_base_ratio is not None else '' minlink = "-k %d" % options.minlink if options.minlink is not None else '' maxratio = "-a %s" % options.maxratio if options.maxratio is not None else '' contigoverlap = "-n %d" % options.contigoverlap if options.contigoverlap is not None else '' mincontig = "-z %d" % options.mincontig if options.mincontig is not None else '' n_threads = "-T %d" % options.n_threads if options.n_threads is not None else '' prefix = options.prefix libraryname = options.libraryname finalevidence = options.finalevidence finalscaffolds = options.finalscaffolds summaryfile = options.summaryfile exe_name = "SSPACE_Basic_v2.0.pl" # Set library outline = 'lib1 %s %s %d %s %s\n' % (reads1, reads2, insert, error, orientation) with open(libraryname, 'w') as out: out.write(outline) exe = which(exe_name, os.R_OK) command = "perl %s -l %s -s %s %s %s %s %s %s %s %s %s %s %s %s -b %s" % (exe.pop(), libraryname, contigs, extension, minoverlap, numofreads, max_trim, unpaired, min_base_ratio, minlink, maxratio, contigoverlap, mincontig, n_threads, prefix) wd = tempfile.mkdtemp() try: os.chdir(wd) logger.info("SSPACE running") logger.debug("executing %s" % command) subprocess.check_call(args=command, shell=True) # Clean environment with open("%s.logfile.txt" % os.path.join(wd, prefix), 'rb') as sspace_log_file: logger.info("\n".join(["Log from SSPACE", sspace_log_file.read()])) logger.info("Moving result files") shutil.move("%s.final.evidence" % os.path.join(wd, prefix), finalevidence) shutil.move("%s.final.scaffolds.fasta" % os.path.join(wd, prefix), finalscaffolds) shutil.move("%s.summaryfile.txt" % os.path.join(wd, prefix), summaryfile) logger.debug("Removing temp dir: %s" % wd) finally: shutil.rmtree(wd) if __name__ == "__main__": __main__()