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author | cstrittmatter |
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date | Fri, 30 Mar 2018 12:10:40 -0400 |
parents | 4b7f728bcd11 |
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<tool id="ectyper" name="ectyper" version="0.1"> <requirements> <requirement type="package" version="0.1">ectyper</requirement> <requirement type="package" version="3.5">python</requirement> </requirements> <command detect_errors="exit_code"><![CDATA[ #if $jobtype.select == "asm" ln -s $jobtype.draft ${draft.name}.fasta; #else if $jobtype.select == "se" ln -s $jobtype.fastq1 ${fastq1.name}_1.fastq; #else if $jobtype.select == "pe" ln -s $jobtype.fastq1 ${fastq1.name}.fastq; ln -s $jobtype.fastq2 ${fastq2.name}.fastq; #else if $jobtype.select == "cl" mkdir temp_data; #for $collection_file in $jobtype.collection_files cp $collection_file temp_data/.; #end for $__tool_directory__/ectyper -i temp_data -d $percent_identity -l $percent_length -o "./"; cat ./output/output.csv > results.csv; GALAXY_URL #end if #if $jobtype.select != "cl" $__tool_directory__/ectyper #if $jobtype.select == "asm" -i ${draft.name}.fasta #else if $jobtype.select == "se" -i ${fastq1.name}_1.fastq #else if $jobtype.select == "pe" -i ${fastq1.name}.fastq,${fastq2.name}.fastq #end if -d $percent_identity -l $percent_length -o "./"; cat ./output/output.csv > results.csv; #end if ]]></command> <inputs> <conditional name="jobtype"> <param name="select" type="select" label="Assembly or FASTQ Reads?"> <option value="asm">Genome Assembly</option> <option value="se">Single-End Reads</option> <option value="pe">Paired-End Reads</option> <option value="cl">Collection of Reads</option> </param> <when value="asm"> <param name="draft" type="data" format="fasta" label="FASTA" /> </when> <when value="se"> <param name="fastq1" type="data" format="fastq" label="FASTQ" /> </when> <when value="pe"> <param name="fastq1" type="data" format="fastq" label="FASTQ" /> <param name="fastq2" type="data" format="fastq" label="FASTQ" /> </when> <when value="cl"> <param type="data_collection" name="collection_files" format="fastq" collection_type="list" label="FASTQS: Must be a Data Set list built from multiple fastq files" /> </when> </conditional> <param name="percent_identity" type="integer" label="Percent identity required for an allele match [default 90]" value="90" /> <param name="percent_length" type="integer" label="Percent length required for an allele match [default 50]" value="50" /> </inputs> <outputs> <data format="csv" label="ectyper Results" name="${input.name}.ectperResults" from_work_dir="*.csv"/> </outputs> <help><![CDATA[ **Usage: ectyper** **INPUT** A fasta assembly or single or paired end reads test or data set list of fastqs **PERCENTIDENTITY** Percentage of identity wanted to use against the database. From 0 to 100, default is 90%. **PERCENTLENGTH** Percentage of length wanted to use against the database. From 0 to 100, default is 50%. https://github.com/phac-nml/ecoli_serotyping ]]></help> <citations> <citation type="bibtex"> @misc{pope_dashnow_zobel_holt_raven_schultz_inouye_tomita_2014, title={SRST2: ecyper wraps a standalone serotyping module for Escherichia coli. Supports fasta and fastq file formats.}, url={https://www.nml-lnm.gc.ca/}, author={National Microbiology Laboratory }, }</citation> </citations> </tool>