Mercurial > repos > cstrittmatter > mitokmer
diff README.md @ 0:1472b4f4fbfe draft
planemo upload commit a4aeec0f52695b1cbda85ed1faf85d66ba897ab2
author | cstrittmatter |
---|---|
date | Fri, 12 Apr 2019 14:58:18 -0400 |
parents | |
children |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README.md Fri Apr 12 14:58:18 2019 -0400 @@ -0,0 +1,23 @@ +# kmer_id +Mitichondrial read identification by kmer database for Galaxy server + +File kmer_read_m3.cpp +uses gzip library, compile with: +g++ -O3 -std=c++0x kmer_read_m3.cpp -o kmerread -lz + +database files needed in same directory: +(can create with kmer_build, but not described here yet) +mitochondria_data.txt +mitochondria_refkey.txt +mitochondria_count.txt +mitochondria_tree.txt +mitochondria_probes.txt.gz +1a.fasta (test input) + +File kmer_read_m3.py +(Python 2.7) +Run with: +python kmer_read_m3.py -w [working directory] -d [output directory] -i [input filename1] [input filename2] + +Input files can be two paired files (.fastq, .fastq.gz, .fasta, .fasta.gz) or a single file with none as filename2 +Output is .csv file with read count and % abundance for each species.