Mercurial > repos > cstrittmatter > test_eurl_vtec_wgs_pt
comparison scripts/modules/utils.py @ 0:965517909457 draft
planemo upload commit 15239f1674081ab51ab8dd75a9a40cf1bfaa93e8
author | cstrittmatter |
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date | Wed, 22 Jan 2020 08:41:44 -0500 |
parents | |
children | 0cbed1c0a762 |
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-1:000000000000 | 0:965517909457 |
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1 import pickle | |
2 import traceback | |
3 import shlex | |
4 import subprocess | |
5 from threading import Timer | |
6 import shutil | |
7 import time | |
8 import functools | |
9 import os.path | |
10 import sys | |
11 import argparse | |
12 | |
13 | |
14 def start_logger(workdir): | |
15 time_str = time.strftime("%Y%m%d-%H%M%S") | |
16 sys.stdout = Logger(workdir, time_str) | |
17 logfile = sys.stdout.getLogFile() | |
18 return logfile, time_str | |
19 | |
20 | |
21 class Logger(object): | |
22 def __init__(self, out_directory, time_str): | |
23 self.logfile = os.path.join(out_directory, str('run.' + time_str + '.log')) | |
24 self.terminal = sys.stdout | |
25 self.log = open(self.logfile, "w") | |
26 | |
27 def write(self, message): | |
28 self.terminal.write(message) | |
29 self.log.write(message) | |
30 self.log.flush() | |
31 | |
32 def flush(self): | |
33 pass | |
34 | |
35 def getLogFile(self): | |
36 return self.logfile | |
37 | |
38 | |
39 def checkPrograms(programs_version_dictionary): | |
40 print '\n' + 'Checking dependencies...' | |
41 programs = programs_version_dictionary | |
42 which_program = ['which', ''] | |
43 listMissings = [] | |
44 for program in programs: | |
45 which_program[1] = program | |
46 run_successfully, stdout, stderr = runCommandPopenCommunicate(which_program, False, None, False) | |
47 if not run_successfully: | |
48 listMissings.append(program + ' not found in PATH.') | |
49 else: | |
50 print stdout.splitlines()[0] | |
51 if programs[program][0] is None: | |
52 print program + ' (impossible to determine programme version) found at: ' + stdout.splitlines()[0] | |
53 else: | |
54 if program.endswith('.jar'): | |
55 check_version = ['java', '-jar', stdout.splitlines()[0], programs[program][0]] | |
56 programs[program].append(stdout.splitlines()[0]) | |
57 else: | |
58 check_version = [stdout.splitlines()[0], programs[program][0]] | |
59 run_successfully, stdout, stderr = runCommandPopenCommunicate(check_version, False, None, False) | |
60 if stdout == '': | |
61 stdout = stderr | |
62 if program == 'wget': | |
63 version_line = stdout.splitlines()[0].split(' ', 3)[2] | |
64 else: | |
65 version_line = stdout.splitlines()[0].split(' ')[-1] | |
66 replace_characters = ['"', 'v', 'V', '+'] | |
67 for i in replace_characters: | |
68 version_line = version_line.replace(i, '') | |
69 print program + ' (' + version_line + ') found' | |
70 if programs[program][1] == '>=': | |
71 program_found_version = version_line.split('.') | |
72 program_version_required = programs[program][2].split('.') | |
73 if len(program_version_required) == 3: | |
74 if len(program_found_version) == 2: | |
75 program_found_version.append(0) | |
76 else: | |
77 program_found_version[2] = program_found_version[2].split('_')[0] | |
78 for i in range(0, len(program_version_required)): | |
79 if isinstance(program_found_version[i], (int, long)): | |
80 if int(program_found_version[i]) < int(program_version_required[i]): | |
81 listMissings.append('It is required ' + program + ' with version ' + programs[program][1] + ' ' + programs[program][2]) | |
82 else: | |
83 if version_line != programs[program][2]: | |
84 listMissings.append('It is required ' + program + ' with version ' + programs[program][1] + ' ' + programs[program][2]) | |
85 return listMissings | |
86 | |
87 | |
88 def requiredPrograms(): | |
89 programs_version_dictionary = {} | |
90 programs_version_dictionary['rematch.py'] = ['--version', '>=', '3.2'] | |
91 missingPrograms = checkPrograms(programs_version_dictionary) | |
92 if len(missingPrograms) > 0: | |
93 sys.exit('\n' + 'Errors:' + '\n' + '\n'.join(missingPrograms)) | |
94 | |
95 | |
96 def general_information(logfile, version, outdir, time_str): | |
97 # Check if output directory exists | |
98 | |
99 print '\n' + '==========> patho_typing <==========' | |
100 print '\n' + 'Program start: ' + time.ctime() | |
101 | |
102 # Tells where the logfile will be stored | |
103 print '\n' + 'LOGFILE:' | |
104 print logfile | |
105 | |
106 # Print command | |
107 print '\n' + 'COMMAND:' | |
108 script_path = os.path.abspath(sys.argv[0]) | |
109 print sys.executable + ' ' + script_path + ' ' + ' '.join(sys.argv[1:]) | |
110 | |
111 # Print directory where programme was lunch | |
112 print '\n' + 'PRESENT DIRECTORY:' | |
113 present_directory = os.path.abspath(os.getcwd()) | |
114 print present_directory | |
115 | |
116 # Print program version | |
117 print '\n' + 'VERSION:' | |
118 scriptVersionGit(version, present_directory, script_path) | |
119 | |
120 # Check programms | |
121 requiredPrograms() | |
122 | |
123 return script_path | |
124 | |
125 | |
126 def setPATHvariable(doNotUseProvidedSoftware, script_path): | |
127 path_variable = os.environ['PATH'] | |
128 script_folder = os.path.dirname(script_path) | |
129 # Set path to use provided softwares | |
130 if not doNotUseProvidedSoftware: | |
131 bowtie2 = os.path.join(script_folder, 'src', 'bowtie2-2.2.9') | |
132 samtools = os.path.join(script_folder, 'src', 'samtools-1.3.1', 'bin') | |
133 bcftools = os.path.join(script_folder, 'src', 'bcftools-1.3.1', 'bin') | |
134 | |
135 os.environ['PATH'] = str(':'.join([bowtie2, samtools, bcftools, path_variable])) | |
136 | |
137 # Print PATH variable | |
138 print '\n' + 'PATH variable:' | |
139 print os.environ['PATH'] | |
140 | |
141 | |
142 def scriptVersionGit(version, directory, script_path): | |
143 print 'Version ' + version | |
144 | |
145 try: | |
146 os.chdir(os.path.dirname(script_path)) | |
147 command = ['git', 'log', '-1', '--date=local', '--pretty=format:"%h (%H) - Commit by %cn, %cd) : %s"'] | |
148 run_successfully, stdout, stderr = runCommandPopenCommunicate(command, False, 15, False) | |
149 print stdout | |
150 command = ['git', 'remote', 'show', 'origin'] | |
151 run_successfully, stdout, stderr = runCommandPopenCommunicate(command, False, 15, False) | |
152 print stdout | |
153 os.chdir(directory) | |
154 except: | |
155 print 'HARMLESS WARNING: git command possibly not found. The GitHub repository information will not be obtained.' | |
156 | |
157 | |
158 def runTime(start_time): | |
159 end_time = time.time() | |
160 time_taken = end_time - start_time | |
161 hours, rest = divmod(time_taken, 3600) | |
162 minutes, seconds = divmod(rest, 60) | |
163 print 'Runtime :' + str(hours) + 'h:' + str(minutes) + 'm:' + str(round(seconds, 2)) + 's' | |
164 return round(time_taken, 2) | |
165 | |
166 | |
167 def timer(function, name): | |
168 @functools.wraps(function) | |
169 def wrapper(*args, **kwargs): | |
170 print('\n' + 'RUNNING {0}\n'.format(name)) | |
171 start_time = time.time() | |
172 | |
173 results = list(function(*args, **kwargs)) # guarantees return is a list to allow .insert() | |
174 | |
175 time_taken = runTime(start_time) | |
176 print('END {0}'.format(name)) | |
177 | |
178 results.insert(0, time_taken) | |
179 return results | |
180 return wrapper | |
181 | |
182 | |
183 def removeDirectory(directory): | |
184 if os.path.isdir(directory): | |
185 shutil.rmtree(directory) | |
186 | |
187 | |
188 def saveVariableToPickle(variableToStore, pickleFile): | |
189 with open(pickleFile, 'wb') as writer: | |
190 pickle.dump(variableToStore, writer) | |
191 | |
192 | |
193 def extractVariableFromPickle(pickleFile): | |
194 with open(pickleFile, 'rb') as reader: | |
195 variable = pickle.load(reader) | |
196 return variable | |
197 | |
198 | |
199 def trace_unhandled_exceptions(func): | |
200 @functools.wraps(func) | |
201 def wrapped_func(*args, **kwargs): | |
202 try: | |
203 func(*args, **kwargs) | |
204 except: | |
205 print 'Exception in ' + func.__name__ | |
206 traceback.print_exc() | |
207 return wrapped_func | |
208 | |
209 | |
210 def kill_subprocess_Popen(subprocess_Popen, command): | |
211 print 'Command run out of time: ' + str(command) | |
212 subprocess_Popen.kill() | |
213 | |
214 | |
215 def runCommandPopenCommunicate(command, shell_True, timeout_sec_None, print_comand_True): | |
216 run_successfully = False | |
217 if not isinstance(command, basestring): | |
218 command = ' '.join(command) | |
219 command = shlex.split(command) | |
220 | |
221 if print_comand_True: | |
222 print 'Running: ' + ' '.join(command) | |
223 | |
224 if shell_True: | |
225 command = ' '.join(command) | |
226 proc = subprocess.Popen(command, stdout=subprocess.PIPE, stderr=subprocess.PIPE, shell=True) | |
227 else: | |
228 proc = subprocess.Popen(command, stdout=subprocess.PIPE, stderr=subprocess.PIPE) | |
229 | |
230 not_killed_by_timer = True | |
231 if timeout_sec_None is None: | |
232 stdout, stderr = proc.communicate() | |
233 else: | |
234 timer = Timer(timeout_sec_None, kill_subprocess_Popen, args=(proc, command,)) | |
235 timer.start() | |
236 stdout, stderr = proc.communicate() | |
237 timer.cancel() | |
238 not_killed_by_timer = timer.isAlive() | |
239 | |
240 if proc.returncode == 0: | |
241 run_successfully = True | |
242 else: | |
243 if not print_comand_True and not_killed_by_timer: | |
244 print 'Running: ' + str(command) | |
245 if len(stdout) > 0: | |
246 print 'STDOUT' | |
247 print stdout.decode("utf-8") | |
248 if len(stderr) > 0: | |
249 print 'STDERR' | |
250 print stderr.decode("utf-8") | |
251 return run_successfully, stdout, stderr | |
252 | |
253 | |
254 def required_length(tuple_length_options, argument_name): | |
255 class RequiredLength(argparse.Action): | |
256 def __call__(self, parser, args, values, option_string=None): | |
257 if len(values) not in tuple_length_options: | |
258 msg = 'Option {argument_name} requires one of the following number of arguments: {tuple_length_options}'.format( | |
259 argument_name=self.argument_name, tuple_length_options=tuple_length_options) | |
260 raise argparse.ArgumentTypeError(msg) | |
261 setattr(args, self.dest, values) | |
262 return RequiredLength | |
263 | |
264 | |
265 def get_sequence_information(fasta_file, length_extra_seq): | |
266 sequence_dict = {} | |
267 headers = {} | |
268 | |
269 with open(fasta_file, 'rtU') as reader: | |
270 blank_line_found = False | |
271 sequence_counter = 0 | |
272 temp_sequence_dict = {} | |
273 for line in reader: | |
274 line = line.splitlines()[0] | |
275 if len(line) > 0: | |
276 if not blank_line_found: | |
277 if line.startswith('>'): | |
278 if len(temp_sequence_dict) > 0: | |
279 if temp_sequence_dict.values()[0]['length'] - 2 * length_extra_seq > 0: | |
280 sequence_dict[temp_sequence_dict.keys()[0]] = temp_sequence_dict.values()[0] | |
281 headers[temp_sequence_dict.values()[0]['header'].lower()] = sequence_counter | |
282 else: | |
283 print temp_sequence_dict.values()[0]['header'] + ' sequence ignored due to length <= 0' | |
284 temp_sequence_dict = {} | |
285 | |
286 if line[1:].lower() in headers: | |
287 sys.exit('Found duplicated sequence headers') | |
288 | |
289 sequence_counter += 1 | |
290 temp_sequence_dict[sequence_counter] = {'header': line[1:].lower(), 'sequence': '', 'length': 0} | |
291 else: | |
292 temp_sequence_dict[sequence_counter]['sequence'] += line.upper() | |
293 temp_sequence_dict[sequence_counter]['length'] += len(line) | |
294 else: | |
295 sys.exit('It was found a blank line between the fasta file above line ' + line) | |
296 else: | |
297 blank_line_found = True | |
298 | |
299 if len(temp_sequence_dict) > 0: | |
300 if temp_sequence_dict.values()[0]['length'] - 2 * length_extra_seq > 0: | |
301 sequence_dict[temp_sequence_dict.keys()[0]] = temp_sequence_dict.values()[0] | |
302 headers[temp_sequence_dict.values()[0]['header'].lower()] = sequence_counter | |
303 else: | |
304 print temp_sequence_dict.values()[0]['header'] + ' sequence ignored due to length <= 0' | |
305 | |
306 return sequence_dict, headers | |
307 | |
308 | |
309 def simplify_sequence_dict(sequence_dict): | |
310 simple_sequence_dict = {} | |
311 for counter, info in sequence_dict.items(): | |
312 simple_sequence_dict[info['header']] = info | |
313 del simple_sequence_dict[info['header']]['header'] | |
314 return simple_sequence_dict | |
315 | |
316 | |
317 def chunkstring(string, length): | |
318 return (string[0 + i:length + i] for i in range(0, len(string), length)) | |
319 | |
320 | |
321 def clean_headers_sequences(sequence_dict): | |
322 problematic_characters = ["|", " ", ",", ".", "(", ")", "'", "/", ":"] | |
323 # print 'Checking if reference sequences contain ' + str(problematic_characters) + '\n' | |
324 | |
325 headers_changed = False | |
326 new_headers = {} | |
327 for i in sequence_dict: | |
328 if any(x in sequence_dict[i]['header'] for x in problematic_characters): | |
329 for x in problematic_characters: | |
330 sequence_dict[i]['header'] = sequence_dict[i]['header'].replace(x, '_') | |
331 headers_changed = True | |
332 new_headers[sequence_dict[i]['header'].lower()] = i | |
333 | |
334 if headers_changed: | |
335 print 'At least one of the those characters was found. Replacing those with _' + '\n' | |
336 | |
337 return sequence_dict, new_headers |