Mercurial > repos > damion > blast_reporting
view reference_bins.py @ 0:7db7ecc78ad6 draft
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author | damion |
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date | Mon, 02 Mar 2015 20:46:00 -0500 |
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import os.path import common class ReferenceBins: def __init__(self, db_spec_path = None): """ @param db_spec_path string path to fasta databases specification file. This file has format: #value id type active name path 16S_euzby 16S 1 Euzby /usr/local/galaxy/shared/ngs_data/ ... """ self.reference_bins = [] if db_spec_path == None: # Default to the command-line lookup table in code's folder: db_spec_path = os.path.join(os.path.dirname(__file__), 'fasta_reference_dbs.tab') self.fieldSpec = common.FieldSpec(db_spec_path) def __main__(self): pass # Could double check to see if it exists? def build_bins(self, bins, columns): if bins == None: self.reference_bins = [] return False for myfield in bins.strip().strip(';').split(';'): field_spec = myfield.strip().split(':') field_name = field_spec[0].strip() if field_name != '': if not field_name.replace('_','').isalnum(): common.stop_err("Invalid bin name: " + field_name + ':' + myfield) if len(field_spec) < 2: field_spec.append('column') # default grouping = column if len(field_spec) < 3: field_spec.append('') # default filtering = none if len(field_spec) < 4: field_spec.append('') # default no description grouping = field_spec[1].strip() if not grouping in ['table', 'column', 'hidden']: common.stop_err("Invalid bin layout: " + grouping) bin_filter = field_spec[2].strip() if not bin_filter in ['', 'include', 'exclude']: common.stop_err("Invalid bin sort: " + bin_filter) newbin = self.buildBin(field_name, bin_filter) self.reference_bins.append(newbin) field = { # any time we have a bin we want sort descending 'field': field_name, 'group': grouping, 'sort': 'desc', 'label': newbin.name, 'type': 'bin' } columns.append(field) if (field_spec[3] == 'true'): # description field requested field = { 'field': field_name + '_desc', 'group': 'column', 'sort': '', # Allow other sorts???? 'label': newbin.name + ' Description', 'type': 'text' } columns.append(field) def buildBin(self, bin_folder_name, bin_filter): """ Create a lookup table consisting of a dictionary entry for each accession id held in dictionary's file. @param bin_folder_name string name of requested db, e.g 16S_ncbi @param bin_filter string '' or 'include' or 'exclude' """ bin = ReferenceBin(self.fieldSpec, bin_folder_name, bin_filter) try: with open(bin.file_path) as file_in: for line in file_in: # Should always contains succession id #FUTURE: Preprocess so accession ID ready to go. keyValue = line.rstrip().split("\t",1) # keep only first term minus integer portion of id accGeneralId = keyValue[0].split('.')[0] if len(keyValue) >1: description = keyValue[1] else: description = '' bin.lookup[accGeneralId] = description file_in.close() except IOError: stop_err("Reference bin could not be found or opened: " + self.path + bin_folder_name + '/accession_ids.tab') return bin def setStatus(self, record): if len(self.reference_bins) == 0: return #no bins # Use of "extended slices" http://docs.python.org/2.3/whatsnew/section-slices.html # Example sallseqid is 'gi|194753780|ref|XR_046072.1|;gi|195119578|ref|XR_047594.1|;gi|195154052|ref|XR_047967.1|' # Example accs is ['XR_046072.1', 'XR_047594.1', 'XR_047967.1'] # Original code was "[1::2][1::2]" (select every 2nd item, then every 2nd item of that) accs = record.sallseqid.split('|') if common.re_default_ncbi_id.match(record.sseqid): accs = accs[3::4] #Select every 4th item starting offset 4 elif common.re_default_ref_id.match(record.sseqid): accs = accs[1::2] # Check each accession # against each bin. for ptr, bin in enumerate(self.reference_bins): setattr(record, bin.field, '') #Using '','1' not FALSE/TRUE because of tab delim output setattr(record, bin.field + '_desc', '') for acc in accs: accGeneralId = acc.split('.')[0] if accGeneralId in bin.lookup: if bin.exclude: return False setattr(record, bin.field, str(ptr+1)) # Include any bin notes for this item setattr(record, bin.field + '_desc', bin.lookup[accGeneralId]) break # This result has been binned to this bin so break. def __str__(self): return "name: %s dict: %s" % (self.name, str(self.lookup)) class ReferenceBin: def __init__(self, fieldSpec, bin_folder_name, bin_filter): self.lookup = {} self.folder = bin_folder_name self.name = fieldSpec.getAttribute(bin_folder_name, 'name') self.field = bin_folder_name self.path = fieldSpec.getAttribute(bin_folder_name, 'path') self.exclude = bin_filter #absolute path to reference bins folder: /usr/local/galaxy/shared/ngs_data/ self.file_path = os.path.join(self.path + self.folder + '/accession_ids.tab') if __name__ == '__main__': binManager = ReferenceBins() binManager.__main__()