# HG changeset patch # User davide-albanese # Date 1362866488 18000 # Node ID fb797308e55267512e93c291f8d36d23b820bac6 # Parent 27a2d07f4d530da40c65a27ca4ad529616d291d7 Deleted selected files diff -r 27a2d07f4d53 -r fb797308e552 README.rst --- a/README.rst Sat Mar 09 22:58:27 2013 +0100 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,7 +0,0 @@ -QIIME-1.6.0 -=========== - -* Check ID Map - -Author: Davide Albanese - Fondazione Edmud -Mach, 2012 diff -r 27a2d07f4d53 -r fb797308e552 check_id_map.xml --- a/check_id_map.xml Sat Mar 09 22:58:27 2013 +0100 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,120 +0,0 @@ - - - - - Checks user's metadata mapping file for required data, valid - format - - - check_id_map.py - - -check_id_map.py - --m $mapping_fp - -#if str($char_replace): --c $char_replace -#end if - -#if $not_barcoded: --b -#end if - -#if $variable_len_barcodes: --B -#end if - -#if $disable_primer_check: --p -#end if - -#if str($added_demultiplex_field): --j $added_demultiplex_field -#end if -; -rm `basename $mapping_fp .txt`'.html' -; -rm overlib.js -; -mv `basename $mapping_fp .txt`'.log' $out_log -; -mv `basename $mapping_fp .txt`'_corrected.txt' $out_txt - - - - - - - - - - - - - - - - - - - -Check ID Map checks: - -1. The BarcodeSequence, LinkerPrimerSequences, and ReversePrimer fields - have valid IUPAC DNA characters, and BarcodeSequence characters - are non-degenerate (error) - -2. The SampleID, BarcodeSequence, LinkerPrimerSequence, and Description - headers are present (error) - -3. There are not duplicate header fields (error) - -4. There are not duplicate barcodes (error) - -5. Barcodes are of the same length. Suppressed when - variable_len_barcode flag is passed (warning) - -6. The headers do not contain invalid characters (alphanumeric and - underscore only) (warning) - -7. The data fields do not contain invalid characters (alphanumeric, - underscore, space, and +-%./:,; characters) (warning) - -8. SampleID fields are MIENS compliant (only alphanumeric - and . characters). (warning) - -9. There are no duplicates when the primer and variable length - barcodes are appended (error) - -10. There are no duplicates when barcodes and added demultiplex - fields (-j option) are combined (error) - -11. Data fields are not found beyond the Description column (warning) - -Details about the metadata mapping file format can be found here: -http://www.qiime.org/documentation/file_formats.html#metadata-mapping-files - -Errors and warnings are saved to a log file. Errors can be caused -by problems with the headers, invalid characters in barcodes or -primers, or by duplications in SampleIDs or barcodes. - -Warnings can arise from invalid characters and variable length -barcodes that are not specified with the --variable_len_barcode. -Warnings will contain a reference to the cell (row,column) that -the warning arose from. - -In addition to the log file, a 'corrected_mapping' file will be -created. Any invalid characters will be replaced with '.' -characters in the SampleID fields (to enforce MIENS compliance) -and text in other data fields will be replaced with the character -specified by the -c parameter, which is an underscore '_' by -default. - -If pooled primers are used, separate with a comma. For instance, -a pooled set of three 27f primers (used to increase taxonomic -coverage) could be specified in the LinkerPrimerSequence fields as -such: -AGGGTTCGATTCTGGCTCAG,AGAGTTTGATCCTGGCTTAG,AGAATTTGATCTTGGTTCAG - -