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0
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1 args <- commandArgs(trailingOnly = TRUE)
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2
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3 imgt.dir = args[1]
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4 merged.file = args[2]
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5 gene = args[3]
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6
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65
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7 merged = read.table(merged.file, header=T, sep="\t", fill=T, stringsAsFactors=F, comment.char="", quote="")
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8
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9 if(!("Sequence.ID" %in% names(merged))){ #change-o db
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10 print("Change-O DB changing 'SEQUENCE_ID' to 'Sequence.ID'")
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11 names(merged)[which(names[merged] == "SEQUENCE_ID")] = "Sequence.ID"
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12 }
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0
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13
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14 if(gene != "-"){
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15 merged = merged[grepl(paste("^", gene, sep=""), merged$best_match),]
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55
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16 }
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17
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18 if("best_match" %in% names(merged)){
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0
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19 merged = merged[!grepl("unmatched", merged$best_match),]
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20 }
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21
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57
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22 nrow_dat = 0
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23
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0
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24 for(f in list.files(imgt.dir, pattern="*.txt$")){
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25 #print(paste("filtering", f))
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55
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26 path = file.path(imgt.dir, f)
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31
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27 dat = read.table(path, header=T, sep="\t", fill=T, quote="", stringsAsFactors=F, check.names=FALSE, comment.char="")
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0
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28
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29 dat = dat[dat[,"Sequence ID"] %in% merged$Sequence.ID,]
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30
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57
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31 nrow_dat = nrow(dat)
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32
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0
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33 if(nrow(dat) > 0 & grepl("^8_", f)){ #change the FR1 columns to 0 in the "8_..." file
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34 dat[,grepl("^FR1", names(dat))] = 0
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35 }
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36
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37 write.table(dat, path, quote=F, sep="\t", row.names=F, col.names=T, na="")
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38 }
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57
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39
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40 print(paste("Creating new zip for ", gene, "with", nrow_dat, "sequences"))
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