Mercurial > repos > davidvanzessen > shm_csr

annotate baseline/script_xlsx.py @ 91:f387cc1580c6 draft

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"planemo upload commit 6f5bdb4189fcc9028c90365d8edf8d1d7c1cf690"
author rhpvorderman
date Wed, 02 Feb 2022 10:57:36 +0000
parents 729738462297
children
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
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1 import xlrd
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2 import argparse
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3
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4 parser = argparse.ArgumentParser()
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5 parser.add_argument("--input", help="Excel input file containing one or more sheets where column G has the gene annotation, H has the sequence id and J has the sequence")
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6 parser.add_argument("--ref", help="Reference file")
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7 parser.add_argument("--output", help="Output file")
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8
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9 args = parser.parse_args()
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10
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11 gene_column = 6
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12 id_column = 7
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13 seq_column = 8
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14 LETTERS = [x for x in "ABCDEFGHIJKLMNOPQRSTUVWXYZ"]
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15
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16
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17 refdic = dict()
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18 with open(args.ref, 'r') as ref:
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19 currentSeq = ""
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20 currentId = ""
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21 for line in ref.readlines():
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22 if line[0] is ">":
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23 if currentSeq is not "" and currentId is not "":
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24 refdic[currentId[1:]] = currentSeq
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25 currentId = line.rstrip()
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26 currentSeq = ""
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27 else:
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28 currentSeq += line.rstrip()
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29 refdic[currentId[1:]] = currentSeq
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30
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31 currentSeq = ""
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32 currentId = ""
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33 with xlrd.open_workbook(args.input, 'r') as wb:
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34 with open(args.output, 'a') as o:
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35 for sheet in wb.sheets():
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36 if sheet.cell(1,gene_column).value.find("IGHV") < 0:
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37 print("Genes not in column " + LETTERS[gene_column] + ", skipping sheet " + sheet.name)
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38 continue
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39 o.write(">>>" + sheet.name + "\n")
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40 outputdic = dict()
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41 for rowindex in range(1, sheet.nrows):
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42 ref = sheet.cell(rowindex, gene_column).value.replace(">", "")
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43 if ref in outputdic:
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44 outputdic[ref] += [(sheet.cell(rowindex, id_column).value.replace(">", ""), sheet.cell(rowindex, seq_column).value)]
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45 else:
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46 outputdic[ref] = [(sheet.cell(rowindex, id_column).value.replace(">", ""), sheet.cell(rowindex, seq_column).value)]
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47 #print outputdic
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48
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49 for k in list(outputdic.keys()):
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50 if k in refdic:
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51 o.write(">>" + k + "\n")
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52 o.write(refdic[k] + "\n")
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53 for seq in outputdic[k]:
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54 #print seq
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55 o.write(">" + seq[0] + "\n")
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56 o.write(seq[1] + "\n")
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57 else:
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58 print(k + " not in reference, skipping " + k)