comparison baseline/script_xlsx.py @ 0:c33d93683a09 draft

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author davidvanzessen
date Thu, 13 Oct 2016 10:52:24 -0400
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children ba33b94637ca 729738462297
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-1:000000000000 0:c33d93683a09
1 import xlrd
2 import argparse
3
4 parser = argparse.ArgumentParser()
5 parser.add_argument("--input", help="Excel input file containing one or more sheets where column G has the gene annotation, H has the sequence id and J has the sequence")
6 parser.add_argument("--ref", help="Reference file")
7 parser.add_argument("--output", help="Output file")
8
9 args = parser.parse_args()
10
11 gene_column = 6
12 id_column = 7
13 seq_column = 8
14 LETTERS = [x for x in "ABCDEFGHIJKLMNOPQRSTUVWXYZ"]
15
16
17 refdic = dict()
18 with open(args.ref, 'r') as ref:
19 currentSeq = ""
20 currentId = ""
21 for line in ref.readlines():
22 if line[0] is ">":
23 if currentSeq is not "" and currentId is not "":
24 refdic[currentId[1:]] = currentSeq
25 currentId = line.rstrip()
26 currentSeq = ""
27 else:
28 currentSeq += line.rstrip()
29 refdic[currentId[1:]] = currentSeq
30
31 currentSeq = ""
32 currentId = ""
33 with xlrd.open_workbook(args.input, 'r') as wb:
34 with open(args.output, 'a') as o:
35 for sheet in wb.sheets():
36 if sheet.cell(1,gene_column).value.find("IGHV") < 0:
37 print "Genes not in column " + LETTERS[gene_column] + ", skipping sheet " + sheet.name
38 continue
39 o.write(">>>" + sheet.name + "\n")
40 outputdic = dict()
41 for rowindex in range(1, sheet.nrows):
42 ref = sheet.cell(rowindex, gene_column).value.replace(">", "")
43 if ref in outputdic:
44 outputdic[ref] += [(sheet.cell(rowindex, id_column).value.replace(">", ""), sheet.cell(rowindex, seq_column).value)]
45 else:
46 outputdic[ref] = [(sheet.cell(rowindex, id_column).value.replace(">", ""), sheet.cell(rowindex, seq_column).value)]
47 #print outputdic
48
49 for k in outputdic.keys():
50 if k in refdic:
51 o.write(">>" + k + "\n")
52 o.write(refdic[k] + "\n")
53 for seq in outputdic[k]:
54 #print seq
55 o.write(">" + seq[0] + "\n")
56 o.write(seq[1] + "\n")
57 else:
58 print k + " not in reference, skipping " + k