Mercurial > repos > davidvanzessen > shm_csr
view tests/data/intermediate_files/merged.txt @ 90:6809c63d9161 draft
"planemo upload commit fd64827ff6e63df008f6f50ddb8576ad2b1dbb26"
| author | rhpvorderman |
|---|---|
| date | Tue, 25 Jan 2022 11:28:29 +0000 |
| parents | |
| children |
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Sequence.ID Sequence.number Functionality V.GENE.and.allele V.REGION.score V.REGION.identity.. V.REGION.identity.nt V.REGION.identity....with.ins.del.events. V.REGION.identity.nt..with.ins.del.events. J.GENE.and.allele J.REGION.score J.REGION.identity.. J.REGION.identity.nt D.GENE.and.allele D.REGION.reading.frame CDR1.IMGT.length CDR2.IMGT.length CDR3.IMGT.length CDR.IMGT.lengths FR.IMGT.lengths AA.JUNCTION JUNCTION.frame Orientation Functionality.comment V.REGION.potential.ins.del J.GENE.and.allele.comment V.REGION.insertions V.REGION.deletions Sequence X best_match nt_hit_percentage chunk_hit_percentage start_locations V.REGION FR1.IMGT CDR1.IMGT FR2.IMGT CDR2.IMGT FR3.IMGT CDR3.IMGT V.REGION.Nb.of.positions.including.IMGT.gaps..nt. V.REGION.Nb.of.nucleotides V.REGION.Nb.of.identical.nucleotides V.REGION.Nb.of.mutations V.REGION.Nb.of.silent.mutations V.REGION.Nb.of.nonsilent.mutations V.REGION.a.g V.REGION.g.a V.REGION.c.t V.REGION.t.c V.REGION.a.c V.REGION.c.a V.REGION.a.t V.REGION.t.a V.REGION.g.c V.REGION.c.g V.REGION.g.t V.REGION.t.g FR1.IMGT.Nb.of.positions.including.IMGT.gaps..nt. FR1.IMGT.Nb.of.nucleotides FR1.IMGT.Nb.of.identical.nucleotides FR1.IMGT.Nb.of.mutations FR1.IMGT.Nb.of.silent.mutations FR1.IMGT.Nb.of.nonsilent.mutations FR1.IMGT.a.g FR1.IMGT.g.a FR1.IMGT.c.t FR1.IMGT.t.c FR1.IMGT.a.c FR1.IMGT.c.a FR1.IMGT.a.t FR1.IMGT.t.a FR1.IMGT.g.c FR1.IMGT.c.g FR1.IMGT.g.t FR1.IMGT.t.g CDR1.IMGT.Nb.of.positions.including.IMGT.gaps..nt. CDR1.IMGT.Nb.of.nucleotides CDR1.IMGT.Nb.of.identical.nucleotides CDR1.IMGT.Nb.of.mutations CDR1.IMGT.Nb.of.silent.mutations CDR1.IMGT.Nb.of.nonsilent.mutations CDR1.IMGT.a.g CDR1.IMGT.g.a CDR1.IMGT.c.t CDR1.IMGT.t.c CDR1.IMGT.a.c CDR1.IMGT.c.a CDR1.IMGT.a.t CDR1.IMGT.t.a CDR1.IMGT.g.c CDR1.IMGT.c.g CDR1.IMGT.g.t CDR1.IMGT.t.g FR2.IMGT.Nb.of.positions.including.IMGT.gaps..nt. FR2.IMGT.Nb.of.nucleotides FR2.IMGT.Nb.of.identical.nucleotides FR2.IMGT.Nb.of.mutations FR2.IMGT.Nb.of.silent.mutations FR2.IMGT.Nb.of.nonsilent.mutations FR2.IMGT.a.g FR2.IMGT.g.a FR2.IMGT.c.t FR2.IMGT.t.c FR2.IMGT.a.c FR2.IMGT.c.a FR2.IMGT.a.t FR2.IMGT.t.a FR2.IMGT.g.c FR2.IMGT.c.g FR2.IMGT.g.t FR2.IMGT.t.g CDR2.IMGT.Nb.of.positions.including.IMGT.gaps..nt. CDR2.IMGT.Nb.of.nucleotides CDR2.IMGT.Nb.of.identical.nucleotides CDR2.IMGT.Nb.of.mutations CDR2.IMGT.Nb.of.silent.mutations CDR2.IMGT.Nb.of.nonsilent.mutations CDR2.IMGT.a.g CDR2.IMGT.g.a CDR2.IMGT.c.t CDR2.IMGT.t.c CDR2.IMGT.a.c CDR2.IMGT.c.a CDR2.IMGT.a.t CDR2.IMGT.t.a CDR2.IMGT.g.c CDR2.IMGT.c.g CDR2.IMGT.g.t CDR2.IMGT.t.g FR3.IMGT.Nb.of.positions.including.IMGT.gaps..nt. FR3.IMGT.Nb.of.nucleotides FR3.IMGT.Nb.of.identical.nucleotides FR3.IMGT.Nb.of.mutations FR3.IMGT.Nb.of.silent.mutations FR3.IMGT.Nb.of.nonsilent.mutations FR3.IMGT.a.g FR3.IMGT.g.a FR3.IMGT.c.t FR3.IMGT.t.c FR3.IMGT.a.c FR3.IMGT.c.a FR3.IMGT.a.t FR3.IMGT.t.a FR3.IMGT.g.c FR3.IMGT.c.g FR3.IMGT.g.t FR3.IMGT.t.g CDR3.IMGT.Nb.of.positions.including.IMGT.gaps..nt. CDR3.IMGT.Nb.of.nucleotides CDR3.IMGT.Nb.of.identical.nucleotides CDR3.IMGT.Nb.of.mutations CDR3.IMGT.Nb.of.silent.mutations CDR3.IMGT.Nb.of.nonsilent.mutations CDR3.IMGT.a.g CDR3.IMGT.g.a CDR3.IMGT.c.t CDR3.IMGT.t.c CDR3.IMGT.a.c CDR3.IMGT.c.a CDR3.IMGT.a.t CDR3.IMGT.t.a CDR3.IMGT.g.c CDR3.IMGT.c.g CDR3.IMGT.g.t CDR3.IMGT.t.g X.a.t.a t.a.t. X.a.g.g.c.t..a.t. X.a.t..a.g.c.c.t. FR1.IMGT.seq CDR1.IMGT.seq FR2.IMGT.seq CDR2.IMGT.seq FR3.IMGT.seq CDR3.IMGT.seq CDR3.IMGT.AA VGene DGene JGene unique.def VRegionMutations VRegionNucleotides transitionMutations transversionMutations transitionMutationsAtGC totalMutationsAtGC transitionMutationsAtAT totalMutationsAtAT silentMutationsFR silentMutationsCDR nonSilentMutationsFR nonSilentMutationsCDR JY8QFUQ01A0005 14683 productive Homsap IGHV4-39*03 F 929 91.36 201/220 nt NA Homsap IGHJ3*01 F 232 96 48/50 nt Homsap IGHD3-22*01 F 3 10 7 17 10.7.17 [1.17.38.11] CARSITLTVVVMNDAFDVW in-frame + tctccggtggctccatcaacagtagaaattattattggggctggatccgccagcccccagggaagggtttggagtggattggaaatatctattatagtgggaacacctactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgtgcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtctggggccaagggacattggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [304] t78>c|g92>a,S31>N(- - -)|t105>a,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t|c114>t|g147>t|c148>t|g162>a|g164>a,S55>N(- - -)|g191>a,S64>N(- - -)|c204>t|a232>g,I78>V(+ - +)|c245>g,T82>R(- - -)|c249>t|c261>g,F87>L(+ - -)|g275>c,S92>T(+ - +)|g280>c,V94>L(+ - +)|g303>a| t78>c| g92>a,S31>N(- - -)|t105>a,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t|c114>t| g147>t|c148>t|g162>a|g164>a,S55>N(- - -)| g191>a,S64>N(- - -)| c204>t|a232>g,I78>V(+ - +)|c245>g,T82>R(- - -)|c249>t|c261>g,F87>L(+ - -)|g275>c,S92>T(+ - +)|g280>c,V94>L(+ - +)|g303>a| 311 220 201 19 9 10 1 6 5 1 0 0 0 1 2 2 1 0 78 5 4 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 30 25 5 2 3 0 2 2 0 0 0 0 1 0 0 0 0 51 51 47 4 3 1 0 2 1 0 0 0 0 0 0 0 1 0 30 21 20 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 116 114 105 8 3 5 1 1 2 0 0 0 0 0 2 2 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctcc ggtggctccatcaacagtagaaattattat tggggctggatccgccagcccccagggaagggtttggagtggattggaaat atctattatagtgggaacacc tactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgt gcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtc ARSITLTVVVMNDAFDV IGHV4-39 IGHD3-22 IGHJ3 ggtggctccatcaacagtagaaattattat tggggctggatccgccagcccccagggaagggtttggagtggattggaaat atctattatagtgggaacacc tactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgt gcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtc IGG1 18 216 12 6 11 16 1 2 6 2 6 4 JY8QFUQ01A004N 19777 productive (see comment) Homsap IGHV4-39*02 F 920 90.95 201/221 nt 90.05 199/221 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD4-17*01 F 3 10 7 14 10.7.14 [1.17.38.11] CARPGARETNNAWVDW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 63 of V-REGION: 'G' (1 nucleotides at position 99 to 99 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 70 of V-REGION: 'C' (1 nucleotides at position 123 to 123 in the user submitted sequence), (cause frameshift); tctctggtggctccgtcagtaggagtgcctactactggggctggatccgccagcccccagggaaggggctggagtggattgggaccatctattatagtGgggaccacatactccaatccgtcCcctcaagactcgagtcaccatgtccttggacacgtccaagaaccacatctccctgaagctgaattctgtgaccgccgcagacacggctgtttattactgtgcgagacctggggctagggagactaataacgcctgggtcgactggggccagggcaccctggtcaccgtctcctcggcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [297] a88>g,I30>V(+ - +)|c93>t|t102>g,S34>R(- - -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|t108>c,S36>A(- + -)|g164>c,S55>T(+ - +)|t165>c,S55>T(+ - +)|g191>c,S64>T(+ - +)|c195>a|a200>c,Y67>S(+ - -)|c204>t|g221>c,S74>T(+ - +)|a234>g,I78>M(+ + -)|g238>t,V80>L(+ - +)|a240>g,V80>L(+ - +)|t259>a,F87>I(+ - -)|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)|g303>t| a88>g,I30>V(+ - +)|c93>t|t102>g,S34>R(- - -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|t108>c,S36>A(- + -)| g164>c,S55>T(+ - +)|t165>c,S55>T(+ - +)| g191>c,S64>T(+ - +)|c195>a| a200>c,Y67>S(+ - -)|c204>t|g221>c,S74>T(+ - +)|a234>g,I78>M(+ + -)|g238>t,V80>L(+ - +)|a240>g,V80>L(+ - +)|t259>a,F87>I(+ - -)|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)|g303>t| g319>c|a320>c| 318 (320) 227 (229) 207 20 (22) 4 (6) 16 4 1 3 2 1 (2) 1 0 1 4 (5) 0 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 1 5 2 0 1 1 0 0 0 0 1 0 0 1 51 51 49 2 0 2 0 0 0 1 0 0 0 0 1 0 0 0 30 21 19 2 1 1 0 0 0 0 0 1 0 0 1 0 0 0 117 114 104 10 2 8 2 1 2 0 1 0 0 1 1 0 2 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcagtaggagtgcctactac tggggctggatccgccagcccccagggaaggggctggagtggattgggacc atctattatagtgggaccaca tactccaatccgtccctcaagactcgagtcaccatgtccttggacacgtccaagaaccacatctccctgaagctgaattctgtgaccgccgcagacacggctgtttattactgt gcgagacctggggctagggagactaataacgcctgggtcgac ARPGARETNNAWVD IGHV4-39 IGHD4-17 IGHJ4 ggtggctccgtcagtaggagtgcctactac tggggctggatccgccagcccccagggaaggggctggagtggattgggacc atctattatagtgggaccaca tactccaatccgtccctcaagactcgagtcaccatgtccttggacacgtccaagaaccacatctccctgaagctgaattctgtgaccgccgcagacacggctgtttattactgt gcgagacctggggctagggagactaataacgcctgggtcgac IGG1 20 216 10 10 4 11 6 9 2 2 10 6 JY8QFUQ01A006G 17770 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 812 85.52 189/221 nt 85.07 188/221 nt Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD6-13*01 F 3 10 7 16 10.7.16 [1.17.38.11] CARRWARQRLVMDYFDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'G' (1 nucleotides at position 54 to 54 in the user submitted sequence), (cause frameshift); tctctggtgactccatcagtagtactcattactattggggctggatccggcagGcccccagggaggggactggagtgggttgggagtatccactacactgggagcacctactacaactggtccctcaagcatcgagtctctatatcggtggacacatcgagtaaccagttctccctgaggttgaggtctgtgaccgccgctgacacggctgtatactactgtgcgagacgatgggcgaggcaacggctggtcatggactactttgactactggggccagggaatcgtggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcagg NA IGG1 100 100 [302] g83>a,G28>D(- - -)|c93>t|g104>c,S35>T(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|c114>t|c129>g|a143>g,K48>R(+ + +)|g147>a|a157>g,I53>V(+ - +)|t169>c,Y57>H(+ - -)|t171>c,Y57>H(+ - -)|t174>c|g176>c,S59>T(+ - +)|c205>t,P69>W(- - -)|c206>g,P69>W(- - -)|a220>c,S74>H(+ - -)|g221>a,S74>H(+ - -)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|c237>g|a240>g|g246>a|c249>g|a251>g,K84>S(- - -)|g252>t,K84>S(- - -)|a269>g,K90>R(+ + +)|c271>t|c276>g,S92>R(- - -)|a291>t|g303>a|t306>c| g83>a,G28>D(- - -)|c93>t|g104>c,S35>T(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|c114>t| c129>g|a143>g,K48>R(+ + +)|g147>a|a157>g,I53>V(+ - +)| t169>c,Y57>H(+ - -)|t171>c,Y57>H(+ - -)|t174>c|g176>c,S59>T(+ - +)| c205>t,P69>W(- - -)|c206>g,P69>W(- - -)|a220>c,S74>H(+ - -)|g221>a,S74>H(+ - -)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|c237>g|a240>g|g246>a|c249>g|a251>g,K84>S(- - -)|g252>t,K84>S(- - -)|a269>g,K90>R(+ + +)|c271>t|c276>g,S92>R(- - -)|a291>t|g303>a|t306>c| a320>g| 319 (320) 228 (229) 196 32 (33) 13 (14) 19 5 (6) 6 5 4 2 0 2 0 2 5 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 2 4 0 2 2 0 1 0 0 0 1 0 0 0 51 51 47 4 2 2 2 1 0 0 0 0 0 0 0 1 0 0 30 21 17 4 1 3 0 0 0 3 0 0 0 0 1 0 0 0 117 114 96 18 8 10 3 3 3 1 1 0 2 0 0 4 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatcagtagtactcattactat tggggctggatccggcagcccccagggaggggactggagtgggttgggagt atccactacactgggagcacc tactacaactggtccctcaagcatcgagtctctatatcggtggacacatcgagtaaccagttctccctgaggttgaggtctgtgaccgccgctgacacggctgtatactactgt gcgagacgatgggcgaggcaacggctggtcatggactactttgactac ARRWARQRLVMDYFDY IGHV4-39 IGHD6-13 IGHJ4 ggtgactccatcagtagtactcattactat tggggctggatccggcagcccccagggaggggactggagtgggttgggagt atccactacactgggagcacc tactacaactggtccctcaagcatcgagtctctatatcggtggacacatcgagtaaccagttctccctgaggttgaggtctgtgaccgccgctgacacggctgtatactactgt gcgagacgatgggcgaggcaacggctggtcatggactactttgactac IGG1 32 216 20 12 11 19 9 13 10 3 12 7 JY8QFUQ01A018V 3013 productive Homsap IGHV4-61*02 F 811 85.84 188/219 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-9*01 F 2 10 7 15 10.7.15 [1.17.38.11] CARCPNYNIGTRGFDYW in-frame + tctggtgtctccatgagcaatgagtcctattactggacgtggatccggcagcccgtcgggaagggaccggagtggattgggcgcatctacaccagtgggagcaccaattataatccttccctcaagagtcgagtcaccatgtccttagacacgtccaagaggcagttctccctgaagttgacctctatgaccgccgcagacacggccacatatttctgtgcgaggtgtcccaattacaacattgggacccgggggtttgactactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [295] g83>t,G28>V(- - -)|c90>g,I30>M(+ + -)|g101>a,S34>N(- - -)|g104>a,G35>E(- - -)|t105>g,G35>E(- - -)|a106>t|g107>c|t108>c|c111>t|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c137>t,A46>V(+ - +)|t149>c,L50>P(- - -)|t165>c|t171>c|c198>t|c201>t|c204>t|c207>t|a234>g,I78>M(+ + -)|a237>c|g238>t,V80>L(+ - +)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)|c271>t|g275>c,S92>T(+ - +)|g280>a,V94>M(+ - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)|a308>t,Y103>F(- + -)| g83>t,G28>V(- - -)|c90>g,I30>M(+ + -)|g101>a,S34>N(- - -)|g104>a,G35>E(- - -)|t105>g,G35>E(- - -)|a106>t|g107>c|t108>c|c111>t| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c137>t,A46>V(+ - +)|t149>c,L50>P(- - -)|t165>c| t171>c| c198>t|c201>t|c204>t|c207>t|a234>g,I78>M(+ + -)|a237>c|g238>t,V80>L(+ - +)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)|c271>t|g275>c,S92>T(+ - +)|g280>a,V94>M(+ - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)|a308>t,Y103>F(- + -)| a318>g|g319>t|a320>g| 317 (320) 224 (227) 193 31 (34) 12 (15) 19 2 (4) 5 7 5 1 0 2 0 3 3 2 (3) 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 21 9 4 5 0 2 1 1 0 0 1 0 1 1 1 1 51 51 46 5 1 4 0 0 1 2 0 0 0 0 1 1 0 0 30 21 20 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 98 16 6 10 2 3 5 1 1 0 1 0 1 1 1 0 5 (8) 5 5 0 (3) 0 (3) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tct ggtgtctccatgagcaatgagtcctattac tggacgtggatccggcagcccgtcgggaagggaccggagtggattgggcgc atctacaccagtgggagcacc aattataatccttccctcaagagtcgagtcaccatgtccttagacacgtccaagaggcagttctccctgaagttgacctctatgaccgccgcagacacggccacatatttctgt gcgaggtgtcccaattacaacattgggacccgggggtttgactac ARCPNYNIGTRGFDY IGHV4-61 IGHD3-9 IGHJ4 ggtgtctccatgagcaatgagtcctattac tggacgtggatccggcagcccgtcgggaagggaccggagtggattgggcgc atctacaccagtgggagcacc aattataatccttccctcaagagtcgagtcaccatgtccttagacacgtccaagaggcagttctccctgaagttgacctctatgaccgccgcagacacggccacatatttctgt gcgaggtgtcccaattacaacattgggacccgggggtttgactac IGA1 31 216 19 12 12 20 7 11 7 5 14 5 JY8QFUQ01A019O 21075 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F or Homsap IGHV5-51*04 F 905 90.83 198/218 nt 90.37 197/218 nt Homsap IGHJ2*01 F 220 90.57 48/53 nt Homsap IGHD2-21*02 F 2 8 8 19 8.8.19 [1.17.38.11] CARRSYCGGDCYPSYWYFDLW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 65 of V-REGION: 'C' (1 nucleotides at position 103 to 103 in the user submitted sequence), (cause frameshift); ggtctggatacatctttaatatccactggatcgcctgggtccgccagatgcccgggaaaggcctggagtggatggggatcatctatcctggtaactctgagaCccaaatatagcccggccttccaaggccaggtcaccatctcagccgacaggaccaccaataccgcctacctgcagtggcgcggcctgaaggcctcggacaccgccatgtattactgtgcgagacggtcatattgtggtggtgactgctatcctagttactggtacttcgatctctggggccgtggctccttggtcacggtctccgcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 93 94 [308] t75>g|g86>t,S29>I(- - -)|c104>a,T35>N(- + -)|c105>t,T35>N(- + -)|g107>t,S36>I(- - -)|t109>c,Y37>H(+ - -)|g119>c,G40>A(- + -)|g126>c|g184>a,D62>N(+ + -)|t192>g,D64>E(+ - +)|g197>a,R66>K(+ + +)|c201>t|t208>g,S70>A(- + -)|a245>g,K82>R(+ + +)|t247>a,S83>T(+ - +)|t251>c,I84>T(- - -)|g254>a,S85>N(- - -)|c255>t,S85>N(- - -)|a274>c,S92>R(- - -)|a277>g,S93>G(+ + -)| t75>g| g86>t,S29>I(- - -)|c104>a,T35>N(- + -)|c105>t,T35>N(- + -)|g107>t,S36>I(- - -)|t109>c,Y37>H(+ - -)| g119>c,G40>A(- + -)|g126>c| g184>a,D62>N(+ + -)|t192>g,D64>E(+ - +)| g197>a,R66>K(+ + +)|c201>t|t208>g,S70>A(- + -)|a245>g,K82>R(+ + +)|t247>a,S83>T(+ - +)|t251>c,I84>T(- - -)|g254>a,S85>N(- - -)|c255>t,S85>N(- - -)|a274>c,S92>R(- - -)|a277>g,S93>G(+ + -)| a320>g| 319 (320) 225 (226) 205 20 (21) 3 (4) 17 2 (3) 3 3 2 1 1 0 1 2 0 2 3 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 36 24 19 5 0 5 0 0 1 1 0 1 0 0 0 0 2 0 51 51 49 2 1 1 0 0 0 0 0 0 0 0 2 0 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 117 114 104 10 1 9 2 2 2 1 1 0 0 1 0 0 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ggtct ggatacatctttaatatccactgg atcgcctgggtccgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtaactctgagacc aaatatagcccggccttccaaggccaggtcaccatctcagccgacaggaccaccaataccgcctacctgcagtggcgcggcctgaaggcctcggacaccgccatgtattactgt gcgagacggtcatattgtggtggtgactgctatcctagttactggtacttcgatctc ARRSYCGGDCYPSYWYFDL IGHV5-51 IGHD2-21 IGHJ2 ggatacatctttaatatccactgg atcgcctgggtccgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtaactctgagacc aaatatagcccggccttccaaggccaggtcaccatctcagccgacaggaccaccaataccgcctacctgcagtggcgcggcctgaaggcctcggacaccgccatgtattactgt gcgagacggtcatattgtggtggtgactgctatcctagttactggtacttcgatctc IGG1 19 213 10 9 6 11 4 8 2 0 10 7 JY8QFUQ01A01KX 12649 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 810 86.18 187/217 nt NA Homsap IGHJ3*01 F 169 82 41/50 nt Homsap IGHD3-16*01 F 3 8 8 12 8.8.12 [1.17.38.11] CARDDGGHMTLDVW in-frame + gtctggattcacatttagaagctattccatgaattgggtccgccaggctccagggaaggggctggagtgggtctcagctgtcagtggtggtgggggcgccacaaactacgcggagtccgtgaagggccggttcaccatctccagagacaattccagggggacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgtgcgagagatgacggcggacacatgactttagatgtctggggccaagggacaatggtcagcgtctctccggcctccaccaagggcccatcggtcttcccccgtggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtntcgtgggaactcag NA IGG1 93 88 [286, 285] c75>g|c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a175>g,S59>G(+ + -)|t186>g|t189>c|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|t196>a,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|a251>g,K84>R(+ + +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|c287>t,A96>V(+ - +)|g291>a|g297>a|g301>t,V101>L(+ - +)|c309>t|a317>g,K106>R(+ + +)| c75>g| c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a175>g,S59>G(+ + -)|t186>g|t189>c|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| t196>a,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|a251>g,K84>R(+ + +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|c287>t,A96>V(+ - +)|g291>a|g297>a|g301>t,V101>L(+ - +)|c309>t| a317>g,K106>R(+ + +)| 320 225 194 31 10 21 8 4 4 3 0 2 1 1 1 4 2 1 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 21 3 1 2 0 0 0 0 0 2 0 0 0 0 1 0 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 24 17 7 2 5 3 0 0 2 0 0 0 0 1 0 0 1 117 114 97 17 6 11 4 3 3 1 0 0 1 1 0 3 1 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagct gtcagtggtggtgggggcgccaca aactacgcggagtccgtgaagggccggttcaccatctccagagacaattccagggggacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgt gcgagagatgacggcggacacatgactttagatgtc ARDDGGHMTLDV IGHV3-23 IGHD3-16 IGHJ3 ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagct gtcagtggtggtgggggcgccaca aactacgcggagtccgtgaagggccggttcaccatctccagagacaattccagggggacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgt gcgagagatgacggcggacacatgactttagatgtc IGG1 29 213 18 11 8 16 10 13 6 3 13 7 JY8QFUQ01A0207 20991 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 936 92.63 201/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-2*01 F 3 8 8 11 8.8.11 [1.17.38.11] CARFGPLTRPDYW in-frame + ctctggattcacctttaccaactatggcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtggtgaaaccacataccacgcagaatccgtgcagggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgtgcgagattcggaccacttactcgacctgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [282] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)|c164>g,A55>G(- + -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>c,S64>T(+ - +)|t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a214>c,K72>Q(+ - -)|t246>c|c257>a,T86>N(- + -)|g258>t,T86>N(- + -)|c279>t|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)| c164>g,A55>G(- + -)| g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>c,S64>T(+ - +)| t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a214>c,K72>Q(+ - -)|t246>c|c257>a,T86>N(- + -)|g258>t,T86>N(- + -)|c279>t|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| a317>g,K106>R(+ + +)|g319>t|a320>t| 316 (320) 221 (225) 205 (206) 16 (19) 2 (4) 14 (15) 0 (1) 3 1 2 1 2 1 (2) 1 2 2 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 21 3 0 3 0 1 0 0 0 0 0 1 1 0 0 0 117 114 105 9 2 7 0 1 1 2 1 2 1 0 0 0 1 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgaaaccaca taccacgcagaatccgtgcagggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgt gcgagattcggaccacttactcgacctgactac ARFGPLTRPDY IGHV3-23 IGHD2-2 IGHJ4 ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgaaaccaca taccacgcagaatccgtgcagggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgt gcgagattcggaccacttactcgacctgactac IGG2 16 213 6 10 4 11 2 5 2 0 8 6 JY8QFUQ01A02HL 2336 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 959 93.58 204/218 nt NA Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD2-21*01 F 3 8 8 16 8.8.16 [1.17.38.11] CARGRIVSVGMSGWFDPW in-frame - gctctggattcactttcagtaactactggatgtactgggtccgccaagctccagggaaggggctggagtgggtctcacgtattaatggtgatggaagtagtacaagttacgtggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtttattactgtgcgagaggccgaatagtgtcggttggtatgtcaggctggttcgacccctggggccagggaaccctggtctccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] c74>g|c87>t|g107>a,S36>N(- - -)|c118>t,H40>Y(+ - -)|t152>a,V51>E(- + -)|a172>g,S58>G(+ + -)|g186>a|c192>t|c198>t|c203>t,A68>V(+ - +)|g258>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>t|a315>g| c74>g| c87>t|g107>a,S36>N(- - -)| c118>t,H40>Y(+ - -)|t152>a,V51>E(- + -)| a172>g,S58>G(+ + -)|g186>a|c192>t| c198>t|c203>t,A68>V(+ - +)|g258>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>t| a315>g|a320>g| 319 (320) 225 (226) 210 15 (16) 8 (9) 7 2 (3) 2 6 0 0 0 0 1 2 1 1 0 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 22 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 1 0 0 0 0 1 0 0 0 0 30 24 21 3 2 1 1 1 1 0 0 0 0 0 0 0 0 0 117 114 108 6 3 3 0 0 3 0 0 0 0 0 2 0 1 0 7 (8) 7 6 1 (2) 1 (2) 0 1 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gctct ggattcactttcagtaactactgg atgtactgggtccgccaagctccagggaaggggctggagtgggtctcacgt attaatggtgatggaagtagtaca agttacgtggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtttattactgt gcgagaggccgaatagtgtcggttggtatgtcaggctggttcgacccc ARGRIVSVGMSGWFDP IGHV3-74 IGHD2-21 IGHJ5 ggattcactttcagtaactactgg atgtactgggtccgccaagctccagggaaggggctggagtgggtctcacgt attaatggtgatggaagtagtaca agttacgtggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtttattactgt gcgagaggccgaatagtgtcggttggtatgtcaggctggttcgacccc IGA1 13 213 9 4 8 11 1 2 3 3 5 2 JY8QFUQ01A02KS 11205 productive Homsap IGHV3-7*01 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD6-19*01 F 1 8 8 12 8.8.12 [1.17.38.11] CARGGQRTGWYEYW in-frame + ctctggattcacctttagtacctattggatgacttgggtccgccaggctccagggaaggggctggagtgggtggccagcataaaaaatgatggaagtgagaaatcctatgtggactctgtaaagggccgattcaccatctccagagacaacgccgagaactcactgtatttgcaagtgaacaacctgagagccgaggacacggctgtatattactgtgcgagagggggccaaagaactggctggtacgaatattggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtcccagggcttcttccccc NA IGA2 100 100 [284] g107>c,S36>T(+ - +)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|a164>g,N55>S(- - -)|g171>a|c172>a,Q58>N(+ - +)|a174>t,Q58>N(+ - +)|a197>c,Y66>S(+ - -)|g213>a|a250>g,K84>E(+ - -)|c265>t|a271>g,M91>V(+ - -)|g278>a,S93>N(- - -)|g303>a| g107>c,S36>T(+ - +)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|a164>g,N55>S(- - -)| g171>a|c172>a,Q58>N(+ - +)|a174>t,Q58>N(+ - +)| a197>c,Y66>S(+ - -)|g213>a|a250>g,K84>E(+ - -)|c265>t|a271>g,M91>V(+ - -)|g278>a,S93>N(- - -)|g303>a| a320>g| 319 (320) 224 (225) 210 14 (15) 4 (5) 10 3 (4) 4 2 0 1 1 1 0 2 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 48 3 0 3 1 0 1 0 0 0 0 0 1 0 0 0 30 24 21 3 1 2 0 1 0 0 0 1 1 0 0 0 0 0 117 114 107 7 3 4 2 3 1 0 1 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtacctattgg atgacttgggtccgccaggctccagggaaggggctggagtgggtggccagc ataaaaaatgatggaagtgagaaa tcctatgtggactctgtaaagggccgattcaccatctccagagacaacgccgagaactcactgtatttgcaagtgaacaacctgagagccgaggacacggctgtatattactgt gcgagagggggccaaagaactggctggtacgaatat ARGGQRTGWYEY IGHV3-7 IGHD6-19 IGHJ4 ggattcacctttagtacctattgg atgacttgggtccgccaggctccagggaaggggctggagtgggtggccagc ataaaaaatgatggaagtgagaaa tcctatgtggactctgtaaagggccgattcaccatctccagagacaacgccgagaactcactgtatttgcaagtgaacaacctgagagccgaggacacggctgtatattactgt gcgagagggggccaaagaactggctggtacgaatat IGA2 14 213 9 5 6 9 3 5 3 1 7 3 JY8QFUQ01A02XZ 17850 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 990 95.39 207/217 nt NA Homsap IGHJ4*02 F (see comment) 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 8 19 8.8.19 [1.17.38.11] CAKDGQRYCSNGVCSSFFDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcacctacagcagctatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtctcagcaattagtggtggtggtgctagtacataccacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatttgcaaatgaacagcctgagagccgacgacacggccgtatattactgtgcgaaagatggacaacgatattgctctaatggtgtatgctcgtccttctttgactcctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacgggtgtcgtggaactc NA IGG1 100 100 [306] t89>a,F30>Y(- + -)|t90>c,F30>Y(- + -)|t165>a|a175>g,S59>G(+ + -)|g188>c,G63>A(- + -)|c192>t|t199>c,Y67>H(+ - -)|g225>a|c265>t|g291>c,E97>D(+ - +)| t89>a,F30>Y(- + -)|t90>c,F30>Y(- + -)| t165>a| a175>g,S59>G(+ + -)|g188>c,G63>A(- + -)|c192>t| t199>c,Y67>H(+ - -)|g225>a|c265>t|g291>c,E97>D(+ - +)| 320 225 215 10 4 6 1 1 2 2 0 0 0 2 2 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 1 0 0 0 1 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 24 21 3 1 2 1 0 1 0 0 0 0 0 1 0 0 0 117 114 110 4 2 2 0 1 1 1 0 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctacagcagctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagca attagtggtggtggtgctagtaca taccacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatttgcaaatgaacagcctgagagccgacgacacggccgtatattactgt gcgaaagatggacaacgatattgctctaatggtgtatgctcgtccttctttgactcc AKDGQRYCSNGVCSSFFDS IGHV3-23 IGHD2-8 IGHJ4 ggattcacctacagcagctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagca attagtggtggtggtgctagtaca taccacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatttgcaaatgaacagcctgagagccgacgacacggccgtatattactgt gcgaaagatggacaacgatattgctctaatggtgtatgctcgtccttctttgactcc IGG1 10 213 6 4 3 5 3 5 3 1 2 4 JY8QFUQ01A03E3 22041 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 877 89.81 194/216 nt 89.35 193/216 nt Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-9*01 F 2 8 8 10 8.8.10 [1.17.38.11] CVSDLAGQKGSW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 39 of V-REGION: 'G' (1 nucleotides at position 29 to 29 in the user submitted sequence), (cause frameshift); tcaggattcaccttcagtaggtactggaGtgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgtattaatgaagacggcagccacacagatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagttgtatttgcaaatgaacagtctgagaggcgaggacacggctgtctattattgtgtaagcgatttggctggtcagaaaggttcgtggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgtctggtccagggcttcttccccca NA IGA2 93 95 [278] t78>a|c108>g,S36>R(- - -)|c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|c259>t|c265>t|c287>g,A96>G(- + -)|g303>c|c309>t|c314>t,A105>V(+ - +)|a318>c,R106>S(- - -)| t78>a| c108>g,S36>R(- - -)| c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|c259>t|c265>t|c287>g,A96>G(- + -)|g303>c|c309>t| c314>t,A105>V(+ - +)|a318>c,R106>S(- - -)| 320 224 200 24 8 16 2 3 6 4 2 1 0 2 2 2 0 0 78 3 2 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 49 2 0 2 0 0 1 1 0 0 0 0 0 0 0 0 30 24 16 8 3 5 1 2 0 2 1 0 0 1 1 0 0 0 117 114 104 10 4 6 1 1 4 1 0 1 0 0 1 1 0 0 8 8 6 2 0 2 0 0 1 0 1 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tca ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagccacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagttgtatttgcaaatgaacagtctgagaggcgaggacacggctgtctattattgt gtaagcgatttggctggtcagaaaggttcg VSDLAGQKGS IGHV3-74 IGHD3-9 IGHJ4 ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagccacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagttgtatttgcaaatgaacagtctgagaggcgaggacacggctgtctattattgt gtaagcgatttggctggtcagaaaggttcg IGA2 21 213 14 7 8 13 6 8 4 3 8 6 JY8QFUQ01A03N6 11522 productive (see comment) Homsap IGHV4-55*03 P (see comment) 734 82.03 178/217 nt 81.57 177/217 nt Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD3-10*01 F 2 9 7 15 9.7.15 [1.17.38.11] CARDQSVYTAGSYFDSW in-frame + Potentially, because of detected insertion/deletion however the closest V is a pseudogene Low V-REGION identity (82,03% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR3-IMGT at codon 85 of V-REGION: 'G' (1 nucleotides at position 161 to 161 in the user submitted sequence), (cause frameshift); tctctggtgactccatgagtagcgacacgtggtggagctgggtccgccagacgccagagaagggactggaatggattggggagatcaatcaaagagggacgacctcctacaacccgtccctcaggagtcgagtcgtcctgtcagtgggcgagtccaaaaaGtcaattctccctgaggctgacctctgtgaccgccgcggactcggccatctattattgtgcgagagatcagtctgtctatactgcgggaagttactttgactcctggggccagggaacgctggtcaccgtctcttcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [296] c90>g,I30>M(+ + -)|c93>t|t105>c|g107>a,G36>D(- - -)|t108>c,G36>D(- - -)|a110>c,N37>T(- + -)|c111>g,N37>T(- + -)|a117>g,*39>W(- - -)|t119>g,I40>S(- - -)|c133>a,P45>T(+ + -)|c135>g,P45>T(+ + -)|g140>a,G47>E(- - -)|g147>a|g153>a|a165>g|c169>a,H57>N(- - -)|t174>a,H58>Q(- + -)|t177>a,S59>R(- - -)|g191>c,S64>T(+ - +)|c192>g,S64>T(+ - +)|a197>c,Y66>S(+ - -)|a215>g,K72>R(+ + +)|a226>g,I76>V(+ - +)|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|a232>c,M78>L(+ + -)|a240>g|a242>g,D81>G(- - -)|a244>g,T82>E(- - -)|c245>a,T82>E(- - -)|g252>a|c255>t|g258>a|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|a295>t,T99>S(+ - +)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)|c309>t| c90>g,I30>M(+ + -)|c93>t|t105>c|g107>a,G36>D(- - -)|t108>c,G36>D(- - -)|a110>c,N37>T(- + -)|c111>g,N37>T(- + -)| a117>g,*39>W(- - -)|t119>g,I40>S(- - -)|c133>a,P45>T(+ + -)|c135>g,P45>T(+ + -)|g140>a,G47>E(- - -)|g147>a|g153>a|a165>g| c169>a,H57>N(- - -)|t174>a,H58>Q(- + -)|t177>a,S59>R(- - -)|g191>c,S64>T(+ - +)|c192>g,S64>T(+ - +)| a197>c,Y66>S(+ - -)|a215>g,K72>R(+ + +)|a226>g,I76>V(+ - +)|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|a232>c,M78>L(+ + -)|a240>g|a242>g,D81>G(- - -)|a244>g,T82>E(- - -)|c245>a,T82>E(- - -)|g252>a|c255>t|g258>a|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|a295>t,T99>S(+ - +)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)|c309>t| 311 217 178 39 10 29 9 7 4 2 3 3 1 2 3 4 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 27 20 7 2 5 0 1 1 2 1 0 0 0 0 2 0 0 51 51 43 8 3 5 2 3 0 0 0 1 0 0 0 1 0 1 30 21 16 5 0 5 0 0 0 0 0 1 0 2 1 1 0 0 116 114 94 19 5 14 7 3 3 0 2 1 1 0 2 0 0 0 0 aa,40-41(FR1)|ta,108-109(CDR1)|aa,117-118(FR2)|aa,142-143(FR2)|aa,164-165(FR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,108-109(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggta,106-109(CDR1)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatgagtagcgacacgtgg tggagctgggtccgccagacgccagagaagggactggaatggattggggag atcaatcaaagagggacgacc tcctacaacccgtccctcaggagtcgagtcgtcctgtcagtgggcgagtccaaaaatcaattctccctgaggctgacctctgtgaccgccgcggactcggccatctattattgt gcgagagatcagtctgtctatactgcgggaagttactttgactcc ARDQSVYTAGSYFDS IGHV4-55 IGHD3-10 IGHJ4 ggtgactccatgagtagcgacacgtgg tggagctgggtccgccagacgccagagaagggactggaatggattggggag atcaatcaaagagggacgacc tcctacaacccgtccctcaggagtcgagtcgtcctgtcagtgggcgagtccaaaaatcaattctccctgaggctgacctctgtgaccgccgcggactcggccatctattattgt gcgagagatcagtctgtctatactgcgggaagttactttgactcc IGG1 39 213 22 17 11 21 11 18 8 2 19 10 JY8QFUQ01A08XO 16204 productive Homsap IGHV4-61*01 F 1095 100 220/220 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-12*01 F 3 10 7 20 10.7.20 [1.17.38.11] CARVSGYAYGRYGDYLRGVDYW in-frame + ctctggtggctccgtcagcagtggtagttactactggagctggatccggcagcccccagggaagggactggagtggattgggtatatctattacagtgggagcaccaactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgtgcgagggttagtggctacgcgtacggacgctacggtgactatttgcgcggggttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 94 [311, 312] a318>g|a320>t| 317 (320) 225 (228) 225 (226) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtggctccgtcagcagtggtagttactac tggagctggatccggcagcccccagggaagggactggagtggattgggtat atctattacagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgt gcgagggttagtggctacgcgtacggacgctacggtgactatttgcgcggggttgactac ARVSGYAYGRYGDYLRGVDY IGHV4-61 IGHD5-12 IGHJ4 ggtggctccgtcagcagtggtagttactac tggagctggatccggcagcccccagggaagggactggagtggattgggtat atctattacagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgt gcgagggttagtggctacgcgtacggacgctacggtgactatttgcgcggggttgactac IGG1 0 216 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01A0939 10906 productive (see comment) Homsap IGHV6-1*01 F, or Homsap IGHV6-1*02 F 985 93.33 210/225 nt 92.89 209/225 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-8*01 F 2 10 9 19 10.9.19 [1.17.38.11] CARGSSRGGNSDGVYYELDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 54 of V-REGION: 'G' (1 nucleotides at position 80 to 80 in the user submitted sequence), (cause frameshift); tccggggacagtgtctctaccacccgtgctgcttggaactggatcaggcagtccccatcgggaggccttgagtggctggGgaaggacatactacaggtccaagtggcttaatgattatgcagtgtctgtgaaaagtcgaattaccatcaatccagacacatccaagaaccagttttccctgcagttgaaatctgtcattcccgaggacacggctgtttattactgtgcaagaggttccagtcgcggggggaattctgatggggtctactatgaacttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 79 95 [314, 313] g101>c,S34>T(+ - +)|a104>c,N35>T(- + -)|a106>c,S36>R(- - -)|a142>g,R48>G(- - -)|t190>c,Y64>L(- - -)|a191>t,Y64>L(- - -)|a207>g|a228>t|c237>t|c261>t|c271>t|c276>a,N92>K(+ - -)|g282>c|c284>t,T95>I(- - -)|g303>t| g101>c,S34>T(+ - +)|a104>c,N35>T(- + -)|a106>c,S36>R(- - -)| a142>g,R48>G(- - -)| t190>c,Y64>L(- - -)|a191>t,Y64>L(- - -)| a207>g|a228>t|c237>t|c261>t|c271>t|c276>a,N92>K(+ - -)|g282>c|c284>t,T95>I(- - -)|g303>t| a320>g| 319 (320) 232 (233) 217 15 (16) 7 (8) 8 2 (3) 0 4 1 2 1 2 0 2 0 1 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 0 0 0 0 2 0 0 0 1 0 0 0 51 51 50 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 30 27 25 2 0 2 0 0 0 1 0 0 1 0 0 0 0 0 117 114 105 9 7 2 1 0 4 0 0 1 1 0 1 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,5-6(FR1)|aa,40-41(FR1)|aa,103-104(CDR1)|aa,118-119(FR2)|aa,162-163(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,184-185(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|ta,206-207(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,227-228(FR3)|aa,235-236(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,5-6(FR1)|tt,114-115(CDR1)|tt,149-150(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|tt,198-199(FR3)|ta,206-207(FR3)|ta,227-228(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggta,3-6(FR1)|agct,8-11(FR1)|agca,14-17(FR1)|agca,100-103(CDR1)|ggca,128-131(FR2)|ggct,155-158(FR2)|ggta,188-191(CDR2)|agta,204-207(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tgcc,69-72(FR1)|tgct,108-111(CDR1)|aact,118-121(FR2)|tact,169-172(CDR2)|aacc,228-231(FR3)|aacc,235-238(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|aact,274-277(FR3)|tact,307-310(FR3)| tcc ggggacagtgtctctaccacccgtgctgct tggaactggatcaggcagtccccatcgggaggccttgagtggctgggaagg acatactacaggtccaagtggcttaat gattatgcagtgtctgtgaaaagtcgaattaccatcaatccagacacatccaagaaccagttttccctgcagttgaaatctgtcattcccgaggacacggctgtttattactgt gcaagaggttccagtcgcggggggaattctgatggggtctactatgaacttgactac ARGSSRGGNSDGVYYELDY IGHV6-1 IGHD2-8 IGHJ4 ggggacagtgtctctaccacccgtgctgct tggaactggatcaggcagtccccatcgggaggccttgagtggctgggaagg acatactacaggtccaagtggcttaat gattatgcagtgtctgtgaaaagtcgaattaccatcaatccagacacatccaagaaccagttttccctgcagttgaaatctgtcattcccgaggacacggctgtttattactgt gcaagaggttccagtcgcggggggaattctgatggggtctactatgaacttgactac IGA2 15 222 7 8 4 8 3 7 7 0 3 5 JY8QFUQ01A09OY 14018 productive Homsap IGHV3-11*01 F 982 94.52 207/219 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD4-17*01 F 2 8 8 15 8.8.15 [2.17.38.11] CARRRDYGDYGDGFDYW in-frame - gcctctggattcatcttcagtgactactacatgacctggatccgccaggctccagggaaggggctggagtgggtttcatacattcgtagtaatgggagtcccatatacaacgcagactctgggaggggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaatagtctgagagtcgaggacacggccgtgtattactgtgcgagacggcgggactacggtgactacggtgacggctttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [295] c86>t,T29>I(- - -)|g119>c,S40>T(+ - +)|a169>c,S57>R(- - -)|g176>a,S59>N(- - -)|t186>g|a190>c,T64>P(+ + -)|t199>a,Y67>N(- - -)|t212>g,V71>G(- - -)|a215>g,K72>R(+ + +)|c276>t|c279>t|c287>t,A96>V(+ - +)| c86>t,T29>I(- - -)| g119>c,S40>T(+ - +)| a169>c,S57>R(- - -)|g176>a,S59>N(- - -)|t186>g|a190>c,T64>P(+ + -)| t199>a,Y67>N(- - -)|t212>g,V71>G(- - -)|a215>g,K72>R(+ + +)|c276>t|c279>t|c287>t,A96>V(+ - +)| g319>c|a320>g| 318 (320) 225 (227) 213 12 (14) 3 (5) 9 1 (2) 1 4 0 2 0 0 1 1 (2) 0 0 2 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 20 4 1 3 0 1 0 0 2 0 0 0 0 0 0 1 117 114 108 6 2 4 1 0 3 0 0 0 0 1 0 0 0 1 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcatcttcagtgactactac atgacctggatccgccaggctccagggaaggggctggagtgggtttcatac attcgtagtaatgggagtcccata tacaacgcagactctgggaggggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaatagtctgagagtcgaggacacggccgtgtattactgt gcgagacggcgggactacggtgactacggtgacggctttgactac ARRRDYGDYGDGFDY IGHV3-11 IGHD4-17 IGHJ4 ggattcatcttcagtgactactac atgacctggatccgccaggctccagggaaggggctggagtgggtttcatac attcgtagtaatgggagtcccata tacaacgcagactctgggaggggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaatagtctgagagtcgaggacacggccgtgtattactgt gcgagacggcgggactacggtgactacggtgacggctttgactac IGA2 12 213 6 6 5 6 1 6 2 1 5 4 JY8QFUQ01A0C2Y 20069 productive Homsap IGHV3-7*01 F 923 91.74 200/218 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-3*02 F 2 8 8 14 8.8.14 [1.17.38.11] CARGSYWHYDTFGDYW in-frame - cctctggatttacttttaacaactattggatgacctgggttcgccaggctccagggaaggggctggaatgggtggccaacataaaacaacatggaggtgaaacgtactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgtgcgagagggtcgtattggcattatgataccttcggcgactattggggcccgggagtcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [292] c84>t|c87>t|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|c126>t|g153>a|g171>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| c84>t|c87>t|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|c126>t|g153>a| g171>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)| a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| a320>g| 319 (320) 225 (226) 207 18 (19) 7 (8) 11 3 (4) 5 3 1 2 0 0 0 3 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 2 2 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 0 1 0 0 0 30 24 18 6 2 4 2 2 0 0 1 0 0 0 1 0 0 0 117 114 110 4 1 3 1 0 0 0 1 0 0 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggatttacttttaacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccaac ataaaacaacatggaggtgaaacg tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat ARGSYWHYDTFGDY IGHV3-7 IGHD3-3 IGHJ4 ggatttacttttaacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccaac ataaaacaacatggaggtgaaacg tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat IGG1 18 213 12 6 8 12 4 6 3 4 4 7 JY8QFUQ01A0C33 20623 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 958 93.98 203/216 nt 93.52 202/216 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD2-8*01 F 2 8 8 16 8.8.16 [1.17.38.11] CARDRGCTNGACYVADFW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 35 of V-REGION: 'C' (1 nucleotides at position 16 to 16 in the user submitted sequence), (cause frameshift); tctggattcaccttcCgggaactatagcatgaactgggtccgccacgctccagggaaggggctggagtgggtctcctccattagtaatagaggtagtttcaaatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacgggtgtatatttctgtgcgagagatcggggttgtactaatggtgcatgctatgtcgccgacttctggggccagggaacccaggtcaccgtctcctcagccctccaccaagggcccatcggtcttccccctggcaccctcctcccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 86 88 [298, 299] a103>g,S35>G(+ + -)|t105>g,S35>G(+ + -)|g107>a,S36>N(- - -)|g132>c,Q44>H(- + -)|a162>c|g173>a,S58>N(- - -)|t177>a,S59>R(- - -)|a184>g,S62>G(+ + -)|a191>t,Y64>F(- + -)|t194>a,I65>K(- + -)|c299>g,A100>G(- + -)|g303>a|a308>t,Y103>F(- + -)| a103>g,S35>G(+ + -)|t105>g,S35>G(+ + -)|g107>a,S36>N(- - -)| g132>c,Q44>H(- + -)|a162>c| g173>a,S58>N(- - -)|t177>a,S59>R(- - -)|a184>g,S62>G(+ + -)|a191>t,Y64>F(- + -)|t194>a,I65>K(- + -)| c299>g,A100>G(- + -)|g303>a|a308>t,Y103>F(- + -)| 320 224 211 13 2 11 2 3 0 0 1 0 2 2 1 1 0 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 1 0 0 0 0 0 0 0 0 0 1 51 51 49 2 1 1 0 0 0 0 1 0 0 0 1 0 0 0 30 24 19 5 0 5 1 1 0 0 0 0 1 2 0 0 0 0 117 114 111 3 1 2 0 1 0 0 0 0 1 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcgggaactatagc atgaactgggtccgccacgctccagggaaggggctggagtgggtctcctcc attagtaatagaggtagtttcaaa tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacgggtgtatatttctgt gcgagagatcggggttgtactaatggtgcatgctatgtcgccgacttc ARDRGCTNGACYVADF IGHV3-21 IGHD2-8 IGHJ4 ggattcaccttcgggaactatagc atgaactgggtccgccacgctccagggaaggggctggagtgggtctcctcc attagtaatagaggtagtttcaaa tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacgggtgtatatttctgt gcgagagatcggggttgtactaatggtgcatgctatgtcgccgacttc IGG1 13 213 5 8 3 5 2 8 2 0 3 8 JY8QFUQ01A0C4X 19389 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 959 93.58 204/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 2 8 8 15 8.8.15 [1.17.38.11] CARDMGSGRYFEPYDYW in-frame - ggtctggattcatcttcttgaaatatggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaggtatatggtttgatggaagtaatacatactatgcggactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatttgcaactgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagatatgggttcgggaagatattttgaaccttatgactactggggccagggaaccccggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [295] c74>g|c86>t,T29>I(- - -)|a103>t,S35>L(- - -)|g104>t,S35>L(- - -)|t105>g,S35>L(- - -)|g107>a,S36>K(- - -)|c108>a,S36>K(- - -)|t164>g,V55>G(- - -)|a173>t,Y58>F(- + -)|a194>c,K65>T(- - -)|a204>g|c259>g,L87>V(+ - +)|c265>t|a271>c,M91>L(+ + -)| c74>g| c86>t,T29>I(- - -)|a103>t,S35>L(- - -)|g104>t,S35>L(- - -)|t105>g,S35>L(- - -)|g107>a,S36>K(- - -)|c108>a,S36>K(- - -)| t164>g,V55>G(- - -)| a173>t,Y58>F(- + -)|a194>c,K65>T(- - -)| a204>g|c259>g,L87>V(+ - +)|c265>t|a271>c,M91>L(+ + -)| 320 226 212 14 3 11 1 1 2 0 2 1 2 0 0 2 1 2 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 18 6 0 6 0 1 1 0 0 1 1 0 0 0 1 1 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 30 24 22 2 0 2 0 0 0 0 1 0 1 0 0 0 0 0 117 114 110 4 2 2 1 0 1 0 1 0 0 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ggtct ggattcatcttcttgaaatatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaggt atatggtttgatggaagtaataca tactatgcggactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatttgcaactgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagatatgggttcgggaagatattttgaaccttatgactac ARDMGSGRYFEPYDY IGHV3-33 IGHD3-10 IGHJ4 ggattcatcttcttgaaatatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaggt atatggtttgatggaagtaataca tactatgcggactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatttgcaactgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagatatgggttcgggaagatattttgaaccttatgactac IGG1 13 213 4 9 3 6 1 7 2 0 3 8 JY8QFUQ01A0D2K 3842 productive Homsap IGHV3-48*01 F 990 95.39 207/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 3 8 8 13 8.8.13 [1.17.38.11] CARERGNSYGEIDYW in-frame + ctctggtttcaccttcaggagctctggcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagcagtagtactactaccaaatactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagaacgtggaaacagctatggagagattgactactggggccagggaaccctggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG4 100 88 [288] a81>t|t105>g,S35>R(- - -)|a110>c,Y37>S(+ - -)|a112>g,S38>G(+ + -)|t171>c|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t194>a,I65>K(- + -)|a200>g,Y67>C(- - -)|a254>g,N85>S(- - -)| a81>t|t105>g,S35>R(- - -)|a110>c,Y37>S(+ - -)|a112>g,S38>G(+ + -)| t171>c|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t194>a,I65>K(- + -)| a200>g,Y67>C(- - -)|a254>g,N85>S(- - -)| 320 225 215 10 2 8 3 0 0 1 1 0 1 1 2 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 0 0 1 0 1 0 0 0 0 1 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 0 0 0 1 0 0 0 1 2 0 0 0 117 114 112 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggtttcaccttcaggagctctggc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagcagtagtactactaccaaa tactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagaacgtggaaacagctatggagagattgactac ARERGNSYGEIDY IGHV3-48 IGHD5-18 IGHJ4 ggtttcaccttcaggagctctggc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagcagtagtactactaccaaa tactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagaacgtggaaacagctatggagagattgactac IGG4 10 213 4 6 0 2 4 8 0 2 2 6 JY8QFUQ01A0D5E 14282 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 808 86.85 185/213 nt 86.38 184/213 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-10*02 F 3 7 8 12 7.8.12 [0.17.38.11] CVKDISRSGCFEYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 29 of V-REGION: 1 nucleotides (from position 8 in the user submitted sequence), (cause frameshift) tggattccagttagcaactatgccatgagctgggtccgtcaggctcctgggaaggggctggagtgggtctcaactattagtaaagacggtgtttacacctactaccccgactccgcgaagggccgggtcaccatctccagagacaattccaagaatacaatttatttgcaaatgaacagcctgacagccgaggacacggccagatattactgtgtgaaagacatttccaggtcaggctgctttgagtattggggtcagggaaccctgatcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [280] c87>a|t88>g,F30>V(+ - -)|g107>a,S36>N(- - -)|c129>t|a138>t|g163>a,A55>T(- - -)|g172>a,G58>K(- - -)|g173>a,G58>K(- - -)|t174>a,G58>K(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|t177>c,S59>D(- - -)|g188>t,G63>V(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a195>c|g202>c,A68>P(- - -)|a204>c,A68>P(- - -)|t212>c,V71>A(+ - +)|t226>g,F76>V(+ - -)|c255>t|g258>a|c259>a,L87>I(+ + +)|g261>t,L87>I(+ + +)|c265>t|g284>c,R95>T(- - -)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)|c314>t,A105>V(+ - +)| c87>a|t88>g,F30>V(+ - -)|g107>a,S36>N(- - -)| c129>t|a138>t|g163>a,A55>T(- - -)| g172>a,G58>K(- - -)|g173>a,G58>K(- - -)|t174>a,G58>K(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|t177>c,S59>D(- - -)|g188>t,G63>V(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a195>c| g202>c,A68>P(- - -)|a204>c,A68>P(- - -)|t212>c,V71>A(+ - +)|t226>g,F76>V(+ - -)|c255>t|g258>a|c259>a,L87>I(+ + +)|g261>t,L87>I(+ + +)|c265>t|g284>c,R95>T(- - -)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)| c314>t,A105>V(+ - +)| 320 221 192 29 7 22 1 8 4 2 2 2 2 1 2 0 2 3 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 20 3 1 2 0 1 0 0 0 1 0 0 0 0 0 1 51 51 48 3 2 1 0 1 1 0 0 0 1 0 0 0 0 0 30 24 14 10 1 9 1 4 0 1 1 0 1 1 0 0 1 0 117 114 102 12 3 9 0 2 2 1 1 1 0 0 2 0 1 2 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t ggattccagttagcaactatgcc atgagctgggtccgtcaggctcctgggaaggggctggagtgggtctcaact attagtaaagacggtgtttacacc tactaccccgactccgcgaagggccgggtcaccatctccagagacaattccaagaatacaatttatttgcaaatgaacagcctgacagccgaggacacggccagatattactgt gtgaaagacatttccaggtcaggctgctttgagtat VKDISRSGCFEY IGHV3-23 IGHD3-10 IGHJ4 ggattccagttagcaactatgcc atgagctgggtccgtcaggctcctgggaaggggctggagtgggtctcaact attagtaaagacggtgtttacacc tactaccccgactccgcgaagggccgggtcaccatctccagagacaattccaagaatacaatttatttgcaaatgaacagcctgacagccgaggacacggccagatattactgt gtgaaagacatttccaggtcaggctgctttgagtat IGA1 28 212 14 14 11 17 3 11 5 2 10 11 JY8QFUQ01A0DA8 16017 productive Homsap IGHV3-30*01 F, or Homsap IGHV3-30*17 F 873 89.4 194/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD5-24*01 ORF 2 8 8 12 8.8.12 [1.17.38.11] CARDRRPLREFDCW in-frame - ctctggattcaccctctccagctatgctatgcactgggtccgccagtctcccggcaaggggctagagtgggtggcagctatttcatatgatggaagtaaaatatattacgcagactccgtgaggggccgcttctccatctccagagacagttccaagaacactctgcatttgcaaatggacagcctgagacctgaggacacggctacatattactgtgcgagagatagacggccacttagggagtttgactgttggggccagggaaccccggtcgccgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttct NA IGA1 100 100 [284] t88>c,F30>L(+ - -)|a103>t|g104>c|t105>c|g133>t,A45>S(- + -)|a138>c|t164>c,V55>A(+ - +)|a168>t|t192>a,N64>K(+ - -)|a194>t,K65>I(- + -)|c198>t|a215>g,K72>R(+ + +)|a225>c|a229>t,T77>S(+ - +)|a245>g,N82>S(- - -)|g258>t|t262>c,Y88>H(+ - -)|c265>t|a274>g,N92>D(+ + -)|g286>c,A96>P(- - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)| t88>c,F30>L(+ - -)|a103>t|g104>c|t105>c| g133>t,A45>S(- + -)|a138>c|t164>c,V55>A(+ - +)| a168>t|t192>a,N64>K(+ - -)|a194>t,K65>I(- + -)| c198>t|a215>g,K72>R(+ + +)|a225>c|a229>t,T77>S(+ - +)|a245>g,N82>S(- - -)|g258>t|t262>c,Y88>H(+ - -)|c265>t|a274>g,N92>D(+ + -)|g286>c,A96>P(- - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)| 320 225 202 23 9 14 3 2 2 5 2 0 4 1 2 0 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 3 1 0 0 0 2 0 0 1 0 1 0 0 0 51 51 48 3 1 2 0 0 0 1 1 0 0 0 0 0 1 0 30 24 21 3 1 2 0 0 0 0 0 0 2 1 0 0 0 0 117 114 101 13 4 9 3 2 2 2 1 0 1 0 1 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccctctccagctatgct atgcactgggtccgccagtctcccggcaaggggctagagtgggtggcagct atttcatatgatggaagtaaaata tattacgcagactccgtgaggggccgcttctccatctccagagacagttccaagaacactctgcatttgcaaatggacagcctgagacctgaggacacggctacatattactgt gcgagagatagacggccacttagggagtttgactgt ARDRRPLREFDC IGHV3-30 IGHD5-24 IGHJ4 ggattcaccctctccagctatgct atgcactgggtccgccagtctcccggcaaggggctagagtgggtggcagct atttcatatgatggaagtaaaata tattacgcagactccgtgaggggccgcttctccatctccagagacagttccaagaacactctgcatttgcaaatggacagcctgagacctgaggacacggctacatattactgt gcgagagatagacggccacttagggagtttgactgt IGA1 23 213 12 11 4 8 8 15 5 4 11 3 JY8QFUQ01A0DCS 19190 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 878 89.45 195/218 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD4-23*01 ORF 1 8 8 10 8.8.10 [1.17.38.11] CARLGQLALDYW in-frame - gttcgggattcgcttttaccacgtactggatcggctgggtgcgccagatgcccgggaagggcctggagttgatgggaatcatctttcctggtgactctgaggccagatacagcccgtccttccaaggccaggtcaccctctcagccgacacgtccaccaccaccgtctatctgcagtggagcagtctgaggacctcggacaccgccgtgtattactgtgcgagacttgggcaactcgctcttgactactggggcgagggaaccctgatttccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [280] t78>g|a83>t,Y28>F(- + -)|a85>g,S29>A(- + -)|g86>c,S29>A(- + -)|c87>t,S29>A(- + -)|g107>c,S36>T(+ - +)|c108>g,S36>T(+ - +)|a144>g|g155>t,W52>L(+ - -)|g162>a|a170>t,Y57>F(- + -)|t192>g,D64>E(+ - +)|a193>g,T65>A(- - -)|a232>c,I78>L(+ + +)|a245>c,K82>T(- - -)|t251>c,I84>T(- - -)|g254>c,S85>T(+ - +)|c260>t,A87>V(+ - +)|c264>t|c279>t|a284>g,K95>R(+ + +)|g286>a,A96>T(- - -)|a301>g,M101>V(+ - -)| t78>g| a83>t,Y28>F(- + -)|a85>g,S29>A(- + -)|g86>c,S29>A(- + -)|c87>t,S29>A(- + -)|g107>c,S36>T(+ - +)|c108>g,S36>T(+ - +)| a144>g|g155>t,W52>L(+ - -)|g162>a| a170>t,Y57>F(- + -)|t192>g,D64>E(+ - +)|a193>g,T65>A(- - -)| a232>c,I78>L(+ + +)|a245>c,K82>T(- - -)|t251>c,I84>T(- - -)|g254>c,S85>T(+ - +)|c260>t,A87>V(+ - +)|c264>t|c279>t|a284>g,K95>R(+ + +)|g286>a,A96>T(- - -)|a301>g,M101>V(+ - -)| a320>t| 319 (320) 225 (226) 202 23 (24) 5 (6) 18 5 2 4 1 2 0 2 (3) 0 3 1 1 2 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 36 24 18 6 0 6 1 0 1 0 0 0 1 0 2 1 0 0 51 51 48 3 2 1 1 1 0 0 0 0 0 0 0 0 1 0 30 24 21 3 0 3 1 0 0 0 0 0 1 0 0 0 0 1 117 114 104 10 2 8 2 1 3 1 2 0 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| gttcg ggattcgcttttaccacgtactgg atcggctgggtgcgccagatgcccgggaagggcctggagttgatgggaatc atctttcctggtgactctgaggcc agatacagcccgtccttccaaggccaggtcaccctctcagccgacacgtccaccaccaccgtctatctgcagtggagcagtctgaggacctcggacaccgccgtgtattactgt gcgagacttgggcaactcgctcttgactac ARLGQLALDY IGHV5-51 IGHD4-23 IGHJ4 ggattcgcttttaccacgtactgg atcggctgggtgcgccagatgcccgggaagggcctggagttgatgggaatc atctttcctggtgactctgaggcc agatacagcccgtccttccaaggccaggtcaccctctcagccgacacgtccaccaccaccgtctatctgcagtggagcagtctgaggacctcggacaccgccgtgtattactgt gcgagacttgggcaactcgctcttgactac IGG1 22 213 12 10 6 11 6 11 4 0 9 9 JY8QFUQ01A0EF3 13809 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 972 94.47 205/217 nt NA Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD6-13*01 F 2 8 8 16 8.8.16 [1.17.38.11] CARGRIVSVGMSGWFDPW in-frame + ctctggattcaccttcagtcagtactggatgtactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtattaatggtgatggaagtagcacaagctatgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtatattattgtgcaagaggccgtatagtatcagttgggatgtcaggctggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [296] a106>c,S36>Q(- - -)|g107>a,S36>Q(- - -)|c108>g,S36>Q(- - -)|c118>t,H40>Y(+ - -)|a172>g,S58>G(+ + -)|g186>a|c201>t|g258>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>a|c309>t| a106>c,S36>Q(- - -)|g107>a,S36>Q(- - -)|c108>g,S36>Q(- - -)| c118>t,H40>Y(+ - -)| a172>g,S58>G(+ + -)|g186>a| c201>t|g258>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>a|c309>t| a320>g| 319 (320) 224 (225) 212 12 (13) 5 (6) 7 1 (2) 3 4 0 1 0 0 0 2 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 1 0 0 0 0 1 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 22 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 117 114 108 6 4 2 0 1 3 0 0 0 0 0 2 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtcagtactgg atgtactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attaatggtgatggaagtagcaca agctatgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtatattattgt gcaagaggccgtatagtatcagttgggatgtcaggctggttcgacccc ARGRIVSVGMSGWFDP IGHV3-74 IGHD6-13 IGHJ5 ggattcaccttcagtcagtactgg atgtactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attaatggtgatggaagtagcaca agctatgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtatattattgt gcaagaggccgtatagtatcagttgggatgtcaggctggttcgacccc IGA1 12 213 8 4 7 10 1 2 4 1 3 4 JY8QFUQ01A0ESJ 17367 productive (see comment) Homsap IGHV3-30*01 F, or Homsap IGHV3-30*04 F or Homsap IGHV3-30-3*03 F 927 92.17 200/217 nt 91.71 199/217 nt Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F (see comment) 141 77.08 37/48 nt Homsap IGHD5-24*01 ORF 1 8 8 9 8.8.9 [1.17.38.11] CARGMATAESW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 54 of V-REGION: 'G' (1 nucleotides at position 75 to 75 in the user submitted sequence), (cause frameshift); ctctggattcaccttcattagcgaagctatgcactgggtccgccaggctccaggcaaggggcttgagtgggtggGcactaatatcatatgatgagagtgataaacaatatgtagactccgtgaagggccgattcaccatctccagagacaattccaagaacacactatatctgcaaatgaacagcctgagacgtgaggacacggctgtgtattactgtgcgagaggcatggctactgcggagtcctggggccaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [276] g104>t,S35>I(- - -)|t109>g,Y37>E(- - -)|t111>a,Y37>E(- - -)|a150>t|g163>c,V55>L(+ - +)|t165>a,V55>L(+ - +)|g185>a,G62>E(- - -)|a186>g,G62>E(- - -)|a190>g,N64>D(+ + -)|t196>c,Y66>Q(- - -)|c198>a,Y66>Q(- - -)|c201>t|c203>t,A68>V(+ - +)|g258>a|g261>a|g286>c,A96>R(- - -)|c287>g,A96>R(- - -)| g104>t,S35>I(- - -)|t109>g,Y37>E(- - -)|t111>a,Y37>E(- - -)| a150>t|g163>c,V55>L(+ - +)|t165>a,V55>L(+ - +)| g185>a,G62>E(- - -)|a186>g,G62>E(- - -)|a190>g,N64>D(+ + -)| t196>c,Y66>Q(- - -)|c198>a,Y66>Q(- - -)|c201>t|c203>t,A68>V(+ - +)|g258>a|g261>a|g286>c,A96>R(- - -)|c287>g,A96>R(- - -)| a320>g| 319 (320) 224 (225) 207 17 (18) 4 (5) 13 2 (3) 3 2 1 0 1 1 2 2 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 0 0 0 0 1 0 0 1 1 51 51 48 3 1 2 0 0 0 0 0 0 1 1 1 0 0 0 30 24 21 3 0 3 2 1 0 0 0 0 0 0 0 0 0 0 117 114 106 8 3 5 0 2 2 1 0 1 0 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcattagcgaagct atgcactgggtccgccaggctccaggcaaggggcttgagtgggtggcacta atatcatatgatgagagtgataaa caatatgtagactccgtgaagggccgattcaccatctccagagacaattccaagaacacactatatctgcaaatgaacagcctgagacgtgaggacacggctgtgtattactgt gcgagaggcatggctactgcggagtcc ARGMATAES IGHV3-30 IGHD5-24 IGHJ4 ggattcaccttcattagcgaagct atgcactgggtccgccaggctccaggcaaggggcttgagtgggtggcacta atatcatatgatgagagtgataaa caatatgtagactccgtgaagggccgattcaccatctccagagacaattccaagaacacactatatctgcaaatgaacagcctgagacgtgaggacacggctgtgtattactgt gcgagaggcatggctactgcggagtcc IGA1 17 213 8 9 5 10 3 7 4 0 7 6 JY8QFUQ01A0FII 12470 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 846 88.02 191/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-10*01 F 3 8 8 10 8.8.10 [1.17.38.11] CIMDLVGRADYW in-frame + ctctggattcgccttcagttggtattggatgcactgggtccgccaagttccagggaaggggctggagtgggtcgcacgtatgaacgaagatgggagcatcagaaactacgcggactacgtgaagggccggtttaccatctcaagagacaacgccgagaacacactttatctgcaaatgagcagtctgagagccgaggacacggctatatattactgtataatggatctggttgggagggcggactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 100 [278] a85>g,T29>A(- - -)|a106>t,S36>W(- - -)|c108>g,S36>W(- - -)|c111>t|c134>t,A45>V(+ - +)|t152>a,V51>E(- + -)|t160>g,S54>A(- + -)|t168>g,I56>M(+ + -)|t171>c|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t189>c|g191>t,S64>I(- - -)|c194>g,T65>R(- - -)|g197>a,S66>N(- - -)|c209>a,S70>Y(+ - -)|a225>g|c228>t|c237>a|a250>g,K84>E(+ - -)|g258>a|g261>t|a275>g,N92>S(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| a85>g,T29>A(- - -)|a106>t,S36>W(- - -)|c108>g,S36>W(- - -)|c111>t| c134>t,A45>V(+ - +)|t152>a,V51>E(- + -)|t160>g,S54>A(- + -)| t168>g,I56>M(+ + -)|t171>c|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t189>c|g191>t,S64>I(- - -)|c194>g,T65>R(- - -)| g197>a,S66>N(- - -)|c209>a,S70>Y(+ - -)|a225>g|c228>t|c237>a|a250>g,K84>E(+ - -)|g258>a|g261>t|a275>g,N92>S(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g313>a,A105>I(+ - +)|c314>t,A105>I(+ - +)|g317>t,R106>M(- + -)|a318>g,R106>M(- + -)| 312 (320) 217 (225) 191 (195) 26 (30) 8 18 (22) 5 (6) 5 (6) 3 (4) 2 0 2 1 2 0 2 2 (3) 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 1 0 0 0 1 0 0 1 0 0 51 51 48 3 0 3 0 0 1 0 0 0 0 1 0 0 0 1 30 24 16 8 2 6 1 1 0 2 0 0 0 1 0 1 1 1 117 114 103 11 5 6 3 4 1 0 0 2 0 0 0 0 1 0 0 (8) 0 0 (4) 0 (4) 0 0 (4) 0 (1) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcgccttcagttggtattgg atgcactgggtccgccaagttccagggaaggggctggagtgggtcgcacgt atgaacgaagatgggagcatcaga aactacgcggactacgtgaagggccggtttaccatctcaagagacaacgccgagaacacactttatctgcaaatgagcagtctgagagccgaggacacggctatatattactgt ataatggatctggttgggagggcggactat IMDLVGRADY IGHV3-74 IGHD3-10 IGHJ4 ggattcgccttcagttggtattgg atgcactgggtccgccaagttccagggaaggggctggagtgggtcgcacgt atgaacgaagatgggagcatcaga aactacgcggactacgtgaagggccggtttaccatctcaagagacaacgccgagaacacactttatctgcaaatgagcagtctgagagccgaggacacggctatatattactgt ataatggatctggttgggagggcggactat IGA1 26 213 15 11 8 14 7 12 5 3 9 9 JY8QFUQ01A0FO5 15512 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 981 94.93 206/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-8*01 F 2 8 8 8 8.8.8 [1.17.38.11] CARDNYGDYW in-frame + ctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtgttaatggtgatgggggtagcacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcacagtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccgag NA IGA2 100 100 [272] a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 225 214 11 4 7 4 2 0 0 0 0 0 1 3 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 3 0 0 0 0 0 0 0 0 0 0 0 117 114 106 8 4 4 1 2 0 0 0 0 0 1 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac IGA2 11 213 6 5 2 6 4 5 4 0 4 3 JY8QFUQ01A0GVR 19593 productive Homsap IGHV4-61*02 F 884 89.14 197/221 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-24*01 ORF 3 10 7 13 10.7.13 [1.17.38.11] CARDFGGSYNLVAYW in-frame - tctctggtgactccatcagtagtgattctcactactggagttggatccggcagcccgccgggaagggactggagtggattgggcgtgtctacgccagtgggaccaccaattacagcccctccctcaagagtcgagtcaccatttcagtggacacgtccaggaatcaattctccctgaagttgaattctgtgaccgccgctgacacggccgtttatttctgtgcgagagattttggaggttcctacaatttagtggcctactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [292] g83>a,G28>D(- - -)|c93>t|g104>a,G35>D(- - -)|a106>t|g107>c|t109>c,Y37>H(+ - -)|c120>t|a166>g,I56>V(+ - +)|t171>c|a172>g,T58>A(- - -)|g191>c,S64>T(+ - +)|c198>t|a203>g,N68>S(- - -)|a234>t|a240>g|a251>g,K84>R(+ + +)|c255>t|g258>a|c271>t|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)|a291>t|g303>t|a308>t,Y103>F(- + -)| g83>a,G28>D(- - -)|c93>t|g104>a,G35>D(- - -)|a106>t|g107>c|t109>c,Y37>H(+ - -)| c120>t| a166>g,I56>V(+ - +)|t171>c|a172>g,T58>A(- - -)|g191>c,S64>T(+ - +)| c198>t|a203>g,N68>S(- - -)|a234>t|a240>g|a251>g,K84>R(+ + +)|c255>t|g258>a|c271>t|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)|a291>t|g303>t|a308>t,Y103>F(- + -)| 320 229 205 24 13 11 5 4 6 2 0 0 4 0 2 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 3 3 0 2 1 1 0 0 1 0 1 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 21 17 4 1 3 2 0 0 1 0 0 0 0 1 0 0 0 117 114 101 13 8 5 3 2 4 0 0 0 3 0 0 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatcagtagtgattctcactac tggagttggatccggcagcccgccgggaagggactggagtggattgggcgt gtctacgccagtgggaccacc aattacagcccctccctcaagagtcgagtcaccatttcagtggacacgtccaggaatcaattctccctgaagttgaattctgtgaccgccgctgacacggccgtttatttctgt gcgagagattttggaggttcctacaatttagtggcctac ARDFGGSYNLVAY IGHV4-61 IGHD5-24 IGHJ4 ggtgactccatcagtagtgattctcactac tggagttggatccggcagcccgccgggaagggactggagtggattgggcgt gtctacgccagtgggaccacc aattacagcccctccctcaagagtcgagtcaccatttcagtggacacgtccaggaatcaattctccctgaagttgaattctgtgaccgccgctgacacggccgtttatttctgt gcgagagattttggaggttcctacaatttagtggcctac IGG1 24 216 17 7 10 13 7 11 9 4 5 6 JY8QFUQ01A0GVY 18360 productive Homsap IGHV3-9*01 F 810 86.18 187/217 nt NA Homsap IGHJ3*02 F 232 96 48/50 nt Homsap IGHD5-24*01 ORF 3 8 8 17 8.8.17 [1.17.38.11] CVKDRKAGGYNLLDAFDIW in-frame + ctctggattcaccttcgacgactatgtcatgcattgggtccggcaagttccagagaggggcctggagtgggtcgcaggcattaatggggaaagtaatagttttggctctgtggactctgtaaagggccgattcaccatctccagagacaaggccaagaataccctgtatttgcaaatgaatagcctgagagttgaggacacggccttgtattattgtgtgaaagatagaaaagctggtggctacaatctgttggatgcttttgatatctggggccaagggacaatggtcaccgtcgcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [299] t90>c|t105>c|t108>c|c113>t,A38>V(+ - +)|c120>t|c134>t,A45>V(+ - +)|g140>a,G47>E(- - -)|a143>g,K48>R(+ + +)|t160>g,S54>A(- + -)|t165>c|g170>a,S57>N(- - -)|t172>g,W58>G(- - -)|a175>g,N59>E(+ - -)|t177>a,N59>E(+ - -)|g187>a,G63>N(- - -)|g188>a,G63>N(- - -)|c192>t|a193>t,I65>F(+ - -)|a195>t,I65>F(+ - -)|a200>c,Y67>S(+ - -)|c203>t,A68>V(+ - +)|g213>a|c246>g,N82>K(+ - -)|c255>t|t256>a,S86>T(+ - +)|c265>t|c276>t|t279>c|c287>t,A96>V(+ - +)|c309>t|c314>t,A105>V(+ - +)|a315>g,A105>V(+ - +)| t90>c|t105>c|t108>c|c113>t,A38>V(+ - +)| c120>t|c134>t,A45>V(+ - +)|g140>a,G47>E(- - -)|a143>g,K48>R(+ + +)|t160>g,S54>A(- + -)|t165>c| g170>a,S57>N(- - -)|t172>g,W58>G(- - -)|a175>g,N59>E(+ - -)|t177>a,N59>E(+ - -)|g187>a,G63>N(- - -)|g188>a,G63>N(- - -)|c192>t|a193>t,I65>F(+ - -)|a195>t,I65>F(+ - -)| a200>c,Y67>S(+ - -)|c203>t,A68>V(+ - +)|g213>a|c246>g,N82>K(+ - -)|c255>t|t256>a,S86>T(+ - +)|c265>t|c276>t|t279>c|c287>t,A96>V(+ - +)|c309>t| c314>t,A105>V(+ - +)|a315>g,A105>V(+ - +)| 322 227 195 32 12 20 3 5 11 5 1 0 2 2 0 1 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 3 1 0 0 1 3 0 0 0 0 0 0 0 0 51 51 45 6 2 4 1 1 2 1 0 0 0 0 0 0 0 1 30 24 15 9 1 8 1 3 1 0 0 0 2 1 0 0 0 1 117 114 103 11 6 5 0 1 6 1 1 0 0 1 0 1 0 0 10 10 8 2 0 2 1 0 1 0 0 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcgacgactatgtc atgcattgggtccggcaagttccagagaggggcctggagtgggtcgcaggc attaatggggaaagtaatagtttt ggctctgtggactctgtaaagggccgattcaccatctccagagacaaggccaagaataccctgtatttgcaaatgaatagcctgagagttgaggacacggccttgtattattgt gtgaaagatagaaaagctggtggctacaatctgttggatgcttttgatatc VKDRKAGGYNLLDAFDI IGHV3-9 IGHD5-24 IGHJ3 ggattcaccttcgacgactatgtc atgcattgggtccggcaagttccagagaggggcctggagtgggtcgcaggc attaatggggaaagtaatagtttt ggctctgtggactctgtaaagggccgattcaccatctccagagacaaggccaagaataccctgtatttgcaaatgaatagcctgagagttgaggacacggccttgtattattgt gtgaaagatagaaaagctggtggctacaatctgttggatgcttttgatatc IGA1 30 213 22 8 15 16 7 14 8 4 9 9 JY8QFUQ01A0HBK 8950 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 1080 100 217/217 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD3-22*01 F 3 8 8 14 8.8.14 [1.17.38.11] CARDFFIGGYYGMDVW in-frame + ctctggattcaccttcagtagctatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattagtagtagtagtagttacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagatttcttcataggaggctactacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [290] 320 225 225 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtagtagttacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagatttcttcataggaggctactacggtatggacgtc ARDFFIGGYYGMDV IGHV3-21 IGHD3-22 IGHJ6 ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtagtagttacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagatttcttcataggaggctactacggtatggacgtc IGA1 0 213 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01A0IZI 21034 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 938 91.4 202/221 nt 90.5 200/221 nt Homsap IGHJ1*01 F (see comment) 142 76 38/50 nt Homsap IGHD5-18*01 F 3 8 8 6 8.8.6 [2.17.38.11] CASYGQWL in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 61 to 61 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 55 of V-REGION: 'AGT' (3 nucleotides at position 82 to 84 in the user submitted sequence), (do not cause frameshift); cagcctcgggactcatgtttagcagctatgccatgacctgggtccgccaggctccagggaAaggggctggagtgggtctcaAGTgtcagtagtagtactggttatttcacatactacacagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtatattattgcgctagttacgggcagtggctgggccagggaaccctggtcaccgtctcctcagcctccaccaagggccccatcggtcttcccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 94 [277, 276] t78>g|t82>c,F28>L(+ - -)|c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|g119>c,S40>T(+ - +)|c164>t,A55>V(+ - +)|t165>c,A55>V(+ - +)|t167>g,I56>S(- - -)|g172>a,G58>S(+ + -)|g176>c,S59>T(+ - +)|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)|g202>a,A68>T(- - -)|a275>g,N92>S(- - -)|g291>a|c309>t|t312>c| t78>g| t82>c,F28>L(+ - -)|c86>t,T29>M(- - -)|c87>g,T29>M(- - -)| g119>c,S40>T(+ - +)|c164>t,A55>V(+ - +)|t165>c,A55>V(+ - +)| t167>g,I56>S(- - -)|g172>a,G58>S(+ + -)|g176>c,S59>T(+ - +)|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)| g202>a,A68>T(- - -)|a275>g,N92>S(- - -)|g291>a|c309>t|t312>c| g315>t|a317>g,K106>S(- - -)|a318>t,K106>S(- - -)|g319>t| 314 (320) 223 (229) 204 (206) 19 (23) 4 (6) 15 (17) 1 (2) 4 3 3 0 0 1 (2) 0 2 1 2 (4) 2 78 8 7 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 36 24 21 3 0 3 0 0 1 1 0 0 0 0 0 1 0 0 51 51 48 3 0 3 0 0 1 1 0 0 0 0 1 0 0 0 30 24 17 7 0 7 0 2 0 0 0 0 1 0 1 0 2 1 117 114 109 5 3 2 1 2 1 1 0 0 0 0 0 0 0 0 2 (8) 2 2 (4) 0 (4) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (2) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cagcctcg ggactcatgtttagcagctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcagtc agtagtagtactggttatttcaca tactacacagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtatattattgc gctagttacgggcagtgg ASYGQW IGHV3-23 IGHD5-18 IGHJ1 ggactcatgtttagcagctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcagtc agtagtagtactggttatttcaca tactacacagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtatattattgc gctagttacgggcagtgg IGG1 18 213 11 7 7 12 4 6 3 0 5 10 JY8QFUQ01A0LAJ 13009 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 845 86.61 194/224 nt NA Homsap IGHJ1*01 F (see comment) 134 73.08 38/52 nt Homsap IGHD3-22*01 F 2 8 10 11 8.10.11 [1.17.38.11] CTWDSTGYFRLTW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) tctctggactcactttcagtgacgcctggatgacctgggtccgccaggttccagggaaggggctggagtgggttgcccatattagatggaactctgatgactggaccacagactacgctactcccgtgaagggcagattcaccatctcaaaagatatttcagagaacacgctgtatctacaaatgaacagcctgataagcgaggatacaggcgtttattactgtacctgggacagcactggttattttcggcttacctggggccagggaacccaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [288] c74>t|t82>c,F28>L(+ - -)|a106>g,N36>D(+ + -)|g119>c,S40>T(+ - +)|c134>t,A45>V(+ - +)|g161>c,G54>A(- + -)|g164>a,R55>H(- - +)|a170>g,K57>R(+ + +)|a172>t,S58>W(- - -)|c174>g,S58>W(- - -)|a177>c,K59>N(+ - -)|a178>t,T60>S(+ - +)|g185>a,G62>D(- - -)|t186>c,G62>D(- - -)|g187>t,G63>W(- - -)|a192>c|g205>a,A69>T(- - -)|a207>t,A69>T(- - -)|a216>g|g239>a,R80>K(+ + +)|g244>a,D82>I(- - -)|a245>t,D82>I(- - -)|a250>g,K84>E(+ - -)|a252>g,K84>E(+ - -)|g267>a|a284>t,K95>I(- + -)|c287>g,T96>S(+ - +)|c294>t|c299>g,A100>G(- + -)|g303>t| c74>t| t82>c,F28>L(+ - -)|a106>g,N36>D(+ + -)| g119>c,S40>T(+ - +)|c134>t,A45>V(+ - +)|g161>c,G54>A(- + -)|g164>a,R55>H(- - +)| a170>g,K57>R(+ + +)|a172>t,S58>W(- - -)|c174>g,S58>W(- - -)|a177>c,K59>N(+ - -)|a178>t,T60>S(+ - +)|g185>a,G62>D(- - -)|t186>c,G62>D(- - -)|g187>t,G63>W(- - -)|a192>c| g205>a,A69>T(- - -)|a207>t,A69>T(- - -)|a216>g|g239>a,R80>K(+ + +)|g244>a,D82>I(- - -)|a245>t,D82>I(- - -)|a250>g,K84>E(+ - -)|a252>g,K84>E(+ - -)|g267>a|a284>t,K95>I(- + -)|c287>g,T96>S(+ - +)|c294>t|c299>g,A100>G(- + -)|g303>t| a316>t,T106>W(- - -)|c317>g,T106>W(- - -)|a318>g,T106>W(- - -)| 315 (320) 227 (232) 197 (199) 30 (33) 6 24 (27) 5 (6) 6 3 2 2 0 5 (6) 0 2 3 (4) 2 0 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 1 0 0 0 0 0 0 0 0 51 51 47 4 0 4 0 1 1 0 0 0 0 0 2 0 0 0 30 30 21 9 1 8 1 1 0 1 2 0 2 0 0 1 1 0 117 114 100 14 4 10 3 4 1 0 0 0 3 0 0 2 1 0 3 (8) 3 3 (5) 0 (3) 0 0 (3) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 (1) 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| tctct ggactcactttcagtgacgcctgg atgacctgggtccgccaggttccagggaaggggctggagtgggttgcccat attagatggaactctgatgactggaccaca gactacgctactcccgtgaagggcagattcaccatctcaaaagatatttcagagaacacgctgtatctacaaatgaacagcctgataagcgaggatacaggcgtttattactgt acctgggacagcactggttattttcggcttacc TWDSTGYFRLT IGHV3-15 IGHD3-22 IGHJ1 ggactcactttcagtgacgcctgg atgacctgggtccgccaggttccagggaaggggctggagtgggttgcccat attagatggaactctgatgactggaccaca gactacgctactcccgtgaagggcagattcaccatctcaaaagatatttcagagaacacgctgtatctacaaatgaacagcctgataagcgaggatacaggcgtttattactgt acctgggacagcactggttattttcggcttacc IGA1 29 219 15 14 8 15 7 14 4 1 14 10 JY8QFUQ01A0LBC 2331 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 801 85.71 186/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-16*02 F 1 8 8 13 8.8.13 [1.17.38.11] CATYGRFSFSPLDYW in-frame + gtctggattcgacttcagtagttatggcttccattgggtccgccaggctccaggcaaggggctggagtgggtggcttttatgtggactgatggaggagaaatcacctacgcagactccgtgaagggccgattcaccatttccagagacaatgtcaagaagacagtgtatctgcaaatgagcggcctgagagtcgaggacacggctgtctattattgtgcgacttacggaagattctcgttttctccccttgactactggggccagggaacccttgtcactgtctcctcagcatccccggaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgagaacgtggctcatcgcctgcctggtccagggcttnttccccc NA IGA1 93 74 [288] a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|c108>t|a115>t,M39>F(+ - -)|g117>c,M39>F(+ - -)|c120>t|a162>t|g163>t,V55>F(+ - -)|a168>g,I56>M(+ + -)|t172>a,Y58>T(+ - -)|a173>c,Y58>T(+ - -)|a187>g,S63>G(+ + -)|t189>a,S63>G(+ + -)|a190>g,N64>E(+ - -)|t192>a,N64>E(+ - -)|a194>t,K65>I(- + -)|a195>c,K65>I(- + -)|t196>a,Y66>T(+ - -)|a197>c,Y66>T(+ - -)|t201>c|c234>t|t247>g,S83>V(- - -)|c248>t,S83>V(- - -)|c255>g,N85>K(+ - -)|g258>a|c259>g,L87>V(+ - +)|a275>g,N92>S(- - -)|a277>g,S93>G(+ + -)|c287>t,A96>V(+ - +)|g303>c|c309>t| a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|c108>t| a115>t,M39>F(+ - -)|g117>c,M39>F(+ - -)|c120>t|a162>t|g163>t,V55>F(+ - -)| a168>g,I56>M(+ + -)|t172>a,Y58>T(+ - -)|a173>c,Y58>T(+ - -)|a187>g,S63>G(+ + -)|t189>a,S63>G(+ + -)|a190>g,N64>E(+ - -)|t192>a,N64>E(+ - -)|a194>t,K65>I(- + -)|a195>c,K65>I(- + -)| t196>a,Y66>T(+ - -)|a197>c,Y66>T(+ - -)|t201>c|c234>t|t247>g,S83>V(- - -)|c248>t,S83>V(- - -)|c255>g,N85>K(+ - -)|g258>a|c259>g,L87>V(+ - +)|a275>g,N92>S(- - -)|a277>g,S93>G(+ + -)|c287>t,A96>V(+ - +)|g303>c|c309>t| g317>c,R106>T(- - -)|a318>t,R106>T(- - -)|g319>t| 316 (320) 221 (225) 190 (191) 31 (34) 8 (9) 23 (25) 6 1 6 1 3 1 3 (4) 4 2 (3) 2 1 (2) 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 1 0 1 0 0 1 0 0 0 0 0 0 51 51 46 5 2 3 0 0 1 0 0 0 2 0 1 0 1 0 30 24 15 9 0 9 3 0 0 0 2 0 1 3 0 0 0 0 117 114 100 14 5 9 2 1 4 1 1 0 0 1 1 2 0 1 4 (8) 4 4 (5) 0 (3) 0 (1) 0 (2) 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcgacttcagtagttatggc ttccattgggtccgccaggctccaggcaaggggctggagtgggtggctttt atgtggactgatggaggagaaatc acctacgcagactccgtgaagggccgattcaccatttccagagacaatgtcaagaagacagtgtatctgcaaatgagcggcctgagagtcgaggacacggctgtctattattgt gcgacttacggaagattctcgttttctccccttgactac ATYGRFSFSPLDY IGHV3-33 IGHD3-16 IGHJ4 ggattcgacttcagtagttatggc ttccattgggtccgccaggctccaggcaaggggctggagtgggtggctttt atgtggactgatggaggagaaatc acctacgcagactccgtgaagggccgattcaccatttccagagacaatgtcaagaagacagtgtatctgcaaatgagcggcctgagagtcgaggacacggctgtctattattgt gcgacttacggaagattctcgttttctccccttgactac IGA1 31 213 14 17 7 13 7 18 7 1 12 11 JY8QFUQ01A0LEW 6327 productive Homsap IGHV1-46*01 F, or Homsap IGHV1-46*03 F 984 95.79 205/214 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD3-22*01 F 2 8 8 14 8.8.14 [0.17.38.11] CASAPSDSSGYYFSYW in-frame + tggatacaccttcaccagttactatatgcactgggtccgacaggcccctggacaagggcttgagtggatgggaatgatcaaccctagtggcggaagcacaatctacgcacagaacttccagggcagagttgccatgaccagggacacgtccacgagcacagtctacatggagctgagcagcctgagatctgaggacacggccgtgtattactgtgctagcgccccctctgatagtagtggatactacttttcctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA2 71 100 [287] c108>t|g126>c|a165>g,I55>M(+ + -)|t186>c|t189>a|g197>t,S66>I(- - -)|g210>c,K70>N(+ - -)|c228>t|a229>g,T77>A(- - -)|g315>t| c108>t| g126>c|a165>g,I55>M(+ + -)| t186>c|t189>a| g197>t,S66>I(- - -)|g210>c,K70>N(+ - -)|c228>t|a229>g,T77>A(- - -)| g315>t|a318>c,R106>S(- - -)|a320>c| 317 (320) 219 (222) 209 (210) 10 (12) 6 (7) 4 (5) 2 0 2 1 0 (2) 0 0 1 2 0 2 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 1 0 0 0 0 0 0 0 1 0 0 0 30 24 22 2 2 0 0 0 0 1 0 0 0 1 0 0 0 0 117 114 110 4 1 3 1 0 1 0 0 0 0 0 1 0 1 0 5 (8) 5 4 (5) 1 (3) 1 (2) 0 (1) 0 0 0 0 0 (2) 0 0 0 0 0 1 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,162-163(FR2)|ta,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,62-63(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,164-165(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggca,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|aacc,169-172(CDR2)|agct,196-199(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcaccagttactat atgcactgggtccgacaggcccctggacaagggcttgagtggatgggaatg atcaaccctagtggcggaagcaca atctacgcacagaacttccagggcagagttgccatgaccagggacacgtccacgagcacagtctacatggagctgagcagcctgagatctgaggacacggccgtgtattactgt gctagcgccccctctgatagtagtggatactacttttcctac ASAPSDSSGYYFSY IGHV1-46 IGHD3-22 IGHJ4 ggatacaccttcaccagttactat atgcactgggtccgacaggcccctggacaagggcttgagtggatgggaatg atcaaccctagtggcggaagcaca atctacgcacagaacttccagggcagagttgccatgaccagggacacgtccacgagcacagtctacatggagctgagcagcctgagatctgaggacacggccgtgtattactgt gctagcgccccctctgatagtagtggatactacttttcctac IGA2 9 213 5 4 2 5 3 4 2 3 4 0 JY8QFUQ01A0LZ5 9550 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 141 77.08 37/48 nt Homsap IGHD5-18*01 F 3 8 8 10 8.8.10 [1.17.38.11] CANELGYCSGGG in-frame + ctctggattcacctttagtagtcatgtcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaagtattcgtgccagtaatgataggacacactacgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacactgtatttacaaatgtacagcctgagagtcgaggacacggccgtatattactgtgcgaacgaactcggatactgtagtggtgggggggcccagggaaccctggtcaccgtctcctcagcatccccgaccagcccccaagggtcttccccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 84 [281, 278] c105>t|c108>t|t109>c,Y37>H(+ - -)|c113>t,A38>V(+ - +)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|a169>c,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g184>a,G62>N(- - -)|g185>a,G62>N(- - -)|g188>a,G63>D(- - -)|c192>g,S64>R(- - -)|t196>c,Y66>H(+ - -)|g225>c|g258>a|c265>t|g267>a|a274>t,N92>Y(- - -)|c287>t,A96>V(+ - +)|a318>c,K106>N(+ - -)| c105>t|c108>t|t109>c,Y37>H(+ - -)|c113>t,A38>V(+ - +)| g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| a169>c,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g184>a,G62>N(- - -)|g185>a,G62>N(- - -)|g188>a,G63>D(- - -)|c192>g,S64>R(- - -)| t196>c,Y66>H(+ - -)|g225>c|g258>a|c265>t|g267>a|a274>t,N92>Y(- - -)|c287>t,A96>V(+ - +)| a318>c,K106>N(+ - -)| 320 225 204 21 6 15 0 6 5 3 2 0 1 0 2 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 0 3 1 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 0 1 0 0 30 24 17 7 0 7 0 3 0 1 1 0 0 0 1 1 0 0 117 114 107 7 4 3 0 2 2 1 0 0 1 0 1 0 0 0 8 8 7 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtagtcatgtc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaagt attcgtgccagtaatgataggaca cactacgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacactgtatttacaaatgtacagcctgagagtcgaggacacggccgtatattactgt gcgaacgaactcggatactgtagtggtggg ANELGYCSGG IGHV3-23 IGHD5-18 IGHJ4 ggattcacctttagtagtcatgtc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaagt attcgtgccagtaatgataggaca cactacgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacactgtatttacaaatgtacagcctgagagtcgaggacacggccgtatattactgt gcgaacgaactcggatactgtagtggtggg IGA1 20 213 14 6 11 15 3 5 4 2 5 9 JY8QFUQ01A0N2E 9701 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 1080 100 217/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-19*01 F 1 8 8 17 8.8.17 [1.17.38.11] CARVRGGLSSGWYESLDYW in-frame + ctctggattcaccttcagtagctatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattagtagtagtagtagttacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagttcgaggaggtcttagcagtggctggtacgagtctcttgactactggggccagggaaccctggtcaccgtctcctcagggagtgcatccgccccaaccc NA IGM 100 100 [298] a320>t| 319 (320) 224 (225) 224 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtagtagttacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagttcgaggaggtcttagcagtggctggtacgagtctcttgactac ARVRGGLSSGWYESLDY IGHV3-21 IGHD6-19 IGHJ4 ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtagtagttacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagttcgaggaggtcttagcagtggctggtacgagtctcttgactac IGM 0 213 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01A0N8H 20024 productive Homsap IGHV6-1*01 F, or Homsap IGHV6-1*02 F 1013 94.27 214/227 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-22*01 F 2 10 9 14 10.9.14 [1.17.38.11] CARGGNYYDSGGLDYW in-frame - tctccggggacagtgtctctagcagcagtgttgtttggaactggatcaggcagtccccattgagaggccttgagtggctgggaaggacattctacaggtccaggtggtataatgattattcattatctgtgaaaggtcgaataactatcaagccagacgcatccaagaaccagttctccctgcagctgaactctgtgactcccgaggacacggctgtatattactgtgcaagagggggtaattactatgatagtggtggtcttgactattggggccagggaaccctggtcaccgtctcctcagcatcccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [301] a104>g,N35>S(- - -)|c110>t,A37>V(+ - +)|c113>t,A38>V(+ - +)|c140>t,S47>L(- - -)|a170>t,Y57>F(- + -)|a185>g,K62>R(+ + +)|g202>t,A68>S(- + -)|g205>t,V69>L(+ - +)|a220>g,S74>G(+ + -)|c231>t|c237>g,N79>K(+ - -)|a244>g,T82>A(- - -)|g303>a| a104>g,N35>S(- - -)|c110>t,A37>V(+ - +)|c113>t,A38>V(+ - +)| c140>t,S47>L(- - -)| a170>t,Y57>F(- + -)|a185>g,K62>R(+ + +)| g202>t,A68>S(- + -)|g205>t,V69>L(+ - +)|a220>g,S74>G(+ + -)|c231>t|c237>g,N79>K(+ - -)|a244>g,T82>A(- - -)|g303>a| a320>g| 319 (320) 234 (235) 221 13 (14) 2 (3) 11 4 (5) 1 4 0 0 0 1 0 0 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 1 0 2 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 27 25 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 117 114 107 7 2 5 2 1 1 0 0 0 0 0 0 1 2 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,5-6(FR1)|aa,40-41(FR1)|aa,103-104(CDR1)|aa,118-119(FR2)|aa,162-163(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,184-185(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|ta,206-207(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,227-228(FR3)|aa,235-236(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,5-6(FR1)|tt,114-115(CDR1)|tt,149-150(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|tt,198-199(FR3)|ta,206-207(FR3)|ta,227-228(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggta,3-6(FR1)|agct,8-11(FR1)|agca,14-17(FR1)|agca,100-103(CDR1)|ggca,128-131(FR2)|ggct,155-158(FR2)|ggta,188-191(CDR2)|agta,204-207(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tgcc,69-72(FR1)|tgct,108-111(CDR1)|aact,118-121(FR2)|tact,169-172(CDR2)|aacc,228-231(FR3)|aacc,235-238(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|aact,274-277(FR3)|tact,307-310(FR3)| tctcc ggggacagtgtctctagcagcagtgttgtt tggaactggatcaggcagtccccattgagaggccttgagtggctgggaagg acattctacaggtccaggtggtataat gattattcattatctgtgaaaggtcgaataactatcaagccagacgcatccaagaaccagttctccctgcagctgaactctgtgactcccgaggacacggctgtatattactgt gcaagagggggtaattactatgatagtggtggtcttgactat ARGGNYYDSGGLDY IGHV6-1 IGHD3-22 IGHJ4 ggggacagtgtctctagcagcagtgttgtt tggaactggatcaggcagtccccattgagaggccttgagtggctgggaagg acattctacaggtccaggtggtataat gattattcattatctgtgaaaggtcgaataactatcaagccagacgcatccaagaaccagttctccctgcagctgaactctgtgactcccgaggacacggctgtatattactgt gcaagagggggtaattactatgatagtggtggtcttgactat IGA1 13 222 9 4 5 8 4 5 2 0 6 5 JY8QFUQ01A0OC8 13175 productive (see comment) Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30*19 F or Homsap IGHV3-30-5*01 F 972 94.47 205/217 nt 93.09 202/217 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD1-26*01 F 3 8 8 13 8.8.13 [1.17.37.11] CAKSPSQVGYYLDCW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 37 of V-REGION: 'T' (1 nucleotides at position 25 to 25 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 58 to 58 in the user submitted sequence), (cause frameshift); in FR3-IMGT, from codon 77 of V-REGION: 1 nucleotides (from position 137 in the user submitted sequence), (cause frameshift) cctctggattcaccttcagtacctTttggcatgcactgggtccgccaggctcccggcAaaggggctggagtgggtggcaatcatatcaaatgatggaagtaagaaatactacgcagactccgtgaagggccgattcacatttccagagaaaattccgagaacacgctgtatctgcaaatgagcagcctgagagctgaggacacggctgtgtattactgtgcgaaatcaccctcccaagtgggctactacttagactgttggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [289] g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|a138>c|g163>a,V55>I(+ - +)|t165>c,V55>I(+ - +)|t172>a,Y58>N(- - -)|t192>g,N64>K(+ - -)|t201>c|c234>t|c243>a,D81>E(+ - +)|a250>g,K84>E(+ - -)|a275>g,N92>S(- - -)| g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)| a138>c|g163>a,V55>I(+ - +)|t165>c,V55>I(+ - +)| t172>a,Y58>N(- - -)|t192>g,N64>K(+ - -)| t201>c|c234>t|c243>a,D81>E(+ - +)|a250>g,K84>E(+ - -)|a275>g,N92>S(- - -)| g317>a,R106>K(+ + +)|g319>t|a320>c| 316 (320) 221 (225) 209 (210) 12 (15) 3 (5) 9 (10) 2 1 (2) 1 2 1 (2) 1 1 1 1 0 0 (1) 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 1 0 0 0 51 51 48 3 1 2 0 1 0 1 1 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 0 1 0 0 0 1 117 113 108 5 2 3 2 0 1 1 0 1 0 0 0 0 0 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 0 (1) 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtacctttggc atgcactgggtccgccaggctcccggcaaggggctggagtgggtggcaatc atatcaaatgatggaagtaagaaa tactacgcagactccgtgaagggccgattcacatttccagagaaaattccgagaacacgctgtatctgcaaatgagcagcctgagagctgaggacacggctgtgtattactgt gcgaaatcaccctcccaagtgggctactacttagactgt AKSPSQVGYYLDC IGHV3-30 IGHD1-26 IGHJ4 ggattcaccttcagtacctttggc atgcactgggtccgccaggctcccggcaaggggctggagtgggtggcaatc atatcaaatgatggaagtaagaaa tactacgcagactccgtgaagggccgattcacatttccagagaaaattccgagaacacgctgtatctgcaaatgagcagcctgagagctgaggacacggctgtgtattactgt gcgaaatcaccctcccaagtgggctactacttagactgt IGA2 12 212 6 6 2 4 4 8 3 0 5 4 JY8QFUQ01A0OMH 12258 productive (see comment) Homsap IGHV3-74*03 F 846 88.02 191/217 nt 87.56 190/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-13*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVRDSDSSASYFAHW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 13 to 13 in the user submitted sequence), (cause frameshift); ctctggattcacGtttccacacctcctggatgcactgggtccgccaaggtccaggggaggggctaatgtgggtctcacgaatcaatactgatgggagtaacacaatgtacgcggactccgtaaagggccggttcaccatttccagagacaatgccaagaatacggtgtttctgcaaatgaacagtctgaaagccgacgacacggctgtctattattgtgttagagattcagacagtagcgcctcgtacttcgcccactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctcgcctggtccagggcttcttccccca NA IGA1 79 89 [288] c87>t|a103>c,S35>H(+ - -)|g104>a,S35>H(+ - -)|t105>c,S35>H(+ - -)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|c134>g,A45>G(- + -)|a142>g,K48>E(+ - -)|g150>a|g151>a,V51>M(+ - -)|t165>a|t168>c|g173>c,S58>T(+ - +)|g191>a,S64>N(- - -)|c197>t,T66>M(- - -)|g213>a|a225>g|c234>t|c246>t|c255>t|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|g284>a,R95>K(+ + +)|g291>c,E97>D(+ - +)|g303>c|c309>t|c314>t,A105>V(+ - +)|a315>t,A105>V(+ - +)| c87>t|a103>c,S35>H(+ - -)|g104>a,S35>H(+ - -)|t105>c,S35>H(+ - -)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)| c134>g,A45>G(- + -)|a142>g,K48>E(+ - -)|g150>a|g151>a,V51>M(+ - -)|t165>a| t168>c|g173>c,S58>T(+ - +)|g191>a,S64>N(- - -)| c197>t,T66>M(- - -)|g213>a|a225>g|c234>t|c246>t|c255>t|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|g284>a,R95>K(+ + +)|g291>c,E97>D(+ - +)|g303>c|c309>t| c314>t,A105>V(+ - +)|a315>t,A105>V(+ - +)| 320 225 197 28 11 17 2 6 7 2 2 0 2 1 4 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 1 5 0 1 1 1 2 0 0 0 1 0 0 0 51 51 46 5 2 3 1 2 0 0 0 0 0 1 0 1 0 0 30 24 21 3 1 2 0 1 0 1 0 0 0 0 1 0 0 0 117 114 102 12 7 5 1 2 5 0 0 0 1 0 2 1 0 0 8 8 6 2 0 2 0 0 1 0 0 0 1 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcactttccacacctcctgg atgcactgggtccgccaaggtccaggggaggggctaatgtgggtctcacga atcaatactgatgggagtaacaca atgtacgcggactccgtaaagggccggttcaccatttccagagacaatgccaagaatacggtgtttctgcaaatgaacagtctgaaagccgacgacacggctgtctattattgt gttagagattcagacagtagcgcctcgtacttcgcccac VRDSDSSASYFAH IGHV3-74 IGHD6-13 IGHJ4 ggattcactttccacacctcctgg atgcactgggtccgccaaggtccaggggaggggctaatgtgggtctcacga atcaatactgatgggagtaacaca atgtacgcggactccgtaaagggccggttcaccatttccagagacaatgccaagaatacggtgtttctgcaaatgaacagtctgaaagccgacgacacggctgtctattattgt gttagagattcagacagtagcgcctcgtacttcgcccac IGA1 26 213 16 10 12 18 4 8 9 2 8 7 JY8QFUQ01A0OTP 12495 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 930 92.99 199/214 nt 92.52 198/214 nt Homsap IGHJ5*02 F 183 84.31 43/51 nt Homsap IGHD5-24*01 ORF 1 8 7 12 8.7.12 [1.16.38.11] CARAPRTATLLDPW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 47 of V-REGION: 1 nucleotides (from position 56 in the user submitted sequence), (cause frameshift) tctctggcgactccatcagtggtcactactggagctggatcaggcagcccccaggaagggactgcagtggattggttacatctatcacagtgggagcaccaactacaacccctccctcgagagtcgagtctccatttcagtagacacgtccaagaaccagttctccctgaggttgagttctgtgaccgctgcggacacggccgtgtattactgtgcgagagccccgaggacggctacacttctcgacccctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [282] t81>c|g83>a,G28>D(- - -)|a106>g,S36>G(+ + -)|t109>c,Y37>H(+ - -)|c127>a|g151>c,E51>Q(+ + -)|g162>t|t165>c|t172>c,Y58>H(+ - -)|a214>g,K72>E(+ - -)|a229>t,T77>S(+ - +)|a234>t|a269>g,K90>R(+ + +)|c271>t|c276>t| t81>c|g83>a,G28>D(- - -)|a106>g,S36>G(+ + -)|t109>c,Y37>H(+ - -)| c127>a|g151>c,E51>Q(+ + -)|g162>t|t165>c| t172>c,Y58>H(+ - -)| a214>g,K72>E(+ - -)|a229>t,T77>S(+ - +)|a234>t|a269>g,K90>R(+ + +)|c271>t|c276>t| a320>c| 319 (320) 221 (222) 206 15 (16) 7 (8) 8 3 1 2 4 0 (1) 1 2 0 1 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 0 2 0 0 0 0 0 0 0 0 51 50 46 4 3 1 0 0 0 1 0 1 0 0 1 0 1 0 30 21 20 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 117 114 108 6 3 3 2 0 2 0 0 0 2 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggcgactccatcagtggtcactac tggagctggatcaggcagcccccaggaagggactgcagtggattggttac atctatcacagtgggagcacc aactacaacccctccctcgagagtcgagtctccatttcagtagacacgtccaagaaccagttctccctgaggttgagttctgtgaccgctgcggacacggccgtgtattactgt gcgagagccccgaggacggctacacttctcgacccc ARAPRTATLLDP IGHV4-59 IGHD5-24 IGHJ5 ggcgactccatcagtggtcactac tggagctggatcaggcagcccccaggaagggactgcagtggattggttac atctatcacagtgggagcacc aactacaacccctccctcgagagtcgagtctccatttcagtagacacgtccaagaaccagttctccctgaggttgagttctgtgaccgctgcggacacggccgtgtattactgt gcgagagccccgaggacggctacacttctcgacccc IGG1 15 209 10 5 3 6 7 9 6 1 4 4 JY8QFUQ01A0QXW 3164 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 1065 100 214/214 nt 99.53 213/214 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-16*02 F 2 8 7 16 8.7.16 [1.17.38.11] CASANYDYVWGSYRYDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'A' (1 nucleotides at position 47 to 47 in the user submitted sequence), (cause frameshift); ctctggtggctccatcagtagttactactggagctggatccggcagAcccccagggaagggactggagtggattgggtatatctattacagtgggagcaccaactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgtgcctcagccaattatgattacgtttgggggagttatcgttatgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [295] g315>c|a316>t,R106>S(- - -)|g317>c,R106>S(- - -)|a320>c| 314 (320) 216 (222) 216 (218) 0 (4) 0 (2) 0 (2) 0 0 0 0 0 (1) 0 0 (1) 0 0 (2) 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 (8) 2 2 (4) 0 (4) 0 (2) 0 (2) 0 0 0 0 0 (1) 0 0 (1) 0 0 (2) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtggctccatcagtagttactac tggagctggatccggcagcccccagggaagggactggagtggattgggtat atctattacagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgt gcctcagccaattatgattacgtttgggggagttatcgttatgactac ASANYDYVWGSYRYDY IGHV4-59 IGHD3-16 IGHJ4 ggtggctccatcagtagttactac tggagctggatccggcagcccccagggaagggactggagtggattgggtat atctattacagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgt gcctcagccaattatgattacgtttgggggagttatcgttatgactac IGG1 0 210 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01A0RJS 9170 productive (see comment) Homsap IGHV3-7*01 F 927 92.17 200/217 nt 91.71 199/217 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-3*02 F 2 7 8 14 7.8.14 [1.17.38.11] CARGSYWHYDTFGDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 28 of V-REGION: 1 nucleotides (from position 11 in the user submitted sequence), (cause frameshift) cctctggattacttttaacaactattggatgacctgggttcgccaggctccagggaaggggctggaatgggtggccaacataaaacaacatggaggtgaaacgtactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgtgcgagagggtcgtattggcattatgataccttcggcgactattggggcccgggagtcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [291] c87>t|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|c126>t|g153>a|g171>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| c87>t|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|c126>t|g153>a| g171>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)| a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| a320>g| 319 (320) 224 (225) 207 17 (18) 6 (7) 11 3 (4) 5 2 1 2 0 0 0 3 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 19 4 1 3 0 2 1 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 0 1 0 0 0 30 24 18 6 2 4 2 2 0 0 1 0 0 0 1 0 0 0 117 114 110 4 1 3 1 0 0 0 1 0 0 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattacttttaacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccaac ataaaacaacatggaggtgaaacg tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat ARGSYWHYDTFGDY IGHV3-7 IGHD3-3 IGHJ4 ggattacttttaacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccaac ataaaacaacatggaggtgaaacg tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat IGG1 17 212 11 6 7 11 4 6 3 3 4 7 JY8QFUQ01A0S1H 17512 productive Homsap IGHV3-7*01 F 936 92.63 201/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-19*01 F 1 8 8 14 8.8.14 [1.17.38.11] CATDDSNGWDEVADYW in-frame + ctctggattcaccttcagctcccattggatgagctgggtccgccagactccagggaaggggctggagtgggtggccaacataaaggaagatggaagtgtgaagtattatgtggactctgtgaggggccgattcaccatctccagagacaacgccaagaattcattgtatctgcagatgaacagcctgagaggcgaggacacggctgtctattactgtgcgacagatgatagtaatggctgggacgaggtcgctgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagcagatgggaacgtggtcatcgcctgcctgtccagggcttcttccccca NA IGA1 71 95 [290, 289] t90>c|t105>c|a106>t|g107>c|t109>c,Y37>H(+ - -)|g133>a,A45>T(- - -)|c172>g,Q58>E(+ + -)|a191>t,E64>V(- + -)|a195>g|c198>t|a215>g,K72>R(+ + +)|c255>t|c259>t|a270>g|c287>g,A96>G(- + -)|g303>c|g317>c,R106>T(- - -)| t90>c|t105>c|a106>t|g107>c|t109>c,Y37>H(+ - -)| g133>a,A45>T(- - -)| c172>g,Q58>E(+ + -)|a191>t,E64>V(- + -)|a195>g| c198>t|a215>g,K72>R(+ + +)|c255>t|c259>t|a270>g|c287>g,A96>G(- + -)|g303>c| g317>c,R106>T(- - -)| 320 225 208 17 10 7 3 1 3 3 0 0 2 0 3 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 4 1 0 0 0 3 0 0 1 0 1 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 1 0 0 0 0 0 1 0 0 1 0 0 117 114 107 7 5 2 2 0 3 0 0 0 0 0 1 1 0 0 8 8 7 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagctcccattgg atgagctgggtccgccagactccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgtgaag tattatgtggactctgtgaggggccgattcaccatctccagagacaacgccaagaattcattgtatctgcagatgaacagcctgagaggcgaggacacggctgtctattactgt gcgacagatgatagtaatggctgggacgaggtcgctgactac ATDDSNGWDEVADY IGHV3-7 IGHD6-19 IGHJ4 ggattcaccttcagctcccattgg atgagctgggtccgccagactccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgtgaag tattatgtggactctgtgaggggccgattcaccatctccagagacaacgccaagaattcattgtatctgcagatgaacagcctgagaggcgaggacacggctgtctattactgt gcgacagatgatagtaatggctgggacgaggtcgctgactac IGA1 16 213 10 6 4 8 6 8 5 5 3 3 JY8QFUQ01A0TAV 14033 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 977 94.5 206/218 nt NA Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD6-25*01 F 2 8 8 15 8.8.15 [1.17.38.11] CVRRGMVAGRYDAFDIW in-frame + cctctggattcaccttcagtagttatagcatgaactgggtccgcctggctccagggaaggggctggagtgggtctcggccattagtattactagtagttccacatattacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagacctcactgtttctgcaaatgaacagcctgagagccgaggacacggctctgtattactgtgtgagaagggggatggtagcgggcagatatgatgcttttgatatctggggccgagggactatggtcaccgtctctccagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaagggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG2 100 100 [295] c108>t|a131>t,Q44>L(- - -)|a162>g|t163>g,S55>A(- + -)|g173>t,S58>I(- - -)|g176>c,S59>T(+ - +)|a191>c,Y64>S(+ - -)|t194>c,I65>T(- - -)|c198>t|a254>c,N85>T(- + -)|a263>t,Y88>F(- + -)|g301>c,V101>L(+ - +)|c314>t,A105>V(+ - +)| c108>t| a131>t,Q44>L(- - -)|a162>g|t163>g,S55>A(- + -)| g173>t,S58>I(- - -)|g176>c,S59>T(+ - +)|a191>c,Y64>S(+ - -)|t194>c,I65>T(- - -)| c198>t|a254>c,N85>T(- + -)|a263>t,Y88>F(- + -)|g301>c,V101>L(+ - +)| c314>t,A105>V(+ - +)|g319>a|a320>g| 318 (320) 224 (226) 211 13 (15) 3 (5) 10 1 (2) 0 (1) 3 1 2 0 2 0 2 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 48 3 1 2 1 0 0 0 0 0 1 0 0 0 0 1 30 24 20 4 0 4 0 0 0 1 1 0 0 0 1 0 1 0 117 114 110 4 1 3 0 0 1 0 1 0 1 0 1 0 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 (1) 0 (1) 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtagttatagc atgaactgggtccgcctggctccagggaaggggctggagtgggtctcggcc attagtattactagtagttccaca tattacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagacctcactgtttctgcaaatgaacagcctgagagccgaggacacggctctgtattactgt gtgagaagggggatggtagcgggcagatatgatgcttttgatatc VRRGMVAGRYDAFDI IGHV3-21 IGHD6-25 IGHJ3 ggattcaccttcagtagttatagc atgaactgggtccgcctggctccagggaaggggctggagtgggtctcggcc attagtattactagtagttccaca tattacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagacctcactgtttctgcaaatgaacagcctgagagccgaggacacggctctgtattactgt gtgagaagggggatggtagcgggcagatatgatgcttttgatatc IGG2 12 213 4 8 2 5 2 7 2 1 5 4 JY8QFUQ01A0TNI 17892 productive Homsap IGHV3-30*01 F 864 88.94 193/217 nt NA Homsap IGHJ6*02 F (see comment) 184 77.42 48/62 nt Homsap IGHD3-10*01 F 3 8 8 18 8.8.18 [1.17.38.11] CARGTMLRGVTKARYGLDVW in-frame - Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ctctggattcaccttcagtacctatgctatgtactgggtccgccaggctccaggcaaggggccagagtgggtgtcagtgatatcacatgatggaaataaggaagaatacgcagactccgtgaagggccgattcaccatttccagagacaactccaagaaaatgttgtacctgcaaatgaacaaccagcgacctgatgacacggctgtttattattgtgcgagagggactatgcttcggggagtcaccaaagcgagatacggtctggacgtctggggccaagggaccacggtcatcgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [303] g107>c,S36>T(+ - +)|c118>t,H40>Y(+ - -)|t149>c,L50>P(- - -)|g160>t,A54>S(- + -)|t165>g|t172>c,Y58>H(+ - -)|g188>a,S63>N(- - -)|t192>g,N64>K(+ - -)|a193>g,K65>E(+ - -)|t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|c234>t|t246>c|c255>a,N85>K(+ - -)|c257>t,T86>M(- - -)|c259>t|t264>c|g278>a,S93>N(- - -)|t281>a,L94>Q(- - -)|a283>c|g286>c,A96>P(- - -)|g291>t,E97>D(+ - +)|g303>t|c309>t| g107>c,S36>T(+ - +)| c118>t,H40>Y(+ - -)|t149>c,L50>P(- - -)|g160>t,A54>S(- + -)|t165>g| t172>c,Y58>H(+ - -)|g188>a,S63>N(- - -)|t192>g,N64>K(+ - -)|a193>g,K65>E(+ - -)| t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|c234>t|t246>c|c255>a,N85>K(+ - -)|c257>t,T86>M(- - -)|c259>t|t264>c|g278>a,S93>N(- - -)|t281>a,L94>Q(- - -)|a283>c|g286>c,A96>P(- - -)|g291>t,E97>D(+ - +)|g303>t|c309>t| a320>g| 319 (320) 224 (225) 200 24 (25) 8 (9) 16 1 (2) 2 5 4 1 2 0 1 2 0 3 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 47 4 1 3 0 0 1 1 0 0 0 0 0 0 1 1 30 24 20 4 0 4 1 1 0 1 0 0 0 0 0 0 0 1 117 114 99 15 7 8 0 1 4 2 1 2 0 1 1 0 2 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtacctatgct atgtactgggtccgccaggctccaggcaaggggccagagtgggtgtcagtg atatcacatgatggaaataaggaa gaatacgcagactccgtgaagggccgattcaccatttccagagacaactccaagaaaatgttgtacctgcaaatgaacaaccagcgacctgatgacacggctgtttattattgt gcgagagggactatgcttcggggagtcaccaaagcgagatacggtctggacgtc ARGTMLRGVTKARYGLDV IGHV3-30 IGHD3-10 IGHJ6 ggattcaccttcagtacctatgct atgtactgggtccgccaggctccaggcaaggggccagagtgggtgtcagtg atatcacatgatggaaataaggaa gaatacgcagactccgtgaagggccgattcaccatttccagagacaactccaagaaaatgttgtacctgcaaatgaacaaccagcgacctgatgacacggctgtttattattgt gcgagagggactatgcttcggggagtcaccaaagcgagatacggtctggacgtc IGG1 24 213 12 12 7 14 5 10 8 0 11 5 JY8QFUQ01A0UZS 9186 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 915 90.91 200/220 nt NA Homsap IGHJ4*02 F (see comment) 168 83.33 40/48 nt Homsap IGHD5-12*01 F 1 8 8 12 8.8.12 [2.17.38.11] CGKQLGAHKGADYW in-frame - Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) agcctctggattcacctttagtagttctggcatgtattgggcccgccaggctccagggaaggggctggagtgggtctcagctattagtggtagtggtgatgccacaaattacgcagactccgtgaagggccggttcaccatctccagagacaactccatgaacacactgtatctgcaaatgaacagcctgggaaccgatgacacggccttatattactgtggaaagcaattgggggcccataaaggggctgactactggggccaggggaccctggtcaccgtctcctctgcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] c105>t|c108>t|a110>c,Y37>S(+ - -)|c113>g,A38>G(- + -)|a118>t,S40>Y(+ - -)|g119>a,S40>Y(+ - -)|c120>t,S40>Y(+ - -)|t125>c,V42>A(+ - +)|g188>a,G63>D(- - -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|t246>c|a251>t,K84>M(- + -)|g258>a|a283>g,R95>G(- - -)|g286>a,A96>T(- - -)|g291>t,E97>D(+ - +)|g301>t,V101>L(+ - +)| c105>t|c108>t|a110>c,Y37>S(+ - -)|c113>g,A38>G(- + -)| a118>t,S40>Y(+ - -)|g119>a,S40>Y(+ - -)|c120>t,S40>Y(+ - -)|t125>c,V42>A(+ - +)| g188>a,G63>D(- - -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|t246>c|a251>t,K84>M(- + -)|g258>a|a283>g,R95>G(- - -)|g286>a,A96>T(- - -)|g291>t,E97>D(+ - +)|g301>t,V101>L(+ - +)| c314>g,A105>G(- + -)|g315>a,A105>G(- + -)|a318>g|g319>c| 313 (320) 221 (228) 201 (204) 20 (24) 4 (6) 16 (18) 2 (3) 4 (5) 4 2 1 0 2 1 1 (2) 1 (2) 2 0 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 0 2 0 1 0 0 0 0 1 0 0 51 51 47 4 0 4 0 1 1 1 0 0 1 0 0 0 0 0 30 24 21 3 0 3 1 1 0 0 0 0 0 0 1 0 0 0 117 114 105 9 2 7 1 2 1 1 0 0 1 1 0 0 2 0 1 (8) 1 1 (4) 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 0 (1) 0 (1) 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct ggattcacctttagtagttctggc atgtattgggcccgccaggctccagggaaggggctggagtgggtctcagct attagtggtagtggtgatgccaca aattacgcagactccgtgaagggccggttcaccatctccagagacaactccatgaacacactgtatctgcaaatgaacagcctgggaaccgatgacacggccttatattactgt ggaaagcaattgggggcccataaaggggctgactac GKQLGAHKGADY IGHV3-23 IGHD5-12 IGHJ4 ggattcacctttagtagttctggc atgtattgggcccgccaggctccagggaaggggctggagtgggtctcagct attagtggtagtggtgatgccaca aattacgcagactccgtgaagggccggttcaccatctccagagacaactccatgaacacactgtatctgcaaatgaacagcctgggaaccgatgacacggccttatattactgt ggaaagcaattgggggcccataaaggggctgactac IGA1 20 213 12 8 8 12 4 8 2 2 11 5 JY8QFUQ01A0VIE 12264 productive (see comment) Homsap IGHV3-30*01 F 878 89.45 195/218 nt 88.53 193/218 nt Homsap IGHJ3*01 F 205 90 45/50 nt Homsap IGHD2-2*02 F 2 8 8 11 8.8.11 [1.17.38.11] CACGHMRDGYDVW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 64 of V-REGION: 'CA' (2 nucleotides at position 99 to 100 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 83 of V-REGION: 'C' (1 nucleotides at position 156 to 156 in the user submitted sequence), (cause frameshift); cctctggattcaccttcagtgcctttactatgcactgggtccgccaggctccaggcgagggactagagtgggtggcagctatatcatatgatggcagtCAaaaaaatactatgcggactttgtgaagggccgattcaccatctccagagacaatcCccaagagtacactgtatctacaaatgaacggcctgggaggtgatgacacggctttgtattactgtgcgtgcggccatatgcgggatggttatgatgtctggggccaagggacaaaggtcaccgtctcttcaacatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [285] a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a110>t,Y37>F(- + -)|g112>a,A38>T(- - -)|a142>g,K48>E(+ - -)|g147>a|t164>c,V55>A(+ - +)|a186>c|t192>a,N64>K(+ - -)|c201>t|a204>g|c209>t,S70>F(- - -)|c210>t,S70>F(- - -)|t247>c,S83>P(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|g267>a|a277>g,S93>G(+ + -)|a283>g,R95>G(- - -)|c287>g,A96>G(- + -)|g291>t,E97>D(+ - +)|g301>t,V101>L(+ - +)| a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a110>t,Y37>F(- + -)|g112>a,A38>T(- - -)| a142>g,K48>E(+ - -)|g147>a|t164>c,V55>A(+ - +)| a186>c|t192>a,N64>K(+ - -)| c201>t|a204>g|c209>t,S70>F(- - -)|c210>t,S70>F(- - -)|t247>c,S83>P(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|g267>a|a277>g,S93>G(+ + -)|a283>g,R95>G(- - -)|c287>g,A96>G(- + -)|g291>t,E97>D(+ - +)|g301>t,V101>L(+ - +)| a316>t,R106>C(- - -)|a318>c,R106>C(- - -)|a320>g| 315 (320) 221 (226) 198 (200) 23 (26) 6 (7) 17 (19) 6 (7) 4 4 2 1 (2) 0 1 (2) 1 1 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 0 0 0 0 1 0 1 0 0 0 51 51 48 3 1 2 1 1 0 1 0 0 0 0 0 0 0 0 30 24 22 2 1 1 0 0 0 0 1 0 0 1 0 0 0 0 117 114 100 14 4 10 4 2 4 1 0 0 0 0 0 1 2 0 3 (8) 3 3 (5) 0 (3) 0 (1) 0 (2) 0 (1) 0 0 0 0 (1) 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtgcctttact atgcactgggtccgccaggctccaggcgagggactagagtgggtggcagct atatcatatgatggcagtaaaaaa tactatgcggactttgtgaagggccgattcaccatctccagagacaatcccaagagtacactgtatctacaaatgaacggcctgggaggtgatgacacggctttgtattactgt gcgtgcggccatatgcgggatggttatgatgtc ACGHMRDGYDV IGHV3-30 IGHD2-2 IGHJ3 ggattcaccttcagtgcctttact atgcactgggtccgccaggctccaggcgagggactagagtgggtggcagct atatcatatgatggcagtaaaaaa tactatgcggactttgtgaagggccgattcaccatctccagagacaatcccaagagtacactgtatctacaaatgaacggcctgggaggtgatgacacggctttgtattactgt gcgtgcggccatatgcgggatggttatgatgtc IGA1 23 213 16 7 8 12 8 11 5 1 12 5 JY8QFUQ01A0WDV 20776 productive Homsap IGHV3-66*02 F 903 91.59 196/214 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-15*01 F 3 8 7 16 8.7.16 [1.17.38.11] CAKLADTGEWSNRIFYFW in-frame + ctctggattcaccgtcagtagcagcttcatgacttgggtccgccaggctccaggaaagggactggagtgggtctcagtgctttatgtcggtggtaacacatactacgcagactccgtgaagggccgattcaccacctccagagacaattccgagaacactctgtatcttcaaatgaacaacctgagacctgaggactcggctgtgtattattgtgcgaaattggcggatactggagagtggagcaaccgcatcttttatttctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [294] a110>g,N37>S(- - -)|a113>t,Y38>F(- + -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g141>a|g147>a|t165>g|a166>c,I56>L(+ + +)|a172>g,S58>V(- - -)|g173>t,S58>V(- - -)|g191>a,S64>N(- - -)|t233>c,I78>T(- - -)|a250>g,K84>E(+ - -)|g258>t|g278>a,S93>N(- - -)|g286>c,A96>P(- - -)|a295>t,T99>S(+ - +)|c309>t| a110>g,N37>S(- - -)|a113>t,Y38>F(- + -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g141>a|g147>a|t165>g| a166>c,I56>L(+ + +)|a172>g,S58>V(- - -)|g173>t,S58>V(- - -)|g191>a,S64>N(- - -)| t233>c,I78>T(- - -)|a250>g,K84>E(+ - -)|g258>t|g278>a,S93>N(- - -)|g286>c,A96>P(- - -)|a295>t,T99>S(+ - +)|c309>t| g317>a,R106>K(+ + +)| 316 (318) 218 (220) 200 (201) 18 (19) 5 13 (14) 3 4 (5) 2 1 1 0 2 0 2 0 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 51 51 46 5 3 2 0 2 1 0 0 0 0 0 1 0 0 1 30 21 17 4 0 4 1 1 0 0 1 0 0 0 0 0 1 0 117 114 107 7 2 5 1 1 1 1 0 0 1 0 1 0 1 0 4 (6) 4 4 (5) 0 (1) 0 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccgtcagtagcagcttc atgacttgggtccgccaggctccaggaaagggactggagtgggtctcagtg ctttatgtcggtggtaacaca tactacgcagactccgtgaagggccgattcaccacctccagagacaattccgagaacactctgtatcttcaaatgaacaacctgagacctgaggactcggctgtgtattattgt gcgaaattggcggatactggagagtggagcaaccgcatcttttatttc AKLADTGEWSNRIFYF IGHV3-66 IGHD2-15 IGHJ4 ggattcaccgtcagtagcagcttc atgacttgggtccgccaggctccaggaaagggactggagtgggtctcagtg ctttatgtcggtggtaacaca tactacgcagactccgtgaagggccgattcaccacctccagagacaattccgagaacactctgtatcttcaaatgaacaacctgagacctgaggactcggctgtgtattattgt gcgaaattggcggatactggagagtggagcaaccgcatcttttatttc IGG2 18 210 10 8 6 10 4 8 5 0 7 6 JY8QFUQ01A0WZB 5136 productive Homsap IGHV3-66*02 F (see comment) 759 84.11 180/214 nt NA Homsap IGHJ6*02 F (see comment) 197 80.33 49/61 nt Homsap IGHD2-21*01 F 3 8 7 11 8.7.11 [1.17.38.11] CARHLMVYALDVW in-frame + Low V-REGION identity (84,11% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ctctggattcaccgtcagtgggaagtatatgagttgggtccgccaggctccaggcaaggggctggagtgggtctcagtcttatttagtactggcactgcatactacgcagactccgtgaaaggccggttcaccatctccagagacaattccaacaacaccctatatcttcagatgaacaacatcagacctgaagacgcggccacttattattgtgcgaggcatttaatggtctacgctctggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [278] a106>g,S36>G(+ + -)|c108>g,S36>G(+ + -)|c111>g,N37>K(+ - -)|c114>t|c120>t|g141>c|t165>c|a166>t,I56>L(+ + +)|t168>a,I56>L(+ + +)|a170>t,Y57>F(- + -)|c174>t|g175>a,G59>T(+ - -)|g176>c,G59>T(+ - -)|t189>c|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|a193>g,T65>A(- - -)|g216>a|a225>g|g252>c,K84>N(+ - -)|g258>c|g261>a|a270>g|g278>a,S93>N(- - -)|c280>a,L94>I(+ + +)|g282>c,L94>I(+ + +)|g286>c,A96>P(- - -)|g291>a|a295>g,T99>A(- - -)|t300>c|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|c309>t| a106>g,S36>G(+ + -)|c108>g,S36>G(+ + -)|c111>g,N37>K(+ - -)|c114>t| c120>t|g141>c|t165>c| a166>t,I56>L(+ + +)|t168>a,I56>L(+ + +)|a170>t,Y57>F(- + -)|c174>t|g175>a,G59>T(+ - -)|g176>c,G59>T(+ - -)|t189>c|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|a193>g,T65>A(- - -)| g216>a|a225>g|g252>c,K84>N(+ - -)|g258>c|g261>a|a270>g|g278>a,S93>N(- - -)|c280>a,L94>I(+ + +)|g282>c,L94>I(+ + +)|g286>c,A96>P(- - -)|g291>a|a295>g,T99>A(- - -)|t300>c|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|c309>t| a318>g| 317 (318) 219 (220) 185 34 (35) 14 (15) 20 5 (6) 6 5 4 0 1 2 1 7 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 1 0 0 0 0 0 0 2 0 0 51 51 48 3 3 0 0 0 1 1 0 0 0 0 1 0 0 0 30 21 11 10 2 8 1 1 2 1 0 0 2 1 2 0 0 0 117 114 97 17 8 9 3 5 1 2 0 1 0 0 4 0 1 0 5 (6) 5 5 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccgtcagtgggaagtat atgagttgggtccgccaggctccaggcaaggggctggagtgggtctcagtc ttatttagtactggcactgca tactacgcagactccgtgaaaggccggttcaccatctccagagacaattccaacaacaccctatatcttcagatgaacaacatcagacctgaagacgcggccacttattattgt gcgaggcatttaatggtctacgctctggacgtc ARHLMVYALDV IGHV3-66 IGHD2-21 IGHJ6 ggattcaccgtcagtgggaagtat atgagttgggtccgccaggctccaggcaaggggctggagtgggtctcagtc ttatttagtactggcactgca tactacgcagactccgtgaaaggccggttcaccatctccagagacaattccaacaacaccctatatcttcagatgaacaacatcagacctgaagacgcggccacttattattgt gcgaggcatttaatggtctacgctctggacgtc IGA1 34 210 20 14 11 22 9 12 11 3 9 11 JY8QFUQ01A0X8W 8339 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 1000 95.43 209/219 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-10*01 F 2 8 8 15 8.8.15 [2.17.38.11] CARDSRHSASGSYSDYW in-frame - gcgtctggattcaccttcaatagccatggcatgcactgggtccgccaggcgccaggcaaggggctggagtgggtggctgctatttggtttgatggaagtaataaatactatgcagactccgtgaagggacgattcaccatctccagagacaattccaagaacacgttgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtactactgtgcgagagattcccggcactctgcctcggggagttattccgactactggggccagggaaccctggtcgccgtttcttcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [296] g104>a,S35>N(- - -)|t109>c,Y37>H(+ - -)|t135>g|a162>t|t164>c,V55>A(+ - +)|a168>t|a173>t,Y58>F(- + -)|c222>a|c259>t|t306>c| g104>a,S35>N(- - -)|t109>c,Y37>H(+ - -)| t135>g|a162>t|t164>c,V55>A(+ - +)| a168>t|a173>t,Y58>F(- + -)| c222>a|c259>t|t306>c| 320 227 217 10 6 4 0 1 1 3 0 1 3 0 0 0 0 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 0 0 1 0 0 1 0 0 0 0 1 30 24 22 2 1 1 0 0 0 0 0 0 2 0 0 0 0 0 117 114 111 3 3 0 0 0 1 1 0 1 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcgtct ggattcaccttcaatagccatggc atgcactgggtccgccaggcgccaggcaaggggctggagtgggtggctgct atttggtttgatggaagtaataaa tactatgcagactccgtgaagggacgattcaccatctccagagacaattccaagaacacgttgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtactactgt gcgagagattcccggcactctgcctcggggagttattccgactac ARDSRHSASGSYSDY IGHV3-33 IGHD3-10 IGHJ4 ggattcaccttcaatagccatggc atgcactgggtccgccaggcgccaggcaaggggctggagtgggtggctgct atttggtttgatggaagtaataaa tactatgcagactccgtgaagggacgattcaccatctccagagacaattccaagaacacgttgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtactactgt gcgagagattcccggcactctgcctcggggagttattccgactac IGG1 10 213 5 5 2 3 3 7 5 1 1 3 JY8QFUQ01A0XE3 6907 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 818 86.98 187/215 nt 86.05 185/215 nt Homsap IGHJ3*01 F 169 82 41/50 nt Homsap IGHD3-16*01 F 3 8 7 12 8.7.12 [1.17.37.11] CARDDGGHMTLDVW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 62 of V-REGION: 1 nucleotides (from position 94 in the user submitted sequence), (cause frameshift)in FR3-IMGT, from codon 84 of V-REGION: 1 nucleotides (from position 154 in the user submitted sequence), (cause frameshift) gtctggattcacatttagaagctattccatgaattgggtccgccaggctccagggaaggggctggagtgggtctcagctgtcagtggtggtggggcgccacaaactacgcggagtccgtgaagggccggttcaccatctccagagacaattccaggggacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgtgcgagagatgacggcggacacatgactttagatgtctggggccaagggacaatggtcagcgtctctccggcctccaccaagggcccatcggtcttcccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcagg NA IGG1 100 94 [282, 283] c75>g|c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a175>g,S59>G(+ + -)|t189>c|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|t196>a,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|c287>t,A96>V(+ - +)|g291>a|g297>a|g301>t,V101>L(+ - +)|c309>t|a317>g,K106>R(+ + +)| c75>g| c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a175>g,S59>G(+ + -)|t189>c|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| t196>a,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|c287>t,A96>V(+ - +)|g291>a|g297>a|g301>t,V101>L(+ - +)|c309>t| a317>g,K106>R(+ + +)| 320 223 194 29 9 20 7 4 4 3 0 2 1 1 1 4 2 0 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 21 3 1 2 0 0 0 0 0 2 0 0 0 0 1 0 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 23 17 6 1 5 3 0 0 2 0 0 0 0 1 0 0 0 117 113 97 16 6 10 3 3 3 1 0 0 1 1 0 3 1 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagct gtcagtggtggtggggcgccaca aactacgcggagtccgtgaagggccggttcaccatctccagagacaattccaggggacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgt gcgagagatgacggcggacacatgactttagatgtc ARDDGGHMTLDV IGHV3-23 IGHD3-16 IGHJ3 ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagct gtcagtggtggtggggcgccaca aactacgcggagtccgtgaagggccggttcaccatctccagagacaattccaggggacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgt gcgagagatgacggcggacacatgactttagatgtc IGG1 27 211 17 10 8 16 9 11 6 2 12 7 JY8QFUQ01A0Z64 19665 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 999 95.85 208/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD3-10*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKKYHYGSGSYLYCFDYW in-frame - ctctggattcacctttagcaactttgccatgacctgggtccgccaggctccagggaagggactggagtgggtctcaactattagtggtggtgatgatagcacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatatcactgtgcgaagaagtatcactatggttcggggagttatctctactgctttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [299] g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)|g119>c,S40>T(+ - +)|g147>a|g163>a,A55>T(- - -)|a175>g,S59>G(+ + -)|g185>a,G62>D(- - -)|g188>a,G63>D(- - -)|t307>c,Y103>H(+ - -)| g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)| g119>c,S40>T(+ - +)|g147>a|g163>a,A55>T(- - -)| a175>g,S59>G(+ + -)|g185>a,G62>D(- - -)|g188>a,G63>D(- - -)| t307>c,Y103>H(+ - -)| a318>g|g319>a| 317 (320) 222 (225) 213 (214) 9 (11) 1 (3) 8 1 (2) 5 (6) 0 1 0 0 1 0 1 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 1 0 0 0 0 0 51 51 48 3 1 2 0 2 0 0 0 0 0 0 1 0 0 0 30 24 21 3 0 3 1 2 0 0 0 0 0 0 0 0 0 0 117 114 113 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcaactttgcc atgacctgggtccgccaggctccagggaagggactggagtgggtctcaact attagtggtggtgatgatagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatatcactgt gcgaagaagtatcactatggttcggggagttatctctactgctttgactac AKKYHYGSGSYLYCFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcacctttagcaactttgcc atgacctgggtccgccaggctccagggaagggactggagtgggtctcaact attagtggtggtgatgatagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatatcactgt gcgaagaagtatcactatggttcggggagttatctctactgctttgactac IGA2 9 213 7 2 5 6 2 3 1 0 3 5 JY8QFUQ01A0ZW5 18098 productive Homsap IGHV4-61*02 F 857 87.78 194/221 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-19*01 F 1 10 7 11 10.7.11 [1.17.38.11] CARSGWDNQFDFW in-frame + tctctggtgtcgccaccagtagtggcacttactactggagctggatccggcagtccgccggggcgggactagagtggattgggcgcatctataccggtcacaccaccatttacaacccctccctcaagggtcgagtcaccatgtcacttgacatgtccaagaaccagatctccctgaggctgacctctgtgaccgccgcagatacggccgtgtattactgtgcgcgaagtggctgggacaatcaatttgacttctggggccagggaaccctggtcaccgtctcctcagcttccaccaagggcccatccgtcttcccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG4 100 88 [287, 286] g83>t,G28>V(- - -)|t85>g,S29>A(- + -)|t89>c,I30>T(- - -)|c93>t|t105>c|g107>c,S36>T(+ - +)|c133>t,P45>S(+ - -)|a142>g,K48>A(- - -)|a143>c,K48>A(- - -)|g150>a|t165>c|a175>g,S59>G(+ + -)|g187>c,G63>H(+ - -)|g188>a,G63>H(+ - -)|g189>c,G63>H(+ - -)|g191>c,S64>T(+ - +)|a197>t,N66>I(- - -)|c198>t,N66>I(- - -)|a220>g,S74>G(+ + -)|a234>g,I78>M(+ + -)|g238>c,V80>L(+ - +)|a240>t,V80>L(+ - +)|c245>t,T82>M(- - -)|t259>a,F87>I(+ - -)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c294>t|a316>c| g83>t,G28>V(- - -)|t85>g,S29>A(- + -)|t89>c,I30>T(- - -)|c93>t|t105>c|g107>c,S36>T(+ - +)| c133>t,P45>S(+ - -)|a142>g,K48>A(- - -)|a143>c,K48>A(- - -)|g150>a|t165>c| a175>g,S59>G(+ + -)|g187>c,G63>H(+ - -)|g188>a,G63>H(+ - -)|g189>c,G63>H(+ - -)|g191>c,S64>T(+ - +)| a197>t,N66>I(- - -)|c198>t,N66>I(- - -)|a220>g,S74>G(+ + -)|a234>g,I78>M(+ + -)|g238>c,V80>L(+ - +)|a240>t,V80>L(+ - +)|c245>t,T82>M(- - -)|t259>a,F87>I(+ - -)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c294>t| a316>c|g319>a|a320>g| 318 (320) 227 (229) 199 28 (30) 6 (8) 22 5 (6) 2 (3) 5 3 2 0 2 1 6 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 2 4 0 0 1 2 0 0 0 0 1 0 1 1 51 51 46 5 2 3 1 1 1 1 1 0 0 0 0 0 0 0 30 21 16 5 0 5 1 1 0 0 0 0 0 0 3 0 0 0 117 114 103 11 1 10 3 0 3 0 0 0 2 1 2 0 0 0 6 (8) 6 5 1 (3) 1 (3) 0 0 (1) 0 (1) 0 0 1 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgtcgccaccagtagtggcacttactac tggagctggatccggcagtccgccggggcgggactagagtggattgggcgc atctataccggtcacaccacc atttacaacccctccctcaagggtcgagtcaccatgtcacttgacatgtccaagaaccagatctccctgaggctgacctctgtgaccgccgcagatacggccgtgtattactgt gcgcgaagtggctgggacaatcaatttgacttc ARSGWDNQFDF IGHV4-61 IGHD6-19 IGHJ4 ggtgtcgccaccagtagtggcacttactac tggagctggatccggcagtccgccggggcgggactagagtggattgggcgc atctataccggtcacaccacc atttacaacccctccctcaagggtcgagtcaccatgtcacttgacatgtccaagaaccagatctccctgaggctgacctctgtgaccgccgcagatacggccgtgtattactgt gcgcgaagtggctgggacaatcaatttgacttc IGG4 27 216 15 12 7 14 8 13 3 2 13 9 JY8QFUQ01A0ZX6 14509 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD4-17*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAGHLLGDPFDYW in-frame + ttctggagacaactttagcagatactggatcggctgggtccgccagatgcccgggaaaggcctggagtggatggggatcatctatcctggtgactctgacaccagatacagtccgtccttccaaggccaggtcaccatctcagccgacaagtccaccagtaccgcctacctgcagtggagcagtctgaaggtctcggacaccgccacgtattactgtgcgggacatcttctcggtgacccttttgactactggggccagggaaccccggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggcacagcggccctggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggcaactcag NA IGG1 79 94 [282] t82>g,Y28>D(- - -)|g86>a,S29>N(- - -)|c104>g,T35>S(+ - +)|c108>a,S36>R(- - -)|g126>c|t192>c|c204>t|t251>c,I84>T(- - -)|c255>t|c279>t|c287>t,A96>V(+ - +)|t302>c,M101>T(- - -)|a316>g,R106>G(- - -)| t82>g,Y28>D(- - -)|g86>a,S29>N(- - -)|c104>g,T35>S(+ - +)|c108>a,S36>R(- - -)| g126>c| t192>c| c204>t|t251>c,I84>T(- - -)|c255>t|c279>t|c287>t,A96>V(+ - +)|t302>c,M101>T(- - -)| a316>g,R106>G(- - -)| 320 225 212 13 5 8 1 1 4 3 0 1 0 0 1 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 0 0 0 1 0 0 0 1 0 1 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 108 6 3 3 0 0 4 2 0 0 0 0 0 0 0 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ttct ggagacaactttagcagatactgg atcggctgggtccgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgacacc agatacagtccgtccttccaaggccaggtcaccatctcagccgacaagtccaccagtaccgcctacctgcagtggagcagtctgaaggtctcggacaccgccacgtattactgt gcgggacatcttctcggtgacccttttgactac AGHLLGDPFDY IGHV5-51 IGHD4-17 IGHJ4 ggagacaactttagcagatactgg atcggctgggtccgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgacacc agatacagtccgtccttccaaggccaggtcaccatctcagccgacaagtccaccagtaccgcctacctgcagtggagcagtctgaaggtctcggacaccgccacgtattactgt gcgggacatcttctcggtgacccttttgactac IGG1 12 213 8 4 5 8 3 4 4 1 3 4 JY8QFUQ01A110D 7647 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 964 93.61 205/219 nt NA Homsap IGHJ5*02 F 201 88.24 45/51 nt Homsap IGHD6-13*01 F 2 8 8 16 8.8.16 [2.17.38.11] CARGRIVAVGMSGWFDPW in-frame - gcctctggattcaccttcagtaactactggatgtattgggtccgccaagctccagggaaggggctggtgtgggtctcacgtattaatggtgatggcagtagcacaagctacggggactccgtgaagggccgattcaccgtctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgcgagtcgacgacacggctctatattattgtgcaagaggccgtatagtagcagttgggatgtcaggctggttcgacccttggggccagggaaccctggtctccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [298] g107>a,S36>N(- - -)|c118>t,H40>Y(+ - -)|c120>t,H40>Y(+ - -)|a172>g,S58>G(+ + -)|g186>c|c203>g,A68>G(- + -)|a232>g,I78>V(+ - +)|g258>c|a283>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)|c309>t| g107>a,S36>N(- - -)| c118>t,H40>Y(+ - -)|c120>t,H40>Y(+ - -)| a172>g,S58>G(+ + -)|g186>c| c203>g,A68>G(- + -)|a232>g,I78>V(+ - +)|g258>c|a283>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)|c309>t| a320>g| 319 (320) 226 (227) 212 14 (15) 4 (5) 10 2 (3) 2 4 0 1 0 0 0 4 1 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 2 0 0 0 0 0 0 0 0 0 30 24 22 2 1 1 1 0 0 0 0 0 0 0 1 0 0 0 117 114 105 9 3 6 1 1 2 0 1 0 0 0 3 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcaccttcagtaactactgg atgtattgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attaatggtgatggcagtagcaca agctacggggactccgtgaagggccgattcaccgtctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgcgagtcgacgacacggctctatattattgt gcaagaggccgtatagtagcagttgggatgtcaggctggttcgaccct ARGRIVAVGMSGWFDP IGHV3-74 IGHD6-13 IGHJ5 ggattcaccttcagtaactactgg atgtattgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attaatggtgatggcagtagcaca agctacggggactccgtgaagggccgattcaccgtctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgcgagtcgacgacacggctctatattattgt gcaagaggccgtatagtagcagttgggatgtcaggctggttcgaccct IGA1 14 213 8 6 6 11 2 3 3 1 8 2 JY8QFUQ01A12BY 20463 productive (see comment) Homsap IGHV3-7*01 F 959 93.58 204/218 nt 92.2 201/218 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARETLPLGVGGFDNW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'G' (1 nucleotides at position 11 to 11 in the user submitted sequence), (cause frameshift);in CDR1-IMGT at codon 36 of V-REGION: 'G' (1 nucleotides at position 22 to 22 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 46 of V-REGION: 'G' (1 nucleotides at position 55 to 55 in the user submitted sequence), (cause frameshift); cctctggattGcacatttagtGaattattggatgatctgggtccgccaggctccGagggaaggggctggagtgggtggccaacataaaccaagatggaggtgacatggcctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactctctgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgtgcgagagagacactcccccttggtgttgggggttttgacaactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [295] c87>a|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g119>t,S40>I(- - -)|g171>c,K57>N(+ - -)|a187>g,S63>G(+ + -)|g192>c,E64>D(+ - +)|a194>t,K65>M(- + -)|a195>g,K65>M(- + -)|t196>g,Y66>A(- - -)|a197>c,Y66>A(- - -)|a258>t|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| c87>a|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g119>t,S40>I(- - -)| g171>c,K57>N(+ - -)|a187>g,S63>G(+ + -)|g192>c,E64>D(+ - +)|a194>t,K65>M(- + -)|a195>g,K65>M(- + -)| t196>g,Y66>A(- - -)|a197>c,Y66>A(- - -)|a258>t|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 226 212 14 2 12 2 3 1 0 1 1 2 0 2 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 0 0 1 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 30 24 19 5 0 5 2 0 0 0 0 0 1 0 2 0 0 0 117 114 109 5 1 4 0 2 0 0 1 0 1 0 0 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacatttagtaattattgg atgatctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaccaagatggaggtgacatg gcctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactctctgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgt gcgagagagacactcccccttggtgttgggggttttgacaac ARETLPLGVGGFDN IGHV3-7 IGHD3-10 IGHJ4 ggattcacatttagtaattattgg atgatctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaccaagatggaggtgacatg gcctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactctctgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgt gcgagagagacactcccccttggtgttgggggttttgacaac IGG1 14 213 6 8 4 8 2 6 1 1 5 7 JY8QFUQ01A12KV 8555 productive Homsap IGHV3-48*01 F 815 86.24 188/218 nt NA Homsap IGHJ6*02 F 196 88 44/50 nt Homsap IGHD6-6*01 F 1 8 8 8 8.8.8 [1.17.38.11] CVRGAGLDVW in-frame + cctcagggttcagtttcaacaactataacatggcctgggtccgccagactccagggaaggggctggagtgtgtggcatatattagtagtagaagtagtaacagatattacacagcctctgtggagggccgattcaccatctccagagacaatgccaggaattctctgtatctccaaatgaatggcctgagagccgacgacacggctgtatattactgtgtgagaggcgcgggtctggacgtctggggccaagggaccacggtcgccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [274] t78>a|a81>g|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)|g113>a,S38>N(- - -)|a118>g,N40>A(- - -)|a119>c,N40>A(- - -)|g133>a,A45>T(- - -)|g156>t,W52>C(+ - -)|t159>g|t160>g,S54>A(- + -)|c165>t|t177>a,S59>R(- - -)|c191>a,T64>N(- + -)|t194>g,I65>R(- + -)|c198>t|g202>a,A68>T(- - -)|a206>c,D69>A(- - -)|a214>g,K72>E(+ - -)|a251>g,K84>R(+ + +)|c255>t|a258>t|g267>c|c276>t|a277>g,S93>G(+ + -)|g291>c,E97>D(+ - +)|g303>a|c314>t,A105>V(+ - +)| t78>a| a81>g|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)|g113>a,S38>N(- - -)| a118>g,N40>A(- - -)|a119>c,N40>A(- - -)|g133>a,A45>T(- - -)|g156>t,W52>C(+ - -)|t159>g|t160>g,S54>A(- + -)|c165>t| t177>a,S59>R(- - -)|c191>a,T64>N(- + -)|t194>g,I65>R(- + -)| c198>t|g202>a,A68>T(- - -)|a206>c,D69>A(- - -)|a214>g,K72>E(+ - -)|a251>g,K84>R(+ + +)|c255>t|a258>t|g267>c|c276>t|a277>g,S93>G(+ + -)|g291>c,E97>D(+ - +)|g303>a| c314>t,A105>V(+ - +)|a320>g| 319 (320) 225 (226) 194 31 (32) 10 (11) 21 5 (6) 6 6 1 2 1 1 2 2 1 1 3 78 5 4 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 17 7 1 6 1 3 1 1 0 0 0 0 0 1 0 0 51 51 44 7 2 5 1 1 1 0 1 0 0 0 0 0 1 2 30 24 21 3 0 3 0 0 0 0 0 1 0 1 0 0 0 1 117 114 102 12 6 6 3 2 3 0 1 0 1 0 2 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctca gggttcagtttcaacaactataac atggcctgggtccgccagactccagggaaggggctggagtgtgtggcatat attagtagtagaagtagtaacaga tattacacagcctctgtggagggccgattcaccatctccagagacaatgccaggaattctctgtatctccaaatgaatggcctgagagccgacgacacggctgtatattactgt gtgagaggcgcgggtctggacgtc VRGAGLDV IGHV3-48 IGHD6-6 IGHJ6 gggttcagtttcaacaactataac atggcctgggtccgccagactccagggaaggggctggagtgtgtggcatat attagtagtagaagtagtaacaga tattacacagcctctgtggagggccgattcaccatctccagagacaatgccaggaattctctgtatctccaaatgaatggcctgagagccgacgacacggctgtatattactgt gtgagaggcgcgggtctggacgtc IGG1 29 213 17 12 11 16 6 13 8 1 11 9 JY8QFUQ01A12V0 5680 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 819 86.64 188/217 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-16*02 F 2 8 8 9 8.8.9 [1.17.38.11] CTGLTYPDDYW in-frame + gtctggatttaccttcagtaagttctggatgcattgggtccgccaagctccagggaaggggctgacttgggtctcacgtattaatcctgatgggactatcacgaactacacggactccgtgaggggccgattcatcacttccagagacaacgccaagaacacagtatatctgcagatgaacagtctgcgagtcgaggacacaggtgtatattactgtacaggactgacttatccagacgactactggggccagggatgtttggccaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [275] c75>g|c84>t|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a110>t,Y37>F(- + -)|c120>t|g151>a,V51>T(- - -)|t152>c,V51>T(- - -)|g153>t,V51>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g188>c,S63>T(+ - +)|g191>t,S64>I(- - -)|a195>g|g197>a,S66>N(- - -)|g202>a,A68>T(- - -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|t233>c,I78>T(- - -)|c234>t,I78>T(- - -)|g258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a270>g|a283>c|c287>t,A96>V(+ - +)|g297>a|c299>g,A100>G(- + -)|g303>a|g313>a,A105>T(- - -)|a316>g,R106>G(- - -)| c75>g| c84>t|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a110>t,Y37>F(- + -)| c120>t|g151>a,V51>T(- - -)|t152>c,V51>T(- - -)|g153>t,V51>T(- - -)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g188>c,S63>T(+ - +)|g191>t,S64>I(- - -)|a195>g| g197>a,S66>N(- - -)|g202>a,A68>T(- - -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|t233>c,I78>T(- - -)|c234>t,I78>T(- - -)|g258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a270>g|a283>c|c287>t,A96>V(+ - +)|g297>a|c299>g,A100>G(- + -)|g303>a| g313>a,A105>T(- - -)|a316>g,R106>G(- - -)|g319>c|a320>t| 318 (320) 223 (225) 192 31 (33) 9 (11) 22 4 9 5 2 2 0 1 (2) 0 2 (3) 4 2 0 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 20 4 1 3 0 1 1 0 0 0 1 0 0 1 0 0 51 51 47 4 1 3 0 1 1 1 0 0 0 0 0 0 1 0 30 24 19 5 1 4 1 0 0 0 1 0 0 0 2 0 1 0 117 114 99 15 5 10 2 6 3 1 1 0 0 0 0 2 0 0 6 (8) 6 4 2 (4) 0 (2) 2 1 1 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggatttaccttcagtaagttctgg atgcattgggtccgccaagctccagggaaggggctgacttgggtctcacgt attaatcctgatgggactatcacg aactacacggactccgtgaggggccgattcatcacttccagagacaacgccaagaacacagtatatctgcagatgaacagtctgcgagtcgaggacacaggtgtatattactgt acaggactgacttatccagacgactac TGLTYPDDY IGHV3-74 IGHD3-16 IGHJ4 ggatttaccttcagtaagttctgg atgcattgggtccgccaagctccagggaaggggctgacttgggtctcacgt attaatcctgatgggactatcacg aactacacggactccgtgaggggccgattcatcacttccagagacaacgccaagaacacagtatatctgcagatgaacagtctgcgagtcgaggacacaggtgtatattactgt acaggactgacttatccagacgactac IGA1 28 213 18 10 13 20 5 8 6 2 13 7 JY8QFUQ01A14EE 9926 productive Homsap IGHV3-48*02 F, or Homsap IGHV3-48*04 F 815 86.24 188/218 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-21*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAKVGNLYFFEYW in-frame + cctctggattcagcttcaacagctacagcatgaactgggtccgccaggctccagggaagggactggaatggatctcatcaattagtaccgctggcaccaccataggctacgcagactctgtgaagggccgattcactatttccagagacaacgccaagaactcagtatctctgcagatggacagcctgagagacgaggacacggcggtatattactgtgccaaagtgggtaatttgtatttctttgaatactggggccggggaaccctggtcaccgtctcctcagcatcccgaccagcccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 89 [280] c86>g,T29>S(+ - +)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|t111>c|g147>a|g153>a|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|a164>c,Y55>S(+ - -)|c165>a,Y55>S(+ - -)|g173>c,S58>T(+ - +)|t174>c,S58>T(+ - +)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|a184>g,S62>G(+ + -)|t186>c,S62>G(+ + -)|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)|t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c231>t|c234>t|t246>c|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a263>c,Y88>S(+ - -)|a270>g|a274>g,N92>D(+ + -)|t300>g|g303>a|g315>c|g317>a,R106>K(+ + +)| c86>g,T29>S(+ - +)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|t111>c| g147>a|g153>a|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|a164>c,Y55>S(+ - -)|c165>a,Y55>S(+ - -)| g173>c,S58>T(+ - +)|t174>c,S58>T(+ - +)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|a184>g,S62>G(+ + -)|t186>c,S62>G(+ + -)|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)| t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c231>t|c234>t|t246>c|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a263>c,Y88>S(+ - -)|a270>g|a274>g,N92>D(+ + -)|t300>g|g303>a| g315>c|g317>a,R106>K(+ + +)|a320>t| 319 (320) 225 (226) 193 32 (33) 10 (11) 22 5 7 2 7 2 1 0 (1) 0 4 2 0 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 0 2 0 0 0 0 0 1 0 0 51 51 45 6 2 4 0 3 0 1 1 1 0 0 0 0 0 0 30 24 16 8 0 8 2 0 0 3 0 0 0 0 3 0 0 0 117 114 102 12 6 6 3 2 2 1 1 0 0 0 0 1 0 2 7 (8) 7 5 2 (3) 1 (2) 1 0 1 0 0 0 0 0 (1) 0 1 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| cctct ggattcagcttcaacagctacagc atgaactgggtccgccaggctccagggaagggactggaatggatctcatca attagtaccgctggcaccaccata ggctacgcagactctgtgaagggccgattcactatttccagagacaacgccaagaactcagtatctctgcagatggacagcctgagagacgaggacacggcggtatattactgt gccaaagtgggtaatttgtatttctttgaatac AKVGNLYFFEY IGHV3-48 IGHD2-21 IGHJ4 ggattcagcttcaacagctacagc atgaactgggtccgccaggctccagggaagggactggaatggatctcatca attagtaccgctggcaccaccata ggctacgcagactctgtgaagggccgattcactatttccagagacaacgccaagaactcagtatctctgcagatggacagcctgagagacgaggacacggcggtatattactgt gccaaagtgggtaatttgtatttctttgaatac IGA1 30 213 20 10 8 14 12 16 8 1 10 11 JY8QFUQ01A152R 16874 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 915 90.91 200/220 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [2.17.38.11] CTRDIGGRYSYW in-frame - agcctccggattcacctttagtaattactggatgcactgggtccgccaagctccaggggagggactggtgtgggtctcacggactaatgaagatgggagtatcacaaactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtttctgcaaatgaacagtctcagagtcgaggacacggctgtttattactgtacaagagacatcggtgggcgctactcctactggggccagggagtcctggtcaccgtctcctcagcatccccgaccagcccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccca NA IGA1 100 95 [282, 281] t78>c|c90>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a142>g,K48>E(+ - -)|g147>a|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|a263>t,Y88>F(- + -)|g282>c|c287>t,A96>V(+ - +)|g303>t|g313>a,A105>T(- - -)| t78>c| c90>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a142>g,K48>E(+ - -)|g147>a|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|a263>t,Y88>F(- + -)|g282>c|c287>t,A96>V(+ - +)|g303>t| g313>a,A105>T(- - -)| 320 228 207 21 6 15 3 6 3 2 0 0 1 1 1 1 2 1 78 7 6 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 2 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 1 1 0 0 0 0 0 0 0 0 0 1 30 24 19 5 0 5 1 1 0 1 0 0 0 1 0 0 1 0 117 114 106 8 2 6 1 2 1 0 0 0 1 0 1 1 1 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctcc ggattcacctttagtaattactgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtttctgcaaatgaacagtctcagagtcgaggacacggctgtttattactgt acaagagacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttagtaattactgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtttctgcaaatgaacagtctcagagtcgaggacacggctgtttattactgt acaagagacatcggtgggcgctactcctac IGA1 19 213 12 7 8 12 4 7 4 1 7 7 JY8QFUQ01A15L6 12941 productive (see comment) Homsap IGHV4-61*02 F 816 85.91 189/220 nt 85 187/220 nt Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F 201 88.24 45/51 nt Homsap IGHD3-10*01 F 2 10 7 18 10.7.18 [1.17.37.11] CAREDYYGSGSYGRAWFDSW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 63 of V-REGION: 'C' (1 nucleotides at position 99 to 99 in the user submitted sequence), (cause frameshift); in FR3-IMGT, from codon 68 of V-REGION: 1 nucleotides (from position 116 in the user submitted sequence), (cause frameshift) tctctggaggctccatcagcagtggaagttactactggacctggatccggcagcccgccgggaagactctggagtggattgggcgcttctacagtcgtCgggggtgtcgactacaaccctccctcaggggtcgagtcaccatttcagcggacacgtccaagagccagttctcccttaatctgacttctgcgaccgccactgacacggccgtgtatttctgtgcgagagaggattactatggttcggggagctatgggcgggcctggttcgactcctggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [306] t81>a|t105>a|g119>c,S40>T(+ - +)|g145>a,G49>T(+ - -)|g146>c,G49>T(+ - -)|a147>t,G49>T(+ - -)|t165>c|a166>t,I56>F(+ - -)|t171>c|c173>g,T58>S(+ - +)|c174>t,T58>S(+ - +)|a175>c,S59>R(- - -)|a190>g,S64>G(+ + -)|c192>t,S64>G(+ + -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|a196>g,N66>D(+ + -)|a215>g,K72>R(+ + +)|a220>g,S74>G(+ + -)|a234>t|t239>c,V80>A(+ - +)|a240>g,V80>A(+ - +)|a254>g,N85>S(- - -)|g267>t|g270>t,K90>N(+ - -)|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|t281>c,V94>A(+ - +)|g289>a,A97>T(- - -)|a291>t,A97>T(- - -)|a308>t,Y103>F(- + -)| t81>a|t105>a| g119>c,S40>T(+ - +)|g145>a,G49>T(+ - -)|g146>c,G49>T(+ - -)|a147>t,G49>T(+ - -)|t165>c| a166>t,I56>F(+ - -)|t171>c|c173>g,T58>S(+ - +)|c174>t,T58>S(+ - +)|a175>c,S59>R(- - -)|a190>g,S64>G(+ + -)|c192>t,S64>G(+ + -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)| a196>g,N66>D(+ + -)|a215>g,K72>R(+ + +)|a220>g,S74>G(+ + -)|a234>t|t239>c,V80>A(+ - +)|a240>g,V80>A(+ - +)|a254>g,N85>S(- - -)|g267>t|g270>t,K90>N(+ - -)|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|t281>c,V94>A(+ - +)|g289>a,A97>T(- - -)|a291>t,A97>T(- - -)|a308>t,Y103>F(- + -)| 320 228 197 31 6 25 7 2 4 4 1 0 5 2 3 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 2 0 0 0 0 0 0 0 0 2 0 0 0 0 51 51 46 5 1 4 0 1 0 1 0 0 1 0 2 0 0 0 30 21 12 9 1 8 2 0 3 1 1 0 1 0 0 1 0 0 117 113 98 15 2 13 5 1 1 2 0 0 3 0 1 0 2 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggaggctccatcagcagtggaagttactac tggacctggatccggcagcccgccgggaagactctggagtggattgggcgc ttctacagtcgtgggggtgtc gactacaaccctccctcaggggtcgagtcaccatttcagcggacacgtccaagagccagttctcccttaatctgacttctgcgaccgccactgacacggccgtgtatttctgt gcgagagaggattactatggttcggggagctatgggcgggcctggttcgactcc AREDYYGSGSYGRAWFDS IGHV4-61 IGHD3-10 IGHJ5 ggaggctccatcagcagtggaagttactac tggacctggatccggcagcccgccgggaagactctggagtggattgggcgc ttctacagtcgtgggggtgtc gactacaaccctccctcaggggtcgagtcaccatttcagcggacacgtccaagagccagttctcccttaatctgacttctgcgaccgccactgacacggccgtgtatttctgt gcgagagaggattactatggttcggggagctatgggcgggcctggttcgactcc IGA1 31 215 17 14 6 12 11 19 3 3 17 8 JY8QFUQ01A15SR 6662 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F (see comment) 643 77.78 168/216 nt NA Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD3-10*02 F 2 10 7 11 10.7.11 [X.17.38.11] CVRHVRRFWFDPW in-frame - Low V-REGION identity (77,78% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release cctctggtttcacggttcagtggctctgctatgcactgggtccgccaggcttccgggaagggtctggagtacattggaagcatcttttatagtgggagcacttacttcaatccgtccctcaagagtcgagtcaccctatccgtagacacgtccaggaaccagttctccctgaggctgaagtctgtgaccgccgcagacacggctgtttattattgtgtgagacatgttcgacgattctggttcgacccctggggccagggaaccctggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [280] g79>c,G27>P(+ - -)|g80>c,G27>P(+ - -)|g82>c,G28>L(- - -)|g83>t,G28>L(- - -)|c84>g,G28>L(- - -)|t85>g,S29>V(- - -)|c86>t,S29>V(- - -)|c87>t,S29>V(- - -)|a88>t,I30>S(- - -)|t89>c,I30>S(- - -)|c90>a,I30>S(- - -)|a91>c,S31>R(- - -)|c93>g,S31>R(- - -)|a100>t,S34>F(- - -)|g101>t,S34>F(- - -)|t102>c,S34>F(- - -)|a106>g,S36>G(+ + -)|t108>c,S36>G(+ + -)|a110>c,Y37>S(+ - -)|c111>t,Y37>S(+ - -)|t112>g,Y38>A(- - -)|a113>c,Y38>A(- - -)|c114>t,Y38>A(- - -)|t115>a,W39>M(+ - -)|g116>t,W39>M(+ - -)|g118>c,G40>H(+ - -)|g119>a,G40>H(+ - -)|a124>g,I42>V(+ - +)|c133>g,P45>A(- - -)|c135>t,P45>A(- - -)|c136>t,P46>S(+ - -)|a138>c,P46>S(+ - -)|g147>t|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|g162>a|t165>c|a170>t,Y57>F(- + -)|c195>t|a200>t,Y67>F(- + -)|c204>t|a232>c,I78>L(+ + +)|a251>g,K84>R(+ + +)|a269>g,K90>R(+ + +)|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g303>t|c309>t|c314>t,A105>V(+ - +)| g79>c,G27>P(+ - -)|g80>c,G27>P(+ - -)|g82>c,G28>L(- - -)|g83>t,G28>L(- - -)|c84>g,G28>L(- - -)|t85>g,S29>V(- - -)|c86>t,S29>V(- - -)|c87>t,S29>V(- - -)|a88>t,I30>S(- - -)|t89>c,I30>S(- - -)|c90>a,I30>S(- - -)|a91>c,S31>R(- - -)|c93>g,S31>R(- - -)|a100>t,S34>F(- - -)|g101>t,S34>F(- - -)|t102>c,S34>F(- - -)|a106>g,S36>G(+ + -)|t108>c,S36>G(+ + -)|a110>c,Y37>S(+ - -)|c111>t,Y37>S(+ - -)|t112>g,Y38>A(- - -)|a113>c,Y38>A(- - -)|c114>t,Y38>A(- - -)| t115>a,W39>M(+ - -)|g116>t,W39>M(+ - -)|g118>c,G40>H(+ - -)|g119>a,G40>H(+ - -)|a124>g,I42>V(+ - +)|c133>g,P45>A(- - -)|c135>t,P45>A(- - -)|c136>t,P46>S(+ - -)|a138>c,P46>S(+ - -)|g147>t|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|g162>a|t165>c| a170>t,Y57>F(- + -)|c195>t| a200>t,Y67>F(- + -)|c204>t|a232>c,I78>L(+ + +)|a251>g,K84>R(+ + +)|a269>g,K90>R(+ + +)|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g303>t|c309>t| c314>t,A105>V(+ - +)| 320 224 175 49 7 42 4 4 10 4 5 1 4 1 5 4 5 2 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 7 23 0 23 1 0 4 3 3 1 2 0 3 2 2 2 51 51 37 14 3 11 1 3 2 1 1 0 0 1 2 1 2 0 30 21 19 2 1 1 0 0 1 0 0 0 1 0 0 0 0 0 117 114 105 9 3 6 2 1 2 0 1 0 1 0 0 1 1 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| cctctggtttcacggttcagtggctctgct atgcactgggtccgccaggcttccgggaagggtctggagtacattggaagc atcttttatagtgggagcact tacttcaatccgtccctcaagagtcgagtcaccctatccgtagacacgtccaggaaccagttctccctgaggctgaagtctgtgaccgccgcagacacggctgtttattattgt gtgagacatgttcgacgattctggttcgacccc VRHVRRFWFDP IGHV4-39 IGHD3-10 IGHJ5 cctctggtttcacggttcagtggctctgct atgcactgggtccgccaggcttccgggaagggtctggagtacattggaagc atcttttatagtgggagcact tacttcaatccgtccctcaagagtcgagtcaccctatccgtagacacgtccaggaaccagttctccctgaggctgaagtctgtgaccgccgcagacacggctgtttattattgt gtgagacatgttcgacgattctggttcgacccc IGA1 48 216 21 27 13 28 8 20 6 1 17 24 JY8QFUQ01A16XV 9554 productive Homsap IGHV3-48*01 F 1080 100 217/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-2*01 F 2 8 8 10 8.8.10 [1.17.38.11] CASRLRSSGGYW in-frame + ctctggattcaccttcagtagctatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagtagtagtagtagtaccatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagccgcctacgtagttccggtgggtactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 86 94 [279] a318>c,R106>S(- - -)|g319>c|a320>g| 317 (320) 222 (225) 222 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtagtagtagtagtaccata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagccgcctacgtagttccggtgggtac ASRLRSSGGY IGHV3-48 IGHD2-2 IGHJ4 ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtagtagtagtagtaccata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagccgcctacgtagttccggtgggtac IGG1 0 213 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01A17D9 15740 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 912 92.06 197/214 nt NA Homsap IGHJ6*02 F 202 80.65 50/62 nt Homsap IGHD3-16*01 F 2 8 8 14 8.8.14 [0.17.38.11] CARHNYGNEKYIMDVW in-frame - tggatacaactttgccacctattggatcggctgggtgcgccacatgcccgggaaaggcctggaatggatggggatggtctttgctggtgactctgacaccagatacagtccgtccttccgaggccaggtcaccatgtcagccgacaagtccatcaacaccgcctacctgcagtggagcagcctgatggcctcggacaccgccatatattactgtgcgagacataattatggaaacgagaagtacattatggacgtctggggccaggggaccacggtcaccgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] g86>a,S29>N(- - -)|a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)|c111>t|g132>c,Q44>H(- + -)|g153>a|c165>g,I55>M(+ + -)|a166>g,I56>V(+ - +)|a170>t,Y57>F(- + -)|c172>g,P58>A(- - -)|t192>c|c204>t|a215>g,Q72>R(+ - -)|c234>g,I78>M(+ + -)|g254>a,S85>N(- - -)|a284>t,K95>M(- + -)|g303>a,M101>I(+ + -)| g86>a,S29>N(- - -)|a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)|c111>t| g132>c,Q44>H(- + -)|g153>a|c165>g,I55>M(+ + -)| a166>g,I56>V(+ - +)|a170>t,Y57>F(- + -)|c172>g,P58>A(- - -)|t192>c| c204>t|a215>g,Q72>R(+ - -)|c234>g,I78>M(+ + -)|g254>a,S85>N(- - -)|a284>t,K95>M(- + -)|g303>a,M101>I(+ + -)| 320 222 205 17 4 13 3 4 2 1 0 0 2 0 2 3 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 1 0 0 0 0 0 1 0 0 0 51 51 48 3 1 2 0 1 0 0 0 0 0 0 1 1 0 0 30 24 20 4 1 3 1 0 0 1 0 0 1 0 0 1 0 0 117 114 108 6 1 5 1 2 1 0 0 0 1 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaactttgccacctattgg atcggctgggtgcgccacatgcccgggaaaggcctggaatggatggggatg gtctttgctggtgactctgacacc agatacagtccgtccttccgaggccaggtcaccatgtcagccgacaagtccatcaacaccgcctacctgcagtggagcagcctgatggcctcggacaccgccatatattactgt gcgagacataattatggaaacgagaagtacattatggacgtc ARHNYGNEKYIMDV IGHV5-51 IGHD3-16 IGHJ6 ggatacaactttgccacctattgg atcggctgggtgcgccacatgcccgggaaaggcctggaatggatggggatg gtctttgctggtgactctgacacc agatacagtccgtccttccgaggccaggtcaccatgtcagccgacaagtccatcaacaccgcctacctgcagtggagcagcctgatggcctcggacaccgccatatattactgt gcgagacataattatggaaacgagaagtacattatggacgtc IGA1 17 213 10 7 6 11 4 6 2 2 7 6 JY8QFUQ01A17TV 13332 productive (see comment) Homsap IGHV3-11*01 F 934 93.43 199/213 nt 92.02 196/213 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-15*01 F 2 8 7 18 8.7.18 [1.17.38.11] CARDGPPYLYGSHGNSFDQW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 40 of V-REGION: 'T' (1 nucleotides at position 33 to 33 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 52 of V-REGION: 'C' (1 nucleotides at position 69 to 69 in the user submitted sequence), (cause frameshift); in CDR2-IMGT, from codon 63 of V-REGION: 3 nucleotides (from position 94 in the user submitted sequence), (do not cause frameshift) tctggattcagcttcagtgactactacatgagTttgggtccgccaggctccagggaagggactggagtCgggtttcatgcatcactactagtggtaccacattctacacggactctgtgaggggccgattcaccatgtccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtgtattactgtgcgagagatggcccaccttatttatatggaagtcatggtaattcctttgaccagtggggccagggaaccctagtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [301] c86>g,T29>S(+ - +)|c120>t|a124>g,I42>V(+ - +)|g147>a|a164>g,Y55>C(- - -)|t168>c|g170>c,S57>T(+ - +)|g173>c,S58>T(+ - +)|t194>c,I65>T(- - -)|a197>t,Y66>F(- + -)|g202>a,A68>T(- - -)|a204>g,A68>T(- - -)|a215>g,K72>R(+ + +)|c234>g,I78>M(+ + -)| c86>g,T29>S(+ - +)| c120>t|a124>g,I42>V(+ - +)|g147>a|a164>g,Y55>C(- - -)| t168>c|g170>c,S57>T(+ - +)|g173>c,S58>T(+ - +)|t194>c,I65>T(- - -)| a197>t,Y66>F(- + -)|g202>a,A68>T(- - -)|a204>g,A68>T(- - -)|a215>g,K72>R(+ + +)|c234>g,I78>M(+ + -)| 320 221 207 14 3 11 4 2 1 2 0 0 1 0 2 2 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 47 4 2 2 2 1 1 0 0 0 0 0 0 0 0 0 30 21 17 4 1 3 0 0 0 2 0 0 0 0 2 0 0 0 117 114 109 5 0 5 2 1 0 0 0 0 1 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcagcttcagtgactactac atgagttgggtccgccaggctccagggaagggactggagtgggtttcatgc atcactactagtggtaccaca ttctacacggactctgtgaggggccgattcaccatgtccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtgtattactgt gcgagagatggcccaccttatttatatggaagtcatggtaattcctttgaccag ARDGPPYLYGSHGNSFDQ IGHV3-11 IGHD2-15 IGHJ4 ggattcagcttcagtgactactac atgagttgggtccgccaggctccagggaagggactggagtgggtttcatgc atcactactagtggtaccaca ttctacacggactctgtgaggggccgattcaccatgtccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtgtattactgt gcgagagatggcccaccttatttatatggaagtcatggtaattcctttgaccag IGG2 14 210 9 5 3 7 6 7 2 1 7 4 JY8QFUQ01A18L5 17894 productive Homsap IGHV1-18*01 F 957 94.39 202/214 nt NA Homsap IGHJ6*02 F 247 88.71 55/62 nt Homsap IGHD3-3*01 F 1 8 8 20 8.8.20 [0.17.38.11] CARDHRSGALEWSHHYHGMDVW in-frame + tggttacatcttcacccactatggtatcaactgggtgcgccaggcccctggacaagggcttgagtggatgggatggatcagcgcctacagtggtaacacaaagtatgcacagaaggtccagggcagagtcaccatgaccacagacacttccacgagtacagcctacatggagctgaggagcctgagatctgacgacacggccgtgtattactgtgcgagagatcacagaagcggggctttggagtggtctcaccactaccacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatcccgaccagcccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 89 [303] c86>t,T29>I(- - -)|t90>c|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|g119>a,S40>N(- - -)|a129>c|t174>c|a185>g,N62>S(- - -)|c198>g,N66>K(+ - -)|c211>g,L71>V(+ - +)|a246>t|c255>t| c86>t,T29>I(- - -)|t90>c|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)| g119>a,S40>N(- - -)|a129>c| t174>c|a185>g,N62>S(- - -)| c198>g,N66>K(+ - -)|c211>g,L71>V(+ - +)|a246>t|c255>t| 320 222 210 12 5 7 1 2 2 2 2 0 1 0 0 2 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 1 1 1 0 0 0 0 0 0 0 51 51 49 2 1 1 0 1 0 0 1 0 0 0 0 0 0 0 30 24 22 2 1 1 1 0 0 1 0 0 0 0 0 0 0 0 117 114 110 4 2 2 0 0 1 0 0 0 1 0 0 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|ta,175-176(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,5-6(FR1)|tt,75-76(FR1)|tt,81-82(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|tt,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,3-6(FR1)|agct,8-11(FR1)|agct,24-27(FR1)|ggct,72-75(FR1)|ggtt,79-82(CDR1)|agct,106-109(CDR1)|ggta,112-115(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)| agct,8-11(FR1)|agct,24-27(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|agct,209-212(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggttacatcttcacccactatggt atcaactgggtgcgccaggcccctggacaagggcttgagtggatgggatgg atcagcgcctacagtggtaacaca aagtatgcacagaaggtccagggcagagtcaccatgaccacagacacttccacgagtacagcctacatggagctgaggagcctgagatctgacgacacggccgtgtattactgt gcgagagatcacagaagcggggctttggagtggtctcaccactaccacggtatggacgtc ARDHRSGALEWSHHYHGMDV IGHV1-18 IGHD3-3 IGHJ6 ggttacatcttcacccactatggt atcaactgggtgcgccaggcccctggacaagggcttgagtggatgggatgg atcagcgcctacagtggtaacaca aagtatgcacagaaggtccagggcagagtcaccatgaccacagacacttccacgagtacagcctacatggagctgaggagcctgagatctgacgacacggccgtgtattactgt gcgagagatcacagaagcggggctttggagtggtctcaccactaccacggtatggacgtc IGA1 12 213 7 5 4 6 3 6 3 2 3 4 JY8QFUQ01A1963 12899 productive (see comment) Homsap IGHV3-64*02 F 962 94.42 203/215 nt 93.95 202/215 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 2 7 8 10 7.8.10 [1.17.38.11] CVTCGDYSYEYW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 29 of V-REGION: 1 nucleotides (from position 11 in the user submitted sequence), (cause frameshift) tctggattcatttcagtacttatcctatgcactgggtccgccaggctccagggaagggactggaatatgtttcagctattagtcgtaatggggataacgcatattatgcagactctgtgaagggcagattcaccatgtccagagacaattccaagagcacactgtatcttcagatgggcagcctgagagctgaggacatggctgtgtattactgtgtgacatgtggtgattacagctatgagtactggggccagggaaccttggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatggaacgtggtcgtcgcatgcctggtccagggcttcttcccca NA IGA2 100 89 [276, 275] c87>t|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>c,A38>P(- - -)|a172>c,S58>R(- - -)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|a193>g,T65>A(- - -)|c234>g,I78>M(+ + -)|a254>g,N85>S(- - -)|g258>a|a270>g|c314>t,A105>V(+ - +)| c87>t|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>c,A38>P(- - -)| a172>c,S58>R(- - -)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|a193>g,T65>A(- - -)| c234>g,I78>M(+ + -)|a254>g,N85>S(- - -)|g258>a|a270>g| c314>t,A105>V(+ - +)|g317>c,R106>T(- - -)|g319>t|a320>g| 316 (320) 219 (223) 206 (207) 13 (16) 3 (5) 10 (11) 3 (4) 3 3 0 1 0 0 0 2 (3) 1 0 (1) 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 19 4 1 3 0 0 2 0 0 0 0 0 2 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 2 0 0 1 0 0 0 0 0 0 0 117 114 110 4 2 2 2 1 0 0 0 0 0 0 0 1 0 0 4 (8) 4 3 (4) 1 (4) 0 (2) 1 (2) 0 (1) 0 1 0 0 0 0 0 0 (1) 0 0 (1) 0 aa,26-27(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|aa,152-153(FR2)|ta,154-155(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,154-155(FR2)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|tt,198-199(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|agct,162-165(FR2)|agta,169-172(CDR2)|ggta,187-190(CDR2)|ggca,220-223(FR3)|ggca,274-277(FR3)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|agct,162-165(FR2)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| tct ggattcatttcagtacttatcct atgcactgggtccgccaggctccagggaagggactggaatatgtttcagct attagtcgtaatggggataacgca tattatgcagactctgtgaagggcagattcaccatgtccagagacaattccaagagcacactgtatcttcagatgggcagcctgagagctgaggacatggctgtgtattactgt gtgacatgtggtgattacagctatgagtac VTCGDYSYEY IGHV3-64 IGHD3-22 IGHJ4 ggattcatttcagtacttatcct atgcactgggtccgccaggctccagggaagggactggaatatgtttcagct attagtcgtaatggggataacgca tattatgcagactctgtgaagggcagattcaccatgtccagagacaattccaagagcacactgtatcttcagatgggcagcctgagagctgaggacatggctgtgtattactgt gtgacatgtggtgattacagctatgagtac IGA2 12 212 8 4 5 8 3 4 2 1 2 7 JY8QFUQ01A1ALH 15694 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 902 90.05 199/221 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 2 10 7 12 10.7.12 [1.17.38.11] CARHSRLVRYFEFW in-frame + tctctggtggctccatcaacagtggtagttatcactgggcctggatccgccagtccccagggaaggggctggagtggattgggagcgtctcctatggtgggaacacctactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgtgcgagacattcgagactggtacggtactttgaattctggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [288] g92>a,S31>N(- - -)|a103>g,S35>G(+ + -)|c111>t|t112>c,Y38>H(+ - -)|g119>c,G40>A(- + -)|c133>t,P45>S(+ - -)|t165>c|a166>g,I56>V(+ - +)|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a175>g,S59>G(+ + -)|g191>a,S64>N(- - -)|a215>t,K72>M(- + -)|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|c236>t,S79>F(- - -)|a240>c|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c261>g,F87>L(+ - -)|c271>g,L91>V(+ - +)|a291>g| g92>a,S31>N(- - -)|a103>g,S35>G(+ + -)|c111>t|t112>c,Y38>H(+ - -)| g119>c,G40>A(- + -)|c133>t,P45>S(+ - -)|t165>c| a166>g,I56>V(+ - +)|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a175>g,S59>G(+ + -)|g191>a,S64>N(- - -)| a215>t,K72>M(- + -)|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|c236>t,S79>F(- - -)|a240>c|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c261>g,F87>L(+ - -)|c271>g,L91>V(+ - +)|a291>g| 320 229 207 22 4 18 6 2 4 3 2 1 1 0 1 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 1 3 1 1 1 1 0 0 0 0 0 0 0 0 51 51 48 3 1 2 0 0 1 1 0 0 0 0 1 0 0 0 30 21 16 5 0 5 2 1 0 1 1 0 0 0 0 0 0 0 117 114 104 10 2 8 3 0 2 0 1 1 1 0 0 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcaacagtggtagttatcac tgggcctggatccgccagtccccagggaaggggctggagtggattgggagc gtctcctatggtgggaacacc tactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgt gcgagacattcgagactggtacggtactttgaattc ARHSRLVRYFEF IGHV4-39 IGHD5-18 IGHJ4 ggtggctccatcaacagtggtagttatcac tgggcctggatccgccagtccccagggaaggggctggagtggattgggagc gtctcctatggtgggaacacc tactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgt gcgagacattcgagactggtacggtactttgaattc IGA1 22 216 15 7 6 10 9 12 3 1 10 8 JY8QFUQ01A1AYP 20529 productive Homsap IGHV3-7*01 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD5-18*01 F 3 8 8 15 8.8.15 [1.17.38.11] CARVGYGYRGIYFFDYW in-frame + ctctacattcacgtttagtcggtattggatgagctgggtccgccaggctccagggaagggcctggagtgggtggccaacataaaggaagacggaagtgagagatattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgtgcgagagtgggctatggttaccgaggaatatacttctttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG2 100 100 [294] g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|c87>g|a106>c,S36>R(- - -)|c108>g,S36>R(- - -)|g147>c|c172>g,Q58>E(+ + -)|t177>c|a194>g,K65>R(+ + +)|c198>t|c246>t|a258>t|a271>t,M91>L(+ + -)|c280>t| g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|c87>g|a106>c,S36>R(- - -)|c108>g,S36>R(- - -)| g147>c| c172>g,Q58>E(+ + -)|t177>c|a194>g,K65>R(+ + +)| c198>t|c246>t|a258>t|a271>t,M91>L(+ + -)|c280>t| a320>t| 319 (320) 224 (225) 210 14 (15) 7 (8) 7 1 1 3 1 1 0 2 (3) 0 2 3 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 0 0 1 0 0 0 1 2 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 21 3 1 2 1 0 0 1 0 0 0 0 0 1 0 0 117 114 109 5 4 1 0 0 3 0 0 0 2 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct acattcacgtttagtcggtattgg atgagctgggtccgccaggctccagggaagggcctggagtgggtggccaac ataaaggaagacggaagtgagaga tattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgt gcgagagtgggctatggttaccgaggaatatacttctttgactac ARVGYGYRGIYFFDY IGHV3-7 IGHD5-18 IGHJ4 acattcacgtttagtcggtattgg atgagctgggtccgccaggctccagggaagggcctggagtgggtggccaac ataaaggaagacggaagtgagaga tattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgt gcgagagtgggctatggttaccgaggaatatacttctttgactac IGG2 14 213 6 8 4 9 2 5 5 2 1 6 JY8QFUQ01A1BK7 5600 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 945 93.09 202/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [1.17.38.11] CTRDIGGRYSYW in-frame + ctccggattcacctttagtagttactggatgcactgggtccgccaagctccaggggagggactggtgtgggtctcacggactaatgaagatgggagtatcacaaactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgtacaagagacatcggtgggcgctactcctactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcaccccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccaa NA IGA1 79 95 [278, 279] t78>c|c90>t|c108>t|a142>g,K48>E(+ - -)|g147>a|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|g313>a,A105>T(- - -)| t78>c| c90>t|c108>t| a142>g,K48>E(+ - -)|g147>a|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)| g313>a,A105>T(- - -)| 320 225 209 16 5 11 3 5 2 2 0 0 0 1 0 1 1 1 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 22 2 2 0 0 0 2 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 1 1 0 0 0 0 0 0 0 0 0 1 30 24 19 5 0 5 1 1 0 1 0 0 0 1 0 0 1 0 117 114 110 4 0 4 1 2 0 0 0 0 0 0 0 1 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcacctttagtagttactgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt acaagagacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttagtagttactgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt acaagagacatcggtgggcgctactcctac IGA1 14 213 10 4 6 8 4 6 2 2 5 5 JY8QFUQ01A1BT3 12909 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 854 88.84 191/215 nt 88.37 190/215 nt Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD3-16*02 F 2 8 7 19 8.7.19 [1.17.38.11] CARSSDYLWGSYRYPYYFDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 42 of V-REGION: 'G' (1 nucleotides at position 41 to 41 in the user submitted sequence), (cause frameshift); tctctggtgactccatcagtagttacttctggagttggatGccggcagcccccagggaagggactggagtggattggatatgtctattacagtggaagtaccaagtataatccttccctcgaaagtcgagtcaccatatcattagacacgcccaacaaccagttctccctgagcctgacctatgtcaccgctgcggacacggccatatactactgtgcgagaagctctgattacctttgggggagttatcgttatccctactacttcgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [304] g83>a,G28>D(- - -)|a113>t,Y38>F(- + -)|c120>t|g162>a|a166>g,I56>V(+ - +)|g189>a|c192>t|c198>g,N66>K(+ - -)|c201>t|c204>t|c207>t|a214>g,K72>E(+ - -)|g216>a,K72>E(+ - -)|g238>t,V80>L(+ - +)|t247>c,S83>P(+ - -)|g252>c,K84>N(+ - -)|a269>g,K90>S(- - -)|g270>c,K90>S(- - -)|g275>c,S92>T(+ - +)|c278>a,S93>Y(+ - -)|g282>c|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|t306>c| g83>a,G28>D(- - -)|a113>t,Y38>F(- + -)| c120>t|g162>a| a166>g,I56>V(+ - +)|g189>a|c192>t| c198>g,N66>K(+ - -)|c201>t|c204>t|c207>t|a214>g,K72>E(+ - -)|g216>a,K72>E(+ - -)|g238>t,V80>L(+ - +)|t247>c,S83>P(+ - -)|g252>c,K84>N(+ - -)|a269>g,K90>S(- - -)|g270>c,K90>S(- - -)|g275>c,S92>T(+ - +)|c278>a,S93>Y(+ - -)|g282>c|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|t306>c| g319>a|a320>g| 318 (320) 221 (223) 197 24 (26) 9 (11) 15 3 (4) 6 (7) 5 2 0 1 1 0 4 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 1 0 0 0 0 0 51 51 49 2 2 0 0 1 1 0 0 0 0 0 0 0 0 0 30 21 18 3 2 1 1 1 1 0 0 0 0 0 0 0 0 0 117 114 97 17 5 12 2 3 3 2 0 1 0 0 4 1 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatcagtagttacttc tggagttggatccggcagcccccagggaagggactggagtggattggatat gtctattacagtggaagtacc aagtataatccttccctcgaaagtcgagtcaccatatcattagacacgcccaacaaccagttctccctgagcctgacctatgtcaccgctgcggacacggccatatactactgt gcgagaagctctgattacctttgggggagttatcgttatccctactacttcgactac ARSSDYLWGSYRYPYYFDY IGHV4-59 IGHD3-16 IGHJ4 ggtgactccatcagtagttacttc tggagttggatccggcagcccccagggaagggactggagtggattggatat gtctattacagtggaagtacc aagtataatccttccctcgaaagtcgagtcaccatatcattagacacgcccaacaaccagttctccctgagcctgacctatgtcaccgctgcggacacggccatatactactgt gcgagaagctctgattacctttgggggagttatcgttatccctactacttcgactac IGA2 24 210 16 8 11 18 5 6 7 2 12 3 JY8QFUQ01A1CLZ 5944 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 933 91.82 202/220 nt 91.36 201/220 nt Homsap IGHJ5*02 F 183 84.31 43/51 nt Homsap IGHD3-10*01 F 2 10 7 16 10.7.16 [1.17.38.11] CVRPDNYFGSGSAWMDPW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 71 of V-REGION: 'CA' (2 nucleotides at position 123 to 124 in the user submitted sequence), (cause frameshift); ctctggtggctccatcagcagtgataatttctactggggctggatccgccagcccccagggaagggactgcagtggattgggactttctattatagagggagtatctattacaacccgtcccCAtcaagagtcgagtcaccatatccgtggacacatccaagaaccagttctccctgaggctgacctctgtgaccgccgcagacacggctgtctattattgtgtgagacccgataattactttggttcggggagtgcctggatggacccctggggccacggaaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [302] a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)|g147>a|g151>c,E51>Q(+ + -)|g164>c,S55>T(+ - +)|a166>t,I56>F(+ - -)|t177>a,S59>R(- - -)|c192>t|c194>t,T65>I(- - -)|c198>t|a240>g|g246>a|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|g303>c|c309>t|c314>t,A105>V(+ - +)| a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)| g147>a|g151>c,E51>Q(+ + -)|g164>c,S55>T(+ - +)| a166>t,I56>F(+ - -)|t177>a,S59>R(- - -)|c192>t|c194>t,T65>I(- - -)| c198>t|a240>g|g246>a|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|g303>c|c309>t| c314>t,A105>V(+ - +)|a320>c| 319 (320) 227 (228) 208 19 (20) 7 (8) 12 3 4 5 0 0 (1) 0 2 1 4 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 0 4 1 2 0 0 0 0 1 0 0 0 0 0 51 51 48 3 1 2 0 1 0 0 0 0 0 0 2 0 0 0 30 21 17 4 1 3 0 0 2 0 0 0 1 1 0 0 0 0 117 114 107 7 5 2 2 1 2 0 0 0 0 0 2 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtggctccatcagcagtgataatttctac tggggctggatccgccagcccccagggaagggactgcagtggattgggact ttctattatagagggagtatc tattacaacccgtccctcaagagtcgagtcaccatatccgtggacacatccaagaaccagttctccctgaggctgacctctgtgaccgccgcagacacggctgtctattattgt gtgagacccgataattactttggttcggggagtgcctggatggacccc VRPDNYFGSGSAWMDP IGHV4-39 IGHD3-10 IGHJ5 ggtggctccatcagcagtgataatttctac tggggctggatccgccagcccccagggaagggactgcagtggattgggact ttctattatagagggagtatc tattacaacccgtccctcaagagtcgagtcaccatatccgtggacacatccaagaaccagttctccctgaggctgacctctgtgaccgccgcagacacggctgtctattattgt gtgagacccgataattactttggttcggggagtgcctggatggacccc IGG1 18 216 11 7 8 12 3 6 6 1 4 7 JY8QFUQ01A1CTT 20836 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 945 93.09 202/217 nt 91.71 199/217 nt Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD3-16*01 F 2 8 8 16 8.8.16 [1.17.38.11] CARRLNNYDSGEYAMDVW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'G' (1 nucleotides at position 8 to 8 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 57 to 57 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 53 of V-REGION: 'CG' (2 nucleotides at position 75 to 76 in the user submitted sequence), (cause frameshift); ttctggaGtacacgtttatgaattactggatcggctgggtgcgccagatgcccgggAaaaggcctggagtggatCGggggatcatctatcctggtacctctgagaccagatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgcctacgtgcagtggagcagcctgaaggccccggacaccgccatatattactgtgcgaggcgactcaataattacgactcgggagagtacgctatggacgtctggggccaagggaccacggtcaccgtctcctcaacctccaccaagggcccatcggtcttcccccctggcaccctcctccaagagcacctctggggggcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [302, 301] g86>c,S29>T(+ - +)|c87>g,S29>T(+ - +)|c104>t,T35>M(- - -)|c105>g,T35>M(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g184>a,D62>T(- + -)|a185>c,D62>T(- + -)|t192>g,D64>E(+ - +)|c213>t|c234>t|t251>c,I84>T(- - -)|c265>g,L89>V(+ - +)|t289>c,S97>P(+ - -)|g303>a,M101>I(+ + -)| g86>c,S29>T(+ - +)|c87>g,S29>T(+ - +)|c104>t,T35>M(- - -)|c105>g,T35>M(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g184>a,D62>T(- + -)|a185>c,D62>T(- + -)|t192>g,D64>E(+ - +)| c213>t|c234>t|t251>c,I84>T(- - -)|c265>g,L89>V(+ - +)|t289>c,S97>P(+ - -)|g303>a,M101>I(+ + -)| a318>g|a320>g| 317 (320) 222 (225) 207 (208) 15 (17) 2 (4) 13 0 (2) 3 4 2 1 0 0 0 1 3 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 1 2 0 0 0 0 0 1 2 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 1 0 0 1 0 0 0 0 0 0 1 117 114 108 6 2 4 0 1 2 2 0 0 0 0 0 1 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ttct ggatacacgtttatgaattactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtacctctgagacc agatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgcctacgtgcagtggagcagcctgaaggccccggacaccgccatatattactgt gcgaggcgactcaataattacgactcgggagagtacgctatggacgtc ARRLNNYDSGEYAMDV IGHV5-51 IGHD3-16 IGHJ6 ggatacacgtttatgaattactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtacctctgagacc agatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgcctacgtgcagtggagcagcctgaaggccccggacaccgccatatattactgt gcgaggcgactcaataattacgactcgggagagtacgctatggacgtc IGG1 15 213 9 6 7 11 2 4 2 0 4 9 JY8QFUQ01A1DJR 16533 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 954 93.55 203/217 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD5-12*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARGPRSRGYGMDVW in-frame + ctctggattcaccttcaacaactatgccatgagttgggtccgccaggctccagggaaggggctggaatgggtctcaactattactagtggtggtggtagtacattgtacgcagactccgtgaagggccggttcaccatctccagagacaatttcaaggacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgagagggcctcgatcgcgcggctacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [288] t90>c|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|c120>t|g153>a|g163>a,A55>T(- - -)|g170>c,S57>T(+ - +)|g172>a,G58>S(+ + -)|a175>g,S59>G(+ + -)|c192>t|a197>t,Y66>L(- - -)|c198>g,Y66>L(- - -)|c248>t,S83>F(- - -)|a253>g,N85>D(+ + -)|a317>g,K106>R(+ + +)| t90>c|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)| c120>t|g153>a|g163>a,A55>T(- - -)| g170>c,S57>T(+ - +)|g172>a,G58>S(+ + -)|a175>g,S59>G(+ + -)|c192>t| a197>t,Y66>L(- - -)|c198>g,Y66>L(- - -)|c248>t,S83>F(- - -)|a253>g,N85>D(+ + -)| a317>g,K106>R(+ + +)|a320>g| 319 (320) 224 (225) 209 15 (16) 4 (5) 11 3 (4) 5 3 1 0 0 1 0 1 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 2 0 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 2 1 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 1 1 1 0 0 0 0 0 1 0 0 0 117 114 110 4 0 4 1 0 1 0 0 0 1 0 0 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 1 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcaacaactatgcc atgagttgggtccgccaggctccagggaaggggctggaatgggtctcaact attactagtggtggtggtagtaca ttgtacgcagactccgtgaagggccggttcaccatctccagagacaatttcaaggacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagagggcctcgatcgcgcggctacggtatggacgtc ARGPRSRGYGMDV IGHV3-23 IGHD5-12 IGHJ6 ggattcaccttcaacaactatgcc atgagttgggtccgccaggctccagggaaggggctggaatgggtctcaact attactagtggtggtggtagtaca ttgtacgcagactccgtgaagggccggttcaccatctccagagacaatttcaaggacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagagggcctcgatcgcgcggctacggtatggacgtc IGG1 14 213 11 3 8 10 3 4 2 2 5 5 JY8QFUQ01A1DVA 8304 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F (see comment) 765 83.87 182/217 nt NA Homsap IGHJ6*02 F (see comment) 202 80.65 50/62 nt Homsap IGHD4-11*01 ORF 2 8 8 17 8.8.17 [1.17.38.11] CARDSALFGYARYDAMDAW in-frame - Low V-REGION identity (83,87% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) ctctggatccagtttcagtggttttggcgtgaactgggtccgccaggctccagggaaggggctggaatgggtctcacacgtcaatagtgccagtgactacaaatattacgcggactcagtgaggggccggttcaccatttccagagacaatgccaagaactcagtgtatctgcgaatgaataacctgagagacgacgacacggctctatattactgtgcgagagacagcgccttattcggctacgctaggtacgacgctatggacgcctggggccaagggaccgcggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [300] t83>c,F28>S(- - -)|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)|a110>t,Y37>F(- + -)|a112>g,S38>G(+ + -)|a115>g,M39>V(+ - -)|g153>a|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>a,S57>N(- - -)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|t177>c,S59>A(- + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|t189>c,S63>D(- - -)|t194>a,I65>K(- + -)|c198>t|a204>g|a215>g,K72>R(+ + +)|a225>g|c234>t|c246>t|c259>g,L87>V(+ - +)|a269>g,Q90>R(+ - -)|c276>t|g278>a,S93>N(- - -)|c287>a,A96>D(- - -)|g291>c,E97>D(+ - +)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| t83>c,F28>S(- - -)|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)|a110>t,Y37>F(- + -)|a112>g,S38>G(+ + -)| a115>g,M39>V(+ - -)|g153>a|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>a,S57>N(- - -)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|t177>c,S59>A(- + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|t189>c,S63>D(- - -)|t194>a,I65>K(- + -)| c198>t|a204>g|a215>g,K72>R(+ + +)|a225>g|c234>t|c246>t|c259>g,L87>V(+ - +)|a269>g,Q90>R(+ - -)|c276>t|g278>a,S93>N(- - -)|c287>a,A96>D(- - -)|g291>c,E97>D(+ - +)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| 320 225 190 35 7 28 10 5 6 5 0 2 1 1 3 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 0 7 2 0 2 1 0 0 1 0 0 1 0 0 51 51 47 4 1 3 1 1 0 1 0 1 0 0 0 0 0 0 30 24 14 10 0 10 3 2 0 3 0 0 0 1 1 0 0 0 117 114 100 14 6 8 4 2 4 0 0 1 0 0 2 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatccagtttcagtggttttggc gtgaactgggtccgccaggctccagggaaggggctggaatgggtctcacac gtcaatagtgccagtgactacaaa tattacgcggactcagtgaggggccggttcaccatttccagagacaatgccaagaactcagtgtatctgcgaatgaataacctgagagacgacgacacggctctatattactgt gcgagagacagcgccttattcggctacgctaggtacgacgctatggacgcc ARDSALFGYARYDAMDA IGHV3-21 IGHD4-11 IGHJ6 ggatccagtttcagtggttttggc gtgaactgggtccgccaggctccagggaaggggctggaatgggtctcacac gtcaatagtgccagtgactacaaa tattacgcggactcagtgaggggccggttcaccatttccagagacaatgccaagaactcagtgtatctgcgaatgaataacctgagagacgacgacacggctctatattactgt gcgagagacagcgccttattcggctacgctaggtacgacgctatggacgcc IGG2 35 213 26 9 11 18 15 17 7 0 11 17 JY8QFUQ01A1E6T 14365 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 944 93.49 201/215 nt NA Homsap IGHJ2*01 F 220 90.57 48/53 nt Homsap IGHD1-26*01 F 3 8 8 12 8.8.12 [0.17.38.11] CARPFSGRWCFDLW in-frame - ctggattcaccttcagttactcctggatgcactgggtccgccaagttccaggaaaggggccggtgtgggtctcacaaattaaaagtgatgggagtaccccaagttacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagtcgaggacacggctgtttattactgtgcaagaccgtttagtgggaggtggtgcttcgatctctggggccgtggcaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [282] a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a110>c,Y37>S(+ - -)|c134>t,A45>V(+ - +)|g141>a|t149>c,L50>P(- - -)|g164>a,R55>Q(+ - -)|t165>a,R55>Q(+ - -)|t171>a,N57>K(+ - -)|g191>c,S64>T(+ - +)|a193>c,T65>P(+ + -)|c198>t|c287>t,A96>V(+ - +)|g303>t| a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a110>c,Y37>S(+ - -)| c134>t,A45>V(+ - +)|g141>a|t149>c,L50>P(- - -)|g164>a,R55>Q(+ - -)|t165>a,R55>Q(+ - -)| t171>a,N57>K(+ - -)|g191>c,S64>T(+ - +)|a193>c,T65>P(+ + -)| c198>t|c287>t,A96>V(+ - +)|g303>t| g319>c|a320>c| 318 (320) 221 (223) 207 14 (16) 3 (5) 11 0 3 3 1 2 (3) 0 1 2 1 (2) 0 1 0 78 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 1 0 1 0 0 0 0 0 51 51 46 5 1 4 0 2 1 1 0 0 0 1 0 0 0 0 30 24 21 3 0 3 0 0 0 0 1 0 0 1 1 0 0 0 117 114 111 3 2 1 0 0 2 0 0 0 0 0 0 0 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ct ggattcaccttcagttactcctgg atgcactgggtccgccaagttccaggaaaggggccggtgtgggtctcacaa attaaaagtgatgggagtacccca agttacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagtcgaggacacggctgtttattactgt gcaagaccgtttagtgggaggtggtgcttcgatctc ARPFSGRWCFDL IGHV3-74 IGHD1-26 IGHJ2 ggattcaccttcagttactcctgg atgcactgggtccgccaagttccaggaaaggggccggtgtgggtctcacaa attaaaagtgatgggagtacccca agttacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagtcgaggacacggctgtttattactgt gcaagaccgtttagtgggaggtggtgcttcgatctc IGA2 14 213 7 7 6 8 1 6 3 0 5 6 JY8QFUQ01A1GYW 6118 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F (see comment) 696 80.84 173/214 nt NA Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD6-6*01 F 3 8 7 9 8.7.9 [1.17.38.11] CARLDGHFDLW in-frame + Low V-REGION identity (80,84% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctgggttcaccatcagtcactactccatggcctgggtccgccaggctccgggaaaggggctggagtgggtctcattaatttatcccggcgggagcacattccacgcagactccgtgagggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgtgcgagactcgacgggcacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcgggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 94 [274, 273] g88>a,V30>I(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g|a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c222>g|a225>g|t226>c,F76>L(+ - -)|c228>t,F76>L(+ - -)|c230>t,T77>I(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| g88>a,V30>I(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g| a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c222>g|a225>g|t226>c,F76>L(+ - -)|c228>t,F76>L(+ - -)|c230>t,T77>I(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| g319>c|a320>t| 318 (320) 220 (222) 179 41 (43) 9 (11) 32 9 8 5 3 3 2 2 (3) 2 2 (3) 3 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 2 0 0 2 0 1 0 0 0 0 0 51 51 45 6 2 4 2 1 0 0 0 0 0 1 1 0 1 0 30 21 17 4 2 2 0 0 0 1 1 0 0 0 1 0 0 1 117 114 88 26 5 21 7 5 5 2 0 2 1 1 0 3 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcaccatcagtcactactcc atggcctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgagggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc ARLDGHFDL IGHV3-53 IGHD6-6 IGHJ2 gggttcaccatcagtcactactcc atggcctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgagggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc IGG1 41 210 25 16 13 21 12 20 7 2 25 7 JY8QFUQ01A1GZY 5314 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 856 88.13 193/219 nt 87.67 192/219 nt Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD6-19*01 F 1 7 8 17 7.8.17 [2.17.38.11] CARDSASSGYARYDAMDVW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 29 of V-REGION: 1 nucleotides (from position 15 in the user submitted sequence), (cause frameshift) agcctctggattcagtttagtacacatggcatgaactgggtccgccaggctccagggaaggggccggaatgggtctcattcgttaatagtggaagtagttacatctactacgcagactcagtgaggggccgattcaccatctccagagacgacgccaggaattcactgtatctgcaaatgcaccgcctgcgagtcgaggacacggctctctactattgtgcgagagacagtgcctcgtccggctacgctaggtacgacgctatggacgtctggggccaagggaccgcggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [302] c87>g|c90>t|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|t109>c,Y37>H(+ - -)|a112>g,S38>G(+ + -)|t149>c,L50>P(- - -)|g153>a|c164>t,S55>F(- - -)|a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|a195>c|a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|a251>g,K84>R(+ + +)|c255>t|a274>c,N92>H(- - -)|a277>c,S93>R(- - -)|a283>c|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t306>c|c309>t| c87>g|c90>t|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|t109>c,Y37>H(+ - -)|a112>g,S38>G(+ + -)| t149>c,L50>P(- - -)|g153>a|c164>t,S55>F(- - -)| a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|a195>c| a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|a251>g,K84>R(+ + +)|c255>t|a274>c,N92>H(- - -)|a277>c,S93>R(- - -)|a283>c|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t306>c|c309>t| 320 227 201 26 8 18 6 2 5 3 4 1 0 1 3 1 0 0 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 17 6 2 4 1 0 1 1 0 1 0 0 1 1 0 0 51 51 48 3 1 2 0 1 1 1 0 0 0 0 0 0 0 0 30 24 19 5 1 4 2 1 0 0 1 0 0 1 0 0 0 0 117 114 102 12 4 8 3 0 3 1 3 0 0 0 2 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct ggattcagtttagtacacatggc atgaactgggtccgccaggctccagggaaggggccggaatgggtctcattc gttaatagtggaagtagttacatc tactacgcagactcagtgaggggccgattcaccatctccagagacgacgccaggaattcactgtatctgcaaatgcaccgcctgcgagtcgaggacacggctctctactattgt gcgagagacagtgcctcgtccggctacgctaggtacgacgctatggacgtc ARDSASSGYARYDAMDV IGHV3-21 IGHD6-19 IGHJ6 ggattcagtttagtacacatggc atgaactgggtccgccaggctccagggaaggggccggaatgggtctcattc gttaatagtggaagtagttacatc tactacgcagactcagtgaggggccgattcaccatctccagagacgacgccaggaattcactgtatctgcaaatgcaccgcctgcgagtcgaggacacggctctctactattgt gcgagagacagtgcctcgtccggctacgctaggtacgacgctatggacgtc IGG2 26 212 16 10 7 12 9 14 5 3 10 8 JY8QFUQ01A1ISV 21749 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 986 94.95 207/218 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD5-12*01 F 1 8 8 14 8.8.14 [1.17.38.11] CAKDLFQVSTTYFDYW in-frame - cctctggattcaccttcagtgactatcacatgtactgggtccgccaggctccaggcaaggggctggagtgggtggcagttatatcatatgatggaagtaataaatactatgtagactccgtgaagggccgattcaccatctccagagacaattccaagaatgcgctgtttctgcagatgaacagcctgagagctgacgacacggctgtgtattactgtgcgaaagacctctttcaggtgtctacgacttactttgactactggggccggggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [292] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g112>c,G38>H(+ - -)|g113>a,G38>H(+ - -)|c118>t,H40>Y(+ - -)|c203>t,A68>V(+ - +)|c255>t|a256>g,T86>A(- - -)|a263>t,Y88>F(- + -)|a270>g|g291>c,E97>D(+ - +)|g317>a,R106>K(+ + +)| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g112>c,G38>H(+ - -)|g113>a,G38>H(+ - -)| c118>t,H40>Y(+ - -)| c203>t,A68>V(+ - +)|c255>t|a256>g,T86>A(- - -)|a263>t,Y88>F(- + -)|a270>g|g291>c,E97>D(+ - +)| g317>a,R106>K(+ + +)| 320 226 214 12 2 10 3 3 3 0 0 0 1 0 2 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 2 0 0 0 0 0 0 1 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 2 4 2 0 2 0 0 0 1 0 1 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtgactatcac atgtactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcatatgatggaagtaataaa tactatgtagactccgtgaagggccgattcaccatctccagagacaattccaagaatgcgctgtttctgcagatgaacagcctgagagctgacgacacggctgtgtattactgt gcgaaagacctctttcaggtgtctacgacttactttgactac AKDLFQVSTTYFDY IGHV3-30 IGHD5-12 IGHJ4 ggattcaccttcagtgactatcac atgtactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcatatgatggaagtaataaa tactatgtagactccgtgaagggccgattcaccatctccagagacaattccaagaatgcgctgtttctgcagatgaacagcctgagagctgacgacacggctgtgtattactgt gcgaaagacctctttcaggtgtctacgacttactttgactac IGG1 11 213 8 3 5 7 3 4 2 0 5 4 JY8QFUQ01A1IV8 11763 productive (see comment) Homsap IGHV4-4*07 F 849 88.79 190/214 nt 87.85 188/214 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-19*01 F 1 8 7 11 8.7.11 [1.17.37.11] CARSGWDNQFDFW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'TGGCAC' (6 nucleotides at position 23 to 28 in the user submitted sequence), (do not cause frameshift); in FR3-IMGT, from codon 68 of V-REGION: 1 nucleotides (from position 110 in the user submitted sequence), (cause frameshift) tctctggtgtcgccaccagtagTGGCACttactactggagctggatccggcagtccgccggggcgggactagagtggattgggcgcatctataccggtcacaccaccatttacaaccctccctcaagggtcgagtcaccatgtcacttgacatgtccaagaaccagatctccctgaggctgacctctgtgaccgccgcagatacggccgtgtattactgtgcgcgaagtggctgggacaatcaatttgacttctggggccagggaaccctggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG4 100 88 [285] g83>t,G28>V(- - -)|t85>g,S29>A(- + -)|t89>c,I30>T(- - -)|c133>t,P45>S(+ - -)|a142>g,K48>A(- - -)|a143>c,K48>A(- - -)|g150>a|t165>c|a175>g,S59>G(+ + -)|g187>c,G63>H(+ - -)|g188>a,G63>H(+ - -)|g189>c,G63>H(+ - -)|g191>c,S64>T(+ - +)|a197>t,N66>I(- - -)|c198>t,N66>I(- - -)|a220>g,S74>G(+ + -)|g238>c,V80>L(+ - +)|a240>t,V80>L(+ - +)|c245>t,T82>M(- - -)|t259>a,F87>I(+ - -)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|g291>a|c294>t|a316>c| g83>t,G28>V(- - -)|t85>g,S29>A(- + -)|t89>c,I30>T(- - -)| c133>t,P45>S(+ - -)|a142>g,K48>A(- - -)|a143>c,K48>A(- - -)|g150>a|t165>c| a175>g,S59>G(+ + -)|g187>c,G63>H(+ - -)|g188>a,G63>H(+ - -)|g189>c,G63>H(+ - -)|g191>c,S64>T(+ - +)| a197>t,N66>I(- - -)|c198>t,N66>I(- - -)|a220>g,S74>G(+ + -)|g238>c,V80>L(+ - +)|a240>t,V80>L(+ - +)|c245>t,T82>M(- - -)|t259>a,F87>I(+ - -)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|g291>a|c294>t| a316>c|g319>a|a320>g| 318 (320) 220 (222) 195 25 (27) 5 (7) 20 4 (5) 3 (4) 4 2 2 0 2 1 5 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 1 0 0 0 0 0 0 1 1 51 51 46 5 2 3 1 1 1 1 1 0 0 0 0 0 0 0 30 21 16 5 0 5 1 1 0 0 0 0 0 0 3 0 0 0 117 113 102 11 2 9 2 1 3 0 0 0 2 1 2 0 0 0 6 (8) 6 5 1 (3) 1 (3) 0 0 (1) 0 (1) 0 0 1 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgtcgccaccagtagttactac tggagctggatccggcagtccgccggggcgggactagagtggattgggcgc atctataccggtcacaccacc atttacaaccctccctcaagggtcgagtcaccatgtcacttgacatgtccaagaaccagatctccctgaggctgacctctgtgaccgccgcagatacggccgtgtattactgt gcgcgaagtggctgggacaatcaatttgacttc ARSGWDNQFDF IGHV4-4 IGHD6-19 IGHJ4 ggtgtcgccaccagtagttactac tggagctggatccggcagtccgccggggcgggactagagtggattgggcgc atctataccggtcacaccacc atttacaaccctccctcaagggtcgagtcaccatgtcacttgacatgtccaagaaccagatctccctgaggctgacctctgtgaccgccgcagatacggccgtgtattactgt gcgcgaagtggctgggacaatcaatttgacttc IGG4 24 209 13 11 7 13 6 11 4 0 12 8 JY8QFUQ01A1IYG 10908 productive Homsap IGHV3-66*01 F, or Homsap IGHV3-66*02 F or Homsap IGHV3-66*04 F 948 93.93 201/214 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD4-23*01 ORF 2 8 7 10 8.7.10 [1.17.38.11] CARGDGVKDDYW in-frame + ctctggattcaccgtcaatagaaactacatgagctgggtccgccaggctccagggaagggactggagtgggtctcagttatttccagcggtggttccacatactacgcaaactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtatatcttcaaatgaacagcctgagagtcgacgacacggctgtgtattactgtgcgagaggggacggggttaaggatgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [276] g104>a,S35>N(- - -)|c108>a,S36>R(- - -)|g147>a|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a190>t|g191>c|g205>a,D69>N(+ + -)|a223>c|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| g104>a,S35>N(- - -)|c108>a,S36>R(- - -)| g147>a| a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a190>t|g191>c| g205>a,D69>N(+ + -)|a223>c|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| a320>g| 319 (320) 221 (222) 208 13 (14) 4 (5) 9 0 (1) 4 1 1 2 1 1 0 2 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 1 0 0 0 0 0 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 21 17 4 2 2 0 0 0 1 1 0 1 0 1 0 0 0 117 114 108 6 1 5 0 2 1 0 1 0 0 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|ggca,220-223(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccgtcaatagaaactac atgagctgggtccgccaggctccagggaagggactggagtgggtctcagtt atttccagcggtggttccaca tactacgcaaactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtatatcttcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaggggacggggttaaggatgactac ARGDGVKDDY IGHV3-66 IGHD4-23 IGHJ4 ggattcaccgtcaatagaaactac atgagctgggtccgccaggctccagggaagggactggagtgggtctcagtt atttccagcggtggttccaca tactacgcaaactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtatatcttcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaggggacggggttaaggatgactac IGG1 13 210 6 7 5 9 1 4 2 2 5 4 JY8QFUQ01A1K37 6644 productive Homsap IGHV3-48*01 F, or Homsap IGHV3-48*02 F 999 95.85 208/217 nt NA Homsap IGHJ6*02 F 256 90.32 56/62 nt Homsap IGHD4-23*01 ORF 2 8 8 20 8.8.20 [1.17.38.11] CVRGDYGGAPGHYYDPYGLDVW in-frame + ctctggattcaccttcaggagttatatcatgaactgggtccgccaggctccagggaaggggctggagtggatttcatacattagtagtagtggtattatcatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgtgtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtctggggccaagggaccacggtcaaagtctcctcagcctccaccaagggcccatcggtcttcccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 94 [308, 309] t105>g,S35>R(- - -)|c108>t|g113>t,S38>I(- - -)|g157>a,V53>I(+ - +)|a184>g,S62>G(+ + -)|g188>t,S63>I(- - -)|c191>t,T64>I(- - -)|c287>t,A96>V(+ - +)|c309>t|c314>t,A105>V(+ - +)| t105>g,S35>R(- - -)|c108>t|g113>t,S38>I(- - -)| g157>a,V53>I(+ - +)| a184>g,S62>G(+ + -)|g188>t,S63>I(- - -)|c191>t,T64>I(- - -)| c287>t,A96>V(+ - +)|c309>t| c314>t,A105>V(+ - +)|a320>g| 319 (320) 224 (225) 214 10 (11) 2 (3) 8 1 (2) 1 5 0 0 0 0 0 0 0 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 1 0 0 0 0 0 0 0 1 1 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 1 0 0 0 0 0 0 0 1 0 117 114 112 2 1 1 0 0 2 0 0 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcaggagttatatc atgaactgggtccgccaggctccagggaaggggctggagtggatttcatac attagtagtagtggtattatcata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgt gtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtc VRGDYGGAPGHYYDPYGLDV IGHV3-48 IGHD4-23 IGHJ6 ggattcaccttcaggagttatatc atgaactgggtccgccaggctccagggaaggggctggagtggatttcatac attagtagtagtggtattatcata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgt gtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtc IGG1 9 213 6 3 5 7 1 2 1 1 2 5 JY8QFUQ01A1KQO 20871 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 896 90.37 197/218 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-9*01 F 2 8 8 10 8.8.10 [1.17.38.11] CVSDLAGQKGFW in-frame + cctcaggattcaccttcagtaggtactggatgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgtattaatgaagacggcagctacacagatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtttttgcaaatgaacagtctgagaggcgaggacacggctgtctattactgtgttagcgatttggctggtcagaaaggtttctggggccagggagtcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 71 95 [279, 281] t78>a|c108>g,S36>R(- - -)|c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|a263>t,Y88>F(- + -)|c265>t|c287>g,A96>G(- + -)|g303>c| t78>a| c108>g,S36>R(- - -)| c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|a263>t,Y88>F(- + -)|c265>t|c287>g,A96>G(- + -)|g303>c| c314>t,A105>V(+ - +)|a315>t,A105>V(+ - +)|a318>c,R106>S(- - -)| 320 226 202 24 6 18 2 3 4 4 1 1 3 2 2 2 0 0 78 5 4 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 49 2 0 2 0 0 1 1 0 0 0 0 0 0 0 0 30 24 16 8 3 5 1 2 0 2 0 0 1 1 1 0 0 0 117 114 105 9 2 7 1 1 2 1 0 1 1 0 1 1 0 0 8 8 5 3 0 3 0 0 1 0 1 0 1 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctca ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagctacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtttttgcaaatgaacagtctgagaggcgaggacacggctgtctattactgt gttagcgatttggctggtcagaaaggtttc VSDLAGQKGF IGHV3-74 IGHD3-9 IGHJ4 ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagctacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtttttgcaaatgaacagtctgagaggcgaggacacggctgtctattactgt gttagcgatttggctggtcagaaaggtttc IGA2 20 213 12 8 6 11 6 9 2 3 9 6 JY8QFUQ01A1L2W 7478 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-2*01 F 2 8 8 22 8.8.22 [1.17.38.11] CAKDRDPGYCLSNSCVDGLAFDSW in-frame + ctctggattcacctttagcaactatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtctccggtattagtggtagtggtggtaacacatacttcgcagactccgtgaagggccggttcaccatctccagagacaattccaggaacacgctgtttctacagctgaacagcctgagagccgccgacacggccgtatattactgtgcgaaggatcgtgacccgggatattgtcttagtaatagctgtgttgacggcctagcctttgactcctggggcctgggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttc NA IGA1 100 100 [314] g107>a,S36>N(- - -)|a162>c|c164>g,A55>G(- + -)|g191>a,S64>N(- - -)|a200>t,Y67>F(- + -)|a251>g,K84>R(+ + +)|a263>t,Y88>F(- + -)|g267>a|a270>g|a271>c,M91>L(+ + -)|a290>c,E97>A(- - -)|g291>c,E97>A(- - -)|a318>g| g107>a,S36>N(- - -)| a162>c|c164>g,A55>G(- + -)| g191>a,S64>N(- - -)| a200>t,Y67>F(- + -)|a251>g,K84>R(+ + +)|a263>t,Y88>F(- + -)|g267>a|a270>g|a271>c,M91>L(+ + -)|a290>c,E97>A(- - -)|g291>c,E97>A(- - -)| a318>g| 320 225 212 13 4 9 3 3 0 0 3 0 2 0 1 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 0 0 0 1 0 0 0 0 1 0 0 30 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 117 114 106 8 2 6 2 1 0 0 2 0 2 0 1 0 0 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctccggt attagtggtagtggtggtaacaca tacttcgcagactccgtgaagggccggttcaccatctccagagacaattccaggaacacgctgtttctacagctgaacagcctgagagccgccgacacggccgtatattactgt gcgaaggatcgtgacccgggatattgtcttagtaatagctgtgttgacggcctagcctttgactcc AKDRDPGYCLSNSCVDGLAFDS IGHV3-23 IGHD2-2 IGHJ4 ggattcacctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctccggt attagtggtagtggtggtaacaca tacttcgcagactccgtgaagggccggttcaccatctccagagacaattccaggaacacgctgtttctacagctgaacagcctgagagccgccgacacggccgtatattactgt gcgaaggatcgtgacccgggatattgtcttagtaatagctgtgttgacggcctagcctttgactcc IGA1 12 213 5 7 3 5 2 7 3 0 7 2 JY8QFUQ01A1LNA 3381 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 954 93.55 203/217 nt 93.09 202/217 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-3*01 F 3 8 8 11 8.8.11 [1.17.38.11] CARFGPLTRPDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'G' (1 nucleotides at position 8 to 8 in the user submitted sequence), (cause frameshift); ctctggaGttcacctttatcaactatggcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtggtgataccacataccacgcagactccgtgcagggccgattcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagtcgaggacacggccgtttattactgtgcgagattcggcccacttactcgacctgactactggggccagggaactttggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtctagggcttcttccccca NA IGA2 79 95 [282, 281] g104>t,S35>I(- - -)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)|c164>g,A55>G(- + -)|g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)|t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|g225>a|t246>c|g258>t|c279>t|c287>t,A96>V(+ - +)|a303>t| g104>t,S35>I(- - -)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)| c164>g,A55>G(- + -)| g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)| t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|g225>a|t246>c|g258>t|c279>t|c287>t,A96>V(+ - +)|a303>t| a317>g,K106>R(+ + +)|g319>t|a320>t| 316 (320) 221 (225) 207 (208) 14 (17) 5 (7) 9 (10) 0 (1) 3 2 2 1 0 1 (2) 0 1 2 2 (3) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 0 1 1 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 117 114 106 8 5 3 0 1 2 2 1 0 1 0 0 0 1 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttatcaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgataccaca taccacgcagactccgtgcagggccgattcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagtcgaggacacggccgtttattactgt gcgagattcggcccacttactcgacctgactac ARFGPLTRPDY IGHV3-23 IGHD3-3 IGHJ4 ggattcacctttatcaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgataccaca taccacgcagactccgtgcagggccgattcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagtcgaggacacggccgtttattactgt gcgagattcggcccacttactcgacctgactac IGA2 14 213 7 7 5 10 2 4 5 0 4 5 JY8QFUQ01A1MBV 16997 productive (see comment) Homsap IGHV3-9*01 F 892 89.95 197/219 nt 89.5 196/219 nt Homsap IGHJ6*02 F (see comment) 175 75.81 47/62 nt Homsap IGHD1-1*01 F 1 8 8 15 8.8.15 [2.17.38.11] CAKDVTSESHFFALDVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 41 of V-REGION: 'G' (1 nucleotides at position 38 to 38 in the user submitted sequence), (cause frameshift); gcctcgggattcaagtttgatgaatatgccatgcactGgggtccggcgagttccagggaggggcctggagtgggtctcagttataagttgggatggtgatgacatcgcctatgcggactctgtgaagggccgattcagcatctccagagacaacgccaagaactccctgtacctggaaatgagcagtctgagagttgaggacacggccttctattactgtgcaaaggatgtcacatctgaaagtcacttcttcgctttggacgtctggggccaagggaccgcggtcaacgtctctccagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [296] t78>g|c86>a,T29>K(- - -)|c87>g,T29>K(- - -)|t108>a,D36>E(+ - +)|a131>g,Q44>R(+ - -)|c134>t,A45>V(+ - +)|a143>g,K48>R(+ + +)|g164>t,G55>V(- - -)|t168>a|a175>g,N59>D(+ + -)|a184>g,S62>G(+ + -)|g188>a,G63>D(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a195>c|g197>c,G66>A(- + -)|c230>g,T77>S(+ - +)|t264>c|c268>g,Q90>E(+ + -)|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|g303>c,L101>F(+ - -)|a318>g| t78>g| c86>a,T29>K(- - -)|c87>g,T29>K(- - -)|t108>a,D36>E(+ - +)| a131>g,Q44>R(+ - -)|c134>t,A45>V(+ - +)|a143>g,K48>R(+ + +)|g164>t,G55>V(- - -)| t168>a|a175>g,N59>D(+ + -)|a184>g,S62>G(+ + -)|g188>a,G63>D(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a195>c| g197>c,G66>A(- + -)|c230>g,T77>S(+ - +)|t264>c|c268>g,Q90>E(+ + -)|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|g303>c,L101>F(+ - -)| a318>g|a322>g| 321 (322) 228 (229) 205 23 (24) 5 (6) 18 7 (8) 2 2 1 1 1 0 2 2 3 1 1 78 6 5 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 36 24 21 3 0 3 0 0 0 0 0 1 0 1 0 1 0 0 51 51 47 4 0 4 2 0 1 0 0 0 0 0 0 0 1 0 30 24 17 7 2 5 3 2 0 0 1 0 0 1 0 0 0 0 117 114 107 7 1 6 1 0 1 1 0 0 0 0 2 2 0 0 9 (10) 9 8 1 (2) 1 (2) 0 1 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| gcctcg ggattcaagtttgatgaatatgcc atgcactgggtccggcgagttccagggaggggcctggagtgggtctcagtt ataagttgggatggtgatgacatc gcctatgcggactctgtgaagggccgattcagcatctccagagacaacgccaagaactccctgtacctggaaatgagcagtctgagagttgaggacacggccttctattactgt gcaaaggatgtcacatctgaaagtcacttcttcgctttggacgtc AKDVTSESHFFALDV IGHV3-9 IGHD1-1 IGHJ6 ggattcaagtttgatgaatatgcc atgcactgggtccggcgagttccagggaggggcctggagtgggtctcagtt ataagttgggatggtgatgacatc gcctatgcggactctgtgaagggccgattcagcatctccagagacaacgccaagaactccctgtacctggaaatgagcagtctgagagttgaggacacggccttctattactgt gcaaaggatgtcacatctgaaagtcacttcttcgctttggacgtc IGA1 21 213 11 10 4 11 7 10 1 2 10 8 JY8QFUQ01A1MJG 17281 productive (see comment) Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F 903 91.59 196/214 nt 91.12 195/214 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD5-24*01 ORF 1 8 7 17 8.7.17 [1.17.38.11] CARVVMATVYTGSYYFDDW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 48 to 48 in the user submitted sequence), (cause frameshift); gtatggtgggtccttcagtgataactcctggacctggatccgccagcCccccagggaaggggctggagtggattggagagatcaatcatagtggaaacaccaactacaacccgtccctcaggagtcgagtaatcatgtcaatagacacgtccatgaaccaattctccctgaagctgacttctgtgaccgccgcggacacggctgtgtattattgtgcgagagtcgtgatggctacagtttatactgggtcctactactttgacgactggggccagggaaccctggtctccgtcttctcggcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtgtcatcgcctgcctggtcagggcttcttccc NA IGA1 93 89 [297] c75>g|g107>a,G36>D(- - -)|t109>a,Y37>N(- - -)|a113>c,Y38>S(+ - -)|g119>c,S40>T(+ - +)|g162>a|a165>g|g191>a,S64>N(- - -)|a215>g,K72>R(+ + +)|c228>a|c230>t,T77>I(- - -)|a234>g,I78>M(+ + -)|g238>a,V80>I(+ - +)|a251>t,K84>M(- + -)|g258>a|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|c309>t| c75>g| g107>a,G36>D(- - -)|t109>a,Y37>N(- - -)|a113>c,Y38>S(+ - -)| g119>c,S40>T(+ - +)|g162>a|a165>g| g191>a,S64>N(- - -)| a215>g,K72>R(+ + +)|c228>a|c230>t,T77>I(- - -)|a234>g,I78>M(+ + -)|g238>a,V80>I(+ - +)|a251>t,K84>M(- + -)|g258>a|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|c309>t| g320>t| 319 (320) 221 (222) 203 18 (19) 6 (7) 12 3 5 3 0 1 1 1 1 2 1 0 (1) 0 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 21 3 0 3 0 1 0 0 1 0 0 1 0 0 0 0 51 51 48 3 2 1 1 1 0 0 0 0 0 0 1 0 0 0 30 21 20 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 117 114 104 10 3 7 2 2 3 0 0 1 1 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 0 0 (1) 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtat ggtgggtccttcagtgataactcc tggacctggatccgccagcccccagggaaggggctggagtggattggagag atcaatcatagtggaaacacc aactacaacccgtccctcaggagtcgagtaatcatgtcaatagacacgtccatgaaccaattctccctgaagctgacttctgtgaccgccgcggacacggctgtgtattattgt gcgagagtcgtgatggctacagtttatactgggtcctactactttgacgac ARVVMATVYTGSYYFDD IGHV4-34 IGHD5-24 IGHJ4 ggtgggtccttcagtgataactcc tggacctggatccgccagcccccagggaaggggctggagtggattggagag atcaatcatagtggaaacacc aactacaacccgtccctcaggagtcgagtaatcatgtcaatagacacgtccatgaaccaattctccctgaagctgacttctgtgaccgccgcggacacggctgtgtattattgt gcgagagtcgtgatggctacagtttatactgggtcctactactttgacgac IGA1 17 210 11 6 8 11 3 6 5 0 8 4 JY8QFUQ01A1MJU 18347 productive (see comment) Homsap IGHV3-9*01 F 931 92.59 200/216 nt 91.2 197/216 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 2 8 8 16 8.8.16 [1.17.38.11] CAKDGSAIPVTGTYFEYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'G' (1 nucleotides at position 13 to 13 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 74 of V-REGION: 'G' (1 nucleotides at position 125 to 125 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 79 of V-REGION: 'C' (1 nucleotides at position 143 to 143 in the user submitted sequence), (cause frameshift); tctggattcaccGtttcatgattataccatgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagtattagttggaatagtggtaacatagactatgcggcctctgtgaagGggccgattcaccgtctcCcagagacaacgccaataactccctgtctcttcagatgaatggtctgagatctgaggacacggccctctattactgtgcaaaagacgggtcggcgataccagtgactggtacctactttgaatactggggccagggagccctggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG4 100 88 [299] g103>c,D35>H(- - -)|g112>a,A38>T(- - -)|g163>a,G55>S(+ + -)|g191>a,S64>N(- - -)|g197>a,G66>D(- - -)|a206>c,D69>A(- - -)|a232>g,I78>V(+ - +)|g252>t,K84>N(+ - -)|a263>c,Y88>S(+ - -)|g267>t|a270>g|c276>t|a277>g,S93>G(+ + -)|g286>t,A96>S(- + -)|t301>c|g303>c| g103>c,D35>H(- - -)|g112>a,A38>T(- - -)| g163>a,G55>S(+ + -)| g191>a,S64>N(- - -)| g197>a,G66>D(- - -)|a206>c,D69>A(- - -)|a232>g,I78>V(+ - +)|g252>t,K84>N(+ - -)|a263>c,Y88>S(+ - -)|g267>t|a270>g|c276>t|a277>g,S93>G(+ + -)|g286>t,A96>S(- + -)|t301>c|g303>c| t321>c|a322>g| 320 (322) 224 (226) 208 16 (18) 5 (7) 11 3 (4) 4 1 1 (2) 2 0 0 0 2 0 3 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 117 114 102 12 5 7 3 1 1 1 2 0 0 0 1 0 3 0 8 (10) 8 8 0 (2) 0 (2) 0 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| tct ggattcacctttcatgattatacc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt attagttggaatagtggtaacata gactatgcggcctctgtgaagggccgattcaccgtctccagagacaacgccaataactccctgtctcttcagatgaatggtctgagatctgaggacacggccctctattactgt gcaaaagacgggtcggcgataccagtgactggtacctactttgaatac AKDGSAIPVTGTYFEY IGHV3-9 IGHD6-19 IGHJ4 ggattcacctttcatgattatacc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt attagttggaatagtggtaacata gactatgcggcctctgtgaagggccgattcaccgtctccagagacaacgccaataactccctgtctcttcagatgaatggtctgagatctgaggacacggccctctattactgt gcaaaagacgggtcggcgataccagtgactggtacctactttgaatac IGG4 16 213 9 7 5 10 4 6 5 0 8 3 JY8QFUQ01A1OLP 10211 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F (see comment) 743 82.57 180/218 nt 82.11 179/218 nt Homsap IGHJ3*01 F (see comment) 205 90 45/50 nt Homsap IGHD3-3*02 F 2 8 8 13 8.8.13 [1.17.38.11] CARIWDSWSGAFDVW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (82,57% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*03 and Homsap_IGHJ4*01 and Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT at codon 29 of V-REGION: 'C' (1 nucleotides at position 13 to 13 in the user submitted sequence), (cause frameshift); cctctggattccCcctttagtgtgtactggatgtactgggtccgccaaagtcccgggaaggggccggtatgggtcgcacgtatcagtgatgatggcaagagtatcagttatgcggactccgtgaggggccgattcaccatctctagggacaacgcccagaacacactgtctctgcaaatggacaatgtgagagccgacgactcgtctgtctattattgtgcaagaatttgggattcctggagtggagcttttgatgtgtggggccaagggacattggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [289] a85>c,T29>P(+ + -)|c90>t|a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|c108>g,S36>V(- - -)|c118>t,H40>Y(+ - -)|g133>a,A45>S(- + -)|c134>g,A45>S(- + -)|a138>c|t149>c,L50>P(- - -)|g153>a|t160>g,S54>A(- + -)|t168>c|a170>g,N57>S(- - -)|a172>g,S58>D(- - -)|g173>a,S58>D(- - -)|g186>c|g188>a,S63>K(- - -)|t189>g,S63>K(- - -)|c192>t|c194>t,T65>I(- - -)|a195>c,T65>I(- - -)|c198>t|c201>t|a215>g,K72>R(+ + +)|c237>t|a240>g|a250>c,K84>Q(+ - -)|g258>a|a263>c,Y88>S(+ - -)|a274>g,N92>D(+ + -)|g278>a,S93>N(- - -)|c280>g,L94>V(+ - +)|g291>c,E97>D(+ - +)|a295>t,T99>S(+ - +)|g298>t,A100>S(- + -)|g303>c|c309>t| a85>c,T29>P(+ + -)|c90>t|a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|c108>g,S36>V(- - -)| c118>t,H40>Y(+ - -)|g133>a,A45>S(- + -)|c134>g,A45>S(- + -)|a138>c|t149>c,L50>P(- - -)|g153>a|t160>g,S54>A(- + -)| t168>c|a170>g,N57>S(- - -)|a172>g,S58>D(- - -)|g173>a,S58>D(- - -)|g186>c|g188>a,S63>K(- - -)|t189>g,S63>K(- - -)|c192>t|c194>t,T65>I(- - -)|a195>c,T65>I(- - -)| c198>t|c201>t|a215>g,K72>R(+ + +)|c237>t|a240>g|a250>c,K84>Q(+ - -)|g258>a|a263>c,Y88>S(+ - -)|a274>g,N92>D(+ + -)|g278>a,S93>N(- - -)|c280>g,L94>V(+ - +)|g291>c,E97>D(+ - +)|a295>t,T99>S(+ - +)|g298>t,A100>S(- + -)|g303>c|c309>t| g319>a|a320>t| 318 (320) 224 (226) 186 38 (40) 13 (15) 25 6 6 (7) 8 2 5 0 1 (2) 0 3 3 2 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 1 0 1 0 1 0 0 0 0 1 1 0 51 51 44 7 2 5 0 2 1 1 1 0 0 0 0 1 0 1 30 24 14 10 3 7 2 2 2 1 1 0 0 0 1 0 0 1 117 114 98 16 7 9 3 2 4 0 2 0 1 0 2 1 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcccctttagtgtgtactgg atgtactgggtccgccaaagtcccgggaaggggccggtatgggtcgcacgt atcagtgatgatggcaagagtatc agttatgcggactccgtgaggggccgattcaccatctctagggacaacgcccagaacacactgtctctgcaaatggacaatgtgagagccgacgactcgtctgtctattattgt gcaagaatttgggattcctggagtggagcttttgatgtg ARIWDSWSGAFDV IGHV3-74 IGHD3-3 IGHJ3 ggattcccctttagtgtgtactgg atgtactgggtccgccaaagtcccgggaaggggccggtatgggtcgcacgt atcagtgatgatggcaagagtatc agttatgcggactccgtgaggggccgattcaccatctctagggacaacgcccagaacacactgtctctgcaaatggacaatgtgagagccgacgactcgtctgtctattattgt gcaagaatttgggattcctggagtggagcttttgatgtg IGA1 38 213 22 16 14 22 8 16 9 4 14 11 JY8QFUQ01A1PLD 16651 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 896 90.37 197/218 nt 89.91 196/218 nt Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-9*01 F 2 8 8 10 8.8.10 [1.17.38.11] CVSDLAGQKGFW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 41 of V-REGION: 'G' (1 nucleotides at position 37 to 37 in the user submitted sequence), (cause frameshift); cctcaggattcaccttcagtaggtactggatgcactGgggtccgccaagttccagggaaggggccggtgtgggtctcacgtattaatgaagacggcagctacacagatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtttttgcaaatgaacagtctgagaggcgaggacacggctgtctattactgtgttagcgatttggctggtcagaaaggtttctggggccagggagtcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA1 71 100 [280] t78>a|c108>g,S36>R(- - -)|c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|a263>t,Y88>F(- + -)|c265>t|c287>g,A96>G(- + -)|g303>c| t78>a| c108>g,S36>R(- - -)| c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|a263>t,Y88>F(- + -)|c265>t|c287>g,A96>G(- + -)|g303>c| c314>t,A105>V(+ - +)|a315>t,A105>V(+ - +)|a318>c,R106>S(- - -)| 320 226 202 24 6 18 2 3 4 4 1 1 3 2 2 2 0 0 78 5 4 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 49 2 0 2 0 0 1 1 0 0 0 0 0 0 0 0 30 24 16 8 3 5 1 2 0 2 0 0 1 1 1 0 0 0 117 114 105 9 2 7 1 1 2 1 0 1 1 0 1 1 0 0 8 8 5 3 0 3 0 0 1 0 1 0 1 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctca ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagctacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtttttgcaaatgaacagtctgagaggcgaggacacggctgtctattactgt gttagcgatttggctggtcagaaaggtttc VSDLAGQKGF IGHV3-74 IGHD3-9 IGHJ4 ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagctacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtttttgcaaatgaacagtctgagaggcgaggacacggctgtctattactgt gttagcgatttggctggtcagaaaggtttc IGA1 20 213 12 8 6 11 6 9 2 3 9 6 JY8QFUQ01A1Q3N 18544 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*02 F or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 797 85.32 186/218 nt NA Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F (see comment) 159 81.25 39/48 nt Homsap IGHD3-16*02 F 2 8 8 17 8.8.17 [1.17.38.11] CVKDRDFLWGSYRLQLESW in-frame + Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) cctctggattcacctttagtagctatggcatgagttgggtccgccagtctccaaataagggactggagtgggtcgcaggcattagtgcaaatggtggcagtataaattatctggacgccgtgaagggccggtttatcatctctagagacaattccaagaacacgttgtatctgcaaatggacagcctgacagtcgaggacacggccgtttattactgtgtgaaagatcgtgattttctttgggggagctatcgtcttcaacttgaatcttggggccaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 100 [300] c105>t|c113>g,A38>G(- + -)|c120>t|g133>t,A45>S(- + -)|g139>a,G47>N(- - -)|g140>a,G47>N(- - -)|g141>t,G47>N(- - -)|g147>a|t160>g,S54>A(- + -)|c164>g,A55>G(- + -)|t165>c,A55>G(- + -)|g173>c,G58>A(- + -)|t174>a,G58>A(- + -)|g176>a,S59>N(- - -)|t189>c|c192>t|c194>t,T65>I(- - -)|t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|c201>t|g202>c,A68>L(+ - +)|c203>t,A68>L(+ - +)|a204>g,A68>L(+ - +)|t208>g,S70>A(- + -)|c228>t|c230>t,T77>I(- - -)|c237>t|c259>t|a274>g,N92>D(+ + -)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|a303>t|c314>t,A105>V(+ - +)| c105>t|c113>g,A38>G(- + -)| c120>t|g133>t,A45>S(- + -)|g139>a,G47>N(- - -)|g140>a,G47>N(- - -)|g141>t,G47>N(- - -)|g147>a|t160>g,S54>A(- + -)|c164>g,A55>G(- + -)|t165>c,A55>G(- + -)| g173>c,G58>A(- + -)|t174>a,G58>A(- + -)|g176>a,S59>N(- - -)|t189>c|c192>t|c194>t,T65>I(- - -)| t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|c201>t|g202>c,A68>L(+ - +)|c203>t,A68>L(+ - +)|a204>g,A68>L(+ - +)|t208>g,S70>A(- + -)|c228>t|c230>t,T77>I(- - -)|c237>t|c259>t|a274>g,N92>D(+ + -)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|a303>t| c314>t,A105>V(+ - +)| 320 226 193 33 10 23 2 4 13 2 0 0 1 2 3 2 2 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 1 0 0 0 0 0 0 1 0 0 51 51 42 9 2 7 0 3 1 1 0 0 0 0 0 1 2 1 30 24 18 6 2 4 0 1 2 1 0 0 0 1 1 0 0 0 117 114 99 15 5 10 2 0 8 0 0 0 1 1 2 0 0 1 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagtagctatggc atgagttgggtccgccagtctccaaataagggactggagtgggtcgcaggc attagtgcaaatggtggcagtata aattatctggacgccgtgaagggccggtttatcatctctagagacaattccaagaacacgttgtatctgcaaatggacagcctgacagtcgaggacacggccgtttattactgt gtgaaagatcgtgattttctttgggggagctatcgtcttcaacttgaatct VKDRDFLWGSYRLQLES IGHV3-23 IGHD3-16 IGHJ4 ggattcacctttagtagctatggc atgagttgggtccgccagtctccaaataagggactggagtgggtcgcaggc attagtgcaaatggtggcagtata aattatctggacgccgtgaagggccggtttatcatctctagagacaattccaagaacacgttgtatctgcaaatggacagcctgacagtcgaggacacggccgtttattactgt gtgaaagatcgtgattttctttgggggagctatcgtcttcaacttgaatct IGA1 32 213 20 12 16 23 4 9 7 3 17 5 JY8QFUQ01A1QLN 6643 productive (see comment) Homsap IGHV3-13*01 F 971 94.88 204/215 nt 94.42 203/215 nt Homsap IGHJ5*02 F 255 100 51/51 nt Homsap IGHD3-9*01 F 1 8 7 13 8.7.13 [2.17.37.11] CARGSSKAYNWFDPW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 97 of V-REGION: 1 nucleotides (from position 193 in the user submitted sequence), (cause frameshift) gcctctggattcacgttcagtaattacgacatgcactgggtccgccaacctagaggaagaggtctggagtgggtctcagctattggcactggtggtgacacatactatccagactccgtgaagggccgattcaccatctccagagaaaatgccaagaactccttatatcttcagatgaacagcctgagagccgggacacggctgtgtattactgtgcaagagggtcgtcaaaagcgtacaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [286] c87>g|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g133>c,A45>P(- - -)|c137>g,T46>R(- - -)|a143>g,K48>R(+ + +)|t171>c|c176>g,A59>G(- + -)|g206>a,G69>D(- - -)|g261>a|a270>g| c87>g|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g133>c,A45>P(- - -)|c137>g,T46>R(- - -)|a143>g,K48>R(+ + +)| t171>c|c176>g,A59>G(- + -)| g206>a,G69>D(- - -)|g261>a|a270>g| a320>g| 319 (320) 222 (223) 211 11 (12) 4 (5) 7 2 (3) 3 1 1 0 0 0 0 1 3 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 0 0 0 0 0 0 1 0 0 51 51 48 3 0 3 1 0 0 0 0 0 0 0 1 1 0 0 30 21 19 2 1 1 0 0 0 1 0 0 0 0 0 1 0 0 117 113 110 3 2 1 1 2 0 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|ta,135-136(FR2)|aa,141-142(FR2)|aa,143-144(FR2)|ta,165-166(FR2-CDR2)|ta,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,242-243(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,135-136(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,259-260(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|agct,162-165(FR2)|ggta,169-172(CDR2)|ggct,205-208(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,162-165(FR2)|tact,171-174(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacgttcagtaattacgac atgcactgggtccgccaacctagaggaagaggtctggagtgggtctcagct attggcactggtggtgacaca tactatccagactccgtgaagggccgattcaccatctccagagaaaatgccaagaactccttatatcttcagatgaacagcctgagagccgggacacggctgtgtattactgt gcaagagggtcgtcaaaagcgtacaactggttcgacccc ARGSSKAYNWFDP IGHV3-13 IGHD3-9 IGHJ5 ggattcacgttcagtaattacgac atgcactgggtccgccaacctagaggaagaggtctggagtgggtctcagct attggcactggtggtgacaca tactatccagactccgtgaagggccgattcaccatctccagagaaaatgccaagaactccttatatcttcagatgaacagcctgagagccgggacacggctgtgtattactgt gcaagagggtcgtcaaaagcgtacaactggttcgacccc IGG1 11 209 7 4 4 8 3 3 2 2 4 3 JY8QFUQ01A1R7K 14002 productive (see comment) Homsap IGHV1-2*02 F, or Homsap IGHV1-2*04 F 826 87.79 187/213 nt 87.32 186/213 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD6-19*01 F 1 7 8 12 7.8.12 [0.17.38.11] CARGPVSGWYGDFW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 35 of V-REGION: 1 nucleotides (from position 15 in the user submitted sequence), (cause frameshift) tggatacatcttcaaaactactatatattctgggtgcgacgggcccctggacaagggcttgagtggatgggttgggtcaaccctaacagtggtgccacacactatgcaccgaaatatcagggccgggtcaccatgaccagggacacgtcattcgccacagcctacatggatttgagcatgttgacatctgacgacacggccatgtattactgtgcgagaggccctgtcagtggttggtatggtgacttctggggccagggaaccctagtcaacgtctcatcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [280] c86>t,T29>I(- - -)|c105>a|g106>a,G36>N(- - -)|g107>a,G36>N(- - -)|g117>a,M39>I(+ + -)|c118>t,H40>F(- - -)|a119>t,H40>F(- - -)|a131>g,Q44>R(+ - -)|a162>t|a166>g,I56>V(+ - +)|g191>c,G64>A(- + -)|a196>c,N66>H(- - -)|a206>c,Q69>P(- - -)|g210>a|t212>a,F71>Y(- + -)|a223>c|c249>a|a250>t,I84>F(+ - -)|a253>g,S85>A(- + -)|g254>c,S85>A(- + -)|g270>t,E90>D(+ - +)|c271>t|g278>t,R93>M(- + -)|c280>t|g284>c,R95>T(- - -)|g301>a,V101>M(+ - -)| c86>t,T29>I(- - -)|c105>a|g106>a,G36>N(- - -)|g107>a,G36>N(- - -)| g117>a,M39>I(+ + -)|c118>t,H40>F(- - -)|a119>t,H40>F(- - -)|a131>g,Q44>R(+ - -)|a162>t| a166>g,I56>V(+ - +)|g191>c,G64>A(- + -)| a196>c,N66>H(- - -)|a206>c,Q69>P(- - -)|g210>a|t212>a,F71>Y(- + -)|a223>c|c249>a|a250>t,I84>F(+ - -)|a253>g,S85>A(- + -)|g254>c,S85>A(- + -)|g270>t,E90>D(+ - +)|c271>t|g278>t,R93>M(- + -)|c280>t|g284>c,R95>T(- - -)|g301>a,V101>M(+ - -)| a320>g| 319 (320) 220 (221) 194 26 (27) 7 (8) 19 3 (4) 5 4 0 3 2 3 1 3 0 2 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 19 4 1 3 0 2 1 0 0 1 0 0 0 0 0 0 51 51 46 5 1 4 1 1 1 0 0 0 2 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 117 114 99 15 5 10 1 2 2 0 3 1 1 1 2 0 2 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatacatcttcaaaactactat atattctgggtgcgacgggcccctggacaagggcttgagtggatgggttgg gtcaaccctaacagtggtgccaca cactatgcaccgaaatatcagggccgggtcaccatgaccagggacacgtcattcgccacagcctacatggatttgagcatgttgacatctgacgacacggccatgtattactgt gcgagaggccctgtcagtggttggtatggtgacttc ARGPVSGWYGDF IGHV1-2 IGHD6-19 IGHJ4 ggatacatcttcaaaactactat atattctgggtgcgacgggcccctggacaagggcttgagtggatgggttgg gtcaaccctaacagtggtgccaca cactatgcaccgaaatatcagggccgggtcaccatgaccagggacacgtcattcgccacagcctacatggatttgagcatgttgacatctgacgacacggccatgtattactgt gcgagaggccctgtcagtggttggtatggtgacttc IGA1 26 212 12 14 9 16 3 10 6 1 14 5 JY8QFUQ01A1RAE 10165 productive Homsap IGHV3-30*01 F 936 92.63 201/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-15*01 F 2 8 8 13 8.8.13 [1.17.38.11] CPKTGPGHSSPFDYW in-frame + ctctggattcaccttccgaagttatgatctgcactgggtccgccaggctccaggcaaggggctagagtgggtggcatttatttcaaacgatggaagtgacacagactacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaagacgctatatctgcaaatgaacagcctgagagttgaggacacggctgtgtattactgtccgaaaaccggccccggtcatagcagcccctttgactactggggccagggaaccctggtcagcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] a103>c,S35>R(- - -)|t105>a,S35>R(- - -)|c108>t|c113>a,A38>D(- - -)|a115>c,M39>L(+ + -)|g163>t,V55>F(+ - -)|a168>t|t172>a,Y58>N(- - -)|t174>c,Y58>N(- - -)|a190>g,N64>D(+ + -)|t192>c,N64>D(+ + -)|a194>c,K65>T(- - -)|t196>g,Y66>D(- - -)|c255>g,N85>K(+ - -)|g261>a|c287>t,A96>V(+ - +)|g313>c,A105>P(- - -)| a103>c,S35>R(- - -)|t105>a,S35>R(- - -)|c108>t|c113>a,A38>D(- - -)| a115>c,M39>L(+ + -)|g163>t,V55>F(+ - -)| a168>t|t172>a,Y58>N(- - -)|t174>c,Y58>N(- - -)|a190>g,N64>D(+ + -)|t192>c,N64>D(+ + -)|a194>c,K65>T(- - -)| t196>g,Y66>D(- - -)|c255>g,N85>K(+ - -)|g261>a|c287>t,A96>V(+ - +)| g313>c,A105>P(- - -)|g317>a,R106>K(+ + +)|g319>a|a320>c| 316 (320) 221 (225) 204 (205) 17 (20) 3 (5) 14 (15) 1 1 (3) 2 2 3 (4) 1 1 2 1 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 1 0 1 1 0 1 0 0 0 0 51 51 49 2 0 2 0 0 0 0 1 0 0 0 0 0 1 0 30 24 18 6 1 5 1 0 0 2 1 0 1 1 0 0 0 0 117 114 110 4 1 3 0 1 1 0 0 0 0 0 0 1 0 1 4 (8) 4 3 (4) 1 (4) 0 (2) 1 (2) 0 0 (2) 0 0 0 (1) 0 0 0 1 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttccgaagttatgat ctgcactgggtccgccaggctccaggcaaggggctagagtgggtggcattt atttcaaacgatggaagtgacaca gactacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaagacgctatatctgcaaatgaacagcctgagagttgaggacacggctgtgtattactgt ccgaaaaccggccccggtcatagcagcccctttgactac PKTGPGHSSPFDY IGHV3-30 IGHD2-15 IGHJ4 ggattcaccttccgaagttatgat ctgcactgggtccgccaggctccaggcaaggggctagagtgggtggcattt atttcaaacgatggaagtgacaca gactacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaagacgctatatctgcaaatgaacagcctgagagttgaggacacggctgtgtattactgt ccgaaaaccggccccggtcatagcagcccctttgactac IGA1 16 213 6 10 3 6 3 10 1 2 5 8 JY8QFUQ01A1SIW 13323 productive Homsap IGHV3-48*02 F, or Homsap IGHV3-48*04 F 944 93.49 201/215 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-18*01 F 3 8 8 13 8.8.13 [0.17.38.11] CARDRRGYSYEMDYW in-frame - ctggattcaccttcagtgtccatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcataccttagtagtggtagtgataccatatactacgcagactctgtgaggggccggttcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagtggcctgagagacgaggacacggctgtttattactgtgcgagagatcggcgtgggtatagttatgaaatggactactggggacagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [286] a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|t109>c,Y37>H(+ - -)|a166>c,I56>L(+ + +)|a175>g,S59>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a215>g,K72>R(+ + +)|a225>g|t246>c|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|a277>g,S93>G(+ + -)|g303>t| a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|t109>c,Y37>H(+ - -)| a166>c,I56>L(+ + +)|a175>g,S59>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)| a215>g,K72>R(+ + +)|a225>g|t246>c|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|a277>g,S93>G(+ + -)|g303>t| 320 223 209 14 3 11 7 1 1 2 1 0 0 0 0 0 2 0 78 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 0 0 1 0 0 0 0 0 0 1 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 2 1 0 0 1 0 0 0 0 0 0 0 117 114 107 7 3 4 4 0 1 1 0 0 0 0 0 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ct ggattcaccttcagtgtccatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac cttagtagtggtagtgataccata tactacgcagactctgtgaggggccggttcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagtggcctgagagacgaggacacggctgtttattactgt gcgagagatcggcgtgggtatagttatgaaatggactac ARDRRGYSYEMDY IGHV3-48 IGHD5-18 IGHJ4 ggattcaccttcagtgtccatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac cttagtagtggtagtgataccata tactacgcagactctgtgaggggccggttcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagtggcctgagagacgaggacacggctgtttattactgt gcgagagatcggcgtgggtatagttatgaaatggactac IGG1 14 213 11 3 2 4 9 10 3 0 4 7 JY8QFUQ01A1U7S 14609 productive (see comment) Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F (see comment) 669 79.44 170/214 nt 78.97 169/214 nt Homsap IGHJ6*02 F 229 85.48 53/62 nt Homsap IGHD3-10*01 F 2 8 8 21 8.8.21 [0.17.38.11] CARERRPGGYPNGYHHFYGMDVW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (79,44% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR2-IMGT at codon 64 of V-REGION: 'G' (1 nucleotides at position 96 to 96 in the user submitted sequence), (cause frameshift); ctctgatacaattagtcgttatggcatgcactgggtccgccaggctccaggcaaggggctggagtggctggcaattatttcatatgatggaaataGggatatatgttggagactccgtgaagggccgcttcaccgtttccagagacaacgccgggaggactctgtttctgcaaatgaacagtctgagaggtgatgacaccgccacatatttttgtgcgagagagcggcgtccgggtggctatccaaatggctaccaccatttttacggcatggacgtctggggccaagggaccagggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [310] t78>c|g79>t,G27>S(+ + -)|g80>c,G27>S(+ + -)|a81>t,G27>S(+ + -)|t82>g,F28>D(- - -)|t83>a,F28>D(- - -)|c84>t,F28>D(- - -)|c87>a|t88>a,F30>I(+ - -)|c90>t,F30>I(+ - -)|a106>c,S36>R(- - -)|c108>t,S36>R(- - -)|g157>c,V53>L(+ - +)|g163>a,V55>I(+ - +)|a168>t|g188>a,S63>N(- - -)|a191>g,N64>R(+ - -)|t192>g,N64>R(+ - -)|a194>t,K65>I(- + -)|c198>t|t199>g,Y67>V(- - -)|a200>t,Y67>V(- - -)|c203>g,A68>G(- + -)|a225>c|a232>g,I78>V(+ - +)|c234>t,I78>V(+ - +)|t246>c|t247>g,S83>A(- + -)|a250>g,K84>G(- - -)|a251>g,K84>G(- - -)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)|g258>t|a263>t,Y88>F(- + -)|c279>t|c287>g,A96>G(- + -)|g291>t,E97>D(+ - +)|g297>c|t300>c|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| t78>c| g79>t,G27>S(+ + -)|g80>c,G27>S(+ + -)|a81>t,G27>S(+ + -)|t82>g,F28>D(- - -)|t83>a,F28>D(- - -)|c84>t,F28>D(- - -)|c87>a|t88>a,F30>I(+ - -)|c90>t,F30>I(+ - -)|a106>c,S36>R(- - -)|c108>t,S36>R(- - -)| g157>c,V53>L(+ - +)|g163>a,V55>I(+ - +)| a168>t|g188>a,S63>N(- - -)|a191>g,N64>R(+ - -)|t192>g,N64>R(+ - -)|a194>t,K65>I(- + -)| c198>t|t199>g,Y67>V(- - -)|a200>t,Y67>V(- - -)|c203>g,A68>G(- + -)|a225>c|a232>g,I78>V(+ - +)|c234>t,I78>V(+ - +)|t246>c|t247>g,S83>A(- + -)|a250>g,K84>G(- - -)|a251>g,K84>G(- - -)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)|g258>t|a263>t,Y88>F(- + -)|c279>t|c287>g,A96>G(- + -)|g291>t,E97>D(+ - +)|g297>c|t300>c|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| 320 222 178 44 10 34 5 4 7 4 2 1 6 2 3 3 3 4 78 1 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 13 11 1 10 0 0 3 0 1 1 1 2 1 0 1 1 51 51 49 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 30 24 19 5 1 4 1 1 0 0 0 0 2 0 0 0 0 1 117 114 89 25 7 18 4 2 4 3 1 0 3 0 1 3 2 2 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| c tctgatacaattagtcgttatggc atgcactgggtccgccaggctccaggcaaggggctggagtggctggcaatt atttcatatgatggaaataggata tatgttggagactccgtgaagggccgcttcaccgtttccagagacaacgccgggaggactctgtttctgcaaatgaacagtctgagaggtgatgacaccgccacatatttttgt gcgagagagcggcgtccgggtggctatccaaatggctaccaccatttttacggcatggacgtc ARERRPGGYPNGYHHFYGMDV IGHV3-30 IGHD3-10 IGHJ6 tctgatacaattagtcgttatggc atgcactgggtccgccaggctccaggcaaggggctggagtggctggcaatt atttcatatgatggaaataggata tatgttggagactccgtgaagggccgcttcaccgtttccagagacaacgccgggaggactctgtttctgcaaatgaacagtctgagaggtgatgacaccgccacatatttttgt gcgagagagcggcgtccgggtggctatccaaatggctaccaccatttttacggcatggacgtc IGG1 43 213 19 24 11 21 8 22 7 2 20 14 JY8QFUQ01A1U87 21322 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 963 94.01 204/217 nt 93.55 203/217 nt Homsap IGHJ4*02 F 78 62.5 30/48 nt Homsap IGHD2-8*01 F 2 8 8 8 8.8.8 [1.17.38.11] CARDNYGDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 12 to 12 in the user submitted sequence), (cause frameshift); ctctggattcaGccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgtgttaatggtgatgggggtaggacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgtgcaagagataattacggtgactactggggccagggaacccctggtcacagtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 93 100 [274, 275] g153>t|a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g153>t| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 225 212 13 5 8 4 2 0 0 0 0 0 1 3 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 30 24 20 4 0 4 3 0 0 0 0 0 0 0 0 1 0 0 117 114 106 8 4 4 1 2 0 0 0 0 0 1 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgt gttaatggtgatgggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgt gttaatggtgatgggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac IGA2 13 213 6 7 2 8 4 5 5 0 4 4 JY8QFUQ01A1UND 1490 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 873 89.4 194/217 nt 88.02 191/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [2.16.38.11] CVRAGFGELSGFDCW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'C' (1 nucleotides at position 15 to 15 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 40 of V-REGION: 2 nucleotides (from position 36 in the user submitted sequence), (cause frameshift)in FR2-IMGT, from codon 45 of V-REGION: 1 nucleotides (from position 48 in the user submitted sequence), (cause frameshift) gcctcctggattcaCcctttggttattatggcatgactggtccgccaactccgggggagggggctgagtgggtctctggtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [289] a72>g|g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>P(+ - -)|a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|t121>c,W41>L(+ - -)|g122>t,W41>L(+ - -)|a138>g|a142>g,K48>E(+ - -)|c148>g,L50>A(+ - +)|t149>c,L50>A(+ - +)|g150>t,L50>A(+ - +)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a72>g|g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>P(+ - -)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| t121>c,W41>L(+ - -)|g122>t,W41>L(+ - -)|a138>g|a142>g,K48>E(+ - -)|c148>g,L50>A(+ - +)|t149>c,L50>A(+ - +)|g150>t,L50>A(+ - +)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 224 (225) 200 24 (25) 4 (5) 20 4 3 2 4 1 (2) 0 2 0 3 2 3 0 78 7 3 4 1 3 1 0 1 1 0 0 0 0 1 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 48 41 7 1 6 2 0 0 2 0 0 0 0 0 1 2 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| gcctcct ggattcacctttggttattatggc atgactggtccgccaactccgggggagggggctgagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgactggtccgccaactccgggggagggggctgagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 19 210 9 10 3 10 6 9 3 0 12 4 JY8QFUQ01A1UXL 10503 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 896 90.37 197/218 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD4-17*01 F 2 8 8 20 8.8.20 [1.17.38.11] CTRTQFDPDYGDYETGYGMDVW in-frame + cggctggattcaacttccgatcttatgccatgtactgggtccgccaggccccaggcaaggggctggactgggtggcagttatttggcatgatggcagtaatcaatactatgcagattccgtgaagggccgattcaccatctccagagacaattccaagaacacattgtttctgcaaatgaacagcctgagagtcgaggacacggctgtctattactgtacgaggacgcaatttgaccctgactacggtgactacgagacggggtacggtatggacgtctggggccaagggacaacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [309] t76>g,S26>A(- + -)|c86>a,T29>N(- + -)|a103>c,S35>R(- - -)|t105>a,S35>R(- - -)|a106>t|g107>c|c108>t|g113>c,G38>A(- + -)|c118>t,H40>Y(+ - -)|t135>c|g153>c,E51>D(+ - +)|a168>t|t172>c,Y58>H(+ - -)|a186>c|a193>c,K65>Q(+ - -)|c207>t|g258>a|c259>t|a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|g303>c|g313>a,A105>T(- - -)| t76>g,S26>A(- + -)| c86>a,T29>N(- + -)|a103>c,S35>R(- - -)|t105>a,S35>R(- - -)|a106>t|g107>c|c108>t|g113>c,G38>A(- + -)| c118>t,H40>Y(+ - -)|t135>c|g153>c,E51>D(+ - +)| a168>t|t172>c,Y58>H(+ - -)|a186>c|a193>c,K65>Q(+ - -)| c207>t|g258>a|c259>t|a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|g303>c| g313>a,A105>T(- - -)|a318>g|g319>a|a320>c| 317 (320) 223 (226) 201 22 (25) 10 (13) 12 0 (1) 2 (3) 5 2 3 (4) 1 3 1 4 0 0 1 78 5 4 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 36 24 17 7 3 4 0 0 1 0 1 1 1 1 2 0 0 0 51 51 48 3 1 2 0 0 1 1 0 0 0 0 1 0 0 0 30 24 20 4 2 2 0 0 0 1 2 0 1 0 0 0 0 0 117 114 108 6 4 2 0 1 3 0 0 0 1 0 1 0 0 0 5 (8) 5 4 1 (4) 0 (3) 1 0 (1) 1 (2) 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cggct ggattcaacttccgatcttatgcc atgtactgggtccgccaggccccaggcaaggggctggactgggtggcagtt atttggcatgatggcagtaatcaa tactatgcagattccgtgaagggccgattcaccatctccagagacaattccaagaacacattgtttctgcaaatgaacagcctgagagtcgaggacacggctgtctattactgt acgaggacgcaatttgaccctgactacggtgactacgagacggggtacggtatggacgtc TRTQFDPDYGDYETGYGMDV IGHV3-33 IGHD4-17 IGHJ6 ggattcaacttccgatcttatgcc atgtactgggtccgccaggccccaggcaaggggctggactgggtggcagtt atttggcatgatggcagtaatcaa tactatgcagattccgtgaagggccgattcaccatctccagagacaattccaagaacacattgtttctgcaaatgaacagcctgagagtcgaggacacggctgtctattactgt acgaggacgcaatttgaccctgactacggtgactacgagacggggtacggtatggacgtc IGA2 20 213 8 12 6 11 2 9 5 5 4 6 JY8QFUQ01A1UXZ 7030 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F (see comment) 750 83.64 179/214 nt NA Homsap IGHJ3*01 F 205 90 45/50 nt Homsap IGHD6-25*01 F 1 8 7 15 8.7.15 [1.17.38.11] CARGTRYNNGLDAFDVW in-frame + Low V-REGION identity (83,64% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctcgagtgcctccatgatcagttactattggacctggattcggcagcccccagggaagggactggagtggattggggacatctattcctttggaggcaccagatacaacccgtcccttggcagtcgagtctccatatcactggacacgtccaataatgagttctccctgcaactgaactctgtgaccgctgcggacacggccttatatcactgtgcgagggggacccggtataacaacggtcttgacgcttttgatgtctggggcccagggacagtggtcatcgtctcttcatcctccaccaagggcccatcggtcttccccctggcaccccctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgcaaccggtgacggtgtcgtggaactc NA IGG1 79 88 [291, 290] t78>g|g79>a,G27>S(+ + -)|g83>c,G28>A(- + -)|c90>g,I30>M(+ + -)|g104>t,S35>I(- - -)|t105>c,S35>I(- - -)|c114>t|g119>c,S40>T(+ - +)|c126>t|t163>g,Y55>D(- - -)|t165>c,Y55>D(- - -)|a173>c,Y58>S(+ - -)|a175>t,S59>F(- - -)|g176>t,S59>F(- - -)|g189>a|a190>g,S64>G(+ + -)|a197>g,N66>R(+ - -)|c198>a,N66>R(+ - -)|c207>g|c213>t|a214>g,K72>G(- - -)|a215>g,K72>G(- - -)|g216>c,K72>G(- - -)|a229>t,T77>S(+ - +)|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|g252>t,K84>N(+ - -)|c255>t|c256>g,Q86>E(+ + -)|a268>c,K90>Q(+ - -)|g270>a,K90>Q(+ - -)|g275>a,S92>N(- - -)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|t307>c,Y103>H(+ - -)| t78>g| g79>a,G27>S(+ + -)|g83>c,G28>A(- + -)|c90>g,I30>M(+ + -)|g104>t,S35>I(- - -)|t105>c,S35>I(- - -)|c114>t| g119>c,S40>T(+ - +)|c126>t|t163>g,Y55>D(- - -)|t165>c,Y55>D(- - -)| a173>c,Y58>S(+ - -)|a175>t,S59>F(- - -)|g176>t,S59>F(- - -)|g189>a|a190>g,S64>G(+ + -)| a197>g,N66>R(+ - -)|c198>a,N66>R(+ - -)|c207>g|c213>t|a214>g,K72>G(- - -)|a215>g,K72>G(- - -)|g216>c,K72>G(- - -)|a229>t,T77>S(+ - +)|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|g252>t,K84>N(+ - -)|c255>t|c256>g,Q86>E(+ + -)|a268>c,K90>Q(+ - -)|g270>a,K90>Q(+ - -)|g275>a,S92>N(- - -)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|t307>c,Y103>H(+ - -)| a318>g|a320>g| 317 (320) 219 (222) 184 (185) 35 (37) 7 (9) 28 5 (7) 5 4 3 2 1 2 0 4 3 4 2 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 36 24 18 6 1 5 0 1 1 1 0 0 0 0 1 1 1 0 51 51 47 4 1 3 0 0 1 1 0 0 0 0 1 0 0 1 30 21 16 5 1 4 1 1 0 0 1 0 1 0 0 0 1 0 117 114 95 19 3 16 4 3 2 1 1 1 1 0 2 2 2 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctcg agtgcctccatgatcagttactat tggacctggattcggcagcccccagggaagggactggagtggattggggac atctattcctttggaggcacc agatacaacccgtcccttggcagtcgagtctccatatcactggacacgtccaataatgagttctccctgcaactgaactctgtgaccgctgcggacacggccttatatcactgt gcgagggggacccggtataacaacggtcttgacgcttttgatgtc ARGTRYNNGLDAFDV IGHV4-59 IGHD6-25 IGHJ3 agtgcctccatgatcagttactat tggacctggattcggcagcccccagggaagggactggagtggattggggac atctattcctttggaggcacc agatacaacccgtcccttggcagtcgagtctccatatcactggacacgtccaataatgagttctccctgcaactgaactctgtgaccgctgcggacacggccttatatcactgt gcgagggggacccggtataacaacggtcttgacgcttttgatgtc IGG1 34 210 17 17 9 21 8 13 4 2 19 9 JY8QFUQ01A1W6G 14350 productive Homsap IGHV4-61*02 F 965 93.21 206/221 nt NA Homsap IGHJ6*02 F 247 88.71 55/62 nt Homsap IGHD3-16*02 F 1 10 7 17 10.7.17 [1.17.38.11] CARGKWGDKPPFYYGMDVW in-frame + tctctggtggctccatcaggagtggtagttactactggagctggatccggcagcccgccgggaagggactggagtggattgggcgtatatatagcagtgggagcatcgacgccaacccctccctcaagagccgagtcaccatatcaattgacacgtccaagaaccaggtctccctgaaactgggctctgtgaccgccgcagacacggccgtctattattgtgcgagagggaagtggggggacaaaccgcccttctactacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [304] c93>g,S31>R(- - -)|c168>a|c173>g,T58>S(+ - +)|c194>t,T65>I(- - -)|a196>g,N66>D(+ + -)|t199>g,Y67>A(- - -)|a200>c,Y67>A(- - -)|t222>c|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|t259>g,F87>V(+ - -)|g270>a|a274>g,S92>G(+ + -)|g303>c|c309>t| c93>g,S31>R(- - -)| c168>a|c173>g,T58>S(+ - +)|c194>t,T65>I(- - -)| a196>g,N66>D(+ + -)|t199>g,Y67>A(- - -)|a200>c,Y67>A(- - -)|t222>c|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|t259>g,F87>V(+ - -)|g270>a|a274>g,S92>G(+ + -)|g303>c|c309>t| a320>g| 319 (320) 228 (229) 213 15 (16) 5 (6) 10 2 (3) 2 2 1 1 1 1 0 1 2 0 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 29 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 18 3 1 2 0 0 1 0 0 1 0 0 0 1 0 0 117 114 103 11 4 7 2 2 1 1 1 0 1 0 1 0 0 2 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcaggagtggtagttactac tggagctggatccggcagcccgccgggaagggactggagtggattgggcgt atatatagcagtgggagcatc gacgccaacccctccctcaagagccgagtcaccatatcaattgacacgtccaagaaccaggtctccctgaaactgggctctgtgaccgccgcagacacggccgtctattattgt gcgagagggaagtggggggacaaaccgcccttctactacggtatggacgtc ARGKWGDKPPFYYGMDV IGHV4-61 IGHD3-16 IGHJ6 ggtggctccatcaggagtggtagttactac tggagctggatccggcagcccgccgggaagggactggagtggattgggcgt atatatagcagtgggagcatc gacgccaacccctccctcaagagccgagtcaccatatcaattgacacgtccaagaaccaggtctccctgaaactgggctctgtgaccgccgcagacacggccgtctattattgt gcgagagggaagtggggggacaaaccgcccttctactacggtatggacgtc IGG1 15 216 7 8 4 8 3 7 4 1 7 3 JY8QFUQ01A1WCP 11576 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 892 89.95 197/219 nt 89.04 195/219 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-10*01 F 3 8 8 10 8.8.10 [2.17.38.11] CSKDTFGREDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'A' (1 nucleotides at position 23 to 23 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 47 of V-REGION: 'G' (1 nucleotides at position 57 to 57 in the user submitted sequence), (cause frameshift); gcctctggattctccgtcagtaAgttactggatgcactgggtccgccaggctccagGgggaggggctggagtgggtctcacgtattaatgaagatggtagtcggacagactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgtagtaaagacacatttgggcgagaagactactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [282] a85>t,T29>S(+ - +)|t88>g,F30>V(+ - -)|c108>t|a132>g|a142>g,K48>E(+ - -)|t152>a,V51>E(- + -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>t|a190>c,S64>R(- - -)|c192>g,S64>R(- - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c234>t|c236>a,S79>Y(+ - -)|a245>g,N82>S(- - -)|g258>a|c287>t,A96>V(+ - +)|g291>a|g303>c|c309>t| a85>t,T29>S(+ - +)|t88>g,F30>V(+ - -)|c108>t| a132>g|a142>g,K48>E(+ - -)|t152>a,V51>E(- + -)| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>t|a190>c,S64>R(- - -)|c192>g,S64>R(- - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c234>t|c236>a,S79>Y(+ - -)|a245>g,N82>S(- - -)|g258>a|c287>t,A96>V(+ - +)|g291>a|g303>c|c309>t| g313>a,A105>S(- + -)|c314>g,A105>S(- + -)|a315>t,A105>S(- + -)|g317>a,R106>K(+ + +)| 320 227 201 26 8 18 5 6 4 0 1 1 2 2 1 2 1 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 1 0 0 0 1 0 0 0 0 1 51 51 48 3 1 2 2 0 0 0 0 0 0 1 0 0 0 0 30 24 18 6 1 5 1 1 0 0 1 0 0 1 0 1 1 0 117 114 104 10 5 5 2 3 3 0 0 1 0 0 1 0 0 0 8 8 4 4 0 4 0 2 0 0 0 0 1 0 0 1 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattctccgtcagtagttactgg atgcactgggtccgccaggctccaggggaggggctggagtgggtctcacgt attaatgaagatggtagtcggaca gactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgt agtaaagacacatttgggcgagaagactac SKDTFGREDY IGHV3-74 IGHD3-10 IGHJ4 ggattctccgtcagtagttactgg atgcactgggtccgccaggctccaggggaggggctggagtgggtctcacgt attaatgaagatggtagtcggaca gactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgt agtaaagacacatttgggcgagaagactac IGA2 22 213 13 9 8 12 5 10 6 2 7 7 JY8QFUQ01A1X35 10873 productive Homsap IGHV4-31*02 F, or Homsap IGHV4-31*03 F or Homsap IGHV4-31*04 F 911 90.5 200/221 nt NA Homsap IGHJ4*02 F (see comment) 231 97.92 47/48 nt Homsap IGHD2-15*01 F 2 10 7 20 10.7.20 [1.17.38.11] CARDRLGSCSGASCPVYYFDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) tctctggtggctccatcaacagtggtgattcctcctggacctggatccgccagtacccagggaagggcctggagtggattgggtacatctctggcagtggggactcctactccaacccggccctcaagagtcgagttaccatatcagtggacacgtctaagagccagttcttcctggaactgagttctgtgactgccgcggacacggccgtctattactgtgcgagagatcggctgggatcttgtagtggggctagctgccccgtctactactttgactcctggggccagggaaccctggtcaccgtctcctcagcatcccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccccg NA IGA1 100 95 [313] g92>a,S31>N(- - -)|g107>a,G36>D(- - -)|a110>c,Y37>S(+ - -)|a113>c,Y38>S(+ - -)|g119>c,S40>T(+ - +)|c133>t,H45>Y(+ - -)|a170>c,Y57>S(+ - -)|t172>g,Y58>G(+ - -)|a173>g,Y58>G(+ - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a193>t,T65>S(+ - +)|a200>c,Y67>S(+ - -)|t208>g,S70>A(- + -)|a240>g|a254>g,N85>S(- - -)|c263>t,S88>F(- - -)|a268>g,K90>E(+ - -)|g270>a,K90>E(+ - -)|c276>t|g303>c| g92>a,S31>N(- - -)|g107>a,G36>D(- - -)|a110>c,Y37>S(+ - -)|a113>c,Y38>S(+ - -)| g119>c,S40>T(+ - +)|c133>t,H45>Y(+ - -)| a170>c,Y57>S(+ - -)|t172>g,Y58>G(+ - -)|a173>g,Y58>G(+ - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a193>t,T65>S(+ - +)| a200>c,Y67>S(+ - -)|t208>g,S70>A(- + -)|a240>g|a254>g,N85>S(- - -)|c263>t,S88>F(- - -)|a268>g,K90>E(+ - -)|g270>a,K90>E(+ - -)|c276>t|g303>c| 320 229 208 21 3 18 5 4 3 0 4 0 1 0 2 0 0 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 0 4 0 2 0 0 2 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 1 0 0 0 0 0 1 0 0 0 30 21 15 6 0 6 2 1 0 0 1 0 1 0 0 0 0 1 117 114 105 9 3 6 3 1 2 0 1 0 0 0 1 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,228-229(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,69-70(FR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,227-228(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|agca,131-134(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agtt,225-228(FR3)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,69-72(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|tacc,228-231(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tgcc,285-288(FR3)|tact,307-310(FR3)| tctct ggtggctccatcaacagtggtgattcctcc tggacctggatccgccagtacccagggaagggcctggagtggattgggtac atctctggcagtggggactcc tactccaacccggccctcaagagtcgagttaccatatcagtggacacgtctaagagccagttcttcctggaactgagttctgtgactgccgcggacacggccgtctattactgt gcgagagatcggctgggatcttgtagtggggctagctgccccgtctactactttgactcc ARDRLGSCSGASCPVYYFDS IGHV4-31 IGHD2-15 IGHJ4 ggtggctccatcaacagtggtgattcctcc tggacctggatccgccagtacccagggaagggcctggagtggattgggtac atctctggcagtggggactcc tactccaacccggccctcaagagtcgagttaccatatcagtggacacgtctaagagccagttcttcctggaactgagttctgtgactgccgcggacacggccgtctattactgt gcgagagatcggctgggatcttgtagtggggctagctgccccgtctactactttgactcc IGA1 21 216 12 9 7 9 5 12 3 0 8 10 JY8QFUQ01A1X52 6383 productive Homsap IGHV3-48*02 F 995 95.41 208/218 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 2 8 8 15 8.8.15 [1.17.38.11] CARVENYSDSGGSFDYW in-frame - cctctggattcaccttcagtagctatagcatgaattgggtccgccaggctccagggaagggactggagtgggtttcatacattagtggaagtagtaataccatgtactacgcagactctgtgaagggccgattcaccatctccagagacaatgcccagaattcactacatctgcaaatgaacagcctgagagacgaggacacggctgtgtattactgtgcgagagtggaaaattactctgatagtggtggttcgtttgactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggttttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcc NA IGA1 100 89 [294] c120>t|g147>a|a172>g,S58>G(+ + -)|t174>a,S58>G(+ + -)|g188>a,S63>N(- - -)|a195>g,I65>M(+ + -)|a250>c,K84>Q(+ - -)|c255>t|g261>a|t262>c,Y88>H(+ - -)| c120>t|g147>a| a172>g,S58>G(+ + -)|t174>a,S58>G(+ + -)|g188>a,S63>N(- - -)|a195>g,I65>M(+ + -)| a250>c,K84>Q(+ - -)|c255>t|g261>a|t262>c,Y88>H(+ - -)| a320>t| 319 (320) 225 (226) 215 10 (11) 4 (5) 6 2 3 2 1 1 0 0 (1) 1 0 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 2 0 0 1 1 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 2 1 0 0 0 0 0 1 0 0 0 0 117 114 110 4 2 2 0 1 1 1 1 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtagctatagc atgaattgggtccgccaggctccagggaagggactggagtgggtttcatac attagtggaagtagtaataccatg tactacgcagactctgtgaagggccgattcaccatctccagagacaatgcccagaattcactacatctgcaaatgaacagcctgagagacgaggacacggctgtgtattactgt gcgagagtggaaaattactctgatagtggtggttcgtttgactat ARVENYSDSGGSFDY IGHV3-48 IGHD3-22 IGHJ4 ggattcaccttcagtagctatagc atgaattgggtccgccaggctccagggaagggactggagtgggtttcatac attagtggaagtagtaataccatg tactacgcagactctgtgaagggccgattcaccatctccagagacaatgcccagaattcactacatctgcaaatgaacagcctgagagacgaggacacggctgtgtattactgt gcgagagtggaaaattactctgatagtggtggttcgtttgactat IGA1 10 213 8 2 5 5 3 5 4 0 2 4 JY8QFUQ01A1Y2H 11083 productive Homsap IGHV3-48*02 F, or Homsap IGHV3-48*04 F 921 92.52 198/214 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-21*02 F 2 8 8 11 8.8.11 [0.17.38.11] CAKVGNLYFFEYW in-frame - tggattcagcttcagtagctacagtatgaactgggtccgccaggctccagggaaggggctggagtggatttcatctattagcaccaccagtagtaccataggctacgcagactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtttctgcaaatgaacagcctgagagacgaggacacggctgtgtactactgtgccaaagtgggtaacctgtatttctttgaatactggggccggggaaccctggtcaccgtctccccagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [278] c86>g,T29>S(+ - +)|t111>c|c114>t|g157>a,V53>I(+ - +)|a164>c,Y55>S(+ - -)|c165>t,Y55>S(+ - -)|t171>c|g173>c,S58>T(+ - +)|t174>c,S58>T(+ - +)|g176>c,S59>T(+ - +)|t177>c,S59>T(+ - +)|t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|t246>c|a263>t,Y88>F(- + -)|t306>c|g315>c|g317>a,R106>K(+ + +)| c86>g,T29>S(+ - +)|t111>c|c114>t| g157>a,V53>I(+ - +)|a164>c,Y55>S(+ - -)|c165>t,Y55>S(+ - -)| t171>c|g173>c,S58>T(+ - +)|t174>c,S58>T(+ - +)|g176>c,S59>T(+ - +)|t177>c,S59>T(+ - +)| t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|t246>c|a263>t,Y88>F(- + -)|t306>c| g315>c|g317>a,R106>K(+ + +)|a320>t| 319 (320) 221 (222) 203 18 (19) 6 (7) 12 1 2 2 6 1 0 1 (2) 0 3 1 0 1 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 1 1 0 0 0 0 0 1 0 0 51 51 48 3 0 3 0 1 1 0 1 0 0 0 0 0 0 0 30 24 19 5 1 4 0 0 0 3 0 0 0 0 2 0 0 0 117 114 109 5 2 3 1 0 0 2 0 0 1 0 0 0 0 1 7 (8) 7 5 2 (3) 1 (2) 1 0 1 0 0 0 0 0 (1) 0 1 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggattcagcttcagtagctacagt atgaactgggtccgccaggctccagggaaggggctggagtggatttcatct attagcaccaccagtagtaccata ggctacgcagactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtttctgcaaatgaacagcctgagagacgaggacacggctgtgtactactgt gccaaagtgggtaacctgtatttctttgaatac AKVGNLYFFEY IGHV3-48 IGHD2-21 IGHJ4 ggattcagcttcagtagctacagt atgaactgggtccgccaggctccagggaaggggctggagtggatttcatct attagcaccaccagtagtaccata ggctacgcagactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtttctgcaaatgaacagcctgagagacgaggacacggctgtgtactactgt gccaaagtgggtaacctgtatttctttgaatac IGA1 16 213 10 6 3 6 7 10 2 3 6 5 JY8QFUQ01A1YN6 15265 productive Homsap IGHV3-48*02 F 1026 97.24 211/217 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD1-1*01 F 2 8 8 20 8.8.20 [1.17.38.11] CASGERKDYEVRRDYYHGMDVW in-frame - ctctggactcaccttcagcagctatagtatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatatattagtagtactagtagtaccataaactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagacgaggacacggctgtgtattactgtgcgagtggggaaaggaaagactacgaagtacgacgggactactatcacggcatggacgtctggggccaagggactacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [308] t82>c,F28>L(+ - -)|t105>c|c114>t|c165>t|g176>c,S59>T(+ - +)|t196>a,Y66>N(- - -)| t82>c,F28>L(+ - -)|t105>c|c114>t| c165>t| g176>c,S59>T(+ - +)| t196>a,Y66>N(- - -)| a318>t,R106>S(- - -)|a320>g| 317 (320) 222 (225) 216 (217) 6 (8) 3 (4) 3 (4) 0 (1) 0 2 2 0 0 0 (1) 1 1 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 1 2 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 117 114 113 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (1) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ctct ggactcaccttcagcagctatagt atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatat attagtagtactagtagtaccata aactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagacgaggacacggctgtgtattactgt gcgagtggggaaaggaaagactacgaagtacgacgggactactatcacggcatggacgtc ASGERKDYEVRRDYYHGMDV IGHV3-48 IGHD1-1 IGHJ6 ggactcaccttcagcagctatagt atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatat attagtagtactagtagtaccata aactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagacgaggacacggctgtgtattactgt gcgagtggggaaaggaaagactacgaagtacgacgggactactatcacggcatggacgtc IGA1 6 213 4 2 2 3 2 3 1 2 1 2 JY8QFUQ01A1Z5H 5788 productive Homsap IGHV3-64*02 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 2 8 8 10 8.8.10 [1.17.38.11] CVTCGDYSYEYW in-frame + ctctggattcattttcagtacttatcctatgcactgggtccgccaggctccagggaagggactggaatatgtttcagctattagtcgtaatggggataacgcatattatgcagactctgtgaagggcagattcaccatgtccagagacaattccaagagcacactgtatcttcagatgggcagcctgagagctgaggacatggctgtgtattactgtgtgacatgtggtgattacagctatgagtactggggccagggaaccttggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 89 [278, 277] c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>c,A38>P(- - -)|a172>c,S58>R(- - -)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|a193>g,T65>A(- - -)|c234>g,I78>M(+ + -)|a254>g,N85>S(- - -)|g258>a|a270>g|c314>t,A105>V(+ - +)| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>c,A38>P(- - -)| a172>c,S58>R(- - -)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|a193>g,T65>A(- - -)| c234>g,I78>M(+ + -)|a254>g,N85>S(- - -)|g258>a|a270>g| c314>t,A105>V(+ - +)|g317>c,R106>T(- - -)|g319>t|a320>g| 316 (320) 221 (225) 207 (208) 14 (17) 2 (4) 12 (13) 3 (4) 3 4 0 1 0 0 0 2 (3) 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 0 3 0 0 0 0 0 2 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 2 0 0 1 0 0 0 0 0 0 0 117 114 110 4 2 2 2 1 0 0 0 0 0 0 0 1 0 0 4 (8) 4 3 (4) 1 (4) 0 (2) 1 (2) 0 (1) 0 1 0 0 0 0 0 0 (1) 0 0 (1) 0 aa,26-27(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|aa,152-153(FR2)|ta,154-155(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,154-155(FR2)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|tt,198-199(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|agct,162-165(FR2)|agta,169-172(CDR2)|ggta,187-190(CDR2)|ggca,220-223(FR3)|ggca,274-277(FR3)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|agct,162-165(FR2)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcattttcagtacttatcct atgcactgggtccgccaggctccagggaagggactggaatatgtttcagct attagtcgtaatggggataacgca tattatgcagactctgtgaagggcagattcaccatgtccagagacaattccaagagcacactgtatcttcagatgggcagcctgagagctgaggacatggctgtgtattactgt gtgacatgtggtgattacagctatgagtac VTCGDYSYEY IGHV3-64 IGHD3-22 IGHJ4 ggattcattttcagtacttatcct atgcactgggtccgccaggctccagggaagggactggaatatgtttcagct attagtcgtaatggggataacgca tattatgcagactctgtgaagggcagattcaccatgtccagagacaattccaagagcacactgtatcttcagatgggcagcctgagagctgaggacatggctgtgtattactgt gtgacatgtggtgattacagctatgagtac IGA2 13 213 9 4 6 9 3 4 2 0 2 9 JY8QFUQ01A23OB 20172 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 941 92.66 202/218 nt NA Homsap IGHJ4*02 F 69 60.42 29/48 nt Homsap IGHD4-17*01 F 2 8 8 12 8.8.12 [1.17.38.11] CARFGPLTRPDYWG in-frame + cctctggattcacctttaccaactatggcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtggtgaaaccacataccacgcagaatccgtgcagggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgtgcgagattcggaccacttactcgacctgactactgggggccaggggaacccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [286] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)|c164>g,A55>G(- + -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>c,S64>T(+ - +)|t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a214>c,K72>Q(+ - -)|t246>c|c257>a,T86>N(- + -)|g258>t,T86>N(- + -)|c279>t|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)| c164>g,A55>G(- + -)| g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>c,S64>T(+ - +)| t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a214>c,K72>Q(+ - -)|t246>c|c257>a,T86>N(- + -)|g258>t,T86>N(- + -)|c279>t|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| a317>g,K106>R(+ + +)|g319>t|a320>t| 316 (320) 222 (226) 206 (207) 16 (19) 2 (4) 14 (15) 0 (1) 3 1 2 1 2 1 (2) 1 2 2 1 (2) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 21 3 0 3 0 1 0 0 0 0 0 1 1 0 0 0 117 114 105 9 2 7 0 1 1 2 1 2 1 0 0 0 1 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgaaaccaca taccacgcagaatccgtgcagggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgt gcgagattcggaccacttactcgacctgactactgg ARFGPLTRPDYW IGHV3-23 IGHD4-17 IGHJ4 ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgaaaccaca taccacgcagaatccgtgcagggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgt gcgagattcggaccacttactcgacctgactactgg IGG2 16 213 6 10 4 11 2 5 2 0 8 6 JY8QFUQ01A23UZ 13462 productive Homsap IGHV4-4*07 F 818 86.98 187/215 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD6-25*01 F 1 8 7 15 8.7.15 [1.17.38.11] CARQRGGGDRQWAFENW in-frame + tctctggtacctccatcagcacttactattggagttggttccggcagcccgccgagaagggactggagtggattgggcgtatctctgtctttgaaaactctaactacaacccctccctcgagagtcgcatcaccatgtcaatggacacgtccaagaaccagttctccctgacggtgaactctgtgaccgccgcggacacggccgtgtatttttgtgcgagacaaagaggcggcggcgaccgacagtgggcctttgagaactggggccagggaacccaaatcaccgtctccgcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG2 93 100 [292] g82>a,G28>T(+ - -)|g83>c,G28>T(+ - -)|t105>c|g107>c,S36>T(+ - +)|c114>t|c120>t|a124>t,I42>F(+ - -)|g140>a,G47>E(- - -)|a170>c,Y57>S(+ - -)|a172>g,T58>V(- - -)|c173>t,T58>V(- - -)|a175>t,S59>F(- - -)|g176>t,S59>F(- - -)|g188>a,G63>E(- - -)|g189>a,G63>E(- - -)|g191>a,S64>N(- - -)|a193>t,T65>S(+ - +)|c195>t,T65>S(+ - +)|a214>g,K72>E(+ - -)|a225>c|g226>a,V76>I(+ - +)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|a269>c,K90>T(- - -)|c271>g,L91>V(+ - +)|g275>a,S92>N(- - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g82>a,G28>T(+ - -)|g83>c,G28>T(+ - -)|t105>c|g107>c,S36>T(+ - +)|c114>t| c120>t|a124>t,I42>F(+ - -)|g140>a,G47>E(- - -)| a170>c,Y57>S(+ - -)|a172>g,T58>V(- - -)|c173>t,T58>V(- - -)|a175>t,S59>F(- - -)|g176>t,S59>F(- - -)|g188>a,G63>E(- - -)|g189>a,G63>E(- - -)|g191>a,S64>N(- - -)|a193>t,T65>S(+ - +)|c195>t,T65>S(+ - +)| a214>g,K72>E(+ - -)|a225>c|g226>a,V76>I(+ - +)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|a269>c,K90>T(- - -)|c271>g,L91>V(+ - +)|g275>a,S92>N(- - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g319>c| 318 (320) 221 (223) 193 (194) 28 (29) 4 (5) 24 3 8 5 1 3 0 4 0 2 (3) 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 1 1 1 0 0 0 0 2 0 0 0 51 51 48 3 1 2 0 1 1 0 0 0 1 0 0 0 0 0 30 21 11 10 0 10 1 3 2 0 1 0 2 0 0 0 1 0 117 114 104 10 1 9 2 3 1 0 2 0 1 0 0 1 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtacctccatcagcacttactat tggagttggttccggcagcccgccgagaagggactggagtggattgggcgt atctctgtctttgaaaactct aactacaacccctccctcgagagtcgcatcaccatgtcaatggacacgtccaagaaccagttctccctgacggtgaactctgtgaccgccgcggacacggccgtgtatttttgt gcgagacaaagaggcggcggcgaccgacagtgggcctttgagaac ARQRGGGDRQWAFEN IGHV4-4 IGHD6-25 IGHJ4 ggtacctccatcagcacttactat tggagttggttccggcagcccgccgagaagggactggagtggattgggcgt atctctgtctttgaaaactct aactacaacccctccctcgagagtcgcatcaccatgtcaatggacacgtccaagaaccagttctccctgacggtgaactctgtgaccgccgcggacacggccgtgtatttttgt gcgagacaaagaggcggcggcgaccgacagtgggcctttgagaac IGG2 28 210 17 11 13 17 4 11 2 2 11 13 JY8QFUQ01A26C2 5092 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 963 94.01 204/217 nt NA Homsap IGHJ2*01 F 247 96.23 51/53 nt Homsap IGHD2-21*01 F 1 8 8 12 8.8.12 [1.17.38.11] CAKVVWSLWYFDLW in-frame + ctctacattcgcctttagcaactatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtgtcagatattagtgggagtggtgttagcacacactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgtgcgaaagtagtctggagcctctggtacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [285] g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)|c159>g|c164>a,A55>D(- - -)|t174>g|g188>t,G63>V(- - -)|t196>c,Y66>H(+ - -)|a204>g|a214>c,K72>Q(+ - -)|c249>g|a285>t,R95>S(- - -)| g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)| c159>g|c164>a,A55>D(- - -)| t174>g|g188>t,G63>V(- - -)| t196>c,Y66>H(+ - -)|a204>g|a214>c,K72>Q(+ - -)|c249>g|a285>t,R95>S(- - -)| a320>t| 319 (320) 224 (225) 211 13 (14) 4 (5) 9 2 2 0 1 1 1 1 (2) 0 1 2 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 2 0 0 0 0 0 0 1 0 0 0 51 51 49 2 1 1 0 0 0 0 0 1 0 0 0 1 0 0 30 24 22 2 1 1 0 0 0 0 0 0 0 0 0 0 1 1 117 114 109 5 2 3 1 0 0 1 1 0 1 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct acattcgcctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtgtcagat attagtgggagtggtgttagcaca cactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgt gcgaaagtagtctggagcctctggtacttcgatctc AKVVWSLWYFDL IGHV3-23 IGHD2-21 IGHJ2 acattcgcctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtgtcagat attagtgggagtggtgttagcaca cactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgt gcgaaagtagtctggagcctctggtacttcgatctc IGG1 13 213 5 8 2 7 3 6 3 1 4 5 JY8QFUQ01A26DA 17504 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F or Homsap IGHV3-15*06 F (see comment) 768 82.96 185/223 nt NA Homsap IGHJ5*02 F (see comment) 147 76.47 39/51 nt Homsap IGHD3-22*01 F 2 8 10 13 8.10.13 [1.17.38.11] CSTGGPNGYHSTYPW in-frame + Low V-REGION identity (82,96% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctggattcgccttcaatatcaagtggatgagttgggtccgccaggctccggggaaggggcttgagtgggtcggccgcatcaagagcagcgctgatggtgcgacaacagacaccattgagcgcgtgagagacagattcaccatctcaagagatgactcaaaaaatacactgtacttgcacatgaccagcctgagaaccgaggacacaggcatgtattattgttccacaggcgggccaaatggatatcactccacatatccctggggcccggggacccaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [293] a85>g,T29>A(- - -)|t87>c,T29>A(- - -)|g104>a,S35>N(- - -)|a107>t,N36>I(- - -)|g109>a,A37>K(- - -)|c110>a,A37>K(- - -)|c111>g,A37>K(- - -)|c120>t|a138>g|g150>t|t159>c|t165>c|t168>c|a171>g|a176>g,K59>S(- - -)|a177>c,K59>S(- - -)|a178>g,T60>A(- - -)|g188>c,G63>A(- + -)|t199>a,Y67>T(+ - -)|a200>c,Y67>T(+ - -)|g202>a,A68>I(+ - +)|c203>t,A68>I(+ - +)|c206>a,A69>E(- - -)|a207>g,A69>E(- - -)|c209>g,P70>R(- - -)|a215>g,K72>R(+ + +)|g221>a,G74>D(- - -)|t246>c|c255>t|g258>a|t264>c|c265>t|a270>c,Q90>H(- + -)|a275>c,N92>T(- + -)|a284>g,K95>R(+ + +)|c299>g,A100>G(- + -)|g301>a,V101>M(+ - -)|c309>t|a313>t,T105>S(+ - +)| a85>g,T29>A(- - -)|t87>c,T29>A(- - -)|g104>a,S35>N(- - -)|a107>t,N36>I(- - -)|g109>a,A37>K(- - -)|c110>a,A37>K(- - -)|c111>g,A37>K(- - -)| c120>t|a138>g|g150>t|t159>c|t165>c| t168>c|a171>g|a176>g,K59>S(- - -)|a177>c,K59>S(- - -)|a178>g,T60>A(- - -)|g188>c,G63>A(- + -)| t199>a,Y67>T(+ - -)|a200>c,Y67>T(+ - -)|g202>a,A68>I(+ - +)|c203>t,A68>I(+ - +)|c206>a,A69>E(- - -)|a207>g,A69>E(- - -)|c209>g,P70>R(- - -)|a215>g,K72>R(+ + +)|g221>a,G74>D(- - -)|t246>c|c255>t|g258>a|t264>c|c265>t|a270>c,Q90>H(- + -)|a275>c,N92>T(- + -)|a284>g,K95>R(+ + +)|c299>g,A100>G(- + -)|g301>a,V101>M(+ - -)|c309>t| a313>t,T105>S(+ - +)|a320>g| 319 (320) 230 (231) 191 39 (40) 13 (14) 26 8 (9) 6 5 6 4 2 2 1 1 3 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 0 7 1 2 0 1 0 1 1 0 0 1 0 0 51 51 46 5 5 0 1 0 1 2 0 0 0 0 0 0 1 0 30 30 24 6 2 4 3 0 0 1 1 0 0 0 1 0 0 0 117 114 94 20 6 14 3 4 4 2 3 1 0 1 0 2 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 0 0 0 0 1 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| ctct ggattcgccttcaatatcaagtgg atgagttgggtccgccaggctccggggaaggggcttgagtgggtcggccgc atcaagagcagcgctgatggtgcgacaaca gacaccattgagcgcgtgagagacagattcaccatctcaagagatgactcaaaaaatacactgtacttgcacatgaccagcctgagaaccgaggacacaggcatgtattattgt tccacaggcgggccaaatggatatcactccacatatccc STGGPNGYHSTYP IGHV3-15 IGHD3-22 IGHJ5 ggattcgccttcaatatcaagtgg atgagttgggtccgccaggctccggggaaggggcttgagtgggtcggccgc atcaagagcagcgctgatggtgcgacaaca gacaccattgagcgcgtgagagacagattcaccatctcaagagatgactcaaaaaatacactgtacttgcacatgaccagcctgagaaccgaggacacaggcatgtattattgt tccacaggcgggccaaatggatatcactccacatatccc IGA1 38 219 25 13 11 18 14 20 11 2 14 11 JY8QFUQ01A27H2 7326 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 923 91.74 200/218 nt 91.28 199/218 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-10*01 F 2 8 8 13 8.8.13 [1.17.38.11] CANWLSGTHSKFDYW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 59 of V-REGION: 'A' (1 nucleotides at position 91 to 91 in the user submitted sequence), (cause frameshift); gctctggattcaccttcagcaagtatgccatgagctgggtccgccaggctccaggggaggggctgcagtgggtctcagcaattagtggaaAatggtgctgatatatactacgcagactccgtgaacggccagttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgtgcgaattggctttcggggacacattctaagtttgactactggggcaagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [289] c74>g|t90>c|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a142>g,K48>E(+ - -)|g151>c,E51>Q(+ + -)|t165>a|t174>a|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c192>t,S64>D(- - -)|c194>t,T65>I(- - -)|g216>c,K72>N(+ - -)|g224>a,R75>Q(+ - -)|g261>a|t306>c| c74>g| t90>c|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)| a142>g,K48>E(+ - -)|g151>c,E51>Q(+ + -)|t165>a| t174>a|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c192>t,S64>D(- - -)|c194>t,T65>I(- - -)| g216>c,K72>N(+ - -)|g224>a,R75>Q(+ - -)|g261>a|t306>c| a318>t,K106>N(+ - -)|g319>t|a320>g| 317 (320) 223 (226) 205 18 (21) 6 (8) 12 (13) 2 (3) 5 2 2 0 0 0 (1) 2 3 2 0 (1) 0 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 21 3 1 2 0 1 0 1 0 0 0 0 0 1 0 0 51 51 48 3 1 2 1 0 0 0 0 0 0 1 1 0 0 0 30 24 17 7 1 6 1 2 2 0 0 0 0 1 1 0 0 0 117 114 110 4 2 2 0 2 0 1 0 0 0 0 1 0 0 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gctct ggattcaccttcagcaagtatgcc atgagctgggtccgccaggctccaggggaggggctgcagtgggtctcagca attagtggaaatggtgctgatata tactacgcagactccgtgaacggccagttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgt gcgaattggctttcggggacacattctaagtttgactac ANWLSGTHSKFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcaccttcagcaagtatgcc atgagctgggtccgccaggctccaggggaggggctgcagtgggtctcagca attagtggaaatggtgctgatata tactacgcagactccgtgaacggccagttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgt gcgaattggctttcggggacacattctaagtttgactac IGA2 17 213 11 6 7 11 4 6 3 2 4 8 JY8QFUQ01A27QT 4768 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 809 86.51 186/215 nt NA Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD6-6*01 F 1 8 7 13 8.7.13 [1.17.38.11] CARDSSNWSDAFDIW in-frame - tctctggtggctccatgagtagtcactattggagttggatccggcaatcttcagggaagggactggagtggcttggctacatacattacagtgggagcaccaggttcaacccctccctcaacagtcgagtcaccatatcggtggacacgtccaagagtcagttcttcctgctactgaactctgtgaccgctgcggacacggccacatattactgtgcgagagactctagcaattggtcagacgcttttgatatctggagccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [285] c90>g,I30>M(+ + -)|t109>c,Y37>H(+ - -)|c114>t|c120>t|g132>a|c133>t,P45>S(+ - -)|c135>t,P45>S(+ - -)|c136>t,P46>S(+ - -)|a157>c,I53>L(+ + +)|g162>c|t165>c|c168>a|t169>c,Y57>H(+ - -)|a197>g,N66>R(+ - -)|c198>g,N66>R(+ - -)|a200>t,Y67>F(- + -)|g216>c,K72>N(+ - -)|a237>g|a240>g|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c263>t,S88>F(- - -)|a268>c,K90>L(- + -)|a269>t,K90>L(- + -)|g270>a,K90>L(- + -)|g275>a,S92>N(- - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)| c90>g,I30>M(+ + -)|t109>c,Y37>H(+ - -)|c114>t| c120>t|g132>a|c133>t,P45>S(+ - -)|c135>t,P45>S(+ - -)|c136>t,P46>S(+ - -)|a157>c,I53>L(+ + +)|g162>c|t165>c| c168>a|t169>c,Y57>H(+ - -)| a197>g,N66>R(+ - -)|c198>g,N66>R(+ - -)|a200>t,Y67>F(- + -)|g216>c,K72>N(+ - -)|a237>g|a240>g|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c263>t,S88>F(- - -)|a268>c,K90>L(- + -)|a269>t,K90>L(- + -)|g270>a,K90>L(- + -)|g275>a,S92>N(- - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)| 320 223 194 29 8 21 4 5 7 4 2 1 2 0 2 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 1 1 0 0 0 0 0 1 0 0 51 51 43 8 4 4 0 1 4 1 1 0 0 0 1 0 0 0 30 21 19 2 1 1 0 0 0 1 0 1 0 0 0 0 0 0 117 114 98 16 2 14 4 4 2 1 1 0 2 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatgagtagtcactat tggagttggatccggcaatcttcagggaagggactggagtggcttggctac atacattacagtgggagcacc aggttcaacccctccctcaacagtcgagtcaccatatcggtggacacgtccaagagtcagttcttcctgctactgaactctgtgaccgctgcggacacggccacatattactgt gcgagagactctagcaattggtcagacgcttttgatatc ARDSSNWSDAFDI IGHV4-59 IGHD6-6 IGHJ3 ggtggctccatgagtagtcactat tggagttggatccggcaatcttcagggaagggactggagtggcttggctac atacattacagtgggagcacc aggttcaacccctccctcaacagtcgagtcaccatatcggtggacacgtccaagagtcagttcttcctgctactgaactctgtgaccgctgcggacacggccacatattactgt gcgagagactctagcaattggtcagacgcttttgatatc IGA1 29 210 20 9 12 17 8 12 6 2 18 3 JY8QFUQ01A287O 10350 productive Homsap IGHV3-13*01 F 976 94.91 205/216 nt NA Homsap IGHJ5*02 F 255 100 51/51 nt Homsap IGHD3-9*01 F 1 8 7 13 8.7.13 [2.17.38.11] CARGSSKAYNWFDPW in-frame - gcctctggattcacgttcagtaattacgacatgcactgggtccgccaacctagaggaagaggtctggagtgggtctcagctattggcactggtggtgacacatactatccagactccgtgaagggccgattcaccatctccagagaaaatgccaagaactccttatatcttcagatgaacagcctgagagccggggacacggctgtgtattactgtgcaagagggtcgtcaaaagcgtacaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [287] c87>g|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g133>c,A45>P(- - -)|c137>g,T46>R(- - -)|a143>g,K48>R(+ + +)|t171>c|c176>g,A59>G(- + -)|g206>a,G69>D(- - -)|g261>a|a270>g| c87>g|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g133>c,A45>P(- - -)|c137>g,T46>R(- - -)|a143>g,K48>R(+ + +)| t171>c|c176>g,A59>G(- + -)| g206>a,G69>D(- - -)|g261>a|a270>g| a320>g| 319 (320) 223 (224) 212 11 (12) 4 (5) 7 2 (3) 3 1 1 0 0 0 0 1 3 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 0 0 0 0 0 0 1 0 0 51 51 48 3 0 3 1 0 0 0 0 0 0 0 1 1 0 0 30 21 19 2 1 1 0 0 0 1 0 0 0 0 0 1 0 0 117 114 111 3 2 1 1 2 0 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|ta,135-136(FR2)|aa,141-142(FR2)|aa,143-144(FR2)|ta,165-166(FR2-CDR2)|ta,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,242-243(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,135-136(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,259-260(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|agct,162-165(FR2)|ggta,169-172(CDR2)|ggct,205-208(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,162-165(FR2)|tact,171-174(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacgttcagtaattacgac atgcactgggtccgccaacctagaggaagaggtctggagtgggtctcagct attggcactggtggtgacaca tactatccagactccgtgaagggccgattcaccatctccagagaaaatgccaagaactccttatatcttcagatgaacagcctgagagccggggacacggctgtgtattactgt gcaagagggtcgtcaaaagcgtacaactggttcgacccc ARGSSKAYNWFDP IGHV3-13 IGHD3-9 IGHJ5 ggattcacgttcagtaattacgac atgcactgggtccgccaacctagaggaagaggtctggagtgggtctcagct attggcactggtggtgacaca tactatccagactccgtgaagggccgattcaccatctccagagaaaatgccaagaactccttatatcttcagatgaacagcctgagagccggggacacggctgtgtattactgt gcaagagggtcgtcaaaagcgtacaactggttcgacccc IGG1 11 210 7 4 4 8 3 3 2 2 4 3 JY8QFUQ01A29EP 18991 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 1085 100 218/218 nt 99.54 217/218 nt Homsap IGHJ5*02 F 255 100 51/51 nt Homsap IGHD2-2*01 F 3 8 8 18 8.8.18 [1.17.38.11] CAKFMGVVVVPAAHNWFDPW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 55 of V-REGION: 'G' (1 nucleotides at position 80 to 80 in the user submitted sequence), (cause frameshift); cctctggattcacctttagcagctatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtctcagcGtattagtggtagtggtggtagcacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaaattcatgggagttgtagtagtaccagctgcccacaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG2 100 100 [305] g319>t|a320>t| 318 (320) 224 (226) 224 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagcagctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagct attagtggtagtggtggtagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaattcatgggagttgtagtagtaccagctgcccacaactggttcgacccc AKFMGVVVVPAAHNWFDP IGHV3-23 IGHD2-2 IGHJ5 ggattcacctttagcagctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagct attagtggtagtggtggtagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaattcatgggagttgtagtagtaccagctgcccacaactggttcgacccc IGG2 0 213 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01A2AEH 13881 productive Homsap IGHV3-7*01 F 891 90.32 196/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-12*01 F 3 8 8 14 8.8.14 [1.17.38.11] CARGSSGFNDAYFDNW in-frame + ctccggattcacctttaacacttattggatgaactgggtccgccaggctccagggaagggactggagtgggtggccaacataaaccaagatgggagcaggagacactatgtggactctgtgatgggccgattcaccatctccagagacaacaccaaggactcactgtatctgcaaatggacagcgtgagagccgaagacacggctgtctattactgtgcgaggggatctagtggattcaacgacgcctattttgacaactggggccagggaaccctggccacagtatcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatggaacgtggtcatcgcctgcctggtccagggtcttcttcccccaag NA IGA1 100 89 [290, 289] t78>c|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g119>a,S40>N(- - -)|g147>a|g171>c,K57>N(+ - -)|a186>g|t189>c|g190>a,E64>R(+ - -)|a191>g,E64>R(+ - -)|a194>g,K65>R(+ + +)|t196>c,Y66>H(+ - -)|a215>t,K72>M(- + -)|g247>a,A83>T(- - -)|a253>g,N85>D(+ + -)|a274>g,N92>D(+ + -)|c280>g,L94>V(+ - +)|g291>a|g303>c| t78>c| g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)| g119>a,S40>N(- - -)|g147>a| g171>c,K57>N(+ - -)|a186>g|t189>c|g190>a,E64>R(+ - -)|a191>g,E64>R(+ - -)|a194>g,K65>R(+ + +)| t196>c,Y66>H(+ - -)|a215>t,K72>M(- + -)|g247>a,A83>T(- - -)|a253>g,N85>D(+ + -)|a274>g,N92>D(+ + -)|c280>g,L94>V(+ - +)|g291>a|g303>c| a318>g|a320>g| 317 (320) 222 (225) 201 (202) 21 (23) 6 (8) 15 5 (7) 6 1 4 0 0 1 0 3 1 0 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 1 1 0 0 0 0 1 0 0 0 51 51 49 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 30 24 18 6 2 4 3 1 0 1 0 0 0 0 1 0 0 0 117 114 106 8 2 6 2 2 0 1 0 0 1 0 1 1 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcacctttaacacttattgg atgaactgggtccgccaggctccagggaagggactggagtgggtggccaac ataaaccaagatgggagcaggaga cactatgtggactctgtgatgggccgattcaccatctccagagacaacaccaaggactcactgtatctgcaaatggacagcgtgagagccgaagacacggctgtctattactgt gcgaggggatctagtggattcaacgacgcctattttgacaac ARGSSGFNDAYFDN IGHV3-7 IGHD5-12 IGHJ4 ggattcacctttaacacttattgg atgaactgggtccgccaggctccagggaagggactggagtgggtggccaac ataaaccaagatgggagcaggaga cactatgtggactctgtgatgggccgattcaccatctccagagacaacaccaaggactcactgtatctgcaaatggacagcgtgagagccgaagacacggctgtctattactgt gcgaggggatctagtggattcaacgacgcctattttgacaac IGA1 20 213 15 5 7 11 8 9 3 2 7 8 JY8QFUQ01A2ANY 5859 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 829 86.76 190/219 nt 86.3 189/219 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-15*01 F 2 8 8 19 8.8.19 [2.17.38.11] CAKDYGDCRGGRCYAEPLDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 14 to 14 in the user submitted sequence), (cause frameshift); gcctctggattcaGcctcgccgacttatagtatgagttgggtccgccaggctccaggaaaggggctggagtgggtctcaggtattagtgatcatggtattgacatatactatgcagactccgtgaggggccggtttaccatctccagagacatttccaagaacacggtgtatctacaaatgaacagcctgggagtcgaggacacggccgtatattactgtgcgaaagattacggggactgtcgtggtggtagatgctacgccgagcccctcgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggttcagggcttcttccccca NA IGA1 100 100 [308] t89>c,F30>S(- - -)|t90>g,F30>S(- - -)|a103>c,S35>P(+ - -)|g104>c,S35>P(+ - -)|c105>g,S35>P(+ - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>a,A38>S(- + -)|c113>g,A38>S(- + -)|c114>t,A38>S(- + -)|c120>t|g141>a|c164>g,A55>G(- + -)|g173>a,G58>D(- - -)|a175>c,S59>H(+ - -)|g176>a,S59>H(+ - -)|g187>a,G63>I(- - -)|g188>t,G63>I(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>t,T65>I(- - -)|c201>t|a215>g,K72>R(+ + +)|c228>t|a245>t,N82>I(- - -)|c259>g,L87>V(+ - +)|g267>a|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)| t89>c,F30>S(- - -)|t90>g,F30>S(- - -)|a103>c,S35>P(+ - -)|g104>c,S35>P(+ - -)|c105>g,S35>P(+ - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>a,A38>S(- + -)|c113>g,A38>S(- + -)|c114>t,A38>S(- + -)| c120>t|g141>a|c164>g,A55>G(- + -)| g173>a,G58>D(- - -)|a175>c,S59>H(+ - -)|g176>a,S59>H(+ - -)|g187>a,G63>I(- - -)|g188>t,G63>I(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>t,T65>I(- - -)| c201>t|a215>g,K72>R(+ + +)|c228>t|a245>t,N82>I(- - -)|c259>g,L87>V(+ - +)|g267>a|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)| 320 227 198 29 5 24 3 7 7 1 2 0 1 0 2 4 1 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 14 10 0 10 0 1 2 1 1 0 0 0 2 2 0 1 51 51 48 3 2 1 0 1 1 0 0 0 0 0 0 1 0 0 30 24 16 8 0 8 1 4 1 0 1 0 0 0 0 0 1 0 117 114 106 8 3 5 2 1 3 0 0 0 1 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacctcgccgacttatagt atgagttgggtccgccaggctccaggaaaggggctggagtgggtctcaggt attagtgatcatggtattgacata tactatgcagactccgtgaggggccggtttaccatctccagagacatttccaagaacacggtgtatctacaaatgaacagcctgggagtcgaggacacggccgtatattactgt gcgaaagattacggggactgtcgtggtggtagatgctacgccgagcccctcgactac AKDYGDCRGGRCYAEPLDY IGHV3-23 IGHD2-15 IGHJ4 ggattcacctcgccgacttatagt atgagttgggtccgccaggctccaggaaaggggctggagtgggtctcaggt attagtgatcatggtattgacata tactatgcagactccgtgaggggccggtttaccatctccagagacatttccaagaacacggtgtatctacaaatgaacagcctgggagtcgaggacacggccgtatattactgt gcgaaagattacggggactgtcgtggtggtagatgctacgccgagcccctcgactac IGA1 29 213 18 11 14 21 4 8 5 0 6 18 JY8QFUQ01A2AVP 13752 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 989 95.81 206/215 nt 95.35 205/215 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 8 8 8.8.8 [0.17.38.11] CARDNYGDYW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 62 of V-REGION: 'C' (1 nucleotides at position 90 to 90 in the user submitted sequence), (cause frameshift); ctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtgttaatggtgatCgggggtagcacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [271] a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| 320 223 214 9 4 5 4 1 0 0 0 0 0 0 3 0 1 0 78 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 3 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 4 2 1 1 0 0 0 0 0 0 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac IGA2 9 213 5 4 1 5 4 4 4 0 2 3 JY8QFUQ01A2B2A 5325 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 1002 96.73 207/214 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-16*02 F 2 8 7 16 8.7.16 [1.17.38.11] CASANYDYVWGSYRYDYW in-frame - tctgggtggctccatcagtagttactactggagctggatccggcagacccccaggaagggactggagtggattgggtatatctattacagtgggagcaccaactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgtgcctcagccaattatgattacgtttgggggagttatcgttatgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [294] c75>t|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)|c133>a,P45>T(+ + -)|a138>c|g139>a,G47>R(- - -)| c75>t|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)| c133>a,P45>T(+ + -)|a138>c|g139>a,G47>R(- - -)| g315>c|a316>t,R106>S(- - -)|g317>c,R106>S(- - -)|a320>c| 314 (320) 216 (222) 209 (211) 7 (11) 2 (4) 5 (7) 0 1 2 1 1 (2) 1 0 (1) 0 0 (2) 0 0 1 78 4 0 4 1 3 0 0 2 1 0 0 0 0 0 0 0 1 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 48 3 1 2 0 1 0 0 1 1 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 (8) 2 2 (4) 0 (4) 0 (2) 0 (2) 0 0 0 0 0 (1) 0 0 (1) 0 0 (2) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctg ggtggctccatcagtagttactac tggagctggatccggcagacccccaggaagggactggagtggattgggtat atctattacagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgt gcctcagccaattatgattacgtttgggggagttatcgttatgactac ASANYDYVWGSYRYDY IGHV4-59 IGHD3-16 IGHJ4 ggtggctccatcagtagttactac tggagctggatccggcagacccccaggaagggactggagtggattgggtat atctattacagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgt gcctcagccaattatgattacgtttgggggagttatcgttatgactac IGG1 3 210 1 2 1 2 0 1 1 0 2 0 JY8QFUQ01A2BDN 15940 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 968 94.04 205/218 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-3*01 F 1 8 8 10 8.8.10 [1.17.38.11] CARGGLEPVDYW in-frame - cctctggattcaccttcagtacatactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcccgtatcaatcctgatgggcgaatcacaaactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggactcggctgtgtactactgtgcaaggggtggtttggagcccgtagattattggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [281, 280] g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|a162>c|t168>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|g216>t,K72>N(+ - -)|a295>t,T99>S(+ - +)|t306>c| g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)| a162>c| t168>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|g216>t,K72>N(+ - -)|a295>t,T99>S(+ - +)|t306>c| a318>g|a320>g| 317 (320) 223 (226) 210 (211) 13 (15) 3 (5) 10 0 (2) 1 0 2 3 1 1 1 2 0 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 1 0 0 1 0 0 0 51 51 50 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 30 24 18 6 1 5 0 0 0 1 2 0 0 1 1 0 1 0 117 114 110 4 1 3 0 1 0 1 0 0 1 0 0 0 1 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtacatactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcccgt atcaatcctgatgggcgaatcaca aactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggactcggctgtgtactactgt gcaaggggtggtttggagcccgtagattat ARGGLEPVDY IGHV3-74 IGHD3-3 IGHJ4 ggattcaccttcagtacatactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcccgt atcaatcctgatgggcgaatcaca aactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggactcggctgtgtactactgt gcaaggggtggtttggagcccgtagattat IGG2 13 213 3 10 1 6 2 7 2 1 3 7 JY8QFUQ01A2CO4 19240 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 1040 97.71 213/218 nt NA Homsap IGHJ4*02 F 240 100 48/48 nt Homsap IGHD6-13*01 F 3 8 8 11 8.8.11 [1.17.38.11] CAKGPHQYYFDYW in-frame + cctctggattcacctttagcagctatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaactattagtggtagtggtggtaggacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacactatatctgcaaatgaacagcctgagagccgaggacacggccatatattactgtgcgaaaggtccgcatcaatactactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [282] g163>a,A55>T(- - -)|c192>g,S64>R(- - -)|g258>a|g261>a|g301>a,V101>I(+ - +)| g163>a,A55>T(- - -)| c192>g,S64>R(- - -)| g258>a|g261>a|g301>a,V101>I(+ - +)| a320>g| 319 (320) 225 (226) 220 5 (6) 2 (3) 3 0 (1) 4 0 0 0 0 0 0 0 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 117 114 111 3 2 1 0 3 0 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagcagctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtggtagtggtggtaggaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacactatatctgcaaatgaacagcctgagagccgaggacacggccatatattactgt gcgaaaggtccgcatcaatactactttgactac AKGPHQYYFDY IGHV3-23 IGHD6-13 IGHJ4 ggattcacctttagcagctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtggtagtggtggtaggaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacactatatctgcaaatgaacagcctgagagccgaggacacggccatatattactgt gcgaaaggtccgcatcaatactactttgactac IGA1 5 213 4 1 4 5 0 0 2 0 2 1 JY8QFUQ01A2ECA 8666 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F (see comment) 768 82.96 185/223 nt NA Homsap IGHJ3*01 F (see comment) 187 86 43/50 nt Homsap IGHD3-9*01 F 2 8 10 13 8.10.13 [1.17.38.11] CATDSPTGYYAFDVW in-frame + Low V-REGION identity (82,96% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*01 and Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) ctctgggttcgttttcagtgatgcctggatgagttgggtccgccaggctccaggcaaggggctggagtggattggtcgtatcaagaacaaagctgacggagaggcaacagattacgccgcgcccgtgaaaggcagattcgtcatctcaagggatgactcgaaaaacatggtgtatctgcaattgaaccgcctaagagccgaggacggaggcttgtacttctgtgccacggattcccctactggatattatgcttttgatgtgtggggccgagggactcgggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [293] a81>g|a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|a106>g,N36>D(+ + -)|c108>t,N36>D(+ + -)|c120>t|g141>c|g157>a,V53>I(+ - +)|c162>t|t168>c|a171>g|g173>a,S58>N(- - -)|a178>g,T60>A(- - -)|t183>c|t186>a|g188>a,G63>E(- - -)|a190>g,T64>A(- - -)|c198>t|t204>c|a207>g|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|a240>g|t246>c|a249>g|c257>t,T86>M(- - -)|c259>g,L87>V(+ - +)|a271>t,M91>L(+ + -)|a277>c,S93>R(- - -)|g282>a|a284>g,K95>R(+ + +)|a286>g,T96>A(- - -)|a295>g,T99>G(+ - -)|c296>g,T99>G(+ - -)|c299>g,A100>G(- + -)|g301>t,V101>L(+ - +)|t306>c|a308>t,Y103>F(- + -)|a313>g,T105>A(- - -)|a318>g| a81>g|a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|a106>g,N36>D(+ + -)|c108>t,N36>D(+ + -)| c120>t|g141>c|g157>a,V53>I(+ - +)|c162>t| t168>c|a171>g|g173>a,S58>N(- - -)|a178>g,T60>A(- - -)|t183>c|t186>a|g188>a,G63>E(- - -)|a190>g,T64>A(- - -)| c198>t|t204>c|a207>g|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|a240>g|t246>c|a249>g|c257>t,T86>M(- - -)|c259>g,L87>V(+ - +)|a271>t,M91>L(+ + -)|a277>c,S93>R(- - -)|g282>a|a284>g,K95>R(+ + +)|a286>g,T96>A(- - -)|a295>g,T99>G(+ - -)|c296>g,T99>G(+ - -)|c299>g,A100>G(- + -)|g301>t,V101>L(+ - +)|t306>c|a308>t,Y103>F(- + -)| a313>g,T105>A(- - -)|a318>g| 320 231 191 40 17 23 15 4 7 5 1 0 2 1 1 3 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 3 0 2 0 0 0 0 0 0 0 0 0 51 51 47 4 3 1 0 1 2 0 0 0 0 0 1 0 0 0 30 30 22 8 4 4 3 2 0 2 0 0 0 1 0 0 0 0 117 114 93 21 8 13 7 1 3 3 1 0 2 0 0 3 1 0 8 8 6 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| ctct gggttcgttttcagtgatgcctgg atgagttgggtccgccaggctccaggcaaggggctggagtggattggtcgt atcaagaacaaagctgacggagaggcaaca gattacgccgcgcccgtgaaaggcagattcgtcatctcaagggatgactcgaaaaacatggtgtatctgcaattgaaccgcctaagagccgaggacggaggcttgtacttctgt gccacggattcccctactggatattatgcttttgatgtg ATDSPTGYYAFDV IGHV3-15 IGHD3-9 IGHJ3 gggttcgttttcagtgatgcctgg atgagttgggtccgccaggctccaggcaaggggctggagtggattggtcgt atcaagaacaaagctgacggagaggcaaca gattacgccgcgcccgtgaaaggcagattcgtcatctcaagggatgactcgaaaaacatggtgtatctgcaattgaaccgcctaagagccgaggacggaggcttgtacttctgt gccacggattcccctactggatattatgcttttgatgtg IGA1 38 219 29 9 11 16 18 22 11 5 14 8 JY8QFUQ01A2EKG 19272 productive (see comment) Homsap IGHV3-9*01 F 877 89.81 194/216 nt 88.89 192/216 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-22*01 F 2 8 8 13 8.8.13 [1.17.38.11] CAKDGDSGWDYYDHW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 52 of V-REGION: 'G' (1 nucleotides at position 69 to 69 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 67 of V-REGION: 'G' (1 nucleotides at position 107 to 107 in the user submitted sequence), (cause frameshift); tctggatttaactttgatcaatatgccatgtattgggtccggcaagctccagggaagggcctggagtgGggtctccggtatcactgggaatagtggttccataggcGtatgcggactctgtgaggggccgattcaccatctccagagacaacgccaagaagtcactatatttggaaatgaatagtctgagtgttgaggacacggccttgtatttctgtgcaaaggatggcgacagcggttgggattattatgaccactggggcccgggaactctggtcaccgtctcgtcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG4 100 88 [289] c84>t|c86>a,T29>N(- + -)|g106>c,D36>Q(+ - -)|t108>a,D36>Q(+ - -)|c118>t,H40>Y(+ - -)|c120>t,H40>Y(+ - -)|a162>c|t168>c|g170>c,S57>T(+ - +)|t172>g,W58>G(- - -)|a190>t|g191>c|a215>g,K72>R(+ + +)|c255>g,N85>K(+ - -)|c258>a|g261>a|c265>t|c268>g,Q90>E(+ + -)|c276>t|a285>t,R95>S(- - -)|c287>t,A96>V(+ - +)|a308>t,Y103>F(- + -)|a318>g| c84>t|c86>a,T29>N(- + -)|g106>c,D36>Q(+ - -)|t108>a,D36>Q(+ - -)| c118>t,H40>Y(+ - -)|c120>t,H40>Y(+ - -)|a162>c| t168>c|g170>c,S57>T(+ - +)|t172>g,W58>G(- - -)|a190>t|g191>c| a215>g,K72>R(+ + +)|c255>g,N85>K(+ - -)|c258>a|g261>a|c265>t|c268>g,Q90>E(+ + -)|c276>t|a285>t,R95>S(- - -)|c287>t,A96>V(+ - +)|a308>t,Y103>F(- + -)| a318>g|a322>g| 321 (322) 225 (226) 202 23 (24) 10 (11) 13 2 (3) 1 6 1 1 2 3 1 3 2 0 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 1 0 0 1 0 1 1 0 0 0 51 51 48 3 1 2 0 0 2 0 1 0 0 0 0 0 0 0 30 24 19 5 3 2 0 0 0 1 0 0 1 0 2 0 0 1 117 114 104 10 4 6 1 1 3 0 0 1 2 0 0 2 0 0 9 (10) 9 8 1 (2) 1 (2) 0 1 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| tct ggatttaactttgatcaatatgcc atgtattgggtccggcaagctccagggaagggcctggagtgggtctccggt atcactgggaatagtggttccata ggctatgcggactctgtgaggggccgattcaccatctccagagacaacgccaagaagtcactatatttggaaatgaatagtctgagtgttgaggacacggccttgtatttctgt gcaaaggatggcgacagcggttgggattattatgaccac AKDGDSGWDYYDH IGHV3-9 IGHD3-22 IGHJ4 ggatttaactttgatcaatatgcc atgtattgggtccggcaagctccagggaagggcctggagtgggtctccggt atcactgggaatagtggttccata ggctatgcggactctgtgaggggccgattcaccatctccagagacaacgccaagaagtcactatatttggaaatgaatagtctgagtgttgaggacacggccttgtatttctgt gcaaaggatggcgacagcggttgggattattatgaccac IGG4 22 213 9 13 7 14 2 8 5 4 8 5 JY8QFUQ01A2FAU 4432 productive (see comment) Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F or Homsap IGHV3-15*04 F 1002 94.62 211/223 nt 94.17 210/223 nt Homsap IGHJ1*01 F (see comment) 161 78.85 41/52 nt Homsap IGHD1-26*01 F 3 8 10 9 8.10.9 [1.17.38.11] CTTGGGTNYYW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR3-IMGT at codon 85 of V-REGION: 'G' (1 nucleotides at position 166 to 166 in the user submitted sequence), (cause frameshift); ctctggattcactttcagtgacgcctggatgagctgggtccgccaggctccagggaaagggctggagtgggttggccgtattccaagcaaagctgatggtgggacaacagactacgctgcgcccgttaaaggcagattcaccatctcaagagaggattcaaaaaaGtatgctgtatctgcaattgaacagcctgaaaaccgaggacacagccgtgtatttctgtaccacagggggtggaactaactactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [283] a106>g,N36>D(+ + -)|g144>a|a169>c,K57>P(- - -)|a170>c,K57>P(- - -)|a178>g,T60>A(- - -)|a207>g|g213>t|t243>g,D81>E(+ - +)|c255>t|c257>t,T86>M(- - -)|a271>t,M91>L(+ + -)|a308>t,Y103>F(- + -)| a106>g,N36>D(+ + -)| g144>a| a169>c,K57>P(- - -)|a170>c,K57>P(- - -)|a178>g,T60>A(- - -)| a207>g|g213>t|t243>g,D81>E(+ - +)|c255>t|c257>t,T86>M(- - -)|a271>t,M91>L(+ + -)|a308>t,Y103>F(- + -)| a320>g| 319 (320) 230 (231) 218 12 (13) 4 (5) 8 3 (4) 1 2 0 2 0 2 0 0 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 30 27 3 0 3 1 0 0 0 2 0 0 0 0 0 0 0 117 114 107 7 3 4 1 0 2 0 0 0 2 0 0 0 1 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| ctct ggattcactttcagtgacgcctgg atgagctgggtccgccaggctccagggaaagggctggagtgggttggccgt attccaagcaaagctgatggtgggacaaca gactacgctgcgcccgttaaaggcagattcaccatctcaagagaggattcaaaaaatatgctgtatctgcaattgaacagcctgaaaaccgaggacacagccgtgtatttctgt accacagggggtggaactaactactac TTGGGTNYY IGHV3-15 IGHD1-26 IGHJ1 ggattcactttcagtgacgcctgg atgagctgggtccgccaggctccagggaaagggctggagtgggttggccgt attccaagcaaagctgatggtgggacaaca gactacgctgcgcccgttaaaggcagattcaccatctcaagagaggattcaaaaaatatgctgtatctgcaattgaacagcctgaaaaccgaggacacagccgtgtatttctgt accacagggggtggaactaactactac IGG1 12 219 6 6 3 4 3 8 4 0 4 4 JY8QFUQ01A2FHS 14136 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 1105 100 222/222 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD5-18*01 F 3 10 7 12 10.7.12 [2.17.38.11] CARSRTFYGSANYW in-frame - gtctctggtggctccatcagcagtagtagttactactggggctggatccgccagcccccagggaaggggctggagtggattgggagtatctattatagtgggagcacctactacaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggctgtgtattactgtgcgagaagtagaaccttctatggttcggccaactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 100 100 [290] c319>a|a320>g| 318 (320) 228 (230) 228 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct ggtggctccatcagcagtagtagttactac tggggctggatccgccagcccccagggaaggggctggagtggattgggagt atctattatagtgggagcacc tactacaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggctgtgtattactgt gcgagaagtagaaccttctatggttcggccaactac ARSRTFYGSANY IGHV4-39 IGHD5-18 IGHJ4 ggtggctccatcagcagtagtagttactac tggggctggatccgccagcccccagggaaggggctggagtggattgggagt atctattatagtgggagcacc tactacaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggctgtgtattactgt gcgagaagtagaaccttctatggttcggccaactac IGG1 0 216 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01A2FU8 14265 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 888 86.13 205/238 nt 85.71 204/238 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD4-17*01 F 2 10 7 12 10.7.12 [7.17.38.11] CARRHYGEGLFDYW in-frame - Potentially, because of detected insertion/deletion in FR1-IMGT at codon 26 of V-REGION: 'C' (1 nucleotides at position 20 to 20 in the user submitted sequence), (cause frameshift); gagactctcctgcactgtcCtttggtggctccatcaccaataataattacttctggggctggatccgccagcccccagggaaggggctggagtggattggggatgtccagtatagtgggagcacctactccagcccgtccctcaagaggcgggtcaccatgtctgtggacgtgtccaaaagtcaggtctccctgagactgagctctgtgaccgccacagacacggctatttattactgtgcgagacgtcattacggtgaaggcttgtttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [306] t58>a,S20>R(- - -)|c59>g,S20>R(- - -)|c60>a,S20>R(- - -)|a64>t,T22>S(+ - +)|c77>t,S26>F(- - -)|g92>c,S31>T(+ - +)|g101>a,S34>N(- - -)|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|a113>t,Y38>F(- + -)|a163>g,S55>D(- - -)|g164>a,S55>D(- - -)|a166>g,I56>V(+ - +)|t169>c,Y57>Q(- - -)|t171>g,Y57>Q(- - -)|a200>c,Y67>S(+ - -)|a203>g,N68>S(- - -)|t222>g,S74>R(- - -)|a225>g|a234>g,I78>M(+ + -)|c237>t|a240>g|a244>g,T82>V(- - -)|c245>t,T82>V(- - -)|g252>a|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|t259>g,F87>V(+ - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|g289>a,A97>T(- - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| t58>a,S20>R(- - -)|c59>g,S20>R(- - -)|c60>a,S20>R(- - -)|a64>t,T22>S(+ - +)|c77>t,S26>F(- - -)| g92>c,S31>T(+ - +)|g101>a,S34>N(- - -)|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|a113>t,Y38>F(- + -)| a163>g,S55>D(- - -)|g164>a,S55>D(- - -)| a166>g,I56>V(+ - +)|t169>c,Y57>Q(- - -)|t171>g,Y57>Q(- - -)| a200>c,Y67>S(+ - -)|a203>g,N68>S(- - -)|t222>g,S74>R(- - -)|a225>g|a234>g,I78>M(+ + -)|c237>t|a240>g|a244>g,T82>V(- - -)|c245>t,T82>V(- - -)|g252>a|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|t259>g,F87>V(+ - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|g289>a,A97>T(- - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| a320>g| 319 (320) 245 (246) 212 33 (34) 4 (5) 29 9 (10) 8 4 1 1 1 2 1 1 1 1 3 78 22 17 5 0 5 0 0 1 0 0 1 1 1 0 1 0 0 36 30 25 5 0 5 0 3 0 0 0 0 1 0 1 0 0 0 51 51 49 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 30 21 18 3 0 3 1 0 0 1 0 0 0 0 0 0 0 1 117 114 96 18 4 14 7 4 3 0 1 0 0 0 0 0 1 2 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gagactctcctgcactgtcttt ggtggctccatcaccaataataattacttc tggggctggatccgccagcccccagggaaggggctggagtggattggggat gtccagtatagtgggagcacc tactccagcccgtccctcaagaggcgggtcaccatgtctgtggacgtgtccaaaagtcaggtctccctgagactgagctctgtgaccgccacagacacggctatttattactgt gcgagacgtcattacggtgaaggcttgtttgactac ARRHYGEGLFDY IGHV4-39 IGHD4-17 IGHJ4 ggtggctccatcaccaataataattacttc tggggctggatccgccagcccccagggaaggggctggagtggattggggat gtccagtatagtgggagcacc tactccagcccgtccctcaagaggcgggtcaccatgtctgtggacgtgtccaaaagtcaggtctccctgagactgagctctgtgaccgccacagacacggctatttattactgt gcgagacgtcattacggtgaaggcttgtttgactac IGA1 28 216 21 7 11 13 10 15 4 0 16 8 JY8QFUQ01A2G8U 2018 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F (see comment) 709 81.69 174/213 nt 81.22 173/213 nt Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD6-6*01 F 3 8 7 9 8.7.9 [1.17.37.11] CARLDGHFDLW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (81,69% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR3-IMGT, from codon 76 of V-REGION: 1 nucleotides (from position 130 in the user submitted sequence), (cause frameshift) ctctgggttcaccatcagtcactactccatggcctgggtccgccaggctccgggaaaggggctggagtgggtctcattaatttatcccggcgggagcacattccacgcagactccgtgaggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgtgcgagactcgacgggcacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [272] g88>a,V30>I(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g|a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c223>g,R75>G(- - -)|a225>c,R75>G(- - -)|c230>t,T77>I(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| g88>a,V30>I(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g| a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c223>g,R75>G(- - -)|a225>c,R75>G(- - -)|c230>t,T77>I(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| g319>c|a320>t| 318 (320) 219 (221) 180 39 (41) 7 (9) 32 8 8 4 2 4 2 2 (3) 2 2 (3) 3 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 2 0 0 2 0 1 0 0 0 0 0 51 51 45 6 2 4 2 1 0 0 0 0 0 1 1 0 1 0 30 21 17 4 2 2 0 0 0 1 1 0 0 0 1 0 0 1 117 113 89 24 3 21 6 5 4 1 1 2 1 1 0 3 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcaccatcagtcactactcc atggcctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgaggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc ARLDGHFDL IGHV3-53 IGHD6-6 IGHJ2 gggttcaccatcagtcactactcc atggcctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgaggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc IGG1 39 209 22 17 12 20 10 19 5 2 25 7 JY8QFUQ01A2JAS 17441 productive (see comment) Homsap IGHV4-4*02 F, or Homsap IGHV4-4*03 F or Homsap IGHV4-4*05 F (see comment) 671 78.44 171/218 nt 77.52 169/218 nt Homsap IGHJ1*01 F (see comment) 170 80.77 42/52 nt Homsap IGHD2-8*02 F 3 9 7 12 9.7.12 [2.16.38.11] CAREDIVREGLDHW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (78,44% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 40 of V-REGION: 'A' (1 nucleotides at position 37 to 37 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 46 of V-REGION: 1 nucleotides (from position 57 in the user submitted sequence), (cause frameshift) gtgtctggtggctccatcgccacttatcattggtggAacttgggtccgccagaccccgggaagggactggagtggattggggaagtctattatagtcgacagactaattacaacccgtccctccagagtcgcgttgacatttccattgacagtcccaacggtcagttcaccctatatctgagagatgtgaccgtcgcggacacggccgtttattattgcgcgagagaagacattgttcgggagggcctcgaccactggggccagggcaccctggtcaccgtgtcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [286] c75>g|a91>g,S31>A(- + -)|g92>c,S31>A(- + -)|g104>c,S35>T(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a109>c,N37>H(- - -)|c111>t,N37>H(- - -)|g119>c,S40>T(+ - +)|c133>a,P45>T(+ + -)|g147>a|a166>g,I56>V(+ - +)|c172>t,H58>Y(+ - -)|g187>c,G63>R(- - -)|g189>a,G63>R(- - -)|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>g,S64>Q(- - -)|c195>t|c198>t|a214>c,K72>Q(+ - -)|a225>c|c228>t|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|a234>t|a237>c|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|a245>g,K82>S(- - -)|g246>t,K82>S(- - -)|t247>c,S83>P(+ - -)|g252>c,K84>N(+ - -)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>t,N85>G(- - -)|t262>a,S88>T(+ - +)|g267>a|a268>t,K90>Y(- - -)|g270>t,K90>Y(- - -)|c276>a,S92>R(- - -)|t277>g,S93>D(- - -)|c278>a,S93>D(- - -)|c287>t,A96>V(+ - +)|g303>t|c309>t|t312>c| c75>g| a91>g,S31>A(- + -)|g92>c,S31>A(- + -)|g104>c,S35>T(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a109>c,N37>H(- - -)|c111>t,N37>H(- - -)| g119>c,S40>T(+ - +)|c133>a,P45>T(+ + -)|g147>a| a166>g,I56>V(+ - +)|c172>t,H58>Y(+ - -)|g187>c,G63>R(- - -)|g189>a,G63>R(- - -)|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>g,S64>Q(- - -)|c195>t| c198>t|a214>c,K72>Q(+ - -)|a225>c|c228>t|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|a234>t|a237>c|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|a245>g,K82>S(- - -)|g246>t,K82>S(- - -)|t247>c,S83>P(+ - -)|g252>c,K84>N(+ - -)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>t,N85>G(- - -)|t262>a,S88>T(+ - +)|g267>a|a268>t,K90>Y(- - -)|g270>t,K90>Y(- - -)|c276>a,S92>R(- - -)|t277>g,S93>D(- - -)|c278>a,S93>D(- - -)|c287>t,A96>V(+ - +)|g303>t|c309>t|t312>c| 320 226 179 47 12 35 6 6 8 2 5 4 4 1 5 2 3 1 78 6 5 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 27 20 7 0 7 1 1 1 0 1 0 1 0 2 0 0 0 51 50 47 3 1 2 0 1 0 0 0 1 0 0 1 0 0 0 30 21 13 8 1 7 1 2 2 0 1 0 0 0 1 1 0 0 117 114 86 28 9 19 4 2 5 2 3 3 3 1 1 0 3 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtgtct ggtggctccatcgccacttatcattgg tggacttgggtccgccagaccccgggaagggactggagtggattggggaa gtctattatagtcgacagact aattacaacccgtccctccagagtcgcgttgacatttccattgacagtcccaacggtcagttcaccctatatctgagagatgtgaccgtcgcggacacggccgtttattattgc gcgagagaagacattgttcgggagggcctcgaccac AREDIVREGLDH IGHV4-4 IGHD2-8 IGHJ1 ggtggctccatcgccacttatcattgg tggacttgggtccgccagaccccgggaagggactggagtggattggggaa gtctattatagtcgacagact aattacaacccgtccctccagagtcgcgttgacatttccattgacagtcccaacggtcagttcaccctatatctgagagatgtgaccgtcgcggacacggccgtttattattgc gcgagagaagacattgttcgggagggcctcgaccac IGA1 46 212 22 24 14 27 8 19 10 1 21 14 JY8QFUQ01A2MSQ 15741 productive (see comment) Homsap IGHV3-7*01 F 922 92.13 199/216 nt 91.67 198/216 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-24*01 ORF 2 8 8 12 8.8.12 [1.17.38.11] CARWRGQQSEMEYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'GT' (2 nucleotides at position 13 to 14 in the user submitted sequence), (cause frameshift); tctggattcaccGTtttagtaactattacatgagttgggtccgccaggctccagggaaggggctggagtacgtggccagcataaaacaagatgaaggtcagacatactatgcgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatcttcaaatgaacagcctgagagtcgaggacacggctgtgtatcactgtgcgcggtggagagggcaacaatccgaaatggaatactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [285] g107>a,S36>N(- - -)|g113>a,W38>Y(- + -)|g114>c,W38>Y(- + -)|c120>t|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|a164>g,N55>S(- - -)|g171>a|g185>a,G62>E(- - -)|a187>g,S63>G(+ + -)|g190>c,E64>Q(+ + -)|a194>c,K65>T(- - -)|t203>c,V68>A(+ - +)|a206>g,D69>G(- - -)|g267>t|c287>t,A96>V(+ - +)|t307>c,Y103>H(+ - -)| g107>a,S36>N(- - -)|g113>a,W38>Y(- + -)|g114>c,W38>Y(- + -)| c120>t|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|a164>g,N55>S(- - -)| g171>a|g185>a,G62>E(- - -)|a187>g,S63>G(+ + -)|g190>c,E64>Q(+ + -)|a194>c,K65>T(- - -)| t203>c,V68>A(+ - +)|a206>g,D69>G(- - -)|g267>t|c287>t,A96>V(+ - +)|t307>c,Y103>H(+ - -)| a316>c|a318>g|g319>t|a320>g| 315 (320) 219 (224) 202 (203) 17 (21) 3 (7) 14 3 (5) 5 2 2 1 (2) 0 0 0 3 0 1 (2) 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 2 0 0 0 0 0 0 1 0 0 0 51 51 47 4 1 3 1 1 1 0 0 0 0 0 1 0 0 0 30 24 19 5 1 4 1 2 0 0 1 0 0 0 1 0 0 0 117 114 109 5 1 4 1 0 1 2 0 0 0 0 0 0 1 0 3 (8) 3 3 (4) 0 (4) 0 (4) 0 0 (2) 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcacctttagtaactattac atgagttgggtccgccaggctccagggaaggggctggagtacgtggccagc ataaaacaagatgaaggtcagaca tactatgcgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatcttcaaatgaacagcctgagagtcgaggacacggctgtgtatcactgt gcgcggtggagagggcaacaatccgaaatggaatac ARWRGQQSEMEY IGHV3-7 IGHD5-24 IGHJ4 ggattcacctttagtaactattac atgagttgggtccgccaggctccagggaaggggctggagtacgtggccagc ataaaacaagatgaaggtcagaca tactatgcgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatcttcaaatgaacagcctgagagtcgaggacacggctgtgtatcactgt gcgcggtggagagggcaacaatccgaaatggaatac IGA2 17 213 12 5 7 11 5 6 2 1 7 7 JY8QFUQ01A2PDE 13208 productive Homsap IGHV3-33*05 F (see comment) 747 82.95 180/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-12*01 F 3 8 8 13 8.8.13 [1.17.38.11] CVKGRGYNDYGLDYW in-frame + Low V-REGION identity (82,95% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release gtctggattctccttcagcaattatgccatccactgggtccgccaggctccaggcaaggggctggagtgggtggcgaccatttcatatgatattaataaaagatattatgcagagtccgtgaggggccgattcaccctctccagagacaattccaagaacactctcgatctgctcatggatacccttcggttcgacgacacggctgtctattattgtgttaaaggacgtggatacaatgactacggcctcgactactggggcccgggaaccctgctcaccgtctcctcagcctccacaagggcccatcggtcttccccctggcaccctctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 71 75 [286, 287] a85>t,T29>S(+ - +)|t105>c|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g113>c,G38>A(- + -)|g117>c,M39>I(+ + -)|a162>g|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)|t165>c,V55>T(- - -)|a168>t|g184>a,G62>I(- - -)|g185>t,G62>I(- - -)|a186>t,G62>I(- - -)|g188>a,S63>N(- - -)|t192>a,N64>K(+ - -)|a194>g,K65>R(+ + +)|c198>t|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|a232>c,I78>L(+ + +)|g258>t|g261>c|t262>g,Y88>D(- - -)|a269>t,Q90>L(- - -)|a270>c,Q90>L(- - -)|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|g278>c,S93>T(+ - +)|g282>t|a283>c|a285>g|g286>t,A96>F(+ - -)|c287>t,A96>F(+ - -)|g291>c,E97>D(+ - +)|g303>c|c309>t| a85>t,T29>S(+ - +)|t105>c|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g113>c,G38>A(- + -)| g117>c,M39>I(+ + -)|a162>g|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)|t165>c,V55>T(- - -)| a168>t|g184>a,G62>I(- - -)|g185>t,G62>I(- - -)|a186>t,G62>I(- - -)|g188>a,S63>N(- - -)|t192>a,N64>K(+ - -)|a194>g,K65>R(+ + +)| c198>t|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|a232>c,I78>L(+ + +)|g258>t|g261>c|t262>g,Y88>D(- - -)|a269>t,Q90>L(- - -)|a270>c,Q90>L(- - -)|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|g278>c,S93>T(+ - +)|g282>t|a283>c|a285>g|g286>t,A96>F(+ - -)|c287>t,A96>F(+ - -)|g291>c,E97>D(+ - +)|g303>c|c309>t| c314>t,A105>V(+ - +)|g315>t,A105>V(+ - +)|g317>a,R106>K(+ + +)|a320>g| 319 (320) 224 (225) 184 40 (41) 11 (12) 29 5 (6) 5 6 3 3 0 4 1 6 1 5 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 1 1 0 0 1 0 1 0 0 0 51 51 46 5 1 4 1 1 0 2 0 0 0 0 1 0 0 0 30 24 17 7 1 6 1 2 0 0 0 0 2 1 0 0 1 0 117 114 94 20 8 12 3 0 4 0 3 0 1 0 4 1 3 1 7 (8) 7 4 3 (4) 0 (1) 3 0 (1) 1 1 0 0 0 0 0 0 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattctccttcagcaattatgcc atccactgggtccgccaggctccaggcaaggggctggagtgggtggcgacc atttcatatgatattaataaaaga tattatgcagagtccgtgaggggccgattcaccctctccagagacaattccaagaacactctcgatctgctcatggatacccttcggttcgacgacacggctgtctattattgt gttaaaggacgtggatacaatgactacggcctcgactac VKGRGYNDYGLDY IGHV3-33 IGHD5-12 IGHJ4 ggattctccttcagcaattatgcc atccactgggtccgccaggctccaggcaaggggctggagtgggtggcgacc atttcatatgatattaataaaaga tattatgcagagtccgtgaggggccgattcaccctctccagagacaattccaagaacactctcgatctgctcatggatacccttcggttcgacgacacggctgtctattattgt gttaaaggacgtggatacaatgactacggcctcgactac IGG1 37 213 17 20 9 20 8 17 9 2 16 10 JY8QFUQ01A2Q7N 13798 productive Homsap IGHV3-33*04 F (see comment) 698 80.28 175/218 nt NA Homsap IGHJ4*02 F 163 82.98 39/47 nt Homsap IGHD2-21*01 F 3 8 8 7 8.8.7 [1.17.38.11] CARDILDYW in-frame - Low V-REGION identity (80,28% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release catttggattccctttcagcgactatggcatgcactgggtccgccagactccagacaagggactagaaattgtggccattatctggcatgacggaagtcagcaattctatgcagactccgtgctgggtcgattcaccgtctccagagacaattccgacaacactctccagttgcagctgagcaggttgacagccgaagacacggctatttattattgtgcgagagatattttggactactggggccagggaacccaggtcatcgtctcttcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [271] g75>a|c77>t,S26>F(- - -)|a85>c,T29>P(+ + -)|c87>t,T29>P(+ + -)|t105>c|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g133>a,A45>T(- - -)|g140>a,G47>D(- - -)|g147>a|g153>a|t154>a,W52>I(+ - -)|g155>t,W52>I(+ - -)|g156>t,W52>I(+ - -)|a162>c|g163>a,V55>I(+ - +)|a168>c|t172>c,Y58>H(+ - -)|a190>c,N64>Q(+ - +)|t192>g,N64>Q(+ - +)|a193>c,K65>Q(+ - -)|a197>t,Y66>F(- + -)|a214>c,K72>L(- + -)|a215>t,K72>L(- + -)|c222>t|a232>g,I78>V(+ - +)|a250>g,K84>D(+ - -)|g252>c,K84>D(+ - -)|g258>t|g261>c|t262>c,Y88>Q(- - -)|t264>g,Y88>Q(- - -)|c265>t|a270>g|a271>c,M91>L(+ + -)|a275>g,N92>S(- - -)|c279>g,S93>R(- - -)|c280>t|g284>c,R95>T(- - -)|g291>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|c309>t| g75>a|c77>t,S26>F(- - -)| a85>c,T29>P(+ + -)|c87>t,T29>P(+ + -)|t105>c|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g133>a,A45>T(- - -)|g140>a,G47>D(- - -)|g147>a|g153>a|t154>a,W52>I(+ - -)|g155>t,W52>I(+ - -)|g156>t,W52>I(+ - -)|a162>c|g163>a,V55>I(+ - +)| a168>c|t172>c,Y58>H(+ - -)|a190>c,N64>Q(+ - +)|t192>g,N64>Q(+ - +)|a193>c,K65>Q(+ - -)| a197>t,Y66>F(- + -)|a214>c,K72>L(- + -)|a215>t,K72>L(- + -)|c222>t|a232>g,I78>V(+ - +)|a250>g,K84>D(+ - -)|g252>c,K84>D(+ - -)|g258>t|g261>c|t262>c,Y88>Q(- - -)|t264>g,Y88>Q(- - -)|c265>t|a270>g|a271>c,M91>L(+ + -)|a275>g,N92>S(- - -)|c279>g,S93>R(- - -)|c280>t|g284>c,R95>T(- - -)|g291>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|c309>t| 320 226 183 43 14 29 5 9 6 3 7 0 2 1 3 1 4 2 78 5 3 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 1 1 1 1 1 0 0 0 0 0 0 0 51 51 42 9 3 6 0 5 0 0 1 0 0 1 0 0 2 0 30 24 19 5 1 4 0 0 0 1 3 0 0 0 0 0 0 1 117 114 92 22 8 14 4 2 4 1 2 0 2 0 3 1 2 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cattt ggattccctttcagcgactatggc atgcactgggtccgccagactccagacaagggactagaaattgtggccatt atctggcatgacggaagtcagcaa ttctatgcagactccgtgctgggtcgattcaccgtctccagagacaattccgacaacactctccagttgcagctgagcaggttgacagccgaagacacggctatttattattgt gcgagagatattttggactac ARDILDY IGHV3-33 IGHD2-21 IGHJ4 ggattccctttcagcgactatggc atgcactgggtccgccagactccagacaagggactagaaattgtggccatt atctggcatgacggaagtcagcaa ttctatgcagactccgtgctgggtcgattcaccgtctccagagacaattccgacaacactctccagttgcagctgagcaggttgacagccgaagacacggctatttattattgt gcgagagatattttggactac IGG1 41 213 21 20 13 21 8 20 11 2 20 8 JY8QFUQ01A2QEG 10460 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30*19 F or Homsap IGHV3-30-3*02 F or Homsap IGHV3-30-5*01 F or Homsap IGHV3-33*05 F 864 88.94 193/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-15*01 F 2 8 8 13 8.8.13 [1.17.38.11] CAKDGPGGGWYAEQW in-frame + gtctggattcagtttcagtgactatgccatgcactgggtccgccagactccaggcaaggggctggagtgggtggcagttatttcatatgatgggagagagaagtactatgcagactccctgaagggccgattcaccacctccagagacaactccaagaaaatgctgtatctccaagtgaatagcctgagacctggagacacggctgtgtattactgtgcgaaagatgggccggggggtggctggtacgctgagcagtggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] c75>g|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g113>c,G38>A(- + -)|g133>a,A45>T(- - -)|a168>t|a186>g|t189>a,S63>R(- - -)|a190>g,N64>E(+ - -)|t192>g,N64>E(+ - -)|a195>g|g211>c,V71>L(+ - +)|t233>c,I78>T(- - -)|t246>c|c255>a,N85>K(+ - -)|c257>t,T86>M(- - -)|g267>c|a271>g,M91>V(+ - -)|c276>t|g286>c,A96>P(- - -)|a290>g,E97>G(- - -)|g291>a,E97>G(- - -)|g317>a,R106>K(+ + +)| c75>g| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g113>c,G38>A(- + -)| g133>a,A45>T(- - -)| a168>t|a186>g|t189>a,S63>R(- - -)|a190>g,N64>E(+ - -)|t192>g,N64>E(+ - -)|a195>g| g211>c,V71>L(+ - +)|t233>c,I78>T(- - -)|t246>c|c255>a,N85>K(+ - -)|c257>t,T86>M(- - -)|g267>c|a271>g,M91>V(+ - -)|c276>t|g286>c,A96>P(- - -)|a290>g,E97>G(- - -)|g291>a,E97>G(- - -)| g317>a,R106>K(+ + +)| 320 225 200 25 7 18 6 4 3 2 0 1 1 1 4 2 0 1 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 19 5 0 5 1 1 1 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 18 6 3 3 3 0 0 0 0 0 1 1 0 0 0 1 117 114 103 11 3 8 2 1 2 2 0 1 0 0 3 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| gtct ggattcagtttcagtgactatgcc atgcactgggtccgccagactccaggcaaggggctggagtgggtggcagtt atttcatatgatgggagagagaag tactatgcagactccctgaagggccgattcaccacctccagagacaactccaagaaaatgctgtatctccaagtgaatagcctgagacctggagacacggctgtgtattactgt gcgaaagatgggccggggggtggctggtacgctgagcag AKDGPGGGWYAEQ IGHV3-30 IGHD2-15 IGHJ4 ggattcagtttcagtgactatgcc atgcactgggtccgccagactccaggcaaggggctggagtgggtggcagtt atttcatatgatgggagagagaag tactatgcagactccctgaagggccgattcaccacctccagagacaactccaagaaaatgctgtatctccaagtgaatagcctgagacctggagacacggctgtgtattactgt gcgaaagatgggccggggggtggctggtacgctgagcag IGA1 23 213 14 9 6 12 8 11 3 3 9 8 JY8QFUQ01A2S2S 11990 productive (see comment) Homsap IGHV1-58*02 F 930 92.99 199/214 nt 92.52 198/214 nt Homsap IGHJ2*01 F 220 90.57 48/53 nt Homsap IGHD3-9*01 F 1 8 8 14 8.8.14 [0.17.38.11] CAAVLAGWGFYYFDFW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 35 of V-REGION: 'A' (1 nucleotides at position 15 to 15 in the user submitted sequence), (cause frameshift); tggattcacctttaAaaagttctgctatacagtgggtgcgacaggcccgtggacaacgccttgagtggataggatggaccgccgttggcagcggtaacacagactacgcacagaagttccaggaaagagtcaccattacgagggacatgtccacaagcacagtctacatggagatgatcaacctgggatccgaggacacggccgtgtattactgtgcggcagtcttggccggctggggattctattacttcgatttctggggccgtggcaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [288] c104>a,T35>K(- - -)|t105>a,T35>K(- - -)|c108>t|g117>a,M39>I(+ + -)|t135>c|t167>c,I56>T(- - -)|t170>c,V57>A(+ - +)|t186>c|a196>g,N66>D(+ + -)|c237>g|c260>t,A87>V(+ - +)|c271>a,L91>M(+ + -)|g275>t,S92>I(- - -)|g278>a,S93>N(- - -)|a283>g,R95>G(- - -)| c104>a,T35>K(- - -)|t105>a,T35>K(- - -)|c108>t| g117>a,M39>I(+ + -)|t135>c| t167>c,I56>T(- - -)|t170>c,V57>A(+ - +)|t186>c| a196>g,N66>D(+ + -)|c237>g|c260>t,A87>V(+ - +)|c271>a,L91>M(+ + -)|g275>t,S92>I(- - -)|g278>a,S93>N(- - -)|a283>g,R95>G(- - -)| a320>t| 319 (320) 221 (222) 206 15 (16) 4 (5) 11 2 2 2 4 0 2 0 (1) 1 0 1 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 1 0 0 1 0 1 0 0 0 0 51 51 49 2 1 1 0 1 0 1 0 0 0 0 0 0 0 0 30 24 21 3 1 2 0 0 0 3 0 0 0 0 0 0 0 0 117 114 107 7 1 6 2 1 1 0 0 1 0 0 0 1 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,2-3(FR1)|aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,105-106(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|ta,158-159(FR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|aa,221-222(FR3)|ta,234-235(FR3)|aa,252-253(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,158-159(FR2)|tt,173-174(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,132-135(FR2)|ggta,187-190(CDR2)|agtt,209-212(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggca,315-318(CDR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|aact,196-199(FR3)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggattcacctttaaaagttctgct atacagtgggtgcgacaggcccgtggacaacgccttgagtggataggatgg accgccgttggcagcggtaacaca gactacgcacagaagttccaggaaagagtcaccattacgagggacatgtccacaagcacagtctacatggagatgatcaacctgggatccgaggacacggccgtgtattactgt gcggcagtcttggccggctggggattctattacttcgatttc AAVLAGWGFYYFDF IGHV1-58 IGHD3-9 IGHJ2 ggattcacctttaaaagttctgct atacagtgggtgcgacaggcccgtggacaacgccttgagtggataggatgg accgccgttggcagcggtaacaca gactacgcacagaagttccaggaaagagtcaccattacgagggacatgtccacaagcacagtctacatggagatgatcaacctgggatccgaggacacggccgtgtattactgt gcggcagtcttggccggctggggattctattacttcgatttc IGA1 15 213 10 5 4 8 6 7 2 2 7 4 JY8QFUQ01A2WHC 17955 productive Homsap IGHV3-66*02 F 948 93.93 201/214 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD1-20*01 F 3 8 7 12 8.7.12 [1.17.38.11] CVGDRQRDNYFAYW in-frame + ctctggaatcagcgtcgggagcaactacatgaactgggtccgccaggctccggggaaggggctggagtgggtctcagttatttataccgggggtagcacatactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacaccctttatcttcaaatgaacagcctgagagctgaggacacggctgtgtacttctgtgtgggagatcgtcaaagggataactatttcgcatactggggccagggaaccctggtcaccgtctcctcaccatccccggaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctgtccagggcttcttccccca NA IGA1 100 95 [282] t82>a,F28>I(+ - -)|c86>g,T29>S(+ - +)|a103>g,S35>G(+ + -)|t105>g,S35>G(+ + -)|g119>a,S40>N(- - -)|a138>g|g173>c,S58>T(+ - +)|t177>g|c201>t|g258>c|g261>t|t306>c|a308>t,Y103>F(- + -)|c314>t,A105>V(+ - +)|a316>g,R106>G(- - -)| t82>a,F28>I(+ - -)|c86>g,T29>S(+ - +)|a103>g,S35>G(+ + -)|t105>g,S35>G(+ + -)| g119>a,S40>N(- - -)|a138>g| g173>c,S58>T(+ - +)|t177>g| c201>t|g258>c|g261>t|t306>c|a308>t,Y103>F(- + -)| c314>t,A105>V(+ - +)|a316>g,R106>G(- - -)| 318 220 205 15 6 9 3 1 2 1 0 0 1 1 2 1 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 0 0 0 0 0 0 1 0 1 0 1 51 51 49 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 30 21 19 2 1 1 0 0 0 0 0 0 0 0 1 0 0 1 117 114 109 5 4 1 0 0 1 1 0 0 1 0 1 0 1 0 6 6 4 2 0 2 1 0 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggaatcagcgtcgggagcaactac atgaactgggtccgccaggctccggggaaggggctggagtgggtctcagtt atttataccgggggtagcaca tactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacaccctttatcttcaaatgaacagcctgagagctgaggacacggctgtgtacttctgt gtgggagatcgtcaaagggataactatttcgcatac VGDRQRDNYFAY IGHV3-66 IGHD1-20 IGHJ4 ggaatcagcgtcgggagcaactac atgaactgggtccgccaggctccggggaaggggctggagtgggtctcagtt atttataccgggggtagcaca tactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacaccctttatcttcaaatgaacagcctgagagctgaggacacggctgtgtacttctgt gtgggagatcgtcaaagggataactatttcgcatac IGA1 13 210 5 8 2 6 3 7 5 1 2 5 JY8QFUQ01A2XE9 12205 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 936 92.63 201/217 nt 91.71 199/217 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 3 8 8 12 8.8.12 [1.17.38.11] CARDTGQWKDFDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'T' (1 nucleotides at position 12 to 12 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 58 of V-REGION: 'C' (1 nucleotides at position 88 to 88 in the user submitted sequence), (cause frameshift); ctctggattcaTcgttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgtagtaataCcgggggggactgacacagcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgtgcaagagatacggggcagtggaaggactttgactactggggccagggaaccctggtcaccgtctccttagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [286] c87>g|c159>g|t167>g,I56>S(- - -)|g173>c,S58>T(+ - +)|t174>g,S58>T(+ - +)|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| c87>g| c159>g| t167>g,I56>S(- - -)|g173>c,S58>T(+ - +)|t174>g,S58>T(+ - +)|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| 320 225 209 16 4 12 3 1 0 0 1 0 0 0 3 4 1 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 30 24 16 8 0 8 2 1 0 0 0 0 0 0 2 0 0 3 117 114 108 6 2 4 1 0 0 0 1 0 0 0 1 2 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgt agtaatacgggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac ARDTGQWKDFDY IGHV3-74 IGHD6-19 IGHJ4 ggattcacgttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgt agtaatacgggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac IGA2 16 213 4 12 1 9 3 7 3 1 4 8 JY8QFUQ01A2YCO 4923 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 924 91.36 201/220 nt 90.91 200/220 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD1-7*01 F 3 8 8 13 8.8.13 [2.17.38.11] CATGAGNWNYIFNYW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 62 of V-REGION: 'G' (1 nucleotides at position 96 to 96 in the user submitted sequence), (cause frameshift); ggcctctggatccaccgtcagtacctatagcctgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattagtagtagtgGgtttttatatatactacgcggattcagtgaagggccgagtcaccatctccagagacaacgccaagaattcgctatttctgcaaatgaacaacctgcgagccgaggacacggctgtctattactgtgcgacaggggccggaaactggaattacatttttaactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [291] a72>g|t83>c,F28>S(- - -)|t88>g,F30>V(+ - -)|g107>c,S36>T(+ - +)|a115>c,M39>L(+ + -)|a184>g,S62>G(+ + -)|a187>t,S63>F(- - -)|g188>t,S63>F(- - -)|c192>t|a204>g|c207>t|t226>g,F76>V(+ - -)|c255>t|a258>g|g261>a|a263>t,Y88>F(- + -)|g278>a,S93>N(- - -)|a283>c|g303>c|g317>c,R106>T(- - -)| a72>g| t83>c,F28>S(- - -)|t88>g,F30>V(+ - -)|g107>c,S36>T(+ - +)| a115>c,M39>L(+ + -)| a184>g,S62>G(+ + -)|a187>t,S63>F(- - -)|g188>t,S63>F(- - -)|c192>t| a204>g|c207>t|t226>g,F76>V(+ - -)|c255>t|a258>g|g261>a|a263>t,Y88>F(- + -)|g278>a,S93>N(- - -)|a283>c|g303>c| g317>c,R106>T(- - -)|a320>g| 319 (320) 227 (228) 207 20 (21) 9 (10) 11 4 (5) 2 3 1 2 0 2 0 3 0 1 2 78 7 6 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 1 0 0 0 0 1 0 0 1 51 51 50 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 30 24 20 4 1 3 1 0 1 0 0 0 1 0 0 0 1 0 117 114 104 10 7 3 2 2 2 0 1 0 1 0 1 0 0 1 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 0 0 0 0 0 0 1 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ggcctct ggatccaccgtcagtacctatagc ctgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtggtttttatata tactacgcggattcagtgaagggccgagtcaccatctccagagacaacgccaagaattcgctatttctgcaaatgaacaacctgcgagccgaggacacggctgtctattactgt gcgacaggggccggaaactggaattacatttttaactac ATGAGNWNYIFNY IGHV3-21 IGHD1-7 IGHJ4 ggatccaccgtcagtacctatagc ctgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtggtttttatata tactacgcggattcagtgaagggccgagtcaccatctccagagacaacgccaagaattcgctatttctgcaaatgaacaacctgcgagccgaggacacggctgtctattactgt gcgacaggggccggaaactggaattacatttttaactac IGA1 18 213 9 9 5 8 4 10 7 1 4 6 JY8QFUQ01A2YPP 13460 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 915 90.91 200/220 nt 90 198/220 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-13*01 F 1 8 8 14 8.8.14 [2.17.38.11] CATSRGSANWYPFDHW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'G' (1 nucleotides at position 13 to 13 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 91 of V-REGION: 'A' (1 nucleotides at position 180 to 180 in the user submitted sequence), (cause frameshift); agcctctggattGcaccttcagtgcctctggcataaactgggtccgccaggctccagggaaggggctggaatgggtctcatccatcactgggagtagtagtcacacattttatgcagactcagtgaagggccgattcaccatctccagagacaacgccgagaactcagtgtacctgcaaAatgaacagcctgagagacgaggacacggctgtttattactgtgcgacgagcagggggagcgccaactggtacccctttgaccactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [295] a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a110>c,Y37>S(+ - -)|a112>g,S38>G(+ + -)|g117>a,M39>I(+ + -)|g153>a|t168>c|g170>c,S57>T(+ - +)|a172>g,S58>G(+ + -)|t174>g,S58>G(+ + -)|t190>c,Y64>H(+ - -)|t194>c,I65>T(- - -)|a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|c201>t|a250>g,K84>E(+ - -)|c259>g,L87>V(+ - +)|t264>c|c287>a,A96>D(- - -)|g303>t| a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a110>c,Y37>S(+ - -)|a112>g,S38>G(+ + -)| g117>a,M39>I(+ + -)|g153>a| t168>c|g170>c,S57>T(+ - +)|a172>g,S58>G(+ + -)|t174>g,S58>G(+ + -)|t190>c,Y64>H(+ - -)|t194>c,I65>T(- - -)| a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|c201>t|a250>g,K84>E(+ - -)|c259>g,L87>V(+ - +)|t264>c|c287>a,A96>D(- - -)|g303>t| g317>c,R106>T(- - -)|a318>g,R106>T(- - -)|g319>a|a320>g| 316 (320) 224 (228) 204 20 (24) 5 (7) 15 (17) 4 (6) 2 (3) 2 4 1 1 1 0 2 (3) 1 1 1 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 2 0 0 0 1 0 0 0 1 0 0 0 51 51 49 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 30 24 18 6 1 5 1 0 0 3 0 0 0 0 1 0 0 1 117 114 106 8 3 5 1 0 2 1 0 1 1 0 0 1 1 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct ggattcaccttcagtgcctctggc ataaactgggtccgccaggctccagggaaggggctggaatgggtctcatcc atcactgggagtagtagtcacaca ttttatgcagactcagtgaagggccgattcaccatctccagagacaacgccgagaactcagtgtacctgcaaatgaacagcctgagagacgaggacacggctgtttattactgt gcgacgagcagggggagcgccaactggtacccctttgaccac ATSRGSANWYPFDH IGHV3-21 IGHD6-13 IGHJ4 ggattcaccttcagtgcctctggc ataaactgggtccgccaggctccagggaaggggctggaatgggtctcatcc atcactgggagtagtagtcacaca ttttatgcagactcagtgaagggccgattcaccatctccagagacaacgccgagaactcagtgtacctgcaaatgaacagcctgagagacgaggacacggctgtttattactgt gcgacgagcagggggagcgccaactggtacccctttgaccac IGA1 20 213 12 8 4 9 8 11 4 1 6 9 JY8QFUQ01A2YVQ 18777 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23*05 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 693 80.18 174/217 nt NA Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD5-12*01 F 3 8 8 15 8.8.15 [1.17.38.11] CAKSPDSGYDSNWLDPW in-frame + Low V-REGION identity (80,18% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctggattcaacttggcgaagttcgccatgagctgggtccgccaggctcctgggaaggggctggagtgggtctcagagatcagtggctccggtagtaaagtcggatatgcggagtccgtgaagggccgattcaccatctccaaagacaattccaagaacacattgtacttgcaaatgaccgacctgagacccggcgacacggccatttattactgtgcgaaaagtcctgatagtggctacgattctaactggctcgacccctggggcctgggaaccctggtcaccgtctcgtcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [294] c86>a,T29>N(- + -)|t90>g,F30>L(+ - -)|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|c105>g,S35>A(- + -)|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a110>t,Y37>F(- + -)|t111>c,Y37>F(- + -)|a138>t|c164>a,A55>E(- - -)|t165>g,A55>E(- - -)|t168>c|t174>c|a175>t|g176>c|t177>c|g187>a,G63>S(+ + -)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|a195>c,T65>V(- - -)|t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>a,Y66>G(+ - -)|c201>t|a204>g|c207>g,D69>E(+ - +)|g225>a|g239>a,R80>K(+ + +)|g258>a|c259>t|t264>c|c265>t|a275>c,N92>T(- + -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g286>c,A96>P(- - -)|a290>g,E97>G(- - -)|g291>c,E97>G(- - -)|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| c86>a,T29>N(- + -)|t90>g,F30>L(+ - -)|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|c105>g,S35>A(- + -)|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a110>t,Y37>F(- + -)|t111>c,Y37>F(- + -)| a138>t|c164>a,A55>E(- - -)|t165>g,A55>E(- - -)| t168>c|t174>c|a175>t|g176>c|t177>c|g187>a,G63>S(+ + -)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|a195>c,T65>V(- - -)| t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>a,Y66>G(+ - -)|c201>t|a204>g|c207>g,D69>E(+ - +)|g225>a|g239>a,R80>K(+ + +)|g258>a|c259>t|t264>c|c265>t|a275>c,N92>T(- + -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g286>c,A96>P(- - -)|a290>g,E97>G(- - -)|g291>c,E97>G(- - -)|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| g319>a|a320>g| 318 (320) 223 (225) 180 43 (45) 13 (15) 30 6 (7) 8 (9) 4 5 2 4 4 0 4 3 0 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 15 9 0 9 1 1 0 1 0 1 1 0 1 2 0 1 51 51 48 3 1 2 0 0 0 0 0 1 1 0 0 0 0 1 30 24 13 11 5 6 1 2 1 3 1 1 1 0 1 0 0 0 117 114 94 20 7 13 4 5 3 1 1 1 1 0 2 1 0 1 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaacttggcgaagttcgcc atgagctgggtccgccaggctcctgggaaggggctggagtgggtctcagag atcagtggctccggtagtaaagtc ggatatgcggagtccgtgaagggccgattcaccatctccaaagacaattccaagaacacattgtacttgcaaatgaccgacctgagacccggcgacacggccatttattactgt gcgaaaagtcctgatagtggctacgattctaactggctcgacccc AKSPDSGYDSNWLDP IGHV3-23 IGHD5-12 IGHJ5 ggattcaacttggcgaagttcgcc atgagctgggtccgccaggctcctgggaaggggctggagtgggtctcagag atcagtggctccggtagtaaagtc ggatatgcggagtccgtgaagggccgattcaccatctccaaagacaattccaagaacacattgtacttgcaaatgaccgacctgagacccggcgacacggccatttattactgt gcgaaaagtcctgatagtggctacgattctaactggctcgacccc IGG1 43 213 23 20 12 23 11 20 8 5 15 15 JY8QFUQ01A2ZLE 3255 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 883 89.5 196/219 nt NA Homsap IGHJ5*02 F 228 94.12 48/51 nt Homsap IGHD6-19*01 F 2 8 8 17 8.8.17 [2.17.38.11] CAKSPLSVAGTRQNWFDPW in-frame - ggctctgacttcacctttaatagctatgccatggcctgggtccgccaggctccagggaaggggctggagtgggtctcggctattggtgccagtggctacagcacatactacgcagactccgtcaagggccgcttcaccatctccagagaccattccaacagcacgctgcatctgcaaatgaacagcctgagagccgaagacacggccgtttattactgtgcgaaatcccctctatcagtggctggtacacgccaaaactggttcgacccctggggccagggaaccctggtcatcgtttcctcagcctccaccaagggcccatcggtnttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 88 [302] c74>g,A25>G(- + -)|g80>a,G27>D(- - -)|a81>c,G27>D(- - -)|g104>a,S35>N(- - -)|c105>t,S35>N(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a162>g|a169>g,S57>G(+ + -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|t186>c|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|t189>c,G63>Y(+ - -)|g213>c|g225>c|a244>c,N82>H(- - -)|g252>c,K84>N(+ - -)|a254>g,N85>S(- - -)|t262>c,Y88>H(+ - -)|g291>a|a303>t| c74>g,A25>G(- + -)| g80>a,G27>D(- - -)|a81>c,G27>D(- - -)|g104>a,S35>N(- - -)|c105>t,S35>N(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a162>g| a169>g,S57>G(+ + -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|t186>c|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|t189>c,G63>Y(+ - -)| g213>c|g225>c|a244>c,N82>H(- - -)|g252>c,K84>N(+ - -)|a254>g,N85>S(- - -)|t262>c,Y88>H(+ - -)|g291>a|a303>t| g319>t|a320>c| 318 (320) 225 (227) 202 23 (25) 6 (8) 17 4 4 1 4 2 (3) 0 1 0 5 1 1 (2) 0 78 6 5 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 36 24 20 4 0 4 0 2 1 0 1 0 0 0 0 0 0 0 51 51 48 3 1 2 2 0 0 0 0 0 0 0 1 0 0 0 30 24 17 7 1 6 1 1 0 3 0 0 0 0 1 0 1 0 117 114 106 8 4 4 1 1 0 1 1 0 1 0 3 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ggctct gacttcacctttaatagctatgcc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcggct attggtgccagtggctacagcaca tactacgcagactccgtcaagggccgcttcaccatctccagagaccattccaacagcacgctgcatctgcaaatgaacagcctgagagccgaagacacggccgtttattactgt gcgaaatcccctctatcagtggctggtacacgccaaaactggttcgacccc AKSPLSVAGTRQNWFDP IGHV3-23 IGHD6-19 IGHJ5 gacttcacctttaatagctatgcc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcggct attggtgccagtggctacagcaca tactacgcagactccgtcaagggccgcttcaccatctccagagaccattccaacagcacgctgcatctgcaaatgaacagcctgagagccgaagacacggccgtttattactgt gcgaaatcccctctatcagtggctggtacacgccaaaactggttcgacccc IGG1 22 213 13 9 5 11 8 11 5 1 6 10 JY8QFUQ01A30CD 12919 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 801 85.71 186/217 nt NA Homsap IGHJ6*02 F (see comment) 193 79.03 49/62 nt Homsap IGHD6-19*01 F 1 8 8 17 8.8.17 [1.17.38.11] CVRDSDSSGYARYDSMDVW in-frame + Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ctctggattcaacctcaatacctttggcatgaactgggtccgccaggcgccagggaagggactggagtgggtctcacacgtcaatcggggtagtactcacatatactacgcaggctcagtgaggggccggttcaccatctccagagacgacgccgggaactcagtctatctgcaaatgaatagcctgagagccgaggacacgggtttatattattgtgtgagagacagtgactcgtccggctacgcgaggtacgactctatggacgtctggggccaagggaccgcggtcaccgtctcatcagcctccagccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 93 88 [301] c86>a,T29>N(- + -)|t88>c,F30>L(+ - -)|g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|a112>g,S38>G(+ + -)|t135>g|g147>a|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>a,S57>N(- - -)|a172>c,S58>R(- - -)|t174>g,S58>R(- - -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)|a206>g,D69>G(- - -)|a215>g,K72>R(+ + +)|a225>g|a244>g,N82>D(+ + -)|a250>g,K84>G(- - -)|a251>g,K84>G(- - -)|c259>g,L87>V(+ - +)|g261>c,L87>V(+ - +)|c276>t|c299>g,A100>G(- + -)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|c309>t|c314>t,A105>V(+ - +)| c86>a,T29>N(- + -)|t88>c,F30>L(+ - -)|g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|a112>g,S38>G(+ + -)| t135>g|g147>a|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>a,S57>N(- - -)|a172>c,S58>R(- - -)|t174>g,S58>R(- - -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)| a206>g,D69>G(- - -)|a215>g,K72>R(+ + +)|a225>g|a244>g,N82>D(+ + -)|a250>g,K84>G(- - -)|a251>g,K84>G(- - -)|c259>g,L87>V(+ - +)|g261>c,L87>V(+ - +)|c276>t|c299>g,A100>G(- + -)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|c309>t| c314>t,A105>V(+ - +)| 320 225 193 32 5 27 9 4 3 4 1 2 1 0 3 2 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 1 0 1 0 1 1 0 1 0 0 0 51 51 47 4 2 2 0 1 0 1 0 1 0 0 0 0 0 1 30 24 16 8 0 8 2 1 0 2 1 0 0 0 1 0 0 1 117 114 101 13 3 10 6 1 2 0 0 0 0 0 1 2 1 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaacctcaatacctttggc atgaactgggtccgccaggcgccagggaagggactggagtgggtctcacac gtcaatcggggtagtactcacata tactacgcaggctcagtgaggggccggttcaccatctccagagacgacgccgggaactcagtctatctgcaaatgaatagcctgagagccgaggacacgggtttatattattgt gtgagagacagtgactcgtccggctacgcgaggtacgactctatggacgtc VRDSDSSGYARYDSMDV IGHV3-21 IGHD6-19 IGHJ6 ggattcaacctcaatacctttggc atgaactgggtccgccaggcgccagggaagggactggagtgggtctcacac gtcaatcggggtagtactcacata tactacgcaggctcagtgaggggccggttcaccatctccagagacgacgccgggaactcagtctatctgcaaatgaatagcctgagagccgaggacacgggtttatattattgt gtgagagacagtgactcgtccggctacgcgaggtacgactctatggacgtc IGG2 31 213 19 12 6 14 13 17 5 0 12 14 JY8QFUQ01A312M 15735 productive Homsap IGHV3-7*01 F 1035 97.7 212/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD4-23*01 ORF 2 8 8 12 8.8.12 [1.17.38.11] CVSSPYFYDSNGYW in-frame - ctctggattcaggtttagcatctattggatgagctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagcaagatggaagtgagaaatactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagcagcctgagagccgaggacacggctgtgtattactgtgtgagttcaccgtatttctatgatagtaatggttactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [284] c86>g,T29>R(- - -)|c87>g,T29>R(- - -)|t105>c|g107>t,S36>I(- - -)|a275>g,N92>S(- - -)|c314>t,A105>V(+ - +)| c86>g,T29>R(- - -)|c87>g,T29>R(- - -)|t105>c|g107>t,S36>I(- - -)| a275>g,N92>S(- - -)| c314>t,A105>V(+ - +)|a318>t,R106>S(- - -)|g319>t|a320>c| 317 (320) 222 (225) 216 6 (9) 1 (3) 5 (6) 1 0 1 1 0 (1) 0 0 (1) 0 0 2 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 0 1 0 0 0 0 0 2 1 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 113 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 5 (8) 5 4 1 (4) 0 (2) 1 (2) 0 0 1 0 0 (1) 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaggtttagcatctattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaagtgagaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagcagcctgagagccgaggacacggctgtgtattactgt gtgagttcaccgtatttctatgatagtaatggttac VSSPYFYDSNGY IGHV3-7 IGHD4-23 IGHJ4 ggattcaggtttagcatctattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaagtgagaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagcagcctgagagccgaggacacggctgtgtattactgt gtgagttcaccgtatttctatgatagtaatggttac IGA2 5 213 2 3 0 3 2 2 0 1 1 3 JY8QFUQ01A313A 20137 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 923 91.28 199/218 nt 90.83 198/218 nt Homsap IGHJ1*01 F (see comment) 142 76 38/50 nt Homsap IGHD5-18*01 F 3 8 8 6 8.8.6 [1.17.38.11] CASYGQWL in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 55 of V-REGION: 'AGT' (3 nucleotides at position 78 to 80 in the user submitted sequence), (do not cause frameshift); cctcgggactcatgtttagcagctatgccatgacctgggtccgccaggctccagggaaggggctggagtgggtctcaAGTgtcagtagtagtactggttatttcacatactacacagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtatattattgcgctagttacgggcagtggctgggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [271] t78>g|t82>c,F28>L(+ - -)|c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|g119>c,S40>T(+ - +)|c164>t,A55>V(+ - +)|t165>c,A55>V(+ - +)|t167>g,I56>S(- - -)|g172>a,G58>S(+ + -)|g176>c,S59>T(+ - +)|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)|g202>a,A68>T(- - -)|a275>g,N92>S(- - -)|g291>a|c309>t|t312>c| t78>g| t82>c,F28>L(+ - -)|c86>t,T29>M(- - -)|c87>g,T29>M(- - -)| g119>c,S40>T(+ - +)|c164>t,A55>V(+ - +)|t165>c,A55>V(+ - +)| t167>g,I56>S(- - -)|g172>a,G58>S(+ + -)|g176>c,S59>T(+ - +)|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)| g202>a,A68>T(- - -)|a275>g,N92>S(- - -)|g291>a|c309>t|t312>c| g315>t|a317>g,K106>S(- - -)|a318>t,K106>S(- - -)|g319>t| 314 (320) 220 (226) 201 (203) 19 (23) 4 (6) 15 (17) 1 (2) 4 3 3 0 0 1 (2) 0 2 1 2 (4) 2 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 36 24 21 3 0 3 0 0 1 1 0 0 0 0 0 1 0 0 51 51 48 3 0 3 0 0 1 1 0 0 0 0 1 0 0 0 30 24 17 7 0 7 0 2 0 0 0 0 1 0 1 0 2 1 117 114 109 5 3 2 1 2 1 1 0 0 0 0 0 0 0 0 2 (8) 2 2 (4) 0 (4) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (2) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctcg ggactcatgtttagcagctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcagtc agtagtagtactggttatttcaca tactacacagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtatattattgc gctagttacgggcagtgg ASYGQW IGHV3-23 IGHD5-18 IGHJ1 ggactcatgtttagcagctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcagtc agtagtagtactggttatttcaca tactacacagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtatattattgc gctagttacgggcagtgg IGG2 18 213 11 7 7 12 4 6 3 0 5 10 JY8QFUQ01A33TY 17349 productive (see comment) Homsap IGHV4-61*02 F 957 92.38 206/223 nt 91.93 205/223 nt Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD3-10*01 F 3 10 7 11 10.7.11 [2.17.38.11] CATVARRGAFDIW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 95 of V-REGION: 'G' (1 nucleotides at position 195 to 195 in the user submitted sequence), (cause frameshift); gtcgtctggtgggtccatcagcagtactagttactactggagctggatccggcagcccgccgggaagggactggagtggatggggcgtatctataccagtgggatcaccaactacaacccctccctcgagagtcgagtcaccttttcagtggacacgtccaagaaccagttctccctgaagctgaagtctgtgaGcccccgcagacacggccgtttattactgtgcgacagtggcgcgccggggggcttttgatatctggggccaagggacactggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccccctggcgccctgctccaggagcacctccgagagcacagcagccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 93 94 [290, 289] t72>g|g73>t,V25>S(- - -)|t74>c,V25>S(- - -)|c75>g,V25>S(- - -)|c84>g|g103>a,G35>T(+ - -)|g104>c,G35>T(+ - -)|t159>g,I53>M(+ + -)|g191>t,S64>I(- - -)|a214>g,K72>E(+ - -)|a232>t,I78>F(+ - -)|a234>t,I78>F(+ - -)|a240>g|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g286>c,A96>P(- - -)|g303>t|g317>c,R106>T(- - -)| t72>g|g73>t,V25>S(- - -)|t74>c,V25>S(- - -)|c75>g,V25>S(- - -)| c84>g|g103>a,G35>T(+ - -)|g104>c,G35>T(+ - -)| t159>g,I53>M(+ + -)| g191>t,S64>I(- - -)| a214>g,K72>E(+ - -)|a232>t,I78>F(+ - -)|a234>t,I78>F(+ - -)|a240>g|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g286>c,A96>P(- - -)|g303>t| g317>c,R106>T(- - -)|a320>t| 319 (320) 230 (231) 212 18 (19) 4 (5) 14 2 2 0 1 0 0 2 (3) 0 3 3 3 2 78 7 3 4 1 3 0 0 0 1 0 0 0 0 0 1 1 1 36 30 27 3 1 2 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 30 21 20 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 117 114 106 8 2 6 2 1 0 0 0 0 2 0 1 1 1 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 0 0 0 0 0 (1) 0 1 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtcgtct ggtgggtccatcagcagtactagttactac tggagctggatccggcagcccgccgggaagggactggagtggatggggcgt atctataccagtgggatcacc aactacaacccctccctcgagagtcgagtcaccttttcagtggacacgtccaagaaccagttctccctgaagctgaagtctgtgacccccgcagacacggccgtttattactgt gcgacagtggcgcgccggggggcttttgatatc ATVARRGAFDI IGHV4-61 IGHD3-10 IGHJ3 ggtgggtccatcagcagtactagttactac tggagctggatccggcagcccgccgggaagggactggagtggatggggcgt atctataccagtgggatcacc aactacaacccctccctcgagagtcgagtcaccttttcagtggacacgtccaagaaccagttctccctgaagctgaagtctgtgacccccgcagacacggccgtttattactgt gcgacagtggcgcgccggggggcttttgatatc IGG2 13 216 4 9 2 8 2 5 2 1 7 3 JY8QFUQ01A33U2 19415 productive (see comment) Homsap IGHV4-39*03 F 893 89.55 197/220 nt 88.64 195/220 nt Homsap IGHJ3*01 F 232 96 48/50 nt Homsap IGHD3-22*01 F 3 10 7 17 10.7.17 [1.17.38.11] CARSITLTVVVMNDAFDVW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'A' (1 nucleotides at position 28 to 28 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 51 of V-REGION: 'TGG' (3 nucleotides at position 73 to 75 in the user submitted sequence), (do not cause frameshift); tctccggtggctccatcaacagtagaaAattattattggggctggatccgccagccccccagggaagggtttTGGgagtggattggaaatatctattatagtgggaacacctactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgtgcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtctggggccaagggacattggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 86 94 [308] t78>c|g92>a,S31>N(- - -)|t105>a,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t|c114>t|a138>c|g139>a,G47>R(- - -)|a142>g,K48>E(+ - -)|g144>a,K48>E(+ - -)|c148>t,L50>F(+ - -)|g150>t,L50>F(+ - -)|g162>a|g164>a,S55>N(- - -)|g191>a,S64>N(- - -)|c204>t|a232>g,I78>V(+ - +)|c245>g,T82>R(- - -)|c249>t|c261>g,F87>L(+ - -)|g275>c,S92>T(+ - +)|g280>c,V94>L(+ - +)|g303>a| t78>c| g92>a,S31>N(- - -)|t105>a,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t|c114>t| a138>c|g139>a,G47>R(- - -)|a142>g,K48>E(+ - -)|g144>a,K48>E(+ - -)|c148>t,L50>F(+ - -)|g150>t,L50>F(+ - -)|g162>a|g164>a,S55>N(- - -)| g191>a,S64>N(- - -)| c204>t|a232>g,I78>V(+ - +)|c245>g,T82>R(- - -)|c249>t|c261>g,F87>L(+ - -)|g275>c,S92>T(+ - +)|g280>c,V94>L(+ - +)|g303>a| 311 220 197 23 8 15 2 8 5 1 1 0 0 1 2 2 1 0 78 5 4 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 30 25 5 2 3 0 2 2 0 0 0 0 1 0 0 0 0 51 51 43 8 2 6 1 4 1 0 1 0 0 0 0 0 1 0 30 21 20 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 116 114 105 8 3 5 1 1 2 0 0 0 0 0 2 2 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctcc ggtggctccatcaacagtagaaattattat tggggctggatccgccagccccccagggaagggtttgagtggattggaaat atctattatagtgggaacacc tactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgt gcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtc ARSITLTVVVMNDAFDV IGHV4-39 IGHD3-22 IGHJ3 ggtggctccatcaacagtagaaattattat tggggctggatccgccagccccccagggaagggtttgagtggattggaaat atctattatagtgggaacacc tactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgt gcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtc IGG1 22 216 15 7 13 18 2 4 5 2 11 4 JY8QFUQ01A33ZY 20015 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 883 89.5 196/219 nt 88.58 194/219 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-10*01 F 3 8 8 10 8.8.10 [2.17.38.11] CSKDTFGREDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 47 of V-REGION: 'G' (1 nucleotides at position 56 to 56 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 79 of V-REGION: 'TA' (2 nucleotides at position 144 to 145 in the user submitted sequence), (cause frameshift); gcctctggattctccgtcagtaattactggatgcactgggtccgccaggctccagGgggaggggctggagtgggtctcacgtattaatgaagatggtagtcggacagactacgcggactccgtgaagggccgattcaccatttTAacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgtagtaaagacacatttgggcgagaagactactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggttttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccga NA IGA1 100 89 [283] a85>t,T29>S(+ - +)|t88>g,F30>V(+ - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a132>g|a142>g,K48>E(+ - -)|t152>a,V51>E(- + -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>t|a190>c,S64>R(- - -)|c192>g,S64>R(- - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c234>t|c236>a,S79>Y(+ - -)|a245>g,N82>S(- - -)|g258>a|c287>t,A96>V(+ - +)|g291>a|g303>c|c309>t| a85>t,T29>S(+ - +)|t88>g,F30>V(+ - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a132>g|a142>g,K48>E(+ - -)|t152>a,V51>E(- + -)| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>t|a190>c,S64>R(- - -)|c192>g,S64>R(- - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c234>t|c236>a,S79>Y(+ - -)|a245>g,N82>S(- - -)|g258>a|c287>t,A96>V(+ - +)|g291>a|g303>c|c309>t| g313>a,A105>S(- + -)|c314>g,A105>S(- + -)|a315>t,A105>S(- + -)|g317>a,R106>K(+ + +)| 320 227 200 27 7 20 5 7 4 0 1 1 2 2 1 2 1 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 1 0 0 0 1 0 0 0 0 1 51 51 48 3 1 2 2 0 0 0 0 0 0 1 0 0 0 0 30 24 18 6 1 5 1 1 0 0 1 0 0 1 0 1 1 0 117 114 104 10 5 5 2 3 3 0 0 1 0 0 1 0 0 0 8 8 4 4 0 4 0 2 0 0 0 0 1 0 0 1 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattctccgtcagtaattactgg atgcactgggtccgccaggctccaggggaggggctggagtgggtctcacgt attaatgaagatggtagtcggaca gactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgt agtaaagacacatttgggcgagaagactac SKDTFGREDY IGHV3-74 IGHD3-10 IGHJ4 ggattctccgtcagtaattactgg atgcactgggtccgccaggctccaggggaggggctggagtgggtctcacgt attaatgaagatggtagtcggaca gactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgt agtaaagacacatttgggcgagaagactac IGA1 23 213 14 9 9 13 5 10 6 1 7 9 JY8QFUQ01A3552 21529 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 829 86.76 190/219 nt 86.3 189/219 nt Homsap IGHJ5*02 F 129 72.55 37/51 nt Homsap IGHD5-18*01 F 2 8 8 16 8.8.16 [2.17.38.11] CVKDWGPNRQMWFGESLW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 62 of V-REGION: 'GCTGTTGCC' (9 nucleotides at position 94 to 102 in the user submitted sequence), (do not cause frameshift); gcctctggattcacgttcagcagctatgccatgacctgggtccgccagactccagggaaggggctggagtgggtctcaactattcatggcggtGCTGTTGCCggtggcaccacagactacgcggtctccgcccagggtcgattcaccatctccagagacaattccaagagcacactgtatttgcaaatgagcgacctgagacccgaggacacggccctctatttctgtgtgaaagactggggccccaatcggcagatgtggttcggggagagtctctggggccagggaactctggtcaccgtctccacagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccca NA IGA1 100 100 [307] c87>g|t90>c|g119>c,S40>T(+ - +)|g133>a,A45>T(- - -)|g163>a,A55>T(- - -)|a169>c,S57>H(+ - -)|g170>a,S57>H(+ - -)|t174>c|a175>g,S59>G(+ + -)|t189>c|g191>c,S64>T(+ - +)|t196>g,Y66>D(- - -)|a204>g|a206>t,D69>V(- - -)|t212>c,V71>A(+ - +)|g213>c,V71>A(+ - +)|a214>c,K72>Q(+ - -)|c222>t|g225>a|a254>g,N85>S(- - -)|g258>a|c265>t|a275>g,N92>S(- - -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g286>c,A96>P(- - -)|g301>c,V101>L(+ - +)|a303>c,V101>L(+ - +)|a308>t,Y103>F(- + -)|c314>t,A105>V(+ - +)| c87>g|t90>c| g119>c,S40>T(+ - +)|g133>a,A45>T(- - -)|g163>a,A55>T(- - -)| a169>c,S57>H(+ - -)|g170>a,S57>H(+ - -)|t174>c|a175>g,S59>G(+ + -)|t189>c|g191>c,S64>T(+ - +)| t196>g,Y66>D(- - -)|a204>g|a206>t,D69>V(- - -)|t212>c,V71>A(+ - +)|g213>c,V71>A(+ - +)|a214>c,K72>Q(+ - -)|c222>t|g225>a|a254>g,N85>S(- - -)|g258>a|c265>t|a275>g,N92>S(- - -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g286>c,A96>P(- - -)|g301>c,V101>L(+ - +)|a303>c,V101>L(+ - +)|a308>t,Y103>F(- + -)| c314>t,A105>V(+ - +)| 320 227 197 30 9 21 5 6 3 4 3 0 2 0 5 1 0 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 2 0 0 0 0 1 0 0 0 0 0 1 0 0 51 51 48 3 0 3 0 2 0 0 0 0 0 0 1 0 0 0 30 24 18 6 2 4 1 1 0 2 1 0 0 0 1 0 0 0 117 114 96 18 5 13 4 3 2 1 2 0 2 0 3 0 0 1 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacgttcagcagctatgcc atgacctgggtccgccagactccagggaaggggctggagtgggtctcaact attcatggcggtggtggcaccaca gactacgcggtctccgcccagggtcgattcaccatctccagagacaattccaagagcacactgtatttgcaaatgagcgacctgagacccgaggacacggccctctatttctgt gtgaaagactggggccccaatcggcagatgtggttcggggagagtctc VKDWGPNRQMWFGESL IGHV3-23 IGHD5-18 IGHJ5 ggattcacgttcagcagctatgcc atgacctgggtccgccagactccagggaaggggctggagtgggtctcaact attcatggcggtggtggcaccaca gactacgcggtctccgcccagggtcgattcaccatctccagagacaattccaagagcacactgtatttgcaaatgagcgacctgagacccgaggacacggccctctatttctgt gtgaaagactggggccccaatcggcagatgtggttcggggagagtctc IGA1 29 213 17 12 8 14 9 15 5 4 16 4 JY8QFUQ01A35C3 20833 productive Homsap IGHV4-34*06 F (see comment) 701 80.84 173/214 nt NA Homsap IGHJ4*02 F (see comment) 168 83.33 40/48 nt Homsap IGHD2-15*01 F 2 8 7 14 8.7.14 [1.17.38.11] CSRGGQSAGWHSFEAW in-frame + Low V-REGION identity (80,84% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) tccatggtgggtccctcaggggttacccctggacctggatccgccacaccgcagagaagggactggagtggattggtcaaatcaatagtgatggaaggacaacctacaactcggccctcatgggtcgagtcaccatttcaacagacacatccaagaatcagttctcgctgactgtggtttctgttgtcgccgcggacacggcaatgtattattgttcgagaggaggccaaagtgctggctggcactcctttgaagcctggggccagggaacccaggtaatcgtcacctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [288] t76>c,Y26>H(+ - -)|t88>c,F30>L(+ - -)|t105>g,S35>R(- - -)|t112>c,Y38>P(+ - -)|a113>c,Y38>P(+ - -)|g119>c,S40>T(+ - +)|g132>c,Q44>H(- + -)|c133>a,P45>T(+ + -)|c136>g,P46>A(- - -)|g140>a,G47>E(- - -)|g147>a|g162>t|g163>c,E55>Q(+ + -)|c172>a,H58>S(+ - -)|a173>g,H58>S(+ - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|c192>g,S64>R(- - -)|c195>a|a197>c,N66>T(- + -)|c205>t,P69>S(+ - -)|t208>g,S70>A(- + -)|a215>t,K72>M(- + -)|a220>g,S74>G(+ + -)|a234>t|g238>a,V80>T(- - -)|t239>c,V80>T(- - -)|g246>a|c255>t|c264>g|a269>c,K90>T(- - -)|g270>t,K90>T(- - -)|c271>g,L91>V(+ - +)|g275>t,G92>V(- - -)|c276>t,G92>V(- - -)|g282>t|a283>g,T95>V(- - -)|c284>t,T95>V(- - -)|c300>a|g301>a,V101>M(+ - -)|c309>t| t76>c,Y26>H(+ - -)| t88>c,F30>L(+ - -)|t105>g,S35>R(- - -)|t112>c,Y38>P(+ - -)|a113>c,Y38>P(+ - -)| g119>c,S40>T(+ - +)|g132>c,Q44>H(- + -)|c133>a,P45>T(+ + -)|c136>g,P46>A(- - -)|g140>a,G47>E(- - -)|g147>a|g162>t|g163>c,E55>Q(+ + -)| c172>a,H58>S(+ - -)|a173>g,H58>S(+ - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|c192>g,S64>R(- - -)|c195>a| a197>c,N66>T(- + -)|c205>t,P69>S(+ - -)|t208>g,S70>A(- + -)|a215>t,K72>M(- + -)|a220>g,S74>G(+ + -)|a234>t|g238>a,V80>T(- - -)|t239>c,V80>T(- - -)|g246>a|c255>t|c264>g|a269>c,K90>T(- - -)|g270>t,K90>T(- - -)|c271>g,L91>V(+ - +)|g275>t,G92>V(- - -)|c276>t,G92>V(- - -)|g282>t|a283>g,T95>V(- - -)|c284>t,T95>V(- - -)|c300>a|g301>a,V101>M(+ - -)|c309>t| 311 214 173 41 10 31 4 6 5 4 3 4 2 0 3 4 4 2 78 5 4 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 2 1 0 0 0 0 0 0 1 51 51 43 8 2 6 0 2 0 0 0 1 0 0 3 1 1 0 30 21 15 6 1 5 2 1 0 0 0 2 0 0 0 1 0 0 116 114 91 22 7 15 2 3 5 1 2 1 2 0 0 2 3 1 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|ggct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| tccat ggtgggtccctcaggggttacccc tggacctggatccgccacaccgcagagaagggactggagtggattggtcaa atcaatagtgatggaaggaca acctacaactcggccctcatgggtcgagtcaccatttcaacagacacatccaagaatcagttctcgctgactgtggtttctgttgtcgccgcggacacggcaatgtattattgt tcgagaggaggccaaagtgctggctggcactcctttgaagcc SRGGQSAGWHSFEA IGHV4-34 IGHD2-15 IGHJ4 ggtgggtccctcaggggttacccc tggacctggatccgccacaccgcagagaagggactggagtggattggtcaa atcaatagtgatggaaggaca acctacaactcggccctcatgggtcgagtcaccatttcaacagacacatccaagaatcagttctcgctgactgtggtttctgttgtcgccgcggacacggcaatgtattattgt tcgagaggaggccaaagtgctggctggcactcctttgaagcc IGA1 40 210 18 22 11 26 7 14 9 1 21 9 JY8QFUQ01A35F3 16698 productive Homsap IGHV3-7*01 F 954 93.55 203/217 nt NA Homsap IGHJ5*02 F (see comment) 138 74.51 38/51 nt NA 8 8 9 8.8.9 [1.17.38.11] CTTTLVWFGEL (see Functionality comment) in-frame - IMGT/JunctionAnalysis gives no results for this JUNCTION Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctggattcacgtttagagactattggatgagttgggtccgccaggctcctgggagggggctggagtgggtggccaacataaagcaagatgcaagtgaggaatactatgtggactctgtgaagggccggttcaccatctccagagacaacgccaagagctcactgcatttgcaaatgaacagcctgagagccgaggacacggctatgtattactgtacgaccacattagtatggttcggggagttaggccagggaacccaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [275] c87>g|t105>a,S35>R(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c120>t|a138>t|a143>g,K48>R(+ + +)|g185>c,G62>A(- + -)|a193>g,K65>E(+ - -)|a225>g|a254>g,N85>S(- - -)|t262>c,Y88>H(+ - -)|c265>t|g301>a,V101>M(+ - -)|g313>a,A105>T(- - -)| c87>g|t105>a,S35>R(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| c120>t|a138>t|a143>g,K48>R(+ + +)| g185>c,G62>A(- + -)|a193>g,K65>E(+ - -)| a225>g|a254>g,N85>S(- - -)|t262>c,Y88>H(+ - -)|c265>t|g301>a,V101>M(+ - -)| g313>a,A105>T(- - -)|g317>c,R106>T(- - -)|a318>c,R106>T(- - -)|g319>a|a320>c| 316 (320) 221 (225) 206 15 (19) 5 (7) 10 (12) 5 3 (4) 2 1 0 (2) 0 1 1 1 (2) 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 0 0 0 0 0 1 0 1 0 0 51 51 48 3 2 1 1 0 1 0 0 0 1 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 117 114 109 5 2 3 2 1 1 1 0 0 0 0 0 0 0 0 4 (8) 4 3 1 (5) 0 (2) 1 (3) 0 1 (2) 0 0 0 (2) 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgtttagagactattgg atgagttgggtccgccaggctcctgggagggggctggagtgggtggccaac ataaagcaagatgcaagtgaggaa tactatgtggactctgtgaagggccggttcaccatctccagagacaacgccaagagctcactgcatttgcaaatgaacagcctgagagccgaggacacggctatgtattactgt acgaccacattagtatggttcggggag TTTLVWFGE IGHV3-7 IGHJ5 ggattcacgtttagagactattgg atgagttgggtccgccaggctcctgggagggggctggagtgggtggccaac ataaagcaagatgcaagtgaggaa tactatgtggactctgtgaagggccggttcaccatctccagagacaacgccaagagctcactgcatttgcaaatgaacagcctgagagccgaggacacggctatgtattactgt acgaccacattagtatggttcggggag IGA1 14 213 10 4 4 6 6 8 4 1 4 5 JY8QFUQ01A35NF 13597 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 884 89.14 197/221 nt 88.69 196/221 nt Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD3-10*02 F 2 10 7 11 10.7.11 [1.17.38.11] CVRHVRRFWFDPW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 55 to 55 in the user submitted sequence), (cause frameshift); tctctaatggctccatcagcggaagtgtttactactgggcctggatccgccagcCccccagagaagggtctggagtacattggaagcatcttttatagtgggagcacttacttcaatccgtccctcaagagtcgagtcaccctatccgtagacacgtccaggaaccagttctccctgaggctgaagtctgtgaccgccgcagacacggctgtttattattgtgtgagacatgttcgacgattctggttcgacccctggggccagggaaccctggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [286] g79>a,G27>N(- - -)|g80>a,G27>N(- - -)|a100>g,S34>G(+ + -)|t102>a,S34>G(+ + -)|a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|g119>c,G40>A(- + -)|g140>a,G47>E(- - -)|g147>t|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|g162>a|t165>c|a170>t,Y57>F(- + -)|c195>t|a200>t,Y67>F(- + -)|c204>t|a232>c,I78>L(+ + +)|a251>g,K84>R(+ + +)|a269>g,K90>R(+ + +)|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g303>t|c309>t|c314>t,A105>V(+ - +)| g79>a,G27>N(- - -)|g80>a,G27>N(- - -)|a100>g,S34>G(+ + -)|t102>a,S34>G(+ + -)|a106>g,S36>V(- - -)|g107>t,S36>V(- - -)| g119>c,G40>A(- + -)|g140>a,G47>E(- - -)|g147>t|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|g162>a|t165>c| a170>t,Y57>F(- + -)|c195>t| a200>t,Y67>F(- + -)|c204>t|a232>c,I78>L(+ + +)|a251>g,K84>R(+ + +)|a269>g,K90>R(+ + +)|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g303>t|c309>t| c314>t,A105>V(+ - +)| 320 229 204 25 7 18 4 6 4 1 1 0 2 1 2 1 3 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 0 6 2 2 0 0 0 0 0 1 0 0 1 0 51 51 44 7 3 4 0 3 0 1 0 0 0 0 2 0 1 0 30 21 19 2 1 1 0 0 1 0 0 0 1 0 0 0 0 0 117 114 105 9 3 6 2 1 2 0 1 0 1 0 0 1 1 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct aatggctccatcagcggaagtgtttactac tgggcctggatccgccagcccccagagaagggtctggagtacattggaagc atcttttatagtgggagcact tacttcaatccgtccctcaagagtcgagtcaccctatccgtagacacgtccaggaaccagttctccctgaggctgaagtctgtgaccgccgcagacacggctgtttattattgt gtgagacatgttcgacgattctggttcgacccc VRHVRRFWFDP IGHV4-39 IGHD3-10 IGHJ5 aatggctccatcagcggaagtgtttactac tgggcctggatccgccagcccccagagaagggtctggagtacattggaagc atcttttatagtgggagcact tacttcaatccgtccctcaagagtcgagtcaccctatccgtagacacgtccaggaaccagttctccctgaggctgaagtctgtgaccgccgcagacacggctgtttattattgt gtgagacatgttcgacgattctggttcgacccc IGA1 24 216 14 10 9 15 5 9 6 1 10 7 JY8QFUQ01A365U 8798 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 918 91.71 199/217 nt NA Homsap IGHJ5*02 F 237 96.08 49/51 nt Homsap IGHD6-6*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVRSEYSSSNWFDPW in-frame + ctctggattccccatcagtggctttagaatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattgatagttttagtcagaacatatactacagagactcagtgaggggccgattcaccatctccagagacaacgccaggaactcattgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgtgagatccgagtatagcagttcaaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [287, 288] a85>c,T29>P(+ + -)|t88>a,F30>I(+ - -)|a106>g,S36>G(+ + -)|a110>t,Y37>F(- + -)|c114>a,S38>R(- - -)|a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a175>t,S59>F(- - -)|g176>t,S59>F(- - -)|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>g,S63>Q(- - -)|t190>a,Y64>N(- - -)|g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|a215>g,K72>R(+ + +)|a251>g,K84>R(+ + +)|c259>t|c314>t,A105>V(+ - +)| a85>c,T29>P(+ + -)|t88>a,F30>I(+ - -)|a106>g,S36>G(+ + -)|a110>t,Y37>F(- + -)|c114>a,S38>R(- - -)| a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a175>t,S59>F(- - -)|g176>t,S59>F(- - -)|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>g,S63>Q(- - -)|t190>a,Y64>N(- - -)| g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|a215>g,K72>R(+ + +)|a251>g,K84>R(+ + +)|c259>t| c314>t,A105>V(+ - +)|g319>t|a320>c| 318 (320) 223 (225) 204 19 (21) 1 (3) 18 4 3 2 0 2 (3) 1 2 2 0 1 1 (2) 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 0 0 0 1 1 1 1 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 16 8 0 8 1 2 0 0 1 0 1 1 0 0 1 1 117 114 109 5 1 4 2 1 1 0 0 0 0 0 0 1 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattccccatcagtggctttaga atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attgatagttttagtcagaacata tactacagagactcagtgaggggccgattcaccatctccagagacaacgccaggaactcattgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gtgagatccgagtatagcagttcaaactggttcgacccc VRSEYSSSNWFDP IGHV3-21 IGHD6-6 IGHJ5 ggattccccatcagtggctttaga atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attgatagttttagtcagaacata tactacagagactcagtgaggggccgattcaccatctccagagacaacgccaggaactcattgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gtgagatccgagtatagcagttcaaactggttcgacccc IGA2 18 213 8 10 4 7 4 11 1 0 4 13 JY8QFUQ01A36CM 6677 productive Homsap IGHV3-48*02 F (see comment) 729 81.57 177/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-21*01 F 2 8 8 10 8.8.10 [1.17.38.11] CARSTDGYYDHW in-frame - Low V-REGION identity (81,57% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctggattccccttcaatatatggagcatgaattgggtccgccaggctccggggaagggactggagttgatcgcatacatcacaagtgatgaaagaaccatatactacgcagactctgtgaagggccgcttcaccatctctagagacaatgccaggaacctagtacatctggaaatgaacagcctgagggacggcgatctggctatctattactgcgcgagaagcactgatggctattacgaccactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [279] a85>c,T29>P(+ + -)|g104>a,S35>N(- - -)|g107>t,S36>I(- - -)|c108>a,S36>I(- - -)|a110>g,Y37>W(- + -)|t111>g,Y37>W(- + -)|c120>t|a138>g|g147>a|g155>t,W52>L(+ - -)|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|t160>g,S54>A(- + -)|t168>c|g170>c,S57>T(+ - +)|t171>a,S57>T(+ - +)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|a184>g,S62>E(- - -)|g185>a,S62>E(- - -)|t186>a,S62>E(- - -)|t189>a,S63>R(- - -)|a225>c|c237>t|a251>g,K84>R(+ + +)|t256>c,S86>L(- - -)|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t262>c,Y88>H(+ - -)|c268>g,Q90>E(+ + -)|a285>g|a290>g,E97>G(- - -)|g291>c,E97>G(- - -)|c294>t|a295>c,T99>L(- - -)|c296>t,T99>L(- - -)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)|t312>c| a85>c,T29>P(+ + -)|g104>a,S35>N(- - -)|g107>t,S36>I(- - -)|c108>a,S36>I(- - -)|a110>g,Y37>W(- + -)|t111>g,Y37>W(- + -)| c120>t|a138>g|g147>a|g155>t,W52>L(+ - -)|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|t160>g,S54>A(- + -)| t168>c|g170>c,S57>T(+ - +)|t171>a,S57>T(+ - +)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|a184>g,S62>E(- - -)|g185>a,S62>E(- - -)|t186>a,S62>E(- - -)|t189>a,S63>R(- - -)| a225>c|c237>t|a251>g,K84>R(+ + +)|t256>c,S86>L(- - -)|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t262>c,Y88>H(+ - -)|c268>g,Q90>E(+ + -)|a285>g|a290>g,E97>G(- - -)|g291>c,E97>G(- - -)|c294>t|a295>c,T99>L(- - -)|c296>t,T99>L(- - -)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)|t312>c| g319>a|a320>g| 318 (320) 223 (225) 183 40 (42) 9 (11) 31 7 (8) 7 (8) 5 5 3 1 0 3 3 2 2 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 1 0 0 1 1 0 0 0 0 1 1 51 51 44 7 3 4 1 2 1 1 0 0 0 0 0 0 1 1 30 24 15 9 1 8 2 2 0 1 0 0 0 3 1 0 0 0 117 114 96 18 5 13 3 2 4 3 2 0 0 0 2 2 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ctct ggattccccttcaatatatggagc atgaattgggtccgccaggctccggggaagggactggagttgatcgcatac atcacaagtgatgaaagaaccata tactacgcagactctgtgaagggccgcttcaccatctctagagacaatgccaggaacctagtacatctggaaatgaacagcctgagggacggcgatctggctatctattactgc gcgagaagcactgatggctattacgaccac ARSTDGYYDH IGHV3-48 IGHD2-21 IGHJ4 ggattccccttcaatatatggagc atgaattgggtccgccaggctccggggaagggactggagttgatcgcatac atcacaagtgatgaaagaaccata tactacgcagactctgtgaagggccgcttcaccatctctagagacaatgccaggaacctagtacatctggaaatgaacagcctgagggacggcgatctggctatctattactgc gcgagaagcactgatggctattacgaccac IGG2 40 213 24 16 12 20 12 20 8 1 17 14 JY8QFUQ01A39KY 16074 productive Homsap IGHV1-69*08 F 930 92.99 199/214 nt NA Homsap IGHJ4*03 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 3 8 8 14 8.8.14 [0.17.38.11] CARDSNGYSSGYIDCW in-frame + tggaggctccttcggcagctacactatcacctgggtgcgacaggcccctggacaagggcttgagtggatgggaaggatcacccctatccttggttcaacaagctactcacagaagttccagggcagagtcacgattaccgcggacacattcacgggcacagcctacatggagctgagcagcctgacatctgaagacacggccgtatattactgtgcgcgagactcgaatggatacagttctggttatattgactgctggggccaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtcagggcttcttccc NA IGA1 100 100 [287] a85>t,T29>S(+ - +)|a103>g,S35>G(+ + -)|t111>c|g119>c,S40>T(+ - +)|t170>c,I57>T(- - -)|a190>t,T64>S(+ - +)|g193>a,A65>T(- - -)|a197>g,N66>S(- - -)|g202>t,A68>S(- + -)|a245>c,K82>T(- - -)|c248>t,S83>F(- - -)|a253>g,S85>G(+ + -)|g284>c,R95>T(- - -)|g291>a|g303>a|a316>c| a85>t,T29>S(+ - +)|a103>g,S35>G(+ + -)|t111>c| g119>c,S40>T(+ - +)| t170>c,I57>T(- - -)|a190>t,T64>S(+ - +)|g193>a,A65>T(- - -)| a197>g,N66>S(- - -)|g202>t,A68>S(- + -)|a245>c,K82>T(- - -)|c248>t,S83>F(- - -)|a253>g,S85>G(+ + -)|g284>c,R95>T(- - -)|g291>a|g303>a| a316>c| 320 222 206 16 4 12 3 3 1 2 2 0 2 0 2 0 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 1 0 0 1 0 0 1 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 21 3 0 3 0 1 0 1 0 0 1 0 0 0 0 0 117 114 106 8 2 6 2 2 1 0 1 0 0 0 1 0 1 0 8 8 7 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 aa,17-18(FR1)|aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,162-163(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|tt,185-186(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggca,82-85(CDR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agca,192-195(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,111-114(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggaggctccttcggcagctacact atcacctgggtgcgacaggcccctggacaagggcttgagtggatgggaagg atcacccctatccttggttcaaca agctactcacagaagttccagggcagagtcacgattaccgcggacacattcacgggcacagcctacatggagctgagcagcctgacatctgaagacacggccgtatattactgt gcgcgagactcgaatggatacagttctggttatattgactgc ARDSNGYSSGYIDC IGHV1-69 IGHD5-18 IGHJ4 ggaggctccttcggcagctacact atcacctgggtgcgacaggcccctggacaagggcttgagtggatgggaagg atcacccctatccttggttcaaca agctactcacagaagttccagggcagagtcacgattaccgcggacacattcacgggcacagcctacatggagctgagcagcctgacatctgaagacacggccgtatattactgt gcgcgagactcgaatggatacagttctggttatattgactgc IGA1 15 213 9 6 4 7 5 8 2 1 7 5 JY8QFUQ01A3A5F 19737 productive Homsap IGHV1-8*01 F 840 87.85 188/214 nt NA Homsap IGHJ6*02 F 229 85.48 53/62 nt Homsap IGHD1-26*01 F 3 8 8 14 8.8.14 [0.17.38.11] CARVGGTHYHYGVDVW in-frame + cggatacaccttcaccgattatgagatcaactgggtgcgacaggccactggacaagggcttgagtggatgggaaggataaagcccaatactggttacacagaatatgtacagaagttccagggcagagtcaccctgaccagggacacgtccatgggtacagcctacatggagttgcacagcctgagatctgacgacacggccgtgtattattgcgcgagagtcggtgggacccactaccactatggtgtggacgtctggggccaagggaccacggtcaccgtctcttcagcatccccgtccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [287] t78>c|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t114>g,D38>E(+ - +)|t163>a,W55>R(- - -)|g168>a,M56>I(+ + -)|c171>g,N57>K(+ - -)|t174>c|c177>t|g185>c,S62>T(+ - +)|a190>t,N64>Y(- - -)|g197>a,G66>E(- - -)|c198>a,G66>E(- - -)|c203>t,A68>V(+ - +)|a232>c,M78>L(+ + -)|a241>g,N81>D(+ + -)|c246>g|a252>g,I84>M(+ + -)|a253>g,S85>G(+ + -)|c255>t,S85>G(+ + -)|c271>t|a274>c,S92>H(+ - -)|g275>a,S92>H(+ - -)|g291>c,E97>D(+ - +)|c309>t|t312>c| t78>c| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t114>g,D38>E(+ - +)| t163>a,W55>R(- - -)| g168>a,M56>I(+ + -)|c171>g,N57>K(+ - -)|t174>c|c177>t|g185>c,S62>T(+ - +)|a190>t,N64>Y(- - -)| g197>a,G66>E(- - -)|c198>a,G66>E(- - -)|c203>t,A68>V(+ - +)|a232>c,M78>L(+ + -)|a241>g,N81>D(+ + -)|c246>g|a252>g,I84>M(+ + -)|a253>g,S85>G(+ + -)|c255>t,S85>G(+ + -)|c271>t|a274>c,S92>H(+ - -)|g275>a,S92>H(+ - -)|g291>c,E97>D(+ - +)|c309>t|t312>c| g320>t| 319 (320) 221 (222) 195 26 (27) 7 (8) 19 4 4 5 3 2 1 1 1 2 2 0 (1) 1 78 1 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 1 0 0 0 0 0 0 0 0 0 1 51 51 50 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 30 24 18 6 2 4 0 1 1 1 0 0 1 0 1 1 0 0 117 114 99 15 4 11 3 2 4 1 2 1 0 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 0 0 (1) 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,118-119(FR2)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|aa,241-242(FR3)|ta,251-252(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,251-252(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|agtt,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,118-121(FR2)|aacc,169-172(CDR2)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| c ggatacaccttcaccgattatgag atcaactgggtgcgacaggccactggacaagggcttgagtggatgggaagg ataaagcccaatactggttacaca gaatatgtacagaagttccagggcagagtcaccctgaccagggacacgtccatgggtacagcctacatggagttgcacagcctgagatctgacgacacggccgtgtattattgc gcgagagtcggtgggacccactaccactatggtgtggacgtc ARVGGTHYHYGVDV IGHV1-8 IGHD1-26 IGHJ6 ggatacaccttcaccgattatgag atcaactgggtgcgacaggccactggacaagggcttgagtggatgggaagg ataaagcccaatactggttacaca gaatatgtacagaagttccagggcagagtcaccctgaccagggacacgtccatgggtacagcctacatggagttgcacagcctgagatctgacgacacggccgtgtattattgc gcgagagtcggtgggacccactaccactatggtgtggacgtc IGA1 25 213 15 10 9 14 6 11 4 2 12 7 JY8QFUQ01A3BD5 20429 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 940 93.06 201/216 nt NA Homsap IGHJ5*02 F 183 84.31 43/51 nt Homsap IGHD5-24*01 ORF 1 8 7 12 8.7.12 [2.17.38.11] CARAPRTATLLDPW in-frame - gtctctggcgactccatcagtggtcactactggagctggatcaggcagcccccagggaagggactgcagtggattggttacatctatcacagtgggagcaccaactacaacccctccctcgagagtcgagtctccatttcagtagacacgtccaagaaccagttctccctgaggttgagttctgtgaccgctgcggacacggccgtgtattactgtgcgagagccccgaggacggctacacttctcgacccctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [284] t81>c|g83>a,G28>D(- - -)|a106>g,S36>G(+ + -)|t109>c,Y37>H(+ - -)|c127>a|g151>c,E51>Q(+ + -)|g162>t|t165>c|t172>c,Y58>H(+ - -)|a214>g,K72>E(+ - -)|a229>t,T77>S(+ - +)|a234>t|a269>g,K90>R(+ + +)|c271>t|c276>t| t81>c|g83>a,G28>D(- - -)|a106>g,S36>G(+ + -)|t109>c,Y37>H(+ - -)| c127>a|g151>c,E51>Q(+ + -)|g162>t|t165>c| t172>c,Y58>H(+ - -)| a214>g,K72>E(+ - -)|a229>t,T77>S(+ - +)|a234>t|a269>g,K90>R(+ + +)|c271>t|c276>t| a320>c| 319 (320) 223 (224) 208 15 (16) 7 (8) 8 3 1 2 4 0 (1) 1 2 0 1 0 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 0 2 0 0 0 0 0 0 0 0 51 51 47 4 3 1 0 0 0 1 0 1 0 0 1 0 1 0 30 21 20 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 117 114 108 6 3 3 2 0 2 0 0 0 2 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct ggcgactccatcagtggtcactac tggagctggatcaggcagcccccagggaagggactgcagtggattggttac atctatcacagtgggagcacc aactacaacccctccctcgagagtcgagtctccatttcagtagacacgtccaagaaccagttctccctgaggttgagttctgtgaccgctgcggacacggccgtgtattactgt gcgagagccccgaggacggctacacttctcgacccc ARAPRTATLLDP IGHV4-59 IGHD5-24 IGHJ5 ggcgactccatcagtggtcactac tggagctggatcaggcagcccccagggaagggactgcagtggattggttac atctatcacagtgggagcacc aactacaacccctccctcgagagtcgagtctccatttcagtagacacgtccaagaaccagttctccctgaggttgagttctgtgaccgctgcggacacggccgtgtattactgt gcgagagccccgaggacggctacacttctcgacccc IGG1 15 210 10 5 3 6 7 9 6 1 4 4 JY8QFUQ01A3C67 8032 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 995 95.41 208/218 nt 94.95 207/218 nt Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD2-15*01 F 3 8 8 17 8.8.17 [1.17.38.11] CARDTLEVVSNMDDAFDFW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 41 of V-REGION: 'G' (1 nucleotides at position 37 to 37 in the user submitted sequence), (cause frameshift); cctctggattcaccttcagtcgttatagcatgaactGgggtccgccaggctccagggaaggggctggagtgggtctcatacattagtaggactactactgacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatatttctgtgcgagagatactctagaggtggtgtctaatatggatgatgcttttgatttctggggccaagggacagtggtcaatgtctcctcagcatccccgaccagccccaaggtcttcccggctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 95 [302, 301] a106>c,S36>R(- - -)|c108>t,S36>R(- - -)|c164>a,S55>Y(+ - -)|t174>g,S58>R(- - -)|g176>c,S59>T(+ - +)|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t190>g,Y64>D(- - -)|g303>a|a308>t,Y103>F(- + -)| a106>c,S36>R(- - -)|c108>t,S36>R(- - -)| c164>a,S55>Y(+ - -)| t174>g,S58>R(- - -)|g176>c,S59>T(+ - +)|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t190>g,Y64>D(- - -)| g303>a|a308>t,Y103>F(- + -)| 320 226 216 10 1 9 0 1 1 0 1 1 1 0 3 0 0 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 1 0 1 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 30 24 19 5 0 5 0 0 0 0 0 0 0 0 3 0 0 2 117 114 112 2 1 1 0 1 0 0 0 0 1 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtcgttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatac attagtaggactactactgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatatttctgt gcgagagatactctagaggtggtgtctaatatggatgatgcttttgatttc ARDTLEVVSNMDDAFDF IGHV3-21 IGHD2-15 IGHJ3 ggattcaccttcagtcgttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatac attagtaggactactactgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatatttctgt gcgagagatactctagaggtggtgtctaatatggatgatgcttttgatttc IGA1 10 213 2 8 2 6 0 4 1 0 2 7 JY8QFUQ01A3DG1 12338 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 900 90.78 197/217 nt NA Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD6-13*01 F 2 8 8 16 8.8.16 [1.17.38.11] CARGRIVSVGMSGWFDPW in-frame + ctctggattcacctttagcaactatgccatgagttgggtccgccaggctcaagggaaggggctggactgggtctcagatattagtaatagtggtggtgacacattctacgcaggctccgtgaagggccgcttcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtatattattgtgcaagaggccgtatagtatcagttgggatgtcaggctggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcacgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [296] g107>a,S36>N(- - -)|c120>t|c137>a,P46>Q(- - -)|g153>c,E51>D(+ - +)|c164>a,A55>D(- - -)|g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a197>t,Y66>F(- + -)|a206>g,D69>G(- - -)|g225>c|t246>c|t247>g,S83>A(- + -)|g258>c|c279>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c300>t|c309>t|g315>a|a317>g,K106>R(+ + +)| g107>a,S36>N(- - -)| c120>t|c137>a,P46>Q(- - -)|g153>c,E51>D(+ - +)|c164>a,A55>D(- - -)| g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a197>t,Y66>F(- + -)|a206>g,D69>G(- - -)|g225>c|t246>c|t247>g,S83>A(- + -)|g258>c|c279>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c300>t|c309>t| g315>a|a317>g,K106>R(+ + +)|a320>g| 319 (320) 224 (225) 202 22 (23) 8 (9) 14 3 (4) 5 5 1 0 2 1 0 4 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 47 4 1 3 0 0 1 0 0 2 0 0 1 0 0 0 30 24 20 4 0 4 1 3 0 0 0 0 0 0 0 0 0 0 117 114 103 11 6 5 1 0 4 1 0 0 1 0 3 0 0 1 7 (8) 7 5 2 (3) 1 (2) 1 1 (2) 1 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcaactatgcc atgagttgggtccgccaggctcaagggaaggggctggactgggtctcagat attagtaatagtggtggtgacaca ttctacgcaggctccgtgaagggccgcttcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtatattattgt gcaagaggccgtatagtatcagttgggatgtcaggctggttcgacccc ARGRIVSVGMSGWFDP IGHV3-23 IGHD6-13 IGHJ5 ggattcacctttagcaactatgcc atgagttgggtccgccaggctcaagggaaggggctggactgggtctcagat attagtaatagtggtggtgacaca ttctacgcaggctccgtgaagggccgcttcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtatattattgt gcaagaggccgtatagtatcagttgggatgtcaggctggttcgacccc IGA1 20 213 12 8 9 15 3 5 7 0 8 5 JY8QFUQ01A3EUV 2749 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD5-24*01 ORF 1 8 8 12 8.8.12 [1.17.38.11] CARDRLTISALDYW in-frame + ctctggattcacctttaacaactacgccatgtcctgggtccgccaggctccagggaaggggcttgagtgggtctcagctataactgatagcggtctttacacatactacgcagactccgtgaggggccggttcaccgtctccagagacacttccaagaacacgctgtttctgcaaatggacagcctgagagccgaggacacggccgtatatttctgtgcgagggatagactcacgatttcagcccttgattactggggccagggagccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [285] g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|t111>c|a118>t|g119>c|g150>t|t168>a|g170>c,S57>T(+ - +)|g173>a,G58>D(- - -)|t177>c|g187>c,G63>L(- - -)|g188>t,G63>L(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|a245>c,N82>T(- + -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|a308>t,Y103>F(- + -)| g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|t111>c| a118>t|g119>c|g150>t| t168>a|g170>c,S57>T(+ - +)|g173>a,G58>D(- - -)|t177>c|g187>c,G63>L(- - -)|g188>t,G63>L(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)| a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|a245>c,N82>T(- + -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|a308>t,Y103>F(- + -)| a317>g,K106>R(+ + +)|a318>g,K106>R(+ + +)| 316 (320) 221 (225) 201 (203) 20 (22) 6 14 (16) 3 (5) 4 0 2 1 0 4 1 3 0 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 2 0 1 0 0 0 0 0 0 0 0 51 51 48 3 3 0 0 0 0 0 0 0 1 0 1 0 1 0 30 24 16 8 2 6 0 2 0 1 0 0 1 1 2 0 1 0 117 114 108 6 0 6 3 0 0 0 1 0 2 0 0 0 0 0 4 (8) 4 4 (6) 0 (2) 0 0 (2) 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaacaactacgcc atgtcctgggtccgccaggctccagggaaggggcttgagtgggtctcagct ataactgatagcggtctttacaca tactacgcagactccgtgaggggccggttcaccgtctccagagacacttccaagaacacgctgtttctgcaaatggacagcctgagagccgaggacacggccgtatatttctgt gcgagggatagactcacgatttcagcccttgattac ARDRLTISALDY IGHV3-23 IGHD5-24 IGHJ4 ggattcacctttaacaactacgcc atgtcctgggtccgccaggctccagggaaggggcttgagtgggtctcagct ataactgatagcggtctttacaca tactacgcagactccgtgaggggccggttcaccgtctccagagacacttccaagaacacgctgtttctgcaaatggacagcctgagagccgaggacacggccgtatatttctgt gcgagggatagactcacgatttcagcccttgattac IGG1 20 213 9 11 4 9 5 11 3 3 6 8 JY8QFUQ01A3F5T 6244 productive Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 909 91.24 198/217 nt NA Homsap IGHJ6*02 F (see comment) 194 83.64 46/55 nt Homsap IGHD3-16*01 F 2 8 8 10 8.8.10 [1.17.38.11] CAREVSDALDVW in-frame + Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ctctggattcacctttgatgattttggcatgagatgggtccgccaagtcccagggaaggggctacagtgggtctctgggattaattggaatggtgctaaaacaggttatgcagcctctgtgcagggccgattcaccatctccagagacaacgacaacaacgtcctgtatctgcaaatgaacagtttgagacccgaggacacggccttgtatcgctgtgcgagagaggtttcggacgctctggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcaccccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 79 95 [278, 279] a110>t,Y37>F(- + -)|c120>a,S40>R(- - -)|c134>t,A45>V(+ - +)|t135>c,A45>V(+ - +)|g150>a|g151>c,E51>Q(+ + -)|t165>g|g188>c,G63>A(- + -)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|a206>c,D69>A(- - -)|a214>c,K72>Q(+ - -)|c248>a,A83>D(- - -)|g252>c,K84>N(+ - -)|t256>g,S86>V(- - -)|c257>t,S86>V(- - -)|c280>t|g286>c,A96>P(- - -)|a308>g,H103>R(- - +)| a110>t,Y37>F(- + -)| c120>a,S40>R(- - -)|c134>t,A45>V(+ - +)|t135>c,A45>V(+ - +)|g150>a|g151>c,E51>Q(+ + -)|t165>g| g188>c,G63>A(- + -)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)| a206>c,D69>A(- - -)|a214>c,K72>Q(+ - -)|c248>a,A83>D(- - -)|g252>c,K84>N(+ - -)|t256>g,S86>V(- - -)|c257>t,S86>V(- - -)|c280>t|g286>c,A96>P(- - -)|a308>g,H103>R(- - +)| 320 225 206 19 3 16 1 2 3 1 2 3 1 0 4 0 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 51 51 45 6 2 4 0 1 1 1 0 1 0 0 1 0 0 1 30 24 21 3 0 3 0 1 0 0 0 1 0 0 1 0 0 0 117 114 105 9 1 8 1 0 2 0 2 1 0 0 2 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ctct ggattcacctttgatgattttggc atgagatgggtccgccaagtcccagggaaggggctacagtgggtctctggg attaattggaatggtgctaaaaca ggttatgcagcctctgtgcagggccgattcaccatctccagagacaacgacaacaacgtcctgtatctgcaaatgaacagtttgagacccgaggacacggccttgtatcgctgt gcgagagaggtttcggacgctctggacgtc AREVSDALDV IGHV3-20 IGHD3-16 IGHJ6 ggattcacctttgatgattttggc atgagatgggtccgccaagtcccagggaaggggctacagtgggtctctggg attaattggaatggtgctaaaaca ggttatgcagcctctgtgcagggccgattcaccatctccagagacaacgacaacaacgtcctgtatctgcaaatgaacagtttgagacccgaggacacggccttgtatcgctgt gcgagagaggtttcggacgctctggacgtc IGA1 19 213 7 12 5 12 2 7 3 0 12 4 JY8QFUQ01A3H9O 8334 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 940 93.06 201/216 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD1-26*01 F 1 8 8 12 8.8.12 [1.17.38.11] CAKGIVGDTFFDYW in-frame + tctggattcacctttagtaattatgccctgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggaatcagtagtagtggtgagatcccaaactacgcagactccgtgaagggccggttcaccatctccagagacaattccaggaacacgctgtatctgcaaatgaacagcctgagagtcgaggacacggccgtatattactgtgcgaaagggatagtgggagacactttctttgactactggggccagggagccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [284] c105>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a115>c,M39>L(+ + -)|c164>g,A55>G(- + -)|t165>a,A55>G(- + -)|t168>c|g172>a,G58>S(+ + -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|g191>t,S64>I(- - -)|a193>c,T65>P(+ + -)|t196>a,Y66>N(- - -)|a251>g,K84>R(+ + +)|c287>t,A96>V(+ - +)| c105>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a115>c,M39>L(+ + -)|c164>g,A55>G(- + -)|t165>a,A55>G(- + -)| t168>c|g172>a,G58>S(+ + -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|g191>t,S64>I(- - -)|a193>c,T65>P(+ + -)| t196>a,Y66>N(- - -)|a251>g,K84>R(+ + +)|c287>t,A96>V(+ - +)| a320>g| 319 (320) 223 (224) 208 15 (16) 2 (3) 13 1 (2) 3 3 1 2 0 0 2 0 1 1 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 2 0 0 0 0 0 0 0 0 0 51 51 48 3 0 3 0 0 0 0 1 0 0 1 0 1 0 0 30 24 18 6 1 5 0 2 0 1 1 0 0 0 0 0 1 1 117 114 111 3 0 3 1 0 1 0 0 0 0 1 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcacctttagtaattatgcc ctgagctgggtccgccaggctccagggaaggggctggagtgggtctcagga atcagtagtagtggtgagatccca aactacgcagactccgtgaagggccggttcaccatctccagagacaattccaggaacacgctgtatctgcaaatgaacagcctgagagtcgaggacacggccgtatattactgt gcgaaagggatagtgggagacactttctttgactac AKGIVGDTFFDY IGHV3-23 IGHD1-26 IGHJ4 ggattcacctttagtaattatgcc ctgagctgggtccgccaggctccagggaaggggctggagtgggtctcagga atcagtagtagtggtgagatccca aactacgcagactccgtgaagggccggttcaccatctccagagacaattccaggaacacgctgtatctgcaaatgaacagcctgagagtcgaggacacggccgtatattactgt gcgaaagggatagtgggagacactttctttgactac IGG1 15 213 8 7 6 8 2 7 0 2 6 7 JY8QFUQ01A3HCE 3360 productive Homsap IGHV3-11*01 F 823 87.04 188/216 nt NA Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD6-19*01 F 2 8 8 13 8.8.13 [1.17.38.11] CVREVTGVWDWFDPW in-frame + tctggattcaccttcaaagactactatatgacctggatccgccaagttccagggaaggggctggagcggatctcatatatcagtggcagtggtggcaccatttactacgcagactctgtgaagggccggttcgccatctccagggacaacgccaagaacttactatacttacagatgaacagcctgagagtcgaggacacggccatatatcactgtgtgcgagaagtgactggagtctgggactggtttgacccctggggccggggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [286] g104>a,S35>K(- - -)|t105>a,S35>K(- - -)|c114>t|g119>c,S40>T(+ - +)|g132>a|c134>t,A45>V(+ - +)|t154>c,W52>R(- - -)|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|c165>t|t168>c|a172>g,S58>G(+ + -)|t174>c,S58>G(+ + -)|a187>g,S63>G(+ + -)|t189>c,S63>G(+ + -)|a195>t|a225>g|a229>g,T77>A(- - -)|c257>t,S86>L(- - -)|g261>a|t264>c|c265>t|g267>a|a270>g|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|t307>c,Y103>H(+ - -)|c314>t,A105>V(+ - +)|a316>c| g104>a,S35>K(- - -)|t105>a,S35>K(- - -)|c114>t| g119>c,S40>T(+ - +)|g132>a|c134>t,A45>V(+ - +)|t154>c,W52>R(- - -)|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|c165>t| t168>c|a172>g,S58>G(+ + -)|t174>c,S58>G(+ + -)|a187>g,S63>G(+ + -)|t189>c,S63>G(+ + -)|a195>t| a225>g|a229>g,T77>A(- - -)|c257>t,S86>L(- - -)|g261>a|t264>c|c265>t|g267>a|a270>g|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|t307>c,Y103>H(+ - -)| c314>t,A105>V(+ - +)|a316>c| 320 224 194 30 12 18 5 7 7 7 1 0 1 1 1 0 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 0 0 0 0 1 0 0 0 0 51 51 44 7 2 5 0 2 2 2 0 0 0 0 1 0 0 0 30 24 18 6 2 4 2 0 0 3 0 0 1 0 0 0 0 0 117 114 102 12 6 6 3 4 3 2 0 0 0 0 0 0 0 0 8 8 6 2 1 1 0 0 1 0 1 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcaaagactactat atgacctggatccgccaagttccagggaaggggctggagcggatctcatat atcagtggcagtggtggcaccatt tactacgcagactctgtgaagggccggttcgccatctccagggacaacgccaagaacttactatacttacagatgaacagcctgagagtcgaggacacggccatatatcactgt gtgcgagaagtgactggagtctgggactggtttgacccc VREVTGVWDWFDP IGHV3-11 IGHD6-19 IGHJ5 ggattcaccttcaaagactactat atgacctggatccgccaagttccagggaaggggctggagcggatctcatat atcagtggcagtggtggcaccatt tactacgcagactctgtgaagggccggttcgccatctccagggacaacgccaagaacttactatacttacagatgaacagcctgagagtcgaggacacggccatatatcactgt gtgcgagaagtgactggagtctgggactggtttgacccc IGA1 28 213 25 3 13 14 12 14 8 3 11 6 JY8QFUQ01A3I1N 2452 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 954 93.55 203/217 nt NA Homsap IGHJ6*02 F (see comment) 175 75.81 47/62 nt Homsap IGHD6-13*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARRPAAGSHFALDVW in-frame - Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) or Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ttctggatacaactttgacaccgattggatcgcctgggtgcgccagatgcccgggaaaggcctggagtggatggggatcatctatcctgctgactctgataccagatacagtccgtccttccaaggccaagtcaccatctcagccgacaagtccatcaacaccgcctacctgcagtggagcggcctgaaggcctcggacaccgccatctattattgtgcgaggcgtccagcagctggtagccacttcgctttggacgtctggggccaggggaccacggtcaccgtctcctcagcatcccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccca NA IGA2 79 89 [289, 288] g86>a,S29>N(- - -)|a103>g,T35>D(- + -)|c104>a,T35>D(- + -)|g107>c,S36>T(+ - +)|t109>g,Y37>D(- - -)|c111>t,Y37>D(- - -)|g119>c,G40>A(- + -)|g176>c,G59>A(- + -)|c204>t|g225>a|g254>a,S85>N(- - -)|a277>g,S93>G(+ + -)|g303>c,M101>I(+ + -)|c309>t| g86>a,S29>N(- - -)|a103>g,T35>D(- + -)|c104>a,T35>D(- + -)|g107>c,S36>T(+ - +)|t109>g,Y37>D(- - -)|c111>t,Y37>D(- - -)| g119>c,G40>A(- + -)| g176>c,G59>A(- + -)| c204>t|g225>a|g254>a,S85>N(- - -)|a277>g,S93>G(+ + -)|g303>c,M101>I(+ + -)|c309>t| a318>g|a320>g| 317 (320) 222 (225) 208 (209) 14 (16) 3 (5) 11 2 (4) 3 3 0 0 1 0 0 4 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 1 1 0 0 1 0 0 1 0 0 1 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 117 114 108 6 3 3 1 2 2 0 0 0 0 0 1 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ttct ggatacaactttgacaccgattgg atcgcctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctgctgactctgatacc agatacagtccgtccttccaaggccaagtcaccatctcagccgacaagtccatcaacaccgcctacctgcagtggagcggcctgaaggcctcggacaccgccatctattattgt gcgaggcgtccagcagctggtagccacttcgctttggacgtc ARRPAAGSHFALDV IGHV5-51 IGHD6-13 IGHJ6 ggatacaactttgacaccgattgg atcgcctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctgctgactctgatacc agatacagtccgtccttccaaggccaagtcaccatctcagccgacaagtccatcaacaccgcctacctgcagtggagcggcctgaaggcctcggacaccgccatctattattgt gcgaggcgtccagcagctggtagccacttcgctttggacgtc IGA2 14 213 8 6 6 11 2 3 3 0 4 7 JY8QFUQ01A3I5W 6691 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 999 95.85 208/217 nt NA Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [1.17.38.11] CAKDGARGGGSCFNSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcacctttagaagctatgccatgagctgggtccgccaggttccagggaaggggctggagtgggtctcagctattagtggtaatggtgctaacacatactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgtgcgaaagatggcgctagaggtggcggcagttgctttaactcctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [291] c105>a,S35>R(- - -)|c134>t,A45>V(+ - +)|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|g191>a,S64>N(- - -)|g225>a|c248>t,S83>F(- - -)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)| c105>a,S35>R(- - -)| c134>t,A45>V(+ - +)| g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|g191>a,S64>N(- - -)| g225>a|c248>t,S83>F(- - -)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)| 320 225 216 9 1 8 0 3 2 0 1 1 0 0 2 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 2 0 0 0 0 0 0 1 0 0 0 117 114 110 4 1 3 0 1 1 0 1 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagaagctatgcc atgagctgggtccgccaggttccagggaaggggctggagtgggtctcagct attagtggtaatggtgctaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtggcggcagttgctttaactcc AKDGARGGGSCFNS IGHV3-23 IGHD5-24 IGHJ4 ggattcacctttagaagctatgcc atgagctgggtccgccaggttccagggaaggggctggagtgggtctcagct attagtggtaatggtgctaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtggcggcagttgctttaactcc IGG2 9 213 5 4 5 8 0 1 1 0 4 4 JY8QFUQ01A3IIQ 14140 productive (see comment) Homsap IGHV3-7*01 F 955 93.15 204/219 nt 92.69 203/219 nt Homsap IGHJ5*02 F (see comment) 183 84.31 43/51 nt Homsap IGHD3-22*01 F 2 8 8 11 8.8.11 [2.17.38.11] CAISSDFYSTGHW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in CDR2-IMGT at codon 57 of V-REGION: 'A' (1 nucleotides at position 86 to 86 in the user submitted sequence), (cause frameshift); ggctctggattcaacttcagaacctattggatgggctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaAagcacgatggaagtgacaagtactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcattgtttttgcaaatggacagcctgagagccgaggacacggctgtgtactactgtgcgattagcagtgatttctactcgacgggccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] c74>g,A25>G(- + -)|c86>a,T29>N(- + -)|t90>c|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|a118>g,S40>G(+ + -)|a174>c,Q58>H(- + -)|g192>c,E64>D(+ - +)|a195>g|a232>g,I78>V(+ - +)|c259>t|a263>t,Y88>F(- + -)|c265>t|a274>g,N92>D(+ + -)|t306>c| c74>g,A25>G(- + -)| c86>a,T29>N(- + -)|t90>c|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)| a118>g,S40>G(+ + -)| a174>c,Q58>H(- + -)|g192>c,E64>D(+ - +)|a195>g| a232>g,I78>V(+ - +)|c259>t|a263>t,Y88>F(- + -)|c265>t|a274>g,N92>D(+ + -)|t306>c| g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 316 (320) 223 (227) 208 15 (19) 5 (7) 10 (12) 4 (5) 0 (1) 2 2 1 1 1 (2) 1 2 1 0 (1) 0 78 6 5 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 36 24 20 4 1 3 0 0 0 1 0 1 0 1 1 0 0 0 51 51 50 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 1 0 0 0 1 0 0 0 1 0 0 0 117 114 108 6 3 3 2 0 2 1 0 0 1 0 0 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ggctct ggattcaacttcagaacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcacgatggaagtgacaag tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcattgtttttgcaaatggacagcctgagagccgaggacacggctgtgtactactgt gcgattagcagtgatttctactcgacgggccac AISSDFYSTGH IGHV3-7 IGHD3-22 IGHJ5 ggattcaacttcagaacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcacgatggaagtgacaag tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcattgtttttgcaaatggacagcctgagagccgaggacacggctgtgtactactgt gcgattagcagtgatttctactcgacgggccac IGA1 14 213 8 6 2 5 6 9 3 2 4 5 JY8QFUQ01A3IMB 6861 productive Homsap IGHV3-7*01 F 918 91.71 199/217 nt NA Homsap IGHJ6*02 F 175 75.81 47/62 nt Homsap IGHD3-16*01 F 1 8 8 14 8.8.14 [1.17.38.11] CARAVGQRGYDGTDLW in-frame + ctctggattcatgttcagcagttattggatgagctgggtccgccaggatccagggaaggggctggagtgggtggccaatataaacgaagaaggaagtgagaaatattatgtggactctgggaagggccgattcaggatctccagagacaacgccaagaattccgtgtatctgcaaatggacagcctgagagccgaggacacggctgtgtattactgtgcgagagcagtggggcagcgaggctacgacggtacagacctctggggccaagggaccacggtcatcgtctccacagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [291] c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|t90>c|t105>c|c108>t|c134>a,A45>D(- - -)|c165>t|g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|t177>a,D59>E(+ - +)|c198>t|t212>g,V71>G(- - -)|c230>g,T77>R(- - -)|c231>g,T77>R(- - -)|c255>t|a258>c|c259>g,L87>V(+ - +)|a274>g,N92>D(+ + -)| c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|t90>c|t105>c|c108>t| c134>a,A45>D(- - -)|c165>t| g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|t177>a,D59>E(+ - +)| c198>t|t212>g,V71>G(- - -)|c230>g,T77>R(- - -)|c231>g,T77>R(- - -)|c255>t|a258>c|c259>g,L87>V(+ - +)|a274>g,N92>D(+ + -)| a320>c| 319 (320) 224 (225) 206 18 (19) 7 (8) 11 1 0 5 2 1 (2) 1 0 1 1 5 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 3 2 0 0 2 2 0 0 0 0 0 1 0 0 51 51 49 2 1 1 0 0 1 0 0 1 0 0 0 0 0 0 30 24 21 3 0 3 0 0 0 0 0 0 0 1 1 1 0 0 117 114 106 8 3 5 1 0 2 0 1 0 0 0 0 3 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatgttcagcagttattgg atgagctgggtccgccaggatccagggaaggggctggagtgggtggccaat ataaacgaagaaggaagtgagaaa tattatgtggactctgggaagggccgattcaggatctccagagacaacgccaagaattccgtgtatctgcaaatggacagcctgagagccgaggacacggctgtgtattactgt gcgagagcagtggggcagcgaggctacgacggtacagacctc ARAVGQRGYDGTDL IGHV3-7 IGHD3-16 IGHJ6 ggattcatgttcagcagttattgg atgagctgggtccgccaggatccagggaaggggctggagtgggtggccaat ataaacgaagaaggaagtgagaaa tattatgtggactctgggaagggccgattcaggatctccagagacaacgccaagaattccgtgtatctgcaaatggacagcctgagagccgaggacacggctgtgtattactgt gcgagagcagtggggcagcgaggctacgacggtacagacctc IGG1 18 213 8 10 5 12 3 6 4 3 6 5 JY8QFUQ01A3IWM 13203 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 979 95.77 204/213 nt 95.31 203/213 nt Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [0.17.37.11] CAKDGARGGGSCFNSW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR3-IMGT, from codon 91 of V-REGION: 1 nucleotides (from position 173 in the user submitted sequence), (cause frameshift) tggattcacctttagaagctatgccatgagctgggtccgccaggttccagggaaggggctggagtgggtctcagctattagtggtaatggtgctaacacatactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaatgaacagcctgagagccgaggacacggccctatattactgtgcgaaagatggcgctagaggtggcggcagttgctttaactcctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [287] c105>a,S35>R(- - -)|c134>t,A45>V(+ - +)|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|g191>a,S64>N(- - -)|g225>a|c248>t,S83>F(- - -)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)| c105>a,S35>R(- - -)| c134>t,A45>V(+ - +)| g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|g191>a,S64>N(- - -)| g225>a|c248>t,S83>F(- - -)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)| 320 221 212 9 1 8 0 3 2 0 1 1 0 0 2 0 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 2 0 0 0 0 0 0 1 0 0 0 117 113 109 4 1 3 0 1 1 0 1 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t ggattcacctttagaagctatgcc atgagctgggtccgccaggttccagggaaggggctggagtgggtctcagct attagtggtaatggtgctaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtggcggcagttgctttaactcc AKDGARGGGSCFNS IGHV3-23 IGHD5-24 IGHJ4 ggattcacctttagaagctatgcc atgagctgggtccgccaggttccagggaaggggctggagtgggtctcagct attagtggtaatggtgctaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtggcggcagttgctttaactcc IGG2 9 212 5 4 5 8 0 1 1 0 4 4 JY8QFUQ01A3KYL 8636 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 981 94.93 206/217 nt 94.47 205/217 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD1-26*01 F 1 8 8 9 8.8.9 [1.17.38.11] CTREVGATDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'G' (1 nucleotides at position 8 to 8 in the user submitted sequence), (cause frameshift); ctctggaGttcaccttcagtagctactggatgcattgggtccgccaagctccagggaaggggctggagtgggtctcacgtattcatagtgatgggactaccacatactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaggaacacgttgtatctgcaattgaacagtctgagagccgaggacacggctgtgtattattgtacaagagaggtgggagctaccgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [276, 275] c120>t|t152>a,V51>E(- + -)|a169>c,N57>H(- - -)|g188>c,S63>T(+ - +)|g191>c,S64>T(+ - +)|a196>t,S66>Y(+ - -)|g197>a,S66>Y(+ - -)|a251>g,K84>R(+ + +)|c259>t|a271>t,M91>L(+ + -)|c309>t|g313>a,A105>T(- - -)| c120>t|t152>a,V51>E(- + -)| a169>c,N57>H(- - -)|g188>c,S63>T(+ - +)|g191>c,S64>T(+ - +)| a196>t,S66>Y(+ - -)|g197>a,S66>Y(+ - -)|a251>g,K84>R(+ + +)|c259>t|a271>t,M91>L(+ + -)|c309>t| g313>a,A105>T(- - -)| 320 225 213 12 3 9 1 2 3 0 1 0 2 1 2 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 0 1 0 0 0 0 1 0 0 0 0 30 24 21 3 0 3 0 0 0 0 1 0 0 0 2 0 0 0 117 114 108 6 2 4 1 1 2 0 0 0 2 0 0 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcattgggtccgccaagctccagggaaggggctggagtgggtctcacgt attcatagtgatgggactaccaca tactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaggaacacgttgtatctgcaattgaacagtctgagagccgaggacacggctgtgtattattgt acaagagaggtgggagctaccgactac TREVGATDY IGHV3-74 IGHD1-26 IGHJ4 ggattcaccttcagtagctactgg atgcattgggtccgccaagctccagggaaggggctggagtgggtctcacgt attcatagtgatgggactaccaca tactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaggaacacgttgtatctgcaattgaacagtctgagagccgaggacacggctgtgtattattgt acaagagaggtgggagctaccgactac IGA2 11 213 5 6 4 6 1 5 3 0 5 3 JY8QFUQ01A3MV4 15355 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-8*01 F 2 8 8 8 8.8.8 [1.17.38.11] CARDNYGDYW in-frame + ctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgtgttaatggtgatgggggtaggacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcacagtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [272, 271] g153>t|a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g153>t| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 225 212 13 5 8 4 2 0 0 0 0 0 1 3 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 30 24 20 4 0 4 3 0 0 0 0 0 0 0 0 1 0 0 117 114 106 8 4 4 1 2 0 0 0 0 0 1 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgt gttaatggtgatgggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgt gttaatggtgatgggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac IGA1 13 213 6 7 2 8 4 5 5 0 4 4 JY8QFUQ01A3NLN 7840 productive (see comment) Homsap IGHV3-7*01 F 994 95.83 207/216 nt 95.37 206/216 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-15*01 F 2 7 8 11 7.8.11 [1.17.38.11] CARSGSGSHLDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 37 of V-REGION: 1 nucleotides (from position 24 in the user submitted sequence), (cause frameshift) ctctggattcacctttagtaggtttggatgacctgggtccgccagggtccagggaaggggctggagtgggtggccaacataaagcaagttggaaatgagagatactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcattgtatctgcaaatgaacagcctgagagtcgacgacacggctgtgtattactgtgcgagaagtggtagtgggagccacttagattactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [280] c108>g,S36>R(- - -)|g119>c,S40>T(+ - +)|c134>g,A45>G(- + -)|a176>t,D59>V(- - -)|g188>a,S63>N(- - -)|a194>g,K65>R(+ + +)|c259>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| c108>g,S36>R(- - -)| g119>c,S40>T(+ - +)|c134>g,A45>G(- + -)| a176>t,D59>V(- - -)|g188>a,S63>N(- - -)|a194>g,K65>R(+ + +)| c259>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| g319>a|a320>g| 318 (320) 222 (224) 213 9 (11) 1 (3) 8 1 (2) 1 (2) 2 0 0 0 1 0 2 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 22 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 49 2 0 2 0 0 0 0 0 0 0 0 1 1 0 0 30 24 21 3 0 3 1 1 0 0 0 0 1 0 0 0 0 0 117 114 111 3 1 2 0 0 2 0 0 0 0 0 1 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtaggtttgg atgacctgggtccgccagggtccagggaaggggctggagtgggtggccaac ataaagcaagttggaaatgagaga tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcattgtatctgcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaagtggtagtgggagccacttagattac ARSGSGSHLDY IGHV3-7 IGHD2-15 IGHJ4 ggattcacctttagtaggtttgg atgacctgggtccgccagggtccagggaaggggctggagtgggtggccaac ataaagcaagttggaaatgagaga tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcattgtatctgcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaagtggtagtgggagccacttagattac IGA1 9 212 4 5 3 7 1 2 1 0 4 4 JY8QFUQ01A3OS9 7020 productive Homsap IGHV4-39*02 F 834 86.82 191/220 nt NA Homsap IGHJ4*02 F (see comment) 168 83.33 40/48 nt Homsap IGHD5-12*01 F 3 10 7 19 10.7.19 [1.17.38.11] CARHVVLDNRYSVWPRILDSW in-frame - Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggtgaccccatcggcaacactgcttactcctggggctggatccgccagcccccagggaaggggctggagtggatcgcgactgtacattatgctggcagcacctactacaacccgtccctcaggagtcgagtcaccatctctgtggacacgtccaagaatcacttctccctgaagctgaattctgtgaccgcctcagacacggctgtatacttctgtgcgagacatgtggttttggacaatagatacagtgtctggcctcgtatccttgattcctggggccagggaacccttgtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttc NA IGA1 100 100 [308] g83>a,G28>D(- - -)|t85>c,S29>P(+ - -)|a91>g,S31>G(+ + -)|g101>a,S34>N(- - -)|t102>c,S34>N(- - -)|g104>c,S35>T(+ - +)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a113>c,Y38>S(+ - -)|t159>c|g161>c,G54>A(- + -)|g164>c,S55>T(+ - +)|a166>g,I56>V(+ - +)|c168>a,I56>V(+ - +)|t169>c,Y57>H(+ - -)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|g189>c|a215>g,K72>R(+ + +)|a234>c|c237>t|a240>g|c255>t|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)|g289>t,A97>S(- + -)|g303>a|t306>c|a308>t,Y103>F(- + -)| g83>a,G28>D(- - -)|t85>c,S29>P(+ - -)|a91>g,S31>G(+ + -)|g101>a,S34>N(- - -)|t102>c,S34>N(- - -)|g104>c,S35>T(+ - +)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a113>c,Y38>S(+ - -)| t159>c|g161>c,G54>A(- + -)|g164>c,S55>T(+ - +)| a166>g,I56>V(+ - +)|c168>a,I56>V(+ - +)|t169>c,Y57>H(+ - -)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|g189>c| a215>g,K72>R(+ + +)|a234>c|c237>t|a240>g|c255>t|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)|g289>t,A97>S(- + -)|g303>a|t306>c|a308>t,Y103>F(- + -)| g319>c| 318 (320) 226 (228) 197 (198) 29 (30) 8 (9) 21 6 4 3 5 2 1 1 0 6 (7) 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 21 9 0 9 2 2 0 2 1 0 0 0 2 0 0 0 51 51 48 3 1 2 0 0 0 1 0 0 0 0 2 0 0 0 30 21 15 6 1 5 2 0 0 1 0 1 0 0 2 0 0 0 117 114 103 11 6 5 2 2 3 1 1 0 1 0 0 0 1 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtgaccccatcggcaacactgcttactcc tggggctggatccgccagcccccagggaaggggctggagtggatcgcgact gtacattatgctggcagcacc tactacaacccgtccctcaggagtcgagtcaccatctctgtggacacgtccaagaatcacttctccctgaagctgaattctgtgaccgcctcagacacggctgtatacttctgt gcgagacatgtggttttggacaatagatacagtgtctggcctcgtatccttgattcc ARHVVLDNRYSVWPRILDS IGHV4-39 IGHD5-12 IGHJ4 ggtgaccccatcggcaacactgcttactcc tggggctggatccgccagcccccagggaaggggctggagtggatcgcgact gtacattatgctggcagcacc tactacaacccgtccctcaggagtcgagtcaccatctctgtggacacgtccaagaatcacttctccctgaagctgaattctgtgaccgcctcagacacggctgtatacttctgt gcgagacatgtggttttggacaatagatacagtgtctggcctcgtatccttgattcc IGA1 29 216 18 11 7 15 11 14 7 1 7 14 JY8QFUQ01A3QD4 19118 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 1052 99.07 213/215 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-10*01 F 2 8 7 11 8.7.11 [1.17.38.11] CARLGPGSGTDYW in-frame + tctctggtggctccatcagtagttcctactggagctggatccggcagcccccagggaagggactggagtggattgggtatatctattacagtgggagcaccaactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgacctctgtgaccgctgcggacacggccgtgtattactgtgcgagactggggcccggttcggggactgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [280] a110>c,Y37>S(+ - -)|g275>c,S92>T(+ - +)| a110>c,Y37>S(+ - -)| g275>c,S92>T(+ - +)| g319>c|a320>t| 318 (320) 221 (223) 219 2 (4) 0 (2) 2 0 0 0 0 1 0 0 (1) 0 1 (2) 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 113 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagtagttcctac tggagctggatccggcagcccccagggaagggactggagtggattgggtat atctattacagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgacctctgtgaccgctgcggacacggccgtgtattactgt gcgagactggggcccggttcggggactgactac ARLGPGSGTDY IGHV4-59 IGHD3-10 IGHJ4 ggtggctccatcagtagttcctac tggagctggatccggcagcccccagggaagggactggagtggattgggtat atctattacagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgacctctgtgaccgctgcggacacggccgtgtattactgt gcgagactggggcccggttcggggactgactac IGG1 2 210 0 2 0 1 0 1 0 0 1 1 JY8QFUQ01A3QEB 7363 productive Homsap IGHV3-9*01 F 927 92.17 200/217 nt NA Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD1-26*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVKGAGTTVNWFDIW in-frame + ctctggattcacctttgatggttatgccatgcactgggtccggcaagctccaggggagggcctggaatgggtctcaagtattaactggaatagtgatacaatagactacgcggactctgtgaagggccgattcactatctccagagacaacgccaagcactccctgtatctacaaatgaacaatctgagaaatgaggacacggccttgtattactgtgtaaaaggagcgggaactaccgtcaactggttcgacatctggggccagggaaccctggtcaccgtctctttagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] a107>g,D36>G(- - -)|a142>g,K48>E(+ - -)|g153>a|g163>a,G55>S(+ + -)|g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)|g197>a,G66>D(- - -)|t201>c|c231>t|a253>c,N85>H(- - -)|g267>a|g278>a,S93>N(- - -)|g286>a,A96>N(- - -)|c287>a,A96>N(- - -)|c314>t,A105>V(+ - +)| a107>g,D36>G(- - -)| a142>g,K48>E(+ - -)|g153>a|g163>a,G55>S(+ + -)| g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)| g197>a,G66>D(- - -)|t201>c|c231>t|a253>c,N85>H(- - -)|g267>a|g278>a,S93>N(- - -)|g286>a,A96>N(- - -)|c287>a,A96>N(- - -)| c314>t,A105>V(+ - +)|a320>g,D107>G(- - -)|t321>a,D107>G(- - -)|a322>g| 319 (322) 224 (227) 206 18 (21) 4 (5) 14 (16) 2 (4) 8 2 2 1 2 0 0 (1) 1 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 51 48 3 1 2 1 2 0 0 0 0 0 0 0 0 0 0 30 24 19 5 0 5 0 2 0 1 0 1 0 0 1 0 0 0 117 114 106 8 3 5 0 4 1 1 1 1 0 0 0 0 0 0 7 (10) 7 6 1 (4) 0 (1) 1 (3) 0 (2) 0 1 0 0 0 0 0 (1) 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttgatggttatgcc atgcactgggtccggcaagctccaggggagggcctggaatgggtctcaagt attaactggaatagtgatacaata gactacgcggactctgtgaagggccgattcactatctccagagacaacgccaagcactccctgtatctacaaatgaacaatctgagaaatgaggacacggccttgtattactgt gtaaaaggagcgggaactaccgtcaactggttcgacatc VKGAGTTVNWFDI IGHV3-9 IGHD1-26 IGHJ5 ggattcacctttgatggttatgcc atgcactgggtccggcaagctccaggggagggcctggaatgggtctcaagt attaactggaatagtgatacaata gactacgcggactctgtgaagggccgattcactatctccagagacaacgccaagcactccctgtatctacaaatgaacaatctgagaaatgaggacacggccttgtattactgt gtaaaaggagcgggaactaccgtcaactggttcgacatc IGA1 17 213 13 4 9 12 4 5 4 0 7 6 JY8QFUQ01A3QNJ 18259 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 823 87.04 188/216 nt 85.65 185/216 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-21*02 F 3 8 7 15 8.7.15 [2.17.38.11] CARGRTVVTVIDYIDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'A' (1 nucleotides at position 49 to 49 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 59 of V-REGION: 'C' (1 nucleotides at position 94 to 94 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 65 of V-REGION: 'C' (1 nucleotides at position 103 to 103 in the user submitted sequence), (cause frameshift); gtctctggggactccattagtggttactattggacgtggatccggcagAgccccagggaagggactggagtggattggaaatgtccattacacCtgggagtaCccaagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgtgcgagaggacgaacggtggtgactgttattgactacattgactattggggtcagggagccctggtcagcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [295] t81>g|g83>a,G28>D(- - -)|c90>t|a106>g,S36>G(+ + -)|c114>t|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>g,P45>A(- - -)|g162>a|t163>a,Y55>N(- - -)|a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|c192>t|c198>g,N66>K(+ - -)|a203>g,N68>S(- - -)|a234>g,I78>M(+ + -)|a240>t|c245>t,T82>M(- - -)|a251>g,K84>R(+ + +)|t262>a,S88>T(+ - +)|g267>c|g270>a|c271>t|g275>c,S92>T(+ - +)|g282>a|a283>g,T95>A(- - -)|g303>c| t81>g|g83>a,G28>D(- - -)|c90>t|a106>g,S36>G(+ + -)|c114>t| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>g,P45>A(- - -)|g162>a|t163>a,Y55>N(- - -)| a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|c192>t| c198>g,N66>K(+ - -)|a203>g,N68>S(- - -)|a234>g,I78>M(+ + -)|a240>t|c245>t,T82>M(- - -)|a251>g,K84>R(+ + +)|t262>a,S88>T(+ - +)|g267>c|g270>a|c271>t|g275>c,S92>T(+ - +)|g282>a|a283>g,T95>A(- - -)|g303>c| a320>g| 319 (320) 223 (224) 195 28 (29) 11 (12) 17 6 (7) 4 5 1 0 0 1 2 5 3 0 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 3 2 1 1 2 0 0 0 0 0 0 0 0 1 51 51 46 5 1 4 0 1 0 0 0 0 0 1 1 2 0 0 30 21 17 4 1 3 1 0 1 1 0 0 0 0 1 0 0 0 117 114 100 14 6 8 4 2 2 0 0 0 1 1 3 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct ggggactccattagtggttactat tggacgtggatccggcaggccccagggaagggactggagtggattggaaat gtccattacactgggagtacc aagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttattgactacattgactat ARGRTVVTVIDYIDY IGHV4-59 IGHD2-21 IGHJ4 ggggactccattagtggttactat tggacgtggatccggcaggccccagggaagggactggagtggattggaaat gtccattacactgggagtacc aagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttattgactacattgactat IGA1 28 210 16 12 9 17 7 11 7 4 12 5 JY8QFUQ01A3R6Q 14253 productive Homsap IGHV6-1*01 F, or Homsap IGHV6-1*02 F 1049 96.04 218/227 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD1-26*01 F 3 10 9 19 10.9.19 [1.17.38.11] CTRGSSRGGRYDENYYELDFW in-frame + ctccgggggacagtgtctctaccaacagagctgcttggaactggatcaggcagtccccatcgagaggccttgagtggctgggaaggacatactacaggtccaagtggtataatgattatgcagtgtctgtgaaaagtcgaataaccatcaacccagacacatccaagaaccagttctccctgcagttgaattctgtgactcccgaggacacggctgtgtattactgtacaagagggtccagccgcggggggagatatgatgagaactactatgagctggacttctggggccagggaaccctggtcaccgtctcctcagcatcccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccc NA IGA2 79 89 [316, 317] t74>c|c75>t|t76>c,S26>P(+ - -)|c78>g,S26>P(+ - -)|g101>c,S34>T(+ - +)|t108>a,S36>R(- - -)|a207>g|c271>t|c276>t|g313>a,A105>T(- - -)| t74>c|c75>t|t76>c,S26>P(+ - -)|c78>g,S26>P(+ - -)| g101>c,S34>T(+ - +)|t108>a,S36>R(- - -)| a207>g|c271>t|c276>t| g313>a,A105>T(- - -)|a320>g| 319 (320) 234 (235) 224 10 (11) 5 (6) 5 1 (2) 1 3 2 0 0 0 1 1 1 0 0 78 5 1 4 2 2 0 0 1 2 0 0 0 0 0 1 0 0 36 30 28 2 0 2 0 0 0 0 0 0 0 1 1 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 27 27 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 111 3 3 0 1 0 2 0 0 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 1 0 0 0 0 0 0 0 0 0 0 ta,5-6(FR1)|aa,40-41(FR1)|aa,103-104(CDR1)|aa,118-119(FR2)|aa,162-163(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,184-185(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|ta,206-207(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,227-228(FR3)|aa,235-236(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,5-6(FR1)|tt,114-115(CDR1)|tt,149-150(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|tt,198-199(FR3)|ta,206-207(FR3)|ta,227-228(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggta,3-6(FR1)|agct,8-11(FR1)|agca,14-17(FR1)|agca,100-103(CDR1)|ggca,128-131(FR2)|ggct,155-158(FR2)|ggta,188-191(CDR2)|agta,204-207(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tgcc,69-72(FR1)|tgct,108-111(CDR1)|aact,118-121(FR2)|tact,169-172(CDR2)|aacc,228-231(FR3)|aacc,235-238(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|aact,274-277(FR3)|tact,307-310(FR3)| ctccg ggggacagtgtctctaccaacagagctgct tggaactggatcaggcagtccccatcgagaggccttgagtggctgggaagg acatactacaggtccaagtggtataat gattatgcagtgtctgtgaaaagtcgaataaccatcaacccagacacatccaagaaccagttctccctgcagttgaattctgtgactcccgaggacacggctgtgtattactgt acaagagggtccagccgcggggggagatatgatgagaactactatgagctggacttc TRGSSRGGRYDENYYELDF IGHV6-1 IGHD1-26 IGHJ4 ggggacagtgtctctaccaacagagctgct tggaactggatcaggcagtccccatcgagaggccttgagtggctgggaagg acatactacaggtccaagtggtataat gattatgcagtgtctgtgaaaagtcgaataaccatcaacccagacacatccaagaaccagttctccctgcagttgaattctgtgactcccgaggacacggctgtgtattactgt acaagagggtccagccgcggggggagatatgatgagaactactatgagctggacttc IGA2 5 222 3 2 2 3 1 2 3 0 0 2 JY8QFUQ01A3SN3 7740 productive (see comment) Homsap IGHV3-7*01 F 954 93.55 203/217 nt 93.09 202/217 nt Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 165 80.39 41/51 nt Homsap IGHD3-22*01 F 2 8 8 13 8.8.13 [1.17.38.11] CATSPSDFDRRWGYW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in CDR2-IMGT at codon 57 of V-REGION: 'A' (1 nucleotides at position 84 to 84 in the user submitted sequence), (cause frameshift); ctctggatttagctttagaaccttttggatgagctgggtccgccaggctccagggagggggctggagtgggtggccaacataaAagtcagatggaagtgacaaatggtatgtggactctgtgaagggccgattcaccatctccagagacaacgcgaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgtgcgacctcccctagcgactttgacagacgatgggggtactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [288, 287] c84>t|c86>g,T29>S(+ - +)|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|a143>g,K48>R(+ + +)|c172>t,Q58>S(- - -)|a173>c,Q58>S(- - -)|g192>c,E64>D(+ - +)|a197>g,Y66>W(- + -)|c198>g,Y66>W(- + -)|c249>g|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| c84>t|c86>g,T29>S(+ - +)|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)| a143>g,K48>R(+ + +)| c172>t,Q58>S(- - -)|a173>c,Q58>S(- - -)|g192>c,E64>D(+ - +)| a197>g,Y66>W(- + -)|c198>g,Y66>W(- + -)|c249>g|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g317>c,R106>T(- - -)|a318>c,R106>T(- - -)|g319>t|a320>c| 316 (320) 221 (225) 207 14 (18) 2 (4) 12 (14) 2 2 2 0 1 (3) 0 1 1 2 (3) 3 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 0 1 0 0 0 1 1 1 1 0 0 51 51 50 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 0 1 0 1 0 0 0 1 0 0 0 117 114 109 5 1 4 1 2 0 0 0 0 0 0 0 2 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 0 0 0 0 (2) 0 0 0 0 (1) 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatttagctttagaaccttttgg atgagctgggtccgccaggctccagggagggggctggagtgggtggccaac ataaagtcagatggaagtgacaaa tggtatgtggactctgtgaagggccgattcaccatctccagagacaacgcgaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgt gcgacctcccctagcgactttgacagacgatgggggtac ATSPSDFDRRWGY IGHV3-7 IGHD3-22 IGHJ5 ggatttagctttagaaccttttgg atgagctgggtccgccaggctccagggagggggctggagtgggtggccaac ataaagtcagatggaagtgacaaa tggtatgtggactctgtgaagggccgattcaccatctccagagacaacgcgaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgt gcgacctcccctagcgactttgacagacgatgggggtac IGA2 14 213 6 8 4 9 2 5 1 1 5 7 JY8QFUQ01A3V0Z 4849 productive Homsap IGHV3-7*01 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD6-19*01 F 1 8 8 10 8.8.10 [1.17.38.11] CAGGRGWLIDYW in-frame + ctctggtttcagcttaagtgactattggatgaactgggtccgccaggctccagggaaggggctcgagtgggtggccatcataaagaaagatggaagtgaagaactctatttggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcactgtatctggaaatgaacagcctgagccccgaggacacggctgtatatttctgtgcggggggcagaggctggcttattgactactggggcctgggaaccgcggtcatcgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [279] a81>t|c86>g,T29>S(+ - +)|t90>a,F30>L(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g119>a,S40>N(- - -)|g150>c|a164>t,N55>I(- - -)|c172>a,Q58>K(+ - -)|g192>a|a193>g,K65>E(+ - -)|t196>c,Y66>L(- - -)|a197>t,Y66>L(- - -)|g202>t,V68>L(+ - +)|a232>g,I78>V(+ - +)|c268>g,Q90>E(+ + -)|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|g303>a|a308>t,Y103>F(- + -)| a81>t|c86>g,T29>S(+ - +)|t90>a,F30>L(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g119>a,S40>N(- - -)|g150>c|a164>t,N55>I(- - -)| c172>a,Q58>K(+ - -)|g192>a|a193>g,K65>E(+ - -)| t196>c,Y66>L(- - -)|a197>t,Y66>L(- - -)|g202>t,V68>L(+ - +)|a232>g,I78>V(+ - +)|c268>g,Q90>E(+ + -)|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|g303>a|a308>t,Y103>F(- + -)| a316>g,R106>G(- - -)|a318>g,R106>G(- - -)|a320>g| 315 (320) 220 (225) 200 (202) 20 (23) 4 (5) 16 (18) 3 (6) 4 0 1 1 1 4 1 2 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 1 1 0 0 0 0 1 1 0 1 0 0 51 51 48 3 1 2 0 1 0 0 0 0 1 0 1 0 0 0 30 24 21 3 1 2 1 1 0 0 0 1 0 0 0 0 0 0 117 114 105 9 1 8 1 1 0 1 1 0 2 0 1 1 1 0 3 (8) 3 3 (5) 0 (3) 0 (1) 0 (2) 0 (3) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggtttcagcttaagtgactattgg atgaactgggtccgccaggctccagggaaggggctcgagtgggtggccatc ataaagaaagatggaagtgaagaa ctctatttggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcactgtatctggaaatgaacagcctgagccccgaggacacggctgtatatttctgt gcggggggcagaggctggcttattgactac AGGRGWLIDY IGHV3-7 IGHD6-19 IGHJ4 ggtttcagcttaagtgactattgg atgaactgggtccgccaggctccagggaaggggctcgagtgggtggccatc ataaagaaagatggaagtgaagaa ctctatttggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcactgtatctggaaatgaacagcctgagccccgaggacacggctgtatatttctgt gcggggggcagaggctggcttattgactac IGG2 20 213 8 12 4 10 4 10 2 2 10 6 JY8QFUQ01A3VGZ 5214 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 905 90.83 198/218 nt 89.45 195/218 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [2.16.38.11] CVRAGFGELSGFDCW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'C' (1 nucleotides at position 17 to 17 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 47 of V-REGION: 'G' (1 nucleotides at position 56 to 56 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 40 of V-REGION: 2 nucleotides (from position 36 in the user submitted sequence), (cause frameshift) gcctcctggattcaccCtttggttattatggcatgactggtccgccaagctccggGggagggggctggagtgggtctctggtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [291] a72>g|g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>P(+ - -)|a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|t121>c,W41>L(+ - -)|g122>t,W41>L(+ - -)|a138>g|a143>g,K48>R(+ + +)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a72>g|g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>P(+ - -)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| t121>c,W41>L(+ - -)|g122>t,W41>L(+ - -)|a138>g|a143>g,K48>R(+ + +)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 225 (226) 204 21 (22) 4 (5) 17 4 3 2 3 1 (2) 0 2 0 3 1 2 0 78 7 3 4 1 3 1 0 1 1 0 0 0 0 1 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 49 45 4 1 3 2 0 0 1 0 0 0 0 0 0 1 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| gcctcct ggattcacctttggttattatggc atgactggtccgccaagctccggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgactggtccgccaagctccggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 16 211 8 8 3 8 5 8 3 0 9 4 JY8QFUQ01A3VOS 11209 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-3*01 F 1 8 8 10 8.8.10 [1.17.38.11] CARGGLEPVDYW in-frame + ctctggattcaccttcagtacatactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcccgtatcaatcctgatgggcgaatcacaaactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggactcggctgtgtactactgtgcaaggggtggtttggagcccgtagattattggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggcaactcag NA IGG1 100 100 [279] g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|a162>c|t168>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|g216>t,K72>N(+ - -)|a295>t,T99>S(+ - +)|t306>c| g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)| a162>c| t168>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|g216>t,K72>N(+ - -)|a295>t,T99>S(+ - +)|t306>c| a318>g|a320>g| 317 (320) 222 (225) 209 (210) 13 (15) 3 (5) 10 0 (2) 1 0 2 3 1 1 1 2 0 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 1 0 0 1 0 0 0 51 51 50 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 30 24 18 6 1 5 0 0 0 1 2 0 0 1 1 0 1 0 117 114 110 4 1 3 0 1 0 1 0 0 1 0 0 0 1 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtacatactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcccgt atcaatcctgatgggcgaatcaca aactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggactcggctgtgtactactgt gcaaggggtggtttggagcccgtagattat ARGGLEPVDY IGHV3-74 IGHD3-3 IGHJ4 ggattcaccttcagtacatactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcccgt atcaatcctgatgggcgaatcaca aactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggactcggctgtgtactactgt gcaaggggtggtttggagcccgtagattat IGG1 13 213 3 10 1 6 2 7 2 1 3 7 JY8QFUQ01A3W9F 18193 productive Homsap IGHV4-4*07 F 877 89.81 194/216 nt NA Homsap IGHJ4*02 F (see comment) 213 93.75 45/48 nt Homsap IGHD4-17*01 F 2 8 7 12 8.7.12 [2.17.38.11] CARFSSYGDTFDSW in-frame - Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) gtctctgatgggtccatcggaagttactactggacttggatccggcagcccgccgggaaggcaatggagtggatcgggcgtgtctttagaactgggaacacgaattacaacccctccctcaagagtcgggtcaccatgtcagttgacacgtccaagaatcaattctccctgaagctgagctctgtgaccgccgcggacacggccgtgttttactgtgcgagattctcgtcctacggtgacacctttgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 100 [283] g80>a,G27>D(- - -)|c84>g|a103>g,S35>G(+ + -)|t105>a,S35>G(+ + -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g146>c,G49>A(- + -)|c148>a,L50>M(+ + -)|t159>c|a166>g,I56>V(+ - +)|a170>t,Y57>F(- + -)|c173>g,T58>R(- - -)|c174>a,T58>R(- - -)|g176>c,S59>T(+ - +)|g191>a,S64>N(- - -)|c195>g|c198>t|a225>g|a240>t|c255>t|g258>a|a305>t,Y102>F(- + -)| g80>a,G27>D(- - -)|c84>g|a103>g,S35>G(+ + -)|t105>a,S35>G(+ + -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g146>c,G49>A(- + -)|c148>a,L50>M(+ + -)|t159>c| a166>g,I56>V(+ - +)|a170>t,Y57>F(- + -)|c173>g,T58>R(- - -)|c174>a,T58>R(- - -)|g176>c,S59>T(+ - +)|g191>a,S64>N(- - -)|c195>g| c198>t|a225>g|a240>t|c255>t|g258>a|a305>t,Y102>F(- + -)| g319>t|a320>t| 318 (320) 222 (224) 200 22 (24) 8 (10) 14 3 3 3 1 0 2 3 (4) 1 3 3 0 (1) 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 0 0 0 0 0 1 0 1 0 0 51 51 46 5 1 4 0 0 1 1 0 1 0 0 2 0 0 0 30 21 14 7 1 6 1 1 0 0 0 1 1 0 1 2 0 0 117 114 108 6 5 1 1 1 2 0 0 0 2 0 0 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct gatgggtccatcggaagttactac tggacttggatccggcagcccgccgggaaggcaatggagtggatcgggcgt gtctttagaactgggaacacg aattacaacccctccctcaagagtcgggtcaccatgtcagttgacacgtccaagaatcaattctccctgaagctgagctctgtgaccgccgcggacacggccgtgttttactgt gcgagattctcgtcctacggtgacacctttgactcc ARFSSYGDTFDS IGHV4-4 IGHD4-17 IGHJ4 gatgggtccatcggaagttactac tggacttggatccggcagcccgccgggaaggcaatggagtggatcgggcgt gtctttagaactgggaacacg aattacaacccctccctcaagagtcgggtcaccatgtcagttgacacgtccaagaatcaattctccctgaagctgagctctgtgaccgccgcggacacggccgtgttttactgt gcgagattctcgtcctacggtgacacctttgactcc IGA1 22 210 10 12 6 14 4 8 6 2 5 9 JY8QFUQ01A3YON 6335 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 1008 96.31 209/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD4-23*01 ORF 2 8 8 14 8.8.14 [1.17.38.11] CAKDRVYGGGEPLDHW in-frame + ctctggattcaccttcagtagttataacatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagttatatcatatgatggaagaaataaatactatgcagactccgtgaagggccgactcaccatctccagagacaattccaagaacatgttgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtattactgtgcgaaagatcgggtctacggagggggcgagccgcttgaccactggggccagggaaccctggtcagcgtctcctctgcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [291] c108>t|g112>a,G38>N(- - -)|g113>a,G38>N(- - -)|t189>a,S63>R(- - -)|t226>c,F76>L(+ - -)|c257>t,T86>M(- - -)|c259>t|g286>c,A96>P(- - -)|g317>a,R106>K(+ + +)| c108>t|g112>a,G38>N(- - -)|g113>a,G38>N(- - -)| t189>a,S63>R(- - -)| t226>c,F76>L(+ - -)|c257>t,T86>M(- - -)|c259>t|g286>c,A96>P(- - -)| g317>a,R106>K(+ + +)| 320 225 216 9 2 7 0 3 3 1 0 0 0 1 1 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 2 1 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 23 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 117 114 110 4 1 3 0 0 2 1 0 0 0 0 1 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagttataac atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcatatgatggaagaaataaa tactatgcagactccgtgaagggccgactcaccatctccagagacaattccaagaacatgttgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtattactgt gcgaaagatcgggtctacggagggggcgagccgcttgaccac AKDRVYGGGEPLDH IGHV3-30 IGHD4-23 IGHJ4 ggattcaccttcagtagttataac atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcatatgatggaagaaataaa tactatgcagactccgtgaagggccgactcaccatctccagagacaattccaagaacatgttgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtattactgt gcgaaagatcgggtctacggagggggcgagccgcttgaccac IGG1 8 213 6 2 5 6 1 2 1 1 3 3 JY8QFUQ01A3ZB9 16840 productive Homsap IGHV1-3*01 F 939 93.46 200/214 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD1-26*01 F 2 8 8 14 8.8.14 [0.17.38.11] CARDGESLGGDYFDYW in-frame + tggatacaccttcagtaattatgctatacattggttgcgccaggcccccggacaaaggcttgagtggatgggatggatcaacgctggcacgggtaacacaaaatattcacagaagttccagggcagagtcaccattaccagggacacagccgcgaccacagcctacatggaattgagcagcctgaaatctgaggacacggccgtgtattactgtgcgagagatggggagtcactgggaggcgactattttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [287] c104>g,T35>S(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g117>a,M39>I(+ + -)|g124>t,V42>L(+ - +)|a185>c,N62>T(- + -)|t186>g,N62>T(- + -)|t247>g,S83>A(- + -)|g254>c,S85>T(+ - +)|g270>a|c271>t|g284>a,R95>K(+ + +)|a291>g|t300>c| c104>g,T35>S(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g117>a,M39>I(+ + -)|g124>t,V42>L(+ - +)| a185>c,N62>T(- + -)|t186>g,N62>T(- + -)| t247>g,S83>A(- + -)|g254>c,S85>T(+ - +)|g270>a|c271>t|g284>a,R95>K(+ + +)|a291>g|t300>c| 320 222 208 14 4 10 1 4 2 1 1 0 0 0 1 1 1 2 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 1 0 0 0 0 0 0 1 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 0 0 1 0 30 24 22 2 0 2 0 0 0 0 1 0 0 0 0 0 0 1 117 114 107 7 4 3 1 2 1 1 0 0 0 0 1 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|ta,262-263(FR3)|aa,290-291(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,11-12(FR1)|tt,62-63(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,120-121(FR2)|tt,149-150(FR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,201-202(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcagtaattatgct atacattggttgcgccaggcccccggacaaaggcttgagtggatgggatgg atcaacgctggcacgggtaacaca aaatattcacagaagttccagggcagagtcaccattaccagggacacagccgcgaccacagcctacatggaattgagcagcctgaaatctgaggacacggccgtgtattactgt gcgagagatggggagtcactgggaggcgactattttgactac ARDGESLGGDYFDY IGHV1-3 IGHD1-26 IGHJ4 ggatacaccttcagtaattatgct atacattggttgcgccaggcccccggacaaaggcttgagtggatgggatgg atcaacgctggcacgggtaacaca aaatattcacagaagttccagggcagagtcaccattaccagggacacagccgcgaccacagcctacatggaattgagcagcctgaaatctgaggacacggccgtgtattactgt gcgagagatggggagtcactgggaggcgactattttgactac IGA1 14 213 8 6 6 9 2 5 4 0 5 5 JY8QFUQ01A3ZD4 16609 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*02 F or Homsap IGHV4-59*07 F or Homsap IGHV4-61*01 F or Homsap IGHV4-61*08 F 854 90.78 187/206 nt 90.29 186/206 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD1-1*01 F 3 5 7 12 5.7.12 [1.17.38.11] CARHPKSNVEGDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 30 of V-REGION: 9 nucleotides (from position 15 in the user submitted sequence), (do not cause frameshift) tctctggtgactctcacttctggagctggatccggcagcccccagggaagggcccggagtggattggttatgtctataacagtgggaccaccaactacaacccctccctcaggagtcgagtcaccatttctatcgacacgtccaagaagcagatctccctgaagttgaactctgtgaccgctgcggacacggccgtgtattactgtgcgagacacccgaagtccaacgtagagggtgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [273] g83>a,G28>D(- - -)|c87>t|t109>c,Y37>H(+ - -)|a113>t,Y38>F(- + -)|a147>c|t149>c,L50>P(- - -)|g162>t|a166>g,I56>V(+ - +)|t172>a,Y58>N(- - -)|g191>c,S64>T(+ - +)|a215>g,K72>R(+ + +)|a234>t|a237>t|g238>a,V80>I(+ - +)|a240>c,V80>I(+ - +)|c255>g,N85>K(+ - -)|t259>a,F87>I(+ - -)|c271>t|g275>a,S92>N(- - -)| g83>a,G28>D(- - -)|c87>t|t109>c,Y37>H(+ - -)|a113>t,Y38>F(- + -)| a147>c|t149>c,L50>P(- - -)|g162>t| a166>g,I56>V(+ - +)|t172>a,Y58>N(- - -)|g191>c,S64>T(+ - +)| a215>g,K72>R(+ + +)|a234>t|a237>t|g238>a,V80>I(+ - +)|a240>c,V80>I(+ - +)|c255>g,N85>K(+ - -)|t259>a,F87>I(+ - -)|c271>t|g275>a,S92>N(- - -)| g319>c| 318 (320) 212 (214) 193 (194) 19 (20) 6 (7) 13 2 3 2 2 2 0 3 2 1 (2) 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 15 11 4 1 3 0 1 1 1 0 0 1 0 0 0 0 0 51 51 48 3 2 1 0 0 0 1 1 0 0 0 0 0 1 0 30 21 18 3 0 3 1 0 0 0 0 0 0 1 1 0 0 0 117 114 105 9 3 6 1 2 1 0 1 0 2 1 0 1 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactctcacttc tggagctggatccggcagcccccagggaagggcccggagtggattggttat gtctataacagtgggaccacc aactacaacccctccctcaggagtcgagtcaccatttctatcgacacgtccaagaagcagatctccctgaagttgaactctgtgaccgctgcggacacggccgtgtattactgt gcgagacacccgaagtccaacgtagagggtgactac ARHPKSNVEGDY IGHV4-59 IGHD1-1 IGHJ4 ggtgactctcacttc tggagctggatccggcagcccccagggaagggcccggagtggattggttat gtctataacagtgggaccacc aactacaacccctccctcaggagtcgagtcaccatttctatcgacacgtccaagaagcagatctccctgaagttgaactctgtgaccgctgcggacacggccgtgtattactgt gcgagacacccgaagtccaacgtagagggtgactac IGA1 19 201 9 10 5 8 4 11 5 1 7 6 JY8QFUQ01A44PN 17377 productive Homsap IGHV4-31*02 F, or Homsap IGHV4-31*03 F or Homsap IGHV4-31*04 F 1073 98.64 218/221 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-6*01 F 1 10 7 16 10.7.16 [1.17.38.11] CARDRGPSSSSKMEYDYW in-frame + tctctggtggctccatcaacagtggtggttactactggagttggatccgccagcacccagggaagggcctggagtggattgggtacatctattacagtgggagtacctactacaacccgtccctcaagagtcgagttaccatatcagtagacacgtctaagaaccagttctccctgaagctgagctctgtgactgccgcggacacggccgtgtattactgtgcgagagatcggggccctagcagctcgtccaaaatggaatatgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 94 [300, 301] g92>a,S31>N(- - -)|c120>t|c192>t| g92>a,S31>N(- - -)| c120>t| c192>t| 320 229 226 3 2 1 0 1 2 0 0 0 0 0 0 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 29 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 21 20 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,228-229(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,69-70(FR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,227-228(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|agca,131-134(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agtt,225-228(FR3)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,69-72(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|tacc,228-231(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tgcc,285-288(FR3)|tact,307-310(FR3)| tctct ggtggctccatcaacagtggtggttactac tggagttggatccgccagcacccagggaagggcctggagtggattgggtac atctattacagtgggagtacc tactacaacccgtccctcaagagtcgagttaccatatcagtagacacgtctaagaaccagttctccctgaagctgagctctgtgactgccgcggacacggccgtgtattactgt gcgagagatcggggccctagcagctcgtccaaaatggaatatgactac ARDRGPSSSSKMEYDY IGHV4-31 IGHD6-6 IGHJ4 ggtggctccatcaacagtggtggttactac tggagttggatccgccagcacccagggaagggcctggagtggattgggtac atctattacagtgggagtacc tactacaacccgtccctcaagagtcgagttaccatatcagtagacacgtctaagaaccagttctccctgaagctgagctctgtgactgccgcggacacggccgtgtattactgt gcgagagatcggggccctagcagctcgtccaaaatggaatatgactac IGG1 3 216 3 0 3 3 0 0 1 1 0 1 JY8QFUQ01A453G 5215 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 879 89.09 196/220 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD6-13*01 F 2 8 8 12 8.8.12 [2.17.38.11] CVREGAATGSFYYW in-frame - aacctctggattcaccttcagtgactatgccatgagctgggtccgccaggctccagcgaaggggctggaatgggtctcagcgattagcagtagtggtgatagaacatactacgcagactccgtgaagggccgattcaccatctccagagacagttccagggggactctgtatttgcaaatgaaccgcctgagcgccgaggacacggccctatatttctgtgtgagagaaggagcagcaactggttcgttttactactggggccagggagccctggtcaccgtctcctcatcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] g73>a,A25>T(- - -)|t90>c|c105>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g140>c,G47>A(- + -)|g153>a|t165>g|t171>c|g172>a,G58>S(+ + -)|g188>a,G63>D(- - -)|c192>a,S64>R(- - -)|g225>a|a245>g,N82>S(- - -)|a251>g,K84>R(+ + +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|g258>t|c265>t|a277>c,S93>R(- - -)|a285>c,R95>S(- - -)|g301>c,V101>L(+ - +)|a308>t,Y103>F(- + -)|c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)| g73>a,A25>T(- - -)| t90>c|c105>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g140>c,G47>A(- + -)|g153>a|t165>g| t171>c|g172>a,G58>S(+ + -)|g188>a,G63>D(- - -)|c192>a,S64>R(- - -)| g225>a|a245>g,N82>S(- - -)|a251>g,K84>R(+ + +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|g258>t|c265>t|a277>c,S93>R(- - -)|a285>c,R95>S(- - -)|g301>c,V101>L(+ - +)|a308>t,Y103>F(- + -)| c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)| 320 228 202 26 8 18 6 6 3 2 2 1 1 0 2 1 1 1 78 7 6 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 1 1 1 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 0 0 0 0 0 0 1 0 0 1 30 24 20 4 1 3 0 2 0 1 0 1 0 0 0 0 0 0 117 114 102 12 3 9 4 1 1 0 2 0 1 0 1 1 1 0 8 8 6 2 0 2 1 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| aacctct ggattcaccttcagtgactatgcc atgagctgggtccgccaggctccagcgaaggggctggaatgggtctcagcg attagcagtagtggtgatagaaca tactacgcagactccgtgaagggccgattcaccatctccagagacagttccagggggactctgtatttgcaaatgaaccgcctgagcgccgaggacacggccctatatttctgt gtgagagaaggagcagcaactggttcgttttactac VREGAATGSFYY IGHV3-23 IGHD6-13 IGHJ4 ggattcaccttcagtgactatgcc atgagctgggtccgccaggctccagcgaaggggctggaatgggtctcagcg attagcagtagtggtgatagaaca tactacgcagactccgtgaagggccgattcaccatctccagagacagttccagggggactctgtatttgcaaatgaaccgcctgagcgccgaggacacggccctatatttctgt gtgagagaaggagcagcaactggttcgttttactac IGA1 23 213 14 9 7 12 7 11 5 3 10 5 JY8QFUQ01A45ND 10585 productive Homsap IGHV1-46*01 F, or Homsap IGHV1-46*03 F 1002 96.73 207/214 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-13*01 F 3 8 8 11 8.8.11 [0.17.38.11] CARGQQLRDFYYW in-frame - tggatacaccttcaccagctactatatacactgggtgcgacaggcccctggacaagggcttgagtggatgggaataatcgaccctagtggtggtgccacaagctacgcacagcagttccagggcagagtcaccatgaccagggacacgtccacgagcacagtctatatggagctgagcagcctgagatctgacgacacggccgtgtattactgtgcgaggggacagcagcttcgagacttttactactggggccagggaaccctggtcaccgtcccctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [278] g117>a,M39>I(+ + -)|a169>g,N57>D(+ + -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|a208>c,K70>Q(+ - -)|c264>t|g291>c,E97>D(+ - +)| g117>a,M39>I(+ + -)| a169>g,N57>D(+ + -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| a208>c,K70>Q(+ - -)|c264>t|g291>c,E97>D(+ - +)| a318>g|a320>g| 317 (320) 219 (222) 212 (213) 7 (9) 1 (3) 6 2 (4) 1 1 0 1 0 0 0 2 0 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 2 0 0 0 0 0 0 0 1 0 0 0 117 114 111 3 1 2 0 0 1 0 1 0 0 0 1 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,162-163(FR2)|ta,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,62-63(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,164-165(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggca,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|aacc,169-172(CDR2)|agct,196-199(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcaccagctactat atacactgggtgcgacaggcccctggacaagggcttgagtggatgggaata atcgaccctagtggtggtgccaca agctacgcacagcagttccagggcagagtcaccatgaccagggacacgtccacgagcacagtctatatggagctgagcagcctgagatctgacgacacggccgtgtattactgt gcgaggggacagcagcttcgagacttttactac ARGQQLRDFYY IGHV1-46 IGHD6-13 IGHJ4 ggatacaccttcaccagctactat atacactgggtgcgacaggcccctggacaagggcttgagtggatgggaata atcgaccctagtggtggtgccaca agctacgcacagcagttccagggcagagtcaccatgaccagggacacgtccacgagcacagtctatatggagctgagcagcctgagatctgacgacacggccgtgtattactgt gcgaggggacagcagcttcgagacttttactac IGA1 7 213 4 3 2 4 2 3 1 0 3 3 JY8QFUQ01A465M 15073 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 788 84.86 185/218 nt 83.94 183/218 nt Homsap IGHJ3*01 F 169 82 41/50 nt Homsap IGHD3-16*01 F 3 8 8 12 8.8.12 [1.17.38.11] CARDDGGHMTLDVW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (84,86% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR2-IMGT at codon 65 of V-REGION: 'C' (1 nucleotides at position 102 to 102 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 84 of V-REGION: 'G' (1 nucleotides at position 159 to 159 in the user submitted sequence), (cause frameshift); gtctgggattcacatttagaagctattccatgaattgggtccgccaggctccagggaaggggctggagtgggtctcagctgtcagtggtggtggggggcgcCacaaactacgcggagtccgtgaagggccggttcaccatctccagagacaattccagGggggacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgtgcgagagatgacggcggacacatgactttagatgtctggggccaagggacaatggtcagcgtctctccggcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [288] c74>g|c75>t|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)|c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a175>g,S59>G(+ + -)|t186>g|t189>g|a190>c,S64>R(- - -)|t196>a,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|a251>g,K84>R(+ + +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|c287>t,A96>V(+ - +)|g291>a|g297>a|g301>t,V101>L(+ - +)|c309>t|a317>g,K106>R(+ + +)| c74>g|c75>t|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)| c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a175>g,S59>G(+ + -)|t186>g|t189>g|a190>c,S64>R(- - -)| t196>a,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|a251>g,K84>R(+ + +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|c287>t,A96>V(+ - +)|g291>a|g297>a|g301>t,V101>L(+ - +)|c309>t| a317>g,K106>R(+ + +)| 320 226 192 34 11 23 7 4 6 3 1 2 1 1 0 4 2 3 78 5 0 5 2 3 0 0 2 1 0 0 0 0 0 1 0 1 36 24 21 3 1 2 0 0 0 0 0 2 0 0 0 0 1 0 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 24 18 6 2 4 2 0 0 1 1 0 0 0 0 0 0 2 117 114 97 17 6 11 4 3 3 1 0 0 1 1 0 3 1 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtctg ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagct gtcagtggtggtggggggcgcaca aactacgcggagtccgtgaagggccggttcaccatctccagagacaattccagggggacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgt gcgagagatgacggcggacacatgactttagatgtc ARDDGGHMTLDV IGHV3-23 IGHD3-16 IGHJ3 ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagct gtcagtggtggtggggggcgcaca aactacgcggagtccgtgaagggccggttcaccatctccagagacaattccagggggacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgt gcgagagatgacggcggacacatgactttagatgtc IGG1 28 213 16 12 8 15 8 13 6 3 13 6 JY8QFUQ01A476X 7903 productive Homsap IGHV3-11*01 F 945 93.09 202/217 nt NA Homsap IGHJ6*02 F (see comment) 175 75.81 47/62 nt Homsap IGHD2-21*01 F 3 8 8 12 8.8.12 [1.17.38.11] CARGKMVSTGMDVW in-frame + Other possibilities: Homsap_IGHJ5*01 (shorter alignment but highest percentage of identity) ctctggattcacgtttagtgactactacatgacctggattcgtcaggctccagggaagggcctggagtgggttacatatattagtagtagtggtggtaacacacattacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaacgcactgtatctgcaaatgaacagcctgagggccgaggacacggccgtgtattactgtgcgcgagggaagatggtgagcaccggtatggacgtctggggccaagggaccacggtcgccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [284] c87>g|c90>t|g119>c,S40>T(+ - +)|c126>t|c129>t|g147>c|t160>a,S54>T(+ - +)|c165>t|a187>g,S63>G(+ + -)|c191>a,T64>N(- + -)|t194>c,I65>T(- - -)|t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|t256>g,S86>A(- + -)|a285>g|a316>c| c87>g|c90>t| g119>c,S40>T(+ - +)|c126>t|c129>t|g147>c|t160>a,S54>T(+ - +)|c165>t| a187>g,S63>G(+ + -)|c191>a,T64>N(- + -)|t194>c,I65>T(- - -)| t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|t256>g,S86>A(- + -)|a285>g| a316>c|a320>g| 319 (320) 224 (225) 208 16 (17) 8 (9) 8 2 (3) 0 5 2 1 1 0 1 2 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 2 0 0 0 1 0 0 0 0 0 0 1 0 0 51 51 45 6 4 2 0 0 3 0 0 0 0 1 2 0 0 0 30 24 21 3 0 3 1 0 0 1 0 1 0 0 0 0 0 0 117 114 110 4 1 3 1 0 1 1 0 0 0 0 0 0 0 1 7 (8) 7 6 1 (2) 1 (2) 0 0 (1) 0 0 0 1 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgtttagtgactactac atgacctggattcgtcaggctccagggaagggcctggagtgggttacatat attagtagtagtggtggtaacaca cattacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaacgcactgtatctgcaaatgaacagcctgagggccgaggacacggccgtgtattactgt gcgcgagggaagatggtgagcaccggtatggacgtc ARGKMVSTGMDV IGHV3-11 IGHD2-21 IGHJ6 ggattcacgtttagtgactactac atgacctggattcgtcaggctccagggaagggcctggagtgggttacatat attagtagtagtggtggtaacaca cattacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaacgcactgtatctgcaaatgaacagcctgagggccgaggacacggccgtgtattactgt gcgcgagggaagatggtgagcaccggtatggacgtc IGA1 15 213 9 6 5 9 4 6 5 2 5 3 JY8QFUQ01A47U3 7342 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 1045 97.72 214/219 nt NA Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD2-21*02 F 2 8 8 17 8.8.17 [2.17.38.11] CARPYCGGDCHNRGAFDIW in-frame - gcgtctggattcaccttcagtagctatggcatacactgggtccgccaggctccaggcaaggggctggagtgggtggcagttatatggtatgatggaagtgaaatatactatgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgaggccatattgtggtggtgactgccacaatcgaggggcttttgatatctggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [301, 300] g117>a,M39>I(+ + -)|a190>g,N64>E(+ - -)|t192>a,N64>E(+ - -)|a194>t,K65>I(- + -)|a225>c| g117>a,M39>I(+ + -)| a190>g,N64>E(+ - -)|t192>a,N64>E(+ - -)|a194>t,K65>I(- + -)| a225>c| a318>g|g319>c|a320>c| 317 (320) 224 (227) 219 5 (8) 1 (4) 4 1 (2) 1 0 0 1 (2) 0 1 1 0 (1) 0 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 1 1 0 0 0 0 117 114 113 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcgtct ggattcaccttcagtagctatggc atacactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatggtatgatggaagtgaaata tactatgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgaggccatattgtggtggtgactgccacaatcgaggggcttttgatatc ARPYCGGDCHNRGAFDI IGHV3-33 IGHD2-21 IGHJ3 ggattcaccttcagtagctatggc atacactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatggtatgatggaagtgaaata tactatgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgaggccatattgtggtggtgactgccacaatcgaggggcttttgatatc IGA2 5 213 2 3 1 1 1 4 1 0 1 3 JY8QFUQ01A481H 7512 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 964 93.61 205/219 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-2*01 F 3 8 8 11 8.8.11 [2.17.38.11] CARFGPLTRPDYW in-frame - gcctctggattcacctttaccaactatggcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtggtgatagaacataccacgcagactccgtgcagggccggttcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagccgacgacgcgggcgtatattactgtgcgagattcggaccacttactcgacctgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [284] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)|c164>g,A55>G(- + -)|g188>a,G63>D(- - -)|c192>a,S64>R(- - -)|t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|t246>c|g258>t|c279>t|g291>c,E97>D(+ - +)|a295>g,T99>A(- - -)|c299>g,A100>G(- + -)| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)| c164>g,A55>G(- + -)| g188>a,G63>D(- - -)|c192>a,S64>R(- - -)| t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|t246>c|g258>t|c279>t|g291>c,E97>D(+ - +)|a295>g,T99>A(- - -)|c299>g,A100>G(- + -)| a317>g,K106>R(+ + +)|g319>t|a320>t| 316 (320) 223 (227) 209 (210) 14 (17) 3 (5) 11 (12) 1 (2) 2 1 2 1 1 0 (1) 0 2 3 1 (2) 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 22 2 0 2 0 1 0 0 0 1 0 0 0 0 0 0 117 114 106 8 3 5 1 0 1 2 1 0 0 0 1 1 1 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgatagaaca taccacgcagactccgtgcagggccggttcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagccgacgacgcgggcgtatattactgt gcgagattcggaccacttactcgacctgactac ARFGPLTRPDY IGHV3-23 IGHD2-2 IGHJ4 ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgatagaaca taccacgcagactccgtgcagggccggttcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagccgacgacgcgggcgtatattactgt gcgagattcggaccacttactcgacctgactac IGG2 14 213 6 8 3 10 3 4 3 0 6 5 JY8QFUQ01A48DI 3777 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 936 92.63 201/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-10*01 F 2 8 8 13 8.8.13 [1.17.38.11] CANWLSGTHSKFDYW in-frame - ctctggattcaccttcagcaagtatgccatgagctgggtccgccaggctccaggggaggggctgcagtgggtctcagcaattagtggaaatggtgctgatatatactacgcagactccgtgaacggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgtgcgaattggctttcggggacacattctaagtttgactactggggcaagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [287, 286] t90>c|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a142>g,K48>E(+ - -)|g151>c,E51>Q(+ + -)|t165>a|t174>a|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c192>t,S64>D(- - -)|c194>t,T65>I(- - -)|g216>c,K72>N(+ - -)|g261>a|t306>c| t90>c|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)| a142>g,K48>E(+ - -)|g151>c,E51>Q(+ + -)|t165>a| t174>a|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c192>t,S64>D(- - -)|c194>t,T65>I(- - -)| g216>c,K72>N(+ - -)|g261>a|t306>c| a318>t,K106>N(+ - -)|g319>t|a320>g| 317 (320) 222 (225) 206 16 (19) 5 (7) 11 (12) 2 (3) 4 2 2 0 0 0 (1) 2 3 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 0 1 0 0 0 0 0 1 0 0 51 51 48 3 1 2 1 0 0 0 0 0 0 1 1 0 0 0 30 24 17 7 1 6 1 2 2 0 0 0 0 1 1 0 0 0 117 114 111 3 2 1 0 1 0 1 0 0 0 0 1 0 0 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagcaagtatgcc atgagctgggtccgccaggctccaggggaggggctgcagtgggtctcagca attagtggaaatggtgctgatata tactacgcagactccgtgaacggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgt gcgaattggctttcggggacacattctaagtttgactac ANWLSGTHSKFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcaccttcagcaagtatgcc atgagctgggtccgccaggctccaggggaggggctgcagtgggtctcagca attagtggaaatggtgctgatata tactacgcagactccgtgaacggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgt gcgaattggctttcggggacacattctaagtttgactac IGA2 16 213 10 6 6 10 4 6 3 2 3 8 JY8QFUQ01A4AB0 13116 productive Homsap IGHV3-30*01 F, or Homsap IGHV3-30*11 F 967 94.44 204/216 nt NA Homsap IGHJ6*02 F (see comment) 202 80.65 50/62 nt Homsap IGHD2-2*01 F 2 8 8 14 8.8.14 [1.17.38.11] CVREGRGTAAYGLDVW in-frame - Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) tctggattcaccttcagtagctatgctatgcactgggtccgccagactccaggcaagggactagagtgggtggcagttatatcatatgatggaagtgactacgactacgcaggctccgtgaagggccgattcaccatctccagagacagttccaagaacatgctgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtatcactgtgtgagagagggtcgtggtaccgccgcctacggtctggacgtctggggccgagggaccacggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG4 100 88 [290] g133>a,A45>T(- - -)|g147>a|a190>g,N64>D(+ + -)|t192>c,N64>D(+ + -)|a193>t,K65>Y(- - -)|a195>c,K65>Y(- - -)|t196>g,Y66>D(- - -)|a206>g,D69>G(- - -)|a245>g,N82>S(- - -)|c257>t,T86>M(- - -)|g286>c,A96>P(- - -)|t307>c,Y103>H(+ - -)|c314>t,A105>V(+ - +)| g133>a,A45>T(- - -)|g147>a| a190>g,N64>D(+ + -)|t192>c,N64>D(+ + -)|a193>t,K65>Y(- - -)|a195>c,K65>Y(- - -)| t196>g,Y66>D(- - -)|a206>g,D69>G(- - -)|a245>g,N82>S(- - -)|c257>t,T86>M(- - -)|g286>c,A96>P(- - -)|t307>c,Y103>H(+ - -)| c314>t,A105>V(+ - +)| 320 224 211 13 1 12 3 2 2 2 1 0 1 0 1 0 0 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 0 1 1 0 1 0 0 0 0 0 117 114 108 6 0 6 2 0 1 1 0 0 0 0 1 0 0 1 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcagtagctatgct atgcactgggtccgccagactccaggcaagggactagagtgggtggcagtt atatcatatgatggaagtgactac gactacgcaggctccgtgaagggccgattcaccatctccagagacagttccaagaacatgctgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtatcactgt gtgagagagggtcgtggtaccgccgcctacggtctggacgtc VREGRGTAAYGLDV IGHV3-30 IGHD2-2 IGHJ6 ggattcaccttcagtagctatgct atgcactgggtccgccagactccaggcaagggactagagtgggtggcagtt atatcatatgatggaagtgactac gactacgcaggctccgtgaagggccgattcaccatctccagagacagttccaagaacatgctgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtatcactgt gtgagagagggtcgtggtaccgccgcctacggtctggacgtc IGG4 12 213 8 4 3 4 5 8 1 0 7 4 JY8QFUQ01A4BI4 2529 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 954 93.55 203/217 nt NA Homsap IGHJ6*02 F (see comment) 184 77.42 48/62 nt Homsap IGHD4-17*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKEMIRYGDDVFGGLNVW in-frame + Other possibilities: Homsap_IGHJ4*03 (shorter alignment but highest percentage of identity) ctctggatttacctttagcagttatgccatgaactgggtccgccaggctccagggaaggggctgcagtgggtctctggtattagtggtggtggtgatgacacatactacgcagactccgtgaagggccggttcaccgtctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtgtattactgtgcgaaagaaatgatccgctacggggacgacgtttttggcggtctgaacgtctggggccaagggaccgcggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [299] c84>t|c108>t|g119>a,S40>N(- - -)|g151>c,E51>Q(+ + -)|a162>t|c164>g,A55>G(- + -)|a175>g,S59>G(+ + -)|g188>a,G63>D(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a232>g,I78>V(+ - +)|a275>g,N92>S(- - -)|g291>a|a303>g| c84>t|c108>t| g119>a,S40>N(- - -)|g151>c,E51>Q(+ + -)|a162>t|c164>g,A55>G(- + -)| a175>g,S59>G(+ + -)|g188>a,G63>D(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a232>g,I78>V(+ - +)|a275>g,N92>S(- - -)|g291>a|a303>g| 320 225 211 14 5 9 5 4 2 0 0 0 1 0 1 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 2 0 0 0 2 0 0 0 0 0 0 0 0 0 51 51 47 4 1 3 0 1 0 0 0 0 1 0 1 1 0 0 30 24 20 4 0 4 2 2 0 0 0 0 0 0 0 0 0 0 117 114 110 4 2 2 3 1 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatttacctttagcagttatgcc atgaactgggtccgccaggctccagggaaggggctgcagtgggtctctggt attagtggtggtggtgatgacaca tactacgcagactccgtgaagggccggttcaccgtctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtgtattactgt gcgaaagaaatgatccgctacggggacgacgtttttggcggtctgaacgtc AKEMIRYGDDVFGGLNV IGHV3-23 IGHD4-17 IGHJ6 ggatttacctttagcagttatgcc atgaactgggtccgccaggctccagggaaggggctgcagtgggtctctggt attagtggtggtggtgatgacaca tactacgcagactccgtgaagggccggttcaccgtctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtgtattactgt gcgaaagaaatgatccgctacggggacgacgtttttggcggtctgaacgtc IGA1 14 213 11 3 6 8 5 6 3 2 5 4 JY8QFUQ01A4C55 10343 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*02 F or Homsap IGHV4-59*07 F 818 86.98 187/215 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-22*01 F 2 8 7 22 8.7.22 [1.17.38.11] CARVKTDYYDSSGSYLPLYFFDYW in-frame + tctctggtgactccttcagaactcactactggagttggattcgacagcccccggggaggagattggaatggattggcaatatatattatattgggaccaccaactacaacccctccctcaagagtcgagtcaccatgttagtggacacgtccaagaagcagttctccctgagactgagttctgtgaccgctgcggacacggccatgtattattgtgcgagggtcaagactgattattatgatagtagtggttcttacctgcccctgtatttctttgattactggggccagggaaccccggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgagctgcctggtcaaggactacttcccccgaaccggtgacggtgtcggtggaactca NA IGG1 100 100 [313] g83>a,G28>D(- - -)|a88>t,I30>F(+ - -)|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|t109>c,Y37>H(+ - -)|c120>t|c126>t|g129>a|a138>g|a143>g,K48>R(+ + +)|g145>a,G49>R(- - -)|c148>t|g153>a|g162>c|t163>a,Y55>N(- - -)|c168>a|c174>t|g176>t,S59>I(- - -)|g191>c,S64>T(+ - +)|a234>g,I78>M(+ + -)|c236>t,S79>L(- - -)|a240>g|c255>g,N85>K(+ - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c276>t|g301>a,V101>M(+ - -)|c309>t| g83>a,G28>D(- - -)|a88>t,I30>F(+ - -)|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|t109>c,Y37>H(+ - -)| c120>t|c126>t|g129>a|a138>g|a143>g,K48>R(+ + +)|g145>a,G49>R(- - -)|c148>t|g153>a|g162>c|t163>a,Y55>N(- - -)| c168>a|c174>t|g176>t,S59>I(- - -)|g191>c,S64>T(+ - +)| a234>g,I78>M(+ + -)|c236>t,S79>L(- - -)|a240>g|c255>g,N85>K(+ - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c276>t|g301>a,V101>M(+ - -)|c309>t| a318>g|a320>t| 317 (320) 220 (223) 192 (193) 28 (30) 12 (14) 16 5 (6) 6 7 1 0 1 1 (2) 2 3 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 1 0 1 0 0 1 1 1 0 0 0 51 51 41 10 7 3 2 3 3 0 0 0 0 1 1 0 0 0 30 21 17 4 2 2 0 0 1 0 0 1 0 0 1 0 1 0 117 114 105 9 3 6 3 2 3 0 0 0 0 0 0 1 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccttcagaactcactac tggagttggattcgacagcccccggggaggagattggaatggattggcaat atatattatattgggaccacc aactacaacccctccctcaagagtcgagtcaccatgttagtggacacgtccaagaagcagttctccctgagactgagttctgtgaccgctgcggacacggccatgtattattgt gcgagggtcaagactgattattatgatagtagtggttcttacctgcccctgtatttctttgattac ARVKTDYYDSSGSYLPLYFFDY IGHV4-59 IGHD3-22 IGHJ4 ggtgactccttcagaactcactac tggagttggattcgacagcccccggggaggagattggaatggattggcaat atatattatattgggaccacc aactacaacccctccctcaagagtcgagtcaccatgttagtggacacgtccaagaagcagttctccctgagactgagttctgtgaccgctgcggacacggccatgtattattgt gcgagggtcaagactgattattatgatagtagtggttcttacctgcccctgtatttctttgattac IGG1 28 210 19 9 13 19 6 9 10 2 9 7 JY8QFUQ01A4D0Z 5127 productive (see comment) Homsap IGHV1-3*01 F 966 94.86 203/214 nt 94.39 202/214 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-8*01 F 2 8 8 9 8.8.9 [0.17.38.11] CARWNNDLDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'G' (1 nucleotides at position 45 to 45 in the user submitted sequence), (cause frameshift); tggatacaccttcagtacctatactatgaattgggtgcgccaggGcccccggacaaaggcttgagtggatgggatggatcaacgctgaccttggcaacacaaaatattcacagaagttccagggcagactcaccattaccagggacacatccgcgaacacagcctacatggagctgagcagcctgacatctgaagacacggctgtgtattactgtgcgagatggaacaatgaccttgactattggggccagggaaccctagtcactgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [273, 272] c104>g,T35>S(+ - +)|g107>c,S36>T(+ - +)|g112>a,A38>T(- - -)|c118>a,H40>N(- - -)|g176>a,G59>D(- - -)|a184>c,N62>L(- - -)|a185>t,N62>L(- - -)|t189>c|g226>c,V76>L(+ - +)|g254>a,S85>N(- - -)|g284>c,R95>T(- - -)| c104>g,T35>S(+ - +)|g107>c,S36>T(+ - +)|g112>a,A38>T(- - -)| c118>a,H40>N(- - -)| g176>a,G59>D(- - -)|a184>c,N62>L(- - -)|a185>t,N62>L(- - -)|t189>c| g226>c,V76>L(+ - +)|g254>a,S85>N(- - -)|g284>c,R95>T(- - -)| g319>t|a320>g| 318 (320) 220 (222) 209 11 (13) 1 (3) 10 0 (1) 3 0 1 1 1 1 0 3 1 0 (1) 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 30 24 20 4 1 3 0 1 0 1 1 0 1 0 0 0 0 0 117 114 111 3 0 3 0 1 0 0 0 0 0 0 2 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|ta,262-263(FR3)|aa,290-291(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,11-12(FR1)|tt,62-63(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,120-121(FR2)|tt,149-150(FR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,201-202(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcagtacctatact atgaattgggtgcgccaggcccccggacaaaggcttgagtggatgggatgg atcaacgctgaccttggcaacaca aaatattcacagaagttccagggcagactcaccattaccagggacacatccgcgaacacagcctacatggagctgagcagcctgacatctgaagacacggctgtgtattactgt gcgagatggaacaatgaccttgactat ARWNNDLDY IGHV1-3 IGHD2-8 IGHJ4 ggatacaccttcagtacctatact atgaattgggtgcgccaggcccccggacaaaggcttgagtggatgggatgg atcaacgctgaccttggcaacaca aaatattcacagaagttccagggcagactcaccattaccagggacacatccgcgaacacagcctacatggagctgagcagcctgacatctgaagacacggctgtgtattactgt gcgagatggaacaatgaccttgactat IGA2 11 213 4 7 3 8 1 3 0 1 4 6 JY8QFUQ01A4E73 21828 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 906 90.45 199/220 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD5-12*01 F 3 8 8 10 8.8.10 [2.17.38.11] CARDLYGADDLW in-frame + aacctctggattcacctttagtagatattggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtattaatgaagatgggaggaccacaacctacgcggactccgtgaatggccgattcctcatctccagagacaacgccaagaatacgttgtatctgcagatgagcagtctgagagtcgaggacacggccatgtattattgtgcaagagatctatatggtgccgatgacctctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcgtcgcatgcctggtcccagggcttcttcccccc NA IGA2 93 100 [281, 282] g73>a,A25>T(- - -)|c90>t|c108>a,S36>R(- - -)|c111>t|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t189>g,S63>R(- - -)|g191>c,S64>T(+ - +)|g197>c,S66>T(+ - +)|g216>t,K72>N(+ - -)|a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|c255>t|c259>t|a270>g|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|t300>c|g301>a,V101>M(+ - -)|c309>t| g73>a,A25>T(- - -)| c90>t|c108>a,S36>R(- - -)|c111>t| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t189>g,S63>R(- - -)|g191>c,S64>T(+ - +)| g197>c,S66>T(+ - +)|g216>t,K72>N(+ - -)|a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|c255>t|c259>t|a270>g|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|t300>c|g301>a,V101>M(+ - -)|c309>t| 320 228 207 21 7 14 3 3 7 1 1 1 0 1 2 0 1 1 78 7 6 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 2 0 0 1 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 19 5 0 5 1 1 0 0 0 0 0 1 1 0 0 1 117 114 102 12 5 7 2 1 5 1 1 0 0 0 1 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| aacctct ggattcacctttagtagatattgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attaatgaagatgggaggaccaca acctacgcggactccgtgaatggccgattcctcatctccagagacaacgccaagaatacgttgtatctgcagatgagcagtctgagagtcgaggacacggccatgtattattgt gcaagagatctatatggtgccgatgacctc ARDLYGADDL IGHV3-74 IGHD5-12 IGHJ4 ggattcacctttagtagatattgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attaatgaagatgggaggaccaca acctacgcggactccgtgaatggccgattcctcatctccagagacaacgccaagaatacgttgtatctgcagatgagcagtctgagagtcgaggacacggccatgtattattgt gcaagagatctatatggtgccgatgacctc IGA2 20 213 13 7 9 13 4 7 5 2 7 6 JY8QFUQ01A4ENF 21325 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 860 88.53 193/218 nt 88.07 192/218 nt Homsap IGHJ3*01 F (see comment) 169 82 41/50 nt Homsap IGHD2-15*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARGAGAEGKGGMDVW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT at codon 35 of V-REGION: 'A' (1 nucleotides at position 19 to 19 in the user submitted sequence), (cause frameshift); cctctggattcaccttcaAaaaagtatggcatgaactggctccgccaggctccagggaaggggctggagtgggtcgcaaccattcgcagtagtggtacttccatacactatgccgactccgtgaagggccgattcactatcaccagagacaacgccaacaactcactgtatctgcaattgaacagcctgggagtcgaggactcggctgtgtatttctgtgcgagaggggccggcgcagaaggtaaaggtggtatggacgtctggggccaagggactacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [292] g104>a,S35>K(- - -)|t105>a,S35>K(- - -)|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a112>g,S38>G(+ + -)|g124>c,V42>L(+ - +)|t160>g,S54>A(- + -)|t163>a,S55>T(+ - +)|a169>c,S57>R(- - -)|t171>c,S57>R(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|a191>c,Y64>S(+ - -)|t196>c,Y66>H(+ - -)|c201>t|a204>c|a210>c|c231>t|t235>a,S79>T(+ - +)|g252>c,K84>N(+ - -)|a271>t,M91>L(+ + -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|a295>t,T99>S(+ - +)|a308>t,Y103>F(- + -)| g104>a,S35>K(- - -)|t105>a,S35>K(- - -)|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a112>g,S38>G(+ + -)| g124>c,V42>L(+ - +)|t160>g,S54>A(- + -)|t163>a,S55>T(+ - +)| a169>c,S57>R(- - -)|t171>c,S57>R(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|a191>c,Y64>S(+ - -)| t196>c,Y66>H(+ - -)|c201>t|a204>c|a210>c|c231>t|t235>a,S79>T(+ - +)|g252>c,K84>N(+ - -)|a271>t,M91>L(+ + -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|a295>t,T99>S(+ - +)|a308>t,Y103>F(- + -)| a320>g| 319 (320) 225 (226) 200 25 (26) 4 (5) 21 3 (4) 2 3 2 4 0 3 3 3 1 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 2 0 0 0 0 0 1 0 1 0 0 51 51 48 3 0 3 0 0 0 0 0 0 0 1 1 0 0 1 30 24 19 5 0 5 1 0 0 1 2 0 0 0 1 0 0 0 117 114 102 12 4 8 1 0 3 1 2 0 3 1 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcaaaaagtatggc atgaactggctccgccaggctccagggaaggggctggagtgggtcgcaacc attcgcagtagtggtacttccata cactatgccgactccgtgaagggccgattcactatcaccagagacaacgccaacaactcactgtatctgcaattgaacagcctgggagtcgaggactcggctgtgtatttctgt gcgagaggggccggcgcagaaggtaaaggtggtatggacgtc ARGAGAEGKGGMDV IGHV3-21 IGHD2-15 IGHJ3 ggattcaccttcaaaaagtatggc atgaactggctccgccaggctccagggaaggggctggagtgggtcgcaacc attcgcagtagtggtacttccata cactatgccgactccgtgaagggccgattcactatcaccagagacaacgccaacaactcactgtatctgcaattgaacagcctgggagtcgaggactcggctgtgtatttctgt gcgagaggggccggcgcagaaggtaaaggtggtatggacgtc IGA2 25 213 10 15 5 9 5 16 4 0 11 10 JY8QFUQ01A4HOH 13113 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 1080 100 217/217 nt NA Homsap IGHJ6*02 F 265 91.94 57/62 nt Homsap IGHD2-2*01 F 2 8 8 22 8.8.22 [1.17.38.11] CARGHCISTSCYWEIHYYYGMDVW in-frame + ctctggattcaccttcagtagctatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattagtagtagtagtagttacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagggcattgtattagtaccagctgctattgggagattcactactactacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [315] a320>g| 319 (320) 224 (225) 224 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtagtagttacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggcattgtattagtaccagctgctattgggagattcactactactacggtatggacgtc ARGHCISTSCYWEIHYYYGMDV IGHV3-21 IGHD2-2 IGHJ6 ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtagtagttacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggcattgtattagtaccagctgctattgggagattcactactactacggtatggacgtc IGG1 0 213 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01A4HT0 16233 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 944 93.49 201/215 nt 93.02 200/215 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 7 13 8.7.13 [1.17.38.11] CVRAGFGELSGFDCW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 65 of V-REGION: 2 nucleotides (from position 102 in the user submitted sequence), (cause frameshift) ctctggattcacctttggttattatggcatgagctgggtccgccaagctccggggagggggctggagtgggtctctggtattaatcggaatggtgatagcaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [286] a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a138>g|a143>g,K48>R(+ + +)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a138>g|a143>g,K48>R(+ + +)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 222 (223) 207 15 (16) 3 (4) 12 3 3 1 1 1 (2) 0 2 0 2 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 51 49 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 30 22 20 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ctct ggattcacctttggttattatggc atgagctgggtccgccaagctccggggagggggctggagtgggtctctggt attaatcggaatggtgatagca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgagctgggtccgccaagctccggggagggggctggagtgggtctctggt attaatcggaatggtgatagca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 14 211 7 7 3 7 4 7 3 0 7 4 JY8QFUQ01A4I3A 6085 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 945 93.09 202/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 3 8 8 13 8.8.13 [1.17.38.11] CARGLAVILGGFDYW in-frame + ccctggattcaccttcagtagttatagcatgcactgggtccgccaggctccagggaagggactggagtgggtctcctccattagtagtaatagtgcctacacagaccacgcagactcagtgaagggccgattcaccatctccagagacaacgacaagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagggctagcagtgattcttggaggatttgactactggggccagggaaacctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccctggcaccctcctccaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 86 88 [287, 288] t76>c,S26>P(+ - -)|c108>t|a118>c,N40>H(- - -)|g147>a|a162>c|g176>a,S59>N(- - -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>c,S63>A(- + -)|t194>c,I65>T(- - -)|t196>g,Y66>D(- - -)|t199>c,Y67>H(+ - -)|c248>a,A83>D(- - -)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)| t76>c,S26>P(+ - -)| c108>t| a118>c,N40>H(- - -)|g147>a|a162>c| g176>a,S59>N(- - -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>c,S63>A(- + -)|t194>c,I65>T(- - -)| t196>g,Y66>D(- - -)|t199>c,Y67>H(+ - -)|c248>a,A83>D(- - -)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)| a320>g| 319 (320) 224 (225) 209 15 (16) 3 (4) 12 2 (3) 2 1 4 2 1 0 0 1 1 0 1 78 4 3 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 0 0 2 0 0 0 0 0 0 0 30 24 19 5 0 5 1 1 0 2 0 0 0 0 1 0 0 0 117 114 109 5 0 5 1 0 0 1 0 1 0 0 0 1 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ccct ggattcaccttcagtagttatagc atgcactgggtccgccaggctccagggaagggactggagtgggtctcctcc attagtagtaatagtgcctacaca gaccacgcagactcagtgaagggccgattcaccatctccagagacaacgacaagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggctagcagtgattcttggaggatttgactac ARGLAVILGGFDY IGHV3-21 IGHD3-22 IGHJ4 ggattcaccttcagtagttatagc atgcactgggtccgccaggctccagggaagggactggagtgggtctcctcc attagtagtaatagtgcctacaca gaccacgcagactcagtgaagggccgattcaccatctccagagacaacgacaagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggctagcagtgattcttggaggatttgactac IGG1 14 213 8 6 3 6 5 8 2 1 6 5 JY8QFUQ01A4IGN 3917 productive Homsap IGHV3-7*01 F 959 93.58 204/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARETLPLGVGGFDNW in-frame + cctctggattcacatttagtaattattggatgatctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaaccaagatggaggtgacatggcctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactctctgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgtgcgagagagacactcccccttggtgttgggggttttgacaactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggcctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [292] c87>a|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g119>t,S40>I(- - -)|g171>c,K57>N(+ - -)|a187>g,S63>G(+ + -)|g192>c,E64>D(+ - +)|a194>t,K65>M(- + -)|a195>g,K65>M(- + -)|t196>g,Y66>A(- - -)|a197>c,Y66>A(- - -)|a258>t|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| c87>a|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g119>t,S40>I(- - -)| g171>c,K57>N(+ - -)|a187>g,S63>G(+ + -)|g192>c,E64>D(+ - +)|a194>t,K65>M(- + -)|a195>g,K65>M(- + -)| t196>g,Y66>A(- - -)|a197>c,Y66>A(- - -)|a258>t|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 226 212 14 2 12 2 3 1 0 1 1 2 0 2 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 0 0 1 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 30 24 19 5 0 5 2 0 0 0 0 0 1 0 2 0 0 0 117 114 109 5 1 4 0 2 0 0 1 0 1 0 0 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacatttagtaattattgg atgatctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaccaagatggaggtgacatg gcctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactctctgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgt gcgagagagacactcccccttggtgttgggggttttgacaac ARETLPLGVGGFDN IGHV3-7 IGHD3-10 IGHJ4 ggattcacatttagtaattattgg atgatctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaccaagatggaggtgacatg gcctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactctctgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgt gcgagagagacactcccccttggtgttgggggttttgacaac IGG2 14 213 6 8 4 8 2 6 1 1 5 7 JY8QFUQ01A4IHW 19868 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 919 89.47 204/228 nt NA Homsap IGHJ6*02 F (see comment) 166 74.19 46/62 nt Homsap IGHD3-10*01 F 3 8 10 15 8.10.15 [3.17.38.11] CVTGVKASGGAHAMDVW in-frame - Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) gcagcctctggattcagtttcagagacgcctggatgaattgggtccgccagtctccagggaaggggctggagtgggttggccgtgttaaaaggaaaactgatggtgggacgtcggaatatgctgcattcgtgaaaggcagattcaccatctcaagagatgattcagaaaacacactgtatctgcaaatgaacagcctggaaatcgacgacacagctgtgtatcgttgtgtcacaggagttaaggcttcggggggggctcacgctatggacgtctggggccaagggaccgcggtcatcgtctcctcagcatcccccgaccagcccccaaggtcttcccgctgagcctctgcagcagcccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 79 79 [307, 306] c86>g,T29>S(+ - +)|t105>a,S35>R(- - -)|a106>g,N36>D(+ + -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g133>t,A45>S(- + -)|a166>g,I56>V(+ - +)|c174>g,S58>R(- - -)|a192>g|a193>t,T65>S(+ - +)|a195>g,T65>S(+ - +)|c198>a,D66>E(+ - +)|c201>t|c208>t,P70>F(- - -)|c209>t,P70>F(- - -)|a250>g,K84>E(+ - -)|g258>a|a283>g,K95>E(+ - -)|c287>t,T96>I(- - -)|g291>c,E97>D(+ - +)|c300>t|t307>c,Y103>R(- - -)|a308>g,Y103>R(- - -)|c309>t,Y103>R(- - -)|a313>g,T105>V(- - -)|c314>t,T105>V(- - -)| c86>g,T29>S(+ - +)|t105>a,S35>R(- - -)|a106>g,N36>D(+ + -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g133>t,A45>S(- + -)| a166>g,I56>V(+ - +)|c174>g,S58>R(- - -)|a192>g|a193>t,T65>S(+ - +)|a195>g,T65>S(+ - +)| c198>a,D66>E(+ - +)|c201>t|c208>t,P70>F(- - -)|c209>t,P70>F(- - -)|a250>g,K84>E(+ - -)|g258>a|a283>g,K95>E(+ - -)|c287>t,T96>I(- - -)|g291>c,E97>D(+ - +)|c300>t|t307>c,Y103>R(- - -)|a308>g,Y103>R(- - -)|c309>t,Y103>R(- - -)| a313>g,T105>V(- - -)|c314>t,T105>V(- - -)|a320>g| 319 (320) 235 (236) 209 26 (27) 4 (5) 22 8 (9) 2 8 1 0 1 1 1 1 2 1 0 78 9 9 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 0 0 0 0 0 0 1 0 1 0 0 51 51 48 3 0 3 0 1 1 0 0 0 0 0 0 0 1 0 30 30 25 5 1 4 3 0 0 0 0 0 1 0 0 1 0 0 117 114 101 13 3 10 3 1 6 1 0 1 0 0 1 0 0 0 7 (8) 7 5 2 (3) 0 (1) 2 1 (2) 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| gcagcctct ggattcagtttcagagacgcctgg atgaattgggtccgccagtctccagggaaggggctggagtgggttggccgt gttaaaaggaaaactgatggtgggacgtcg gaatatgctgcattcgtgaaaggcagattcaccatctcaagagatgattcagaaaacacactgtatctgcaaatgaacagcctggaaatcgacgacacagctgtgtatcgttgt gtcacaggagttaaggcttcggggggggctcacgctatggacgtc VTGVKASGGAHAMDV IGHV3-15 IGHD3-10 IGHJ6 ggattcagtttcagagacgcctgg atgaattgggtccgccagtctccagggaaggggctggagtgggttggccgt gttaaaaggaaaactgatggtgggacgtcg gaatatgctgcattcgtgaaaggcagattcaccatctcaagagatgattcagaaaacacactgtatctgcaaatgaacagcctggaaatcgacgacacagctgtgtatcgttgt gtcacaggagttaaggcttcggggggggctcacgctatggacgtc IGA1 24 219 17 7 9 14 8 10 3 1 13 7 JY8QFUQ01A4J2Q 10258 productive (see comment) Homsap IGHV3-66*02 F, or Homsap IGHV3-66*03 F 873 89.4 194/217 nt 88.94 193/217 nt Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD6-25*01 F 1 8 7 11 8.7.11 [2.17.38.11] CARDGRGGDLDSW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 63 to 63 in the user submitted sequence), (cause frameshift); agtgtctgggttctccgtcagtttcaactacatgagctgggtccgccaggctccagggaaggGggctggagtgggtctcagttatctatgccgatggaagtacattctatgcagactccgtgaagggccgattcatcatctccagagacaattcaaagaacacgctcaatcttcaaatgaatagtttgagagttgacgacacggctgtgtattactgtgcgagagatgggcgcggcggtgaccttgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [282] c74>t,A25>V(+ - +)|c75>g,A25>V(+ - +)|a81>g|a85>t,T29>S(+ - +)|a106>t,S36>F(- - -)|g107>t,S36>F(- - -)|t168>c|a172>g,S58>A(- + -)|g173>c,S58>A(- + -)|g176>a,G59>D(- - -)|t189>a|c192>t|a197>t,Y66>F(- + -)|c201>t|c230>t,T77>I(- - -)|c249>a|g261>c|t262>a,Y88>N(- - -)|c276>t|c279>t|c280>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| c74>t,A25>V(+ - +)|c75>g,A25>V(+ - +)| a81>g|a85>t,T29>S(+ - +)|a106>t,S36>F(- - -)|g107>t,S36>F(- - -)| t168>c|a172>g,S58>A(- + -)|g173>c,S58>A(- + -)|g176>a,G59>D(- - -)|t189>a|c192>t| a197>t,Y66>F(- + -)|c201>t|c230>t,T77>I(- - -)|c249>a|g261>c|t262>a,Y88>N(- - -)|c276>t|c279>t|c280>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| 318 223 200 23 10 13 2 1 8 1 0 1 3 2 3 1 1 0 78 7 5 2 0 2 0 0 1 0 0 0 0 0 0 1 0 0 36 24 20 4 1 3 1 0 0 0 0 0 2 0 0 0 1 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 15 6 3 3 1 1 1 1 0 0 0 1 1 0 0 0 117 114 103 11 6 5 0 0 6 0 0 1 1 1 2 0 0 0 6 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| agtgtct gggttctccgtcagtttcaactac atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagtt atctatgccgatggaagtaca ttctatgcagactccgtgaagggccgattcatcatctccagagacaattcaaagaacacgctcaatcttcaaatgaatagtttgagagttgacgacacggctgtgtattactgt gcgagagatgggcgcggcggtgaccttgactcc ARDGRGGDLDS IGHV3-66 IGHD6-25 IGHJ4 gggttctccgtcagtttcaactac atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagtt atctatgccgatggaagtaca ttctatgcagactccgtgaagggccgattcatcatctccagagacaattcaaagaacacgctcaatcttcaaatgaatagtttgagagttgacgacacggctgtgtattactgt gcgagagatgggcgcggcggtgaccttgactcc IGA1 21 210 11 10 8 13 3 8 6 4 5 6 JY8QFUQ01A4JI5 5205 productive Homsap IGHV3-48*02 F 864 88.94 193/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-16*01 F 2 8 8 9 8.8.9 [1.17.38.11] CAWGPNGADYW in-frame + ctctggattcaccttcggtaactcaaccatgaactgggtccgccaggctccagggaaggggctggaatggctgtcatatcttagtagtggtggtgatgtcaaatactacgcaggctctctgaagggccgattcaccatctccagagacagtgccaggaactcactgtatctgcaaatgaacagcctgacagacgaggacacggccgtatattactgtgcatgggggcccaacggcgccgactactggggccagggaatcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 86 94 [276] a103>g,S35>G(+ + -)|g107>a,S36>N(- - -)|a110>c,Y37>S(+ - -)|t111>a,Y37>S(+ - -)|g113>c,S38>T(+ - +)|g153>a|g157>c,V53>L(+ - +)|t159>g,V53>L(+ - +)|c165>t|a166>c,I56>L(+ + +)|a175>g,S59>G(+ + -)|a184>g,S62>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)|t194>a,I65>K(- + -)|a206>g,D69>G(- - -)|g211>c,V71>L(+ - +)|a245>g,N82>S(- - -)|a251>g,K84>R(+ + +)|g284>c,R95>T(- - -)|t300>c|g303>a| a103>g,S35>G(+ + -)|g107>a,S36>N(- - -)|a110>c,Y37>S(+ - -)|t111>a,Y37>S(+ - -)|g113>c,S38>T(+ - +)| g153>a|g157>c,V53>L(+ - +)|t159>g,V53>L(+ - +)|c165>t| a166>c,I56>L(+ + +)|a175>g,S59>G(+ + -)|a184>g,S62>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)|t194>a,I65>K(- + -)| a206>g,D69>G(- - -)|g211>c,V71>L(+ - +)|a245>g,N82>S(- - -)|a251>g,K84>R(+ + +)|g284>c,R95>T(- - -)|t300>c|g303>a| g315>a|a316>t,R106>W(- - -)|a318>g,R106>W(- - -)|a320>g| 314 (320) 219 (225) 195 (197) 24 (28) 4 (6) 20 (22) 8 (10) 4 (5) 2 1 2 0 0 (1) 2 4 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 1 0 0 1 0 0 1 1 0 0 0 51 51 47 4 2 2 0 1 1 0 0 0 0 0 1 0 0 1 30 24 16 8 0 8 4 1 1 0 1 0 0 1 0 0 0 0 117 114 107 7 2 5 3 1 0 1 0 0 0 0 2 0 0 0 2 (8) 2 2 (4) 0 (4) 0 (2) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ctct ggattcaccttcggtaactcaacc atgaactgggtccgccaggctccagggaaggggctggaatggctgtcatat cttagtagtggtggtgatgtcaaa tactacgcaggctctctgaagggccgattcaccatctccagagacagtgccaggaactcactgtatctgcaaatgaacagcctgacagacgaggacacggccgtatattactgt gcatgggggcccaacggcgccgactac AWGPNGADY IGHV3-48 IGHD3-16 IGHJ4 ggattcaccttcggtaactcaacc atgaactgggtccgccaggctccagggaaggggctggaatggctgtcatat cttagtagtggtggtgatgtcaaa tactacgcaggctctctgaagggccgattcaccatctccagagacagtgccaggaactcactgtatctgcaaatgaacagcctgacagacgaggacacggccgtatattactgt gcatgggggcccaacggcgccgactac IGG1 24 213 15 9 6 10 9 14 4 0 7 13 JY8QFUQ01A4KIH 7697 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 958 93.98 203/216 nt NA Homsap IGHJ6*02 F 265 91.94 57/62 nt Homsap IGHD1-20*01 F 1 8 8 19 8.8.19 [1.17.38.11] CANASPRITGTYFYYYGMDVW in-frame - tctggattcatcttcagtagctatgccatgaattgggtccgccagactccagggaaggggctggagtgggtctcagccattagtggtagtggtggtaacacatactacgcagactccgtgaagggccggttcaccgtctccagagacaattccaacaacacgctgtatctgcaattggacagcctgcgagccgaggacacggccgtatattactgtgcgaatgcctccccccgtataactggaacgtatttctactactacggtatggacgtctggggccaagggaccacggtcagcgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [305] c86>t,T29>I(- - -)|t90>c|c105>t|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g133>a,A45>T(- - -)|t165>c|g191>a,S64>N(- - -)|a232>g,I78>V(+ - +)|g252>c,K84>N(+ - -)|a271>t,M91>L(+ + -)|a274>g,N92>D(+ + -)|a283>c| c86>t,T29>I(- - -)|t90>c|c105>t| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g133>a,A45>T(- - -)|t165>c| g191>a,S64>N(- - -)| a232>g,I78>V(+ - +)|g252>c,K84>N(+ - -)|a271>t,M91>L(+ + -)|a274>g,N92>D(+ + -)|a283>c| a318>t,K106>N(+ - -)|a320>c| 317 (320) 221 (224) 208 (209) 13 (15) 4 (5) 9 (10) 2 3 3 2 1 (2) 0 1 (2) 0 1 0 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 2 1 0 0 0 0 0 0 0 0 51 51 47 4 1 3 0 2 1 1 0 0 0 0 0 0 0 0 30 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 117 114 109 5 1 4 2 0 0 0 1 0 1 0 1 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcatcttcagtagctatgcc atgaattgggtccgccagactccagggaaggggctggagtgggtctcagcc attagtggtagtggtggtaacaca tactacgcagactccgtgaagggccggttcaccgtctccagagacaattccaacaacacgctgtatctgcaattggacagcctgcgagccgaggacacggccgtatattactgt gcgaatgcctccccccgtataactggaacgtatttctactactacggtatggacgtc ANASPRITGTYFYYYGMDV IGHV3-23 IGHD1-20 IGHJ6 ggattcatcttcagtagctatgcc atgaattgggtccgccagactccagggaaggggctggagtgggtctcagcc attagtggtagtggtggtaacaca tactacgcagactccgtgaagggccggttcaccgtctccagagacaattccaacaacacgctgtatctgcaattggacagcctgcgagccgaggacacggccgtatattactgt gcgaatgcctccccccgtataactggaacgtatttctactactacggtatggacgtc IGG1 13 213 10 3 6 7 4 6 2 2 7 2 JY8QFUQ01A4KIW 20908 productive Homsap IGHV3-48*01 F, or Homsap IGHV3-48*03 F 806 85.78 187/218 nt NA Homsap IGHJ4*02 F (see comment) 186 87.5 42/48 nt Homsap IGHD1-1*01 F 3 8 8 10 8.8.10 [1.17.38.11] CVRDLNWSFDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) cctctggattcacctttactagttacagtttcaactgggtccgccaggctccagggaaggcgctggagtggatttcatacatcactatcaatggtaatgacaagttctacgcaggctctgtgaagggccgattcgccgtctccagagacgatgccaagaattctctgtatctgcaaatgagcagcctgagagccgaagacacgggtgtttattactgtgtgagggacctcaactggtcttttgactcctggggccagggaaccctggtcaccgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccctaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccca NA IGA2 71 95 [279, 281] c90>t|g104>c,S35>T(+ - +)|c108>t|t111>c|c114>t|a115>t,M39>F(+ - -)|g117>c,M39>F(+ - -)|g146>c,G49>A(- + -)|g157>a,V53>I(+ - +)|t168>c|g170>c,S57>T(+ - +)|g173>t,S58>I(- - -)|t174>c,S58>I(- - -)|g176>a,S59>N(- - -)|a184>g,S62>G(+ + -)|g188>a,S63>N(- - -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|t194>a,I65>K(- + -)|a195>g,I65>K(- + -)|a197>t,Y66>F(- + -)|a206>g,D69>G(- - -)|a229>g,T77>A(- - -)|a232>g,I78>V(+ - +)|a244>g,N82>D(+ + -)|c255>t|a258>t|a275>g,N92>S(- - -)|g291>a|c299>g,A100>G(- + -)|g303>t|c314>t,A105>V(+ - +)|a318>g| c90>t|g104>c,S35>T(+ - +)|c108>t|t111>c|c114>t| a115>t,M39>F(+ - -)|g117>c,M39>F(+ - -)|g146>c,G49>A(- + -)|g157>a,V53>I(+ - +)| t168>c|g170>c,S57>T(+ - +)|g173>t,S58>I(- - -)|t174>c,S58>I(- - -)|g176>a,S59>N(- - -)|a184>g,S62>G(+ + -)|g188>a,S63>N(- - -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|t194>a,I65>K(- + -)|a195>g,I65>K(- + -)| a197>t,Y66>F(- + -)|a206>g,D69>G(- - -)|a229>g,T77>A(- - -)|a232>g,I78>V(+ - +)|a244>g,N82>D(+ + -)|c255>t|a258>t|a275>g,N92>S(- - -)|g291>a|c299>g,A100>G(- + -)|g303>t| c314>t,A105>V(+ - +)|a318>g| 320 226 193 33 10 23 9 4 5 3 0 1 3 1 4 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 4 1 0 0 3 1 0 0 0 0 1 0 0 0 51 51 47 4 0 4 0 1 0 0 0 0 1 0 2 0 0 0 30 24 13 11 1 10 3 2 0 2 0 1 0 1 1 0 1 0 117 114 103 11 4 7 5 1 1 0 0 0 2 0 0 1 1 0 8 8 6 2 1 1 1 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttactagttacagt ttcaactgggtccgccaggctccagggaaggcgctggagtggatttcatac atcactatcaatggtaatgacaag ttctacgcaggctctgtgaagggccgattcgccgtctccagagacgatgccaagaattctctgtatctgcaaatgagcagcctgagagccgaagacacgggtgtttattactgt gtgagggacctcaactggtcttttgactcc VRDLNWSFDS IGHV3-48 IGHD1-1 IGHJ4 ggattcacctttactagttacagt ttcaactgggtccgccaggctccagggaaggcgctggagtggatttcatac atcactatcaatggtaatgacaag ttctacgcaggctctgtgaagggccgattcgccgtctccagagacgatgccaagaattctctgtatctgcaaatgagcagcctgagagccgaagacacgggtgtttattactgt gtgagggacctcaactggtcttttgactcc IGA2 31 213 19 12 8 16 11 15 4 5 11 11 JY8QFUQ01A4LR9 20672 productive (see comment) Homsap IGHV4-61*02 F 987 94.55 208/220 nt 93.64 206/220 nt Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD3-10*01 F 3 10 7 11 10.7.11 [1.17.37.11] CATVARRGAFDIW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 13 to 13 in the user submitted sequence), (cause frameshift); in FR3-IMGT, from codon 96 of V-REGION: 1 nucleotides (from position 195 in the user submitted sequence), (cause frameshift) tctctggtgggtGccatcagcagtactagttactactggagctggatccggcagcccgccgggaagggactggagtggatggggcgtatctataccagtgggatcaccaactacaacccctccctcgagagtcgagtcaccttttcagtggacacgtccaagaaccagttctccctgaagctgaagtctgtgaccccgcagacacggccgtttattactgtgcgacagtggcgcgccggggggcttttgatatctggggccaagggacactggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG3 93 100 [286] c84>g|g103>a,G35>T(+ - -)|g104>c,G35>T(+ - -)|t159>g,I53>M(+ + -)|g191>t,S64>I(- - -)|a214>g,K72>E(+ - -)|a232>t,I78>F(+ - -)|a234>t,I78>F(+ - -)|a240>g|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g303>t|g317>c,R106>T(- - -)| c84>g|g103>a,G35>T(+ - -)|g104>c,G35>T(+ - -)| t159>g,I53>M(+ + -)| g191>t,S64>I(- - -)| a214>g,K72>E(+ - -)|a232>t,I78>F(+ - -)|a234>t,I78>F(+ - -)|a240>g|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g303>t| g317>c,R106>T(- - -)|a320>t| 319 (320) 227 (228) 214 13 (14) 3 (4) 10 2 2 0 0 0 0 2 (3) 0 2 2 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 1 2 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 30 21 20 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 117 113 106 7 2 5 2 1 0 0 0 0 2 0 0 1 1 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 0 0 0 0 0 (1) 0 1 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgggtccatcagcagtactagttactac tggagctggatccggcagcccgccgggaagggactggagtggatggggcgt atctataccagtgggatcacc aactacaacccctccctcgagagtcgagtcaccttttcagtggacacgtccaagaaccagttctccctgaagctgaagtctgtgaccccgcagacacggccgtttattactgt gcgacagtggcgcgccggggggcttttgatatc ATVARRGAFDI IGHV4-61 IGHD3-10 IGHJ3 ggtgggtccatcagcagtactagttactac tggagctggatccggcagcccgccgggaagggactggagtggatggggcgt atctataccagtgggatcacc aactacaacccctccctcgagagtcgagtcaccttttcagtggacacgtccaagaaccagttctccctgaagctgaagtctgtgaccccgcagacacggccgtttattactgt gcgacagtggcgcgccggggggcttttgatatc IGG3 12 215 4 8 2 7 2 5 2 1 6 3 JY8QFUQ01A4LRG 17814 productive (see comment) Homsap IGHV1-2*02 F 930 92.99 199/214 nt 92.52 198/214 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-19*01 F 1 8 8 12 8.8.12 [0.17.38.11] CARGPVSGWYTDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 57 of V-REGION: 'AACCCC' (6 nucleotides at position 80 to 85 in the user submitted sequence), (do not cause frameshift); tggatacaccttcaccgtctactatctattctgggtgcgacgggcccctggacaagggcttgagtggatgggatggatcAACCCCaaccctaagagtggtgacacacactatgcaccgaaattccagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggaactgaataggctgagatctgacgacacggccgtgtattactgtgcgagaggccctgtcagtggctggtacactgactactggggccagggaaccctggtcaacgtctcatcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaaggcatggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 84 [287, 288] g107>t,G36>V(- - -)|a115>c,M39>L(+ + -)|g117>a,M39>L(+ + -)|c118>t,H40>F(- - -)|a119>t,H40>F(- - -)|a131>g,Q44>R(+ - -)|c177>g,N59>K(+ - -)|g191>a,G64>D(- - -)|a196>c,N66>H(- - -)|a206>c,Q69>P(- - -)|g210>a|t213>c|g270>a|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)| g107>t,G36>V(- - -)| a115>c,M39>L(+ + -)|g117>a,M39>L(+ + -)|c118>t,H40>F(- - -)|a119>t,H40>F(- - -)|a131>g,Q44>R(+ - -)| c177>g,N59>K(+ - -)|g191>a,G64>D(- - -)| a196>c,N66>H(- - -)|a206>c,Q69>P(- - -)|g210>a|t213>c|g270>a|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)| a320>g| 319 (320) 221 (222) 206 15 (16) 3 (4) 12 1 (2) 5 2 1 3 0 1 0 0 1 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 51 51 46 5 0 5 1 1 1 0 1 0 1 0 0 0 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 0 1 0 0 117 114 107 7 3 4 0 3 1 1 2 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatacaccttcaccgtctactat ctattctgggtgcgacgggcccctggacaagggcttgagtggatgggatgg atcaaccctaagagtggtgacaca cactatgcaccgaaattccagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggaactgaataggctgagatctgacgacacggccgtgtattactgt gcgagaggccctgtcagtggctggtacactgactac ARGPVSGWYTDY IGHV1-2 IGHD6-19 IGHJ4 ggatacaccttcaccgtctactat ctattctgggtgcgacgggcccctggacaagggcttgagtggatgggatgg atcaaccctaagagtggtgacaca cactatgcaccgaaattccagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggaactgaataggctgagatctgacgacacggccgtgtattactgt gcgagaggccctgtcagtggctggtacactgactac IGA2 15 213 9 6 7 9 2 6 3 0 9 3 JY8QFUQ01A4LRU 13600 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 860 88.53 193/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-15*01 F 3 8 8 14 8.8.14 [1.17.38.11] CAKEEGATGVYYVDHW in-frame + cctctggattcacctttaccacctccgccatggcctgggtccgccaggttccagggaaggggctggagtgggtctcaactattagacctagtggtgagagaacctactacgcagagtccgtgaggggccgcttcaccatctccagagacaattccgagaacacgttgtatctacaactgaacaacctgagagtcgaggacacggccatatattactgtgcgaaggaggaaggtgctactggagtctattatgttgaccactggggccagggaacccgggtcaccgtctcctcatcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [292] g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|g163>a,A55>T(- - -)|t171>a,S57>R(- - -)|g172>c,G58>P(+ - -)|g173>c,G58>P(+ - -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)|a195>c|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|g225>c|a250>g,K84>E(+ - -)|c259>t|g267>a|a271>c,M91>L(+ + -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|a318>g| g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|g163>a,A55>T(- - -)| t171>a,S57>R(- - -)|g172>c,G58>P(+ - -)|g173>c,G58>P(+ - -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)|a195>c| c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|g225>c|a250>g,K84>E(+ - -)|c259>t|g267>a|a271>c,M91>L(+ + -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)| a318>g| 320 226 200 26 5 21 4 5 3 1 3 1 0 1 6 1 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 1 1 0 0 0 2 0 0 0 51 51 47 4 0 4 1 1 1 0 0 0 0 0 1 0 0 0 30 24 17 7 1 6 0 1 0 0 1 1 0 1 2 0 0 1 117 114 104 10 3 7 2 3 2 0 1 0 0 0 1 1 0 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttaccacctccgcc atggcctgggtccgccaggttccagggaaggggctggagtgggtctcaact attagacctagtggtgagagaacc tactacgcagagtccgtgaggggccgcttcaccatctccagagacaattccgagaacacgttgtatctacaactgaacaacctgagagtcgaggacacggccatatattactgt gcgaaggaggaaggtgctactggagtctattatgttgaccac AKEEGATGVYYVDH IGHV3-23 IGHD2-15 IGHJ4 ggattcacctttaccacctccgcc atggcctgggtccgccaggttccagggaaggggctggagtgggtctcaact attagacctagtggtgagagaacc tactacgcagagtccgtgaggggccgcttcaccatctccagagacaattccgagaacacgttgtatctacaactgaacaacctgagagtcgaggacacggccatatattactgt gcgaaggaggaaggtgctactggagtctattatgttgaccac IGG2 25 213 12 13 8 16 4 9 3 1 11 10 JY8QFUQ01A4MB9 12095 productive (see comment) Homsap IGHV4-61*07 F (see comment) 775 84.02 184/219 nt 83.56 183/219 nt Homsap IGHJ3*02 F (see comment) 178 84 42/50 nt Homsap IGHD3-22*01 F 2 10 7 13 10.7.13 [1.17.38.11] CARDGNRGYYSFETW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (84,02% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*01 and Homsap_IGHJ4*03 and Homsap_IGHJ5*01 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 41 of V-REGION: 'T' (1 nucleotides at position 42 to 42 in the user submitted sequence), (cause frameshift); gtctggtggctccgtcagcagtggaaattcctactggacctTggatccgccagtcccccgagaagggactggagtggcttgcatatattcgaaacactgggacaaccaactacaacccctccctcaagagtagactcaccatgtctctggacatgtctaggaatcagttctccctgaggctgaacgatgtgaccgctgcggacacggccatatattactgtgcgagagacggcaatagaggttattactcttttgaaacctggggccgagggacattggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [292] t105>a|g107>a,S36>N(- - -)|a110>c,Y37>S(+ - -)|g119>c,S40>T(+ - +)|g129>c|c133>t,P45>S(+ - -)|a138>c|g140>a,G47>E(- - -)|a157>c,I53>L(+ + +)|g161>c,G54>A(- + -)|g162>a,G54>A(- + -)|c168>t|t169>c,Y57>R(- - -)|a170>g,Y57>R(- - -)|t171>a,Y57>R(- - -)|t172>a,Y58>N(- - -)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)|c223>a|g226>c,V76>L(+ - +)|a234>g,I78>M(+ + -)|a237>t|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|c245>t,T82>M(- - -)|c249>t|a251>g,K84>R(+ + +)|c255>t|a269>g,K90>R(+ + +)|g275>a,S92>N(- - -)|t277>g,S93>D(- - -)|c278>a,S93>D(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| t105>a|g107>a,S36>N(- - -)|a110>c,Y37>S(+ - -)| g119>c,S40>T(+ - +)|g129>c|c133>t,P45>S(+ - -)|a138>c|g140>a,G47>E(- - -)|a157>c,I53>L(+ + +)|g161>c,G54>A(- + -)|g162>a,G54>A(- + -)| c168>t|t169>c,Y57>R(- - -)|a170>g,Y57>R(- - -)|t171>a,Y57>R(- - -)|t172>a,Y58>N(- - -)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)| c223>a|g226>c,V76>L(+ - +)|a234>g,I78>M(+ + -)|a237>t|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|c245>t,T82>M(- - -)|c249>t|a251>g,K84>R(+ + +)|c255>t|a269>g,K90>R(+ + +)|g275>a,S92>N(- - -)|t277>g,S93>D(- - -)|c278>a,S93>D(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| c319>g| 318 (320) 226 (228) 191 (192) 35 (36) 8 (9) 27 5 6 5 1 3 3 1 3 7 0 (1) 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 1 2 0 1 0 0 1 0 0 1 0 0 0 0 51 51 43 8 2 6 0 2 1 0 2 0 0 0 3 0 0 0 30 21 13 8 1 7 1 0 1 1 0 1 0 2 2 0 0 0 117 114 98 16 4 12 4 3 3 0 0 2 1 0 2 0 0 1 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 0 (1) 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtct ggtggctccgtcagcagtggaaattcctac tggacctggatccgccagtcccccgagaagggactggagtggcttgcatat attcgaaacactgggacaacc aactacaacccctccctcaagagtagactcaccatgtctctggacatgtctaggaatcagttctccctgaggctgaacgatgtgaccgctgcggacacggccatatattactgt gcgagagacggcaatagaggttattactcttttgaaacc ARDGNRGYYSFET IGHV4-61 IGHD3-22 IGHJ3 ggtggctccgtcagcagtggaaattcctac tggacctggatccgccagtcccccgagaagggactggagtggcttgcatat attcgaaacactgggacaacc aactacaacccctccctcaagagtagactcaccatgtctctggacatgtctaggaatcagttctccctgaggctgaacgatgtgaccgctgcggacacggccatatattactgt gcgagagacggcaatagaggttattactcttttgaaacc IGG1 35 216 17 18 11 21 6 14 6 2 18 9 JY8QFUQ01A4MZ9 5355 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 855 88.48 192/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-10*01 F 2 8 8 15 8.8.15 [1.17.38.11] CVKGWNYHSATFGFDYW in-frame + ctctggattcacctttagtgactatgccataagctgggtccgccaggctccaggaaaggggctggagtgggtctcctctattagtgctactggtggaattacatcttacgcagattccgtgaagggccggttcaccattttcagagacaactccaaagacacgctgtatctgcaaatgggcagcctgagagacgaggacacggccatttattactgtgtgaagggttggaattaccatagtgcaacctttgggtttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [293] c105>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g117>a,M39>I(+ + -)|g141>a|a162>c|g163>t,A55>S(- + -)|g173>c,G58>A(- + -)|g176>c,S59>T(+ - +)|t189>a|g191>t,S64>I(- - -)|c192>t,S64>I(- - -)|a197>c,Y66>S(+ - -)|c198>t,Y66>S(+ - -)|c207>t|c234>t|c236>t,S79>F(- - -)|t246>c|g252>a|a253>g,N85>D(+ + -)|a274>g,N92>G(- - -)|a275>g,N92>G(- - -)|c287>a,A96>D(- - -)|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)|c314>t,A105>V(+ - +)| c105>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g117>a,M39>I(+ + -)|g141>a|a162>c|g163>t,A55>S(- + -)| g173>c,G58>A(- + -)|g176>c,S59>T(+ - +)|t189>a|g191>t,S64>I(- - -)|c192>t,S64>I(- - -)| a197>c,Y66>S(+ - -)|c198>t,Y66>S(+ - -)|c207>t|c234>t|c236>t,S79>F(- - -)|t246>c|g252>a|a253>g,N85>D(+ + -)|a274>g,N92>G(- - -)|a275>g,N92>G(- - -)|c287>a,A96>D(- - -)|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| c314>t,A105>V(+ - +)|a318>g|a320>g| 317 (320) 222 (225) 196 (197) 26 (28) 8 (10) 18 4 (6) 5 7 1 2 1 1 1 2 0 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 1 1 1 0 0 0 0 0 0 0 0 0 51 51 47 4 2 2 0 2 0 0 1 0 0 0 0 0 1 0 30 24 19 5 1 4 0 0 1 0 0 0 0 1 2 0 1 0 117 114 101 13 4 9 3 2 4 1 1 1 1 0 0 0 0 0 5 (8) 5 4 (5) 1 (3) 0 (2) 1 0 (2) 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtgactatgcc ataagctgggtccgccaggctccaggaaaggggctggagtgggtctcctct attagtgctactggtggaattaca tcttacgcagattccgtgaagggccggttcaccattttcagagacaactccaaagacacgctgtatctgcaaatgggcagcctgagagacgaggacacggccatttattactgt gtgaagggttggaattaccatagtgcaacctttgggtttgactac VKGWNYHSATFGFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcacctttagtgactatgcc ataagctgggtccgccaggctccaggaaaggggctggagtgggtctcctct attagtgctactggtggaattaca tcttacgcagattccgtgaagggccggttcaccattttcagagacaactccaaagacacgctgtatctgcaaatgggcagcctgagagacgaggacacggccatttattactgt gtgaagggttggaattaccatagtgcaacctttgggtttgactac IGA1 25 213 16 9 11 16 5 9 6 2 11 6 JY8QFUQ01A4NWM 19606 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 927 92.17 200/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-18*01 F 1 8 8 12 8.8.12 [1.17.38.11] CAKGDSGIRPIDYW in-frame + ctctggattcacctctagcgcctataccatgagttgggtccgccaggctccagggaaggggctggagtgcgtctcagctattagtggtggtggtactagcacatactacgcagactccgtgaagggccggttcaccatctccagagacacttccaagaacacactgtctctgcacctgaacagcctgagagtcgaggacacggccatatatttctgtgcgaaaggtgattctggtataagacctattgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccccctgcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 93 88 [285] t89>c,F30>S(- - -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|g112>a,A38>T(- - -)|c120>t|g156>c,W52>C(+ - -)|a175>g,S59>G(+ + -)|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|a245>c,N82>T(- + -)|g258>a|a263>c,Y88>S(+ - -)|a270>c,Q90>H(- + -)|a271>c,M91>L(+ + -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|a308>t,Y103>F(- + -)| t89>c,F30>S(- - -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|g112>a,A38>T(- - -)| c120>t|g156>c,W52>C(+ - -)| a175>g,S59>G(+ + -)|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)| a245>c,N82>T(- + -)|g258>a|a263>c,Y88>S(+ - -)|a270>c,Q90>H(- + -)|a271>c,M91>L(+ + -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|a308>t,Y103>F(- + -)| a320>g| 319 (320) 224 (225) 207 17 (18) 2 (3) 15 2 (3) 4 2 1 4 0 1 0 3 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 0 1 0 0 0 0 1 0 0 0 51 51 49 2 1 1 0 0 1 0 0 0 0 0 1 0 0 0 30 24 21 3 0 3 1 1 0 0 0 0 0 0 1 0 0 0 117 114 106 8 1 7 0 2 1 0 4 0 1 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctctagcgcctatacc atgagttgggtccgccaggctccagggaaggggctggagtgcgtctcagct attagtggtggtggtactagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacacttccaagaacacactgtctctgcacctgaacagcctgagagtcgaggacacggccatatatttctgt gcgaaaggtgattctggtataagacctattgactac AKGDSGIRPIDY IGHV3-23 IGHD5-18 IGHJ4 ggattcacctctagcgcctatacc atgagttgggtccgccaggctccagggaaggggctggagtgcgtctcagct attagtggtggtggtactagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacacttccaagaacacactgtctctgcacctgaacagcctgagagtcgaggacacggccatatatttctgt gcgaaaggtgattctggtataagacctattgactac IGG2 17 213 9 8 6 9 3 8 2 0 8 7 JY8QFUQ01A4ORW 7383 productive Homsap IGHV3-9*01 F (see comment) 774 84.33 183/217 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD3-10*01 F 3 8 8 16 8.8.16 [1.17.38.11] CVKDMVAGQGLLSRFDHW in-frame + Low V-REGION identity (84,33% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctggattcgactttaaggaatatgccatacactgggtccggcaagttccaggaaagggcctggagtgggtcgcgggcatcaactggaatcggggcaaagcattgtatggggactctgtgaggggccgattcaccatctccagagacaacgcccagaactccgtgtctctgcaaatgaacagtctgaggcctgacgacacggccttgtatatctgtgtaaaagacatggtcgcgggtcagggactcttgtcacggtttgaccactggggccagggagtcctggtcaccgtctctccagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [297] a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|g103>a,D35>K(+ - -)|t105>g,D35>K(+ - -)|t108>a,D36>E(+ - +)|g117>a,M39>I(+ + -)|c134>t,A45>V(+ - +)|g141>a|t160>g,S54>A(- + -)|a162>g,S54>A(- + -)|t165>c|t168>c|g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|a184>c,S62>R(- - -)|t186>g,S62>R(- - -)|t189>c|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|a193>g,I65>A(+ - +)|t194>c,I65>A(+ - +)|g196>t,G66>L(- - -)|g197>t,G66>L(- - -)|c198>g,G66>L(- - -)|c203>g,A68>G(- + -)|a215>g,K72>R(+ + +)|a250>c,K84>Q(+ - -)|c259>g,L87>V(+ - +)|a263>c,Y88>S(+ - -)|a285>g|g286>c,A96>P(- - -)|g291>c,E97>D(+ - +)|t307>a,Y103>I(- - -)|a308>t,Y103>I(- - -)|c314>t,A105>V(+ - +)| a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|g103>a,D35>K(+ - -)|t105>g,D35>K(+ - -)|t108>a,D36>E(+ - +)| g117>a,M39>I(+ + -)|c134>t,A45>V(+ - +)|g141>a|t160>g,S54>A(- + -)|a162>g,S54>A(- + -)|t165>c| t168>c|g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|a184>c,S62>R(- - -)|t186>g,S62>R(- - -)|t189>c|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|a193>g,I65>A(+ - +)|t194>c,I65>A(+ - +)| g196>t,G66>L(- - -)|g197>t,G66>L(- - -)|c198>g,G66>L(- - -)|c203>g,A68>G(- + -)|a215>g,K72>R(+ + +)|a250>c,K84>Q(+ - -)|c259>g,L87>V(+ - +)|a263>c,Y88>S(+ - -)|a285>g|g286>c,A96>P(- - -)|g291>c,E97>D(+ - +)|t307>a,Y103>I(- - -)|a308>t,Y103>I(- - -)| c314>t,A105>V(+ - +)|t321>c| 320 (322) 225 (227) 190 (191) 35 (36) 5 (6) 30 5 5 2 5 (6) 3 2 1 2 2 3 2 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 1 0 0 0 1 0 1 0 0 0 1 51 51 45 6 2 4 1 2 1 1 0 0 0 0 0 0 0 1 30 24 14 10 2 8 1 2 0 4 1 1 0 0 0 0 0 1 117 114 101 13 1 12 2 0 0 0 2 0 1 1 2 3 2 0 8 (10) 8 7 (8) 1 (2) 0 (1) 1 0 0 1 0 (1) 0 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcgactttaaggaatatgcc atacactgggtccggcaagttccaggaaagggcctggagtgggtcgcgggc atcaactggaatcggggcaaagca ttgtatggggactctgtgaggggccgattcaccatctccagagacaacgcccagaactccgtgtctctgcaaatgaacagtctgaggcctgacgacacggccttgtatatctgt gtaaaagacatggtcgcgggtcagggactcttgtcacggtttgaccac VKDMVAGQGLLSRFDH IGHV3-9 IGHD3-10 IGHJ4 ggattcgactttaaggaatatgcc atacactgggtccggcaagttccaggaaagggcctggagtgggtcgcgggc atcaactggaatcggggcaaagca ttgtatggggactctgtgaggggccgattcaccatctccagagacaacgcccagaactccgtgtctctgcaaatgaacagtctgaggcctgacgacacggccttgtatatctgt gtaaaagacatggtcgcgggtcagggactcttgtcacggtttgaccac IGG1 34 213 16 18 6 15 10 19 3 2 16 13 JY8QFUQ01A4P7X 8895 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 1090 100 219/219 nt 99.09 217/219 nt Homsap IGHJ5*02 F 255 100 51/51 nt Homsap IGHD2-2*01 F 3 8 8 18 8.8.18 [2.16.38.11] CAKFMGVVVVPAAHNWFDPW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'G' (1 nucleotides at position 17 to 17 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 61 in the user submitted sequence), (cause frameshift) agcctctggattcaccGtttagcagctatgccatgagctgggtccgccaggctccagggaagggctggagtgggtctcagctattagtggtagtggtggtagcacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaaattcatgggagttgtagtagtaccagctgcccacaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [306] g319>t|a320>t| 318 (320) 225 (227) 225 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 50 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct ggattcacctttagcagctatgcc atgagctgggtccgccaggctccagggaagggctggagtgggtctcagct attagtggtagtggtggtagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaattcatgggagttgtagtagtaccagctgcccacaactggttcgacccc AKFMGVVVVPAAHNWFDP IGHV3-23 IGHD2-2 IGHJ5 ggattcacctttagcagctatgcc atgagctgggtccgccaggctccagggaagggctggagtgggtctcagct attagtggtagtggtggtagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaattcatgggagttgtagtagtaccagctgcccacaactggttcgacccc IGG2 0 212 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01A4PSY 10883 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 897 90 198/220 nt 89.55 197/220 nt Homsap IGHJ2*01 F 220 90.57 48/53 nt Homsap IGHD2-21*01 F 3 8 8 15 8.8.15 [2.17.38.11] CAKNRGSAMADWYFDFW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 58 of V-REGION: 'ATG' (3 nucleotides at position 90 to 92 in the user submitted sequence), (do not cause frameshift); agcctctggattcgactttagcggctttgccatgagctgggtccgccaggccccagggaaggggctggagtgggtctcaactattactaATGgtggtggtggtgtcgtactctacgcagactccgtgaagggccgattcaccatctccagagacaatgccaagaacacactgtatctgcagatgaacagcctgcgagccgaggacacggccgtttatttctgtgcgaaaaatagggggtcggcgatggctgactggtacttcgatttctggggccgcggcaacctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG2 100 100 [300] a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|a106>g,S36>G(+ + -)|a110>t,Y37>F(- + -)|t135>c|g163>a,A55>T(- - -)|g170>c,S57>T(+ - +)|g172>a,G58>S(+ + -)|a175>g,S59>G(+ + -)|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|t196>c,Y66>L(- - -)|a197>t,Y66>L(- - -)|g225>a|t247>g,S83>A(- + -)|g258>a|a270>g|a283>c|a303>t|a308>t,Y103>F(- + -)| a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|a106>g,S36>G(+ + -)|a110>t,Y37>F(- + -)| t135>c|g163>a,A55>T(- - -)| g170>c,S57>T(+ - +)|g172>a,G58>S(+ + -)|a175>g,S59>G(+ + -)|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)| t196>c,Y66>L(- - -)|a197>t,Y66>L(- - -)|g225>a|t247>g,S83>A(- + -)|g258>a|a270>g|a283>c|a303>t|a308>t,Y103>F(- + -)| g319>a| 318 (320) 226 (228) 204 (205) 22 (23) 6 (7) 16 6 4 (5) 1 2 1 1 4 0 1 0 1 1 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 2 0 0 0 0 1 1 0 0 0 0 0 51 51 49 2 1 1 0 1 0 1 0 0 0 0 0 0 0 0 30 24 17 7 0 7 3 1 1 0 0 0 0 0 1 0 1 0 117 114 105 9 5 4 1 2 0 1 1 0 3 0 0 0 0 1 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct ggattcgactttagcggctttgcc atgagctgggtccgccaggccccagggaaggggctggagtgggtctcaact attactagtggtggtggtgtcgta ctctacgcagactccgtgaagggccgattcaccatctccagagacaatgccaagaacacactgtatctgcagatgaacagcctgcgagccgaggacacggccgtttatttctgt gcgaaaaatagggggtcggcgatggctgactggtacttcgatttc AKNRGSAMADWYFDF IGHV3-23 IGHD2-21 IGHJ2 ggattcgactttagcggctttgcc atgagctgggtccgccaggccccagggaaggggctggagtgggtctcaact attactagtggtggtggtgtcgta ctctacgcagactccgtgaagggccgattcaccatctccagagacaatgccaagaacacactgtatctgcagatgaacagcctgcgagccgaggacacggccgtttatttctgt gcgaaaaatagggggtcggcgatggctgactggtacttcgatttc IGG2 22 213 13 9 5 8 8 14 6 0 5 11 JY8QFUQ01A4RGQ 18182 productive Homsap IGHV3-72*01 F 966 92.83 207/223 nt NA Homsap IGHJ5*02 F (see comment) 147 76.47 39/51 nt Homsap IGHD7-27*01 F 1 8 10 10 8.10.10 [1.17.38.11] CVRDPFSSRGHW in-frame - Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctggattcatcctcagtgactaccacatggagtgggtccgccaggctccagggaaggggctggagtgggttggccgtagtagaaagaaagctaatagttacagtacagaatatgccgcgtctgtgaaaggcagattctccatttcaagagatgattcaaagaactcactgtatctgcaaatgaacagcctgaaaatcgacgacacggccgtgtattattgtgtcagagaccccttctcaagtcggggccactggggccagggaaacctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] c86>t,T29>I(- - -)|t88>c,F30>L(+ - -)|c109>t,H37>Y(+ - -)|t112>c,Y38>H(+ - -)|c120>g,D40>E(+ - +)|c167>g,T56>S(+ - +)|c174>g,N58>K(+ - -)|c183>t|c191>g,T64>S(+ - +)|c192>t,T64>S(+ - +)|c201>t|a229>t,T77>S(+ - +)|c234>t|c287>t,T96>I(- - -)|g291>c,E97>D(+ - +)|c309>t|c314>t,A105>V(+ - +)|t315>c,A105>V(+ - +)| c86>t,T29>I(- - -)|t88>c,F30>L(+ - -)|c109>t,H37>Y(+ - -)|t112>c,Y38>H(+ - -)| c120>g,D40>E(+ - +)| c167>g,T56>S(+ - +)|c174>g,N58>K(+ - -)|c183>t|c191>g,T64>S(+ - +)|c192>t,T64>S(+ - +)| c201>t|a229>t,T77>S(+ - +)|c234>t|c287>t,T96>I(- - -)|g291>c,E97>D(+ - +)|c309>t| c314>t,A105>V(+ - +)|t315>c,A105>V(+ - +)| 320 231 213 18 4 14 0 0 9 3 0 0 1 0 1 4 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 2 2 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 30 25 5 1 4 0 0 2 0 0 0 0 0 0 3 0 0 117 114 108 6 3 3 0 0 4 0 0 0 1 0 1 0 0 0 8 8 6 2 0 2 0 0 1 1 0 0 0 0 0 0 0 0 ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,165-168(FR2-CDR2)|agct,177-180(CDR2)|aact,253-256(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tgct,312-315(FR3)| ctct ggattcatcctcagtgactaccac atggagtgggtccgccaggctccagggaaggggctggagtgggttggccgt agtagaaagaaagctaatagttacagtaca gaatatgccgcgtctgtgaaaggcagattctccatttcaagagatgattcaaagaactcactgtatctgcaaatgaacagcctgaaaatcgacgacacggccgtgtattattgt gtcagagaccccttctcaagtcggggccac VRDPFSSRGH IGHV3-72 IGHD7-27 IGHJ5 ggattcatcctcagtgactaccac atggagtgggtccgccaggctccagggaaggggctggagtgggttggccgt agtagaaagaaagctaatagttacagtaca gaatatgccgcgtctgtgaaaggcagattctccatttcaagagatgattcaaagaactcactgtatctgcaaatgaacagcctgaaaatcgacgacacggccgtgtattattgt gtcagagaccccttctcaagtcggggccac IGA1 16 219 10 6 8 13 2 3 3 1 4 8 JY8QFUQ01A4RRU 21032 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 986 94.95 207/218 nt 94.5 206/218 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD1-26*01 F 2 8 8 16 8.8.16 [1.17.38.11] CAKASPKWEFLRTTFDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 13 to 13 in the user submitted sequence), (cause frameshift); cctctggattcaGcctttcgcagctatgccatgacctgggtccgccaggctccagggaaggggctggaatgggtctcaactattagtggtagtgctggtagcacattctacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgacgacacggccatctacttctgtgcgaaggcgtcgccgaagtgggagtttctacgaaccacctttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [298] a103>c,S35>R(- - -)|g119>c,S40>T(+ - +)|g153>a|g163>a,A55>T(- - -)|g185>c,G62>A(- + -)|a197>t,Y66>F(- + -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|a303>c,V101>I(+ - +)|t306>c|a308>t,Y103>F(- + -)| a103>c,S35>R(- - -)| g119>c,S40>T(+ - +)|g153>a|g163>a,A55>T(- - -)| g185>c,G62>A(- + -)| a197>t,Y66>F(- + -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|a303>c,V101>I(+ - +)|t306>c|a308>t,Y103>F(- + -)| a318>g|a320>c| 317 (320) 223 (226) 212 (213) 11 (13) 2 (4) 9 0 (1) 3 0 1 2 (3) 0 2 0 3 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 51 51 48 3 1 2 0 2 0 0 0 0 0 0 1 0 0 0 30 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 117 114 108 6 1 5 0 1 0 1 1 0 2 0 1 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttcgcagctatgcc atgacctgggtccgccaggctccagggaaggggctggaatgggtctcaact attagtggtagtgctggtagcaca ttctacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgacgacacggccatctacttctgt gcgaaggcgtcgccgaagtgggagtttctacgaaccacctttgactac AKASPKWEFLRTTFDY IGHV3-23 IGHD1-26 IGHJ4 ggattcacctttcgcagctatgcc atgacctgggtccgccaggctccagggaaggggctggaatgggtctcaact attagtggtagtgctggtagcaca ttctacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgacgacacggccatctacttctgt gcgaaggcgtcgccgaagtgggagtttctacgaaccacctttgactac IGA1 11 213 4 7 3 6 1 5 2 0 7 2 JY8QFUQ01A4SGO 15223 productive (see comment) Homsap IGHV1-2*02 F 930 92.99 199/214 nt 92.06 197/214 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-19*01 F 1 8 8 12 8.8.12 [0.17.38.11] CARGPVSGWYTDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 44 of V-REGION: 'G' (1 nucleotides at position 42 to 42 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 57 of V-REGION: 'AACCCC' (6 nucleotides at position 81 to 86 in the user submitted sequence), (do not cause frameshift); tggatacaccttcaccgtctactatctattctgggtgcgacGgggcccctggacaagggcttgagtggatgggatggatcAACCCCaaccctaagagtggtgacacacactatgcaccgaaattccagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggaactgaataggctgagatctgacgacacggccgtgtattactgtgcgagaggccctgtcagtggctggtacactgactactggggccagggaaccctggtcaacgtctcatcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [288] g107>t,G36>V(- - -)|a115>c,M39>L(+ + -)|g117>a,M39>L(+ + -)|c118>t,H40>F(- - -)|a119>t,H40>F(- - -)|a131>g,Q44>R(+ - -)|c177>g,N59>K(+ - -)|g191>a,G64>D(- - -)|a196>c,N66>H(- - -)|a206>c,Q69>P(- - -)|g210>a|t213>c|g270>a|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)| g107>t,G36>V(- - -)| a115>c,M39>L(+ + -)|g117>a,M39>L(+ + -)|c118>t,H40>F(- - -)|a119>t,H40>F(- - -)|a131>g,Q44>R(+ - -)| c177>g,N59>K(+ - -)|g191>a,G64>D(- - -)| a196>c,N66>H(- - -)|a206>c,Q69>P(- - -)|g210>a|t213>c|g270>a|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)| a320>g| 319 (320) 221 (222) 206 15 (16) 3 (4) 12 1 (2) 5 2 1 3 0 1 0 0 1 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 51 51 46 5 0 5 1 1 1 0 1 0 1 0 0 0 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 0 1 0 0 117 114 107 7 3 4 0 3 1 1 2 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatacaccttcaccgtctactat ctattctgggtgcgacgggcccctggacaagggcttgagtggatgggatgg atcaaccctaagagtggtgacaca cactatgcaccgaaattccagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggaactgaataggctgagatctgacgacacggccgtgtattactgt gcgagaggccctgtcagtggctggtacactgactac ARGPVSGWYTDY IGHV1-2 IGHD6-19 IGHJ4 ggatacaccttcaccgtctactat ctattctgggtgcgacgggcccctggacaagggcttgagtggatgggatgg atcaaccctaagagtggtgacaca cactatgcaccgaaattccagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggaactgaataggctgagatctgacgacacggccgtgtattactgt gcgagaggccctgtcagtggctggtacactgactac IGA1 15 213 9 6 7 9 2 6 3 0 9 3 JY8QFUQ01A4U6K 10824 productive Homsap IGHV4-61*02 F 992 94.57 209/221 nt NA Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F 159 81.25 39/48 nt Homsap IGHD3-10*01 F 2 10 7 12 10.7.12 [1.17.38.11] CARGSGSGSYSNLW in-frame - tctctggtggctccatcagcagtggtagtcactactggagttggatccggcagcccgccgggaagggactggagtggattgggcgtatctatgccggttggaccaccaattacaatccctccctcaagagtcgagtcaccatatctatagacacgtcccagaaccagttctccctgaagctgagctctgtgaccgccgcggacacggccgtgtattactgtgcgagaggctcaggttcggggagttattctaacctctggggcccgggtaccctggtcaccgtctcctcagcctccaccaaggggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 94 [290] t109>c,Y37>H(+ - -)|c120>t|a172>g,T58>A(- - -)|a175>g,S59>G(+ + -)|g187>t,G63>W(- - -)|g191>c,S64>T(+ - +)|c198>t|c204>t|a237>t|g238>a,V80>I(+ - +)|a250>c,K84>Q(+ - -)|a291>g| t109>c,Y37>H(+ - -)| c120>t| a172>g,T58>A(- - -)|a175>g,S59>G(+ + -)|g187>t,G63>W(- - -)|g191>c,S64>T(+ - +)| c198>t|c204>t|a237>t|g238>a,V80>I(+ - +)|a250>c,K84>Q(+ - -)|a291>g| a320>g| 319 (320) 228 (229) 216 12 (13) 5 (6) 7 3 (4) 1 3 1 1 0 1 0 1 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 29 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 21 17 4 0 4 2 0 0 0 0 0 0 0 1 0 1 0 117 114 108 6 4 2 1 1 2 0 1 0 1 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcagtggtagtcactac tggagttggatccggcagcccgccgggaagggactggagtggattgggcgt atctatgccggttggaccacc aattacaatccctccctcaagagtcgagtcaccatatctatagacacgtcccagaaccagttctccctgaagctgagctctgtgaccgccgcggacacggccgtgtattactgt gcgagaggctcaggttcggggagttattctaacctc ARGSGSGSYSNL IGHV4-61 IGHD3-10 IGHJ4 ggtggctccatcagcagtggtagtcactac tggagttggatccggcagcccgccgggaagggactggagtggattgggcgt atctatgccggttggaccacc aattacaatccctccctcaagagtcgagtcaccatatctatagacacgtcccagaaccagttctccctgaagctgagctctgtgaccgccgcggacacggccgtgtattactgt gcgagaggctcaggttcggggagttattctaacctc IGG1 12 216 8 4 4 6 4 6 5 0 2 5 JY8QFUQ01A4UD9 10031 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 977 94.5 206/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-8*01 F 2 8 8 16 8.8.16 [1.17.38.11] CARDRGCGNGACHVADYW in-frame - cctctggattcaccttcagtaactatagtatgaactgggtccgccaggctccagggaagggcctggagtgggtctcatccatcagtagtggtggtagtttcaaacaccacgcagactcagcgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattattgtgcgagagatcggggttgtggtaatggtgcatgccatgtcgccgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [297] g107>a,S36>N(- - -)|c114>t|g147>c|t168>c|a175>g,S59>G(+ + -)|a184>g,S62>G(+ + -)|a191>t,Y64>F(- + -)|t194>a,I65>K(- + -)|t196>c,Y66>H(+ - -)|t199>c,Y67>H(+ - -)|t212>c,V71>A(+ - +)|c309>t| g107>a,S36>N(- - -)|c114>t| g147>c| t168>c|a175>g,S59>G(+ + -)|a184>g,S62>G(+ + -)|a191>t,Y64>F(- + -)|t194>a,I65>K(- + -)| t196>c,Y66>H(+ - -)|t199>c,Y67>H(+ - -)|t212>c,V71>A(+ - +)|c309>t| 320 226 214 12 4 8 2 1 2 4 0 0 1 1 1 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 19 5 1 4 2 0 0 1 0 0 1 1 0 0 0 0 117 114 110 4 1 3 0 0 1 3 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtaactatagt atgaactgggtccgccaggctccagggaagggcctggagtgggtctcatcc atcagtagtggtggtagtttcaaa caccacgcagactcagcgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattattgt gcgagagatcggggttgtggtaatggtgcatgccatgtcgccgactac ARDRGCGNGACHVADY IGHV3-21 IGHD2-8 IGHJ4 ggattcaccttcagtaactatagt atgaactgggtccgccaggctccagggaagggcctggagtgggtctcatcc atcagtagtggtggtagtttcaaa caccacgcagactcagcgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattattgt gcgagagatcggggttgtggtaatggtgcatgccatgtcgccgactac IGA2 12 213 9 3 3 4 6 8 2 2 3 5 JY8QFUQ01A4UI3 22246 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 938 91.86 203/221 nt 90.95 201/221 nt Homsap IGHJ6*02 F (see comment) 256 90.32 56/62 nt Homsap IGHD3-22*01 F 2 8 8 18 8.8.18 [2.17.38.11] CAHGPSAAPHYYYYYGLEAW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) in CDR1-IMGT at codon 28 of V-REGION: 'T' (1 nucleotides at position 14 to 14 in the user submitted sequence), (cause frameshift);in CDR1-IMGT at codon 38 of V-REGION: 'G' (1 nucleotides at position 32 to 32 in the user submitted sequence), (cause frameshift); aggcctctggattTcacctttaccaactatgGgcatgaggtgggtccgccaggctccagggaaggggctggagtgggtctcagatattagcgctggtggcgataacacatactacgcagactccgtgaagggccggttcaccatctccagagacaatttcaggaacacgctgtatctgcaaatgagcagcctgagagccgaggacacggccctttattactgtgcgcatgggcccagtgcagcaccccattactactactattacggtttggaagcttggggccaagggaccaaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [308] c71>a|a72>g|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)|c120>g,S40>R(- - -)|c164>a,A55>D(- - -)|t171>c|g173>c,G58>A(- + -)|a175>g,S59>G(+ + -)|t186>c|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|c248>t,S83>F(- - -)|a251>g,K84>R(+ + +)|a275>g,N92>S(- - -)|g301>c,V101>L(+ - +)|a303>t,V101>L(+ - +)| c71>a|a72>g| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)| c120>g,S40>R(- - -)|c164>a,A55>D(- - -)| t171>c|g173>c,G58>A(- + -)|a175>g,S59>G(+ + -)|t186>c|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)| c248>t,S83>F(- - -)|a251>g,K84>R(+ + +)|a275>g,N92>S(- - -)|g301>c,V101>L(+ - +)|a303>t,V101>L(+ - +)| a316>c,K106>H(- - +)|a318>t,K106>H(- - +)|a320>g| 315 (320) 224 (229) 206 (208) 18 (21) 4 (5) 14 (16) 4 (5) 3 1 2 0 (1) 2 1 (2) 0 3 2 0 0 78 8 6 2 2 0 1 0 0 0 0 1 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 49 2 0 2 0 0 0 0 0 1 0 0 0 1 0 0 30 24 18 6 2 4 1 2 0 2 0 0 0 0 1 0 0 0 117 114 109 5 0 5 2 0 1 0 0 0 1 0 1 0 0 0 3 (8) 3 3 (5) 0 (3) 0 (1) 0 (2) 0 (1) 0 0 0 0 (1) 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| aggcctct ggattcacctttaccaactatggc atgaggtgggtccgccaggctccagggaaggggctggagtgggtctcagat attagcgctggtggcgataacaca tactacgcagactccgtgaagggccggttcaccatctccagagacaatttcaggaacacgctgtatctgcaaatgagcagcctgagagccgaggacacggccctttattactgt gcgcatgggcccagtgcagcaccccattactactactattacggtttggaagct AHGPSAAPHYYYYYGLEA IGHV3-23 IGHD3-22 IGHJ6 ggattcacctttaccaactatggc atgaggtgggtccgccaggctccagggaaggggctggagtgggtctcagat attagcgctggtggcgataacaca tactacgcagactccgtgaagggccggttcaccatctccagagacaatttcaggaacacgctgtatctgcaaatgagcagcctgagagccgaggacacggccctttattactgt gcgcatgggcccagtgcagcaccccattactactactattacggtttggaagct IGA1 16 213 9 7 4 10 5 6 0 2 7 7 JY8QFUQ01A4VB0 22267 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 866 88.24 195/221 nt 87.78 194/221 nt Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 192 86.27 44/51 nt Homsap IGHD3-22*01 F 2 10 7 13 10.7.13 [1.17.38.11] CARQLSGDYASFDSW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 64 to 64 in the user submitted sequence), (cause frameshift); tctctggtgcctccatcaccagtggtactttttactgggcctggatccgccagcccccagggaAaggggctggagtgggttggcaatatctattctagtggtgtcgcctattacaacccgtccctcaagagtcgagtcaccatgtccgtcgacacgtccaagaatgagttttccctgacactgacctctgtgaccgccgcagacacggctgtatatttctgtgcgagacagctcagtggtgactacgcatcgtttgactcctggggccagggaaccctggtcgccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [292] g83>c,G28>A(- + -)|g92>c,S31>T(+ - +)|a103>g,S35>G(+ + -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|c111>t,Y37>F(- + -)|g119>c,G40>A(- + -)|a157>g,I53>V(+ - +)|g162>c|g164>a,S55>N(- - -)|a173>c,Y58>S(+ - -)|g189>t|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|a193>g,T65>A(- - -)|c198>t|a234>g,I78>M(+ + -)|a240>c|c255>t|c256>g,Q86>E(+ + -)|c261>t|a269>c,K90>T(- - -)|g270>a,K90>T(- - -)|g275>c,S92>T(+ - +)|g303>a|a308>t,Y103>F(- + -)| g83>c,G28>A(- + -)|g92>c,S31>T(+ - +)|a103>g,S35>G(+ + -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|c111>t,Y37>F(- + -)| g119>c,G40>A(- + -)|a157>g,I53>V(+ - +)|g162>c|g164>a,S55>N(- - -)| a173>c,Y58>S(+ - -)|g189>t|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|a193>g,T65>A(- - -)| c198>t|a234>g,I78>M(+ + -)|a240>c|c255>t|c256>g,Q86>E(+ + -)|c261>t|a269>c,K90>T(- - -)|g270>a,K90>T(- - -)|g275>c,S92>T(+ - +)|g303>a|a308>t,Y103>F(- + -)| 320 229 203 26 7 19 5 3 4 0 3 0 2 0 6 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 0 6 1 0 1 0 0 0 1 0 3 0 0 0 51 51 47 4 1 3 1 1 0 0 0 0 0 0 2 0 0 0 30 21 16 5 1 4 2 0 0 0 1 0 0 0 0 0 2 0 117 114 103 11 5 6 1 2 3 0 2 0 1 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgcctccatcaccagtggtactttttac tgggcctggatccgccagcccccagggaaggggctggagtgggttggcaat atctattctagtggtgtcgcc tattacaacccgtccctcaagagtcgagtcaccatgtccgtcgacacgtccaagaatgagttttccctgacactgacctctgtgaccgccgcagacacggctgtatatttctgt gcgagacagctcagtggtgactacgcatcgtttgactcc ARQLSGDYASFDS IGHV4-39 IGHD3-22 IGHJ5 ggtgcctccatcaccagtggtactttttac tgggcctggatccgccagcccccagggaaggggctggagtgggttggcaat atctattctagtggtgtcgcc tattacaacccgtccctcaagagtcgagtcaccatgtccgtcgacacgtccaagaatgagttttccctgacactgacctctgtgaccgccgcagacacggctgtatatttctgt gcgagacagctcagtggtgactacgcatcgtttgactcc IGA2 26 216 12 14 7 16 5 10 6 1 9 10 JY8QFUQ01A4W5D 6317 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 921 92.52 198/214 nt NA Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD2-21*01 F 2 8 7 15 8.7.15 [1.17.38.11] CTRELRLAAARDGVDIW in-frame + ctctgggttcaccgtcagtagcaagttcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaattacttatcctgatggtactacatattatggagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgtacgagagaacttaggctggctgctgcgcgtgatggtgttgacatctggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgcaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 71 95 [290, 292] c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)|g163>a,V55>I(+ - +)|t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|c198>t|c201>t|c203>g,A68>G(- + -)|t262>g,Y88>D(- - -)|g303>t|c309>t|g313>a,A105>T(- - -)| c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)| g163>a,V55>I(+ - +)| t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)| c198>t|c201>t|c203>g,A68>G(- + -)|t262>g,Y88>D(- - -)|g303>t|c309>t| g313>a,A105>T(- - -)| 320 222 205 17 4 13 0 3 5 1 1 0 1 0 2 2 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 21 14 7 0 7 0 1 2 1 1 0 0 0 2 0 0 0 117 114 108 6 4 2 0 0 3 0 0 0 0 0 0 1 1 1 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcaccgtcagtagcaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaatt acttatcctgatggtactaca tattatggagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttaggctggctgctgcgcgtgatggtgttgacatc TRELRLAAARDGVDI IGHV3-53 IGHD2-21 IGHJ3 gggttcaccgtcagtagcaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaatt acttatcctgatggtactaca tattatggagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttaggctggctgctgcgcgtgatggtgttgacatc IGA2 16 210 8 8 7 12 1 4 4 0 3 9 JY8QFUQ01A4X8T 8148 productive Homsap IGHV3-9*01 F 855 88.48 192/217 nt NA Homsap IGHJ6*02 F 238 87.1 54/62 nt Homsap IGHD4-17*01 F 2 8 8 12 8.8.12 [1.17.38.11] CARTYGDHDGMDVW in-frame + ctctggattcacctttgacgactatggcatgcactgggtccggcaaggtccagggaagggcctggagtgggtctcaagtatcagttggaacagtgctaagatagactatgcggacgctgtgaagggccgattcaccacatccagagacaacgccaagaactccatctttttgcaaatgaacagtctgagaaatgacgacacggccttctacttttgtgcaaggacttacggcgaccatgacggaatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] t105>c|t108>c|c113>g,A38>G(- + -)|c134>g,A45>G(- + -)|g163>a,G55>S(+ + -)|t168>c|t177>c|g188>c,G63>A(- + -)|g191>a,S64>K(- - -)|c192>g,S64>K(- - -)|g197>a,G66>D(- - -)|t208>g,S70>A(- + -)|t233>c,I78>T(- - -)|c234>a,I78>T(- - -)|c259>a,L87>I(+ + +)|g261>c,L87>I(+ + +)|a263>t,Y88>F(- + -)|c265>t|g286>a,A96>N(- - -)|c287>a,A96>N(- - -)|g291>c,E97>D(+ - +)|g303>c,L101>F(+ - -)|t306>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| t105>c|t108>c|c113>g,A38>G(- + -)| c134>g,A45>G(- + -)|g163>a,G55>S(+ + -)| t168>c|t177>c|g188>c,G63>A(- + -)|g191>a,S64>K(- - -)|c192>g,S64>K(- - -)| g197>a,G66>D(- - -)|t208>g,S70>A(- + -)|t233>c,I78>T(- - -)|c234>a,I78>T(- - -)|c259>a,L87>I(+ + +)|g261>c,L87>I(+ + +)|a263>t,Y88>F(- + -)|c265>t|g286>a,A96>N(- - -)|c287>a,A96>N(- - -)|g291>c,E97>D(+ - +)|g303>c,L101>F(+ - -)|t306>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| a317>g,K106>R(+ + +)|a318>g,K106>R(+ + +)|g319>a,D107>T(- + -)|a320>c,D107>T(- + -)|a322>t| 316 (322) 221 (227) 196 (197) 25 (30) 6 (7) 19 (23) 0 (2) 4 (5) 2 6 0 (1) 3 2 (3) 0 4 3 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 0 2 0 0 0 0 0 1 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 0 1 0 0 30 24 19 5 2 3 0 1 0 2 0 0 0 0 1 1 0 0 117 114 99 15 2 13 0 2 2 2 0 3 2 0 3 0 0 1 4 (10) 4 4 (5) 0 (5) 0 (1) 0 (4) 0 (2) 0 (1) 0 0 0 (1) 0 0 (1) 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttgacgactatggc atgcactgggtccggcaaggtccagggaagggcctggagtgggtctcaagt atcagttggaacagtgctaagata gactatgcggacgctgtgaagggccgattcaccacatccagagacaacgccaagaactccatctttttgcaaatgaacagtctgagaaatgacgacacggccttctacttttgt gcaaggacttacggcgaccatgacggaatggacgtc ARTYGDHDGMDV IGHV3-9 IGHD4-17 IGHJ6 ggattcacctttgacgactatggc atgcactgggtccggcaaggtccagggaagggcctggagtgggtctcaagt atcagttggaacagtgctaagata gactatgcggacgctgtgaagggccgattcaccacatccagagacaacgccaagaactccatctttttgcaaatgaacagtctgagaaatgacgacacggccttctacttttgt gcaaggacttacggcgaccatgacggaatggacgtc IGA1 25 213 12 13 6 16 6 9 2 4 15 4 JY8QFUQ01A4XPO 6897 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 855 88.48 192/217 nt NA Homsap IGHJ6*02 F 229 85.48 53/62 nt Homsap IGHD5-12*01 F 2 8 8 19 8.8.19 [1.17.38.11] CARDGSAHSSIYHYYYGMDVW in-frame + ctctggattcaccttcagttctcacactatgaactgggtccggcaggctccagggaaggggccggagtgggtctcaaccattggtactagaggtagatccatatactacgcggactcagtgaagggccgattcaccacctccagagacaacgccaagaaatcactttatctggatatgcacagcctgagagccgaggacacggctatctattactgtgcgagagatggcagtgcacactcgtcgatctatcattactattacggcatggacgtctggggccaagggaccacggtcatcatctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcacgcacccagccagatgggaacgtcgtcatcgcctgcctggtccagggcttcttcc NA IGA1 79 79 [305, 306] a106>t|g107>c|c108>t|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)|g113>c,S38>T(+ - +)|c114>t,S38>T(+ - +)|c129>g|t149>c,L50>P(- - -)|t163>a,S55>T(+ - +)|a169>g,S57>G(+ + -)|g173>c,S58>T(+ - +)|t177>a,S59>R(- - -)|a184>g,S62>G(+ + -)|t189>a,S63>R(- - -)|a191>c,Y64>S(+ - -)|a204>g|t233>c,I78>T(- - -)|c255>a,N85>K(+ - -)|g261>t|c268>g,Q90>D(+ - -)|a270>t,Q90>D(+ - -)|a274>c,N92>H(- - -)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)| a106>t|g107>c|c108>t|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)|g113>c,S38>T(+ - +)|c114>t,S38>T(+ - +)| c129>g|t149>c,L50>P(- - -)|t163>a,S55>T(+ - +)| a169>g,S57>G(+ + -)|g173>c,S58>T(+ - +)|t177>a,S59>R(- - -)|a184>g,S62>G(+ + -)|t189>a,S63>R(- - -)|a191>c,Y64>S(+ - -)| a204>g|t233>c,I78>T(- - -)|c255>a,N85>K(+ - -)|g261>t|c268>g,Q90>D(+ - -)|a270>t,Q90>D(+ - -)|a274>c,N92>H(- - -)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)| 320 225 200 25 6 19 3 1 2 4 2 1 2 3 4 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 3 4 0 0 2 2 0 0 1 0 2 0 0 0 51 51 48 3 1 2 0 0 0 1 0 0 0 1 0 1 0 0 30 24 18 6 0 6 2 0 0 0 1 0 0 2 1 0 0 0 117 114 105 9 2 7 1 1 0 1 1 1 1 0 1 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagttctcacact atgaactgggtccggcaggctccagggaaggggccggagtgggtctcaacc attggtactagaggtagatccata tactacgcggactcagtgaagggccgattcaccacctccagagacaacgccaagaaatcactttatctggatatgcacagcctgagagccgaggacacggctatctattactgt gcgagagatggcagtgcacactcgtcgatctatcattactattacggcatggacgtc ARDGSAHSSIYHYYYGMDV IGHV3-21 IGHD5-12 IGHJ6 ggattcaccttcagttctcacact atgaactgggtccggcaggctccagggaaggggccggagtgggtctcaacc attggtactagaggtagatccata tactacgcggactcagtgaagggccgattcaccacctccagagacaacgccaagaaatcactttatctggatatgcacagcctgagagccgaggacacggctatctattactgt gcgagagatggcagtgcacactcgtcgatctatcattactattacggcatggacgtc IGA1 25 213 10 15 3 11 7 14 3 3 9 10 JY8QFUQ01A4XSE 12416 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 958 93.98 203/216 nt NA Homsap IGHJ4*02 F (see comment) 177 85.42 41/48 nt Homsap IGHD5-12*01 F 1 8 8 9 8.8.9 [1.17.38.11] CARLPNTIDSW in-frame - Other possibilities: Homsap_IGHJ5*01 (highest number of consecutive identical nucleotides) tctggatatagttttgccacctactggatcggctgggtgcgccagaggcccgggaagggcctggagtggatgggggtcatctatcctggtgactctgataccagatacagcccgtccttccaaggccgggtcaccatttcagccgacaagtccctcagtatcgcctacctgcagtggagcagcctgaaggcctcggacaccgccatatattactgtgcgagactgcctaacactattgactcctggggccaggggaccctggtcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 100 [274] c84>t|c87>t|a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)|t134>g,M45>R(- + -)|a144>g|a163>g,I55>V(+ - +)|a224>g,Q75>R(+ - -)|c234>t|a250>c,I84>L(+ + +)|c255>t|c257>t,T86>I(- - -)|g303>a,M101>I(+ + -)| c84>t|c87>t|a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)| t134>g,M45>R(- + -)|a144>g|a163>g,I55>V(+ - +)| a224>g,Q75>R(+ - -)|c234>t|a250>c,I84>L(+ + +)|c255>t|c257>t,T86>I(- - -)|g303>a,M101>I(+ + -)| a320>t| 319 (320) 223 (224) 210 13 (14) 5 (6) 8 4 1 5 0 1 0 0 (1) 0 1 0 0 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 1 0 2 0 0 0 0 0 1 0 0 0 51 51 48 3 1 2 2 0 0 0 0 0 0 0 0 0 0 1 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 2 4 1 1 3 0 1 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| tct ggatatagttttgccacctactgg atcggctgggtgcgccagaggcccgggaagggcctggagtggatgggggtc atctatcctggtgactctgatacc agatacagcccgtccttccaaggccgggtcaccatttcagccgacaagtccctcagtatcgcctacctgcagtggagcagcctgaaggcctcggacaccgccatatattactgt gcgagactgcctaacactattgactcc ARLPNTIDS IGHV5-51 IGHD5-12 IGHJ4 ggatatagttttgccacctactgg atcggctgggtgcgccagaggcccgggaagggcctggagtggatgggggtc atctatcctggtgactctgatacc agatacagcccgtccttccaaggccgggtcaccatttcagccgacaagtccctcagtatcgcctacctgcagtggagcagcctgaaggcctcggacaccgccatatattactgt gcgagactgcctaacactattgactcc IGA1 13 213 10 3 6 7 4 6 3 2 6 2 JY8QFUQ01A528Y 4140 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 918 91.71 199/217 nt NA Homsap IGHJ5*02 F 255 100 51/51 nt Homsap IGHD3-22*01 F 1 8 8 16 8.8.16 [1.17.38.11] CAKAGRGLIRPNNWFDPW in-frame - ctctggatttaccttcagtggctatggcatgcactgggtccgccaggctccaggcaagggcctggagtgggtgacagttgtttcatatgatggaagtattaagaattatgcagactccgtgaagggccgattcaccatctccagagacgattccaagaatacgctgtatctgcaaatgagcagcctgggacctgaagacacggctatatattactgtgcgaaagcggggcgtgggttgatacgacccaacaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [296, 295] c84>t|a106>g,S36>G(+ + -)|g147>c|g160>a,A54>T(- - -)|a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|a191>t,N64>I(- - -)|a195>g|t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|a244>g,N82>D(+ + -)|c255>t|a275>g,N92>S(- - -)|a283>g,R95>G(- - -)|g286>c,A96>P(- - -)|g291>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|g317>a,R106>K(+ + +)| c84>t|a106>g,S36>G(+ + -)| g147>c|g160>a,A54>T(- - -)| a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|a191>t,N64>I(- - -)|a195>g| t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|a244>g,N82>D(+ + -)|c255>t|a275>g,N92>S(- - -)|a283>g,R95>G(- - -)|g286>c,A96>P(- - -)|g291>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g317>a,R106>K(+ + +)|a320>c| 319 (320) 224 (225) 205 19 (20) 5 (6) 14 6 5 3 0 0 (1) 0 2 1 2 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 1 0 1 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 30 24 20 4 1 3 2 0 0 0 0 0 2 0 0 0 0 0 117 114 104 10 2 8 3 3 2 0 0 0 0 1 1 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 1 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggatttaccttcagtggctatggc atgcactgggtccgccaggctccaggcaagggcctggagtgggtgacagtt gtttcatatgatggaagtattaag aattatgcagactccgtgaagggccgattcaccatctccagagacgattccaagaatacgctgtatctgcaaatgagcagcctgggacctgaagacacggctatatattactgt gcgaaagcggggcgtgggttgatacgacccaacaactggttcgacccc AKAGRGLIRPNNWFDP IGHV3-30 IGHD3-22 IGHJ5 ggatttaccttcagtggctatggc atgcactgggtccgccaggctccaggcaagggcctggagtgggtgacagtt gtttcatatgatggaagtattaag aattatgcagactccgtgaagggccgattcaccatctccagagacgattccaagaatacgctgtatctgcaaatgagcagcctgggacctgaagacacggctatatattactgt gcgaaagcggggcgtgggttgatacgacccaacaactggttcgacccc IGA2 18 213 13 5 7 9 6 9 3 2 9 4 JY8QFUQ01A53DH 20215 productive (see comment) Homsap IGHV3-48*01 F (see comment) 774 84.33 183/217 nt 83.87 182/217 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD5-12*01 F 3 8 7 12 8.7.12 [2.17.38.11] CSRDGRRGYDLDSW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (84,33% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR2-IMGT, from codon 57 of V-REGION: 3 nucleotides (from position 86 in the user submitted sequence), (do not cause frameshift) agcctctggattcaccttcggaacctatgccatgacgtgggtccgcctgactcctgggaaagggctggagtgggtttcatggattagtgatatcggtgacacacgctatgcagattctgtgaagggccgattcaccatctccagagacaatgccaagaattcactgtttctgcaaatggacagtctcagagccgacgacacggctatatattattgttcgagagatggccggaggggctacgaccttgactcctggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [284] a103>g,S35>G(+ + -)|t105>a,S35>G(+ + -)|g107>c,S36>T(+ - +)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)|a119>c,N40>T(- + -)|c120>g,N40>T(- + -)|a131>t,Q44>L(- - -)|g133>a,A45>T(- - -)|a138>t|g144>a|a164>g,Y55>W(- + -)|c165>g,Y55>W(- + -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g185>t,S62>I(- - -)|t186>c,S62>I(- - -)|a187>g,S63>G(+ + -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|t194>c,I65>T(- - -)|t196>c,Y66>R(- - -)|a197>g,Y66>R(- - -)|c201>t|c207>t|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|g282>c|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t|g313>t,A105>S(- + -)| a103>g,S35>G(+ + -)|t105>a,S35>G(+ + -)|g107>c,S36>T(+ - +)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)| a119>c,N40>T(- + -)|c120>g,N40>T(- + -)|a131>t,Q44>L(- - -)|g133>a,A45>T(- - -)|a138>t|g144>a|a164>g,Y55>W(- + -)|c165>g,Y55>W(- + -)| a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g185>t,S62>I(- - -)|t186>c,S62>I(- - -)|a187>g,S63>G(+ + -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|t194>c,I65>T(- - -)| t196>c,Y66>R(- - -)|a197>g,Y66>R(- - -)|c201>t|c207>t|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|g282>c|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| g313>t,A105>S(- + -)| 320 225 190 35 8 27 8 5 5 3 1 1 3 1 4 2 2 0 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 2 0 0 0 0 0 0 1 2 0 0 0 51 51 43 8 2 6 1 2 0 0 1 0 2 0 0 2 0 0 30 21 13 8 0 8 3 1 0 2 0 1 0 0 0 0 1 0 117 114 101 13 6 7 2 2 5 1 0 0 1 0 2 0 0 0 8 8 7 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct ggattcaccttcggaacctatgcc atgacgtgggtccgcctgactcctgggaaagggctggagtgggtttcatgg attagtgatatcggtgacaca cgctatgcagattctgtgaagggccgattcaccatctccagagacaatgccaagaattcactgtttctgcaaatggacagtctcagagccgacgacacggctatatattattgt tcgagagatggccggaggggctacgaccttgactcc SRDGRRGYDLDS IGHV3-48 IGHD5-12 IGHJ4 ggattcaccttcggaacctatgcc atgacgtgggtccgcctgactcctgggaaagggctggagtgggtttcatgg attagtgatatcggtgacaca cgctatgcagattctgtgaagggccgattcaccatctccagagacaatgccaagaattcactgtttctgcaaatggacagtctcagagccgacgacacggctatatattattgt tcgagagatggccggaggggctacgaccttgactcc IGA1 34 210 21 13 10 18 11 16 8 0 13 13 JY8QFUQ01A53VQ 4916 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 927 92.17 200/217 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD6-19*01 F 1 8 8 13 8.8.13 [1.17.38.11] CAKGYSSGWYDFDCW in-frame + ctctggattcacctttagtagttttgccatgacctgggtccgtcaggctccagggaaggggctggagtgggtctcaactattcattataatggtgataacacatactacgcagactccgtgaggggccgattcaccatctccagagacgattccaagaccacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaaggggtatagcagtggctggtacgactttgactgctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG2 100 100 [288] c105>t|c108>t|a110>t,Y37>F(- + -)|g119>c,S40>T(+ - +)|c129>t|g163>a,A55>T(- - -)|a169>c,S57>H(+ - -)|g170>a,S57>H(+ - -)|g172>t,G58>Y(+ - -)|g173>a,G58>Y(+ - -)|g176>a,S59>N(- - -)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|a215>g,K72>R(+ + +)|g225>a|a244>g,N82>D(+ + -)|a254>c,N85>T(- + -)| c105>t|c108>t|a110>t,Y37>F(- + -)| g119>c,S40>T(+ - +)|c129>t|g163>a,A55>T(- - -)| a169>c,S57>H(+ - -)|g170>a,S57>H(+ - -)|g172>t,G58>Y(+ - -)|g173>a,G58>Y(+ - -)|g176>a,S59>N(- - -)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)| a215>g,K72>R(+ + +)|g225>a|a244>g,N82>D(+ + -)|a254>c,N85>T(- + -)| a318>g|a320>g| 317 (320) 222 (225) 205 (206) 17 (19) 4 (6) 13 2 (4) 7 3 0 2 0 1 0 1 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 2 0 0 0 1 0 0 0 0 0 51 51 48 3 1 2 0 1 1 0 0 0 0 0 1 0 0 0 30 24 17 7 0 7 0 5 0 0 1 0 0 0 0 0 1 0 117 114 110 4 1 3 2 1 0 0 1 0 0 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtagttttgcc atgacctgggtccgtcaggctccagggaaggggctggagtgggtctcaact attcattataatggtgataacaca tactacgcagactccgtgaggggccgattcaccatctccagagacgattccaagaccacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaggggtatagcagtggctggtacgactttgactgc AKGYSSGWYDFDC IGHV3-23 IGHD6-19 IGHJ4 ggattcacctttagtagttttgcc atgacctgggtccgtcaggctccagggaaggggctggagtgggtctcaact attcattataatggtgataacaca tactacgcagactccgtgaggggccgattcaccatctccagagacgattccaagaccacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaggggtatagcagtggctggtacgactttgactgc IGG2 17 213 12 5 10 12 2 5 2 2 5 8 JY8QFUQ01A554Z 19173 productive (see comment) Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 873 89.4 194/217 nt 88.02 191/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARKRVPGGTFYGDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'G' (1 nucleotides at position 20 to 20 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 48 of V-REGION: 'G' (1 nucleotides at position 59 to 59 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 65 of V-REGION: 'C' (1 nucleotides at position 103 to 103 in the user submitted sequence), (cause frameshift); ctctggattcacctttagtGacctatggcatgctctgggtccgccatgttgcaggcaaGggggctggagtgggtggcaactatatcagctgatggacgaaatCaaatactatgcagattccgtgatgggccgattcgccctctccagagacaaatccaagaacacggattatctgcaaatgaacagcctgagaactgacgacacggctgtatattactgtgcgagaaagagagtccccggggggactttttatggggactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [295, 294] c90>t|g107>c,S36>T(+ - +)|a119>t,H40>L(- - -)|g132>t,Q44>H(- + -)|c134>t,A45>V(+ - +)|c136>g,P46>A(- - -)|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)|t172>g,Y58>A(- - -)|a173>c,Y58>A(- - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)|c207>t|a215>t,K72>M(- + -)|a229>g,T77>A(- - -)|a232>c,I78>L(+ + +)|t246>a,N82>K(+ - -)|c259>g,L87>D(- - -)|t260>a,L87>D(- - -)|g261>t,L87>D(- - -)|g286>a,A96>T(- - -)|g291>c,E97>D(+ - +)|g303>a| c90>t|g107>c,S36>T(+ - +)| a119>t,H40>L(- - -)|g132>t,Q44>H(- + -)|c134>t,A45>V(+ - +)|c136>g,P46>A(- - -)|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)| t172>g,Y58>A(- - -)|a173>c,Y58>A(- - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)| c207>t|a215>t,K72>M(- + -)|a229>g,T77>A(- - -)|a232>c,I78>L(+ + +)|t246>a,N82>K(+ - -)|c259>g,L87>D(- - -)|t260>a,L87>D(- - -)|g261>t,L87>D(- - -)|g286>a,A96>T(- - -)|g291>c,E97>D(+ - +)|g303>a| g319>a| 318 (320) 223 (225) 200 (201) 23 (24) 3 (4) 20 1 3 (4) 3 1 3 0 2 3 2 2 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 1 0 0 0 0 0 1 0 0 0 51 51 45 6 0 6 0 1 1 1 0 0 1 0 0 1 1 0 30 24 20 4 0 4 0 0 0 0 2 0 0 1 0 0 0 1 117 114 103 11 2 9 1 2 1 0 1 0 1 2 1 1 1 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtacctatggc atgctctgggtccgccatgttgcaggcaaggggctggagtgggtggcaact atatcagctgatggacgaaataaa tactatgcagattccgtgatgggccgattcgccctctccagagacaaatccaagaacacggattatctgcaaatgaacagcctgagaactgacgacacggctgtatattactgt gcgagaaagagagtccccggggggactttttatggggactac ARKRVPGGTFYGDY IGHV3-30 IGHD3-10 IGHJ4 ggattcacctttagtacctatggc atgctctgggtccgccatgttgcaggcaaggggctggagtgggtggcaact atatcagctgatggacgaaataaa tactatgcagattccgtgatgggccgattcgccctctccagagacaaatccaagaacacggattatctgcaaatgaacagcctgagaactgacgacacggctgtatattactgt gcgagaaagagagtccccggggggactttttatggggactac IGG2 23 213 8 15 6 12 2 11 2 1 15 5 JY8QFUQ01A55FP 17930 productive Homsap IGHV3-7*01 F 1014 95.91 211/220 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 11 8.8.11 [2.17.38.11] CAISSGYYSTGYW in-frame - aggctctggattcagctttagttactattggatgagctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaaggaagatggaagtgagagacactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgtgcgattagtagtggttattactcgacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccag NA IGA2 100 100 [284] c74>g,A25>G(- + -)|c86>g,T29>S(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)|t196>c,Y66>H(+ - -)|a254>g,N85>S(- - -)|g303>a| c74>g,A25>G(- + -)| c86>g,T29>S(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)| c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)| t196>c,Y66>H(+ - -)|a254>g,N85>S(- - -)|g303>a| g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 316 (320) 224 (228) 215 9 (13) 1 (3) 8 (10) 2 (3) 2 (3) 0 1 0 0 1 (2) 0 0 3 0 (1) 0 78 7 6 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 36 24 21 3 0 3 0 1 0 0 0 0 1 0 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 1 0 0 117 114 111 3 1 2 1 1 0 1 0 0 0 0 0 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| aggctct ggattcagctttagttactattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgattagtagtggttattactcgacgggctac AISSGYYSTGY IGHV3-7 IGHD3-22 IGHJ4 ggattcagctttagttactattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgattagtagtggttattactcgacgggctac IGA2 8 213 5 3 2 4 3 4 1 0 2 5 JY8QFUQ01A56UP 18052 productive Homsap IGHV4-61*02 F 897 90 198/220 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-10*02 F 3 10 7 16 10.7.16 [1.17.38.11] CAREHSRTMGGARELDSW in-frame + ctctggtggccccatcagcagtggtacttactactggacttggatccggcaggccgccggggagggactggagtggatcggacgtgtttatattagtgggagcaccacctacaatccctccctcaagagtcgaatcaccatatcactagacacgtccaagaaccagatcttcctgaagttgaggtctgtgaccgccgctgacacggccgtatattactgtgcgagagaacatagtaggacgatgggcggggcgagagagcttgactcctggggcctgggaactctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [299] t85>c,S29>P(+ - -)|g107>c,S36>T(+ - +)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|c133>g,P45>A(- - -)|a142>g,K48>E(+ - -)|t159>c|g162>a|a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|c173>t,T58>I(- - -)|c174>t,T58>I(- - -)|a197>c,N66>T(- + -)|c204>t|g226>a,V76>I(+ - +)|g238>c,V80>L(+ - +)|t259>a,F87>I(+ - -)|c263>t,S88>F(- - -)|c271>t|c276>g,S92>R(- - -)|a291>t|g303>a| t85>c,S29>P(+ - -)|g107>c,S36>T(+ - +)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|c133>g,P45>A(- - -)|a142>g,K48>E(+ - -)|t159>c|g162>a| a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|c173>t,T58>I(- - -)|c174>t,T58>I(- - -)| a197>c,N66>T(- + -)|c204>t|g226>a,V76>I(+ - +)|g238>c,V80>L(+ - +)|t259>a,F87>I(+ - -)|c263>t,S88>F(- - -)|c271>t|c276>g,S92>R(- - -)|a291>t|g303>a| 320 228 206 22 6 16 2 3 7 2 1 0 1 1 3 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 0 2 0 0 0 1 0 0 0 0 1 0 0 0 51 51 45 6 2 4 1 1 1 1 0 0 0 0 1 1 0 0 30 21 17 4 0 4 1 0 3 0 0 0 0 0 0 0 0 0 117 114 104 10 4 6 0 2 3 0 1 0 1 1 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtggccccatcagcagtggtacttactac tggacttggatccggcaggccgccggggagggactggagtggatcggacgt gtttatattagtgggagcacc acctacaatccctccctcaagagtcgaatcaccatatcactagacacgtccaagaaccagatcttcctgaagttgaggtctgtgaccgccgctgacacggccgtatattactgt gcgagagaacatagtaggacgatgggcggggcgagagagcttgactcc AREHSRTMGGARELDS IGHV4-61 IGHD3-10 IGHJ4 ggtggccccatcagcagtggtacttactac tggacttggatccggcaggccgccggggagggactggagtggatcggacgt gtttatattagtgggagcacc acctacaatccctccctcaagagtcgaatcaccatatcactagacacgtccaagaaccagatcttcctgaagttgaggtctgtgaccgccgctgacacggccgtatattactgt gcgagagaacatagtaggacgatgggcggggcgagagagcttgactcc IGA1 22 216 14 8 10 15 4 7 6 0 10 6 JY8QFUQ01A572P 16250 productive (see comment) Homsap IGHV3-30*01 F 981 94.93 206/217 nt 94.47 205/217 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-22*01 F 2 8 8 18 8.8.18 [1.17.38.11] CAGDLTGVPYYYDSSGLKYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 12 to 12 in the user submitted sequence), (cause frameshift); ctctagattcaGccttcagtagctataatatgcactgggtccgccaggctccaggcaaggggctagagtgggtggcagtcatattatatgatggaagtaacagttattacgcagactccgtgaagggccgattcaccgtctccagagacaattccaagaacacactgtatctgcaaatgaacagcctgagagctgaggacacggctgtgtattactgtgcgggagatttaactggggttccgtattattatgacagtagtggtttaaagtactggggccagggaaccctggtcaccgtctcctcagcatccccgaccaaccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [303] g79>a,G27>R(- - -)|g112>a,A38>N(- - -)|c113>a,A38>N(- - -)|t165>c|c170>t,S57>L(- - -)|t192>c|a194>g,K65>S(- - -)|a195>t,K65>S(- - -)|c198>t|a232>g,I78>V(+ - +)|g258>a|a316>g,R106>G(- - -)| g79>a,G27>R(- - -)|g112>a,A38>N(- - -)|c113>a,A38>N(- - -)| t165>c| c170>t,S57>L(- - -)|t192>c|a194>g,K65>S(- - -)|a195>t,K65>S(- - -)| c198>t|a232>g,I78>V(+ - +)|g258>a| a316>g,R106>G(- - -)| 320 225 213 12 4 8 3 3 2 2 0 1 1 0 0 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 2 0 0 0 1 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 30 24 20 4 1 3 1 0 1 1 0 0 1 0 0 0 0 0 117 114 111 3 2 1 1 1 1 0 0 0 0 0 0 0 0 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct agattcaccttcagtagctataat atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcagtc atattatatgatggaagtaacagt tattacgcagactccgtgaagggccgattcaccgtctccagagacaattccaagaacacactgtatctgcaaatgaacagcctgagagctgaggacacggctgtgtattactgt gcgggagatttaactggggttccgtattattatgacagtagtggtttaaagtac AGDLTGVPYYYDSSGLKY IGHV3-30 IGHD3-22 IGHJ4 agattcaccttcagtagctataat atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcagtc atattatatgatggaagtaacagt tattacgcagactccgtgaagggccgattcaccgtctccagagacaattccaagaacacactgtatctgcaaatgaacagcctgagagctgaggacacggctgtgtattactgt gcgggagatttaactggggttccgtattattatgacagtagtggtttaaagtac IGA1 11 213 9 2 5 6 4 5 3 1 1 6 JY8QFUQ01A58UD 14008 productive (see comment) Homsap IGHV3-7*02 F 927 92.17 200/217 nt 91.71 199/217 nt Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD5-12*01 F 1 7 8 9 7.8.9 [1.17.38.11] CARDQVTEDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 29 of V-REGION: 1 nucleotides (from position 12 in the user submitted sequence), (cause frameshift) cctctggattccttttagaacctattggatgagttgggtccgccaggctccagggaaagggctggagtgggtggcccacataaaccaagatggacgtgaggcatactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgtgccagagatcaagtgactgaagactactggggccagggaaccctggtcagtgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [276] c87>t|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|c120>t|a163>c,N55>H(- - -)|g171>c,K57>N(+ - -)|a187>c,S63>R(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)|c248>g,A83>G(- + -)|c255>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|g297>c|g301>a,V101>M(+ - -)|c309>t|g315>c| c87>t|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)| c120>t|a163>c,N55>H(- - -)| g171>c,K57>N(+ - -)|a187>c,S63>R(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)| c248>g,A83>G(- + -)|c255>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|g297>c|g301>a,V101>M(+ - -)|c309>t| g315>c| 318 223 205 18 7 11 1 2 5 0 3 0 0 1 4 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 20 3 1 2 0 0 1 0 0 0 0 1 1 0 0 0 51 51 49 2 1 1 0 0 1 0 1 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 0 0 2 0 0 0 1 0 0 0 117 114 106 8 4 4 0 2 3 0 0 0 0 0 1 2 0 0 6 6 5 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattccttttagaacctattgg atgagttgggtccgccaggctccagggaaagggctggagtgggtggcccac ataaaccaagatggacgtgaggca tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac ARDQVTEDY IGHV3-7 IGHD5-12 IGHJ4 ggattccttttagaacctattgg atgagttgggtccgccaggctccagggaaagggctggagtgggtggcccac ataaaccaagatggacgtgaggca tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac IGG1 17 212 8 9 7 12 1 5 5 1 5 6 JY8QFUQ01A5935 5957 productive Homsap IGHV4-61*02 F 875 88.69 196/221 nt NA Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F 150 79.17 38/48 nt Homsap IGHD3-10*02 F 3 10 7 16 10.7.16 [1.17.38.11] CAREHSRTMGGARELDSW in-frame + tctctggtggccccatcagcagcagcacttactactggacttggatccggcaggccgccggggagggactggagtggatcggacgtgtttatattagtgggagcaccaactacaatccctccctcaagagtcgaatcaccatatcactagacacgtccaagaaccagatcttcctgaagttgaggtctgtgaccgcctctgacacggccgtatattactgtgcgagagaacatagcaggacgatgggcggggcgagagagctagactcctggggcccgggcactctggtcaccgtctcctcagcatcccgacagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaaccgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 74 [298, 299] t85>c,S29>P(+ - -)|t102>c|g103>a,G35>S(+ + -)|t105>c,G35>S(+ + -)|g107>c,S36>T(+ - +)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|c133>g,P45>A(- - -)|a142>g,K48>E(+ - -)|t159>c|g162>a|a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|c173>t,T58>I(- - -)|c174>t,T58>I(- - -)|c204>t|g226>a,V76>I(+ - +)|g238>c,V80>L(+ - +)|t259>a,F87>I(+ - -)|c263>t,S88>F(- - -)|c271>t|c276>g,S92>R(- - -)|g289>t,A97>S(- + -)|a291>t,A97>S(- + -)|g303>a| t85>c,S29>P(+ - -)|t102>c|g103>a,G35>S(+ + -)|t105>c,G35>S(+ + -)|g107>c,S36>T(+ - +)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|c133>g,P45>A(- - -)|a142>g,K48>E(+ - -)|t159>c|g162>a| a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|c173>t,T58>I(- - -)|c174>t,T58>I(- - -)| c204>t|g226>a,V76>I(+ - +)|g238>c,V80>L(+ - +)|t259>a,F87>I(+ - -)|c263>t,S88>F(- - -)|c271>t|c276>g,S92>R(- - -)|g289>t,A97>S(- + -)|a291>t,A97>S(- + -)|g303>a| 320 229 204 25 6 19 2 4 7 4 0 0 1 1 3 2 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 25 5 1 4 0 1 0 3 0 0 0 0 1 0 0 0 51 51 45 6 2 4 1 1 1 1 0 0 0 0 1 1 0 0 30 21 17 4 0 4 1 0 3 0 0 0 0 0 0 0 0 0 117 114 104 10 3 7 0 2 3 0 0 0 1 1 1 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggccccatcagcagcagcacttactac tggacttggatccggcaggccgccggggagggactggagtggatcggacgt gtttatattagtgggagcacc aactacaatccctccctcaagagtcgaatcaccatatcactagacacgtccaagaaccagatcttcctgaagttgaggtctgtgaccgcctctgacacggccgtatattactgt gcgagagaacatagcaggacgatgggcggggcgagagagctagactcc AREHSRTMGGARELDS IGHV4-61 IGHD3-10 IGHJ4 ggtggccccatcagcagcagcacttactac tggacttggatccggcaggccgccggggagggactggagtggatcggacgt gtttatattagtgggagcacc aactacaatccctccctcaagagtcgaatcaccatatcactagacacgtccaagaaccagatcttcctgaagttgaggtctgtgaccgcctctgacacggccgtatattactgt gcgagagaacatagcaggacgatgggcggggcgagagagctagactcc IGA1 25 216 17 8 11 17 6 8 5 1 11 8 JY8QFUQ01A5AND 5040 productive Homsap IGHV3-30*15 F 891 90.32 196/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD5-18*01 F 1 8 8 16 8.8.16 [1.17.38.11] CARNELPWDTREYYFDYW in-frame + ctccggattcacctttagtaaatattccatgaactgggtccgccaggctccaggcaaggggctcgagtgggtggcacttatatcatacgatggaagtagaaaaatctacgcagattccgtgaagggccgattcaccatctccagagacaattccaagaacacggtatatctccaaatgagtagcctgagacctgaggacacggctgtctattactgtgcgaggaatgagctgccttgggatacaagagaatactactttgactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagcccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [295, 296] t78>c|c90>t|g107>a,S36>K(- - -)|c108>a,S36>K(- - -)|g112>t,A38>S(- + -)|t114>c,A38>S(- + -)|c118>a,H40>N(- - -)|a150>c|g163>c,V55>L(+ - +)|t174>c|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)|t196>a,Y66>I(- - -)|a197>t,Y66>I(- - -)|c207>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|g267>c|c276>t|g286>c,A96>P(- - -)|g303>c| t78>c| c90>t|g107>a,S36>K(- - -)|c108>a,S36>K(- - -)|g112>t,A38>S(- + -)|t114>c,A38>S(- + -)| c118>a,H40>N(- - -)|a150>c|g163>c,V55>L(+ - +)| t174>c|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)| t196>a,Y66>I(- - -)|a197>t,Y66>I(- - -)|c207>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|g267>c|c276>t|g286>c,A96>P(- - -)|g303>c| a318>g|g319>a| 317 (320) 222 (225) 201 (202) 21 (23) 8 (10) 13 1 (2) 2 (3) 3 3 1 2 1 2 4 1 1 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 1 1 0 1 0 0 0 0 1 0 51 51 48 3 1 2 0 0 0 0 1 1 0 0 1 0 0 0 30 24 21 3 1 2 1 0 0 1 0 0 0 1 0 0 0 0 117 114 105 9 4 5 0 1 2 0 0 0 1 1 3 1 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agca,274-277(FR3)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcacctttagtaaatattcc atgaactgggtccgccaggctccaggcaaggggctcgagtgggtggcactt atatcatacgatggaagtagaaaa atctacgcagattccgtgaagggccgattcaccatctccagagacaattccaagaacacggtatatctccaaatgagtagcctgagacctgaggacacggctgtctattactgt gcgaggaatgagctgccttgggatacaagagaatactactttgactat ARNELPWDTREYYFDY IGHV3-30 IGHD5-18 IGHJ4 ggattcacctttagtaaatattcc atgaactgggtccgccaggctccaggcaaggggctcgagtgggtggcactt atatcatacgatggaagtagaaaa atctacgcagattccgtgaagggccgattcaccatctccagagacaattccaagaacacggtatatctccaaatgagtagcctgagacctgaggacacggctgtctattactgt gcgaggaatgagctgccttgggatacaagagaatactactttgactat IGA1 20 213 8 12 5 13 3 7 5 2 7 6 JY8QFUQ01A5ATC 7762 productive Homsap IGHV1-46*01 F, or Homsap IGHV1-46*03 F 885 90.65 194/214 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-10*01 F 2 8 8 14 8.8.14 [0.17.38.11] CARELGGSGTCGFDYW in-frame + tggatacaccttcaccaggcactatatgcactgggtgcgacaggcccctggacaaggacttgagtacatgggagtaatcaaccctagtggtggcgacacaagctacgcacagaggttccggggcagagtcgccgtgaccagagacacgtccacgagcacagtctatatggacttgagcagcctgagacctgaggacacggccatgtattattgtgcgagagagttgggaggttcggggacttgcggctttgactactggggccagggaagcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 79 95 [287, 286] c108>g,S36>R(- - -)|t109>c,Y37>H(+ - -)|g147>a|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|a163>g,I55>V(+ - +)|t189>c|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a209>g,K70>R(+ + +)|a215>g,Q72>R(+ - -)|a229>g,T77>A(- - -)|a232>g,M78>V(+ - -)|g240>a|c264>t|g270>c,E90>D(+ - +)|c271>t|t286>c,S96>P(+ - -)|g301>a,V101>M(+ - -)|c309>t| c108>g,S36>R(- - -)|t109>c,Y37>H(+ - -)| g147>a|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|a163>g,I55>V(+ - +)| t189>c|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a209>g,K70>R(+ + +)|a215>g,Q72>R(+ - -)|a229>g,T77>A(- - -)|a232>g,M78>V(+ - -)|g240>a|c264>t|g270>c,E90>D(+ - +)|c271>t|t286>c,S96>P(+ - -)|g301>a,V101>M(+ - -)|c309>t| 320 222 202 20 6 14 6 5 3 3 0 0 0 0 2 1 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 1 0 0 0 0 0 1 0 0 51 51 47 4 1 3 1 2 0 0 0 0 0 0 1 0 0 0 30 24 21 3 1 2 1 1 0 1 0 0 0 0 0 0 0 0 117 114 103 11 4 7 4 2 3 1 0 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,162-163(FR2)|ta,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,62-63(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,164-165(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggca,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|aacc,169-172(CDR2)|agct,196-199(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcaccaggcactat atgcactgggtgcgacaggcccctggacaaggacttgagtacatgggagta atcaaccctagtggtggcgacaca agctacgcacagaggttccggggcagagtcgccgtgaccagagacacgtccacgagcacagtctatatggacttgagcagcctgagacctgaggacacggccatgtattattgt gcgagagagttgggaggttcggggacttgcggctttgactac ARELGGSGTCGFDY IGHV1-46 IGHD3-10 IGHJ4 ggatacaccttcaccaggcactat atgcactgggtgcgacaggcccctggacaaggacttgagtacatgggagta atcaaccctagtggtggcgacaca agctacgcacagaggttccggggcagagtcgccgtgaccagagacacgtccacgagcacagtctatatggacttgagcagcctgagacctgaggacacggccatgtattattgt gcgagagagttgggaggttcggggacttgcggctttgactac IGA2 20 213 17 3 8 11 9 9 5 1 10 4 JY8QFUQ01A5BO8 5283 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 940 93.06 201/216 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [1.17.38.11] CTRDIGGRYSYW in-frame + tccggattcacctttagtagttactggatgcactgggtccgccaagctccaggggagggactggtgtgggtctcacggactaatgaagatgggagtatcacaaactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgtacaagagacatcggtgggcgctactcctactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaaggatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 89 [277, 278] t78>c|c90>t|c108>t|a142>g,K48>E(+ - -)|g147>a|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|g313>a,A105>T(- - -)| t78>c| c90>t|c108>t| a142>g,K48>E(+ - -)|g147>a|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)| g313>a,A105>T(- - -)| 320 224 208 16 5 11 3 5 2 2 0 0 0 1 0 1 1 1 78 3 2 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 22 2 2 0 0 0 2 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 1 1 0 0 0 0 0 0 0 0 0 1 30 24 19 5 0 5 1 1 0 1 0 0 0 1 0 0 1 0 117 114 110 4 0 4 1 2 0 0 0 0 0 0 0 1 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tcc ggattcacctttagtagttactgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt acaagagacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttagtagttactgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt acaagagacatcggtgggcgctactcctac IGA2 14 213 10 4 6 8 4 6 2 2 5 5 JY8QFUQ01A5C4M 20200 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 739 82.19 180/219 nt 81.28 178/219 nt Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD2-21*02 F 2 8 8 15 8.8.15 [2.17.38.11] CARYRVGDSLYYGLDGW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (82,19% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR2-IMGT at codon 47 of V-REGION: 'G' (1 nucleotides at position 56 to 56 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 59 of V-REGION: 'ACT' (3 nucleotides at position 92 to 94 in the user submitted sequence), (do not cause frameshift); gcctctggattcatttttagaaattttgccatgagttggctccgccaggcaccagGggaaggggctggaatgggtctcgactatcagcagcACTagtggtgacacggcatattactcagactccgtgaggggccgcttttccatctccagagacaactccaagagcactctgttcttgcagatgaacagcctgagtgccgaagacacggccatttactactgtgcgagataccgtgtgggtgacagtctctactacggtttggacggctggggccaagggaccacggtcaccgtctcgtcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccca NA IGA1 100 100 [299] c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|c105>a,S35>R(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a110>t,Y37>F(- + -)|c120>t|g124>c,V42>L(+ - +)|t135>a|g153>a|a162>g|g163>a,A55>T(- - -)|t168>c|t171>c|g172>a,G58>S(+ + -)|t174>c,G58>S(+ + -)|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|g191>c,S64>T(+ - +)|c192>g,S64>T(+ - +)|a193>g,T65>A(- - -)|c198>t|g202>t,A68>S(- + -)|a215>g,K72>R(+ + +)|g225>c|c228>t|a229>t,T77>S(+ - +)|t246>c|a254>g,N85>S(- - -)|g258>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|a270>g|a285>t,R95>S(- - -)|g291>a|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)|t306>c| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|c105>a,S35>R(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a110>t,Y37>F(- + -)| c120>t|g124>c,V42>L(+ - +)|t135>a|g153>a|a162>g|g163>a,A55>T(- - -)| t168>c|t171>c|g172>a,G58>S(+ + -)|t174>c,G58>S(+ + -)|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|g191>c,S64>T(+ - +)|c192>g,S64>T(+ - +)|a193>g,T65>A(- - -)| c198>t|g202>t,A68>S(- + -)|a215>g,K72>R(+ + +)|g225>c|c228>t|a229>t,T77>S(+ - +)|t246>c|a254>g,N85>S(- - -)|g258>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|a270>g|a285>t,R95>S(- - -)|g291>a|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)|t306>c| a317>g,K106>R(+ + +)|g319>t| 316 (320) 223 (227) 184 (186) 39 (41) 15 (16) 24 (25) 5 (6) 7 7 7 0 1 5 1 3 1 2 (3) 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 1 3 0 0 1 1 0 0 0 0 0 51 51 45 6 4 2 1 2 1 0 0 0 0 1 1 0 0 0 30 24 15 9 2 7 1 2 0 4 0 0 0 0 1 1 0 0 117 114 96 18 9 9 3 2 3 3 0 0 4 0 1 0 2 0 4 (8) 4 4 (6) 0 (2) 0 (1) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcatttttagaaattttgcc atgagttggctccgccaggcaccagggaaggggctggaatgggtctcgact atcagcagcagtggtgacacggca tattactcagactccgtgaggggccgcttttccatctccagagacaactccaagagcactctgttcttgcagatgaacagcctgagtgccgaagacacggccatttactactgt gcgagataccgtgtgggtgacagtctctactacggtttggacggc ARYRVGDSLYYGLDG IGHV3-23 IGHD2-21 IGHJ6 ggattcatttttagaaattttgcc atgagttggctccgccaggcaccagggaaggggctggaatgggtctcgact atcagcagcagtggtgacacggca tattactcagactccgtgaggggccgcttttccatctccagagacaactccaagagcactctgttcttgcagatgaacagcctgagtgccgaagacacggccatttactactgt gcgagataccgtgtgggtgacagtctctactacggtttggacggc IGA1 39 213 26 13 14 21 12 18 13 2 11 13 JY8QFUQ01A5D4P 12228 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 850 88.43 191/216 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD1-26*01 F 2 8 8 14 8.8.14 [1.17.38.11] CAREVPHQNIIYLDYW in-frame - tctggattcgactttagcagcgcttccatggcctgggtccgccaggctccagggaaggggctggagtggatctcagctgtcagtggacgtggtgacaacaccttctacgcagcctccgtgaagggccggttcaccatctccagagaccattccaagaacacgctgtggcttcaaatgaacaacctgagagccgaagacacggccctatattactgtgcgagggaagtcccacatcaaaatataatctaccttgactactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [289] a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|t109>g,Y37>A(- - -)|a110>c,Y37>A(- - -)|g112>t,A38>S(- + -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g157>a,V53>I(+ - +)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|t174>a|a175>c,S59>R(- - -)|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|g191>a,S64>N(- - -)|a195>c|a197>t,Y66>F(- + -)|a206>c,D69>A(- - -)|a244>c,N82>H(- - -)|a263>g,Y88>W(- + -)|t264>g,Y88>W(- + -)|g267>t|g278>a,S93>N(- - -)|g291>a|g301>c,V101>L(+ - +)| a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|t109>g,Y37>A(- - -)|a110>c,Y37>A(- - -)|g112>t,A38>S(- + -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g157>a,V53>I(+ - +)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|t174>a|a175>c,S59>R(- - -)|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|g191>a,S64>N(- - -)|a195>c| a197>t,Y66>F(- + -)|a206>c,D69>A(- - -)|a244>c,N82>H(- - -)|a263>g,Y88>W(- + -)|t264>g,Y88>W(- + -)|g267>t|g278>a,S93>N(- - -)|g291>a|g301>c,V101>L(+ - +)| a317>g,K106>R(+ + +)|a318>g,K106>R(+ + +)| 316 (320) 220 (224) 195 (197) 25 (27) 4 21 (23) 4 (6) 5 0 2 5 1 1 1 2 0 2 2 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 0 0 0 1 1 0 0 0 0 1 1 51 51 48 3 0 3 1 1 0 0 0 0 0 0 1 0 0 0 30 24 16 8 2 6 1 2 0 2 2 0 0 1 0 0 0 0 117 114 105 9 2 7 1 2 0 0 2 0 1 0 1 0 1 1 4 (8) 4 4 (6) 0 (2) 0 0 (2) 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcgactttagcagcgcttcc atggcctgggtccgccaggctccagggaaggggctggagtggatctcagct gtcagtggacgtggtgacaacacc ttctacgcagcctccgtgaagggccggttcaccatctccagagaccattccaagaacacgctgtggcttcaaatgaacaacctgagagccgaagacacggccctatattactgt gcgagggaagtcccacatcaaaatataatctaccttgactac AREVPHQNIIYLDY IGHV3-23 IGHD1-26 IGHJ4 ggattcgactttagcagcgcttcc atggcctgggtccgccaggctccagggaaggggctggagtggatctcagct gtcagtggacgtggtgacaacacc ttctacgcagcctccgtgaagggccggttcaccatctccagagaccattccaagaacacgctgtggcttcaaatgaacaacctgagagccgaagacacggccctatattactgt gcgagggaagtcccacatcaaaatataatctaccttgactac IGA1 25 213 11 14 5 10 6 15 2 2 10 11 JY8QFUQ01A5FS2 20478 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 1035 97.7 212/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD6-25*01 F 1 8 8 13 8.8.13 [1.17.38.11] CARDLTGYSSERNYW in-frame + ctctggattcaccttcagtaactatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattagtagtagtgctaggtacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgcgagacctgaccgggtatagcagcgaacgaaactactggggccagggaaccctggtcaccgtctcctcagcatcccccgaccagccccaaaggtcttcccgctgagctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 86 74 [288] g107>a,S36>N(- - -)|a184>g,S62>A(- + -)|g185>c,S62>A(- + -)|t189>g,S63>R(- - -)|a263>c,Y88>S(+ - -)|a316>c| g107>a,S36>N(- - -)| a184>g,S62>A(- + -)|g185>c,S62>A(- + -)|t189>g,S63>R(- - -)| a263>c,Y88>S(+ - -)| a316>c| 320 225 219 6 1 5 1 1 0 0 2 0 0 0 1 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 1 0 0 1 117 114 113 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 8 8 7 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtaactatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtgctaggtacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgcgagacctgaccgggtatagcagcgaacgaaactac ARDLTGYSSERNY IGHV3-21 IGHD6-25 IGHJ4 ggattcaccttcagtaactatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtgctaggtacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgcgagacctgaccgggtatagcagcgaacgaaactac IGA2 5 213 2 3 1 2 1 3 0 0 1 4 JY8QFUQ01A5FUX 20970 productive (see comment) Homsap IGHV3-30*01 F 869 88.99 194/218 nt 88.53 193/218 nt Homsap IGHJ6*02 F (see comment) 184 77.42 48/62 nt Homsap IGHD3-10*01 F 3 8 8 18 8.8.18 [1.17.38.11] CARGTMLRGVTKARYGLDVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) in CDR1-IMGT at codon 30 of V-REGION: 'T' (1 nucleotides at position 16 to 16 in the user submitted sequence), (cause frameshift); cctctggattcacctTtcagtacctatgctatgtactgggtccgccaggctccaggcaaggggccagagtgggtgtcagtgatatcacatgatggaaataaggaagaatacgcagactccgtgaagggccgattcaccatttccagagacaactccaagaaaatgttgtacctgcaaatgaacaaccagcgacctgatgacacggctgtttattattgtgcgagagggactatgcttcggggagtcaccaaagcgagatacggtctggacgtctggggccaagggaccacggtcatcgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 93 100 [305] g107>c,S36>T(+ - +)|c118>t,H40>Y(+ - -)|t149>c,L50>P(- - -)|g160>t,A54>S(- + -)|t165>g|t172>c,Y58>H(+ - -)|g188>a,S63>N(- - -)|t192>g,N64>K(+ - -)|a193>g,K65>E(+ - -)|t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|c234>t|t246>c|c255>a,N85>K(+ - -)|c257>t,T86>M(- - -)|c259>t|t264>c|g278>a,S93>N(- - -)|t281>a,L94>Q(- - -)|a283>c|g286>c,A96>P(- - -)|g291>t,E97>D(+ - +)|g303>t|c309>t| g107>c,S36>T(+ - +)| c118>t,H40>Y(+ - -)|t149>c,L50>P(- - -)|g160>t,A54>S(- + -)|t165>g| t172>c,Y58>H(+ - -)|g188>a,S63>N(- - -)|t192>g,N64>K(+ - -)|a193>g,K65>E(+ - -)| t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|c234>t|t246>c|c255>a,N85>K(+ - -)|c257>t,T86>M(- - -)|c259>t|t264>c|g278>a,S93>N(- - -)|t281>a,L94>Q(- - -)|a283>c|g286>c,A96>P(- - -)|g291>t,E97>D(+ - +)|g303>t|c309>t| a320>g| 319 (320) 225 (226) 201 24 (25) 8 (9) 16 1 (2) 2 5 4 1 2 0 1 2 0 3 3 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 47 4 1 3 0 0 1 1 0 0 0 0 0 0 1 1 30 24 20 4 0 4 1 1 0 1 0 0 0 0 0 0 0 1 117 114 99 15 7 8 0 1 4 2 1 2 0 1 1 0 2 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtacctatgct atgtactgggtccgccaggctccaggcaaggggccagagtgggtgtcagtg atatcacatgatggaaataaggaa gaatacgcagactccgtgaagggccgattcaccatttccagagacaactccaagaaaatgttgtacctgcaaatgaacaaccagcgacctgatgacacggctgtttattattgt gcgagagggactatgcttcggggagtcaccaaagcgagatacggtctggacgtc ARGTMLRGVTKARYGLDV IGHV3-30 IGHD3-10 IGHJ6 ggattcaccttcagtacctatgct atgtactgggtccgccaggctccaggcaaggggccagagtgggtgtcagtg atatcacatgatggaaataaggaa gaatacgcagactccgtgaagggccgattcaccatttccagagacaactccaagaaaatgttgtacctgcaaatgaacaaccagcgacctgatgacacggctgtttattattgt gcgagagggactatgcttcggggagtcaccaaagcgagatacggtctggacgtc IGG2 24 213 12 12 7 14 5 10 8 0 11 5 JY8QFUQ01A5GJK 12127 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 954 93.55 203/217 nt 93.09 202/217 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD6-13*01 F 3 8 8 11 8.8.11 [1.17.38.11] CVRDRSGQAVGYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 12 to 12 in the user submitted sequence), (cause frameshift); ctctggattcaGccttcagtagttactggatgcactgggtccgccaagttccagggaagggactggtgtgggtctcacgaattaatactgatgggagtgccacaagttacgcggactccgtgaggggccgattcaccatctccagagacaacgccaagaacacgctatatcttcaaatgaacagtctgagagtcgaagacacggctgtctattactgtgtaagagatcggtccggtcaggcggtaggatactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [283] c108>t|c134>t,A45>V(+ - +)|g147>a|t165>a|g173>c,S58>T(+ - +)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|c198>t|a215>g,K72>R(+ + +)|g261>a|g267>t|c287>t,A96>V(+ - +)|g291>a|g303>c|c314>t,A105>V(+ - +)| c108>t| c134>t,A45>V(+ - +)|g147>a|t165>a| g173>c,S58>T(+ - +)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| c198>t|a215>g,K72>R(+ + +)|g261>a|g267>t|c287>t,A96>V(+ - +)|g291>a|g303>c| c314>t,A105>V(+ - +)| 320 225 210 15 8 7 2 3 5 0 0 0 0 1 3 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 1 0 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 2 0 0 0 117 114 107 7 5 2 1 2 2 0 0 0 0 0 1 0 1 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagttactgg atgcactgggtccgccaagttccagggaagggactggtgtgggtctcacga attaatactgatgggagtgccaca agttacgcggactccgtgaggggccgattcaccatctccagagacaacgccaagaacacgctatatcttcaaatgaacagtctgagagtcgaagacacggctgtctattactgt gtaagagatcggtccggtcaggcggtaggatac VRDRSGQAVGY IGHV3-74 IGHD6-13 IGHJ4 ggattcaccttcagtagttactgg atgcactgggtccgccaagttccagggaagggactggtgtgggtctcacga attaatactgatgggagtgccaca agttacgcggactccgtgaggggccgattcaccatctccagagacaacgccaagaacacgctatatcttcaaatgaacagtctgagagtcgaagacacggctgtctattactgt gtaagagatcggtccggtcaggcggtaggatac IGG2 14 213 9 5 7 11 2 3 7 1 3 3 JY8QFUQ01A5HXY 5249 productive Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F or Homsap IGHV4-34*12 F 826 87.79 187/213 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-16*01 F 3 8 7 14 8.7.14 [1.17.38.11] CARKGLVPSMFFFDFW in-frame - tctggtgggtccttcagtggttacttctggaactggatccgccagcccccagggaaggggccggagtggattggagaagtcagtcatgatggaagtaccaacttcaatccgtccctcaagagtcgagtctccatgtcagttgacacgatcaagaagcaggtcttcctgaaactgagctctgtgactgccggggacacggctatatattattgtgcgagaaagggacttgtgccatctatgttcttctttgacttctggggccagggatccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 100 100 [287] a77>c,Y26>S(+ - -)|a113>t,Y38>F(- + -)|g119>a,S40>N(- - -)|t149>c,L50>P(- - -)|g162>a|a166>g,I56>V(+ - +)|a170>g,N57>S(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|c192>t|a200>t,Y67>F(- + -)|c204>t|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|a240>t|t247>a,S83>I(- - -)|c248>t,S83>I(- - -)|c255>g,N85>K(+ - -)|t259>g,F87>V(+ - -)|c263>t,S88>F(- - -)|g270>a|c285>t|c290>g,A97>G(- + -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| a77>c,Y26>S(+ - -)| a113>t,Y38>F(- + -)| g119>a,S40>N(- - -)|t149>c,L50>P(- - -)|g162>a| a166>g,I56>V(+ - +)|a170>g,N57>S(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|c192>t| a200>t,Y67>F(- + -)|c204>t|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|a240>t|t247>a,S83>I(- - -)|c248>t,S83>I(- - -)|c255>g,N85>K(+ - -)|t259>g,F87>V(+ - -)|c263>t,S88>F(- - -)|g270>a|c285>t|c290>g,A97>G(- + -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| g319>a|g320>a| 318 (320) 219 (221) 193 26 (28) 7 (9) 19 4 6 (8) 6 1 1 0 4 1 0 2 0 1 78 3 2 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 51 51 48 3 1 2 0 2 0 1 0 0 0 0 0 0 0 0 30 21 16 5 1 4 3 1 1 0 0 0 0 0 0 0 0 0 117 114 98 16 5 11 1 3 5 0 0 0 3 1 0 2 0 1 6 (8) 6 6 0 (2) 0 (2) 0 0 0 (2) 0 0 0 0 0 0 0 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tct ggtgggtccttcagtggttacttc tggaactggatccgccagcccccagggaaggggccggagtggattggagaa gtcagtcatgatggaagtacc aacttcaatccgtccctcaagagtcgagtctccatgtcagttgacacgatcaagaagcaggtcttcctgaaactgagctctgtgactgccggggacacggctatatattattgt gcgagaaagggacttgtgccatctatgttcttctttgacttc ARKGLVPSMFFFDF IGHV4-34 IGHD3-16 IGHJ4 ggtgggtccttcagtggttacttc tggaactggatccgccagcccccagggaaggggccggagtggattggagaa gtcagtcatgatggaagtacc aacttcaatccgtccctcaagagtcgagtctccatgtcagttgacacgatcaagaagcaggtcttcctgaaactgagctctgtgactgccggggacacggctatatattattgt gcgagaaagggacttgtgccatctatgttcttctttgacttc IGG1 25 210 17 8 12 14 5 11 6 1 13 5 JY8QFUQ01A5I8W 18957 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 986 94.95 207/218 nt NA Homsap IGHJ4*02 F 141 77.08 37/48 nt Homsap IGHD1-26*01 F 1 8 8 9 8.8.9 [1.17.38.11] CTREVGATDYW in-frame - cctctggattcaccttcagtagctactggatgcattgggtccgccaagctccagggaaggggctggagtgggtctcacgtattcatagtgatgggactaccacatactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaggaacacgttgtatctgcaattgaacagtctgagagccgaggacacggctgtgtattattgtacaagagaggtgggagctaccgactactggggccagggaactctggtcaccgttacctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [276] c120>t|t152>a,V51>E(- + -)|a169>c,N57>H(- - -)|g188>c,S63>T(+ - +)|g191>c,S64>T(+ - +)|a196>t,S66>Y(+ - -)|g197>a,S66>Y(+ - -)|a251>g,K84>R(+ + +)|c259>t|a271>t,M91>L(+ + -)|c309>t|g313>a,A105>T(- - -)| c120>t|t152>a,V51>E(- + -)| a169>c,N57>H(- - -)|g188>c,S63>T(+ - +)|g191>c,S64>T(+ - +)| a196>t,S66>Y(+ - -)|g197>a,S66>Y(+ - -)|a251>g,K84>R(+ + +)|c259>t|a271>t,M91>L(+ + -)|c309>t| g313>a,A105>T(- - -)| 320 226 214 12 3 9 1 2 3 0 1 0 2 1 2 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 0 1 0 0 0 0 1 0 0 0 0 30 24 21 3 0 3 0 0 0 0 1 0 0 0 2 0 0 0 117 114 108 6 2 4 1 1 2 0 0 0 2 0 0 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtagctactgg atgcattgggtccgccaagctccagggaaggggctggagtgggtctcacgt attcatagtgatgggactaccaca tactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaggaacacgttgtatctgcaattgaacagtctgagagccgaggacacggctgtgtattattgt acaagagaggtgggagctaccgactac TREVGATDY IGHV3-74 IGHD1-26 IGHJ4 ggattcaccttcagtagctactgg atgcattgggtccgccaagctccagggaaggggctggagtgggtctcacgt attcatagtgatgggactaccaca tactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaggaacacgttgtatctgcaattgaacagtctgagagccgaggacacggctgtgtattattgt acaagagaggtgggagctaccgactac IGA1 11 213 5 6 4 6 1 5 3 0 5 3 JY8QFUQ01A5IKH 20542 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 896 90.37 197/218 nt 89.91 196/218 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD4-23*01 ORF 1 7 8 10 7.8.10 [2.17.38.11] CARLGQLALDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 29 of V-REGION: 1 nucleotides (from position 13 in the user submitted sequence), (cause frameshift) tgttcgggattcgctttaccacgtactggatcggctgggtgcgccagatgcccgggaagggcctggagttgatgggaatcatctttcctggtgactctgaggccagatacagcccgtccttccaaggccaggtcaccctctcagccgacacgtccaccaccaccgtctatctgcagtggagcagtctgaggacctcggacaccgccgtgtattactgtgcgagacttgggcaactcgctcttgactactggggcgagggaaccctgatttccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [280] g73>t,G25>C(- - -)|t78>g|a83>t,Y28>F(- + -)|g107>c,S36>T(+ - +)|c108>g,S36>T(+ - +)|a144>g|g155>t,W52>L(+ - -)|g162>a|a170>t,Y57>F(- + -)|t192>g,D64>E(+ - +)|a193>g,T65>A(- - -)|a232>c,I78>L(+ + +)|a245>c,K82>T(- - -)|t251>c,I84>T(- - -)|g254>c,S85>T(+ - +)|c260>t,A87>V(+ - +)|c264>t|c279>t|a284>g,K95>R(+ + +)|g286>a,A96>T(- - -)|a301>g,M101>V(+ - -)| g73>t,G25>C(- - -)|t78>g| a83>t,Y28>F(- + -)|g107>c,S36>T(+ - +)|c108>g,S36>T(+ - +)| a144>g|g155>t,W52>L(+ - -)|g162>a| a170>t,Y57>F(- + -)|t192>g,D64>E(+ - +)|a193>g,T65>A(- - -)| a232>c,I78>L(+ + +)|a245>c,K82>T(- - -)|t251>c,I84>T(- - -)|g254>c,S85>T(+ - +)|c260>t,A87>V(+ - +)|c264>t|c279>t|a284>g,K95>R(+ + +)|g286>a,A96>T(- - -)|a301>g,M101>V(+ - -)| a320>t| 319 (320) 225 (226) 204 21 (22) 5 (6) 16 4 2 3 1 2 0 2 (3) 0 2 1 2 2 78 6 4 2 1 1 0 0 0 0 0 0 0 0 0 0 1 1 36 23 20 3 0 3 0 0 0 0 0 0 1 0 1 1 0 0 51 51 48 3 2 1 1 1 0 0 0 0 0 0 0 0 1 0 30 24 21 3 0 3 1 0 0 0 0 0 1 0 0 0 0 1 117 114 104 10 2 8 2 1 3 1 2 0 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| tgttcg ggattcgctttaccacgtactgg atcggctgggtgcgccagatgcccgggaagggcctggagttgatgggaatc atctttcctggtgactctgaggcc agatacagcccgtccttccaaggccaggtcaccctctcagccgacacgtccaccaccaccgtctatctgcagtggagcagtctgaggacctcggacaccgccgtgtattactgt gcgagacttgggcaactcgctcttgactac ARLGQLALDY IGHV5-51 IGHD4-23 IGHJ4 ggattcgctttaccacgtactgg atcggctgggtgcgccagatgcccgggaagggcctggagttgatgggaatc atctttcctggtgactctgaggcc agatacagcccgtccttccaaggccaggtcaccctctcagccgacacgtccaccaccaccgtctatctgcagtggagcagtctgaggacctcggacaccgccgtgtattactgt gcgagacttgggcaactcgctcttgactac IGG1 19 212 10 9 5 9 5 10 4 0 9 6 JY8QFUQ01A5IOF 2159 productive Homsap IGHV3-11*01 F (see comment) 774 84.33 183/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD5-12*01 F 1 8 8 10 8.8.10 [1.17.38.11] CARDLVGSIGSW in-frame + Low V-REGION identity (84,33% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctggattcacatttggtgactacgtcatgacttggatccgccaggctccagggaaggggctggagtggatttcgtacatcaccactgatggttctttgatagaatatgcagaatctgtgaagggccggttcaccatttccagggacaacgccaagaactcactgtggctgcagatggacaacctgagagtcgacgacacggccgtttattactgtgcgagggacctcgtgggttccattgggtcctggggccagggaaccctggtcgtcgtctcctcagcctccaccaagggcccatcggtcttcccccgtggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 93 88 [280, 279] c87>a|c90>t|a103>g,S35>G(+ + -)|t112>g,Y38>V(- - -)|a113>t,Y38>V(- - -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g157>a,V53>I(+ - +)|a162>g|t168>c|g170>c,S57>T(+ - +)|t171>c,S57>T(+ - +)|g173>c,S58>T(+ - +)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|a187>t|g188>c|a190>t,T64>L(- - -)|c191>t,T64>L(- - -)|c192>g,T64>L(- - -)|t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|c201>t|c207>a,D69>E(+ - +)|a225>g|c234>t|a263>g,Y88>W(- + -)|t264>g,Y88>W(- + -)|a270>g|a274>g,N92>D(+ + -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>t|a318>g| c87>a|c90>t|a103>g,S35>G(+ + -)|t112>g,Y38>V(- - -)|a113>t,Y38>V(- - -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g157>a,V53>I(+ - +)|a162>g| t168>c|g170>c,S57>T(+ - +)|t171>c,S57>T(+ - +)|g173>c,S58>T(+ - +)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|a187>t|g188>c|a190>t,T64>L(- - -)|c191>t,T64>L(- - -)|c192>g,T64>L(- - -)| t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|c201>t|c207>a,D69>E(+ - +)|a225>g|c234>t|a263>g,Y88>W(- + -)|t264>g,Y88>W(- + -)|a270>g|a274>g,N92>D(+ + -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>t| a318>g| 320 225 190 35 12 23 8 3 6 2 0 3 3 0 5 1 1 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 1 0 1 0 0 1 1 0 0 0 0 1 51 51 47 4 1 3 1 1 1 0 0 0 0 0 1 0 0 0 30 24 13 11 3 8 1 1 1 2 0 0 2 0 3 1 0 0 117 114 100 14 5 9 4 1 3 0 0 2 0 0 1 0 1 2 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacatttggtgactacgtc atgacttggatccgccaggctccagggaaggggctggagtggatttcgtac atcaccactgatggttctttgata gaatatgcagaatctgtgaagggccggttcaccatttccagggacaacgccaagaactcactgtggctgcagatggacaacctgagagtcgacgacacggccgtttattactgt gcgagggacctcgtgggttccattgggtcc ARDLVGSIGS IGHV3-11 IGHD5-12 IGHJ4 ggattcacatttggtgactacgtc atgacttggatccgccaggctccagggaaggggctggagtggatttcgtac atcaccactgatggttctttgata gaatatgcagaatctgtgaagggccggttcaccatttccagggacaacgccaagaactcactgtggctgcagatggacaacctgagagtcgacgacacggccgtttattactgt gcgagggacctcgtgggttccattgggtcc IGG1 34 213 18 16 9 19 9 15 6 5 12 11 JY8QFUQ01A5JVK 18798 productive Homsap IGHV3-48*01 F 950 93.12 203/218 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 3 8 8 12 8.8.12 [1.17.38.11] CARDTGQWKDFDYW in-frame + cctctggattcgccttcagtagttccagcatgaactgggtccgccagggtccagggaaggggctggagtggatttcacacattaggggtagtagtagtaccacccactacgcagactctgtgaagggccggttcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgtgcaagagatacggggcagtggaaggactttgactactggggccagggaaccctggtcaccgtctccttagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [285] a85>g,T29>A(- - -)|c108>t|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)|c134>g,A45>G(- + -)|g157>a,V53>I(+ - +)|t163>c,Y55>H(+ - -)|t171>g,S57>R(- - -)|a172>g,S58>G(+ + -)|t194>c,I65>T(- - -)|a195>c,I65>T(- - -)|t196>c,Y66>H(+ - -)|a225>g|c279>t|g303>t|g315>a| a85>g,T29>A(- - -)|c108>t|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)| c134>g,A45>G(- + -)|g157>a,V53>I(+ - +)|t163>c,Y55>H(+ - -)| t171>g,S57>R(- - -)|a172>g,S58>G(+ + -)|t194>c,I65>T(- - -)|a195>c,I65>T(- - -)| t196>c,Y66>H(+ - -)|a225>g|c279>t|g303>t| g315>a| 320 226 210 16 5 11 3 2 2 4 2 0 0 0 0 1 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 1 1 1 0 0 0 0 0 0 0 51 51 48 3 0 3 0 1 0 1 0 0 0 0 0 1 0 0 30 24 20 4 0 4 1 0 0 1 1 0 0 0 0 0 0 1 117 114 110 4 3 1 1 0 1 1 0 0 0 0 0 0 1 0 8 8 7 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcgccttcagtagttccagc atgaactgggtccgccagggtccagggaaggggctggagtggatttcacac attaggggtagtagtagtaccacc cactacgcagactctgtgaagggccggttcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac ARDTGQWKDFDY IGHV3-48 IGHD6-19 IGHJ4 ggattcgccttcagtagttccagc atgaactgggtccgccagggtccagggaaggggctggagtggatttcacac attaggggtagtagtagtaccacc cactacgcagactctgtgaagggccggttcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac IGA1 15 213 10 5 3 5 7 10 3 1 4 7 JY8QFUQ01A5MH8 13392 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 980 95.35 205/215 nt 94.88 204/215 nt Homsap IGHJ4*02 F (see comment) 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 8 19 8.8.19 [1.16.38.11] CAKDGQRYCSNGVCSSFFDSW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR2-IMGT, from codon 41 of V-REGION: 1 nucleotides (from position 36 in the user submitted sequence), (cause frameshift) tctggattcacctacagcagctatgccatgagctggtccgccaggctccagggaaggggctggagtgggtctcagcaattagtggtggtggtgctagtacataccacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatttgcaaatgaacagcctgagagccgacgacacggccgtatattactgtgcgaaagatggacaacgatattgctctaatggtgtatgctcgtccttctttgactcctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [304] t89>a,F30>Y(- + -)|t90>c,F30>Y(- + -)|t165>a|a175>g,S59>G(+ + -)|g188>c,G63>A(- + -)|c192>t|t199>c,Y67>H(+ - -)|g225>a|c265>t|g291>c,E97>D(+ - +)| t89>a,F30>Y(- + -)|t90>c,F30>Y(- + -)| t165>a| a175>g,S59>G(+ + -)|g188>c,G63>A(- + -)|c192>t| t199>c,Y67>H(+ - -)|g225>a|c265>t|g291>c,E97>D(+ - +)| 320 223 213 10 4 6 1 1 2 2 0 0 0 2 2 0 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 1 0 0 0 1 0 0 0 0 51 50 49 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 24 21 3 1 2 1 0 1 0 0 0 0 0 1 0 0 0 117 114 110 4 2 2 0 1 1 1 0 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcacctacagcagctatgcc atgagctggtccgccaggctccagggaaggggctggagtgggtctcagca attagtggtggtggtgctagtaca taccacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatttgcaaatgaacagcctgagagccgacgacacggccgtatattactgt gcgaaagatggacaacgatattgctctaatggtgtatgctcgtccttctttgactcc AKDGQRYCSNGVCSSFFDS IGHV3-23 IGHD2-8 IGHJ4 ggattcacctacagcagctatgcc atgagctggtccgccaggctccagggaaggggctggagtgggtctcagca attagtggtggtggtgctagtaca taccacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatttgcaaatgaacagcctgagagccgacgacacggccgtatattactgt gcgaaagatggacaacgatattgctctaatggtgtatgctcgtccttctttgactcc IGG1 10 212 6 4 3 5 3 5 3 1 2 4 JY8QFUQ01A5NL9 9385 productive (see comment) Homsap IGHV1-3*01 F 840 88.32 189/214 nt 87.38 187/214 nt Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 174 82.35 42/51 nt Homsap IGHD2-8*02 F 2 8 8 13 8.8.13 [0.17.38.11] CARDGYLPGNGADFW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 44 of V-REGION: 'G' (1 nucleotides at position 43 to 43 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 54 of V-REGION: 'G' (1 nucleotides at position 73 to 73 in the user submitted sequence), (cause frameshift); tggatacagcttcactaatcacattatccattgggtgcgccaGggcccccggacaagggcttgagtgggtggGggtcgatcaacgctggcaatggcaatacaagatattcacagaagttgcagggcagagtcaccatttccagggacacatccgcgagcatcgccaacatggagttgagcagtctgagatatgaagacacggctgtatattattgtgcgagagacggctatttgcctggcaacggggctgacttttggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccactgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [286] c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)|g112>a,A38>I(+ - +)|c113>t,A38>I(+ - +)|g117>c,M39>I(+ + -)|a145>g,R49>G(- - -)|a157>g,M53>V(+ - -)|a162>g|g164>c,W55>S(- - -)|t189>c|c192>t|a197>g,K66>R(+ + +)|c213>g,F71>L(+ - -)|a235>t,T79>S(+ - +)|c257>t,T86>I(- - -)|a258>c,T86>I(- - -)|t262>a,Y88>N(- - -)|c271>t|c279>t|c287>a,S96>Y(+ - -)|g303>a|c309>t| c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)|g112>a,A38>I(+ - +)|c113>t,A38>I(+ - +)| g117>c,M39>I(+ + -)|a145>g,R49>G(- - -)|a157>g,M53>V(+ - -)|a162>g|g164>c,W55>S(- - -)| t189>c|c192>t| a197>g,K66>R(+ + +)|c213>g,F71>L(+ - -)|a235>t,T79>S(+ - +)|c257>t,T86>I(- - -)|a258>c,T86>I(- - -)|t262>a,Y88>N(- - -)|c271>t|c279>t|c287>a,S96>Y(+ - -)|g303>a|c309>t| 320 222 197 25 7 18 4 3 7 3 1 1 1 1 2 2 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 0 7 0 2 2 2 0 0 0 0 0 1 0 0 51 51 46 5 1 4 3 0 0 0 0 0 0 0 2 0 0 0 30 24 22 2 2 0 0 0 1 1 0 0 0 0 0 0 0 0 117 114 103 11 4 7 1 1 4 0 1 1 1 1 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|ta,262-263(FR3)|aa,290-291(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,11-12(FR1)|tt,62-63(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,120-121(FR2)|tt,149-150(FR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,201-202(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacagcttcactaatcacatt atccattgggtgcgccaggcccccggacaagggcttgagtgggtggggtcg atcaacgctggcaatggcaataca agatattcacagaagttgcagggcagagtcaccatttccagggacacatccgcgagcatcgccaacatggagttgagcagtctgagatatgaagacacggctgtatattattgt gcgagagacggctatttgcctggcaacggggctgacttt ARDGYLPGNGADF IGHV1-3 IGHD2-8 IGHJ5 ggatacagcttcactaatcacatt atccattgggtgcgccaggcccccggacaagggcttgagtgggtggggtcg atcaacgctggcaatggcaataca agatattcacagaagttgcagggcagagtcaccatttccagggacacatccgcgagcatcgccaacatggagttgagcagtctgagatatgaagacacggctgtatattattgt gcgagagacggctatttgcctggcaacggggctgacttt IGA1 25 213 17 8 10 15 7 10 5 2 11 7 JY8QFUQ01A5OJ5 7360 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 846 88.02 191/217 nt NA Homsap IGHJ6*01 F (see comment) 171 74.6 47/63 nt Homsap IGHD2-2*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVSDRELHYQHLDVW in-frame + Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) gtctggattcaacttcaatagttttggcatgcactgggtccgccaggctccgggcaagggactggagtgggtggcaaacatatggtatgatggaggtagtcaacactatgcagacctcgtgaagggccgattcaccatctctagagacaattccaagaacatcttgttcctgcaaatgagcgacctgagagccgaggacacggctgtttattattgtgtgagcgatagagaactccattaccagcatttggacgtctggggtcaagggaccacggtcatcgtctcatcagcctccaccaagggcccatcggtcttccccctggcaccctctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 79 88 [288, 287] c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|c108>t|a110>t,Y37>F(- + -)|a138>g|g147>a|g163>a,V55>N(- - -)|t164>a,V55>N(- - -)|t165>c,V55>N(- - -)|a187>g,S63>G(+ + -)|a191>g,N64>S(- - -)|a193>c,K65>Q(+ - -)|t196>c,Y66>H(+ - -)|t208>c,S70>L(- - -)|c209>t,S70>L(- - -)|c237>t|c257>t,T86>I(- - -)|g258>c,T86>I(- - -)|c259>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|a275>g,N92>S(- - -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g303>t|c309>t|c314>t,A105>V(+ - +)|a318>c,R106>S(- - -)| c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|c108>t|a110>t,Y37>F(- + -)| a138>g|g147>a|g163>a,V55>N(- - -)|t164>a,V55>N(- - -)|t165>c,V55>N(- - -)| a187>g,S63>G(+ + -)|a191>g,N64>S(- - -)|a193>c,K65>Q(+ - -)| t196>c,Y66>H(+ - -)|t208>c,S70>L(- - -)|c209>t,S70>L(- - -)|c237>t|c257>t,T86>I(- - -)|g258>c,T86>I(- - -)|c259>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|a275>g,N92>S(- - -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g303>t|c309>t| c314>t,A105>V(+ - +)|a318>c,R106>S(- - -)| 320 225 197 28 7 21 5 4 7 4 2 1 2 1 1 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 1 0 0 1 1 0 0 0 0 0 51 51 46 5 2 3 1 2 0 1 0 0 0 1 0 0 0 0 30 24 21 3 0 3 2 0 0 0 1 0 0 0 0 0 0 0 117 114 100 14 4 10 2 1 5 3 0 0 1 0 1 0 1 0 8 8 6 2 0 2 0 0 1 0 1 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcaacttcaatagttttggc atgcactgggtccgccaggctccgggcaagggactggagtgggtggcaaac atatggtatgatggaggtagtcaa cactatgcagacctcgtgaagggccgattcaccatctctagagacaattccaagaacatcttgttcctgcaaatgagcgacctgagagccgaggacacggctgtttattattgt gtgagcgatagagaactccattaccagcatttggacgtc VSDRELHYQHLDV IGHV3-33 IGHD2-2 IGHJ6 ggattcaacttcaatagttttggc atgcactgggtccgccaggctccgggcaagggactggagtgggtggcaaac atatggtatgatggaggtagtcaa cactatgcagacctcgtgaagggccgattcaccatctctagagacaattccaagaacatcttgttcctgcaaatgagcgacctgagagccgaggacacggctgtttattattgt gtgagcgatagagaactccattaccagcatttggacgtc IGG1 26 213 19 7 10 13 9 13 6 1 13 6 JY8QFUQ01A5SB0 10737 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-3*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARDCDSGECNFDYW in-frame + ctctggattcagcttcagtacctataacatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccataagtagtggtagtacttacatatatcacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaagacactttacctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagattgtgacagtggtgaatgtaactttgactactggggccagggaaccctggtcaccgtcttctcagcctccaccaagggcccatcggtcttccccctggcaccctctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 79 88 [288, 287] c86>g,T29>S(+ - +)|g107>c,S36>T(+ - +)|g113>a,S38>N(- - -)|t168>a|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|c198>t|t199>c,Y67>H(+ - -)|c255>g,N85>K(+ - -)|t256>a,S86>T(+ - +)|g261>t|t264>c| c86>g,T29>S(+ - +)|g107>c,S36>T(+ - +)|g113>a,S38>N(- - -)| t168>a|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)| c198>t|t199>c,Y67>H(+ - -)|c255>g,N85>K(+ - -)|t256>a,S86>T(+ - +)|g261>t|t264>c| 320 225 213 12 4 8 1 1 1 2 0 0 0 2 2 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 1 0 0 0 0 0 0 1 1 0 0 0 117 114 108 6 3 3 0 0 1 2 0 0 0 1 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagcttcagtacctataac atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc ataagtagtggtagtacttacata tatcacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaagacactttacctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagattgtgacagtggtgaatgtaactttgactac ARDCDSGECNFDY IGHV3-21 IGHD3-3 IGHJ4 ggattcagcttcagtacctataac atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc ataagtagtggtagtacttacata tatcacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaagacactttacctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagattgtgacagtggtgaatgtaactttgactac IGG1 12 213 5 7 2 7 3 5 3 1 3 5 JY8QFUQ01A5U3W 3949 productive Homsap IGHV3-64*02 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-22*01 F 2 8 8 10 8.8.10 [1.17.38.11] CVTCGDYSYEYW in-frame + ctctggattcattttcagtacttatcctatgcactgggtccgccaggctccagggaagggactggaatatgtttcagctattagtcgtaatggggataacgcatattatgcagactctgtgaagggcagattcaccatgtccagagacaattccaagagcacactgtatcttcagatgggcagcctgagagctgaggacatggctgtgtattactgtgtgacatgtggtgattacagctatgagtactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgagaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [278] c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>c,A38>P(- - -)|a172>c,S58>R(- - -)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|a193>g,T65>A(- - -)|c234>g,I78>M(+ + -)|a254>g,N85>S(- - -)|g258>a|a270>g|c314>t,A105>V(+ - +)| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>c,A38>P(- - -)| a172>c,S58>R(- - -)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|a193>g,T65>A(- - -)| c234>g,I78>M(+ + -)|a254>g,N85>S(- - -)|g258>a|a270>g| c314>t,A105>V(+ - +)|g317>c,R106>T(- - -)|g319>t|a320>g| 316 (320) 221 (225) 207 (208) 14 (17) 2 (4) 12 (13) 3 (4) 3 4 0 1 0 0 0 2 (3) 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 0 3 0 0 0 0 0 2 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 2 0 0 1 0 0 0 0 0 0 0 117 114 110 4 2 2 2 1 0 0 0 0 0 0 0 1 0 0 4 (8) 4 3 (4) 1 (4) 0 (2) 1 (2) 0 (1) 0 1 0 0 0 0 0 0 (1) 0 0 (1) 0 aa,26-27(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|aa,152-153(FR2)|ta,154-155(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,154-155(FR2)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|tt,198-199(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|agct,162-165(FR2)|agta,169-172(CDR2)|ggta,187-190(CDR2)|ggca,220-223(FR3)|ggca,274-277(FR3)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|agct,162-165(FR2)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcattttcagtacttatcct atgcactgggtccgccaggctccagggaagggactggaatatgtttcagct attagtcgtaatggggataacgca tattatgcagactctgtgaagggcagattcaccatgtccagagacaattccaagagcacactgtatcttcagatgggcagcctgagagctgaggacatggctgtgtattactgt gtgacatgtggtgattacagctatgagtac VTCGDYSYEY IGHV3-64 IGHD3-22 IGHJ4 ggattcattttcagtacttatcct atgcactgggtccgccaggctccagggaagggactggaatatgtttcagct attagtcgtaatggggataacgca tattatgcagactctgtgaagggcagattcaccatgtccagagacaattccaagagcacactgtatcttcagatgggcagcctgagagctgaggacatggctgtgtattactgt gtgacatgtggtgattacagctatgagtac IGA1 13 213 9 4 6 9 3 4 2 0 2 9 JY8QFUQ01A5V9M 20796 productive Homsap IGHV3-48*02 F 864 88.94 193/217 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD6-6*01 F 1 8 8 20 8.8.20 [1.17.38.11] CARDDSRLWSSSFNHYFAMDVW in-frame + ctctggattcacctttagtgattatagaatgaattgggtccgccaggctccagggatggggctggaatgggttgcacacattagtaccagtggtagtagtatatactatgcagactctgtgaagggccgattcaccgtctccagagacgatgccaagaattcactttttctgcaaatggacagcctgagagacgacgacacggctgtatattactgtgcgagagacgattcacggctgtggagctcgtcatttaaccactatttcgctatggacgtctggggccaagggaccaccgtcaccgtctcctcagcatcccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccc NA IGA1 100 95 [309] c90>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|c114>a,S38>R(- - -)|c120>t|a143>t,K48>M(- + -)|g153>a|t160>g,S54>A(- + -)|t163>c,Y55>H(+ - -)|g173>c,S58>T(+ - +)|t174>c,S58>T(+ - +)|a184>g,S62>G(+ + -)|c191>g,T64>S(+ - +)|c192>t,T64>S(+ - +)|c201>t|a232>g,I78>V(+ - +)|a244>g,N82>D(+ + -)|c255>t|g261>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|g291>c,E97>D(+ - +)|g303>a| c90>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|c114>a,S38>R(- - -)| c120>t|a143>t,K48>M(- + -)|g153>a|t160>g,S54>A(- + -)|t163>c,Y55>H(+ - -)| g173>c,S58>T(+ - +)|t174>c,S58>T(+ - +)|a184>g,S62>G(+ + -)|c191>g,T64>S(+ - +)|c192>t,T64>S(+ - +)| c201>t|a232>g,I78>V(+ - +)|a244>g,N82>D(+ + -)|c255>t|g261>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|g291>c,E97>D(+ - +)|g303>a| 320 225 201 24 7 17 5 3 6 2 0 1 2 0 2 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 1 1 2 0 0 1 0 0 0 0 0 0 51 51 46 5 2 3 0 1 1 1 0 0 1 0 0 0 0 1 30 24 19 5 0 5 1 0 1 1 0 0 0 0 1 1 0 0 117 114 105 9 4 5 3 1 2 0 0 0 1 0 1 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtgattataga atgaattgggtccgccaggctccagggatggggctggaatgggttgcacac attagtaccagtggtagtagtata tactatgcagactctgtgaagggccgattcaccgtctccagagacgatgccaagaattcactttttctgcaaatggacagcctgagagacgacgacacggctgtatattactgt gcgagagacgattcacggctgtggagctcgtcatttaaccactatttcgctatggacgtc ARDDSRLWSSSFNHYFAMDV IGHV3-48 IGHD6-6 IGHJ6 ggattcacctttagtgattataga atgaattgggtccgccaggctccagggatggggctggaatgggttgcacac attagtaccagtggtagtagtata tactatgcagactctgtgaagggccgattcaccgtctccagagacgatgccaagaattcactttttctgcaaatggacagcctgagagacgacgacacggctgtatattactgt gcgagagacgattcacggctgtggagctcgtcatttaaccactatttcgctatggacgtc IGA1 24 213 16 8 9 14 7 10 6 1 8 9 JY8QFUQ01A5VSB 2688 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 936 92.63 201/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-10*01 F 2 8 8 13 8.8.13 [1.17.38.11] CANWLSGTHSKFDYW in-frame + ctctggattcaccttcagcaagtatgccatgagctgggtccgccaggctccaggggaggggctgcagtgggtctcagcaattagtggaaatggtgctgatatatactacgcagactccgtgaacggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgtgcgaattggctttcggggacacattctaagtttgactactggggcaagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] t90>c|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a142>g,K48>E(+ - -)|g151>c,E51>Q(+ + -)|t165>a|t174>a|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c192>t,S64>D(- - -)|c194>t,T65>I(- - -)|g216>c,K72>N(+ - -)|g261>a|t306>c| t90>c|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)| a142>g,K48>E(+ - -)|g151>c,E51>Q(+ + -)|t165>a| t174>a|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c192>t,S64>D(- - -)|c194>t,T65>I(- - -)| g216>c,K72>N(+ - -)|g261>a|t306>c| a318>t,K106>N(+ - -)|g319>t|a320>g| 317 (320) 222 (225) 206 16 (19) 5 (7) 11 (12) 2 (3) 4 2 2 0 0 0 (1) 2 3 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 0 1 0 0 0 0 0 1 0 0 51 51 48 3 1 2 1 0 0 0 0 0 0 1 1 0 0 0 30 24 17 7 1 6 1 2 2 0 0 0 0 1 1 0 0 0 117 114 111 3 2 1 0 1 0 1 0 0 0 0 1 0 0 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagcaagtatgcc atgagctgggtccgccaggctccaggggaggggctgcagtgggtctcagca attagtggaaatggtgctgatata tactacgcagactccgtgaacggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgt gcgaattggctttcggggacacattctaagtttgactac ANWLSGTHSKFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcaccttcagcaagtatgcc atgagctgggtccgccaggctccaggggaggggctgcagtgggtctcagca attagtggaaatggtgctgatata tactacgcagactccgtgaacggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgt gcgaattggctttcggggacacattctaagtttgactac IGA1 16 213 10 6 6 10 4 6 3 2 3 8 JY8QFUQ01A5WKX 19055 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 892 89.95 197/219 nt NA Homsap IGHJ3*01 F 178 84 42/50 nt Homsap IGHD6-19*01 F 1 8 8 16 8.8.16 [2.17.38.11] CAKEQRGSGWSRSALDVW in-frame - gcctctggattcacgtttggcagccacgccatgagctgggtccgccaggctccagggaaggggctggagtacgtctcaattgttactggtagcggacgcagcacatactacgcagagtctgtgaagggccggttcaccgtctccagagacaattccaaggacaccctgtatctgcaaatggacagcctgagagccgaggacacggccgtgtattattgtgcgaaagaacaacgaggcagtggctggtcccgatcggctcttgatgtctggggccaaggggcatgggtcaccgtctcttcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [299] c87>g|a103>g,S35>G(+ + -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|g163>a,A55>I(+ - +)|c164>t,A55>I(+ - +)|a166>g,I56>V(+ - +)|g170>c,S57>T(+ - +)|t177>c|t186>a|g187>c,G63>R(- - -)|t189>c,G63>R(- - -)|c207>g,D69>E(+ - +)|c210>t|a232>g,I78>V(+ - +)|a253>g,N85>D(+ + -)|g258>c|a274>g,N92>D(+ + -)|a303>g|c309>t| c87>g|a103>g,S35>G(+ + -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)| g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|g163>a,A55>I(+ - +)|c164>t,A55>I(+ - +)| a166>g,I56>V(+ - +)|g170>c,S57>T(+ - +)|t177>c|t186>a|g187>c,G63>R(- - -)|t189>c,G63>R(- - -)| c207>g,D69>E(+ - +)|c210>t|a232>g,I78>V(+ - +)|a253>g,N85>D(+ + -)|g258>c|a274>g,N92>D(+ + -)|a303>g|c309>t| 320 227 205 22 7 15 6 2 3 4 0 0 0 1 4 2 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 0 2 0 0 0 0 0 1 0 0 51 51 47 4 0 4 0 2 1 0 0 0 0 0 1 0 0 0 30 24 18 6 2 4 1 0 0 2 0 0 0 1 2 0 0 0 117 114 106 8 4 4 4 0 2 0 0 0 0 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacgtttggcagccacgcc atgagctgggtccgccaggctccagggaaggggctggagtacgtctcaatt gttactggtagcggacgcagcaca tactacgcagagtctgtgaagggccggttcaccgtctccagagacaattccaaggacaccctgtatctgcaaatggacagcctgagagccgaggacacggccgtgtattattgt gcgaaagaacaacgaggcagtggctggtcccgatcggctcttgatgtc AKEQRGSGWSRSALDV IGHV3-23 IGHD6-19 IGHJ3 ggattcacgtttggcagccacgcc atgagctgggtccgccaggctccagggaaggggctggagtacgtctcaatt gttactggtagcggacgcagcaca tactacgcagagtctgtgaagggccggttcaccgtctccagagacaattccaaggacaccctgtatctgcaaatggacagcctgagagccgaggacacggccgtgtattattgt gcgaaagaacaacgaggcagtggctggtcccgatcggctcttgatgtc IGG1 22 213 15 7 5 11 10 11 4 3 8 7 JY8QFUQ01A5XDS 18308 productive (see comment) Homsap IGHV1-2*02 F 939 93.46 200/214 nt 92.06 197/214 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-19*01 F 1 8 8 12 8.8.12 [0.17.38.11] CARGPVSGWYTDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'T' (1 nucleotides at position 18 to 18 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 44 of V-REGION: 'G' (1 nucleotides at position 43 to 43 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 57 of V-REGION: 'AACCCC' (6 nucleotides at position 82 to 87 in the user submitted sequence), (do not cause frameshift); tggatacaccttcaccgTgctactatctattctgggtgcgacGgggcccctggacaagggcttgagtggatgggatggatcAACCCCaaccctaagagtggtgacacacactatgcaccgaaattccagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggaactgaataggctgagatctgacgacacggccgtgtattactgtgcgagaggccctgtcagtggctggtacactgactactggggccagggaaccctggtcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [289] a115>c,M39>L(+ + -)|g117>a,M39>L(+ + -)|c118>t,H40>F(- - -)|a119>t,H40>F(- - -)|a131>g,Q44>R(+ - -)|c177>g,N59>K(+ - -)|g191>a,G64>D(- - -)|a196>c,N66>H(- - -)|a206>c,Q69>P(- - -)|g210>a|t213>c|g270>a|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)| a115>c,M39>L(+ + -)|g117>a,M39>L(+ + -)|c118>t,H40>F(- - -)|a119>t,H40>F(- - -)|a131>g,Q44>R(+ - -)| c177>g,N59>K(+ - -)|g191>a,G64>D(- - -)| a196>c,N66>H(- - -)|a206>c,Q69>P(- - -)|g210>a|t213>c|g270>a|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)| a320>g| 319 (320) 221 (222) 207 14 (15) 3 (4) 11 1 (2) 5 2 1 3 0 1 0 0 1 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 46 5 0 5 1 1 1 0 1 0 1 0 0 0 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 0 1 0 0 117 114 107 7 3 4 0 3 1 1 2 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatacaccttcaccggctactat ctattctgggtgcgacgggcccctggacaagggcttgagtggatgggatgg atcaaccctaagagtggtgacaca cactatgcaccgaaattccagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggaactgaataggctgagatctgacgacacggccgtgtattactgt gcgagaggccctgtcagtggctggtacactgactac ARGPVSGWYTDY IGHV1-2 IGHD6-19 IGHJ4 ggatacaccttcaccggctactat ctattctgggtgcgacgggcccctggacaagggcttgagtggatgggatgg atcaaccctaagagtggtgacaca cactatgcaccgaaattccagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggaactgaataggctgagatctgacgacacggccgtgtattactgt gcgagaggccctgtcagtggctggtacactgactac IGA1 14 213 9 5 7 8 2 6 3 0 9 2 JY8QFUQ01A5ZKW 3960 productive (see comment) Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F or Homsap IGHV3-15*06 F 930 91.03 203/223 nt 90.58 202/223 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD1-26*01 F 2 8 10 14 8.10.14 [1.17.38.11] CTTRPRGWEPLGLDYW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 72 of V-REGION: 'A' (1 nucleotides at position 128 to 128 in the user submitted sequence), (cause frameshift); ctctggattcactttcagtgacgcctggatggtctgggtccgccaggctccagggaaggggctggagtgggttggccttattaagaccaaacctgatggttggacaacaatctactctgcacccgtgAaaaggcagattcatcatttcaagagatgattcaaaaaacatggtgtttctgcaaatgaacaacctgagaaccgaggacacagccctttattactgtaccacacggccccgggggtgggaaccacttggccttgactactggggcctgggaaccctggtcaccgtctcctcagcatccccggaccagccccaaaggtcttccccgctgagcctcgacagcacccccccaaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 71 74 [302, 300] a106>g,N36>D(+ + -)|a118>g,S40>V(- - -)|g119>t,S40>V(- - -)|g164>t,R55>L(- + -)|a171>g|g173>c,S58>T(+ - +)|a178>c,T60>P(+ + -)|g187>t,G63>W(- - -)|g196>a,D66>I(- - -)|a197>t,D66>I(- - -)|g202>t,A68>S(- + -)|c230>t,T77>I(- - -)|c234>t|c257>t,T86>M(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|g278>a,S93>N(- - -)|a284>g,K95>R(+ + +)|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)| a106>g,N36>D(+ + -)| a118>g,S40>V(- - -)|g119>t,S40>V(- - -)|g164>t,R55>L(- + -)| a171>g|g173>c,S58>T(+ - +)|a178>c,T60>P(+ + -)|g187>t,G63>W(- - -)| g196>a,D66>I(- - -)|a197>t,D66>I(- - -)|g202>t,A68>S(- + -)|c230>t,T77>I(- - -)|c234>t|c257>t,T86>M(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|g278>a,S93>N(- - -)|a284>g,K95>R(+ + +)|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)| g319>c|a320>g| 318 (320) 229 (231) 209 20 (22) 2 (4) 18 4 (5) 2 3 0 1 0 2 0 2 (3) 1 5 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 51 48 3 0 3 1 0 0 0 0 0 0 0 0 0 2 0 30 30 26 4 1 3 1 0 0 0 1 0 0 0 1 0 1 0 117 114 102 12 1 11 1 2 3 0 0 0 2 0 1 1 2 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| ctct ggattcactttcagtgacgcctgg atggtctgggtccgccaggctccagggaaggggctggagtgggttggcctt attaagaccaaacctgatggttggacaaca atctactctgcacccgtgaaaggcagattcatcatttcaagagatgattcaaaaaacatggtgtttctgcaaatgaacaacctgagaaccgaggacacagccctttattactgt accacacggccccgggggtgggaaccacttggccttgactac TTRPRGWEPLGLDY IGHV3-15 IGHD1-26 IGHJ4 ggattcactttcagtgacgcctgg atggtctgggtccgccaggctccagggaaggggctggagtgggttggcctt attaagaccaaacctgatggttggacaaca atctactctgcacccgtgaaaggcagattcatcatttcaagagatgattcaaaaaacatggtgtttctgcaaatgaacaacctgagaaccgaggacacagccctttattactgt accacacggccccgggggtgggaaccacttggccttgactac IGA2 20 219 9 11 5 13 4 7 1 1 14 4 JY8QFUQ01A60V7 12142 productive Homsap IGHV3-48*01 F 918 91.71 199/217 nt NA Homsap IGHJ6*02 F 229 85.48 53/62 nt Homsap IGHD3-9*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARQTGNYYYFGMDVW in-frame + ctctggattcctcttcagtagctttaacatgaactgggtccgccaggttccagggaagggtctggagtgggtttcatacattaatagtagaggtactaacatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaggaattcactgtatctgcaaatgaacagcctgagagccgacgacacggccatctactactgtgcgagacaaactgggaattattattacttcggtatggacgtctggggccaagggactacggtcaccgtctcctctgcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [290] a85>c,T29>L(- - -)|c86>t,T29>L(- - -)|a110>t,Y37>F(- + -)|g113>a,S38>N(- - -)|c134>t,A45>V(+ - +)|g147>t|g170>a,S57>N(- - -)|t177>a,S59>R(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|c191>a,T64>N(- + -)|a251>g,K84>R(+ + +)|c255>t|g291>c,E97>D(+ - +)|t300>c|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)|t306>c| a85>c,T29>L(- - -)|c86>t,T29>L(- - -)|a110>t,Y37>F(- + -)|g113>a,S38>N(- - -)| c134>t,A45>V(+ - +)|g147>t| g170>a,S57>N(- - -)|t177>a,S59>R(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|c191>a,T64>N(- + -)| a251>g,K84>R(+ + +)|c255>t|g291>c,E97>D(+ - +)|t300>c|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)|t306>c| g319>c| 318 (320) 223 (225) 205 (206) 18 (19) 4 (5) 14 2 3 3 2 1 1 1 1 3 (4) 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 1 0 1 0 1 0 0 0 0 0 51 51 49 2 1 1 0 0 1 0 0 0 0 0 0 0 1 0 30 24 19 5 0 5 1 1 0 0 0 1 0 1 1 0 0 0 117 114 107 7 3 4 1 1 1 2 0 0 0 0 2 0 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcctcttcagtagctttaac atgaactgggtccgccaggttccagggaagggtctggagtgggtttcatac attaatagtagaggtactaacata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaggaattcactgtatctgcaaatgaacagcctgagagccgacgacacggccatctactactgt gcgagacaaactgggaattattattacttcggtatggacgtc ARQTGNYYYFGMDV IGHV3-48 IGHD3-9 IGHJ6 ggattcctcttcagtagctttaac atgaactgggtccgccaggttccagggaagggtctggagtgggtttcatac attaatagtagaggtactaacata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaggaattcactgtatctgcaaatgaacagcctgagagccgacgacacggccatctactactgt gcgagacaaactgggaattattattacttcggtatggacgtc IGA1 18 213 10 8 6 11 4 7 4 0 5 9 JY8QFUQ01A64VB 9118 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 929 91.4 202/221 nt NA Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD3-3*01 F 2 10 7 11 10.7.11 [1.17.38.11] CARSYGFSRFDPW in-frame + tctctggtggctccatcaccagtagtctttaccgctggggctggatccgccagtccccagggaagggcctggagtggatagggaatatcttttatggtgggaccacctactacaacccgtccctcaagagtcgagtcaccttatccatagacacgtccaagagccagttctccctgaagctgtcctctgtgaccgccgcagacacggctctatattactgtgcgagatcgtacggtttctctcggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [285] g92>c,S31>T(+ - +)|a106>c,S36>L(- - -)|g107>t,S36>L(- - -)|t112>c,Y38>R(- - -)|a113>g,Y38>R(- - -)|c133>t,P45>S(+ - -)|g147>c|t159>a|g164>a,S55>N(- - -)|a170>t,Y57>F(- + -)|a175>g,S59>G(+ + -)|g191>c,S64>T(+ - +)|a232>t,I78>L(+ + +)|g238>a,V80>I(+ - +)|a254>g,N85>S(- - -)|a274>t|g275>c|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| g92>c,S31>T(+ - +)|a106>c,S36>L(- - -)|g107>t,S36>L(- - -)|t112>c,Y38>R(- - -)|a113>g,Y38>R(- - -)| c133>t,P45>S(+ - -)|g147>c|t159>a|g164>a,S55>N(- - -)| a170>t,Y57>F(- + -)|a175>g,S59>G(+ + -)|g191>c,S64>T(+ - +)| a232>t,I78>L(+ + +)|g238>a,V80>I(+ - +)|a254>g,N85>S(- - -)|a274>t|g275>c|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| c319>t|a320>c| 318 (320) 227 (229) 208 19 (21) 4 (6) 15 3 3 1 (2) 1 1 (2) 0 3 1 5 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 25 5 0 5 1 0 0 1 1 0 0 0 1 0 1 0 51 51 47 4 2 2 0 1 1 0 0 0 0 1 1 0 0 0 30 21 18 3 0 3 1 0 0 0 0 0 1 0 1 0 0 0 117 114 107 7 2 5 1 2 0 0 0 0 2 0 2 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 (1) 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcaccagtagtctttaccgc tggggctggatccgccagtccccagggaagggcctggagtggatagggaat atcttttatggtgggaccacc tactacaacccgtccctcaagagtcgagtcaccttatccatagacacgtccaagagccagttctccctgaagctgtcctctgtgaccgccgcagacacggctctatattactgt gcgagatcgtacggtttctctcggttcgacccc ARSYGFSRFDP IGHV4-39 IGHD3-3 IGHJ5 ggtggctccatcaccagtagtctttaccgc tggggctggatccgccagtccccagggaagggcctggagtggatagggaat atcttttatggtgggaccacc tactacaacccgtccctcaagagtcgagtcaccttatccatagacacgtccaagagccagttctccctgaagctgtcctctgtgaccgccgcagacacggctctatattactgt gcgagatcgtacggtttctctcggttcgacccc IGA1 19 216 8 11 4 10 4 9 4 0 7 8 JY8QFUQ01A666E 14021 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 982 94.52 207/219 nt NA Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 196 88 44/50 nt Homsap IGHD2-15*01 F 3 8 8 17 8.8.17 [2.17.38.11] CARDTLEVVSNMDDAFDLW in-frame - gcctctggattcacctttagtcgttatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatacattagtaagactactaatgacatatactatgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtatttctgtgcgagagatactctagaggtggtgtcgaatatggatgatgcttttgatttgtggggccaagggacagtggtcaatgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [301] c90>t|a106>c,S36>R(- - -)|c108>t,S36>R(- - -)|c164>a,S55>Y(+ - -)|g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g176>c,S59>T(+ - +)|g185>c,S62>T(+ - +)|g188>a,S63>N(- - -)|t190>g,Y64>D(- - -)|c201>t|a308>t,Y103>F(- + -)| c90>t|a106>c,S36>R(- - -)|c108>t,S36>R(- - -)| c164>a,S55>Y(+ - -)| g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g176>c,S59>T(+ - +)|g185>c,S62>T(+ - +)|g188>a,S63>N(- - -)|t190>g,Y64>D(- - -)| c201>t|a308>t,Y103>F(- + -)| 320 227 215 12 2 10 0 2 3 0 1 1 1 0 2 0 0 2 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 2 0 1 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 30 24 18 6 0 6 0 2 0 0 0 0 0 0 2 0 0 2 117 114 112 2 1 1 0 0 1 0 0 0 1 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacctttagtcgttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatac attagtaagactactaatgacata tactatgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtatttctgt gcgagagatactctagaggtggtgtcgaatatggatgatgcttttgatttg ARDTLEVVSNMDDAFDL IGHV3-21 IGHD2-15 IGHJ3 ggattcacctttagtcgttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatac attagtaagactactaatgacata tactatgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtatttctgt gcgagagatactctagaggtggtgtcgaatatggatgatgcttttgatttg IGA1 12 213 5 7 5 8 0 4 1 1 2 8 JY8QFUQ01A68J9 17403 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 1030 97.69 211/216 nt 97.22 210/216 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD6-25*01 F 1 8 8 13 8.8.13 [1.16.38.11] CARDLTGYSSERNYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 58 in the user submitted sequence), (cause frameshift) ctctggattcaccttcagtaactatagcatgaactgggtccgccaggctccagggaagggctggagtgggtctcatccattagtagtagtgctaggtacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgcgagacctgaccgggtatagcagcgaacgaaactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [286] g107>a,S36>N(- - -)|a184>g,S62>A(- + -)|g185>c,S62>A(- + -)|t189>g,S63>R(- - -)|a263>c,Y88>S(+ - -)|a316>c| g107>a,S36>N(- - -)| a184>g,S62>A(- + -)|g185>c,S62>A(- + -)|t189>g,S63>R(- - -)| a263>c,Y88>S(+ - -)| a316>c| 320 224 218 6 1 5 1 1 0 0 2 0 0 0 1 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 50 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 1 0 0 1 117 114 113 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 8 8 7 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtaactatagc atgaactgggtccgccaggctccagggaagggctggagtgggtctcatcc attagtagtagtgctaggtacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgcgagacctgaccgggtatagcagcgaacgaaactac ARDLTGYSSERNY IGHV3-21 IGHD6-25 IGHJ4 ggattcaccttcagtaactatagc atgaactgggtccgccaggctccagggaagggctggagtgggtctcatcc attagtagtagtgctaggtacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgcgagacctgaccgggtatagcagcgaacgaaactac IGA2 5 212 2 3 1 2 1 3 0 0 1 4 JY8QFUQ01A698F 658 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 945 93.09 202/217 nt NA Homsap IGHJ3*02 F 232 96 48/50 nt Homsap IGHD3-22*01 F 2 8 8 19 8.8.19 [1.17.38.11] CAKDREDSSGYYFFRDGFDIW in-frame + ctctggattcacgtttaggacctatgccatgacctgggtccgccaggctccagggaaggggctggagtgggtctccggtattggtggtagtggtcgaaccacacactacgcagactccgtgcagggccggttcaccatctccagagacaattccaagaacacggtggatctgcaaatgaacagcctgagagccgaggacacggccatatattactgtgcgaaagatcgtgaagatagtagtggttattacttcttccgtgatggttttgatatttggggccaagggacaatggtcaccgtctcttcagcatcccctgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttc NA IGA1 100 89 [306] c87>g|c105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|g119>c,S40>T(+ - +)|a162>c|c164>g,A55>G(- + -)|a169>g,S57>G(+ + -)|g187>c,G63>R(- - -)|t189>a,G63>R(- - -)|g191>c,S64>T(+ - +)|t196>c,Y66>H(+ - -)|a214>c,K72>Q(+ - -)|c259>g,L87>V(+ - +)|t262>g,Y88>D(- - -)|g301>a,V101>I(+ - +)| c87>g|c105>g,S35>R(- - -)|g107>c,S36>T(+ - +)| g119>c,S40>T(+ - +)|a162>c|c164>g,A55>G(- + -)| a169>g,S57>G(+ + -)|g187>c,G63>R(- - -)|t189>a,G63>R(- - -)|g191>c,S64>T(+ - +)| t196>c,Y66>H(+ - -)|a214>c,K72>Q(+ - -)|c259>g,L87>V(+ - +)|t262>g,Y88>D(- - -)|g301>a,V101>I(+ - +)| 320 225 210 15 2 13 1 1 0 1 2 0 0 1 4 4 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 0 0 0 0 0 0 1 2 0 0 51 51 48 3 1 2 0 0 0 0 1 0 0 0 1 1 0 0 30 24 20 4 0 4 1 0 0 0 0 0 0 1 2 0 0 0 117 114 109 5 0 5 0 1 0 1 1 0 0 0 0 1 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgtttaggacctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctccggt attggtggtagtggtcgaaccaca cactacgcagactccgtgcagggccggttcaccatctccagagacaattccaagaacacggtggatctgcaaatgaacagcctgagagccgaggacacggccatatattactgt gcgaaagatcgtgaagatagtagtggttattacttcttccgtgatggttttgatatt AKDREDSSGYYFFRDGFDI IGHV3-23 IGHD3-22 IGHJ3 ggattcacgtttaggacctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctccggt attggtggtagtggtcgaaccaca cactacgcagactccgtgcagggccggttcaccatctccagagacaattccaagaacacggtggatctgcaaatgaacagcctgagagccgaggacacggccatatattactgt gcgaaagatcgtgaagatagtagtggttattacttcttccgtgatggttttgatatt IGA1 15 213 3 12 1 9 2 6 1 1 7 6 JY8QFUQ01A699S 12396 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 905 90.83 198/218 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-21*02 F 3 8 8 16 8.8.16 [1.17.38.11] CAKDRYPRSTAITAPDYW in-frame + cctctggattcccctttagcaactatgccatgacctgggtccgccaggctccaggggagggactggaatgggtctcaagtattagaggtagtggtgacaggacatcctacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacacggtgtatctgcagctgaacaacctgagagccgaggacagggccgaatattactgtgcgaaagatcgctaccctcggtcgacagctattacagcgcctgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggctcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [298, 297] a85>c,T29>P(+ + -)|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|a142>g,K48>E(+ - -)|g147>a|g153>a|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t171>a,S57>R(- - -)|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|c192>g,S64>R(- - -)|a197>c,Y66>S(+ - -)|a215>g,K72>R(+ + +)|c259>g,L87>V(+ - +)|a270>g|a271>c,M91>L(+ + -)|g278>a,S93>N(- - -)|c296>g,T99>R(- - -)|t302>a,V101>E(- + -)| a85>c,T29>P(+ + -)|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|a142>g,K48>E(+ - -)|g147>a|g153>a|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| t171>a,S57>R(- - -)|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|c192>g,S64>R(- - -)| a197>c,Y66>S(+ - -)|a215>g,K72>R(+ + +)|c259>g,L87>V(+ - +)|a270>g|a271>c,M91>L(+ + -)|g278>a,S93>N(- - -)|c296>g,T99>R(- - -)|t302>a,V101>E(- + -)| 320 226 206 20 3 17 3 6 0 1 3 0 0 2 1 4 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 1 0 0 0 0 0 0 0 51 51 45 6 2 4 1 3 0 0 0 0 0 0 1 1 0 0 30 24 20 4 0 4 0 1 0 1 0 0 0 1 0 1 0 0 117 114 106 8 1 7 2 1 0 0 2 0 0 1 0 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcccctttagcaactatgcc atgacctgggtccgccaggctccaggggagggactggaatgggtctcaagt attagaggtagtggtgacaggaca tcctacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacacggtgtatctgcagctgaacaacctgagagccgaggacagggccgaatattactgt gcgaaagatcgctaccctcggtcgacagctattacagcgcctgactac AKDRYPRSTAITAPDY IGHV3-23 IGHD2-21 IGHJ4 ggattcccctttagcaactatgcc atgacctgggtccgccaggctccaggggagggactggaatgggtctcaagt attagaggtagtggtgacaggaca tcctacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacacggtgtatctgcagctgaacaacctgagagccgaggacagggccgaatattactgt gcgaaagatcgctaccctcggtcgacagctattacagcgcctgactac IGA1 20 213 10 10 6 11 4 9 3 0 11 6 JY8QFUQ01A69S0 13964 productive Homsap IGHV3-48*01 F 1075 99.54 215/216 nt NA Homsap IGHJ6*02 F 256 90.32 56/62 nt Homsap IGHD3-3*01 F 3 8 8 18 8.8.18 [1.17.38.11] CASIFGVVPLGNYYYGMDVW in-frame - tctggattcaccttcagtagctatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagtagtagtagtagtaccatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgcgcctcgatttttggagtggttcccctggggaactactactacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [302] t312>c| t312>c| g315>c|a316>t,R106>S(- - -)|g317>c,R106>S(- - -)|a318>g,R106>S(- - -)|g319>a|a320>t| 314 (320) 218 (224) 217 1 (7) 1 (4) 0 (3) 0 (1) 0 (1) 0 1 0 0 0 (2) 0 0 (2) 0 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 113 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 2 (8) 2 2 0 (6) 0 (3) 0 (3) 0 (1) 0 (1) 0 0 0 0 0 (2) 0 0 (2) 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtagtagtagtagtaccata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgc gcctcgatttttggagtggttcccctggggaactactactacggtatggacgtc ASIFGVVPLGNYYYGMDV IGHV3-48 IGHD3-3 IGHJ6 ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtagtagtagtagtaccata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgc gcctcgatttttggagtggttcccctggggaactactactacggtatggacgtc IGG1 1 213 1 0 0 0 1 1 1 0 0 0 JY8QFUQ01A6BXT 20262 productive Homsap IGHV3-9*01 F 896 90.37 197/218 nt NA Homsap IGHJ2*01 F 247 96.23 51/53 nt Homsap IGHD3-22*01 F 2 8 8 22 8.8.22 [1.17.38.11] CVRQAYYHDSSAYFFSRYWYFDLW in-frame + cctctggattcacttttgatgactctgccatgcactgggtgcggcaagctccagggaagggcccggagtgggtcgcaggtattagtggaaatagtggaaatataggatatgcggactcagtgaagggccgatgcaccatctccagagacaacgccaagaagtccctgtttctgcaaattaaaagtctgagagttgaggacacggccttatattattgtgtgaggcaagcgtattaccatgatagcagtgcttacttcttctcccgctactggtacttcgatctctggggccgtggcaccccggtcactgtctcttcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [316] c87>t|t108>c|a110>c,Y37>S(+ - -)|c126>g|t149>c,L50>P(- - -)|t160>g,S54>A(- + -)|t172>g,W58>G(- - -)|g174>a,W58>G(- - -)|t189>a|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)|c198>a|t210>a|t227>g,F76>C(+ - -)|c255>g,N85>K(+ - -)|a263>t,Y88>F(- + -)|g273>t,M91>I(+ + -)|c276>a,N92>K(+ - -)|c287>t,A96>V(+ - +)|g303>a|c309>t| c87>t|t108>c|a110>c,Y37>S(+ - -)| c126>g|t149>c,L50>P(- - -)|t160>g,S54>A(- + -)| t172>g,W58>G(- - -)|g174>a,W58>G(- - -)|t189>a|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)| c198>a|t210>a|t227>g,F76>C(+ - -)|c255>g,N85>K(+ - -)|a263>t,Y88>F(- + -)|g273>t,M91>I(+ + -)|c276>a,N92>K(+ - -)|c287>t,A96>V(+ - +)|g303>a|c309>t| c314>t,A105>V(+ - +)|a315>g,A105>V(+ - +)|a317>g,K106>R(+ + +)|a318>g,K106>R(+ + +)|g319>c,D107>Q(+ - -)|t321>a,D107>Q(+ - -)|a322>g| 313 (322) 219 (228) 198 (200) 21 (28) 8 (9) 13 (19) 0 (4) 3 4 (5) 2 1 2 1 2 (3) 0 (1) 2 1 3 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 1 1 1 0 0 0 0 0 0 0 51 51 48 3 1 2 0 0 0 1 0 0 0 0 0 1 0 1 30 24 19 5 1 4 0 2 1 0 0 0 0 1 0 0 0 1 117 114 104 10 4 6 0 1 2 0 0 2 1 1 0 1 1 1 1 (10) 1 1 (3) 0 (7) 0 (1) 0 (6) 0 (4) 0 0 (1) 0 0 0 0 0 (1) 0 (1) 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacttttgatgactctgcc atgcactgggtgcggcaagctccagggaagggcccggagtgggtcgcaggt attagtggaaatagtggaaatata ggatatgcggactcagtgaagggccgatgcaccatctccagagacaacgccaagaagtccctgtttctgcaaattaaaagtctgagagttgaggacacggccttatattattgt gtgaggcaagcgtattaccatgatagcagtgcttacttcttctcccgctactggtacttcgatctc VRQAYYHDSSAYFFSRYWYFDL IGHV3-9 IGHD3-22 IGHJ2 ggattcacttttgatgactctgcc atgcactgggtgcggcaagctccagggaagggcccggagtgggtcgcaggt attagtggaaatagtggaaatata ggatatgcggactcagtgaagggccgatgcaccatctccagagacaacgccaagaagtccctgtttctgcaaattaaaagtctgagagttgaggacacggccttatattattgt gtgaggcaagcgtattaccatgatagcagtgcttacttcttctcccgctactggtacttcgatctc IGG2 21 213 9 12 7 12 2 9 5 3 8 5 JY8QFUQ01A6CNG 12872 productive Homsap IGHV4-61*01 F (see comment) 767 83.26 184/221 nt NA Homsap IGHJ3*02 F (see comment) 178 84 42/50 nt Homsap IGHD3-22*01 F 2 10 7 13 10.7.13 [1.17.38.11] CARDGNRGYYSFETW in-frame + Low V-REGION identity (83,26% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*01 and Homsap_IGHJ4*03 and Homsap_IGHJ5*01 (highest number of consecutive identical nucleotides) tgtctggtggctccgtcagcagtggaaattcctactggacttggatccgccagtcccccgagaagggactggagtggcttgcatatattcgaaacactgggacaaccaactacaacccctccctcaagagtagactcaccatgtctctggacatgtctaggaatcagttctccctgaggctgaacgatgtgaccgctgcggacacggccatatattactgtgcgagagacggcaatagaggttattactcttttgaaacctggggccgagggacattggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [292] c75>g|t105>a|g107>a,S36>N(- - -)|a110>c,Y37>S(+ - -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g129>c|c133>t,P45>S(+ - -)|a138>c|g140>a,G47>E(- - -)|a157>c,I53>L(+ + +)|g161>c,G54>A(- + -)|g162>a,G54>A(- + -)|c168>t|t169>c,Y57>R(- - -)|a170>g,Y57>R(- - -)|t171>a,Y57>R(- - -)|t172>a,Y58>N(- - -)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)|c223>a|g226>c,V76>L(+ - +)|a234>g,I78>M(+ + -)|a237>t|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|c245>t,T82>M(- - -)|c249>t|a251>g,K84>R(+ + +)|c255>t|a269>g,K90>R(+ + +)|g275>a,S92>N(- - -)|t277>g,S93>D(- - -)|c278>a,S93>D(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| c75>g| t105>a|g107>a,S36>N(- - -)|a110>c,Y37>S(+ - -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g129>c|c133>t,P45>S(+ - -)|a138>c|g140>a,G47>E(- - -)|a157>c,I53>L(+ + +)|g161>c,G54>A(- + -)|g162>a,G54>A(- + -)| c168>t|t169>c,Y57>R(- - -)|a170>g,Y57>R(- - -)|t171>a,Y57>R(- - -)|t172>a,Y58>N(- - -)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)| c223>a|g226>c,V76>L(+ - +)|a234>g,I78>M(+ + -)|a237>t|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|c245>t,T82>M(- - -)|c249>t|a251>g,K84>R(+ + +)|c255>t|a269>g,K90>R(+ + +)|g275>a,S92>N(- - -)|t277>g,S93>D(- - -)|c278>a,S93>D(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 229 192 37 9 28 5 6 6 1 3 3 1 3 7 1 0 1 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 30 27 3 1 2 0 1 0 0 1 0 0 1 0 0 0 0 51 51 42 9 2 7 0 2 2 0 2 0 0 0 3 0 0 0 30 21 13 8 1 7 1 0 1 1 0 1 0 2 2 0 0 0 117 114 98 16 4 12 4 3 3 0 0 2 1 0 2 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tgtct ggtggctccgtcagcagtggaaattcctac tggacttggatccgccagtcccccgagaagggactggagtggcttgcatat attcgaaacactgggacaacc aactacaacccctccctcaagagtagactcaccatgtctctggacatgtctaggaatcagttctccctgaggctgaacgatgtgaccgctgcggacacggccatatattactgt gcgagagacggcaatagaggttattactcttttgaaacc ARDGNRGYYSFET IGHV4-61 IGHD3-22 IGHJ3 ggtggctccgtcagcagtggaaattcctac tggacttggatccgccagtcccccgagaagggactggagtggcttgcatat attcgaaacactgggacaacc aactacaacccctccctcaagagtagactcaccatgtctctggacatgtctaggaatcagttctccctgaggctgaacgatgtgaccgctgcggacacggccatatattactgt gcgagagacggcaatagaggttattactcttttgaaacc IGG1 36 216 18 18 12 22 6 14 6 2 19 9 JY8QFUQ01A6CSB 16167 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 909 91.24 198/217 nt NA Homsap IGHJ2*01 F 247 96.23 51/53 nt Homsap IGHD1-1*01 F 1 8 8 15 8.8.15 [1.17.38.11] CAKVSGNSRTYWYLDIW in-frame + ttctggatacaactttcccagatattggatcgcctgggtgcgccagattcccgggagaggcctggagtgggtggggatgatctatcctggtgactctgagaccagatacagtccgtccttccaaggccaggtcaccatctcagccgacgcgtccatgaacaccgcctacctgcagtggagcagcctgaaggcctccgacaccgccatatacttctgtgcgaaggtctctggtaactctcgaacctactggtacctcgatatctggggccgtggcaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [293] g86>a,S29>N(- - -)|a103>c,T35>P(+ + -)|c108>a,S36>R(- - -)|c111>t|g119>c,G40>A(- + -)|g135>t,M45>I(+ + -)|a143>g,K48>R(+ + +)|a157>g,M53>V(+ - -)|c165>g,I55>M(+ + -)|t192>g,D64>E(+ - +)|c204>t|a244>g,K82>A(- - -)|a245>c,K82>A(- - -)|c252>g,I84>M(+ + -)|g254>a,S85>N(- - -)|g291>c|g303>a,M101>I(+ + -)|t306>c|a308>t,Y103>F(- + -)| g86>a,S29>N(- - -)|a103>c,T35>P(+ + -)|c108>a,S36>R(- - -)|c111>t| g119>c,G40>A(- + -)|g135>t,M45>I(+ + -)|a143>g,K48>R(+ + +)|a157>g,M53>V(+ - -)|c165>g,I55>M(+ + -)| t192>g,D64>E(+ - +)| c204>t|a244>g,K82>A(- - -)|a245>c,K82>A(- - -)|c252>g,I84>M(+ + -)|g254>a,S85>N(- - -)|g291>c|g303>a,M101>I(+ + -)|t306>c|a308>t,Y103>F(- + -)| g317>a,R106>K(+ + +)|a318>g,R106>K(+ + +)|c319>g|a320>t| 316 (320) 221 (225) 202 19 (23) 4 (6) 15 (17) 3 (4) 3 (4) 2 1 2 1 1 (2) 0 2 2 (3) 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 1 0 1 1 0 0 0 0 0 0 51 51 46 5 0 5 2 0 0 0 0 0 0 0 1 1 1 0 30 24 23 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 117 114 105 9 3 6 1 2 1 1 1 0 1 0 1 1 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 (1) 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ttct ggatacaactttcccagatattgg atcgcctgggtgcgccagattcccgggagaggcctggagtgggtggggatg atctatcctggtgactctgagacc agatacagtccgtccttccaaggccaggtcaccatctcagccgacgcgtccatgaacaccgcctacctgcagtggagcagcctgaaggcctccgacaccgccatatacttctgt gcgaaggtctctggtaactctcgaacctactggtacctcgatatc AKVSGNSRTYWYLDI IGHV5-51 IGHD1-1 IGHJ2 ggatacaactttcccagatattgg atcgcctgggtgcgccagattcccgggagaggcctggagtgggtggggatg atctatcctggtgactctgagacc agatacagtccgtccttccaaggccaggtcaccatctcagccgacgcgtccatgaacaccgcctacctgcagtggagcagcctgaaggcctccgacaccgccatatacttctgt gcgaaggtctctggtaactctcgaacctactggtacctcgatatc IGA1 19 213 9 10 5 11 4 8 3 1 11 4 JY8QFUQ01A6HGP 2400 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F (see comment) 795 84.3 188/223 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD6-19*01 F 2 8 10 15 8.10.15 [1.17.38.11] CTTDSFHIAVHGVFDFW in-frame + Low V-REGION identity (84,30% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ttctgcattcatgttcaacaaagcctggatgaattgggtccgccaggctccaggaaagggactggagtgggttggccgtattaaaagtaacggcgacggtgcgacagtcgactacgctgcacccgtgaaaggccgattcaccatctcaagagatgattcacagaataccctcttcttacaaatgagcagcctgaaagccgaggacacagccgtctattattgtaccacagatagcttccatattgcagtgcatggcgtctttgacttctggggccagggagccctagtcaccgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctgtccaggcttcttccccca NA IGA1 100 100 [299] c75>t|g80>c,G27>A(- + -)|c86>t,T29>M(- - -)|t87>g,T29>M(- - -)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|c108>a,N36>K(+ - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g141>a|g147>a|c174>t|a177>c,K59>N(+ - -)|a178>g,T60>G(+ - -)|c179>g,T60>G(+ - -)|t180>c,T60>G(+ - -)|t183>c|g188>c,G63>A(- + -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|a195>c,T65>V(- - -)|a223>c|a250>c,K84>Q(+ - -)|a252>g,K84>Q(+ - -)|c255>t|g258>c|g261>c|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|a275>g,N92>S(- - -)|a286>g,T96>A(- - -)|g303>c|c309>t| c75>t| g80>c,G27>A(- + -)|c86>t,T29>M(- - -)|t87>g,T29>M(- - -)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|c108>a,N36>K(+ - -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g141>a|g147>a| c174>t|a177>c,K59>N(+ - -)|a178>g,T60>G(+ - -)|c179>g,T60>G(+ - -)|t180>c,T60>G(+ - -)|t183>c|g188>c,G63>A(- + -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|a195>c,T65>V(- - -)| a223>c|a250>c,K84>Q(+ - -)|a252>g,K84>Q(+ - -)|c255>t|g258>c|g261>c|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|a275>g,N92>S(- - -)|a286>g,T96>A(- - -)|g303>c|c309>t| 320 231 196 35 13 22 5 5 8 4 4 1 1 0 5 1 0 1 78 4 3 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 1 1 1 0 1 0 0 1 0 0 1 51 51 47 4 2 2 0 3 1 0 0 0 0 0 0 0 0 0 30 30 20 10 2 8 2 0 2 2 2 0 0 0 1 1 0 0 117 114 100 14 8 6 3 1 3 1 2 0 1 0 3 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| ttct gcattcatgttcaacaaagcctgg atgaattgggtccgccaggctccaggaaagggactggagtgggttggccgt attaaaagtaacggcgacggtgcgacagtc gactacgctgcacccgtgaaaggccgattcaccatctcaagagatgattcacagaataccctcttcttacaaatgagcagcctgaaagccgaggacacagccgtctattattgt accacagatagcttccatattgcagtgcatggcgtctttgacttc TTDSFHIAVHGVFDF IGHV3-15 IGHD6-19 IGHJ4 gcattcatgttcaacaaagcctgg atgaattgggtccgccaggctccaggaaagggactggagtgggttggccgt attaaaagtaacggcgacggtgcgacagtc gactacgctgcacccgtgaaaggccgattcaccatctcaagagatgattcacagaataccctcttcttacaaatgagcagcctgaaagccgaggacacagccgtctattattgt accacagatagcttccatattgcagtgcatggcgtctttgacttc IGA1 34 219 21 13 12 19 9 15 10 2 8 14 JY8QFUQ01A6IQL 19474 productive Homsap IGHV4-4*02 F, or Homsap IGHV4-4*03 F or Homsap IGHV4-4*04 F or Homsap IGHV4-4*05 F 986 94.95 207/218 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD2-21*02 F 3 9 7 13 9.7.13 [1.17.38.11] CARGEMTAEYYFDYW in-frame + tctctggtggctccatcagcagtgttaactggtggagttgggtccgccagcccccagggaaggggctggagtggattggggagatctctcacagtgggaacaccaactacagcccgtccctcaagggtcgagtcaccatatcaataaacaagtccaagaaccaattctccctgaagctgagctctgtgaccgccgcggacacggccgtgtattactgtgcgagaggggaaatgactgccgagtactactttgattactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [288] a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|a165>g|a170>c,Y57>S(+ - -)|t174>c|g191>a,S64>N(- - -)|a203>g,N68>S(- - -)|a220>g,S74>G(+ + -)|g238>a,V80>I(+ - +)|g241>a,D81>N(+ + -)|g258>a| a106>g,S36>V(- - -)|g107>t,S36>V(- - -)| a165>g| a170>c,Y57>S(+ - -)|t174>c|g191>a,S64>N(- - -)| a203>g,N68>S(- - -)|a220>g,S74>G(+ + -)|g238>a,V80>I(+ - +)|g241>a,D81>N(+ + -)|g258>a| a320>g| 319 (320) 225 (226) 214 11 (12) 3 (4) 8 4 (5) 4 0 1 1 0 0 0 0 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 27 25 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 51 50 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 30 21 18 3 1 2 0 1 0 1 1 0 0 0 0 0 0 0 117 114 109 5 1 4 2 3 0 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcagtgttaactgg tggagttgggtccgccagcccccagggaaggggctggagtggattggggag atctctcacagtgggaacacc aactacagcccgtccctcaagggtcgagtcaccatatcaataaacaagtccaagaaccaattctccctgaagctgagctctgtgaccgccgcggacacggccgtgtattactgt gcgagaggggaaatgactgccgagtactactttgattac ARGEMTAEYYFDY IGHV4-4 IGHD2-21 IGHJ4 ggtggctccatcagcagtgttaactgg tggagttgggtccgccagcccccagggaaggggctggagtggattggggag atctctcacagtgggaacacc aactacagcccgtccctcaagggtcgagtcaccatatcaataaacaagtccaagaaccaattctccctgaagctgagctctgtgaccgccgcggacacggccgtgtattactgt gcgagaggggaaatgactgccgagtactactttgattac IGA2 11 213 9 2 4 5 5 6 2 1 4 4 JY8QFUQ01A6IZH 19233 productive (see comment) Homsap IGHV3-7*01 F 891 89.86 195/217 nt 89.4 194/217 nt Homsap IGHJ1*01 F (see comment) 161 78.85 41/52 nt Homsap IGHD6-19*01 F 1 8 8 10 8.8.10 [1.17.38.11] CVVSSGWLFHYW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in CDR2-IMGT at codon 65 of V-REGION: 'G' (1 nucleotides at position 101 to 101 in the user submitted sequence), (cause frameshift); ctctggattcacctctcctagatactggatgaattgggtccgccaggcttccgggaaggggctggagtgggtggccaacataaagcaagacggaagtgagGaaaaactttgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaattcaatgtctctacaaatgaacagcctgagagtcgaggacacggctgtatattattgcgttgttagcagtggctggttgttccactactggggccagggctcccgggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [280] t89>c,F30>S(- - -)|a103>c,S35>P(+ - -)|g104>c,S35>P(+ - -)|c108>a,S36>R(- - -)|t111>c|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|c136>t,P46>S(+ - -)|a138>c,P46>S(+ - -)|t177>c|t196>a,Y66>N(- - -)|a200>t,Y67>F(- + -)|a225>g|a245>g,N82>S(- - -)|c255>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|c287>t,A96>V(+ - +)|g303>a|c309>t| t89>c,F30>S(- - -)|a103>c,S35>P(+ - -)|g104>c,S35>P(+ - -)|c108>a,S36>R(- - -)|t111>c| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|c136>t,P46>S(+ - -)|a138>c,P46>S(+ - -)| t177>c| t196>a,Y66>N(- - -)|a200>t,Y67>F(- + -)|a225>g|a245>g,N82>S(- - -)|c255>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|c287>t,A96>V(+ - +)|g303>a|c309>t|t312>c| c314>t,A105>V(+ - +)|g315>t,A105>V(+ - +)|a316>g,R106>V(- - -)|g317>t,R106>V(- - -)|a318>t,R106>V(- - -)|g319>a|a320>g| 311 (320) 216 (225) 195 (196) 21 (29) 7 (10) 14 (19) 2 (4) 3 (4) 5 (6) 3 (4) 3 2 1 (2) 1 1 0 0 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 0 0 2 1 1 0 0 1 0 0 0 51 51 47 4 0 4 0 1 2 0 1 0 0 0 0 0 0 0 30 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 116 (117) 114 102 11 5 6 2 2 3 0 1 1 1 1 0 0 0 0 0 (8) 0 0 (1) 0 (7) 0 (2) 0 (5) 0 (2) 0 (1) 0 (1) 0 (0) 0 0 0 (1) 0 0 0 0 (2) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctctcctagatactgg atgaattgggtccgccaggcttccgggaaggggctggagtgggtggccaac ataaagcaagacggaagtgagaaa aactttgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaattcaatgtctctacaaatgaacagcctgagagtcgaggacacggctgtatattattgc gttgttagcagtggctggttgttccactac VVSSGWLFHY IGHV3-7 IGHD6-19 IGHJ1 ggattcacctctcctagatactgg atgaattgggtccgccaggcttccgggaaggggctggagtgggtggccaac ataaagcaagacggaagtgagaaa aactttgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaattcaatgtctctacaaatgaacagcctgagagtcgaggacacggctgtatattattgc gttgttagcagtggctggttgttccactac IGG2 21 213 13 8 8 11 5 10 5 2 10 4 JY8QFUQ01A6JX4 22193 productive Homsap IGHV3-48*02 F 891 90.32 196/217 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 210 90.2 46/51 nt Homsap IGHD5-24*01 ORF 2 8 8 15 8.8.15 [1.17.38.11] CARGQKWLQVLRWFDSW in-frame - Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcaccttcgacagatacagtatgaactgggtccgccaggctccagggaggggactggagtggatttcatacataagtactactactagtaacagatactacgcagacgctgtgaagggccgattcaccatctccagagacaatgccaagaactcgctgtatttgcaaatggacagcctgagagacgaggacacggctgtatattattgtgcgagaggtcaaaaatggctacaagtgcttcgctggttcgactcctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggggcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [294] a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|t105>c,S35>D(- - -)|c108>a,S36>R(- - -)|t111>c|c114>t|a143>g,K48>R(+ + +)|g147>a|g157>a,V53>I(+ - +)|t168>a|g173>c,S58>T(+ - +)|g176>c,S59>T(+ - +)|g185>c,S62>T(+ - +)|c191>a,T64>N(- + -)|t194>g,I65>R(- + -)|t208>g,S70>A(- + -)|a258>g|c265>t|a274>g,N92>D(+ + -)|g303>a|c309>t| a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|t105>c,S35>D(- - -)|c108>a,S36>R(- - -)|t111>c|c114>t| a143>g,K48>R(+ + +)|g147>a|g157>a,V53>I(+ - +)| t168>a|g173>c,S58>T(+ - +)|g176>c,S59>T(+ - +)|g185>c,S62>T(+ - +)|c191>a,T64>N(- + -)|t194>g,I65>R(- + -)| t208>g,S70>A(- + -)|a258>g|c265>t|a274>g,N92>D(+ + -)|g303>a|c309>t| a320>g| 319 (320) 224 (225) 203 21 (22) 8 (9) 13 4 (5) 4 3 2 0 2 0 1 3 0 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 2 4 1 1 1 2 0 1 0 0 0 0 0 0 51 51 48 3 1 2 1 2 0 0 0 0 0 0 0 0 0 0 30 24 18 6 1 5 0 0 0 0 0 1 0 1 3 0 0 1 117 114 108 6 4 2 2 1 2 0 0 0 0 0 0 0 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ctct ggattcaccttcgacagatacagt atgaactgggtccgccaggctccagggaggggactggagtggatttcatac ataagtactactactagtaacaga tactacgcagacgctgtgaagggccgattcaccatctccagagacaatgccaagaactcgctgtatttgcaaatggacagcctgagagacgaggacacggctgtatattattgt gcgagaggtcaaaaatggctacaagtgcttcgctggttcgactcc ARGQKWLQVLRWFDS IGHV3-48 IGHD5-24 IGHJ5 ggattcaccttcgacagatacagt atgaactgggtccgccaggctccagggaggggactggagtggatttcatac ataagtactactactagtaacaga tactacgcagacgctgtgaagggccgattcaccatctccagagacaatgccaagaactcgctgtatttgcaaatggacagcctgagagacgaggacacggctgtatattattgt gcgagaggtcaaaaatggctacaagtgcttcgctggttcgactcc IGG1 21 213 13 8 7 12 6 9 5 3 4 9 JY8QFUQ01A6L65 20668 productive Homsap IGHV3-9*01 F 932 92.2 201/218 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 2 8 8 16 8.8.16 [1.17.38.11] CAKDGSAIPVTGTYFEYW in-frame + cctctggattcacctttcatgattataccatgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagtattagttggaatagtggtaacatagactatgcggcctctgtgaggggccgattcaccgtctccagagacaacgccaataactccctgtctcttcagatgaatggtctgagatctgaggacacggccctctattactgtgcaaaagacgggtcggcgataccagtgactggtacctactttgaatactggggccagggagccctggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaaactcagg NA IGG4 100 88 [298] g103>c,D35>H(- - -)|g112>a,A38>T(- - -)|g163>a,G55>S(+ + -)|g191>a,S64>N(- - -)|g197>a,G66>D(- - -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|g252>t,K84>N(+ - -)|a263>c,Y88>S(+ - -)|g267>t|a270>g|c276>t|a277>g,S93>G(+ + -)|g286>t,A96>S(- + -)|t301>c|g303>c| g103>c,D35>H(- - -)|g112>a,A38>T(- - -)| g163>a,G55>S(+ + -)| g191>a,S64>N(- - -)| g197>a,G66>D(- - -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|g252>t,K84>N(+ - -)|a263>c,Y88>S(+ - -)|g267>t|a270>g|c276>t|a277>g,S93>G(+ + -)|g286>t,A96>S(- + -)|t301>c|g303>c| t321>c|a322>g| 320 (322) 226 (228) 209 17 (19) 5 (7) 12 4 (5) 4 1 1 (2) 2 0 0 0 2 0 3 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 117 114 101 13 5 8 4 1 1 1 2 0 0 0 1 0 3 0 8 (10) 8 8 0 (2) 0 (2) 0 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttcatgattatacc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt attagttggaatagtggtaacata gactatgcggcctctgtgaggggccgattcaccgtctccagagacaacgccaataactccctgtctcttcagatgaatggtctgagatctgaggacacggccctctattactgt gcaaaagacgggtcggcgataccagtgactggtacctactttgaatac AKDGSAIPVTGTYFEY IGHV3-9 IGHD6-19 IGHJ4 ggattcacctttcatgattatacc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt attagttggaatagtggtaacata gactatgcggcctctgtgaggggccgattcaccgtctccagagacaacgccaataactccctgtctcttcagatgaatggtctgagatctgaggacacggccctctattactgt gcaaaagacgggtcggcgataccagtgactggtacctactttgaatac IGG4 17 213 10 7 5 10 5 7 5 0 9 3 JY8QFUQ01A6LFT 5107 productive Homsap IGHV1-18*01 F 957 94.39 202/214 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD1-26*01 F 1 8 8 12 8.8.12 [0.17.38.11] CATSDVGARKFDYW in-frame + tggttacacctttaccagatatggtattagttgggtgcgacaggcccctggacaagggcttgagtggatgggatggatcagcgtttccaatggtgacacaaactatgcacagaagctccagggcagagtcaccatgaccgcagacccatccacgagcacagcctacttggaactgaggagcctgacatctgacgacacggccgtatattactgtgcgacatctgacgttggagctcgaaagtttgactactggggccagggaacccaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [281] c108>a,S36>R(- - -)|c117>t|c120>t|c173>t,A58>V(+ - +)|a176>c,Y59>S(+ - -)|a190>g,N64>D(+ + -)|a238>g,T80>A(- - -)|a244>c,T82>P(+ + -)|a265>t,M89>L(+ + -)|g270>a|g284>c,R95>T(- - -)|g303>a| c108>a,S36>R(- - -)| c117>t|c120>t| c173>t,A58>V(+ - +)|a176>c,Y59>S(+ - -)|a190>g,N64>D(+ + -)| a238>g,T80>A(- - -)|a244>c,T82>P(+ + -)|a265>t,M89>L(+ + -)|g270>a|g284>c,R95>T(- - -)|g303>a| g317>c,R106>T(- - -)|g319>t|a320>c| 316 (320) 218 (222) 206 (207) 12 (15) 4 (6) 8 (9) 2 2 3 0 2 (3) 1 1 0 1 (2) 0 0 (1) 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 49 2 2 0 0 0 2 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 1 0 1 0 0 0 0 0 0 0 117 114 108 6 2 4 1 2 0 0 1 0 1 0 1 0 0 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 (1) 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|ta,175-176(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,5-6(FR1)|tt,75-76(FR1)|tt,81-82(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|tt,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,3-6(FR1)|agct,8-11(FR1)|agct,24-27(FR1)|ggct,72-75(FR1)|ggtt,79-82(CDR1)|agct,106-109(CDR1)|ggta,112-115(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)| agct,8-11(FR1)|agct,24-27(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|agct,209-212(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggttacacctttaccagatatggt attagttgggtgcgacaggcccctggacaagggcttgagtggatgggatgg atcagcgtttccaatggtgacaca aactatgcacagaagctccagggcagagtcaccatgaccgcagacccatccacgagcacagcctacttggaactgaggagcctgacatctgacgacacggccgtatattactgt gcgacatctgacgttggagctcgaaagtttgactac ATSDVGARKFDY IGHV1-18 IGHD1-26 IGHJ4 ggttacacctttaccagatatggt attagttgggtgcgacaggcccctggacaagggcttgagtggatgggatgg atcagcgtttccaatggtgacaca aactatgcacagaagctccagggcagagtcaccatgaccgcagacccatccacgagcacagcctacttggaactgaggagcctgacatctgacgacacggccgtatattactgt gcgacatctgacgttggagctcgaaagtttgactac IGA2 12 213 7 5 5 7 2 5 4 0 4 4 JY8QFUQ01A6MYW 14527 productive (see comment) Homsap IGHV3-49*03 F, or Homsap IGHV3-49*05 F 1033 96.4 214/222 nt 95.5 212/222 nt Homsap IGHJ4*02 F (see comment) 177 85.42 41/48 nt Homsap IGHD3-3*01 F 1 7 10 17 7.10.17 [1.17.38.11] CTRDNPPLRSLEWLPFDSW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT at codon 37 of V-REGION: 'G' (1 nucleotides at position 24 to 24 in the user submitted sequence), (cause frameshift); in CDR1-IMGT, from codon 28 of V-REGION: 1 nucleotides (from position 9 in the user submitted sequence), (cause frameshift) ttctggatcacctttggtgattaGtagtatgagttggttccgccaggctccagggaaggggctggagtgggtcggtttcattagaagcaaagctgatgatgggacaacagaatacgccgcgtctgtgaaaggcagattcaccatctcaagagatgattccaaaagcatcgcctatctgcaaatgaatagcctgaaaaccggggacacagccgtgtattactgtactagagataaccctccattgcgatctttggagtggttaccctttgactcctggggccagggaaccctagtcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [305] g112>a,A38>S(- + -)|c113>g,A38>S(- + -)|c120>t|a159>c|t181>g,Y61>D(- - -)|g185>a,G62>D(- - -)|c276>t|a290>g,E97>G(- - -)| g112>a,A38>S(- + -)|c113>g,A38>S(- + -)| c120>t|a159>c| t181>g,Y61>D(- - -)|g185>a,G62>D(- - -)| c276>t|a290>g,E97>G(- - -)| 320 230 222 8 3 5 1 2 2 0 1 0 0 0 0 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 21 2 0 2 0 1 0 0 0 0 0 0 0 1 0 0 51 51 49 2 2 0 0 0 1 0 1 0 0 0 0 0 0 0 30 30 28 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 117 114 112 2 1 1 1 0 1 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,158-159(FR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,181-182(CDR2)|aa,192-193(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|ta,69-70(FR1)|tt,75-76(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,124-125(FR2)|ta,158-159(FR2)|tt,162-163(FR2)|tt,167-168(CDR2)|tt,180-181(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agct,72-75(FR1)|agct,118-121(FR2)|ggtt,122-125(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggta,156-159(FR2)|ggtt,160-163(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|ggca,220-223(FR3)|agca,253-256(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,72-75(FR1)|tgct,111-114(CDR1)|agct,118-121(FR2)|agct,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| ttct ggatcacctttggtgattatagt atgagttggttccgccaggctccagggaaggggctggagtgggtcggtttc attagaagcaaagctgatgatgggacaaca gaatacgccgcgtctgtgaaaggcagattcaccatctcaagagatgattccaaaagcatcgcctatctgcaaatgaatagcctgaaaaccggggacacagccgtgtattactgt actagagataaccctccattgcgatctttggagtggttaccctttgactcc TRDNPPLRSLEWLPFDS IGHV3-49 IGHD3-3 IGHJ4 ggatcacctttggtgattatagt atgagttggttccgccaggctccagggaaggggctggagtgggtcggtttc attagaagcaaagctgatgatgggacaaca gaatacgccgcgtctgtgaaaggcagattcaccatctcaagagatgattccaaaagcatcgcctatctgcaaatgaatagcctgaaaaccggggacacagccgtgtattactgt actagagataaccctccattgcgatctttggagtggttaccctttgactcc IGA1 8 218 5 3 4 5 1 3 3 0 1 4 JY8QFUQ01A6PW0 4670 productive Homsap IGHV3-30*01 F, or Homsap IGHV3-30*11 F 967 94.44 204/216 nt NA Homsap IGHJ6*02 F (see comment) 202 80.65 50/62 nt Homsap IGHD2-2*01 F 2 8 8 14 8.8.14 [1.17.38.11] CVREGRGTAAYGLDVW in-frame + Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) tctggattcaccttcagtagctatgctatgcactgggtccgccagactccaggcaagggactagagtgggtggcagttatatcatatgatggaagtgactacgactacgcaggctccgtgaagggccgattcaccatctccagagacagttccaagaacatgctgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtatcactgtgtgagagagggtcgtggtaccgccgcctacggtctggacgtctggggccgagggaccacggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttgccccgaaccggtgacggtgtcgtggcaantca NA IGG3 100 88 [290] g133>a,A45>T(- - -)|g147>a|a190>g,N64>D(+ + -)|t192>c,N64>D(+ + -)|a193>t,K65>Y(- - -)|a195>c,K65>Y(- - -)|t196>g,Y66>D(- - -)|a206>g,D69>G(- - -)|a245>g,N82>S(- - -)|c257>t,T86>M(- - -)|g286>c,A96>P(- - -)|t307>c,Y103>H(+ - -)|c314>t,A105>V(+ - +)| g133>a,A45>T(- - -)|g147>a| a190>g,N64>D(+ + -)|t192>c,N64>D(+ + -)|a193>t,K65>Y(- - -)|a195>c,K65>Y(- - -)| t196>g,Y66>D(- - -)|a206>g,D69>G(- - -)|a245>g,N82>S(- - -)|c257>t,T86>M(- - -)|g286>c,A96>P(- - -)|t307>c,Y103>H(+ - -)| c314>t,A105>V(+ - +)| 320 224 211 13 1 12 3 2 2 2 1 0 1 0 1 0 0 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 0 1 1 0 1 0 0 0 0 0 117 114 108 6 0 6 2 0 1 1 0 0 0 0 1 0 0 1 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcagtagctatgct atgcactgggtccgccagactccaggcaagggactagagtgggtggcagtt atatcatatgatggaagtgactac gactacgcaggctccgtgaagggccgattcaccatctccagagacagttccaagaacatgctgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtatcactgt gtgagagagggtcgtggtaccgccgcctacggtctggacgtc VREGRGTAAYGLDV IGHV3-30 IGHD2-2 IGHJ6 ggattcaccttcagtagctatgct atgcactgggtccgccagactccaggcaagggactagagtgggtggcagtt atatcatatgatggaagtgactac gactacgcaggctccgtgaagggccgattcaccatctccagagacagttccaagaacatgctgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtatcactgt gtgagagagggtcgtggtaccgccgcctacggtctggacgtc IGG3 12 213 8 4 3 4 5 8 1 0 7 4 JY8QFUQ01A6T5J 6539 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30*19 F or Homsap IGHV3-30-5*01 F 860 88.53 193/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-3*01 F 2 8 8 10 8.8.10 [1.17.38.11] CAKDFYYGCDYW in-frame + tctctggattcaacttcagtggctatggcatgcactgggtccgccaggctccaggcaagggcctggagtgggtggcagtcattgcatacaatggaggaaatatatactacgcagactccgtgaagggccgattcaccatctccagagacaattccaaggataccctgtacttgcacatgaccagtctgagacctgatgacacggccatgtattactgtgcgaaagacttttattatggttgcgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccga NA IGA1 100 100 [279] c74>t|c86>a,T29>N(- + -)|a106>g,S36>G(+ + -)|g147>c|t165>c|a168>t|t169>g,S57>A(- + -)|t174>c|g175>a,D59>N(+ + -)|a187>g,S63>G(+ + -)|t189>a,S63>G(+ + -)|a194>t,K65>I(- + -)|t201>c|a253>g,N85>D(+ + -)|c255>t,N85>D(+ + -)|g258>c|t264>c|c265>t|a270>c,Q90>H(- + -)|a275>c,N92>T(- + -)|c279>t|g286>c,A96>P(- - -)|g291>t,E97>D(+ - +)|t300>c|g301>a,V101>M(+ - -)|g317>a,R106>K(+ + +)| c74>t| c86>a,T29>N(- + -)|a106>g,S36>G(+ + -)| g147>c|t165>c| a168>t|t169>g,S57>A(- + -)|t174>c|g175>a,D59>N(+ + -)|a187>g,S63>G(+ + -)|t189>a,S63>G(+ + -)|a194>t,K65>I(- + -)| t201>c|a253>g,N85>D(+ + -)|c255>t,N85>D(+ + -)|g258>c|t264>c|c265>t|a270>c,Q90>H(- + -)|a275>c,N92>T(- + -)|c279>t|g286>c,A96>P(- - -)|g291>t,E97>D(+ - +)|t300>c|g301>a,V101>M(+ - -)| g317>a,R106>K(+ + +)| 320 226 200 26 11 15 3 3 4 5 2 1 2 1 3 0 1 1 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 1 0 0 0 0 0 0 51 51 49 2 2 0 0 0 0 1 0 0 0 0 1 0 0 0 30 24 17 7 2 5 1 1 0 1 0 0 2 1 0 0 0 1 117 114 101 13 6 7 1 1 3 3 2 0 0 0 2 0 1 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| tctct ggattcaacttcagtggctatggc atgcactgggtccgccaggctccaggcaagggcctggagtgggtggcagtc attgcatacaatggaggaaatata tactacgcagactccgtgaagggccgattcaccatctccagagacaattccaaggataccctgtacttgcacatgaccagtctgagacctgatgacacggccatgtattactgt gcgaaagacttttattatggttgcgactac AKDFYYGCDY IGHV3-30 IGHD3-3 IGHJ4 ggattcaacttcagtggctatggc atgcactgggtccgccaggctccaggcaagggcctggagtgggtggcagtc attgcatacaatggaggaaatata tactacgcagactccgtgaagggccgattcaccatctccagagacaattccaaggataccctgtacttgcacatgaccagtctgagacctgatgacacggccatgtattactgt gcgaaagacttttattatggttgcgactac IGA1 24 213 13 11 5 10 8 14 8 2 7 7 JY8QFUQ01A6UEX 7036 productive Homsap IGHV3-21*04 F 990 95.39 207/217 nt NA Homsap IGHJ6*02 F, or Homsap IGHJ6*03 F 172 78.57 44/56 nt Homsap IGHD6-13*01 F 3 8 8 10 8.8.10 [1.17.38.11] CARAVSNQVDVW in-frame + ctctggattcgccttcagtacatatatcatgaattgggtccgccaggctccagggaaggggctggagtgggtctcatccattagttttagtagtgattacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgacgacacggccgtgtattactgtgcgagagccgtttcgaatcaggtggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [278] a85>g,T29>A(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|g113>t,S38>I(- - -)|c120>t|a172>t,S58>F(- - -)|g173>t,S58>F(- - -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|g291>c,E97>D(+ - +)| a85>g,T29>A(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|g113>t,S38>I(- - -)| c120>t| a172>t,S58>F(- - -)|g173>t,S58>F(- - -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)| g291>c,E97>D(+ - +)| a320>c| 319 (320) 224 (225) 214 10 (11) 1 (2) 9 2 1 1 0 0 (1) 1 1 0 2 0 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 0 0 0 0 1 0 0 1 0 1 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 1 0 0 0 0 1 0 0 0 1 0 117 114 113 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcgccttcagtacatatatc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagttttagtagtgattacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgacgacacggccgtgtattactgt gcgagagccgtttcgaatcaggtggacgtc ARAVSNQVDV IGHV3-21 IGHD6-13 IGHJ6 ggattcgccttcagtacatatatc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagttttagtagtgattacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgacgacacggccgtgtattactgt gcgagagccgtttcgaatcaggtggacgtc IGA2 10 213 4 6 2 7 2 3 1 0 1 8 JY8QFUQ01A6V15 4177 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 833 87.16 190/218 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-16*02 F 3 8 8 11 8.8.11 [1.17.38.11] CAKGQFGTVMDYW in-frame + cctctggattcacctttaacgactatggcatgacttgggtccgccaggctccagggatggggctgcagtgggtcgcaaccattagtggaagtggcgacagaacattctacgcagactccgtgaagggccgcttcatcgtttccagagacaactccaagaacacgctgtatctgcaaattaccagcccgagagccgaggacacggccgttttttattgtgcgaaaggccagtttggtaccgtcatggactattggggccagggaacccaggtcgtcgtctcctcagcatcccgaccagcccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccg NA IGA2 100 89 [280] g104>a,S35>N(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c113>g,A38>G(- + -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|a143>t,K48>M(- + -)|g151>c,E51>Q(+ + -)|t160>g,S54>A(- + -)|g163>a,A55>T(- - -)|t165>c,A55>T(- - -)|t174>a|t186>c|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|c192>a,S64>R(- - -)|a197>t,Y66>F(- + -)|g225>c|c230>t,T77>I(- - -)|a232>g,I78>V(+ - +)|c234>t,I78>V(+ - +)|t246>c|g273>t,M91>I(+ + -)|a275>c,N92>T(- + -)|t281>c,L94>P(- - -)|a303>t|a305>t,Y102>F(- + -)|c309>t| g104>a,S35>N(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c113>g,A38>G(- + -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|a143>t,K48>M(- + -)|g151>c,E51>Q(+ + -)|t160>g,S54>A(- + -)|g163>a,A55>T(- - -)|t165>c,A55>T(- - -)| t174>a|t186>c|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|c192>a,S64>R(- - -)| a197>t,Y66>F(- + -)|g225>c|c230>t,T77>I(- - -)|a232>g,I78>V(+ - +)|c234>t,I78>V(+ - +)|t246>c|g273>t,M91>I(+ + -)|a275>c,N92>T(- + -)|t281>c,L94>P(- - -)|a303>t|a305>t,Y102>F(- + -)|c309>t| a320>g| 319 (320) 225 (226) 197 28 (29) 6 (7) 22 2 (3) 4 4 5 1 1 4 1 3 1 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 2 0 0 0 0 0 0 0 1 0 0 51 51 44 7 0 7 0 1 1 1 0 0 1 0 2 0 0 1 30 24 19 5 2 3 0 1 0 2 0 1 0 1 0 0 0 0 117 114 102 12 4 8 1 0 3 2 1 0 3 0 1 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttaacgactatggc atgacttgggtccgccaggctccagggatggggctgcagtgggtcgcaacc attagtggaagtggcgacagaaca ttctacgcagactccgtgaagggccgcttcatcgtttccagagacaactccaagaacacgctgtatctgcaaattaccagcccgagagccgaggacacggccgttttttattgt gcgaaaggccagtttggtaccgtcatggactat AKGQFGTVMDY IGHV3-23 IGHD3-16 IGHJ4 ggattcacctttaacgactatggc atgacttgggtccgccaggctccagggatggggctgcagtgggtcgcaacc attagtggaagtggcgacagaaca ttctacgcagactccgtgaagggccgcttcatcgtttccagagacaactccaagaacacgctgtatctgcaaattaccagcccgagagccgaggacacggccgttttttattgt gcgaaaggccagtttggtaccgtcatggactat IGA2 28 213 15 13 8 14 7 14 4 2 15 7 JY8QFUQ01A6VGQ 9764 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 941 92.66 202/218 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD3-16*01 F 2 8 8 16 8.8.16 [1.17.38.11] CARRLNNYDSGEYAMDVW in-frame + gttctggatacacgtttatgaattactggatcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatcatctatcctggtacctctgagaccagatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgccttcgtgcagtggagcagcctgaaggccccggacaccgccatatattactgtgcgaggcgactcaataattacgactcgggagagtacgctatggacgtctggggccaagggaccacggtcaccgtctcctcaacctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaac NA IGG1 100 100 [298] g86>c,S29>T(+ - +)|c87>g,S29>T(+ - +)|c104>t,T35>M(- - -)|c105>g,T35>M(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g184>a,D62>T(- + -)|a185>c,D62>T(- + -)|t192>g,D64>E(+ - +)|c213>t|c234>t|t251>c,I84>T(- - -)|a263>t,Y88>F(- + -)|c265>g,L89>V(+ - +)|t289>c,S97>P(+ - -)|g303>a,M101>I(+ + -)| g86>c,S29>T(+ - +)|c87>g,S29>T(+ - +)|c104>t,T35>M(- - -)|c105>g,T35>M(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g184>a,D62>T(- + -)|a185>c,D62>T(- + -)|t192>g,D64>E(+ - +)| c213>t|c234>t|t251>c,I84>T(- - -)|a263>t,Y88>F(- + -)|c265>g,L89>V(+ - +)|t289>c,S97>P(+ - -)|g303>a,M101>I(+ + -)| a318>g|a320>g| 317 (320) 223 (226) 207 (208) 16 (18) 2 (4) 14 0 (2) 3 4 2 1 0 1 0 1 3 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 1 2 0 0 0 0 0 1 2 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 1 0 0 1 0 0 0 0 0 0 1 117 114 107 7 2 5 0 1 2 2 0 0 1 0 0 1 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| gttct ggatacacgtttatgaattactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtacctctgagacc agatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgccttcgtgcagtggagcagcctgaaggccccggacaccgccatatattactgt gcgaggcgactcaataattacgactcgggagagtacgctatggacgtc ARRLNNYDSGEYAMDV IGHV5-51 IGHD3-16 IGHJ6 ggatacacgtttatgaattactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtacctctgagacc agatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgccttcgtgcagtggagcagcctgaaggccccggacaccgccatatattactgt gcgaggcgactcaataattacgactcgggagagtacgctatggacgtc IGG1 16 213 9 7 7 11 2 5 2 0 5 9 JY8QFUQ01A6VPK 8002 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 872 89.77 193/215 nt 89.3 192/215 nt Homsap IGHJ3*02 F 106 68 34/50 nt Homsap IGHD2-15*01 F 2 8 7 20 8.7.20 [1.17.38.11] CARDPRDYCSGGYCFVGAFDIW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'T' (1 nucleotides at position 48 to 48 in the user submitted sequence), (cause frameshift); tctctggtggctccatgaggaattattactggagctggatccggcagTcccccagggaagggactggagttgatagggactgtctattacactgggcgcacggagtacaacccctccctcaagagtcgactcaccttatcactagacacgtccaagaaccagttctccctaaagctgggctctgtgaccgctgcggactcggccatttattactgtgcgagagacccccgagactattgtagtggtggttactgttttgttggtgcttttgatatctggggccaagggacaaatggtcaccgtctcttcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [309] c90>g,I30>M(+ + -)|t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t|g155>t,W52>L(+ - -)|t159>a|t163>a,Y55>T(+ - -)|a164>c,Y55>T(+ - -)|a166>g,I56>V(+ - +)|g176>c,S59>T(+ - +)|a190>c,S64>R(- - -)|c195>g|a196>g,N66>E(+ - -)|c198>g,N66>E(+ - -)|g226>c,V76>L(+ - +)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|g267>a|a274>g,S92>G(+ + -)|a295>t,T99>S(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| c90>g,I30>M(+ + -)|t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t| g155>t,W52>L(+ - -)|t159>a|t163>a,Y55>T(+ - -)|a164>c,Y55>T(+ - -)| a166>g,I56>V(+ - +)|g176>c,S59>T(+ - +)|a190>c,S64>R(- - -)|c195>g| a196>g,N66>E(+ - -)|c198>g,N66>E(+ - -)|g226>c,V76>L(+ - +)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|g267>a|a274>g,S92>G(+ + -)|a295>t,T99>S(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| 320 223 201 22 4 18 3 3 1 0 2 0 2 2 3 3 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 1 0 0 0 0 0 0 1 0 1 51 51 47 4 1 3 0 0 0 0 1 0 0 2 0 0 1 0 30 21 17 4 1 3 1 0 0 0 1 0 0 0 1 1 0 0 117 114 104 10 1 9 2 2 0 0 0 0 2 0 2 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatgaggaattattac tggagctggatccggcagcccccagggaagggactggagttgatagggact gtctattacactgggcgcacg gagtacaacccctccctcaagagtcgactcaccttatcactagacacgtccaagaaccagttctccctaaagctgggctctgtgaccgctgcggactcggccatttattactgt gcgagagacccccgagactattgtagtggtggttactgttttgttggtgcttttgatatc ARDPRDYCSGGYCFVGAFDI IGHV4-59 IGHD2-15 IGHJ3 ggtggctccatgaggaattattac tggagctggatccggcagcccccagggaagggactggagttgatagggact gtctattacactgggcgcacg gagtacaacccctccctcaagagtcgactcaccttatcactagacacgtccaagaaccagttctccctaaagctgggctctgtgaccgctgcggactcggccatttattactgt gcgagagacccccgagactattgtagtggtggttactgttttgttggtgcttttgatatc IGG1 22 210 7 15 4 12 3 10 2 2 12 6 JY8QFUQ01A6W08 6301 productive (see comment) Homsap IGHV3-7*01 F 967 94.44 204/216 nt 93.98 203/216 nt Homsap IGHJ4*02 F 176 86.96 40/46 nt Homsap IGHD7-27*01 F 1 8 8 6 8.8.6 [1.17.37.11] CSRLLEEW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 66 of V-REGION: 1 nucleotides (from position 104 in the user submitted sequence), (cause frameshift) ctctggtttcacctttagtaacgattggatggactgggtccgccaggctccagggaaggggctggagtgggtggccaatataaagggagatggaagtgagaaaactatgtagactctgcgaagggccgattcatcatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgttcgagactccttgaggaatggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccg NA IGA1 100 100 [265] a81>t|g107>a,S36>N(- - -)|t109>g,Y37>D(- - -)|a118>g,S40>D(- - -)|g119>a,S40>D(- - -)|c165>t|c172>g,Q58>G(- - -)|a173>g,Q58>G(- - -)|g204>a|t212>c,V71>A(+ - +)|c230>t,T77>I(- - -)|c287>t,A96>V(+ - +)|g313>t,A105>S(- + -)| a81>t|g107>a,S36>N(- - -)|t109>g,Y37>D(- - -)| a118>g,S40>D(- - -)|g119>a,S40>D(- - -)|c165>t| c172>g,Q58>G(- - -)|a173>g,Q58>G(- - -)| g204>a|t212>c,V71>A(+ - +)|c230>t,T77>I(- - -)|c287>t,A96>V(+ - +)| g313>t,A105>S(- + -)|g319>c|a320>t| 318 (320) 222 (224) 209 13 (15) 3 (5) 10 2 3 3 1 0 0 1 (2) 0 0 (1) 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 0 0 0 0 1 0 0 0 0 1 51 51 48 3 1 2 1 1 1 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 1 0 0 117 113 109 4 1 3 0 1 2 1 0 0 0 0 0 0 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 0 0 0 0 0 0 (1) 0 0 (1) 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggtttcacctttagtaacgattgg atggactgggtccgccaggctccagggaaggggctggagtgggtggccaat ataaagggagatggaagtgagaaa actatgtagactctgcgaagggccgattcatcatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgt tcgagactccttgaggaa SRLLEE IGHV3-7 IGHD7-27 IGHJ4 ggtttcacctttagtaacgattgg atggactgggtccgccaggctccagggaaggggctggagtgggtggccaat ataaagggagatggaagtgagaaa actatgtagactctgcgaagggccgattcatcatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgt tcgagactccttgaggaa IGA1 12 212 9 3 6 7 3 5 2 1 5 4 JY8QFUQ01A6XI0 1782 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 927 92.17 200/217 nt NA Homsap IGHJ3*02 F 214 92 46/50 nt Homsap IGHD7-27*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARDQSLGMPEAFDIW in-frame + ctctggattcaccttcagtcgctatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcaactattagtagtcatagtatttacatatactatgcagactcagtggagggccgattcaccgtctccagagacaacgccgagaactcgctgtatctgcacatgaacaccctcagagccgacgacacggctatatattactgtgcgagagatcaaagtctggggatgcccgaggcttttgacatctggggccaagggacaatggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [291] a106>c,S36>R(- - -)|t163>a,S55>T(+ - +)|c165>t,S55>T(+ - +)|a175>c,S59>H(+ - -)|g176>a,S59>H(+ - -)|g188>t,S63>I(- - -)|c201>t|a214>g,K72>E(+ - -)|a232>g,I78>V(+ - +)|a250>g,K84>E(+ - -)|a258>g|a270>c,Q90>H(- + -)|g278>c,S93>T(+ - +)|g282>c|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| a106>c,S36>R(- - -)| t163>a,S55>T(+ - +)|c165>t,S55>T(+ - +)| a175>c,S59>H(+ - -)|g176>a,S59>H(+ - -)|g188>t,S63>I(- - -)| c201>t|a214>g,K72>E(+ - -)|a232>g,I78>V(+ - +)|a250>g,K84>E(+ - -)|a258>g|a270>c,Q90>H(- + -)|g278>c,S93>T(+ - +)|g282>c|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 225 208 17 3 14 4 3 2 0 3 0 0 1 3 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 1 0 0 0 0 1 0 0 0 0 30 24 21 3 0 3 0 1 0 0 1 0 0 0 0 0 1 0 117 114 103 11 3 8 4 2 1 0 1 0 0 0 3 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtcgctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtagtcatagtatttacata tactatgcagactcagtggagggccgattcaccgtctccagagacaacgccgagaactcgctgtatctgcacatgaacaccctcagagccgacgacacggctatatattactgt gcgagagatcaaagtctggggatgcccgaggcttttgacatc ARDQSLGMPEAFDI IGHV3-21 IGHD7-27 IGHJ3 ggattcaccttcagtcgctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtagtcatagtatttacata tactatgcagactcagtggagggccgattcaccgtctccagagacaacgccgagaactcgctgtatctgcacatgaacaccctcagagccgacgacacggctatatattactgt gcgagagatcaaagtctggggatgcccgaggcttttgacatc IGG2 17 213 9 8 5 9 4 8 3 0 10 4 JY8QFUQ01A6ZD5 22022 productive (see comment) Homsap IGHV3-7*01 F 999 95.85 208/217 nt 95.39 207/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 7 8 11 7.8.11 [1.17.38.11] CAISSGYYSTGYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 30 of V-REGION: 1 nucleotides (from position 17 in the user submitted sequence), (cause frameshift) gctctggattcagcttagttactattggatgagctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaaggaagatggaagtgagagacactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgtgcgattagtagtggttattactcgacgggctactggggccagggaaccctggtcaccgtctcctcagcatcccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccccag NA IGA1 100 95 [282] c74>g|c86>g,T29>S(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)|t196>c,Y66>H(+ - -)|a254>g,N85>S(- - -)|g303>a| c74>g| c86>g,T29>S(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)| c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)| t196>c,Y66>H(+ - -)|a254>g,N85>S(- - -)|g303>a| g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 316 (320) 221 (225) 212 9 (13) 2 (4) 7 (9) 2 (3) 2 (3) 0 1 0 0 1 (2) 0 0 3 0 (1) 0 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 23 20 3 0 3 0 1 0 0 0 0 1 0 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 1 0 0 117 114 111 3 1 2 1 1 0 1 0 0 0 0 0 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gctct ggattcagcttagttactattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgattagtagtggttattactcgacgggctac AISSGYYSTGY IGHV3-7 IGHD3-22 IGHJ4 ggattcagcttagttactattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgattagtagtggttattactcgacgggctac IGA1 8 212 5 3 2 4 3 4 1 0 2 5 JY8QFUQ01A6ZI7 3271 productive Homsap IGHV3-30*19 F 976 94.91 205/216 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD1-26*01 F 3 8 8 13 8.8.13 [1.17.38.11] CAKSPSQVGYYLDCW in-frame + tctggattcaccttcagtacctttggcatgcactgggtccgccaggctcccggcaaggggctggagtgggtggcaatcatatcaaatgatggaagtaagaaatactacgcagactccgtgaagggccgattcaccatttccagagaaaattccgagaacacgctgtatctgcaaatgagcagcctgagagctgaggacacggctgtgtattactgtgcgaaatcaccctcccaagtgggctactacttagactgttggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA2 71 100 [286] g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|a138>c|g163>a,V55>I(+ - +)|t165>c,V55>I(+ - +)|t172>a,Y58>N(- - -)|t192>g,N64>K(+ - -)|c234>t|c243>a,D81>E(+ - +)|a250>g,K84>E(+ - -)|a275>g,N92>S(- - -)| g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)| a138>c|g163>a,V55>I(+ - +)|t165>c,V55>I(+ - +)| t172>a,Y58>N(- - -)|t192>g,N64>K(+ - -)| c234>t|c243>a,D81>E(+ - +)|a250>g,K84>E(+ - -)|a275>g,N92>S(- - -)| g317>a,R106>K(+ + +)|g319>t|a320>c| 316 (320) 220 (224) 209 (210) 11 (14) 2 (4) 9 (10) 2 1 (2) 1 1 1 (2) 1 1 1 1 0 0 (1) 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 1 0 0 0 51 51 48 3 1 2 0 1 0 1 1 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 0 1 0 0 0 1 117 114 110 4 1 3 2 0 1 0 0 1 0 0 0 0 0 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 0 (1) 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcagtacctttggc atgcactgggtccgccaggctcccggcaaggggctggagtgggtggcaatc atatcaaatgatggaagtaagaaa tactacgcagactccgtgaagggccgattcaccatttccagagaaaattccgagaacacgctgtatctgcaaatgagcagcctgagagctgaggacacggctgtgtattactgt gcgaaatcaccctcccaagtgggctactacttagactgt AKSPSQVGYYLDC IGHV3-30 IGHD1-26 IGHJ4 ggattcaccttcagtacctttggc atgcactgggtccgccaggctcccggcaaggggctggagtgggtggcaatc atatcaaatgatggaagtaagaaa tactacgcagactccgtgaagggccgattcaccatttccagagaaaattccgagaacacgctgtatctgcaaatgagcagcctgagagctgaggacacggctgtgtattactgt gcgaaatcaccctcccaagtgggctactacttagactgt IGA2 11 213 5 6 2 4 3 7 2 0 5 4 JY8QFUQ01A70EP 7753 productive Homsap IGHV1-2*02 F, or Homsap IGHV1-2*04 F 795 85.98 184/214 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD4-17*01 F 2 8 8 11 8.8.11 [0.17.38.11] CARGFYGDHFDSW in-frame + tggatacaccttcagcgactactacatacactggctgcgacaggcccctggacaacgacttgagtggatgggatggctgaaccctaataatggtgacacagcttatggccagagctttcagggccgggtcaccatgaccagggacacgtccgtcagcacagtgtacgtggaggtgaggaggctgagatctgacgacacagccctttatttctgtgcgagaggcttttacggtgaccactttgactcctggggccagggaaccttggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [278] c104>g,T35>S(+ - +)|g107>a,G36>D(- - -)|t114>c|g117>a,M39>I(+ + -)|g124>c,V42>L(+ - +)|g145>c,G49>R(- - -)|g147>a,G49>R(- - -)|a166>c,I56>L(+ + +)|c168>g,I56>L(+ + +)|c177>t|g185>a,S62>N(- - -)|g191>a,G64>D(- - -)|a196>g,N66>A(- - -)|a197>c,N66>A(- - -)|c198>t,N66>A(- - -)|c203>g,A68>G(- + -)|a204>c,A68>G(- + -)|a209>g,K70>S(- - -)|g210>c,K70>S(- - -)|a223>c|a250>g,I84>V(+ - +)|c260>t,A87>V(+ - +)|c261>g,A87>V(+ - +)|a265>g,M89>V(+ - -)|c271>g,L91>V(+ - +)|c276>g,S92>R(- - -)|g297>a|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)|a308>t,Y103>F(- + -)| c104>g,T35>S(+ - +)|g107>a,G36>D(- - -)|t114>c| g117>a,M39>I(+ + -)|g124>c,V42>L(+ - +)|g145>c,G49>R(- - -)|g147>a,G49>R(- - -)| a166>c,I56>L(+ + +)|c168>g,I56>L(+ + +)|c177>t|g185>a,S62>N(- - -)|g191>a,G64>D(- - -)| a196>g,N66>A(- - -)|a197>c,N66>A(- - -)|c198>t,N66>A(- - -)|c203>g,A68>G(- + -)|a204>c,A68>G(- + -)|a209>g,K70>S(- - -)|g210>c,K70>S(- - -)|a223>c|a250>g,I84>V(+ - +)|c260>t,A87>V(+ - +)|c261>g,A87>V(+ - +)|a265>g,M89>V(+ - -)|c271>g,L91>V(+ - +)|c276>g,S92>R(- - -)|g297>a|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)|a308>t,Y103>F(- + -)| a320>g| 319 (320) 221 (222) 191 30 (31) 4 (5) 26 4 (5) 6 3 1 4 0 1 0 4 6 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 0 1 0 0 0 0 0 1 0 0 51 51 47 4 0 4 0 2 0 0 0 0 0 0 2 0 0 0 30 24 19 5 1 4 0 2 1 0 1 0 0 0 0 1 0 0 117 114 96 18 2 16 4 1 2 0 3 0 1 0 2 4 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatacaccttcagcgactactac atacactggctgcgacaggcccctggacaacgacttgagtggatgggatgg ctgaaccctaataatggtgacaca gcttatggccagagctttcagggccgggtcaccatgaccagggacacgtccgtcagcacagtgtacgtggaggtgaggaggctgagatctgacgacacagccctttatttctgt gcgagaggcttttacggtgaccactttgactcc ARGFYGDHFDS IGHV1-2 IGHD4-17 IGHJ4 ggatacaccttcagcgactactac atacactggctgcgacaggcccctggacaacgacttgagtggatgggatgg ctgaaccctaataatggtgacaca gcttatggccagagctttcagggccgggtcaccatgaccagggacacgtccgtcagcacagtgtacgtggaggtgaggaggctgagatctgacgacacagccctttatttctgt gcgagaggcttttacggtgaccactttgactcc IGA1 30 213 14 16 9 20 5 10 2 2 20 6 JY8QFUQ01A70L0 8999 productive Homsap IGHV3-48*02 F (see comment) 734 81.65 178/218 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-21*01 F 2 8 8 10 8.8.10 [1.17.38.11] CARSTDGYYDHW in-frame - Low V-REGION identity (81,65% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release cctctggattccccttcaatatatggagcatgaattgggtccgccaggctccggggaagggactggagttgatcgcatacatcacaagtgatgaaagaaccatatactacgcagactctgtgaagggccgcttcaccatctctagagacaatgccaggaacctagtacatctggaaatgaacagcctgagggacggcgatctggctatctattactgcgcgagaagcactgatggctattacgaccactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 100 100 [280] a85>c,T29>P(+ + -)|g104>a,S35>N(- - -)|g107>t,S36>I(- - -)|c108>a,S36>I(- - -)|a110>g,Y37>W(- + -)|t111>g,Y37>W(- + -)|c120>t|a138>g|g147>a|g155>t,W52>L(+ - -)|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|t160>g,S54>A(- + -)|t168>c|g170>c,S57>T(+ - +)|t171>a,S57>T(+ - +)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|a184>g,S62>E(- - -)|g185>a,S62>E(- - -)|t186>a,S62>E(- - -)|t189>a,S63>R(- - -)|a225>c|c237>t|a251>g,K84>R(+ + +)|t256>c,S86>L(- - -)|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t262>c,Y88>H(+ - -)|c268>g,Q90>E(+ + -)|a285>g|a290>g,E97>G(- - -)|g291>c,E97>G(- - -)|c294>t|a295>c,T99>L(- - -)|c296>t,T99>L(- - -)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)|t312>c| a85>c,T29>P(+ + -)|g104>a,S35>N(- - -)|g107>t,S36>I(- - -)|c108>a,S36>I(- - -)|a110>g,Y37>W(- + -)|t111>g,Y37>W(- + -)| c120>t|a138>g|g147>a|g155>t,W52>L(+ - -)|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|t160>g,S54>A(- + -)| t168>c|g170>c,S57>T(+ - +)|t171>a,S57>T(+ - +)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|a184>g,S62>E(- - -)|g185>a,S62>E(- - -)|t186>a,S62>E(- - -)|t189>a,S63>R(- - -)| a225>c|c237>t|a251>g,K84>R(+ + +)|t256>c,S86>L(- - -)|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t262>c,Y88>H(+ - -)|c268>g,Q90>E(+ + -)|a285>g|a290>g,E97>G(- - -)|g291>c,E97>G(- - -)|c294>t|a295>c,T99>L(- - -)|c296>t,T99>L(- - -)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)|t312>c| g319>a|a320>g| 318 (320) 224 (226) 184 40 (42) 9 (11) 31 7 (8) 7 (8) 5 5 3 1 0 3 3 2 2 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 1 0 0 1 1 0 0 0 0 1 1 51 51 44 7 3 4 1 2 1 1 0 0 0 0 0 0 1 1 30 24 15 9 1 8 2 2 0 1 0 0 0 3 1 0 0 0 117 114 96 18 5 13 3 2 4 3 2 0 0 0 2 2 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| cctct ggattccccttcaatatatggagc atgaattgggtccgccaggctccggggaagggactggagttgatcgcatac atcacaagtgatgaaagaaccata tactacgcagactctgtgaagggccgcttcaccatctctagagacaatgccaggaacctagtacatctggaaatgaacagcctgagggacggcgatctggctatctattactgc gcgagaagcactgatggctattacgaccac ARSTDGYYDH IGHV3-48 IGHD2-21 IGHJ4 ggattccccttcaatatatggagc atgaattgggtccgccaggctccggggaagggactggagttgatcgcatac atcacaagtgatgaaagaaccata tactacgcagactctgtgaagggccgcttcaccatctctagagacaatgccaggaacctagtacatctggaaatgaacagcctgagggacggcgatctggctatctattactgc gcgagaagcactgatggctattacgaccac IGG1 40 213 24 16 12 20 12 20 8 1 17 14 JY8QFUQ01A720R 11219 productive Homsap IGHV3-43*01 F 1004 95.87 209/218 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD2-8*01 F 1 8 8 13 8.8.13 [1.17.38.11] CAKDRIGSLYYFDHW in-frame + cctcaggattcacatttgctgaatataccatgcactgggtccgtcaagttccggggaagggtccggagtgggtctctcttattagttgggatggtggcagcacatactatgcagactctgtgaagggccgattcaccatctccagagacaacagcaaggactccctgtatctgcaaatgaacagtctgagaactgaggacaccgccttgtattactgtgcaaaagataggattgggtccttgtactactttgaccactggggccagggaaccctggtcaccgtctcctcagcatccccggaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttc NA IGA1 100 95 [289] t78>a|c87>a|a104>c,D35>A(- - -)|t108>a,D36>E(+ - +)|c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)|t189>c|a252>g|a253>g,N85>D(+ + -)| t78>a| c87>a|a104>c,D35>A(- - -)|t108>a,D36>E(+ - +)| c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)| t189>c| a252>g|a253>g,N85>D(+ + -)| 322 228 219 9 4 5 2 0 1 2 1 1 0 2 0 0 0 0 78 5 4 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 21 3 1 2 0 0 0 0 1 1 0 1 0 0 0 0 51 51 49 2 0 2 0 0 1 1 0 0 0 0 0 0 0 0 30 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 112 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 10 10 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,111-112(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggta,36-39(FR1)|agct,132-135(FR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|agca,247-250(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tacc,111-114(CDR1)|agct,132-135(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|aact,285-288(FR3)|tact,307-310(FR3)| cctca ggattcacatttgctgaatatacc atgcactgggtccgtcaagttccggggaagggtccggagtgggtctctctt attagttgggatggtggcagcaca tactatgcagactctgtgaagggccgattcaccatctccagagacaacagcaaggactccctgtatctgcaaatgaacagtctgagaactgaggacaccgccttgtattactgt gcaaaagataggattgggtccttgtactactttgaccac AKDRIGSLYYFDH IGHV3-43 IGHD2-8 IGHJ4 ggattcacatttgctgaatatacc atgcactgggtccgtcaagttccggggaagggtccggagtgggtctctctt attagttgggatggtggcagcaca tactatgcagactctgtgaagggccgattcaccatctccagagacaacagcaaggactccctgtatctgcaaatgaacagtctgagaactgaggacaccgccttgtattactgt gcaaaagataggattgggtccttgtactactttgaccac IGA1 8 213 5 3 1 2 4 6 1 2 3 2 JY8QFUQ01A72BF 16222 productive (see comment) Homsap IGHV4-61*01 F 983 94.12 208/221 nt 93.67 207/221 nt Homsap IGHJ5*02 F (see comment) 165 80.39 41/51 nt Homsap IGHD1-26*01 F 3 10 7 15 10.7.15 [1.17.38.11] CARGGSGGAYWNLIRDW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR3-IMGT at codon 67 of V-REGION: 'TATAATCCCCTG' (12 nucleotides at position 111 to 122 in the user submitted sequence), (do not cause frameshift); tctctggtggctccgtcagcagagatatttactactggagctggatccggcagcccccagggaagggactggagtggattgggtatatgtatcacagagggaacactaagTATAATCCCCTGtataatccctccctcgagagtcgagtcaccatttcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgtgcgagaggtgggtccggtggggcctactggaatttgatccgggactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [309] t102>a,S34>R(- - -)|g104>a,G35>D(- - -)|g107>t,S36>I(- - -)|c168>g,I56>M(+ + -)|t172>c,Y58>H(+ - -)|t177>a,S59>R(- - -)|g191>a,S64>N(- - -)|c195>t|c198>g,N66>K(+ - -)|c201>t|c204>t|a214>g,K72>E(+ - -)|a234>t| t102>a,S34>R(- - -)|g104>a,G35>D(- - -)|g107>t,S36>I(- - -)| c168>g,I56>M(+ + -)|t172>c,Y58>H(+ - -)|t177>a,S59>R(- - -)|g191>a,S64>N(- - -)|c195>t| c198>g,N66>K(+ - -)|c201>t|c204>t|a214>g,K72>E(+ - -)|a234>t| a320>g| 319 (320) 228 (229) 215 13 (14) 4 (5) 9 1 (2) 2 3 1 0 0 1 2 0 2 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 0 1 0 0 0 0 0 1 0 0 1 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 16 5 1 4 0 1 1 1 0 0 0 1 0 1 0 0 117 114 109 5 3 2 1 0 2 0 0 0 1 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcagcagagatatttactac tggagctggatccggcagcccccagggaagggactggagtggattgggtat atgtatcacagagggaacact aagtataatccctccctcgagagtcgagtcaccatttcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgt gcgagaggtgggtccggtggggcctactggaatttgatccgggac ARGGSGGAYWNLIRD IGHV4-61 IGHD1-26 IGHJ5 ggtggctccgtcagcagagatatttactac tggagctggatccggcagcccccagggaagggactggagtggattgggtat atgtatcacagagggaacact aagtataatccctccctcgagagtcgagtcaccatttcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgt gcgagaggtgggtccggtggggcctactggaatttgatccgggac IGA1 13 216 7 6 5 8 2 5 3 1 2 7 JY8QFUQ01A72FV 10563 productive Homsap IGHV3-11*01 F 948 93.93 201/214 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 183 84.31 43/51 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [0.17.38.11] CARDPHRGDGCTYDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) tggattcaccttcagtgactaccacatgggctggatccgccaggctccagggaaggggctggagtgcgtttcatacattagcactagtggtcgtgacatatacaacgcagactctgtgaagggccgattcaccatctccagggacaacgcccagaagtcactgtatctgcaaatgaacagcctgagagccgaggacacagccgtgtatttctgtgcgagggatccccatcgtggagatggctgcacttacgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [287] t112>c,Y38>H(+ - -)|a118>g,S40>G(+ + -)|g156>c,W52>C(+ - -)|t171>c|g173>c,S58>T(+ - +)|a187>c,S63>R(- - -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|t199>a,Y67>N(- - -)|a250>c,K84>Q(+ - -)|c255>g,N85>K(+ - -)|g297>a|a308>t,Y103>F(- + -)|a318>g| t112>c,Y38>H(+ - -)| a118>g,S40>G(+ + -)|g156>c,W52>C(+ - -)| t171>c|g173>c,S58>T(+ - +)|a187>c,S63>R(- - -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)| t199>a,Y67>N(- - -)|a250>c,K84>Q(+ - -)|c255>g,N85>K(+ - -)|g297>a|a308>t,Y103>F(- + -)| a318>g| 320 222 208 14 3 11 3 1 0 2 2 1 1 1 2 1 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 51 51 49 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 30 24 19 5 1 4 1 0 0 1 1 1 0 0 1 0 0 0 117 114 109 5 1 4 0 1 0 0 1 0 1 1 0 1 0 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t ggattcaccttcagtgactaccac atgggctggatccgccaggctccagggaaggggctggagtgcgtttcatac attagcactagtggtcgtgacata tacaacgcagactctgtgaagggccgattcaccatctccagggacaacgcccagaagtcactgtatctgcaaatgaacagcctgagagccgaggacacagccgtgtatttctgt gcgagggatccccatcgtggagatggctgcacttacgactcc ARDPHRGDGCTYDS IGHV3-11 IGHD5-24 IGHJ5 ggattcaccttcagtgactaccac atgggctggatccgccaggctccagggaaggggctggagtgcgtttcatac attagcactagtggtcgtgacata tacaacgcagactctgtgaagggccgattcaccatctccagggacaacgcccagaagtcactgtatctgcaaatgaacagcctgagagccgaggacacagccgtgtatttctgt gcgagggatccccatcgtggagatggctgcacttacgactcc IGA1 13 213 5 8 1 5 4 8 1 1 6 5 JY8QFUQ01A79R5 13031 productive Homsap IGHV3-9*01 F 981 94.93 206/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-6*01 F 1 8 8 15 8.8.15 [1.17.38.11] CAKSGTYSSSSGHFDLW in-frame + ctctggattcaactttaataattatgccatgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagtattagttggaataatggtaacacagactatgcgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgagaactgaggacacggccttatattactgtgcgaaatccgggacgtatagcagctcgtccggccactttgacctctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [294] c86>a,T29>N(- + -)|g103>a,D35>N(+ + -)|g106>a,D36>N(+ + -)|g163>a,G55>S(+ + -)|g185>a,S62>N(- - -)|g191>a,S64>N(- - -)|t194>c,I65>T(- - -)|g197>a,G66>D(- - -)|a206>g,D69>G(- - -)|g286>a,A96>T(- - -)|g303>a|a315>g| c86>a,T29>N(- + -)|g103>a,D35>N(+ + -)|g106>a,D36>N(+ + -)| g163>a,G55>S(+ + -)| g185>a,S62>N(- - -)|g191>a,S64>N(- - -)|t194>c,I65>T(- - -)| g197>a,G66>D(- - -)|a206>g,D69>G(- - -)|g286>a,A96>T(- - -)|g303>a| a315>g|g319>t,D107>S(- - -)|a320>c,D107>S(- - -)|t321>c,D107>S(- - -)|a322>g| 318 (322) 223 (227) 211 12 (16) 2 (3) 10 (13) 2 (3) 8 0 1 (2) 0 (1) 1 0 0 0 0 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 2 0 0 0 1 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 2 0 1 0 0 0 0 0 0 0 0 117 114 110 4 1 3 1 3 0 0 0 0 0 0 0 0 0 0 6 (10) 6 5 1 (5) 1 (2) 0 (3) 1 (2) 0 0 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaactttaataattatgcc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt attagttggaataatggtaacaca gactatgcgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgagaactgaggacacggccttatattactgt gcgaaatccgggacgtatagcagctcgtccggccactttgacctc AKSGTYSSSSGHFDL IGHV3-9 IGHD6-6 IGHJ4 ggattcaactttaataattatgcc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt attagttggaataatggtaacaca gactatgcgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgagaactgaggacacggccttatattactgt gcgaaatccgggacgtatagcagctcgtccggccactttgacctc IGG2 11 213 10 1 8 9 2 2 1 0 4 6 JY8QFUQ01A7AMF 9534 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 1025 97.67 210/215 nt NA Homsap IGHJ4*02 F (see comment) 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 7 13 8.7.13 [1.17.38.11] CARGGGYYFVAIDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) tctctggtggctccatcagtagttactactggagctggatccggcagcccccagggaagggactggagtggattgggtatatctataaaagtgggagcaccaactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaagcagttctccctgaaactgacctctgtgaccgctgcggacacggccgtgtattactgtgcgagaggtggtggttattacttcgtcgcgattgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtcagggcttcttccc NA IGA1 100 100 [285] t172>a,Y58>K(- - -)|c174>a,Y58>K(- - -)|c255>g,N85>K(+ - -)|g270>a|g275>c,S92>T(+ - +)| t172>a,Y58>K(- - -)|c174>a,Y58>K(- - -)| c255>g,N85>K(+ - -)|g270>a|g275>c,S92>T(+ - +)| a320>g| 319 (320) 222 (223) 217 5 (6) 1 (2) 4 0 (1) 1 0 0 0 1 0 1 1 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 19 2 0 2 0 0 0 0 0 1 0 1 0 0 0 0 117 114 111 3 1 2 0 1 0 0 0 0 0 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagtagttactac tggagctggatccggcagcccccagggaagggactggagtggattgggtat atctataaaagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaagcagttctccctgaaactgacctctgtgaccgctgcggacacggccgtgtattactgt gcgagaggtggtggttattacttcgtcgcgattgactcc ARGGGYYFVAIDS IGHV4-59 IGHD3-22 IGHJ4 ggtggctccatcagtagttactac tggagctggatccggcagcccccagggaagggactggagtggattgggtat atctataaaagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaagcagttctccctgaaactgacctctgtgaccgctgcggacacggccgtgtattactgt gcgagaggtggtggttattacttcgtcgcgattgactcc IGA1 5 210 1 4 1 4 0 1 1 0 2 2 JY8QFUQ01A7C1U 14942 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 959 93.58 204/218 nt 93.12 203/218 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD6-13*01 F 3 8 8 11 8.8.11 [1.17.38.11] CVRDRSGQAVGYW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 74 of V-REGION: 'G' (1 nucleotides at position 126 to 126 in the user submitted sequence), (cause frameshift); cctctggattcaccttcagtagttactggatgcactgggtccgccaagttccagggaagggactggtgtgggtctcacgaattaatactgatgggagtgccacaagttacgcggactccgtgaggGggccgattcaccatctccagagacaacgccaagaacacgctatatcttcaaatgaacagtctgagagtcgaagacacggctgtctattactgtgtaagagatcggtccggtcaggcggtaggatactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [284] c108>t|c134>t,A45>V(+ - +)|g147>a|t165>a|g173>c,S58>T(+ - +)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|c198>t|a215>g,K72>R(+ + +)|g261>a|g267>t|c287>t,A96>V(+ - +)|g291>a|g303>c|c314>t,A105>V(+ - +)| c108>t| c134>t,A45>V(+ - +)|g147>a|t165>a| g173>c,S58>T(+ - +)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| c198>t|a215>g,K72>R(+ + +)|g261>a|g267>t|c287>t,A96>V(+ - +)|g291>a|g303>c| c314>t,A105>V(+ - +)| 320 226 211 15 8 7 2 3 5 0 0 0 0 1 3 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 1 0 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 2 0 0 0 117 114 107 7 5 2 1 2 2 0 0 0 0 0 1 0 1 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtagttactgg atgcactgggtccgccaagttccagggaagggactggtgtgggtctcacga attaatactgatgggagtgccaca agttacgcggactccgtgaggggccgattcaccatctccagagacaacgccaagaacacgctatatcttcaaatgaacagtctgagagtcgaagacacggctgtctattactgt gtaagagatcggtccggtcaggcggtaggatac VRDRSGQAVGY IGHV3-74 IGHD6-13 IGHJ4 ggattcaccttcagtagttactgg atgcactgggtccgccaagttccagggaagggactggtgtgggtctcacga attaatactgatgggagtgccaca agttacgcggactccgtgaggggccgattcaccatctccagagacaacgccaagaacacgctatatcttcaaatgaacagtctgagagtcgaagacacggctgtctattactgt gtaagagatcggtccggtcaggcggtaggatac IGG1 14 213 9 5 7 11 2 3 7 1 3 3 JY8QFUQ01A7C84 4269 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 940 93.06 201/216 nt 91.67 198/216 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [1.16.38.11] CVRAGFGELSGFDCW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 54 of V-REGION: 'CAG' (3 nucleotides at position 73 to 75 in the user submitted sequence), (do not cause frameshift);in CDR2-IMGT at codon 56 of V-REGION: 'T' (1 nucleotides at position 82 to 82 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 46 of V-REGION: 1 nucleotides (from position 52 in the user submitted sequence), (cause frameshift) ctctggattcacctttggttattatggcatgagctgggtccgccaagctccggggaggggctgggatgggtcCAGtctggtTattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [291] a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a142>g,K48>E(+ - -)|a152>g,E51>G(- - -)|g153>a,E51>G(- - -)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a142>g,K48>E(+ - -)|a152>g,E51>G(- - -)|g153>a,E51>G(- - -)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 223 (224) 207 16 (17) 2 (3) 14 3 4 1 1 1 (2) 0 2 0 2 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 50 47 3 0 3 2 1 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ctct ggattcacctttggttattatggc atgagctgggtccgccaagctccggggaggggctgggatgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgagctgggtccgccaagctccggggaggggctgggatgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 15 212 8 7 4 8 4 7 2 0 9 4 JY8QFUQ01A7G35 12836 productive (see comment) Homsap IGHV1-8*01 F 822 87.38 187/214 nt 86.92 186/214 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 2 8 8 12 8.8.12 [0.17.38.11] CARGRGYGSYADHW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 57 of V-REGION: 'G' (1 nucleotides at position 82 to 82 in the user submitted sequence), (cause frameshift); tggatacagcttcagcaattatgatatcaactgggtgcggcaggccactggacaagggcttcagtggatggggtggatgaaGcccccacagtgccaatatcgtctacgcgcagaaatttcagggcagagtcaccatgaccagtgacgcctccataaccacagcctacatggaactgagcaacctgaaatctgacgacacggccgtgtattattgtgcgaggggccgagggtacggaagttacgctgaccactggggccagggaaccccggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [282] c86>g,T29>S(+ - +)|c104>g,T35>S(+ - +)|g107>a,S36>N(- - -)|a129>g|g151>c,E51>Q(+ + -)|a162>g|t174>c|a175>c,N59>H(- - -)|g188>c,G63>A(- + -)|t189>c,G63>A(- + -)|c192>t|c194>t,T65>I(- - -)|a195>c,T65>I(- - -)|g197>t,G66>V(- - -)|t201>c|a204>g|g210>a|c213>t|g240>t,R80>S(- - -)|a241>g,N81>D(+ + -)|a244>g,T82>A(- - -)|g254>c,S85>T(+ - +)|g270>a|g278>a,S93>N(- - -)|g284>a,R95>K(+ + +)|g291>c,E97>D(+ - +)|c309>t|a318>g| c86>g,T29>S(+ - +)|c104>g,T35>S(+ - +)|g107>a,S36>N(- - -)| a129>g|g151>c,E51>Q(+ + -)|a162>g| t174>c|a175>c,N59>H(- - -)|g188>c,G63>A(- + -)|t189>c,G63>A(- + -)|c192>t|c194>t,T65>I(- - -)|a195>c,T65>I(- - -)| g197>t,G66>V(- - -)|t201>c|a204>g|g210>a|c213>t|g240>t,R80>S(- - -)|a241>g,N81>D(+ + -)|a244>g,T82>A(- - -)|g254>c,S85>T(+ - +)|g270>a|g278>a,S93>N(- - -)|g284>a,R95>K(+ + +)|g291>c,E97>D(+ - +)|c309>t| a318>g| 320 222 194 28 11 17 6 5 4 3 2 0 0 0 4 2 2 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 0 2 0 0 51 51 48 3 2 1 2 0 0 0 0 0 0 0 1 0 0 0 30 24 17 7 2 5 0 0 2 2 2 0 0 0 1 0 0 0 117 114 100 14 6 8 3 4 2 1 0 0 0 0 2 0 2 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,118-119(FR2)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|aa,241-242(FR3)|ta,251-252(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,251-252(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|agtt,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,118-121(FR2)|aacc,169-172(CDR2)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacagcttcagcaattatgat atcaactgggtgcggcaggccactggacaagggcttcagtggatggggtgg atgaacccccacagtgccaatatc gtctacgcgcagaaatttcagggcagagtcaccatgaccagtgacgcctccataaccacagcctacatggaactgagcaacctgaaatctgacgacacggccgtgtattattgt gcgaggggccgagggtacggaagttacgctgaccac ARGRGYGSYADH IGHV1-8 IGHD3-10 IGHJ4 ggatacagcttcagcaattatgat atcaactgggtgcggcaggccactggacaagggcttcagtggatggggtgg atgaacccccacagtgccaatatc gtctacgcgcagaaatttcagggcagagtcaccatgaccagtgacgcctccataaccacagcctacatggaactgagcaacctgaaatctgacgacacggccgtgtattattgt gcgaggggccgagggtacggaagttacgctgaccac IGA1 27 213 17 10 9 17 8 10 8 2 9 8 JY8QFUQ01A7GLV 4917 productive Homsap IGHV4-61*02 F 821 85.97 190/221 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F 201 88.24 45/51 nt Homsap IGHD3-10*01 F 2 10 7 18 10.7.18 [1.17.38.11] CAREDYYGSGSYGRAWFDSW in-frame + tctctggaggctccatcagcagtggaagttactactggacctggatccggcagcccgccgggaagactctggagtggattgggcgcttctacagtcgtgggggtgtcgactacaacccctccctcaggggtcgagtcaccatttcagcggacacgtccaagagccagttctcccttaatctgacttctgcgaccgccactgacacggccgtgtatttctgtgcgagagaggattactatggttcggggagctatgggcgggcctggttcgactcctggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtagtcatcgcctgcctggtccagggcttcttccc NA IGA1 93 89 [306] t81>a|t105>a|g119>c,S40>T(+ - +)|g145>a,G49>T(+ - -)|g146>c,G49>T(+ - -)|a147>t,G49>T(+ - -)|t165>c|a166>t,I56>F(+ - -)|t171>c|c173>g,T58>S(+ - +)|c174>t,T58>S(+ - +)|a175>c,S59>R(- - -)|a190>g,S64>G(+ + -)|c192>t,S64>G(+ + -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|a196>g,N66>D(+ + -)|a215>g,K72>R(+ + +)|a220>g,S74>G(+ + -)|a234>t|t239>c,V80>A(+ - +)|a240>g,V80>A(+ - +)|a254>g,N85>S(- - -)|g267>t|g270>t,K90>N(+ - -)|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|t281>c,V94>A(+ - +)|g289>a,A97>T(- - -)|a291>t,A97>T(- - -)|a308>t,Y103>F(- + -)| t81>a|t105>a| g119>c,S40>T(+ - +)|g145>a,G49>T(+ - -)|g146>c,G49>T(+ - -)|a147>t,G49>T(+ - -)|t165>c| a166>t,I56>F(+ - -)|t171>c|c173>g,T58>S(+ - +)|c174>t,T58>S(+ - +)|a175>c,S59>R(- - -)|a190>g,S64>G(+ + -)|c192>t,S64>G(+ + -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)| a196>g,N66>D(+ + -)|a215>g,K72>R(+ + +)|a220>g,S74>G(+ + -)|a234>t|t239>c,V80>A(+ - +)|a240>g,V80>A(+ - +)|a254>g,N85>S(- - -)|g267>t|g270>t,K90>N(+ - -)|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|t281>c,V94>A(+ - +)|g289>a,A97>T(- - -)|a291>t,A97>T(- - -)|a308>t,Y103>F(- + -)| 320 229 198 31 6 25 7 2 4 4 1 0 5 2 3 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 2 0 0 0 0 0 0 0 0 2 0 0 0 0 51 51 46 5 1 4 0 1 0 1 0 0 1 0 2 0 0 0 30 21 12 9 1 8 2 0 3 1 1 0 1 0 0 1 0 0 117 114 99 15 2 13 5 1 1 2 0 0 3 0 1 0 2 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggaggctccatcagcagtggaagttactac tggacctggatccggcagcccgccgggaagactctggagtggattgggcgc ttctacagtcgtgggggtgtc gactacaacccctccctcaggggtcgagtcaccatttcagcggacacgtccaagagccagttctcccttaatctgacttctgcgaccgccactgacacggccgtgtatttctgt gcgagagaggattactatggttcggggagctatgggcgggcctggttcgactcc AREDYYGSGSYGRAWFDS IGHV4-61 IGHD3-10 IGHJ5 ggaggctccatcagcagtggaagttactac tggacctggatccggcagcccgccgggaagactctggagtggattgggcgc ttctacagtcgtgggggtgtc gactacaacccctccctcaggggtcgagtcaccatttcagcggacacgtccaagagccagttctcccttaatctgacttctgcgaccgccactgacacggccgtgtatttctgt gcgagagaggattactatggttcggggagctatgggcgggcctggttcgactcc IGA1 31 216 17 14 6 12 11 19 3 3 17 8 JY8QFUQ01A7HLN 2697 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 887 89.91 196/218 nt NA Homsap IGHJ4*02 F 114 70.83 34/48 nt Homsap IGHD6-19*01 F 1 8 8 10 8.8.10 [1.17.38.11] CMNELGYCSGGG in-frame + cctctggattcacttttagcagccatatgatgagttgggtccgccaggctccagggaaggggctggaatgggtctcaactattcgtgccagtggtgataggacacactatgcagactccgtgaggggccgcttcaccatctccagagacaattccaagaacacgctgcatttgcaaatgtacagcctgagagtcgaggacacggccgtatactactgtatgaacgaactcggatattgtagtggtgggggggaccagggagccctggtctccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [279] c87>t|t109>c,Y37>H(+ - -)|g112>a,A38>M(+ - -)|c113>t,A38>M(+ - -)|c114>g,A38>M(+ - -)|c120>t|g153>a|g163>a,A55>T(- - -)|a169>c,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g188>a,G63>D(- - -)|c192>g,S64>R(- - -)|t196>c,Y66>H(+ - -)|c201>t|a215>g,K72>R(+ + +)|g225>c|t262>c,Y88>H(+ - -)|c265>t|a274>t,N92>Y(- - -)|c287>t,A96>V(+ - +)|t306>c|g313>a,A105>M(+ - -)|c314>t,A105>M(+ - -)| c87>t|t109>c,Y37>H(+ - -)|g112>a,A38>M(+ - -)|c113>t,A38>M(+ - -)|c114>g,A38>M(+ - -)| c120>t|g153>a|g163>a,A55>T(- - -)| a169>c,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g188>a,G63>D(- - -)|c192>g,S64>R(- - -)| t196>c,Y66>H(+ - -)|c201>t|a215>g,K72>R(+ + +)|g225>c|t262>c,Y88>H(+ - -)|c265>t|a274>t,N92>Y(- - -)|c287>t,A96>V(+ - +)|t306>c| g313>a,A105>M(+ - -)|c314>t,A105>M(+ - -)|a318>c,K106>N(+ - -)| 320 226 201 25 7 18 1 5 7 5 2 0 1 0 2 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 2 1 0 0 0 0 0 1 0 0 51 51 48 3 2 1 0 2 1 0 0 0 0 0 0 0 0 0 30 24 19 5 0 5 0 1 0 1 1 0 0 0 1 1 0 0 117 114 105 9 4 5 1 0 3 3 0 0 1 0 1 0 0 0 8 8 5 3 0 3 0 1 1 0 1 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacttttagcagccatatg atgagttgggtccgccaggctccagggaaggggctggaatgggtctcaact attcgtgccagtggtgataggaca cactatgcagactccgtgaggggccgcttcaccatctccagagacaattccaagaacacgctgcatttgcaaatgtacagcctgagagtcgaggacacggccgtatactactgt atgaacgaactcggatattgtagtggtggg MNELGYCSGG IGHV3-23 IGHD6-19 IGHJ4 ggattcacttttagcagccatatg atgagttgggtccgccaggctccagggaaggggctggaatgggtctcaact attcgtgccagtggtgataggaca cactatgcagactccgtgaggggccgcttcaccatctccagagacaattccaagaacacgctgcatttgcaaatgtacagcctgagagtcgaggacacggccgtatactactgt atgaacgaactcggatattgtagtggtggg IGA1 22 213 16 6 10 14 6 8 6 1 6 9 JY8QFUQ01A7KJZ 2639 productive (see comment) Homsap IGHV3-7*01 F 870 88.64 195/220 nt 88.18 194/220 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-10*01 F 2 8 8 16 8.8.16 [2.17.38.11] CARDPYKPGSGNPGFDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'G' (1 nucleotides at position 11 to 11 in the user submitted sequence), (cause frameshift); agcctctggaGttcacctttagtgactatttgatgacttgggtccgccaggctccaggaaagggactggagtgggtggccaacataaaagaagatggacttgctacattctatgtggactctgtgagggaccgattcaccatctccagagacaacgccaagaactcactttatttgcaaatgaattacctgagagtcgaggacacggctgtttattattgtgcgagggatccctataaacctggttcgggcaatccgggctttgactactggggccagggagcgcaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [300] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g113>t,W38>L(+ - -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g141>a|g147>a|g171>a|c172>g,Q58>E(+ + -)|a187>c,S63>L(- - -)|g188>t,S63>L(- - -)|a191>c,E64>A(- - -)|g192>t,E64>A(- - -)|a194>c,K65>T(- - -)|a197>t,Y66>F(- + -)|a215>g,K72>R(+ + +)|g221>a,G74>D(- - -)|g261>t|c265>t|c276>t|a277>t,S93>Y(+ - -)|g278>a,S93>Y(+ - -)|c287>t,A96>V(+ - +)|g303>t|c309>t|a318>g| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g113>t,W38>L(+ - -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g141>a|g147>a| g171>a|c172>g,Q58>E(+ + -)|a187>c,S63>L(- - -)|g188>t,S63>L(- - -)|a191>c,E64>A(- - -)|g192>t,E64>A(- - -)|a194>c,K65>T(- - -)| a197>t,Y66>F(- + -)|a215>g,K72>R(+ + +)|g221>a,G74>D(- - -)|g261>t|c265>t|c276>t|a277>t,S93>Y(+ - -)|g278>a,S93>Y(+ - -)|c287>t,A96>V(+ - +)|g303>t|c309>t| a318>g| 320 228 202 26 9 17 3 6 5 0 3 0 2 0 1 1 5 0 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 1 0 0 0 0 0 0 0 0 1 0 51 51 47 4 2 2 0 2 1 0 0 0 0 0 1 0 0 0 30 24 17 7 1 6 0 1 0 0 3 0 0 0 0 1 2 0 117 114 103 11 5 6 1 2 4 0 0 0 2 0 0 0 2 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct ggattcacctttagtgactatttg atgacttgggtccgccaggctccaggaaagggactggagtgggtggccaac ataaaagaagatggacttgctaca ttctatgtggactctgtgagggaccgattcaccatctccagagacaacgccaagaactcactttatttgcaaatgaattacctgagagtcgaggacacggctgtttattattgt gcgagggatccctataaacctggttcgggcaatccgggctttgactac ARDPYKPGSGNPGFDY IGHV3-7 IGHD3-10 IGHJ4 ggattcacctttagtgactatttg atgacttgggtccgccaggctccaggaaagggactggagtgggtggccaac ataaaagaagatggacttgctaca ttctatgtggactctgtgagggaccgattcaccatctccagagacaacgccaagaactcactttatttgcaaatgaattacctgagagtcgaggacacggctgtttattattgt gcgagggatccctataaacctggttcgggcaatccgggctttgactac IGA1 25 213 13 12 11 18 2 7 7 1 8 9 JY8QFUQ01A7LQC 21562 productive Homsap IGHV4-59*02 F 962 94.42 203/215 nt NA Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD3-10*01 F 3 8 7 17 8.7.17 [1.17.38.11] CARHSMGVTIVRGHWFDPW in-frame + tctctggtggctccgtcagcagttattactggagctggatccgacagcccccagggaagggactggagtggattggctatatccatgacagtgggagcagcaactacaacccctccctcaagagtcgagtcaccatatctgtggacacgtccaagaaccagttctccctgaagctgacttctgtgaccgctgcggacacggccgtatattactgtgcgagacattctatgggtgttactatagttcggggacactggttcgacccctggggccagggaaccctggtcaccgtctcatcagcctccaccaagggcccatcggtcttcccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtcggaacctc NA IGG1 100 94 [297, 298] t105>c|c111>t|g129>a|g162>c|t169>c,Y57>H(+ - -)|t172>g,Y58>D(- - -)|c194>g,T65>S(+ - +)|a237>t|a240>g|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g303>a| t105>c|c111>t| g129>a|g162>c| t169>c,Y57>H(+ - -)|t172>g,Y58>D(- - -)|c194>g,T65>S(+ - +)| a237>t|a240>g|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g303>a| g319>c| 318 (320) 221 (223) 209 (210) 12 (13) 7 (8) 5 1 2 2 2 0 0 1 0 2 (3) 1 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 2 0 0 0 1 1 0 0 0 0 0 0 0 0 51 51 49 2 2 0 0 1 0 0 0 0 0 0 1 0 0 0 30 21 18 3 0 3 0 0 0 1 0 0 0 0 0 1 0 1 117 114 109 5 3 2 1 1 1 0 0 0 1 0 1 0 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcagcagttattac tggagctggatccgacagcccccagggaagggactggagtggattggctat atccatgacagtgggagcagc aactacaacccctccctcaagagtcgagtcaccatatctgtggacacgtccaagaaccagttctccctgaagctgacttctgtgaccgctgcggacacggccgtatattactgt gcgagacattctatgggtgttactatagttcggggacactggttcgacccc ARHSMGVTIVRGHWFDP IGHV4-59 IGHD3-10 IGHJ5 ggtggctccgtcagcagttattac tggagctggatccgacagcccccagggaagggactggagtggattggctat atccatgacagtgggagcagc aactacaacccctccctcaagagtcgagtcaccatatctgtggacacgtccaagaaccagttctccctgaagctgacttctgtgaccgctgcggacacggccgtatattactgt gcgagacattctatgggtgttactatagttcggggacactggttcgacccc IGG1 12 210 7 5 4 7 3 5 5 2 2 3 JY8QFUQ01A7MBK 12244 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 933 91.82 202/220 nt 91.36 201/220 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-3*01 F 2 8 8 10 8.8.10 [2.17.38.11] CTKDTFGREDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 38 of V-REGION: 'G' (1 nucleotides at position 30 to 30 in the user submitted sequence), (cause frameshift); agcctctggattcaccttcagtagatactGggatgcactgggtccgccaggctccagggaaggggctggagtgggtcgcacgtactaatgaagatgggagtactaaaaactacgcggactccgtgaagggccgattcaccatcttcagagacaacaccaagaacacactatatctgcaaatgaacagtctgagagacgaggacacggctgtgtattattgtacaaaagacacgtttgggagagaagactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [282] c108>a,S36>R(- - -)|a132>g|t152>a,V51>E(- + -)|t160>g,S54>A(- + -)|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|c194>a,T65>K(- - -)|g197>a,S66>N(- - -)|c236>t,S79>F(- - -)|g247>a,A83>T(- - -)|g258>a|g261>a|c287>a,A96>D(- - -)|c309>t|g313>a,A105>T(- - -)|g317>a,R106>K(+ + +)| c108>a,S36>R(- - -)| a132>g|t152>a,V51>E(- + -)|t160>g,S54>A(- + -)| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|c194>a,T65>K(- - -)| g197>a,S66>N(- - -)|c236>t,S79>F(- - -)|g247>a,A83>T(- - -)|g258>a|g261>a|c287>a,A96>D(- - -)|c309>t| g313>a,A105>T(- - -)|g317>a,R106>K(+ + +)| 320 228 208 20 4 16 2 7 3 1 0 3 0 2 1 0 0 1 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 48 3 1 2 1 0 0 0 0 0 0 1 0 0 0 1 30 24 17 7 0 7 1 1 1 1 0 1 0 1 1 0 0 0 117 114 107 7 3 4 0 4 2 0 0 1 0 0 0 0 0 0 8 8 6 2 0 2 0 2 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct ggattcaccttcagtagatactgg atgcactgggtccgccaggctccagggaaggggctggagtgggtcgcacgt actaatgaagatgggagtactaaa aactacgcggactccgtgaagggccgattcaccatcttcagagacaacaccaagaacacactatatctgcaaatgaacagtctgagagacgaggacacggctgtgtattattgt acaaaagacacgtttgggagagaagactac TKDTFGREDY IGHV3-74 IGHD3-3 IGHJ4 ggattcaccttcagtagatactgg atgcactgggtccgccaggctccagggaaggggctggagtgggtcgcacgt actaatgaagatgggagtactaaa aactacgcggactccgtgaagggccgattcaccatcttcagagacaacaccaagaacacactatatctgcaaatgaacagtctgagagacgaggacacggctgtgtattattgt acaaaagacacgtttgggagagaagactac IGA1 18 213 11 7 8 12 3 6 4 0 6 8 JY8QFUQ01A7MWW 3030 productive Homsap IGHV3-9*01 F 873 89.4 194/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [1.17.38.11] CVKDRKGSNWYYFENW in-frame + ctctggattcaggtttgatgattatgccatgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagtatcagctggggtggtgctagtatcctctatgcggactccgtgaagggccgattcaccatctccagagacaatgccaggaactccctctacttgcaaatggacagtctgagacctgatgacacggccttctattactgtgtaaaagatcgaaagggcagcaattggtactactttgagaactggggccagggaaccctggtctccgtctcctcagcatcccctgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgctgcctggtccagggcttcttccccca NA IGA1 79 79 [291] c86>g,T29>R(- - -)|c87>g,T29>R(- - -)|g163>a,G55>S(+ + -)|t168>c|t171>c|a175>g,N59>G(- - -)|a176>g,N59>G(- - -)|a184>g,S62>G(+ + -)|g188>c,G63>A(- + -)|c192>t|a195>c|g196>c,G66>L(- - -)|g197>t,G66>L(- - -)|t210>c|c246>t|a251>g,K84>R(+ + +)|g261>c|t264>c|c265>t|a274>g,N92>D(+ + -)|g286>c,A96>P(- - -)|g291>t,E97>D(+ - +)|g303>c,L101>F(+ - -)|c314>t,A105>V(+ - +)| c86>g,T29>R(- - -)|c87>g,T29>R(- - -)| g163>a,G55>S(+ + -)| t168>c|t171>c|a175>g,N59>G(- - -)|a176>g,N59>G(- - -)|a184>g,S62>G(+ + -)|g188>c,G63>A(- + -)|c192>t|a195>c| g196>c,G66>L(- - -)|g197>t,G66>L(- - -)|t210>c|c246>t|a251>g,K84>R(+ + +)|g261>c|t264>c|c265>t|a274>g,N92>D(+ + -)|g286>c,A96>P(- - -)|g291>t,E97>D(+ - +)|g303>c,L101>F(+ - -)| c314>t,A105>V(+ - +)|a322>c| 321 (322) 226 (227) 202 24 (25) 9 (10) 15 5 1 4 4 1 (2) 0 0 0 5 2 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 0 0 0 2 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 16 8 4 4 3 0 1 2 1 0 0 0 1 0 0 0 117 114 102 12 5 7 2 0 2 2 0 0 0 0 4 0 2 0 9 (10) 9 8 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaggtttgatgattatgcc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt atcagctggggtggtgctagtatc ctctatgcggactccgtgaagggccgattcaccatctccagagacaatgccaggaactccctctacttgcaaatggacagtctgagacctgatgacacggccttctattactgt gtaaaagatcgaaagggcagcaattggtactactttgagaac VKDRKGSNWYYFEN IGHV3-9 IGHD5-24 IGHJ4 ggattcaggtttgatgattatgcc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt atcagctggggtggtgctagtatc ctctatgcggactccgtgaagggccgattcaccatctccagagacaatgccaggaactccctctacttgcaaatggacagtctgagacctgatgacacggccttctattactgt gtaaaagatcgaaagggcagcaattggtactactttgagaac IGA1 23 213 13 10 4 13 9 10 5 4 8 6 JY8QFUQ01A7NVO 12498 productive (see comment) Homsap IGHV1-3*01 F 912 92.06 197/214 nt 91.12 195/214 nt Homsap IGHJ4*02 F (see comment) 231 97.92 47/48 nt Homsap IGHD1-26*01 F 2 8 8 14 8.8.14 [0.17.38.11] CARDGEPLGGDYFDSW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 55 to 55 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 80 of V-REGION: 'G' (1 nucleotides at position 142 to 142 in the user submitted sequence), (cause frameshift); tggatacaccttcacttcttatgctgtaaactgggtgcgccaggcccccggacaAaaggcttgagtggatgggatggatcaacgctggcacgggtaacacaaaatattcacagaagtttcagggcagagtcaccattaccaGgggacacatccgcggacacagtcttcatggagctgagcagtctaagatctgaagacacggctgtgtattattgtgcgagagatggggagccactgggaggcgactactttgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [289] a106>t|g107>c|c108>t|a115>g,M39>V(+ - -)|g117>a,M39>V(+ - -)|c118>a,H40>N(- - -)|t120>c,H40>N(- - -)|a185>c,N62>T(- + -)|t186>g,N62>T(- + -)|c213>t|a253>g,S85>D(- - -)|g254>a,S85>D(- - -)|c260>t,A87>V(+ - +)|a263>t,Y88>F(- + -)|c279>t|g282>a|c309>t| a106>t|g107>c|c108>t| a115>g,M39>V(+ - -)|g117>a,M39>V(+ - -)|c118>a,H40>N(- - -)|t120>c,H40>N(- - -)| a185>c,N62>T(- + -)|t186>g,N62>T(- + -)| c213>t|a253>g,S85>D(- - -)|g254>a,S85>D(- - -)|c260>t,A87>V(+ - +)|a263>t,Y88>F(- + -)|c279>t|g282>a|c309>t| 320 222 205 17 7 10 2 3 5 1 1 1 2 0 1 0 0 1 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 3 0 0 0 1 0 0 0 1 0 1 0 0 0 51 51 47 4 0 4 1 1 0 1 0 1 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 1 0 0 0 0 0 0 1 117 114 106 8 4 4 1 2 4 0 0 0 1 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|ta,262-263(FR3)|aa,290-291(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,11-12(FR1)|tt,62-63(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,120-121(FR2)|tt,149-150(FR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,201-202(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcacttcttatgct gtaaactgggtgcgccaggcccccggacaaaggcttgagtggatgggatgg atcaacgctggcacgggtaacaca aaatattcacagaagtttcagggcagagtcaccattaccagggacacatccgcggacacagtcttcatggagctgagcagtctaagatctgaagacacggctgtgtattattgt gcgagagatggggagccactgggaggcgactactttgactcc ARDGEPLGGDYFDS IGHV1-3 IGHD1-26 IGHJ4 ggatacaccttcacttcttatgct gtaaactgggtgcgccaggcccccggacaaaggcttgagtggatgggatgg atcaacgctggcacgggtaacaca aaatattcacagaagtttcagggcagagtcaccattaccagggacacatccgcggacacagtcttcatggagctgagcagtctaagatctgaagacacggctgtgtattattgt gcgagagatggggagccactgggaggcgactactttgactcc IGA1 17 213 11 6 8 10 3 7 4 3 8 2 JY8QFUQ01A7QC0 9353 productive (see comment) Homsap IGHV3-33*05 F (see comment) 752 83.03 181/218 nt 82.11 179/218 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-12*01 F 3 8 8 13 8.8.13 [2.16.38.11] CVKGRGYNDYGLDYW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (83,03% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR2-IMGT at codon 62 of V-REGION: 'A' (1 nucleotides at position 93 to 93 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 60 in the user submitted sequence), (cause frameshift) gcgtctggattctccttcagcaattatgccatccactgggtccgccaggctccaggcaagggctggagtgggtggcgaccatttcatatgatAttaatgaaaagatattatgcagagtccgtgaggggccgattcaccctctccagagacaattccaagaacactctcgatctgctcatggatacccttcggttcgacgacacggctgtctattattgtgttaaaggacgtggatacaatgactacggcctcgactactggggcccgggaaccctgctcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [290] a85>t,T29>S(+ - +)|t105>c|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g113>c,G38>A(- + -)|g117>c,M39>I(+ + -)|a162>g|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)|t165>c,V55>T(- - -)|a168>t|g184>t,G62>L(- - -)|g185>t,G62>L(- - -)|g188>t,S63>M(- - -)|t189>g,S63>M(- - -)|t192>a,N64>K(+ - -)|a194>g,K65>R(+ + +)|c198>t|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|a232>c,I78>L(+ + +)|g258>t|g261>c|t262>g,Y88>D(- - -)|a269>t,Q90>L(- - -)|a270>c,Q90>L(- - -)|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|g278>c,S93>T(+ - +)|g282>t|a283>c|a285>g|g286>t,A96>F(+ - -)|c287>t,A96>F(+ - -)|g291>c,E97>D(+ - +)|g303>c|c309>t| a85>t,T29>S(+ - +)|t105>c|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g113>c,G38>A(- + -)| g117>c,M39>I(+ + -)|a162>g|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)|t165>c,V55>T(- - -)| a168>t|g184>t,G62>L(- - -)|g185>t,G62>L(- - -)|g188>t,S63>M(- - -)|t189>g,S63>M(- - -)|t192>a,N64>K(+ - -)|a194>g,K65>R(+ + +)| c198>t|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|a232>c,I78>L(+ + +)|g258>t|g261>c|t262>g,Y88>D(- - -)|a269>t,Q90>L(- - -)|a270>c,Q90>L(- - -)|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|g278>c,S93>T(+ - +)|g282>t|a283>c|a285>g|g286>t,A96>F(+ - -)|c287>t,A96>F(+ - -)|g291>c,E97>D(+ - +)|g303>c|c309>t| c314>t,A105>V(+ - +)|g315>t,A105>V(+ - +)|g317>a,R106>K(+ + +)|a320>g| 319 (320) 225 (226) 185 40 (41) 11 (12) 29 5 (6) 3 6 3 3 0 3 1 6 1 7 2 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 1 1 0 0 1 0 1 0 0 0 51 50 45 5 1 4 1 1 0 2 0 0 0 0 1 0 0 0 30 24 17 7 1 6 1 0 0 0 0 0 1 1 0 0 3 1 117 114 94 20 8 12 3 0 4 0 3 0 1 0 4 1 3 1 7 (8) 7 4 3 (4) 0 (1) 3 0 (1) 1 1 0 0 0 0 0 0 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcgtct ggattctccttcagcaattatgcc atccactgggtccgccaggctccaggcaagggctggagtgggtggcgacc atttcatatgatttaatgaaaaga tattatgcagagtccgtgaggggccgattcaccctctccagagacaattccaagaacactctcgatctgctcatggatacccttcggttcgacgacacggctgtctattattgt gttaaaggacgtggatacaatgactacggcctcgactac VKGRGYNDYGLDY IGHV3-33 IGHD5-12 IGHJ4 ggattctccttcagcaattatgcc atccactgggtccgccaggctccaggcaagggctggagtgggtggcgacc atttcatatgatttaatgaaaaga tattatgcagagtccgtgaggggccgattcaccctctccagagacaattccaagaacactctcgatctgctcatggatacccttcggttcgacgacacggctgtctattattgt gttaaaggacgtggatacaatgactacggcctcgactac IGG1 37 212 15 22 7 20 8 17 9 2 16 10 JY8QFUQ01A7S16 9907 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 1009 98.1 206/210 nt 96.67 203/210 nt Homsap IGHJ6*02 F 229 85.48 53/62 nt Homsap IGHD4-17*01 F 2 7 8 16 7.8.16 [X.17.38.11] CARQLDYGDYSRHGLDVW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'C' (1 nucleotides at position 49 to 49 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 53 of V-REGION: 'G' (1 nucleotides at position 67 to 67 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 65 of V-REGION: 'C' (1 nucleotides at position 97 to 97 in the user submitted sequence), (cause frameshift); cacaactttaccggctactggatcggctgggtgcgccagatgcccgggCaaaggcctggagtggatGggggatcatctatcctggtgactctgataCccagatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcagcaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccgtgtattactgtgcgagacaattagactacggtgactactctaggcacggtttggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [292] t82>c,Y28>H(+ - -)|g86>a,S29>N(- - -)|a106>g,S36>G(+ + -)|a301>g,M101>V(+ - -)| t82>c,Y28>H(+ - -)|g86>a,S29>N(- - -)|a106>g,S36>G(+ + -)| a301>g,M101>V(+ - -)| 320 218 214 4 0 4 2 1 0 1 0 0 0 0 0 0 0 0 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 21 18 3 0 3 1 1 0 1 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 113 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| cacaactttaccggctactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgatacc agatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcagcaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccgtgtattactgt gcgagacaattagactacggtgactactctaggcacggtttggacgtc ARQLDYGDYSRHGLDV IGHV5-51 IGHD4-17 IGHJ6 cacaactttaccggctactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgatacc agatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcagcaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccgtgtattactgt gcgagacaattagactacggtgactactctaggcacggtttggacgtc IGA2 4 210 4 0 1 1 3 3 0 0 1 3 JY8QFUQ01A7TR1 20726 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 882 89.86 195/217 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [1.17.38.11] CTRDIGGRYSYW in-frame + ctccggattcacctttagtagtttctggatgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacggactaatgagtatgggagtatcacaaactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgtacaagggacatcggtgggcgctactcctactggggccagggagtcctggtctccgtctcatcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [278] t78>c|c90>t|c108>t|a110>t,Y37>F(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g247>a,A83>T(- - -)|c257>g,T86>R(- - -)|g261>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|g313>a,A105>T(- - -)|a318>g| t78>c| c90>t|c108>t|a110>t,Y37>F(- + -)| c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g247>a,A83>T(- - -)|c257>g,T86>R(- - -)|g261>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| g313>a,A105>T(- - -)|a318>g| 320 225 201 24 7 17 4 7 3 2 0 0 1 0 0 1 3 3 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 2 0 0 0 1 0 0 0 0 0 51 51 46 5 2 3 1 1 1 0 0 0 0 0 0 0 0 2 30 24 18 6 0 6 1 1 0 1 0 0 0 0 0 0 2 1 117 114 107 7 1 6 1 4 0 0 0 0 0 0 0 1 1 0 8 8 6 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcacctttagtagtttctgg atgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgt acaagggacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttagtagtttctgg atgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgt acaagggacatcggtgggcgctactcctac IGA2 21 213 13 8 9 13 4 8 3 2 9 7 JY8QFUQ01A7TRV 10600 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD2-21*02 F 3 8 8 17 8.8.17 [1.17.38.11] CAKDLVVTSIGPQGIHGHW in-frame + ctctgggttcacctttagcagctatgccatgacttgggtccgccaggctccagggaaggggctggagtgggtctcaagtatcagttttagtggtgagagaacatattatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacagtacatttgcaaatggacagcctgagagccgaggacacggccgtatattactgtgcgaaagatctggtggtgactagtattggcccccagggaatccatggccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 71 100 [299] a81>g|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t168>c|g172>t,G58>F(- - -)|g173>t,G58>F(- - -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)|c198>t|c201>t|t246>c|g258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t262>c,Y88>H(+ - -)|c265>t|a274>g,N92>D(+ + -)| a81>g| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| t168>c|g172>t,G58>F(- - -)|g173>t,G58>F(- - -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)| c198>t|c201>t|t246>c|g258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t262>c,Y88>H(+ - -)|c265>t|a274>g,N92>D(+ + -)| 320 225 205 20 7 13 2 4 4 3 0 1 0 0 1 2 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 51 51 47 4 0 4 0 1 1 0 0 0 0 0 1 1 0 0 30 24 18 6 1 5 0 1 0 1 0 1 0 0 0 0 2 1 117 114 105 9 5 4 1 2 3 2 0 0 0 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcacctttagcagctatgcc atgacttgggtccgccaggctccagggaaggggctggagtgggtctcaagt atcagttttagtggtgagagaaca tattatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacagtacatttgcaaatggacagcctgagagccgaggacacggccgtatattactgt gcgaaagatctggtggtgactagtattggcccccagggaatccatggccac AKDLVVTSIGPQGIHGH IGHV3-23 IGHD2-21 IGHJ4 gggttcacctttagcagctatgcc atgacttgggtccgccaggctccagggaaggggctggagtgggtctcaagt atcagttttagtggtgagagaaca tattatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacagtacatttgcaaatggacagcctgagagccgaggacacggccgtatattactgt gcgaaagatctggtggtgactagtattggcccccagggaatccatggccac IGA1 20 213 13 7 8 14 5 6 5 2 8 5 JY8QFUQ01A7TW1 2840 productive Homsap IGHV3-48*04 F 990 95.39 207/217 nt NA Homsap IGHJ4*02 F (see comment) 222 95.83 46/48 nt Homsap IGHD6-19*01 F 1 8 8 16 8.8.16 [1.17.38.11] CARDAGGSGWYLYYFDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcaccttcagtagttataccatgcactgggtccgccaggctccagggaaggggctggaatgggtctcatccattagtagtagtagtactaccatatactacgcagactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacggcctgagagccgaggacacggctgtttattcctgtgcgagagatgcggggggcagtggctggtacctttactactttgactcctggggccagggaaccctggtcaccgtctcctcaacatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtcagggcttcttccc NA IGA1 100 100 [296] c108>t|g113>c,S38>T(+ - +)|a118>c,N40>H(- - -)|g153>a|t159>c|a164>c,Y55>S(+ - -)|g188>c,S63>T(+ - +)|a277>g,S93>G(+ + -)|g303>t|a308>c,Y103>S(+ - -)| c108>t|g113>c,S38>T(+ - +)| a118>c,N40>H(- - -)|g153>a|t159>c|a164>c,Y55>S(+ - -)| g188>c,S63>T(+ - +)| a277>g,S93>G(+ + -)|g303>t|a308>c,Y103>S(+ - -)| 320 225 215 10 4 6 1 1 1 1 3 0 0 0 2 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 1 0 0 0 0 0 1 0 0 0 51 51 47 4 2 2 0 1 0 1 2 0 0 0 0 0 0 0 30 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 117 114 111 3 1 2 1 0 0 0 1 0 0 0 0 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagttatacc atgcactgggtccgccaggctccagggaaggggctggaatgggtctcatcc attagtagtagtagtactaccata tactacgcagactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacggcctgagagccgaggacacggctgtttattcctgt gcgagagatgcggggggcagtggctggtacctttactactttgactcc ARDAGGSGWYLYYFDS IGHV3-48 IGHD6-19 IGHJ4 ggattcaccttcagtagttatacc atgcactgggtccgccaggctccagggaaggggctggaatgggtctcatcc attagtagtagtagtactaccata tactacgcagactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacggcctgagagccgaggacacggctgtttattcctgt gcgagagatgcggggggcagtggctggtacctttactactttgactcc IGA1 10 213 4 6 2 5 2 5 3 1 4 2 JY8QFUQ01A7XAB 14736 productive Homsap IGHV4-4*07 F 890 90.7 195/215 nt NA Homsap IGHJ3*02 F 214 92 46/50 nt Homsap IGHD4-17*01 F 3 8 7 14 8.7.14 [1.17.38.11] CARDLPTVTTGALDIW in-frame + tctctggtggctccttcagtaattaccactggagctggatccggaagcccgccgggaaggaactggagtggattgggcgtatccatcacagtgggctcaccaactccaacccctccctcaagaatcgagtcgtcgtgtcagtggatacgtccaagaatcagttgtccctggagctgagctctgtgaccgccgcggacacggccgtgtattactgtgcgagagatcttcctacggtgactactggggctcttgatatctggggccaagggacaatggtcaccgtctctccagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggcagcacctccgagagcacagcggccctgggctgccctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 86 88 [289, 290] a88>t,I30>F(+ - -)|g107>a,S36>N(- - -)|t112>c,Y38>H(+ - -)|c130>a,Q44>K(+ - -)|g146>a,G49>E(- - -)|t169>c,Y57>H(+ - -)|a172>c,T58>H(+ - -)|c173>a,T58>H(+ - -)|a190>c,S64>L(- - -)|g191>t,S64>L(- - -)|a200>c,Y67>S(+ - -)|g221>a,S74>N(- - -)|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|a232>g,M78>V(+ - -)|a240>g|c243>t|c255>t|c261>g,F87>L(+ - -)|a268>g,K90>E(+ - -)| a88>t,I30>F(+ - -)|g107>a,S36>N(- - -)|t112>c,Y38>H(+ - -)| c130>a,Q44>K(+ - -)|g146>a,G49>E(- - -)| t169>c,Y57>H(+ - -)|a172>c,T58>H(+ - -)|c173>a,T58>H(+ - -)|a190>c,S64>L(- - -)|g191>t,S64>L(- - -)| a200>c,Y67>S(+ - -)|g221>a,S74>N(- - -)|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|a232>g,M78>V(+ - -)|a240>g|c243>t|c255>t|c261>g,F87>L(+ - -)|a268>g,K90>E(+ - -)| 320 223 203 20 3 17 4 3 3 2 3 2 1 0 0 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 1 0 0 1 0 0 0 0 0 51 51 49 2 0 2 0 1 0 0 0 1 0 0 0 0 0 0 30 21 16 5 0 5 0 0 0 1 2 1 0 0 0 0 1 0 117 114 104 10 3 7 4 1 3 0 1 0 0 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccttcagtaattaccac tggagctggatccggaagcccgccgggaaggaactggagtggattgggcgt atccatcacagtgggctcacc aactccaacccctccctcaagaatcgagtcgtcgtgtcagtggatacgtccaagaatcagttgtccctggagctgagctctgtgaccgccgcggacacggccgtgtattactgt gcgagagatcttcctacggtgactactggggctcttgatatc ARDLPTVTTGALDI IGHV4-4 IGHD4-17 IGHJ3 ggtggctccttcagtaattaccac tggagctggatccggaagcccgccgggaaggaactggagtggattgggcgt atccatcacagtgggctcacc aactccaacccctccctcaagaatcgagtcgtcgtgtcagtggatacgtccaagaatcagttgtccctggagctgagctctgtgaccgccgcggacacggccgtgtattactgt gcgagagatcttcctacggtgactactggggctcttgatatc IGG2 20 210 12 8 6 10 6 10 3 0 9 8 JY8QFUQ01A7XDX 11453 productive Homsap IGHV3-7*01 F 999 95.85 208/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSGYYSTGYW in-frame - ctctggattcacctttagtacctattggatgagctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagcatgatgcaagtgagaaatactatgtggactctgtaaaaggccgattcaccatctccagagacaacgccaagaactcattgtatttacaaatgaacaacctgagagccgaggacacggctgtgtattactgtgctattagtagtggttattactcgacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [281] g107>c,S36>T(+ - +)|a174>t,Q58>H(- + -)|g185>c,G62>A(- + -)|g213>a|g216>a|c259>t|c265>t|g267>a|g278>a,S93>N(- - -)| g107>c,S36>T(+ - +)| a174>t,Q58>H(- + -)|g185>c,G62>A(- + -)| g213>a|g216>a|c259>t|c265>t|g267>a|g278>a,S93>N(- - -)| g315>t|g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 314 (320) 219 (225) 210 (211) 9 (14) 5 (8) 4 (6) 0 (1) 4 (5) 2 0 0 0 1 (2) 0 2 0 0 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 1 0 1 0 0 0 117 114 108 6 5 1 0 4 2 0 0 0 0 0 0 0 0 0 2 (8) 2 2 (3) 0 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (2) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtacctattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcatgatgcaagtgagaaa tactatgtggactctgtaaaaggccgattcaccatctccagagacaacgccaagaactcattgtatttacaaatgaacaacctgagagccgaggacacggctgtgtattactgt gctattagtagtggttattactcgacgggctac AISSGYYSTGY IGHV3-7 IGHD3-22 IGHJ4 ggattcacctttagtacctattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcatgatgcaagtgagaaa tactatgtggactctgtaaaaggccgattcaccatctccagagacaacgccaagaactcattgtatttacaaatgaacaacctgagagccgaggacacggctgtgtattactgt gctattagtagtggttattactcgacgggctac IGA2 9 213 6 3 6 8 0 1 5 0 1 3 JY8QFUQ01A7Z2B 10209 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 950 93.12 203/218 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD3-16*01 F 2 8 8 16 8.8.16 [1.17.38.11] CARRLNNYDSGEYAMDVW in-frame + gttctggatacatgtttatgaattactggatcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatcatctatcctggtacctctgagaccagatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgcctacgtgcagtggagcagcctgaaggccccggacaccgccatatattactgtgcgaggcgactcaataattacgactcgggagagtacgctatggacgtctggggccaagggaccacggtcaccgtctcctcaacctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [298] g86>t,S29>M(- - -)|c87>g,S29>M(- - -)|c104>t,T35>M(- - -)|c105>g,T35>M(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g184>a,D62>T(- + -)|a185>c,D62>T(- + -)|t192>g,D64>E(+ - +)|c213>t|c234>t|t251>c,I84>T(- - -)|c265>g,L89>V(+ - +)|t289>c,S97>P(+ - -)|g303>a,M101>I(+ + -)| g86>t,S29>M(- - -)|c87>g,S29>M(- - -)|c104>t,T35>M(- - -)|c105>g,T35>M(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g184>a,D62>T(- + -)|a185>c,D62>T(- + -)|t192>g,D64>E(+ - +)| c213>t|c234>t|t251>c,I84>T(- - -)|c265>g,L89>V(+ - +)|t289>c,S97>P(+ - -)|g303>a,M101>I(+ + -)| a318>g|a320>g| 317 (320) 223 (226) 208 (209) 15 (17) 2 (4) 13 0 (2) 3 4 2 1 0 0 0 0 3 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 1 2 0 0 0 0 0 0 2 1 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 1 0 0 1 0 0 0 0 0 0 1 117 114 108 6 2 4 0 1 2 2 0 0 0 0 0 1 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| gttct ggatacatgtttatgaattactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtacctctgagacc agatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgcctacgtgcagtggagcagcctgaaggccccggacaccgccatatattactgt gcgaggcgactcaataattacgactcgggagagtacgctatggacgtc ARRLNNYDSGEYAMDV IGHV5-51 IGHD3-16 IGHJ6 ggatacatgtttatgaattactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtacctctgagacc agatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgcctacgtgcagtggagcagcctgaaggccccggacaccgccatatattactgt gcgaggcgactcaataattacgactcgggagagtacgctatggacgtc IGG1 15 213 9 6 7 11 2 4 2 0 4 9 JY8QFUQ01A7Z8B 13977 productive (see comment) Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 956 92.76 205/221 nt 91.86 203/221 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD5-24*01 ORF 1 7 10 10 7.10.10 [1.17.38.11] CTHLREEMSYEW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 55 to 55 in the user submitted sequence), (cause frameshift); in CDR1-IMGT, from codon 29 of V-REGION: 1 nucleotides (from position 11 in the user submitted sequence), (cause frameshift) tctggagtcaattcagaaacgcctggatgaattgggtccgccaggctccagggaAaggggctggagtgggttggccgtattaagagcaaagctgatggtgggacaacagactacgccacacccgtgagaggcagattcaccatctcaagagatgattcaaaaaacacgttttatctgcaaatgaatagcctaaaaaccgaagacacagccgtctattactgtacccacttgcgagaagagatgtcttacgaatggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [283] t82>g,F28>V(+ - -)|t87>a|t105>a,S35>R(- - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a171>g|a178>g,T60>A(- - -)|t204>c|g205>a,A69>T(- - -)|a215>g,K72>R(+ + +)|c259>t,L87>F(+ - -)|g261>t,L87>F(+ - -)|c276>t|g282>a|g291>a|g303>c| t82>g,F28>V(+ - -)|t87>a|t105>a,S35>R(- - -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)| a171>g|a178>g,T60>A(- - -)| t204>c|g205>a,A69>T(- - -)|a215>g,K72>R(+ + +)|c259>t,L87>F(+ - -)|g261>t,L87>F(+ - -)|c276>t|g282>a|g291>a|g303>c| a316>c,T106>H(+ - -)|c317>a,T106>H(+ - -)|a318>c,T106>H(+ - -)|g319>t|a320>t| 315 (320) 224 (229) 208 16 (21) 7 (9) 9 (12) 3 4 3 1 0 (2) 0 (1) 0 (1) 2 1 0 1 (2) 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 20 3 1 2 0 0 0 0 0 0 0 2 0 0 0 1 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 30 28 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 117 114 105 9 5 4 1 3 2 1 0 0 0 0 1 0 1 0 3 (8) 3 3 0 (5) 0 (2) 0 (3) 0 0 0 0 0 (2) 0 (1) 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| tct ggagtcaattcagaaacgcctgg atgaattgggtccgccaggctccagggaaggggctggagtgggttggccgt attaagagcaaagctgatggtgggacaaca gactacgccacacccgtgagaggcagattcaccatctcaagagatgattcaaaaaacacgttttatctgcaaatgaatagcctaaaaaccgaagacacagccgtctattactgt acccacttgcgagaagagatgtcttacgaa THLREEMSYE IGHV3-15 IGHD5-24 IGHJ4 ggagtcaattcagaaacgcctgg atgaattgggtccgccaggctccagggaaggggctggagtgggttggccgt attaagagcaaagctgatggtgggacaaca gactacgccacacccgtgagaggcagattcaccatctcaagagatgattcaaaaaacacgttttatctgcaaatgaatagcctaaaaaccgaagacacagccgtctattactgt acccacttgcgagaagagatgtcttacgaa IGA1 16 218 11 5 7 9 4 7 5 2 6 3 JY8QFUQ01A82FB 20389 productive Homsap IGHV4-31*02 F, or Homsap IGHV4-31*03 F or Homsap IGHV4-31*04 F 947 92.31 204/221 nt NA Homsap IGHJ5*02 F (see comment) 174 82.35 42/51 nt Homsap IGHD6-19*01 F 1 10 7 10 10.7.10 [1.17.38.11] CAAYDSGWYKHW in-frame - Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) tctctggtggctccgtcaacagtggtgattactactggacctggatccgccagcaccctgggaagggcctggagtggattggatacatctattacagtgggagcacttactacaatccgtccctggagagtcgagttaccatatcaatagacatgtctaagaaccagttctccctgaaagtgagctctgtgactgccgcggatacggccgtgtatcattgtgcggcgtatgacagtggctggtacaaacactggggccagggaaccctggtcaccgtgtcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [282] a88>g,I30>V(+ - +)|g92>a,S31>N(- - -)|g107>a,G36>D(- - -)|g119>c,S40>T(+ - +)|a138>t|g162>a|c195>t|c204>t|c213>g|a214>g,K72>E(+ - -)|g238>a,V80>I(+ - +)|c245>t,T82>M(- - -)|g270>a|c271>g,L91>V(+ - +)|c294>t|t307>c,Y103>H(+ - -)|c309>t,Y103>H(+ - -)| a88>g,I30>V(+ - +)|g92>a,S31>N(- - -)|g107>a,G36>D(- - -)| g119>c,S40>T(+ - +)|a138>t|g162>a| c195>t| c204>t|c213>g|a214>g,K72>E(+ - -)|g238>a,V80>I(+ - +)|c245>t,T82>M(- - -)|g270>a|c271>g,L91>V(+ - +)|c294>t|t307>c,Y103>H(+ - -)|c309>t,Y103>H(+ - -)| a316>g,R106>A(- - -)|g317>c,R106>A(- - -)|a318>g,R106>A(- - -)|g319>t| 315 (320) 224 (229) 207 (208) 17 (21) 7 (8) 10 (13) 2 (4) 5 5 1 0 0 1 0 1 (2) 2 0 (1) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 1 2 0 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 0 0 0 0 1 0 1 0 0 0 30 21 20 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 117 114 104 10 4 6 1 2 4 1 0 0 0 0 0 2 0 0 3 (8) 3 3 (4) 0 (4) 0 (1) 0 (3) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 aa,40-41(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,228-229(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,69-70(FR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,227-228(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|agca,131-134(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agtt,225-228(FR3)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,69-72(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|tacc,228-231(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tgcc,285-288(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcaacagtggtgattactac tggacctggatccgccagcaccctgggaagggcctggagtggattggatac atctattacagtgggagcact tactacaatccgtccctggagagtcgagttaccatatcaatagacatgtctaagaaccagttctccctgaaagtgagctctgtgactgccgcggatacggccgtgtatcattgt gcggcgtatgacagtggctggtacaaacac AAYDSGWYKH IGHV4-31 IGHD6-19 IGHJ5 ggtggctccgtcaacagtggtgattactac tggacctggatccgccagcaccctgggaagggcctggagtggattggatac atctattacagtgggagcact tactacaatccgtccctggagagtcgagttaccatatcaatagacatgtctaagaaccagttctccctgaaagtgagctctgtgactgccgcggatacggccgtgtatcattgt gcggcgtatgacagtggctggtacaaacac IGA1 17 216 13 4 10 13 3 4 6 1 7 3 JY8QFUQ01A82HJ 21728 productive Homsap IGHV3-72*01 F 953 91.96 206/224 nt NA Homsap IGHJ6*02 F 193 79.03 49/62 nt Homsap IGHD6-19*01 F 1 8 10 14 8.10.14 [1.17.38.11] CARLHYSGGWYGLDVW in-frame - cctctggattcaccttcagtgaccactacatagactgggtccgccaggctccaggaaaggggctggagtgggttggccgtactcgaaataaagctaacggttacagtacagagtatgccgcgtctgtgaaaggcagattcaccgtctcaagagataactcagagaacttagtgcatctgcaaatgaacagcctgaaaagcgaggacacggccctgtattactgtgctagactgcactacagtggtggctggtacggtctggacgtctggggccaagggaccacagtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] g117>a,M39>I(+ + -)|g141>a|a169>c|c174>t|a184>g,S62>G(+ + -)|c191>g,T64>S(+ - +)|c192>t,T64>S(+ - +)|a198>g|c201>t|a232>g,I78>V(+ - +)|g244>a,D82>N(+ + -)|t246>c,D82>N(+ + -)|a250>g,K84>E(+ - -)|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)|c287>g,T96>S(+ - +)|g301>c,V101>L(+ - +)| g117>a,M39>I(+ + -)|g141>a| a169>c|c174>t|a184>g,S62>G(+ + -)|c191>g,T64>S(+ - +)|c192>t,T64>S(+ - +)| a198>g|c201>t|a232>g,I78>V(+ - +)|g244>a,D82>N(+ + -)|t246>c,D82>N(+ + -)|a250>g,K84>E(+ - -)|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)|c287>g,T96>S(+ - +)|g301>c,V101>L(+ - +)| g319>c|a320>t| 318 (320) 230 (232) 212 18 (20) 5 (7) 13 4 3 4 2 1 0 0 (1) 0 1 (2) 3 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 30 30 25 5 2 3 1 0 2 0 1 0 0 0 0 1 0 0 117 114 103 11 2 9 3 1 2 2 0 0 0 0 1 2 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,165-168(FR2-CDR2)|agct,177-180(CDR2)|aact,253-256(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tgct,312-315(FR3)| cctct ggattcaccttcagtgaccactac atagactgggtccgccaggctccaggaaaggggctggagtgggttggccgt actcgaaataaagctaacggttacagtaca gagtatgccgcgtctgtgaaaggcagattcaccgtctcaagagataactcagagaacttagtgcatctgcaaatgaacagcctgaaaagcgaggacacggccctgtattactgt gctagactgcactacagtggtggctggtacggtctggacgtc ARLHYSGGWYGLDV IGHV3-72 IGHD6-19 IGHJ6 ggattcaccttcagtgaccactac atagactgggtccgccaggctccaggaaaggggctggagtgggttggccgt actcgaaataaagctaacggttacagtaca gagtatgccgcgtctgtgaaaggcagattcaccgtctcaagagataactcagagaacttagtgcatctgcaaatgaacagcctgaaaagcgaggacacggccctgtattactgt gctagactgcactacagtggtggctggtacggtctggacgtc IGA1 18 219 13 5 7 11 6 7 3 2 10 3 JY8QFUQ01A82RF 5274 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 858 89.25 191/214 nt NA Homsap IGHJ1*01 F 224 92.31 48/52 nt Homsap IGHD3-10*01 F 2 8 7 14 8.7.14 [1.17.38.11] CASGGYSGSGEYFLRW in-frame + ctctgacttaagcgtcagtgacaattacatgacctgggtccgccaggctccagggaaggggctggagtgggtctcaattatgtatagcggaggtcgcacatactacgcagagtccgtgaagggccgattcgccgtctccagagacaattcccagaatacactgtatcttcaaatgaacagcctgaggaccgaagacacggccgtgtactattgtgcgagtggtggttattcgggctcgggtgaatacttcctacgctggggccagggcaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [287] g80>a,G27>D(- - -)|g81>c,G27>D(- - -)|c84>a,F28>L(+ - -)|c86>g,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c111>t|g119>c,S40>T(+ - +)|g163>a,V55>I(+ - +)|t168>g,I56>M(+ + -)|t177>a|a190>c,S64>R(- - -)|c207>g,D69>E(+ - +)|a229>g,T77>A(- - -)|a232>g,I78>V(+ - +)|a250>c,K84>Q(+ - -)|c255>t|g258>a|a285>g|g286>a,A96>T(- - -)|g291>a|t306>c|c309>t| g80>a,G27>D(- - -)|g81>c,G27>D(- - -)|c84>a,F28>L(+ - -)|c86>g,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c111>t| g119>c,S40>T(+ - +)|g163>a,V55>I(+ - +)| t168>g,I56>M(+ + -)|t177>a|a190>c,S64>R(- - -)| c207>g,D69>E(+ - +)|a229>g,T77>A(- - -)|a232>g,I78>V(+ - +)|a250>c,K84>Q(+ - -)|c255>t|g258>a|a285>g|g286>a,A96>T(- - -)|g291>a|t306>c|c309>t| a318>t,R106>S(- - -)|a320>g| 317 (320) 219 (222) 196 (197) 23 (25) 8 (9) 15 (16) 4 (5) 6 3 1 2 1 0 (1) 1 2 2 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 1 6 1 2 1 0 0 1 0 0 1 1 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 30 21 18 3 1 2 0 0 0 0 1 0 0 1 0 0 0 1 117 114 103 11 6 5 3 3 2 1 1 0 0 0 0 1 0 0 5 (8) 5 5 (6) 0 (2) 0 (1) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gacttaagcgtcagtgacaattac atgacctgggtccgccaggctccagggaaggggctggagtgggtctcaatt atgtatagcggaggtcgcaca tactacgcagagtccgtgaagggccgattcgccgtctccagagacaattcccagaatacactgtatcttcaaatgaacagcctgaggaccgaagacacggccgtgtactattgt gcgagtggtggttattcgggctcgggtgaatacttcctacgc ASGGYSGSGEYFLR IGHV3-53 IGHD3-10 IGHJ1 gacttaagcgtcagtgacaattac atgacctgggtccgccaggctccagggaaggggctggagtgggtctcaatt atgtatagcggaggtcgcaca tactacgcagagtccgtgaagggccgattcgccgtctccagagacaattcccagaatacactgtatcttcaaatgaacagcctgaggaccgaagacacggccgtgtactattgt gcgagtggtggttattcgggctcgggtgaatacttcctacgc IGA1 23 210 14 9 9 14 5 9 6 2 7 8 JY8QFUQ01A82RW 8813 productive Homsap IGHV3-30*10 F 927 92.17 200/217 nt NA Homsap IGHJ6*02 F (see comment) 193 79.03 49/62 nt Homsap IGHD3-9*01 F 1 8 8 15 8.8.15 [1.17.38.11] CARDRDFDFPAYGLDVW in-frame + Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ctctggattcaacttcagtaactatgctgtgcactgggtccgccgggctccaggcaaggggctagagtggatgggagttatatcatttgatggagataataaatactacacagactccgtgaagggccgattcaccatgtctagagacaattccaagaacacactatatctccaaatgaacagcctgtcagctgaggacacggctgtctattactgtgcgagagatcgagatttcgactttcctgcttacggtttggacgtctggggccaagggaccacggtcaccgtctcctccgcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [294] c86>a,T29>N(- + -)|g107>a,S36>N(- - -)|a115>g,M39>V(+ - -)|a131>g,Q44>R(+ - -)|g157>a,V53>M(+ - -)|c161>g,A54>G(- + -)|a173>t,Y58>F(- + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|c234>g,I78>M(+ + -)|c237>t|g258>a|g261>a|g267>c|a283>t,R95>S(- - -)|g284>c,R95>S(- - -)|g303>c| c86>a,T29>N(- + -)|g107>a,S36>N(- - -)| a115>g,M39>V(+ - -)|a131>g,Q44>R(+ - -)|g157>a,V53>M(+ - -)|c161>g,A54>G(- + -)| a173>t,Y58>F(- + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)| c234>g,I78>M(+ + -)|c237>t|g258>a|g261>a|g267>c|a283>t,R95>S(- - -)|g284>c,R95>S(- - -)|g303>c| 320 225 208 17 5 12 3 5 1 0 0 1 2 0 3 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 1 0 0 0 0 0 0 51 51 47 4 0 4 2 1 0 0 0 0 0 0 0 1 0 0 30 24 21 3 0 3 1 1 0 0 0 0 1 0 0 0 0 0 117 114 106 8 5 3 0 2 1 0 0 0 1 0 3 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaacttcagtaactatgct gtgcactgggtccgccgggctccaggcaaggggctagagtggatgggagtt atatcatttgatggagataataaa tactacacagactccgtgaagggccgattcaccatgtctagagacaattccaagaacacactatatctccaaatgaacagcctgtcagctgaggacacggctgtctattactgt gcgagagatcgagatttcgactttcctgcttacggtttggacgtc ARDRDFDFPAYGLDV IGHV3-30 IGHD3-9 IGHJ6 ggattcaacttcagtaactatgct gtgcactgggtccgccgggctccaggcaaggggctagagtggatgggagtt atatcatttgatggagataataaa tactacacagactccgtgaagggccgattcaccatgtctagagacaattccaagaacacactatatctccaaatgaacagcctgtcagctgaggacacggctgtctattactgt gcgagagatcgagatttcgactttcctgcttacggtttggacgtc IGG1 17 213 9 8 6 12 3 5 5 0 7 5 JY8QFUQ01A8412 17913 productive Homsap IGHV3-7*01 F 909 91.24 198/217 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD4-17*01 F 3 8 8 14 8.8.14 [1.17.38.11] CATSPFTVTTGKDAYW in-frame + ctctagattcacctttaatggttactggatgagttgggtccgccaggctccaggaaaggggctggagtggctggccaacataaagccggatggaaatgagaaatgctatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagagttcgctgtttctgcaaatgaacagcctgagagccgaggacacggctgtatatttctgtgcgacctccccctttacggtgactactgggaaggacgcctactggggccagggaaccctggtcctcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [290] g79>a,G27>R(- - -)|g104>a,S35>N(- - -)|a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)|t111>c|c120>t|g141>a|g157>c,V53>L(+ - +)|a173>c,Q58>P(- - -)|a174>g,Q58>P(- - -)|g188>a,S63>N(- - -)|a197>g,Y66>C(- - -)|t203>c,V68>A(+ - +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a258>g|a263>t,Y88>F(- + -)|g303>a|a308>t,Y103>F(- + -)| g79>a,G27>R(- - -)|g104>a,S35>N(- - -)|a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)|t111>c| c120>t|g141>a|g157>c,V53>L(+ - +)| a173>c,Q58>P(- - -)|a174>g,Q58>P(- - -)|g188>a,S63>N(- - -)| a197>g,Y66>C(- - -)|t203>c,V68>A(+ - +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a258>g|a263>t,Y88>F(- + -)|g303>a|a308>t,Y103>F(- + -)| g317>c,R106>T(- - -)|a318>c,R106>T(- - -)|g319>t|a320>c| 316 (320) 221 (225) 202 19 (23) 5 (7) 14 (16) 5 5 3 2 1 (3) 0 2 0 1 (2) 0 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 1 2 1 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 0 1 0 0 0 30 24 21 3 0 3 1 1 0 0 1 0 0 0 0 0 0 0 117 114 106 8 2 6 3 1 1 1 0 0 2 0 0 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 0 0 0 0 (2) 0 0 0 0 (1) 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct agattcacctttaatggttactgg atgagttgggtccgccaggctccaggaaaggggctggagtggctggccaac ataaagccggatggaaatgagaaa tgctatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagagttcgctgtttctgcaaatgaacagcctgagagccgaggacacggctgtatatttctgt gcgacctccccctttacggtgactactgggaaggacgcctac ATSPFTVTTGKDAY IGHV3-7 IGHD4-17 IGHJ4 agattcacctttaatggttactgg atgagttgggtccgccaggctccaggaaaggggctggagtggctggccaac ataaagccggatggaaatgagaaa tgctatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagagttcgctgtttctgcaaatgaacagcctgagagccgaggacacggctgtatatttctgt gcgacctccccctttacggtgactactgggaaggacgcctac IGA2 19 213 15 4 8 9 7 10 4 1 7 7 JY8QFUQ01A8464 18289 productive Homsap IGHV4-61*02 F 956 92.76 205/221 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-19*01 F 1 10 7 14 10.7.14 [1.17.38.11] CAREPQSNAWYYFDHW in-frame + tctctggtggctccgtcaacagtggtaatttctactggagctggatccggcagcccgccgggaagggactggagtggatagggcgtatctatgccagtgggagcaccaactacaacccctccctcaagagtcgaatcaccatatcagcagacacatccaagaatcagttctccctgaggctgagttctgtgaccgccgcagacacaggcgtttattattgtgcgagagaaccacaaagcaatgcctggtactactttgaccactggggccggggagccctggtcaccgtctccacagcctccagccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 93 88 [296] a88>g,I30>V(+ - +)|g92>a,S31>N(- - -)|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)|t159>a|a172>g,T58>A(- - -)|g226>a,V76>I(+ - +)|t239>c,V80>A(+ - +)|g246>a|c255>t|a269>g,K90>R(+ + +)|c276>t|g297>a|c299>g,A100>G(- + -)|g303>t|c309>t| a88>g,I30>V(+ - +)|g92>a,S31>N(- - -)|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)| t159>a| a172>g,T58>A(- - -)| g226>a,V76>I(+ - +)|t239>c,V80>A(+ - +)|g246>a|c255>t|a269>g,K90>R(+ + +)|c276>t|g297>a|c299>g,A100>G(- + -)|g303>t|c309>t| 320 229 213 16 7 9 3 5 3 1 0 0 1 1 0 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 0 4 1 2 0 0 0 0 1 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 21 20 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 117 114 104 10 6 4 1 3 3 1 0 0 0 0 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcaacagtggtaatttctac tggagctggatccggcagcccgccgggaagggactggagtggatagggcgt atctatgccagtgggagcacc aactacaacccctccctcaagagtcgaatcaccatatcagcagacacatccaagaatcagttctccctgaggctgagttctgtgaccgccgcagacacaggcgtttattattgt gcgagagaaccacaaagcaatgcctggtactactttgaccac AREPQSNAWYYFDH IGHV4-61 IGHD6-19 IGHJ4 ggtggctccgtcaacagtggtaatttctac tggagctggatccggcagcccgccgggaagggactggagtggatagggcgt atctatgccagtgggagcacc aactacaacccctccctcaagagtcgaatcaccatatcagcagacacatccaagaatcagttctccctgaggctgagttctgtgaccgccgcagacacaggcgtttattattgt gcgagagaaccacaaagcaatgcctggtactactttgaccac IGG2 16 216 12 4 8 10 4 6 7 0 4 5 JY8QFUQ01A86GB 1723 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 851 88.07 192/218 nt NA Homsap IGHJ6*02 F 247 88.71 55/62 nt Homsap IGHD1-7*01 F 3 8 8 19 8.8.19 [1.17.38.11] CAKVFQVPGEHHFYYFGMNVW in-frame - cctctggattcatgtttaggaactatgccatgagttgggtccgccaggctccagggagggggctggagtgggtctcaactattagcaataatggaagccacacatactacgcagactccgtcaagggccggttcaccatctccagagacaattccaagaacacggtgtatatgcagatgaacaggctgagagtcgaggagacggccctgtactactgtgcgaaagtcttccaggtgcctggcgagcaccacttctactacttcggaatgaatgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [306] c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|c105>g,S35>R(- - -)|g107>a,S36>N(- - -)|c120>t|a143>g,K48>R(+ + +)|g163>a,A55>T(- - -)|t171>c|g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|g176>a,S59>N(- - -)|t186>a|g187>a,G63>S(+ + -)|t189>c,G63>S(+ + -)|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|g213>c|c259>g,L87>V(+ - +)|c265>a,L89>M(+ + -)|a270>g|c279>g,S93>R(- - -)|c287>t,A96>V(+ - +)|c294>g,D98>E(+ - +)|g301>c,V101>L(+ - +)|a303>g,V101>L(+ - +)|t306>c| c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|c105>g,S35>R(- - -)|g107>a,S36>N(- - -)| c120>t|a143>g,K48>R(+ + +)|g163>a,A55>T(- - -)| t171>c|g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|g176>a,S59>N(- - -)|t186>a|g187>a,G63>S(+ + -)|t189>c,G63>S(+ + -)|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)| g213>c|c259>g,L87>V(+ - +)|c265>a,L89>M(+ + -)|a270>g|c279>g,S93>R(- - -)|c287>t,A96>V(+ - +)|c294>g,D98>E(+ - +)|g301>c,V101>L(+ - +)|a303>g,V101>L(+ - +)|t306>c| a320>t| 319 (320) 225 (226) 199 26 (27) 6 (7) 20 3 7 3 3 1 1 0 (1) 1 2 5 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 1 0 0 0 0 0 0 2 0 0 51 51 48 3 1 2 1 1 1 0 0 0 0 0 0 0 0 0 30 24 15 9 2 7 0 5 0 2 1 0 0 1 0 0 0 0 117 114 104 10 3 7 2 0 1 1 0 1 0 0 2 3 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcatgtttaggaactatgcc atgagttgggtccgccaggctccagggagggggctggagtgggtctcaact attagcaataatggaagccacaca tactacgcagactccgtcaagggccggttcaccatctccagagacaattccaagaacacggtgtatatgcagatgaacaggctgagagtcgaggagacggccctgtactactgt gcgaaagtcttccaggtgcctggcgagcaccacttctactacttcggaatgaatgtc AKVFQVPGEHHFYYFGMNV IGHV3-23 IGHD1-7 IGHJ6 ggattcatgtttaggaactatgcc atgagttgggtccgccaggctccagggagggggctggagtgggtctcaact attagcaataatggaagccacaca tactacgcagactccgtcaagggccggttcaccatctccagagacaattccaagaacacggtgtatatgcagatgaacaggctgagagtcgaggagacggccctgtactactgt gcgaaagtcttccaggtgcctggcgagcaccacttctactacttcggaatgaatgtc IGA1 26 213 16 10 10 18 6 8 4 2 9 11 JY8QFUQ01A86IT 14392 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 777 85.05 182/214 nt NA Homsap IGHJ6*02 F (see comment) 175 75.81 47/62 nt Homsap IGHD3-10*01 F 2 8 7 14 8.7.14 [1.17.38.11] CARERGSGPPTGMDVW in-frame + Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*03 (shorter alignment but highest percentage of identity) ttatgggttgaccgtcggtgccgaccacatgtactgggtccgccaggctccagggaaggggctgaagtgggtctcagttctttatggcggtggcaccttggactacgcagactccgtgaagggccgattcaccatctccagagacaactcgaggaacactgtgtatcttcagatggagagactgagccccgaggacacggccgtctactactgtgcgagagaaagaggttcgggcccccccacaggcatggacgtctggggcccagggaccatggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 79 94 [288] c75>t|c77>a,S26>Y(+ - -)|c84>g,F28>L(+ - -)|a103>g,S35>G(+ + -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a109>g,N37>D(+ + -)|t112>c,Y38>H(+ - -)|a118>t,S40>Y(+ - -)|g119>a,S40>Y(+ - -)|g151>a,E51>K(+ - -)|a166>c,I56>L(+ + +)|a172>g,S58>G(+ + -)|t189>c|g191>c,S64>T(+ - +)|a193>t,T65>L(- - -)|c194>t,T65>L(- - -)|a195>g,T65>L(- - -)|t196>g,Y66>D(- - -)|t246>c|c249>g|a251>g,K84>R(+ + +)|g258>t|c259>g,L87>V(+ - +)|a270>g|a274>g,N92>E(+ - -)|c276>g,N92>E(+ - -)|c279>a,S93>R(- - -)|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|g303>c|t306>c| c75>t|c77>a,S26>Y(+ - -)| c84>g,F28>L(+ - -)|a103>g,S35>G(+ + -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a109>g,N37>D(+ + -)|t112>c,Y38>H(+ - -)| a118>t,S40>Y(+ - -)|g119>a,S40>Y(+ - -)|g151>a,E51>K(+ - -)| a166>c,I56>L(+ + +)|a172>g,S58>G(+ + -)|t189>c|g191>c,S64>T(+ - +)|a193>t,T65>L(- - -)|c194>t,T65>L(- - -)|a195>g,T65>L(- - -)| t196>g,Y66>D(- - -)|t246>c|c249>g|a251>g,K84>R(+ + +)|g258>t|c259>g,L87>V(+ - +)|a270>g|a274>g,N92>E(+ - -)|c276>g,N92>E(+ - -)|c279>a,S93>R(- - -)|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|g303>c|t306>c| 320 222 190 32 8 24 8 2 2 4 2 2 2 0 4 4 1 1 78 4 2 2 1 1 0 0 1 0 0 1 0 0 0 0 0 0 36 24 18 6 0 6 3 0 0 1 0 0 0 0 1 1 0 0 51 51 48 3 0 3 0 2 0 0 0 0 1 0 0 0 0 0 30 21 14 7 1 6 2 0 1 1 1 0 1 0 1 0 0 0 117 114 100 14 6 8 3 0 0 2 1 1 0 0 2 3 1 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ttat gggttgaccgtcggtgccgaccac atgtactgggtccgccaggctccagggaaggggctgaagtgggtctcagtt ctttatggcggtggcaccttg gactacgcagactccgtgaagggccgattcaccatctccagagacaactcgaggaacactgtgtatcttcagatggagagactgagccccgaggacacggccgtctactactgt gcgagagaaagaggttcgggcccccccacaggcatggacgtc ARERGSGPPTGMDV IGHV3-53 IGHD3-10 IGHJ6 gggttgaccgtcggtgccgaccac atgtactgggtccgccaggctccagggaaggggctgaagtgggtctcagtt ctttatggcggtggcaccttg gactacgcagactccgtgaagggccgattcaccatctccagagacaactcgaggaacactgtgtatcttcagatggagagactgagccccgaggacacggccgtctactactgt gcgagagaaagaggttcgggcccccccacaggcatggacgtc IGG1 30 210 15 15 3 13 12 17 6 1 11 12 JY8QFUQ01A87BG 21748 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 959 93.58 204/218 nt NA Homsap IGHJ4*01 F, or Homsap IGHJ4*02 F or Homsap IGHJ4*03 F 168 83.33 40/48 nt Homsap IGHD3-22*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARGHSYYVSSGPDYW in-frame + cctctggattcacctttagcagctatgccatgaactgggtccgccaggctccagggaaggggctggagtgggtctcagttattagtggaagtggtgataccacatcgtacgcagactccgtgaagggccggttcaccatctcccgagacaattccaagaacacgatgtatctgcaaatgagtagcctgagagccgacgacacggccctttattactgtgcgagagggcacagttattatgtcagtagtgggcctgactactggggccacggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaaggcatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccag NA IGA2 79 89 [291, 293] g119>a,S40>N(- - -)|c164>t,A55>V(+ - +)|t174>a|g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)|a197>c,Y66>S(+ - -)|c198>g,Y66>S(+ - -)|a238>c|c259>a,L87>M(+ + -)|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|g291>c,E97>D(+ - +)|g301>c,V101>L(+ - +)|a303>t,V101>L(+ - +)|a317>g,K106>R(+ + +)| g119>a,S40>N(- - -)|c164>t,A55>V(+ - +)| t174>a|g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)| a197>c,Y66>S(+ - -)|c198>g,Y66>S(+ - -)|a238>c|c259>a,L87>M(+ + -)|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|g291>c,E97>D(+ - +)|g301>c,V101>L(+ - +)|a303>t,V101>L(+ - +)| a317>g,K106>R(+ + +)|a320>g| 319 (320) 225 (226) 210 15 (16) 2 (3) 13 2 (3) 2 2 0 2 1 1 1 3 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 0 1 0 0 0 0 0 1 1 0 0 0 117 114 105 9 1 8 1 0 1 0 2 1 1 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 1 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagcagctatgcc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcagtt attagtggaagtggtgataccaca tcgtacgcagactccgtgaagggccggttcaccatctcccgagacaattccaagaacacgatgtatctgcaaatgagtagcctgagagccgacgacacggccctttattactgt gcgagagggcacagttattatgtcagtagtgggcctgactac ARGHSYYVSSGPDY IGHV3-23 IGHD3-22 IGHJ4 ggattcacctttagcagctatgcc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcagtt attagtggaagtggtgataccaca tcgtacgcagactccgtgaagggccggttcaccatctcccgagacaattccaagaacacgatgtatctgcaaatgagtagcctgagagccgacgacacggccctttattactgt gcgagagggcacagttattatgtcagtagtgggcctgactac IGA2 14 213 5 9 4 9 1 5 1 1 10 2 JY8QFUQ01A87UU 21678 productive Homsap IGHV3-48*01 F 985 95.37 206/216 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 3 8 8 13 8.8.13 [1.17.38.11] CARERGNSYGEIDYW in-frame - tctggtttcaccttcaggagctctggcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagcagtagtactactaccaaatactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagaacgtggaaacagctatggagagattgactactggggccagggaaccctggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 93 88 [287] a81>t|t105>g,S35>R(- - -)|a110>c,Y37>S(+ - -)|a112>g,S38>G(+ + -)|t171>c|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t194>a,I65>K(- + -)|a200>g,Y67>C(- - -)|a254>g,N85>S(- - -)| a81>t|t105>g,S35>R(- - -)|a110>c,Y37>S(+ - -)|a112>g,S38>G(+ + -)| t171>c|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t194>a,I65>K(- + -)| a200>g,Y67>C(- - -)|a254>g,N85>S(- - -)| 320 224 214 10 2 8 3 0 0 1 1 0 1 1 2 0 0 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 0 0 1 0 1 0 0 0 0 1 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 0 0 0 1 0 0 0 1 2 0 0 0 117 114 112 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggtttcaccttcaggagctctggc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagcagtagtactactaccaaa tactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagaacgtggaaacagctatggagagattgactac ARERGNSYGEIDY IGHV3-48 IGHD5-18 IGHJ4 ggtttcaccttcaggagctctggc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagcagtagtactactaccaaa tactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagaacgtggaaacagctatggagagattgactac IGG2 10 213 4 6 0 2 4 8 0 2 2 6 JY8QFUQ01A8AJT 5353 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 851 88.07 192/218 nt 87.61 191/218 nt Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD5-18*01 F 3 8 8 10 8.8.10 [1.17.38.11] CMNEFGYCSGGG in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 89 of V-REGION: 'T' (1 nucleotides at position 171 to 171 in the user submitted sequence), (cause frameshift); gctctggattcacttttagcagccatatgatgacttgggtccgccaggctccagggaaggggctggaatgggtcgcaagtattcgtgccagtggtggtaggacacactacgcagactccgtgaagggccgcttcaccatctccagagacaactccaagaacacgttgtatTttgcaaatgtacagtctgagagtcgaggacacggccttatattattgtatgaacgaattcggatactgtagtggtgggggggcccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [280] c74>g|c87>t|t109>c,Y37>H(+ - -)|g112>a,A38>M(+ - -)|c113>t,A38>M(+ - -)|c114>g,A38>M(+ - -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g153>a|t160>g,S54>A(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|a169>c,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|c192>g,S64>R(- - -)|t196>c,Y66>H(+ - -)|g225>c|t246>c|c259>t|c265>t|a274>t,N92>Y(- - -)|c279>t|c287>t,A96>V(+ - +)|g301>t,V101>L(+ - +)|c309>t|g313>a,A105>M(+ - -)|c314>t,A105>M(+ - -)| c74>g| c87>t|t109>c,Y37>H(+ - -)|g112>a,A38>M(+ - -)|c113>t,A38>M(+ - -)|c114>g,A38>M(+ - -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g153>a|t160>g,S54>A(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| a169>c,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|c192>g,S64>R(- - -)| t196>c,Y66>H(+ - -)|g225>c|t246>c|c259>t|c265>t|a274>t,N92>Y(- - -)|c279>t|c287>t,A96>V(+ - +)|g301>t,V101>L(+ - +)|c309>t| g313>a,A105>M(+ - -)|c314>t,A105>M(+ - -)|a318>c,K106>N(+ - -)| 320 226 197 29 9 20 0 4 9 4 2 0 1 0 3 4 1 1 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 19 5 1 4 0 1 2 1 0 0 0 0 0 1 0 0 51 51 45 6 1 5 0 2 1 0 0 0 0 0 1 1 0 1 30 24 20 4 0 4 0 0 0 1 1 0 0 0 1 1 0 0 117 114 104 10 6 4 0 0 5 2 0 0 1 0 1 0 1 0 8 8 5 3 0 3 0 1 1 0 1 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gctct ggattcacttttagcagccatatg atgacttgggtccgccaggctccagggaaggggctggaatgggtcgcaagt attcgtgccagtggtggtaggaca cactacgcagactccgtgaagggccgcttcaccatctccagagacaactccaagaacacgttgtatttgcaaatgtacagtctgagagtcgaggacacggccttatattattgt atgaacgaattcggatactgtagtggtggg MNEFGYCSGG IGHV3-23 IGHD5-18 IGHJ4 ggattcacttttagcagccatatg atgacttgggtccgccaggctccagggaaggggctggaatgggtcgcaagt attcgtgccagtggtggtaggaca cactacgcagactccgtgaagggccgcttcaccatctccagagacaactccaagaacacgttgtatttgcaaatgtacagtctgagagtcgaggacacggccttatattattgt atgaacgaattcggatactgtagtggtggg IGA1 25 213 15 10 11 18 4 7 7 1 9 8 JY8QFUQ01A8ALV 3891 productive Homsap IGHV3-48*02 F 967 94.44 204/216 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-22*01 F 2 8 8 14 8.8.14 [1.17.38.11] CAKDRGGSAYPIFDYW in-frame + tctggattcaccttcggcagttataggatgagctgggtccgccaggctccagggaaggggctggagtggatttcatacattagtagtagtagtagtgccatctattacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcagtgtatctgcaaatgaacagcctgagagacgaggacacggctatatattactgtgcgaaagataggggcggtagtgcttatcccatctttgactactggggccagggatccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 89 [289, 288] a103>g,S35>G(+ + -)|t105>c,S35>G(+ + -)|c108>t|c114>g,S38>R(- - -)|a119>g,N40>S(- - -)|g157>a,V53>I(+ - +)|a190>g,T64>A(- - -)|a195>c|c198>t|c259>g,L87>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|g317>a,R106>K(+ + +)| a103>g,S35>G(+ + -)|t105>c,S35>G(+ + -)|c108>t|c114>g,S38>R(- - -)| a119>g,N40>S(- - -)|g157>a,V53>I(+ - +)| a190>g,T64>A(- - -)|a195>c| c198>t|c259>g,L87>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g317>a,R106>K(+ + +)| 320 224 211 13 3 10 3 4 2 1 1 0 0 0 0 2 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 1 1 0 0 0 0 0 1 0 0 51 51 49 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 30 24 22 2 1 1 1 0 0 0 1 0 0 0 0 0 0 0 117 114 110 4 1 3 0 2 1 0 0 0 0 0 0 1 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| tct ggattcaccttcggcagttatagg atgagctgggtccgccaggctccagggaaggggctggagtggatttcatac attagtagtagtagtagtgccatc tattacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcagtgtatctgcaaatgaacagcctgagagacgaggacacggctatatattactgt gcgaaagataggggcggtagtgcttatcccatctttgactac AKDRGGSAYPIFDY IGHV3-48 IGHD3-22 IGHJ4 ggattcaccttcggcagttatagg atgagctgggtccgccaggctccagggaaggggctggagtggatttcatac attagtagtagtagtagtgccatc tattacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcagtgtatctgcaaatgaacagcctgagagacgaggacacggctatatattactgt gcgaaagataggggcggtagtgcttatcccatctttgactac IGA2 12 213 9 3 5 7 4 5 1 2 5 4 JY8QFUQ01A8BTW 17700 productive Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F or Homsap IGHV4-34*12 F (see comment) 701 80.93 174/215 nt NA Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD3-3*01 F 3 8 7 19 8.7.19 [1.17.38.11] CARAYTFSLFGVASHWYFDLW in-frame + Low V-REGION identity (80,93% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tctctgatgggtcgttcatgggttacctctggaattggatccgccagcccccagggaaggggctggagtggattggggaaatcagtcctagcggcgtcagtaagtacaatacgtccctcaagagtcgcgttgttatgagaatggacacgtcgaagaagcaattctccctggagatcaactctgtgaccgccgcggacacggctacttattattgtgcgagagcctatactttttcactttttggagtggcctcacactggtacttcgatctctggggccgtggcaccctggtcacagtctcctcagcatccccggaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 95 [304] a77>c,Y26>S(+ - -)|g80>a,G27>D(- - -)|c87>g|g104>t,S35>M(- - -)|t105>g,S35>M(- - -)|t112>c,Y38>L(- - -)|a113>t,Y38>L(- - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a170>g,N57>S(- - -)|a173>c,H58>P(+ - -)|t177>c|a189>c|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|c194>g,T65>S(+ - +)|c195>t,T65>S(+ - +)|c198>g,N66>K(+ - -)|c204>t|c205>a,P69>T(+ + -)|a225>c|c228>t|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|c231>t,T77>V(- - -)|a234>g,I78>M(+ + -)|t235>a,S79>R(- - -)|c236>g,S79>R(- - -)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|c249>g|c255>g,N85>K(+ - -)|g258>a|a268>g,K90>E(+ - -)|c271>a,L91>I(+ + +)|g273>c,L91>I(+ + +)|g275>a,S92>N(- - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|c309>t| a77>c,Y26>S(+ - -)| g80>a,G27>D(- - -)|c87>g|g104>t,S35>M(- - -)|t105>g,S35>M(- - -)|t112>c,Y38>L(- - -)|a113>t,Y38>L(- - -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)| a170>g,N57>S(- - -)|a173>c,H58>P(+ - -)|t177>c|a189>c|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|c194>g,T65>S(+ - +)|c195>t,T65>S(+ - +)| c198>g,N66>K(+ - -)|c204>t|c205>a,P69>T(+ + -)|a225>c|c228>t|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|c231>t,T77>V(- - -)|a234>g,I78>M(+ + -)|t235>a,S79>R(- - -)|c236>g,S79>R(- - -)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|c249>g|c255>g,N85>K(+ - -)|g258>a|a268>g,K90>E(+ - -)|c271>a,L91>I(+ + +)|g273>c,L91>I(+ + +)|g275>a,S92>N(- - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|c309>t| g320>c| 319 (320) 222 (223) 181 41 (42) 9 (10) 32 6 6 7 3 4 2 1 1 1 (2) 6 3 1 78 5 4 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 36 24 18 6 1 5 0 1 0 1 0 0 1 0 0 1 1 1 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 21 13 8 2 6 2 0 1 1 2 0 0 0 0 1 1 0 117 114 90 24 6 18 4 4 5 1 1 2 0 1 1 4 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct gatgggtcgttcatgggttacctc tggaattggatccgccagcccccagggaaggggctggagtggattggggaa atcagtcctagcggcgtcagt aagtacaatacgtccctcaagagtcgcgttgttatgagaatggacacgtcgaagaagcaattctccctggagatcaactctgtgaccgccgcggacacggctacttattattgt gcgagagcctatactttttcactttttggagtggcctcacactggtacttcgatctc ARAYTFSLFGVASHWYFDL IGHV4-34 IGHD3-3 IGHJ2 gatgggtcgttcatgggttacctc tggaattggatccgccagcccccagggaaggggctggagtggattggggaa atcagtcctagcggcgtcagt aagtacaatacgtccctcaagagtcgcgttgttatgagaatggacacgtcgaagaagcaattctccctggagatcaactctgtgaccgccgcggacacggctacttattattgt gcgagagcctatactttttcactttttggagtggcctcacactggtacttcgatctc IGA2 40 210 22 18 13 25 9 15 6 3 20 11 JY8QFUQ01A8CRV 9673 productive Homsap IGHV3-48*01 F 864 88.94 193/217 nt NA Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD3-22*01 F 2 8 8 19 8.8.19 [1.17.38.11] CARASTFYYDGSGYRDVFDIW in-frame + ctctggcttcagtttgagtacttataccatgaactgggtccgccaggctccagggaaggggctggagtgggtttcactcattagtaagactagtaatgtcatatactacgcggactctgtgaagggccggttcaccatctccagagacaatgccgagaattcactgtttctgcaaatggacagcctgagtgccgaggacacgggtgtatattactgtgcgagagcttcgacgttctactacgatggcagtggttatcgtgatgtttttgatatatggggccaagggacaatggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 94 [305, 306] a81>c|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g113>c,S38>T(+ - +)|t163>c,Y55>L(- - -)|a164>t,Y55>L(- - -)|g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g176>c,S59>T(+ - +)|g188>a,S63>N(- - -)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)|a204>g|a225>g|a250>g,K84>E(+ - -)|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|a285>t,R95>S(- - -)|c299>g,A100>G(- + -)|g303>a| a81>c|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g113>c,S38>T(+ - +)| t163>c,Y55>L(- - -)|a164>t,Y55>L(- - -)| g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g176>c,S59>T(+ - +)|g188>a,S63>N(- - -)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)| a204>g|a225>g|a250>g,K84>E(+ - -)|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|a285>t,R95>S(- - -)|c299>g,A100>G(- + -)|g303>a| a320>c| 319 (320) 224 (225) 200 24 (25) 5 (6) 19 5 3 4 1 1 (2) 0 3 0 3 3 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 1 6 0 0 2 0 1 0 0 0 2 2 0 0 51 51 49 2 0 2 0 0 0 1 0 0 1 0 0 0 0 0 30 24 18 6 0 6 1 2 1 0 0 0 0 0 1 0 0 1 117 114 105 9 4 5 4 1 1 0 0 0 2 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggcttcagtttgagtacttatacc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcactc attagtaagactagtaatgtcata tactacgcggactctgtgaagggccggttcaccatctccagagacaatgccgagaattcactgtttctgcaaatggacagcctgagtgccgaggacacgggtgtatattactgt gcgagagcttcgacgttctactacgatggcagtggttatcgtgatgtttttgatata ARASTFYYDGSGYRDVFDI IGHV3-48 IGHD3-22 IGHJ3 ggcttcagtttgagtacttatacc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcactc attagtaagactagtaatgtcata tactacgcggactctgtgaagggccggttcaccatctccagagacaatgccgagaattcactgtttctgcaaatggacagcctgagtgccgaggacacgggtgtatattactgt gcgagagcttcgacgttctactacgatggcagtggttatcgtgatgtttttgatata IGG1 24 213 13 11 7 13 6 11 4 1 7 12 JY8QFUQ01A8FGD 20740 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 923 91.28 199/218 nt 90.83 198/218 nt Homsap IGHJ1*01 F (see comment) 133 74 37/50 nt Homsap IGHD5-18*01 F 3 8 8 6 8.8.6 [1.17.38.11] CASYGQWL in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 55 of V-REGION: 'AGT' (3 nucleotides at position 78 to 80 in the user submitted sequence), (do not cause frameshift); cctcgggactcatgtttagcagctatgccatgacctgggtccgccaggctccagggaaggggctggagtgggtctcaAGTgtcagtagtagtactggttatttcacatactacacagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtatattattgcgctagttacgggcagtggctgggccagggaacccaggtcaccgtctcctcagcttccaccaagggcccatccgtcttcccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG4 100 88 [272, 271] t78>g|t82>c,F28>L(+ - -)|c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|g119>c,S40>T(+ - +)|c164>t,A55>V(+ - +)|t165>c,A55>V(+ - +)|t167>g,I56>S(- - -)|g172>a,G58>S(+ + -)|g176>c,S59>T(+ - +)|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)|g202>a,A68>T(- - -)|a275>g,N92>S(- - -)|g291>a|c309>t|t312>c| t78>g| t82>c,F28>L(+ - -)|c86>t,T29>M(- - -)|c87>g,T29>M(- - -)| g119>c,S40>T(+ - +)|c164>t,A55>V(+ - +)|t165>c,A55>V(+ - +)| t167>g,I56>S(- - -)|g172>a,G58>S(+ + -)|g176>c,S59>T(+ - +)|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)| g202>a,A68>T(- - -)|a275>g,N92>S(- - -)|g291>a|c309>t|t312>c| g315>t|a317>g,K106>S(- - -)|a318>t,K106>S(- - -)|g319>t| 314 (320) 220 (226) 201 (203) 19 (23) 4 (6) 15 (17) 1 (2) 4 3 3 0 0 1 (2) 0 2 1 2 (4) 2 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 36 24 21 3 0 3 0 0 1 1 0 0 0 0 0 1 0 0 51 51 48 3 0 3 0 0 1 1 0 0 0 0 1 0 0 0 30 24 17 7 0 7 0 2 0 0 0 0 1 0 1 0 2 1 117 114 109 5 3 2 1 2 1 1 0 0 0 0 0 0 0 0 2 (8) 2 2 (4) 0 (4) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (2) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctcg ggactcatgtttagcagctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcagtc agtagtagtactggttatttcaca tactacacagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtatattattgc gctagttacgggcagtgg ASYGQW IGHV3-23 IGHD5-18 IGHJ1 ggactcatgtttagcagctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcagtc agtagtagtactggttatttcaca tactacacagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtatattattgc gctagttacgggcagtgg IGG4 18 213 11 7 7 12 4 6 3 0 5 10 JY8QFUQ01A8FJO 16272 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 993 96.26 206/214 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD1-26*01 F 1 8 8 13 8.8.13 [0.17.38.11] CARPGVGASSFIDFW in-frame + tggatacagctttaccgcctactggatcggctgggtgcgccagatgcccgggaaaggcctggagtgggtggcgatcatctatcctggtgactctgaaaccagatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcaccaccgcctacctgcattggaccagcctgaaggcctcggacaccgccatgtattactgtgcgagaccaggagtgggggcctcttctttcattgacttctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a157>g,M53>V(+ - -)|g161>c,G54>A(- + -)|t192>a,D64>E(+ - +)|g254>c,S85>T(+ - +)|g270>t,Q90>H(- + -)|g275>c,S92>T(+ - +)| a106>g,S36>A(- + -)|g107>c,S36>A(- + -)| a157>g,M53>V(+ - -)|g161>c,G54>A(- + -)| t192>a,D64>E(+ - +)| g254>c,S85>T(+ - +)|g270>t,Q90>H(- + -)|g275>c,S92>T(+ - +)| a320>c| 319 (320) 221 (222) 213 8 (9) 0 (1) 8 2 0 0 0 0 (1) 0 0 1 4 0 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 51 51 49 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 30 24 23 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 117 114 111 3 0 3 0 0 0 0 0 0 0 0 2 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacagctttaccgcctactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtgggtggcgatc atctatcctggtgactctgaaacc agatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcaccaccgcctacctgcattggaccagcctgaaggcctcggacaccgccatgtattactgt gcgagaccaggagtgggggcctcttctttcattgacttc ARPGVGASSFIDF IGHV5-51 IGHD1-26 IGHJ4 ggatacagctttaccgcctactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtgggtggcgatc atctatcctggtgactctgaaacc agatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcaccaccgcctacctgcattggaccagcctgaaggcctcggacaccgccatgtattactgt gcgagaccaggagtgggggcctcttctttcattgacttc IGA1 8 213 2 6 0 5 2 3 0 0 5 3 JY8QFUQ01A8FM1 19382 productive (see comment) Homsap IGHV1-24*01 F 957 94.39 202/214 nt 93.93 201/214 nt Homsap IGHJ1*01 F (see comment) 161 78.85 41/52 nt Homsap IGHD3-16*01 F 2 8 8 11 8.8.11 [0.17.38.11] CVGDVGWGYPQGW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 55 of V-REGION: 'A' (1 nucleotides at position 76 to 76 in the user submitted sequence), (cause frameshift); cggatacaccctcaatgaattatccatgcactgggtgcgacagactcctggaaaagggcttgagtcgatgggcggAttttgatcctgaatatggtaaaacaatctacgcgcagaagttccagggcagagtcaccatgaccgaggacacatctgcagacacagcctacatggagttgagaagcctgagatttgaggacacggccgtgtattattgtgtcggagatgtcggctggggatacccccaaggctggggccagggaaccctggtcaccgtctccgcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [279] c104>a,T35>N(- + -)|g133>a,A45>T(- - -)|g155>c,W52>S(- - -)|a162>c|g184>t,D62>Y(- - -)|g190>a,E64>K(+ - -)|a204>g|a250>g,T84>A(- - -)|c271>t|c276>a,S92>R(- - -)|c287>t,S96>F(- - -)|c309>t| c104>a,T35>N(- + -)| g133>a,A45>T(- - -)|g155>c,W52>S(- - -)|a162>c| g184>t,D62>Y(- - -)|g190>a,E64>K(+ - -)| a204>g|a250>g,T84>A(- - -)|c271>t|c276>a,S92>R(- - -)|c287>t,S96>F(- - -)|c309>t| c314>t,A105>V(+ - +)|a315>c,A105>V(+ - +)|a316>g,T106>G(+ - -)|c317>g,T106>G(+ - -)| 320 222 206 16 4 12 3 2 4 0 2 2 0 0 1 1 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 48 3 1 2 0 1 0 0 1 0 0 0 1 0 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 0 0 1 0 117 114 108 6 3 3 2 0 3 0 0 1 0 0 0 0 0 0 8 8 4 4 0 4 1 0 1 0 1 0 0 0 0 1 0 0 ta,14-15(FR1)|aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|aa,107-108(CDR1)|ta,110-111(CDR1)|aa,141-142(FR2)|aa,143-144(FR2)|aa,176-177(CDR2)|aa,191-192(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,249-250(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,14-15(FR1)|tt,74-75(FR1)|ta,82-83(CDR1)|tt,109-110(CDR1)|tt,149-150(FR2)|tt,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,249-250(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggta,12-15(FR1)|ggct,24-27(FR1)|ggtt,72-75(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,163-166(FR2-CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| c ggatacaccctcaatgaattatcc atgcactgggtgcgacagactcctggaaaagggcttgagtcgatgggcggt tttgatcctgaatatggtaaaaca atctacgcgcagaagttccagggcagagtcaccatgaccgaggacacatctgcagacacagcctacatggagttgagaagcctgagatttgaggacacggccgtgtattattgt gtcggagatgtcggctggggatacccccaaggc VGDVGWGYPQG IGHV1-24 IGHD3-16 IGHJ1 ggatacaccctcaatgaattatcc atgcactgggtgcgacagactcctggaaaagggcttgagtcgatgggcggt tttgatcctgaatatggtaaaaca atctacgcgcagaagttccagggcagagtcaccatgaccgaggacacatctgcagacacagcctacatggagttgagaagcctgagatttgaggacacggccgtgtattattgt gtcggagatgtcggctggggatacccccaaggc IGA1 12 213 7 5 5 9 2 3 4 0 5 3 JY8QFUQ01A8FSV 21274 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F or Homsap IGHV3-21*04 F 813 86.92 186/214 nt 86.45 185/214 nt Homsap IGHJ3*02 F 178 84 42/50 nt Homsap IGHD6-19*01 F 1 8 8 14 8.8.14 [0.17.38.11] CARGFSAWGQDAFDIW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 41 of V-REGION: 'G' (1 nucleotides at position 33 to 33 in the user submitted sequence), (cause frameshift); tggatttatcttcagtgattatagcatgaattGgggtccgccagactccagggaaggggctggagtgggtctcttccattagtagtcgaagtactttcgaatattacgcggactcagtgaagggccgattcaccatctccagagacaactccaataactcagtgtatctgcagatgaacagggtgacagccgacgacgcggcagtctatttctgtgccagaggcttcagtgcctggggtcaggatgctttcgacatttggggccacgggacctttgtcaccgtctcttcagcctccaccaagggcccatcggtcttcccccctggcaccctcctccaagagcacctctggggggcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [290, 289] c84>t|c86>t,T29>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|c120>t|g133>a,A45>T(- - -)|a162>t|a175>c,S59>R(- - -)|t177>a,S59>R(- - -)|g188>c,S63>T(+ - +)|a191>t,Y64>F(- + -)|a193>g,I65>E(- - -)|t194>a,I65>E(- - -)|c198>t|a204>g|g247>t,A83>S(- + -)|g252>t,K84>N(+ - -)|c259>g,L87>V(+ - +)|a270>g|c279>g,S93>R(- - -)|c280>g,L94>V(+ - +)|g284>c,R95>T(- - -)|g291>c,E97>D(+ - +)|a295>g,T99>A(- - -)|t300>a|g303>c|a308>t,Y103>F(- + -)|g315>c| c84>t|c86>t,T29>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)| c120>t|g133>a,A45>T(- - -)|a162>t| a175>c,S59>R(- - -)|t177>a,S59>R(- - -)|g188>c,S63>T(+ - +)|a191>t,Y64>F(- + -)|a193>g,I65>E(- - -)|t194>a,I65>E(- - -)| c198>t|a204>g|g247>t,A83>S(- + -)|g252>t,K84>N(+ - -)|c259>g,L87>V(+ - +)|a270>g|c279>g,S93>R(- - -)|c280>g,L94>V(+ - +)|g284>c,R95>T(- - -)|g291>c,E97>D(+ - +)|a295>g,T99>A(- - -)|t300>a|g303>c|a308>t,Y103>F(- + -)| g315>c|a320>g| 319 (320) 221 (222) 192 29 (30) 9 (10) 20 5 (6) 2 5 0 1 0 3 3 5 3 2 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 1 1 3 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 0 0 1 0 0 0 0 0 30 24 18 6 0 6 1 0 0 0 1 0 1 2 1 0 0 0 117 114 100 14 5 9 3 0 1 0 0 0 1 1 3 3 2 0 7 (8) 7 6 1 (2) 1 (2) 0 0 (1) 0 0 0 0 0 0 0 1 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t ggatttatcttcagtgattatagc atgaattgggtccgccagactccagggaaggggctggagtgggtctcttcc attagtagtcgaagtactttcgaa tattacgcggactcagtgaagggccgattcaccatctccagagacaactccaataactcagtgtatctgcagatgaacagggtgacagccgacgacgcggcagtctatttctgt gccagaggcttcagtgcctggggtcaggatgctttcgacatt ARGFSAWGQDAFDI IGHV3-21 IGHD6-19 IGHJ3 ggatttatcttcagtgattatagc atgaattgggtccgccagactccagggaaggggctggagtgggtctcttcc attagtagtcgaagtactttcgaa tattacgcggactcagtgaagggccgattcaccatctccagagacaactccaataactcagtgtatctgcagatgaacagggtgacagccgacgacgcggcagtctatttctgt gccagaggcttcagtgcctggggtcaggatgctttcgacatt IGG1 28 213 12 16 7 16 5 12 7 1 10 10 JY8QFUQ01A8G0E 8245 productive (see comment) Homsap IGHV3-11*01 F 904 90.74 196/216 nt 90.28 195/216 nt Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD6-19*01 F 1 8 8 16 8.8.16 [1.17.37.11] CARRLLSSGWPPHEWGFW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 84 of V-REGION: 1 nucleotides (from position 157 in the user submitted sequence), (cause frameshift) ctctggattcaccttcagtgactactacatgagctggatccgccaggctccagggaagggactggagtgggtctcatatattagcagtactggtggttccatatattacgcagactctgtgaagggccgcttcaccacctccagggacaacaccaaaactcaatgtctctgcaaatgaacagcctgagcgtcgacgacacgggcgtctattattgcgcgagaaggcttctcagtagtggatggccaccccacgagtggggcttctggggccagggagtccaggtcaccgtctcctcaccatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccca NA IGA1 100 100 [295] g147>a|t159>c|c165>t|t171>c|g176>c,S59>T(+ - +)|a187>g,S63>G(+ + -)|a190>t,T64>S(+ - +)|c198>t|a225>c|t233>c,I78>T(- - -)|g247>a,A83>T(- - -)|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|a285>c,R95>S(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c299>g,A100>G(- + -)|g303>c|c309>t|t312>c| g147>a|t159>c|c165>t| t171>c|g176>c,S59>T(+ - +)|a187>g,S63>G(+ + -)|a190>t,T64>S(+ - +)| c198>t|a225>c|t233>c,I78>T(- - -)|g247>a,A83>T(- - -)|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|a285>c,R95>S(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c299>g,A100>G(- + -)|g303>c|c309>t|t312>c| g319>a|a320>g| 318 (320) 222 (224) 202 20 (22) 9 (11) 11 1 (2) 2 (3) 4 4 3 1 1 0 3 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 48 3 3 0 0 1 1 1 0 0 0 0 0 0 0 0 30 24 20 4 1 3 1 0 0 1 0 0 1 0 1 0 0 0 117 113 100 13 5 8 0 1 3 2 3 1 0 0 2 1 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgactactac atgagctggatccgccaggctccagggaagggactggagtgggtctcatat attagcagtactggtggttccata tattacgcagactctgtgaagggccgcttcaccacctccagggacaacaccaaaactcaatgtctctgcaaatgaacagcctgagcgtcgacgacacgggcgtctattattgc gcgagaaggcttctcagtagtggatggccaccccacgagtggggcttc ARRLLSSGWPPHEWGF IGHV3-11 IGHD6-19 IGHJ4 ggattcaccttcagtgactactac atgagctggatccgccaggctccagggaagggactggagtgggtctcatat attagcagtactggtggttccata tattacgcagactctgtgaagggccgcttcaccacctccagggacaacaccaaaactcaatgtctctgcaaatgaacagcctgagcgtcgacgacacgggcgtctattattgc gcgagaaggcttctcagtagtggatggccaccccacgagtggggcttc IGA1 20 212 11 9 6 11 5 9 8 1 8 3 JY8QFUQ01A8GGU 22237 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 954 93.55 203/217 nt 93.09 202/217 nt Homsap IGHJ2*01 F 247 96.23 51/53 nt Homsap IGHD2-21*01 F 1 8 8 12 8.8.12 [1.16.38.11] CAKVVWSLWYFDLW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 59 in the user submitted sequence), (cause frameshift) cgtctacattcgcctttagcaactatgccatgagctgggtccgccaggctccagggaagggctggagtgggtgtcagatattagtgggagtggtgttagcacacactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgtgcgaaagtagtctggagcctctggtacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [285] c75>g|g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)|c159>g|c164>a,A55>D(- - -)|t174>g|g188>t,G63>V(- - -)|t196>c,Y66>H(+ - -)|a204>g|a214>c,K72>Q(+ - -)|c249>g|a285>t,R95>S(- - -)| c75>g| g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)| c159>g|c164>a,A55>D(- - -)| t174>g|g188>t,G63>V(- - -)| t196>c,Y66>H(+ - -)|a204>g|a214>c,K72>Q(+ - -)|c249>g|a285>t,R95>S(- - -)| a320>t| 319 (320) 224 (225) 210 14 (15) 5 (6) 9 2 2 0 1 1 1 1 (2) 0 1 3 1 1 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 20 4 0 4 1 2 0 0 0 0 0 0 1 0 0 0 51 50 48 2 1 1 0 0 0 0 0 1 0 0 0 1 0 0 30 24 22 2 1 1 0 0 0 0 0 0 0 0 0 0 1 1 117 114 109 5 2 3 1 0 0 1 1 0 1 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cgtct acattcgcctttagcaactatgcc atgagctgggtccgccaggctccagggaagggctggagtgggtgtcagat attagtgggagtggtgttagcaca cactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgt gcgaaagtagtctggagcctctggtacttcgatctc AKVVWSLWYFDL IGHV3-23 IGHD2-21 IGHJ2 acattcgcctttagcaactatgcc atgagctgggtccgccaggctccagggaagggctggagtgggtgtcagat attagtgggagtggtgttagcaca cactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgt gcgaaagtagtctggagcctctggtacttcgatctc IGG1 13 212 5 8 2 7 3 6 3 1 4 5 JY8QFUQ01A8GWB 16369 productive Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F 850 88.43 191/216 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-16*02 F 1 8 7 13 8.7.13 [2.17.38.11] CTRGRSDAWELSDHW in-frame - gtcgacgatgggtcctgcagaaactgcttctggagttggatccgccagtccccagggaaggggctggagtggattggggaggtcaatgatagaggaggcatcgactacaacccgtccctcaagagtcgagtcaccatatcattagacacgtccaacaaccaagtctccctgaggttgagctctgtgaccgccgcggacacggctgtgtattactgtacgcgagggcgatccgacgcgtgggaattatcggaccactggggccagggaaccgtggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcc NA IGA2 100 100 [286] t76>g,Y26>D(- - -)|t78>c,Y26>D(- - -)|g80>a,G27>D(- - -)|t89>g,F30>C(+ - -)|t105>a,S35>R(- - -)|g106>a,G36>N(- - -)|g107>a,G36>N(- - -)|t108>c,G36>N(- - -)|a110>g,Y37>C(- - -)|a113>t,Y38>F(- + -)|c120>t|c133>t,P45>S(+ - -)|a165>g|a166>g,I56>V(+ - +)|c172>g,H58>D(- - -)|t177>a,S59>R(- - -)|a190>g,S64>G(+ + -)|c194>t,T65>I(- - -)|a196>g,N66>D(+ + -)|g238>t,V80>L(+ - +)|g252>c,K84>N(+ - -)|g258>a|t259>g,F87>V(+ - -)|a269>g,K90>R(+ + +)|c271>t|g313>a,A105>T(- - -)|a316>c| t76>g,Y26>D(- - -)|t78>c,Y26>D(- - -)| g80>a,G27>D(- - -)|t89>g,F30>C(+ - -)|t105>a,S35>R(- - -)|g106>a,G36>N(- - -)|g107>a,G36>N(- - -)|t108>c,G36>N(- - -)|a110>g,Y37>C(- - -)|a113>t,Y38>F(- + -)| c120>t|c133>t,P45>S(+ - -)|a165>g| a166>g,I56>V(+ - +)|c172>g,H58>D(- - -)|t177>a,S59>R(- - -)|a190>g,S64>G(+ + -)|c194>t,T65>I(- - -)| a196>g,N66>D(+ + -)|g238>t,V80>L(+ - +)|g252>c,K84>N(+ - -)|g258>a|t259>g,F87>V(+ - -)|a269>g,K90>R(+ + +)|c271>t| g313>a,A105>T(- - -)|a316>c| 320 224 197 27 5 22 6 5 4 2 1 0 1 2 1 1 1 3 78 6 4 2 0 2 0 0 0 1 0 0 0 0 0 0 0 1 36 24 16 8 0 8 1 3 0 1 0 0 1 1 0 0 0 1 51 51 48 3 2 1 1 0 2 0 0 0 0 0 0 0 0 0 30 21 16 5 0 5 2 0 1 0 0 0 0 1 0 1 0 0 117 114 107 7 2 5 2 1 1 0 0 0 0 0 1 0 1 1 8 8 6 2 1 1 0 1 0 0 1 0 0 0 0 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtcgac gatgggtcctgcagaaactgcttc tggagttggatccgccagtccccagggaaggggctggagtggattggggag gtcaatgatagaggaggcatc gactacaacccgtccctcaagagtcgagtcaccatatcattagacacgtccaacaaccaagtctccctgaggttgagctctgtgaccgccgcggacacggctgtgtattactgt acgcgagggcgatccgacgcgtgggaattatcggaccac TRGRSDAWELSDH IGHV4-34 IGHD3-16 IGHJ4 gatgggtcctgcagaaactgcttc tggagttggatccgccagtccccagggaaggggctggagtggattggggag gtcaatgatagaggaggcatc gactacaacccgtccctcaagagtcgagtcaccatatcattagacacgtccaacaaccaagtctccctgaggttgagctctgtgaccgccgcggacacggctgtgtattactgt acgcgagggcgatccgacgcgtgggaattatcggaccac IGA2 23 210 15 8 8 11 7 12 4 0 6 13 JY8QFUQ01A8JNL 14488 productive (see comment) Homsap IGHV2-5*02 F 1071 93.44 228/244 nt 93.03 227/244 nt Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 187 86 43/50 nt Homsap IGHD2-21*02 F 3 10 7 13 10.7.13 [9.17.38.11] CAHRRVGTATAFDFW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 56 of V-REGION: 'C' (1 nucleotides at position 111 to 111 in the user submitted sequence), (cause frameshift); gaccctcacgctgacctgcaccttctctgggttctcactcagtaacagtggagtgggtgtgggctgggtccgtcagcccccaggaaaggccctggagtggcttggacacaCttttttgggatgatgataagcgctacagtccctctctgaaaagcaggctcaccctcaccaaggatacctccaaagaccaggtggtccttgaaatgaccaacatggaccctgtggacacagccacttattactgtgctcacagacgtgtggggactgcgaccgcttttgatttctggggccaggggacaacggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [315] c93>t|c101>a,T34>N(- + -)|t102>c,T34>N(- + -)|a124>g,I42>V(+ - +)|c161>g,A54>G(- + -)|t164>a,L55>H(- - -)|a170>t,Y57>F(- + -)|c204>t|a207>c|g216>a|a232>c,I78>L(+ + +)|c243>t|a253>g,N85>D(+ + -)|a268>g,T90>E(- - -)|c269>a,T90>E(- - -)|a303>t|a315>t| c93>t|c101>a,T34>N(- + -)|t102>c,T34>N(- + -)| a124>g,I42>V(+ - +)|c161>g,A54>G(- + -)|t164>a,L55>H(- - -)| a170>t,Y57>F(- + -)| c204>t|a207>c|g216>a|a232>c,I78>L(+ + +)|c243>t|a253>g,N85>D(+ + -)|a268>g,T90>E(- - -)|c269>a,T90>E(- - -)|a303>t| a315>t| 322 254 237 17 7 10 3 1 3 1 2 2 3 1 0 1 0 0 78 28 28 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 1 2 0 0 1 1 0 1 0 0 0 0 0 0 51 51 48 3 0 3 1 0 0 0 0 0 0 1 0 1 0 0 30 21 20 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 117 114 105 9 5 4 2 1 2 0 2 1 1 0 0 0 0 0 10 10 9 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 aa,13-14(FR1)|aa,40-41(FR1)|ta,102-103(CDR1)|aa,141-142(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,238-239(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,267-268(FR3)|aa,270-271(FR3)|aa,277-278(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,10-11(FR1)|tt,73-74(FR1)|tt,82-83(CDR1)|ta,102-103(CDR1)|tt,158-159(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,80-83(CDR1)|ggct,118-121(FR2)|ggct,155-158(FR2)|agca,220-223(FR3)|ggct,224-227(FR3)| aacc,41-44(FR1)|agcc,131-134(FR2)|agcc,202-205(FR3)|aacc,253-256(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)| gaccctcacgctgacctgcaccttctct gggttctcactcagtaacagtggagtgggt gtgggctgggtccgtcagcccccaggaaaggccctggagtggcttggacac attttttgggatgatgataag cgctacagtccctctctgaaaagcaggctcaccctcaccaaggatacctccaaagaccaggtggtccttgaaatgaccaacatggaccctgtggacacagccacttattactgt gctcacagacgtgtggggactgcgaccgcttttgatttc AHRRVGTATAFDF IGHV2-5 IGHD2-21 IGHJ3 gggttctcactcagtaacagtggagtgggt gtgggctgggtccgtcagcccccaggaaaggccctggagtggcttggacac attttttgggatgatgataag cgctacagtccctctctgaaaagcaggctcaccctcaccaaggatacctccaaagaccaggtggtccttgaaatgaccaacatggaccctgtggacacagccacttattactgt gctcacagacgtgtggggactgcgaccgcttttgatttc IGA1 16 216 8 8 4 7 4 9 5 1 7 3 JY8QFUQ01A8JZF 17807 productive Homsap IGHV3-7*01 F 968 94.04 205/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSGFYSTGYW in-frame + gctctggattcagctttagttattattggatgagctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaaggaagatggaagtgagagacactatgtggactctgtgatgggccggttcaccatctccagagacaacgccaagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgtgcgattagtagtggtttttactcgacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcatcgcctgcctggtccagggcttcttcc NA IGA2 71 100 [282] c74>g|c86>g,T29>S(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c108>t,S36>Y(+ - -)|c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)|t196>c,Y66>H(+ - -)|a215>t,K72>M(- + -)|a225>g|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)|g303>a| c74>g| c86>g,T29>S(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c108>t,S36>Y(+ - -)| c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)| t196>c,Y66>H(+ - -)|a215>t,K72>M(- + -)|a225>g|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)|g303>a| g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 316 (320) 222 (226) 209 13 (17) 3 (5) 10 (12) 3 (4) 2 (3) 1 1 0 0 2 (3) 0 0 4 0 (1) 0 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 20 4 0 4 0 1 1 0 0 0 1 0 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 1 0 0 117 114 108 6 2 4 2 1 0 1 0 0 1 0 0 1 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gctct ggattcagctttagttattattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagaga cactatgtggactctgtgatgggccggttcaccatctccagagacaacgccaagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgattagtagtggtttttactcgacgggctac AISSGFYSTGY IGHV3-7 IGHD3-22 IGHJ4 ggattcagctttagttattattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagaga cactatgtggactctgtgatgggccggttcaccatctccagagacaacgccaagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgattagtagtggtttttactcgacgggctac IGA2 12 213 7 5 3 6 4 6 2 0 4 6 JY8QFUQ01A8KHD 8665 productive Homsap IGHV3-9*01 F (see comment) 793 84.93 186/219 nt NA Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 151 78 39/50 nt Homsap IGHD6-19*01 F 2 8 8 13 8.8.13 [2.17.38.11] CGRIAKPGLGALDLW in-frame - Low V-REGION identity (84,93% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ggcgttggattcacttttaatgaacatggcatgcagtgggtccggcaagctccagggaagggcctggagtgggtcgcaggtatcagcggtaatggtgatgtcataggatatgcggactctgtgaagggccgagtcaccgtctccagagacaacgccaaagactctctatatttgcagatggacagtctgagagttaatgacacggccttatattattgtggccgcatagcgaagcctggtctgggcgccttagatctctggggccgtggaacaatggtcaccgtctctgaagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [289] c74>g,A25>G(- + -)|t76>g,S26>V(- - -)|c77>t,S26>V(- - -)|c87>t|g103>a,D35>N(+ + -)|t108>a,D36>E(+ - +)|t109>c,Y37>H(+ - -)|c113>g,A38>G(- + -)|c120>g,H40>Q(- + -)|t160>g,S54>A(- + -)|t168>c|t171>c|t172>g,W58>G(- - -)|g174>t,W58>G(- - -)|a184>g,S62>G(+ + -)|g188>a,G63>D(- - -)|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|c198>a|t226>g,F76>V(+ - -)|a232>g,I78>V(+ - +)|g252>a|a253>g,N85>D(+ + -)|c258>t|g261>a|c265>t|a270>g|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g289>a,E97>N(+ - -)|g291>t,E97>N(+ - -)|g303>a|c309>t| c74>g,A25>G(- + -)|t76>g,S26>V(- - -)|c77>t,S26>V(- - -)| c87>t|g103>a,D35>N(+ + -)|t108>a,D36>E(+ - +)|t109>c,Y37>H(+ - -)|c113>g,A38>G(- + -)| c120>g,H40>Q(- + -)|t160>g,S54>A(- + -)| t168>c|t171>c|t172>g,W58>G(- - -)|g174>t,W58>G(- - -)|a184>g,S62>G(+ + -)|g188>a,G63>D(- - -)|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)| c198>a|t226>g,F76>V(+ - -)|a232>g,I78>V(+ - +)|g252>a|a253>g,N85>D(+ + -)|c258>t|g261>a|c265>t|a270>g|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g289>a,E97>N(+ - -)|g291>t,E97>N(+ - -)|g303>a|c309>t| c314>g,A105>G(- + -)|a315>c,A105>G(- + -)|a316>c,K106>R(+ + +)|a317>g,K106>R(+ + +)|a318>c,K106>R(+ + +)|g319>a,D107>I(- - -)|a320>t,D107>I(- - -)|t321>a,D107>I(- - -)|a322>g| 313 (322) 220 (229) 187 33 (42) 11 (12) 22 (30) 6 (8) 6 (7) 6 3 0 (3) 1 0 (1) 1 (2) 0 3 (4) 3 4 78 6 3 3 0 3 0 0 1 0 0 0 0 0 0 1 0 1 36 24 19 5 1 4 0 1 1 1 0 0 0 1 0 1 0 0 51 51 49 2 0 2 0 0 0 0 0 0 0 0 0 1 0 1 30 24 16 8 2 6 2 1 0 2 0 0 0 0 0 0 2 1 117 114 99 15 8 7 4 4 4 0 0 1 0 0 0 0 1 1 1 (10) 1 1 0 (9) 0 (1) 0 (8) 0 (2) 0 (1) 0 0 0 (3) 0 0 (1) 0 (1) 0 0 (1) 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ggcgtt ggattcacttttaatgaacatggc atgcagtgggtccggcaagctccagggaagggcctggagtgggtcgcaggt atcagcggtaatggtgatgtcata ggatatgcggactctgtgaagggccgagtcaccgtctccagagacaacgccaaagactctctatatttgcagatggacagtctgagagttaatgacacggccttatattattgt ggccgcatagcgaagcctggtctgggcgccttagatctc GRIAKPGLGALDL IGHV3-9 IGHD6-19 IGHJ3 ggattcacttttaatgaacatggc atgcagtgggtccggcaagctccagggaagggcctggagtgggtcgcaggt atcagcggtaatggtgatgtcata ggatatgcggactctgtgaagggccgagtcaccgtctccagagacaacgccaaagactctctatatttgcagatggacagtctgagagttaatgacacggccttatattattgt ggccgcatagcgaagcctggtctgggcgccttagatctc IGA1 30 213 20 10 11 17 9 13 8 3 9 10 JY8QFUQ01A8KQW 4893 productive Homsap IGHV3-7*01 F 909 91.24 198/217 nt NA Homsap IGHJ5*02 F 174 82.35 42/51 nt Homsap IGHD2-2*01 F 3 8 8 10 8.8.10 [1.17.38.11] CARDSPSAYDPW in-frame + ctctggattcacctttagttatcactggatgagttgggtccgccaggctccagggaaggggctggagtgggtggccctcataaggcaagatggaagtgaggaatactatgtggactctgtgaggggccgattcagcatctccagagacaacgccaagaattcagtgtacttggaaatgaacaacctgagagccgaggacacggctgtttattactgtgcgagagattccccctcagcctacgatccctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 94 [278, 279] a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c108>t,S36>Y(+ - -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)|c120>t|a163>c,N55>L(- - -)|a164>t,N55>L(- - -)|a170>g,K57>R(+ + +)|a193>g,K65>E(+ - -)|a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|c255>t|c259>g,L87>V(+ - +)|t264>c|c265>t|c268>g,Q90>E(+ + -)|g278>a,S93>N(- - -)|g303>t| a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c108>t,S36>Y(+ - -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)| c120>t|a163>c,N55>L(- - -)|a164>t,N55>L(- - -)| a170>g,K57>R(+ + +)|a193>g,K65>E(+ - -)| a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|c255>t|c259>g,L87>V(+ - +)|t264>c|c265>t|c268>g,Q90>E(+ + -)|g278>a,S93>N(- - -)|g303>t| 320 225 206 19 5 14 3 2 4 3 1 0 2 0 0 3 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 1 1 2 0 0 1 0 0 0 0 0 51 51 48 3 1 2 0 0 1 0 1 0 1 0 0 0 0 0 30 24 22 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 117 114 105 9 4 5 1 1 2 1 0 0 0 0 0 3 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagttatcactgg atgagttgggtccgccaggctccagggaaggggctggagtgggtggccctc ataaggcaagatggaagtgaggaa tactatgtggactctgtgaggggccgattcagcatctccagagacaacgccaagaattcagtgtacttggaaatgaacaacctgagagccgaggacacggctgtttattactgt gcgagagattccccctcagcctacgatccc ARDSPSAYDP IGHV3-7 IGHD2-2 IGHJ5 ggattcacctttagttatcactgg atgagttgggtccgccaggctccagggaaggggctggagtgggtggccctc ataaggcaagatggaagtgaggaa tactatgtggactctgtgaggggccgattcagcatctccagagacaacgccaagaattcagtgtacttggaaatgaacaacctgagagccgaggacacggctgtttattactgt gcgagagattccccctcagcctacgatccc IGG2 19 213 12 7 6 10 6 9 5 0 7 7 JY8QFUQ01A8KZQ 1649 productive Homsap IGHV3-7*03 F 837 87.56 190/217 nt NA Homsap IGHJ6*03 F (see comment) 183 84.31 43/51 nt Homsap IGHD6-6*01 F 3 8 8 8 8.8.8 [1.17.38.11] CTRRGFMDVW in-frame + Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) ctccggattcacttttagtgactattggatgagttgggtccgccaggctccagggaagggactggagtgggtggccaccacaaacgaggacgagactaagaaatactctgcggactctgtgaggggccgattcaccatctccagagacaacgccaagaactcactgtacttgcagatgagcagcctgagagccgacgacgcggccgtctattattgtacgagaagaggattcatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [272] t78>c|c87>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c120>t|g147>a|a164>c,N55>T(- + -)|t167>c,I56>T(- - -)|g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a174>g,Q58>E(+ + -)|t177>c|g185>a,G62>E(- - -)|a186>g,G62>E(- - -)|g188>c,S63>T(+ - +)|g190>a,E64>K(+ - -)|a200>c,Y67>S(+ - -)|t203>c,V68>A(+ - +)|a215>g,K72>R(+ + +)|t264>c|c265>t|a270>g|a275>g,N92>S(- - -)|g291>c,E97>D(+ - +)|a295>g,T99>A(- - -)|g303>c|c309>t|g313>a,A105>T(- - -)| t78>c| c87>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| c120>t|g147>a|a164>c,N55>T(- + -)| t167>c,I56>T(- - -)|g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a174>g,Q58>E(+ + -)|t177>c|g185>a,G62>E(- - -)|a186>g,G62>E(- - -)|g188>c,S63>T(+ - +)|g190>a,E64>K(+ - -)| a200>c,Y67>S(+ - -)|t203>c,V68>A(+ - +)|a215>g,K72>R(+ + +)|t264>c|c265>t|a270>g|a275>g,N92>S(- - -)|g291>c,E97>D(+ - +)|a295>g,T99>A(- - -)|g303>c|c309>t| g313>a,A105>T(- - -)|g319>a|a320>g| 318 (320) 223 (225) 195 28 (30) 10 (12) 18 7 (8) 5 (6) 4 5 2 0 0 0 4 1 0 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 21 3 1 2 1 1 1 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 1 0 0 0 0 0 0 0 30 24 15 9 1 8 2 2 0 2 0 0 0 0 2 1 0 0 117 114 103 11 5 6 4 0 2 2 1 0 0 0 2 0 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 (1) 1 (2) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcacttttagtgactattgg atgagttgggtccgccaggctccagggaagggactggagtgggtggccacc acaaacgaggacgagactaagaaa tactctgcggactctgtgaggggccgattcaccatctccagagacaacgccaagaactcactgtacttgcagatgagcagcctgagagccgacgacgcggccgtctattattgt acgagaagaggattcatggacgtc TRRGFMDV IGHV3-7 IGHD6-6 IGHJ6 ggattcacttttagtgactattgg atgagttgggtccgccaggctccagggaagggactggagtgggtggccacc acaaacgaggacgagactaagaaa tactctgcggactctgtgaggggccgattcaccatctccagagacaacgccaagaactcactgtacttgcagatgagcagcctgagagccgacgacgcggccgtctattattgt acgagaagaggattcatggacgtc IGA1 26 213 19 7 8 13 11 13 7 2 7 10 JY8QFUQ01A8LEY 5765 productive (see comment) Homsap IGHV3-48*01 F 1085 100 218/218 nt 99.54 217/218 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-2*01 F 2 8 8 10 8.8.10 [2.16.38.11] CASRLRSSGGYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 60 in the user submitted sequence), (cause frameshift) gcctctggattcaccttcagtagctatagcatgaactgggtccgccaggctccagggaagggctggagtgggtttcatacattagtagtagtagtagtaccatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagccgcctacgtagttccggtgggtactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacgatgtcgtggaactcag NA IGG1 100 100 [280] a318>c,R106>S(- - -)|g319>c|a320>g| 317 (320) 223 (226) 223 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 50 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaagggctggagtgggtttcatac attagtagtagtagtagtaccata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagccgcctacgtagttccggtgggtac ASRLRSSGGY IGHV3-48 IGHD2-2 IGHJ4 ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaagggctggagtgggtttcatac attagtagtagtagtagtaccata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagccgcctacgtagttccggtgggtac IGG1 0 212 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01A8MAI 9522 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 924 91.36 201/220 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 2 10 7 14 10.7.14 [1.17.38.11] CARRALDSSGHYIDIW in-frame + ctctggtggctccatgagcagtggtaattactgctggggctggggccgccagcccccaggaaaggggctggagtggattggaagtatgtgttatggtgggagcacctactacagcttgtcccccaagggtcgagtcaccatatccatagactcgtcgaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggctgtgtattactgtgcgagacgcgcattagacagtagtggtcattacattgacatctggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 79 95 [293, 292] c90>g,I30>M(+ + -)|a103>g,S35>G(+ + -)|g107>a,S36>N(- - -)|a113>g,Y38>C(- - -)|a124>g,I42>G(- - -)|t125>g,I42>G(- - -)|g141>a|g162>a|c168>g,I56>M(+ + -)|a170>g,Y57>C(- - -)|a175>g,S59>G(+ + -)|a203>g,N68>S(- - -)|c205>t,P69>L(- - -)|c206>t,P69>L(- - -)|t212>c,L71>P(- - -)|a220>g,S74>G(+ + -)|g238>a,V80>I(+ - +)|a244>t,T82>S(+ - +)|c249>g| c90>g,I30>M(+ + -)|a103>g,S35>G(+ + -)|g107>a,S36>N(- - -)|a113>g,Y38>C(- - -)| a124>g,I42>G(- - -)|t125>g,I42>G(- - -)|g141>a|g162>a| c168>g,I56>M(+ + -)|a170>g,Y57>C(- - -)|a175>g,S59>G(+ + -)| a203>g,N68>S(- - -)|c205>t,P69>L(- - -)|c206>t,P69>L(- - -)|t212>c,L71>P(- - -)|a220>g,S74>G(+ + -)|g238>a,V80>I(+ - +)|a244>t,T82>S(+ - +)|c249>g| a320>g| 319 (320) 227 (228) 208 19 (20) 3 (4) 16 7 (8) 4 2 1 0 0 1 0 0 3 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 0 4 2 1 0 0 0 0 0 0 0 1 0 0 51 51 47 4 2 2 1 2 0 0 0 0 0 0 0 0 0 1 30 21 18 3 0 3 2 0 0 0 0 0 0 0 0 1 0 0 117 114 106 8 1 7 2 1 2 1 0 0 1 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtggctccatgagcagtggtaattactgc tggggctggggccgccagcccccaggaaaggggctggagtggattggaagt atgtgttatggtgggagcacc tactacagcttgtcccccaagggtcgagtcaccatatccatagactcgtcgaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggctgtgtattactgt gcgagacgcgcattagacagtagtggtcattacattgacatc ARRALDSSGHYIDI IGHV4-39 IGHD3-22 IGHJ4 ggtggctccatgagcagtggtaattactgc tggggctggggccgccagcccccaggaaaggggctggagtggattggaagt atgtgttatggtgggagcacc tactacagcttgtcccccaagggtcgagtcaccatatccatagactcgtcgaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggctgtgtattactgt gcgagacgcgcattagacagtagtggtcattacattgacatc IGA2 19 216 14 5 6 9 8 10 3 0 9 7 JY8QFUQ01A8MS3 20885 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F or Homsap IGHV4-61*01 F 971 94.88 204/215 nt 94.42 203/215 nt Homsap IGHJ3*02 F 196 88 44/50 nt Homsap IGHD4-23*01 ORF 3 8 7 14 8.7.14 [1.17.38.11] CARLTTAVGGWSFDIW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 39 of V-REGION: 'C' (1 nucleotides at position 30 to 30 in the user submitted sequence), (cause frameshift); tctctggtggctccatcgatagttactacCtggacctggatccggcagcccccagggaagggactggagtggattggctatatgtattacagtaggagctccaactacaacccctccctcaagagtcgagtcaccatttcagtagacacgtccaagaagcagttctccctaaacctgagctctgtgaccgctgcggacacggccgtgtattactgtgcgagactgactacggcggtgggtggctggtcttttgatatctggggccaagggacaatggtcagcgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [289] a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g119>c,S40>T(+ - +)|g162>c|c168>g,I56>M(+ + -)|g187>a,G63>R(- - -)|a193>t,T65>S(+ - +)|a234>t|c255>g,N85>K(+ - -)|g267>a|g270>c,K90>N(+ - -)| a103>g,S35>D(- - -)|g104>a,S35>D(- - -)| g119>c,S40>T(+ - +)|g162>c| c168>g,I56>M(+ + -)|g187>a,G63>R(- - -)|a193>t,T65>S(+ - +)| a234>t|c255>g,N85>K(+ - -)|g267>a|g270>c,K90>N(+ - -)| g319>c|a320>t| 318 (320) 221 (223) 210 11 (13) 3 (5) 8 1 3 0 0 0 0 2 (3) 0 3 (4) 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 0 0 0 0 0 0 0 2 0 0 0 30 21 18 3 0 3 0 1 0 0 0 0 1 0 0 1 0 0 117 114 110 4 2 2 0 1 0 0 0 0 1 0 1 1 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcgatagttactac tggacctggatccggcagcccccagggaagggactggagtggattggctat atgtattacagtaggagctcc aactacaacccctccctcaagagtcgagtcaccatttcagtagacacgtccaagaagcagttctccctaaacctgagctctgtgaccgctgcggacacggccgtgtattactgt gcgagactgactacggcggtgggtggctggtcttttgatatc ARLTTAVGGWSFDI IGHV4-59 IGHD4-23 IGHJ3 ggtggctccatcgatagttactac tggacctggatccggcagcccccagggaagggactggagtggattggctat atgtattacagtaggagctcc aactacaacccctccctcaagagtcgagtcaccatttcagtagacacgtccaagaagcagttctccctaaacctgagctctgtgaccgctgcggacacggccgtgtattactgt gcgagactgactacggcggtgggtggctggtcttttgatatc IGA1 11 210 4 7 3 8 1 3 3 0 3 5 JY8QFUQ01A8NED 16525 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 860 88.53 193/218 nt NA Homsap IGHJ4*02 F 141 77.08 37/48 nt Homsap IGHD3-10*01 F 2 8 8 15 8.8.15 [1.17.38.11] CARDNARSYYSGVPDFW in-frame + cgtctggattcaccttcagtgaccatggcatgcactgggtccgccaggctccagggaagggtctgcagtgggtggcagttgtttggcatactggagacaataaatattatgcagagtccgtgaggggccgattcaccatctccagggacaattccaagaacacactgtatctgcaaatggacgacctgagaggcgaggacacggctatgtattattgtgcgagagataatgcccggagttattattcgggcgtgcccgacttctggggccagggaaacttggtcaccgtttcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcactctgggggcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgt NA IGG1 86 88 [295, 294] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t109>c,Y37>H(+ - -)|c141>g|g147>t|g151>c,E51>Q(+ + -)|a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|t172>c,Y58>H(+ - -)|g175>a,D59>T(- + -)|a176>c,D59>T(- + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|t189>c,S63>D(- - -)|c198>t|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|a240>g|g258>a|a274>g,N92>D(+ + -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|c287>g,A96>G(- + -)|g301>a,V101>M(+ - -)|c309>t| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t109>c,Y37>H(+ - -)| c141>g|g147>t|g151>c,E51>Q(+ + -)| a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|t172>c,Y58>H(+ - -)|g175>a,D59>T(- + -)|a176>c,D59>T(- + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|t189>c,S63>D(- - -)| c198>t|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|a240>g|g258>a|a274>g,N92>D(+ + -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|c287>g,A96>G(- + -)|g301>a,V101>M(+ - -)|c309>t| 320 226 201 25 6 19 7 6 2 3 1 0 1 0 1 3 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 1 0 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 0 0 0 0 0 0 0 1 1 1 0 30 24 16 8 0 8 2 2 0 2 1 0 1 0 0 0 0 0 117 114 103 11 4 7 4 3 2 0 0 0 0 0 0 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cgtct ggattcaccttcagtgaccatggc atgcactgggtccgccaggctccagggaagggtctgcagtgggtggcagtt gtttggcatactggagacaataaa tattatgcagagtccgtgaggggccgattcaccatctccagggacaattccaagaacacactgtatctgcaaatggacgacctgagaggcgaggacacggctatgtattattgt gcgagagataatgcccggagttattattcgggcgtgcccgacttc ARDNARSYYSGVPDF IGHV3-33 IGHD3-10 IGHJ4 ggattcaccttcagtgaccatggc atgcactgggtccgccaggctccagggaagggtctgcagtgggtggcagtt gtttggcatactggagacaataaa tattatgcagagtccgtgaggggccgattcaccatctccagggacaattccaagaacacactgtatctgcaaatggacgacctgagaggcgaggacacggctatgtattattgt gcgagagataatgcccggagttattattcgggcgtgcccgacttc IGG1 25 213 18 7 8 13 10 12 6 0 8 11 JY8QFUQ01A8SUT 14988 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 954 93.55 203/217 nt 93.09 202/217 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-2*01 F 3 8 8 11 8.8.11 [1.17.38.11] CARFGPLTRPDYW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 98 of V-REGION: 'T' (1 nucleotides at position 199 to 199 in the user submitted sequence), (cause frameshift); ctctggattcacctttaccaactatggcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtggtgatagaacataccacgcagactccgtgcagggccggttcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagccgacgaTcgcgggcgtatattactgtgcgagattcggaccacttactcgacctgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [283] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)|c164>g,A55>G(- + -)|g188>a,G63>D(- - -)|c192>a,S64>R(- - -)|t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|t246>c|g258>t|c279>t|g291>c,E97>D(+ - +)|a295>g,T99>A(- - -)|c299>g,A100>G(- + -)| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)| c164>g,A55>G(- + -)| g188>a,G63>D(- - -)|c192>a,S64>R(- - -)| t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|t246>c|g258>t|c279>t|g291>c,E97>D(+ - +)|a295>g,T99>A(- - -)|c299>g,A100>G(- + -)| a317>g,K106>R(+ + +)|g319>t|a320>t| 316 (320) 221 (225) 207 (208) 14 (17) 3 (5) 11 (12) 1 (2) 2 1 2 1 1 0 (1) 0 2 3 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 22 2 0 2 0 1 0 0 0 1 0 0 0 0 0 0 117 114 106 8 3 5 1 0 1 2 1 0 0 0 1 1 1 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgatagaaca taccacgcagactccgtgcagggccggttcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagccgacgacgcgggcgtatattactgt gcgagattcggaccacttactcgacctgactac ARFGPLTRPDY IGHV3-23 IGHD2-2 IGHJ4 ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgatagaaca taccacgcagactccgtgcagggccggttcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagccgacgacgcgggcgtatattactgt gcgagattcggaccacttactcgacctgactac IGG1 14 213 6 8 3 10 3 4 3 0 6 5 JY8QFUQ01A8VFH 12043 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 1004 95.87 209/218 nt 94.95 207/218 nt Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD2-15*01 F 2 8 8 17 8.8.17 [1.17.38.11] CARPGGIAYCSGGSCYDPW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 47 of V-REGION: 'G' (1 nucleotides at position 56 to 56 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 62 of V-REGION: 'G' (1 nucleotides at position 95 to 95 in the user submitted sequence), (cause frameshift); cctctggattcagcttcaatagttactggatgcactgggtccgccaagctccaggGgaaggggctggtgtgggtctcacatattaatattgatgGggagtaccacagactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgtgcaagaccaggggggattgcctattgtagtggtggtagctgctacgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagctccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 89 [302] c86>g,T29>S(+ - +)|g104>a,S35>N(- - -)|c108>t|g164>a,R55>H(- - +)|g173>t,S58>I(- - -)|g191>c,S64>T(+ - +)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|g258>a| c86>g,T29>S(+ - +)|g104>a,S35>N(- - -)|c108>t| g164>a,R55>H(- - +)| g173>t,S58>I(- - -)|g191>c,S64>T(+ - +)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|g258>a| g319>c|a320>c| 318 (320) 224 (226) 215 9 (11) 2 (4) 7 1 4 1 0 0 (1) 0 0 0 1 (2) 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 0 0 0 0 0 0 1 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 0 0 1 0 1 0 117 114 111 3 1 2 1 2 0 0 0 0 0 0 0 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcagcttcaatagttactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacat attaatattgatgggagtaccaca gactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt gcaagaccaggggggattgcctattgtagtggtggtagctgctacgacccc ARPGGIAYCSGGSCYDP IGHV3-74 IGHD2-15 IGHJ5 ggattcagcttcaatagttactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacat attaatattgatgggagtaccaca gactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt gcaagaccaggggggattgcctattgtagtggtggtagctgctacgacccc IGA2 9 213 6 3 5 8 1 1 1 1 3 4 JY8QFUQ01A8WCU 11607 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 1085 100 218/218 nt NA Homsap IGHJ5*02 F 255 100 51/51 nt Homsap IGHD3-9*01 F 3 8 8 15 8.8.15 [1.17.38.11] CAKDRITIFSYNWFDPW in-frame - cctctggattcacctttagcagctatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtctcagctattagtggtagtggtggtagcacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaaagatcgtattacgatattttcctacaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaac NA IGG1 100 100 [295] 320 226 226 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagcagctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagct attagtggtagtggtggtagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaagatcgtattacgatattttcctacaactggttcgacccc AKDRITIFSYNWFDP IGHV3-23 IGHD3-9 IGHJ5 ggattcacctttagcagctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagct attagtggtagtggtggtagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaagatcgtattacgatattttcctacaactggttcgacccc IGG1 0 213 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01A8XXA 1161 productive Homsap IGHV3-11*01 F 938 93.87 199/212 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 147 76.47 39/51 nt Homsap IGHD6-19*01 F 1 7 8 13 7.8.13 [X.17.38.11] CARDLQLGDDSGFSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) gattcaccctcagtgactactacatgacttggatccgccaggctccagggaaggggctggagtgggtttcgtacattagtagtctcactagttccatatattacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcactatatctgcaaatggacagcctgagagccgaggacacggccgtgtattactgtgcgagagatcttcagctgggggatgatagtgggttttcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [282] t88>c,F30>L(+ - -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|a162>g|a175>c,S59>L(- - -)|g176>t,S59>L(- - -)|t177>c,S59>L(- - -)|g184>a,G62>T(+ - -)|g185>c,G62>T(+ - -)|a190>t,T64>S(+ - +)|c198>t|g261>a|a274>g,N92>D(+ + -)| t88>c,F30>L(+ - -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|a162>g| a175>c,S59>L(- - -)|g176>t,S59>L(- - -)|t177>c,S59>L(- - -)|g184>a,G62>T(+ - -)|g185>c,G62>T(+ - -)|a190>t,T64>S(+ - +)| c198>t|g261>a|a274>g,N92>D(+ + -)| 320 220 207 13 3 10 2 2 2 2 1 0 1 0 2 0 1 0 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 22 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 51 51 48 3 1 2 1 0 1 0 0 0 0 0 1 0 0 0 30 24 18 6 0 6 0 1 0 1 1 0 1 0 1 0 1 0 117 114 111 3 2 1 1 1 1 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gattcaccctcagtgactactac atgacttggatccgccaggctccagggaaggggctggagtgggtttcgtac attagtagtctcactagttccata tattacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcactatatctgcaaatggacagcctgagagccgaggacacggccgtgtattactgt gcgagagatcttcagctgggggatgatagtgggttttcc ARDLQLGDDSGFS IGHV3-11 IGHD6-19 IGHJ5 gattcaccctcagtgactactac atgacttggatccgccaggctccagggaaggggctggagtgggtttcgtac attagtagtctcactagttccata tattacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcactatatctgcaaatggacagcctgagagccgaggacacggccgtgtattactgt gcgagagatcttcagctgggggatgatagtgggttttcc IGA1 13 212 8 5 4 7 4 6 3 0 3 7 JY8QFUQ01A8Y0H 7375 productive Homsap IGHV3-7*01 F 945 93.09 202/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-2*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSAYFSTGYW in-frame - ctctagattcacctttaggacatattggatgagttgggtccgccaagctccagggaaggggctggagtgggtggccaacataaaggaagatggaagtgagatacactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtttctccaaatgaacagcttgagagtcgatgacacggctgtgtattactgtgcgatcagtagtgcctacttctccacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [281, 280] g79>a,G27>R(- - -)|t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|c120>t|g132>a|c172>g,Q58>E(+ + -)|a194>t,K65>I(- + -)|t196>c,Y66>H(+ - -)|t256>a,S86>T(+ - +)|a263>t,Y88>F(- + -)|g267>c|c280>t|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)| g79>a,G27>R(- - -)|t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)| c120>t|g132>a| c172>g,Q58>E(+ + -)|a194>t,K65>I(- + -)| t196>c,Y66>H(+ - -)|t256>a,S86>T(+ - +)|a263>t,Y88>F(- + -)|g267>c|c280>t|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)| g317>t,R106>I(- + -)|a318>c,R106>I(- + -)|g319>a|a320>g| 316 (320) 221 (225) 206 15 (19) 4 (6) 11 (13) 0 (1) 2 (3) 3 1 0 (1) 1 2 1 2 1 1 (2) 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 0 0 0 1 0 0 1 0 0 1 51 51 49 2 2 0 0 1 1 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 117 114 107 7 2 5 0 0 2 1 0 0 1 1 1 0 1 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct agattcacctttaggacatattgg atgagttgggtccgccaagctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagata cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtttctccaaatgaacagcttgagagtcgatgacacggctgtgtattactgt gcgatcagtagtgcctacttctccacgggctac AISSAYFSTGY IGHV3-7 IGHD2-2 IGHJ4 agattcacctttaggacatattgg atgagttgggtccgccaagctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagata cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtttctccaaatgaacagcttgagagtcgatgacacggctgtgtattactgt gcgatcagtagtgcctacttctccacgggctac IGA2 15 213 6 9 5 10 1 5 4 0 5 6 JY8QFUQ01A8Y8J 15655 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 846 88.02 191/217 nt NA Homsap IGHJ3*02 F 151 78 39/50 nt Homsap IGHD2-8*02 F 2 8 8 24 8.8.24 [1.17.38.11] CVRAAGYCPGGDDCYKIGTHDSLDIW in-frame - gtctggattcaccttcagtacctatggcacacattgggtccgccaggctccaggcaaggggctggactgggtggcagttagttggcatgatggaagtcaggaatattatgcagactccgtgaggggccgattcactgtctccagagacaattccaagaacacggcatatctgcatatgaatgtcctgagaggcgaagacacggctgtctactactgtgtgagagcggcggggtactgtcctggtggtgatgactgctacaagatcggaactcatgactcccttgatatctggggccgaggggcagttgtctccgtctcctctgcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [320] g107>c,S36>T(+ - +)|t116>c,M39>T(- - -)|g117>a,M39>T(- - -)|c120>t|g153>c,E51>D(+ - +)|t167>g,I56>S(- - -)|a168>t,I56>S(- - -)|t172>c,Y58>H(+ - -)|a190>c,N64>Q(+ - +)|t192>g,N64>Q(+ - +)|a193>g,K65>E(+ - -)|c198>t|a215>g,K72>R(+ + +)|c231>t|a232>g,I78>V(+ - +)|c259>g,L87>A(+ - +)|t260>c,L87>A(+ - +)|g261>a,L87>A(+ - +)|a270>t,Q90>H(- + -)|c276>t|a277>g,S93>V(- - -)|g278>t,S93>V(- - -)|c287>g,A96>G(- + -)|g291>a|g303>c|t306>c|c314>t,A105>V(+ - +)| g107>c,S36>T(+ - +)| t116>c,M39>T(- - -)|g117>a,M39>T(- - -)|c120>t|g153>c,E51>D(+ - +)| t167>g,I56>S(- - -)|a168>t,I56>S(- - -)|t172>c,Y58>H(+ - -)|a190>c,N64>Q(+ - +)|t192>g,N64>Q(+ - +)|a193>g,K65>E(+ - -)| c198>t|a215>g,K72>R(+ + +)|c231>t|a232>g,I78>V(+ - +)|c259>g,L87>A(+ - +)|t260>c,L87>A(+ - +)|g261>a,L87>A(+ - +)|a270>t,Q90>H(- + -)|c276>t|a277>g,S93>V(- - -)|g278>t,S93>V(- - -)|c287>g,A96>G(- + -)|g291>a|g303>c|t306>c| c314>t,A105>V(+ - +)|a320>c| 319 (320) 224 (225) 197 27 (28) 7 (8) 20 4 3 5 4 1 (2) 0 2 0 3 2 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 47 4 1 3 0 1 1 1 0 0 0 0 1 0 0 0 30 24 18 6 0 6 1 0 0 1 1 0 1 0 0 0 0 2 117 114 99 15 6 9 3 2 3 2 0 0 1 0 1 2 1 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcaccttcagtacctatggc acacattgggtccgccaggctccaggcaaggggctggactgggtggcagtt agttggcatgatggaagtcaggaa tattatgcagactccgtgaggggccgattcactgtctccagagacaattccaagaacacggcatatctgcatatgaatgtcctgagaggcgaagacacggctgtctactactgt gtgagagcggcggggtactgtcctggtggtgatgactgctacaagatcggaactcatgactcccttgatatc VRAAGYCPGGDDCYKIGTHDSLDI IGHV3-33 IGHD2-8 IGHJ3 ggattcaccttcagtacctatggc acacattgggtccgccaggctccaggcaaggggctggactgggtggcagtt agttggcatgatggaagtcaggaa tattatgcagactccgtgaggggccgattcactgtctccagagacaattccaagaacacggcatatctgcatatgaatgtcctgagaggcgaagacacggctgtctactactgt gtgagagcggcggggtactgtcctggtggtgatgactgctacaagatcggaactcatgactcccttgatatc IGA1 26 213 15 11 7 13 8 13 7 0 12 7 JY8QFUQ01A91GC 13582 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 1046 97.29 215/221 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD4-11*01 ORF 2 10 7 11 10.7.11 [1.17.38.11] CARLDYTRYFDYW in-frame + tctctggtggctccatcagcagtagtagttactactggggctggatccgccagcccccagggaaggggctggagtggattgggagtatccaatatagtgggagcacctattacaatccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccagttctccctgaagctgacctctgtggccgccgcagacacggctgtgtattactgtgcgagactcgactacactaggtactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [285] t169>c,Y57>Q(- - -)|t171>a,Y57>Q(- - -)|c198>t|c204>t|g275>c,S92>T(+ - +)|a283>g,T95>A(- - -)| t169>c,Y57>Q(- - -)|t171>a,Y57>Q(- - -)| c198>t|c204>t|g275>c,S92>T(+ - +)|a283>g,T95>A(- - -)| a320>t| 319 (320) 228 (229) 222 6 (7) 2 (3) 4 1 0 2 1 0 0 0 (1) 1 1 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 19 2 0 2 0 0 0 1 0 0 0 1 0 0 0 0 117 114 110 4 2 2 1 0 2 0 0 0 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcagtagtagttactac tggggctggatccgccagcccccagggaaggggctggagtggattgggagt atccaatatagtgggagcacc tattacaatccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccagttctccctgaagctgacctctgtggccgccgcagacacggctgtgtattactgt gcgagactcgactacactaggtactttgactac ARLDYTRYFDY IGHV4-39 IGHD4-11 IGHJ4 ggtggctccatcagcagtagtagttactac tggggctggatccgccagcccccagggaaggggctggagtggattgggagt atccaatatagtgggagcacc tattacaatccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccagttctccctgaagctgacctctgtggccgccgcagacacggctgtgtattactgt gcgagactcgactacactaggtactttgactac IGA1 6 216 4 2 2 3 2 3 2 0 2 2 JY8QFUQ01A91H2 734 productive Homsap IGHV3-7*01 F 945 93.09 202/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-2*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSAYFSTGYW in-frame + ctctagattcacctttaggacatattggatgagttgggtccgccaagctccagggaaggggctggagtgggtggccaacataaaggaagatggaagtgagatacactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtttctccaaatgaacagcttgagagtcgatgacacggctgtgtattactgtgcgatcagtagtgcctacttctccacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [281] g79>a,G27>R(- - -)|t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|c120>t|g132>a|c172>g,Q58>E(+ + -)|a194>t,K65>I(- + -)|t196>c,Y66>H(+ - -)|t256>a,S86>T(+ - +)|a263>t,Y88>F(- + -)|g267>c|c280>t|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)| g79>a,G27>R(- - -)|t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)| c120>t|g132>a| c172>g,Q58>E(+ + -)|a194>t,K65>I(- + -)| t196>c,Y66>H(+ - -)|t256>a,S86>T(+ - +)|a263>t,Y88>F(- + -)|g267>c|c280>t|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)| g317>t,R106>I(- + -)|a318>c,R106>I(- + -)|g319>a|a320>g| 316 (320) 221 (225) 206 15 (19) 4 (6) 11 (13) 0 (1) 2 (3) 3 1 0 (1) 1 2 1 2 1 1 (2) 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 0 0 0 1 0 0 1 0 0 1 51 51 49 2 2 0 0 1 1 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 117 114 107 7 2 5 0 0 2 1 0 0 1 1 1 0 1 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct agattcacctttaggacatattgg atgagttgggtccgccaagctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagata cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtttctccaaatgaacagcttgagagtcgatgacacggctgtgtattactgt gcgatcagtagtgcctacttctccacgggctac AISSAYFSTGY IGHV3-7 IGHD2-2 IGHJ4 agattcacctttaggacatattgg atgagttgggtccgccaagctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagata cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtttctccaaatgaacagcttgagagtcgatgacacggctgtgtattactgt gcgatcagtagtgcctacttctccacgggctac IGA1 15 213 6 9 5 10 1 5 4 0 5 6 JY8QFUQ01A91ZM 11281 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 806 85.78 187/218 nt 85.32 186/218 nt Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD3-16*02 F 2 8 8 17 8.8.17 [1.17.38.11] CVKDRDFLWGTYRLQLEHW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 39 of V-REGION: 'G' (1 nucleotides at position 31 to 31 in the user submitted sequence), (cause frameshift); cctctggattcagatttagcagctatgccaGtgagctgggtccgccagactccagaaaaggggcttgaatgggtctcaggcatcaatgataacggtcgaagcataaactacgcgggctccgtgaagggccggttcaccatctccagagacaattccaagagcacgttgtatctgcagatggatagcctgagccccgaggactcgggcatatattattgtgtgaaagatcgtgatttcctttggggcacttatcgtcttcaacttgaacattggggccagggagccctggtcagcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [301] c86>g,T29>R(- - -)|c87>a,T29>R(- - -)|g133>a,A45>T(- - -)|g140>a,G47>E(- - -)|g141>a,G47>E(- - -)|g150>t|g153>a|c164>g,A55>G(- + -)|t165>c,A55>G(- + -)|t168>c|g170>a,S57>N(- - -)|g173>a,G58>D(- - -)|g176>a,S59>N(- - -)|t177>c,S59>N(- - -)|g187>c,G63>R(- - -)|t189>a,G63>R(- - -)|c194>t,T65>I(- - -)|t196>a,Y66>N(- - -)|a204>g|a206>g,D69>G(- - -)|a254>g,N85>S(- - -)|c259>t|a270>g|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|a295>t,T99>S(+ - +)|c299>g,A100>G(- + -)|g301>a,V101>I(+ - +)|c309>t|c314>t,A105>V(+ - +)| c86>g,T29>R(- - -)|c87>a,T29>R(- - -)| g133>a,A45>T(- - -)|g140>a,G47>E(- - -)|g141>a,G47>E(- - -)|g150>t|g153>a|c164>g,A55>G(- + -)|t165>c,A55>G(- + -)| t168>c|g170>a,S57>N(- - -)|g173>a,G58>D(- - -)|g176>a,S59>N(- - -)|t177>c,S59>N(- - -)|g187>c,G63>R(- - -)|t189>a,G63>R(- - -)|c194>t,T65>I(- - -)| t196>a,Y66>N(- - -)|a204>g|a206>g,D69>G(- - -)|a254>g,N85>S(- - -)|c259>t|a270>g|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|a295>t,T99>S(+ - +)|c299>g,A100>G(- + -)|g301>a,V101>I(+ - +)|c309>t| c314>t,A105>V(+ - +)| 320 226 194 32 7 25 5 8 5 3 1 1 1 2 2 3 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 1 0 0 0 1 0 0 51 51 44 7 2 5 0 4 0 1 0 0 0 0 0 1 1 0 30 24 16 8 1 7 0 3 1 2 0 0 0 1 1 0 0 0 117 114 100 14 4 10 5 1 3 0 1 0 1 1 1 1 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcagatttagcagctatgcc atgagctgggtccgccagactccagaaaaggggcttgaatgggtctcaggc atcaatgataacggtcgaagcata aactacgcgggctccgtgaagggccggttcaccatctccagagacaattccaagagcacgttgtatctgcagatggatagcctgagccccgaggactcgggcatatattattgt gtgaaagatcgtgatttcctttggggcacttatcgtcttcaacttgaacat VKDRDFLWGTYRLQLEH IGHV3-23 IGHD3-16 IGHJ4 ggattcagatttagcagctatgcc atgagctgggtccgccagactccagaaaaggggcttgaatgggtctcaggc atcaatgataacggtcgaagcata aactacgcgggctccgtgaagggccggttcaccatctccagagacaattccaagagcacgttgtatctgcagatggatagcctgagccccgaggactcgggcatatattattgt gtgaaagatcgtgatttcctttggggcacttatcgtcttcaacttgaacat IGA2 31 213 20 11 12 19 8 12 6 1 15 9 JY8QFUQ01A9228 20839 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 882 89.86 195/217 nt NA Homsap IGHJ3*01 F 178 84 42/50 nt Homsap IGHD6-19*01 F 1 8 8 16 8.8.16 [1.17.38.11] CAKEQRGSGWSRSALDVW in-frame + ctctggattcacgtttggcagccacgccatgagctgggtccgccaggctccagggaaggggctggagtacgtctcaattgttactggtagcggacgcagcacatactacgcagagtctgtgaagggccggttcaccgtctccagagacaattccaaggacaccctgtatctgcaaatggacagcctgagagccgaggacacggccgtgtattattgtgcgaaagaacaacgaggcagtggctggtcccgatcggctcttgatgtctggggccaaggggcatgggtcaccgtctcttcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggctgacggtgtcgtggaactc NA IGG2 100 100 [297] c87>g|a103>g,S35>G(+ + -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|g163>a,A55>I(+ - +)|c164>t,A55>I(+ - +)|a166>g,I56>V(+ - +)|g170>c,S57>T(+ - +)|t177>c|t186>a|g187>c,G63>R(- - -)|t189>c,G63>R(- - -)|c207>g,D69>E(+ - +)|c210>t|a232>g,I78>V(+ - +)|a253>g,N85>D(+ + -)|g258>c|a274>g,N92>D(+ + -)|a303>g|c309>t| c87>g|a103>g,S35>G(+ + -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)| g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|g163>a,A55>I(+ - +)|c164>t,A55>I(+ - +)| a166>g,I56>V(+ - +)|g170>c,S57>T(+ - +)|t177>c|t186>a|g187>c,G63>R(- - -)|t189>c,G63>R(- - -)| c207>g,D69>E(+ - +)|c210>t|a232>g,I78>V(+ - +)|a253>g,N85>D(+ + -)|g258>c|a274>g,N92>D(+ + -)|a303>g|c309>t| 320 225 203 22 7 15 6 2 3 4 0 0 0 1 4 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 0 2 0 0 0 0 0 1 0 0 51 51 47 4 0 4 0 2 1 0 0 0 0 0 1 0 0 0 30 24 18 6 2 4 1 0 0 2 0 0 0 1 2 0 0 0 117 114 106 8 4 4 4 0 2 0 0 0 0 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgtttggcagccacgcc atgagctgggtccgccaggctccagggaaggggctggagtacgtctcaatt gttactggtagcggacgcagcaca tactacgcagagtctgtgaagggccggttcaccgtctccagagacaattccaaggacaccctgtatctgcaaatggacagcctgagagccgaggacacggccgtgtattattgt gcgaaagaacaacgaggcagtggctggtcccgatcggctcttgatgtc AKEQRGSGWSRSALDV IGHV3-23 IGHD6-19 IGHJ3 ggattcacgtttggcagccacgcc atgagctgggtccgccaggctccagggaaggggctggagtacgtctcaatt gttactggtagcggacgcagcaca tactacgcagagtctgtgaagggccggttcaccgtctccagagacaattccaaggacaccctgtatctgcaaatggacagcctgagagccgaggacacggccgtgtattattgt gcgaaagaacaacgaggcagtggctggtcccgatcggctcttgatgtc IGG2 22 213 15 7 5 11 10 11 4 3 8 7 JY8QFUQ01A928N 6391 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 967 94.44 204/216 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-3*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARDCDSGECNFHYW in-frame + tctggattcagcttcagtacctataacatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccataagtagtggtagtacttacatatatcacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaagacactttacctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagattgtgacagtggtgaatgtaactttcactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 100 100 [287] c86>g,T29>S(+ - +)|g107>c,S36>T(+ - +)|g113>a,S38>N(- - -)|t168>a|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|c198>t|t199>c,Y67>H(+ - -)|c255>g,N85>K(+ - -)|t256>a,S86>T(+ - +)|g261>t|t264>c| c86>g,T29>S(+ - +)|g107>c,S36>T(+ - +)|g113>a,S38>N(- - -)| t168>a|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)| c198>t|t199>c,Y67>H(+ - -)|c255>g,N85>K(+ - -)|t256>a,S86>T(+ - +)|g261>t|t264>c| 320 224 212 12 4 8 1 1 1 2 0 0 0 2 2 2 1 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 1 0 0 0 0 0 0 1 1 0 0 0 117 114 108 6 3 3 0 0 1 2 0 0 0 1 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcagcttcagtacctataac atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc ataagtagtggtagtacttacata tatcacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaagacactttacctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagattgtgacagtggtgaatgtaactttcactac ARDCDSGECNFHY IGHV3-21 IGHD3-3 IGHJ4 ggattcagcttcagtacctataac atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc ataagtagtggtagtacttacata tatcacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaagacactttacctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagattgtgacagtggtgaatgtaactttcactac IGG1 12 213 5 7 2 7 3 5 3 1 3 5 JY8QFUQ01A94FS 9063 productive Homsap IGHV3-9*02 F 886 90.28 195/216 nt NA Homsap IGHJ6*02 F 172 78.57 44/56 nt Homsap IGHD1-14*01 ORF 2 8 8 11 8.8.11 [1.17.38.11] CGKDMRPGGMDVW in-frame + tccggattcacctctggaaagtatgccatgcactgggtccggcaagctccagggaaggacctggagtgggtctcaggcttgggtttggataatggtaggatagactacgcggactctgtgaagggccgattcaccatctccaaagacaacgccaagaattccctgtatctgcaaatgaacagcctgagagttgaggacacggccatgtattactgtggaaaagatatgcgaccgggcggtatggacgtctggggccaggggatcacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaaggatgggaacgtggtcgtcgcatgcctggtcccagggcttcttccccc NA IGA2 71 89 [280, 282] t78>c|a104>g,D35>G(- - -)|t105>a,D35>G(- - -)|g106>a,D36>K(+ - -)|t108>g,D36>K(+ - -)|g146>a,G49>D(- - -)|t165>c|a166>t,I56>L(+ + +)|t168>g,I56>L(+ + +)|a169>g,S57>G(+ + -)|g173>t,W58>L(+ - -)|a175>g,N59>D(+ + -)|g185>a,S62>N(- - -)|c192>g,S64>R(- - -)|g197>a,G66>D(- - -)|t201>c|g239>a,R80>K(+ + +)|c255>t|t279>c|c287>t,A96>V(+ - +)|t301>a,L101>M(+ + -)|c314>g,A105>G(- + -)| t78>c| a104>g,D35>G(- - -)|t105>a,D35>G(- - -)|g106>a,D36>K(+ - -)|t108>g,D36>K(+ - -)| g146>a,G49>D(- - -)|t165>c| a166>t,I56>L(+ + +)|t168>g,I56>L(+ + +)|a169>g,S57>G(+ + -)|g173>t,W58>L(+ - -)|a175>g,N59>D(+ + -)|g185>a,S62>N(- - -)|c192>g,S64>R(- - -)| g197>a,G66>D(- - -)|t201>c|g239>a,R80>K(+ + +)|c255>t|t279>c|c287>t,A96>V(+ - +)|t301>a,L101>M(+ + -)| c314>g,A105>G(- + -)| 322 226 204 22 5 17 3 5 2 4 0 0 1 2 0 2 1 2 78 3 2 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 0 0 0 0 0 1 0 0 0 1 51 51 49 2 1 1 0 1 0 1 0 0 0 0 0 0 0 0 30 24 17 7 0 7 2 1 0 0 0 0 1 0 0 1 1 1 117 114 107 7 3 4 0 2 2 2 0 0 0 1 0 0 0 0 10 10 9 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| tcc ggattcacctctggaaagtatgcc atgcactgggtccggcaagctccagggaaggacctggagtgggtctcaggc ttgggtttggataatggtaggata gactacgcggactctgtgaagggccgattcaccatctccaaagacaacgccaagaattccctgtatctgcaaatgaacagcctgagagttgaggacacggccatgtattactgt ggaaaagatatgcgaccgggcggtatggacgtc GKDMRPGGMDV IGHV3-9 IGHD1-14 IGHJ6 ggattcacctctggaaagtatgcc atgcactgggtccggcaagctccagggaaggacctggagtgggtctcaggc ttgggtttggataatggtaggata gactacgcggactctgtgaagggccgattcaccatctccaaagacaacgccaagaattccctgtatctgcaaatgaacagcctgagagttgaggacacggccatgtattactgt ggaaaagatatgcgaccgggcggtatggacgtc IGA2 20 213 13 7 7 9 6 11 4 0 5 11 JY8QFUQ01A96A4 20609 productive Homsap IGHV1-3*01 F 876 90.19 193/214 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD6-25*01 F 1 8 8 13 8.8.13 [0.17.38.11] CARDGYRSGNGADYW in-frame - tggatacagcttcactaactacaatatccattgggtgcgccaggcccccggacaagggcttgagtgggtgggatggatcaacgctggcaatggcaatacaagatattcacagaaattgcagggcagagtcaccatttccagggacacatccgcgagcattgccaacatggagttgagcagcctgagatatgaagacacggctgtatattattgtgcgagagacggctataggagtggcaacggggctgactattggggccagggaaccctggtcaccgtctccccagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|t111>c|g112>a,A38>N(- - -)|c113>a,A38>N(- - -)|g117>c,M39>I(+ + -)|a145>g,R49>G(- - -)|a157>g,M53>V(+ - -)|t189>c|c192>t|a197>g,K66>R(+ + +)|g210>a|c213>g,F71>L(+ - -)|a235>t,T79>S(+ - +)|c257>t,T86>I(- - -)|a258>t,T86>I(- - -)|t262>a,Y88>N(- - -)|c271>t|c287>a,S96>Y(+ - -)|g303>a|c309>t| c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|t111>c|g112>a,A38>N(- - -)|c113>a,A38>N(- - -)| g117>c,M39>I(+ + -)|a145>g,R49>G(- - -)|a157>g,M53>V(+ - -)| t189>c|c192>t| a197>g,K66>R(+ + +)|g210>a|c213>g,F71>L(+ - -)|a235>t,T79>S(+ - +)|c257>t,T86>I(- - -)|a258>t,T86>I(- - -)|t262>a,Y88>N(- - -)|c271>t|c287>a,S96>Y(+ - -)|g303>a|c309>t| 320 222 201 21 7 14 3 4 4 2 0 2 2 1 1 2 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 2 0 1 0 1 0 0 0 1 0 0 51 51 48 3 0 3 2 0 0 0 0 0 0 0 1 0 0 0 30 24 22 2 2 0 0 0 1 1 0 0 0 0 0 0 0 0 117 114 103 11 4 7 1 2 3 0 0 1 2 1 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|ta,262-263(FR3)|aa,290-291(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,11-12(FR1)|tt,62-63(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,120-121(FR2)|tt,149-150(FR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,201-202(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacagcttcactaactacaat atccattgggtgcgccaggcccccggacaagggcttgagtgggtgggatgg atcaacgctggcaatggcaataca agatattcacagaaattgcagggcagagtcaccatttccagggacacatccgcgagcattgccaacatggagttgagcagcctgagatatgaagacacggctgtatattattgt gcgagagacggctataggagtggcaacggggctgactat ARDGYRSGNGADY IGHV1-3 IGHD6-25 IGHJ4 ggatacagcttcactaactacaat atccattgggtgcgccaggcccccggacaagggcttgagtgggtgggatgg atcaacgctggcaatggcaataca agatattcacagaaattgcagggcagagtcaccatttccagggacacatccgcgagcattgccaacatggagttgagcagcctgagatatgaagacacggctgtatattattgt gcgagagacggctataggagtggcaacggggctgactat IGA1 21 213 13 8 8 13 5 8 4 3 10 4 JY8QFUQ01A96T1 8923 productive (see comment) Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 892 89.95 197/219 nt 89.5 196/219 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD6-19*01 F 1 8 8 13 8.8.13 [2.17.38.11] CARDPSGSGWAFESW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'AT' (2 nucleotides at position 10 to 11 in the user submitted sequence), (cause frameshift); gcgtctggaATttccctttcagtagagatgccatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatggtatggtatgatggaagtaatacacaccatgcagattccgtgaagggccgattcatcatttccagagacaattccaagaataaagtgtatctgcaaatgaacagtctgagagacgaggacacggctgtctattattgtgcgagagatccgagcggcagtggctgggcctttgagtcctggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [291, 290] a85>c,T29>P(+ + -)|c87>t,T29>P(+ + -)|c108>a,S36>R(- - -)|t109>g,Y37>D(- - -)|g113>c,G38>A(- + -)|g163>a,V55>M(+ - -)|t165>g,V55>M(+ - -)|a166>g,I56>V(+ - +)|a194>c,K65>T(- - -)|t196>c,Y66>H(+ - -)|t199>c,Y67>H(+ - -)|c207>t|c230>t,T77>I(- - -)|c234>t|c255>t|c257>a,T86>K(- - -)|g258>a,T86>K(- - -)|c259>g,L87>V(+ - +)|c279>t|c287>a,A96>D(- - -)|g303>c|c309>t| a85>c,T29>P(+ + -)|c87>t,T29>P(+ + -)|c108>a,S36>R(- - -)|t109>g,Y37>D(- - -)|g113>c,G38>A(- + -)| g163>a,V55>M(+ - -)|t165>g,V55>M(+ - -)| a166>g,I56>V(+ - +)|a194>c,K65>T(- - -)| t196>c,Y66>H(+ - -)|t199>c,Y67>H(+ - -)|c207>t|c230>t,T77>I(- - -)|c234>t|c255>t|c257>a,T86>K(- - -)|g258>a,T86>K(- - -)|c259>g,L87>V(+ - +)|c279>t|c287>a,A96>D(- - -)|g303>c|c309>t| 320 227 205 22 6 16 1 2 7 2 2 3 0 0 2 1 0 2 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 0 1 0 1 1 0 0 1 0 0 1 51 51 49 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 30 24 22 2 0 2 1 0 0 0 1 0 0 0 0 0 0 0 117 114 101 13 6 7 0 1 6 2 0 2 0 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcgtct ggattccctttcagtagagatgcc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatg gtatggtatgatggaagtaataca caccatgcagattccgtgaagggccgattcatcatttccagagacaattccaagaataaagtgtatctgcaaatgaacagtctgagagacgaggacacggctgtctattattgt gcgagagatccgagcggcagtggctgggcctttgagtcc ARDPSGSGWAFES IGHV3-33 IGHD6-19 IGHJ4 ggattccctttcagtagagatgcc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatg gtatggtatgatggaagtaataca caccatgcagattccgtgaagggccgattcatcatttccagagacaattccaagaataaagtgtatctgcaaatgaacagtctgagagacgaggacacggctgtctattattgt gcgagagatccgagcggcagtggctgggcctttgagtcc IGA2 22 213 12 10 9 15 3 7 6 0 9 7 JY8QFUQ01A98LD 14680 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 864 88.94 193/217 nt NA Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F (see comment) 186 87.5 42/48 nt Homsap IGHD6-6*01 F 2 8 8 15 8.8.15 [1.17.38.11] CAKGDSPIASRLYIDSW in-frame + Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) ctctggattcatttttagcaattatgccatgaactgggtccgccaggctccagggaaggggccggagtgggtctcagcttttagtggtggtggcactaagacctactacgcagactccgtgaagggccggttcttcatctccagagacaattccaagaacactctacatctgcatatgagcagcctgagggccgaggacacggccacatattactgtgcgaaaggggactcgcctatagcgtctcgtctatatattgactcctggggccaggggaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [294] c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g119>a,S40>N(- - -)|t149>c,L50>P(- - -)|a166>t,I56>F(+ - -)|a175>g,S59>G(+ + -)|t186>c|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|g191>a,S64>K(- - -)|c192>g,S64>K(- - -)|a195>c|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|g258>t|g261>a|t262>c,Y88>H(+ - -)|a270>t,Q90>H(- + -)|a275>g,N92>S(- - -)|a285>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g119>a,S40>N(- - -)|t149>c,L50>P(- - -)| a166>t,I56>F(+ - -)|a175>g,S59>G(+ + -)|t186>c|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|g191>a,S64>K(- - -)|c192>g,S64>K(- - -)|a195>c| a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|g258>t|g261>a|t262>c,Y88>H(+ - -)|a270>t,Q90>H(- + -)|a275>g,N92>S(- - -)|a285>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)| a320>g| 319 (320) 224 (225) 200 24 (25) 5 (6) 19 3 (4) 6 4 4 1 0 3 0 1 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 3 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 30 24 16 8 2 6 1 2 0 1 1 0 1 0 1 1 0 0 117 114 104 10 3 7 2 2 1 2 0 0 2 0 0 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatttttagcaattatgcc atgaactgggtccgccaggctccagggaaggggccggagtgggtctcagct tttagtggtggtggcactaagacc tactacgcagactccgtgaagggccggttcttcatctccagagacaattccaagaacactctacatctgcatatgagcagcctgagggccgaggacacggccacatattactgt gcgaaaggggactcgcctatagcgtctcgtctatatattgactcc AKGDSPIASRLYIDS IGHV3-23 IGHD6-6 IGHJ4 ggattcatttttagcaattatgcc atgaactgggtccgccaggctccagggaaggggccggagtgggtctcagct tttagtggtggtggcactaagacc tactacgcagactccgtgaagggccggttcttcatctccagagacaattccaagaacactctacatctgcatatgagcagcctgagggccgaggacacggccacatattactgt gcgaaaggggactcgcctatagcgtctcgtctatatattgactcc IGG2 24 213 17 7 10 13 7 11 3 2 9 10 JY8QFUQ01A98NE 8099 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 858 89.25 191/214 nt 88.79 190/214 nt Homsap IGHJ3*02 F 232 96 48/50 nt Homsap IGHD2-15*01 F 2 8 7 20 8.7.20 [1.16.38.11] CARDPRDYCSGGYCFVGAFDIW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 47 of V-REGION: 1 nucleotides (from position 56 in the user submitted sequence), (cause frameshift) tctctggtggctccatgaggaattattactggagctggatccggcagtccccaggaagggactggagttgatagggactgtctattacactgggcgcacggagtacaacccctccctcaagagtcgactcaccttatcactagacacgtccaagaaccagttctccctaaagctgggctctgtgaccgctgcggactcggccatttattactgtgcgagagacccccgagactattgtagtggtggttactgttttgttggtgcttttgatatctggggccaagggacaaaggtcaccgtctcttcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 100 100 [306] c90>g,I30>M(+ + -)|t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t|c133>t,P45>S(+ - -)|g155>t,W52>L(+ - -)|t159>a|t163>a,Y55>T(+ - -)|a164>c,Y55>T(+ - -)|a166>g,I56>V(+ - +)|g176>c,S59>T(+ - +)|a190>c,S64>R(- - -)|c195>g|a196>g,N66>E(+ - -)|c198>g,N66>E(+ - -)|g226>c,V76>L(+ - +)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|g267>a|a274>g,S92>G(+ + -)|a295>t,T99>S(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| c90>g,I30>M(+ + -)|t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t| c133>t,P45>S(+ - -)|g155>t,W52>L(+ - -)|t159>a|t163>a,Y55>T(+ - -)|a164>c,Y55>T(+ - -)| a166>g,I56>V(+ - +)|g176>c,S59>T(+ - +)|a190>c,S64>R(- - -)|c195>g| a196>g,N66>E(+ - -)|c198>g,N66>E(+ - -)|g226>c,V76>L(+ - +)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|g267>a|a274>g,S92>G(+ + -)|a295>t,T99>S(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| 320 222 199 23 4 19 3 3 2 0 2 0 2 2 3 3 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 1 0 0 0 0 0 0 1 0 1 51 50 45 5 1 4 0 0 1 0 1 0 0 2 0 0 1 0 30 21 17 4 1 3 1 0 0 0 1 0 0 0 1 1 0 0 117 114 104 10 1 9 2 2 0 0 0 0 2 0 2 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatgaggaattattac tggagctggatccggcagtccccaggaagggactggagttgatagggact gtctattacactgggcgcacg gagtacaacccctccctcaagagtcgactcaccttatcactagacacgtccaagaaccagttctccctaaagctgggctctgtgaccgctgcggactcggccatttattactgt gcgagagacccccgagactattgtagtggtggttactgttttgttggtgcttttgatatc ARDPRDYCSGGYCFVGAFDI IGHV4-59 IGHD2-15 IGHJ3 ggtggctccatgaggaattattac tggagctggatccggcagtccccaggaagggactggagttgatagggact gtctattacactgggcgcacg gagtacaacccctccctcaagagtcgactcaccttatcactagacacgtccaagaaccagttctccctaaagctgggctctgtgaccgctgcggactcggccatttattactgt gcgagagacccccgagactattgtagtggtggttactgttttgttggtgcttttgatatc IGG1 23 209 8 15 5 13 3 10 2 2 13 6 JY8QFUQ01A9C87 13940 productive Homsap IGHV4-39*02 F 952 91.89 204/222 nt NA Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD1-26*01 F 3 10 7 13 10.7.13 [2.17.38.11] CARRYSWNYERFDPW in-frame - gtctctggtgactccatcagtactaataattactactgggcctggatccgccagcccccagggagggggctggagtggattgggaatatctattatagcgggaccacctactacaatccgtccctcaagagtcgagtcaccatgtccgtagacacgtccaagaaccacttctccctgaggttgagttctgtgaccgccgcagacacggctctctattactgcgcgcggcgatatagttggaactacgaaaggttcgacccctggggccagggaaccctggtcatcgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [293] g83>a,G28>D(- - -)|c93>t|g101>c,S34>T(+ - +)|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|g119>c,G40>A(- + -)|a143>g,K48>R(+ + +)|g164>a,S55>N(- - -)|t177>c|g191>c,S64>T(+ - +)|c204>t|a234>g,I78>M(+ + -)|a269>g,K90>R(+ + +)|c271>t|c276>t|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t312>c| g83>a,G28>D(- - -)|c93>t|g101>c,S34>T(+ - +)|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)| g119>c,G40>A(- + -)|a143>g,K48>R(+ + +)|g164>a,S55>N(- - -)| t177>c|g191>c,S64>T(+ - +)| c204>t|a234>g,I78>M(+ + -)|a269>g,K90>R(+ + +)|c271>t|c276>t|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t312>c| a316>c|a318>g|g319>c|a320>g| 315 (320) 225 (230) 207 (208) 18 (22) 6 (10) 12 3 (5) 4 4 2 0 (1) 0 0 0 5 (6) 0 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 25 5 1 4 0 3 1 0 0 0 0 0 1 0 0 0 51 51 48 3 0 3 1 1 0 0 0 0 0 0 1 0 0 0 30 21 19 2 1 1 0 0 0 1 0 0 0 0 1 0 0 0 117 114 106 8 4 4 2 0 3 1 0 0 0 0 2 0 0 0 3 (8) 3 3 (4) 0 (4) 0 (4) 0 0 (2) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct ggtgactccatcagtactaataattactac tgggcctggatccgccagcccccagggagggggctggagtggattgggaat atctattatagcgggaccacc tactacaatccgtccctcaagagtcgagtcaccatgtccgtagacacgtccaagaaccacttctccctgaggttgagttctgtgaccgccgcagacacggctctctattactgc gcgcggcgatatagttggaactacgaaaggttcgacccc ARRYSWNYERFDP IGHV4-39 IGHD1-26 IGHJ5 ggtgactccatcagtactaataattactac tgggcctggatccgccagcccccagggagggggctggagtggattgggaat atctattatagcgggaccacc tactacaatccgtccctcaagagtcgagtcaccatgtccgtagacacgtccaagaaccacttctccctgaggttgagttctgtgaccgccgcagacacggctctctattactgc gcgcggcgatatagttggaactacgaaaggttcgacccc IGG1 18 216 13 5 8 13 5 5 4 2 7 5 JY8QFUQ01A9CIV 14572 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 964 93.61 205/219 nt 92.69 203/219 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [2.17.38.11] CVRAGFGELSGFDCW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'C' (1 nucleotides at position 15 to 15 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 47 of V-REGION: 'G' (1 nucleotides at position 57 to 57 in the user submitted sequence), (cause frameshift); gcctctggattcacCctttggttattatggcatgagctgggtccgccaagctccggGggagggggctggagtgggtctctggtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [292] a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a138>g|a143>g,K48>R(+ + +)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a138>g|a143>g,K48>R(+ + +)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 226 (227) 211 15 (16) 3 (4) 12 3 3 1 1 1 (2) 0 2 0 2 1 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 51 49 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| gcctct ggattcacctttggttattatggc atgagctgggtccgccaagctccggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgagctgggtccgccaagctccggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 14 213 7 7 3 7 4 7 3 0 7 4 JY8QFUQ01A9D9E 21439 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 851 88.07 192/218 nt NA Homsap IGHJ4*02 F, or Homsap IGHJ5*02 F 109 70.21 33/47 nt Homsap IGHD5-12*01 F 3 8 8 6 8.8.6 [1.17.38.11] CVSIGHGV in-frame + cctctggattcgcctttaggagtgagtggatgaactgggtccgccaagccccagggaaggggctggagtgggtcgcacacattgacactgatgggagtatcgcagtctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaatactttgtatctgcaaatggacagtctgagagccgacgacacggctatatattactgtgtaagcattggacacggagtgggcccgggaacccaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtcccagggcttcttcccccg NA IGA2 100 100 [267] a85>g,T29>A(- - -)|c90>t|t105>g,S35>R(- - -)|c108>t|t109>g,Y37>E(- - -)|c111>g,Y37>E(- - -)|c118>a,H40>N(- - -)|t135>c|t152>a,V51>E(- + -)|t160>g,S54>A(- + -)|g164>a,R55>H(- - +)|t165>c,R55>H(- - +)|a169>g,N57>D(+ + -)|t171>c,N57>D(+ + -)|g173>c,S58>T(+ - +)|g191>t,S64>I(- - -)|a193>g,T65>A(- - -)|a196>g,S66>V(- - -)|g197>t,S66>V(- - -)|c255>t|g258>t|c259>t|a274>g,N92>D(+ + -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c314>t,A105>V(+ - +)| a85>g,T29>A(- - -)|c90>t|t105>g,S35>R(- - -)|c108>t|t109>g,Y37>E(- - -)|c111>g,Y37>E(- - -)| c118>a,H40>N(- - -)|t135>c|t152>a,V51>E(- + -)|t160>g,S54>A(- + -)|g164>a,R55>H(- - +)|t165>c,R55>H(- - +)| a169>g,N57>D(+ + -)|t171>c,N57>D(+ + -)|g173>c,S58>T(+ - +)|g191>t,S64>I(- - -)|a193>g,T65>A(- - -)| a196>g,S66>V(- - -)|g197>t,S66>V(- - -)|c255>t|g258>t|c259>t|a274>g,N92>D(+ + -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| c314>t,A105>V(+ - +)|a318>c,R106>S(- - -)|g319>a|a320>t| 317 (320) 223 (226) 196 27 (30) 6 (8) 21 (22) 5 3 (4) 5 3 0 (1) 1 0 (1) 1 2 1 3 3 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 2 4 1 0 2 0 0 0 0 0 0 1 0 2 51 51 45 6 1 5 0 1 0 2 0 1 0 1 0 0 0 1 30 24 19 5 0 5 2 0 0 1 0 0 0 0 1 0 1 0 117 114 105 9 3 6 2 2 2 0 0 0 0 0 1 0 2 0 5 (8) 5 4 1 (4) 0 (2) 1 (2) 0 0 (1) 1 0 0 (1) 0 0 (1) 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcgcctttaggagtgagtgg atgaactgggtccgccaagccccagggaaggggctggagtgggtcgcacac attgacactgatgggagtatcgca gtctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaatactttgtatctgcaaatggacagtctgagagccgacgacacggctatatattactgt gtaagcattggacacgga VSIGHG IGHV3-74 IGHD5-12 IGHJ4 ggattcgcctttaggagtgagtgg atgaactgggtccgccaagccccagggaaggggctggagtgggtcgcacac attgacactgatgggagtatcgca gtctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaatactttgtatctgcaaatggacagtctgagagccgacgacacggctatatattactgt gtaagcattggacacgga IGA2 26 213 15 11 7 14 8 12 4 2 11 9 JY8QFUQ01A9DAX 16345 productive Homsap IGHV3-7*02 F 909 91.24 198/217 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD5-12*01 F 1 8 8 9 8.8.9 [1.17.38.11] CARDQVTEDYW in-frame + ctctggattcctttttagaacctattggatgagttgggtccgccaggctccagggaaagggctggagtgggtggcccacataaaccaagatggacgtgaggcatactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgtgccagagatcaagtgactgaagactactggggccagggaaccctggtcagtgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaaactc NA IGG1 100 100 [276] a85>c,T29>L(- - -)|c86>t,T29>L(- - -)|c87>t,T29>L(- - -)|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|c120>t|a163>c,N55>H(- - -)|g171>c,K57>N(+ - -)|a187>c,S63>R(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)|c248>g,A83>G(- + -)|c255>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|g297>c|g301>a,V101>M(+ - -)|c309>t|g315>c| a85>c,T29>L(- - -)|c86>t,T29>L(- - -)|c87>t,T29>L(- - -)|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)| c120>t|a163>c,N55>H(- - -)| g171>c,K57>N(+ - -)|a187>c,S63>R(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)| c248>g,A83>G(- + -)|c255>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|g297>c|g301>a,V101>M(+ - -)|c309>t| g315>c| 318 223 203 20 6 14 1 2 6 0 4 0 0 1 4 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 0 2 0 1 0 0 1 1 0 0 0 51 51 49 2 1 1 0 0 1 0 1 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 0 0 2 0 0 0 1 0 0 0 117 114 106 8 4 4 0 2 3 0 0 0 0 0 1 2 0 0 6 6 5 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcctttttagaacctattgg atgagttgggtccgccaggctccagggaaagggctggagtgggtggcccac ataaaccaagatggacgtgaggca tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac ARDQVTEDY IGHV3-7 IGHD5-12 IGHJ4 ggattcctttttagaacctattgg atgagttgggtccgccaggctccagggaaagggctggagtgggtggcccac ataaaccaagatggacgtgaggca tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac IGG1 19 213 9 10 8 13 1 6 5 0 5 9 JY8QFUQ01A9DEW 19531 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 936 92.63 201/217 nt NA Homsap IGHJ3*01 F (see comment) 160 80 40/50 nt Homsap IGHD6-13*01 F 3 8 8 14 8.8.14 [1.17.38.11] CARDLRARLWHPMDVW in-frame + Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) ctccggattcagtttcagtacttataacatgaattgggtccgccaggctccagggaaggggctggagtgggtctcatccatgggtagtagtagtatttacatatattacgcagactcagtgaagggccgattcaccatctccagagacgacgccaagagttcactgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgtgcgagagacttaagggctcgactatggcatccgatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaagggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 94 [290, 291] t78>c|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g113>a,S38>N(- - -)|c120>t|t168>g,I56>M(+ + -)|a169>g,S57>G(+ + -)|g188>t,S63>I(- - -)|c198>t|a244>g,N82>D(+ + -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| t78>c| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g113>a,S38>N(- - -)| c120>t| t168>g,I56>M(+ + -)|a169>g,S57>G(+ + -)|g188>t,S63>I(- - -)| c198>t|a244>g,N82>D(+ + -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 225 209 16 3 13 3 3 5 1 0 0 0 0 1 1 1 1 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 1 2 0 0 0 0 0 1 1 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 0 0 1 1 117 114 108 6 1 5 2 2 2 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcagtttcagtacttataac atgaattgggtccgccaggctccagggaaggggctggagtgggtctcatcc atgggtagtagtagtatttacata tattacgcagactcagtgaagggccgattcaccatctccagagacgacgccaagagttcactgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgt gcgagagacttaagggctcgactatggcatccgatggacgtc ARDLRARLWHPMDV IGHV3-21 IGHD6-13 IGHJ3 ggattcagtttcagtacttataac atgaattgggtccgccaggctccagggaaggggctggagtgggtctcatcc atgggtagtagtagtatttacata tattacgcagactcagtgaagggccgattcaccatctccagagacgacgccaagagttcactgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgt gcgagagacttaagggctcgactatggcatccgatggacgtc IGG1 15 213 11 4 8 11 3 4 2 0 5 8 JY8QFUQ01A9DTT 4655 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 804 86.45 185/214 nt 85.51 183/214 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-15*01 F 2 8 7 10 8.7.10 [1.17.37.11] CATLRGGTFDYW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 56 of V-REGION: 'C' (1 nucleotides at position 82 to 82 in the user submitted sequence), (cause frameshift); in FR3-IMGT, from codon 68 of V-REGION: 1 nucleotides (from position 110 in the user submitted sequence), (cause frameshift) tctctggtgcctccatcaggagttattattggagttggatccggcagcccccaggaaagggactggagtggattggttataCttaattatgttggggacaccgattacaaccctccctcaagagtcgagtctccatgtcagcagccacgtccaagaaccaggtcttcctgcagctgacctctgtgaccgctgcggacaccgcctattatttctgtgcgacactgagagggggtactttcgactactggggccggggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [277] g83>c,G28>A(- + -)|t105>g,S35>R(- - -)|c111>t|c114>t|c120>t|g141>a|g162>t|c168>t|t169>a,Y57>N(- - -)|c174>t|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a196>g,N66>D(+ + -)|c198>t,N66>D(+ + -)|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|t239>c,V80>A(+ - +)|a242>c,D81>A(- - -)|t259>g,F87>V(+ - -)|c263>t,S88>F(- - -)|a268>c,K90>Q(+ - -)|g275>c,S92>T(+ - +)|g297>c|g301>t,V101>Y(- - -)|t302>a,V101>Y(- - -)|g303>t,V101>Y(- - -)|a308>t,Y103>F(- + -)| g83>c,G28>A(- + -)|t105>g,S35>R(- - -)|c111>t|c114>t| c120>t|g141>a|g162>t| c168>t|t169>a,Y57>N(- - -)|c174>t|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a196>g,N66>D(+ + -)|c198>t,N66>D(+ + -)|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|t239>c,V80>A(+ - +)|a242>c,D81>A(- - -)|t259>g,F87>V(+ - -)|c263>t,S88>F(- - -)|a268>c,K90>Q(+ - -)|g275>c,S92>T(+ - +)|g297>c|g301>t,V101>Y(- - -)|t302>a,V101>Y(- - -)|g303>t,V101>Y(- - -)|a308>t,Y103>F(- + -)| g317>c,R106>T(- - -)|g319>c|a320>t| 316 (320) 218 (222) 189 (190) 29 (32) 8 (10) 21 (22) 4 2 7 1 2 0 2 (3) 2 3 (5) 0 4 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 0 2 0 0 0 0 0 1 0 0 1 51 51 48 3 3 0 0 1 1 0 0 0 0 0 0 0 1 0 30 21 14 7 2 5 2 1 2 0 0 0 0 1 0 0 1 0 117 113 98 15 1 14 2 0 2 1 2 0 2 1 2 0 2 1 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 0 0 0 0 0 0 (1) 0 0 (2) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgcctccatcaggagttattat tggagttggatccggcagcccccaggaaagggactggagtggattggttat attaattatgttggggacacc gattacaaccctccctcaagagtcgagtctccatgtcagcagccacgtccaagaaccaggtcttcctgcagctgacctctgtgaccgctgcggacaccgcctattatttctgt gcgacactgagagggggtactttcgactac ATLRGGTFDY IGHV4-59 IGHD2-15 IGHJ4 ggtgcctccatcaggagttattat tggagttggatccggcagcccccaggaaagggactggagtggattggttat attaattatgttggggacacc gattacaaccctccctcaagagtcgagtctccatgtcagcagccacgtccaagaaccaggtcttcctgcagctgacctctgtgaccgctgcggacaccgcctattatttctgt gcgacactgagagggggtactttcgactac IGG1 29 209 14 15 9 16 5 13 4 4 14 7 JY8QFUQ01A9E7L 16191 productive Homsap IGHV1-18*01 F 948 93.93 201/214 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt NA 8 8 8 8.8.8 [0.17.38.11] CARGDYFDHW in-frame + tggttacacctttaccagctatggtctcagctgggtgcgacaggcccctggccaagggcttgagtggatgggatggatcttcgtttttaacggtaacacaaaatatgcacagcacctccagggcagagtcaccatgaccacagacacatccacggacacagcctacatggagctgaggagcctgagatctgacgacacggccgtgtattactgtgcgagaggtgactactttgaccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [269] a115>c,I39>L(+ + +)|a141>c|a169>t,S57>F(- - -)|g170>t,S57>F(- - -)|c173>t,A58>V(+ - +)|a176>t,Y59>F(- + -)|c177>t,Y59>F(- + -)|t186>c|c198>a,N66>K(+ - -)|a208>c,K70>H(- - +)|g210>c,K70>H(- - +)|a253>g,S85>D(- - -)|g254>a,S85>D(- - -)| a115>c,I39>L(+ + +)|a141>c| a169>t,S57>F(- - -)|g170>t,S57>F(- - -)|c173>t,A58>V(+ - +)|a176>t,Y59>F(- + -)|c177>t,Y59>F(- + -)|t186>c| c198>a,N66>K(+ - -)|a208>c,K70>H(- - +)|g210>c,K70>H(- - +)|a253>g,S85>D(- - -)|g254>a,S85>D(- - -)| a320>g| 319 (320) 221 (222) 208 13 (14) 2 (3) 11 1 (2) 1 2 1 3 1 2 0 1 0 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 0 0 0 2 0 0 0 0 0 0 0 30 24 18 6 1 5 0 0 2 1 0 0 2 0 0 0 1 0 117 114 109 5 0 5 1 1 0 0 1 1 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|ta,175-176(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,5-6(FR1)|tt,75-76(FR1)|tt,81-82(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|tt,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,3-6(FR1)|agct,8-11(FR1)|agct,24-27(FR1)|ggct,72-75(FR1)|ggtt,79-82(CDR1)|agct,106-109(CDR1)|ggta,112-115(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)| agct,8-11(FR1)|agct,24-27(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|agct,209-212(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggttacacctttaccagctatggt ctcagctgggtgcgacaggcccctggccaagggcttgagtggatgggatgg atcttcgtttttaacggtaacaca aaatatgcacagcacctccagggcagagtcaccatgaccacagacacatccacggacacagcctacatggagctgaggagcctgagatctgacgacacggccgtgtattactgt gcgagaggtgactactttgaccac ARGDYFDH IGHV1-18 IGHJ4 ggttacacctttaccagctatggt ctcagctgggtgcgacaggcccctggccaagggcttgagtggatgggatgg atcttcgtttttaacggtaacaca aaatatgcacagcacctccagggcagagtcaccatgaccacagacacatccacggacacagcctacatggagctgaggagcctgagatctgacgacacggccgtgtattactgt gcgagaggtgactactttgaccac IGA1 13 213 5 8 3 6 2 7 1 1 6 5 JY8QFUQ01A9FXS 8072 productive (see comment) Homsap IGHV3-48*03 F 864 88.94 193/217 nt 88.48 192/217 nt Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD3-3*01 F 2 8 8 13 8.8.13 [1.16.38.11] CARDKGWSGYGMDVW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 45 of V-REGION: 1 nucleotides (from position 49 in the user submitted sequence), (cause frameshift) cctctggattcaccttcagttcttatgccatgaactgggtccgcctggtccaggcaaggggctggaatggctttcatttattggtaatactggtagtgtcatatactacgcagactctgtgaaggggcgattcaccatctccagagacaatgccaagaactcaatgtctctacaaatgagcagcctgagagccgaggacacggctctatattattgtgcgagagataaaggttggagcggatacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [287, 286] a106>t|g107>c|a113>c,E38>A(- - -)|a114>c,E38>A(- - -)|a131>t,Q44>L(- - -)|g141>c|g153>a|g157>c,V53>L(+ - +)|a164>t,Y55>F(- + -)|c165>t,Y55>F(- + -)|a169>g,S57>G(+ + -)|g173>a,S58>N(- - -)|g176>c,S59>T(+ - +)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)|c222>g|c246>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|a275>g,N92>S(- - -)|g301>c,V101>L(+ - +)|t303>a,V101>L(+ - +)|c309>t| a106>t|g107>c|a113>c,E38>A(- - -)|a114>c,E38>A(- - -)| a131>t,Q44>L(- - -)|g141>c|g153>a|g157>c,V53>L(+ - +)|a164>t,Y55>F(- + -)|c165>t,Y55>F(- + -)| a169>g,S57>G(+ + -)|g173>a,S58>N(- - -)|g176>c,S59>T(+ - +)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)| c222>g|c246>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|a275>g,N92>S(- - -)|g301>c,V101>L(+ - +)|t303>a,V101>L(+ - +)|c309>t| 320 225 201 24 8 16 3 3 4 0 3 1 3 1 5 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 0 0 0 2 0 1 0 1 0 0 0 51 50 44 6 2 4 0 1 1 0 0 0 2 0 2 0 0 0 30 24 19 5 0 5 2 1 1 0 0 0 0 0 1 0 0 0 117 114 105 9 4 5 1 1 2 0 1 1 0 1 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,113-114(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagttcttatgcc atgaactgggtccgcctggtccaggcaaggggctggaatggctttcattt attggtaatactggtagtgtcata tactacgcagactctgtgaaggggcgattcaccatctccagagacaatgccaagaactcaatgtctctacaaatgagcagcctgagagccgaggacacggctctatattattgt gcgagagataaaggttggagcggatacggtatggacgtc ARDKGWSGYGMDV IGHV3-48 IGHD3-3 IGHJ6 ggattcaccttcagttcttatgcc atgaactgggtccgcctggtccaggcaaggggctggaatggctttcattt attggtaatactggtagtgtcata tactacgcagactctgtgaaggggcgattcaccatctccagagacaatgccaagaactcaatgtctctacaaatgagcagcctgagagccgaggacacggctctatattattgt gcgagagataaaggttggagcggatacggtatggacgtc IGA2 24 212 10 14 7 14 3 10 6 2 9 7 JY8QFUQ01A9G63 15639 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 818 86.98 187/215 nt 86.51 186/215 nt Homsap IGHJ4*02 F (see comment) 159 81.25 39/48 nt Homsap IGHD3-10*01 F 3 8 7 16 8.7.16 [1.17.38.11] CAKESLVRGVISYYFDDW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) in CDR2-IMGT, from codon 58 of V-REGION: 3 nucleotides (from position 87 in the user submitted sequence), (do not cause frameshift) cctctggattcaactttggcatctataccatggcctgggtccgccaggctccagggaaggggctggagtgggtctcagctattcgtgatcatgatagcacatactacgcagactccgtgcagggccggtttttcatctcgagagacaatttcaataatacattgtatctgcaaatggatggcctgcgagccgacgacacggccgtctattactgtgcgaaagagtctctggttcggggagtcatctcatactactttgatgactggggccaggggaccctggtcaccgtcatcctccgcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccagg NA IGA2 79 95 [295, 294] c86>a,T29>N(- + -)|a103>g,S35>G(+ + -)|g107>t,S36>I(- - -)|g112>a,A38>T(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a169>c,S57>R(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g184>c,G62>H(+ - -)|g185>a,G62>H(+ - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|c228>t|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|c237>g|c248>t,S83>F(- - -)|g252>t,K84>N(+ - -)|c255>t|g258>a|c259>t|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|a277>g,S93>G(+ + -)|a283>c|g291>c,E97>D(+ - +)|a303>c| c86>a,T29>N(- + -)|a103>g,S35>G(+ + -)|g107>t,S36>I(- - -)|g112>a,A38>T(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)| a169>c,S57>R(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g184>c,G62>H(+ - -)|g185>a,G62>H(+ - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|c228>t|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|c237>g|c248>t,S83>F(- - -)|g252>t,K84>N(+ - -)|c255>t|g258>a|c259>t|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|a277>g,S93>G(+ + -)|a283>c|g291>c,E97>D(+ - +)|a303>c| 320 223 195 28 7 21 5 5 6 0 4 1 1 0 3 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 0 0 0 1 0 0 0 0 1 0 51 51 49 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 30 21 15 6 0 6 1 3 0 0 1 0 0 0 1 0 0 0 117 114 98 16 7 9 2 1 6 0 3 0 1 0 1 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaactttggcatctatacc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcagct attcgtgatcatgatagcaca tactacgcagactccgtgcagggccggtttttcatctcgagagacaatttcaataatacattgtatctgcaaatggatggcctgcgagccgacgacacggccgtctattactgt gcgaaagagtctctggttcggggagtcatctcatactactttgatgac AKESLVRGVISYYFDD IGHV3-23 IGHD3-10 IGHJ4 ggattcaactttggcatctatacc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcagct attcgtgatcatgatagcaca tactacgcagactccgtgcagggccggtttttcatctcgagagacaatttcaataatacattgtatctgcaaatggatggcctgcgagccgacgacacggccgtctattactgt gcgaaagagtctctggttcggggagtcatctcatactactttgatgac IGA2 28 210 16 12 11 18 5 10 7 0 11 10 JY8QFUQ01A9GRS 8183 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 940 93.06 201/216 nt 92.59 200/216 nt Homsap IGHJ5*02 F 183 84.31 43/51 nt Homsap IGHD5-24*01 ORF 1 8 7 12 8.7.12 [2.17.38.11] CARAPRTATLLDPW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 38 of V-REGION: 'C' (1 nucleotides at position 28 to 28 in the user submitted sequence), (cause frameshift); gtctctggcgactccatcagtggtcacCtactggagctggatcaggcagcccccagggaagggactgcagtggattggttacatctatcacagtgggagcaccaactacaacccctccctcgagagtcgagtctccatttcagtagacacgtccaagaaccagttctccctgaggttgagttctgtgaccgctgcggacacggccgtgtattactgtgcgagagccccgaggacggctacacttctcgacccctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 71 100 [285] t81>c|g83>a,G28>D(- - -)|a106>g,S36>G(+ + -)|t109>c,Y37>H(+ - -)|c127>a|g151>c,E51>Q(+ + -)|g162>t|t165>c|t172>c,Y58>H(+ - -)|a214>g,K72>E(+ - -)|a229>t,T77>S(+ - +)|a234>t|a269>g,K90>R(+ + +)|c271>t|c276>t| t81>c|g83>a,G28>D(- - -)|a106>g,S36>G(+ + -)|t109>c,Y37>H(+ - -)| c127>a|g151>c,E51>Q(+ + -)|g162>t|t165>c| t172>c,Y58>H(+ - -)| a214>g,K72>E(+ - -)|a229>t,T77>S(+ - +)|a234>t|a269>g,K90>R(+ + +)|c271>t|c276>t| a320>c| 319 (320) 223 (224) 208 15 (16) 7 (8) 8 3 1 2 4 0 (1) 1 2 0 1 0 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 0 2 0 0 0 0 0 0 0 0 51 51 47 4 3 1 0 0 0 1 0 1 0 0 1 0 1 0 30 21 20 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 117 114 108 6 3 3 2 0 2 0 0 0 2 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct ggcgactccatcagtggtcactac tggagctggatcaggcagcccccagggaagggactgcagtggattggttac atctatcacagtgggagcacc aactacaacccctccctcgagagtcgagtctccatttcagtagacacgtccaagaaccagttctccctgaggttgagttctgtgaccgctgcggacacggccgtgtattactgt gcgagagccccgaggacggctacacttctcgacccc ARAPRTATLLDP IGHV4-59 IGHD5-24 IGHJ5 ggcgactccatcagtggtcactac tggagctggatcaggcagcccccagggaagggactgcagtggattggttac atctatcacagtgggagcacc aactacaacccctccctcgagagtcgagtctccatttcagtagacacgtccaagaaccagttctccctgaggttgagttctgtgaccgctgcggacacggccgtgtattactgt gcgagagccccgaggacggctacacttctcgacccc IGG2 15 210 10 5 3 6 7 9 6 1 4 4 JY8QFUQ01A9HIP 9636 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 829 86.76 190/219 nt NA Homsap IGHJ2*01 F (see comment) 130 71.7 38/53 nt Homsap IGHD3-10*01 F 2 8 8 14 8.8.14 [2.17.38.11] CVKEDHDSGLRVLLEW in-frame - Other possibilities: Homsap_IGHJ4*02 and Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ggctctggattcacgtttggcatctatgccatgagttgggtccgccaggctccagggagggggctggagtgggtcgcaagcatgggtaatagtgctggcagtacatactacgcaggctccgtgaagggtcgcttcaacatctccagagacaattccaagaaaaccctgtatcttcaaatggacagcctgagagtcgacgacacggccagatattactgtgtgaaagaagaccatgattcgggcctcagggtcctccttgagtggggccggggaaccctggtgactgtctccgcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [292] c74>g,A25>G(- + -)|c87>g|a103>g,S35>G(+ + -)|g107>t,S36>I(- - -)|c120>t|a143>g,K48>R(+ + +)|t160>g,S54>A(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t165>c,A55>S(- + -)|t168>g,I56>M(+ + -)|a169>g,S57>G(+ + -)|g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|g185>c,G62>A(- + -)|t189>c|c192>t|a206>g,D69>G(- - -)|c222>t|g225>c|c230>a,T77>N(- + -)|c255>a,N85>K(+ - -)|g258>c|g267>t|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)|c314>t,A105>V(+ - +)| c74>g,A25>G(- + -)| c87>g|a103>g,S35>G(+ + -)|g107>t,S36>I(- - -)| c120>t|a143>g,K48>R(+ + +)|t160>g,S54>A(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t165>c,A55>S(- + -)| t168>g,I56>M(+ + -)|a169>g,S57>G(+ + -)|g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|g185>c,G62>A(- + -)|t189>c|c192>t| a206>g,D69>G(- - -)|c222>t|g225>c|c230>a,T77>N(- + -)|c255>a,N85>K(+ - -)|g258>c|g267>t|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)| c314>t,A105>V(+ - +)| 320 227 197 30 8 22 5 4 5 2 0 2 0 0 4 3 2 3 78 6 5 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 36 24 21 3 1 2 1 0 0 0 0 0 0 0 0 1 1 0 51 51 45 6 1 5 1 1 1 1 0 0 0 0 0 1 0 1 30 24 17 7 2 5 1 2 1 1 0 0 0 0 1 0 0 1 117 114 102 12 4 8 2 1 2 0 0 2 0 0 3 0 1 1 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ggctct ggattcacgtttggcatctatgcc atgagttgggtccgccaggctccagggagggggctggagtgggtcgcaagc atgggtaatagtgctggcagtaca tactacgcaggctccgtgaagggtcgcttcaacatctccagagacaattccaagaaaaccctgtatcttcaaatggacagcctgagagtcgacgacacggccagatattactgt gtgaaagaagaccatgattcgggcctcagggtcctccttgag VKEDHDSGLRVLLE IGHV3-23 IGHD3-10 IGHJ2 ggattcacgtttggcatctatgcc atgagttgggtccgccaggctccagggagggggctggagtgggtcgcaagc atgggtaatagtgctggcagtaca tactacgcaggctccgtgaagggtcgcttcaacatctccagagacaattccaagaaaaccctgtatcttcaaatggacagcctgagagtcgacgacacggccagatattactgt gtgaaagaagaccatgattcgggcctcagggtcctccttgag IGA2 28 213 15 13 8 18 7 10 5 3 13 7 JY8QFUQ01A9JOS 18501 productive Homsap IGHV3-11*01 F 954 93.55 203/217 nt NA Homsap IGHJ6*02 F 238 87.1 54/62 nt Homsap IGHD1-26*01 F 3 8 8 16 8.8.16 [1.17.38.11] CARDGDDYRSYYYGMDVW in-frame + ctctggattcaccttcagtgactactacatgagctggatccgccaggctccagggaaggggctggagtggatttcacacattagtagtagtggtagcatggtatacctggcagactctgtgaagggccgattcatcatctccagggacaacggcgagaactcactgtatcttcaaatgaacagcctgagagccgaagacacggccgtgtattactgtgcgagagatggggacgactacaggtcgtactactatggcatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [297] g157>a,V53>I(+ - +)|t163>c,Y55>H(+ - -)|t189>c|c191>t,T64>M(- - -)|c192>g,T64>M(- - -)|a193>g,I65>V(+ - +)|t199>c,Y67>L(- - -)|a200>t,Y67>L(- - -)|c201>g,Y67>L(- - -)|c230>t,T77>I(- - -)|c248>g,A83>G(- + -)|a250>g,K84>E(+ - -)|g267>t|g291>a| g157>a,V53>I(+ - +)|t163>c,Y55>H(+ - -)| t189>c|c191>t,T64>M(- - -)|c192>g,T64>M(- - -)|a193>g,I65>V(+ - +)| t199>c,Y67>L(- - -)|a200>t,Y67>L(- - -)|c201>g,Y67>L(- - -)|c230>t,T77>I(- - -)|c248>g,A83>G(- + -)|a250>g,K84>E(+ - -)|g267>t|g291>a| 320 225 211 14 3 11 2 2 2 3 0 0 1 0 0 3 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 30 24 20 4 1 3 1 0 1 1 0 0 0 0 0 1 0 0 117 114 106 8 2 6 1 1 1 1 0 0 1 0 0 2 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgactactac atgagctggatccgccaggctccagggaaggggctggagtggatttcacac attagtagtagtggtagcatggta tacctggcagactctgtgaagggccgattcatcatctccagggacaacggcgagaactcactgtatcttcaaatgaacagcctgagagccgaagacacggccgtgtattactgt gcgagagatggggacgactacaggtcgtactactatggcatggacgtc ARDGDDYRSYYYGMDV IGHV3-11 IGHD1-26 IGHJ6 ggattcaccttcagtgactactac atgagctggatccgccaggctccagggaaggggctggagtggatttcacac attagtagtagtggtagcatggta tacctggcagactctgtgaagggccgattcatcatctccagggacaacggcgagaactcactgtatcttcaaatgaacagcctgagagccgaagacacggccgtgtattactgt gcgagagatggggacgactacaggtcgtactactatggcatggacgtc IGG1 14 213 9 5 4 8 5 6 2 1 8 3 JY8QFUQ01A9KHI 3904 productive (see comment) Homsap IGHV1-2*02 F 921 92.52 198/214 nt 91.59 196/214 nt Homsap IGHJ4*02 F (see comment) 186 87.5 42/48 nt Homsap IGHD1-26*01 F 2 8 8 16 8.8.16 [0.17.38.11] CARWGATWELLLGNLDSW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 45 of V-REGION: 'A' (1 nucleotides at position 45 to 45 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 69 of V-REGION: 'G' (1 nucleotides at position 112 to 112 in the user submitted sequence), (cause frameshift); tggatacacgttcaccgactactatgtccactgggtgcgacaggAcccctggacaagggcttgagtggatggcgtggatcaaccctaacattggtgtcaccaagtctgcacGaaaaatttcagggcagggtcaccatgaccagggacacgtccatcagcacagccttcatggagctgagcagcctgagatctgacgacacggccgtttattactgtgcgagatggggggcgacgtgggaactattattggggaatcttgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [295] c87>g|g107>a,G36>D(- - -)|a115>g,M39>V(+ - -)|g117>c,M39>V(+ - -)|g161>c,G54>A(- + -)|a162>g,G54>A(- + -)|g185>t,S62>I(- - -)|g191>t,G64>V(- - -)|a195>c|c198>g,N66>K(+ - -)|a200>c,Y67>S(+ - -)|g207>a|g210>a|a263>t,Y88>F(- + -)|g279>c,R93>S(- - -)|g303>t| c87>g|g107>a,G36>D(- - -)| a115>g,M39>V(+ - -)|g117>c,M39>V(+ - -)|g161>c,G54>A(- + -)|a162>g,G54>A(- + -)| g185>t,S62>I(- - -)|g191>t,G64>V(- - -)|a195>c| c198>g,N66>K(+ - -)|a200>c,Y67>S(+ - -)|g207>a|g210>a|a263>t,Y88>F(- + -)|g279>c,R93>S(- - -)|g303>t| g319>t|a320>g| 318 (320) 220 (222) 204 16 (18) 5 (7) 11 2 (3) 3 0 0 2 0 1 0 3 2 3 (4) 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 1 0 0 0 0 0 0 0 1 0 0 51 51 47 4 0 4 2 0 0 0 0 0 0 0 2 0 0 0 30 24 21 3 1 2 0 0 0 0 1 0 0 0 0 0 2 0 117 114 107 7 3 4 0 2 0 0 1 0 1 0 1 1 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatacacgttcaccgactactat gtccactgggtgcgacaggcccctggacaagggcttgagtggatggcgtgg atcaaccctaacattggtgtcacc aagtctgcacaaaaatttcagggcagggtcaccatgaccagggacacgtccatcagcacagccttcatggagctgagcagcctgagatctgacgacacggccgtttattactgt gcgagatggggggcgacgtgggaactattattggggaatcttgactcc ARWGATWELLLGNLDS IGHV1-2 IGHD1-26 IGHJ4 ggatacacgttcaccgactactat gtccactgggtgcgacaggcccctggacaagggcttgagtggatggcgtgg atcaaccctaacattggtgtcacc aagtctgcacaaaaatttcagggcagggtcaccatgaccagggacacgtccatcagcacagccttcatggagctgagcagcctgagatctgacgacacggccgtttattactgt gcgagatggggggcgacgtgggaactattattggggaatcttgactcc IGA1 16 213 5 11 3 11 2 5 3 2 8 3 JY8QFUQ01A9LZM 11850 productive Homsap IGHV4-4*07 F (see comment) 515 70.59 156/221 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD1-1*01 F 2 8 7 16 8.7.16 [3.17.38.11] CARERISSGGGPRHLEPW in-frame + Low V-REGION identity (70,59% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tcactggaggtccggtcaacagtaatgactgctcttggacatggatccgagagtccgccgggaggggactggagtggattggccgtgtccatatgaatggccagaccgactacaatccatccttcggcagtcgtctcgccatgtccattgacacagtcaagaatgaattctcccttcgaatggtctctgtgaccgccgcagacacggccctatattactgtgcgcgagaacgtataagttccggtggcgggccgagacaccttgaaccctggggccagggaactctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 100 [300] g68>t|t74>g,V25>G(- - -)|c75>a,V25>G(- - -)|t76>g,S26>G(+ + -)|c77>g,S26>G(+ + -)|g79>c,G27>P(+ - -)|g80>c,G27>P(+ - -)|t81>g,G27>P(+ - -)|g83>t,G28>V(- - -)|t85>a,S29>N(- - -)|c86>a,S29>N(- - -)|t89>g,I30>S(- - -)|c90>t,I30>S(- - -)|g104>a,S35>N(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t108>c,S36>D(- - -)|a110>g,Y37>C(- - -)|a113>c,Y38>S(+ - -)|c114>t,Y38>S(+ - -)|g119>c,S40>T(+ - +)|c120>a,S40>T(+ - +)|g129>a|c130>g,Q44>E(+ + -)|c133>t,P45>S(+ - -)|a143>g,K48>R(+ + +)|g162>c|a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|c173>t,T58>M(- - -)|c174>g,T58>M(- - -)|g176>a,S59>N(- - -)|g189>c|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>g,S64>Q(- - -)|a196>g,N66>D(+ + -)|c204>t|c207>a|c211>t,L71>F(+ - -)|a214>g,K72>G(- - -)|a215>g,K72>G(- - -)|g216>c,K72>G(- - -)|a225>t|g226>c,V76>L(+ - +)|a229>g,T77>A(- - -)|a237>c|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|g246>a|t247>g,S83>V(- - -)|c248>t,S83>V(- - -)|c255>t|c256>g,Q86>E(+ + -)|g258>a,Q86>E(+ + -)|g267>t|a268>c,K90>R(+ + +)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c271>a,L91>M(+ + -)|a274>g,S92>V(- - -)|g275>t,S92>V(- - -)|g291>a|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)|a316>c| g68>t|t74>g,V25>G(- - -)|c75>a,V25>G(- - -)|t76>g,S26>G(+ + -)|c77>g,S26>G(+ + -)| g79>c,G27>P(+ - -)|g80>c,G27>P(+ - -)|t81>g,G27>P(+ - -)|g83>t,G28>V(- - -)|t85>a,S29>N(- - -)|c86>a,S29>N(- - -)|t89>g,I30>S(- - -)|c90>t,I30>S(- - -)|g104>a,S35>N(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t108>c,S36>D(- - -)|a110>g,Y37>C(- - -)|a113>c,Y38>S(+ - -)|c114>t,Y38>S(+ - -)| g119>c,S40>T(+ - +)|c120>a,S40>T(+ - +)|g129>a|c130>g,Q44>E(+ + -)|c133>t,P45>S(+ - -)|a143>g,K48>R(+ + +)|g162>c| a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|c173>t,T58>M(- - -)|c174>g,T58>M(- - -)|g176>a,S59>N(- - -)|g189>c|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>g,S64>Q(- - -)| a196>g,N66>D(+ + -)|c204>t|c207>a|c211>t,L71>F(+ - -)|a214>g,K72>G(- - -)|a215>g,K72>G(- - -)|g216>c,K72>G(- - -)|a225>t|g226>c,V76>L(+ - +)|a229>g,T77>A(- - -)|a237>c|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|g246>a|t247>g,S83>V(- - -)|c248>t,S83>V(- - -)|c255>t|c256>g,Q86>E(+ + -)|g258>a,Q86>E(+ + -)|g267>t|a268>c,K90>R(+ + +)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c271>a,L91>M(+ + -)|a274>g,S92>V(- - -)|g275>t,S92>V(- - -)|g291>a|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| a316>c| 320 229 163 66 13 53 10 11 8 2 5 5 2 1 8 5 4 5 78 11 6 5 1 4 0 0 0 0 0 1 0 0 0 1 1 2 36 24 9 15 0 15 2 2 2 1 1 1 0 1 2 0 1 2 51 51 44 7 2 5 1 1 1 0 0 1 0 0 2 1 0 0 30 21 12 9 1 8 1 2 1 1 1 0 0 0 1 2 0 0 117 114 85 29 8 21 6 6 4 0 2 2 2 0 3 1 2 1 8 8 7 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tcactggaggt ccggtcaacagtaatgactgctct tggacatggatccgagagtccgccgggaggggactggagtggattggccgt gtccatatgaatggccagacc gactacaatccatccttcggcagtcgtctcgccatgtccattgacacagtcaagaatgaattctcccttcgaatggtctctgtgaccgccgcagacacggccctatattactgt gcgcgagaacgtataagttccggtggcgggccgagacaccttgaaccc ARERISSGGGPRHLEP IGHV4-4 IGHD1-1 IGHJ4 ccggtcaacagtaatgactgctct tggacatggatccgagagtccgccgggaggggactggagtggattggccgt gtccatatgaatggccagacc gactacaatccatccttcggcagtcgtctcgccatgtccattgacacagtcaagaatgaattctcccttcgaatggtctctgtgaccgccgcagacacggccctatattactgt gcgcgagaacgtataagttccggtggcgggccgagacaccttgaaccc IGA1 60 210 31 29 19 38 12 22 10 1 26 23 JY8QFUQ01A9M1O 10880 productive Homsap IGHV4-4*07 F (see comment) 557 73.49 158/215 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-16*01 F 2 8 7 14 8.7.14 [1.17.38.11] CARVRDAYTAGHFDSW in-frame + Low V-REGION identity (73,49% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tctctggtgactctgtcactatgtcttattgggcctggatacgtcagcccgccgggggaggcctggagttaattggacgaacttctgccagtccaaaagttacctacaatccctccctcaggagtcgagccaccatattcgaagacacttcaaagaatcaacttatcttgaaattggcctctgtgaccgccgcggacacggccatctactactgtgcgagggtccgtgatgcctacaccgctggacactttgactcctggggcccgggaaccctggtcaccgtctcttcggcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcgtcgcatgcctgggtccacggggnttcttcccccag NA IGA2 93 100 [288, 289] g83>a,G28>D(- - -)|c87>t|a88>g,I30>V(+ - +)|g104>c,S35>T(+ - +)|g107>t,S36>M(- - -)|t108>g,S36>M(- - -)|a110>c,Y37>S(+ - -)|c111>t,Y37>S(+ - -)|c114>t|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c126>a|g129>t|a142>g,K48>G(- - -)|a143>g,K48>G(- - -)|g144>a,K48>G(- - -)|a147>c|g155>t,W52>L(+ - -)|g156>a,W52>L(+ - -)|g162>a|t165>a|t167>c,I56>T(- - -)|c168>t,I56>T(- - -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|g187>c,G63>P(+ - -)|g188>c,G63>P(+ - -)|g189>a,G63>P(+ - -)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|c195>t,T65>V(- - -)|a197>c,N66>T(- + -)|c204>t|a215>g,K72>R(+ + +)|t227>c,V76>A(+ - +)|g234>a,M78>I(+ + -)|c236>t,S79>F(- - -)|a237>c,S79>F(- - -)|t239>a,V80>E(- + -)|g246>t|c249>a|c255>t|g258>a|t259>c,F87>L(+ - -)|c261>t,F87>L(+ - -)|t262>a,S88>I(- - -)|c263>t,S88>I(- - -)|c265>t|g270>a|c271>t|a274>g,S92>A(- + -)|g275>c,S92>A(- + -)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)|t306>c| g83>a,G28>D(- - -)|c87>t|a88>g,I30>V(+ - +)|g104>c,S35>T(+ - +)|g107>t,S36>M(- - -)|t108>g,S36>M(- - -)|a110>c,Y37>S(+ - -)|c111>t,Y37>S(+ - -)|c114>t| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c126>a|g129>t|a142>g,K48>G(- - -)|a143>g,K48>G(- - -)|g144>a,K48>G(- - -)|a147>c|g155>t,W52>L(+ - -)|g156>a,W52>L(+ - -)|g162>a|t165>a| t167>c,I56>T(- - -)|c168>t,I56>T(- - -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|g187>c,G63>P(+ - -)|g188>c,G63>P(+ - -)|g189>a,G63>P(+ - -)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|c195>t,T65>V(- - -)| a197>c,N66>T(- + -)|c204>t|a215>g,K72>R(+ + +)|t227>c,V76>A(+ - +)|g234>a,M78>I(+ + -)|c236>t,S79>F(- - -)|a237>c,S79>F(- - -)|t239>a,V80>E(- + -)|g246>t|c249>a|c255>t|g258>a|t259>c,F87>L(+ - -)|c261>t,F87>L(+ - -)|t262>a,S88>I(- - -)|c263>t,S88>I(- - -)|c265>t|g270>a|c271>t|a274>g,S92>A(- + -)|g275>c,S92>A(- + -)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)|t306>c| a318>g|a320>t| 317 (320) 220 (223) 163 (164) 57 (59) 16 (18) 41 8 (9) 10 13 4 5 3 0 (1) 3 6 0 4 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 15 9 2 7 1 1 3 0 1 0 0 0 1 0 1 1 51 51 39 12 5 7 3 3 0 0 1 1 0 1 1 0 2 0 30 21 9 12 0 12 2 2 3 1 1 1 0 0 2 0 0 0 117 114 90 24 9 15 2 4 7 3 2 1 0 2 2 0 1 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactctgtcactatgtcttat tgggcctggatacgtcagcccgccgggggaggcctggagttaattggacga acttctgccagtccaaaagtt acctacaatccctccctcaggagtcgagccaccatattcgaagacacttcaaagaatcaacttatcttgaaattggcctctgtgaccgccgcggacacggccatctactactgt gcgagggtccgtgatgcctacaccgctggacactttgactcc ARVRDAYTAGHFDS IGHV4-4 IGHD3-16 IGHJ4 ggtgactctgtcactatgtcttat tgggcctggatacgtcagcccgccgggggaggcctggagttaattggacga acttctgccagtccaaaagtt acctacaatccctccctcaggagtcgagccaccatattcgaagacacttcaaagaatcaacttatcttgaaattggcctctgtgaccgccgcggacacggccatctactactgt gcgagggtccgtgatgcctacaccgctggacactttgactcc IGA2 57 210 35 22 23 36 12 21 14 2 22 19 JY8QFUQ01A9M5C 1668 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 995 95.41 208/218 nt 94.5 206/218 nt Homsap IGHJ5*02 F 237 96.08 49/51 nt Homsap IGHD1-20*01 F 1 8 8 16 8.8.16 [2.16.38.11] CARDPVGISGSVNWFDPW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 78 of V-REGION: 'G' (1 nucleotides at position 138 to 138 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 60 in the user submitted sequence), (cause frameshift) gcctctggattcaccttcagcaaccataacatgaactgggtccgccaggctccagggaagggctggagtgggtctcatgtattggtagtagtagtagtgacatatactacgcagactcagtgaagggccgattcaccGatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaagacacggctgtgtattactgtgcgagagatccggttgggataagtggaagtgtgaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [298] t105>c|g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)|g113>a,S38>N(- - -)|c164>g,S55>C(- - -)|c165>t,S55>C(- - -)|a169>g,S57>G(+ + -)|t190>g,Y64>D(- - -)|c287>t,A96>V(+ - +)|g291>a| t105>c|g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)|g113>a,S38>N(- - -)| c164>g,S55>C(- - -)|c165>t,S55>C(- - -)| a169>g,S57>G(+ + -)|t190>g,Y64>D(- - -)| c287>t,A96>V(+ - +)|g291>a| 320 226 216 10 2 8 1 3 2 2 0 0 0 0 0 1 0 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 2 0 2 0 0 0 0 0 0 0 0 51 50 48 2 0 2 0 0 1 0 0 0 0 0 0 1 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 0 1 117 114 112 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcaccttcagcaaccataac atgaactgggtccgccaggctccagggaagggctggagtgggtctcatgt attggtagtagtagtagtgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaagacacggctgtgtattactgt gcgagagatccggttgggataagtggaagtgtgaactggttcgacccc ARDPVGISGSVNWFDP IGHV3-21 IGHD1-20 IGHJ5 ggattcaccttcagcaaccataac atgaactgggtccgccaggctccagggaagggctggagtgggtctcatgt attggtagtagtagtagtgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaagacacggctgtgtattactgt gcgagagatccggttgggataagtggaagtgtgaactggttcgacccc IGA1 10 212 8 2 5 6 3 4 1 1 3 5 JY8QFUQ01A9MMF 5373 productive Homsap IGHV3-7*01 F 990 95.39 207/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-15*01 F 2 8 8 11 8.8.11 [1.17.38.11] CARSGSGSHLDYW in-frame + ctctggattcacctttagtaggttttggatgacctgggtccgccagggtccagggaaggggctggagtgggtggccaacataaagcaagttggaaatgagagatactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcattgtatctgcaaatgaacagcctgagagtcgacgacacggctgtgtattactgtgcgagaagtggtagtgggagccacttagattactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [281] c108>g,S36>R(- - -)|a110>t,Y37>F(- + -)|g119>c,S40>T(+ - +)|c134>g,A45>G(- + -)|a176>t,D59>V(- - -)|g188>a,S63>N(- - -)|a194>g,K65>R(+ + +)|c259>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| c108>g,S36>R(- - -)|a110>t,Y37>F(- + -)| g119>c,S40>T(+ - +)|c134>g,A45>G(- + -)| a176>t,D59>V(- - -)|g188>a,S63>N(- - -)|a194>g,K65>R(+ + +)| c259>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| g319>a|a320>g| 318 (320) 223 (225) 213 10 (12) 1 (3) 9 1 (2) 1 (2) 2 0 0 0 2 0 2 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 51 51 49 2 0 2 0 0 0 0 0 0 0 0 1 1 0 0 30 24 21 3 0 3 1 1 0 0 0 0 1 0 0 0 0 0 117 114 111 3 1 2 0 0 2 0 0 0 0 0 1 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtaggttttgg atgacctgggtccgccagggtccagggaaggggctggagtgggtggccaac ataaagcaagttggaaatgagaga tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcattgtatctgcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaagtggtagtgggagccacttagattac ARSGSGSHLDY IGHV3-7 IGHD2-15 IGHJ4 ggattcacctttagtaggttttgg atgacctgggtccgccagggtccagggaaggggctggagtgggtggccaac ataaagcaagttggaaatgagaga tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcattgtatctgcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaagtggtagtgggagccacttagattac IGA1 10 213 4 6 3 7 1 3 1 0 4 5 JY8QFUQ01A9OA6 5686 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 954 93.55 203/217 nt 93.09 202/217 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-3*01 F 3 8 8 11 8.8.11 [1.17.38.11] CARFGPLTRPDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'G' (1 nucleotides at position 8 to 8 in the user submitted sequence), (cause frameshift); ctctggaGttcacctttaccaactatggcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtggtgataccacataccacgcagactccgtgcagggccgattcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagtcgaggacacggccgtttattactgtgcgagattcggcccacttactcgacctgactactggggccagggaactttggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [282] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)|c164>g,A55>G(- + -)|g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)|t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|g225>a|t246>c|g258>t|c279>t|c287>t,A96>V(+ - +)|a303>t| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)| c164>g,A55>G(- + -)| g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)| t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|g225>a|t246>c|g258>t|c279>t|c287>t,A96>V(+ - +)|a303>t| a317>g,K106>R(+ + +)|g319>t|a320>t| 316 (320) 221 (225) 207 (208) 14 (17) 5 (7) 9 (10) 0 (1) 3 2 2 1 0 1 (2) 0 2 2 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 117 114 106 8 5 3 0 1 2 2 1 0 1 0 0 0 1 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgataccaca taccacgcagactccgtgcagggccgattcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagtcgaggacacggccgtttattactgt gcgagattcggcccacttactcgacctgactac ARFGPLTRPDY IGHV3-23 IGHD3-3 IGHJ4 ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgataccaca taccacgcagactccgtgcagggccgattcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagtcgaggacacggccgtttattactgt gcgagattcggcccacttactcgacctgactac IGA2 14 213 7 7 5 10 2 4 5 0 4 5 JY8QFUQ01A9PH6 19744 productive (see comment) Homsap IGHV3-48*01 F, or Homsap IGHV3-48*02 F 1018 96.35 211/219 nt 95.89 210/219 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-22*01 F 2 8 8 13 8.8.13 [2.17.38.11] CARDLYDSSGSFDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 14 to 14 in the user submitted sequence), (cause frameshift); gcctctggattcaGccttcagcagctataacatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagtagtaggagtagtaccaaaaactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaattcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgtgcgagagatttgtatgatagtagtggttcttttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttctccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [291] t105>c|g113>a,S38>N(- - -)|t177>g,S59>R(- - -)|t194>a,I65>K(- + -)|t196>a,Y66>N(- - -)|c255>t|c287>t,A96>V(+ - +)|c309>t| t105>c|g113>a,S38>N(- - -)| t177>g,S59>R(- - -)|t194>a,I65>K(- + -)| t196>a,Y66>N(- - -)|c255>t|c287>t,A96>V(+ - +)|c309>t| 320 227 219 8 3 5 0 1 3 1 0 0 0 2 0 0 0 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 1 0 1 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 0 1 0 0 0 1 117 114 110 4 2 2 0 0 3 0 0 0 0 1 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcaccttcagcagctataac atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtagtaggagtagtaccaaa aactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaattcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgt gcgagagatttgtatgatagtagtggttcttttgactac ARDLYDSSGSFDY IGHV3-48 IGHD3-22 IGHJ4 ggattcaccttcagcagctataac atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtagtaggagtagtaccaaa aactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaattcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgt gcgagagatttgtatgatagtagtggttcttttgactac IGG1 8 213 5 3 4 4 1 4 2 1 2 3 JY8QFUQ01A9PP5 9654 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30*19 F or Homsap IGHV3-30-5*01 F or Homsap IGHV3-33*05 F 968 94.04 205/218 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-21*01 F 2 8 8 15 8.8.15 [1.17.38.11] CAKEQEPHGHYVGTDYW in-frame + cgtctggattcaccttcagtagctacggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagttgtctcatttgatggaattcttgaacactatgcagactccgtgaagggccgattcaccatctccagagacaattccaggaacacgctgtatctgcaaatgaacagcctgagagctgaggacacggccgtgtattcctgtgcgaaagaacaagaaccacatgggcattatgttggaactgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [294] c75>g|t111>c|a166>g,I56>V(+ - +)|a168>c,I56>V(+ - +)|a173>t,Y58>F(- + -)|g188>t,S63>I(- - -)|a190>c,N64>L(- - -)|a191>t,N64>L(- - -)|a193>g,K65>E(+ - -)|t196>c,Y66>H(+ - -)|a251>g,K84>R(+ + +)|t300>c|a308>c,Y103>S(+ - -)|g317>a,R106>K(+ + +)| c75>g| t111>c| a166>g,I56>V(+ - +)|a168>c,I56>V(+ - +)|a173>t,Y58>F(- + -)|g188>t,S63>I(- - -)|a190>c,N64>L(- - -)|a191>t,N64>L(- - -)|a193>g,K65>E(+ - -)| t196>c,Y66>H(+ - -)|a251>g,K84>R(+ + +)|t300>c|a308>c,Y103>S(+ - -)| g317>a,R106>K(+ + +)| 320 226 212 14 3 11 3 1 0 3 3 0 2 0 0 1 1 0 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 17 7 0 7 2 0 0 0 2 0 2 0 0 0 1 0 117 114 110 4 1 3 1 0 0 2 1 0 0 0 0 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cgtct ggattcaccttcagtagctacggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt gtctcatttgatggaattcttgaa cactatgcagactccgtgaagggccgattcaccatctccagagacaattccaggaacacgctgtatctgcaaatgaacagcctgagagctgaggacacggccgtgtattcctgt gcgaaagaacaagaaccacatgggcattatgttggaactgactac AKEQEPHGHYVGTDY IGHV3-30 IGHD2-21 IGHJ4 ggattcaccttcagtagctacggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt gtctcatttgatggaattcttgaa cactatgcagactccgtgaagggccgattcaccatctccagagacaattccaggaacacgctgtatctgcaaatgaacagcctgagagctgaggacacggccgtgtattcctgt gcgaaagaacaagaaccacatgggcattatgttggaactgactac IGA1 12 213 6 6 0 1 6 11 1 1 3 7 JY8QFUQ01A9R46 17468 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 982 94.52 207/219 nt NA Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD3-16*01 F 2 8 8 10 8.8.10 [2.17.38.11] CAKDFTYAFDLW in-frame - gcgtctggattcacgttcagtagttatggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatcatctggtatgatggaagtaatcaatattatgcagactccgtgaagggccgattcaccgtctccagagacaattccaagaacacggtgtatctacaaatgaacagcctgagagccgaggacacggctgtctattattgtgcgaaagattttacgtacgcctttgatctctggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctccgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 71 89 [280] c87>g|c108>t|g163>a,V55>I(+ - +)|t165>c,V55>I(+ - +)|a168>c|a193>c,K65>Q(+ - -)|c198>t|a232>g,I78>V(+ - +)|c259>g,L87>V(+ - +)|g267>a|g303>c|c309>t|g317>a,R106>K(+ + +)| c87>g|c108>t| g163>a,V55>I(+ - +)|t165>c,V55>I(+ - +)| a168>c|a193>c,K65>Q(+ - -)| c198>t|a232>g,I78>V(+ - +)|c259>g,L87>V(+ - +)|g267>a|g303>c|c309>t| g317>a,R106>K(+ + +)| 320 227 214 13 7 6 1 3 3 1 2 0 0 0 1 2 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 2 0 0 0 1 0 0 0 0 0 0 1 0 0 51 51 49 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 30 24 22 2 1 1 0 0 0 0 2 0 0 0 0 0 0 0 117 114 108 6 4 2 1 1 2 0 0 0 0 0 1 1 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcgtct ggattcacgttcagtagttatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatc atctggtatgatggaagtaatcaa tattatgcagactccgtgaagggccgattcaccgtctccagagacaattccaagaacacggtgtatctacaaatgaacagcctgagagccgaggacacggctgtctattattgt gcgaaagattttacgtacgcctttgatctc AKDFTYAFDL IGHV3-33 IGHD3-16 IGHJ3 ggattcacgttcagtagttatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatc atctggtatgatggaagtaatcaa tattatgcagactccgtgaagggccgattcaccgtctccagagacaattccaagaacacggtgtatctacaaatgaacagcctgagagccgaggacacggctgtctattattgt gcgaaagattttacgtacgcctttgatctc IGA1 12 213 7 5 5 8 2 4 4 3 4 1 JY8QFUQ01A9S2H 20831 productive (see comment) Homsap IGHV1-2*02 F, or Homsap IGHV1-2*04 F 804 86.45 185/214 nt 85.98 184/214 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD3-3*02 F 3 8 8 11 8.8.11 [0.17.38.11] CARGFYGDYFDSW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 45 to 45 in the user submitted sequence), (cause frameshift); tggatacaccttcaccgactacttcatacactggctgcgacaggCcccctggacaacgacttgagtggatgggatggatcaaccctaagaatggtgacacaaagtatgcacagaactttcagggccgggtcaccatgaccagggacacgtccgtcagcaccatatatgtggaggtgagcagcctggaatctgacgacgcagccacttattactgtgcgagaggcttttatggtgactactttgactcctggggccagggagccgtggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [279] g107>a,G36>D(- - -)|a113>t,Y38>F(- + -)|t114>c,Y38>F(- + -)|g117>a,M39>I(+ + -)|g124>c,V42>L(+ - +)|g145>c,G49>R(- - -)|g147>a,G49>R(- - -)|c177>g,N59>K(+ - -)|g185>a,S62>N(- - -)|g191>a,G64>D(- - -)|c198>g,N66>K(+ - -)|g210>c,K70>N(+ - -)|a223>c|a250>g,I84>V(+ - +)|a258>c|g259>a,A87>I(+ - +)|c260>t,A87>I(+ - +)|c261>a,A87>I(+ - +)|c264>t|a265>g,M89>V(+ - -)|c271>g,L91>V(+ - +)|g279>c,R93>S(- - -)|a283>g,R95>E(+ - -)|g284>a,R95>E(+ - -)|a295>g,T99>A(- - -)|g297>a,T99>A(- - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)| g107>a,G36>D(- - -)|a113>t,Y38>F(- + -)|t114>c,Y38>F(- + -)| g117>a,M39>I(+ + -)|g124>c,V42>L(+ - +)|g145>c,G49>R(- - -)|g147>a,G49>R(- - -)| c177>g,N59>K(+ - -)|g185>a,S62>N(- - -)|g191>a,G64>D(- - -)| c198>g,N66>K(+ - -)|g210>c,K70>N(+ - -)|a223>c|a250>g,I84>V(+ - +)|a258>c|g259>a,A87>I(+ - +)|c260>t,A87>I(+ - +)|c261>a,A87>I(+ - +)|c264>t|a265>g,M89>V(+ - -)|c271>g,L91>V(+ - +)|g279>c,R93>S(- - -)|a283>g,R95>E(+ - -)|g284>a,R95>E(+ - -)|a295>g,T99>A(- - -)|g297>a,T99>A(- - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)| a320>g| 319 (320) 221 (222) 192 29 (30) 3 (4) 26 4 (5) 9 2 2 2 1 1 0 4 3 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 1 0 0 1 0 0 0 0 0 51 51 47 4 0 4 0 2 0 0 0 0 0 0 2 0 0 0 30 24 21 3 0 3 0 2 0 0 0 0 0 0 0 1 0 0 117 114 95 19 3 16 4 4 2 1 2 1 0 0 2 2 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatacaccttcaccgactacttc atacactggctgcgacaggcccctggacaacgacttgagtggatgggatgg atcaaccctaagaatggtgacaca aagtatgcacagaactttcagggccgggtcaccatgaccagggacacgtccgtcagcaccatatatgtggaggtgagcagcctggaatctgacgacgcagccacttattactgt gcgagaggcttttatggtgactactttgactcc ARGFYGDYFDS IGHV1-2 IGHD3-3 IGHJ4 ggatacaccttcaccgactacttc atacactggctgcgacaggcccctggacaacgacttgagtggatgggatgg atcaaccctaagaatggtgacaca aagtatgcacagaactttcagggccgggtcaccatgaccagggacacgtccgtcagcaccatatatgtggaggtgagcagcctggaatctgacgacgcagccacttattactgt gcgagaggcttttatggtgactactttgactcc IGA1 29 213 17 12 11 20 6 9 3 0 20 6 JY8QFUQ01A9W4E 14038 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 911 90.5 200/221 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD1-1*01 F 2 10 7 8 10.7.8 [1.17.38.11] CATVDRLTYW in-frame + tctctggtgtctccatgagcagtcttacttactactggggctggattcgccagccccccgggaagggcctggagtggattgggactctcttttatagtgggagcacctactacaatccgtccctcaggagtcgagtcaccatatccgctgactcgtccaagaaccagttctccctgaacctaaggtctgtgaccgccgcagacacggctgtctatttctgtgcgacggtggatcgactgacctactggggccggggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [276] g83>t,G28>V(- - -)|c90>g,I30>M(+ + -)|a103>c,S35>L(- - -)|g104>t,S35>L(- - -)|g107>c,S36>T(+ - +)|c126>t|a138>c|g147>c|g164>c,S55>T(+ - +)|a166>c,I56>L(+ + +)|a170>t,Y57>F(- + -)|c204>t|a215>g,K72>R(+ + +)|t239>c,V80>A(+ - +)|a240>t,V80>A(+ - +)|a244>t,T82>S(+ - +)|g270>c,K90>N(+ - -)|g273>a|c276>g,S92>R(- - -)|g303>c|a308>t,Y103>F(- + -)| g83>t,G28>V(- - -)|c90>g,I30>M(+ + -)|a103>c,S35>L(- - -)|g104>t,S35>L(- - -)|g107>c,S36>T(+ - +)| c126>t|a138>c|g147>c|g164>c,S55>T(+ - +)| a166>c,I56>L(+ + +)|a170>t,Y57>F(- + -)| c204>t|a215>g,K72>R(+ + +)|t239>c,V80>A(+ - +)|a240>t,V80>A(+ - +)|a244>t,T82>S(+ - +)|g270>c,K90>N(+ - -)|g273>a|c276>g,S92>R(- - -)|g303>c|a308>t,Y103>F(- + -)| g317>c,R106>T(- - -)|a318>g,R106>T(- - -)|c319>g|a320>t| 316 (320) 225 (229) 204 21 (25) 6 (8) 15 (17) 1 (2) 1 2 1 3 0 4 (5) 0 5 (6) 2 (3) 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 25 5 0 5 0 0 0 0 1 0 0 0 1 1 2 0 51 51 47 4 3 1 0 0 1 0 1 0 0 0 2 0 0 0 30 21 19 2 0 2 0 0 0 0 1 0 1 0 0 0 0 0 117 114 104 10 3 7 1 1 1 1 0 0 3 0 2 1 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 (1) 0 0 (1) 0 (1) 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgtctccatgagcagtcttacttactac tggggctggattcgccagccccccgggaagggcctggagtggattgggact ctcttttatagtgggagcacc tactacaatccgtccctcaggagtcgagtcaccatatccgctgactcgtccaagaaccagttctccctgaacctaaggtctgtgaccgccgcagacacggctgtctatttctgt gcgacggtggatcgactgacctac ATVDRLTY IGHV4-39 IGHD1-1 IGHJ4 ggtgtctccatgagcagtcttacttactac tggggctggattcgccagccccccgggaagggcctggagtggattgggact ctcttttatagtgggagcacc tactacaatccgtccctcaggagtcgagtcaccatatccgctgactcgtccaagaaccagttctccctgaacctaaggtctgtgaccgccgcagacacggctgtctatttctgt gcgacggtggatcgactgacctac IGA1 21 216 5 16 3 12 2 9 6 0 8 7 JY8QFUQ01A9XRP 10130 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 977 94.5 206/218 nt NA Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD6-25*01 F 2 8 8 15 8.8.15 [1.17.38.11] CVRRGMVAGRYDAFDIW in-frame + cctctggattcaccttcagtagttatagcatgaactgggtccgcctggctccagggaaggggctggagtgggtctcggccattagtattactagtagttccacatattacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagacctcactgtttctgcaaatgaacagcctgagagccgaggacacggctctgtattactgtgtgagaagggggatggtagcgggcagatatgatgcttttgatatctggggccgagggactatggtcaccgtctctccagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [295] c108>t|a131>t,Q44>L(- - -)|a162>g|t163>g,S55>A(- + -)|g173>t,S58>I(- - -)|g176>c,S59>T(+ - +)|a191>c,Y64>S(+ - -)|t194>c,I65>T(- - -)|c198>t|a254>c,N85>T(- + -)|a263>t,Y88>F(- + -)|g301>c,V101>L(+ - +)|c314>t,A105>V(+ - +)| c108>t| a131>t,Q44>L(- - -)|a162>g|t163>g,S55>A(- + -)| g173>t,S58>I(- - -)|g176>c,S59>T(+ - +)|a191>c,Y64>S(+ - -)|t194>c,I65>T(- - -)| c198>t|a254>c,N85>T(- + -)|a263>t,Y88>F(- + -)|g301>c,V101>L(+ - +)| c314>t,A105>V(+ - +)|g319>a|a320>g| 318 (320) 224 (226) 211 13 (15) 3 (5) 10 1 (2) 0 (1) 3 1 2 0 2 0 2 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 48 3 1 2 1 0 0 0 0 0 1 0 0 0 0 1 30 24 20 4 0 4 0 0 0 1 1 0 0 0 1 0 1 0 117 114 110 4 1 3 0 0 1 0 1 0 1 0 1 0 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 (1) 0 (1) 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtagttatagc atgaactgggtccgcctggctccagggaaggggctggagtgggtctcggcc attagtattactagtagttccaca tattacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagacctcactgtttctgcaaatgaacagcctgagagccgaggacacggctctgtattactgt gtgagaagggggatggtagcgggcagatatgatgcttttgatatc VRRGMVAGRYDAFDI IGHV3-21 IGHD6-25 IGHJ3 ggattcaccttcagtagttatagc atgaactgggtccgcctggctccagggaaggggctggagtgggtctcggcc attagtattactagtagttccaca tattacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagacctcactgtttctgcaaatgaacagcctgagagccgaggacacggctctgtattactgt gtgagaagggggatggtagcgggcagatatgatgcttttgatatc IGG1 12 213 4 8 2 5 2 7 2 1 5 4 JY8QFUQ01A9YO5 20790 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 875 88.24 195/221 nt 87.78 194/221 nt Homsap IGHJ5*02 F 147 76.47 39/51 nt Homsap IGHD3-22*01 F 2 10 7 12 10.7.12 [1.17.38.11] CARQRYYESDGQTW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'T' (1 nucleotides at position 54 to 54 in the user submitted sequence), (cause frameshift); tctctggtggctccatcaccggaagtagttattattggggctggattcgccagTcccccagggaaggggctggagtggattggaagtatgtattacactgggagcaccgactacaacccgtccctcaagagtcgggtcacgatatccgcagacaagtcgaagagccaggtctacctgaagttggactctgtgaccgccgcagacacaggtgtttattactgcgcgagacaaaggtactatgaaagtgatggccaaacctggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 89 [289, 288] g92>c,S31>T(+ - +)|a100>g,S34>G(+ + -)|t102>a,S34>G(+ + -)|c111>t|c114>t|c126>t|g162>a|c168>g,I56>M(+ + -)|t174>c|g176>c,S59>T(+ - +)|t196>g,Y66>D(- - -)|a225>g|c231>g|t239>c,V80>A(+ - +)|c245>a,T82>K(- - -)|c249>g|a254>g,N85>S(- - -)|t259>g,F87>V(+ - -)|c263>a,S88>Y(+ - -)|c271>t|a274>g,S92>D(- - -)|g275>a,S92>D(- - -)|g297>a|c299>g,A100>G(- + -)|g303>t|t312>c| g92>c,S31>T(+ - +)|a100>g,S34>G(+ + -)|t102>a,S34>G(+ + -)|c111>t|c114>t| c126>t|g162>a| c168>g,I56>M(+ + -)|t174>c|g176>c,S59>T(+ - +)| t196>g,Y66>D(- - -)|a225>g|c231>g|t239>c,V80>A(+ - +)|c245>a,T82>K(- - -)|c249>g|a254>g,N85>S(- - -)|t259>g,F87>V(+ - -)|c263>a,S88>Y(+ - -)|c271>t|a274>g,S92>D(- - -)|g275>a,S92>D(- - -)|g297>a|c299>g,A100>G(- + -)|g303>t|t312>c| 320 229 203 26 12 14 4 3 4 3 0 2 0 1 2 4 1 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 25 5 2 3 1 0 2 0 0 0 0 1 1 0 0 0 51 51 49 2 2 0 0 1 1 0 0 0 0 0 0 0 0 0 30 21 18 3 1 2 0 0 0 1 0 0 0 0 1 1 0 0 117 114 98 16 7 9 3 2 1 2 0 2 0 0 0 3 1 2 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcaccggaagtagttattat tggggctggattcgccagcccccagggaaggggctggagtggattggaagt atgtattacactgggagcacc gactacaacccgtccctcaagagtcgggtcacgatatccgcagacaagtcgaagagccaggtctacctgaagttggactctgtgaccgccgcagacacaggtgtttattactgc gcgagacaaaggtactatgaaagtgatggccaaacc ARQRYYESDGQT IGHV4-39 IGHD3-22 IGHJ5 ggtggctccatcaccggaagtagttattat tggggctggattcgccagcccccagggaaggggctggagtggattggaagt atgtattacactgggagcacc gactacaacccgtccctcaagagtcgggtcacgatatccgcagacaagtcgaagagccaggtctacctgaagttggactctgtgaccgccgcagacacaggtgtttattactgc gcgagacaaaggtactatgaaagtgatggccaaacc IGA1 26 216 14 12 7 16 7 10 9 3 9 5 JY8QFUQ01A9ZDI 5768 productive (see comment) Homsap IGHV3-33*03 F 913 91.67 198/216 nt 91.2 197/216 nt Homsap IGHJ3*01 F (see comment) 160 80 40/50 nt Homsap IGHD6-19*01 F 2 8 7 15 8.7.15 [1.17.38.11] CARDGGAVAGLDVVDVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) in CDR2-IMGT, from codon 64 of V-REGION: 1 nucleotides (from position 100 in the user submitted sequence), (cause frameshift) gtctggattgcatttcaacagctatgccatgcactgggtccgccaggctccaggcaagggcctggagtgggtggcagttatatggtttgatggaagtaaaaatattacgcagactcagtgaagggccgatccaccgtctccagagacaactccaagaacacgttgtatctgcaaatgaacagcctgagagccggggacacggccgtgtattattgtgcgagagatgggggagcagtggctggtttggacgttgtggacgtctggggccaagggaccacggtcaccgtcgcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [293] c84>g,F28>L(+ - -)|a85>c,T29>H(+ - -)|c86>a,T29>H(+ - -)|c87>t,T29>H(+ - -)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g113>c,G38>A(- + -)|g147>c|a173>t,Y58>F(- + -)|c198>t|t201>c|c210>a|t227>c,F76>S(- - -)|a232>g,I78>V(+ - +)|c259>t|a290>g,E97>G(- - -)|t300>c|c309>t|a317>g,K106>R(+ + +)| c84>g,F28>L(+ - -)|a85>c,T29>H(+ - -)|c86>a,T29>H(+ - -)|c87>t,T29>H(+ - -)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g113>c,G38>A(- + -)| g147>c| a173>t,Y58>F(- + -)| c198>t|t201>c|c210>a|t227>c,F76>S(- - -)|a232>g,I78>V(+ - +)|c259>t|a290>g,E97>G(- - -)|t300>c|c309>t| a317>g,K106>R(+ + +)| 320 224 205 19 7 12 3 1 4 4 1 2 1 0 2 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 0 7 0 1 1 1 1 1 0 0 1 1 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 23 22 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 117 114 105 9 6 3 2 0 3 3 0 1 0 0 0 0 0 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|aact,244-247(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattgcatttcaacagctatgcc atgcactgggtccgccaggctccaggcaagggcctggagtgggtggcagtt atatggtttgatggaagtaaaaa tattacgcagactcagtgaagggccgatccaccgtctccagagacaactccaagaacacgttgtatctgcaaatgaacagcctgagagccggggacacggccgtgtattattgt gcgagagatgggggagcagtggctggtttggacgttgtggacgtc ARDGGAVAGLDVVDV IGHV3-33 IGHD6-19 IGHJ3 ggattgcatttcaacagctatgcc atgcactgggtccgccaggctccaggcaagggcctggagtgggtggcagtt atatggtttgatggaagtaaaaa tattacgcagactcagtgaagggccgatccaccgtctccagagacaactccaagaacacgttgtatctgcaaatgaacagcctgagagccggggacacggccgtgtattattgt gcgagagatgggggagcagtggctggtttggacgttgtggacgtc IGG1 18 212 11 7 5 10 6 8 7 0 3 8 JY8QFUQ01AA0DL 18004 productive Homsap IGHV3-30*15 F 945 93.09 202/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD5-24*01 ORF 1 8 8 16 8.8.16 [1.17.38.11] CARDELPWDTIEYYFDYW in-frame - ctctggattcacctttagtagatattccatgcactgggtccgccaggctccaggcaaggggctagagtgggtggcacttatatcatacgatggaagtagaagaatctacgcagactccgtgaagggccgattcaccatctccagagacacttccaagaacacggtgtatctgcaaatgagtagcctgagacctgaggacacggctgtgtattactgtgcgagagatgagctgccttgggatacaatagaatactactttgactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [296] c90>t|c108>a,S36>R(- - -)|g112>t,A38>S(- + -)|t114>c,A38>S(- + -)|g163>c,V55>L(+ - +)|t174>c|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)|a194>g,K65>R(+ + +)|t196>a,Y66>I(- - -)|a197>t,Y66>I(- - -)|a245>c,N82>T(- + -)|c259>g,L87>V(+ - +)|c276>t|g286>c,A96>P(- - -)| c90>t|c108>a,S36>R(- - -)|g112>t,A38>S(- + -)|t114>c,A38>S(- + -)| g163>c,V55>L(+ - +)| t174>c|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)|a194>g,K65>R(+ + +)| t196>a,Y66>I(- - -)|a197>t,Y66>I(- - -)|a245>c,N82>T(- + -)|c259>g,L87>V(+ - +)|c276>t|g286>c,A96>P(- - -)| 320 225 210 15 3 12 2 0 2 2 1 1 1 2 2 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 1 1 0 1 0 0 0 0 1 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 20 4 1 3 2 0 0 1 0 0 0 1 0 0 0 0 117 114 108 6 1 5 0 0 1 0 1 0 1 1 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agca,274-277(FR3)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtagatattcc atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcactt atatcatacgatggaagtagaaga atctacgcagactccgtgaagggccgattcaccatctccagagacacttccaagaacacggtgtatctgcaaatgagtagcctgagacctgaggacacggctgtgtattactgt gcgagagatgagctgccttgggatacaatagaatactactttgactat ARDELPWDTIEYYFDY IGHV3-30 IGHD5-24 IGHJ4 ggattcacctttagtagatattcc atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcactt atatcatacgatggaagtagaaga atctacgcagactccgtgaagggccgattcaccatctccagagacacttccaagaacacggtgtatctgcaaatgagtagcctgagacctgaggacacggctgtgtattactgt gcgagagatgagctgccttgggatacaatagaatactactttgactat IGA1 15 213 6 9 2 7 4 8 1 2 6 6 JY8QFUQ01AA0M8 20486 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*04 F or Homsap IGHV3-30*14 F or Homsap IGHV3-30*18 F or Homsap IGHV3-30-3*03 F or Homsap IGHV3-30-5*01 F 950 93.12 203/218 nt NA Homsap IGHJ6*02 F (see comment) 184 77.42 48/62 nt Homsap IGHD5-24*01 ORF 3 8 8 18 8.8.18 [1.17.38.11] CARKGPRRDGSLQQCDMDVW in-frame + Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 and Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*03 (shorter alignment but highest percentage of identity) cctctggattcatcttcagcaaccttgcgatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaattatatcatatgatggaggtattaagtactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctacaaatgaacaacctgagacttgaggacacggctgtgtattactgtgcgagaaaaggccccagaagagatggctccctacagcaatgcgatatggacgtctgggaccaagggaccacgatcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 71 95 [303, 305] c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)|t109>c,Y37>L(- - -)|a110>t,Y37>L(- - -)|g113>c,G38>A(- + -)|c114>g,G38>A(- + -)|g163>a,V55>I(+ - +)|a187>g,S63>G(+ + -)|a191>t,N64>I(- - -)|a195>g|g267>a|g278>a,S93>N(- - -)|g286>c,A96>L(+ - +)|c287>t,A96>L(+ - +)| c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)|t109>c,Y37>L(- - -)|a110>t,Y37>L(- - -)|g113>c,G38>A(- + -)|c114>g,G38>A(- + -)| g163>a,V55>I(+ - +)| a187>g,S63>G(+ + -)|a191>t,N64>I(- - -)|a195>g| g267>a|g278>a,S93>N(- - -)|g286>c,A96>L(+ - +)|c287>t,A96>L(+ - +)| g319>a| 318 (320) 224 (226) 209 (210) 15 (16) 3 (4) 12 2 4 (5) 2 2 0 0 2 0 2 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 1 6 0 1 1 2 0 0 1 0 1 1 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 2 0 0 0 0 0 1 0 0 0 0 0 117 114 110 4 1 3 0 2 1 0 0 0 0 0 1 0 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcatcttcagcaaccttgcg atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatt atatcatatgatggaggtattaag tactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctacaaatgaacaacctgagacttgaggacacggctgtgtattactgt gcgagaaaaggccccagaagagatggctccctacagcaatgcgatatggacgtc ARKGPRRDGSLQQCDMDV IGHV3-30 IGHD5-24 IGHJ6 ggattcatcttcagcaaccttgcg atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatt atatcatatgatggaggtattaag tactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctacaaatgaacaacctgagacttgaggacacggctgtgtattactgt gcgagaaaaggccccagaagagatggctccctacagcaatgcgatatggacgtc IGA2 15 213 10 5 6 9 4 6 1 2 4 8 JY8QFUQ01AA3GK 21245 productive (see comment) Homsap IGHV3-7*01 F 881 90.23 194/215 nt 89.77 193/215 nt Homsap IGHJ6*02 F 265 91.94 57/62 nt Homsap IGHD3-22*01 F 2 8 7 14 8.7.14 [1.17.38.11] CARDPSYSYYNGLDVW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 62 of V-REGION: 1 nucleotides (from position 93 in the user submitted sequence), (cause frameshift) tctaaattcacttttagtaactattggatgaattgggtccgccaggctccagcgaagggactggagtgggtggccagtataaagcaggatggggggagacatattatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaagtcactgtatctgcaaatgaacagcctgggagtcgaagacacggctgtttattactgtgcgagagatccatcttactcctactacaatggtttggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [288] g79>a,G27>K(- - -)|g80>a,G27>K(- - -)|c87>t|g107>a,S36>N(- - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g140>c,G47>A(- + -)|g147>a|a164>g,N55>S(- - -)|c165>t,N55>S(- - -)|a174>g|a187>g,S63>G(+ + -)|t189>g,S63>G(+ + -)|a194>c,K65>T(- - -)|c198>t|t203>c,V68>A(+ - +)|c255>g,N85>K(+ - -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g291>a|g303>t| g79>a,G27>K(- - -)|g80>a,G27>K(- - -)|c87>t|g107>a,S36>N(- - -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g140>c,G47>A(- + -)|g147>a|a164>g,N55>S(- - -)|c165>t,N55>S(- - -)| a174>g|a187>g,S63>G(+ + -)|t189>g,S63>G(+ + -)|a194>c,K65>T(- - -)| c198>t|t203>c,V68>A(+ - +)|c255>g,N85>K(+ - -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g291>a|g303>t| 320 223 202 21 6 15 4 6 5 1 1 0 0 0 1 1 1 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 3 1 0 0 0 0 0 0 0 0 0 51 51 45 6 1 5 1 2 2 0 0 0 0 0 1 0 0 0 30 23 19 4 1 3 2 0 0 0 1 0 0 0 0 0 0 1 117 114 107 7 3 4 1 1 2 1 0 0 0 0 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct aaattcacttttagtaactattgg atgaattgggtccgccaggctccagcgaagggactggagtgggtggccagt ataaagcaggatggggggagaca tattatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaagtcactgtatctgcaaatgaacagcctgggagtcgaagacacggctgtttattactgt gcgagagatccatcttactcctactacaatggtttggacgtc ARDPSYSYYNGLDV IGHV3-7 IGHD3-22 IGHJ6 aaattcacttttagtaactattgg atgaattgggtccgccaggctccagcgaagggactggagtgggtggccagt ataaagcaggatggggggagaca tattatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaagtcactgtatctgcaaatgaacagcctgggagtcgaagacacggctgtttattactgt gcgagagatccatcttactcctactacaatggtttggacgtc IGA1 21 212 16 5 11 14 5 7 4 2 9 6 JY8QFUQ01AA57U 13609 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 725 81.65 178/218 nt 81.19 177/218 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-6*01 F 1 8 8 13 8.8.13 [1.17.38.11] CAKDQYGSPSDFDNW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (81,65% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR2-IMGT at codon 57 of V-REGION: 'GGA' (3 nucleotides at position 84 to 86 in the user submitted sequence), (do not cause frameshift); cctcagggttcgtttttgagaaatacgccatgagttgggtccgccaggctcccggaaaggggctggagtgggtctcggctattGGAggtgttgatgatgttggcacatactacgcagcctccgtgaagggtcggttcaccatatccagagacgattccagggagattctctatctacaaatgagtaacctgagagtcgacgatacggccgtctattactgtgcgaaagatcaatatgggagcccatccgactttgacaactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttccccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccg NA IGA1 100 95 [292, 291] t78>a|a81>g|a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|c87>t,T29>V(- - -)|a103>g,S35>E(- - -)|g104>a,S35>E(- - -)|c105>g,S35>E(- - -)|g107>a,S36>K(- - -)|c108>a,S36>K(- - -)|t111>c|c120>t|a138>c|g141>a|a162>g|a169>g,S57>G(+ + -)|g173>t,G58>V(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g185>a,G62>D(- - -)|g188>t,G63>V(- - -)|a190>g,S64>G(+ + -)|a206>c,D69>A(- - -)|c222>t|c234>a|a244>g,N82>D(+ + -)|a251>g,K84>R(+ + +)|a253>g,N85>E(+ - -)|c255>g,N85>E(+ - -)|c257>t,T86>I(- - -)|g258>t,T86>I(- - -)|g261>c|g267>a|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c294>t|a303>c| t78>a| a81>g|a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|c87>t,T29>V(- - -)|a103>g,S35>E(- - -)|g104>a,S35>E(- - -)|c105>g,S35>E(- - -)|g107>a,S36>K(- - -)|c108>a,S36>K(- - -)|t111>c| c120>t|a138>c|g141>a|a162>g| a169>g,S57>G(+ + -)|g173>t,G58>V(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g185>a,G62>D(- - -)|g188>t,G63>V(- - -)|a190>g,S64>G(+ + -)| a206>c,D69>A(- - -)|c222>t|c234>a|a244>g,N82>D(+ + -)|a251>g,K84>R(+ + +)|a253>g,N85>E(+ - -)|c255>g,N85>E(+ - -)|c257>t,T86>I(- - -)|g258>t,T86>I(- - -)|g261>c|g267>a|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c294>t|a303>c| 320 226 186 40 13 27 11 7 8 1 3 2 0 1 2 2 3 0 78 5 4 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 14 10 2 8 3 2 2 1 0 1 0 0 0 1 0 0 51 51 47 4 4 0 1 1 1 0 1 0 0 0 0 0 0 0 30 24 17 7 0 7 3 2 0 0 0 0 0 0 0 0 2 0 117 114 96 18 6 12 4 2 5 0 2 1 0 0 2 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctca gggttcgtttttgagaaatacgcc atgagttgggtccgccaggctcccggaaaggggctggagtgggtctcggct attggtgttgatgatgttggcaca tactacgcagcctccgtgaagggtcggttcaccatatccagagacgattccagggagattctctatctacaaatgagtaacctgagagtcgacgatacggccgtctattactgt gcgaaagatcaatatgggagcccatccgactttgacaac AKDQYGSPSDFDN IGHV3-23 IGHD6-6 IGHJ4 gggttcgtttttgagaaatacgcc atgagttgggtccgccaggctcccggaaaggggctggagtgggtctcggct attggtgttgatgatgttggcaca tactacgcagcctccgtgaagggtcggttcaccatatccagagacgattccagggagattctctatctacaaatgagtaacctgagagtcgacgatacggccgtctattactgt gcgaaagatcaatatgggagcccatccgactttgacaac IGA1 39 213 27 12 15 24 12 15 10 2 12 15 JY8QFUQ01AA63Y 6913 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 801 85.71 186/217 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD1-26*01 F 1 8 8 13 8.8.13 [1.17.38.11] CAKDAVGLTRLFGDW in-frame + ctctggatttgtctttagtagatatgccatggcctgggtccgccaggctccagggcaggggctggagtgggtcgccagtattggcgggagtggtgataacacatactacgcggactccgtgaagggccggttcaccatctccagagacaactccaataacaaactgtttctgcaaatggacagtttgcgagccggggacacggccagatatttctgtgcgaaagatgcggtgggacttacccgactctttggtgactggggccagggaaccgtggtcagcgccgcctctgcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtccatcgcctgccctggtccagggcttcttccccca NA IGA1 86 89 [287] c84>t|a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|c105>t|c108>a,S36>R(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a142>c,K48>Q(+ - -)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|a169>g,S57>G(+ + -)|t171>c,S57>G(+ + -)|t174>g|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|a204>g|t246>c|g252>t,K84>N(+ - -)|c257>a,T86>K(- - -)|g258>a,T86>K(- - -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|c280>t|a283>c|a290>g,E97>G(- - -)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)|a308>t,Y103>F(- + -)| c84>t|a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|c105>t|c108>a,S36>R(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a142>c,K48>Q(+ - -)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| a169>g,S57>G(+ + -)|t171>c,S57>G(+ + -)|t174>g|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)| a204>g|t246>c|g252>t,K84>N(+ - -)|c257>a,T86>K(- - -)|g258>a,T86>K(- - -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|c280>t|a283>c|a290>g,E97>G(- - -)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)|a308>t,Y103>F(- + -)| 320 225 194 31 8 23 6 5 5 2 3 2 2 0 1 1 1 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 1 0 3 0 0 1 0 0 0 0 0 0 51 51 44 7 0 7 1 1 0 0 2 0 0 0 1 1 0 1 30 24 19 5 1 4 1 2 0 1 0 0 0 0 0 0 0 1 117 114 100 14 5 9 3 2 2 1 1 1 2 0 0 0 1 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatttgtctttagtagatatgcc atggcctgggtccgccaggctccagggcaggggctggagtgggtcgccagt attggcgggagtggtgataacaca tactacgcggactccgtgaagggccggttcaccatctccagagacaactccaataacaaactgtttctgcaaatggacagtttgcgagccggggacacggccagatatttctgt gcgaaagatgcggtgggacttacccgactctttggtgac AKDAVGLTRLFGD IGHV3-23 IGHD1-26 IGHJ4 ggatttgtctttagtagatatgcc atggcctgggtccgccaggctccagggcaggggctggagtgggtcgccagt attggcgggagtggtgataacaca tactacgcggactccgtgaagggccggttcaccatctccagagacaactccaataacaaactgtttctgcaaatggacagtttgcgagccggggacacggccagatatttctgt gcgaaagatgcggtgggacttacccgactctttggtgac IGA1 31 213 18 13 10 15 8 16 5 3 16 7 JY8QFUQ01AA9O7 16825 productive (see comment) Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 859 88.89 192/216 nt 88.43 191/216 nt Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD5-18*01 F 3 8 7 21 8.7.21 [1.17.38.11] CAKDSRAGGYPNGYFYYSGLDVW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 63 of V-REGION: 1 nucleotides (from position 96 in the user submitted sequence), (cause frameshift) ctctggacgctccttgagaagctttggcatgcactgggtccgccaggctccaggcaagggactggagtgggtggcacttacttcgtatgacggaataggaaatattatgcagactccgtgaagggccgattcaccatctccagagacaactccaagaatacgttatttctgcaaatggacagtctgagagctgaggacacggctctttattactgtgcgaaagattcgcgagcgggtggttatccaaatggatacttctattactctggtctggacgtctggggccaagggacaatagtcaccgtctcctcagcctccaccaagggcccatcggtcttcccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [312, 311] t82>c,F28>R(- - -)|t83>g,F28>R(- - -)|a85>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|t105>a,S35>R(- - -)|a110>t,Y37>F(- + -)|g147>a|g163>c,V55>L(+ - +)|t167>c,I56>T(- - -)|a168>t,I56>T(- - -)|a171>g|t177>c|a191>g,N64>R(+ - -)|t192>g,N64>R(+ - -)|c198>t|t246>c|c255>t|c259>t|g261>a|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)|g317>a,R106>K(+ + +)| t82>c,F28>R(- - -)|t83>g,F28>R(- - -)|a85>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|t105>a,S35>R(- - -)|a110>t,Y37>F(- + -)| g147>a|g163>c,V55>L(+ - +)| t167>c,I56>T(- - -)|a168>t,I56>T(- - -)|a171>g|t177>c|a191>g,N64>R(+ - -)|t192>g,N64>R(+ - -)| c198>t|t246>c|c255>t|c259>t|g261>a|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)| g317>a,R106>K(+ + +)| 320 224 199 25 9 16 3 3 4 4 0 0 4 1 2 1 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 0 0 1 0 0 2 1 0 1 0 1 51 51 49 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 30 23 17 6 2 4 2 0 0 2 0 0 1 0 0 0 0 1 117 114 104 10 6 4 1 1 4 1 0 0 1 0 1 0 1 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggacgctccttgagaagctttggc atgcactgggtccgccaggctccaggcaagggactggagtgggtggcactt acttcgtatgacggaataggaaa tattatgcagactccgtgaagggccgattcaccatctccagagacaactccaagaatacgttatttctgcaaatggacagtctgagagctgaggacacggctctttattactgt gcgaaagattcgcgagcgggtggttatccaaatggatacttctattactctggtctggacgtc AKDSRAGGYPNGYFYYSGLDV IGHV3-30 IGHD5-18 IGHJ6 ggacgctccttgagaagctttggc atgcactgggtccgccaggctccaggcaagggactggagtgggtggcactt acttcgtatgacggaataggaaa tattatgcagactccgtgaagggccgattcaccatctccagagacaactccaagaatacgttatttctgcaaatggacagtctgagagctgaggacacggctctttattactgt gcgaaagattcgcgagcgggtggttatccaaatggatacttctattactctggtctggacgtc IGG1 24 212 13 11 6 10 7 14 7 2 5 10 JY8QFUQ01AA9UM 10798 productive Homsap IGHV4-61*02 F 1010 95.48 211/221 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-3*02 F 2 10 7 15 10.7.15 [1.17.38.11] CARAGKQDFWSGYSDDW in-frame + tctctggtggctccatcggcagtggtagttattactggagctggatccggcagcccgccgggaagggactggaataccttgggcgtatctataccagtgggagcaccaactacaacccctccctcaagagtcgagtcaccatttcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgccgctgacacggccatttattactgtgcgagagcggggaaacaggatttttggagtggttattctgacgactggggccagggaaccctggtcatagtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [298] a91>g,S31>G(+ + -)|c111>t|g153>a|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|a157>c,I53>L(+ + +)|a234>t|a291>t|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| a91>g,S31>G(+ + -)|c111>t| g153>a|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|a157>c,I53>L(+ + +)| a234>t|a291>t|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| a320>c| 319 (320) 228 (229) 218 10 (11) 4 (5) 6 1 3 1 0 1 (2) 0 2 0 1 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 1 1 1 0 1 0 0 0 0 0 0 0 0 0 51 51 47 4 1 3 0 2 0 0 1 0 0 0 1 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 110 4 2 2 0 1 0 0 0 0 2 0 0 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcggcagtggtagttattac tggagctggatccggcagcccgccgggaagggactggaataccttgggcgt atctataccagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatttcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgccgctgacacggccatttattactgt gcgagagcggggaaacaggatttttggagtggttattctgacgac ARAGKQDFWSGYSDD IGHV4-61 IGHD3-3 IGHJ4 ggtggctccatcggcagtggtagttattac tggagctggatccggcagcccgccgggaagggactggaataccttgggcgt atctataccagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatttcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgccgctgacacggccatttattactgt gcgagagcggggaaacaggatttttggagtggttattctgacgac IGG1 10 216 5 5 4 6 1 4 3 1 5 1 JY8QFUQ01AAQL2 14957 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 925 90.99 202/222 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD2-21*02 F 1 10 7 12 10.7.12 [2.17.38.11] CAWGWFGLNYFDYW in-frame - gtctctggtggctccgtcagaagtaccagttactactggggctggatccgccagcccccagggaaggggctggagtggattagtaacattcattctagtggaaccacctactacaacccgtccctcaacagtcgagtcaccatgtccgtaaacacgtccaagaaccagttctccctgaggctgagttctgtgaccgccgcggacacggctgtatattactgtgcatgggggtggttcggactgaactactttgactactggggcccgggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [289] a88>g,I30>V(+ - +)|c93>a,S31>R(- - -)|g104>c,S35>T(+ - +)|t105>c,S35>T(+ - +)|g160>a,G54>S(+ + -)|g162>t,G54>S(+ + -)|g164>a,S55>N(- - -)|t165>c,S55>N(- - -)|c168>t|t169>c,Y57>H(+ - -)|a173>c,Y58>S(+ - -)|g189>a|g191>c,S64>T(+ - +)|g216>c,K72>N(+ - -)|a234>g,I78>M(+ + -)|g241>a,D81>N(+ + -)|a269>g,K90>R(+ + +)|c276>t|a291>g|g303>a| a88>g,I30>V(+ - +)|c93>a,S31>R(- - -)|g104>c,S35>T(+ - +)|t105>c,S35>T(+ - +)| g160>a,G54>S(+ + -)|g162>t,G54>S(+ + -)|g164>a,S55>N(- - -)|t165>c,S55>N(- - -)| c168>t|t169>c,Y57>H(+ - -)|a173>c,Y58>S(+ - -)|g189>a|g191>c,S64>T(+ - +)| g216>c,K72>N(+ - -)|a234>g,I78>M(+ + -)|g241>a,D81>N(+ + -)|a269>g,K90>R(+ + +)|c276>t|a291>g|g303>a| g315>a|a316>t,R106>W(- - -)|a318>g,R106>W(- - -)|c319>g|a320>g| 314 (320) 224 (230) 204 (205) 20 (25) 5 (8) 15 (17) 4 (6) 5 (6) 2 3 1 1 0 (1) 0 3 0 (1) 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 0 4 1 0 0 1 0 1 0 0 1 0 0 0 51 51 47 4 0 4 0 2 0 1 0 0 0 0 0 0 1 0 30 21 16 5 2 3 0 1 1 1 1 0 0 0 1 0 0 0 117 114 107 7 3 4 3 2 1 0 0 0 0 0 1 0 0 0 2 (8) 2 2 (3) 0 (5) 0 (3) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 (1) 0 0 0 (1) 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct ggtggctccgtcagaagtaccagttactac tggggctggatccgccagcccccagggaaggggctggagtggattagtaac attcattctagtggaaccacc tactacaacccgtccctcaacagtcgagtcaccatgtccgtaaacacgtccaagaaccagttctccctgaggctgagttctgtgaccgccgcggacacggctgtatattactgt gcatgggggtggttcggactgaactactttgactac AWGWFGLNYFDY IGHV4-39 IGHD2-21 IGHJ4 ggtggctccgtcagaagtaccagttactac tggggctggatccgccagcccccagggaaggggctggagtggattagtaac attcattctagtggaaccacc tactacaacccgtccctcaacagtcgagtcaccatgtccgtaaacacgtccaagaaccagttctccctgaggctgagttctgtgaccgccgcggacacggctgtatattactgt gcatgggggtggttcggactgaactactttgactac IGA1 20 216 14 6 7 12 7 8 3 2 8 7 JY8QFUQ01AARBP 16631 productive Homsap IGHV3-72*01 F 1088 98.66 221/224 nt NA Homsap IGHJ5*02 F (see comment) 183 84.31 43/51 nt Homsap IGHD2-15*01 F 1 8 10 8 8.10.8 [1.17.38.11] CLRPPRYDPW in-frame - Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) cctctggattaaccctcagtgaccactacatggactgggtccgccaggctccagggaaggggctggagtgggttggccgtactagaaacaaagctaacagttacaccacagaatacgccgcgtctgtgaagggcagattcaccatctcaagagatgattcaaagaactcactgtatctgcaaatgaacagcctgaaaaccgaggacacggccgtgtattactgtcttaggccgccgaggtacgacccctggggccaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 79 95 [279, 278] c84>a,F28>L(+ - -)|t88>c,F30>L(+ - -)|a216>g|g313>c,A105>L(+ - +)|c314>t,A105>L(+ - +)| c84>a,F28>L(+ - -)|t88>c,F30>L(+ - -)| a216>g| g313>c,A105>L(+ - +)|c314>t,A105>L(+ - +)|a318>g|g319>c|a320>c| 317 (320) 229 (232) 224 5 (8) 1 (4) 4 1 (2) 0 1 1 0 (1) 1 0 0 1 (2) 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 1 0 1 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 113 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 5 (8) 5 3 2 (5) 0 (3) 2 0 (1) 0 1 0 0 (1) 0 0 0 1 (2) 0 0 0 ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,165-168(FR2-CDR2)|agct,177-180(CDR2)|aact,253-256(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tgct,312-315(FR3)| cctct ggattaaccctcagtgaccactac atggactgggtccgccaggctccagggaaggggctggagtgggttggccgt actagaaacaaagctaacagttacaccaca gaatacgccgcgtctgtgaagggcagattcaccatctcaagagatgattcaaagaactcactgtatctgcaaatgaacagcctgaaaaccgaggacacggccgtgtattactgt cttaggccgccgaggtacgacccc LRPPRYDP IGHV3-72 IGHD2-15 IGHJ5 ggattaaccctcagtgaccactac atggactgggtccgccaggctccagggaaggggctggagtgggttggccgt actagaaacaaagctaacagttacaccaca gaatacgccgcgtctgtgaagggcagattcaccatctcaagagatgattcaaagaactcactgtatctgcaaatgaacagcctgaaaaccgaggacacggccgtgtattactgt cttaggccgccgaggtacgacccc IGA2 3 219 2 1 0 1 2 2 1 0 0 2 JY8QFUQ01AARXS 16427 productive (see comment) Homsap IGHV3-33*03 F 932 92.2 201/218 nt 91.74 200/218 nt Homsap IGHJ3*01 F (see comment) 160 80 40/50 nt Homsap IGHD6-19*01 F 2 8 8 15 8.8.15 [1.17.38.11] CARDGGAVAGLDVVDVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT at codon 28 of V-REGION: 'G' (1 nucleotides at position 11 to 11 in the user submitted sequence), (cause frameshift); cgtctggattGcattttcaacagctatgccatgcactgggtccgccaggctccaggcaagggcctggagtgggtggcagttatatggtttgatggaagtaaaaaatattacgcagactcagtgaagggccgatccaccgtctccagagacaactccaagaacacgttgtatctgcaaatgaacagcctgagagccggggacacggccgtgtattattgtgcgagagatgggggagcagtggctggtttggacgttgtggacgtctggggccaagggaccacggtcaccgtcgcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [296] c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g113>c,G38>A(- + -)|g147>c|a173>t,Y58>F(- + -)|t192>a,N64>K(+ - -)|c198>t|t201>c|c210>a|t227>c,F76>S(- - -)|a232>g,I78>V(+ - +)|c259>t|a290>g,E97>G(- - -)|t300>c|c309>t|a317>g,K106>R(+ + +)| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g113>c,G38>A(- + -)| g147>c| a173>t,Y58>F(- + -)|t192>a,N64>K(+ - -)| c198>t|t201>c|c210>a|t227>c,F76>S(- - -)|a232>g,I78>V(+ - +)|c259>t|a290>g,E97>G(- - -)|t300>c|c309>t| a317>g,K106>R(+ + +)| 320 226 208 18 7 11 3 1 5 4 0 1 1 1 2 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 1 2 1 0 0 0 0 1 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 1 1 0 0 0 0 117 114 105 9 6 3 2 0 3 3 0 1 0 0 0 0 0 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|aact,244-247(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cgtct ggattcattttcaacagctatgcc atgcactgggtccgccaggctccaggcaagggcctggagtgggtggcagtt atatggtttgatggaagtaaaaaa tattacgcagactcagtgaagggccgatccaccgtctccagagacaactccaagaacacgttgtatctgcaaatgaacagcctgagagccggggacacggccgtgtattattgt gcgagagatgggggagcagtggctggtttggacgttgtggacgtc ARDGGAVAGLDVVDV IGHV3-33 IGHD6-19 IGHJ3 ggattcattttcaacagctatgcc atgcactgggtccgccaggctccaggcaagggcctggagtgggtggcagtt atatggtttgatggaagtaaaaaa tattacgcagactcagtgaagggccgatccaccgtctccagagacaactccaagaacacgttgtatctgcaaatgaacagcctgagagccggggacacggccgtgtattattgt gcgagagatgggggagcagtggctggtttggacgttgtggacgtc IGG1 17 213 12 5 6 9 6 8 7 0 3 7 JY8QFUQ01AAS9C 20734 productive Homsap IGHV2-5*02 F 1134 96.31 235/244 nt NA Homsap IGHJ6*02 F (see comment) 184 77.42 48/62 nt Homsap IGHD4-17*01 F 3 10 7 13 10.7.13 [9.17.38.11] CAHFSVTTPYGMDVW in-frame - Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) gaccctcacgctgacctgcaccttctctgggttctcactcaccactactggagtgggtgtgggctggatccgtcagcccccaggaaaggccctggagtggcttgcagtcattttttgggatgatgatgagcgccacagcccatctctgaggagaaggctcaccatcaccaaggacatctccaaaaaccaggtggtccttacaatgaccaacatggaccctgtggacacagccacatattactgtgcacacttttcggtgactaccccatatggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [315] g92>c,S31>T(+ - +)|g104>c,S35>T(+ - +)|c163>g,L55>V(+ - +)|a170>t,Y57>F(- + -)|a193>g,K65>E(+ - -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c222>a,S74>R(- - -)|c245>t,T82>I(- - -)| g92>c,S31>T(+ - +)|g104>c,S35>T(+ - +)| c163>g,L55>V(+ - +)| a170>t,Y57>F(- + -)|a193>g,K65>E(+ - -)| t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c222>a,S74>R(- - -)|c245>t,T82>I(- - -)| a319>t,R107>F(- - -)|g320>t,R107>F(- - -)|a321>t,R107>F(- - -)|c322>t| 318 (322) 250 (254) 241 9 (13) 0 (1) 9 (12) 2 0 1 (2) 1 0 1 1 (3) 0 2 1 0 (1) 0 78 28 28 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 0 2 0 0 0 0 0 0 0 0 2 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 21 19 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 117 114 110 4 0 4 1 0 1 1 0 1 0 0 0 0 0 0 6 (10) 6 6 0 (4) 0 (1) 0 (3) 0 0 0 (1) 0 0 0 0 (2) 0 0 0 0 (1) 0 aa,13-14(FR1)|aa,40-41(FR1)|ta,102-103(CDR1)|aa,141-142(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,238-239(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,267-268(FR3)|aa,270-271(FR3)|aa,277-278(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,10-11(FR1)|tt,73-74(FR1)|tt,82-83(CDR1)|ta,102-103(CDR1)|tt,158-159(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,80-83(CDR1)|ggct,118-121(FR2)|ggct,155-158(FR2)|agca,220-223(FR3)|ggct,224-227(FR3)| aacc,41-44(FR1)|agcc,131-134(FR2)|agcc,202-205(FR3)|aacc,253-256(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)| gaccctcacgctgacctgcaccttctct gggttctcactcaccactactggagtgggt gtgggctggatccgtcagcccccaggaaaggccctggagtggcttgcagtc attttttgggatgatgatgag cgccacagcccatctctgaggagaaggctcaccatcaccaaggacatctccaaaaaccaggtggtccttacaatgaccaacatggaccctgtggacacagccacatattactgt gcacacttttcggtgactaccccatatggtatggacgtc AHFSVTTPYGMDV IGHV2-5 IGHD4-17 IGHJ6 gggttctcactcaccactactggagtgggt gtgggctggatccgtcagcccccaggaaaggccctggagtggcttgcagtc attttttgggatgatgatgag cgccacagcccatctctgaggagaaggctcaccatcaccaaggacatctccaaaaaccaggtggtccttacaatgaccaacatggaccctgtggacacagccacatattactgt gcacacttttcggtgactaccccatatggtatggacgtc IGG1 9 216 4 5 1 5 3 4 0 0 5 4 JY8QFUQ01AATZ4 20244 productive Homsap IGHV1-2*02 F 826 87.79 187/213 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 3 8 8 18 8.8.18 [X.17.38.11] CARDGISMTLVRGVRPFDYW in-frame + ggatacaccttcatcggccattatatacattgggtgcgacaggcccctggacaagggcttgaatgggtggggtggatcaaccctaacagtggtgttacaaactatgcacagcagtttcaggacagggtcaccatgaccgtcgacacgtccatcagcactgcctacatggacctcaaaagtctaaagtctgacgacacggccatctattactgtgcgagagacggaataagtatgactctggttcggggagttaggcccttcgactactggggccagggaaccctggtcagcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatggaacgtggtcatcgcctgcctggtccagggcttcttcccccagga NA IGA1 100 89 [298, 297] c104>t,T35>I(- - -)|t109>c,Y37>H(+ - -)|c111>t,Y37>H(+ - -)|g117>a,M39>I(+ + -)|c120>t|g153>a|a157>g,M53>V(+ - -)|a162>g|g191>t,G64>V(- - -)|c192>t,G64>V(- - -)|a208>c,K70>Q(+ - -)|g221>a,G74>D(- - -)|a238>g,R80>V(- - -)|g239>t,R80>V(- - -)|g240>c,R80>V(- - -)|a258>t|g270>c,E90>D(+ - +)|g273>c|g275>a,S92>K(- - -)|c276>a,S92>K(- - -)|g279>t,R93>S(- - -)|g282>a|g284>a,R95>K(+ + +)|a285>g,R95>K(+ + +)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)| c104>t,T35>I(- - -)|t109>c,Y37>H(+ - -)|c111>t,Y37>H(+ - -)| g117>a,M39>I(+ + -)|c120>t|g153>a|a157>g,M53>V(+ - -)|a162>g| g191>t,G64>V(- - -)|c192>t,G64>V(- - -)| a208>c,K70>Q(+ - -)|g221>a,G74>D(- - -)|a238>g,R80>V(- - -)|g239>t,R80>V(- - -)|g240>c,R80>V(- - -)|a258>t|g270>c,E90>D(+ - +)|g273>c|g275>a,S92>K(- - -)|c276>a,S92>K(- - -)|g279>t,R93>S(- - -)|g282>a|g284>a,R95>K(+ + +)|a285>g,R95>K(+ + +)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)| 320 221 195 26 6 20 4 7 4 1 1 1 1 0 4 0 3 0 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 2 1 0 0 0 0 0 0 0 0 51 51 46 5 3 2 2 2 1 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 1 0 0 0 0 0 0 0 1 0 117 114 98 16 3 13 2 5 0 0 1 1 1 0 4 0 2 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| ggatacaccttcatcggccattat atacattgggtgcgacaggcccctggacaagggcttgaatgggtggggtgg atcaaccctaacagtggtgttaca aactatgcacagcagtttcaggacagggtcaccatgaccgtcgacacgtccatcagcactgcctacatggacctcaaaagtctaaagtctgacgacacggccatctattactgt gcgagagacggaataagtatgactctggttcggggagttaggcccttcgactac ARDGISMTLVRGVRPFDY IGHV1-2 IGHD3-10 IGHJ4 ggatacaccttcatcggccattat atacattgggtgcgacaggcccctggacaagggcttgaatgggtggggtgg atcaaccctaacagtggtgttaca aactatgcacagcagtttcaggacagggtcaccatgaccgtcgacacgtccatcagcactgcctacatggacctcaaaagtctaaagtctgacgacacggccatctattactgt gcgagagacggaataagtatgactctggttcggggagttaggcccttcgactac IGA1 26 213 16 10 11 19 5 7 6 0 15 5 JY8QFUQ01AAUM2 2968 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 868 89.35 193/216 nt 88.89 192/216 nt Homsap IGHJ3*01 F (see comment) 169 82 41/50 nt Homsap IGHD2-15*01 F 2 7 8 14 7.8.14 [1.17.38.11] CARGAGAEGKGGMDVW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT, from codon 36 of V-REGION: 1 nucleotides (from position 22 in the user submitted sequence), (cause frameshift) ctctggattcaccttcaaaagtatggcatgaactggctccgccaggctccagggaaggggctggagtgggtcgcaaccattcgcagtagtggtacttccatacactatgccgactccgtgaagggccgattcactatcaccagagacaacgccaacaactcactgtatctgcaattgaacagcctgggagtcgaggactcggctgtgtatttctgtgcgagaggggccggcgcagaaggtaaaggtggtatggacgtctggggccaagggactacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [289] g104>a,S35>K(- - -)|t105>a,S35>K(- - -)|a112>g,S38>G(+ + -)|g124>c,V42>L(+ - +)|t160>g,S54>A(- + -)|t163>a,S55>T(+ - +)|a169>c,S57>R(- - -)|t171>c,S57>R(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|a191>c,Y64>S(+ - -)|t196>c,Y66>H(+ - -)|c201>t|a204>c|a210>c|c231>t|t235>a,S79>T(+ - +)|g252>c,K84>N(+ - -)|a271>t,M91>L(+ + -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|a295>t,T99>S(+ - +)|a308>t,Y103>F(- + -)| g104>a,S35>K(- - -)|t105>a,S35>K(- - -)|a112>g,S38>G(+ + -)| g124>c,V42>L(+ - +)|t160>g,S54>A(- + -)|t163>a,S55>T(+ - +)| a169>c,S57>R(- - -)|t171>c,S57>R(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|a191>c,Y64>S(+ - -)| t196>c,Y66>H(+ - -)|c201>t|a204>c|a210>c|c231>t|t235>a,S79>T(+ - +)|g252>c,K84>N(+ - -)|a271>t,M91>L(+ + -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|a295>t,T99>S(+ - +)|a308>t,Y103>F(- + -)| a320>g| 319 (320) 223 (224) 200 23 (24) 4 (5) 19 3 (4) 1 3 2 4 0 3 3 3 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 20 3 0 3 1 1 0 0 0 0 0 1 0 0 0 0 51 51 48 3 0 3 0 0 0 0 0 0 0 1 1 0 0 1 30 24 19 5 0 5 1 0 0 1 2 0 0 0 1 0 0 0 117 114 102 12 4 8 1 0 3 1 2 0 3 1 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcaaaagtatggc atgaactggctccgccaggctccagggaaggggctggagtgggtcgcaacc attcgcagtagtggtacttccata cactatgccgactccgtgaagggccgattcactatcaccagagacaacgccaacaactcactgtatctgcaattgaacagcctgggagtcgaggactcggctgtgtatttctgt gcgagaggggccggcgcagaaggtaaaggtggtatggacgtc ARGAGAEGKGGMDV IGHV3-21 IGHD2-15 IGHJ3 ggattcaccttcaaaagtatggc atgaactggctccgccaggctccagggaaggggctggagtgggtcgcaacc attcgcagtagtggtacttccata cactatgccgactccgtgaagggccgattcactatcaccagagacaacgccaacaactcactgtatctgcaattgaacagcctgggagtcgaggactcggctgtgtatttctgt gcgagaggggccggcgcagaaggtaaaggtggtatggacgtc IGA2 23 212 9 14 4 7 5 16 4 0 11 8 JY8QFUQ01AAUWG 19692 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F (see comment) 177 85.42 41/48 nt Homsap IGHD5-12*01 F 1 8 8 9 8.8.9 [1.17.38.11] CARLPNTIDSW in-frame + Other possibilities: Homsap_IGHJ5*01 (highest number of consecutive identical nucleotides) ttctggatatagttttgccacctactggatcggctgggtgcgccagaggcccgggaagggcctggagtggatgggggtcatctatcctggtgactctgataccagatacagcccgtccttccaaggccaggtcaccatttcagccgacaagtccctcagtatcgcctacctgcagtggagcagcctgcaggcctcggacaccgccatatattactgtgcgagactgcctaacactattgactcctggggccaggggaccctggtcaacgtctcctcagcatcccctgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [276] c84>t|c87>t|a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)|t134>g,M45>R(- + -)|a144>g|a163>g,I55>V(+ - +)|c234>t|a250>c,I84>L(+ + +)|c255>t|c257>t,T86>I(- - -)|a283>c,K95>Q(+ - -)|g303>a,M101>I(+ + -)| c84>t|c87>t|a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)| t134>g,M45>R(- + -)|a144>g|a163>g,I55>V(+ - +)| c234>t|a250>c,I84>L(+ + +)|c255>t|c257>t,T86>I(- - -)|a283>c,K95>Q(+ - -)|g303>a,M101>I(+ + -)| a320>t| 319 (320) 224 (225) 211 13 (14) 5 (6) 8 3 1 5 0 2 0 0 (1) 0 1 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 1 0 2 0 0 0 0 0 1 0 0 0 51 51 48 3 1 2 2 0 0 0 0 0 0 0 0 0 0 1 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 2 4 0 1 3 0 2 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ttct ggatatagttttgccacctactgg atcggctgggtgcgccagaggcccgggaagggcctggagtggatgggggtc atctatcctggtgactctgatacc agatacagcccgtccttccaaggccaggtcaccatttcagccgacaagtccctcagtatcgcctacctgcagtggagcagcctgcaggcctcggacaccgccatatattactgt gcgagactgcctaacactattgactcc ARLPNTIDS IGHV5-51 IGHD5-12 IGHJ4 ggatatagttttgccacctactgg atcggctgggtgcgccagaggcccgggaagggcctggagtggatgggggtc atctatcctggtgactctgatacc agatacagcccgtccttccaaggccaggtcaccatttcagccgacaagtccctcagtatcgcctacctgcagtggagcagcctgcaggcctcggacaccgccatatattactgt gcgagactgcctaacactattgactcc IGA1 13 213 9 4 6 7 3 6 3 2 6 2 JY8QFUQ01AAWVK 7443 productive Homsap IGHV3-7*01 F 990 95.39 207/217 nt NA Homsap IGHJ4*03 F (see comment) 78 62.5 30/48 nt Homsap IGHD3-16*02 F 3 8 8 11 8.8.11 [1.17.38.11] CAKGGGNGDLDYW in-frame + Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) ctctggattcacctttagtaaccattggatgaactgggtccgccaggctccagggaaggggctggagtgggtggccaacataatgccagatggaggtgagaaattctatgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgaaggggggtggtaacggcgaccttgactactggggccaggggaacccctggtcaccgtctcctcagcatcccccgaccagcccccaaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 84 [286] g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)|g119>a,S40>N(- - -)|a170>t,K57>M(- + -)|a173>c,Q58>P(- - -)|a187>g,S63>G(+ + -)|a197>t,Y66>F(- + -)|a225>g|a245>g,N82>S(- - -)|c265>t| g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)| g119>a,S40>N(- - -)| a170>t,K57>M(- + -)|a173>c,Q58>P(- - -)|a187>g,S63>G(+ + -)| a197>t,Y66>F(- + -)|a225>g|a245>g,N82>S(- - -)|c265>t| g317>a,R106>K(+ + +)|a318>g,R106>K(+ + +)|a320>g| 316 (320) 221 (225) 211 (212) 10 (13) 2 (3) 8 (10) 3 (5) 2 (3) 1 1 1 0 2 0 0 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 0 0 1 0 1 0 0 0 0 0 117 114 110 4 2 2 2 0 1 0 0 0 1 0 0 0 0 0 4 (8) 4 4 (5) 0 (3) 0 (1) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtaaccattgg atgaactgggtccgccaggctccagggaaggggctggagtgggtggccaac ataatgccagatggaggtgagaaa ttctatgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgaaggggggtggtaacggcgaccttgactac AKGGGNGDLDY IGHV3-7 IGHD3-16 IGHJ4 ggattcacctttagtaaccattgg atgaactgggtccgccaggctccagggaaggggctggagtgggtggccaac ataatgccagatggaggtgagaaa ttctatgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgaaggggggtggtaacggcgaccttgactac IGA1 10 213 7 3 3 3 4 7 2 0 3 5 JY8QFUQ01AAZ6M 14388 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*02 F or Homsap IGHV4-39*05 F 992 94.57 209/221 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-6*01 F 3 10 7 12 10.7.12 [1.17.38.11] CARHSRLVRYFEYW in-frame + tctctggtgactccatcagcagtagttcttactactggggctggatccgccagcccccagggaaggggctggagtggattgggagtatctttcatagagggagcacctactccaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccttttctccctgaatctgagctctgtgaccgccacagacacggctgtttattactgtgcgagacattcgagactcgtccgttactttgaatactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [288] g83>a,G28>D(- - -)|a106>t|g107>c|a170>t,Y57>F(- + -)|t172>c,Y58>H(+ - -)|t177>a,S59>R(- - -)|a200>c,Y67>S(+ - -)|a257>t,Q86>L(- - -)|g258>t,Q86>L(- - -)|g270>t,K90>N(+ - -)|g289>a,A97>T(- - -)|g303>t| g83>a,G28>D(- - -)|a106>t|g107>c| a170>t,Y57>F(- + -)|t172>c,Y58>H(+ - -)|t177>a,S59>R(- - -)| a200>c,Y67>S(+ - -)|a257>t,Q86>L(- - -)|g258>t,Q86>L(- - -)|g270>t,K90>N(+ - -)|g289>a,A97>T(- - -)|g303>t| 320 229 217 12 3 9 0 2 0 1 1 0 3 1 1 0 3 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 2 1 0 1 0 0 0 0 1 0 1 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 18 3 0 3 0 0 0 1 0 0 1 1 0 0 0 0 117 114 108 6 1 5 0 1 0 0 1 0 1 0 0 0 3 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatcagcagtagttcttactac tggggctggatccgccagcccccagggaaggggctggagtggattgggagt atctttcatagagggagcacc tactccaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccttttctccctgaatctgagctctgtgaccgccacagacacggctgtttattactgt gcgagacattcgagactcgtccgttactttgaatac ARHSRLVRYFEY IGHV4-39 IGHD6-6 IGHJ4 ggtgactccatcagcagtagttcttactac tggggctggatccgccagcccccagggaaggggctggagtggattgggagt atctttcatagagggagcacc tactccaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccttttctccctgaatctgagctctgtgaccgccacagacacggctgtttattactgt gcgagacattcgagactcgtccgttactttgaatac IGA1 12 216 3 9 2 6 1 6 1 2 5 4 JY8QFUQ01AB19P 5385 productive Homsap IGHV3-7*01 F 846 88.02 191/217 nt NA Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F (see comment) 159 81.25 39/48 nt Homsap IGHD6-19*01 F 1 8 8 12 8.8.12 [1.17.38.11] CVRDRPGGWALDYW in-frame + Other possibilities: Homsap_IGHJ1*01 (highest number of consecutive identical nucleotides) ctctggattcagttttagtgacttttggatgcactgggtccgccaggctccagggaaggggctggagtgggtggcccacgtgaaccaagacgggactgagagatactatgccgactctgtgaagggccgcttcaccatctccagagacaacgccaagaaatcactgtttctgcaaatgaatggcctgagagccgaggacacggctctttattggtgtgtgagagatcgtccgggtggctgggcccttgattactggggccagggcaccctggccgccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactca NA IGG1 100 100 [285] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>t,Y37>F(- + -)|a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)|a163>c,N55>H(- - -)|a166>g,I56>V(+ - +)|a168>g,I56>V(+ - +)|g171>c,K57>N(+ - -)|t177>c|a186>g|g188>c,S63>T(+ - +)|a194>g,K65>R(+ + +)|t203>c,V68>A(+ - +)|g204>c,V68>A(+ - +)|a225>c|c255>a,N85>K(+ - -)|a263>t,Y88>F(- + -)|c276>t|a277>g,S93>G(+ + -)|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)|a308>g,Y103>W(- + -)|c309>g,Y103>W(- + -)|c314>t,A105>V(+ - +)| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>t,Y37>F(- + -)| a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)|a163>c,N55>H(- - -)| a166>g,I56>V(+ - +)|a168>g,I56>V(+ - +)|g171>c,K57>N(+ - -)|t177>c|a186>g|g188>c,S63>T(+ - +)|a194>g,K65>R(+ + +)| t203>c,V68>A(+ - +)|g204>c,V68>A(+ - +)|a225>c|c255>a,N85>K(+ - -)|a263>t,Y88>F(- + -)|c276>t|a277>g,S93>G(+ + -)|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)|a308>g,Y103>W(- + -)|c309>g,Y103>W(- + -)| c314>t,A105>V(+ - +)| 320 225 198 27 4 23 7 2 3 2 3 1 2 0 4 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 1 1 0 0 0 1 0 0 1 0 0 51 51 48 3 0 3 0 1 0 0 2 0 0 0 0 0 0 0 30 24 17 7 2 5 4 0 0 1 0 0 0 0 2 0 0 0 117 114 103 11 2 9 2 0 1 1 1 1 1 0 2 1 1 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagttttagtgacttttgg atgcactgggtccgccaggctccagggaaggggctggagtgggtggcccac gtgaaccaagacgggactgagaga tactatgccgactctgtgaagggccgcttcaccatctccagagacaacgccaagaaatcactgtttctgcaaatgaatggcctgagagccgaggacacggctctttattggtgt gtgagagatcgtccgggtggctgggcccttgattac VRDRPGGWALDY IGHV3-7 IGHD6-19 IGHJ4 ggattcagttttagtgacttttgg atgcactgggtccgccaggctccagggaaggggctggagtgggtggcccac gtgaaccaagacgggactgagaga tactatgccgactctgtgaagggccgcttcaccatctccagagacaacgccaagaaatcactgtttctgcaaatgaatggcctgagagccgaggacacggctctttattggtgt gtgagagatcgtccgggtggctgggcccttgattac IGG1 26 213 13 13 4 12 9 14 2 2 12 10 JY8QFUQ01AB1K5 18793 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD5-24*01 ORF 1 8 8 12 8.8.12 [1.17.38.11] CARDRLTISALDYW in-frame + ctctggattcacctttaacaactacgccatgtcctgggtccgccaggctccagggaaggggcttgagtgggtctcagctataactgatagcggtctttacacatactacgcagactccgtgaggggccggttcaccgtctccagagacacttccaagaacacgctgtttctgcaaatggacagcctgagagccgaggacacggccgtatatttctgtgcgagggatagactcacgatttcagcccttgattactggggccagggagccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaac NA IGG2 100 100 [285] g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|t111>c|a118>t|g119>c|g150>t|t168>a|g170>c,S57>T(+ - +)|g173>a,G58>D(- - -)|t177>c|g187>c,G63>L(- - -)|g188>t,G63>L(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|a245>c,N82>T(- + -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|a308>t,Y103>F(- + -)| g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|t111>c| a118>t|g119>c|g150>t| t168>a|g170>c,S57>T(+ - +)|g173>a,G58>D(- - -)|t177>c|g187>c,G63>L(- - -)|g188>t,G63>L(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)| a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|a245>c,N82>T(- + -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|a308>t,Y103>F(- + -)| a317>g,K106>R(+ + +)|a318>g,K106>R(+ + +)| 316 (320) 221 (225) 201 (203) 20 (22) 6 14 (16) 3 (5) 4 0 2 1 0 4 1 3 0 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 2 0 1 0 0 0 0 0 0 0 0 51 51 48 3 3 0 0 0 0 0 0 0 1 0 1 0 1 0 30 24 16 8 2 6 0 2 0 1 0 0 1 1 2 0 1 0 117 114 108 6 0 6 3 0 0 0 1 0 2 0 0 0 0 0 4 (8) 4 4 (6) 0 (2) 0 0 (2) 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaacaactacgcc atgtcctgggtccgccaggctccagggaaggggcttgagtgggtctcagct ataactgatagcggtctttacaca tactacgcagactccgtgaggggccggttcaccgtctccagagacacttccaagaacacgctgtttctgcaaatggacagcctgagagccgaggacacggccgtatatttctgt gcgagggatagactcacgatttcagcccttgattac ARDRLTISALDY IGHV3-23 IGHD5-24 IGHJ4 ggattcacctttaacaactacgcc atgtcctgggtccgccaggctccagggaaggggcttgagtgggtctcagct ataactgatagcggtctttacaca tactacgcagactccgtgaggggccggttcaccgtctccagagacacttccaagaacacgctgtttctgcaaatggacagcctgagagccgaggacacggccgtatatttctgt gcgagggatagactcacgatttcagcccttgattac IGG2 20 213 9 11 4 9 5 11 3 3 6 8 JY8QFUQ01AB3G5 11938 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 942 92.27 203/220 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 3 10 7 19 10.7.19 [1.17.38.11] CARQSFDFAWQWQVDYYFEYW in-frame + ctctggtggctccgtcagcagtagcacttactactggggctggctccgccagtccccagggaaggctctggagtggattgggactatccatcatagtgggagcacctaccagaacccgtccctcaagagtcgagtcaccatgtccgtagacacgtccaggaaccagttctccctgaggctgagctctgtgaccgccgcagacacggctctttattactgtgcgagacaaagctttgatttcgcgtggcagtggcaggtagattattactttgaatactggggcctgggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtcggtcatcgcctgcctggtccagggcttcttcc NA IGA1 100 95 [308, 309] a88>g,I30>V(+ - +)|t105>c|g107>c,S36>T(+ - +)|a124>c,I42>L(+ + +)|c133>t,P45>S(+ - -)|g146>c,G49>A(- + -)|g147>t,G49>A(- + -)|g164>c,S55>T(+ - +)|t169>c,Y57>H(+ - -)|t172>c,Y58>H(+ - -)|t199>c,Y67>Q(- - -)|c201>g,Y67>Q(- - -)|a234>g,I78>M(+ + -)|a251>g,K84>R(+ + +)|a269>g,K90>R(+ + +)|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)| a88>g,I30>V(+ - +)|t105>c|g107>c,S36>T(+ - +)| a124>c,I42>L(+ + +)|c133>t,P45>S(+ - -)|g146>c,G49>A(- + -)|g147>t,G49>A(- + -)|g164>c,S55>T(+ - +)| t169>c,Y57>H(+ - -)|t172>c,Y58>H(+ - -)| t199>c,Y67>Q(- - -)|c201>g,Y67>Q(- - -)|a234>g,I78>M(+ + -)|a251>g,K84>R(+ + +)|a269>g,K90>R(+ + +)|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)| 320 228 211 17 1 16 4 0 1 4 1 0 0 0 4 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 1 2 1 0 0 1 0 0 0 0 1 0 0 0 51 51 46 5 0 5 0 0 1 0 1 0 0 0 2 0 1 0 30 21 19 2 0 2 0 0 0 2 0 0 0 0 0 0 0 0 117 114 107 7 0 7 3 0 0 1 0 0 0 0 1 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtggctccgtcagcagtagcacttactac tggggctggctccgccagtccccagggaaggctctggagtggattgggact atccatcatagtgggagcacc taccagaacccgtccctcaagagtcgagtcaccatgtccgtagacacgtccaggaaccagttctccctgaggctgagctctgtgaccgccgcagacacggctctttattactgt gcgagacaaagctttgatttcgcgtggcagtggcaggtagattattactttgaatac ARQSFDFAWQWQVDYYFEY IGHV4-39 IGHD6-19 IGHJ4 ggtggctccgtcagcagtagcacttactac tggggctggctccgccagtccccagggaaggctctggagtggattgggact atccatcatagtgggagcacc taccagaacccgtccctcaagagtcgagtcaccatgtccgtagacacgtccaggaaccagttctccctgaggctgagctctgtgaccgccgcagacacggctctttattactgt gcgagacaaagctttgatttcgcgtggcagtggcaggtagattattactttgaatac IGA1 17 216 9 8 1 8 8 9 0 1 12 4 JY8QFUQ01AB3KI 17371 productive (see comment) Homsap IGHV3-11*01 F 862 89.67 191/213 nt 89.2 190/213 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-2*01 F 2 8 7 14 8.7.14 [1.17.38.11] CARDPYCSSTNCYPYW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 63 of V-REGION: 3 nucleotides (from position 94 in the user submitted sequence), (do not cause frameshift) tctggattcaccctcagtgactactacatgagttggttccgccaggctccagggaaggggctggagtggctttcatacattgcaggaagtggaaccacatattacgcagattctgtgaagggccgattcaccatctccagggacaatgccgagcactcggtatacctgcaaatgaacagcctgagagtcgaagacacggccgtgtattactgtgcgagagatccctattgtagtagtaccaactgctatccctactggggccagggaacccaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [286] t88>c,F30>L(+ - -)|c120>t|a124>t,I42>F(+ - -)|g157>c,V53>L(+ - +)|a169>g,S57>A(- + -)|g170>c,S57>A(- + -)|t171>a,S57>A(- + -)|a172>g,S58>G(+ + -)|t174>a,S58>G(+ + -)|t186>a|t194>c,I65>T(- - -)|c198>t|c207>t|c246>t|a250>g,K84>E(+ - -)|a253>c,N85>H(- - -)|a258>g|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t264>c|c287>t,A96>V(+ - +)|g291>a| t88>c,F30>L(+ - -)| c120>t|a124>t,I42>F(+ - -)|g157>c,V53>L(+ - +)| a169>g,S57>A(- + -)|g170>c,S57>A(- + -)|t171>a,S57>A(- + -)|a172>g,S58>G(+ + -)|t174>a,S58>G(+ + -)|t186>a|t194>c,I65>T(- - -)| c198>t|c207>t|c246>t|a250>g,K84>E(+ - -)|a253>c,N85>H(- - -)|a258>g|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t264>c|c287>t,A96>V(+ - +)|g291>a| 320 221 199 22 8 14 4 2 5 3 1 0 1 3 2 1 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 51 51 48 3 1 2 0 0 1 0 0 0 1 0 1 0 0 0 30 21 14 7 1 6 2 0 0 1 0 0 0 3 1 0 0 0 117 114 103 11 6 5 2 2 4 1 1 0 0 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccctcagtgactactac atgagttggttccgccaggctccagggaaggggctggagtggctttcatac attgcaggaagtggaaccaca tattacgcagattctgtgaagggccgattcaccatctccagggacaatgccgagcactcggtatacctgcaaatgaacagcctgagagtcgaagacacggccgtgtattactgt gcgagagatccctattgtagtagtaccaactgctatccctac ARDPYCSSTNCYPY IGHV3-11 IGHD2-2 IGHJ4 ggattcaccctcagtgactactac atgagttggttccgccaggctccagggaaggggctggagtggctttcatac attgcaggaagtggaaccaca tattacgcagattctgtgaagggccgattcaccatctccagggacaatgccgagcactcggtatacctgcaaatgaacagcctgagagtcgaagacacggccgtgtattactgt gcgagagatccctattgtagtagtaccaactgctatccctac IGA1 22 210 14 8 7 10 7 12 7 1 7 7 JY8QFUQ01AB51E 7005 productive Homsap IGHV3-48*02 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-16*01 F 3 8 8 13 8.8.13 [1.17.38.11] CARDMIIFGGALDNW in-frame - ctctggattcgccttcagtagttccagcatgaactgggtccgccagggtccagggaaggggctggagtggatttcacacattaggggtagtagtagtaccacccactacgcagactctgtgaagggccggttcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagacgaggacacggctgtctattactgtgcgagagatatgattatctttgggggagcccttgacaactggggccagggaaccctggtcaccgtctcctctgcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] a85>g,T29>A(- - -)|c108>t|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)|c134>g,A45>G(- + -)|g157>a,V53>I(+ - +)|t163>c,Y55>H(+ - -)|t171>g,S57>R(- - -)|a172>g,S58>G(+ + -)|t194>c,I65>T(- - -)|a195>c,I65>T(- - -)|t196>c,Y66>H(+ - -)|a225>g|g303>c| a85>g,T29>A(- - -)|c108>t|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)| c134>g,A45>G(- + -)|g157>a,V53>I(+ - +)|t163>c,Y55>H(+ - -)| t171>g,S57>R(- - -)|a172>g,S58>G(+ + -)|t194>c,I65>T(- - -)|a195>c,I65>T(- - -)| t196>c,Y66>H(+ - -)|a225>g|g303>c| 320 225 211 14 3 11 3 1 1 4 2 0 0 0 1 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 1 1 1 0 0 0 0 0 0 0 51 51 48 3 0 3 0 1 0 1 0 0 0 0 0 1 0 0 30 24 20 4 0 4 1 0 0 1 1 0 0 0 0 0 0 1 117 114 111 3 2 1 1 0 0 1 0 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ctct ggattcgccttcagtagttccagc atgaactgggtccgccagggtccagggaaggggctggagtggatttcacac attaggggtagtagtagtaccacc cactacgcagactctgtgaagggccggttcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagacgaggacacggctgtctattactgt gcgagagatatgattatctttgggggagcccttgacaac ARDMIIFGGALDN IGHV3-48 IGHD3-16 IGHJ4 ggattcgccttcagtagttccagc atgaactgggtccgccagggtccagggaaggggctggagtggatttcacac attaggggtagtagtagtaccacc cactacgcagactctgtgaagggccggttcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagacgaggacacggctgtctattactgt gcgagagatatgattatctttgggggagcccttgacaac IGA1 14 213 9 5 2 4 7 10 2 1 4 7 JY8QFUQ01ABDY1 10228 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 990 95.39 207/217 nt NA Homsap IGHJ3*02 F 232 96 48/50 nt Homsap IGHD2-21*02 F 2 8 8 15 8.8.15 [1.17.38.11] CARLCGNDCRNDVFDIW in-frame + ctctggattcaccttcagtacctactggatgcactgggtccgccaagttccagggaaggggctggtgtgggtctcacgtgttgatagtgatgggactagcacagtctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctatatctgcaaatgaacagtctgaaagccgaggacacggctgtatattactgtgcaagattatgtggtaatgactgccgcaatgatgtttttgatatctggggccaagggacattggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 100 100 [293] g107>c,S36>T(+ - +)|c134>t,A45>V(+ - +)|a166>g,I56>V(+ - +)|a169>g,N57>D(+ + -)|g188>c,S63>T(+ - +)|a196>g,S66>V(- - -)|g197>t,S66>V(- - -)|g261>a|g284>a,R95>K(+ + +)|g303>a| g107>c,S36>T(+ - +)| c134>t,A45>V(+ - +)| a166>g,I56>V(+ - +)|a169>g,N57>D(+ + -)|g188>c,S63>T(+ - +)| a196>g,S66>V(- - -)|g197>t,S66>V(- - -)|g261>a|g284>a,R95>K(+ + +)|g303>a| g319>t|a320>t| 318 (320) 223 (225) 213 10 (12) 2 (4) 8 3 3 1 0 0 0 0 (1) 0 2 0 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 2 0 0 0 0 0 0 0 1 0 0 0 117 114 109 5 2 3 1 3 0 0 0 0 0 0 0 0 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtacctactgg atgcactgggtccgccaagttccagggaaggggctggtgtgggtctcacgt gttgatagtgatgggactagcaca gtctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctatatctgcaaatgaacagtctgaaagccgaggacacggctgtatattactgt gcaagattatgtggtaatgactgccgcaatgatgtttttgatatc ARLCGNDCRNDVFDI IGHV3-74 IGHD2-21 IGHJ3 ggattcaccttcagtacctactgg atgcactgggtccgccaagttccagggaaggggctggtgtgggtctcacgt gttgatagtgatgggactagcaca gtctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctatatctgcaaatgaacagtctgaaagccgaggacacggctgtatattactgt gcaagattatgtggtaatgactgccgcaatgatgtttttgatatc IGA2 10 213 7 3 4 7 3 3 2 0 4 4 JY8QFUQ01ABF8G 6209 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 875 88.69 196/221 nt NA Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD6-6*01 F 2 10 7 16 10.7.16 [1.17.38.11] CTNDRSMAVRATHAFHIW in-frame + tctctggtgactccattggtagcagtgcctactactggggctgggtccgccagccccccgggaaggggctggagtggattggaagtatctattatggtggcaacacctactacaacccgtccctcaggagtcgagtcagcatttccgcagacacgtccaagaaccagttctccctgcatctctactccgtgaccgccgcagacacggctctgtattactgtacgaatgaccgttcgatggccgttcgtgcaactcatgcttttcatatctggggcctagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [300] g83>a,G28>D(- - -)|c90>t|a91>g,S31>G(+ + -)|c93>t,S31>G(+ + -)|t102>c|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|t108>c,S36>A(- + -)|a124>g,I42>V(+ - +)|a138>c|g162>a|a175>g,S59>G(+ + -)|g189>c|g191>a,S64>N(- - -)|a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|a234>t|t239>c,V80>A(+ - +)|a268>c,K90>H(- - +)|g270>t,K90>H(- - +)|g273>c|a274>t,S92>Y(+ - -)|g275>a,S92>Y(+ - -)|t279>c|g301>c,V101>L(+ - +)|g313>a,A105>T(- - -)| g83>a,G28>D(- - -)|c90>t|a91>g,S31>G(+ + -)|c93>t,S31>G(+ + -)|t102>c|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|t108>c,S36>A(- + -)| a124>g,I42>V(+ - +)|a138>c|g162>a| a175>g,S59>G(+ + -)|g189>c|g191>a,S64>N(- - -)| a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|a234>t|t239>c,V80>A(+ - +)|a268>c,K90>H(- - +)|g270>t,K90>H(- - +)|g273>c|a274>t,S92>Y(+ - -)|g275>a,S92>Y(+ - -)|t279>c|g301>c,V101>L(+ - +)| g313>a,A105>T(- - -)|g317>a,R106>N(+ - -)|a318>t,R106>N(+ - -)|c319>g| 316 (320) 225 (229) 199 (200) 26 (29) 8 (9) 18 (20) 5 5 (6) 2 4 2 0 2 (3) 0 4 1 (2) 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 22 8 2 6 2 1 2 2 0 0 0 0 1 0 0 0 51 51 48 3 2 1 1 1 0 0 1 0 0 0 0 0 0 0 30 21 18 3 1 2 1 1 0 0 0 0 0 0 1 0 0 0 117 114 103 11 3 8 1 1 0 2 1 0 2 0 2 1 1 0 4 (8) 4 3 (4) 1 (4) 0 (1) 1 (3) 0 1 (2) 0 0 0 0 0 (1) 0 0 0 (1) 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccattggtagcagtgcctactac tggggctgggtccgccagccccccgggaaggggctggagtggattggaagt atctattatggtggcaacacc tactacaacccgtccctcaggagtcgagtcagcatttccgcagacacgtccaagaaccagttctccctgcatctctactccgtgaccgccgcagacacggctctgtattactgt acgaatgaccgttcgatggccgttcgtgcaactcatgcttttcatatc TNDRSMAVRATHAFHI IGHV4-39 IGHD6-6 IGHJ3 ggtgactccattggtagcagtgcctactac tggggctgggtccgccagccccccgggaaggggctggagtggattggaagt atctattatggtggcaacacc tactacaacccgtccctcaggagtcgagtcagcatttccgcagacacgtccaagaaccagttctccctgcatctctactccgtgaccgccgcagacacggctctgtattactgt acgaatgaccgttcgatggccgttcgtgcaactcatgcttttcatatc IGA1 25 216 15 10 6 12 9 13 5 3 9 8 JY8QFUQ01ABFPI 11583 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 945 93.09 202/217 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD5-12*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARGPRSRGYGMDVW in-frame - ctctggattcaccttcaacaactatgccatgagttgggtccgccaggctccagggaaggggctggaatgggtctcaactattactagtggtggtggtagtacattgtacgcagactccgtgaagggccggttcaccatctccagagacaatttcaaggacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgagagggcctcgatcgcgcggctacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccttgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [288] t90>c|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|c120>t|g153>a|g163>a,A55>T(- - -)|g170>c,S57>T(+ - +)|g172>a,G58>S(+ + -)|a175>g,S59>G(+ + -)|c192>t|a197>t,Y66>L(- - -)|c198>g,Y66>L(- - -)|c248>t,S83>F(- - -)|a253>g,N85>D(+ + -)|g261>a|a317>g,K106>R(+ + +)| t90>c|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)| c120>t|g153>a|g163>a,A55>T(- - -)| g170>c,S57>T(+ - +)|g172>a,G58>S(+ + -)|a175>g,S59>G(+ + -)|c192>t| a197>t,Y66>L(- - -)|c198>g,Y66>L(- - -)|c248>t,S83>F(- - -)|a253>g,N85>D(+ + -)|g261>a| a317>g,K106>R(+ + +)|a320>g| 319 (320) 224 (225) 208 16 (17) 5 (6) 11 3 (4) 6 3 1 0 0 1 0 1 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 2 0 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 2 1 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 1 1 1 0 0 0 0 0 1 0 0 0 117 114 109 5 1 4 1 1 1 0 0 0 1 0 0 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 1 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcaacaactatgcc atgagttgggtccgccaggctccagggaaggggctggaatgggtctcaact attactagtggtggtggtagtaca ttgtacgcagactccgtgaagggccggttcaccatctccagagacaatttcaaggacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagagggcctcgatcgcgcggctacggtatggacgtc ARGPRSRGYGMDV IGHV3-23 IGHD5-12 IGHJ6 ggattcaccttcaacaactatgcc atgagttgggtccgccaggctccagggaaggggctggaatgggtctcaact attactagtggtggtggtagtaca ttgtacgcagactccgtgaagggccggttcaccatctccagagacaatttcaaggacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagagggcctcgatcgcgcggctacggtatggacgtc IGG1 15 213 12 3 9 11 3 4 3 2 5 5 JY8QFUQ01ABGCX 18224 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 959 93.58 204/218 nt NA Homsap IGHJ4*02 F (see comment) 177 85.42 41/48 nt Homsap IGHD5-12*01 F 1 8 8 9 8.8.9 [1.17.38.11] CARLPNTIDSW in-frame + Other possibilities: Homsap_IGHJ5*01 (highest number of consecutive identical nucleotides) cttctggatatagttttgccacctactggatcggctgggtgcgccagaggcccgggaagggcctggagtggatgggggtcatctatcctggtgactctgataccagatacagcccgtccttccaaggccaggtcaccatttcagccgacaagtccctcagtatcgcctacctgcagtggagcagcctgcaggcctcggacaccgccatatattactgtgcgagactgcctaacactattgactcctggggccaggggaccctggtcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [276] g74>c|c84>t|c87>t|a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)|t134>g,M45>R(- + -)|a144>g|a163>g,I55>V(+ - +)|c234>t|a250>c,I84>L(+ + +)|c255>t|c257>t,T86>I(- - -)|a283>c,K95>Q(+ - -)|g303>a,M101>I(+ + -)| g74>c| c84>t|c87>t|a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)| t134>g,M45>R(- + -)|a144>g|a163>g,I55>V(+ - +)| c234>t|a250>c,I84>L(+ + +)|c255>t|c257>t,T86>I(- - -)|a283>c,K95>Q(+ - -)|g303>a,M101>I(+ + -)| a320>t| 319 (320) 225 (226) 211 14 (15) 6 (7) 8 3 1 5 0 2 0 0 (1) 0 2 0 0 1 78 5 4 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 36 24 20 4 2 2 1 0 2 0 0 0 0 0 1 0 0 0 51 51 48 3 1 2 2 0 0 0 0 0 0 0 0 0 0 1 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 2 4 0 1 3 0 2 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| cttct ggatatagttttgccacctactgg atcggctgggtgcgccagaggcccgggaagggcctggagtggatgggggtc atctatcctggtgactctgatacc agatacagcccgtccttccaaggccaggtcaccatttcagccgacaagtccctcagtatcgcctacctgcagtggagcagcctgcaggcctcggacaccgccatatattactgt gcgagactgcctaacactattgactcc ARLPNTIDS IGHV5-51 IGHD5-12 IGHJ4 ggatatagttttgccacctactgg atcggctgggtgcgccagaggcccgggaagggcctggagtggatgggggtc atctatcctggtgactctgatacc agatacagcccgtccttccaaggccaggtcaccatttcagccgacaagtccctcagtatcgcctacctgcagtggagcagcctgcaggcctcggacaccgccatatattactgt gcgagactgcctaacactattgactcc IGA2 13 213 9 4 6 7 3 6 3 2 6 2 JY8QFUQ01ABI4U 11617 productive (see comment) Homsap IGHV4-39*03 F 928 91.74 200/218 nt 91.28 199/218 nt Homsap IGHJ3*01 F 232 96 48/50 nt Homsap IGHD3-22*01 F 3 9 7 17 9.7.17 [1.17.38.11] CARSITLTVVVMNDAFDVW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 35 of V-REGION: 1 nucleotides (from position 25 in the user submitted sequence), (cause frameshift) ctccggtggctccatcaacagtagaattattattggggctggatccgccagcccccagggaagggtttggagtggattggaaatatctattatagtgggaacacctactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgtgcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtctggggccaagggacattggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [302] t78>c|g92>a,S31>N(- - -)|g107>a,S36>N(- - -)|c111>t|c114>t|g147>t|c148>t|g162>a|g164>a,S55>N(- - -)|g191>a,S64>N(- - -)|c204>t|a232>g,I78>V(+ - +)|c245>g,T82>R(- - -)|c249>t|c261>g,F87>L(+ - -)|g275>c,S92>T(+ - +)|g280>c,V94>L(+ - +)|g303>a| t78>c| g92>a,S31>N(- - -)|g107>a,S36>N(- - -)|c111>t|c114>t| g147>t|c148>t|g162>a|g164>a,S55>N(- - -)| g191>a,S64>N(- - -)| c204>t|a232>g,I78>V(+ - +)|c245>g,T82>R(- - -)|c249>t|c261>g,F87>L(+ - -)|g275>c,S92>T(+ - +)|g280>c,V94>L(+ - +)|g303>a| 311 218 200 18 9 9 1 6 5 1 0 0 0 0 2 2 1 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 29 25 4 2 2 0 2 2 0 0 0 0 0 0 0 0 0 51 51 47 4 3 1 0 2 1 0 0 0 0 0 0 0 1 0 30 21 20 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 116 114 105 8 3 5 1 1 2 0 0 0 0 0 2 2 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctcc ggtggctccatcaacagtagaattattat tggggctggatccgccagcccccagggaagggtttggagtggattggaaat atctattatagtgggaacacc tactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgt gcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtc ARSITLTVVVMNDAFDV IGHV4-39 IGHD3-22 IGHJ3 ggtggctccatcaacagtagaattattat tggggctggatccgccagcccccagggaagggtttggagtggattggaaat atctattatagtgggaacacc tactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgt gcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtc IGG1 17 215 12 5 11 16 1 1 6 2 6 3 JY8QFUQ01ABKJ7 20800 productive Homsap IGHV3-11*01 F 883 89.5 196/219 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-15*01 F 2 8 8 14 8.8.14 [2.17.38.11] CARDDSCSGGSCYEYW in-frame - acctctggattcagtttcagtgactactacatgagttggatccgccaggctccagggagggggctggagtgggtttcatatattgatagcggtggtaccgccatgtactactcagactccgtgaagggccgattcaccatctccagggacaacgccaagaagtcactgtttctgcaaatgagtagcctgagagccgaagacacggccgtgtactattgtgcgagagatgactcttgtagtggtggtagctgttacgaatactggggccagggaaccccggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [292] g73>a,A25>T(- - -)|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c120>t|a143>g,K48>R(+ + +)|c165>t|a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|t174>c|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)|a190>g,T64>A(- - -)|a195>g,I65>M(+ + -)|g202>t,A68>S(- + -)|t210>c|c255>g,N85>K(+ - -)|a263>t,Y88>F(- + -)|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|g291>a|t306>c|c309>t| g73>a,A25>T(- - -)| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)| c120>t|a143>g,K48>R(+ + +)|c165>t| a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|t174>c|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)|a190>g,T64>A(- - -)|a195>g,I65>M(+ + -)| g202>t,A68>S(- + -)|t210>c|c255>g,N85>K(+ - -)|a263>t,Y88>F(- + -)|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|g291>a|t306>c|c309>t| 320 227 204 23 7 16 6 3 5 4 0 0 1 0 1 2 1 0 78 6 5 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 1 0 0 0 0 0 0 1 0 0 51 51 48 3 2 1 1 0 2 0 0 0 0 0 0 0 0 0 30 24 16 8 1 7 4 1 0 2 0 0 0 0 1 0 0 0 117 114 105 9 4 5 1 1 2 2 0 0 1 0 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| acctct ggattcagtttcagtgactactac atgagttggatccgccaggctccagggagggggctggagtgggtttcatat attgatagcggtggtaccgccatg tactactcagactccgtgaagggccgattcaccatctccagggacaacgccaagaagtcactgtttctgcaaatgagtagcctgagagccgaagacacggccgtgtactattgt gcgagagatgactcttgtagtggtggtagctgttacgaatac ARDDSCSGGSCYEY IGHV3-11 IGHD2-15 IGHJ4 ggattcagtttcagtgactactac atgagttggatccgccaggctccagggagggggctggagtgggtttcatat attgatagcggtggtaccgccatg tactactcagactccgtgaagggccgattcaccatctccagggacaacgccaagaagtcactgtttctgcaaatgagtagcctgagagccgaagacacggccgtgtactattgt gcgagagatgactcttgtagtggtggtagctgttacgaatac IGA1 22 213 17 5 7 11 10 11 6 1 6 9 JY8QFUQ01ABL5K 5031 productive Homsap IGHV3-30*07 F, or Homsap IGHV3-30*16 F 918 91.71 199/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 2 8 8 17 8.8.17 [1.17.38.11] CARDSVPRASEAAYYFDSW in-frame + ctctggattcaccttcagtatttattctatgcattgggtccgccaggccccaggcaaggggctagagtgggtggcagttatatcacatgatggaactaataaatattactcagactccgtgaagggccgattcaccatctccagagacaattccaagagcgcgctgtatctgcagctgaacgtcctgagcgccgaggacacggctgtctatttctgtgcgagagattccgtacctcgggcgtcagaggctgcctactactttgactcttggggccagggaaccctggtcaccgtctccccagcatccccgaccagcccaaggtcttcccgctgacgcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 86 84 [299, 298] g107>t,S36>I(- - -)|c108>t,S36>I(- - -)|g112>t,A38>S(- + -)|c120>t|t135>c|t172>c,Y58>H(+ - -)|g188>c,S63>T(+ - +)|c198>t|g202>t,A68>S(- + -)|a254>g,N85>S(- - -)|a256>g,T86>A(- - -)|a270>g|a271>c,M91>L(+ + -)|a277>g,S93>V(- - -)|g278>t,S93>V(- - -)|a285>c,R95>S(- - -)|g303>c|a308>t,Y103>F(- + -)| g107>t,S36>I(- - -)|c108>t,S36>I(- - -)|g112>t,A38>S(- + -)| c120>t|t135>c| t172>c,Y58>H(+ - -)|g188>c,S63>T(+ - +)| c198>t|g202>t,A68>S(- + -)|a254>g,N85>S(- - -)|a256>g,T86>A(- - -)|a270>g|a271>c,M91>L(+ + -)|a277>g,S93>V(- - -)|g278>t,S93>V(- - -)|a285>c,R95>S(- - -)|g303>c|a308>t,Y103>F(- + -)| 320 225 207 18 5 13 4 0 3 2 2 0 1 0 2 0 4 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 1 0 0 0 0 0 0 0 2 0 51 51 49 2 2 0 0 0 1 1 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 1 0 0 0 0 1 0 0 0 117 114 103 11 3 8 4 0 1 0 2 0 1 0 1 0 2 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtatttattct atgcattgggtccgccaggccccaggcaaggggctagagtgggtggcagtt atatcacatgatggaactaataaa tattactcagactccgtgaagggccgattcaccatctccagagacaattccaagagcgcgctgtatctgcagctgaacgtcctgagcgccgaggacacggctgtctatttctgt gcgagagattccgtacctcgggcgtcagaggctgcctactactttgactct ARDSVPRASEAAYYFDS IGHV3-30 IGHD6-19 IGHJ4 ggattcaccttcagtatttattct atgcattgggtccgccaggccccaggcaaggggctagagtgggtggcagtt atatcacatgatggaactaataaa tattactcagactccgtgaagggccgattcaccatctccagagacaattccaagagcgcgctgtatctgcagctgaacgtcctgagcgccgaggacacggctgtctatttctgt gcgagagattccgtacctcgggcgtcagaggctgcctactactttgactct IGA1 18 213 9 9 3 9 6 9 5 0 8 5 JY8QFUQ01ABTVQ 19183 productive Homsap IGHV1-18*01 F 822 87.38 187/214 nt NA Homsap IGHJ6*02 F (see comment) 166 74.19 46/62 nt Homsap IGHD2-21*02 F 3 8 8 17 8.8.17 [0.17.38.11] CVREESIVVVTGRRGMDVW in-frame - Other possibilities: Homsap_IGHJ4*03 (shorter alignment but highest percentage of identity) cggttacattgttaccacctctggtttcagctgggtgcgacaggcccctggacaaggcctggagtggatgggatgggtcagcggttataatgataaatccaactatgcacagaagttcaaagacagaattatcatgaccacagacatatcaacgagcacagcctacatggagctgaggagcctgagatctgacgacacggccatgtattattgtgttagagaagagtcaattgtggtggtgaccgggcggcggggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [296] t78>c|c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|t88>g,F30>V(+ - -)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|a115>t,I39>F(+ - -)|g147>c|t150>g|a166>g,I56>V(+ - +)|c173>g,A58>G(- + -)|c177>t|g188>a,G63>D(- - -)|c192>a,N64>K(+ - -)|a193>t,T65>S(+ - +)|a195>c,T65>S(+ - +)|c211>t,L71>F(+ - -)|c214>a,Q72>K(+ - -)|g216>a,Q72>K(+ - -)|g221>a,G74>D(- - -)|g226>a,V76>I(+ - +)|c228>t,V76>I(+ - +)|c230>t,T77>I(- - -)|c245>t,T82>I(- - -)|c249>a|g301>a,V101>M(+ - -)|c309>t|c314>t,A105>V(+ - +)|g315>t,A105>V(+ - +)| t78>c| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|t88>g,F30>V(+ - -)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)| a115>t,I39>F(+ - -)|g147>c|t150>g| a166>g,I56>V(+ - +)|c173>g,A58>G(- + -)|c177>t|g188>a,G63>D(- - -)|c192>a,N64>K(+ - -)|a193>t,T65>S(+ - +)|a195>c,T65>S(+ - +)| c211>t,L71>F(+ - -)|c214>a,Q72>K(+ - -)|g216>a,Q72>K(+ - -)|g221>a,G74>D(- - -)|g226>a,V76>I(+ - +)|c228>t,V76>I(+ - +)|c230>t,T77>I(- - -)|c245>t,T82>I(- - -)|c249>a|g301>a,V101>M(+ - -)|c309>t| c314>t,A105>V(+ - +)|g315>t,A105>V(+ - +)| 320 222 193 29 6 23 1 5 9 1 2 3 2 0 2 1 1 2 78 1 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 0 2 0 1 0 0 0 1 0 0 1 51 51 48 3 2 1 0 0 0 0 0 0 1 0 1 0 0 1 30 24 17 7 1 6 1 1 1 0 1 1 1 0 0 1 0 0 117 114 103 11 2 9 0 4 5 0 0 2 0 0 0 0 0 0 8 8 6 2 0 2 0 0 1 0 0 0 0 0 0 0 1 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|ta,175-176(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,5-6(FR1)|tt,75-76(FR1)|tt,81-82(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|tt,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,3-6(FR1)|agct,8-11(FR1)|agct,24-27(FR1)|ggct,72-75(FR1)|ggtt,79-82(CDR1)|agct,106-109(CDR1)|ggta,112-115(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)| agct,8-11(FR1)|agct,24-27(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|agct,209-212(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| c ggttacattgttaccacctctggt ttcagctgggtgcgacaggcccctggacaaggcctggagtggatgggatgg gtcagcggttataatgataaatcc aactatgcacagaagttcaaagacagaattatcatgaccacagacatatcaacgagcacagcctacatggagctgaggagcctgagatctgacgacacggccatgtattattgt gttagagaagagtcaattgtggtggtgaccgggcggcggggtatggacgtc VREESIVVVTGRRGMDV IGHV1-18 IGHD2-21 IGHJ6 ggttacattgttaccacctctggt ttcagctgggtgcgacaggcccctggacaaggcctggagtggatgggatgg gtcagcggttataatgataaatcc aactatgcacagaagttcaaagacagaattatcatgaccacagacatatcaacgagcacagcctacatggagctgaggagcctgagatctgacgacacggccatgtattattgt gttagagaagagtcaattgtggtggtgaccgggcggcggggtatggacgtc IGA1 26 213 14 12 13 19 1 7 4 1 10 11 JY8QFUQ01ABYQ3 11775 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 693 80.18 174/217 nt NA Homsap IGHJ3*01 F (see comment) 160 80 40/50 nt Homsap IGHD3-22*01 F 3 8 8 14 8.8.14 [1.17.38.11] CARGLGLNAVYAFDVW in-frame + Low V-REGION identity (80,18% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ6*01 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctggattcgcttttggagattatgccatgaactgggtccgccaggccccagggaagggactggagtgggtctcaggtttgagcggtagaggcgtcagcaaatattatgcagactccgtgaggggccggttctccatttccagagacaactccgggagcgaggcagtccttcaaatgagcagcctgagagtcgaggacacggccacttactactgtgcgaggggcctagggcttaatgctgtctacgcatttgacgtctggggacaggggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [290] a85>g,T29>A(- - -)|c87>t,T29>A(- - -)|a103>g,S35>G(+ + -)|c105>a,S35>G(+ + -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|g119>a,S40>N(- - -)|t135>c|g147>a|c164>g,A55>G(- + -)|a166>t,I56>L(+ + +)|t168>g,I56>L(+ + +)|t171>c|t177>a,S59>R(- - -)|t186>c|g188>t,G63>V(- - -)|t189>c,G63>V(- - -)|c194>a,T65>K(- - -)|c198>t|c201>t|a215>g,K72>R(+ + +)|a229>t,T77>S(+ - +)|c234>t|t246>c|a250>g,K84>G(- - -)|a251>g,K84>G(- - -)|a254>g,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|c259>g,L87>A(+ - +)|t260>c,L87>A(+ - +)|g261>a,L87>A(+ - +)|t262>g,Y88>V(- - -)|a263>t,Y88>V(- - -)|t264>c,Y88>V(- - -)|g267>t|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|a303>t,V101>T(- - -)|t306>c| a85>g,T29>A(- - -)|c87>t,T29>A(- - -)|a103>g,S35>G(+ + -)|c105>a,S35>G(+ + -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)| g119>a,S40>N(- - -)|t135>c|g147>a|c164>g,A55>G(- + -)| a166>t,I56>L(+ + +)|t168>g,I56>L(+ + +)|t171>c|t177>a,S59>R(- - -)|t186>c|g188>t,G63>V(- - -)|t189>c,G63>V(- - -)|c194>a,T65>K(- - -)| c198>t|c201>t|a215>g,K72>R(+ + +)|a229>t,T77>S(+ - +)|c234>t|t246>c|a250>g,K84>G(- - -)|a251>g,K84>G(- - -)|a254>g,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|c259>g,L87>A(+ - +)|t260>c,L87>A(+ - +)|g261>a,L87>A(+ - +)|t262>g,Y88>V(- - -)|a263>t,Y88>V(- - -)|t264>c,Y88>V(- - -)|g267>t|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|a303>t,V101>T(- - -)|t306>c| a317>g,K106>R(+ + +)|a318>g,K106>R(+ + +)|a320>g| 316 (320) 221 (225) 178 (179) 43 (46) 10 (11) 33 (35) 9 (12) 5 6 9 0 3 4 1 0 2 2 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 0 7 3 1 2 0 0 1 0 0 0 0 0 0 51 51 47 4 2 2 0 2 0 1 0 0 0 0 0 1 0 0 30 24 16 8 2 6 0 0 0 3 0 1 1 1 0 0 1 1 117 114 90 24 6 18 6 2 4 5 0 1 3 0 0 1 1 1 4 (8) 4 4 (5) 0 (3) 0 (1) 0 (2) 0 (3) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcgcttttggagattatgcc atgaactgggtccgccaggccccagggaagggactggagtgggtctcaggt ttgagcggtagaggcgtcagcaaa tattatgcagactccgtgaggggccggttctccatttccagagacaactccgggagcgaggcagtccttcaaatgagcagcctgagagtcgaggacacggccacttactactgt gcgaggggcctagggcttaatgctgtctacgcatttgacgtc ARGLGLNAVYAFDV IGHV3-23 IGHD3-22 IGHJ3 ggattcgcttttggagattatgcc atgaactgggtccgccaggccccagggaagggactggagtgggtctcaggt ttgagcggtagaggcgtcagcaaa tattatgcagactccgtgaggggccggttctccatttccagagacaactccgggagcgaggcagtccttcaaatgagcagcctgagagtcgaggacacggccacttactactgt gcgaggggcctagggcttaatgctgtctacgcatttgacgtc IGA1 43 213 29 14 11 18 18 25 8 2 20 13 JY8QFUQ01ABZLF 20823 productive Homsap IGHV3-7*01 F 959 93.58 204/218 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD5-18*01 F 3 8 8 15 8.8.15 [1.17.38.11] CARVGYGYRGIYFFDYW in-frame + cctctacattcacgtttagtcggtattggatgagctgggtccgccaggctccagggaagggcctggagtgggtggccaacataaaggaagacggaagtgagagatattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgtgcgagagtgggctatggttaccgaggaatatacttctttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [295] g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|c87>g|a106>c,S36>R(- - -)|c108>g,S36>R(- - -)|g147>c|c172>g,Q58>E(+ + -)|t177>c|a194>g,K65>R(+ + +)|c198>t|c246>t|a258>t|a271>t,M91>L(+ + -)|c280>t| g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|c87>g|a106>c,S36>R(- - -)|c108>g,S36>R(- - -)| g147>c| c172>g,Q58>E(+ + -)|t177>c|a194>g,K65>R(+ + +)| c198>t|c246>t|a258>t|a271>t,M91>L(+ + -)|c280>t| a320>t| 319 (320) 225 (226) 211 14 (15) 7 (8) 7 1 1 3 1 1 0 2 (3) 0 2 3 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 0 0 1 0 0 0 1 2 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 21 3 1 2 1 0 0 1 0 0 0 0 0 1 0 0 117 114 109 5 4 1 0 0 3 0 0 0 2 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct acattcacgtttagtcggtattgg atgagctgggtccgccaggctccagggaagggcctggagtgggtggccaac ataaaggaagacggaagtgagaga tattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgt gcgagagtgggctatggttaccgaggaatatacttctttgactac ARVGYGYRGIYFFDY IGHV3-7 IGHD5-18 IGHJ4 acattcacgtttagtcggtattgg atgagctgggtccgccaggctccagggaagggcctggagtgggtggccaac ataaaggaagacggaagtgagaga tattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgt gcgagagtgggctatggttaccgaggaatatacttctttgactac IGG1 14 213 6 8 4 9 2 5 5 2 1 6 JY8QFUQ01ABZPQ 10020 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 882 89.86 195/217 nt 89.4 194/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD1-26*01 F 1 8 8 15 8.8.15 [1.16.38.11] CAKDQSCIVGPCGFDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 59 in the user submitted sequence), (cause frameshift) cctccgaattcatccttgacagttatgccatgagttgggtccgccaggccccagggaagggctggagtgggtctcggctattagtggaagtggtgcaaccacatactacgcagactccgtgaagggccggttcgccatctccagagacaattccaagaacacgctatatctacaaatgaacaacctaggggccgaggacacggccgtttattactgtgcgaaagatcagtcctgtatagtgggaccttgtggttttgactactggggccagggagccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [294] t78>c|g80>a,G27>E(- - -)|c86>t,T29>I(- - -)|t88>c,F30>L(+ - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c108>t|c120>t|t135>c|a162>g|t174>a|g188>c,G63>A(- + -)|t189>a,G63>A(- + -)|g191>c,S64>T(+ - +)|a229>g,T77>A(- - -)|g261>a|g267>a|g278>a,S93>N(- - -)|g282>a|a283>g,R95>G(- - -)|a285>g,R95>G(- - -)|a303>t| t78>c| g80>a,G27>E(- - -)|c86>t,T29>I(- - -)|t88>c,F30>L(+ - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c108>t| c120>t|t135>c|a162>g| t174>a|g188>c,G63>A(- + -)|t189>a,G63>A(- + -)|g191>c,S64>T(+ - +)| a229>g,T77>A(- - -)|g261>a|g267>a|g278>a,S93>N(- - -)|g282>a|a283>g,R95>G(- - -)|a285>g,R95>G(- - -)|a303>t| 320 225 203 22 10 12 5 6 3 3 0 0 1 2 2 0 0 0 78 5 4 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 18 6 1 5 1 2 2 1 0 0 0 0 0 0 0 0 51 50 47 3 3 0 1 0 1 1 0 0 0 0 0 0 0 0 30 24 20 4 1 3 0 0 0 0 0 0 0 2 2 0 0 0 117 114 106 8 4 4 3 4 0 0 0 0 1 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctcc gaattcatccttgacagttatgcc atgagttgggtccgccaggccccagggaagggctggagtgggtctcggct attagtggaagtggtgcaaccaca tactacgcagactccgtgaagggccggttcgccatctccagagacaattccaagaacacgctatatctacaaatgaacaacctaggggccgaggacacggccgtttattactgt gcgaaagatcagtcctgtatagtgggaccttgtggttttgactac AKDQSCIVGPCGFDY IGHV3-23 IGHD1-26 IGHJ4 gaattcatccttgacagttatgcc atgagttgggtccgccaggccccagggaagggctggagtgggtctcggct attagtggaagtggtgcaaccaca tactacgcagactccgtgaagggccggttcgccatctccagagacaattccaagaacacgctatatctacaaatgaacaacctaggggccgaggacacggccgtttattactgt gcgaaagatcagtcctgtatagtgggaccttgtggttttgactac IGG1 21 212 16 5 9 11 7 10 7 2 4 8 JY8QFUQ01AC0DA 11406 productive Homsap IGHV3-48*01 F 980 95.35 205/215 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 3 8 8 13 8.8.13 [0.17.38.11] CARERGNSYGEIDYW in-frame - ctggtttcaccttcaggagctctggcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagcagtagtactactaccaaatactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagaacgtggaaacagctatggagagattgactactggggccagggaaccctggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaagactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG3 100 88 [286] a81>t|t105>g,S35>R(- - -)|a110>c,Y37>S(+ - -)|a112>g,S38>G(+ + -)|t171>c|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t194>a,I65>K(- + -)|a200>g,Y67>C(- - -)|a254>g,N85>S(- - -)| a81>t|t105>g,S35>R(- - -)|a110>c,Y37>S(+ - -)|a112>g,S38>G(+ + -)| t171>c|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t194>a,I65>K(- + -)| a200>g,Y67>C(- - -)|a254>g,N85>S(- - -)| 320 223 213 10 2 8 3 0 0 1 1 0 1 1 2 0 0 1 78 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 0 0 1 0 1 0 0 0 0 1 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 0 0 0 1 0 0 0 1 2 0 0 0 117 114 112 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ct ggtttcaccttcaggagctctggc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagcagtagtactactaccaaa tactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagaacgtggaaacagctatggagagattgactac ARERGNSYGEIDY IGHV3-48 IGHD5-18 IGHJ4 ggtttcaccttcaggagctctggc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagcagtagtactactaccaaa tactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagaacgtggaaacagctatggagagattgactac IGG3 10 213 4 6 0 2 4 8 0 2 2 6 JY8QFUQ01AC0DP 19155 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 920 90.95 201/221 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-8*02 F 1 10 7 12 10.7.12 [1.17.38.11] CARLTDWWGHFDHW in-frame - tctctggtggctccatcagcggtacttctcattactggggctgggtccgccagcccccagggaaggggctggagtggattggcagtatctactctggtgggaccacctactacaacccgtccctcaagagtcgactcaccatgtccgtcgacacgtccaagaaccagatgtccctgcggctgagctctgtgaccgccgcagacacggctgtctattactgtgcgagactgacagactggtgggggcactttgatcactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccca NA IGA1 100 100 [288] a100>g,S34>G(+ + -)|g104>c,S35>T(+ - +)|a106>t|g107>c|t109>c,Y37>H(+ - -)|c111>t,Y37>H(+ - -)|a124>g,I42>V(+ - +)|g162>c|t171>c|a173>c,Y58>S(+ - -)|a175>g,S59>G(+ + -)|g191>c,S64>T(+ - +)|g226>c,V76>L(+ - +)|a234>g,I78>M(+ + -)|a240>c|t259>a,F87>M(+ - -)|c261>g,F87>M(+ - -)|a268>c,K90>R(+ + +)|a269>g,K90>R(+ + +)|g303>c| a100>g,S34>G(+ + -)|g104>c,S35>T(+ - +)|a106>t|g107>c|t109>c,Y37>H(+ - -)|c111>t,Y37>H(+ - -)| a124>g,I42>V(+ - +)|g162>c| t171>c|a173>c,Y58>S(+ - -)|a175>g,S59>G(+ + -)|g191>c,S64>T(+ - +)| g226>c,V76>L(+ - +)|a234>g,I78>M(+ + -)|a240>c|t259>a,F87>M(+ - -)|c261>g,F87>M(+ - -)|a268>c,K90>R(+ + +)|a269>g,K90>R(+ + +)|g303>c| a320>t| 319 (320) 228 (229) 208 20 (21) 6 (7) 14 5 0 1 2 3 0 1 (2) 1 6 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 2 4 1 0 1 1 0 0 1 0 2 0 0 0 51 51 49 2 1 1 1 0 0 0 0 0 0 0 1 0 0 0 30 21 17 4 1 3 1 0 0 1 1 0 0 0 1 0 0 0 117 114 106 8 2 6 2 0 0 0 2 0 0 1 2 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcggtacttctcattac tggggctgggtccgccagcccccagggaaggggctggagtggattggcagt atctactctggtgggaccacc tactacaacccgtccctcaagagtcgactcaccatgtccgtcgacacgtccaagaaccagatgtccctgcggctgagctctgtgaccgccgcagacacggctgtctattactgt gcgagactgacagactggtgggggcactttgatcac ARLTDWWGHFDH IGHV4-39 IGHD2-8 IGHJ4 ggtggctccatcagcggtacttctcattac tggggctgggtccgccagcccccagggaaggggctggagtggattggcagt atctactctggtgggaccacc tactacaacccgtccctcaagagtcgactcaccatgtccgtcgacacgtccaagaaccagatgtccctgcggctgagctctgtgaccgccgcagacacggctgtctattactgt gcgagactgacagactggtgggggcactttgatcac IGA1 20 216 8 12 1 8 7 12 3 3 7 7 JY8QFUQ01AC0IS 10470 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 927 91.71 199/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD3-10*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKKYHYGSGTYLYYFDYW in-frame + ctctggattcacctttagccactttgccgtgacctgggtccgccaggctccagggaagggtctggaatgggtctcaactattagcggtagtgatggtagcaagtactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacaccctatatctgcaaatgaccagcctgagagccgaggacacggccgtatatttctgcgcgaagaagtatcactatggttcggggacttacttgtactattttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA2 71 100 [299] a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a110>t,Y37>F(- + -)|a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|g147>t|g153>a|g163>a,A55>T(- - -)|t171>c|g185>a,G62>D(- - -)|c194>a,T65>K(- - -)|a195>g,T65>K(- - -)|a215>g,K72>R(+ + +)|g258>c|g261>a|a275>c,N92>T(- + -)|a308>t,Y103>F(- + -)|t312>c| a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a110>t,Y37>F(- + -)| a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|g147>t|g153>a|g163>a,A55>T(- - -)| t171>c|g185>a,G62>D(- - -)|c194>a,T65>K(- - -)|a195>g,T65>K(- - -)| a215>g,K72>R(+ + +)|g258>c|g261>a|a275>c,N92>T(- + -)|a308>t,Y103>F(- + -)|t312>c| a318>g|g319>a| 317 (320) 222 (225) 204 (205) 18 (20) 6 (8) 12 3 (4) 5 (6) 0 2 2 1 2 0 2 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 1 0 1 0 0 0 0 0 51 51 46 5 2 3 1 2 0 0 0 0 0 0 1 0 1 0 30 24 20 4 1 3 1 1 0 1 0 1 0 0 0 0 0 0 117 114 108 6 3 3 1 1 0 1 1 0 1 0 1 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagccactttgcc gtgacctgggtccgccaggctccagggaagggtctggaatgggtctcaact attagcggtagtgatggtagcaag tactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacaccctatatctgcaaatgaccagcctgagagccgaggacacggccgtatatttctgc gcgaagaagtatcactatggttcggggacttacttgtactattttgactac AKKYHYGSGTYLYYFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcacctttagccactttgcc gtgacctgggtccgccaggctccagggaagggtctggaatgggtctcaact attagcggtagtgatggtagcaag tactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacaccctatatctgcaaatgaccagcctgagagccgaggacacggccgtatatttctgc gcgaagaagtatcactatggttcggggacttacttgtactattttgactac IGA2 18 213 10 8 5 9 5 9 5 1 6 6 JY8QFUQ01AC33X 18833 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*02 F or Homsap IGHV4-39*05 F (see comment) 776 83.71 185/221 nt 83.26 184/221 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-3*01 F 3 10 7 16 10.7.16 [1.17.38.11] CARHRSFGVIIVNYFETW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (83,71% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR2-IMGT at codon 39 of V-REGION: 'G' (1 nucleotides at position 37 to 37 in the user submitted sequence), (cause frameshift); tttccggtagttccatcagtagcagtgattactactGggggctgggtccgccagtccccagggaagggtctggagtggattggaagtgtctattacagggggacgcagtacctcaacccgtccctccagagtcgagtttccatttccattggcacgtccaagacgcaattctccctgagactgaggtctgtgaccgccgcagacacggctatgtattactgtgcgagacatcggtcttttggagtgataattgtgaattactttgagacctggggacagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [301] c75>t|t78>c|g82>a,G28>S(+ + -)|c84>t,G28>S(+ + -)|c93>t|t102>c|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a124>g,I42>V(+ - +)|c133>t,P45>S(+ - -)|g147>t|g162>a|a166>g,I56>V(+ - +)|t174>c|t177>g,S59>R(- - -)|g191>c,S64>T(+ - +)|c192>g,S64>T(+ - +)|a193>c,T65>Q(- - -)|c194>a,T65>Q(- - -)|c195>g,T65>Q(- - -)|t199>c,Y67>L(- - -)|a200>t,Y67>L(- - -)|a214>c,K72>Q(+ - -)|c228>t|a229>t,T77>S(+ - +)|a234>t|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|a242>g,D81>G(- - -)|a254>c,N85>T(- + -)|c255>g,N85>T(- + -)|g258>a|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c276>g,S92>R(- - -)|g301>a,V101>M(+ - -)| c75>t|t78>c| g82>a,G28>S(+ + -)|c84>t,G28>S(+ + -)|c93>t|t102>c|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| a124>g,I42>V(+ - +)|c133>t,P45>S(+ - -)|g147>t|g162>a| a166>g,I56>V(+ - +)|t174>c|t177>g,S59>R(- - -)|g191>c,S64>T(+ - +)|c192>g,S64>T(+ - +)|a193>c,T65>Q(- - -)|c194>a,T65>Q(- - -)|c195>g,T65>Q(- - -)| t199>c,Y67>L(- - -)|a200>t,Y67>L(- - -)|a214>c,K72>Q(+ - -)|c228>t|a229>t,T77>S(+ - +)|a234>t|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|a242>g,D81>G(- - -)|a254>c,N85>T(- + -)|c255>g,N85>T(- + -)|g258>a|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c276>g,S92>R(- - -)|g301>a,V101>M(+ - -)| 320 229 193 36 10 26 5 7 5 4 3 1 4 0 1 4 1 1 78 5 3 2 2 0 0 0 1 1 0 0 0 0 0 0 0 0 36 30 24 6 2 4 1 2 2 1 0 0 0 0 0 0 0 0 51 51 47 4 2 2 1 1 1 0 0 0 0 0 0 0 1 0 30 21 13 8 1 7 1 0 0 1 1 1 0 0 1 2 0 1 117 114 98 16 3 13 2 4 1 1 2 0 4 0 0 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tttcc ggtagttccatcagtagcagtgattactac tggggctgggtccgccagtccccagggaagggtctggagtggattggaagt gtctattacagggggacgcag tacctcaacccgtccctccagagtcgagtttccatttccattggcacgtccaagacgcaattctccctgagactgaggtctgtgaccgccgcagacacggctatgtattactgt gcgagacatcggtcttttggagtgataattgtgaattactttgagacc ARHRSFGVIIVNYFET IGHV4-39 IGHD3-3 IGHJ4 ggtagttccatcagtagcagtgattactac tggggctgggtccgccagtccccagggaagggtctggagtggattggaagt gtctattacagggggacgcag tacctcaacccgtccctccagagtcgagtttccatttccattggcacgtccaagacgcaattctccctgagactgaggtctgtgaccgccgcagacacggctatgtattactgt gcgagacatcggtcttttggagtgataattgtgaattactttgagacc IGA1 34 216 19 15 11 18 8 16 5 3 15 11 JY8QFUQ01AC5GC 13695 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 902 90.05 199/221 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 2 10 7 12 10.7.12 [1.17.38.11] CARHSRLVRYFEFW in-frame + tctctggtggctccgtcagcagtggtaattactactggaactggatccgccagtccccagggaaggggctggagtggattgggagcgtctcctatggtgggaacacctactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgtgcgagacattcgagactggtacggtactttgaattctggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [288] a88>g,I30>V(+ - +)|a103>g,S35>G(+ + -)|g107>a,S36>N(- - -)|g118>a,G40>N(- - -)|g119>a,G40>N(- - -)|c133>t,P45>S(+ - -)|t165>c|a166>g,I56>V(+ - +)|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a175>g,S59>G(+ + -)|g191>a,S64>N(- - -)|a215>t,K72>M(- + -)|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|c236>t,S79>F(- - -)|a240>c|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c261>g,F87>L(+ - -)|c271>g,L91>V(+ - +)|a291>g| a88>g,I30>V(+ - +)|a103>g,S35>G(+ + -)|g107>a,S36>N(- - -)| g118>a,G40>N(- - -)|g119>a,G40>N(- - -)|c133>t,P45>S(+ - -)|t165>c| a166>g,I56>V(+ - +)|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a175>g,S59>G(+ + -)|g191>a,S64>N(- - -)| a215>t,K72>M(- + -)|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|c236>t,S79>F(- - -)|a240>c|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c261>g,F87>L(+ - -)|c271>g,L91>V(+ - +)|a291>g| 320 229 207 22 3 19 7 4 3 2 2 1 1 0 0 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 2 1 0 0 0 0 0 0 0 0 0 0 51 51 47 4 1 3 0 2 1 1 0 0 0 0 0 0 0 0 30 21 16 5 0 5 2 1 0 1 1 0 0 0 0 0 0 0 117 114 104 10 2 8 3 0 2 0 1 1 1 0 0 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcagcagtggtaattactac tggaactggatccgccagtccccagggaaggggctggagtggattgggagc gtctcctatggtgggaacacc tactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgt gcgagacattcgagactggtacggtactttgaattc ARHSRLVRYFEF IGHV4-39 IGHD5-18 IGHJ4 ggtggctccgtcagcagtggtaattactac tggaactggatccgccagtccccagggaaggggctggagtggattgggagc gtctcctatggtgggaacacc tactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgt gcgagacattcgagactggtacggtactttgaattc IGA1 22 216 16 6 7 10 9 12 3 0 11 8 JY8QFUQ01AC792 18264 productive Homsap IGHV3-48*02 F 981 94.93 206/217 nt NA Homsap IGHJ6*02 F 238 87.1 54/62 nt Homsap IGHD2-21*02 F 2 8 8 19 8.8.19 [1.17.38.11] CARPAGEGDPFFHYYSGLDVW in-frame + ctctggattcaccttcagtagttatagcatgaactgggtccgccaggctccagggaaggggctggagtggatttcatacattagtggtagtggcggtaccatatactatgcagactctgtgaagggccgcttcaccatctccagagacaatgccaagaagtcactgtatcttcaaatggacagcctgagagacgaggacacggctgtgtattactgtgcgagacccgcgggggagggtgacccttttttccactactactccggtttggacgtctggggccaggggaccacggtcatcgtctcgtcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [306] c108>t|g157>a,V53>I(+ - +)|a172>g,S58>G(+ + -)|a184>g,S62>G(+ + -)|t186>c,S62>G(+ + -)|a187>g,S63>G(+ + -)|c201>t|a225>c|c255>g,N85>K(+ - -)|g267>t|a274>g,N92>D(+ + -)| c108>t| g157>a,V53>I(+ - +)| a172>g,S58>G(+ + -)|a184>g,S62>G(+ + -)|t186>c,S62>G(+ + -)|a187>g,S63>G(+ + -)| c201>t|a225>c|c255>g,N85>K(+ - -)|g267>t|a274>g,N92>D(+ + -)| g319>c|a320>c| 318 (320) 223 (225) 212 11 (13) 4 (6) 7 4 1 2 1 1 (2) 0 0 0 0 (1) 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 3 0 0 1 0 0 0 0 0 0 0 0 117 114 109 5 3 2 1 0 1 0 1 0 0 0 0 1 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagttatagc atgaactgggtccgccaggctccagggaaggggctggagtggatttcatac attagtggtagtggcggtaccata tactatgcagactctgtgaagggccgcttcaccatctccagagacaatgccaagaagtcactgtatcttcaaatggacagcctgagagacgaggacacggctgtgtattactgt gcgagacccgcgggggagggtgacccttttttccactactactccggtttggacgtc ARPAGEGDPFFHYYSGLDV IGHV3-48 IGHD2-21 IGHJ6 ggattcaccttcagtagttatagc atgaactgggtccgccaggctccagggaaggggctggagtggatttcatac attagtggtagtggcggtaccata tactatgcagactctgtgaagggccgcttcaccatctccagagacaatgccaagaagtcactgtatcttcaaatggacagcctgagagacgaggacacggctgtgtattactgt gcgagacccgcgggggagggtgacccttttttccactactactccggtttggacgtc IGG1 11 213 8 3 3 5 5 6 3 1 3 4 JY8QFUQ01AC7WH 12530 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 999 95.85 208/217 nt NA Homsap IGHJ5*02 F 237 96.08 49/51 nt Homsap IGHD6-6*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVRSEYSSSNWFDPW in-frame - ctctggattcaccttcagtagctatagaatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattgatagtagtagtcacaacatatactacagagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgtgagatccgagtatagcagttcaaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [287] c114>a,S38>R(- - -)|a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a187>c,S63>H(+ - -)|g188>a,S63>H(+ - -)|t189>c,S63>H(+ - -)|t190>a,Y64>N(- - -)|g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|c314>t,A105>V(+ - +)| c114>a,S38>R(- - -)| a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a187>c,S63>H(+ - -)|g188>a,S63>H(+ - -)|t189>c,S63>H(+ - -)|t190>a,Y64>N(- - -)| g202>a,A68>R(- - -)|c203>g,A68>R(- - -)| c314>t,A105>V(+ - +)|g319>t|a320>c| 318 (320) 223 (225) 213 10 (12) 0 (2) 10 1 3 1 1 1 (2) 1 0 1 0 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 18 6 0 6 1 2 0 1 1 0 0 1 0 0 0 0 117 114 112 2 0 2 0 1 0 0 0 0 0 0 0 1 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctataga atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attgatagtagtagtcacaacata tactacagagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gtgagatccgagtatagcagttcaaactggttcgacccc VRSEYSSSNWFDP IGHV3-21 IGHD6-6 IGHJ5 ggattcaccttcagtagctataga atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attgatagtagtagtcacaacata tactacagagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gtgagatccgagtatagcagttcaaactggttcgacccc IGA2 9 213 5 4 3 5 2 4 0 0 2 7 JY8QFUQ01ACBLB 11639 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 797 85.32 186/218 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD5-18*01 F 2 8 8 16 8.8.16 [1.17.38.11] CAKVWIELWSAGDHFDNW in-frame + cctctggattcatatttactagatatgccatgacctgggtccgccaggctccagggaagggtctggagtgggtcgcttctatcagtggtagtgggattagtaaaaagtacgcagacggcgtggagggccgattcaccatctccagagacagttccgagagaacactgtatctacaaatgaacagcctgagagtcgaggacacggccacatattattgtgcgaaagtatggatagagttatggtcggctggggaccactttgacaactggggccagggaaccctggtcaacgtctcctcagcatcccctgaccagccccaaggtcttcccgctgagcctcgacagcacccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 71 84 [298, 300] c86>t,T29>I(- - -)|c87>a,T29>I(- - -)|g104>c,S35>T(+ - +)|c105>t,S35>T(+ - +)|c108>a,S36>R(- - -)|g119>c,S40>T(+ - +)|g147>t|t160>g,S54>A(- + -)|a162>t,S54>A(- + -)|g163>t,A55>S(- + -)|t168>c|t186>g|g187>a,G63>I(- - -)|g188>t,G63>I(- - -)|c192>t|c194>a,T65>K(- - -)|t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|t208>g,S70>G(+ + -)|c209>g,S70>G(+ + -)|a214>g,K72>E(+ - -)|g225>a|a245>g,N82>S(- - -)|a250>g,K84>E(+ - -)|a254>g,N85>R(+ - -)|c255>a,N85>R(+ - -)|g258>a|g267>a|c287>t,A96>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|c309>t| c86>t,T29>I(- - -)|c87>a,T29>I(- - -)|g104>c,S35>T(+ - +)|c105>t,S35>T(+ - +)|c108>a,S36>R(- - -)| g119>c,S40>T(+ - +)|g147>t|t160>g,S54>A(- + -)|a162>t,S54>A(- + -)|g163>t,A55>S(- + -)| t168>c|t186>g|g187>a,G63>I(- - -)|g188>t,G63>I(- - -)|c192>t|c194>a,T65>K(- - -)| t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|t208>g,S70>G(+ + -)|c209>g,S70>G(+ + -)|a214>g,K72>E(+ - -)|g225>a|a245>g,N82>S(- - -)|a250>g,K84>E(+ - -)|a254>g,N85>R(+ - -)|c255>a,N85>R(+ - -)|g258>a|g267>a|c287>t,A96>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|c309>t| a320>t| 319 (320) 225 (226) 193 32 (33) 8 (9) 24 4 5 5 2 0 4 1 (2) 1 2 2 3 3 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 0 2 0 0 2 0 0 1 0 0 0 51 51 46 5 1 4 0 0 0 0 0 0 1 0 1 0 2 1 30 24 18 6 3 3 0 1 1 1 0 1 0 0 0 0 1 1 117 114 98 16 4 12 4 4 2 1 0 1 0 1 0 2 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcatatttactagatatgcc atgacctgggtccgccaggctccagggaagggtctggagtgggtcgcttct atcagtggtagtgggattagtaaa aagtacgcagacggcgtggagggccgattcaccatctccagagacagttccgagagaacactgtatctacaaatgaacagcctgagagtcgaggacacggccacatattattgt gcgaaagtatggatagagttatggtcggctggggaccactttgacaac AKVWIELWSAGDHFDN IGHV3-23 IGHD5-18 IGHJ4 ggattcatatttactagatatgcc atgacctgggtccgccaggctccagggaagggtctggagtgggtcgcttct atcagtggtagtgggattagtaaa aagtacgcagacggcgtggagggccgattcaccatctccagagacagttccgagagaacactgtatctacaaatgaacagcctgagagtcgaggacacggccacatattattgt gcgaaagtatggatagagttatggtcggctggggaccactttgacaac IGA2 32 213 16 16 10 21 6 11 5 3 16 8 JY8QFUQ01ACCKZ 15026 productive (see comment) Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 904 91.2 197/216 nt 90.28 195/216 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD1-7*01 F 3 8 7 15 8.7.15 [2.16.38.11] CIADLPDSAENYPFDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 61 in the user submitted sequence), (cause frameshift)in CDR2-IMGT, from codon 58 of V-REGION: 9 nucleotides (from position 90 in the user submitted sequence), (do not cause frameshift) agcctctggattcacttttagtggcgcctggatgagctgggtccgccaggctccagggaagggctggagtgggttggccgtgttagaagcggtgggacaacagactaccctgcacccgtggaaggcagattcaccatctctagagatgatcgaaaaaacacgttgtatctggaaataagtagcctgaaaatcgaagacacagccgtatattactgtatcgcagatttgcccgactcggcagagaactatccctttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [292] c90>t|a106>g,N36>G(- - -)|a107>g,N36>G(- - -)|a166>g,I56>V(+ - +)|a170>g,K57>R(+ + +)|t183>c|g202>c,A68>P(- - -)|a214>g,K72>E(+ - -)|a237>t|t247>c,S83>R(- - -)|c248>g,S83>R(- - -)|c259>t|c268>g,Q90>E(+ + -)|g273>a,M91>I(+ + -)|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|c287>t,T96>I(- - -)|g291>a|g303>a|c314>t,T105>I(- - -)|a316>g,T106>A(- - -)| c90>t|a106>g,N36>G(- - -)|a107>g,N36>G(- - -)| a166>g,I56>V(+ - +)|a170>g,K57>R(+ + +)|t183>c| g202>c,A68>P(- - -)|a214>g,K72>E(+ - -)|a237>t|t247>c,S83>R(- - -)|c248>g,S83>R(- - -)|c259>t|c268>g,Q90>E(+ + -)|g273>a,M91>I(+ + -)|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|c287>t,T96>I(- - -)|g291>a|g303>a| c314>t,T105>I(- - -)|a316>g,T106>A(- - -)| 320 224 203 21 6 15 7 3 5 2 0 0 1 0 1 2 0 0 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 2 0 1 0 0 0 0 0 0 0 0 0 51 50 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 18 3 1 2 2 0 0 1 0 0 0 0 0 0 0 0 117 114 101 13 4 9 2 3 3 1 0 0 1 0 1 2 0 0 8 8 6 2 0 2 1 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| agcctct ggattcacttttagtggcgcctgg atgagctgggtccgccaggctccagggaagggctggagtgggttggccgt gttagaagcggtgggacaaca gactaccctgcacccgtggaaggcagattcaccatctctagagatgatcgaaaaaacacgttgtatctggaaataagtagcctgaaaatcgaagacacagccgtatattactgt atcgcagatttgcccgactcggcagagaactatccctttgactac IADLPDSAENYPFDY IGHV3-15 IGHD1-7 IGHJ4 ggattcacttttagtggcgcctgg atgagctgggtccgccaggctccagggaagggctggagtgggttggccgt gttagaagcggtgggacaaca gactaccctgcacccgtggaaggcagattcaccatctctagagatgatcgaaaaaacacgttgtatctggaaataagtagcctgaaaatcgaagacacagccgtatattactgt atcgcagatttgcccgactcggcagagaactatccctttgactac IGA2 19 209 15 4 7 10 8 9 4 2 9 4 JY8QFUQ01ACENJ 10708 productive Homsap IGHV3-21*04 F 985 95.37 206/216 nt NA Homsap IGHJ6*02 F, or Homsap IGHJ6*03 F 172 78.57 44/56 nt Homsap IGHD6-13*01 F 3 8 8 10 8.8.10 [1.17.38.11] CARAVSNQVDVW in-frame + tctggattcgccttcagtacatatatcatgaattgggtccgccaggctccagggaaggggctggagtgggtctcatccattagttttagtagtgattacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgacgacacggccgtgtattactgtgcgagagccgtttcgaatcaggtggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [277] a85>g,T29>A(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|g113>t,S38>I(- - -)|c120>t|a172>t,S58>F(- - -)|g173>t,S58>F(- - -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|g291>c,E97>D(+ - +)| a85>g,T29>A(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|g113>t,S38>I(- - -)| c120>t| a172>t,S58>F(- - -)|g173>t,S58>F(- - -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)| g291>c,E97>D(+ - +)| a320>c| 319 (320) 223 (224) 213 10 (11) 1 (2) 9 2 1 1 0 0 (1) 1 1 0 2 0 2 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 0 0 0 0 1 0 0 1 0 1 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 1 0 0 0 0 1 0 0 0 1 0 117 114 113 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcgccttcagtacatatatc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagttttagtagtgattacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgacgacacggccgtgtattactgt gcgagagccgtttcgaatcaggtggacgtc ARAVSNQVDV IGHV3-21 IGHD6-13 IGHJ6 ggattcgccttcagtacatatatc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagttttagtagtgattacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgacgacacggccgtgtattactgt gcgagagccgtttcgaatcaggtggacgtc IGA1 10 213 4 6 2 7 2 3 1 0 1 8 JY8QFUQ01ACFOO 12827 productive Homsap IGHV3-9*01 F 1017 96.77 210/217 nt NA Homsap IGHJ4*02 F 240 100 48/48 nt Homsap IGHD3-22*01 F 2 8 8 15 8.8.15 [1.17.38.11] CAKGDSGGYTHNYFDYW in-frame + ctctggattcaggtttgatgattatgccatgcactgggtccggcaagctccagggaagggcctggagtgggtctcaggtattagctggaatagtggtagtatagggtatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgcgacctgaggacacggccttgtattactgtgcaaaaggggatagtggtggttatacccacaactactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [293] c86>g,T29>R(- - -)|c87>g,T29>R(- - -)|t171>c|c192>t|c198>g|a283>c|g286>c,A96>P(- - -)| c86>g,T29>R(- - -)|c87>g,T29>R(- - -)| t171>c|c192>t| c198>g|a283>c|g286>c,A96>P(- - -)| a320>g,D107>G(- - -)|t321>g,D107>G(- - -)|a322>g| 319 (322) 224 (227) 217 7 (10) 4 (5) 3 (5) 0 (2) 0 1 1 1 0 0 0 1 3 0 0 (1) 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 0 0 0 2 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 2 0 0 0 1 1 0 0 0 0 0 0 0 0 117 114 111 3 2 1 0 0 0 0 1 0 0 0 1 1 0 0 7 (10) 7 7 0 (3) 0 (1) 0 (2) 0 (2) 0 0 0 0 0 0 0 0 0 0 0 (1) aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaggtttgatgattatgcc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaggt attagctggaatagtggtagtata gggtatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgcgacctgaggacacggccttgtattactgt gcaaaaggggatagtggtggttatacccacaactactttgactac AKGDSGGYTHNYFDY IGHV3-9 IGHD3-22 IGHJ4 ggattcaggtttgatgattatgcc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaggt attagctggaatagtggtagtata gggtatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgcgacctgaggacacggccttgtattactgt gcaaaaggggatagtggtggttatacccacaactactttgactac IGA1 7 213 2 5 1 5 1 2 2 2 1 2 JY8QFUQ01ACH9Z 8559 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 887 89.91 196/218 nt NA Homsap IGHJ6*02 F (see comment) 175 75.81 47/62 nt Homsap IGHD3-10*01 F 2 8 8 21 8.8.21 [1.17.38.11] CARVMREHYYGAGNYPWGGMEVW in-frame - Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) or Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) cctctggattcacctttaacaactatgccatgacctgggtccgccaggctccggggaaggggctggagtgggtctcagctatcagtggcagtggcggtaccacttactacgcagactccgtgcagggccgcttcaccatttccagagacaatcacgaaaacaccctgtatctggaaatgagtagcctgagagccgaggacgcggccgtctattactgtgcgagagtgatgagggaacattattatggagcggggaattatccctgggggggaatggaagtctggggccaggggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [312] g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|a138>g|t168>c|t174>c|t186>c|g191>c,S64>T(+ - +)|a195>t|a214>c,K72>Q(+ - -)|g225>c|c234>t|t247>c,S83>H(+ - -)|c248>a,S83>H(+ - -)|a250>g,K84>E(+ - -)|g252>a,K84>E(+ - -)|g258>c|c268>g,Q90>E(+ + -)|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|a295>g,T99>A(- - -)|a303>c|a317>g,K106>R(+ + +)| g104>a,S35>N(- - -)|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|a138>g| t168>c|t174>c|t186>c|g191>c,S64>T(+ - +)|a195>t| a214>c,K72>Q(+ - -)|g225>c|c234>t|t247>c,S83>H(+ - -)|c248>a,S83>H(+ - -)|a250>g,K84>E(+ - -)|g252>a,K84>E(+ - -)|g258>c|c268>g,Q90>E(+ + -)|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|a295>g,T99>A(- - -)|a303>c| a317>g,K106>R(+ + +)|a320>t| 319 (320) 225 (226) 202 23 (24) 9 (10) 14 5 3 2 4 2 1 1 (2) 0 4 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 2 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 1 0 0 0 0 0 0 0 1 0 0 0 30 24 19 5 4 1 0 0 0 3 0 0 1 0 1 0 0 0 117 114 101 13 4 9 3 1 2 1 2 1 0 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 1 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttaacaactatgcc atgacctgggtccgccaggctccggggaaggggctggagtgggtctcagct atcagtggcagtggcggtaccact tactacgcagactccgtgcagggccgcttcaccatttccagagacaatcacgaaaacaccctgtatctggaaatgagtagcctgagagccgaggacgcggccgtctattactgt gcgagagtgatgagggaacattattatggagcggggaattatccctgggggggaatggaagtc ARVMREHYYGAGNYPWGGMEV IGHV3-23 IGHD3-10 IGHJ6 ggattcacctttaacaactatgcc atgacctgggtccgccaggctccggggaaggggctggagtgggtctcagct atcagtggcagtggcggtaccact tactacgcagactccgtgcagggccgcttcaccatttccagagacaatcacgaaaacaccctgtatctggaaatgagtagcctgagagccgaggacgcggccgtctattactgt gcgagagtgatgagggaacattattatggagcggggaattatccctgggggggaatggaagtc IGA1 22 213 13 9 5 11 8 11 5 4 10 3 JY8QFUQ01ACHS4 15076 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 935 93.02 200/215 nt 92.56 199/215 nt Homsap IGHJ2*01 F 211 88.68 47/53 nt Homsap IGHD4-23*01 ORF 2 8 7 17 8.7.17 [1.17.38.11] CARFHDYGGYGFYYYSDFW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 49 to 49 in the user submitted sequence), (cause frameshift); tctctggtggctccatcagtagtcattactggagctggatccggcagcCccccaggggagggactggagtggattggctatatctctgacagtggaagcaccaattacaacccctccctcaagagtcgagtcactatatcagtagacacgtccgagaggcagatctccctgaagctgacctctgtgaccgctgcggacacggccgtatattactgtgcgagattccatgactacggaggttacgggttttactattactccgatttctggggccgtggcaccctggtcacggtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [298] t109>c,Y37>H(+ - -)|c111>t,Y37>H(+ - -)|a142>g,K48>E(+ - -)|g162>c|a170>c,Y57>S(+ - -)|t172>g,Y58>D(- - -)|g189>a|c198>t|c231>t|a250>g,K84>E(+ - -)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)|t259>a,F87>I(+ - -)|g275>c,S92>T(+ - +)|g303>a| t109>c,Y37>H(+ - -)|c111>t,Y37>H(+ - -)| a142>g,K48>E(+ - -)|g162>c| a170>c,Y57>S(+ - -)|t172>g,Y58>D(- - -)|g189>a| c198>t|c231>t|a250>g,K84>E(+ - -)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)|t259>a,F87>I(+ - -)|g275>c,S92>T(+ - +)|g303>a| g319>t|a320>t| 318 (320) 221 (223) 206 15 (17) 5 (7) 10 3 2 3 1 1 0 0 (1) 1 2 1 0 (1) 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 1 1 0 0 0 0 0 0 0 0 51 51 49 2 1 1 1 0 0 0 0 0 0 0 1 0 0 0 30 21 18 3 1 2 0 1 0 0 1 0 0 0 0 0 0 1 117 114 106 8 3 5 2 1 2 0 0 0 0 1 1 1 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagtagtcattac tggagctggatccggcagcccccaggggagggactggagtggattggctat atctctgacagtggaagcacc aattacaacccctccctcaagagtcgagtcactatatcagtagacacgtccgagaggcagatctccctgaagctgacctctgtgaccgctgcggacacggccgtatattactgt gcgagattccatgactacggaggttacgggttttactattactccgatttc ARFHDYGGYGFYYYSDF IGHV4-59 IGHD4-23 IGHJ2 ggtggctccatcagtagtcattac tggagctggatccggcagcccccaggggagggactggagtggattggctat atctctgacagtggaagcacc aattacaacccctccctcaagagtcgagtcactatatcagtagacacgtccgagaggcagatctccctgaagctgacctctgtgaccgctgcggacacggccgtatattactgt gcgagattccatgactacggaggttacgggttttactattactccgatttc IGA2 15 210 9 6 5 8 4 7 4 1 6 4 JY8QFUQ01ACLAL 11356 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 809 86.51 186/215 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-15*01 F 2 8 7 10 8.7.10 [1.17.38.11] CATLRGGTFDYW in-frame - tctctggtgcctccatcaggagttattattggagttggatccggcagcccccaggaaagggactggagtggattggttatattaattatgttggggacaccgattacaacccctccctcaagagtcgagtctccatgtcagcagccacgtccaagaaccaggtcttcctgcagctgacctctgtgaccgctgcggacaccgcctattatttctgtgcgacactgagagggggtactttcgactactggggccggggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [277] g83>c,G28>A(- + -)|t105>g,S35>R(- - -)|c111>t|c114>t|c120>t|g141>a|g162>t|c168>t|t169>a,Y57>N(- - -)|c174>t|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a196>g,N66>D(+ + -)|c198>t,N66>D(+ + -)|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|t239>c,V80>A(+ - +)|a242>c,D81>A(- - -)|t259>g,F87>V(+ - -)|c263>t,S88>F(- - -)|a268>c,K90>Q(+ - -)|g275>c,S92>T(+ - +)|g297>c|g301>t,V101>Y(- - -)|t302>a,V101>Y(- - -)|g303>t,V101>Y(- - -)|a308>t,Y103>F(- + -)| g83>c,G28>A(- + -)|t105>g,S35>R(- - -)|c111>t|c114>t| c120>t|g141>a|g162>t| c168>t|t169>a,Y57>N(- - -)|c174>t|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a196>g,N66>D(+ + -)|c198>t,N66>D(+ + -)|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|t239>c,V80>A(+ - +)|a242>c,D81>A(- - -)|t259>g,F87>V(+ - -)|c263>t,S88>F(- - -)|a268>c,K90>Q(+ - -)|g275>c,S92>T(+ - +)|g297>c|g301>t,V101>Y(- - -)|t302>a,V101>Y(- - -)|g303>t,V101>Y(- - -)|a308>t,Y103>F(- + -)| g317>c,R106>T(- - -)|g319>c|a320>t| 316 (320) 219 (223) 190 (191) 29 (32) 8 (10) 21 (22) 4 2 7 1 2 0 2 (3) 2 3 (5) 0 4 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 0 2 0 0 0 0 0 1 0 0 1 51 51 48 3 3 0 0 1 1 0 0 0 0 0 0 0 1 0 30 21 14 7 2 5 2 1 2 0 0 0 0 1 0 0 1 0 117 114 99 15 1 14 2 0 2 1 2 0 2 1 2 0 2 1 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 0 0 0 0 0 0 (1) 0 0 (2) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgcctccatcaggagttattat tggagttggatccggcagcccccaggaaagggactggagtggattggttat attaattatgttggggacacc gattacaacccctccctcaagagtcgagtctccatgtcagcagccacgtccaagaaccaggtcttcctgcagctgacctctgtgaccgctgcggacaccgcctattatttctgt gcgacactgagagggggtactttcgactac ATLRGGTFDY IGHV4-59 IGHD2-15 IGHJ4 ggtgcctccatcaggagttattat tggagttggatccggcagcccccaggaaagggactggagtggattggttat attaattatgttggggacacc gattacaacccctccctcaagagtcgagtctccatgtcagcagccacgtccaagaaccaggtcttcctgcagctgacctctgtgaccgctgcggacaccgcctattatttctgt gcgacactgagagggggtactttcgactac IGG1 29 210 14 15 9 16 5 13 4 4 14 7 JY8QFUQ01ACLRQ 5876 productive Homsap IGHV4-4*02 F, or Homsap IGHV4-4*03 F or Homsap IGHV4-4*04 F or Homsap IGHV4-4*05 F 932 92.2 201/218 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD5-12*01 F 2 9 7 11 9.7.11 [1.17.38.11] CARASTLRYLDRW in-frame - tctctggtggctccatcagcagtgataattggtggagttgggtccgccagcccccagggaagggactggaatggattggggaaatatatcatagtgggagcacctactacaacccgtccctcaagagtcgagtcaccatatccctagacaagtccaagagtcaattcttcctggagctgaggtctgtgaccgccgcggacacggccgtatattattgtgcgagagcctccacactacgatatcttgaccgctggggccagggaaccccggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [282] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c111>t|g147>a|g153>a|c168>a|a196>t,N66>Y(- - -)|a237>c|g238>c,V80>L(+ - +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|c263>t,S88>F(- - -)|a268>g,K90>E(+ - -)|c276>g,S92>R(- - -)|g303>a|c309>t| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c111>t| g147>a|g153>a| c168>a| a196>t,N66>Y(- - -)|a237>c|g238>c,V80>L(+ - +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|c263>t,S88>F(- - -)|a268>g,K90>E(+ - -)|c276>g,S92>R(- - -)|g303>a|c309>t| a320>c| 319 (320) 225 (226) 208 17 (18) 8 (9) 9 3 5 4 0 1 (2) 1 1 0 1 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 27 24 3 1 2 1 1 1 0 0 0 0 0 0 0 0 0 51 51 49 2 2 0 0 2 0 0 0 0 0 0 0 0 0 0 30 21 20 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 117 114 103 11 4 7 2 2 3 0 1 0 1 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcagtgataattgg tggagttgggtccgccagcccccagggaagggactggaatggattggggaa atatatcatagtgggagcacc tactacaacccgtccctcaagagtcgagtcaccatatccctagacaagtccaagagtcaattcttcctggagctgaggtctgtgaccgccgcggacacggccgtatattattgt gcgagagcctccacactacgatatcttgaccgc ARASTLRYLDR IGHV4-4 IGHD5-12 IGHJ4 ggtggctccatcagcagtgataattgg tggagttgggtccgccagcccccagggaagggactggaatggattggggaa atatatcatagtgggagcacc tactacaacccgtccctcaagagtcgagtcaccatatccctagacaagtccaagagtcaattcttcctggagctgaggtctgtgaccgccgcggacacggccgtatattattgt gcgagagcctccacactacgatatcttgaccgc IGA1 17 213 12 5 9 12 3 5 6 2 7 2 JY8QFUQ01ACLU1 8596 productive (see comment) Homsap IGHV3-7*01 F 1022 96.79 211/218 nt 96.33 210/218 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSGFYSTGYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 13 to 13 in the user submitted sequence), (cause frameshift); cctctggattcaGcctttagtacctattggatgggctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaaacaagatggaagtgacaaatactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtctattactgtgcgattagtagtggtttttactcgacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [283] g107>c,S36>T(+ - +)|a118>g,S40>G(+ + -)|g171>a|g192>c,E64>D(+ - +)|c248>g,A83>G(- + -)|c265>t|g303>c| g107>c,S36>T(+ - +)| a118>g,S40>G(+ + -)| g171>a|g192>c,E64>D(+ - +)| c248>g,A83>G(- + -)|c265>t|g303>c| g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 316 (320) 222 (226) 215 7 (11) 3 (5) 4 (6) 1 (2) 1 (2) 1 0 0 0 0 (1) 0 3 1 0 (1) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 50 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 117 114 111 3 2 1 0 0 1 0 0 0 0 0 1 1 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagtacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaacaagatggaagtgacaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgattagtagtggtttttactcgacgggctac AISSGFYSTGY IGHV3-7 IGHD3-22 IGHJ4 ggattcacctttagtacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaacaagatggaagtgacaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgattagtagtggtttttactcgacgggctac IGA2 7 213 3 4 2 6 1 1 2 1 2 2 JY8QFUQ01ACRP3 12281 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*04 F or Homsap IGHV3-30*14 F or Homsap IGHV3-30*18 F or Homsap IGHV3-30-3*03 F or Homsap IGHV3-30-5*01 F 950 93.12 203/218 nt NA Homsap IGHJ6*02 F (see comment) 193 79.03 49/62 nt Homsap IGHD5-24*01 ORF 3 8 8 18 8.8.18 [1.17.38.11] CARKGPRRDGSLQQCDMDVW in-frame + Other possibilities: Homsap_IGHJ4*03 (shorter alignment but highest percentage of identity) cctctggattcatcttcagcaaccttgcgatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaattatatcatatgatggaggtattaagtactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctacaaatgaacaacctgagacttgaggacacggctgtgtattactgtgcgagaaaaggccccagaagagatggctccctacagcaatgcgatatggacgtctggggccaagggaccacgatcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 89 [303, 302] c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)|t109>c,Y37>L(- - -)|a110>t,Y37>L(- - -)|g113>c,G38>A(- + -)|c114>g,G38>A(- + -)|g163>a,V55>I(+ - +)|a187>g,S63>G(+ + -)|a191>t,N64>I(- - -)|a195>g|g267>a|g278>a,S93>N(- - -)|g286>c,A96>L(+ - +)|c287>t,A96>L(+ - +)| c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)|t109>c,Y37>L(- - -)|a110>t,Y37>L(- - -)|g113>c,G38>A(- + -)|c114>g,G38>A(- + -)| g163>a,V55>I(+ - +)| a187>g,S63>G(+ + -)|a191>t,N64>I(- - -)|a195>g| g267>a|g278>a,S93>N(- - -)|g286>c,A96>L(+ - +)|c287>t,A96>L(+ - +)| g319>a| 318 (320) 224 (226) 209 (210) 15 (16) 3 (4) 12 2 4 (5) 2 2 0 0 2 0 2 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 1 6 0 1 1 2 0 0 1 0 1 1 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 2 0 0 0 0 0 1 0 0 0 0 0 117 114 110 4 1 3 0 2 1 0 0 0 0 0 1 0 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcatcttcagcaaccttgcg atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatt atatcatatgatggaggtattaag tactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctacaaatgaacaacctgagacttgaggacacggctgtgtattactgt gcgagaaaaggccccagaagagatggctccctacagcaatgcgatatggacgtc ARKGPRRDGSLQQCDMDV IGHV3-30 IGHD5-24 IGHJ6 ggattcatcttcagcaaccttgcg atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatt atatcatatgatggaggtattaag tactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctacaaatgaacaacctgagacttgaggacacggctgtgtattactgt gcgagaaaaggccccagaagagatggctccctacagcaatgcgatatggacgtc IGA1 15 213 10 5 6 9 4 6 1 2 4 8 JY8QFUQ01ACRUV 12750 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 881 90.23 194/215 nt NA Homsap IGHJ6*02 F (see comment) 202 80.65 50/62 nt Homsap IGHD2-21*02 F 3 8 7 16 8.7.16 [1.17.38.11] CAREFRAPSLARYGMDVW in-frame - Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) cctcggggttcagcgtcagtaataacttcatgacctgggtccgccaggttccagggaaggggctggagtgggtctcagttatttatagcaatggtgaaacaatctacgcagactccgtgaagggccgattcactatgtccagagacaattccaagaacacactgtttcttcaaatgaacagcctgagaggcgaggacacggccgtgtaccactgtgcgagagaatttcgggcgccttctttggccagatacggtatggatgtctggggccacgggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [294] t78>g|c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a113>t,Y38>F(- + -)|g119>c,S40>T(+ - +)|c134>t,A45>V(+ - +)|g175>a,G59>N(- - -)|g176>a,G59>N(- - -)|a190>g,S64>E(- - -)|g191>a,S64>E(- - -)|c192>a,S64>E(- - -)|t196>a,Y66>I(- - -)|a197>t,Y66>I(- - -)|c231>t|c234>g,I78>M(+ + -)|g258>a|a263>t,Y88>F(- + -)|c287>g,A96>G(- + -)|t306>c|t307>c,Y103>H(+ - -)| t78>g| c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a113>t,Y38>F(- + -)| g119>c,S40>T(+ - +)|c134>t,A45>V(+ - +)| g175>a,G59>N(- - -)|g176>a,G59>N(- - -)|a190>g,S64>E(- - -)|g191>a,S64>E(- - -)|c192>a,S64>E(- - -)| t196>a,Y66>I(- - -)|a197>t,Y66>I(- - -)|c231>t|c234>g,I78>M(+ + -)|g258>a|a263>t,Y88>F(- + -)|c287>g,A96>G(- + -)|t306>c|t307>c,Y103>H(+ - -)| 320 223 202 21 4 17 1 5 3 2 0 1 3 1 1 3 0 1 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 36 24 20 4 0 4 0 1 1 0 0 0 1 0 0 1 0 0 51 51 49 2 0 2 0 0 1 0 0 0 0 0 1 0 0 0 30 21 16 5 0 5 1 3 0 0 0 1 0 0 0 0 0 0 117 114 105 9 3 6 0 1 1 2 0 0 2 1 0 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctcg gggttcagcgtcagtaataacttc atgacctgggtccgccaggttccagggaaggggctggagtgggtctcagtt atttatagcaatggtgaaaca atctacgcagactccgtgaagggccgattcactatgtccagagacaattccaagaacacactgtttcttcaaatgaacagcctgagaggcgaggacacggccgtgtaccactgt gcgagagaatttcgggcgccttctttggccagatacggtatggatgtc AREFRAPSLARYGMDV IGHV3-53 IGHD2-21 IGHJ6 gggttcagcgtcagtaataacttc atgacctgggtccgccaggttccagggaaggggctggagtgggtctcagtt atttatagcaatggtgaaaca atctacgcagactccgtgaagggccgattcactatgtccagagacaattccaagaacacactgtttcttcaaatgaacagcctgagaggcgaggacacggccgtgtaccactgt gcgagagaatttcgggcgccttctttggccagatacggtatggatgtc IGA2 20 210 11 9 8 13 3 7 3 0 8 9 JY8QFUQ01ACT7P 6354 productive Homsap IGHV3-11*01 F 936 92.63 201/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 3 8 8 14 8.8.14 [1.17.38.11] CTRDDRSVRGVTSDYW in-frame + ctctggattcaccttcagtgaccacttcatgagttggatccgccaggctccagggaaggggctggagtgggtttcatacattagtggcagtggtagtataatatattacgcagactctgtgaggggccgattcaccatctccagggacaacgccaagaattccctctatctgcaaatggacagcctgagagacgaggacacggccgtgtatttttgtacgagagatgatcgctcggttcggggagtcacctcagactactggggccagggaaccctggtcaccgtctcctccgcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [291] t109>c,Y37>H(+ - -)|a113>t,Y38>F(- + -)|c120>t|a172>g,S58>G(+ + -)|t174>c,S58>G(+ + -)|c191>t,T64>I(- - -)|c192>a,T64>I(- - -)|c198>t|a215>g,K72>R(+ + +)|c255>t|a258>c|g261>c|a274>g,N92>D(+ + -)|c287>a,A96>D(- - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)|g313>a,A105>T(- - -)| t109>c,Y37>H(+ - -)|a113>t,Y38>F(- + -)| c120>t| a172>g,S58>G(+ + -)|t174>c,S58>G(+ + -)|c191>t,T64>I(- - -)|c192>a,T64>I(- - -)| c198>t|a215>g,K72>R(+ + +)|c255>t|a258>c|g261>c|a274>g,N92>D(+ + -)|c287>a,A96>D(- - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g313>a,A105>T(- - -)| 320 225 208 17 5 12 3 1 5 2 1 2 2 0 1 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 1 0 0 1 0 0 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 1 1 0 1 0 0 0 0 0 0 117 114 105 9 4 5 2 0 3 0 1 1 1 0 1 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgaccacttc atgagttggatccgccaggctccagggaaggggctggagtgggtttcatac attagtggcagtggtagtataata tattacgcagactctgtgaggggccgattcaccatctccagggacaacgccaagaattccctctatctgcaaatggacagcctgagagacgaggacacggccgtgtatttttgt acgagagatgatcgctcggttcggggagtcacctcagactac TRDDRSVRGVTSDY IGHV3-11 IGHD3-10 IGHJ4 ggattcaccttcagtgaccacttc atgagttggatccgccaggctccagggaaggggctggagtgggtttcatac attagtggcagtggtagtataata tattacgcagactctgtgaggggccgattcaccatctccagggacaacgccaagaattccctctatctgcaaatggacagcctgagagacgaggacacggccgtgtatttttgt acgagagatgatcgctcggttcggggagtcacctcagactac IGG1 16 213 10 6 5 8 5 8 5 0 5 6 JY8QFUQ01ACURH 2025 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 818 86.98 187/215 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-16*02 F 2 8 7 12 8.7.12 [1.17.38.11] CARAPGWMNPFDFW in-frame + tctccggtggctccatcaccacttactacatcagctggctccggcagcccccagggaagggactggagtggattgggtgtatctcttatggtggggacactacctacaactcctccctcaagagtcgagtcaccatatcaggacaagggtccacgcgccagttctccctgaggctgagctccgtgaccgttgcggacacggccgtgtattactgtgcgcgcgccccagggtggatgaatccctttgacttctggggccagggcactctggtcaccgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctgtccagggcttcttccccca NA IGA1 100 100 [282] t78>c|g104>c,S35>T(+ - +)|t105>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|t115>a,W39>I(+ - -)|g116>t,W39>I(+ - -)|g117>c,W39>I(+ - -)|a124>c,I42>L(+ + +)|a164>g,Y55>C(- - -)|a170>c,Y57>S(+ - -)|c174>t|a175>g,S59>G(+ + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c195>t|a197>c,N66>T(- + -)|c205>t,P69>S(+ - -)|t239>g,V80>G(- - -)|g241>c,D81>Q(+ - -)|c243>a,D81>Q(+ - -)|a244>g,T82>G(+ - -)|c245>g,T82>G(+ - -)|a251>c,K84>T(- - -)|a253>c,N85>R(+ - -)|a254>g,N85>R(+ - -)|a269>g,K90>R(+ + +)|t279>c|c287>t,A96>V(+ - +)| t78>c| g104>c,S35>T(+ - +)|t105>c,S35>T(+ - +)|g107>c,S36>T(+ - +)| t115>a,W39>I(+ - -)|g116>t,W39>I(+ - -)|g117>c,W39>I(+ - -)|a124>c,I42>L(+ + +)|a164>g,Y55>C(- - -)| a170>c,Y57>S(+ - -)|c174>t|a175>g,S59>G(+ + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c195>t| a197>c,N66>T(- + -)|c205>t,P69>S(+ - -)|t239>g,V80>G(- - -)|g241>c,D81>Q(+ - -)|c243>a,D81>Q(+ - -)|a244>g,T82>G(+ - -)|c245>g,T82>G(+ - -)|a251>c,K84>T(- - -)|a253>c,N85>R(+ - -)|a254>g,N85>R(+ - -)|a269>g,K90>R(+ + +)|t279>c|c287>t,A96>V(+ - +)| a316>c|a318>c|a320>c| 315 (320) 218 (223) 190 (192) 28 (31) 4 (7) 24 6 1 4 3 5 (8) 1 0 1 4 1 1 1 78 5 4 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 1 0 0 0 0 2 0 0 0 51 51 46 5 0 5 1 0 0 0 1 0 0 1 1 0 1 0 30 21 15 6 2 4 2 1 2 0 1 0 0 0 0 0 0 0 117 114 101 13 1 12 3 0 2 1 3 1 0 0 1 1 0 1 3 (8) 3 3 (5) 0 (3) 0 (3) 0 0 0 0 0 0 (3) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctcc ggtggctccatcaccacttactac atcagctggctccggcagcccccagggaagggactggagtggattgggtgt atctcttatggtggggacact acctacaactcctccctcaagagtcgagtcaccatatcaggacaagggtccacgcgccagttctccctgaggctgagctccgtgaccgttgcggacacggccgtgtattactgt gcgcgcgccccagggtggatgaatccctttgacttc ARAPGWMNPFDF IGHV4-59 IGHD3-16 IGHJ4 ggtggctccatcaccacttactac atcagctggctccggcagcccccagggaagggactggagtggattgggtgt atctcttatggtggggacact acctacaactcctccctcaagagtcgagtcaccatatcaggacaagggtccacgcgccagttctccctgaggctgagctccgtgaccgttgcggacacggccgtgtattactgt gcgcgcgccccagggtggatgaatccctttgacttc IGA1 27 210 13 14 5 12 8 15 1 2 17 7 JY8QFUQ01ACVQ4 11189 productive Homsap IGHV4-4*07 F (see comment) 683 80 172/215 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-9*01 F 2 10 7 16 10.7.16 [1.17.38.11] CARESTNSIGDPRHLDSW in-frame + The submitted sequence and the closest germline V-GENE allele show different CDR1-IMGT amino acid lengths (10 AA in JY8QFUQ01ACVQ4 ; 8 AA in Homsap_IGHV4-4*07), and low V-REGION identity (80,00% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tctctggtggccccatcaacacacatgacttctattggacgtggatccggcagtccgccgggaggggactggagtggctcggacgtgtctatatgaatggcattagtgaccacaatccagtcttcactagtcgtctcaccatgtccattgacacgtccaagaaccagttctccctgaggctgacctctgtgaccgccgcggactcggccctatattactgtgcgagagaaagtactaattcgattggcgacccgagacaccttgactcttggggccagggaaggttggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [300] t85>c,S29>P(+ - -)|a103>c,S35>H(+ - -)|g104>a,S35>H(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t108>c,S36>D(- - -)|a110>t,Y37>F(- + -)|c114>t|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>t,P45>S(+ - -)|a143>g,K48>R(+ + +)|a157>c,I53>L(+ + +)|t159>c,I53>L(+ + +)|g162>a|a166>g,I56>V(+ - +)|c173>t,T58>M(- - -)|c174>g,T58>M(- - -)|g176>a,S59>N(- - -)|g189>c|g191>t,S64>I(- - -)|c192>t,S64>I(- - -)|c194>g,T65>S(+ - +)|c195>t,T65>S(+ - +)|a196>g,N66>D(+ + -)|t199>c,Y67>H(+ - -)|c204>t|c207>a|t208>g,S70>V(- - -)|c209>t,S70>V(- - -)|c211>t,L71>F(+ - -)|a215>c,K72>T(- - -)|g216>t,K72>T(- - -)|a225>t|g226>c,V76>L(+ - +)|a237>c|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|a295>t,T99>S(+ - +)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| t85>c,S29>P(+ - -)|a103>c,S35>H(+ - -)|g104>a,S35>H(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t108>c,S36>D(- - -)|a110>t,Y37>F(- + -)|c114>t| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>t,P45>S(+ - -)|a143>g,K48>R(+ + +)|a157>c,I53>L(+ + +)|t159>c,I53>L(+ + +)|g162>a| a166>g,I56>V(+ - +)|c173>t,T58>M(- - -)|c174>g,T58>M(- - -)|g176>a,S59>N(- - -)|g189>c|g191>t,S64>I(- - -)|c192>t,S64>I(- - -)|c194>g,T65>S(+ - +)|c195>t,T65>S(+ - +)| a196>g,N66>D(+ + -)|t199>c,Y67>H(+ - -)|c204>t|c207>a|t208>g,S70>V(- - -)|c209>t,S70>V(- - -)|c211>t,L71>F(+ - -)|a215>c,K72>T(- - -)|g216>t,K72>T(- - -)|a225>t|g226>c,V76>L(+ - +)|a237>c|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|a295>t,T99>S(+ - +)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| 320 229 186 43 7 36 5 6 8 4 4 1 4 0 5 3 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 22 8 1 7 1 2 1 2 1 0 1 0 0 0 0 0 51 51 44 7 1 6 1 1 1 1 1 0 0 0 1 1 0 0 30 21 12 9 1 8 1 1 3 0 0 0 0 0 1 2 1 0 117 114 95 19 4 15 2 2 3 1 2 1 3 0 3 0 1 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggccccatcaacacacatgacttctat tggacgtggatccggcagtccgccgggaggggactggagtggctcggacgt gtctatatgaatggcattagt gaccacaatccagtcttcactagtcgtctcaccatgtccattgacacgtccaagaaccagttctccctgaggctgacctctgtgaccgccgcggactcggccctatattactgt gcgagagaaagtactaattcgattggcgacccgagacaccttgactct ARESTNSIGDPRHLDS IGHV4-4 IGHD3-9 IGHJ4 ggtggccccatcaacacacatgacttctat tggacgtggatccggcagtccgccgggaggggactggagtggctcggacgt gtctatatgaatggcattagt gaccacaatccagtcttcactagtcgtctcaccatgtccattgacacgtccaagaaccagttctccctgaggctgacctctgtgaccgccgcggactcggccctatattactgt gcgagagaaagtactaattcgattggcgacccgagacaccttgactct IGA1 43 216 23 20 14 25 9 18 5 2 21 15 JY8QFUQ01ACXAI 17584 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 864 88.48 192/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 15 8.8.15 [1.17.38.11] CAKDASRYYYNSYCDNW in-frame + ctctggattcacctttaccaactacgccatgagctgggttcgccaggttccagggaaggggctggagtgggtctcacttattagtgttcgtggcgatgacaccttctatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtatctgcaaatggacatcctgaaacccgaggacacggccgtttatttttgcgcgaaagatgcgtctcgatattattataacagttattgtgacaattggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [294] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|t111>c|c126>t|c134>t,A45>V(+ - +)|g163>c,A55>L(+ - +)|c164>t,A55>L(+ - +)|g173>t,G58>V(- - -)|a175>c,S59>R(- - -)|t186>c|g188>a,G63>D(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a195>c|a197>t,Y66>F(- + -)|c201>t|t246>c|a274>g,N92>D(+ + -)|g278>t,S93>I(- - -)|g284>a,R95>K(+ + +)|g286>c,A96>P(- - -)|a303>t|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)|t312>c| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|t111>c| c126>t|c134>t,A45>V(+ - +)|g163>c,A55>L(+ - +)|c164>t,A55>L(+ - +)| g173>t,G58>V(- - -)|a175>c,S59>R(- - -)|t186>c|g188>a,G63>D(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a195>c| a197>t,Y66>F(- + -)|c201>t|t246>c|a274>g,N92>D(+ + -)|g278>t,S93>I(- - -)|g284>a,R95>K(+ + +)|g286>c,A96>P(- - -)|a303>t|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)|t312>c| 320 225 200 25 8 17 2 4 5 4 2 0 3 0 3 0 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 0 1 0 0 0 0 1 0 0 0 51 51 47 4 1 3 0 0 3 0 0 0 0 0 1 0 0 0 30 24 17 7 2 5 1 2 0 1 2 0 0 0 0 0 1 0 117 114 103 11 4 7 1 1 2 2 0 0 3 0 1 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaccaactacgcc atgagctgggttcgccaggttccagggaaggggctggagtgggtctcactt attagtgttcgtggcgatgacacc ttctatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtatctgcaaatggacatcctgaaacccgaggacacggccgtttatttttgc gcgaaagatgcgtctcgatattattataacagttattgtgacaat AKDASRYYYNSYCDN IGHV3-23 IGHD3-22 IGHJ4 ggattcacctttaccaactacgcc atgagctgggttcgccaggttccagggaaggggctggagtgggtctcactt attagtgttcgtggcgatgacacc ttctatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtatctgcaaatggacatcctgaaacccgaggacacggccgtttatttttgc gcgaaagatgcgtctcgatattattataacagttattgtgacaat IGG1 25 213 15 10 9 14 6 11 5 3 10 7 JY8QFUQ01ACXLB 19877 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 890 90.7 195/215 nt 89.3 192/215 nt Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD2-15*01 F 3 8 7 15 8.7.15 [1.17.38.11] CTRELRVAAARDGVDIW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 38 of V-REGION: 'G' (1 nucleotides at position 27 to 27 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 48 of V-REGION: 'G' (1 nucleotides at position 60 to 60 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 56 of V-REGION: 'C' (1 nucleotides at position 84 to 84 in the user submitted sequence), (cause frameshift); cctctggattcaccgtcagtaccaagGttcatgagctgggtccgccaggctccagggaaGggggctggagtgggtctcagtaaCtttatcctgatggtactacacactatggagcctccgtgaggggccggttcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgtacgagagaacttcgggtggctgctgcgcgtgatggtgttgacatctggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccca NA IGA1 100 100 [294] g81>a|g107>c,S36>T(+ - +)|c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)|t165>a|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|t196>c,Y66>H(+ - -)|c201>t|c203>g,A68>G(- + -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a225>g|t262>g,Y88>D(- - -)|g303>t|c309>t|g313>a,A105>T(- - -)| g81>a|g107>c,S36>T(+ - +)|c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)| t165>a| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)| t196>c,Y66>H(+ - -)|c201>t|c203>g,A68>G(- + -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a225>g|t262>g,Y88>D(- - -)|g303>t|c309>t| g313>a,A105>T(- - -)| 320 223 202 21 6 15 2 3 4 1 2 0 1 1 3 2 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 0 0 0 0 1 0 1 1 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 21 15 6 0 6 0 1 2 0 1 0 0 0 2 0 0 0 117 114 105 9 4 5 2 0 2 1 1 0 0 0 0 1 1 1 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccgtcagtaccaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagta atttatcctgatggtactaca cactatggagcctccgtgaggggccggttcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttcgggtggctgctgcgcgtgatggtgttgacatc TRELRVAAARDGVDI IGHV3-53 IGHD2-15 IGHJ3 ggattcaccgtcagtaccaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagta atttatcctgatggtactaca cactatggagcctccgtgaggggccggttcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttcgggtggctgctgcgcgtgatggtgttgacatc IGA1 20 210 9 11 6 12 3 8 5 1 5 9 JY8QFUQ01ACXOG 15898 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 843 87.27 192/220 nt NA Homsap IGHJ6*02 F (see comment) 193 79.03 49/62 nt Homsap IGHD6-19*01 F 1 8 8 12 8.8.12 [2.17.38.11] CAGSGRDPYGINVW in-frame - Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ggcctctggattcaccttcaattcctataccatgatgtgggtccgccaggctccggggaagggactggagtgggtctcaaccattagtcctagtagtcagtacatatactatgcagactctgtggagggccgattcaccatctccagagtcgacgcccggagttcagtgtttctgcaaatgaacagcctgagagacgacgacacggctgtgtattactgtgcgggcagtggcagggatccgtacggtattaacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [287] a72>g|g104>a,S35>N(- - -)|a106>t|g107>c|g113>c,S38>T(+ - +)|a119>t,N40>M(- - -)|c120>g,N40>M(- - -)|a138>g|g147>a|t163>a,S55>T(+ - +)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>g,S63>Q(- - -)|c201>t|a210>t|a214>g,K72>E(+ - -)|a242>t,D81>V(- - -)|a244>g,N82>D(+ + -)|a250>c,K84>R(+ + +)|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c287>a,A96>D(- - -)|g291>c,E97>D(+ - +)| a72>g| g104>a,S35>N(- - -)|a106>t|g107>c|g113>c,S38>T(+ - +)| a119>t,N40>M(- - -)|c120>g,N40>M(- - -)|a138>g|g147>a|t163>a,S55>T(+ - +)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>g,S63>Q(- - -)| c201>t|a210>t|a214>g,K72>E(+ - -)|a242>t,D81>V(- - -)|a244>g,N82>D(+ + -)|a250>c,K84>R(+ + +)|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c287>a,A96>D(- - -)|g291>c,E97>D(+ - +)| a316>g,R106>G(- - -)|a318>c,R106>G(- - -)|g319>a|a320>g| 315 (320) 223 (228) 195 (196) 28 (32) 7 (9) 21 (23) 6 (8) 3 (4) 2 0 3 (4) 1 5 1 4 2 0 1 78 7 6 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 1 0 0 0 0 1 0 2 0 0 0 51 51 46 5 2 3 1 1 0 0 0 0 1 1 0 1 0 0 30 24 19 5 0 5 0 1 0 0 2 0 0 0 1 0 0 1 117 114 101 13 2 11 4 0 2 0 1 1 3 0 1 1 0 0 3 (8) 3 3 (4) 0 (4) 0 (2) 0 (2) 0 (2) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ggcctct ggattcaccttcaattcctatacc atgatgtgggtccgccaggctccggggaagggactggagtgggtctcaacc attagtcctagtagtcagtacata tactatgcagactctgtggagggccgattcaccatctccagagtcgacgcccggagttcagtgtttctgcaaatgaacagcctgagagacgacgacacggctgtgtattactgt gcgggcagtggcagggatccgtacggtattaacgtc AGSGRDPYGINV IGHV3-21 IGHD6-19 IGHJ6 ggattcaccttcaattcctatacc atgatgtgggtccgccaggctccggggaagggactggagtgggtctcaacc attagtcctagtagtcagtacata tactatgcagactctgtggagggccgattcaccatctccagagtcgacgcccggagttcagtgtttctgcaaatgaacagcctgagagacgacgacacggctgtgtattactgt gcgggcagtggcagggatccgtacggtattaacgtc IGA1 27 213 10 17 5 12 5 15 4 2 14 7 JY8QFUQ01ACXS7 2349 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 810 86.18 187/217 nt 85.71 186/217 nt Homsap IGHJ2*01 F (see comment) 130 71.7 38/53 nt Homsap IGHD3-10*01 F 2 8 8 14 8.8.14 [1.17.38.11] CVKEDHDSGLRVLLEW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 and Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 51 of V-REGION: 'AG' (2 nucleotides at position 67 to 68 in the user submitted sequence), (cause frameshift); ctctggattcacgtttggcatctatgccatgagttgggtccgccaggctccagggaggggcgtggaAGgtgggtcgcaagcatgggtaatagtgctggcagtacatactacgcaggctccgtgaagggtcgcttcaacatctccagagacaattccaagaaaaccctgtatcttcaaatggacagcctgagagtcgacgacacggccagatattactgtgtgaaagaagaccatgattcgggcctcagggtcctccttgagtggggccggggaaccctggtgactgtctccgcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccctaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 71 95 [292, 294] c87>g|a103>g,S35>G(+ + -)|g107>t,S36>I(- - -)|c120>t|a143>g,K48>R(+ + +)|g147>c|c148>g,L50>V(+ - +)|t160>g,S54>A(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t165>c,A55>S(- + -)|t168>g,I56>M(+ + -)|a169>g,S57>G(+ + -)|g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|g185>c,G62>A(- + -)|t189>c|c192>t|a206>g,D69>G(- - -)|c222>t|g225>c|c230>a,T77>N(- + -)|c255>a,N85>K(+ - -)|g258>c|g267>t|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)|c314>t,A105>V(+ - +)| c87>g|a103>g,S35>G(+ + -)|g107>t,S36>I(- - -)| c120>t|a143>g,K48>R(+ + +)|g147>c|c148>g,L50>V(+ - +)|t160>g,S54>A(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t165>c,A55>S(- + -)| t168>g,I56>M(+ + -)|a169>g,S57>G(+ + -)|g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|g185>c,G62>A(- + -)|t189>c|c192>t| a206>g,D69>G(- - -)|c222>t|g225>c|c230>a,T77>N(- + -)|c255>a,N85>K(+ - -)|g258>c|g267>t|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)| c314>t,A105>V(+ - +)| 320 225 194 31 9 22 5 4 5 2 0 2 0 0 5 3 2 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 1 0 0 0 0 0 0 0 0 1 1 0 51 51 43 8 2 6 1 1 1 1 0 0 0 0 1 2 0 1 30 24 17 7 2 5 1 2 1 1 0 0 0 0 1 0 0 1 117 114 102 12 4 8 2 1 2 0 0 2 0 0 3 0 1 1 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgtttggcatctatgcc atgagttgggtccgccaggctccagggaggggcgtggagtgggtcgcaagc atgggtaatagtgctggcagtaca tactacgcaggctccgtgaagggtcgcttcaacatctccagagacaattccaagaaaaccctgtatcttcaaatggacagcctgagagtcgacgacacggccagatattactgt gtgaaagaagaccatgattcgggcctcagggtcctccttgag VKEDHDSGLRVLLE IGHV3-23 IGHD3-10 IGHJ2 ggattcacgtttggcatctatgcc atgagttgggtccgccaggctccagggaggggcgtggagtgggtcgcaagc atgggtaatagtgctggcagtaca tactacgcaggctccgtgaagggtcgcttcaacatctccagagacaattccaagaaaaccctgtatcttcaaatggacagcctgagagtcgacgacacggccagatattactgt gtgaaagaagaccatgattcgggcctcagggtcctccttgag IGA2 30 213 15 15 8 20 7 10 6 3 14 7 JY8QFUQ01ACXSL 21802 productive (see comment) Homsap IGHV4-34*10 F 809 86.51 186/215 nt 85.58 184/215 nt Homsap IGHJ4*02 F 240 100 48/48 nt Homsap IGHD4-23*01 ORF 2 8 7 16 8.7.16 [1.17.38.11] CARNTNYGGNSGDYFDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 13 to 13 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 48 of V-REGION: 'AA' (2 nucleotides at position 60 to 61 in the user submitted sequence), (cause frameshift); tgtctggtgagtGccttcactaattactactggagctggatccgccagtcccccaggaaAAgggtctggagtggcttggggaggtccatcatagtggacgcaccgactacaacccgtccctcaagagtcgaatcaccatgtcgttagacacgtccgaaaatcagttctccctgaagttgacttctttgaccgccgcggacacggcagtatattattgtgcgagaaataccaactatggtggcaattccggggactactttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [298] c75>g|a77>c,Y26>S(+ - -)|g83>a,G28>E(- - -)|g104>c,S35>T(+ - +)|g106>a,G36>N(- - -)|g107>a,G36>N(- - -)|c133>t,P45>S(+ - -)|a138>c|g139>a,G47>R(- - -)|a147>t|a157>c,I53>L(+ + +)|a165>g|a166>g,I56>V(+ - +)|a169>c,N57>H(- - -)|a190>c,S64>R(- - -)|a196>g,N66>D(+ + -)|a237>g|g238>t,V80>L(+ - +)|a250>g,K84>E(+ - -)|g252>a,K84>E(+ - -)|c255>t|a263>c,Y88>S(+ - -)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g280>t,V94>L(+ - +)|c300>a|g303>a|c309>t| c75>g|a77>c,Y26>S(+ - -)| g83>a,G28>E(- - -)|g104>c,S35>T(+ - +)|g106>a,G36>N(- - -)|g107>a,G36>N(- - -)| c133>t,P45>S(+ - -)|a138>c|g139>a,G47>R(- - -)|a147>t|a157>c,I53>L(+ + +)|a165>g| a166>g,I56>V(+ - +)|a169>c,N57>H(- - -)|a190>c,S64>R(- - -)| a196>g,N66>D(+ + -)|a237>g|g238>t,V80>L(+ - +)|a250>g,K84>E(+ - -)|g252>a,K84>E(+ - -)|c255>t|a263>c,Y88>S(+ - -)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g280>t,V94>L(+ - +)|c300>a|g303>a|c309>t| t319>a| 318 (320) 221 (223) 192 (193) 29 (30) 10 (11) 19 5 6 5 0 6 1 1 0 (1) 2 1 2 0 78 5 3 2 1 1 0 0 0 0 1 0 0 0 0 1 0 0 36 24 20 4 0 4 0 3 0 0 0 0 0 0 1 0 0 0 51 51 45 6 3 3 1 1 1 0 2 0 1 0 0 0 0 0 30 21 18 3 0 3 1 0 0 0 2 0 0 0 0 0 0 0 117 114 100 14 6 8 3 2 4 0 1 1 0 0 1 0 2 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 (1) 0 0 0 0 aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,319-320(CDR3)| tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,319-320(CDR3)| agct,8-11(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|tacc,262-265(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tgtct ggtgagtccttcactaattactac tggagctggatccgccagtcccccaggaagggtctggagtggcttggggag gtccatcatagtggacgcacc gactacaacccgtccctcaagagtcgaatcaccatgtcgttagacacgtccgaaaatcagttctccctgaagttgacttctttgaccgccgcggacacggcagtatattattgt gcgagaaataccaactatggtggcaattccggggactactttgactac ARNTNYGGNSGDYFDY IGHV4-34 IGHD4-23 IGHJ4 ggtgagtccttcactaattactac tggagctggatccgccagtcccccaggaagggtctggagtggcttggggag gtccatcatagtggacgcacc gactacaacccgtccctcaagagtcgaatcaccatgtcgttagacacgtccgaaaatcagttctccctgaagttgacttctttgaccgccgcggacacggcagtatattattgt gcgagaaataccaactatggtggcaattccggggactactttgactac IGG1 27 210 16 11 11 16 5 11 9 0 11 7 JY8QFUQ01AD4KE 13549 productive (see comment) Homsap IGHV4-61*02 F 893 89.59 198/221 nt 88.69 196/221 nt Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F 150 79.17 38/48 nt Homsap IGHD3-10*02 F 3 10 7 16 10.7.16 [2.16.38.11] CAREHSRTMGGARELDSW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 59 of V-REGION: 'T' (1 nucleotides at position 96 to 96 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 65 in the user submitted sequence), (cause frameshift) gtctctggtggccccatcagcagcagcacttactactggacttggatccggcaggccgccgggagggactggagtggatcggacgtgtttatactTagtgggagcaccaactacaatccctccctcaagagtcgaatcaccatatcactagacacgtccaagaaccagatcttcctgaagttgaggtctgtgaccgcctctgacacggccgtatattactgtgcgagagaacatagcaggacgatgggcggggcgagagagctagactcctggggcccgggcactctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [301] t85>c,S29>P(+ - -)|t102>c|g103>a,G35>S(+ + -)|t105>c,G35>S(+ + -)|g107>c,S36>T(+ - +)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|c133>g,P45>A(- - -)|t159>c|g162>a|a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|c174>t|c204>t|g226>a,V76>I(+ - +)|g238>c,V80>L(+ - +)|t259>a,F87>I(+ - -)|c263>t,S88>F(- - -)|c271>t|c276>g,S92>R(- - -)|g289>t,A97>S(- + -)|a291>t,A97>S(- + -)|g303>a| t85>c,S29>P(+ - -)|t102>c|g103>a,G35>S(+ + -)|t105>c,G35>S(+ + -)|g107>c,S36>T(+ - +)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|c133>g,P45>A(- - -)|t159>c|g162>a| a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|c174>t| c204>t|g226>a,V76>I(+ - +)|g238>c,V80>L(+ - +)|t259>a,F87>I(+ - -)|c263>t,S88>F(- - -)|c271>t|c276>g,S92>R(- - -)|g289>t,A97>S(- + -)|a291>t,A97>S(- + -)|g303>a| 320 229 206 23 7 16 1 4 6 4 0 0 1 1 3 2 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 25 5 1 4 0 1 0 3 0 0 0 0 1 0 0 0 51 50 45 5 2 3 0 1 1 1 0 0 0 0 1 1 0 0 30 21 18 3 1 2 1 0 2 0 0 0 0 0 0 0 0 0 117 114 104 10 3 7 0 2 3 0 0 0 1 1 1 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct ggtggccccatcagcagcagcacttactac tggacttggatccggcaggccgccgggagggactggagtggatcggacgt gtttatactagtgggagcacc aactacaatccctccctcaagagtcgaatcaccatatcactagacacgtccaagaaccagatcttcctgaagttgaggtctgtgaccgcctctgacacggccgtatattactgt gcgagagaacatagcaggacgatgggcggggcgagagagctagactcc AREHSRTMGGARELDS IGHV4-61 IGHD3-10 IGHJ4 ggtggccccatcagcagcagcacttactac tggacttggatccggcaggccgccgggagggactggagtggatcggacgt gtttatactagtgggagcacc aactacaatccctccctcaagagtcgaatcaccatatcactagacacgtccaagaaccagatcttcctgaagttgaggtctgtgaccgcctctgacacggccgtatattactgt gcgagagaacatagcaggacgatgggcggggcgagagagctagactcc IGA1 23 215 15 8 10 16 5 7 5 2 10 6 JY8QFUQ01AD5QJ 8902 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 927 92.17 200/217 nt NA Homsap IGHJ6*02 F 274 93.55 58/62 nt Homsap IGHD3-3*01 F 3 8 8 15 8.8.15 [1.17.38.11] CAKQTIFDYYYYGLDVW in-frame - ctctggattcatgtttagtagctttcccatggcctgggtccgccaggctccagggaaggggctggagtgggtctctagtattagtggtaggggtggtaacacatacttcgcagactccgtgaagggccggttcaacatctccagagacaattccaagaacacgatgtatttgcaaatgaacagcctgagagccgaggacacggccttatattactgtgcgaagcagacgattttcgactactactactacggtttggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [293, 292] c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|c105>t|a110>t,Y37>F(- + -)|g112>c,A38>P(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a162>t|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t177>g,S59>R(- - -)|g191>a,S64>N(- - -)|a200>t,Y67>F(- + -)|c230>a,T77>N(- + -)|c259>a,L87>M(+ + -)|c265>t|g301>t,V101>L(+ - +)| c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|c105>t|a110>t,Y37>F(- + -)|g112>c,A38>P(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a162>t|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| t177>g,S59>R(- - -)|g191>a,S64>N(- - -)| a200>t,Y67>F(- + -)|c230>a,T77>N(- + -)|c259>a,L87>M(+ + -)|c265>t|g301>t,V101>L(+ - +)| a318>g|g319>c| 317 (320) 222 (225) 205 (206) 17 (19) 3 (5) 14 1 (2) 2 3 0 0 2 3 0 2 (3) 2 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 0 2 0 0 0 1 0 1 1 0 0 51 51 46 5 1 4 1 1 0 0 0 0 1 0 1 1 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 117 114 109 5 1 4 0 0 1 0 0 2 1 0 0 0 1 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatgtttagtagctttccc atggcctgggtccgccaggctccagggaaggggctggagtgggtctctagt attagtggtaggggtggtaacaca tacttcgcagactccgtgaagggccggttcaacatctccagagacaattccaagaacacgatgtatttgcaaatgaacagcctgagagccgaggacacggccttatattactgt gcgaagcagacgattttcgactactactactacggtttggacgtc AKQTIFDYYYYGLDV IGHV3-23 IGHD3-3 IGHJ6 ggattcatgtttagtagctttccc atggcctgggtccgccaggctccagggaaggggctggagtgggtctctagt attagtggtaggggtggtaacaca tacttcgcagactccgtgaagggccggttcaacatctccagagacaattccaagaacacgatgtatttgcaaatgaacagcctgagagccgaggacacggccttatattactgt gcgaagcagacgattttcgactactactactacggtttggacgtc IGA2 17 213 6 11 5 12 1 5 2 1 8 6 JY8QFUQ01AD5UE 13185 productive Homsap IGHV3-74*03 F 914 91.28 199/218 nt NA Homsap IGHJ5*02 F 147 76.47 39/51 nt Homsap IGHD6-19*01 F 1 8 8 14 8.8.14 [1.17.38.11] CAKVDSDGWHGTIDRW in-frame + cctctggattcaccttcagtaattactggatgtactgggtccgccaagttccagggaaggggctggtgtgggtcgcccgtattaataacgatgggagtagcaaaacttacgcagactccgtgaggggccgattcaccatctccagagacaacgccaagaacacactgtttctgcaaatgaacagtctgagaggcgaggacacggcttcatattattgtgcaaaagtggatagcgatggctggcacggaacaatagaccgctggggccagggagtcctggtcaccgtctccgcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [291] g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|c118>t,H40>Y(+ - -)|c134>t,A45>V(+ - +)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)|g173>a,S58>N(- - -)|t174>c,S58>N(- - -)|c194>a,T65>K(- - -)|g198>t|g204>a|a215>g,K72>R(+ + +)|g258>a|a263>t,Y88>F(- + -)|c287>g,A96>G(- + -)|g301>t,V101>S(- - -)|t302>c,V101>S(- - -)|g303>a,V101>S(- - -)|c309>t|g317>a,R106>K(+ + +)| g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| c118>t,H40>Y(+ - -)|c134>t,A45>V(+ - +)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)| g173>a,S58>N(- - -)|t174>c,S58>N(- - -)|c194>a,T65>K(- - -)| g198>t|g204>a|a215>g,K72>R(+ + +)|g258>a|a263>t,Y88>F(- + -)|c287>g,A96>G(- + -)|g301>t,V101>S(- - -)|t302>c,V101>S(- - -)|g303>a,V101>S(- - -)|c309>t| g317>a,R106>K(+ + +)|a320>t| 319 (320) 225 (226) 205 20 (21) 4 (5) 16 1 6 4 2 1 1 1 (2) 0 0 1 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 51 51 47 4 0 4 0 0 2 0 1 0 0 0 0 0 0 1 30 24 21 3 0 3 0 1 0 1 0 1 0 0 0 0 0 0 117 114 104 10 4 6 1 3 1 1 0 0 1 0 0 1 2 0 7 (8) 7 6 1 (2) 0 (1) 1 0 1 0 0 0 0 0 (1) 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtaattactgg atgtactgggtccgccaagttccagggaaggggctggtgtgggtcgcccgt attaataacgatgggagtagcaaa acttacgcagactccgtgaggggccgattcaccatctccagagacaacgccaagaacacactgtttctgcaaatgaacagtctgagaggcgaggacacggcttcatattattgt gcaaaagtggatagcgatggctggcacggaacaatagaccgc AKVDSDGWHGTIDR IGHV3-74 IGHD6-19 IGHJ5 ggattcaccttcagtaattactgg atgtactgggtccgccaagttccagggaaggggctggtgtgggtcgcccgt attaataacgatgggagtagcaaa acttacgcagactccgtgaggggccgattcaccatctccagagacaacgccaagaacacactgtttctgcaaatgaacagtctgagaggcgaggacacggcttcatattattgt gcaaaagtggatagcgatggctggcacggaacaatagaccgc IGA1 19 213 12 7 9 13 3 6 4 0 10 5 JY8QFUQ01AD74T 7077 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 891 90.32 196/217 nt NA Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD5-24*01 ORF 3 8 8 16 8.8.16 [1.17.38.11] CVRYDYSRLHRSNYFGPW in-frame + ctctggattcaccttcatcagttatggcatgagttgggtccgccagtttccagggaaggggctggagtgggtctcatctattagtgattatggtaataccgcattctacgcagactccgtgaagggccggttcaccatctccagagacaattccaacaacacgctgtttctgcaaatgagcagcctgagagccgaggacacggccgtttattattgtgtgagatatgactactcacgcctacacagatccaactacttcggcccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [296] t90>c|g104>t,S35>I(- - -)|c108>t|c113>g,A38>G(- + -)|c120>t|g133>t,A45>F(+ - -)|c134>t,A45>F(+ - -)|g163>t,A55>S(- + -)|g173>a,G58>D(- - -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|g187>a,G63>N(- - -)|g188>a,G63>N(- - -)|g191>c,S64>T(+ - +)|a193>g,T65>A(- - -)|a197>t,Y66>F(- + -)|g252>c,K84>N(+ - -)|a263>t,Y88>F(- + -)|a275>g,N92>S(- - -)|a303>t|c309>t|c314>t,A105>V(+ - +)| t90>c|g104>t,S35>I(- - -)|c108>t|c113>g,A38>G(- + -)| c120>t|g133>t,A45>F(+ - -)|c134>t,A45>F(+ - -)|g163>t,A55>S(- + -)| g173>a,G58>D(- - -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|g187>a,G63>N(- - -)|g188>a,G63>N(- - -)|g191>c,S64>T(+ - +)|a193>g,T65>A(- - -)| a197>t,Y66>F(- + -)|g252>c,K84>N(+ - -)|a263>t,Y88>F(- + -)|a275>g,N92>S(- - -)|a303>t|c309>t| c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)|g319>t| 316 (320) 221 (225) 199 (201) 22 (24) 5 (6) 17 (18) 2 (3) 4 5 1 0 0 4 0 2 1 3 (4) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 0 1 1 0 0 0 0 0 1 1 0 51 51 47 4 1 3 0 0 2 0 0 0 0 0 0 0 2 0 30 24 17 7 0 7 1 4 0 0 0 0 1 0 1 0 0 0 117 114 108 6 2 4 1 0 1 0 0 0 3 0 1 0 0 0 4 (8) 4 3 (5) 1 (3) 0 (1) 1 (2) 0 (1) 0 1 0 0 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcatcagttatggc atgagttgggtccgccagtttccagggaaggggctggagtgggtctcatct attagtgattatggtaataccgca ttctacgcagactccgtgaagggccggttcaccatctccagagacaattccaacaacacgctgtttctgcaaatgagcagcctgagagccgaggacacggccgtttattattgt gtgagatatgactactcacgcctacacagatccaactacttcggcccc VRYDYSRLHRSNYFGP IGHV3-23 IGHD5-24 IGHJ5 ggattcaccttcatcagttatggc atgagttgggtccgccagtttccagggaaggggctggagtgggtctcatct attagtgattatggtaataccgca ttctacgcagactccgtgaagggccggttcaccatctccagagacaattccaacaacacgctgtttctgcaaatgagcagcctgagagccgaggacacggccgtttattattgt gtgagatatgactactcacgcctacacagatccaactacttcggcccc IGA2 21 213 11 10 8 14 3 7 3 2 7 9 JY8QFUQ01AD79A 9211 productive Homsap IGHV3-49*03 F, or Homsap IGHV3-49*05 F 849 87 194/223 nt NA Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD4-23*01 ORF 3 8 10 14 8.10.14 [1.17.38.11] CSRRSTLVTGYVFDKW in-frame + Other possibilities: Homsap_IGHJ1*01 (highest number of consecutive identical nucleotides) ctctggaatcaactttcgtgatcatgccatgagctggttccgccaggttccagggaaggggctggagtgggtaggtttcattagaagttctcaatacggtggagatacagaatacgccgcgtctgtggaaggcagattcaccatctcaagagacgattccaaaagcatcgcctatttggatatgaatagcctgaaaatcgacgacacagccctatattactgttcccggaggtcgacgttggtgactggatacgtctttgacaagtggggccagggcaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [297] t75>c|t82>a,F28>I(+ - -)|c86>a,T29>N(- + -)|g103>c,G35>R(- - -)|t109>c,Y37>H(+ - -)|t114>c|c134>t,A45>V(+ - +)|c174>t|a175>t,K59>S(- - -)|a176>c,K59>S(- - -)|a177>t,K59>S(- - -)|g178>c,A60>Q(- - -)|c179>a,A60>Q(- - -)|t180>a,A60>Q(- - -)|t183>c|g189>a|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|a192>t,T64>D(- + -)|a214>g,K72>E(+ - -)|t243>c|c265>t|c268>g,Q90>D(+ - -)|a270>t,Q90>D(+ - -)|c276>t|c287>t,T96>I(- - -)|g291>c,E97>D(+ - +)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| t75>c| t82>a,F28>I(+ - -)|c86>a,T29>N(- + -)|g103>c,G35>R(- - -)|t109>c,Y37>H(+ - -)|t114>c| c134>t,A45>V(+ - +)| c174>t|a175>t,K59>S(- - -)|a176>c,K59>S(- - -)|a177>t,K59>S(- - -)|g178>c,A60>Q(- - -)|c179>a,A60>Q(- - -)|t180>a,A60>Q(- - -)|t183>c|g189>a|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|a192>t,T64>D(- + -)| a214>g,K72>E(+ - -)|t243>c|c265>t|c268>g,Q90>D(+ - -)|a270>t,Q90>D(+ - -)|c276>t|c287>t,T96>I(- - -)|g291>c,E97>D(+ - +)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| a313>t,T105>S(+ - +)|t315>c,T105>S(+ - +)|a316>c|a318>g|g319>a|a320>g| 312 (320) 223 (231) 194 (196) 29 (35) 8 (12) 21 (23) 2 (4) 2 (3) 5 5 (6) 1 (2) 3 4 (5) 2 4 1 0 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 0 0 2 0 1 0 1 1 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 30 18 12 3 9 1 1 1 1 1 2 3 1 1 0 0 0 117 114 104 10 3 7 1 1 3 1 0 0 1 0 2 1 0 0 0 (8) 0 0 (2) 0 (6) 0 (4) 0 (2) 0 (2) 0 (1) 0 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,158-159(FR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,181-182(CDR2)|aa,192-193(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|ta,69-70(FR1)|tt,75-76(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,124-125(FR2)|ta,158-159(FR2)|tt,162-163(FR2)|tt,167-168(CDR2)|tt,180-181(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agct,72-75(FR1)|agct,118-121(FR2)|ggtt,122-125(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggta,156-159(FR2)|ggtt,160-163(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|ggca,220-223(FR3)|agca,253-256(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,72-75(FR1)|tgct,111-114(CDR1)|agct,118-121(FR2)|agct,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| ctct ggaatcaactttcgtgatcatgcc atgagctggttccgccaggttccagggaaggggctggagtgggtaggtttc attagaagttctcaatacggtggagataca gaatacgccgcgtctgtggaaggcagattcaccatctcaagagacgattccaaaagcatcgcctatttggatatgaatagcctgaaaatcgacgacacagccctatattactgt tcccggaggtcgacgttggtgactggatacgtctttgacaag SRRSTLVTGYVFDK IGHV3-49 IGHD4-23 IGHJ4 ggaatcaactttcgtgatcatgcc atgagctggttccgccaggttccagggaaggggctggagtgggtaggtttc attagaagttctcaatacggtggagataca gaatacgccgcgtctgtggaaggcagattcaccatctcaagagacgattccaaaagcatcgcctatttggatatgaatagcctgaaaatcgacgacacagccctatattactgt tcccggaggtcgacgttggtgactggatacgtctttgacaag IGG1 28 219 13 15 7 15 6 13 3 4 8 13 JY8QFUQ01ADCX5 9835 productive Homsap IGHV4-61*02 F 893 89.59 198/221 nt NA Homsap IGHJ6*02 F (see comment) 202 80.65 50/62 nt Homsap IGHD2-15*01 F 2 10 7 20 10.7.20 [1.17.38.11] CARDPRVICSGGSCRSSAMDVW in-frame + Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) tctccggtggctccatgagaagtggaagcaactactgggcctggatccggcagcccgccgggaagggactggagtggcttgggcgtatatatgccactgggagcagcaaccacaacccctccctgcagggtcgagtcaccatgtcagtagacacgtccaaaaaccagttctccctgaggctgatctctgtgaccgccgcagacacggccgtgtacttctgtgcgagagaccccagggttatttgtagtggtggtagctgccgctcttctgcaatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [313] t78>c|c90>g,I30>M(+ + -)|c93>a,S31>R(- - -)|t105>a|t108>c|t109>a,Y37>N(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a157>c,I53>L(+ + +)|c168>a|a172>g,T58>A(- - -)|g176>c,S59>T(+ - +)|c194>g,T65>S(+ - +)|t199>c,Y67>H(+ - -)|c213>g|a214>c,K72>Q(+ - -)|a220>g,S74>G(+ + -)|a234>g,I78>M(+ + -)|g252>a|a269>g,K90>R(+ + +)|g275>t,S92>I(- - -)|t306>c|a308>t,Y103>F(- + -)| t78>c| c90>g,I30>M(+ + -)|c93>a,S31>R(- - -)|t105>a|t108>c|t109>a,Y37>N(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a157>c,I53>L(+ + +)| c168>a|a172>g,T58>A(- - -)|g176>c,S59>T(+ - +)|c194>g,T65>S(+ - +)| t199>c,Y67>H(+ - -)|c213>g|a214>c,K72>Q(+ - -)|a220>g,S74>G(+ + -)|a234>g,I78>M(+ + -)|g252>a|a269>g,K90>R(+ + +)|g275>t,S92>I(- - -)|t306>c|a308>t,Y103>F(- + -)| 320 229 206 23 7 16 5 1 0 4 2 2 1 2 2 3 1 0 78 5 4 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 30 25 5 2 3 0 0 0 1 0 1 0 2 0 1 0 0 51 51 48 3 0 3 1 0 0 0 1 0 0 0 1 0 0 0 30 21 17 4 1 3 1 0 0 0 0 1 0 0 1 1 0 0 117 114 104 10 3 7 3 1 0 2 1 0 1 0 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctcc ggtggctccatgagaagtggaagcaactac tgggcctggatccggcagcccgccgggaagggactggagtggcttgggcgt atatatgccactgggagcagc aaccacaacccctccctgcagggtcgagtcaccatgtcagtagacacgtccaaaaaccagttctccctgaggctgatctctgtgaccgccgcagacacggccgtgtacttctgt gcgagagaccccagggttatttgtagtggtggtagctgccgctcttctgcaatggacgtc ARDPRVICSGGSCRSSAMDV IGHV4-61 IGHD2-15 IGHJ6 ggtggctccatgagaagtggaagcaactac tgggcctggatccggcagcccgccgggaagggactggagtggcttgggcgt atatatgccactgggagcagc aaccacaacccctccctgcagggtcgagtcaccatgtcagtagacacgtccaaaaaccagttctccctgaggctgatctctgtgaccgccgcagacacggccgtgtacttctgt gcgagagaccccagggttatttgtagtggtggtagctgccgctcttctgcaatggacgtc IGG2 22 216 9 13 1 9 8 13 3 3 10 6 JY8QFUQ01ADDDA 13877 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 998 96.28 207/215 nt 95.81 206/215 nt Homsap IGHJ5*02 F 192 86.27 44/51 nt Homsap IGHD3-22*01 F 2 8 7 15 8.7.15 [1.17.38.11] CARADNYDSLGLKFDPW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 39 of V-REGION: 'C' (1 nucleotides at position 30 to 30 in the user submitted sequence), (cause frameshift); tctctggtgactccatcagtagtaactacCtggaactggatccggcagcccccagggaagggactggagtggattgggtatatctatcacagtgggagcaccagctacaacccctccctcaggagtcgagtcaccatatcattagacacgtccaagaaccagttctccctgaaactgagctctgtgaccgctgcggacacggccgtgtattactgtgcgagggcggataattatgatagtcttgggctgaagttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [292] g83>a,G28>D(- - -)|t109>a,Y37>N(- - -)|g119>a,S40>N(- - -)|t172>c,Y58>H(+ - -)|a197>g,N66>S(- - -)|a215>g,K72>R(+ + +)|g238>t,V80>L(+ - +)|g270>a| g83>a,G28>D(- - -)|t109>a,Y37>N(- - -)| g119>a,S40>N(- - -)| t172>c,Y58>H(+ - -)| a197>g,N66>S(- - -)|a215>g,K72>R(+ + +)|g238>t,V80>L(+ - +)|g270>a| a318>g|a320>c| 317 (320) 220 (223) 212 (213) 8 (10) 1 (3) 7 2 (3) 3 0 1 0 (1) 0 0 1 0 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 1 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 21 20 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 117 114 110 4 1 3 2 1 0 0 0 0 0 0 0 0 1 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatcagtagtaactac tggaactggatccggcagcccccagggaagggactggagtggattgggtat atctatcacagtgggagcacc agctacaacccctccctcaggagtcgagtcaccatatcattagacacgtccaagaaccagttctccctgaaactgagctctgtgaccgctgcggacacggccgtgtattactgt gcgagggcggataattatgatagtcttgggctgaagttcgacccc ARADNYDSLGLKFDP IGHV4-59 IGHD3-22 IGHJ5 ggtgactccatcagtagtaactac tggaactggatccggcagcccccagggaagggactggagtggattgggtat atctatcacagtgggagcacc agctacaacccctccctcaggagtcgagtcaccatatcattagacacgtccaagaaccagttctccctgaaactgagctctgtgaccgctgcggacacggccgtgtattactgt gcgagggcggataattatgatagtcttgggctgaagttcgacccc IGA1 8 210 6 2 3 4 3 4 1 0 4 3 JY8QFUQ01ADG23 18486 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*03 F or Homsap IGHV4-39*05 F (see comment) 790 84.23 187/222 nt NA Homsap IGHJ5*02 F (see comment) 192 86.27 44/51 nt Homsap IGHD3-9*01 F 2 10 7 11 10.7.11 [2.17.38.11] CARILDGYFFDPW in-frame - Low V-REGION identity (84,23% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) gtctctggtggctccatcgacactcaaaattactactggggctggattcgccagcccccagggacgggactggagtgggttggcagtgtccgctatggcgagagcacctattacaacccgaccctcaaaagtcgactcaccatatccatagacacgtccaggagccagttatccctgagactgagttctgtcaccgccgccgacacggcagtttactactgtgcgaggatcttggatggttacttcttcgacccctggggccagggaacccacgtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [287] a91>g,S31>D(- - -)|g92>a,S31>D(- - -)|g101>c,S34>T(+ - +)|a103>c,S35>Q(- - -)|g104>a,S35>Q(- - -)|t105>a,S35>Q(- - -)|g107>a,S36>N(- - -)|c126>t|a143>c,K48>T(- - -)|g147>a|a157>g,I53>V(+ - +)|g162>c|a166>g,I56>V(+ - +)|t169>c,Y57>R(- - -)|a170>g,Y57>R(- - -)|t171>c,Y57>R(- - -)|a175>g,S59>G(+ + -)|t177>c,S59>G(+ + -)|g188>a,G63>E(- - -)|c198>t|t208>a,S70>T(+ - +)|g216>a|g226>c,V76>L(+ - +)|g238>a,V80>I(+ - +)|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|c261>a,F87>L(+ - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c276>t|g282>c|a291>c|t300>a|g303>t|t306>c| a91>g,S31>D(- - -)|g92>a,S31>D(- - -)|g101>c,S34>T(+ - +)|a103>c,S35>Q(- - -)|g104>a,S35>Q(- - -)|t105>a,S35>Q(- - -)|g107>a,S36>N(- - -)| c126>t|a143>c,K48>T(- - -)|g147>a|a157>g,I53>V(+ - +)|g162>c| a166>g,I56>V(+ - +)|t169>c,Y57>R(- - -)|a170>g,Y57>R(- - -)|t171>c,Y57>R(- - -)|a175>g,S59>G(+ + -)|t177>c,S59>G(+ + -)|g188>a,G63>E(- - -)| c198>t|t208>a,S70>T(+ - +)|g216>a|g226>c,V76>L(+ - +)|g238>a,V80>I(+ - +)|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|c261>a,F87>L(+ - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c276>t|g282>c|a291>c|t300>a|g303>t|t306>c| a318>g|c319>a|a320>t| 317 (320) 227 (230) 192 35 (38) 11 (14) 24 8 (9) 8 3 4 3 1 (2) 0 (1) 3 4 0 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 23 7 0 7 1 3 0 0 1 0 0 1 1 0 0 0 51 51 46 5 3 2 1 1 1 0 1 0 0 0 1 0 0 0 30 21 14 7 0 7 3 1 0 3 0 0 0 0 0 0 0 0 117 114 98 16 8 8 3 3 2 1 1 1 0 2 2 0 1 0 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 0 0 0 0 (1) 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct ggtggctccatcgacactcaaaattactac tggggctggattcgccagcccccagggacgggactggagtgggttggcagt gtccgctatggcgagagcacc tattacaacccgaccctcaaaagtcgactcaccatatccatagacacgtccaggagccagttatccctgagactgagttctgtcaccgccgccgacacggcagtttactactgt gcgaggatcttggatggttacttcttcgacccc ARILDGYFFDP IGHV4-39 IGHD3-9 IGHJ5 ggtggctccatcgacactcaaaattactac tggggctggattcgccagcccccagggacgggactggagtgggttggcagt gtccgctatggcgagagcacc tattacaacccgaccctcaaaagtcgactcaccatatccatagacacgtccaggagccagttatccctgagactgagttctgtcaccgccgccgacacggcagtttactactgt gcgaggatcttggatggttacttcttcgacccc IGG1 35 216 23 12 11 17 12 18 11 0 10 14 JY8QFUQ01ADGL2 14486 productive Homsap IGHV3-7*01 F 896 90.37 197/218 nt NA Homsap IGHJ3*01 F 223 94 47/50 nt Homsap IGHD1-14*01 ORF 1 8 8 14 8.8.14 [1.17.38.11] CARVIPGRGNYAFDVW in-frame - cctctggattcgactttggtagttattggatgagttgggtccgccaggctccagggaagggactggagtgggtggccagcataaagcgagatgcaagtgagaagtaccatgtggaatctgtgcagagacgattcaccatcttcagagacaacgtcaggaactcactgtatttgcagatgaacagcctgagagacgaggacacggctgtgtattactgtgcgagagttattcccggccggggaaactatgcttttgatgtctggggccgagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [291] a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|a103>g,S35>G(+ + -)|c108>t|c120>t|g147>a|a164>g,N55>S(- - -)|a173>g,Q58>R(+ - -)|g185>c,G62>A(- + -)|a195>g|t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a214>c,K72>Q(+ - -)|g220>a,G74>R(- - -)|c222>a,G74>R(- - -)|c236>t,S79>F(- - -)|c248>t,A83>V(+ - +)|a251>g,K84>R(+ + +)|c265>t|a270>g|c287>a,A96>D(- - -)| a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|a103>g,S35>G(+ + -)|c108>t| c120>t|g147>a|a164>g,N55>S(- - -)| a173>g,Q58>R(+ - -)|g185>c,G62>A(- + -)|a195>g| t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a214>c,K72>Q(+ - -)|g220>a,G74>R(- - -)|c222>a,G74>R(- - -)|c236>t,S79>F(- - -)|c248>t,A83>V(+ - +)|a251>g,K84>R(+ + +)|c265>t|a270>g|c287>a,A96>D(- - -)| a320>t| 319 (320) 225 (226) 204 21 (22) 6 (7) 15 7 2 5 1 1 4 0 (1) 0 1 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 2 0 1 0 0 1 0 0 0 0 0 0 51 51 48 3 2 1 1 1 1 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 2 0 0 0 0 0 0 0 1 0 0 0 117 114 103 11 2 9 2 1 3 1 1 3 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcgactttggtagttattgg atgagttgggtccgccaggctccagggaagggactggagtgggtggccagc ataaagcgagatgcaagtgagaag taccatgtggaatctgtgcagagacgattcaccatcttcagagacaacgtcaggaactcactgtatttgcagatgaacagcctgagagacgaggacacggctgtgtattactgt gcgagagttattcccggccggggaaactatgcttttgatgtc ARVIPGRGNYAFDV IGHV3-7 IGHD1-14 IGHJ3 ggattcgactttggtagttattgg atgagttgggtccgccaggctccagggaagggactggagtgggtggccagc ataaagcgagatgcaagtgagaag taccatgtggaatctgtgcagagacgattcaccatcttcagagacaacgtcaggaactcactgtatttgcagatgaacagcctgagagacgaggacacggctgtgtattactgt gcgagagttattcccggccggggaaactatgcttttgatgtc IGA1 21 213 15 6 7 12 8 9 4 2 10 5 JY8QFUQ01ADMJX 10613 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 818 86.98 187/215 nt 86.05 185/215 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-15*01 F 2 8 7 10 8.7.10 [1.17.38.11] CATLRGGTFDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 50 to 50 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 64 of V-REGION: 'A' (1 nucleotides at position 99 to 99 in the user submitted sequence), (cause frameshift); tctctggtgcctccatcaggagttattattggagttggatccggcagccCcccaggaaagggactggagtggattggttatattaattatgttgggggAcaccgattacaacccctccctcaagagtcgagtctccatgtcagcagccacgtccaagaaccaggtcttcctgcagctgacctctgtgaccgctgcggacaccgcctattatttctgtgcgacactgagagggggtactttcgactactggggccggggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggcacagcggccctggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 79 94 [279] g83>c,G28>A(- + -)|t105>g,S35>R(- - -)|c111>t|c114>t|c120>t|g141>a|g162>t|c168>t|t169>a,Y57>N(- - -)|c174>t|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|a190>g,S64>G(+ + -)|a196>g,N66>D(+ + -)|c198>t,N66>D(+ + -)|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|t239>c,V80>A(+ - +)|a242>c,D81>A(- - -)|t259>g,F87>V(+ - -)|c263>t,S88>F(- - -)|a268>c,K90>Q(+ - -)|g275>c,S92>T(+ - +)|g297>c|g301>t,V101>Y(- - -)|t302>a,V101>Y(- - -)|g303>t,V101>Y(- - -)|a308>t,Y103>F(- + -)| g83>c,G28>A(- + -)|t105>g,S35>R(- - -)|c111>t|c114>t| c120>t|g141>a|g162>t| c168>t|t169>a,Y57>N(- - -)|c174>t|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|a190>g,S64>G(+ + -)| a196>g,N66>D(+ + -)|c198>t,N66>D(+ + -)|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|t239>c,V80>A(+ - +)|a242>c,D81>A(- - -)|t259>g,F87>V(+ - -)|c263>t,S88>F(- - -)|a268>c,K90>Q(+ - -)|g275>c,S92>T(+ - +)|g297>c|g301>t,V101>Y(- - -)|t302>a,V101>Y(- - -)|g303>t,V101>Y(- - -)|a308>t,Y103>F(- + -)| g317>c,R106>T(- - -)|g319>c|a320>t| 316 (320) 219 (223) 191 (192) 28 (31) 8 (10) 20 (21) 4 1 7 1 2 0 2 (3) 2 3 (5) 0 4 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 0 2 0 0 0 0 0 1 0 0 1 51 51 48 3 3 0 0 1 1 0 0 0 0 0 0 0 1 0 30 21 15 6 2 4 2 0 2 0 0 0 0 1 0 0 1 0 117 114 99 15 1 14 2 0 2 1 2 0 2 1 2 0 2 1 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 0 0 0 0 0 0 (1) 0 0 (2) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgcctccatcaggagttattat tggagttggatccggcagcccccaggaaagggactggagtggattggttat attaattatgttgggggcacc gattacaacccctccctcaagagtcgagtctccatgtcagcagccacgtccaagaaccaggtcttcctgcagctgacctctgtgaccgctgcggacaccgcctattatttctgt gcgacactgagagggggtactttcgactac ATLRGGTFDY IGHV4-59 IGHD2-15 IGHJ4 ggtgcctccatcaggagttattat tggagttggatccggcagcccccaggaaagggactggagtggattggttat attaattatgttgggggcacc gattacaacccctccctcaagagtcgagtctccatgtcagcagccacgtccaagaaccaggtcttcctgcagctgacctctgtgaccgctgcggacaccgcctattatttctgt gcgacactgagagggggtactttcgactac IGG1 28 210 13 15 8 15 5 13 4 4 14 6 JY8QFUQ01ADN3R 7224 productive Homsap IGHV2-5*02 F, or Homsap IGHV2-5*05 F or Homsap IGHV2-5*06 F or Homsap IGHV2-5*09 F 839 86.88 192/221 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD6-6*01 F 3 10 7 11 10.7.11 [1.17.38.11] CAHFGPQEHFDFW in-frame + tctctggattctcactcagcaccaatggaatgggtgtgggttggatccgccagcccccgggagaggccccagactggctcgctctcatttattgggatgatgataagcgataccggccatccctagagagtagactcaccatcaccaaggacatctccacaaaccaggtggtccttagaatgaccgacatgggccctgcagacacagccacatatttctgtgcacactttggaccacaggagcacttcgacttttggggccagggaatcctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [285] g81>a|t102>c|g104>a,S35>N(- - -)|g109>a,V37>M(+ - -)|c120>t|t129>c|a138>g|a142>g,K48>E(+ - -)|t149>c,L50>P(- - -)|g150>a,L50>P(- - -)|g153>c,E51>D(+ - +)|t159>c|a162>t|c198>a|a202>c,S68>R(- - -)|c204>g,S68>R(- - -)|t210>c|g213>a|a214>g,K72>E(+ - -)|c222>t|g225>a|c245>t,T82>I(- - -)|a251>c,K84>T(- - -)|c269>g,T90>R(- - -)|a277>g,N93>D(+ + -)|a284>g,D95>G(- - -)|t290>c,V97>A(+ - +)|g291>a,V97>A(+ - +)|a308>t,Y103>F(- + -)| g81>a|t102>c|g104>a,S35>N(- - -)|g109>a,V37>M(+ - -)| c120>t|t129>c|a138>g|a142>g,K48>E(+ - -)|t149>c,L50>P(- - -)|g150>a,L50>P(- - -)|g153>c,E51>D(+ - +)|t159>c|a162>t| c198>a|a202>c,S68>R(- - -)|c204>g,S68>R(- - -)|t210>c|g213>a|a214>g,K72>E(+ - -)|c222>t|g225>a|c245>t,T82>I(- - -)|a251>c,K84>T(- - -)|c269>g,T90>R(- - -)|a277>g,N93>D(+ + -)|a284>g,D95>G(- - -)|t290>c,V97>A(+ - +)|g291>a,V97>A(+ - +)|a308>t,Y103>F(- + -)| a319>t,R107>F(- - -)|g320>t,R107>F(- - -)|a321>t,R107>F(- - -)|c322>g| 318 (322) 227 (231) 198 29 (33) 12 (13) 17 (20) 5 7 3 6 2 1 2 (4) 0 1 2 (3) 0 (1) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 2 2 0 3 0 1 0 0 0 0 0 0 0 0 51 51 42 9 5 4 2 1 1 3 0 0 1 0 1 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 98 16 5 11 3 3 2 2 2 1 1 0 0 2 0 0 6 (10) 6 6 0 (4) 0 (1) 0 (3) 0 0 0 0 0 0 0 (2) 0 0 0 (1) 0 (1) 0 aa,13-14(FR1)|aa,40-41(FR1)|ta,102-103(CDR1)|aa,141-142(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,238-239(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,267-268(FR3)|aa,270-271(FR3)|aa,277-278(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,10-11(FR1)|tt,73-74(FR1)|tt,82-83(CDR1)|ta,102-103(CDR1)|tt,158-159(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,80-83(CDR1)|ggct,118-121(FR2)|ggct,155-158(FR2)|agca,220-223(FR3)|ggct,224-227(FR3)| aacc,41-44(FR1)|agcc,131-134(FR2)|agcc,202-205(FR3)|aacc,253-256(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)| tctct ggattctcactcagcaccaatggaatgggt gtgggttggatccgccagcccccgggagaggccccagactggctcgctctc atttattgggatgatgataag cgataccggccatccctagagagtagactcaccatcaccaaggacatctccacaaaccaggtggtccttagaatgaccgacatgggccctgcagacacagccacatatttctgt gcacactttggaccacaggagcacttcgacttt AHFGPQEHFDF IGHV2-5 IGHD6-6 IGHJ4 ggattctcactcagcaccaatggaatgggt gtgggttggatccgccagcccccgggagaggccccagactggctcgctctc atttattgggatgatgataag cgataccggccatccctagagagtagactcaccatcaccaaggacatctccacaaaccaggtggtccttagaatgaccgacatgggccctgcagacacagccacatatttctgt gcacactttggaccacaggagcacttcgacttt IGA1 29 216 21 8 10 14 11 15 10 2 15 2 JY8QFUQ01ADNKP 13690 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 801 85.71 186/217 nt 85.25 185/217 nt Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD3-16*02 F 2 8 8 17 8.8.17 [1.17.38.11] CVKDRDFLWGTYRLQLEHW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 39 of V-REGION: 'A' (1 nucleotides at position 30 to 30 in the user submitted sequence), (cause frameshift); ctctggattcagatttagcagctatgccaAtgagctgggtccgccagactccagaaaaggggcttgaatgggtctcaggcatcaatgataacggtcgaagcataaactacgcgggctccgtgaagggccggttcaccatctccagagacaattccaagagcacgttgtatctgcagatggatagcctgagccccgaggactcgggcatatattattgtgtgaaagatcgtgatttcctttggggcacttatcgtcttcaacttgaacattggggccagggagccctggtcagcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [300] c86>g,T29>R(- - -)|c87>a,T29>R(- - -)|g133>a,A45>T(- - -)|g140>a,G47>E(- - -)|g141>a,G47>E(- - -)|g150>t|g153>a|c164>g,A55>G(- + -)|t165>c,A55>G(- + -)|t168>c|g170>a,S57>N(- - -)|g173>a,G58>D(- - -)|g176>a,S59>N(- - -)|t177>c,S59>N(- - -)|g187>c,G63>R(- - -)|t189>a,G63>R(- - -)|c194>t,T65>I(- - -)|t196>a,Y66>N(- - -)|a204>g|a206>g,D69>G(- - -)|a254>g,N85>S(- - -)|c259>t|a270>g|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|a295>t,T99>S(+ - +)|c299>g,A100>G(- + -)|g301>a,V101>I(+ - +)|c309>t|c314>t,A105>V(+ - +)| c86>g,T29>R(- - -)|c87>a,T29>R(- - -)| g133>a,A45>T(- - -)|g140>a,G47>E(- - -)|g141>a,G47>E(- - -)|g150>t|g153>a|c164>g,A55>G(- + -)|t165>c,A55>G(- + -)| t168>c|g170>a,S57>N(- - -)|g173>a,G58>D(- - -)|g176>a,S59>N(- - -)|t177>c,S59>N(- - -)|g187>c,G63>R(- - -)|t189>a,G63>R(- - -)|c194>t,T65>I(- - -)| t196>a,Y66>N(- - -)|a204>g|a206>g,D69>G(- - -)|a254>g,N85>S(- - -)|c259>t|a270>g|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|a295>t,T99>S(+ - +)|c299>g,A100>G(- + -)|g301>a,V101>I(+ - +)|c309>t| c314>t,A105>V(+ - +)| 320 225 193 32 7 25 5 8 5 3 1 1 1 2 2 3 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 1 0 0 0 1 0 0 51 51 44 7 2 5 0 4 0 1 0 0 0 0 0 1 1 0 30 24 16 8 1 7 0 3 1 2 0 0 0 1 1 0 0 0 117 114 100 14 4 10 5 1 3 0 1 0 1 1 1 1 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagatttagcagctatgcc atgagctgggtccgccagactccagaaaaggggcttgaatgggtctcaggc atcaatgataacggtcgaagcata aactacgcgggctccgtgaagggccggttcaccatctccagagacaattccaagagcacgttgtatctgcagatggatagcctgagccccgaggactcgggcatatattattgt gtgaaagatcgtgatttcctttggggcacttatcgtcttcaacttgaacat VKDRDFLWGTYRLQLEH IGHV3-23 IGHD3-16 IGHJ4 ggattcagatttagcagctatgcc atgagctgggtccgccagactccagaaaaggggcttgaatgggtctcaggc atcaatgataacggtcgaagcata aactacgcgggctccgtgaagggccggttcaccatctccagagacaattccaagagcacgttgtatctgcagatggatagcctgagccccgaggactcgggcatatattattgt gtgaaagatcgtgatttcctttggggcacttatcgtcttcaacttgaacat IGA1 31 213 20 11 12 19 8 12 6 1 15 9 JY8QFUQ01ADOO5 20155 productive (see comment) Homsap IGHV4-4*07 F (see comment) 688 80.09 173/216 nt 79.63 172/216 nt Homsap IGHJ4*01 F, or Homsap IGHJ4*02 F or Homsap IGHJ4*03 F 159 81.25 39/48 nt Homsap IGHD2-8*01 F 2 10 7 16 10.7.16 [2.17.38.11] CARECTNEAGGPRHLDSW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (80,09% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR1-IMGT at codon 29 of V-REGION: 'C' (1 nucleotides at position 14 to 14 in the user submitted sequence), (cause frameshift); gtctctggtggccCccatcaatagtagtgactactattggacttggatccggcagcccgccgggaggggactggaatgggtcgggcgtgtcttcatgaatggccttaccgactacaatccatccttcggcagccgtctcaccatgtccattgacatgtcgaagaaccaattctccctgaagttgacctctgtgaccggcgctgacacggccctttattactgtgcgagagaatgtactaatgaggctggcggaccgagacacctggactcctggggccacggaactctggtcaccgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [302] t85>c,S29>P(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t108>c,S36>D(- - -)|c114>t|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|a143>g,K48>R(+ + +)|g153>a|a157>g,I53>V(+ - +)|t159>c,I53>V(+ - +)|a166>g,I56>V(+ - +)|a170>t,Y57>F(- + -)|t171>c,Y57>F(- + -)|c173>t,T58>M(- - -)|c174>g,T58>M(- - -)|g176>a,S59>N(- - -)|g189>c|a190>c,S64>L(- - -)|g191>t,S64>L(- - -)|c192>t,S64>L(- - -)|a196>g,N66>D(+ + -)|c204>t|c207>a|c211>t,L71>F(+ - -)|a214>g,K72>G(- - -)|a215>g,K72>G(- - -)|g216>c,K72>G(- - -)|t222>c|a225>t|g226>c,V76>L(+ - +)|a237>c|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|c245>t,T82>M(- - -)|c249>g|g258>a|c271>t|g275>c,S92>T(+ - +)|c287>g,A96>G(- + -)|g291>t|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)| t85>c,S29>P(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t108>c,S36>D(- - -)|c114>t| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|a143>g,K48>R(+ + +)|g153>a|a157>g,I53>V(+ - +)|t159>c,I53>V(+ - +)| a166>g,I56>V(+ - +)|a170>t,Y57>F(- + -)|t171>c,Y57>F(- + -)|c173>t,T58>M(- - -)|c174>g,T58>M(- - -)|g176>a,S59>N(- - -)|g189>c|a190>c,S64>L(- - -)|g191>t,S64>L(- - -)|c192>t,S64>L(- - -)| a196>g,N66>D(+ + -)|c204>t|c207>a|c211>t,L71>F(+ - -)|a214>g,K72>G(- - -)|a215>g,K72>G(- - -)|g216>c,K72>G(- - -)|t222>c|a225>t|g226>c,V76>L(+ - +)|a237>c|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|c245>t,T82>M(- - -)|c249>g|g258>a|c271>t|g275>c,S92>T(+ - +)|c287>g,A96>G(- + -)|g291>t|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)| 320 230 187 43 12 31 7 5 8 5 2 1 3 0 6 3 3 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 25 5 1 4 1 1 1 2 0 0 0 0 0 0 0 0 51 51 45 6 1 5 2 1 1 1 0 0 0 0 1 0 0 0 30 21 11 10 1 9 1 1 2 1 1 0 1 0 1 1 1 0 117 114 92 22 9 13 3 2 4 1 1 1 2 0 4 2 2 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct ggtggccccatcaatagtagtgactactat tggacttggatccggcagcccgccgggaggggactggaatgggtcgggcgt gtcttcatgaatggccttacc gactacaatccatccttcggcagccgtctcaccatgtccattgacatgtcgaagaaccaattctccctgaagttgacctctgtgaccggcgctgacacggccctttattactgt gcgagagaatgtactaatgaggctggcggaccgagacacctggactcc ARECTNEAGGPRHLDS IGHV4-4 IGHD2-8 IGHJ4 ggtggccccatcaatagtagtgactactat tggacttggatccggcagcccgccgggaggggactggaatgggtcgggcgt gtcttcatgaatggccttacc gactacaatccatccttcggcagccgtctcaccatgtccattgacatgtcgaagaaccaattctccctgaagttgacctctgtgaccggcgctgacacggccctttattactgt gcgagagaatgtactaatgaggctggcggaccgagacacctggactcc IGA1 43 216 25 18 13 26 12 17 10 2 18 13 JY8QFUQ01ADRD7 4180 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*02 F or Homsap IGHV4-59*07 F or Homsap IGHV4-61*01 F or Homsap IGHV4-61*08 F 845 90.29 186/206 nt 89.81 185/206 nt Homsap IGHJ4*02 F (see comment) 168 83.33 40/48 nt Homsap IGHD1-1*01 F 3 5 7 12 5.7.12 [1.17.38.11] CARHPKSNVEGDSW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT, from codon 30 of V-REGION: 9 nucleotides (from position 15 in the user submitted sequence), (do not cause frameshift) tctctggtgactcccacttctggagctggatccggcagcccccgggaaagggcctggagtggattggttatgtctataacagtgggaccaccaactacaacccctccctcaggagtcgagtcaccatgtctatagacacgtccaagaagcagatctctctgaggttgaactctgtgaccgctgcggacacggccgtgtattactgtgcgagacacccgaagtccaacgtagagggtgactcctggggccagggaaccctggtcaccgtctccgcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctgtccagggcttcttccccca NA IGA1 100 100 [273] g83>a,G28>D(- - -)|t109>c,Y37>H(+ - -)|a113>t,Y38>F(- + -)|a138>g|g141>a|a147>c|g162>t|a166>g,I56>V(+ - +)|t172>a,Y58>N(- - -)|g191>c,S64>T(+ - +)|a215>g,K72>R(+ + +)|a234>g,I78>M(+ + -)|a237>t|g238>a,V80>I(+ - +)|c255>g,N85>K(+ - -)|t259>a,F87>I(+ - -)|c264>t|a269>g,K90>R(+ + +)|c271>t|g275>a,S92>N(- - -)| g83>a,G28>D(- - -)|t109>c,Y37>H(+ - -)|a113>t,Y38>F(- + -)| a138>g|g141>a|a147>c|g162>t| a166>g,I56>V(+ - +)|t172>a,Y58>N(- - -)|g191>c,S64>T(+ - +)| a215>g,K72>R(+ + +)|a234>g,I78>M(+ + -)|a237>t|g238>a,V80>I(+ - +)|c255>g,N85>K(+ - -)|t259>a,F87>I(+ - -)|c264>t|a269>g,K90>R(+ + +)|c271>t|g275>a,S92>N(- - -)| g319>c| 318 (320) 212 (214) 192 (193) 20 (21) 7 (8) 13 5 4 2 1 1 0 2 2 1 (2) 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 15 12 3 0 3 0 1 0 1 0 0 1 0 0 0 0 0 51 51 47 4 4 0 1 1 0 0 1 0 0 0 0 0 1 0 30 21 18 3 0 3 1 0 0 0 0 0 0 1 1 0 0 0 117 114 104 10 3 7 3 2 2 0 0 0 1 1 0 1 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactcccacttc tggagctggatccggcagcccccgggaaagggcctggagtggattggttat gtctataacagtgggaccacc aactacaacccctccctcaggagtcgagtcaccatgtctatagacacgtccaagaagcagatctctctgaggttgaactctgtgaccgctgcggacacggccgtgtattactgt gcgagacacccgaagtccaacgtagagggtgactcc ARHPKSNVEGDS IGHV4-59 IGHD1-1 IGHJ4 ggtgactcccacttc tggagctggatccggcagcccccgggaaagggcctggagtggattggttat gtctataacagtgggaccacc aactacaacccctccctcaggagtcgagtcaccatgtctatagacacgtccaagaagcagatctctctgaggttgaactctgtgaccgctgcggacacggccgtgtattactgt gcgagacacccgaagtccaacgtagagggtgactcc IGA1 20 201 12 8 6 9 6 11 7 0 7 6 JY8QFUQ01ADTQJ 18018 productive Homsap IGHV3-7*01 F 887 89.45 195/218 nt NA Homsap IGHJ1*01 F (see comment) 161 78.85 41/52 nt Homsap IGHD6-19*01 F 1 8 8 10 8.8.10 [1.17.38.11] CVVSSGWLFHYW in-frame + Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) cctctggattcacctctcctagatactggatgaattgggtccgccaggcttccgggaaggggctggagtgggtggccaacataaagcaagacggaagtgaggaaaactttgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaattcaatgtctctacaaatgaacagcctgagagtcgaggacacggctgtatattattgcgttgttagcagtggctggttgttccactactggggccagggctcccgggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggcctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [280] t89>c,F30>S(- - -)|a103>c,S35>P(+ - -)|g104>c,S35>P(+ - -)|c108>a,S36>R(- - -)|t111>c|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|c136>t,P46>S(+ - -)|a138>c,P46>S(+ - -)|t177>c|a193>g,K65>E(+ - -)|t196>a,Y66>N(- - -)|a200>t,Y67>F(- + -)|a225>g|a245>g,N82>S(- - -)|c255>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|c287>t,A96>V(+ - +)|g303>a|c309>t| t89>c,F30>S(- - -)|a103>c,S35>P(+ - -)|g104>c,S35>P(+ - -)|c108>a,S36>R(- - -)|t111>c| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|c136>t,P46>S(+ - -)|a138>c,P46>S(+ - -)| t177>c|a193>g,K65>E(+ - -)| t196>a,Y66>N(- - -)|a200>t,Y67>F(- + -)|a225>g|a245>g,N82>S(- - -)|c255>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|c287>t,A96>V(+ - +)|g303>a|c309>t|t312>c| c314>t,A105>V(+ - +)|g315>t,A105>V(+ - +)|a316>g,R106>V(- - -)|g317>t,R106>V(- - -)|a318>t,R106>V(- - -)|g319>a|a320>g| 311 (320) 217 (226) 195 (196) 22 (30) 7 (10) 15 (20) 3 (5) 3 (4) 5 (6) 3 (4) 3 2 1 (2) 1 1 0 0 (2) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 0 0 2 1 1 0 0 1 0 0 0 51 51 47 4 0 4 0 1 2 0 1 0 0 0 0 0 0 0 30 24 22 2 1 1 1 0 0 1 0 0 0 0 0 0 0 0 116 (117) 114 102 11 5 6 2 2 3 0 1 1 1 1 0 0 0 0 0 (8) 0 0 (1) 0 (7) 0 (2) 0 (5) 0 (2) 0 (1) 0 (1) 0 (0) 0 0 0 (1) 0 0 0 0 (2) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctctcctagatactgg atgaattgggtccgccaggcttccgggaaggggctggagtgggtggccaac ataaagcaagacggaagtgaggaa aactttgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaattcaatgtctctacaaatgaacagcctgagagtcgaggacacggctgtatattattgc gttgttagcagtggctggttgttccactac VVSSGWLFHY IGHV3-7 IGHD6-19 IGHJ1 ggattcacctctcctagatactgg atgaattgggtccgccaggcttccgggaaggggctggagtgggtggccaac ataaagcaagacggaagtgaggaa aactttgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaattcaatgtctctacaaatgaacagcctgagagtcgaggacacggctgtatattattgc gttgttagcagtggctggttgttccactac IGG2 22 213 14 8 8 11 6 11 5 2 10 5 JY8QFUQ01ADV0A 18274 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 974 93.67 207/221 nt 93.21 206/221 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-19*01 F 1 10 7 13 10.7.13 [1.17.38.11] CARHGSGWLQELDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 39 of V-REGION: 'G' (1 nucleotides at position 37 to 37 in the user submitted sequence), (cause frameshift); tctctggtggctccatcatcagagacagtgcctactGggggctggatccgccagcccccagggaaggggctggagtggcttgggagcatctattatagtgggagtacctactacaatccctccctcaagagtcgagtcaccatatccgtagacacgtccaagaagcagttctccctgaagctgagctctgtgaccgccgcagacacggctgtatattactgtgcgagacatggcagtggctggctccaggaacttgactactggggccagggaaccctggtcaccgtctccacagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA1 71 100 [292] g92>t,S31>I(- - -)|t102>a,S34>R(- - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|t105>c,S35>D(- - -)|t109>g,Y37>A(- - -)|a110>c,Y37>A(- - -)|a157>c,I53>L(+ + +)|t165>c|c192>t|c204>t|g207>c|c255>g,N85>K(+ - -)|g303>a| g92>t,S31>I(- - -)|t102>a,S34>R(- - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|t105>c,S35>D(- - -)|t109>g,Y37>A(- - -)|a110>c,Y37>A(- - -)| a157>c,I53>L(+ + +)|t165>c| c192>t| c204>t|g207>c|c255>g,N85>K(+ - -)|g303>a| 320 229 215 14 5 9 1 2 2 2 2 0 0 1 1 1 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 23 7 0 7 1 1 0 1 1 0 0 1 0 0 1 1 51 51 49 2 1 1 0 0 0 1 1 0 0 0 0 0 0 0 30 21 20 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 117 114 110 4 3 1 0 1 1 0 0 0 0 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcatcagagacagtgcctac tggggctggatccgccagcccccagggaaggggctggagtggcttgggagc atctattatagtgggagtacc tactacaatccctccctcaagagtcgagtcaccatatccgtagacacgtccaagaagcagttctccctgaagctgagctctgtgaccgccgcagacacggctgtatattactgt gcgagacatggcagtggctggctccaggaacttgactac ARHGSGWLQELDY IGHV4-39 IGHD6-19 IGHJ4 ggtggctccatcatcagagacagtgcctac tggggctggatccgccagcccccagggaaggggctggagtggcttgggagc atctattatagtgggagtacc tactacaatccctccctcaagagtcgagtcaccatatccgtagacacgtccaagaagcagttctccctgaagctgagctctgtgaccgccgcagacacggctgtatattactgt gcgagacatggcagtggctggctccaggaacttgactac IGA1 14 216 7 7 4 7 3 7 4 1 2 7 JY8QFUQ01ADWZ0 9468 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 927 92.17 200/217 nt NA Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F (see comment) 177 85.42 41/48 nt Homsap IGHD4-23*01 ORF 2 8 8 17 8.8.17 [1.17.38.11] CARVGGTEEFAGMLELDYW in-frame + Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) gtctggattcagtttcagtagctttggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatgatctggtatgatggaactaataaatattatgcagactccgtgaagggccgattcaccatctcgagagacaattccaagaacacactgtttctgctcatggacagcctgacagccgacgacacggctgtctattactgtgcgagagtcgggggaaccgaggagttcgctggtatgttggaacttgactactggggccaggggaccctggtcaccgtctccgcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [299] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a110>t,Y37>F(- + -)|g163>a,V55>M(+ - -)|t165>g,V55>M(+ - -)|a168>c|g188>c,S63>T(+ - +)|c198>t|c237>g|g258>a|a263>t,Y88>F(- + -)|a269>t,Q90>L(- - -)|a270>c,Q90>L(- - -)|a274>g,N92>D(+ + -)|g284>c,R95>T(- - -)|g291>c,E97>D(+ - +)|g303>c| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a110>t,Y37>F(- + -)| g163>a,V55>M(+ - -)|t165>g,V55>M(+ - -)| a168>c|g188>c,S63>T(+ - +)| c198>t|c237>g|g258>a|a263>t,Y88>F(- + -)|a269>t,Q90>L(- - -)|a270>c,Q90>L(- - -)|a274>g,N92>D(+ + -)|g284>c,R95>T(- - -)|g291>c,E97>D(+ - +)|g303>c| a320>t| 319 (320) 224 (225) 207 17 (18) 5 (6) 12 1 2 2 0 2 0 3 (4) 0 4 2 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 1 0 0 0 1 0 0 1 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 30 24 22 2 1 1 0 0 0 0 1 0 0 0 1 0 0 0 117 114 104 10 4 6 1 1 1 0 1 0 2 0 3 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcagtttcagtagctttggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatg atctggtatgatggaactaataaa tattatgcagactccgtgaagggccgattcaccatctcgagagacaattccaagaacacactgtttctgctcatggacagcctgacagccgacgacacggctgtctattactgt gcgagagtcgggggaaccgaggagttcgctggtatgttggaacttgactac ARVGGTEEFAGMLELDY IGHV3-33 IGHD4-23 IGHJ4 ggattcagtttcagtagctttggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatg atctggtatgatggaactaataaa tattatgcagactccgtgaagggccgattcaccatctcgagagacaattccaagaacacactgtttctgctcatggacagcctgacagccgacgacacggctgtctattactgt gcgagagtcgggggaaccgaggagttcgctggtatgttggaacttgactac IGA1 17 213 5 12 4 10 1 7 4 1 8 4 JY8QFUQ01ADZFS 14177 productive Homsap IGHV3-7*01 F 923 91.74 200/218 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-3*02 F 2 8 8 14 8.8.14 [1.17.38.11] CARGSYWHYDTFGDYW in-frame + cctctggatttacttttaacaactattggatgacctgggttcgccaggctccagggaaggggctggaatgggtggccaacataaagcaacatggaggtgaaacgtactatgcggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgtgcgagagggtcgtattggcattatgataccttcggcgactattggggcccgggagtcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [292] c84>t|c87>t|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|c126>t|g153>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)|t203>c,V68>A(+ - +)|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| c84>t|c87>t|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|c126>t|g153>a| g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)| t203>c,V68>A(+ - +)|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| a320>g| 319 (320) 225 (226) 207 18 (19) 6 (7) 12 3 (4) 4 3 2 2 0 0 0 3 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 2 2 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 0 1 0 0 0 30 24 19 5 1 4 2 1 0 0 1 0 0 0 1 0 0 0 117 114 109 5 1 4 1 0 0 1 1 0 0 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggatttacttttaacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccaac ataaagcaacatggaggtgaaacg tactatgcggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat ARGSYWHYDTFGDY IGHV3-7 IGHD3-3 IGHJ4 ggatttacttttaacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccaac ataaagcaacatggaggtgaaacg tactatgcggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat IGG1 18 213 12 6 7 11 5 7 3 3 5 7 JY8QFUQ01AE2LT 10298 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 864 88.94 193/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD1-26*01 F 1 8 8 16 8.8.16 [1.17.38.11] CAKVMWGVTGPIDPFDYW in-frame - ctctggattcacgttcaatgactatgccatgagctgggtccgccaggcgccagataaggggctggagtgggtctcgactgtgagtagtaggggtgataccacacactacgcagacttcgtgaagggccggctcaccatctccagagacaattccaggaacacactgtatctgcaaatgaacagcctgacagccgaggacacggccatatattactgtgcgaaagttatgtggggagttacaggtccaattgatccctttgactactggggccagggaaccctggtcaccgtctcctccgcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [296] c87>g|t90>c|g104>a,S35>N(- - -)|c105>t,S35>N(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t135>g|g140>a,G47>D(- - -)|g141>t,G47>D(- - -)|a162>g|g163>a,A55>T(- - -)|a166>g,I56>V(+ - +)|t168>g,I56>V(+ - +)|g172>a,G58>S(+ + -)|t177>g,S59>R(- - -)|g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)|t196>c,Y66>H(+ - -)|c209>t,S70>F(- - -)|t226>c,F76>L(+ - -)|a251>g,K84>R(+ + +)|g258>a|g284>c,R95>T(- - -)|g301>a,V101>I(+ - +)| c87>g|t90>c|g104>a,S35>N(- - -)|c105>t,S35>N(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| t135>g|g140>a,G47>D(- - -)|g141>t,G47>D(- - -)|a162>g|g163>a,A55>T(- - -)| a166>g,I56>V(+ - +)|t168>g,I56>V(+ - +)|g172>a,G58>S(+ + -)|t177>g,S59>R(- - -)|g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)| t196>c,Y66>H(+ - -)|c209>t,S70>F(- - -)|t226>c,F76>L(+ - -)|a251>g,K84>R(+ + +)|g258>a|g284>c,R95>T(- - -)|g301>a,V101>I(+ - +)| a320>t| 319 (320) 224 (225) 200 24 (25) 5 (6) 19 4 8 2 3 0 0 0 (1) 0 2 1 1 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 2 4 1 2 1 1 0 0 0 0 0 1 0 0 51 51 46 5 2 3 1 2 0 0 0 0 0 0 0 0 1 1 30 24 18 6 0 6 1 2 0 0 0 0 0 0 1 0 0 2 117 114 107 7 1 6 1 2 1 2 0 0 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgttcaatgactatgcc atgagctgggtccgccaggcgccagataaggggctggagtgggtctcgact gtgagtagtaggggtgataccaca cactacgcagacttcgtgaagggccggctcaccatctccagagacaattccaggaacacactgtatctgcaaatgaacagcctgacagccgaggacacggccatatattactgt gcgaaagttatgtggggagttacaggtccaattgatccctttgactac AKVMWGVTGPIDPFDY IGHV3-23 IGHD1-26 IGHJ4 ggattcacgttcaatgactatgcc atgagctgggtccgccaggcgccagataaggggctggagtgggtctcgact gtgagtagtaggggtgataccaca cactacgcagacttcgtgaagggccggctcaccatctccagagacaattccaggaacacactgtatctgcaaatgaacagcctgacagccgaggacacggccatatattactgt gcgaaagttatgtggggagttacaggtccaattgatccctttgactac IGA1 24 213 17 7 10 14 7 10 3 2 9 10 JY8QFUQ01AE3XC 2727 productive (see comment) Homsap IGHV4-39*02 F 957 91.93 205/223 nt 91.48 204/223 nt Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD1-26*01 F 3 10 7 13 10.7.13 [2.16.38.11] CARRYSWNYERFDPW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 45 of V-REGION: 1 nucleotides (from position 59 in the user submitted sequence), (cause frameshift) ctgtctctggtgactccatcagtactaataattactactgggcctggatccgccagccccagggagggggctggagtggattgggaatatctattatagcgggaccacctactacaatccgtccctcaagagtcgagtcaccatgtccgtagacacgtccaagaaccacttctccctgaggttgagttctgtgaccgccgcagacacggctctctattactgcgcgcggcgatatagttggaactacgaaaggttcgacccctggggccagggaaccctggtcatcgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [294] g83>a,G28>D(- - -)|c93>t|g101>c,S34>T(+ - +)|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|g119>c,G40>A(- + -)|a143>g,K48>R(+ + +)|g164>a,S55>N(- - -)|t177>c|g191>c,S64>T(+ - +)|c204>t|a234>g,I78>M(+ + -)|a269>g,K90>R(+ + +)|c271>t|c276>t|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t312>c| g83>a,G28>D(- - -)|c93>t|g101>c,S34>T(+ - +)|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)| g119>c,G40>A(- + -)|a143>g,K48>R(+ + +)|g164>a,S55>N(- - -)| t177>c|g191>c,S64>T(+ - +)| c204>t|a234>g,I78>M(+ + -)|a269>g,K90>R(+ + +)|c271>t|c276>t|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t312>c| a316>c|a318>g|g319>c|a320>g| 315 (320) 226 (231) 208 (209) 18 (22) 6 (10) 12 3 (5) 4 4 2 0 (1) 0 0 0 5 (6) 0 0 0 78 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 25 5 1 4 0 3 1 0 0 0 0 0 1 0 0 0 51 50 47 3 0 3 1 1 0 0 0 0 0 0 1 0 0 0 30 21 19 2 1 1 0 0 0 1 0 0 0 0 1 0 0 0 117 114 106 8 4 4 2 0 3 1 0 0 0 0 2 0 0 0 3 (8) 3 3 (4) 0 (4) 0 (4) 0 0 (2) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctgtctct ggtgactccatcagtactaataattactac tgggcctggatccgccagccccagggagggggctggagtggattgggaat atctattatagcgggaccacc tactacaatccgtccctcaagagtcgagtcaccatgtccgtagacacgtccaagaaccacttctccctgaggttgagttctgtgaccgccgcagacacggctctctattactgc gcgcggcgatatagttggaactacgaaaggttcgacccc ARRYSWNYERFDP IGHV4-39 IGHD1-26 IGHJ5 ggtgactccatcagtactaataattactac tgggcctggatccgccagccccagggagggggctggagtggattgggaat atctattatagcgggaccacc tactacaatccgtccctcaagagtcgagtcaccatgtccgtagacacgtccaagaaccacttctccctgaggttgagttctgtgaccgccgcagacacggctctctattactgc gcgcggcgatatagttggaactacgaaaggttcgacccc IGG1 18 215 13 5 8 13 5 5 4 2 7 5 JY8QFUQ01AE4GX 19826 productive (see comment) Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 899 89.29 200/224 nt 88.84 199/224 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-6*01 F 2 8 10 12 8.10.12 [1.17.38.11] CTITVRPDDYHDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 61 to 61 in the user submitted sequence), (cause frameshift); tctctggagtcactttcactaacgtgtggatgagttgggtccggcaggctccagggaaggGggccggagtgggttggccgtattaaaagggagactgagggggggacaatagactacgctgcacccgtgacagcaagattcaccatgtcaaaagatgattcaaaaaacacactatatctgcaaatgaacaacctgaaaatggaggacacagccgtgtattactgtaccataacagtccgaccggatgattaccatgactactggggccagggaaccctggtgaccgtctccccagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [292] c74>t|t82>g,F28>V(+ - -)|g104>c,S35>T(+ - +)|c110>t,A37>V(+ - +)|c111>g,A37>V(+ - +)|c120>t|c129>g|t149>c,L50>P(- - -)|c174>g,S58>R(- - -)|a175>g,K59>E(+ - -)|a177>g,K59>E(+ - -)|t183>g,D61>E(+ - +)|t186>g|c194>t,T65>I(- - -)|a215>c,K72>T(- - -)|g221>c,G74>A(- + -)|c222>a,G74>A(- + -)|c234>g,I78>M(+ + -)|g239>a,R80>K(+ + +)|g258>a|g261>a|g278>a,S93>N(- - -)|c287>t,T96>M(- - -)|c288>g,T96>M(- - -)| c74>t| t82>g,F28>V(+ - -)|g104>c,S35>T(+ - +)|c110>t,A37>V(+ - +)|c111>g,A37>V(+ - +)| c120>t|c129>g|t149>c,L50>P(- - -)| c174>g,S58>R(- - -)|a175>g,K59>E(+ - -)|a177>g,K59>E(+ - -)|t183>g,D61>E(+ - +)|t186>g|c194>t,T65>I(- - -)| a215>c,K72>T(- - -)|g221>c,G74>A(- + -)|c222>a,G74>A(- + -)|c234>g,I78>M(+ + -)|g239>a,R80>K(+ + +)|g258>a|g261>a|g278>a,S93>N(- - -)|c287>t,T96>M(- - -)|c288>g,T96>M(- - -)| c317>t,T106>I(- - -)|g319>a|a320>c| 316 (320) 228 (232) 204 (205) 24 (27) 6 (8) 18 (19) 2 4 (5) 5 (6) 1 1 (2) 1 0 0 2 5 0 3 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 1 0 0 0 0 0 1 1 0 1 51 51 48 3 2 1 0 0 1 1 0 0 0 0 0 1 0 0 30 30 24 6 1 5 2 0 1 0 0 0 0 0 0 1 0 2 117 114 104 10 2 8 0 4 1 0 1 1 0 0 1 2 0 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| tctct ggagtcactttcactaacgtgtgg atgagttgggtccggcaggctccagggaaggggccggagtgggttggccgt attaaaagggagactgagggggggacaata gactacgctgcacccgtgacagcaagattcaccatgtcaaaagatgattcaaaaaacacactatatctgcaaatgaacaacctgaaaatggaggacacagccgtgtattactgt accataacagtccgaccggatgattaccatgactac TITVRPDDYHDY IGHV3-15 IGHD6-6 IGHJ4 ggagtcactttcactaacgtgtgg atgagttgggtccggcaggctccagggaaggggccggagtgggttggccgt attaaaagggagactgagggggggacaata gactacgctgcacccgtgacagcaagattcaccatgtcaaaagatgattcaaaaaacacactatatctgcaaatgaacaacctgaaaatggaggacacagccgtgtattactgt accataacagtccgaccggatgattaccatgactac IGA2 23 219 11 12 8 16 3 7 4 1 9 9 JY8QFUQ01AE6UZ 7050 productive Homsap IGHV3-30*02 F, or Homsap IGHV3-30-5*02 F 882 89.86 195/217 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD6-19*01 F 1 8 8 14 8.8.14 [1.17.38.11] CAKEEFDTGWTQPGDW in-frame + ctctggattcatcttcagtgactatggcatgcactgggtccgccaggctccaggcgaggggctggattgggtggcatttatacgatatgatggaaatgagatacactatccagactccgtgaggggccgattcaccatctccagagacaattccaagaacaccctatatctagaaatgaacaatgtgagacctgaggacacggctgtgtattactgtgcgaaggaggaatttgacactggctggacacaaccgggcgactggggccagggaacgctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [290] g75>c|c86>t,T29>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a142>g,K48>E(+ - -)|g153>t,E51>D(+ - +)|g171>a|g188>a,S63>N(- - -)|a190>g,N64>E(+ - -)|t192>g,N64>E(+ - -)|a194>t,K65>I(- + -)|t196>c,Y66>H(+ - -)|g202>c,A68>P(- - -)|a215>g,K72>R(+ + +)|g258>c|g261>a|g267>a|c268>g,Q90>E(+ + -)|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|c280>g,L94>V(+ - +)|g286>c,A96>P(- - -)|a318>g| g75>c| c86>t,T29>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| a142>g,K48>E(+ - -)|g153>t,E51>D(+ - +)| g171>a|g188>a,S63>N(- - -)|a190>g,N64>E(+ - -)|t192>g,N64>E(+ - -)|a194>t,K65>I(- + -)| t196>c,Y66>H(+ - -)|g202>c,A68>P(- - -)|a215>g,K72>R(+ + +)|g258>c|g261>a|g267>a|c268>g,Q90>E(+ + -)|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|c280>g,L94>V(+ - +)|g286>c,A96>P(- - -)| a318>g| 320 225 202 23 6 17 5 6 2 1 0 0 1 0 4 2 1 1 78 4 3 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 36 24 21 3 0 3 1 1 1 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 30 24 19 5 1 4 1 2 0 0 0 0 1 0 0 0 0 1 117 114 103 11 3 8 1 3 1 1 0 0 0 0 3 2 0 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagtgactatggc atgcactgggtccgccaggctccaggcgaggggctggattgggtggcattt atacgatatgatggaaatgagata cactatccagactccgtgaggggccgattcaccatctccagagacaattccaagaacaccctatatctagaaatgaacaatgtgagacctgaggacacggctgtgtattactgt gcgaaggaggaatttgacactggctggacacaaccgggcgac AKEEFDTGWTQPGD IGHV3-30 IGHD6-19 IGHJ4 ggattcatcttcagtgactatggc atgcactgggtccgccaggctccaggcgaggggctggattgggtggcattt atacgatatgatggaaatgagata cactatccagactccgtgaggggccgattcaccatctccagagacaattccaagaacaccctatatctagaaatgaacaatgtgagacctgaggacacggctgtgtattactgt gcgaaggaggaatttgacactggctggacacaaccgggcgac IGA2 21 213 13 8 8 14 5 7 3 1 10 7 JY8QFUQ01AEDZX 6482 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 976 94.91 205/216 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-19*01 F 2 8 8 12 8.8.12 [1.17.38.11] CAKDQAVAGGFDYW in-frame - tctggatttacctttagctcctatgccatgacctgggtccgccaggctccagggaaggggctggagtgggtcgcaattattagtggtagtgatggtcgcacatactacgccgactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaataaacagcctgagagccgaggacacggccgtatattactgtgcgaaagatcaagcagtggctggaggatttgactactggggccagggaatcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [284] c84>t|a106>t|g107>c|g119>c,S40>T(+ - +)|t160>g,S54>A(- + -)|g163>a,A55>I(+ - +)|c164>t,A55>I(+ - +)|g185>a,G62>D(- - -)|a190>c,S64>R(- - -)|a204>c|g273>a,M91>I(+ + -)| c84>t|a106>t|g107>c| g119>c,S40>T(+ - +)|t160>g,S54>A(- + -)|g163>a,A55>I(+ - +)|c164>t,A55>I(+ - +)| g185>a,G62>D(- - -)|a190>c,S64>R(- - -)| a204>c|g273>a,M91>I(+ + -)| 320 224 213 11 4 7 0 3 2 0 2 0 1 0 2 0 0 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 3 0 0 0 1 0 0 0 1 0 1 0 0 0 51 51 47 4 0 4 0 1 1 0 0 0 0 0 1 0 0 1 30 24 22 2 0 2 0 1 0 0 1 0 0 0 0 0 0 0 117 114 112 2 1 1 0 1 0 0 1 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggatttacctttagctcctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtcgcaatt attagtggtagtgatggtcgcaca tactacgccgactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaataaacagcctgagagccgaggacacggccgtatattactgt gcgaaagatcaagcagtggctggaggatttgactac AKDQAVAGGFDY IGHV3-23 IGHD6-19 IGHJ4 ggatttacctttagctcctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtcgcaatt attagtggtagtgatggtcgcaca tactacgccgactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaataaacagcctgagagccgaggacacggccgtatattactgt gcgaaagatcaagcagtggctggaggatttgactac IGG1 11 213 5 6 5 7 0 4 1 3 5 2 JY8QFUQ01AEE83 12084 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F or Homsap IGHV3-21*03 F (see comment) 741 83.18 178/214 nt NA Homsap IGHJ6*02 F 179 77.05 47/61 nt Homsap IGHD2-15*01 F 3 8 7 12 8.7.12 [1.17.38.11] CARDKVISAGRDVW in-frame + The submitted sequence and the closest germline V-GENE allele show different CDR2-IMGT amino acid lengths (7 AA in JY8QFUQ01AEE83 ; 8 AA in Homsap_IGHV3-21*01), and low V-REGION identity (83,18% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctggattcagtttcactggttttaccgtgatctgggtccgccaggctccaaggaaggggctggaatggatctcatccgtcactactaatggtctcacgtactacgcagactcagtagagggccgattcaacatctccagggacaacgccaacaatttagtgtttctgcaaatgaacagcctgagagtcgaggacactggtgtatattattgtgcgagagataaagtgatctctgccggacgggacgtctggggccaagggaccacggtcaccgtctcctcaacatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [281] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>c,S35>T(+ - +)|a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)|a110>t,Y37>F(- + -)|g113>c,S38>T(+ - +)|a115>g,M39>V(+ - -)|a119>t,N40>I(- - -)|g139>a,G47>R(- - -)|g153>a|g157>a,V53>I(+ - +)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>c,S57>T(+ - +)|g173>c,S58>T(+ - +)|g176>a,S59>N(- - -)|a187>g,S63>G(+ + -)|t190>c,Y64>L(- - -)|a191>t,Y64>L(- - -)|t194>c,I65>T(- - -)|a195>g,I65>T(- - -)|g213>a|a214>g,K72>E(+ - -)|c230>a,T77>N(- + -)|a240>g|g252>c,K84>N(+ - -)|c255>t|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|g297>t|c299>g,A100>G(- + -)|g303>a|c309>t| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>c,S35>T(+ - +)|a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)|a110>t,Y37>F(- + -)|g113>c,S38>T(+ - +)| a115>g,M39>V(+ - -)|a119>t,N40>I(- - -)|g139>a,G47>R(- - -)|g153>a|g157>a,V53>I(+ - +)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>c,S57>T(+ - +)|g173>c,S58>T(+ - +)|g176>a,S59>N(- - -)|a187>g,S63>G(+ + -)|t190>c,Y64>L(- - -)|a191>t,Y64>L(- - -)|t194>c,I65>T(- - -)|a195>g,I65>T(- - -)| g213>a|a214>g,K72>E(+ - -)|c230>a,T77>N(- + -)|a240>g|g252>c,K84>N(+ - -)|c255>t|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|g297>t|c299>g,A100>G(- + -)|g303>a|c309>t| 320 222 186 36 7 29 7 6 6 3 0 1 4 0 5 3 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 0 7 1 0 2 0 0 0 1 0 2 1 0 0 51 51 46 5 1 4 1 3 0 0 0 0 1 0 0 0 0 0 30 21 11 10 0 10 3 1 0 3 0 0 1 0 2 0 0 0 117 114 100 14 6 8 2 2 4 0 0 1 1 0 1 2 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagtttcactggttttacc gtgatctgggtccgccaggctccaaggaaggggctggaatggatctcatcc gtcactactaatggtctcacg tactacgcagactcagtagagggccgattcaacatctccagggacaacgccaacaatttagtgtttctgcaaatgaacagcctgagagtcgaggacactggtgtatattattgt gcgagagataaagtgatctctgccggacgggacgtc ARDKVISAGRDV IGHV3-21 IGHD2-15 IGHJ6 ggattcagtttcactggttttacc gtgatctgggtccgccaggctccaaggaaggggctggaatggatctcatcc gtcactactaatggtctcacg tactacgcagactcagtagagggccgattcaacatctccagggacaacgccaacaatttagtgtttctgcaaatgaacagcctgagagtcgaggacactggtgtatattattgt gcgagagataaagtgatctctgccggacgggacgtc IGA2 36 210 22 14 12 22 10 14 7 0 12 17 JY8QFUQ01AEF8J 12403 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 995 95.41 208/218 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-13*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKDPFIAATGRPWYFDNW in-frame + cccctggattcaccttcagtagttatggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagttatatcatatgatggaagtgataaatactatgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtttctgcaaatgaacagcctgagaagtgaagacacggctgactattactgtgcgaaagacccatttatagcagcaactggccgcccctggtattttgacaactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [300] t76>c,S26>P(+ - -)|c108>t|a190>g,N64>D(+ + -)|a225>g|a263>t,Y88>F(- + -)|g286>a,A96>S(- + -)|c287>g,A96>S(- + -)|g291>a|t302>a,V101>D(- - -)|g303>c,V101>D(- - -)|g317>a,R106>K(+ + +)| t76>c,S26>P(+ - -)| c108>t| a190>g,N64>D(+ + -)| a225>g|a263>t,Y88>F(- + -)|g286>a,A96>S(- + -)|c287>g,A96>S(- + -)|g291>a|t302>a,V101>D(- - -)|g303>c,V101>D(- - -)| g317>a,R106>K(+ + +)| 320 226 215 11 3 8 2 3 1 1 0 0 1 1 1 1 0 0 78 5 4 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 117 114 107 7 2 5 1 2 0 0 0 0 1 1 1 1 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cccct ggattcaccttcagtagttatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcatatgatggaagtgataaa tactatgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtttctgcaaatgaacagcctgagaagtgaagacacggctgactattactgt gcgaaagacccatttatagcagcaactggccgcccctggtattttgacaac AKDPFIAATGRPWYFDN IGHV3-30 IGHD6-13 IGHJ4 ggattcaccttcagtagttatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcatatgatggaagtgataaa tactatgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtttctgcaaatgaacagcctgagaagtgaagacacggctgactattactgt gcgaaagacccatttatagcagcaactggccgcccctggtattttgacaac IGA1 9 213 5 4 3 5 2 4 2 1 5 1 JY8QFUQ01AEH4G 12089 productive (see comment) Homsap IGHV3-48*01 F 855 88.48 192/217 nt 87.56 190/217 nt Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD3-22*01 F 2 8 8 19 8.8.19 [1.16.38.11] CARASTFYYDGSGYRDVFDIW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 35 of V-REGION: 'G' (1 nucleotides at position 18 to 18 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 47 of V-REGION: 1 nucleotides (from position 56 in the user submitted sequence), (cause frameshift) tctctggcttcagtttgGagtacttataccatgaactgggtccgccaggctccaggaaggggctggagtgggtttcactcattagtaagactagtaatgtcatatactacgcggactctgtgaagggccggttcaccatctccagagacaatgccgagaattcactgtttctgcaaatggacagcctgagtgccgaggacacgggtgtatattactgtgcgagagcttcgacgttctactacgatggcagtggttatcgtgatgtttttgatatatggggccaagggacaatggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [307] c74>t|a81>c|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g113>c,S38>T(+ - +)|t163>c,Y55>L(- - -)|a164>t,Y55>L(- - -)|g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g176>c,S59>T(+ - +)|g188>a,S63>N(- - -)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)|a204>g|a225>g|a250>g,K84>E(+ - -)|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|a285>t,R95>S(- - -)|c299>g,A100>G(- + -)|g303>a| c74>t| a81>c|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g113>c,S38>T(+ - +)| t163>c,Y55>L(- - -)|a164>t,Y55>L(- - -)| g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g176>c,S59>T(+ - +)|g188>a,S63>N(- - -)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)| a204>g|a225>g|a250>g,K84>E(+ - -)|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|a285>t,R95>S(- - -)|c299>g,A100>G(- + -)|g303>a| a320>c| 319 (320) 224 (225) 199 25 (26) 6 (7) 19 5 3 5 1 1 (2) 0 3 0 3 3 0 1 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 17 7 1 6 0 0 2 0 1 0 0 0 2 2 0 0 51 50 48 2 0 2 0 0 0 1 0 0 1 0 0 0 0 0 30 24 18 6 0 6 1 2 1 0 0 0 0 0 1 0 0 1 117 114 105 9 4 5 4 1 1 0 0 0 2 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tctct ggcttcagtttgagtacttatacc atgaactgggtccgccaggctccaggaaggggctggagtgggtttcactc attagtaagactagtaatgtcata tactacgcggactctgtgaagggccggttcaccatctccagagacaatgccgagaattcactgtttctgcaaatggacagcctgagtgccgaggacacgggtgtatattactgt gcgagagcttcgacgttctactacgatggcagtggttatcgtgatgtttttgatata ARASTFYYDGSGYRDVFDI IGHV3-48 IGHD3-22 IGHJ3 ggcttcagtttgagtacttatacc atgaactgggtccgccaggctccaggaaggggctggagtgggtttcactc attagtaagactagtaatgtcata tactacgcggactctgtgaagggccggttcaccatctccagagacaatgccgagaattcactgtttctgcaaatggacagcctgagtgccgaggacacgggtgtatattactgt gcgagagcttcgacgttctactacgatggcagtggttatcgtgatgtttttgatata IGG1 24 212 13 11 7 13 6 11 4 1 7 12 JY8QFUQ01AEM2N 7747 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 849 88.79 190/214 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD3-16*02 F 2 8 7 19 8.7.19 [1.17.38.11] CARSSDYLWGSYRYPYYFDYW in-frame + ctctggtgactccatcagtagttacttctggagttggatccggcagcccccagggaagggactggagtggattggatatgtctattacagtggaagtaccaagtataatccttccctcgaaagtcgagtcaccatatcattagacacgcccaacaaccagttctccctgagcctgacctatgtcaccgctgcggacacggccatatactactgtgcgagaagctctgattacctttgggggagttatcgttatccctactacttcgactactggagccagggaaccctggtcaccgtctccttagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [302] g83>a,G28>D(- - -)|a113>t,Y38>F(- + -)|c120>t|g162>a|a166>g,I56>V(+ - +)|g189>a|c192>t|c198>g,N66>K(+ - -)|c201>t|c204>t|c207>t|a214>g,K72>E(+ - -)|g216>a,K72>E(+ - -)|g238>t,V80>L(+ - +)|t247>c,S83>P(+ - -)|g252>c,K84>N(+ - -)|a269>g,K90>S(- - -)|g270>c,K90>S(- - -)|g275>c,S92>T(+ - +)|c278>a,S93>Y(+ - -)|g282>c|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|t306>c| g83>a,G28>D(- - -)|a113>t,Y38>F(- + -)| c120>t|g162>a| a166>g,I56>V(+ - +)|g189>a|c192>t| c198>g,N66>K(+ - -)|c201>t|c204>t|c207>t|a214>g,K72>E(+ - -)|g216>a,K72>E(+ - -)|g238>t,V80>L(+ - +)|t247>c,S83>P(+ - -)|g252>c,K84>N(+ - -)|a269>g,K90>S(- - -)|g270>c,K90>S(- - -)|g275>c,S92>T(+ - +)|c278>a,S93>Y(+ - -)|g282>c|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|t306>c| g319>a|a320>g| 318 (320) 220 (222) 196 24 (26) 9 (11) 15 3 (4) 6 (7) 5 2 0 1 1 0 4 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 1 0 0 0 0 0 51 51 49 2 2 0 0 1 1 0 0 0 0 0 0 0 0 0 30 21 18 3 2 1 1 1 1 0 0 0 0 0 0 0 0 0 117 114 97 17 5 12 2 3 3 2 0 1 0 0 4 1 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtgactccatcagtagttacttc tggagttggatccggcagcccccagggaagggactggagtggattggatat gtctattacagtggaagtacc aagtataatccttccctcgaaagtcgagtcaccatatcattagacacgcccaacaaccagttctccctgagcctgacctatgtcaccgctgcggacacggccatatactactgt gcgagaagctctgattacctttgggggagttatcgttatccctactacttcgactac ARSSDYLWGSYRYPYYFDY IGHV4-59 IGHD3-16 IGHJ4 ggtgactccatcagtagttacttc tggagttggatccggcagcccccagggaagggactggagtggattggatat gtctattacagtggaagtacc aagtataatccttccctcgaaagtcgagtcaccatatcattagacacgcccaacaaccagttctccctgagcctgacctatgtcaccgctgcggacacggccatatactactgt gcgagaagctctgattacctttgggggagttatcgttatccctactacttcgactac IGA1 24 210 16 8 11 18 5 6 7 2 12 3 JY8QFUQ01AEMXL 5375 productive (see comment) Homsap IGHV3-23*05 F 831 87.85 188/214 nt 87.38 187/214 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD1-14*01 ORF 1 8 7 10 8.7.10 [1.17.38.11] CAKRPPGTIDRW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 63 of V-REGION: 3 nucleotides (from position 95 in the user submitted sequence), (do not cause frameshift) ctctggattcacctttggcacctctgacatggcctgggtccgccaggttccaggggaggggctggagtgggtctcacacattgatatcagaggtgccacacagtataaagactccgtgaagggccggttcaccatctccagagacaattccaagagcactctatatctgcaaatgaacaccttgcgagccgaggacacggccgtatattactgtgcgaaacgcccgcccggcaccatagatcgctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaaccgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 89 [275, 276] a103>g,S35>G(+ + -)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|c113>a,A38>D(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g163>c,A55>H(- - -)|c164>a,A55>H(- - -)|t165>c,A55>H(- - -)|t169>g,Y57>D(- - -)|g173>t,S58>I(- - -)|t177>a,S59>R(- - -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|t196>c,Y66>Q(- - -)|c198>g,Y66>Q(- - -)|g202>a,A68>K(- - -)|c203>a,A68>K(- - -)|a254>g,N85>S(- - -)|g258>t|g261>a|g278>c,S93>T(+ - +)|c280>t|a283>c| a103>g,S35>G(+ + -)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|c113>a,A38>D(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g163>c,A55>H(- - -)|c164>a,A55>H(- - -)|t165>c,A55>H(- - -)| t169>g,Y57>D(- - -)|g173>t,S58>I(- - -)|t177>a,S59>R(- - -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| t196>c,Y66>Q(- - -)|c198>g,Y66>Q(- - -)|g202>a,A68>K(- - -)|c203>a,A68>K(- - -)|a254>g,N85>S(- - -)|g258>t|g261>a|g278>c,S93>T(+ - +)|c280>t|a283>c| 318 220 194 26 4 22 5 2 2 2 2 3 0 1 5 1 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 0 0 0 1 1 0 0 1 0 0 0 51 51 44 7 0 7 2 0 1 1 0 1 0 0 2 0 0 0 30 21 16 5 0 5 1 0 0 0 0 0 0 1 1 0 1 1 117 114 104 10 4 6 1 2 1 1 1 1 0 0 1 1 1 0 6 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|agca,172-175(CDR2)|ggta,184-187(CDR2)|agca,190-193(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttggcacctctgac atggcctgggtccgccaggttccaggggaggggctggagtgggtctcacac attgatatcagaggtgccaca cagtataaagactccgtgaagggccggttcaccatctccagagacaattccaagagcactctatatctgcaaatgaacaccttgcgagccgaggacacggccgtatattactgt gcgaaacgcccgcccggcaccatagatcgc AKRPPGTIDR IGHV3-23 IGHD1-14 IGHJ4 ggattcacctttggcacctctgac atggcctgggtccgccaggttccaggggaggggctggagtgggtctcacac attgatatcagaggtgccaca cagtataaagactccgtgaagggccggttcaccatctccagagacaattccaagagcactctatatctgcaaatgaacaccttgcgagccgaggacacggccgtatattactgt gcgaaacgcccgcccggcaccatagatcgc IGA2 26 210 11 15 4 15 7 11 4 0 13 9 JY8QFUQ01AENJP 18932 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 959 93.58 204/218 nt NA Homsap IGHJ2*01 F 247 96.23 51/53 nt Homsap IGHD2-21*01 F 1 8 8 12 8.8.12 [1.17.38.11] CAKVVWSLWYFDLW in-frame + cctctggattcaccttcaacaactatgccatgagttgggtccgccaggctccagggaaggggctggaatgggtctcaactattactagtggtggtggtagtacattgtacgcagactccgtgaagggccggttcaccatctccagagacaatttcaaggacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaaagtagtctggagcctctggtacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [286] t90>c|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|c120>t|g153>a|g163>a,A55>T(- - -)|g170>c,S57>T(+ - +)|g172>a,G58>S(+ + -)|a175>g,S59>G(+ + -)|c192>t|a197>t,Y66>L(- - -)|c198>g,Y66>L(- - -)|c248>t,S83>F(- - -)|a253>g,N85>D(+ + -)| t90>c|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)| c120>t|g153>a|g163>a,A55>T(- - -)| g170>c,S57>T(+ - +)|g172>a,G58>S(+ + -)|a175>g,S59>G(+ + -)|c192>t| a197>t,Y66>L(- - -)|c198>g,Y66>L(- - -)|c248>t,S83>F(- - -)|a253>g,N85>D(+ + -)| a320>t| 319 (320) 225 (226) 211 14 (15) 4 (5) 10 2 5 3 1 0 0 1 (2) 0 1 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 2 0 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 2 1 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 1 1 1 0 0 0 0 0 1 0 0 0 117 114 110 4 0 4 1 0 1 0 0 0 1 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcaacaactatgcc atgagttgggtccgccaggctccagggaaggggctggaatgggtctcaact attactagtggtggtggtagtaca ttgtacgcagactccgtgaagggccggttcaccatctccagagacaatttcaaggacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaagtagtctggagcctctggtacttcgatctc AKVVWSLWYFDL IGHV3-23 IGHD2-21 IGHJ2 ggattcaccttcaacaactatgcc atgagttgggtccgccaggctccagggaaggggctggaatgggtctcaact attactagtggtggtggtagtaca ttgtacgcagactccgtgaagggccggttcaccatctccagagacaatttcaaggacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaagtagtctggagcctctggtacttcgatctc IGG1 14 213 11 3 8 10 3 4 2 2 5 5 JY8QFUQ01AEQU4 3280 productive (see comment) Homsap IGHV4-39*03 F 902 90 198/220 nt 89.55 197/220 nt Homsap IGHJ3*01 F 232 96 48/50 nt Homsap IGHD3-22*01 F 3 10 7 17 10.7.17 [1.17.38.11] CARSITLTVVVMNDAFDVW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 50 of V-REGION: 'TT' (2 nucleotides at position 71 to 72 in the user submitted sequence), (cause frameshift); tctccggtggctccatcaacagtagaaattattattggggctggatccgccagccccccagggaagggttTTggagtggattggaaatatctattatagtgggaacacctactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgtgcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtctggggccaagggacattggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 100 100 [306] t78>c|g92>a,S31>N(- - -)|t105>a,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t|c114>t|a138>c|g139>a,G47>R(- - -)|a142>g,K48>E(+ - -)|g144>a,K48>E(+ - -)|c148>t|g162>a|g164>a,S55>N(- - -)|g191>a,S64>N(- - -)|c204>t|a232>g,I78>V(+ - +)|c245>g,T82>R(- - -)|c249>t|c261>g,F87>L(+ - -)|g275>c,S92>T(+ - +)|g280>c,V94>L(+ - +)|g303>a| t78>c| g92>a,S31>N(- - -)|t105>a,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t|c114>t| a138>c|g139>a,G47>R(- - -)|a142>g,K48>E(+ - -)|g144>a,K48>E(+ - -)|c148>t|g162>a|g164>a,S55>N(- - -)| g191>a,S64>N(- - -)| c204>t|a232>g,I78>V(+ - +)|c245>g,T82>R(- - -)|c249>t|c261>g,F87>L(+ - -)|g275>c,S92>T(+ - +)|g280>c,V94>L(+ - +)|g303>a| 311 220 198 22 9 13 2 8 5 1 1 0 0 1 2 2 0 0 78 5 4 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 30 25 5 2 3 0 2 2 0 0 0 0 1 0 0 0 0 51 51 44 7 3 4 1 4 1 0 1 0 0 0 0 0 0 0 30 21 20 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 116 114 105 8 3 5 1 1 2 0 0 0 0 0 2 2 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctcc ggtggctccatcaacagtagaaattattat tggggctggatccgccagccccccagggaagggttggagtggattggaaat atctattatagtgggaacacc tactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgt gcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtc ARSITLTVVVMNDAFDV IGHV4-39 IGHD3-22 IGHJ3 ggtggctccatcaacagtagaaattattat tggggctggatccgccagccccccagggaagggttggagtggattggaaat atctattatagtgggaacacc tactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgt gcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtc IGG1 21 216 15 6 13 17 2 4 6 2 9 4 JY8QFUQ01AEU6X 15503 productive Homsap IGHV3-11*01 F 865 88.58 194/219 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 201 88.24 45/51 nt Homsap IGHD4-17*01 F 2 8 8 17 8.8.17 [2.17.38.11] CARDGDADYGDLPIPFDSW in-frame - Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) gcctctgaattcagtttcactgaccaccacatgagctggatccgccgggctccagggaaggggctggagtgggtgtcatacattagtcctacaggtagtgccatattttacgcagactctgtgaaggcccgtttcaccatctctagggacaacgccaagaatttactatatctacaaatgaacagcctgagacccgaggacacggccatctattactgtgcgagagatggggacgcggactacggtgacttgcccatccctttcgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [301] g80>a,G27>E(- - -)|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>c,S35>T(+ - +)|t109>c,Y37>H(+ - -)|t112>c,Y38>H(+ - -)|a131>g,Q44>R(+ - -)|t159>g|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g176>c,S59>T(+ - +)|t177>a,S59>T(+ - +)|a190>g,T64>A(- - -)|a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|g221>c,G74>A(- + -)|a225>t|c237>t|c255>t|c257>t,S86>L(- - -)|g261>a|g267>a|g286>c,A96>P(- - -)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)| g80>a,G27>E(- - -)|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>c,S35>T(+ - +)|t109>c,Y37>H(+ - -)|t112>c,Y38>H(+ - -)| a131>g,Q44>R(+ - -)|t159>g| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g176>c,S59>T(+ - +)|t177>a,S59>T(+ - +)|a190>g,T64>A(- - -)| a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|g221>c,G74>A(- + -)|a225>t|c237>t|c255>t|c257>t,S86>L(- - -)|g261>a|g267>a|g286>c,A96>P(- - -)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)| 320 227 202 25 6 19 2 4 5 2 1 0 2 1 6 1 0 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 1 1 2 0 0 0 0 1 1 0 0 51 51 49 2 1 1 1 0 0 0 0 0 0 0 0 0 0 1 30 24 19 5 0 5 1 0 0 0 1 0 0 1 2 0 0 0 117 114 102 12 5 7 0 3 4 0 0 0 2 0 3 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct gaattcagtttcactgaccaccac atgagctggatccgccgggctccagggaaggggctggagtgggtgtcatac attagtcctacaggtagtgccata ttttacgcagactctgtgaaggcccgtttcaccatctctagggacaacgccaagaatttactatatctacaaatgaacagcctgagacccgaggacacggccatctattactgt gcgagagatggggacgcggactacggtgacttgcccatccctttcgactcc ARDGDADYGDLPIPFDS IGHV3-11 IGHD4-17 IGHJ5 gaattcagtttcactgaccaccac atgagctggatccgccgggctccagggaaggggctggagtgggtgtcatac attagtcctacaggtagtgccata ttttacgcagactctgtgaaggcccgtttcaccatctctagggacaacgccaagaatttactatatctacaaatgaacagcctgagacccgaggacacggccatctattactgt gcgagagatggggacgcggactacggtgacttgcccatccctttcgactcc IGA2 25 213 13 12 9 16 4 9 6 0 8 11 JY8QFUQ01AEVWH 13455 productive Homsap IGHV3-7*01 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-10*01 F 2 8 8 18 8.8.18 [1.17.38.11] CAKARIYFYGSGSYSLFDYW in-frame + ctctggaatcaccttgagtccctattggatgacctgggtccgccaggctcccgggaaggggctggagtgggtggccaacataaaccaagatggaggtgagagaaattatgtggcctctgtgaggggccggttcaccatctccagagacaacgccaggaattcactgtatctgcaaatgaacagcctgagagtcgacgacacggctgtatattattgtgcaaaagccaggatctatttttatggttcggggagttattccctctttgactactggggccagggagccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 100 100 [303] t82>a,F28>I(+ - -)|t90>g,F30>L(+ - -)|a106>c,S36>P(+ - -)|g107>c,S36>P(+ - -)|g119>c,S40>T(+ - +)|a138>c|g171>c,K57>N(+ - -)|a187>g,S63>G(+ + -)|a194>g,K65>R(+ + +)|t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a225>g|a251>g,K84>R(+ + +)|c255>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>a|c309>t|g315>a|g317>a,R106>K(+ + +)| t82>a,F28>I(+ - -)|t90>g,F30>L(+ - -)|a106>c,S36>P(+ - -)|g107>c,S36>P(+ - -)| g119>c,S40>T(+ - +)|a138>c| g171>c,K57>N(+ - -)|a187>g,S63>G(+ + -)|a194>g,K65>R(+ + +)| t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a225>g|a251>g,K84>R(+ + +)|c255>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>a|c309>t| g315>a|g317>a,R106>K(+ + +)|a320>c| 319 (320) 224 (225) 202 22 (23) 6 (7) 16 5 3 4 0 3 (4) 0 0 2 4 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 0 1 0 0 1 1 0 0 1 51 51 49 2 1 1 0 0 0 0 1 0 0 0 1 0 0 0 30 24 21 3 0 3 2 0 0 0 0 0 0 0 1 0 0 0 117 114 103 11 4 7 3 1 4 0 1 0 0 1 1 0 0 0 7 (8) 7 5 2 (3) 1 (2) 1 0 2 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggaatcaccttgagtccctattgg atgacctgggtccgccaggctcccgggaaggggctggagtgggtggccaac ataaaccaagatggaggtgagaga aattatgtggcctctgtgaggggccggttcaccatctccagagacaacgccaggaattcactgtatctgcaaatgaacagcctgagagtcgacgacacggctgtatattattgt gcaaaagccaggatctatttttatggttcggggagttattccctctttgactac AKARIYFYGSGSYSLFDY IGHV3-7 IGHD3-10 IGHJ4 ggaatcaccttgagtccctattgg atgacctgggtccgccaggctcccgggaaggggctggagtgggtggccaac ataaaccaagatggaggtgagaga aattatgtggcctctgtgaggggccggttcaccatctccagagacaacgccaggaattcactgtatctgcaaatgaacagcctgagagtcgacgacacggctgtatattattgt gcaaaagccaggatctatttttatggttcggggagttattccctctttgactac IGG1 20 213 10 10 5 9 5 11 5 0 8 7 JY8QFUQ01AEX25 4398 productive (see comment) Homsap IGHV3-11*01 F 999 95.85 208/217 nt 95.39 207/217 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD5-18*01 F 3 8 8 11 8.8.11 [1.17.38.11] CARGYSYGCFDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 59 of V-REGION: 'GGAGGGACT' (9 nucleotides at position 89 to 97 in the user submitted sequence), (do not cause frameshift); ctctggattcaccttcagtgactactacatgaactggatccgccaggctccagggaaggggctggagtggatttcaaacatcaatagtGGAGGGACTagtgggaggaccatatattacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgagaggatacagctatggctgctttgactactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 100 100 [290] g119>a,S40>N(- - -)|g157>a,V53>I(+ - +)|t163>a,Y55>N(- - -)|t168>c|g170>a,S57>N(- - -)|t186>g|t189>g,S63>R(- - -)|c198>t|g303>a| g119>a,S40>N(- - -)|g157>a,V53>I(+ - +)|t163>a,Y55>N(- - -)| t168>c|g170>a,S57>N(- - -)|t186>g|t189>g,S63>R(- - -)| c198>t|g303>a| a320>g| 319 (320) 224 (225) 215 9 (10) 4 (5) 5 0 (1) 4 1 1 0 0 0 1 0 0 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 48 3 0 3 0 2 0 0 0 0 0 1 0 0 0 0 30 24 20 4 2 2 0 1 0 1 0 0 0 0 0 0 0 2 117 114 112 2 2 0 0 1 1 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgactactac atgaactggatccgccaggctccagggaaggggctggagtggatttcaaac atcaatagtagtgggaggaccata tattacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagaggatacagctatggctgctttgactac ARGYSYGCFDY IGHV3-11 IGHD5-18 IGHJ4 ggattcaccttcagtgactactac atgaactggatccgccaggctccagggaaggggctggagtggatttcaaac atcaatagtagtgggaggaccata tattacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagaggatacagctatggctgctttgactac IGA2 9 213 6 3 5 5 1 4 2 2 3 2 JY8QFUQ01AEYEY 3488 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 1014 95.91 211/220 nt 95.45 210/220 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 8 8 8.8.8 [2.17.38.11] CARDNYGDYW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 62 of V-REGION: 'C' (1 nucleotides at position 95 to 95 in the user submitted sequence), (cause frameshift); agcctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtgttaatggtgatCgggggtagcacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [276] a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| 320 228 219 9 4 5 4 1 0 0 0 0 0 0 3 0 1 0 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 3 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 4 2 1 1 0 0 0 0 0 0 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac IGA1 9 213 5 4 1 5 4 4 4 0 2 3 JY8QFUQ01AEYN1 15972 productive (see comment) Homsap IGHV3-7*01 F 995 95.41 208/218 nt 94.95 207/218 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-15*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAKGGGNGDLDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'G' (1 nucleotides at position 9 to 9 in the user submitted sequence), (cause frameshift); cctctggaGttcacctttagtaaccattggatgaactgggtccgccaggctccagggaaggggctggagtgggtggccaacataatgccagatggaggtgagaaattctatgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgaaggggggtggtaacggcgaccttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [283] g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)|g119>a,S40>N(- - -)|a170>t,K57>M(- + -)|a173>c,Q58>P(- - -)|a187>g,S63>G(+ + -)|a197>t,Y66>F(- + -)|a225>g|a245>g,N82>S(- - -)|c265>t| g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)| g119>a,S40>N(- - -)| a170>t,K57>M(- + -)|a173>c,Q58>P(- - -)|a187>g,S63>G(+ + -)| a197>t,Y66>F(- + -)|a225>g|a245>g,N82>S(- - -)|c265>t| g317>a,R106>K(+ + +)|a318>g,R106>K(+ + +)|a320>g| 316 (320) 222 (226) 212 (213) 10 (13) 2 (3) 8 (10) 3 (5) 2 (3) 1 1 1 0 2 0 0 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 0 0 1 0 1 0 0 0 0 0 117 114 110 4 2 2 2 0 1 0 0 0 1 0 0 0 0 0 4 (8) 4 4 (5) 0 (3) 0 (1) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagtaaccattgg atgaactgggtccgccaggctccagggaaggggctggagtgggtggccaac ataatgccagatggaggtgagaaa ttctatgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgaaggggggtggtaacggcgaccttgactac AKGGGNGDLDY IGHV3-7 IGHD2-15 IGHJ4 ggattcacctttagtaaccattgg atgaactgggtccgccaggctccagggaaggggctggagtgggtggccaac ataatgccagatggaggtgagaaa ttctatgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgaaggggggtggtaacggcgaccttgactac IGA2 10 213 7 3 3 3 4 7 2 0 3 5 JY8QFUQ01AEYTS 11633 productive Homsap IGHV3-7*02 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD5-12*01 F 1 8 8 9 8.8.9 [1.17.38.11] CARDQVTEDYW in-frame + ctctggattcctttttagaacctattggatgagttgggtctgccaggctccagggaaagggctggagtgggtggcccacataaaccaagatggacgtgaggcatactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgtgccagagatcaagtgactgaagactactggggccagggaaccctggtcagtgtctcctcagcttccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG3 100 100 [276] a85>c,T29>L(- - -)|c86>t,T29>L(- - -)|c87>t,T29>L(- - -)|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|c120>t|c127>t,R43>C(- - -)|a163>c,N55>H(- - -)|g171>c,K57>N(+ - -)|a187>c,S63>R(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)|c248>g,A83>G(- + -)|c255>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|g297>c|g301>a,V101>M(+ - -)|c309>t|g315>c| a85>c,T29>L(- - -)|c86>t,T29>L(- - -)|c87>t,T29>L(- - -)|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)| c120>t|c127>t,R43>C(- - -)|a163>c,N55>H(- - -)| g171>c,K57>N(+ - -)|a187>c,S63>R(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)| c248>g,A83>G(- + -)|c255>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|g297>c|g301>a,V101>M(+ - -)|c309>t| g315>c| 318 223 202 21 6 15 1 2 7 0 4 0 0 1 4 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 0 2 0 1 0 0 1 1 0 0 0 51 51 48 3 1 2 0 0 2 0 1 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 0 0 2 0 0 0 1 0 0 0 117 114 106 8 4 4 0 2 3 0 0 0 0 0 1 2 0 0 6 6 5 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcctttttagaacctattgg atgagttgggtctgccaggctccagggaaagggctggagtgggtggcccac ataaaccaagatggacgtgaggca tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac ARDQVTEDY IGHV3-7 IGHD5-12 IGHJ4 ggattcctttttagaacctattgg atgagttgggtctgccaggctccagggaaagggctggagtgggtggcccac ataaaccaagatggacgtgaggca tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac IGG3 20 213 10 10 9 14 1 6 5 0 6 9 JY8QFUQ01AF30Y 21886 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 872 89.77 193/215 nt 87.91 189/215 nt Homsap IGHJ3*02 F 214 92 46/50 nt Homsap IGHD2-21*01 F 2 8 7 15 8.7.15 [1.17.38.11] CTRELRLAAARDGVDIW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'G' (1 nucleotides at position 49 to 49 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 53 of V-REGION: 'G' (1 nucleotides at position 73 to 73 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 55 of V-REGION: 'G' (1 nucleotides at position 81 to 81 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 59 of V-REGION: 'C' (1 nucleotides at position 94 to 94 in the user submitted sequence), (cause frameshift); ctctggggttcaccgtcagtagcaagttcatgagctgggtccgccaggGgtccagggaaggggctggagtggGgtctcagGttacttatcctgCatggtactacacattatagagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgtacgagagaacttaggctggctgctgcgcgtgatggtgttgacatttggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcgtcgcatgc NA IGA2 86 95 [295, 296] c75>t|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)|c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)|c134>g,A45>G(- + -)|t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|t262>g,Y88>D(- - -)|g303>t|c309>t|g313>a,A105>T(- - -)| c75>t|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)| c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)| c134>g,A45>G(- + -)| t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)| t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|t262>g,Y88>D(- - -)|g303>t|c309>t| g313>a,A105>T(- - -)| 320 223 200 23 4 19 0 3 7 3 1 0 1 0 2 3 1 2 78 5 1 4 1 3 0 0 2 1 0 0 0 0 0 0 0 1 36 24 22 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 21 14 7 0 7 0 1 2 1 1 0 0 0 2 0 0 0 117 114 106 8 3 5 0 1 3 1 0 0 0 0 0 1 1 1 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctctg gggttcaccgtcagtagcaagttc atgagctgggtccgccagggtccagggaaggggctggagtgggtctcagtt acttatcctgatggtactaca cattatagagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttaggctggctgctgcgcgtgatggtgttgacatt TRELRLAAARDGVDI IGHV3-53 IGHD2-21 IGHJ3 gggttcaccgtcagtagcaagttc atgagctgggtccgccagggtccagggaaggggctggagtgggtctcagtt acttatcctgatggtactaca cattatagagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttaggctggctgctgcgcgtgatggtgttgacatt IGA2 18 210 9 9 7 13 2 5 3 0 6 9 JY8QFUQ01AF3QZ 12348 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 927 92.17 200/217 nt NA Homsap IGHJ6*02 F (see comment) 184 77.42 48/62 nt Homsap IGHD6-25*01 F 2 8 8 14 8.8.14 [1.17.38.11] CAREVIPTATYGMDLW in-frame + Other possibilities: Homsap_IGHJ4*01 (shorter alignment but highest percentage of identity) gtctggattcacgtttagtagatttgtcatccattgggtccgccagactccaggcaaggcgctggagtgggtggcagttatttggtatgatggaactaacaaatactatacagaatccgtgaagggccgattcaccatctccagagacaattccaagaacatgctgtatctgcaaatgaacagcctgagagccgaggacacggctgtttactactgtgcgagagaggtcataccaactgccacctacggcatggacctctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [291] c87>g|c90>t|c108>a,S36>R(- - -)|a110>t,Y37>F(- + -)|g113>t,G38>V(- - -)|g117>c,M39>I(+ + -)|c120>t|g133>a,A45>T(- - -)|g146>c,G49>A(- + -)|a168>t|g188>c,S63>T(+ - +)|t192>c|g202>a,A68>T(- - -)|c207>a,D69>E(+ - +)|c257>t,T86>M(- - -)|g303>t|t306>c| c87>g|c90>t|c108>a,S36>R(- - -)|a110>t,Y37>F(- + -)|g113>t,G38>V(- - -)| g117>c,M39>I(+ + -)|c120>t|g133>a,A45>T(- - -)|g146>c,G49>A(- + -)| a168>t|g188>c,S63>T(+ - +)|t192>c| g202>a,A68>T(- - -)|c207>a,D69>E(+ - +)|c257>t,T86>M(- - -)|g303>t|t306>c| 320 225 208 17 7 10 0 2 3 2 0 2 2 0 3 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 0 1 0 0 1 1 0 0 1 1 0 51 51 47 4 1 3 0 1 1 0 0 0 0 0 2 0 0 0 30 24 21 3 2 1 0 0 0 1 0 0 1 0 1 0 0 0 117 114 109 5 2 3 0 1 1 1 0 1 0 0 0 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcacgtttagtagatttgtc atccattgggtccgccagactccaggcaaggcgctggagtgggtggcagtt atttggtatgatggaactaacaaa tactatacagaatccgtgaagggccgattcaccatctccagagacaattccaagaacatgctgtatctgcaaatgaacagcctgagagccgaggacacggctgtttactactgt gcgagagaggtcataccaactgccacctacggcatggacctc AREVIPTATYGMDL IGHV3-33 IGHD6-25 IGHJ6 ggattcacgtttagtagatttgtc atccattgggtccgccagactccaggcaaggcgctggagtgggtggcagtt atttggtatgatggaactaacaaa tactatacagaatccgtgaagggccgattcaccatctccagagacaattccaagaacatgctgtatctgcaaatgaacagcctgagagccgaggacacggctgtttactactgt gcgagagaggtcataccaactgccacctacggcatggacctc IGG1 17 213 7 10 5 13 2 4 3 4 6 4 JY8QFUQ01AF3TS 18859 productive Homsap IGHV3-7*01 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-2*01 F 2 8 8 10 8.8.10 [1.17.38.11] CTRSSNSAYDYW in-frame - ctctggattcacgtttagtaacagttggatgggctgggcccgccaggctccagggaaggggctggagtgggtggccagcacaaaccaagatgcaagtgagaaaaagtatgtggactctgtgaggggccgattcaccatctcaagagacaacgccaagaactcactgtatttacaaatgaacagcctaagagccgaggacacggctttatatttctgtacgaggtccagtaacagcgcttatgactattggggccagggaactctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [278, 277] c87>g|g107>a,S36>N(- - -)|t109>a,Y37>S(+ - -)|a110>g,Y37>S(+ - -)|a118>g,S40>G(+ + -)|t125>c,V42>A(+ - +)|a164>g,N55>S(- - -)|t167>c,I56>T(- - -)|g171>c,K57>N(+ - -)|g185>c,G62>A(- + -)|t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|a215>g,K72>R(+ + +)|c237>a|c265>t|g267>a|g282>a|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|a308>t,Y103>F(- + -)|g313>a,A105>T(- - -)| c87>g|g107>a,S36>N(- - -)|t109>a,Y37>S(+ - -)|a110>g,Y37>S(+ - -)| a118>g,S40>G(+ + -)|t125>c,V42>A(+ - +)|a164>g,N55>S(- - -)| t167>c,I56>T(- - -)|g171>c,K57>N(+ - -)|g185>c,G62>A(- + -)| t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|a215>g,K72>R(+ + +)|c237>a|c265>t|g267>a|g282>a|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|a308>t,Y103>F(- + -)| g313>a,A105>T(- - -)|a318>g|g319>t|a320>c| 317 (320) 222 (225) 201 21 (24) 5 (8) 16 4 (5) 5 1 2 0 (1) 1 1 2 2 2 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 0 0 0 0 0 1 0 1 0 0 51 51 48 3 0 3 2 0 0 1 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 0 0 1 0 0 0 0 2 0 0 0 117 114 104 10 4 6 1 3 1 0 0 1 1 1 0 1 1 0 5 (8) 5 4 1 (4) 0 (3) 1 0 (1) 1 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgtttagtaacagttgg atgggctgggcccgccaggctccagggaaggggctggagtgggtggccagc acaaaccaagatgcaagtgagaaa aagtatgtggactctgtgaggggccgattcaccatctcaagagacaacgccaagaactcactgtatttacaaatgaacagcctaagagccgaggacacggctttatatttctgt acgaggtccagtaacagcgcttatgactat TRSSNSAYDY IGHV3-7 IGHD2-2 IGHJ4 ggattcacgtttagtaacagttgg atgggctgggcccgccaggctccagggaaggggctggagtgggtggccagc acaaaccaagatgcaagtgagaaa aagtatgtggactctgtgaggggccgattcaccatctcaagagacaacgccaagaactcactgtatttacaaatgaacagcctaagagccgaggacacggctttatatttctgt acgaggtccagtaacagcgcttatgactat IGA2 20 213 11 9 5 11 6 9 4 1 9 6 JY8QFUQ01AF4Q3 20686 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 815 86.24 188/218 nt 85.78 187/218 nt Homsap IGHJ4*02 F 105 68.75 33/48 nt Homsap IGHD2-15*01 F 2 8 8 19 8.8.19 [1.17.38.11] CAKDYGDCRGGRCYAEPLDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 61 to 61 in the user submitted sequence), (cause frameshift); catctggattcagcctcgccacttatagtatgagttgggtccgccaggctccaggaaaggGggctggagtgggtctcaggtattagtgatcatggtattgacatatactatgcagactccgtgaggggccggtttaccatctccagagacatttccaagaacacggtgtatctacaaatgaacagcctgggagtcgaggacacggccgtatattactgtgcgaaagattacggggactgtcgtggtggtagatgctacgccgagcccctcgactactggggccagggaaccctggtcacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcancgcctgcctggtccagggcttcttccc NA IGA1 79 89 [306] c75>a|c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|t90>c,F30>L(+ - -)|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>a,A38>S(- + -)|c113>g,A38>S(- + -)|c114>t,A38>S(- + -)|c120>t|g141>a|c164>g,A55>G(- + -)|g173>a,G58>D(- - -)|a175>c,S59>H(+ - -)|g176>a,S59>H(+ - -)|g187>a,G63>I(- - -)|g188>t,G63>I(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>t,T65>I(- - -)|c201>t|a215>g,K72>R(+ + +)|c228>t|a245>t,N82>I(- - -)|c259>g,L87>V(+ - +)|g267>a|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)| c75>a| c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|t90>c,F30>L(+ - -)|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>a,A38>S(- + -)|c113>g,A38>S(- + -)|c114>t,A38>S(- + -)| c120>t|g141>a|c164>g,A55>G(- + -)| g173>a,G58>D(- - -)|a175>c,S59>H(+ - -)|g176>a,S59>H(+ - -)|g187>a,G63>I(- - -)|g188>t,G63>I(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>t,T65>I(- - -)| c201>t|a215>g,K72>R(+ + +)|c228>t|a245>t,N82>I(- - -)|c259>g,L87>V(+ - +)|g267>a|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)| 320 226 196 30 6 24 4 7 7 2 1 1 1 0 2 4 1 0 78 5 4 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 36 24 14 10 0 10 1 1 2 2 0 0 0 0 2 2 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 0 0 1 0 0 30 24 16 8 0 8 1 4 1 0 1 0 0 0 0 0 1 0 117 114 106 8 3 5 2 1 3 0 0 0 1 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| catct ggattcagcctcgccacttatagt atgagttgggtccgccaggctccaggaaaggggctggagtgggtctcaggt attagtgatcatggtattgacata tactatgcagactccgtgaggggccggtttaccatctccagagacatttccaagaacacggtgtatctacaaatgaacagcctgggagtcgaggacacggccgtatattactgt gcgaaagattacggggactgtcgtggtggtagatgctacgccgagcccctcgactac AKDYGDCRGGRCYAEPLDY IGHV3-23 IGHD2-15 IGHJ4 ggattcagcctcgccacttatagt atgagttgggtccgccaggctccaggaaaggggctggagtgggtctcaggt attagtgatcatggtattgacata tactatgcagactccgtgaggggccggtttaccatctccagagacatttccaagaacacggtgtatctacaaatgaacagcctgggagtcgaggacacggccgtatattactgt gcgaaagattacggggactgtcgtggtggtagatgctacgccgagcccctcgactac IGA1 29 213 20 9 14 21 6 8 5 0 6 18 JY8QFUQ01AF4Z9 9042 productive (see comment) Homsap IGHV3-23*03 F, or Homsap IGHV3-23*05 F (see comment) 678 79.91 171/214 nt 79.44 170/214 nt Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD3-16*01 F 3 8 7 17 8.7.17 [1.17.38.11] CVRDAETTVSGVAGWFDPW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (79,91% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR2-IMGT, from codon 64 of V-REGION: 3 nucleotides (from position 98 in the user submitted sequence), (do not cause frameshift) ctctggattcagctttagcgattttgccatgagttgggtccgccaacctccaggaaaggggctggagtgggtcgcaagtgttgacagaggtggcactacatactatgcaggctccatgaagggccggctcgccgtctctagagacgatgtcgacaagacagtgagtctgcagatgaacaatctgacagtcgaggacacggccacatatcactgtgtgagagacgccgagacgacggtgtcgggagtcgctggctggttcgacccctggggccagggaaccctggtcaccgtctccgcagcttccaccaagggcccatccgtcttcccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG4 100 88 [298, 297] c86>g,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|a110>t,Y37>F(- + -)|c120>t|g132>a|g133>c,A45>P(- - -)|g141>a|t160>g,S54>A(- + -)|g163>a,V55>S(- - -)|t164>g,V55>S(- - -)|a166>g,I56>V(+ - +)|t169>g,Y57>D(- - -)|t171>c,Y57>D(- - -)|c174>a,S58>R(- - -)|t186>c|g188>c,S63>T(+ - +)|a206>g,D69>G(- - -)|g211>a,V71>M(+ - -)|t226>c,F76>L(+ - -)|a229>g,T77>A(- - -)|a232>g,I78>V(+ - +)|c237>t|t243>c|a244>g,N82>D(+ + -)|t247>g,S83>V(- - -)|c248>t,S83>V(- - -)|a250>g,K84>D(+ - -)|g252>c,K84>D(+ - -)|c255>g,N85>K(+ - -)|g258>a|c259>g,L87>V(+ - +)|t262>a,Y88>S(+ - -)|a263>g,Y88>S(+ - -)|a270>g|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|t307>c,Y103>H(+ - -)|c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)| c86>g,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|a110>t,Y37>F(- + -)| c120>t|g132>a|g133>c,A45>P(- - -)|g141>a|t160>g,S54>A(- + -)|g163>a,V55>S(- - -)|t164>g,V55>S(- - -)| a166>g,I56>V(+ - +)|t169>g,Y57>D(- - -)|t171>c,Y57>D(- - -)|c174>a,S58>R(- - -)|t186>c|g188>c,S63>T(+ - +)| a206>g,D69>G(- - -)|g211>a,V71>M(+ - -)|t226>c,F76>L(+ - -)|a229>g,T77>A(- - -)|a232>g,I78>V(+ - +)|c237>t|t243>c|a244>g,N82>D(+ + -)|t247>g,S83>V(- - -)|c248>t,S83>V(- - -)|a250>g,K84>D(+ - -)|g252>c,K84>D(+ - -)|c255>g,N85>K(+ - -)|g258>a|c259>g,L87>V(+ - +)|t262>a,Y88>S(+ - -)|a263>g,Y88>S(+ - -)|a270>g|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|t307>c,Y103>H(+ - -)| c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)| 320 222 177 45 8 37 10 8 7 6 0 1 1 1 4 3 0 4 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 1 1 0 0 0 1 0 0 1 0 0 51 51 44 7 3 4 0 3 1 0 0 0 0 0 1 0 0 2 30 21 15 6 1 5 1 0 0 2 0 1 0 0 1 0 0 1 117 114 89 25 4 21 7 4 4 4 0 0 0 1 2 2 0 1 8 8 6 2 0 2 1 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|ta,243-244(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,243-244(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,184-187(CDR2)|agca,190-193(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagctttagcgattttgcc atgagttgggtccgccaacctccaggaaaggggctggagtgggtcgcaagt gttgacagaggtggcactaca tactatgcaggctccatgaagggccggctcgccgtctctagagacgatgtcgacaagacagtgagtctgcagatgaacaatctgacagtcgaggacacggccacatatcactgt gtgagagacgccgagacgacggtgtcgggagtcgctggctggttcgacccc VRDAETTVSGVAGWFDP IGHV3-23 IGHD3-16 IGHJ5 ggattcagctttagcgattttgcc atgagttgggtccgccaacctccaggaaaggggctggagtgggtcgcaagt gttgacagaggtggcactaca tactatgcaggctccatgaagggccggctcgccgtctctagagacgatgtcgacaagacagtgagtctgcagatgaacaatctgacagtcgaggacacggccacatatcactgt gtgagagacgccgagacgacggtgtcgggagtcgctggctggttcgacccc IGG4 43 210 29 14 14 22 15 21 7 1 25 10 JY8QFUQ01AF664 20098 productive Homsap IGHV4-4*07 F 863 89.3 192/215 nt NA Homsap IGHJ3*02 F (see comment) 205 90 45/50 nt Homsap IGHD3-22*01 F 2 8 7 19 8.7.19 [1.17.38.11] CARGKDYYDTTGSLEDGFDIW in-frame + Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) tctctggtggctccataagaagttactattggagttggatacggcactccgccgggaagggactggagtggatcgggcgcatatatgacagtggtagtacaaactacaatccctccctcaagagtcgagtcagcatgtcagtggacacgtccaagaaccaggtctccctgaagttgatctctgtgaccgccgcggacacggccatgtattattgtgcgagagggaaggattactatgatacaactggctccctcgaggatggttttgatatttggggccaagggacgatggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [303] c90>a|t105>a,S35>R(- - -)|c114>t|c120>t|c126>a|g132>c,Q44>H(- + -)|c133>t,P45>S(+ - -)|t159>c|t165>c|c168>a|a172>g,T58>D(- + -)|c173>a,T58>D(- + -)|g189>t|c192>t|c195>a|c204>t|c230>g,T77>S(+ - +)|a240>g|t259>g,F87>V(+ - -)|c271>t|g275>t,S92>I(- - -)|g301>a,V101>M(+ - -)|c309>t| c90>a|t105>a,S35>R(- - -)|c114>t| c120>t|c126>a|g132>c,Q44>H(- + -)|c133>t,P45>S(+ - -)|t159>c|t165>c| c168>a|a172>g,T58>D(- + -)|c173>a,T58>D(- + -)|g189>t|c192>t|c195>a| c204>t|c230>g,T77>S(+ - +)|a240>g|t259>g,F87>V(+ - -)|c271>t|g275>t,S92>I(- - -)|g301>a,V101>M(+ - -)|c309>t| a320>g| 319 (320) 222 (223) 199 23 (24) 14 (15) 9 2 (3) 1 7 2 0 5 0 1 1 1 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 1 0 0 1 0 1 0 0 0 0 51 51 45 6 4 2 0 0 2 2 0 1 0 0 1 0 0 0 30 21 15 6 4 2 1 0 1 0 0 3 0 0 0 0 1 0 117 114 106 8 4 4 1 1 3 0 0 0 0 0 0 1 1 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccataagaagttactat tggagttggatacggcactccgccgggaagggactggagtggatcgggcgc atatatgacagtggtagtaca aactacaatccctccctcaagagtcgagtcagcatgtcagtggacacgtccaagaaccaggtctccctgaagttgatctctgtgaccgccgcggacacggccatgtattattgt gcgagagggaaggattactatgatacaactggctccctcgaggatggttttgatatt ARGKDYYDTTGSLEDGFDI IGHV4-4 IGHD3-22 IGHJ3 ggtggctccataagaagttactat tggagttggatacggcactccgccgggaagggactggagtggatcgggcgc atatatgacagtggtagtaca aactacaatccctccctcaagagtcgagtcagcatgtcagtggacacgtccaagaaccaggtctccctgaagttgatctctgtgaccgccgcggacacggccatgtattattgt gcgagagggaaggattactatgatacaactggctccctcgaggatggttttgatatt IGA1 23 210 12 11 8 17 4 6 8 6 6 3 JY8QFUQ01AF6U3 10684 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F or Homsap IGHV3-21*04 F 824 86.7 189/218 nt 86.24 188/218 nt Homsap IGHJ6*02 F (see comment) 193 79.03 49/62 nt Homsap IGHD6-19*01 F 1 8 8 17 8.8.17 [2.17.37.11] CVRDSDSSGYARYDSMDVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) in FR3-IMGT, from codon 100 of V-REGION: 2 nucleotides (from position 207 in the user submitted sequence), (cause frameshift) agcctctggattcaacctcaatacctttggcatgaactgggtccgccaggcgccagggaagggactggagtgggtctcacacgtcaatcggggtagtactcacatatactacgcaggctcagtgaggggccggttcaccatctccagagacgacgccgggaactcagtctatctgcaaatgaatagcctgagagccgaggacacgggtttattattgtgtgagagacagtgactcgtccggctacgcgaggtacgactctatggacgtctggggccaagggaccgcggtcaccgtctcatcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [301] c86>a,T29>N(- + -)|t88>c,F30>L(+ - -)|g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|a112>g,S38>G(+ + -)|t135>g|g147>a|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>a,S57>N(- - -)|a172>c,S58>R(- - -)|t174>g,S58>R(- - -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)|a206>g,D69>G(- - -)|a215>g,K72>R(+ + +)|a225>g|a244>g,N82>D(+ + -)|a250>g,K84>G(- - -)|a251>g,K84>G(- - -)|c259>g,L87>V(+ - +)|g261>c,L87>V(+ - +)|c276>t|g303>t|c309>t|c314>t,A105>V(+ - +)| c86>a,T29>N(- + -)|t88>c,F30>L(+ - -)|g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|a112>g,S38>G(+ + -)| t135>g|g147>a|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>a,S57>N(- - -)|a172>c,S58>R(- - -)|t174>g,S58>R(- - -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)| a206>g,D69>G(- - -)|a215>g,K72>R(+ + +)|a225>g|a244>g,N82>D(+ + -)|a250>g,K84>G(- - -)|a251>g,K84>G(- - -)|c259>g,L87>V(+ - +)|g261>c,L87>V(+ - +)|c276>t|g303>t|c309>t| c314>t,A105>V(+ - +)| 320 226 196 30 6 24 9 3 3 4 1 2 1 0 3 1 1 2 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 1 0 1 0 1 1 0 1 0 0 0 51 51 47 4 2 2 0 1 0 1 0 1 0 0 0 0 0 1 30 24 16 8 0 8 2 1 0 2 1 0 0 0 1 0 0 1 117 112 101 11 4 7 6 0 2 0 0 0 0 0 1 1 1 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct ggattcaacctcaatacctttggc atgaactgggtccgccaggcgccagggaagggactggagtgggtctcacac gtcaatcggggtagtactcacata tactacgcaggctcagtgaggggccggttcaccatctccagagacgacgccgggaactcagtctatctgcaaatgaatagcctgagagccgaggacacgggtttattattgt gtgagagacagtgactcgtccggctacgcgaggtacgactctatggacgtc VRDSDSSGYARYDSMDV IGHV3-21 IGHD6-19 IGHJ6 ggattcaacctcaatacctttggc atgaactgggtccgccaggcgccagggaagggactggagtgggtctcacac gtcaatcggggtagtactcacata tactacgcaggctcagtgaggggccggttcaccatctccagagacgacgccgggaactcagtctatctgcaaatgaatagcctgagagccgaggacacgggtttattattgt gtgagagacagtgactcgtccggctacgcgaggtacgactctatggacgtc IGG2 29 211 18 11 5 12 13 17 6 0 9 14 JY8QFUQ01AF7Y6 21165 productive (see comment) Homsap IGHV3-7*01 F 967 94.44 204/216 nt 93.98 203/216 nt Homsap IGHJ4*02 F 167 84.78 39/46 nt Homsap IGHD7-27*01 F 1 8 8 6 8.8.6 [1.17.37.11] CSRLLEEW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 66 of V-REGION: 1 nucleotides (from position 104 in the user submitted sequence), (cause frameshift) ctctggtttcacctttagtaacgattggatggactgggtccgccaggctccagggaaggggctggagtgggtggccaatataaagggagatggaagtgagaaaactatgtagactctgcgaagggccgattcatcatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgttcgagactccttgaggaatggggccagggaaccctggtcatcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagccctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 86 95 [266, 265] a81>t|g107>a,S36>N(- - -)|t109>g,Y37>D(- - -)|a118>g,S40>D(- - -)|g119>a,S40>D(- - -)|c165>t|c172>g,Q58>G(- - -)|a173>g,Q58>G(- - -)|g204>a|t212>c,V71>A(+ - +)|c230>t,T77>I(- - -)|c287>t,A96>V(+ - +)|g313>t,A105>S(- + -)| a81>t|g107>a,S36>N(- - -)|t109>g,Y37>D(- - -)| a118>g,S40>D(- - -)|g119>a,S40>D(- - -)|c165>t| c172>g,Q58>G(- - -)|a173>g,Q58>G(- - -)| g204>a|t212>c,V71>A(+ - +)|c230>t,T77>I(- - -)|c287>t,A96>V(+ - +)| g313>t,A105>S(- + -)|g319>c|a320>t| 318 (320) 222 (224) 209 13 (15) 3 (5) 10 2 3 3 1 0 0 1 (2) 0 0 (1) 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 0 0 0 0 1 0 0 0 0 1 51 51 48 3 1 2 1 1 1 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 1 0 0 117 113 109 4 1 3 0 1 2 1 0 0 0 0 0 0 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 0 0 0 0 0 0 (1) 0 0 (1) 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggtttcacctttagtaacgattgg atggactgggtccgccaggctccagggaaggggctggagtgggtggccaat ataaagggagatggaagtgagaaa actatgtagactctgcgaagggccgattcatcatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgt tcgagactccttgaggaa SRLLEE IGHV3-7 IGHD7-27 IGHJ4 ggtttcacctttagtaacgattgg atggactgggtccgccaggctccagggaaggggctggagtgggtggccaat ataaagggagatggaagtgagaaa actatgtagactctgcgaagggccgattcatcatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgt tcgagactccttgaggaa IGA2 12 212 9 3 6 7 3 5 2 1 5 4 JY8QFUQ01AF8I8 14487 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 935 91.07 204/224 nt NA Homsap IGHJ6*02 F (see comment) 202 80.65 50/62 nt Homsap IGHD1-14*01 ORF 1 8 10 17 8.10.17 [1.17.38.11] CTTAPSETYATEYFAMDVW in-frame + Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) cctctggattcactttcagtaatgtttggatgagttgggtccgtcaggctccagggaaggggctggaatgggttggccgtattaaaagcacaattgatggtgggacaacatcctacgctgcccccgtgaaagacagattcatcatctcacgagaggactcagaaaacaccttgtctctgcaaatgaacagcctgaaaaccgaggacacagccgtgtattactgtactacagccccaagcgaaacttatgcaacggagtacttcgctatggacgtctggggccaggggacaacggtcaccgtctcctcagcctccaccaaggggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG1 86 88 [308] c108>t|c110>t,A37>V(+ - +)|c111>t,A37>V(+ - +)|c120>t|c129>t|g153>a|a176>c,K59>T(- - -)|c179>t,T60>I(- - -)|g196>t,D66>S(- - -)|a197>c,D66>S(- - -)|a207>c|g221>a,G74>D(- - -)|c230>t,T77>I(- - -)|a238>c|t243>g,D81>E(+ - +)|t246>c|a250>g,K84>E(+ - -)|g258>c|c259>t|a263>c,Y88>S(+ - -)|c315>t| c108>t|c110>t,A37>V(+ - +)|c111>t,A37>V(+ - +)| c120>t|c129>t|g153>a| a176>c,K59>T(- - -)|c179>t,T60>I(- - -)| g196>t,D66>S(- - -)|a197>c,D66>S(- - -)|a207>c|g221>a,G74>D(- - -)|c230>t,T77>I(- - -)|a238>c|t243>g,D81>E(+ - +)|t246>c|a250>g,K84>E(+ - -)|g258>c|c259>t|a263>c,Y88>S(+ - -)| c315>t|a320>c| 319 (320) 231 (232) 210 21 (22) 10 (11) 11 1 2 9 1 5 (6) 0 0 0 1 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 3 0 0 0 0 0 0 0 0 0 51 51 48 3 3 0 0 1 2 0 0 0 0 0 0 0 0 0 30 30 28 2 0 2 0 0 1 0 1 0 0 0 0 0 0 0 117 114 102 12 5 7 1 1 2 1 4 0 0 0 1 0 1 1 7 (8) 7 6 1 (2) 1 (2) 0 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| cctct ggattcactttcagtaatgtttgg atgagttgggtccgtcaggctccagggaaggggctggaatgggttggccgt attaaaagcacaattgatggtgggacaaca tcctacgctgcccccgtgaaagacagattcatcatctcacgagaggactcagaaaacaccttgtctctgcaaatgaacagcctgaaaaccgaggacacagccgtgtattactgt actacagccccaagcgaaacttatgcaacggagtacttcgctatggacgtc TTAPSETYATEYFAMDV IGHV3-15 IGHD1-14 IGHJ6 ggattcactttcagtaatgtttgg atgagttgggtccgtcaggctccagggaaggggctggaatgggttggccgt attaaaagcacaattgatggtgggacaaca tcctacgctgcccccgtgaaagacagattcatcatctcacgagaggactcagaaaacaccttgtctctgcaaatgaacagcctgaaaaccgaggacacagccgtgtattactgt actacagccccaagcgaaacttatgcaacggagtacttcgctatggacgtc IGG1 20 219 12 8 10 12 2 8 8 1 7 4 JY8QFUQ01AF8ND 3484 productive Homsap IGHV4-61*02 F 974 93.67 207/221 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-19*01 F 1 10 7 13 10.7.13 [1.17.38.11] CARQGDSSAPWNDHW in-frame + tctctggtggctccatcagcagcggttattactactggagctggatccggcagcccgccggggagggactggagtgggttgggcgtatctctgccagtggggacaccaactacaacccctccctcaagagtcgagtcaccatatcagtgaacacgtccaagaaccagttctccttgaggctgacctctgtgaccgccgcagacacggccgtgtattactgtgcgagacaaggggacagtagtgctccctggaatgaccactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [292] t102>c|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a142>g,K48>E(+ - -)|a157>g,I53>V(+ - +)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a240>g|g241>a,D81>N(+ + -)|c265>t|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)| t102>c|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)| a142>g,K48>E(+ - -)|a157>g,I53>V(+ - +)| a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a240>g|g241>a,D81>N(+ + -)|c265>t|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)| g319>c| 318 (320) 227 (229) 213 (214) 14 (15) 3 (4) 11 6 3 1 1 1 0 1 0 1 (2) 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 1 2 0 1 0 1 0 0 1 0 0 0 0 0 51 51 49 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 30 21 17 4 0 4 2 1 0 0 1 0 0 0 0 0 0 0 117 114 109 5 2 3 2 1 1 0 0 0 0 0 1 0 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcagcggttattactac tggagctggatccggcagcccgccggggagggactggagtgggttgggcgt atctctgccagtggggacacc aactacaacccctccctcaagagtcgagtcaccatatcagtgaacacgtccaagaaccagttctccttgaggctgacctctgtgaccgccgcagacacggccgtgtattactgt gcgagacaaggggacagtagtgctccctggaatgaccac ARQGDSSAPWNDH IGHV4-61 IGHD6-19 IGHJ4 ggtggctccatcagcagcggttattactac tggagctggatccggcagcccgccggggagggactggagtgggttgggcgt atctctgccagtggggacacc aactacaacccctccctcaagagtcgagtcaccatatcagtgaacacgtccaagaaccagttctccttgaggctgacctctgtgaccgccgcagacacggccgtgtattactgt gcgagacaaggggacagtagtgctccctggaatgaccac IGG2 14 216 11 3 4 5 7 9 2 1 5 6 JY8QFUQ01AF8SZ 6971 productive (see comment) Homsap IGHV3-66*01 F, or Homsap IGHV3-66*02 F or Homsap IGHV3-66*04 F 823 87.04 188/216 nt 86.57 187/216 nt Homsap IGHJ1*01 F (see comment) 188 84.62 44/52 nt Homsap IGHD1-26*01 F 1 8 7 13 8.7.13 [2.17.38.11] CVGVGVGSSVSFQYW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 40 of V-REGION: 'G' (1 nucleotides at position 35 to 35 in the user submitted sequence), (cause frameshift); gcctctggattcactgtcaataacaactacatggGgttgggtccgccaggctccagggaaggggctggagtgggtctcgactatttattacggtggcaccacatattacgcagactccgcgaagggccgattcaccatctccagagacacctccaggaacacactttttcttcagatgaacagcctgagaagcgacgacacggctctatattattgtgtgggagtcggagtgggatcttcagtctccttccaatactggggccagggcacccttgtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] c87>t|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|a118>g,S40>G(+ + -)|c120>t,S40>G(+ + -)|a162>g|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)|a172>t,S58>Y(+ - -)|g173>a,S58>Y(+ - -)|t189>c|g191>c,S64>T(+ - +)|c198>t|t212>c,V71>A(+ - +)|a223>c|a245>c,N82>T(- + -)|t246>c,N82>T(- + -)|a251>g,K84>R(+ + +)|g258>a|g261>t|a263>t,Y88>F(- + -)|a270>g|g286>a,A96>S(- + -)|c287>g,A96>S(- + -)|g291>c,E97>D(+ - +)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)|c309>t|c314>t,A105>V(+ - +)|a316>g,R106>G(- - -)| c87>t|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)| a118>g,S40>G(+ + -)|c120>t,S40>G(+ + -)|a162>g|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)| a172>t,S58>Y(+ - -)|g173>a,S58>Y(+ - -)|t189>c|g191>c,S64>T(+ - +)| c198>t|t212>c,V71>A(+ - +)|a223>c|a245>c,N82>T(- + -)|t246>c,N82>T(- + -)|a251>g,K84>R(+ + +)|g258>a|g261>t|a263>t,Y88>F(- + -)|a270>g|g286>a,A96>S(- + -)|c287>g,A96>S(- + -)|g291>c,E97>D(+ - +)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)|c309>t| c314>t,A105>V(+ - +)|a316>g,R106>G(- - -)|a320>t| 319 (320) 223 (224) 193 30 (31) 9 (10) 21 5 7 5 4 2 0 2 (3) 0 3 1 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 2 1 0 0 0 0 0 0 0 0 0 51 51 46 5 1 4 2 1 1 1 0 0 0 0 0 0 0 0 30 21 17 4 1 3 0 1 0 1 0 0 1 0 1 0 0 0 117 114 98 16 6 10 2 3 2 2 2 0 1 0 2 1 1 0 7 (8) 7 5 2 (3) 0 (1) 2 1 0 1 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|ggca,220-223(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcactgtcaataacaactac atgggttgggtccgccaggctccagggaaggggctggagtgggtctcgact atttattacggtggcaccaca tattacgcagactccgcgaagggccgattcaccatctccagagacacctccaggaacacactttttcttcagatgaacagcctgagaagcgacgacacggctctatattattgt gtgggagtcggagtgggatcttcagtctccttccaatac VGVGVGSSVSFQY IGHV3-66 IGHD1-26 IGHJ1 ggattcactgtcaataacaactac atgggttgggtccgccaggctccagggaaggggctggagtgggtctcgact atttattacggtggcaccaca tattacgcagactccgcgaagggccgattcaccatctccagagacacctccaggaacacactttttcttcagatgaacagcctgagaagcgacgacacggctctatattattgt gtgggagtcggagtgggatcttcagtctccttccaatac IGA1 28 210 19 9 11 16 8 12 7 2 14 5 JY8QFUQ01AF9LD 18055 productive (see comment) Homsap IGHV3-7*01 F 950 93.12 203/218 nt 92.66 202/218 nt Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD3-10*01 F 3 8 8 13 8.8.13 [1.17.38.11] CARAIGRPFYGMDVW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 72 of V-REGION: 'A' (1 nucleotides at position 124 to 124 in the user submitted sequence), (cause frameshift); gctctggattctcctttgatatatattggatgagatgggtccgcctggctccagggaaggggctggagtgtgtggccgacataaagcaagatggaagtgagaagtactatgtggactctgtgaAagggccgattcaccatctccagagacaacgccaagaactcactgtttctgcaaatgaacagcctgagagtcgaggacacgggtgtgtatttctgtgcgagagcgatcggacggcccttctacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [289] c74>g|a85>t,T29>S(+ - +)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>t,S36>I(- - -)|c108>a,S36>I(- - -)|c120>a,S40>R(- - -)|a131>t,Q44>L(- - -)|g156>t,W52>C(+ - -)|a163>g,N55>D(+ + -)|a195>g|a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|c299>g,A100>G(- + -)|a308>t,Y103>F(- + -)| c74>g| a85>t,T29>S(+ - +)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>t,S36>I(- - -)|c108>a,S36>I(- - -)| c120>a,S40>R(- - -)|a131>t,Q44>L(- - -)|g156>t,W52>C(+ - -)|a163>g,N55>D(+ + -)| a195>g| a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|c299>g,A100>G(- + -)|a308>t,Y103>F(- + -)| a320>c| 319 (320) 225 (226) 210 15 (16) 2 (3) 13 3 1 1 0 0 (1) 2 4 0 0 2 2 0 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 19 5 0 5 1 1 0 0 0 1 1 0 0 0 1 0 51 51 47 4 0 4 1 0 0 0 0 1 1 0 0 0 1 0 30 24 23 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 117 114 110 4 0 4 0 0 1 0 0 0 2 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gctct ggattctcctttgatatatattgg atgagatgggtccgcctggctccagggaaggggctggagtgtgtggccgac ataaagcaagatggaagtgagaag tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtttctgcaaatgaacagcctgagagtcgaggacacgggtgtgtatttctgt gcgagagcgatcggacggcccttctacggtatggacgtc ARAIGRPFYGMDV IGHV3-7 IGHD3-10 IGHJ6 ggattctcctttgatatatattgg atgagatgggtccgcctggctccagggaaggggctggagtgtgtggccgac ataaagcaagatggaagtgagaag tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtttctgcaaatgaacagcctgagagtcgaggacacgggtgtgtatttctgt gcgagagcgatcggacggcccttctacggtatggacgtc IGA1 14 213 5 9 2 7 3 7 0 1 8 5 JY8QFUQ01AFBN6 4821 productive (see comment) Homsap IGHV4-61*01 F 1100 100 221/221 nt 99.55 220/221 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-12*01 F 3 10 7 20 10.7.20 [1.17.38.11] CARVSGYAYGRYGDYLRGVDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'C' (1 nucleotides at position 29 to 29 in the user submitted sequence), (cause frameshift); tctctggtggctccgtcagcagtggtagCttactactggagctggatccggcagcccccagggaagggactggagtggattgggtatatctattacagtgggagcaccaactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgtgcgagggttagtggctacgcgtacggacgctacggtgactatttgcgcggggttgactactggggccagggaaccctggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG4 100 88 [314] a318>g|a320>t| 317 (320) 226 (229) 226 (227) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcagcagtggtagttactac tggagctggatccggcagcccccagggaagggactggagtggattgggtat atctattacagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgt gcgagggttagtggctacgcgtacggacgctacggtgactatttgcgcggggttgactac ARVSGYAYGRYGDYLRGVDY IGHV4-61 IGHD5-12 IGHJ4 ggtggctccgtcagcagtggtagttactac tggagctggatccggcagcccccagggaagggactggagtggattgggtat atctattacagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgt gcgagggttagtggctacgcgtacggacgctacggtgactatttgcgcggggttgactac IGG4 0 216 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01AFCKU 13800 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 887 89.91 196/218 nt 89.45 195/218 nt Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD6-19*01 F 1 8 8 10 8.8.10 [1.17.38.11] CANEVGYCSGGG in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 59 of V-REGION: 'G' (1 nucleotides at position 91 to 91 in the user submitted sequence), (cause frameshift); cctctggattcacctttaacctctatgtcatgcactggttccgccaggctccagggaaggggctagagtgggtctcaactattcgtgccaGggggtgataggacacactacgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacgctgtttttgcaaatgtacagcctgagagtcgaggacacggccatatattactgtgcgaacgaagtcggatattgtagtggtgggggggcccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [280] g104>a,S35>N(- - -)|a106>c,S36>L(- - -)|g107>t,S36>L(- - -)|c113>t,A38>V(+ - +)|a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)|g124>t,V42>F(+ - -)|g150>a|g163>a,A55>T(- - -)|a169>c,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|t177>g,S59>R(- - -)|g188>a,G63>D(- - -)|c192>g,S64>R(- - -)|t196>c,Y66>H(+ - -)|g225>c|a263>t,Y88>F(- + -)|c265>t|a274>t,N92>Y(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|a318>c,K106>N(+ - -)| g104>a,S35>N(- - -)|a106>c,S36>L(- - -)|g107>t,S36>L(- - -)|c113>t,A38>V(+ - +)| a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)|g124>t,V42>F(+ - -)|g150>a|g163>a,A55>T(- - -)| a169>c,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|t177>g,S59>R(- - -)|g188>a,G63>D(- - -)|c192>g,S64>R(- - -)| t196>c,Y66>H(+ - -)|g225>c|a263>t,Y88>F(- + -)|c265>t|a274>t,N92>Y(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)| a318>c,K106>N(+ - -)| 320 226 203 23 3 20 0 6 3 2 4 0 2 0 2 1 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 1 0 1 0 0 0 0 0 1 0 51 51 46 5 1 4 0 3 0 0 1 0 0 0 0 0 1 0 30 24 18 6 0 6 0 1 0 1 1 0 0 0 1 1 0 1 117 114 107 7 2 5 0 1 2 1 0 0 2 0 1 0 0 0 8 8 7 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttaacctctatgtc atgcactggttccgccaggctccagggaaggggctagagtgggtctcaact attcgtgccaggggtgataggaca cactacgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacgctgtttttgcaaatgtacagcctgagagtcgaggacacggccatatattactgt gcgaacgaagtcggatattgtagtggtggg ANEVGYCSGG IGHV3-23 IGHD6-19 IGHJ4 ggattcacctttaacctctatgtc atgcactggttccgccaggctccagggaaggggctagagtgggtctcaact attcgtgccaggggtgataggaca cactacgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacgctgtttttgcaaatgtacagcctgagagtcgaggacacggccatatattactgt gcgaacgaagtcggatattgtagtggtggg IGA1 22 213 11 11 9 14 2 8 3 0 9 10 JY8QFUQ01AFCRV 6991 productive (see comment) Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 927 92.17 200/217 nt 91.71 199/217 nt Homsap IGHJ6*02 F (see comment) 184 77.42 48/62 nt Homsap IGHD6-25*01 F 2 8 8 14 8.8.14 [1.17.38.11] CAREVIPTATYGMDLW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*01 (shorter alignment but highest percentage of identity) in CDR1-IMGT at codon 36 of V-REGION: 'A' (1 nucleotides at position 22 to 22 in the user submitted sequence), (cause frameshift); gtctggattcacgtttagtagAttttgtcatccattgggtccgccagactccaggcaaggcgctggagtgggtggcagttatttggtatgatggaactaacaaatactatacagaatccgtgaagggccgattcaccatctccagagacaattccaagaacatgctgtatctgcaaatgaacagcctgagagccgaggacacggctgtttactactgtgcgagagaggtcataccaactgccacctacggcatggacctctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [292] c87>g|c90>t|c108>t|a110>t,Y37>F(- + -)|g113>t,G38>V(- - -)|g117>c,M39>I(+ + -)|c120>t|g133>a,A45>T(- - -)|g146>c,G49>A(- + -)|a168>t|g188>c,S63>T(+ - +)|t192>c|g202>a,A68>T(- - -)|c207>a,D69>E(+ - +)|c257>t,T86>M(- - -)|g303>t|t306>c| c87>g|c90>t|c108>t|a110>t,Y37>F(- + -)|g113>t,G38>V(- - -)| g117>c,M39>I(+ + -)|c120>t|g133>a,A45>T(- - -)|g146>c,G49>A(- + -)| a168>t|g188>c,S63>T(+ - +)|t192>c| g202>a,A68>T(- - -)|c207>a,D69>E(+ - +)|c257>t,T86>M(- - -)|g303>t|t306>c| 320 225 208 17 8 9 0 2 4 2 0 1 2 0 3 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 3 2 0 0 2 0 0 0 1 0 0 1 1 0 51 51 47 4 1 3 0 1 1 0 0 0 0 0 2 0 0 0 30 24 21 3 2 1 0 0 0 1 0 0 1 0 1 0 0 0 117 114 109 5 2 3 0 1 1 1 0 1 0 0 0 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcacgtttagtagttttgtc atccattgggtccgccagactccaggcaaggcgctggagtgggtggcagtt atttggtatgatggaactaacaaa tactatacagaatccgtgaagggccgattcaccatctccagagacaattccaagaacatgctgtatctgcaaatgaacagcctgagagccgaggacacggctgtttactactgt gcgagagaggtcataccaactgccacctacggcatggacctc AREVIPTATYGMDL IGHV3-33 IGHD6-25 IGHJ6 ggattcacgtttagtagttttgtc atccattgggtccgccagactccaggcaaggcgctggagtgggtggcagtt atttggtatgatggaactaacaaa tactatacagaatccgtgaagggccgattcaccatctccagagacaattccaagaacatgctgtatctgcaaatgaacagcctgagagccgaggacacggctgtttactactgt gcgagagaggtcataccaactgccacctacggcatggacctc IGG1 17 213 8 9 6 13 2 4 3 5 6 3 JY8QFUQ01AFDCM 5072 productive Homsap IGHV4-39*03 F 920 90.91 200/220 nt NA Homsap IGHJ6*02 F 256 90.32 56/62 nt Homsap IGHD1-14*01 ORF 2 10 7 12 10.7.12 [1.17.38.11] CTREGPYYYGMDVW in-frame + tctctggtgactccatcagtaatactgattactactgggtctggatccgccagaccccagggaagggactggagtggattgggagtatcgatttcagtgggagcacctactacaacccgtccctcaagagtcgagtcaccatatccatagacacgtccgagaaccggttctccctgaggttgacctctatgaccgccgcagacacggccgtctattactgtacgagagagggaccctactactacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatcccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccag NA IGA2 71 89 [289, 291] g83>a,G28>D(- - -)|c93>t|g101>a,S34>N(- - -)|g104>c,S35>T(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g119>t,G40>V(- - -)|c133>a,P45>T(+ + -)|g147>a|t169>g,Y57>D(- - -)|a173>t,Y58>F(- + -)|t174>c,Y58>F(- + -)|g238>a,V80>I(+ - +)|a250>g,K84>E(+ - -)|a257>g,Q86>R(+ - -)|a269>g,K90>R(+ + +)|c271>t|g275>c,S92>T(+ - +)|g280>a,V94>M(+ - -)|g303>c| g83>a,G28>D(- - -)|c93>t|g101>a,S34>N(- - -)|g104>c,S35>T(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g119>t,G40>V(- - -)|c133>a,P45>T(+ + -)|g147>a| t169>g,Y57>D(- - -)|a173>t,Y58>F(- + -)|t174>c,Y58>F(- + -)| g238>a,V80>I(+ - +)|a250>g,K84>E(+ - -)|a257>g,Q86>R(+ - -)|a269>g,K90>R(+ + +)|c271>t|g275>c,S92>T(+ - +)|g280>a,V94>M(+ - -)|g303>c| 311 220 200 20 4 16 4 6 2 1 0 1 1 0 3 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 1 5 1 3 1 0 0 0 0 0 1 0 0 0 51 51 48 3 1 2 0 1 0 0 0 1 0 0 0 0 1 0 30 21 18 3 0 3 0 0 0 1 0 0 1 0 0 0 0 1 116 114 105 8 2 6 3 2 1 0 0 0 0 0 2 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatcagtaatactgattactac tgggtctggatccgccagaccccagggaagggactggagtggattgggagt atcgatttcagtgggagcacc tactacaacccgtccctcaagagtcgagtcaccatatccatagacacgtccgagaaccggttctccctgaggttgacctctatgaccgccgcagacacggccgtctattactgt acgagagagggaccctactactacggtatggacgtc TREGPYYYGMDV IGHV4-39 IGHD1-14 IGHJ6 ggtgactccatcagtaatactgattactac tgggtctggatccgccagaccccagggaagggactggagtggattgggagt atcgatttcagtgggagcacc tactacaacccgtccctcaagagtcgagtcaccatatccatagacacgtccgagaaccggttctccctgaggttgacctctatgaccgccgcagacacggccgtctattactgt acgagagagggaccctactactacggtatggacgtc IGA2 20 216 13 7 8 13 5 7 3 1 8 8 JY8QFUQ01AFE1S 16457 productive (see comment) Homsap IGHV3-7*01 F, or Homsap IGHV3-7*02 F 945 93.09 202/217 nt 92.63 201/217 nt Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD3-10*01 F 3 8 8 13 8.8.13 [1.16.38.11] CARAIGRPFYGMDVW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 59 in the user submitted sequence), (cause frameshift) gctctggattctcctttgatatatattggatgagatgggtccgcctggctccagggaagggctggagtgtgtggccgacataaagcaagatggaagtgagaagtactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtttctgcaaatgaacagcctgagagtcgaggacacgggtgtgtatttctgtgcgagagcgatcggacggcccttctacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] c74>g|a85>t,T29>S(+ - +)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>t,S36>I(- - -)|c108>a,S36>I(- - -)|c120>a,S40>R(- - -)|a131>t,Q44>L(- - -)|g156>t,W52>C(+ - -)|a163>g,N55>D(+ + -)|a195>g|a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|c299>g,A100>G(- + -)|a308>t,Y103>F(- + -)| c74>g| a85>t,T29>S(+ - +)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>t,S36>I(- - -)|c108>a,S36>I(- - -)| c120>a,S40>R(- - -)|a131>t,Q44>L(- - -)|g156>t,W52>C(+ - -)|a163>g,N55>D(+ + -)| a195>g| a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|c299>g,A100>G(- + -)|a308>t,Y103>F(- + -)| a320>c| 319 (320) 224 (225) 209 15 (16) 2 (3) 13 3 1 1 0 0 (1) 2 4 0 0 2 2 0 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 19 5 0 5 1 1 0 0 0 1 1 0 0 0 1 0 51 50 46 4 0 4 1 0 0 0 0 1 1 0 0 0 1 0 30 24 23 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 117 114 110 4 0 4 0 0 1 0 0 0 2 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gctct ggattctcctttgatatatattgg atgagatgggtccgcctggctccagggaagggctggagtgtgtggccgac ataaagcaagatggaagtgagaag tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtttctgcaaatgaacagcctgagagtcgaggacacgggtgtgtatttctgt gcgagagcgatcggacggcccttctacggtatggacgtc ARAIGRPFYGMDV IGHV3-7 IGHD3-10 IGHJ6 ggattctcctttgatatatattgg atgagatgggtccgcctggctccagggaagggctggagtgtgtggccgac ataaagcaagatggaagtgagaag tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtttctgcaaatgaacagcctgagagtcgaggacacgggtgtgtatttctgt gcgagagcgatcggacggcccttctacggtatggacgtc IGA1 14 212 5 9 2 7 3 7 0 1 8 5 JY8QFUQ01AFE74 10168 productive Homsap IGHV3-9*02 F 896 90.37 197/218 nt NA Homsap IGHJ6*02 F 172 78.57 44/56 nt Homsap IGHD1-14*01 ORF 2 8 8 11 8.8.11 [1.17.38.11] CGKDMRPGGMDVW in-frame + cctccggattcacctctggaaagtatgccatgcactgggtccggcaagctccagggaaggacctggagtgggtctcaggcttgggtttggataatggtaggatagactacgcggactctgtgaagggccgattcaccatctccaaagacaacgccaagaattccctgtatctgcaaatgaacagcctgagagttgaggacacggccatgtattactgtggaaaagatatgcgaccgggcggtatggacgtctggggccaggggatcacggtcaccgtctcctcagcatccccggaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [283] t78>c|a104>g,D35>G(- - -)|t105>a,D35>G(- - -)|g106>a,D36>K(+ - -)|t108>g,D36>K(+ - -)|g146>a,G49>D(- - -)|t165>c|a166>t,I56>L(+ + +)|t168>g,I56>L(+ + +)|a169>g,S57>G(+ + -)|g173>t,W58>L(+ - -)|a175>g,N59>D(+ + -)|g185>a,S62>N(- - -)|c192>g,S64>R(- - -)|g197>a,G66>D(- - -)|t201>c|g239>a,R80>K(+ + +)|c255>t|t279>c|c287>t,A96>V(+ - +)|t301>a,L101>M(+ + -)|c314>g,A105>G(- + -)| t78>c| a104>g,D35>G(- - -)|t105>a,D35>G(- - -)|g106>a,D36>K(+ - -)|t108>g,D36>K(+ - -)| g146>a,G49>D(- - -)|t165>c| a166>t,I56>L(+ + +)|t168>g,I56>L(+ + +)|a169>g,S57>G(+ + -)|g173>t,W58>L(+ - -)|a175>g,N59>D(+ + -)|g185>a,S62>N(- - -)|c192>g,S64>R(- - -)| g197>a,G66>D(- - -)|t201>c|g239>a,R80>K(+ + +)|c255>t|t279>c|c287>t,A96>V(+ - +)|t301>a,L101>M(+ + -)| c314>g,A105>G(- + -)| 322 228 206 22 5 17 3 5 2 4 0 0 1 2 0 2 1 2 78 5 4 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 0 0 0 0 0 1 0 0 0 1 51 51 49 2 1 1 0 1 0 1 0 0 0 0 0 0 0 0 30 24 17 7 0 7 2 1 0 0 0 0 1 0 0 1 1 1 117 114 107 7 3 4 0 2 2 2 0 0 0 1 0 0 0 0 10 10 9 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctcc ggattcacctctggaaagtatgcc atgcactgggtccggcaagctccagggaaggacctggagtgggtctcaggc ttgggtttggataatggtaggata gactacgcggactctgtgaagggccgattcaccatctccaaagacaacgccaagaattccctgtatctgcaaatgaacagcctgagagttgaggacacggccatgtattactgt ggaaaagatatgcgaccgggcggtatggacgtc GKDMRPGGMDV IGHV3-9 IGHD1-14 IGHJ6 ggattcacctctggaaagtatgcc atgcactgggtccggcaagctccagggaaggacctggagtgggtctcaggc ttgggtttggataatggtaggata gactacgcggactctgtgaagggccgattcaccatctccaaagacaacgccaagaattccctgtatctgcaaatgaacagcctgagagttgaggacacggccatgtattactgt ggaaaagatatgcgaccgggcggtatggacgtc IGA1 20 213 13 7 7 9 6 11 4 0 5 11 JY8QFUQ01AFES7 8449 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 917 90.18 202/224 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-6*01 F 2 8 10 15 8.10.15 [1.17.38.11] CTTDRRGLSIPPQFDFW in-frame - cctctggattcactttcagtgacgcctggatgagctgggtccgccagattccagggaaggggctggagtgggttggccgtataaaaaacaaagctatgggtgagacaacagacttcgctgcacccgtgagaggcagattcagtatctcaagagatgattcaaaaaatacactgtatctgcacatgagtggcctgaaaaccgaggacacagccgtctattattgtaccacagatcggcggggtttgtctataccacctcaatttgacttctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [300, 299] a106>g,N36>D(+ + -)|g133>a,A45>I(+ - +)|c134>t,A45>I(+ - +)|t168>a|g173>a,S58>N(- - -)|a178>g,T60>A(- - -)|g181>a,D61>M(- - -)|a182>t,D61>M(- - -)|t183>g,D61>M(- - -)|g188>a,G63>E(- - -)|a200>t,Y67>F(- + -)|a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|c231>t,T77>S(+ - +)|c255>t|g258>a|a270>c,Q90>H(- + -)|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|a277>g,S93>G(+ + -)|g303>c|c309>t| a106>g,N36>D(+ + -)| g133>a,A45>I(+ - +)|c134>t,A45>I(+ - +)| t168>a|g173>a,S58>N(- - -)|a178>g,T60>A(- - -)|g181>a,D61>M(- - -)|a182>t,D61>M(- - -)|t183>g,D61>M(- - -)|g188>a,G63>E(- - -)| a200>t,Y67>F(- + -)|a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|c231>t,T77>S(+ - +)|c255>t|g258>a|a270>c,Q90>H(- + -)|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|a277>g,S93>G(+ + -)|g303>c|c309>t| 320 232 210 22 5 17 5 5 5 0 1 0 2 1 1 1 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 30 23 7 1 6 1 3 0 0 0 0 1 1 0 0 0 1 117 114 102 12 4 8 3 1 4 0 1 0 1 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| cctct ggattcactttcagtgacgcctgg atgagctgggtccgccagattccagggaaggggctggagtgggttggccgt ataaaaaacaaagctatgggtgagacaaca gacttcgctgcacccgtgagaggcagattcagtatctcaagagatgattcaaaaaatacactgtatctgcacatgagtggcctgaaaaccgaggacacagccgtctattattgt accacagatcggcggggtttgtctataccacctcaatttgacttc TTDRRGLSIPPQFDF IGHV3-15 IGHD6-6 IGHJ4 ggattcactttcagtgacgcctgg atgagctgggtccgccagattccagggaaggggctggagtgggttggccgt ataaaaaacaaagctatgggtgagacaaca gacttcgctgcacccgtgagaggcagattcagtatctcaagagatgattcaaaaaatacactgtatctgcacatgagtggcctgaaaaccgaggacacagccgtctattattgt accacagatcggcggggtttgtctataccacctcaatttgacttc IGA2 22 219 15 7 10 12 5 10 4 1 10 7 JY8QFUQ01AFGYG 17149 productive (see comment) Homsap IGHV3-30*01 F 932 92.2 201/218 nt 91.74 200/218 nt Homsap IGHJ4*02 F 240 100 48/48 nt Homsap IGHD3-10*02 F 1 8 8 16 8.8.16 [1.17.38.11] CVKNDLSWGTIEHYFDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 38 of V-REGION: 'G' (1 nucleotides at position 28 to 28 in the user submitted sequence), (cause frameshift); cctctggattcacgttcaacatttatgGgtctacactgggtccgccaggctccaggcaaggggctagagtgggtggcacacatatcatatgatggaaataagaaatactacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgacagctgaagacacggctatttattactgtgtgaaaaatgacctgtcttggggcacaatagagcactactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [298] c87>g|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>t,S36>I(- - -)|c108>t,S36>I(- - -)|c113>g,A38>G(- + -)|a115>c,M39>L(+ + -)|g117>a,M39>L(+ + -)|g163>c,V55>H(- + -)|t164>a,V55>H(- + -)|t165>c,V55>H(- + -)|g188>a,S63>N(- - -)|t192>g,N64>K(+ - -)|g284>c,R95>T(- - -)|g291>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|c314>t,A105>V(+ - +)| c87>g|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>t,S36>I(- - -)|c108>t,S36>I(- - -)|c113>g,A38>G(- + -)| a115>c,M39>L(+ + -)|g117>a,M39>L(+ + -)|g163>c,V55>H(- + -)|t164>a,V55>H(- + -)|t165>c,V55>H(- + -)| g188>a,S63>N(- - -)|t192>g,N64>K(+ - -)| g284>c,R95>T(- - -)|g291>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| c314>t,A105>V(+ - +)|g317>a,R106>K(+ + +)|g319>a| 316 (320) 222 (226) 204 (206) 18 (20) 2 (3) 16 (17) 0 5 (7) 2 2 1 0 0 1 2 2 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 1 5 0 1 1 1 0 0 0 0 0 2 1 0 51 51 46 5 0 5 0 1 0 1 1 0 0 1 1 0 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 117 114 110 4 1 3 0 2 0 0 0 0 0 0 1 0 1 0 4 (8) 4 3 (5) 1 (3) 0 (1) 1 (2) 0 0 (2) 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacgttcaacatttatggt ctacactgggtccgccaggctccaggcaaggggctagagtgggtggcacac atatcatatgatggaaataagaaa tactacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgacagctgaagacacggctatttattactgt gtgaaaaatgacctgtcttggggcacaatagagcactactttgactac VKNDLSWGTIEHYFDY IGHV3-30 IGHD3-10 IGHJ4 ggattcacgttcaacatttatggt ctacactgggtccgccaggctccaggcaaggggctagagtgggtggcacac atatcatatgatggaaataagaaa tactacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgacagctgaagacacggctatttattactgt gtgaaaaatgacctgtcttggggcacaatagagcactactttgactac IGA1 17 213 8 9 6 12 2 5 1 1 8 7 JY8QFUQ01AFJBJ 2360 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 945 93.09 202/217 nt NA Homsap IGHJ3*01 F 223 94 47/50 nt Homsap IGHD3-10*01 F 1 8 8 19 8.8.19 [1.17.38.11] CARPAFLWFGDLPAEDGFDVW in-frame + ttctggatacagctttaccaattactggatcggctgggtgcgccaggtgcccggaaaaggcctggagtggatggggagcatctatcctcgtgactctgacaccagatacagcccgtccttccaaggccaggtcaccttctcagccgacaagtccatcagtaccgcctaccttcagtggagcagtctagcgacctcggacaccgccatgtattactgtgcgagaccagcttttctctggttcggggacttaccggccgaggatggttttgatgtctggggccaaggaacaatggtcaccgtctcttcagcctccaccaagggcccatcggtcttccccctggcacctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactactttcccgaaccggtgacggtgtcgtggaactc NA IGG1 86 94 [306, 305] g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a133>g,M45>V(+ - -)|g141>a|t164>g,I55>S(- - -)|g175>c,G59>R(- - -)|t192>c|a232>t,I78>F(+ - -)|c255>t|g267>t|c279>t|g282>a|a283>g,K95>A(- - -)|a284>c,K95>A(- - -)|g286>a,A96>T(- - -)| g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a133>g,M45>V(+ - -)|g141>a|t164>g,I55>S(- - -)| g175>c,G59>R(- - -)|t192>c| a232>t,I78>F(+ - -)|c255>t|g267>t|c279>t|g282>a|a283>g,K95>A(- - -)|a284>c,K95>A(- - -)|g286>a,A96>T(- - -)| a320>c| 319 (320) 224 (225) 209 15 (16) 6 (7) 9 2 4 3 1 1 (2) 0 1 0 1 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 51 51 48 3 1 2 1 1 0 0 0 0 0 0 0 0 0 1 30 24 22 2 1 1 0 0 0 1 0 0 0 0 1 0 0 0 117 114 106 8 4 4 1 2 2 0 1 0 1 0 0 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ttct ggatacagctttaccaattactgg atcggctgggtgcgccaggtgcccggaaaaggcctggagtggatggggagc atctatcctcgtgactctgacacc agatacagcccgtccttccaaggccaggtcaccttctcagccgacaagtccatcagtaccgcctaccttcagtggagcagtctagcgacctcggacaccgccatgtattactgt gcgagaccagcttttctctggttcggggacttaccggccgaggatggttttgatgtc ARPAFLWFGDLPAEDGFDV IGHV5-51 IGHD3-10 IGHJ3 ggatacagctttaccaattactgg atcggctgggtgcgccaggtgcccggaaaaggcctggagtggatggggagc atctatcctcgtgactctgacacc agatacagcccgtccttccaaggccaggtcaccttctcagccgacaagtccatcagtaccgcctaccttcagtggagcagtctagcgacctcggacaccgccatgtattactgt gcgagaccagcttttctctggttcggggacttaccggccgaggatggttttgatgtc IGG1 15 213 10 5 7 9 3 6 5 1 6 3 JY8QFUQ01AFKEB 17729 productive Homsap IGHV1-8*02 F 822 87.38 187/214 nt NA Homsap IGHJ2*01 F 193 84.91 45/53 nt Homsap IGHD2-21*02 F 2 8 8 16 8.8.16 [0.17.38.11] CTTKFGSDWQSDWYFHVW in-frame + tggagacacattccccagccatgacatcaactgggtgcgacaggccactggagaagggcttgagtggatgggacggatgaaccctaagactggtgacacaagctttgcacagaagttccacgatagagtcaccatgatcagtgacacctccataagtacagtgtacatggagctgagtagcctgagatctgaagacacggccatttactattgtacgacaaaattcgggagtgactggcaatcggactggtacttccacgtctggggccgtggcacctcggtcaccgtctcctcagcatcccgaccagcccaaggtcttccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 79 [290] t82>g,Y28>D(- - -)|c87>a|a103>c,T35>P(+ + -)|t109>c,Y37>H(+ - -)|t114>c|c142>g,Q48>E(+ + -)|t163>c,W55>R(- - -)|c177>g,N59>K(+ - -)|g185>c,S62>T(+ - +)|a190>g,N64>D(+ + -)|g196>a,G66>S(+ + -)|a200>t,Y67>F(- + -)|g216>c,Q72>H(- + -)|g221>a,G74>D(- - -)|c222>t,G74>D(- - -)|c236>t,T79>I(- - -)|g240>t,R80>S(- - -)|a241>g,N81>D(+ + -)|c255>t|c260>t,A87>V(+ - +)|c261>g,A87>V(+ - +)|c276>t|g291>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|t306>c|c309>t|g313>a,A105>T(- - -)| t82>g,Y28>D(- - -)|c87>a|a103>c,T35>P(+ + -)|t109>c,Y37>H(+ - -)|t114>c| c142>g,Q48>E(+ + -)|t163>c,W55>R(- - -)| c177>g,N59>K(+ - -)|g185>c,S62>T(+ - +)|a190>g,N64>D(+ + -)| g196>a,G66>S(+ + -)|a200>t,Y67>F(- + -)|g216>c,Q72>H(- + -)|g221>a,G74>D(- - -)|c222>t,G74>D(- - -)|c236>t,T79>I(- - -)|g240>t,R80>S(- - -)|a241>g,N81>D(+ + -)|c255>t|c260>t,A87>V(+ - +)|c261>g,A87>V(+ - +)|c276>t|g291>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|t306>c|c309>t| g313>a,A105>T(- - -)|g317>c,R106>T(- - -)|g319>a|g320>a| 316 (320) 218 (222) 190 (191) 28 (31) 7 (9) 21 (22) 2 5 (7) 6 4 1 1 1 0 2 (3) 3 2 1 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 0 0 2 1 1 0 0 0 0 0 1 51 51 49 2 0 2 0 0 0 1 0 0 0 0 0 1 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 1 1 0 0 117 114 97 17 5 12 1 4 6 1 0 0 1 0 1 1 2 0 4 (8) 4 3 (4) 1 (4) 0 (2) 1 (2) 0 1 (3) 0 0 0 0 0 0 0 (1) 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,118-119(FR2)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|aa,241-242(FR3)|ta,251-252(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,251-252(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|aacc,169-172(CDR2)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggagacacattccccagccatgac atcaactgggtgcgacaggccactggagaagggcttgagtggatgggacgg atgaaccctaagactggtgacaca agctttgcacagaagttccacgatagagtcaccatgatcagtgacacctccataagtacagtgtacatggagctgagtagcctgagatctgaagacacggccatttactattgt acgacaaaattcgggagtgactggcaatcggactggtacttccacgtc TTKFGSDWQSDWYFHV IGHV1-8 IGHD2-21 IGHJ2 ggagacacattccccagccatgac atcaactgggtgcgacaggccactggagaagggcttgagtggatgggacgg atgaaccctaagactggtgacaca agctttgcacagaagttccacgatagagtcaccatgatcagtgacacctccataagtacagtgtacatggagctgagtagcctgagatctgaagacacggccatttactattgt acgacaaaattcgggagtgactggcaatcggactggtacttccacgtc IGA1 27 213 16 11 10 18 6 9 5 2 14 6 JY8QFUQ01AFSUH 12771 productive Homsap IGHV3-7*01 F 1040 97.71 213/218 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD4-23*01 ORF 2 8 8 12 8.8.12 [1.17.38.11] CVSSPYFYDSNGYW in-frame + cctctggattcaggtttagcatctattggatgagctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagcaagatggaagtgagaaatactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagcagcctgagagccgaggacacggctgtgtattactgtgtgagttcaccgtatttctatgatagtaatggttactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [285] c86>g,T29>R(- - -)|c87>g,T29>R(- - -)|t105>c|g107>t,S36>I(- - -)|a275>g,N92>S(- - -)|c314>t,A105>V(+ - +)| c86>g,T29>R(- - -)|c87>g,T29>R(- - -)|t105>c|g107>t,S36>I(- - -)| a275>g,N92>S(- - -)| c314>t,A105>V(+ - +)|a318>t,R106>S(- - -)|g319>t|a320>c| 317 (320) 223 (226) 217 6 (9) 1 (3) 5 (6) 1 0 1 1 0 (1) 0 0 (1) 0 0 2 1 (2) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 0 1 0 0 0 0 0 2 1 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 113 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 5 (8) 5 4 1 (4) 0 (2) 1 (2) 0 0 1 0 0 (1) 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaggtttagcatctattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaagtgagaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagcagcctgagagccgaggacacggctgtgtattactgt gtgagttcaccgtatttctatgatagtaatggttac VSSPYFYDSNGY IGHV3-7 IGHD4-23 IGHJ4 ggattcaggtttagcatctattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaagtgagaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagcagcctgagagccgaggacacggctgtgtattactgt gtgagttcaccgtatttctatgatagtaatggttac IGA1 5 213 2 3 0 3 2 2 0 1 1 3 JY8QFUQ01AFUZV 18218 productive Homsap IGHV3-7*01 F (see comment) 738 82.49 179/217 nt NA Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD2-15*01 F 1 8 8 15 8.8.15 [1.17.38.11] CARLKGLTHHDFYFDLW in-frame + Low V-REGION identity (82,49% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctggattcgtctttactaatcattggatgagttgggtccgccaggccacagggaaggggccggagtgggtggccaacatatccccagacggaaatacgaaatattttggggactctgtgaggggccgattcagcgtctccagagacaacggcaagcagtcatcgtatctggaaatgaataccctgacagtcgatgacacggctgtatacttctgtgcgaggttgaaggggctgacacatcacgacttctacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctgggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG2 100 100 [294] a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>c,Y37>H(+ - -)|c120>t|t135>c|c136>a,P46>T(+ + -)|t149>c,L50>P(- - -)|a169>t,K57>S(- - -)|a170>c,K57>S(- - -)|g171>c,K57>S(- - -)|a173>c,Q58>P(- - -)|t177>c|g188>a,S63>N(- - -)|g190>a,E64>T(- - -)|a191>c,E64>T(- - -)|c198>t|a200>t,Y67>F(- + -)|t203>g,V68>G(- - -)|a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|a232>g,I78>V(+ - +)|c248>g,A83>G(- + -)|a253>c,N85>Q(+ - +)|c255>g,N85>Q(+ - +)|c259>t,L87>S(- - -)|t260>c,L87>S(- - -)|c268>g,Q90>E(+ + -)|c276>t|g278>c,S93>T(+ - +)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)|g303>a|t306>c|a308>t,Y103>F(- + -)| a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>c,Y37>H(+ - -)| c120>t|t135>c|c136>a,P46>T(+ + -)|t149>c,L50>P(- - -)| a169>t,K57>S(- - -)|a170>c,K57>S(- - -)|g171>c,K57>S(- - -)|a173>c,Q58>P(- - -)|t177>c|g188>a,S63>N(- - -)|g190>a,E64>T(- - -)|a191>c,E64>T(- - -)| c198>t|a200>t,Y67>F(- + -)|t203>g,V68>G(- - -)|a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|a232>g,I78>V(+ - +)|c248>g,A83>G(- + -)|a253>c,N85>Q(+ - +)|c255>g,N85>Q(+ - +)|c259>t,L87>S(- - -)|t260>c,L87>S(- - -)|c268>g,Q90>E(+ + -)|c276>t|g278>c,S93>T(+ - +)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)|g303>a|t306>c|a308>t,Y103>F(- + -)| a318>g|g319>t|a320>t| 317 (320) 222 (225) 184 38 (41) 7 (10) 31 3 (4) 4 7 6 4 1 3 (4) 0 4 4 1 (2) 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 1 2 1 0 0 0 0 1 0 0 0 51 51 47 4 2 2 0 0 1 2 0 1 0 0 0 0 0 0 30 24 16 8 1 7 0 2 0 1 3 0 1 0 1 0 0 0 117 114 94 20 4 16 2 1 4 2 1 0 2 0 2 4 1 1 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcgtctttactaatcattgg atgagttgggtccgccaggccacagggaaggggccggagtgggtggccaac atatccccagacggaaatacgaaa tattttggggactctgtgaggggccgattcagcgtctccagagacaacggcaagcagtcatcgtatctggaaatgaataccctgacagtcgatgacacggctgtatacttctgt gcgaggttgaaggggctgacacatcacgacttctacttcgatctc ARLKGLTHHDFYFDL IGHV3-7 IGHD2-15 IGHJ2 ggattcgtctttactaatcattgg atgagttgggtccgccaggccacagggaaggggccggagtgggtggccaac atatccccagacggaaatacgaaa tattttggggactctgtgaggggccgattcagcgtctccagagacaacggcaagcagtcatcgtatctggaaatgaataccctgacagtcgatgacacggctgtatacttctgt gcgaggttgaaggggctgacacatcacgacttctacttcgatctc IGG2 38 213 20 18 11 21 9 17 6 1 18 13 JY8QFUQ01AFVJM 20364 productive (see comment) Homsap IGHV3-33*05 F (see comment) 761 83.49 182/218 nt 83.03 181/218 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-12*01 F 3 8 8 13 8.8.13 [1.17.38.11] CVKGRGYNDYGLDYW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (83,49% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR2-IMGT at codon 65 of V-REGION: 'G' (1 nucleotides at position 104 to 104 in the user submitted sequence), (cause frameshift); cgtctggattctccttcagcaattatgccatccactgggtccgccaggctccaggcaaggggctggagtgggtggcgaccatttcatatgatattaataaaaaGatattatgcagagtccgtgaggggccgattcaccctctccagagacaattccaagaacactctcgatctgctcatggatacccttcggttcgacgacacggctgtctattattgtgttaaaggacgtggatacaatgactacggcctcgactactggggcccgggaaccctgctcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [290] a85>t,T29>S(+ - +)|t105>c|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g113>c,G38>A(- + -)|g117>c,M39>I(+ + -)|a162>g|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)|t165>c,V55>T(- - -)|a168>t|g184>a,G62>I(- - -)|g185>t,G62>I(- - -)|a186>t,G62>I(- - -)|g188>a,S63>N(- - -)|t192>a,N64>K(+ - -)|c198>t|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|a232>c,I78>L(+ + +)|g258>t|g261>c|t262>g,Y88>D(- - -)|a269>t,Q90>L(- - -)|a270>c,Q90>L(- - -)|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|g278>c,S93>T(+ - +)|g282>t|a283>c|a285>g|g286>t,A96>F(+ - -)|c287>t,A96>F(+ - -)|g291>c,E97>D(+ - +)|g303>c|c309>t| a85>t,T29>S(+ - +)|t105>c|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g113>c,G38>A(- + -)| g117>c,M39>I(+ + -)|a162>g|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)|t165>c,V55>T(- - -)| a168>t|g184>a,G62>I(- - -)|g185>t,G62>I(- - -)|a186>t,G62>I(- - -)|g188>a,S63>N(- - -)|t192>a,N64>K(+ - -)| c198>t|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|a232>c,I78>L(+ + +)|g258>t|g261>c|t262>g,Y88>D(- - -)|a269>t,Q90>L(- - -)|a270>c,Q90>L(- - -)|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|g278>c,S93>T(+ - +)|g282>t|a283>c|a285>g|g286>t,A96>F(+ - -)|c287>t,A96>F(+ - -)|g291>c,E97>D(+ - +)|g303>c|c309>t| c314>t,A105>V(+ - +)|g315>t,A105>V(+ - +)|g317>a,R106>K(+ + +)|a320>g| 319 (320) 225 (226) 186 39 (40) 11 (12) 28 4 (5) 5 6 3 3 0 4 1 6 1 5 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 1 1 0 0 1 0 1 0 0 0 51 51 46 5 1 4 1 1 0 2 0 0 0 0 1 0 0 0 30 24 18 6 1 5 0 2 0 0 0 0 2 1 0 0 1 0 117 114 94 20 8 12 3 0 4 0 3 0 1 0 4 1 3 1 7 (8) 7 4 3 (4) 0 (1) 3 0 (1) 1 1 0 0 0 0 0 0 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cgtct ggattctccttcagcaattatgcc atccactgggtccgccaggctccaggcaaggggctggagtgggtggcgacc atttcatatgatattaataaaaaa tattatgcagagtccgtgaggggccgattcaccctctccagagacaattccaagaacactctcgatctgctcatggatacccttcggttcgacgacacggctgtctattattgt gttaaaggacgtggatacaatgactacggcctcgactac VKGRGYNDYGLDY IGHV3-33 IGHD5-12 IGHJ4 ggattctccttcagcaattatgcc atccactgggtccgccaggctccaggcaaggggctggagtgggtggcgacc atttcatatgatattaataaaaaa tattatgcagagtccgtgaggggccgattcaccctctccagagacaattccaagaacactctcgatctgctcatggatacccttcggttcgacgacacggctgtctattattgt gttaaaggacgtggatacaatgactacggcctcgactac IGG1 36 213 16 20 9 20 7 16 9 2 16 9 JY8QFUQ01AFVUY 17543 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 955 93.15 204/219 nt 92.69 203/219 nt Homsap IGHJ3*02 F 196 88 44/50 nt Homsap IGHD1-26*01 F 3 8 8 13 8.8.13 [2.17.38.11] CARFSGSYKSAFDIW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 59 to 59 in the user submitted sequence), (cause frameshift); ggttctggatacatctttaccgactattggatcggctgggtgcgccagacggccgggaAaaggcctggagtggatgggaatcatctatcctggtgactctgacaccagttatggcccgtccttccaaggccaggtcaccatttcagccgaccagtccatcaccaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatatattactgtgcgagattcagtgggagttacaaatcggcttttgatatctggggccgagggacaatggtctccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [290] g86>t,S29>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c111>t|t134>c,M45>T(- - -)|c136>g,P46>A(- - -)|g162>a|t192>c|a198>t,R66>S(- - -)|c201>t|a202>g,S68>G(+ + -)|c234>t|a244>c,K82>Q(+ - -)|g254>c,S85>T(+ - +)|g303>a,M101>I(+ + -)| g86>t,S29>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c111>t| t134>c,M45>T(- - -)|c136>g,P46>A(- - -)|g162>a| t192>c| a198>t,R66>S(- - -)|c201>t|a202>g,S68>G(+ + -)|c234>t|a244>c,K82>Q(+ - -)|g254>c,S85>T(+ - +)|g303>a,M101>I(+ + -)| c319>t|a320>t| 318 (320) 225 (227) 210 15 (17) 5 (7) 10 2 3 3 (4) 2 1 0 1 (2) 0 1 1 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 1 0 0 0 0 0 0 0 1 0 51 51 48 3 1 2 0 1 0 1 0 0 0 0 0 1 0 0 30 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 107 7 2 5 1 1 2 0 1 0 1 0 1 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ggttct ggatacatctttaccgactattgg atcggctgggtgcgccagacggccgggaaaggcctggagtggatgggaatc atctatcctggtgactctgacacc agttatggcccgtccttccaaggccaggtcaccatttcagccgaccagtccatcaccaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatatattactgt gcgagattcagtgggagttacaaatcggcttttgatatc ARFSGSYKSAFDI IGHV5-51 IGHD1-26 IGHJ3 ggatacatctttaccgactattgg atcggctgggtgcgccagacggccgggaaaggcctggagtggatgggaatc atctatcctggtgactctgacacc agttatggcccgtccttccaaggccaggtcaccatttcagccgaccagtccatcaccaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatatattactgt gcgagattcagtgggagttacaaatcggcttttgatatc IGA1 15 213 10 5 6 9 4 6 3 2 7 3 JY8QFUQ01AFZBJ 10026 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 818 86.98 187/215 nt 85.12 183/215 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-10*02 F 3 7 8 12 7.8.12 [1.17.38.11] CVKDISRSGCFEYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 38 of V-REGION: 'G' (1 nucleotides at position 24 to 24 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 52 of V-REGION: 'A' (1 nucleotides at position 68 to 68 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 67 of V-REGION: 'A' (1 nucleotides at position 108 to 108 in the user submitted sequence), (cause frameshift); in CDR1-IMGT, from codon 29 of V-REGION: 1 nucleotides (from position 10 in the user submitted sequence), (cause frameshift) tctggattccagttagcaactatGgccatgagctgggtccgtcaggctcctgggaaggggctggagtAgggtctcaactattagtaaagacggtgtttacacctactAaccccgactccgcgaagggccgggtcaccatctccagagacaattccaagaatacaatttatttgcaaatgaacagcctgacagccgaggacacggccagatattactgtgtgaaagacatttccaggtcaggctgctttgagtattggggtcagggaaccctgatcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [285] c87>a|t88>g,F30>V(+ - -)|g107>a,S36>N(- - -)|c129>t|a138>t|g163>a,A55>T(- - -)|g172>a,G58>K(- - -)|g173>a,G58>K(- - -)|t174>a,G58>K(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|t177>c,S59>D(- - -)|g188>t,G63>V(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a195>c|g202>c,A68>P(- - -)|a204>c,A68>P(- - -)|t212>c,V71>A(+ - +)|t226>g,F76>V(+ - -)|c255>t|g258>a|c259>a,L87>I(+ + +)|g261>t,L87>I(+ + +)|c265>t|g284>c,R95>T(- - -)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)|c314>t,A105>V(+ - +)| c87>a|t88>g,F30>V(+ - -)|g107>a,S36>N(- - -)| c129>t|a138>t|g163>a,A55>T(- - -)| g172>a,G58>K(- - -)|g173>a,G58>K(- - -)|t174>a,G58>K(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|t177>c,S59>D(- - -)|g188>t,G63>V(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a195>c| g202>c,A68>P(- - -)|a204>c,A68>P(- - -)|t212>c,V71>A(+ - +)|t226>g,F76>V(+ - -)|c255>t|g258>a|c259>a,L87>I(+ + +)|g261>t,L87>I(+ + +)|c265>t|g284>c,R95>T(- - -)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)| c314>t,A105>V(+ - +)| 320 223 194 29 7 22 1 8 4 2 2 2 2 1 2 0 2 3 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 20 3 1 2 0 1 0 0 0 1 0 0 0 0 0 1 51 51 48 3 2 1 0 1 1 0 0 0 1 0 0 0 0 0 30 24 14 10 1 9 1 4 0 1 1 0 1 1 0 0 1 0 117 114 102 12 3 9 0 2 2 1 1 1 0 0 2 0 1 2 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattccagttagcaactatgcc atgagctgggtccgtcaggctcctgggaaggggctggagtgggtctcaact attagtaaagacggtgtttacacc tactaccccgactccgcgaagggccgggtcaccatctccagagacaattccaagaatacaatttatttgcaaatgaacagcctgacagccgaggacacggccagatattactgt gtgaaagacatttccaggtcaggctgctttgagtat VKDISRSGCFEY IGHV3-23 IGHD3-10 IGHJ4 ggattccagttagcaactatgcc atgagctgggtccgtcaggctcctgggaaggggctggagtgggtctcaact attagtaaagacggtgtttacacc tactaccccgactccgcgaagggccgggtcaccatctccagagacaattccaagaatacaatttatttgcaaatgaacagcctgacagccgaggacacggccagatattactgt gtgaaagacatttccaggtcaggctgctttgagtat IGA2 28 212 14 14 11 17 3 11 5 2 10 11 JY8QFUQ01AG0D6 2960 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 833 87.16 190/218 nt NA Homsap IGHJ6*02 F, or Homsap IGHJ6*03 F (see comment) 175 75.81 47/62 nt Homsap IGHD4-17*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARLRSFGDYPLDVW in-frame - Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) cctctggattcaccttcaatctattcgggatgctctgggtccgccaacttccggggaagggactggtgtgggtctcacatattaatactgatggtactaaaatagatcacgcggactccgtgaagggccgattcaccacctccagagacaacgccaagaacaccctctatctgcaaatgaacagtctgagagccgaggacacggccgtatatttctgtgcgagactgcggtctttcggcgactaccccttagacgtctggggccaggggaccacggtcatcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [288] g104>a,S35>N(- - -)|a106>c,S36>L(- - -)|g107>t,S36>L(- - -)|c108>a,S36>L(- - -)|a110>t,Y37>F(- + -)|t112>g,W38>G(- - -)|a119>t,H40>L(- - -)|g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|a138>g|g147>a|g164>a,R55>H(- - +)|g173>c,S58>T(+ - +)|g186>t|g188>c,S63>T(+ - +)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|c194>t,T65>I(- - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|t233>c,I78>T(- - -)|g258>c|g261>c|t300>c|g303>a|a308>t,Y103>F(- + -)|a315>g| g104>a,S35>N(- - -)|a106>c,S36>L(- - -)|g107>t,S36>L(- - -)|c108>a,S36>L(- - -)|a110>t,Y37>F(- + -)|t112>g,W38>G(- - -)| a119>t,H40>L(- - -)|g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|a138>g|g147>a|g164>a,R55>H(- - +)| g173>c,S58>T(+ - +)|g186>t|g188>c,S63>T(+ - +)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|c194>t,T65>I(- - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|t233>c,I78>T(- - -)|g258>c|g261>c|t300>c|g303>a|a308>t,Y103>F(- + -)| a315>g|g319>c|a320>t| 318 (320) 224 (226) 195 29 (31) 8 (10) 21 3 6 3 3 1 2 3 (4) 0 5 (6) 0 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 1 0 0 1 1 1 0 0 0 1 1 51 51 45 6 2 4 1 2 1 0 0 0 1 0 1 0 0 0 30 24 18 6 1 5 0 1 1 0 0 1 0 0 2 0 1 0 117 114 104 10 4 6 1 2 1 3 0 0 1 0 2 0 0 0 6 (8) 6 5 1 (3) 1 (3) 0 1 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcaatctattcggg atgctctgggtccgccaacttccggggaagggactggtgtgggtctcacat attaatactgatggtactaaaata gatcacgcggactccgtgaagggccgattcaccacctccagagacaacgccaagaacaccctctatctgcaaatgaacagtctgagagccgaggacacggccgtatatttctgt gcgagactgcggtctttcggcgactaccccttagacgtc ARLRSFGDYPLDV IGHV3-74 IGHD4-17 IGHJ6 ggattcaccttcaatctattcggg atgctctgggtccgccaacttccggggaagggactggtgtgggtctcacat attaatactgatggtactaaaata gatcacgcggactccgtgaagggccgattcaccacctccagagacaacgccaagaacaccctctatctgcaaatgaacagtctgagagccgaggacacggccgtatatttctgt gcgagactgcggtctttcggcgactaccccttagacgtc IGA1 28 213 14 14 9 18 5 10 6 1 10 11 JY8QFUQ01AG2OG 1433 productive Homsap IGHV3-64*02 F (see comment) 769 84.26 182/216 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-9*01 F 2 8 8 16 8.8.16 [1.17.38.11] CVRGRPMAGFHGILYDNW in-frame + Low V-REGION identity (84,26% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tctggatttagtatcagtaattatggtattcactgggtccgccaggctccagggaggggactggaacttgtttcagctattaccaacaatgcgcatagtgtagtctatgtagactctgtgaagggcagattcatcatctccagagactattccaagaacacgttgtatcttcaaatgggcagactgagaccagaagacacggctgtgtactactgtgtcagagggcggcctatggctggctttcatggaatcctttatgacaactggggcctgggaacccttgtcaccgtctcctcagcatcccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [294] c84>t|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|t88>a,F30>I(+ - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|c113>g,A38>G(- + -)|g117>t,M39>I(+ + -)|a143>g,K48>R(+ + +)|t154>c,Y52>L(- - -)|a155>t,Y52>L(- - -)|g170>c,S57>T(+ - +)|t171>c,S57>T(+ - +)|g173>a,S58>N(- - -)|t174>c,S58>N(- - -)|g185>c,G62>A(- + -)|g187>c,G63>H(+ - -)|g188>a,G63>H(+ - -)|c192>t|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|t196>g,Y66>V(- - -)|a197>t,Y66>V(- - -)|t198>c,Y66>V(- - -)|c203>t,A68>V(+ - +)|c230>t,T77>I(- - -)|a244>t,N82>Y(- - -)|c259>t|c279>a,S93>R(- - -)|g286>c,A96>P(- - -)|t288>a,A96>P(- - -)|g291>a|t296>c,M99>T(- - -)|t306>c|c314>t,A105>V(+ - +)|g315>c,A105>V(+ - +)| c84>t|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|t88>a,F30>I(+ - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|c113>g,A38>G(- + -)| g117>t,M39>I(+ + -)|a143>g,K48>R(+ + +)|t154>c,Y52>L(- - -)|a155>t,Y52>L(- - -)| g170>c,S57>T(+ - +)|t171>c,S57>T(+ - +)|g173>a,S58>N(- - -)|t174>c,S58>N(- - -)|g185>c,G62>A(- + -)|g187>c,G63>H(+ - -)|g188>a,G63>H(+ - -)|c192>t|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)| t196>g,Y66>V(- - -)|a197>t,Y66>V(- - -)|t198>c,Y66>V(- - -)|c203>t,A68>V(+ - +)|c230>t,T77>I(- - -)|a244>t,N82>Y(- - -)|c259>t|c279>a,S93>R(- - -)|g286>c,A96>P(- - -)|t288>a,A96>P(- - -)|g291>a|t296>c,M99>T(- - -)|t306>c| c314>t,A105>V(+ - +)|g315>c,A105>V(+ - +)|a320>g| 319 (320) 223 (224) 187 36 (37) 5 (6) 31 2 (3) 4 9 6 0 1 3 2 5 2 1 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 1 6 0 1 3 0 0 0 0 1 0 2 0 0 51 51 47 4 0 4 1 0 0 1 0 0 1 0 0 0 1 0 30 24 14 10 1 9 1 2 2 2 0 0 0 0 3 0 0 0 117 114 101 13 3 10 0 1 3 3 0 1 2 1 1 0 0 1 7 (8) 7 5 2 (3) 0 (1) 2 0 (1) 0 1 0 0 0 0 0 1 0 0 0 aa,26-27(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|aa,152-153(FR2)|ta,154-155(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,154-155(FR2)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|tt,198-199(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|agct,162-165(FR2)|agta,169-172(CDR2)|ggta,187-190(CDR2)|ggca,220-223(FR3)|ggca,274-277(FR3)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|agct,162-165(FR2)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| tct ggatttagtatcagtaattatggt attcactgggtccgccaggctccagggaggggactggaacttgtttcagct attaccaacaatgcgcatagtgta gtctatgtagactctgtgaagggcagattcatcatctccagagactattccaagaacacgttgtatcttcaaatgggcagactgagaccagaagacacggctgtgtactactgt gtcagagggcggcctatggctggctttcatggaatcctttatgacaac VRGRPMAGFHGILYDN IGHV3-64 IGHD3-9 IGHJ4 ggatttagtatcagtaattatggt attcactgggtccgccaggctccagggaggggactggaacttgtttcagct attaccaacaatgcgcatagtgta gtctatgtagactctgtgaagggcagattcatcatctccagagactattccaagaacacgttgtatcttcaaatgggcagactgagaccagaagacacggctgtgtactactgt gtcagagggcggcctatggctggctttcatggaatcctttatgacaac IGA1 34 213 20 14 12 20 8 14 3 2 14 15 JY8QFUQ01AG5LX 14531 productive (see comment) Homsap IGHV3-74*03 F (see comment) 702 80.65 175/217 nt 80.18 174/217 nt Homsap IGHJ4*03 F 159 81.25 39/48 nt Homsap IGHD6-19*01 F 1 8 8 12 8.8.12 [1.17.38.11] CVRGTTGWRGVDYW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (80,65% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR2-IMGT at codon 41 of V-REGION: 'G' (1 nucleotides at position 36 to 36 in the user submitted sequence), (cause frameshift); ctctggattcaacgtcagtggatactggatgcactGgggtccgccaagtgccagggaaggggctggtatgggtctctaggctttcggatgatgaaattactataacttacgcggactttgtggagggccgattcaccatctccagagacaacgccaggaacgaggtctatctgcaaatgaatgatttgagagtcgacgatacggctgtatacttttgtgtgaggggaaccactggctggaggggggttgactactggggccaaggcgccctcgtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [285] c86>a,T29>N(- + -)|t88>g,F30>V(+ - -)|a106>g,S36>G(+ + -)|c108>a,S36>G(+ + -)|c134>t,A45>V(+ - +)|t135>g,A45>V(+ - +)|g153>a|a162>t|c163>a|t165>g|a166>c,I56>L(+ + +)|a169>t,N57>S(- - -)|a170>c,N57>S(- - -)|t171>g,N57>S(- - -)|a172>g,S58>D(- - -)|g173>a,S58>D(- - -)|g185>a,G62>E(- - -)|g186>a,G62>E(- - -)|g188>t,S63>I(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|c194>t,T65>I(- - -)|g198>t|c209>t,S70>F(- - -)|c210>t,S70>F(- - -)|a214>g,K72>E(+ - -)|a251>g,K84>R(+ + +)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|c259>g,L87>V(+ - +)|g261>c,L87>V(+ - +)|c276>t|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|c280>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c294>t|g303>a|t306>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| c86>a,T29>N(- + -)|t88>g,F30>V(+ - -)|a106>g,S36>G(+ + -)|c108>a,S36>G(+ + -)| c134>t,A45>V(+ - +)|t135>g,A45>V(+ - +)|g153>a|a162>t|c163>a|t165>g| a166>c,I56>L(+ + +)|a169>t,N57>S(- - -)|a170>c,N57>S(- - -)|t171>g,N57>S(- - -)|a172>g,S58>D(- - -)|g173>a,S58>D(- - -)|g185>a,G62>E(- - -)|g186>a,G62>E(- - -)|g188>t,S63>I(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|c194>t,T65>I(- - -)| g198>t|c209>t,S70>F(- - -)|c210>t,S70>F(- - -)|a214>g,K72>E(+ - -)|a251>g,K84>R(+ + +)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|c259>g,L87>V(+ - +)|g261>c,L87>V(+ - +)|c276>t|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|c280>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c294>t|g303>a|t306>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| c314>t,A105>V(+ - +)|a315>g,A105>V(+ - +)|a318>g|a320>g| 313 (320) 218 (225) 176 (179) 42 (46) 10 (12) 32 (34) 6 (9) 6 10 (11) 1 2 4 3 0 3 1 2 4 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 0 0 0 0 2 0 0 0 0 0 1 51 51 45 6 4 2 0 1 1 0 0 1 1 0 0 0 0 2 30 24 12 12 0 12 1 3 2 0 2 0 1 0 1 0 1 1 117 114 94 20 6 14 4 2 7 1 0 1 1 0 2 1 1 0 1 (8) 1 1 (4) 0 (4) 0 (2) 0 (2) 0 (3) 0 0 (1) 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaacgtcagtggatactgg atgcactgggtccgccaagtgccagggaaggggctggtatgggtctctagg ctttcggatgatgaaattactata acttacgcggactttgtggagggccgattcaccatctccagagacaacgccaggaacgaggtctatctgcaaatgaatgatttgagagtcgacgatacggctgtatacttttgt gtgaggggaaccactggctggaggggggttgactac VRGTTGWRGVDY IGHV3-74 IGHD6-19 IGHJ4 ggattcaacgtcagtggatactgg atgcactgggtccgccaagtgccagggaaggggctggtatgggtctctagg ctttcggatgatgaaattactata acttacgcggactttgtggagggccgattcaccatctccagagacaacgccaggaacgaggtctatctgcaaatgaatgatttgagagtcgacgatacggctgtatacttttgt gtgaggggaaccactggctggaggggggttgactac IGA2 42 213 23 19 16 26 7 16 10 0 16 16 JY8QFUQ01AG60V 8660 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 954 93.55 203/217 nt 93.09 202/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 3 8 8 13 8.8.13 [1.17.37.11] CARGLAVILGGFDYW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 84 of V-REGION: 1 nucleotides (from position 156 in the user submitted sequence), (cause frameshift) cctctggattcaccttcagtagttatagcatgcactgggtccgccaggctccagggaagggactggagtgggtctcctccattagtagtaatagtgcctacacagaccacgcagactcagtgaagggccgattcaccatctccagagacaacgacagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagggctagcagtgattcttggaggatttgactactggggccagggaaacctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [288] c108>t|a118>c,N40>H(- - -)|g147>a|a162>c|g176>a,S59>N(- - -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>c,S63>A(- + -)|t194>c,I65>T(- - -)|t196>g,Y66>D(- - -)|t199>c,Y67>H(+ - -)|c248>a,A83>D(- - -)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)| c108>t| a118>c,N40>H(- - -)|g147>a|a162>c| g176>a,S59>N(- - -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>c,S63>A(- + -)|t194>c,I65>T(- - -)| t196>g,Y66>D(- - -)|t199>c,Y67>H(+ - -)|c248>a,A83>D(- - -)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)| a320>g| 319 (320) 224 (225) 210 14 (15) 3 (4) 11 2 (3) 2 1 3 2 1 0 0 1 1 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 0 0 2 0 0 0 0 0 0 0 30 24 19 5 0 5 1 1 0 2 0 0 0 0 1 0 0 0 117 113 108 5 0 5 1 0 0 1 0 1 0 0 0 1 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtagttatagc atgcactgggtccgccaggctccagggaagggactggagtgggtctcctcc attagtagtaatagtgcctacaca gaccacgcagactcagtgaagggccgattcaccatctccagagacaacgacagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggctagcagtgattcttggaggatttgactac ARGLAVILGGFDY IGHV3-21 IGHD3-22 IGHJ4 ggattcaccttcagtagttatagc atgcactgggtccgccaggctccagggaagggactggagtgggtctcctcc attagtagtaatagtgcctacaca gaccacgcagactcagtgaagggccgattcaccatctccagagacaacgacagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggctagcagtgattcttggaggatttgactac IGG2 14 212 8 6 3 6 5 8 2 1 6 5 JY8QFUQ01AG93B 14951 productive (see comment) Homsap IGHV3-23*05 F 831 87.85 188/214 nt 86.92 186/214 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD1-14*01 ORF 1 7 7 10 7.7.10 [1.17.38.11] CAKRPPGTIDRW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 28 of V-REGION: 1 nucleotides (from position 10 in the user submitted sequence), (cause frameshift)in CDR2-IMGT, from codon 62 of V-REGION: 3 nucleotides (from position 94 in the user submitted sequence), (do not cause frameshift) cctctggatcacctttggcacctctgacatggcctgggtccgccaggttccaggggaggggctggagtgggtctcacacattgatatcagaggtgccacacagtataaagactccgtgaagggccggttcaccatctccagagacaattccaagagcactctatatctgcaaatgaacaccttgcgagccgaggacacggccgtatattactgtgcgaaacgcccgcccggcaccatagatcgctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [275] a103>g,S35>G(+ + -)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|c113>a,A38>D(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g163>c,A55>H(- - -)|c164>a,A55>H(- - -)|t165>c,A55>H(- - -)|t169>g,Y57>D(- - -)|g173>t,S58>I(- - -)|t177>a,S59>R(- - -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|t196>c,Y66>Q(- - -)|c198>g,Y66>Q(- - -)|g202>a,A68>K(- - -)|c203>a,A68>K(- - -)|a254>g,N85>S(- - -)|g258>t|g261>a|g278>c,S93>T(+ - +)|c280>t|a283>c| a103>g,S35>G(+ + -)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|c113>a,A38>D(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g163>c,A55>H(- - -)|c164>a,A55>H(- - -)|t165>c,A55>H(- - -)| t169>g,Y57>D(- - -)|g173>t,S58>I(- - -)|t177>a,S59>R(- - -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| t196>c,Y66>Q(- - -)|c198>g,Y66>Q(- - -)|g202>a,A68>K(- - -)|c203>a,A68>K(- - -)|a254>g,N85>S(- - -)|g258>t|g261>a|g278>c,S93>T(+ - +)|c280>t|a283>c| 318 220 194 26 4 22 5 2 2 2 2 3 0 1 5 1 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 19 4 0 4 1 0 0 0 1 1 0 0 1 0 0 0 51 51 44 7 0 7 2 0 1 1 0 1 0 0 2 0 0 0 30 21 16 5 0 5 1 0 0 0 0 0 0 1 1 0 1 1 117 114 104 10 4 6 1 2 1 1 1 1 0 0 1 1 1 0 6 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|agca,172-175(CDR2)|ggta,184-187(CDR2)|agca,190-193(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggatcacctttggcacctctgac atggcctgggtccgccaggttccaggggaggggctggagtgggtctcacac attgatatcagaggtgccaca cagtataaagactccgtgaagggccggttcaccatctccagagacaattccaagagcactctatatctgcaaatgaacaccttgcgagccgaggacacggccgtatattactgt gcgaaacgcccgcccggcaccatagatcgc AKRPPGTIDR IGHV3-23 IGHD1-14 IGHJ4 ggatcacctttggcacctctgac atggcctgggtccgccaggttccaggggaggggctggagtgggtctcacac attgatatcagaggtgccaca cagtataaagactccgtgaagggccggttcaccatctccagagacaattccaagagcactctatatctgcaaatgaacaccttgcgagccgaggacacggccgtatattactgt gcgaaacgcccgcccggcaccatagatcgc IGA2 26 209 11 15 4 15 7 11 4 0 13 9 JY8QFUQ01AG9DV 18013 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 855 88.48 192/217 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD5-18*01 F 3 8 8 21 8.8.21 [1.17.38.11] CAKDSRAGGYPNGYFYYSGLDVW in-frame - ctctggacgctccttgagaagctttggcatgcactgggtccgccaggctccaggcaagggactggagtgggtggcacttacttcgtatgacggaaataggaaatattatgcagactccgtgaagggccgattcaccatctccagagacaactccaagaatacgttatttctgcaaatggacagtctgagagctgaggacacggctctttattactgtgcgaaagattcgcgagcgggtggttatccaaatggatacttctattactctggtctggacgtctggggccaagggacaatagtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [312] t82>c,F28>R(- - -)|t83>g,F28>R(- - -)|a85>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|t105>a,S35>R(- - -)|a110>t,Y37>F(- + -)|g147>a|g163>c,V55>L(+ - +)|t167>c,I56>T(- - -)|a168>t,I56>T(- - -)|a171>g|t177>c|g188>a,S63>N(- - -)|a191>g,N64>R(+ - -)|t192>g,N64>R(+ - -)|c198>t|t246>c|c255>t|c259>t|g261>a|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)|g317>a,R106>K(+ + +)| t82>c,F28>R(- - -)|t83>g,F28>R(- - -)|a85>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|t105>a,S35>R(- - -)|a110>t,Y37>F(- + -)| g147>a|g163>c,V55>L(+ - +)| t167>c,I56>T(- - -)|a168>t,I56>T(- - -)|a171>g|t177>c|g188>a,S63>N(- - -)|a191>g,N64>R(+ - -)|t192>g,N64>R(+ - -)| c198>t|t246>c|c255>t|c259>t|g261>a|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)| g317>a,R106>K(+ + +)| 320 225 199 26 9 17 3 4 4 4 0 0 4 1 2 1 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 0 0 1 0 0 2 1 0 1 0 1 51 51 49 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 30 24 17 7 2 5 2 1 0 2 0 0 1 0 0 0 0 1 117 114 104 10 6 4 1 1 4 1 0 0 1 0 1 0 1 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggacgctccttgagaagctttggc atgcactgggtccgccaggctccaggcaagggactggagtgggtggcactt acttcgtatgacggaaataggaaa tattatgcagactccgtgaagggccgattcaccatctccagagacaactccaagaatacgttatttctgcaaatggacagtctgagagctgaggacacggctctttattactgt gcgaaagattcgcgagcgggtggttatccaaatggatacttctattactctggtctggacgtc AKDSRAGGYPNGYFYYSGLDV IGHV3-30 IGHD5-18 IGHJ6 ggacgctccttgagaagctttggc atgcactgggtccgccaggctccaggcaagggactggagtgggtggcactt acttcgtatgacggaaataggaaa tattatgcagactccgtgaagggccgattcaccatctccagagacaactccaagaatacgttatttctgcaaatggacagtctgagagctgaggacacggctctttattactgt gcgaaagattcgcgagcgggtggttatccaaatggatacttctattactctggtctggacgtc IGG2 25 213 14 11 7 11 7 14 7 2 5 11 JY8QFUQ01AGDKF 9532 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 891 90.32 196/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD6-13*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARRTITAAGRGDDYR in-frame - ctctgaattcacctttagcagttttgccatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaagcattggtactagtgttgttaacacatggtacgcagactcagtgaagggccggttcgccatttccagagacaattccaagagcacgctgtatttgcaaatgaatagcctgagagtcgaggacacggccgtatattactgtgcgaggaggacaataacagcagctggccgtggggatgactaccggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [290] g80>a,G27>E(- - -)|c108>t|a110>t,Y37>F(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t165>c,A55>S(- + -)|a169>g,S57>G(+ + -)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|g185>t,G62>V(- - -)|g188>t,G63>V(- - -)|g191>a,S64>N(- - -)|a197>g,Y66>W(- + -)|c198>g,Y66>W(- + -)|c210>a|a229>g,T77>A(- - -)|c234>t|a254>g,N85>S(- - -)|c265>t|c276>t|c287>t,A96>V(+ - +)| g80>a,G27>E(- - -)|c108>t|a110>t,Y37>F(- + -)| g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t165>c,A55>S(- + -)| a169>g,S57>G(+ + -)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|g185>t,G62>V(- - -)|g188>t,G63>V(- - -)|g191>a,S64>N(- - -)| a197>g,Y66>W(- + -)|c198>g,Y66>W(- + -)|c210>a|a229>g,T77>A(- - -)|c234>t|a254>g,N85>S(- - -)|c265>t|c276>t|c287>t,A96>V(+ - +)| a317>g,K106>R(+ + +)|a318>g,K106>R(+ + +)|g319>a|a320>g| 316 (320) 221 (225) 200 21 (25) 5 (7) 16 (18) 4 (7) 4 (5) 5 1 0 1 1 0 1 2 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 0 0 0 1 0 0 0 0 0 51 51 48 3 0 3 0 1 0 1 0 0 0 0 0 1 0 0 30 24 18 6 0 6 1 2 0 0 0 0 0 0 1 0 2 0 117 114 105 9 4 5 3 0 4 0 0 1 0 0 0 1 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (3) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gaattcacctttagcagttttgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaagc attggtactagtgttgttaacaca tggtacgcagactcagtgaagggccggttcgccatttccagagacaattccaagagcacgctgtatttgcaaatgaatagcctgagagtcgaggacacggccgtatattactgt gcgaggaggacaataacagcagctggccgtggggatgactac ARRTITAAGRGDDY IGHV3-23 IGHD6-13 IGHJ4 gaattcacctttagcagttttgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaagc attggtactagtgttgttaacaca tggtacgcagactcagtgaagggccggttcgccatttccagagacaattccaagagcacgctgtatttgcaaatgaatagcctgagagtcgaggacacggccgtatattactgt gcgaggaggacaataacagcagctggccgtggggatgactac IGA1 21 213 14 7 9 15 5 6 4 1 8 8 JY8QFUQ01AGDVH 11769 productive Homsap IGHV3-72*01 F 966 92.83 207/223 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-3*01 F 3 8 10 11 8.10.11 [1.17.38.11] CITSFGGRSLAYW in-frame + ctctggattcaccttaagtgatcactacatggactgggtccgccaggctccagggaaggggctggagtgggttggccgtactaaaaacaaagctaacggttacactacacactacgccgcgtctgtgagaggcagattcattctttcaagagacgattcaaagaactcagtgtatctgcaaatgaacagcctgaaaatcgaggacacggccgtctattactgtattacttcttttggtggaagatccctggcgtactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [288] c90>a,F30>L(+ - -)|c108>t|g170>a,R57>K(+ + +)|a184>g,S62>G(+ + -)|c192>t|g196>c,E66>H(- + -)|a198>c,E66>H(- + -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|c231>t,T77>I(- - -)|a232>c,I78>L(+ + +)|c234>t,I78>L(+ + +)|t243>c|c259>g,L87>V(+ - +)|c287>t,T96>I(- - -)|g303>c| c90>a,F30>L(+ - -)|c108>t| g170>a,R57>K(+ + +)|a184>g,S62>G(+ + -)|c192>t| g196>c,E66>H(- + -)|a198>c,E66>H(- + -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|c231>t,T77>I(- - -)|a232>c,I78>L(+ + +)|c234>t,I78>L(+ + +)|t243>c|c259>g,L87>V(+ - +)|c287>t,T96>I(- - -)|g303>c| g313>a,A105>I(+ - +)|c314>t,A105>I(+ - +)|g317>c,R106>T(- - -)|a318>t,R106>T(- - -)|g319>t|a320>c| 312 (320) 223 (231) 207 (209) 16 (22) 4 (6) 12 (16) 2 1 (2) 6 (7) 1 2 (3) 1 0 (1) 0 2 (3) 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 1 0 0 1 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 27 3 1 2 1 1 1 0 0 0 0 0 0 0 0 0 117 114 103 11 2 9 1 0 4 1 2 0 0 0 2 1 0 0 0 (8) 0 0 (2) 0 (6) 0 (2) 0 (4) 0 0 (1) 0 (1) 0 0 (1) 0 0 (1) 0 0 (1) 0 0 (1) 0 ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,165-168(FR2-CDR2)|agct,177-180(CDR2)|aact,253-256(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tgct,312-315(FR3)| ctct ggattcaccttaagtgatcactac atggactgggtccgccaggctccagggaaggggctggagtgggttggccgt actaaaaacaaagctaacggttacactaca cactacgccgcgtctgtgagaggcagattcattctttcaagagacgattcaaagaactcagtgtatctgcaaatgaacagcctgaaaatcgaggacacggccgtctattactgt attacttcttttggtggaagatccctggcgtac ITSFGGRSLAY IGHV3-72 IGHD3-3 IGHJ4 ggattcaccttaagtgatcactac atggactgggtccgccaggctccagggaaggggctggagtgggttggccgt actaaaaacaaagctaacggttacactaca cactacgccgcgtctgtgagaggcagattcattctttcaagagacgattcaaagaactcagtgtatctgcaaatgaacagcctgaaaatcgaggacacggccgtctattactgt attacttcttttggtggaagatccctggcgtac IGG2 16 219 10 6 7 11 3 5 2 2 9 3 JY8QFUQ01AGDVR 12450 productive Homsap IGHV3-30*01 F 932 92.2 201/218 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD5-24*01 ORF 1 8 8 16 8.8.16 [1.17.38.11] CAKDELPWDTIEYYFDYW in-frame + cctctggattcaccttcaatattttttctatgcactgggtccgccaggctccaggcaagggactagagtgggtgtcacttgtttcatatgatggatctaagaaaaagtacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtattactgtgcgaaagatgagctgccttgggacacaattgaatattattttgactattggggccagggaagcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtgtcatcgctgcctggtcagggcttcttccc NA IGA1 71 79 [297] g104>a,S35>N(- - -)|g107>t,S36>I(- - -)|c108>t,S36>I(- - -)|a110>t,Y37>F(- + -)|g112>t,A38>S(- + -)|g147>a|g160>t,A54>S(- + -)|g163>c,V55>L(+ - +)|a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|a187>t|g188>c|t192>g,N64>K(+ - -)|t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|c259>g,L87>V(+ - +)|g286>c,A96>P(- - -)|g317>a,R106>K(+ + +)| g104>a,S35>N(- - -)|g107>t,S36>I(- - -)|c108>t,S36>I(- - -)|a110>t,Y37>F(- + -)|g112>t,A38>S(- + -)| g147>a|g160>t,A54>S(- + -)|g163>c,V55>L(+ - +)| a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|a187>t|g188>c|t192>g,N64>K(+ - -)| t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|c259>g,L87>V(+ - +)|g286>c,A96>P(- - -)| g317>a,R106>K(+ + +)| 320 226 208 18 3 15 1 3 1 0 0 0 3 1 3 2 3 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 1 1 0 0 0 1 0 0 0 2 0 51 51 48 3 1 2 0 1 0 0 0 0 0 0 1 0 1 0 30 24 19 5 2 3 1 0 0 0 0 0 2 0 1 0 0 1 117 114 110 4 0 4 0 0 0 0 0 0 0 1 1 2 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcaatattttttct atgcactgggtccgccaggctccaggcaagggactagagtgggtgtcactt gtttcatatgatggatctaagaaa aagtacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtattactgt gcgaaagatgagctgccttgggacacaattgaatattattttgactat AKDELPWDTIEYYFDY IGHV3-30 IGHD5-24 IGHJ4 ggattcaccttcaatattttttct atgcactgggtccgccaggctccaggcaagggactagagtgggtgtcactt gtttcatatgatggatctaagaaa aagtacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtattactgt gcgaaagatgagctgccttgggacacaattgaatattattttgactat IGA1 17 213 4 13 3 11 1 6 1 2 6 8 JY8QFUQ01AGGIL 3530 productive Homsap IGHV3-7*01 F 933 91.82 202/220 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-21*02 F 2 8 8 18 8.8.18 [2.17.38.11] CSRHGGYTRLYNQNVYLDYW in-frame - ggcctctggattcacatttagcaacttttggatgagctgggtccgccagactccagggaaggggctggagtgggtggccaaaataaacccagacggaagtgagaaatactatgtggactctgtgaagggccgattcaccacctccagagacaactctagaaactcgctgtgtctgcaaatggacagcctgagagccgaggacacggctgtgtattactgttcgagacatggaggttacacgagactctacaatcaaaatgtctaccttgactactggggccagggaaccctggtcaccgtctccccagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [305] a72>g|c87>a|t105>c|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)|g133>a,A45>T(- - -)|c165>a,N55>K(+ - -)|g171>c,K57>N(+ - -)|a173>c,Q58>P(- - -)|t177>c|t233>c,I78>T(- - -)|g247>t,A83>S(- + -)|c249>t,A83>S(- + -)|a251>g,K84>R(+ + +)|g252>a,K84>R(+ + +)|a258>g|a263>g,Y88>C(- - -)|a274>g,N92>D(+ + -)|g313>t,A105>S(- + -)| a72>g| c87>a|t105>c|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)| g133>a,A45>T(- - -)|c165>a,N55>K(+ - -)| g171>c,K57>N(+ - -)|a173>c,Q58>P(- - -)|t177>c| t233>c,I78>T(- - -)|g247>t,A83>S(- + -)|c249>t,A83>S(- + -)|a251>g,K84>R(+ + +)|g252>a,K84>R(+ + +)|a258>g|a263>g,Y88>C(- - -)|a274>g,N92>D(+ + -)| g313>t,A105>S(- + -)|g319>c| 318 (320) 226 (228) 207 (208) 19 (20) 5 (6) 14 5 3 1 3 1 2 1 0 1 (2) 0 2 0 78 7 6 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 1 0 1 0 1 1 0 0 0 0 0 51 51 49 2 0 2 0 1 0 0 0 1 0 0 0 0 0 0 30 24 21 3 1 2 0 0 0 1 1 0 0 0 1 0 0 0 117 114 106 8 1 7 4 1 1 1 0 0 0 0 0 0 1 0 6 (8) 6 5 (6) 1 (2) 0 (1) 1 0 0 0 0 0 0 0 0 0 (1) 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ggcctct ggattcacatttagcaacttttgg atgagctgggtccgccagactccagggaaggggctggagtgggtggccaaa ataaacccagacggaagtgagaaa tactatgtggactctgtgaagggccgattcaccacctccagagacaactctagaaactcgctgtgtctgcaaatggacagcctgagagccgaggacacggctgtgtattactgt tcgagacatggaggttacacgagactctacaatcaaaatgtctaccttgactac SRHGGYTRLYNQNVYLDY IGHV3-7 IGHD2-21 IGHJ4 ggattcacatttagcaacttttgg atgagctgggtccgccagactccagggaaggggctggagtgggtggccaaa ataaacccagacggaagtgagaaa tactatgtggactctgtgaagggccgattcaccacctccagagacaactctagaaactcgctgtgtctgcaaatggacagcctgagagccgaggacacggctgtgtattactgt tcgagacatggaggttacacgagactctacaatcaaaatgtctaccttgactac IGA2 17 213 11 6 4 8 7 9 1 3 9 4 JY8QFUQ01AGHV3 10669 productive (see comment) Homsap IGHV4-61*02 F 874 89.04 195/219 nt 88.58 194/219 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-24*01 ORF 3 10 7 13 10.7.13 [1.17.37.11] CARDFGGSYNLVAYW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 68 of V-REGION: 1 nucleotides (from position 114 in the user submitted sequence), (cause frameshift) ctctggtgactccatcagtagtgattctcactactggagttggatccggcagcccgccgggaagggactggagtggattgggcgtgtctacgccagtgggaccaccaattacagccctccctcaagagtcgagtcaccatttcagtggacacgtccaggaatcaattctccctgaagttgaattctgtgaccgccgctgacacggccgtttatttctgtgcgagagattttggaggttcctacaatttagtggcctactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [290] g83>a,G28>D(- - -)|c93>t|g104>a,G35>D(- - -)|a106>t|g107>c|t109>c,Y37>H(+ - -)|c120>t|a166>g,I56>V(+ - +)|t171>c|a172>g,T58>A(- - -)|g191>c,S64>T(+ - +)|c198>t|c204>g|a234>t|a240>g|a251>g,K84>R(+ + +)|c255>t|g258>a|c271>t|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)|a291>t|g303>t|a308>t,Y103>F(- + -)| g83>a,G28>D(- - -)|c93>t|g104>a,G35>D(- - -)|a106>t|g107>c|t109>c,Y37>H(+ - -)| c120>t| a166>g,I56>V(+ - +)|t171>c|a172>g,T58>A(- - -)|g191>c,S64>T(+ - +)| c198>t|c204>g|a234>t|a240>g|a251>g,K84>R(+ + +)|c255>t|g258>a|c271>t|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)|a291>t|g303>t|a308>t,Y103>F(- + -)| 320 227 203 24 14 10 4 4 6 2 0 0 4 0 2 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 3 3 0 2 1 1 0 0 1 0 1 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 21 17 4 1 3 2 0 0 1 0 0 0 0 1 0 0 0 117 113 100 13 9 4 2 2 4 0 0 0 3 0 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtgactccatcagtagtgattctcactac tggagttggatccggcagcccgccgggaagggactggagtggattgggcgt gtctacgccagtgggaccacc aattacagccctccctcaagagtcgagtcaccatttcagtggacacgtccaggaatcaattctccctgaagttgaattctgtgaccgccgctgacacggccgtttatttctgt gcgagagattttggaggttcctacaatttagtggcctac ARDFGGSYNLVAY IGHV4-61 IGHD5-24 IGHJ4 ggtgactccatcagtagtgattctcactac tggagttggatccggcagcccgccgggaagggactggagtggattgggcgt gtctacgccagtgggaccacc aattacagccctccctcaagagtcgagtcaccatttcagtggacacgtccaggaatcaattctccctgaagttgaattctgtgaccgccgctgacacggccgtttatttctgt gcgagagattttggaggttcctacaatttagtggcctac IGG1 24 215 16 8 10 14 6 10 10 4 4 6 JY8QFUQ01AGKNE 20193 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 946 92.69 203/219 nt 92.24 202/219 nt Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [2.17.38.11] CTRDIGGRYSYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 47 of V-REGION: 'G' (1 nucleotides at position 56 to 56 in the user submitted sequence), (cause frameshift); gcctccggattcacctttagtagttactggatgcactgggtccgccaaactccagGgggagggactggtgtgggtctcacggactaatgaagatgggagtatcacaaactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgtacaagagacatcggtgggcggtactcctactggggccagggaatcctggtcaccgtctcctcagcatcccctgaccagccccaaggtcttcccgctgagcctcgacagcacccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 71 84 [282, 284] t78>c|c90>t|c108>t|g133>a,A45>T(- - -)|a142>g,K48>E(+ - -)|g147>a|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|g313>a,A105>T(- - -)| t78>c| c90>t|c108>t| g133>a,A45>T(- - -)|a142>g,K48>E(+ - -)|g147>a|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)| g313>a,A105>T(- - -)| 320 227 210 17 5 12 3 6 2 2 0 0 0 1 0 1 1 1 78 6 5 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 22 2 2 0 0 0 2 0 0 0 0 0 0 0 0 0 51 51 47 4 2 2 1 2 0 0 0 0 0 0 0 0 0 1 30 24 19 5 0 5 1 1 0 1 0 0 0 1 0 0 1 0 117 114 110 4 0 4 1 2 0 0 0 0 0 0 0 1 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctcc ggattcacctttagtagttactgg atgcactgggtccgccaaactccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt acaagagacatcggtgggcggtactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttagtagttactgg atgcactgggtccgccaaactccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt acaagagacatcggtgggcggtactcctac IGA2 15 213 11 4 7 9 4 6 2 2 6 5 JY8QFUQ01AGKQB 7043 productive Homsap IGHV3-7*01 F 990 95.39 207/217 nt NA Homsap IGHJ4*02 F 69 60.42 29/48 nt Homsap IGHD2-15*01 F 2 8 8 12 8.8.12 [1.17.38.11] CAKGGGNGDLDYWG in-frame + ctctggattcacctttagtaaccattggatgaactgggtccgccaggctccagggaaggggctggagtgggtggccaacataatgccagatggaggtgagaaattctatgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgaaggggggtggtaacggcgaccttgactactgggggccaggggaaccctgggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagnacccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 79 89 [284, 285] g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)|g119>a,S40>N(- - -)|a170>t,K57>M(- + -)|a173>c,Q58>P(- - -)|a187>g,S63>G(+ + -)|a197>t,Y66>F(- + -)|a225>g|a245>g,N82>S(- - -)|c265>t| g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)| g119>a,S40>N(- - -)| a170>t,K57>M(- + -)|a173>c,Q58>P(- - -)|a187>g,S63>G(+ + -)| a197>t,Y66>F(- + -)|a225>g|a245>g,N82>S(- - -)|c265>t| g317>a,R106>K(+ + +)|a318>g,R106>K(+ + +)|a320>g| 316 (320) 221 (225) 211 (212) 10 (13) 2 (3) 8 (10) 3 (5) 2 (3) 1 1 1 0 2 0 0 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 0 0 1 0 1 0 0 0 0 0 117 114 110 4 2 2 2 0 1 0 0 0 1 0 0 0 0 0 4 (8) 4 4 (5) 0 (3) 0 (1) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtaaccattgg atgaactgggtccgccaggctccagggaaggggctggagtgggtggccaac ataatgccagatggaggtgagaaa ttctatgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgaaggggggtggtaacggcgaccttgactactgg AKGGGNGDLDYW IGHV3-7 IGHD2-15 IGHJ4 ggattcacctttagtaaccattgg atgaactgggtccgccaggctccagggaaggggctggagtgggtggccaac ataatgccagatggaggtgagaaa ttctatgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgaaggggggtggtaacggcgaccttgactactgg IGA2 10 213 7 3 3 3 4 7 2 0 3 5 JY8QFUQ01AGMKR 13688 productive Homsap IGHV4-4*01 F, or Homsap IGHV4-4*02 F or Homsap IGHV4-4*03 F or Homsap IGHV4-4*04 F or Homsap IGHV4-4*05 F 873 89.4 194/217 nt NA Homsap IGHJ3*02 F 187 86 43/50 nt Homsap IGHD6-13*01 F 2 9 7 14 9.7.14 [1.17.38.11] CARDKDVTARGTFDMW in-frame + ctctggtgtctccatcagtagtaattactggtggatttgggtccgccagtccccaggggaggggctggagtggattggagaagtctatcatactgggaccacctattacaacccgtccctgaagagtcgagtcaccctgtcagtagacaagtccaggaatcagttctccctggagatgacttctgtgaccgccgcggacacggccgtgtatttctgtgcgagagataaagacgtgacagctcgtgggacctttgatatgtggggccaggggacagtggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [290] g83>t,G28>V(- - -)|c93>t|g107>a,S36>N(- - -)|a109>t,N37>Y(- - -)|g119>t,S40>I(- - -)|c133>t,P45>S(+ - -)|a142>g,K48>E(+ - -)|g162>a|a166>g,I56>V(+ - +)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)|a196>t,N66>Y(- - -)|c198>t,N66>Y(- - -)|c213>g|a232>c,I78>L(+ + +)|a234>g,I78>L(+ + +)|a251>g,K84>R(+ + +)|c255>t|a268>g,K90>E(+ - -)|c271>a,L91>M(+ + -)|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g308>t,C103>F(+ - -)| g83>t,G28>V(- - -)|c93>t|g107>a,S36>N(- - -)|a109>t,N37>Y(- - -)| g119>t,S40>I(- - -)|c133>t,P45>S(+ - -)|a142>g,K48>E(+ - -)|g162>a| a166>g,I56>V(+ - +)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)| a196>t,N66>Y(- - -)|c198>t,N66>Y(- - -)|c213>g|a232>c,I78>L(+ + +)|a234>g,I78>L(+ + +)|a251>g,K84>R(+ + +)|c255>t|a268>g,K90>E(+ - -)|c271>a,L91>M(+ + -)|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g308>t,C103>F(+ - -)| 320 225 202 23 4 19 5 2 5 0 1 1 2 0 3 1 3 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 27 23 4 1 3 0 1 1 0 0 0 1 0 0 0 1 0 51 51 47 4 1 3 1 1 1 0 0 0 0 0 0 0 1 0 30 21 18 3 0 3 1 0 0 0 0 0 0 0 2 0 0 0 117 114 102 12 2 10 3 0 3 0 1 1 1 0 1 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)| ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tgct,307-310(FR3)| ctct ggtgtctccatcagtagtaattactgg tggatttgggtccgccagtccccaggggaggggctggagtggattggagaa gtctatcatactgggaccacc tattacaacccgtccctgaagagtcgagtcaccctgtcagtagacaagtccaggaatcagttctccctggagatgacttctgtgaccgccgcggacacggccgtgtatttctgt gcgagagataaagacgtgacagctcgtgggacctttgatatg ARDKDVTARGTFDM IGHV4-4 IGHD6-13 IGHJ3 ggtgtctccatcagtagtaattactgg tggatttgggtccgccagtccccaggggaggggctggagtggattggagaa gtctatcatactgggaccacc tattacaacccgtccctgaagagtcgagtcaccctgtcagtagacaagtccaggaatcagttctccctggagatgacttctgtgaccgccgcggacacggccgtgtatttctgt gcgagagataaagacgtgacagctcgtgggacctttgatatg IGA1 23 213 12 11 7 15 5 8 3 1 13 6 JY8QFUQ01AGQWQ 9396 productive (see comment) Homsap IGHV3-69-1*02 P 920 92.92 197/212 nt 92.45 196/212 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD1-20*01 F 3 8 7 10 8.7.10 [1.16.38.11] CARDDKWAFDYW in-frame + Potentially, because of detected insertion/deletion however the closest V is a pseudogene in FR2-IMGT, from codon 55 of V-REGION: 3 nucleotides (from position 78 in the user submitted sequence), (do not cause frameshift) tctctggattcaccttcagtgactacagcatgaactgggtccgccaggctccagggcaggggctggagtgggtctcatatagtcgcggaagaaccacatactacgcagactctgtgcagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgacagccgaggacacggctgtttattactgtgcgagagatgataagtgggcatttgactactggggccagggaaccctggtcgtcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaaggatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 71 89 [273, 275] c74>t|t112>a,Y38>S(+ - -)|a113>g,Y38>S(+ - -)|a142>c,K48>Q(+ - -)|a166>t,I56>Y(- - -)|t167>a,I56>Y(- - -)|a172>c,S58>R(- - -)|t174>c,S58>R(- - -)|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|t189>a,S63>R(- - -)|t194>c,I65>T(- - -)|a214>c,K72>Q(+ - -)|c246>t|g284>c,R95>T(- - -)| c74>t| t112>a,Y38>S(+ - -)|a113>g,Y38>S(+ - -)| a142>c,K48>Q(+ - -)| a166>t,I56>Y(- - -)|t167>a,I56>Y(- - -)|a172>c,S58>R(- - -)|t174>c,S58>R(- - -)|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|t189>a,S63>R(- - -)|t194>c,I65>T(- - -)| a214>c,K72>Q(+ - -)|c246>t|g284>c,R95>T(- - -)| 320 220 205 15 2 13 2 0 2 2 3 0 1 4 1 0 0 0 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 1 0 0 0 0 51 48 47 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 30 21 13 8 0 8 1 0 0 2 1 0 1 3 0 0 0 0 117 114 111 3 1 2 0 0 1 0 1 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tctct ggattcaccttcagtgactacagc atgaactgggtccgccaggctccagggcaggggctggagtgggtctca tatagtcgcggaagaaccaca tactacgcagactctgtgcagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgacagccgaggacacggctgtttattactgt gcgagagatgataagtgggcatttgactac ARDDKWAFDY IGHV3-69-1 IGHD1-20 IGHJ4 ggattcaccttcagtgactacagc atgaactgggtccgccaggctccagggcaggggctggagtgggtctca tatagtcgcggaagaaccaca tactacgcagactctgtgcagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgacagccgaggacacggctgtttattactgt gcgagagatgataagtgggcatttgactac IGA2 14 207 5 9 1 2 4 12 1 0 3 10 JY8QFUQ01AGRBK 5029 productive (see comment) Homsap IGHV3-7*01 F, or Homsap IGHV3-7*02 F 931 92.59 200/216 nt 92.13 199/216 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-19*01 F 1 8 8 14 8.8.14 [1.16.38.11] CATDDSNGWDEVADYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 58 in the user submitted sequence), (cause frameshift) ctctggattcaccttcagctcccattggatgagctgggtccgccagactccagggaagggctggagtgggtggccaacataaaggaagatggaagtgtgaagtattatgtggactctgtgaggggccgattcaccatctccagagacaacgccaagaattcattgtatctgcagatgaacagcctgagaggcgaggacacggctgtctattactgtgcgacagatgatagtaatggctgggacgaggtcgctgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [289] t90>c|t105>c|a106>t|g107>c|t109>c,Y37>H(+ - -)|g133>a,A45>T(- - -)|c172>g,Q58>E(+ + -)|a191>t,E64>V(- + -)|a195>g|c198>t|a215>g,K72>R(+ + +)|c255>t|c259>t|a270>g|c287>g,A96>G(- + -)|g303>c|g317>c,R106>T(- - -)| t90>c|t105>c|a106>t|g107>c|t109>c,Y37>H(+ - -)| g133>a,A45>T(- - -)| c172>g,Q58>E(+ + -)|a191>t,E64>V(- + -)|a195>g| c198>t|a215>g,K72>R(+ + +)|c255>t|c259>t|a270>g|c287>g,A96>G(- + -)|g303>c| g317>c,R106>T(- - -)| 320 224 207 17 10 7 3 1 3 3 0 0 2 0 3 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 4 1 0 0 0 3 0 0 1 0 1 0 0 0 51 50 49 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 1 0 0 0 0 0 1 0 0 1 0 0 117 114 107 7 5 2 2 0 3 0 0 0 0 0 1 1 0 0 8 8 7 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagctcccattgg atgagctgggtccgccagactccagggaagggctggagtgggtggccaac ataaaggaagatggaagtgtgaag tattatgtggactctgtgaggggccgattcaccatctccagagacaacgccaagaattcattgtatctgcagatgaacagcctgagaggcgaggacacggctgtctattactgt gcgacagatgatagtaatggctgggacgaggtcgctgactac ATDDSNGWDEVADY IGHV3-7 IGHD6-19 IGHJ4 ggattcaccttcagctcccattgg atgagctgggtccgccagactccagggaagggctggagtgggtggccaac ataaaggaagatggaagtgtgaag tattatgtggactctgtgaggggccgattcaccatctccagagacaacgccaagaattcattgtatctgcagatgaacagcctgagaggcgaggacacggctgtctattactgt gcgacagatgatagtaatggctgggacgaggtcgctgactac IGA1 16 212 10 6 4 8 6 8 5 5 3 3 JY8QFUQ01AGS4P 21641 productive Homsap IGHV3-30*01 F, or Homsap IGHV3-30*11 F 850 88.43 191/216 nt NA Homsap IGHJ6*02 F (see comment) 184 77.42 48/62 nt Homsap IGHD3-10*01 F 3 8 8 18 8.8.18 [1.17.38.11] CARGTMLRGVTKARYGLDVW in-frame - Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) tctggattcaccttcagtacctatgctatgtactggatccgccaggctccaggcaaggggccagagtgggtgtcagtgatatcacatgatggaaataaggaagaatacgcagactccgtgaagggccgattcaccatttccagagacaactccaagaaaatgttgtacctgcaaatgaacaaccagcgacctgatgacacggctgtttattattgtgcgagagggactatgcttcggggagtcaccaaagcgagatacggtctggacgtctggggccaagggaccacggtcatcgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [302] g107>c,S36>T(+ - +)|c118>t,H40>Y(+ - -)|g124>a,V42>I(+ - +)|t149>c,L50>P(- - -)|g160>t,A54>S(- + -)|t165>g|t172>c,Y58>H(+ - -)|g188>a,S63>N(- - -)|t192>g,N64>K(+ - -)|a193>g,K65>E(+ - -)|t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|c234>t|t246>c|c255>a,N85>K(+ - -)|c257>t,T86>M(- - -)|c259>t|t264>c|g278>a,S93>N(- - -)|t281>a,L94>Q(- - -)|a283>c|g286>c,A96>P(- - -)|g291>t,E97>D(+ - +)|g303>t|c309>t| g107>c,S36>T(+ - +)| c118>t,H40>Y(+ - -)|g124>a,V42>I(+ - +)|t149>c,L50>P(- - -)|g160>t,A54>S(- + -)|t165>g| t172>c,Y58>H(+ - -)|g188>a,S63>N(- - -)|t192>g,N64>K(+ - -)|a193>g,K65>E(+ - -)| t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|c234>t|t246>c|c255>a,N85>K(+ - -)|c257>t,T86>M(- - -)|c259>t|t264>c|g278>a,S93>N(- - -)|t281>a,L94>Q(- - -)|a283>c|g286>c,A96>P(- - -)|g291>t,E97>D(+ - +)|g303>t|c309>t| a320>g| 319 (320) 223 (224) 198 25 (26) 8 (9) 17 1 (2) 3 5 4 1 2 0 1 2 0 3 3 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 46 5 1 4 0 1 1 1 0 0 0 0 0 0 1 1 30 24 20 4 0 4 1 1 0 1 0 0 0 0 0 0 0 1 117 114 99 15 7 8 0 1 4 2 1 2 0 1 1 0 2 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcagtacctatgct atgtactggatccgccaggctccaggcaaggggccagagtgggtgtcagtg atatcacatgatggaaataaggaa gaatacgcagactccgtgaagggccgattcaccatttccagagacaactccaagaaaatgttgtacctgcaaatgaacaaccagcgacctgatgacacggctgtttattattgt gcgagagggactatgcttcggggagtcaccaaagcgagatacggtctggacgtc ARGTMLRGVTKARYGLDV IGHV3-30 IGHD3-10 IGHJ6 ggattcaccttcagtacctatgct atgtactggatccgccaggctccaggcaaggggccagagtgggtgtcagtg atatcacatgatggaaataaggaa gaatacgcagactccgtgaagggccgattcaccatttccagagacaactccaagaaaatgttgtacctgcaaatgaacaaccagcgacctgatgacacggctgtttattattgt gcgagagggactatgcttcggggagtcaccaaagcgagatacggtctggacgtc IGG1 25 213 13 12 8 15 5 10 8 0 12 5 JY8QFUQ01AGSEB 16895 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 950 93.12 203/218 nt NA Homsap IGHJ3*02 F 250 100 50/50 nt Homsap IGHD5-12*01 F 1 8 8 14 8.8.14 [1.17.38.11] CVKRLEGVADDAFDIW in-frame - cctctggattcacctttagcagctatgccatgggctgggtccgccaggctccagggaaggggctggaatgggtctcaactattagtgggagtggtcggagcacatactacgcagactccgtgaagggccggtacaccatctccagagacaattccaagaatacgatgtctgtgcaaatgagcagcctgagagtcgaggacacggccatatattattgtgtgaaaaggttggaaggagtggctgatgatgcttttgatatctggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [291] a118>g,S40>G(+ + -)|g153>a|g163>a,A55>T(- - -)|t174>g|g187>c,G63>R(- - -)|t189>g,G63>R(- - -)|t227>a,F76>Y(- + -)|c255>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|c265>g,L89>V(+ - +)|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|c309>t|c314>t,A105>V(+ - +)| a118>g,S40>G(+ + -)|g153>a|g163>a,A55>T(- - -)| t174>g|g187>c,G63>R(- - -)|t189>g,G63>R(- - -)| t227>a,F76>Y(- + -)|c255>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|c265>g,L89>V(+ - +)|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|c309>t| c314>t,A105>V(+ - +)|g319>a|a320>g| 318 (320) 224 (226) 208 16 (18) 4 (6) 12 2 (3) 3 (4) 4 0 1 1 0 1 1 1 0 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 48 3 1 2 1 2 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 0 0 0 0 0 0 0 0 1 0 0 2 117 114 105 9 2 7 1 1 3 0 1 1 0 1 0 1 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 (1) 0 (1) 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagcagctatgcc atgggctgggtccgccaggctccagggaaggggctggaatgggtctcaact attagtgggagtggtcggagcaca tactacgcagactccgtgaagggccggtacaccatctccagagacaattccaagaatacgatgtctgtgcaaatgagcagcctgagagtcgaggacacggccatatattattgt gtgaaaaggttggaaggagtggctgatgatgcttttgatatc VKRLEGVADDAFDI IGHV3-23 IGHD5-12 IGHJ3 ggattcacctttagcagctatgcc atgggctgggtccgccaggctccagggaaggggctggaatgggtctcaact attagtgggagtggtcggagcaca tactacgcagactccgtgaagggccggtacaccatctccagagacaattccaagaatacgatgtctgtgcaaatgagcagcctgagagtcgaggacacggccatatattattgt gtgaaaaggttggaaggagtggctgatgatgcttttgatatc IGA2 15 213 8 7 6 9 2 6 3 1 9 2 JY8QFUQ01AGTKI 12573 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 892 89.95 197/219 nt NA Homsap IGHJ3*01 F 178 84 42/50 nt Homsap IGHD6-19*01 F 1 8 8 16 8.8.16 [2.17.38.11] CAKEQRGSGWSRSALDVW in-frame - gcctctggattcacgtttggcagccacgccatgagctgggtccgccaggctccagggaaggggctggagtacgtctcaattgttactggtagcggacgcagcacatactacgcagagtctgtgaagggccggttcaccgtctccagagacaattccaaggacaccctgtatctgcaaatggacagcctgagagccgaggacacggccgtgtattattgtgcgaaagaacaacgaggcagtggctggtcccgatcggctcttgatgtctggggccaaggggcatgggtcaccgtctcttcagcctccaccaagggcccatcgtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG3 93 88 [298, 299] c87>g|a103>g,S35>G(+ + -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|g163>a,A55>I(+ - +)|c164>t,A55>I(+ - +)|a166>g,I56>V(+ - +)|g170>c,S57>T(+ - +)|t177>c|t186>a|g187>c,G63>R(- - -)|t189>c,G63>R(- - -)|c207>g,D69>E(+ - +)|c210>t|a232>g,I78>V(+ - +)|a253>g,N85>D(+ + -)|g258>c|a274>g,N92>D(+ + -)|a303>g|c309>t| c87>g|a103>g,S35>G(+ + -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)| g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|g163>a,A55>I(+ - +)|c164>t,A55>I(+ - +)| a166>g,I56>V(+ - +)|g170>c,S57>T(+ - +)|t177>c|t186>a|g187>c,G63>R(- - -)|t189>c,G63>R(- - -)| c207>g,D69>E(+ - +)|c210>t|a232>g,I78>V(+ - +)|a253>g,N85>D(+ + -)|g258>c|a274>g,N92>D(+ + -)|a303>g|c309>t| 320 227 205 22 7 15 6 2 3 4 0 0 0 1 4 2 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 0 2 0 0 0 0 0 1 0 0 51 51 47 4 0 4 0 2 1 0 0 0 0 0 1 0 0 0 30 24 18 6 2 4 1 0 0 2 0 0 0 1 2 0 0 0 117 114 106 8 4 4 4 0 2 0 0 0 0 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacgtttggcagccacgcc atgagctgggtccgccaggctccagggaaggggctggagtacgtctcaatt gttactggtagcggacgcagcaca tactacgcagagtctgtgaagggccggttcaccgtctccagagacaattccaaggacaccctgtatctgcaaatggacagcctgagagccgaggacacggccgtgtattattgt gcgaaagaacaacgaggcagtggctggtcccgatcggctcttgatgtc AKEQRGSGWSRSALDV IGHV3-23 IGHD6-19 IGHJ3 ggattcacgtttggcagccacgcc atgagctgggtccgccaggctccagggaaggggctggagtacgtctcaatt gttactggtagcggacgcagcaca tactacgcagagtctgtgaagggccggttcaccgtctccagagacaattccaaggacaccctgtatctgcaaatggacagcctgagagccgaggacacggccgtgtattattgt gcgaaagaacaacgaggcagtggctggtcccgatcggctcttgatgtc IGG3 22 213 15 7 5 11 10 11 4 3 8 7 JY8QFUQ01AGWXH 4644 productive Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 873 89.4 194/217 nt NA Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD3-10*01 F 1 8 8 11 8.8.11 [1.17.38.11] CARVGTFNWFGPW in-frame + ctctggattcagttttgcagattatggcatgggctgggtccgccaacttccagggaaggggctggaatgggtcggtggtgttaattggaatgggggcagcgcaggttatgcagtctctgtggagggccgattcatcatctccagagacaacggcaagaagtccctgtatttgcaaatgaacagtctgagagtcgaggacacggccgtgtattactgtgcgagagtcggcacgttcaactggttcggcccctggggccagggaacgctggtcaccgtctcctctgcatccccggaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcc NA IGA1 100 95 [282] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a104>c,D35>A(- - -)|t105>a,D35>A(- - -)|a118>g,S40>G(+ + -)|g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|g153>a|t160>g,S54>G(+ + -)|c161>g,S54>G(+ + -)|a166>g,I56>V(+ - +)|t186>g|t189>c|a193>g,T65>A(- - -)|a206>t,D69>V(- - -)|a214>g,K72>E(+ - -)|c230>t,T77>I(- - -)|c248>g,A83>G(- + -)|c255>g,N85>K(+ - -)|c265>t|c287>t,A96>V(+ - +)|t301>g,L101>V(+ - +)|c307>t,H103>Y(+ - -)| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a104>c,D35>A(- - -)|t105>a,D35>A(- - -)| a118>g,S40>G(+ + -)|g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|g153>a|t160>g,S54>G(+ + -)|c161>g,S54>G(+ + -)| a166>g,I56>V(+ - +)|t186>g|t189>c|a193>g,T65>A(- - -)| a206>t,D69>V(- - -)|a214>g,K72>E(+ - -)|c230>t,T77>I(- - -)|c248>g,A83>G(- + -)|c255>g,N85>K(+ - -)|c265>t|c287>t,A96>V(+ - +)|t301>g,L101>V(+ - +)|c307>t,H103>Y(+ - -)| a320>t| 319 (320) 224 (225) 201 23 (24) 4 (5) 19 4 1 6 1 1 0 1 (2) 1 1 4 0 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 1 0 1 0 0 1 0 1 0 0 51 51 45 6 1 5 1 1 1 0 0 0 0 0 1 1 0 1 30 24 20 4 2 2 2 0 0 1 0 0 0 0 0 0 0 1 117 114 105 9 1 8 1 0 4 0 0 0 1 0 0 2 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ctct ggattcagttttgcagattatggc atgggctgggtccgccaacttccagggaaggggctggaatgggtcggtggt gttaattggaatgggggcagcgca ggttatgcagtctctgtggagggccgattcatcatctccagagacaacggcaagaagtccctgtatttgcaaatgaacagtctgagagtcgaggacacggccgtgtattactgt gcgagagtcggcacgttcaactggttcggcccc ARVGTFNWFGP IGHV3-20 IGHD3-10 IGHJ5 ggattcagttttgcagattatggc atgggctgggtccgccaacttccagggaaggggctggaatgggtcggtggt gttaattggaatgggggcagcgca ggttatgcagtctctgtggagggccgattcatcatctccagagacaacggcaagaagtccctgtatttgcaaatgaacagtctgagagtcgaggacacggccgtgtattactgt gcgagagtcggcacgttcaactggttcggcccc IGA1 23 213 12 11 7 12 5 11 2 2 13 6 JY8QFUQ01AGXB8 17233 productive Homsap IGHV3-21*04 F 963 94.01 204/217 nt NA Homsap IGHJ6*02 F, or Homsap IGHJ6*03 F (see comment) 163 76.79 43/56 nt Homsap IGHD6-13*01 F 3 8 8 10 8.8.10 [1.17.38.11] CARAVSHQVDVW in-frame + Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) ctctggattcgacttcagtagatatatcatgaattgggtccgccaggctccagggaaggggctggagtgggtctcatccattagttttgatagtaattacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcgctgtttctgcaaatgaacagcctgagagccgatgacacggccgtgtattactgtgcgagagccgtgtcgcatcaggtggacgtctggggccaaggggccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [278] a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|c108>a,S36>R(- - -)|g113>t,S38>I(- - -)|c120>t|a172>t,S58>F(- - -)|g173>t,S58>F(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g188>a,S63>N(- - -)|a258>g|a263>t,Y88>F(- + -)|g291>t,E97>D(+ - +)| a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|c108>a,S36>R(- - -)|g113>t,S38>I(- - -)| c120>t| a172>t,S58>F(- - -)|g173>t,S58>F(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g188>a,S63>N(- - -)| a258>g|a263>t,Y88>F(- + -)|g291>t,E97>D(+ - +)| a320>c| 319 (320) 224 (225) 211 13 (14) 2 (3) 11 3 2 1 0 0 (1) 2 2 0 0 0 3 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 0 0 0 0 2 0 0 0 0 1 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 19 5 0 5 1 2 0 0 0 0 1 0 0 0 1 0 117 114 111 3 1 2 1 0 0 0 0 0 1 0 0 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcgacttcagtagatatatc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagttttgatagtaattacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcgctgtttctgcaaatgaacagcctgagagccgatgacacggccgtgtattactgt gcgagagccgtgtcgcatcaggtggacgtc ARAVSHQVDV IGHV3-21 IGHD6-13 IGHJ6 ggattcgacttcagtagatatatc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagttttgatagtaattacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcgctgtttctgcaaatgaacagcctgagagccgatgacacggccgtgtattactgt gcgagagccgtgtcgcatcaggtggacgtc IGA2 13 213 6 7 3 8 3 5 2 0 2 9 JY8QFUQ01AGXOC 15208 productive Homsap IGHV3-66*02 F 840 88.32 189/214 nt NA Homsap IGHJ6*02 F (see comment) 197 80.33 49/61 nt Homsap IGHD2-8*01 F 3 8 7 11 8.7.11 [1.17.38.11] CASHLMVYALDVW in-frame - Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ctctggattcaccgtcagtggcaagtacatgagctgggtccgccaggctccagggcagggactggagtgggtctcagttatctatagtactggtagtacatactacgcagattccgtgaaagggcggttcaccatctccagagacagttccaacaacactctatatcttcaaatttacggcctgagagctgacgacacggctacttactactgtgcgagtcacttaatggtgtatgctctggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [278] a106>g,S36>G(+ + -)|c111>g,N37>K(+ - -)|a142>c,K48>Q(+ - -)|g147>a|t168>c|c174>t|g175>a,G59>T(+ - -)|g176>c,G59>T(+ - -)|c192>t|c207>t|g216>a|c222>g|a225>g|a245>g,N82>S(- - -)|g252>c,K84>N(+ - -)|g258>t|g261>a|g273>t,M91>I(+ + -)|a274>t,N92>Y(- - -)|a277>g,S93>G(+ + -)|g291>c,E97>D(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|t306>c| a106>g,S36>G(+ + -)|c111>g,N37>K(+ - -)| a142>c,K48>Q(+ - -)|g147>a| t168>c|c174>t|g175>a,G59>T(+ - -)|g176>c,G59>T(+ - -)|c192>t| c207>t|g216>a|c222>g|a225>g|a245>g,N82>S(- - -)|g252>c,K84>N(+ - -)|g258>t|g261>a|g273>t,M91>I(+ + -)|a274>t,N92>Y(- - -)|a277>g,S93>G(+ + -)|g291>c,E97>D(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|t306>c| a318>t,R106>S(- - -)| 317 (318) 219 (220) 194 25 (26) 11 14 (15) 4 5 3 3 1 0 1 (2) 0 3 2 3 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 1 0 0 51 51 49 2 1 1 0 1 0 0 1 0 0 0 0 0 0 0 30 21 16 5 3 2 0 1 2 1 0 0 0 0 1 0 0 0 117 114 98 16 7 9 3 3 1 2 0 0 1 0 2 1 3 0 5 (6) 5 5 0 (1) 0 0 (1) 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccgtcagtggcaagtac atgagctgggtccgccaggctccagggcagggactggagtgggtctcagtt atctatagtactggtagtaca tactacgcagattccgtgaaagggcggttcaccatctccagagacagttccaacaacactctatatcttcaaatttacggcctgagagctgacgacacggctacttactactgt gcgagtcacttaatggtgtatgctctggacgtc ASHLMVYALDV IGHV3-66 IGHD2-8 IGHJ6 ggattcaccgtcagtggcaagtac atgagctgggtccgccaggctccagggcagggactggagtgggtctcagtt atctatagtactggtagtaca tactacgcagattccgtgaaagggcggttcaccatctccagagacagttccaacaacactctatatcttcaaatttacggcctgagagctgacgacacggctacttactactgt gcgagtcacttaatggtgtatgctctggacgtc IGA1 25 210 15 10 8 16 7 9 8 3 10 4 JY8QFUQ01AGZC5 21316 productive Homsap IGHV3-48*03 F 873 89.4 194/217 nt NA Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD3-3*01 F 2 8 8 15 8.8.15 [1.17.38.11] CARESWWSGFYGWYDPW in-frame + ctctggattcacctttagtagttctgccatgaactgggtccgccaggctccagggaagggactggagtggatttcatacattagtgatagtggtagtcgcatttactatgcagactctgtgaggggccgattcaccatctccagagacgacgccaagaacgtgctgtatctgcaaatgaacggactgcgagacgaagacacggctctttattactgtgcgagagaatcctggtggagtggcttttatgggtggtacgacccctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 100 100 [294] c90>t|a110>c,Y37>S(+ - -)|a113>c,E38>A(- - -)|a114>c,E38>A(- - -)|g147>a|g157>a,V53>I(+ - +)|a172>g,S58>D(- - -)|g173>a,S58>D(- - -)|a190>c,T64>R(- - -)|c191>g,T64>R(- - -)|a195>t|c201>t|a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|t256>g,S86>V(- - -)|c257>t,S86>V(- - -)|a258>g,S86>V(- - -)|a277>g,S93>G(+ + -)|c279>a,S93>G(+ + -)|a283>c|c287>a,A96>D(- - -)|g291>a|g301>c,V101>L(+ - +)| c90>t|a110>c,Y37>S(+ - -)|a113>c,E38>A(- - -)|a114>c,E38>A(- - -)| g147>a|g157>a,V53>I(+ - +)| a172>g,S58>D(- - -)|g173>a,S58>D(- - -)|a190>c,T64>R(- - -)|c191>g,T64>R(- - -)|a195>t| c201>t|a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|t256>g,S86>V(- - -)|c257>t,S86>V(- - -)|a258>g,S86>V(- - -)|a277>g,S93>G(+ + -)|c279>a,S93>G(+ + -)|a283>c|c287>a,A96>D(- - -)|g291>a|g301>c,V101>L(+ - +)| 320 225 202 23 6 17 5 4 3 0 5 2 1 0 1 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 1 0 3 0 0 0 0 0 0 0 51 51 49 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 30 24 19 5 1 4 1 1 0 0 1 0 1 0 0 1 0 0 117 114 102 12 3 9 4 1 2 0 1 2 0 0 1 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,113-114(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtagttctgcc atgaactgggtccgccaggctccagggaagggactggagtggatttcatac attagtgatagtggtagtcgcatt tactatgcagactctgtgaggggccgattcaccatctccagagacgacgccaagaacgtgctgtatctgcaaatgaacggactgcgagacgaagacacggctctttattactgt gcgagagaatcctggtggagtggcttttatgggtggtacgacccc ARESWWSGFYGWYDP IGHV3-48 IGHD3-3 IGHJ5 ggattcacctttagtagttctgcc atgaactgggtccgccaggctccagggaagggactggagtggatttcatac attagtgatagtggtagtcgcatt tactatgcagactctgtgaggggccgattcaccatctccagagacgacgccaagaacgtgctgtatctgcaaatgaacggactgcgagacgaagacacggctctttattactgt gcgagagaatcctggtggagtggcttttatgggtggtacgacccc IGG1 23 213 12 11 7 11 5 12 4 2 10 7 JY8QFUQ01AH0X0 2283 productive (see comment) Homsap IGHV4-61*01 F, or Homsap IGHV4-61*08 F 920 90.95 201/221 nt 90.5 200/221 nt Homsap IGHJ5*02 F (see comment) 147 76.47 39/51 nt Homsap IGHD1-26*01 F 3 10 7 15 10.7.15 [1.17.38.11] CARGGSGGSYWNLIRDW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in CDR1-IMGT at codon 36 of V-REGION: 'C' (1 nucleotides at position 29 to 29 in the user submitted sequence), (cause frameshift); tctctgatgactccgtcagcagtggtcgCttactactggagttgggtccggcagcccccagggaagggactggagtggattggtcatttctatcacattgggggcactaagtacaacccctccctcgcgagtcgagtcaccatatcagtagacacgtccaagagccagttctccctgatgctgaactctgtgaccgctgcggacacggccgtatatttctgtgcgagaggtgggtccggtgggagctactggaatctgatccgggactggggccagggaaccctggtcaccgtctactcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [298] g80>a,G27>D(- - -)|g83>a,G28>D(- - -)|a106>c,S36>R(- - -)|c120>t|a124>g,I42>V(+ - +)|g162>t|t163>c,Y55>H(+ - -)|a166>t,I56>F(+ - -)|t172>c,Y58>H(+ - -)|g176>t,S59>I(- - -)|a190>g,S64>G(+ + -)|c195>t|c198>g,N66>K(+ - -)|a214>g,K72>A(- - -)|a215>c,K72>A(- - -)|a254>g,N85>S(- - -)|a269>t,K90>M(- + -)|g275>a,S92>N(- - -)|g303>a|a308>t,Y103>F(- + -)| g80>a,G27>D(- - -)|g83>a,G28>D(- - -)|a106>c,S36>R(- - -)| c120>t|a124>g,I42>V(+ - +)|g162>t|t163>c,Y55>H(+ - -)| a166>t,I56>F(+ - -)|t172>c,Y58>H(+ - -)|g176>t,S59>I(- - -)|a190>g,S64>G(+ + -)|c195>t| c198>g,N66>K(+ - -)|a214>g,K72>A(- - -)|a215>c,K72>A(- - -)|a254>g,N85>S(- - -)|a269>t,K90>M(- + -)|g275>a,S92>N(- - -)|g303>a|a308>t,Y103>F(- + -)| a320>g| 319 (320) 228 (229) 208 20 (21) 4 (5) 16 4 (5) 4 2 2 2 0 3 0 0 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 0 2 0 0 1 0 0 0 0 0 0 0 51 51 47 4 2 2 1 0 1 1 0 0 0 0 0 0 1 0 30 21 16 5 1 4 1 0 1 1 0 0 1 0 0 0 1 0 117 114 106 8 1 7 2 2 0 0 1 0 2 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct gatgactccgtcagcagtggtcgttactac tggagttgggtccggcagcccccagggaagggactggagtggattggtcat ttctatcacattgggggcact aagtacaacccctccctcgcgagtcgagtcaccatatcagtagacacgtccaagagccagttctccctgatgctgaactctgtgaccgctgcggacacggccgtatatttctgt gcgagaggtgggtccggtgggagctactggaatctgatccgggac ARGGSGGSYWNLIRD IGHV4-61 IGHD1-26 IGHJ5 gatgactccgtcagcagtggtcgttactac tggagttgggtccggcagcccccagggaagggactggagtggattggtcat ttctatcacattgggggcact aagtacaacccctccctcgcgagtcgagtcaccatatcagtagacacgtccaagagccagttctccctgatgctgaactctgtgaccgctgcggacacggccgtatatttctgt gcgagaggtgggtccggtgggagctactggaatctgatccgggac IGA1 20 216 12 8 6 9 6 11 3 1 9 7 JY8QFUQ01AH32A 20971 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 842 87.61 191/218 nt NA Homsap IGHJ6*02 F 247 88.71 55/62 nt Homsap IGHD2-8*01 F 1 8 8 19 8.8.19 [1.17.38.11] CAKVFQVSGEHHFYYYGMNAW in-frame + cctctggattcatctttagtaattatgccatgagttgggtccgccaggccccagggagggggctggagtgggtctcaactatcagtgccaatggagacaacacatactacgcggactccgtgaagggccgattcaccatctccagagacaattccaagagcacagtgtatatgcaaatgaacagcctgaaagccgaggagacggccgtctatcattgtgcgaaagtcttccaggtgtctggcgaacatcacttctactactatggtatgaatgcctggggccaagggaccacggtcaccgtctccccagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [306] c86>t,T29>I(- - -)|c105>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|c120>t|t135>c|a143>g,K48>R(+ + +)|g163>a,A55>T(- - -)|t168>c|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g176>a,S59>N(- - -)|t186>a|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|g191>a,S64>N(- - -)|a204>g|g225>a|a254>g,N85>S(- - -)|g258>a|c259>g,L87>V(+ - +)|c265>a,L89>M(+ + -)|g284>a,R95>K(+ + +)|c294>g,D98>E(+ - +)|a303>c|t307>c,Y103>H(+ - -)|c309>t,Y103>H(+ - -)| c86>t,T29>I(- - -)|c105>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| c120>t|t135>c|a143>g,K48>R(+ + +)|g163>a,A55>T(- - -)| t168>c|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g176>a,S59>N(- - -)|t186>a|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|g191>a,S64>N(- - -)| a204>g|g225>a|a254>g,N85>S(- - -)|g258>a|c259>g,L87>V(+ - +)|c265>a,L89>M(+ + -)|g284>a,R95>K(+ + +)|c294>g,D98>E(+ - +)|a303>c|t307>c,Y103>H(+ - -)|c309>t,Y103>H(+ - -)| a320>t| 319 (320) 225 (226) 198 27 (28) 9 (10) 18 3 8 5 5 1 1 0 (1) 1 1 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 3 0 0 0 0 0 0 0 0 0 51 51 47 4 2 2 1 1 1 1 0 0 0 0 0 0 0 0 30 24 16 8 2 6 0 3 0 3 0 0 0 1 1 0 0 0 117 114 103 11 4 7 2 3 1 1 1 1 0 0 0 2 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcatctttagtaattatgcc atgagttgggtccgccaggccccagggagggggctggagtgggtctcaact atcagtgccaatggagacaacaca tactacgcggactccgtgaagggccgattcaccatctccagagacaattccaagagcacagtgtatatgcaaatgaacagcctgaaagccgaggagacggccgtctatcattgt gcgaaagtcttccaggtgtctggcgaacatcacttctactactatggtatgaatgcc AKVFQVSGEHHFYYYGMNA IGHV3-23 IGHD2-8 IGHJ6 ggattcatctttagtaattatgcc atgagttgggtccgccaggccccagggagggggctggagtgggtctcaact atcagtgccaatggagacaacaca tactacgcggactccgtgaagggccgattcaccatctccagagacaattccaagagcacagtgtatatgcaaatgaacagcctgaaagccgaggagacggccgtctatcattgt gcgaaagtcttccaggtgtctggcgaacatcacttctactactatggtatgaatgcc IGA1 27 213 21 6 13 17 8 10 6 3 9 9 JY8QFUQ01AH38B 14566 productive (see comment) Homsap IGHV4-61*02 F 806 85.78 187/218 nt 85.32 186/218 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-16*01 F 3 10 6 16 10.6.16 [1.17.38.11] CAIERSRSIGGDGQFDFW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 64 of V-REGION: 3 nucleotides (from position 102 in the user submitted sequence), (do not cause frameshift) tttctggtggccccatcagaggcggtggtttctactggacctggctccggcagtccgccgggaagggcctggagtggatcggacgtatttatgacagtggcagtgactacaatccttctctcaagagtcgagtcaccatgtcagtagacacgtccaagagccagttctccctgaggctgagttctgtggccgccgcagacacggccgtttacttctgtgcgatagaacgtagtagatcgattggcggagacggacaatttgacttctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 100 [297] c75>t|t85>c,S29>P(+ - -)|c93>a,S31>R(- - -)|a100>g,S34>G(+ + -)|t102>c,S34>G(+ + -)|a106>g,S36>G(+ + -)|a110>t,Y37>F(- + -)|g119>c,S40>T(+ - +)|a124>c,I42>L(+ + +)|c133>t,P45>S(+ - -)|a147>c|t159>c|g162>a|c168>t|a172>g,T58>D(- + -)|c173>a,T58>D(- + -)|g189>c|c194>g,T65>S(+ - +)|c195>t,T65>S(+ - +)|a196>g,N66>D(+ + -)|c204>t|c207>t|c210>t|a234>g,I78>M(+ + -)|a254>g,N85>S(- - -)|a269>g,K90>R(+ + +)|c276>t|a283>g,T95>A(- - -)|g303>t|t306>c|a308>t,Y103>F(- + -)|g317>t,R106>I(- + -)| c75>t| t85>c,S29>P(+ - -)|c93>a,S31>R(- - -)|a100>g,S34>G(+ + -)|t102>c,S34>G(+ + -)|a106>g,S36>G(+ + -)|a110>t,Y37>F(- + -)| g119>c,S40>T(+ - +)|a124>c,I42>L(+ + +)|c133>t,P45>S(+ - -)|a147>c|t159>c|g162>a| c168>t|a172>g,T58>D(- + -)|c173>a,T58>D(- + -)|g189>c|c194>g,T65>S(+ - +)|c195>t,T65>S(+ - +)| a196>g,N66>D(+ + -)|c204>t|c207>t|c210>t|a234>g,I78>M(+ + -)|a254>g,N85>S(- - -)|a269>g,K90>R(+ + +)|c276>t|a283>g,T95>A(- - -)|g303>t|t306>c|a308>t,Y103>F(- + -)| g317>t,R106>I(- + -)| 320 226 194 32 12 20 8 1 8 4 2 2 2 0 2 1 2 0 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 30 24 6 0 6 2 0 0 2 0 1 1 0 0 0 0 0 51 51 45 6 3 3 0 1 1 1 2 0 0 0 1 0 0 0 30 18 12 6 2 4 1 0 2 0 0 1 0 0 1 1 0 0 117 114 102 12 6 6 5 0 4 1 0 0 1 0 0 0 1 0 8 8 7 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tttct ggtggccccatcagaggcggtggtttctac tggacctggctccggcagtccgccgggaagggcctggagtggatcggacgt atttatgacagtggcagt gactacaatccttctctcaagagtcgagtcaccatgtcagtagacacgtccaagagccagttctccctgaggctgagttctgtggccgccgcagacacggccgtttacttctgt gcgatagaacgtagtagatcgattggcggagacggacaatttgacttc AIERSRSIGGDGQFDF IGHV4-61 IGHD3-16 IGHJ4 ggtggccccatcagaggcggtggtttctac tggacctggctccggcagtccgccgggaagggcctggagtggatcggacgt atttatgacagtggcagt gactacaatccttctctcaagagtcgagtcaccatgtcagtagacacgtccaagagccagttctccctgaggctgagttctgtggccgccgcagacacggccgtttacttctgt gcgatagaacgtagtagatcgattggcggagacggacaatttgacttc IGA1 30 213 20 10 8 14 12 16 9 2 9 10 JY8QFUQ01AH5A1 19106 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 969 93.64 206/220 nt 92.73 204/220 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-19*01 F 1 10 7 13 10.7.13 [1.16.38.11] CARHGSGWLQELDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 13 to 13 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 45 of V-REGION: 1 nucleotides (from position 56 in the user submitted sequence), (cause frameshift) tctctggtggctGccatcatcagagacagtgcctactggggctggatccgccagccccagggaaggggctggagtggcttgggagcatctattatagtgggagtacctactacaatccctccctcaagagtcgagtcaccatatccgtagacacgtccaagaagcagttctccctgaagctgagctctgtgaccgccgcagacacggctgtatattactgtgcgagacatggcagtggctggctccaggaacttgactactggggccagggaaccctggtcaccgtctccacagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [291] g92>t,S31>I(- - -)|t102>a,S34>R(- - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|t105>c,S35>D(- - -)|t109>g,Y37>A(- - -)|a110>c,Y37>A(- - -)|a157>c,I53>L(+ + +)|t165>c|c192>t|c204>t|g207>c|c255>g,N85>K(+ - -)|g303>a| g92>t,S31>I(- - -)|t102>a,S34>R(- - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|t105>c,S35>D(- - -)|t109>g,Y37>A(- - -)|a110>c,Y37>A(- - -)| a157>c,I53>L(+ + +)|t165>c| c192>t| c204>t|g207>c|c255>g,N85>K(+ - -)|g303>a| 320 228 214 14 5 9 1 2 2 2 2 0 0 1 1 1 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 23 7 0 7 1 1 0 1 1 0 0 1 0 0 1 1 51 50 48 2 1 1 0 0 0 1 1 0 0 0 0 0 0 0 30 21 20 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 117 114 110 4 3 1 0 1 1 0 0 0 0 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcatcagagacagtgcctac tggggctggatccgccagccccagggaaggggctggagtggcttgggagc atctattatagtgggagtacc tactacaatccctccctcaagagtcgagtcaccatatccgtagacacgtccaagaagcagttctccctgaagctgagctctgtgaccgccgcagacacggctgtatattactgt gcgagacatggcagtggctggctccaggaacttgactac ARHGSGWLQELDY IGHV4-39 IGHD6-19 IGHJ4 ggtggctccatcatcagagacagtgcctac tggggctggatccgccagccccagggaaggggctggagtggcttgggagc atctattatagtgggagtacc tactacaatccctccctcaagagtcgagtcaccatatccgtagacacgtccaagaagcagttctccctgaagctgagctctgtgaccgccgcagacacggctgtatattactgt gcgagacatggcagtggctggctccaggaacttgactac IGA1 14 215 7 7 4 7 3 7 4 1 2 7 JY8QFUQ01AH5X7 19068 productive Homsap IGHV6-1*01 F, or Homsap IGHV6-1*02 F 1085 97.8 222/227 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD1-26*01 F 3 10 9 19 10.9.19 [1.17.38.11] CTRGSSRGGRYDENYYELDFW in-frame - tctccggggacagtgtctctaccaacagagctgcttggaactggatcaggcagtccccatcgagaggccttgagtggctgggaaggacatactacaggtccaagtggtataatgattatgcagtgtctgtgaaaagtcgaataaccatcaacccagacacatccaagaaccagttctccctgcagttgaattctgtgactcccgaggacacggctgtgtattactgtacaagagggtccagccgcggggggagatatgatgagaactactatgagctggacttctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [315] g101>c,S34>T(+ - +)|t108>a,S36>R(- - -)|a207>g|c271>t|c276>t|g313>a,A105>T(- - -)| g101>c,S34>T(+ - +)|t108>a,S36>R(- - -)| a207>g|c271>t|c276>t| g313>a,A105>T(- - -)|a320>g| 319 (320) 234 (235) 228 6 (7) 3 (4) 3 1 (2) 1 2 0 0 0 0 1 1 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 0 2 0 0 0 0 0 0 0 1 1 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 27 27 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 111 3 3 0 1 0 2 0 0 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 1 0 0 0 0 0 0 0 0 0 0 ta,5-6(FR1)|aa,40-41(FR1)|aa,103-104(CDR1)|aa,118-119(FR2)|aa,162-163(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,184-185(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|ta,206-207(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,227-228(FR3)|aa,235-236(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,5-6(FR1)|tt,114-115(CDR1)|tt,149-150(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|tt,198-199(FR3)|ta,206-207(FR3)|ta,227-228(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggta,3-6(FR1)|agct,8-11(FR1)|agca,14-17(FR1)|agca,100-103(CDR1)|ggca,128-131(FR2)|ggct,155-158(FR2)|ggta,188-191(CDR2)|agta,204-207(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tgcc,69-72(FR1)|tgct,108-111(CDR1)|aact,118-121(FR2)|tact,169-172(CDR2)|aacc,228-231(FR3)|aacc,235-238(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|aact,274-277(FR3)|tact,307-310(FR3)| tctcc ggggacagtgtctctaccaacagagctgct tggaactggatcaggcagtccccatcgagaggccttgagtggctgggaagg acatactacaggtccaagtggtataat gattatgcagtgtctgtgaaaagtcgaataaccatcaacccagacacatccaagaaccagttctccctgcagttgaattctgtgactcccgaggacacggctgtgtattactgt acaagagggtccagccgcggggggagatatgatgagaactactatgagctggacttc TRGSSRGGRYDENYYELDF IGHV6-1 IGHD1-26 IGHJ4 ggggacagtgtctctaccaacagagctgct tggaactggatcaggcagtccccatcgagaggccttgagtggctgggaagg acatactacaggtccaagtggtataat gattatgcagtgtctgtgaaaagtcgaataaccatcaacccagacacatccaagaaccagttctccctgcagttgaattctgtgactcccgaggacacggctgtgtattactgt acaagagggtccagccgcggggggagatatgatgagaactactatgagctggacttc IGA1 5 222 3 2 2 3 1 2 3 0 0 2 JY8QFUQ01AH715 8947 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 868 89.35 193/216 nt 88.89 192/216 nt Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F (see comment) 186 87.5 42/48 nt Homsap IGHD6-6*01 F 2 7 8 15 7.8.15 [1.17.38.11] CAKGDSPIASRLYIDSW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) in CDR1-IMGT, from codon 29 of V-REGION: 1 nucleotides (from position 12 in the user submitted sequence), (cause frameshift) ctctggattcattttagcaattatgccatgaactgggtccgccaggctccagggaaggggccggagtgggtctcagcttttagtggtggtggcactaagacctactacgcagactccgtgaagggccggttcttcatctccagagacaattccaagaacactctacatctgcatatgagcagcctgagggccgaggacacggccacatattactgtgcgaaaggggactcgcctatagcgtctcgtctatatattgactcctggggccaggggaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [293] c87>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g119>a,S40>N(- - -)|t149>c,L50>P(- - -)|a166>t,I56>F(+ - -)|a175>g,S59>G(+ + -)|t186>c|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|g191>a,S64>K(- - -)|c192>g,S64>K(- - -)|a195>c|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|g258>t|g261>a|t262>c,Y88>H(+ - -)|a270>t,Q90>H(- + -)|a275>g,N92>S(- - -)|a285>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)| c87>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g119>a,S40>N(- - -)|t149>c,L50>P(- - -)| a166>t,I56>F(+ - -)|a175>g,S59>G(+ + -)|t186>c|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|g191>a,S64>K(- - -)|c192>g,S64>K(- - -)|a195>c| a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|g258>t|g261>a|t262>c,Y88>H(+ - -)|a270>t,Q90>H(- + -)|a275>g,N92>S(- - -)|a285>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)| a320>g| 319 (320) 223 (224) 200 23 (24) 6 (7) 17 3 (4) 6 3 4 1 0 3 0 1 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 20 3 1 2 0 1 2 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 30 24 16 8 2 6 1 2 0 1 1 0 1 0 1 1 0 0 117 114 104 10 3 7 2 2 1 2 0 0 2 0 0 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcattttagcaattatgcc atgaactgggtccgccaggctccagggaaggggccggagtgggtctcagct tttagtggtggtggcactaagacc tactacgcagactccgtgaagggccggttcttcatctccagagacaattccaagaacactctacatctgcatatgagcagcctgagggccgaggacacggccacatattactgt gcgaaaggggactcgcctatagcgtctcgtctatatattgactcc AKGDSPIASRLYIDS IGHV3-23 IGHD6-6 IGHJ4 ggattcattttagcaattatgcc atgaactgggtccgccaggctccagggaaggggccggagtgggtctcagct tttagtggtggtggcactaagacc tactacgcagactccgtgaagggccggttcttcatctccagagacaattccaagaacactctacatctgcatatgagcagcctgagggccgaggacacggccacatattactgt gcgaaaggggactcgcctatagcgtctcgtctatatattgactcc IGG2 23 212 16 7 9 12 7 11 3 3 9 8 JY8QFUQ01AHAA1 13938 productive Homsap IGHV3-48*01 F 927 92.17 200/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD5-12*01 F 1 8 8 15 8.8.15 [1.17.38.11] CAKVGSMNIEASPPDDW in-frame - ctctggattcagcttcagttcctatagcatgagctgggtccgccaggctccggggaaggggctggagtgggtctcatatattagtagtagtggtaggaccatctactacgctgactccgtgaagggccgattcaccatgtccagagacaatgccaagaattcactgtatctacaaatgaacagcctgagagccgaggacacggccgtctattactgtgcgaaagttggcagcatgaatatcgaggcgagccctcctgacgactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacatggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [293] c86>g,T29>S(+ - +)|a106>t|g107>c|a119>g,N40>S(- - -)|a138>g|t159>c|c165>t|a184>g,S62>G(+ + -)|t189>g,S63>R(- - -)|a195>c|a204>t|t210>c|c234>g,I78>M(+ + -)|c255>t|g267>a|t300>c|g303>c|g317>a,R106>K(+ + +)| c86>g,T29>S(+ - +)|a106>t|g107>c| a119>g,N40>S(- - -)|a138>g|t159>c|c165>t| a184>g,S62>G(+ + -)|t189>g,S63>R(- - -)|a195>c| a204>t|t210>c|c234>g,I78>M(+ + -)|c255>t|g267>a|t300>c|g303>c| g317>a,R106>K(+ + +)|a320>t| 319 (320) 224 (225) 206 18 (19) 12 (13) 6 3 2 2 3 1 0 2 (3) 0 2 2 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 0 0 0 0 1 0 1 1 0 0 51 51 47 4 3 1 2 0 1 1 0 0 0 0 0 0 0 0 30 24 21 3 1 2 1 0 0 0 1 0 0 0 0 0 0 1 117 114 107 7 6 1 0 1 1 2 0 0 1 0 1 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 1 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagcttcagttcctatagc atgagctgggtccgccaggctccggggaaggggctggagtgggtctcatat attagtagtagtggtaggaccatc tactacgctgactccgtgaagggccgattcaccatgtccagagacaatgccaagaattcactgtatctacaaatgaacagcctgagagccgaggacacggccgtctattactgt gcgaaagttggcagcatgaatatcgaggcgagccctcctgacgac AKVGSMNIEASPPDD IGHV3-48 IGHD5-12 IGHJ4 ggattcagcttcagttcctatagc atgagctgggtccgccaggctccggggaaggggctggagtgggtctcatat attagtagtagtggtaggaccatc tactacgctgactccgtgaagggccgattcaccatgtccagagacaatgccaagaattcactgtatctacaaatgaacagcctgagagccgaggacacggccgtctattactgt gcgaaagttggcagcatgaatatcgaggcgagccctcctgacgac IGA1 17 213 9 8 3 7 6 10 9 3 2 3 JY8QFUQ01AHB8A 6365 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 901 90.41 198/219 nt NA Homsap IGHJ4*02 F (see comment) 186 87.5 42/48 nt Homsap IGHD4-17*01 F 2 8 8 12 8.8.12 [2.17.38.11] CARADDYGDYPEFW in-frame - Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) gcctcaggattcaccttcagtatctacaccatgaagtgggtccgccaggctccagggaagggactggagtgggtctcatccatcactagccgtggtgcttacatacactacgcagcctcagtgaggggccgattcaccatctccagagacaacgccagggactcactgtatttgcaaatgaacagcctgagtgccgaggacacggctgtttattactgtgcgagagctgacgactacggtgactaccctgagttctggggccaggggaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgcttagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [286] t78>a|g107>t,S36>I(- - -)|t111>c|g113>c,S38>T(+ - +)|c120>g,N40>K(+ - -)|g147>a|t168>c|g170>c,S57>T(+ - +)|t174>c|a175>c,S59>R(- - -)|a184>g,S62>G(+ + -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t196>c,Y66>H(+ - -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a251>g,K84>R(+ + +)|a253>g,N85>D(+ + -)|c265>t|a285>t,R95>S(- - -)|g303>t| t78>a| g107>t,S36>I(- - -)|t111>c|g113>c,S38>T(+ - +)| c120>g,N40>K(+ - -)|g147>a| t168>c|g170>c,S57>T(+ - +)|t174>c|a175>c,S59>R(- - -)|a184>g,S62>G(+ + -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)| t196>c,Y66>H(+ - -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a251>g,K84>R(+ + +)|a253>g,N85>D(+ + -)|c265>t|a285>t,R95>S(- - -)|g303>t| a320>c| 319 (320) 226 (227) 205 21 (22) 7 (8) 14 5 1 1 4 2 (3) 0 1 1 3 1 2 0 78 6 5 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 21 3 1 2 0 0 0 1 0 0 0 0 1 0 1 0 51 51 49 2 1 1 0 1 0 0 0 0 0 0 0 1 0 0 30 24 17 7 2 5 2 0 0 2 1 0 0 0 2 0 0 0 117 114 106 8 2 6 3 0 1 1 1 0 1 0 0 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctca ggattcaccttcagtatctacacc atgaagtgggtccgccaggctccagggaagggactggagtgggtctcatcc atcactagccgtggtgcttacata cactacgcagcctcagtgaggggccgattcaccatctccagagacaacgccagggactcactgtatttgcaaatgaacagcctgagtgccgaggacacggctgtttattactgt gcgagagctgacgactacggtgactaccctgagttc ARADDYGDYPEF IGHV3-21 IGHD4-17 IGHJ4 ggattcaccttcagtatctacacc atgaagtgggtccgccaggctccagggaagggactggagtgggtctcatcc atcactagccgtggtgcttacata cactacgcagcctcagtgaggggccgattcaccatctccagagacaacgccagggactcactgtatttgcaaatgaacagcctgagtgccgaggacacggctgtttattactgt gcgagagctgacgactacggtgactaccctgagttc IGA1 20 213 11 9 2 8 9 12 3 3 7 7 JY8QFUQ01AHBK2 7914 productive Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F 858 89.25 191/214 nt NA Homsap IGHJ3*02 F (see comment) 196 88 44/50 nt Homsap IGHD6-13*01 F 1 8 7 14 8.7.14 [1.17.38.11] CARPTYSSGRDAFDTW in-frame + Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) ctatggtgggtccttcagtacttactactggacatggatccgccagcacccagagaagggactggagtggattggggaaatcaatcacagtggaagccccaactacagcccgtccctcaagagtcgagtcctcatatcgatagacacgtccaagaatcaggtctccctcaacctcttctctgtgaccgccgcggacacgggtgtgtattattgtgcgaggcccacatatagcagcggccgggacgcttttgatacctggggccaagggacgacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [287] g106>a,G36>T(+ - -)|g107>c,G36>T(+ - -)|g119>c,S40>T(+ - +)|c120>a,S40>T(+ - +)|c134>a,P45>H(+ - -)|g140>a,G47>E(- - -)|g147>a|t174>c|a193>c,T65>P(+ + -)|a203>g,N68>S(- - -)|a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|a237>g|g238>a,V80>I(+ - +)|c255>t|t259>g,F87>V(+ - -)|g267>c|g270>c,K90>N(+ - -)|g273>c|a274>t,S92>F(- - -)|g275>t,S92>F(- - -)|c299>g,A100>G(- + -)|c309>t| g106>a,G36>T(+ - -)|g107>c,G36>T(+ - -)| g119>c,S40>T(+ - +)|c120>a,S40>T(+ - +)|c134>a,P45>H(+ - -)|g140>a,G47>E(- - -)|g147>a| t174>c|a193>c,T65>P(+ + -)| a203>g,N68>S(- - -)|a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|a237>g|g238>a,V80>I(+ - +)|c255>t|t259>g,F87>V(+ - -)|g267>c|g270>c,K90>N(+ - -)|g273>c|a274>t,S92>F(- - -)|g275>t,S92>F(- - -)|c299>g,A100>G(- + -)|c309>t| a318>g|g319>c|g320>c| 317 (320) 219 (222) 196 23 (26) 7 (10) 16 2 (3) 4 3 1 2 2 1 0 5 (7) 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 51 51 46 5 1 4 0 2 0 0 0 2 0 0 1 0 0 0 30 21 19 2 1 1 0 0 0 1 1 0 0 0 0 0 0 0 117 114 100 14 5 9 2 1 3 0 1 0 1 0 3 1 1 1 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 0 0 0 0 0 0 0 (2) 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctat ggtgggtccttcagtacttactac tggacatggatccgccagcacccagagaagggactggagtggattggggaa atcaatcacagtggaagcccc aactacagcccgtccctcaagagtcgagtcctcatatcgatagacacgtccaagaatcaggtctccctcaacctcttctctgtgaccgccgcggacacgggtgtgtattattgt gcgaggcccacatatagcagcggccgggacgcttttgatacc ARPTYSSGRDAFDT IGHV4-34 IGHD6-13 IGHJ3 ggtgggtccttcagtacttactac tggacatggatccgccagcacccagagaagggactggagtggattggggaa atcaatcacagtggaagcccc aactacagcccgtccctcaagagtcgagtcctcatatcgatagacacgtccaagaatcaggtctccctcaacctcttctctgtgaccgccgcggacacgggtgtgtattattgt gcgaggcccacatatagcagcggccgggacgcttttgatacc IGA1 23 210 10 13 7 16 3 7 6 1 13 3 JY8QFUQ01AHEWI 14457 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F or Homsap IGHV3-15*04 F 998 94.2 211/224 nt NA Homsap IGHJ1*01 F (see comment) 161 78.85 41/52 nt Homsap IGHD1-26*01 F 3 8 10 9 8.10.9 [1.17.38.11] CTTGGGTNYYW in-frame - Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) cctctggattcactttcagtgacgcctggatgagctgggtccgccaggctccagggaaagggctggagtgggttggccgtattccaagcaaagctgatggtgggacaacagactacgctgcgcccgttaaaggcagattcaccatctcaagagaggattcgaaaaatatgctgtatctgcaattgaacagcctgaaaaccgaggacacagccgtgtatttctgtaccacagggggtggaactaactactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [283] a106>g,N36>D(+ + -)|g144>a|a169>c,K57>P(- - -)|a170>c,K57>P(- - -)|a178>g,T60>A(- - -)|a207>g|g213>t|t243>g,D81>E(+ - +)|a249>g|c255>t|c257>t,T86>M(- - -)|a271>t,M91>L(+ + -)|a308>t,Y103>F(- + -)| a106>g,N36>D(+ + -)| g144>a| a169>c,K57>P(- - -)|a170>c,K57>P(- - -)|a178>g,T60>A(- - -)| a207>g|g213>t|t243>g,D81>E(+ - +)|a249>g|c255>t|c257>t,T86>M(- - -)|a271>t,M91>L(+ + -)|a308>t,Y103>F(- + -)| a320>g| 319 (320) 231 (232) 218 13 (14) 5 (6) 8 4 (5) 1 2 0 2 0 2 0 0 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 30 27 3 0 3 1 0 0 0 2 0 0 0 0 0 0 0 117 114 106 8 4 4 2 0 2 0 0 0 2 0 0 0 1 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| cctct ggattcactttcagtgacgcctgg atgagctgggtccgccaggctccagggaaagggctggagtgggttggccgt attccaagcaaagctgatggtgggacaaca gactacgctgcgcccgttaaaggcagattcaccatctcaagagaggattcgaaaaatatgctgtatctgcaattgaacagcctgaaaaccgaggacacagccgtgtatttctgt accacagggggtggaactaactactac TTGGGTNYY IGHV3-15 IGHD1-26 IGHJ1 ggattcactttcagtgacgcctgg atgagctgggtccgccaggctccagggaaagggctggagtgggttggccgt attccaagcaaagctgatggtgggacaaca gactacgctgcgcccgttaaaggcagattcaccatctcaagagaggattcgaaaaatatgctgtatctgcaattgaacagcctgaaaaccgaggacacagccgtgtatttctgt accacagggggtggaactaactactac IGG1 13 219 7 6 3 4 4 9 5 0 4 4 JY8QFUQ01AHHJ6 16110 productive Homsap IGHV3-30*01 F 864 88.94 193/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 2 8 8 17 8.8.17 [1.17.38.11] CARDSVPRASEAAYHFDYW in-frame + ctctggattctccttcagaagttactctttccactgggtccgccaggctccaggcaaggggctagagtgggtggctgttatttcacatgatggcattactaactattatgcagactccgtgcagggccgattcatcatctccagagacaattccaagaacacgctgtttctgcacatgaacagcctgagagttgaggacacggctgtttatttttgtgcgagagattccgtacctcgggcgtcagaggctgcctaccactttgactattggggccagggaaccctgctcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [299] a85>t,T29>S(+ - +)|t105>a,S35>R(- - -)|c108>t|t111>c|g112>t,A38>S(- + -)|a115>t,M39>F(+ - -)|g117>c,M39>F(+ - -)|a162>t|a168>t|t172>c,Y58>H(+ - -)|a186>c|g188>t,S63>I(- - -)|a191>c,N64>T(- + -)|a195>c,K65>N(+ - -)|c198>t|c201>t|a214>c,K72>Q(+ - -)|c230>t,T77>I(- - -)|a263>t,Y88>F(- + -)|a270>c,Q90>H(- + -)|c287>t,A96>V(+ - +)|g303>t|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| a85>t,T29>S(+ - +)|t105>a,S35>R(- - -)|c108>t|t111>c|g112>t,A38>S(- + -)| a115>t,M39>F(+ - -)|g117>c,M39>F(+ - -)|a162>t| a168>t|t172>c,Y58>H(+ - -)|a186>c|g188>t,S63>I(- - -)|a191>c,N64>T(- + -)|a195>c,K65>N(+ - -)| c198>t|c201>t|a214>c,K72>Q(+ - -)|c230>t,T77>I(- - -)|a263>t,Y88>F(- + -)|a270>c,Q90>H(- + -)|c287>t,A96>V(+ - +)|g303>t|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| 320 225 201 24 8 16 0 0 6 2 5 0 6 1 1 0 3 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 0 1 1 0 0 1 1 0 0 1 0 51 51 48 3 1 2 0 0 0 0 0 0 2 0 1 0 0 0 30 24 18 6 2 4 0 0 0 1 3 0 1 0 0 0 1 0 117 114 104 10 3 7 0 0 5 0 2 0 2 0 0 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattctccttcagaagttactct ttccactgggtccgccaggctccaggcaaggggctagagtgggtggctgtt atttcacatgatggcattactaac tattatgcagactccgtgcagggccgattcatcatctccagagacaattccaagaacacgctgtttctgcacatgaacagcctgagagttgaggacacggctgtttatttttgt gcgagagattccgtacctcgggcgtcagaggctgcctaccactttgactat ARDSVPRASEAAYHFDY IGHV3-30 IGHD6-19 IGHJ4 ggattctccttcagaagttactct ttccactgggtccgccaggctccaggcaaggggctagagtgggtggctgtt atttcacatgatggcattactaac tattatgcagactccgtgcagggccgattcatcatctccagagacaattccaagaacacgctgtttctgcacatgaacagcctgagagttgaggacacggctgtttatttttgt gcgagagattccgtacctcgggcgtcagaggctgcctaccactttgactat IGA1 24 213 8 16 6 10 2 14 4 4 9 7 JY8QFUQ01AHIDS 17495 productive (see comment) Homsap IGHV3-48*01 F 1008 96.31 209/217 nt 95.39 207/217 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD6-19*01 F 2 8 8 15 8.8.15 [1.17.38.11] CARDQSPGVAVAGPDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'AG' (2 nucleotides at position 58 to 59 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 84 of V-REGION: 'A' (1 nucleotides at position 157 to 157 in the user submitted sequence), (cause frameshift); ctctggattcaccttcagtagatatagtatgaactgggtccgccaggctccaggaaaAGggggctggagtgggttgcatacattagaagtagcagtagtgtcatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccAaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagatcaaagtccgggtgtagcagtggctggtcctgattactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [296] c108>a,S36>R(- - -)|c114>t|g141>a|t160>g,S54>A(- + -)|t171>a,S57>R(- - -)|t177>c|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)| c108>a,S36>R(- - -)|c114>t| g141>a|t160>g,S54>A(- + -)| t171>a,S57>R(- - -)|t177>c|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)| 320 225 217 8 3 5 1 1 2 1 0 1 0 1 0 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 1 0 0 1 0 0 0 0 0 0 51 51 49 2 1 1 0 1 0 0 0 0 0 0 0 0 0 1 30 24 20 4 1 3 1 0 1 1 0 0 0 1 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagatatagt atgaactgggtccgccaggctccaggaaaggggctggagtgggttgcatac attagaagtagcagtagtgtcata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagatcaaagtccgggtgtagcagtggctggtcctgattac ARDQSPGVAVAGPDY IGHV3-48 IGHD6-19 IGHJ4 ggattcaccttcagtagatatagt atgaactgggtccgccaggctccaggaaaggggctggagtgggttgcatac attagaagtagcagtagtgtcata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagatcaaagtccgggtgtagcagtggctggtcctgattac IGA2 8 213 5 3 3 4 2 4 1 2 1 4 JY8QFUQ01AHK2I 6276 productive Homsap IGHV3-66*01 F, or Homsap IGHV3-66*02 F or Homsap IGHV3-66*04 F 971 92.86 208/224 nt NA Homsap IGHJ4*02 F 114 70.83 34/48 nt NA 8 7 8 8.7.8 [4.17.38.11] CARGAPFYDS (see Functionality comment) in-frame - IMGT/JunctionAnalysis gives no results for this JUNCTION cgtctgcagcctctggattcattgtcaatagcaactacatgagttgggtccgccaggctccagggaaggggctggactgcgtctcagttatttatcccggtggtagcacatactatgcagactccgtgaagggccgattcaccatctccagagacatctccaagaacacactgtatcttcaaatgaacagcctgagagccgaggacacggctgtatattactgtgcgagaggggccccattttatgatagtgttaagggaaccctggtcaccgtctcttcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [280] c66>g|g68>c,C23>S(- - -)|c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g104>a,S35>N(- - -)|c120>t|g153>c,E51>D(+ - +)|g156>c,W52>C(+ - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c201>t|a223>c|a245>t,N82>I(- - -)|t246>c,N82>I(- - -)|g258>a|g303>a| c66>g|g68>c,C23>S(- - -)| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g104>a,S35>N(- - -)| c120>t|g153>c,E51>D(+ - +)|g156>c,W52>C(+ - -)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)| c201>t|a223>c|a245>t,N82>I(- - -)|t246>c,N82>I(- - -)|g258>a|g303>a| a320>g| 319 (320) 231 (232) 215 16 (17) 6 (7) 10 0 (1) 3 4 1 2 0 1 0 4 1 0 0 78 14 12 2 1 1 0 0 0 0 0 0 0 0 1 1 0 0 36 24 21 3 0 3 0 1 2 0 0 0 0 0 0 0 0 0 51 51 48 3 1 2 0 0 1 0 0 0 0 0 2 0 0 0 30 21 19 2 0 2 0 0 0 0 1 0 0 0 1 0 0 0 117 114 108 6 4 2 0 2 1 1 1 0 1 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|ggca,220-223(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cgtctgcagcctct ggattcattgtcaatagcaactac atgagttgggtccgccaggctccagggaaggggctggactgcgtctcagtt atttatcccggtggtagcaca tactatgcagactccgtgaagggccgattcaccatctccagagacatctccaagaacacactgtatcttcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgagaggggccccattttatgat ARGAPFYD IGHV3-66 IGHJ4 ggattcattgtcaatagcaactac atgagttgggtccgccaggctccagggaaggggctggactgcgtctcagtt atttatcccggtggtagcaca tactatgcagactccgtgaagggccgattcaccatctccagagacatctccaagaacacactgtatcttcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgagaggggccccattttatgat IGG2 14 210 8 6 7 10 1 4 5 0 4 5 JY8QFUQ01AHMI8 17985 productive Homsap IGHV3-7*01 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F (see comment) 213 93.75 45/48 nt Homsap IGHD2-15*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARVRGYYGGQYFDSW in-frame - Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcacctttagtagttattggatgacctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaaggaagatgggaatgataaatactatgtcgactctgtgaggggccggttcaccatctccagagacaacgccaagagctcactgtttctgcaagtgaacagcctgagagccgacgacacggctgtttattactgtgcgagagttcgaggttattacggtgggcagtactttgattcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [290] c108>t|g119>c,S40>T(+ - +)|c172>g,Q58>E(+ + -)|a186>g|g188>a,S63>N(- - -)|g192>t,E64>D(+ - +)|g204>c|a215>g,K72>R(+ + +)|a225>g|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|a271>g,M91>V(+ - -)|g291>c,E97>D(+ - +)|g303>t| c108>t| g119>c,S40>T(+ - +)| c172>g,Q58>E(+ + -)|a186>g|g188>a,S63>N(- - -)|g192>t,E64>D(+ - +)| g204>c|a215>g,K72>R(+ + +)|a225>g|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|a271>g,M91>V(+ - -)|g291>c,E97>D(+ - +)|g303>t| a320>t| 319 (320) 224 (225) 210 14 (15) 5 (6) 9 5 1 1 0 0 0 1 (2) 0 3 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 20 4 1 3 1 1 0 0 0 0 0 0 0 1 1 0 117 114 106 8 3 5 4 0 0 0 0 0 1 0 2 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtagttattgg atgacctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatgggaatgataaa tactatgtcgactctgtgaggggccggttcaccatctccagagacaacgccaagagctcactgtttctgcaagtgaacagcctgagagccgacgacacggctgtttattactgt gcgagagttcgaggttattacggtgggcagtactttgattcc ARVRGYYGGQYFDS IGHV3-7 IGHD2-15 IGHJ4 ggattcacctttagtagttattgg atgacctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatgggaatgataaa tactatgtcgactctgtgaggggccggttcaccatctccagagacaacgccaagagctcactgtttctgcaagtgaacagcctgagagccgacgacacggctgtttattactgt gcgagagttcgaggttattacggtgggcagtactttgattcc IGA1 14 213 7 7 2 8 5 6 3 2 6 3 JY8QFUQ01AHN93 20435 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 937 92.24 202/219 nt 91.32 200/219 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [2.17.38.11] CVRAGFGELSGFDCW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 37 of V-REGION: 'G' (1 nucleotides at position 26 to 26 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 47 of V-REGION: 'G' (1 nucleotides at position 57 to 57 in the user submitted sequence), (cause frameshift); gcctctggatcaccctttggttattGatggcatgagctgggtccgccaagctccggGggagggggctggagtgggtctctggtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [292] t83>c,F28>S(- - -)|c84>a,F28>S(- - -)|a85>c,T29>P(+ + -)|a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a138>g|a143>g,K48>R(+ + +)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| t83>c,F28>S(- - -)|c84>a,F28>S(- - -)|a85>c,T29>P(+ + -)|a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a138>g|a143>g,K48>R(+ + +)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 226 (227) 208 18 (19) 3 (4) 15 3 3 1 2 2 (3) 1 2 0 2 1 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 0 0 1 1 1 0 0 0 0 1 0 51 51 49 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| gcctct ggatcaccctttggttattatggc atgagctgggtccgccaagctccggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggatcaccctttggttattatggc atgagctgggtccgccaagctccggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 17 213 8 9 3 8 5 9 3 0 7 7 JY8QFUQ01AHRBA 9915 productive Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F or Homsap IGHV4-34*12 F (see comment) 691 80.75 172/213 nt NA Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD3-3*01 F 3 8 7 19 8.7.19 [1.17.38.11] CARAYTFSLFGVASHWYFDLW in-frame + Low V-REGION identity (80,75% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tctgatgggtcgttcatgggttacctctggaattggatccgccagcccccagggaaggggctggagtggattggggaaatcagtcctagcggcgtcagtaagtacaatacgtccctcaagagtcgcgttgttatgagaatggacacgtcgaagaagcaattctccctggagatcaactctgtgaccgccgcggacacggctacttattattgtgcgagagcctatactttttcactttttggagtggcctcacactggtacttcgatctctggggccgtggcaccctggtcacagtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [301] a77>c,Y26>S(+ - -)|g80>a,G27>D(- - -)|c87>g|g104>t,S35>M(- - -)|t105>g,S35>M(- - -)|t112>c,Y38>L(- - -)|a113>t,Y38>L(- - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a170>g,N57>S(- - -)|a173>c,H58>P(+ - -)|t177>c|a189>c|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|c194>g,T65>S(+ - +)|c195>t,T65>S(+ - +)|c198>g,N66>K(+ - -)|c204>t|c205>a,P69>T(+ + -)|a225>c|c228>t|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|c231>t,T77>V(- - -)|a234>g,I78>M(+ + -)|t235>a,S79>R(- - -)|c236>g,S79>R(- - -)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|c249>g|c255>g,N85>K(+ - -)|g258>a|a268>g,K90>E(+ - -)|c271>a,L91>I(+ + +)|g273>c,L91>I(+ + +)|g275>a,S92>N(- - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|c309>t| a77>c,Y26>S(+ - -)| g80>a,G27>D(- - -)|c87>g|g104>t,S35>M(- - -)|t105>g,S35>M(- - -)|t112>c,Y38>L(- - -)|a113>t,Y38>L(- - -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)| a170>g,N57>S(- - -)|a173>c,H58>P(+ - -)|t177>c|a189>c|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|c194>g,T65>S(+ - +)|c195>t,T65>S(+ - +)| c198>g,N66>K(+ - -)|c204>t|c205>a,P69>T(+ + -)|a225>c|c228>t|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|c231>t,T77>V(- - -)|a234>g,I78>M(+ + -)|t235>a,S79>R(- - -)|c236>g,S79>R(- - -)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|c249>g|c255>g,N85>K(+ - -)|g258>a|a268>g,K90>E(+ - -)|c271>a,L91>I(+ + +)|g273>c,L91>I(+ + +)|g275>a,S92>N(- - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|c309>t| g320>c| 319 (320) 220 (221) 179 41 (42) 9 (10) 32 6 6 7 3 4 2 1 1 1 (2) 6 3 1 78 3 2 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 36 24 18 6 1 5 0 1 0 1 0 0 1 0 0 1 1 1 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 21 13 8 2 6 2 0 1 1 2 0 0 0 0 1 1 0 117 114 90 24 6 18 4 4 5 1 1 2 0 1 1 4 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tct gatgggtcgttcatgggttacctc tggaattggatccgccagcccccagggaaggggctggagtggattggggaa atcagtcctagcggcgtcagt aagtacaatacgtccctcaagagtcgcgttgttatgagaatggacacgtcgaagaagcaattctccctggagatcaactctgtgaccgccgcggacacggctacttattattgt gcgagagcctatactttttcactttttggagtggcctcacactggtacttcgatctc ARAYTFSLFGVASHWYFDL IGHV4-34 IGHD3-3 IGHJ2 gatgggtcgttcatgggttacctc tggaattggatccgccagcccccagggaaggggctggagtggattggggaa atcagtcctagcggcgtcagt aagtacaatacgtccctcaagagtcgcgttgttatgagaatggacacgtcgaagaagcaattctccctggagatcaactctgtgaccgccgcggacacggctacttattattgt gcgagagcctatactttttcactttttggagtggcctcacactggtacttcgatctc IGA1 40 210 22 18 13 25 9 15 6 3 20 11 JY8QFUQ01AHSTY 3707 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 814 86.57 187/216 nt 86.11 186/216 nt Homsap IGHJ3*01 F 169 82 41/50 nt Homsap IGHD3-16*01 F 3 8 8 12 8.8.12 [1.17.37.11] CARDDGGHMTLDVW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 84 of V-REGION: 1 nucleotides (from position 155 in the user submitted sequence), (cause frameshift) gtctggattcacatttagaagctattccatgaattgggtccgccaggctccagggaaggggctggagtgggtctcagctgtcagtggtggtgggggcgccacaaactacgcggagtccgtgaagggccggttcaccatctccagagacaattccaggggacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgtgcgagagatgacggcggacacatgactttagatgtctggggccaagggacaatggtcagcgtctctccggcctccaccaagggcccatcggtcttcccccgtggcaccctcctccaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactcanttccccgaaccggtgacggtgtgcgtgactca NA IGG1 79 81 [284, 285] c75>g|c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a175>g,S59>G(+ + -)|t186>g|t189>c|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|t196>a,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|c287>t,A96>V(+ - +)|g291>a|g297>a|g301>t,V101>L(+ - +)|c309>t|a317>g,K106>R(+ + +)| c75>g| c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a175>g,S59>G(+ + -)|t186>g|t189>c|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| t196>a,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|c287>t,A96>V(+ - +)|g291>a|g297>a|g301>t,V101>L(+ - +)|c309>t| a317>g,K106>R(+ + +)| 320 224 194 30 10 20 7 4 4 3 0 2 1 1 1 4 2 1 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 21 3 1 2 0 0 0 0 0 2 0 0 0 0 1 0 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 24 17 7 2 5 3 0 0 2 0 0 0 0 1 0 0 1 117 113 97 16 6 10 3 3 3 1 0 0 1 1 0 3 1 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagct gtcagtggtggtgggggcgccaca aactacgcggagtccgtgaagggccggttcaccatctccagagacaattccaggggacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgt gcgagagatgacggcggacacatgactttagatgtc ARDDGGHMTLDV IGHV3-23 IGHD3-16 IGHJ3 ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagct gtcagtggtggtgggggcgccaca aactacgcggagtccgtgaagggccggttcaccatctccagagacaattccaggggacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgt gcgagagatgacggcggacacatgactttagatgtc IGG1 28 212 17 11 8 16 9 12 6 3 12 7 JY8QFUQ01AHTOX 19654 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F (see comment) 758 82.81 183/221 nt 82.35 182/221 nt Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD6-13*01 F 3 10 7 16 10.7.16 [1.17.38.11] CARRWARQRLVMDYFDYW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (82,81% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR2-IMGT at codon 51 of V-REGION: 'G' (1 nucleotides at position 73 to 73 in the user submitted sequence), (cause frameshift); gtctctgtgactccatcagtagtactcattactattggggctggatccggcagcccccagggaggggactggGagtgggttgggagtatccactacactgggagcacctactacaactggtccctcaagcatcgagtctctatatcggtggacacatcgagtaaccagttctccctgaggttgaggtctgtgaccgccgctgacacggctgtatactactgtgcgagacgatgggcgaggcaacggctggtcatggactactttgactactggggccagggaatcgtggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [302] t74>g|c75>t|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>c,S26>L(- - -)|g79>t,G27>C(- - -)|g83>a,G28>D(- - -)|c93>t|g104>c,S35>T(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|c114>t|c129>g|a143>g,K48>R(+ + +)|g147>a|a157>g,I53>V(+ - +)|t169>c,Y57>H(+ - -)|t171>c,Y57>H(+ - -)|t174>c|g176>c,S59>T(+ - +)|c205>t,P69>W(- - -)|c206>g,P69>W(- - -)|a220>c,S74>H(+ - -)|g221>a,S74>H(+ - -)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|c237>g|a240>g|g246>a|c249>g|a251>g,K84>S(- - -)|g252>t,K84>S(- - -)|a269>g,K90>R(+ + +)|c271>t|c276>g,S92>R(- - -)|a291>t|g303>a|t306>c| t74>g|c75>t|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>c,S26>L(- - -)| g79>t,G27>C(- - -)|g83>a,G28>D(- - -)|c93>t|g104>c,S35>T(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|c114>t| c129>g|a143>g,K48>R(+ + +)|g147>a|a157>g,I53>V(+ - +)| t169>c,Y57>H(+ - -)|t171>c,Y57>H(+ - -)|t174>c|g176>c,S59>T(+ - +)| c205>t,P69>W(- - -)|c206>g,P69>W(- - -)|a220>c,S74>H(+ - -)|g221>a,S74>H(+ - -)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|c237>g|a240>g|g246>a|c249>g|a251>g,K84>S(- - -)|g252>t,K84>S(- - -)|a269>g,K90>R(+ + +)|c271>t|c276>g,S92>R(- - -)|a291>t|g303>a|t306>c| a320>g| 319 (320) 228 (229) 190 38 (39) 15 (16) 23 5 (6) 6 7 6 2 0 2 0 2 5 2 1 78 5 0 5 2 3 0 0 2 2 0 0 0 0 0 0 0 1 36 30 23 7 2 5 0 2 2 0 1 0 0 0 1 0 1 0 51 51 47 4 2 2 2 1 0 0 0 0 0 0 0 1 0 0 30 21 17 4 1 3 0 0 0 3 0 0 0 0 1 0 0 0 117 114 96 18 8 10 3 3 3 1 1 0 2 0 0 4 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctc tgtgactccatcagtagtactcattactat tggggctggatccggcagcccccagggaggggactggagtgggttgggagt atccactacactgggagcacc tactacaactggtccctcaagcatcgagtctctatatcggtggacacatcgagtaaccagttctccctgaggttgaggtctgtgaccgccgctgacacggctgtatactactgt gcgagacgatgggcgaggcaacggctggtcatggactactttgactac ARRWARQRLVMDYFDY IGHV4-39 IGHD6-13 IGHJ4 tgtgactccatcagtagtactcattactat tggggctggatccggcagcccccagggaggggactggagtgggttgggagt atccactacactgggagcacc tactacaactggtccctcaagcatcgagtctctatatcggtggacacatcgagtaaccagttctccctgaggttgaggtctgtgaccgccgctgacacggctgtatactactgt gcgagacgatgggcgaggcaacggctggtcatggactactttgactac IGG1 33 216 20 13 11 20 9 13 10 3 12 8 JY8QFUQ01AHX1B 11622 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 990 95.39 207/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD2-15*01 F 3 8 8 15 8.8.15 [1.17.38.11] CAKLGVAATTWNFFDYW in-frame + ctctggattcacctttaccacctatgccatgagctgggtccgccaggctccagggaaggggctggaatgggtctcaactattagtggtagtggtggcaggacatactacgcagactccgtgaagggccggttctccatctccagagacaattccaagaacacactatatctgcaaatgaacagcctgagagtcgaggacacggccgtatattactgtgcaaaattgggggtggcagctactacttggaacttctttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 100 100 [294] g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|g153>a|g163>a,A55>T(- - -)|t189>c|c192>g,S64>R(- - -)|a229>t,T77>S(+ - +)|g258>a|g261>a|c287>t,A96>V(+ - +)|g315>a| g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)| g153>a|g163>a,A55>T(- - -)| t189>c|c192>g,S64>R(- - -)| a229>t,T77>S(+ - +)|g258>a|g261>a|c287>t,A96>V(+ - +)| g315>a|g319>t|a320>t| 318 (320) 223 (225) 212 11 (13) 5 (7) 6 0 5 1 1 0 0 1 (2) 0 2 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 0 0 2 0 0 0 51 51 49 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 30 24 22 2 1 1 0 0 0 1 0 0 0 0 0 1 0 0 117 114 110 4 2 2 0 2 1 0 0 0 1 0 0 0 0 0 6 (8) 6 5 1 (3) 1 (3) 0 0 1 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaccacctatgcc atgagctgggtccgccaggctccagggaaggggctggaatgggtctcaact attagtggtagtggtggcaggaca tactacgcagactccgtgaagggccggttctccatctccagagacaattccaagaacacactatatctgcaaatgaacagcctgagagtcgaggacacggccgtatattactgt gcaaaattgggggtggcagctactacttggaacttctttgactac AKLGVAATTWNFFDY IGHV3-23 IGHD2-15 IGHJ4 ggattcacctttaccacctatgcc atgagctgggtccgccaggctccagggaaggggctggaatgggtctcaact attagtggtagtggtggcaggaca tactacgcagactccgtgaagggccggttctccatctccagagacaattccaagaacacactatatctgcaaatgaacagcctgagagtcgaggacacggccgtatattactgt gcaaaattgggggtggcagctactacttggaacttctttgactac IGG1 10 213 6 4 5 8 1 2 3 1 3 3 JY8QFUQ01AHY6K 18837 productive Homsap IGHV1-3*01 F 817 87.32 186/213 nt NA Homsap IGHJ5*02 F 237 96.08 49/51 nt Homsap IGHD6-6*01 F 1 8 8 13 8.8.13 [X.17.38.11] CVRGRSPPGDWFDPW in-frame - ggatacaccttcactaattatgctctgcaatgggtgcgccgggcccccggacaaacttttgagtggctgggatggatcaactctgccaatggcaacacaaaatattctcagaagtttcagggcagagtcgccattaccagggacacatccgcgaggacaacttacatggaattgagcagtctgacatctgaagacacggcgacatattattgtgtgagaggaagaagtccgcccggcgactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [283] g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a115>c,M39>L(+ + -)|t120>a,H40>Q(- + -)|a131>g,Q44>R(+ - -)|g146>c,R49>T(- - -)|g147>t,R49>T(- - -)|c148>t,L50>F(+ - -)|a157>c,M53>L(+ + -)|g172>t,A58>S(- + -)|g176>c,G59>A(- + -)|t189>c|a204>t|c213>t|a229>g,T77>A(- - -)|c255>g,S85>R(- - -)|g259>a,A87>T(- - -)|c261>t,A87>T(- - -)|g270>a|c271>t|c279>t|g284>c,R95>T(- - -)|t300>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)|c309>t|c314>t,A105>V(+ - +)| g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a115>c,M39>L(+ + -)|t120>a,H40>Q(- + -)|a131>g,Q44>R(+ - -)|g146>c,R49>T(- - -)|g147>t,R49>T(- - -)|c148>t,L50>F(+ - -)|a157>c,M53>L(+ + -)| g172>t,A58>S(- + -)|g176>c,G59>A(- + -)|t189>c| a204>t|c213>t|a229>g,T77>A(- - -)|c255>g,S85>R(- - -)|g259>a,A87>T(- - -)|c261>t,A87>T(- - -)|g270>a|c271>t|c279>t|g284>c,R95>T(- - -)|t300>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)|c309>t| c314>t,A105>V(+ - +)|a320>g| 319 (320) 220 (221) 192 28 (29) 8 (9) 20 2 (3) 5 8 2 2 0 1 1 3 1 2 1 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 51 51 44 7 0 7 1 0 1 0 2 0 0 1 1 0 1 0 30 24 21 3 1 2 0 0 0 1 0 0 0 0 1 0 1 0 117 114 99 15 7 8 1 4 5 1 0 0 1 0 1 1 0 1 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|ta,262-263(FR3)|aa,290-291(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,11-12(FR1)|tt,62-63(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,120-121(FR2)|tt,149-150(FR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,201-202(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ggatacaccttcactaattatgct ctgcaatgggtgcgccgggcccccggacaaacttttgagtggctgggatgg atcaactctgccaatggcaacaca aaatattctcagaagtttcagggcagagtcgccattaccagggacacatccgcgaggacaacttacatggaattgagcagtctgacatctgaagacacggcgacatattattgt gtgagaggaagaagtccgcccggcgactggttcgacccc VRGRSPPGDWFDP IGHV1-3 IGHD6-6 IGHJ5 ggatacaccttcactaattatgct ctgcaatgggtgcgccgggcccccggacaaacttttgagtggctgggatgg atcaactctgccaatggcaacaca aaatattctcagaagtttcagggcagagtcgccattaccagggacacatccgcgaggacaacttacatggaattgagcagtctgacatctgaagacacggcgacatattattgt gtgagaggaagaagtccgcccggcgactggttcgacccc IGA2 27 213 16 11 12 18 4 9 7 1 15 4 JY8QFUQ01AHY6R 9671 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 954 93.55 203/217 nt NA Homsap IGHJ6*02 F 256 90.32 56/62 nt Homsap IGHD2-15*01 F 2 8 8 21 8.8.21 [1.17.38.11] CAKTGGQCSGGNCYLHYYGMDVW in-frame + ctctaaattcacctttagcaactatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtcacaggtattagtggtggtggtgaaaacacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagagcatgttgtatctgcaaatgaacagcctgagagccgaagacacggccgtattttactgtgcgaaaactggcggtcaatgcagtggtggtaactgctatttgcactactacggtatggacgtctggggccaagggaccacggtcaccgtctcctcatcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtcagggcttcttccc NA IGA1 100 100 [311] g79>a,G27>K(- - -)|g80>a,G27>K(- - -)|g107>a,S36>N(- - -)|t160>a,S54>T(+ - +)|c164>g,A55>G(- + -)|a175>g,S59>G(+ + -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>a,S64>N(- - -)|a254>g,N85>S(- - -)|c257>t,T86>M(- - -)|c259>t|g291>a|a305>t,Y102>F(- + -)| g79>a,G27>K(- - -)|g80>a,G27>K(- - -)|g107>a,S36>N(- - -)| t160>a,S54>T(+ - +)|c164>g,A55>G(- + -)| a175>g,S59>G(+ + -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>a,S64>N(- - -)| a254>g,N85>S(- - -)|c257>t,T86>M(- - -)|c259>t|g291>a|a305>t,Y102>F(- + -)| g319>a|a320>c| 318 (320) 223 (225) 209 14 (16) 2 (4) 12 2 6 (7) 2 0 0 (1) 0 1 2 0 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 3 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 0 0 0 0 0 1 0 1 0 0 30 24 20 4 0 4 1 2 0 0 0 0 0 1 0 0 0 0 117 114 109 5 2 3 1 1 2 0 0 0 1 0 0 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct aaattcacctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtcacaggt attagtggtggtggtgaaaacaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagagcatgttgtatctgcaaatgaacagcctgagagccgaagacacggccgtattttactgt gcgaaaactggcggtcaatgcagtggtggtaactgctatttgcactactacggtatggacgtc AKTGGQCSGGNCYLHYYGMDV IGHV3-23 IGHD2-15 IGHJ6 aaattcacctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtcacaggt attagtggtggtggtgaaaacaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagagcatgttgtatctgcaaatgaacagcctgagagccgaagacacggccgtattttactgt gcgaaaactggcggtcaatgcagtggtggtaactgctatttgcactactacggtatggacgtc IGA1 14 213 10 4 8 9 2 5 2 0 5 7 JY8QFUQ01AHYWT 1690 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F (see comment) 678 79.91 171/214 nt 78.97 169/214 nt Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD6-6*01 F 3 8 7 9 8.7.9 [1.17.38.11] CARLDGHFDLW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (79,91% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR3-IMGT at codon 71 of V-REGION: 'C' (1 nucleotides at position 116 to 116 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 78 of V-REGION: 'AGT' (3 nucleotides at position 137 to 139 in the user submitted sequence), (do not cause frameshift); ctctgggttcaccatcagtcactactccatggcctgggtccgccaggctccgggaaaggggctggagtgggtctcattaatttatcccggcgggagcacattccacgcagactccCgtgaggggcgggcttatcgaAGTctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgtgcgagactcgacgggcacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [277] g88>a,V30>I(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g|a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c223>g,R75>G(- - -)|a225>g,R75>G(- - -)|t226>c,F76>L(+ - -)|c228>t,F76>L(+ - -)|c230>t,T77>I(- - -)|a232>g,I78>D(- - -)|t233>a,I78>D(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| g88>a,V30>I(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g| a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c223>g,R75>G(- - -)|a225>g,R75>G(- - -)|t226>c,F76>L(+ - -)|c228>t,F76>L(+ - -)|c230>t,T77>I(- - -)|a232>g,I78>D(- - -)|t233>a,I78>D(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| g319>c|a320>t| 318 (320) 220 (222) 177 43 (45) 7 (9) 36 10 8 5 3 3 2 2 (3) 3 2 (3) 3 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 2 0 0 2 0 1 0 0 0 0 0 51 51 45 6 2 4 2 1 0 0 0 0 0 1 1 0 1 0 30 21 17 4 2 2 0 0 0 1 1 0 0 0 1 0 0 1 117 114 86 28 3 25 8 5 5 2 0 2 1 2 0 3 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcaccatcagtcactactcc atggcctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgaggggcgggcttatcgactccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc ARLDGHFDL IGHV3-53 IGHD6-6 IGHJ2 gggttcaccatcagtcactactcc atggcctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgaggggcgggcttatcgactccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc IGG1 43 210 26 17 13 21 13 22 5 2 29 7 JY8QFUQ01AI3Y4 17021 productive (see comment) Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 982 94.52 207/219 nt 93.61 205/219 nt Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD3-16*01 F 2 8 8 10 8.8.10 [2.17.38.11] CAKDFTYAFDLW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 15 to 15 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 63 to 63 in the user submitted sequence), (cause frameshift); gcgtctggattcacGgttcagtagttatggcatgcactgggtccgccaggctccaggcaaggGggctggagtgggtggcaatcatctggtatgatggaagtaatcaatattatgcagactccgtgaagggccgattcaccgtctccagagacaattccaagaacacggtgtatctacaaatgaacagcctgagagccgaggacacggctgtctattattgtgcgaaagattttacgtacgcctttgatctctggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [282] c87>g|c108>t|g163>a,V55>I(+ - +)|t165>c,V55>I(+ - +)|a168>c|a193>c,K65>Q(+ - -)|c198>t|a232>g,I78>V(+ - +)|c259>g,L87>V(+ - +)|g267>a|g303>c|c309>t|g317>a,R106>K(+ + +)| c87>g|c108>t| g163>a,V55>I(+ - +)|t165>c,V55>I(+ - +)| a168>c|a193>c,K65>Q(+ - -)| c198>t|a232>g,I78>V(+ - +)|c259>g,L87>V(+ - +)|g267>a|g303>c|c309>t| g317>a,R106>K(+ + +)| 320 227 214 13 7 6 1 3 3 1 2 0 0 0 1 2 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 2 0 0 0 1 0 0 0 0 0 0 1 0 0 51 51 49 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 30 24 22 2 1 1 0 0 0 0 2 0 0 0 0 0 0 0 117 114 108 6 4 2 1 1 2 0 0 0 0 0 1 1 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcgtct ggattcacgttcagtagttatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatc atctggtatgatggaagtaatcaa tattatgcagactccgtgaagggccgattcaccgtctccagagacaattccaagaacacggtgtatctacaaatgaacagcctgagagccgaggacacggctgtctattattgt gcgaaagattttacgtacgcctttgatctc AKDFTYAFDL IGHV3-33 IGHD3-16 IGHJ3 ggattcacgttcagtagttatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatc atctggtatgatggaagtaatcaa tattatgcagactccgtgaagggccgattcaccgtctccagagacaattccaagaacacggtgtatctacaaatgaacagcctgagagccgaggacacggctgtctattattgt gcgaaagattttacgtacgcctttgatctc IGA2 12 213 7 5 5 8 2 4 4 3 4 1 JY8QFUQ01AI4NA 8512 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 900 90.78 197/217 nt NA Homsap IGHJ4*03 F (see comment) 150 79.17 38/48 nt Homsap IGHD2-15*01 F 2 8 8 14 8.8.14 [1.17.38.11] CAKAGYSSARTHMDVW in-frame + Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) ctctggattcacctttagcaattttgccatgacctgggtccgccaggctccagggaaggggctggagtgggtctcagttgtgagtggtagtggcgatagaacagactacgcagactccgtgaagggccggttcactatctccagagacaattccaagagtacactattcctgcaaatgcacagcctgagagtcgaggacacggccgtatattactgtgcgaaagctggatatagtagtgcgcgaacccacatggacgtctggggccaaggggtcgcggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [290] g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a110>t,Y37>F(- + -)|g119>c,S40>T(+ - +)|c164>t,A55>V(+ - +)|a166>g,I56>V(+ - +)|t168>g,I56>V(+ - +)|t186>c|g188>a,G63>D(- - -)|c192>a,S64>R(- - -)|t196>g,Y66>D(- - -)|c231>t|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|g261>a|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|a274>c,N92>H(- - -)|c287>t,A96>V(+ - +)| g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a110>t,Y37>F(- + -)| g119>c,S40>T(+ - +)|c164>t,A55>V(+ - +)| a166>g,I56>V(+ - +)|t168>g,I56>V(+ - +)|t186>c|g188>a,G63>D(- - -)|c192>a,S64>R(- - -)| t196>g,Y66>D(- - -)|c231>t|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|g261>a|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|a274>c,N92>H(- - -)|c287>t,A96>V(+ - +)| a320>c| 319 (320) 224 (225) 204 20 (21) 4 (5) 16 2 4 5 2 1 (2) 1 2 0 1 0 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 1 0 0 0 1 0 0 0 0 0 51 51 49 2 0 2 0 0 1 0 0 0 0 0 1 0 0 0 30 24 19 5 1 4 1 1 0 1 0 1 0 0 0 0 0 1 117 114 104 10 3 7 1 2 3 1 1 0 1 0 0 0 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcaattttgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcagtt gtgagtggtagtggcgatagaaca gactacgcagactccgtgaagggccggttcactatctccagagacaattccaagagtacactattcctgcaaatgcacagcctgagagtcgaggacacggccgtatattactgt gcgaaagctggatatagtagtgcgcgaacccacatggacgtc AKAGYSSARTHMDV IGHV3-23 IGHD2-15 IGHJ4 ggattcacctttagcaattttgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcagtt gtgagtggtagtggcgatagaaca gactacgcagactccgtgaagggccggttcactatctccagagacaattccaagagtacactattcctgcaaatgcacagcctgagagtcgaggacacggccgtatattactgt gcgaaagctggatatagtagtgcgcgaacccacatggacgtc IGA1 20 213 13 7 9 11 4 9 3 1 9 7 JY8QFUQ01AIAC1 13569 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 946 92.24 202/219 nt 91.78 201/219 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-22*01 F 2 8 8 10 8.8.10 [2.17.38.11] CAKGYHGPFDSW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 74 of V-REGION: 'G' (1 nucleotides at position 127 to 127 in the user submitted sequence), (cause frameshift); gcctctggattcacctttggcagctatatcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcagctattagtggtaggggtgttgacacatactactcagactccgtgaggGggccggttcaccatctccagagacaacgccaagaacacgatgtatctgcaaatgaacaccctgagagacgaggacacggccgtctatttctgcgcgaaaggttaccacggcccctttgactcttggggccggggaaccctggttaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [281] a103>g,S35>G(+ + -)|g112>a,A38>I(+ - +)|c113>t,A38>I(+ - +)|t177>g,S59>R(- - -)|g188>t,G63>V(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|g202>t,A68>S(- + -)|a215>g,K72>R(+ + +)|t246>c|t247>g,S83>A(- + -)|c259>a,L87>M(+ + -)|g278>c,S93>T(+ - +)|c287>a,A96>D(- - -)|a303>c|a308>t,Y103>F(- + -)|t312>c| a103>g,S35>G(+ + -)|g112>a,A38>I(+ - +)|c113>t,A38>I(+ - +)| t177>g,S59>R(- - -)|g188>t,G63>V(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| g202>t,A68>S(- + -)|a215>g,K72>R(+ + +)|t246>c|t247>g,S83>A(- + -)|c259>a,L87>M(+ + -)|g278>c,S93>T(+ - +)|c287>a,A96>D(- - -)|a303>c|a308>t,Y103>F(- + -)|t312>c| a320>g| 319 (320) 226 (227) 209 17 (18) 3 (4) 14 3 (4) 2 1 2 1 2 1 0 1 0 2 2 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 1 1 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 1 0 0 0 0 0 0 0 0 1 1 117 114 104 10 3 7 1 0 0 2 1 2 1 0 1 0 1 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacctttggcagctatatc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagct attagtggtaggggtgttgacaca tactactcagactccgtgaggggccggttcaccatctccagagacaacgccaagaacacgatgtatctgcaaatgaacaccctgagagacgaggacacggccgtctatttctgc gcgaaaggttaccacggcccctttgactct AKGYHGPFDS IGHV3-23 IGHD3-22 IGHJ4 ggattcacctttggcagctatatc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagct attagtggtaggggtgttgacaca tactactcagactccgtgaggggccggttcaccatctccagagacaacgccaagaacacgatgtatctgcaaatgaacaccctgagagacgaggacacggccgtctatttctgc gcgaaaggttaccacggcccctttgactct IGA1 17 213 8 9 3 8 5 9 3 0 7 7 JY8QFUQ01AIAF1 6394 productive Homsap IGHV1-2*02 F 903 91.59 196/214 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD6-13*01 F 2 8 8 17 8.8.17 [0.17.38.11] CARGGTVTAGGPRIVFGYW in-frame + tggctacaccttcaccgactactatatacactgggtgcgacaggcccctggacaagggcttgagtggatgggatggatcaaccctgacggtggtagcacaaagtatgcacagaaatttcagggcagggtcgccgtgaccagggacacgtcaattagcacagcctacattgaggtgaccagactgacatctgacgacacggccgtgtattattgtgccagaggagggactgtgacagcaggtggtcctcgtattgtctttgggtactggggccagggaacgttggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [296] a81>c|g107>a,G36>D(- - -)|g117>a,M39>I(+ + -)|a175>g,N59>D(+ + -)|a184>g,S62>G(+ + -)|g190>a,G64>S(+ + -)|c198>g,N66>K(+ - -)|g210>a|a229>g,T77>A(- - -)|a232>g,M78>V(+ - -)|c249>a|c252>t|g267>t,M89>I(+ + -)|c271>g,L91>V(+ - +)|g275>c,S92>T(+ - +)|g279>a|g284>c,R95>T(- - -)|c309>t|g315>c| a81>c|g107>a,G36>D(- - -)| g117>a,M39>I(+ + -)| a175>g,N59>D(+ + -)|a184>g,S62>G(+ + -)|g190>a,G64>S(+ + -)| c198>g,N66>K(+ - -)|g210>a|a229>g,T77>A(- - -)|a232>g,M78>V(+ - -)|c249>a|c252>t|g267>t,M89>I(+ + -)|c271>g,L91>V(+ - +)|g275>c,S92>T(+ - +)|g279>a|g284>c,R95>T(- - -)|c309>t| g315>c|a320>g| 319 (320) 221 (222) 202 19 (20) 7 (8) 12 4 (5) 5 2 0 1 1 0 0 3 2 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 1 0 0 1 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 2 1 0 0 0 0 0 0 0 0 0 0 117 114 102 12 5 7 2 2 2 0 0 1 0 0 2 2 1 0 7 (8) 7 6 1 (2) 1 (2) 0 0 (1) 0 0 0 0 0 0 0 1 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggctacaccttcaccgactactat atacactgggtgcgacaggcccctggacaagggcttgagtggatgggatgg atcaaccctgacggtggtagcaca aagtatgcacagaaatttcagggcagggtcgccgtgaccagggacacgtcaattagcacagcctacattgaggtgaccagactgacatctgacgacacggccgtgtattattgt gccagaggagggactgtgacagcaggtggtcctcgtattgtctttgggtac ARGGTVTAGGPRIVFGY IGHV1-2 IGHD6-13 IGHJ4 ggctacaccttcaccgactactat atacactgggtgcgacaggcccctggacaagggcttgagtggatgggatgg atcaaccctgacggtggtagcaca aagtatgcacagaaatttcagggcagggtcgccgtgaccagggacacgtcaattagcacagcctacattgaggtgaccagactgacatctgacgacacggccgtgtattattgt gccagaggagggactgtgacagcaggtggtcctcgtattgtctttgggtac IGA1 18 213 11 7 7 13 4 5 5 1 8 4 JY8QFUQ01AIE5T 6400 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 881 90.23 194/215 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD2-15*01 F 2 8 7 9 8.7.9 [1.17.38.11] CARGGSYFWDW in-frame - tctctggtggctccaccaataataactactggacttggatccggcagcccccagggaagggaatggagtgggttgggtatgtcaattatgctgggaccaccaactacaacccctccctcaagagtcgagtcactatttcagtggactcgtccaagaaccagttctccctgagggtgaactctgtgaccgctgcggacacggccgtgtattactgtgcgagaggtggcagctacttctgggactggggccagggagtcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [273] t89>c,I30>T(- - -)|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|t109>a,Y37>N(- - -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|c148>a,L50>M(+ + -)|a157>g,I53>V(+ - +)|a166>g,I56>V(+ - +)|t169>a,Y57>N(- - -)|c174>t|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|g191>c,S64>T(+ - +)|c231>t|a234>t|a240>g|a244>t,T82>S(+ - +)|a269>g,K90>R(+ + +)|c271>g,L91>V(+ - +)|g275>a,S92>N(- - -)| t89>c,I30>T(- - -)|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|t109>a,Y37>N(- - -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|c148>a,L50>M(+ + -)|a157>g,I53>V(+ - +)| a166>g,I56>V(+ - +)|t169>a,Y57>N(- - -)|c174>t|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|g191>c,S64>T(+ - +)| c231>t|a234>t|a240>g|a244>t,T82>S(+ - +)|a269>g,K90>R(+ + +)|c271>g,L91>V(+ - +)|g275>a,S92>N(- - -)| a320>g| 319 (320) 222 (223) 201 21 (22) 4 (5) 17 5 (6) 3 3 1 0 1 2 2 3 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 2 0 1 0 0 0 1 0 0 0 0 51 51 47 4 0 4 1 0 1 0 0 1 0 0 1 0 0 0 30 21 15 6 1 5 2 0 1 0 0 0 0 1 2 0 0 0 117 114 107 7 3 4 2 1 1 0 0 0 2 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccaccaataataactac tggacttggatccggcagcccccagggaagggaatggagtgggttgggtat gtcaattatgctgggaccacc aactacaacccctccctcaagagtcgagtcactatttcagtggactcgtccaagaaccagttctccctgagggtgaactctgtgaccgctgcggacacggccgtgtattactgt gcgagaggtggcagctacttctgggac ARGGSYFWD IGHV4-59 IGHD2-15 IGHJ4 ggtggctccaccaataataactac tggacttggatccggcagcccccagggaagggaatggagtgggttgggtat gtcaattatgctgggaccacc aactacaacccctccctcaagagtcgagtcactatttcagtggactcgtccaagaaccagttctccctgagggtgaactctgtgaccgctgcggacacggccgtgtattactgt gcgagaggtggcagctacttctgggac IGA1 21 210 12 9 6 11 6 10 3 1 8 9 JY8QFUQ01AIN7Q 9250 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 927 92.17 200/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD4-23*01 ORF 2 8 8 14 8.8.14 [1.17.38.11] CARAYSGPGPYYFDYW in-frame + ctccggattcaacttcaattactttagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattagtagtgatggtacttacatatactacgcagactcagtgaagggccgattcgccatctccagagacaacgccaaagactcactgtatctacaaatgaacatcctgagagccgaggacgcggctgtttattactgtgcgagagcctacagtggccctgggccttactactttgactattggggccagggaatcctggtcaccgtctcctccgcatccccgacagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctgtccagggcttcttccccca NA IGA1 100 89 [289] t78>c|c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a110>t,Y37>F(- + -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|a229>g,T77>A(- - -)|g252>a|a253>g,N85>D(+ + -)|g267>a|g278>t,S93>I(- - -)|a295>g,T99>A(- - -)|g303>t| t78>c| c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a110>t,Y37>F(- + -)| a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)| a229>g,T77>A(- - -)|g252>a|a253>g,N85>D(+ + -)|g267>a|g278>t,S93>I(- - -)|a295>g,T99>A(- - -)|g303>t| a320>c| 319 (320) 224 (225) 207 17 (18) 4 (5) 13 5 5 0 1 0 (1) 1 2 0 1 0 2 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 2 0 0 0 1 2 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 2 1 0 0 0 0 0 0 1 0 0 0 117 114 107 7 3 4 3 2 0 0 0 0 0 0 0 0 2 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcaacttcaattactttagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtgatggtacttacata tactacgcagactcagtgaagggccgattcgccatctccagagacaacgccaaagactcactgtatctacaaatgaacatcctgagagccgaggacgcggctgtttattactgt gcgagagcctacagtggccctgggccttactactttgactat ARAYSGPGPYYFDY IGHV3-21 IGHD4-23 IGHJ4 ggattcaacttcaattactttagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtgatggtacttacata tactacgcagactcagtgaagggccgattcgccatctccagagacaacgccaaagactcactgtatctacaaatgaacatcctgagagccgaggacgcggctgtttattactgt gcgagagcctacagtggccctgggccttactactttgactat IGA1 16 213 10 6 5 9 5 7 3 0 4 9 JY8QFUQ01AITB3 14516 productive (see comment) Homsap IGHV4-4*02 F, or Homsap IGHV4-4*03 F or Homsap IGHV4-4*04 F or Homsap IGHV4-4*05 F 954 93.55 203/217 nt 93.09 202/217 nt Homsap IGHJ1*01 F 224 92.31 48/52 nt Homsap IGHD4-23*01 ORF 2 9 6 10 9.6.10 [1.17.38.11] CASHGGKYFPQW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 59 of V-REGION: 1 nucleotides (from position 93 in the user submitted sequence), (cause frameshift) tctctggtggctccatcagcagtagtaactggtggagttgggtccgccagcccccagggaaggggctggagtggattggacaaatccatcatgtgggggcaccaattacaacccgtccctcgagagtcgagtcactatatcagtagacaagtccaagaaccacctctccctgaccctgaactctgtgaccgccgcggacacggccgtttatcactgtgcgagccacggtggtaaatacttcccgcagtggggccagggcaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [278] g162>a|g163>c,E55>Q(+ + -)|t169>c,Y57>H(+ - -)|a190>g,S64>G(+ + -)|c198>t|a214>g,K72>E(+ - -)|c231>t|g258>c,Q86>H(- + -)|t259>c,F87>L(+ - -)|a269>c,K90>T(- - -)|g270>c,K90>T(- - -)|g275>a,S92>N(- - -)|g303>t|t307>c,Y103>H(+ - -)| g162>a|g163>c,E55>Q(+ + -)| t169>c,Y57>H(+ - -)|a190>g,S64>G(+ + -)| c198>t|a214>g,K72>E(+ - -)|c231>t|g258>c,Q86>H(- + -)|t259>c,F87>L(+ - -)|a269>c,K90>T(- - -)|g270>c,K90>T(- - -)|g275>a,S92>N(- - -)|g303>t|t307>c,Y103>H(+ - -)| a318>c,R106>S(- - -)|g319>c| 317 (320) 222 (225) 208 (209) 14 (16) 4 (5) 10 (11) 2 2 2 3 1 (2) 0 0 0 3 (4) 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 27 27 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 30 20 18 2 0 2 1 0 0 1 0 0 0 0 0 0 0 0 117 114 104 10 3 7 1 1 2 2 1 0 0 0 2 0 1 0 5 (8) 5 5 (6) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcagtagtaactgg tggagttgggtccgccagcccccagggaaggggctggagtggattggacaa atccatcatgtgggggcacc aattacaacccgtccctcgagagtcgagtcactatatcagtagacaagtccaagaaccacctctccctgaccctgaactctgtgaccgccgcggacacggccgtttatcactgt gcgagccacggtggtaaatacttcccgcag ASHGGKYFPQ IGHV4-4 IGHD4-23 IGHJ1 ggtggctccatcagcagtagtaactgg tggagttgggtccgccagcccccagggaaggggctggagtggattggacaa atccatcatgtgggggcacc aattacaacccgtccctcgagagtcgagtcactatatcagtagacaagtccaagaaccacctctccctgaccctgaactctgtgaccgccgcggacacggccgtttatcactgt gcgagccacggtggtaaatacttcccgcag IGA2 14 212 9 5 4 8 5 6 4 0 8 2 JY8QFUQ01AITV3 13198 productive (see comment) Homsap IGHV3-49*03 F, or Homsap IGHV3-49*05 F 1039 96 216/225 nt 95.56 215/225 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 3 8 10 9 8.10.9 [2.17.38.11] CTRGGYSYAYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 62 to 62 in the user submitted sequence), (cause frameshift); acttctggattcacctttggtgattatgctatgagttggttccgccaggctccagggaaggGggctggagtgggtaggtttcattggaggcagagctcatggtggggcaacagaatacgccgcgtctgtgaaaggcagattcatcatctcaagagatgattccaaaagcatcgcctatctgcaaatgaacagcctgaaaaccgaggacacagccgtgtattattgtactagagggggatacagttatgcctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] g73>a,A25>T(- - -)|c120>t|a169>g,R57>G(- - -)|a172>g,S58>G(+ + -)|a176>g,K59>R(+ + +)|t181>c,Y61>H(+ - -)|a190>g,T64>A(- - -)|c230>t,T77>I(- - -)|c309>t| g73>a,A25>T(- - -)| c120>t| a169>g,R57>G(- - -)|a172>g,S58>G(+ + -)|a176>g,K59>R(+ + +)|t181>c,Y61>H(+ - -)|a190>g,T64>A(- - -)| c230>t,T77>I(- - -)|c309>t| a320>g| 319 (320) 232 (233) 223 9 (10) 2 (3) 7 4 (5) 1 3 1 0 0 0 0 0 0 0 0 78 6 5 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 30 25 5 0 5 4 0 0 1 0 0 0 0 0 0 0 0 117 114 112 2 1 1 0 0 2 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,158-159(FR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,181-182(CDR2)|aa,192-193(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|ta,69-70(FR1)|tt,75-76(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,124-125(FR2)|ta,158-159(FR2)|tt,162-163(FR2)|tt,167-168(CDR2)|tt,180-181(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agct,72-75(FR1)|agct,118-121(FR2)|ggtt,122-125(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggta,156-159(FR2)|ggtt,160-163(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|ggca,220-223(FR3)|agca,253-256(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,72-75(FR1)|tgct,111-114(CDR1)|agct,118-121(FR2)|agct,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| acttct ggattcacctttggtgattatgct atgagttggttccgccaggctccagggaaggggctggagtgggtaggtttc attggaggcagagctcatggtggggcaaca gaatacgccgcgtctgtgaaaggcagattcatcatctcaagagatgattccaaaagcatcgcctatctgcaaatgaacagcctgaaaaccgaggacacagccgtgtattattgt actagagggggatacagttatgcctac TRGGYSYAY IGHV3-49 IGHD5-18 IGHJ4 ggattcacctttggtgattatgct atgagttggttccgccaggctccagggaaggggctggagtgggtaggtttc attggaggcagagctcatggtggggcaaca gaatacgccgcgtctgtgaaaggcagattcatcatctcaagagatgattccaaaagcatcgcctatctgcaaatgaacagcctgaaaaccgaggacacagccgtgtattattgt actagagggggatacagttatgcctac IGA1 8 219 8 0 3 3 5 5 2 0 1 5 JY8QFUQ01AJ3EX 19882 productive Homsap IGHV3-48*01 F 1004 95.87 209/218 nt NA Homsap IGHJ3*01 F 196 88 44/50 nt Homsap IGHD3-16*01 F 3 8 8 11 8.8.11 [1.17.38.11] CARVGATLSFDVW in-frame + cctctggattcaccctcagtagctataacatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagtattagtagtggtaccatatactatgcagactctgtgaagggccgattcaccatctccagggacaatgccgagaactcactgtatctgcaaatgagcagcctgagagccgacgacacggctgtgtattactgtgcgagagtgggagcgacgctttcttttgatgtctggggccaagggacaacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 71 95 [282, 284] t88>c,F30>L(+ - -)|g113>a,S38>N(- - -)|g173>t,S58>I(- - -)|a187>g,S63>G(+ + -)|c201>t|a240>g|a250>g,K84>E(+ - -)|a275>g,N92>S(- - -)|g291>c,E97>D(+ - +)| t88>c,F30>L(+ - -)|g113>a,S38>N(- - -)| g173>t,S58>I(- - -)|a187>g,S63>G(+ + -)| c201>t|a240>g|a250>g,K84>E(+ - -)|a275>g,N92>S(- - -)|g291>c,E97>D(+ - +)| a320>t| 319 (320) 225 (226) 216 9 (10) 2 (3) 7 4 1 1 1 0 0 0 (1) 0 1 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 117 114 109 5 2 3 3 0 1 0 0 0 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccctcagtagctataac atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtattagtagtggtaccata tactatgcagactctgtgaagggccgattcaccatctccagggacaatgccgagaactcactgtatctgcaaatgagcagcctgagagccgacgacacggctgtgtattactgt gcgagagtgggagcgacgctttcttttgatgtc ARVGATLSFDV IGHV3-48 IGHD3-16 IGHJ3 ggattcaccctcagtagctataac atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtattagtagtggtaccata tactatgcagactctgtgaagggccgattcaccatctccagggacaatgccgagaactcactgtatctgcaaatgagcagcctgagagccgacgacacggctgtgtattactgt gcgagagtgggagcgacgctttcttttgatgtc IGA2 9 213 7 2 2 4 5 5 2 0 3 4 JY8QFUQ01AJ3KH 10013 productive Homsap IGHV1-2*02 F 795 85.98 184/214 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD6-19*01 F 1 8 8 13 8.8.13 [0.17.38.11] CARDWGGTYAPLDNW in-frame - tggatacattttcaccggctactatttacactgggtgcgccaggccgctggacaagggcttgagtggatgggatacatggaccctcatagtggtgacacaagctttgcaaagaaatttcagggcagggtaaccttgaccagtgacacgtccatcagtacagcctacatggaaatgagcgggctgacgtctgaggacacggccatcttttactgtgcgagagattggggtggcacgtacgctccgcttgacaattggagccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|a115>t,M39>L(+ + -)|g117>a,M39>L(+ + -)|a129>c|c136>g,P46>A(- - -)|g164>a,W55>Y(- + -)|g165>c,W55>Y(- + -)|c168>g,I56>M(+ + -)|a169>g,N57>D(+ + -)|a175>c,N59>H(- - -)|c177>t,N59>H(- - -)|g191>a,G64>D(- - -)|a197>g,N66>S(- - -)|a200>t,Y67>F(- + -)|c205>a,Q69>K(+ - -)|g210>a|c228>a|a232>t,M78>L(+ + -)|g240>t,R80>S(- - -)|c255>t|g270>a|c271>a,L91>M(+ + -)|a277>g,R93>G(- - -)|g284>c,R95>T(- - -)|a285>g,R95>T(- - -)|c291>g,D97>E(+ - +)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)|a305>t,Y102>F(- + -)| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)| a115>t,M39>L(+ + -)|g117>a,M39>L(+ + -)|a129>c|c136>g,P46>A(- - -)|g164>a,W55>Y(- + -)|g165>c,W55>Y(- + -)| c168>g,I56>M(+ + -)|a169>g,N57>D(+ + -)|a175>c,N59>H(- - -)|c177>t,N59>H(- - -)|g191>a,G64>D(- - -)| a197>g,N66>S(- - -)|a200>t,Y67>F(- + -)|c205>a,Q69>K(+ - -)|g210>a|c228>a|a232>t,M78>L(+ + -)|g240>t,R80>S(- - -)|c255>t|g270>a|c271>a,L91>M(+ + -)|a277>g,R93>G(- - -)|g284>c,R95>T(- - -)|a285>g,R95>T(- - -)|c291>g,D97>E(+ - +)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)|a305>t,Y102>F(- + -)| 320 222 192 30 5 25 4 6 4 0 2 3 4 0 3 3 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 2 0 0 0 0 0 0 0 0 0 51 51 45 6 1 5 0 2 0 0 1 0 1 0 1 1 0 0 30 24 19 5 0 5 1 1 1 0 1 0 0 0 0 1 0 0 117 114 97 17 4 13 3 3 1 0 0 3 3 0 2 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatacattttcaccggctactat ttacactgggtgcgccaggccgctggacaagggcttgagtggatgggatac atggaccctcatagtggtgacaca agctttgcaaagaaatttcagggcagggtaaccttgaccagtgacacgtccatcagtacagcctacatggaaatgagcgggctgacgtctgaggacacggccatcttttactgt gcgagagattggggtggcacgtacgctccgcttgacaat ARDWGGTYAPLDN IGHV1-2 IGHD6-19 IGHJ4 ggatacattttcaccggctactat ttacactgggtgcgccaggccgctggacaagggcttgagtggatgggatac atggaccctcatagtggtgacaca agctttgcaaagaaatttcagggcagggtaaccttgaccagtgacacgtccatcagtacagcctacatggaaatgagcgggctgacgtctgaggacacggccatcttttactgt gcgagagattggggtggcacgtacgctccgcttgacaat IGA1 30 213 14 16 10 20 4 10 5 0 18 7 JY8QFUQ01AJ891 4937 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 931 92.59 200/216 nt 92.13 199/216 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [1.16.38.11] CVRAGFGELSGFDCW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 55 of V-REGION: 1 nucleotides (from position 78 in the user submitted sequence), (cause frameshift) ctctggattcacctttggttattatggcatgagctgggtccgccaagctccggggaggggcgtggagtgggtctctgtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [287] a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a138>g|a143>g,K48>R(+ + +)|g147>c|c148>g,L50>V(+ - +)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a138>g|a143>g,K48>R(+ + +)|g147>c|c148>g,L50>V(+ - +)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 223 (224) 206 17 (18) 4 (5) 13 3 3 1 1 1 (2) 0 2 0 3 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 50 46 4 2 2 2 0 0 0 0 0 0 0 1 1 0 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ctct ggattcacctttggttattatggc atgagctgggtccgccaagctccggggaggggcgtggagtgggtctctgt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgagctgggtccgccaagctccggggaggggcgtggagtgggtctctgt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 16 212 7 9 3 9 4 7 4 0 8 4 JY8QFUQ01AJCMG 3970 productive (see comment) Homsap IGHV3-11*01 F 891 90.32 196/217 nt 89.86 195/217 nt Homsap IGHJ6*02 F 263 94.83 55/58 nt Homsap IGHD1-26*01 F 3 8 8 11 8.8.11 [1.17.38.11] CVRDYYYYGMDVW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 63 of V-REGION: 'GGG' (3 nucleotides at position 95 to 97 in the user submitted sequence), (do not cause frameshift); ttctgggttctccttcagcgactacttcatgagttgggtccgccaggctccagggaagggactggagtgggttgcatacattagtagtagtggtGGGactactaaatactacgcagactctgtgaagggccgattcaccatctccagggacaacggcaagaattcattgtttctgcaaatggacagcctgagagtcgacgacacggccatgtatttctgtgtgagagactactattattacgggatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccaggggcttcttcccccag NA IGA2 100 100 [284] c75>t|a81>g|a85>t,T29>S(+ - +)|t105>c|a113>t,Y38>F(- + -)|c120>t|a124>g,I42>V(+ - +)|g147>a|t160>g,S54>A(- + -)|g188>c,S63>T(+ - +)|c192>t|t194>a,I65>K(- + -)|c248>g,A83>G(- + -)|c255>t|c259>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>M(+ - -)|a308>t,Y103>F(- + -)|c314>t,A105>V(+ - +)| c75>t| a81>g|a85>t,T29>S(+ - +)|t105>c|a113>t,Y38>F(- + -)| c120>t|a124>g,I42>V(+ - +)|g147>a|t160>g,S54>A(- + -)| g188>c,S63>T(+ - +)|c192>t|t194>a,I65>K(- + -)| c248>g,A83>G(- + -)|c255>t|c259>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>M(+ - -)|a308>t,Y103>F(- + -)| c314>t,A105>V(+ - +)| 320 225 203 22 8 14 3 2 7 1 0 0 4 1 2 1 0 1 78 4 3 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 1 0 0 1 0 0 2 0 0 0 0 0 51 51 47 4 2 2 1 1 1 0 0 0 0 0 0 0 0 1 30 24 21 3 1 2 0 0 1 0 0 0 0 1 1 0 0 0 117 114 105 9 2 7 1 1 3 0 0 0 2 0 1 1 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ttct gggttctccttcagcgactacttc atgagttgggtccgccaggctccagggaagggactggagtgggttgcatac attagtagtagtggtactactaaa tactacgcagactctgtgaagggccgattcaccatctccagggacaacggcaagaattcattgtttctgcaaatggacagcctgagagtcgacgacacggccatgtatttctgt gtgagagactactattattacgggatggacgtc VRDYYYYGMDV IGHV3-11 IGHD1-26 IGHJ6 gggttctccttcagcgactacttc atgagttgggtccgccaggctccagggaagggactggagtgggttgcatac attagtagtagtggtactactaaa tactacgcagactctgtgaagggccgattcaccatctccagggacaacggcaagaattcattgtttctgcaaatggacagcctgagagtcgacgacacggccatgtatttctgt gtgagagactactattattacgggatggacgtc IGA2 20 213 11 9 7 10 4 10 4 3 9 4 JY8QFUQ01AJEE9 2334 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 904 91.2 197/216 nt NA Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD3-16*01 F 2 8 7 16 8.7.16 [2.17.38.11] CVRYDYSRLHRSNYFGPW in-frame - gcctctgggttcaccgtcagtagcaagttcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaattacttatcctgatggtattacatattatggagactccgtgaagggccgattcaccatctccagagacaattccaacaacacgctgtttctgcaaatgagcagcctgagagccgaggacacggccgtttattattgtgtgagatatgactactcacgcctacacagatccaactacttcggcccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [295, 294] c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)|g163>a,V55>I(+ - +)|t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>t,S64>I(- - -)|c192>t,S64>I(- - -)|c198>t|c201>t|c203>g,A68>G(- + -)|g252>c,K84>N(+ - -)|a263>t,Y88>F(- + -)|t267>g|a275>g,N92>S(- - -)|g303>t|c309>t|c314>t,A105>V(+ - +)| c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)| g163>a,V55>I(+ - +)| t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>t,S64>I(- - -)|c192>t,S64>I(- - -)| c198>t|c201>t|c203>g,A68>G(- + -)|g252>c,K84>N(+ - -)|a263>t,Y88>F(- + -)|t267>g|a275>g,N92>S(- - -)|g303>t|c309>t| c314>t,A105>V(+ - +)|g319>t| 318 (320) 222 (224) 202 (203) 20 (21) 5 (6) 15 1 2 6 1 1 0 2 0 2 2 2 (3) 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 21 14 7 0 7 0 1 2 1 1 0 0 0 1 0 1 0 117 114 105 9 5 4 1 0 3 0 0 0 1 0 1 1 1 1 6 (8) 6 5 (6) 1 (2) 0 (1) 1 0 0 1 0 0 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct gggttcaccgtcagtagcaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaatt acttatcctgatggtattaca tattatggagactccgtgaagggccgattcaccatctccagagacaattccaacaacacgctgtttctgcaaatgagcagcctgagagccgaggacacggccgtttattattgt gtgagatatgactactcacgcctacacagatccaactacttcggcccc VRYDYSRLHRSNYFGP IGHV3-53 IGHD3-16 IGHJ5 gggttcaccgtcagtagcaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaatt acttatcctgatggtattaca tattatggagactccgtgaagggccgattcaccatctccagagacaattccaacaacacgctgtttctgcaaatgagcagcctgagagccgaggacacggccgtttattattgt gtgagatatgactactcacgcctacacagatccaactacttcggcccc IGA2 19 210 9 10 7 13 2 6 5 0 5 9 JY8QFUQ01AJEL4 19653 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 932 92.2 201/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-21*02 F 3 8 8 14 8.8.14 [1.17.38.11] CAKVGVMSARDRIDYW in-frame + cctctggattcacctttagcaactatgccatgagttgggtccgccaggctcaagggaaggggctggactgggtctcagatattagtaatagtggtggtgacacattctacgcaggctccgtgaagggccgcttcaccatctccagagacaattccaggaacactctatatctgcaaatggacagcctgagagccgaggacacggccgtgtattactgtgcgaaagtgggggtgatgtccgctcgagaccgcattgactactggggccggggaaccctggtctccgtctcctcaccatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [291] g107>a,S36>N(- - -)|c120>t|c137>a,P46>Q(- - -)|g153>c,E51>D(+ - +)|c164>a,A55>D(- - -)|g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a197>t,Y66>F(- + -)|a206>g,D69>G(- - -)|g225>c|a251>g,K84>R(+ + +)|g258>t|g261>a|a274>g,N92>D(+ + -)|a303>g| g107>a,S36>N(- - -)| c120>t|c137>a,P46>Q(- - -)|g153>c,E51>D(+ - +)|c164>a,A55>D(- - -)| g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a197>t,Y66>F(- + -)|a206>g,D69>G(- - -)|g225>c|a251>g,K84>R(+ + +)|g258>t|g261>a|a274>g,N92>D(+ + -)|a303>g| a320>t| 319 (320) 225 (226) 208 17 (18) 5 (6) 12 5 5 1 0 0 2 1 (2) 0 2 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 47 4 1 3 0 0 1 0 0 2 0 0 1 0 0 0 30 24 20 4 0 4 1 3 0 0 0 0 0 0 0 0 0 0 117 114 106 8 4 4 4 1 0 0 0 0 1 0 1 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagcaactatgcc atgagttgggtccgccaggctcaagggaaggggctggactgggtctcagat attagtaatagtggtggtgacaca ttctacgcaggctccgtgaagggccgcttcaccatctccagagacaattccaggaacactctatatctgcaaatggacagcctgagagccgaggacacggccgtgtattactgt gcgaaagtgggggtgatgtccgctcgagaccgcattgactac AKVGVMSARDRIDY IGHV3-23 IGHD2-21 IGHJ4 ggattcacctttagcaactatgcc atgagttgggtccgccaggctcaagggaaggggctggactgggtctcagat attagtaatagtggtggtgacaca ttctacgcaggctccgtgaagggccgcttcaccatctccagagacaattccaggaacactctatatctgcaaatggacagcctgagagccgaggacacggccgtgtattactgt gcgaaagtgggggtgatgtccgctcgagaccgcattgactac IGA1 17 213 11 6 6 11 5 6 5 0 7 5 JY8QFUQ01AJF1Y 9657 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 941 92.66 202/218 nt NA Homsap IGHJ4*02 F 69 60.42 29/48 nt Homsap IGHD4-17*01 F 2 8 8 12 8.8.12 [1.17.38.11] CARFGPLTRPDYWG in-frame + cctctggattcacctttaccaactatggcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtggtgaaaccacataccacgcagaatccgtgcagggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgtgcgagattcggaccacttactcgacctgactactgggggccaggggaacccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [286] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)|c164>g,A55>G(- + -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>c,S64>T(+ - +)|t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a214>c,K72>Q(+ - -)|t246>c|c257>a,T86>N(- + -)|g258>t,T86>N(- + -)|c279>t|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)| c164>g,A55>G(- + -)| g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>c,S64>T(+ - +)| t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a214>c,K72>Q(+ - -)|t246>c|c257>a,T86>N(- + -)|g258>t,T86>N(- + -)|c279>t|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| a317>g,K106>R(+ + +)|g319>t|a320>t| 316 (320) 222 (226) 206 (207) 16 (19) 2 (4) 14 (15) 0 (1) 3 1 2 1 2 1 (2) 1 2 2 1 (2) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 21 3 0 3 0 1 0 0 0 0 0 1 1 0 0 0 117 114 105 9 2 7 0 1 1 2 1 2 1 0 0 0 1 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgaaaccaca taccacgcagaatccgtgcagggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgt gcgagattcggaccacttactcgacctgactactgg ARFGPLTRPDYW IGHV3-23 IGHD4-17 IGHJ4 ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgaaaccaca taccacgcagaatccgtgcagggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgt gcgagattcggaccacttactcgacctgactactgg IGG1 16 213 6 10 4 11 2 5 2 0 8 6 JY8QFUQ01AJGDO 12249 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 864 88.94 193/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-22*01 F 2 8 8 12 8.8.12 [1.17.38.11] CACYFDSNGFFDYW in-frame + ttctggatacgtctttccctcctactggatcgcctgggtgcgccagatgcccggtaaaggcctggagtggctgggaaccatctatcccggcgactctgacaccacatacagcccgtcattccaaggccaggtcaccatgtcagtcgacacgtccgtcagcaccgcctacttgcagtggagcagcctgaaggcctcggacagcgccatttactattgtgcgtgttattttgatagtaatggtttctttgactactggggccagggaacccaggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [285] a85>g,S29>V(- - -)|g86>t,S29>V(- - -)|a103>c,T35>P(+ + -)|a106>t|g107>c|g119>c,G40>A(- + -)|g141>t|a157>c,M53>L(+ + -)|g162>a|t164>c,I55>T(- - -)|t174>c|t177>c|t192>c|g197>c,R66>T(- - -)|c210>a|c234>g,I78>M(+ + -)|c239>t,A80>V(+ - +)|a245>c,K82>T(- - -)|a250>g,I84>V(+ - +)|c265>t|c296>g,T99>S(+ - +)|g303>t,M101>I(+ + -)|t306>c|c309>t| a85>g,S29>V(- - -)|g86>t,S29>V(- - -)|a103>c,T35>P(+ + -)|a106>t|g107>c| g119>c,G40>A(- + -)|g141>t|a157>c,M53>L(+ + -)|g162>a|t164>c,I55>T(- - -)| t174>c|t177>c|t192>c| g197>c,R66>T(- - -)|c210>a|c234>g,I78>M(+ + -)|c239>t,A80>V(+ - +)|a245>c,K82>T(- - -)|a250>g,I84>V(+ - +)|c265>t|c296>g,T99>S(+ - +)|g303>t,M101>I(+ + -)|t306>c|c309>t| a316>t,R106>C(- - -)|a318>t,R106>C(- - -)|c319>t| 315 (320) 220 (225) 196 (198) 24 (27) 11 (12) 13 (15) 2 1 3 (4) 5 3 1 1 (3) 0 3 2 3 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 1 0 0 0 1 0 1 0 1 0 1 0 51 51 46 5 2 3 0 1 0 1 1 0 0 0 1 0 1 0 30 24 21 3 3 0 0 0 0 3 0 0 0 0 0 0 0 0 117 114 103 11 4 7 1 0 3 1 1 1 0 0 1 2 1 0 3 (8) 3 3 (5) 0 (3) 0 (1) 0 (2) 0 0 0 (1) 0 0 0 0 (2) 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ttct ggatacgtctttccctcctactgg atcgcctgggtgcgccagatgcccggtaaaggcctggagtggctgggaacc atctatcccggcgactctgacacc acatacagcccgtcattccaaggccaggtcaccatgtcagtcgacacgtccgtcagcaccgcctacttgcagtggagcagcctgaaggcctcggacagcgccatttactattgt gcgtgttattttgatagtaatggtttctttgactac ACYFDSNGFFDY IGHV5-51 IGHD3-22 IGHJ4 ggatacgtctttccctcctactgg atcgcctgggtgcgccagatgcccggtaaaggcctggagtggctgggaacc atctatcccggcgactctgacacc acatacagcccgtcattccaaggccaggtcaccatgtcagtcgacacgtccgtcagcaccgcctacttgcagtggagcagcctgaaggcctcggacagcgccatttactattgt gcgtgttattttgatagtaatggtttctttgactac IGG1 24 213 11 13 4 13 7 11 6 5 10 3 JY8QFUQ01AJJ06 4155 productive Homsap IGHV3-11*01 F 801 85.71 186/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-21*02 F 2 8 8 18 8.8.18 [1.17.38.11] CAGSFCGGGCLWPTYHFENW in-frame + ctctggattcccctttaaggactactccatgaactggatccgccaggctccagggaagggactggagtggatttcatacatgagcagcactggtgagaccatatattacgcggactttgtgaagggccgattcaccatctccagggacaccgccaagaatctgttgtttctgcaaatgaattacctgcgagacgaggacacggccatgtattactgtgcgggatcattttgtggtggtggctgcttgtggcccacatatcactttgaaaactggggccagggagccctggtcaccgtctcgtcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [302] a85>c,T29>P(+ + -)|c90>t|g104>a,S35>K(- - -)|t105>g,S35>K(- - -)|a113>c,Y38>S(+ - -)|g119>a,S40>N(- - -)|g147>a|g157>a,V53>I(+ - +)|t168>g,I56>M(+ + -)|t171>c|t174>c|g176>c,S59>T(+ - +)|a187>g,S63>E(- - -)|g188>a,S63>E(- - -)|t189>g,S63>E(- - -)|c198>t|a204>g|c209>t,S70>F(- - -)|a245>c,N82>T(- + -)|c255>t|t256>c,S86>L(- - -)|c257>t,S86>L(- - -)|a258>g,S86>L(- - -)|c259>t|a263>t,Y88>F(- + -)|c276>t|a277>t,S93>Y(+ - -)|g278>a,S93>Y(+ - -)|a283>c|c287>a,A96>D(- - -)|g301>a,V101>M(+ - -)|a316>g,R106>G(- - -)| a85>c,T29>P(+ + -)|c90>t|g104>a,S35>K(- - -)|t105>g,S35>K(- - -)|a113>c,Y38>S(+ - -)| g119>a,S40>N(- - -)|g147>a|g157>a,V53>I(+ - +)| t168>g,I56>M(+ + -)|t171>c|t174>c|g176>c,S59>T(+ - +)|a187>g,S63>E(- - -)|g188>a,S63>E(- - -)|t189>g,S63>E(- - -)| c198>t|a204>g|c209>t,S70>F(- - -)|a245>c,N82>T(- + -)|c255>t|t256>c,S86>L(- - -)|c257>t,S86>L(- - -)|a258>g,S86>L(- - -)|c259>t|a263>t,Y88>F(- + -)|c276>t|a277>t,S93>Y(+ - -)|g278>a,S93>Y(+ - -)|a283>c|c287>a,A96>D(- - -)|g301>a,V101>M(+ - -)| a316>g,R106>G(- - -)|g319>t|a320>c| 318 (320) 223 (225) 191 32 (34) 10 (12) 22 4 7 7 3 4 (5) 1 2 0 1 0 0 (1) 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 1 0 2 0 0 0 0 0 0 1 51 51 48 3 1 2 0 3 0 0 0 0 0 0 0 0 0 0 30 24 17 7 2 5 1 1 0 2 0 0 0 0 1 0 0 2 117 114 98 16 6 10 2 2 6 1 2 1 2 0 0 0 0 0 6 (8) 6 5 1 (3) 0 (2) 1 1 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcccctttaaggactactcc atgaactggatccgccaggctccagggaagggactggagtggatttcatac atgagcagcactggtgagaccata tattacgcggactttgtgaagggccgattcaccatctccagggacaccgccaagaatctgttgtttctgcaaatgaattacctgcgagacgaggacacggccatgtattactgt gcgggatcattttgtggtggtggctgcttgtggcccacatatcactttgaaaac AGSFCGGGCLWPTYHFEN IGHV3-11 IGHD2-21 IGHJ4 ggattcccctttaaggactactcc atgaactggatccgccaggctccagggaagggactggagtggatttcatac atgagcagcactggtgagaccata tattacgcggactttgtgaagggccgattcaccatctccagggacaccgccaagaatctgttgtttctgcaaatgaattacctgcgagacgaggacacggccatgtattactgt gcgggatcattttgtggtggtggctgcttgtggcccacatatcactttgaaaac IGA1 31 213 20 11 14 16 6 15 7 3 12 9 JY8QFUQ01AJOAH 6087 productive (see comment) Homsap IGHV3-7*01 F 996 95 209/220 nt 94.09 207/220 nt Homsap IGHJ3*02 F 241 98 49/50 nt Homsap IGHD3-22*01 F 2 8 8 15 8.8.15 [2.17.38.11] CARDPYNGGGYGAFDIW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'G' (1 nucleotides at position 13 to 13 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 52 of V-REGION: 'A' (1 nucleotides at position 73 to 73 in the user submitted sequence), (cause frameshift); agcctctggattGcacctttagtaataattggatgagttgggtccgccaggctccagggaaggggctggagtAgggtggccaacatcaagcaagatggaagtgagaaagtctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacgcactgtttctgcaaatgaacagcctgagagtcgaggacacggctgtgtactactgtgcgagagacccctataatggtggtggttatggtgcttttgatatctggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [298] g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>a,Y37>N(- - -)|c120>t|a168>c|t196>g,Y66>V(- - -)|a197>t,Y66>V(- - -)|t256>g,S86>A(- + -)|a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|t306>c| g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>a,Y37>N(- - -)| c120>t| a168>c| t196>g,Y66>V(- - -)|a197>t,Y66>V(- - -)|t256>g,S86>A(- + -)|a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|t306>c| 320 228 217 11 3 8 0 1 3 1 1 0 2 1 0 0 0 2 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 1 0 0 0 0 1 0 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 23 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 117 114 108 6 1 5 0 0 1 1 0 0 2 0 0 0 0 2 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct ggattcacctttagtaataattgg atgagttgggtccgccaggctccagggaaggggctggagtgggtggccaac atcaagcaagatggaagtgagaaa gtctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacgcactgtttctgcaaatgaacagcctgagagtcgaggacacggctgtgtactactgt gcgagagacccctataatggtggtggttatggtgcttttgatatc ARDPYNGGGYGAFDI IGHV3-7 IGHD3-22 IGHJ3 ggattcacctttagtaataattgg atgagttgggtccgccaggctccagggaaggggctggagtgggtggccaac atcaagcaagatggaagtgagaaa gtctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacgcactgtttctgcaaatgaacagcctgagagtcgaggacacggctgtgtactactgt gcgagagacccctataatggtggtggttatggtgcttttgatatc IGA1 11 213 5 6 4 4 1 7 2 1 5 3 JY8QFUQ01AJQ24 15272 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 900 90.78 197/217 nt NA Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD3-10*01 F 2 8 8 18 8.8.18 [1.17.38.11] CASLNTHSGPGAERSAFDIW in-frame + ctctggatttaccttcagtctctatagaatgaactgggtccgccaggctccagggaagggactggagtgggtctcatccattactagtaccagtagttacatttattatgcagacccagtgaagggccgattcaccatctccagagacaacgccaagaactcattatatctccatatgaacagcctgagagtcgaggacacggctatatattattgtgcgagtctgaacacgcactcgggaccgggggccgaaaggtctgcttttgatatctggggccacgggacaatggtcactgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtcagggcttcttccc NA IGA1 100 100 [302] c84>t|a106>c,S36>L(- - -)|g107>t,S36>L(- - -)|c114>a,S38>R(- - -)|g147>a|g170>c,S57>T(+ - +)|g176>c,S59>T(+ - +)|t177>c,S59>T(+ - +)|a195>t|c198>t|c201>t|t208>c,S70>P(+ - -)|c259>t|g261>a|g267>c|a270>t,Q90>H(- + -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| c84>t|a106>c,S36>L(- - -)|g107>t,S36>L(- - -)|c114>a,S38>R(- - -)| g147>a| g170>c,S57>T(+ - +)|g176>c,S59>T(+ - +)|t177>c,S59>T(+ - +)|a195>t| c198>t|c201>t|t208>c,S70>P(+ - -)|c259>t|g261>a|g267>c|a270>t,Q90>H(- + -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| a318>t,R106>S(- - -)|g319>c|a320>t| 317 (320) 222 (225) 202 20 (23) 9 (11) 11 (12) 0 4 6 2 1 1 2 (4) 0 3 (4) 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 1 0 1 1 0 0 0 0 1 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 0 0 0 1 0 0 1 0 2 0 0 0 117 114 103 11 6 5 0 3 5 1 0 0 1 0 1 0 0 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 0 0 0 0 0 0 (2) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatttaccttcagtctctataga atgaactgggtccgccaggctccagggaagggactggagtgggtctcatcc attactagtaccagtagttacatt tattatgcagacccagtgaagggccgattcaccatctccagagacaacgccaagaactcattatatctccatatgaacagcctgagagtcgaggacacggctatatattattgt gcgagtctgaacacgcactcgggaccgggggccgaaaggtctgcttttgatatc ASLNTHSGPGAERSAFDI IGHV3-21 IGHD3-10 IGHJ3 ggatttaccttcagtctctataga atgaactgggtccgccaggctccagggaagggactggagtgggtctcatcc attactagtaccagtagttacatt tattatgcagacccagtgaagggccgattcaccatctccagagacaacgccaagaactcattatatctccatatgaacagcctgagagtcgaggacacggctatatattattgt gcgagtctgaacacgcactcgggaccgggggccgaaaggtctgcttttgatatc IGA1 20 213 12 8 10 15 2 5 7 2 5 6 JY8QFUQ01AJRGU 5501 productive Homsap IGHV1-69*04 F, or Homsap IGHV1-69*06 F or Homsap IGHV1-69*09 F or Homsap IGHV1-69*10 F or Homsap IGHV1-69*14 F 822 87.38 187/214 nt NA Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD6-6*01 F 1 8 8 13 8.8.13 [0.17.38.11] CAIPFSSSSAWFDPW in-frame + tggaggcaccttcagcagctatgtcatcagctggctgcgacaggcccctggacaagggcttgagtggatgggacatgtcattcctatgtttggtgtatctaacctcgcgcagaggttccagggcagagtcacaataaccgcggacacatccacgaccacagcctacatggaggtgaccaggctgagatctgaagacacggccgtctattattgtgccataccattcagcagctcgtcggcctggttcgacccctggggccagggaaccctggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG4 100 88 [285] c113>t,A38>V(+ - +)|t114>c,A38>V(+ - +)|g124>c,V42>L(+ - +)|a163>c,R55>H(- - +)|g164>a,R55>H(- - +)|g165>t,R55>H(- - +)|a166>g,I56>V(+ - +)|c171>t|c177>g,I59>M(+ + -)|c184>t,L62>F(+ - -)|a190>g,I64>V(+ - +)|g193>t,A65>S(- + -)|a195>t,A65>S(- + -)|t199>c,Y67>L(- - -)|a200>t,Y67>L(- - -)|a204>g|a209>g,K70>R(+ + +)|g231>a|t234>a|a245>c,K82>T(- - -)|g254>c,S85>T(+ - +)|c271>g,L91>V(+ - +)|g275>c,S92>T(+ - +)|c279>g,S93>R(- - -)|g291>a|g303>c|c309>t| c113>t,A38>V(+ - +)|t114>c,A38>V(+ - +)| g124>c,V42>L(+ - +)|a163>c,R55>H(- - +)|g164>a,R55>H(- - +)|g165>t,R55>H(- - +)| a166>g,I56>V(+ - +)|c171>t|c177>g,I59>M(+ + -)|c184>t,L62>F(+ - -)|a190>g,I64>V(+ - +)|g193>t,A65>S(- + -)|a195>t,A65>S(- + -)| t199>c,Y67>L(- - -)|a200>t,Y67>L(- - -)|a204>g|a209>g,K70>R(+ + +)|g231>a|t234>a|a245>c,K82>T(- - -)|g254>c,S85>T(+ - +)|c271>g,L91>V(+ - +)|g275>c,S92>T(+ - +)|c279>g,S93>R(- - -)|g291>a|g303>c|c309>t| g315>c|g317>t,R106>I(- + -)|g319>c|a320>c| 314 (320) 216 (222) 189 (191) 27 (31) 7 (10) 20 (21) 4 3 4 2 2 (3) 0 2 1 4 (6) 3 2 (3) 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 1 1 0 0 0 0 0 0 0 0 51 51 47 4 0 4 0 1 0 0 1 0 0 0 1 0 1 0 30 24 17 7 1 6 2 0 2 0 0 0 1 0 0 1 1 0 117 114 100 14 6 8 2 2 1 1 1 0 1 1 3 2 0 0 2 (8) 2 2 (4) 0 (4) 0 (3) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (2) 0 0 (1) 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,162-163(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,191-192(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|tt,185-186(CDR2)|ta,189-190(CDR2)|ta,191-192(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggca,82-85(CDR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agca,192-195(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggaggcaccttcagcagctatgtc atcagctggctgcgacaggcccctggacaagggcttgagtggatgggacat gtcattcctatgtttggtgtatct aacctcgcgcagaggttccagggcagagtcacaataaccgcggacacatccacgaccacagcctacatggaggtgaccaggctgagatctgaagacacggccgtctattattgt gccataccattcagcagctcgtcggcctggttcgacccc AIPFSSSSAWFDP IGHV1-69 IGHD6-6 IGHJ5 ggaggcaccttcagcagctatgtc atcagctggctgcgacaggcccctggacaagggcttgagtggatgggacat gtcattcctatgtttggtgtatct aacctcgcgcagaggttccagggcagagtcacaataaccgcggacacatccacgaccacagcctacatggaggtgaccaggctgagatctgaagacacggccgtctattattgt gccataccattcagcagctcgtcggcctggttcgacccc IGG4 27 213 13 14 7 16 6 11 6 1 12 8 JY8QFUQ01AJRNU 13996 productive Homsap IGHV4-61*02 F 1100 100 221/221 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD2-8*01 F 2 10 7 15 10.7.15 [1.17.38.11] CARGYCSNAVCYQIMNW in-frame - tctctggtggctccatcagcagtggtagttactactggagctggatccggcagcccgccgggaagggactggagtggattgggcgtatctataccagtgggagcaccaactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggccgtgtattactgtgcgagaggatattgttctaatgctgtatgctatcaaatcatgaactggggccagggaaccctggtcaccgtctcctcagcttccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG3 100 100 [298] a320>g| 319 (320) 228 (229) 228 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcagtggtagttactac tggagctggatccggcagcccgccgggaagggactggagtggattgggcgt atctataccagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggccgtgtattactgt gcgagaggatattgttctaatgctgtatgctatcaaatcatgaac ARGYCSNAVCYQIMN IGHV4-61 IGHD2-8 IGHJ4 ggtggctccatcagcagtggtagttactac tggagctggatccggcagcccgccgggaagggactggagtggattgggcgt atctataccagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggccgtgtattactgt gcgagaggatattgttctaatgctgtatgctatcaaatcatgaac IGG3 0 216 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01AJTUX 17542 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 630 93.1 135/145 nt 92.41 134/145 nt Homsap IGHJ5*02 F 237 96.08 49/51 nt Homsap IGHD1-20*01 F 1 8 7 16 8.7.16 [1.17.14.11] CARGPVGISESVNWFDPW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 65 of V-REGION: 73 nucleotides (from position 102 in the user submitted sequence), (cause frameshift) cctctggattcaccttcagcaaccataacatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatgtattggtagtagtagtagtgacaaatgaacagcctgagagtcgaagacacggctgtgtattactgtgcgagaggtccggttgggataagtgaaagtgtgaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [224] t105>c|g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)|g113>a,S38>N(- - -)|c164>g,S55>C(- - -)|c165>t,S55>C(- - -)|a169>g,S57>G(+ + -)|t190>g,Y64>D(- - -)|c287>t,A96>V(+ - +)|g291>a| t105>c|g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)|g113>a,S38>N(- - -)| c164>g,S55>C(- - -)|c165>t,S55>C(- - -)| a169>g,S57>G(+ + -)|t190>g,Y64>D(- - -)| c287>t,A96>V(+ - +)|g291>a| a320>g| 319 (320) 152 (153) 142 10 (11) 2 (3) 8 1 (2) 3 2 2 0 0 0 0 0 1 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 2 0 2 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 1 0 0 0 0 0 0 1 0 0 30 21 19 2 0 2 1 0 0 0 0 0 0 0 0 0 0 1 117 44 42 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagcaaccataac atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatgt attggtagtagtagtagtgac aaatgaacagcctgagagtcgaagacacggctgtgtattactgt gcgagaggtccggttgggataagtgaaagtgtgaactggttcgacccc ARGPVGISESVNWFDP IGHV3-21 IGHD1-20 IGHJ5 ggattcaccttcagcaaccataac atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatgt attggtagtagtagtagtgac aaatgaacagcctgagagtcgaagacacggctgtgtattactgt gcgagaggtccggttgggataagtgaaagtgtgaactggttcgacccc IGA1 10 140 8 2 5 6 3 4 1 1 3 5 JY8QFUQ01AJUSF 9457 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F (see comment) 765 83.87 182/217 nt NA Homsap IGHJ6*02 F 238 87.1 54/62 nt Homsap IGHD6-25*01 F 1 8 8 22 8.8.22 [1.17.38.11] CARYQGDTSGYQDVFYAHYGMDVW in-frame - Low V-REGION identity (83,87% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctggattcacttttaggggctactggatgcactgggtccgtcaggttccaggtaaggcgccggagtggctcgcacgtctgaatactgatggagatagtacaagttatgcggactccgtgaagggccgcttcaccatctccagagacaacgccaggagcacattgttcctgcaaatgagcagtctgagagtcgaagacacggccatttattactgtgcacggtatcagggcgataccagtggctaccaggacgttttctacgcccactacggtatggacgtctggggccaagggacctcggtcaccgtctattcagcatcccccgaccagcccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 89 [316] c87>t|c90>t|t105>g,S35>R(- - -)|a106>g,S36>G(+ + -)|c129>t|a132>g|c134>t,A45>V(+ - +)|g141>t|g146>c,G49>A(- + -)|t149>c,L50>P(- - -)|t152>a,V51>E(- + -)|g157>c,V53>L(+ - +)|t160>g,S54>A(- + -)|a166>c,I56>L(+ + +)|t168>g,I56>L(+ + +)|g173>c,S58>T(+ - +)|g186>a|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|c192>t|c198>t|c201>t|a225>c|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|g258>a|c259>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|g291>a|t300>c|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| c87>t|c90>t|t105>g,S35>R(- - -)|a106>g,S36>G(+ + -)| c129>t|a132>g|c134>t,A45>V(+ - +)|g141>t|g146>c,G49>A(- + -)|t149>c,L50>P(- - -)|t152>a,V51>E(- + -)|g157>c,V53>L(+ - +)|t160>g,S54>A(- + -)| a166>c,I56>L(+ + +)|t168>g,I56>L(+ + +)|g173>c,S58>T(+ - +)|g186>a|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|c192>t| c198>t|c201>t|a225>c|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|g258>a|c259>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|g291>a|t300>c|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| a316>c|a318>g|g319>t| 315 (320) 220 (225) 185 (187) 35 (38) 14 (17) 21 6 (7) 5 9 3 2 (3) 0 1 1 3 0 2 (3) 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 1 0 2 0 0 0 0 0 0 0 0 1 51 51 42 9 3 6 1 0 2 1 0 0 0 1 2 0 1 1 30 24 17 7 2 5 1 2 1 0 1 0 0 0 1 0 0 1 117 114 99 15 7 8 3 3 4 2 1 0 1 0 0 0 1 0 3 (8) 3 3 (5) 0 (3) 0 (3) 0 0 (1) 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacttttaggggctactgg atgcactgggtccgtcaggttccaggtaaggcgccggagtggctcgcacgt ctgaatactgatggagatagtaca agttatgcggactccgtgaagggccgcttcaccatctccagagacaacgccaggagcacattgttcctgcaaatgagcagtctgagagtcgaagacacggccatttattactgt gcacggtatcagggcgataccagtggctaccaggacgttttctacgcccactacggtatggacgtc ARYQGDTSGYQDVFYAHYGMDV IGHV3-74 IGHD6-25 IGHJ6 ggattcacttttaggggctactgg atgcactgggtccgtcaggttccaggtaaggcgccggagtggctcgcacgt ctgaatactgatggagatagtaca agttatgcggactccgtgaagggccgcttcaccatctccagagacaacgccaggagcacattgttcctgcaaatgagcagtctgagagtcgaagacacggccatttattactgt gcacggtatcagggcgataccagtggctaccaggacgttttctacgcccactacggtatggacgtc IGA1 35 213 23 12 14 19 9 16 10 4 14 7 JY8QFUQ01AJVOK 19032 productive (see comment) Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 923 91.74 200/218 nt 91.28 199/218 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-13*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKDPFIAATGRPWYFEYW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 85 of V-REGION: 'G' (1 nucleotides at position 161 to 161 in the user submitted sequence), (cause frameshift); cctctggattcaccttcagcagctatggcatgcactggttccgccaggctccaggcaaggggctggagtgggtggcagctatctcatatgatggaattgacaaatattatgcagactccgtgaagggccgattcaccatctccagagacaatgccaaaaaGcacgctgtatctgcaattgaacagcctgagaagtgaagacactgctgattactactgtgcgaaagacccatttatagccgcaactggccgcccctggtactttgaatactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttct NA IGA1 100 100 [301] t105>c|g124>t,V42>F(+ - -)|t164>c,V55>A(+ - +)|a168>c|g188>t,S63>I(- - -)|a190>g,N64>D(+ + -)|t192>c,N64>D(+ + -)|c198>t|t247>g,S83>A(- + -)|g252>a|a271>t,M91>L(+ + -)|g286>a,A96>S(- + -)|c287>g,A96>S(- + -)|g291>a|g297>t|t302>a,V101>D(- - -)|g303>t,V101>D(- - -)|t306>c|g317>a,R106>K(+ + +)| t105>c| g124>t,V42>F(+ - -)|t164>c,V55>A(+ - +)| a168>c|g188>t,S63>I(- - -)|a190>g,N64>D(+ + -)|t192>c,N64>D(+ + -)| c198>t|t247>g,S83>A(- + -)|g252>a|a271>t,M91>L(+ + -)|g286>a,A96>S(- + -)|c287>g,A96>S(- + -)|g291>a|g297>t|t302>a,V101>D(- - -)|g303>t,V101>D(- - -)|t306>c| g317>a,R106>K(+ + +)| 320 226 207 19 7 12 1 4 1 4 1 0 1 1 0 1 4 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 0 1 0 0 0 0 0 0 1 0 30 24 20 4 1 3 1 0 0 1 1 0 0 0 0 0 1 0 117 114 103 11 5 6 0 3 1 1 0 0 1 1 0 1 2 1 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagcagctatggc atgcactggttccgccaggctccaggcaaggggctggagtgggtggcagct atctcatatgatggaattgacaaa tattatgcagactccgtgaagggccgattcaccatctccagagacaatgccaaaaacacgctgtatctgcaattgaacagcctgagaagtgaagacactgctgattactactgt gcgaaagacccatttatagccgcaactggccgcccctggtactttgaatac AKDPFIAATGRPWYFEY IGHV3-30 IGHD6-13 IGHJ4 ggattcaccttcagcagctatggc atgcactggttccgccaggctccaggcaaggggctggagtgggtggcagct atctcatatgatggaattgacaaa tattatgcagactccgtgaagggccgattcaccatctccagagacaatgccaaaaacacgctgtatctgcaattgaacagcctgagaagtgaagacactgctgattactactgt gcgaaagacccatttatagccgcaactggccgcccctggtactttgaatac IGA1 18 213 9 9 4 9 5 9 5 2 8 3 JY8QFUQ01AJWQ7 7705 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 949 93.52 202/216 nt 92.59 200/216 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [1.16.38.11] CVRAGFGELSGFDCW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 50 of V-REGION: 'G' (1 nucleotides at position 63 to 63 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 55 of V-REGION: 1 nucleotides (from position 78 in the user submitted sequence), (cause frameshift) ctctggattcacctttggttattatggcatgagctgggtccgccaagctccgggggaggggcGtggagtgggtctctgtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttcccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaaactc NA IGG1 100 100 [289, 288] a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a138>g|a142>g,K48>E(+ - -)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a138>g|a142>g,K48>E(+ - -)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 223 (224) 208 15 (16) 3 (4) 12 3 3 1 1 1 (2) 0 2 0 2 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 50 48 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ctct ggattcacctttggttattatggc atgagctgggtccgccaagctccgggggaggggctggagtgggtctctgt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgagctgggtccgccaagctccgggggaggggctggagtgggtctctgt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 14 212 7 7 3 7 4 7 3 0 7 4 JY8QFUQ01AJWSY 19791 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 1004 95.87 209/218 nt NA Homsap IGHJ4*02 F 240 100 48/48 nt Homsap IGHD3-10*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKKYHYGSGTYFYYFDYW in-frame + cctctggattcacctttagccactatgccgtgacctgggtccgccaggctccagggaagggtctggagtgggtctcaactattagtggtagtgatggtagcacgtactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaagaagtatcactatggttcggggacttacttctactactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 100 100 [300] a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|g147>t|g163>a,A55>T(- - -)|g185>a,G62>D(- - -)|a195>g|a215>g,K72>R(+ + +)| a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)| a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|g147>t|g163>a,A55>T(- - -)| g185>a,G62>D(- - -)|a195>g| a215>g,K72>R(+ + +)| a318>g|g319>a| 317 (320) 223 (226) 214 (215) 9 (11) 2 (4) 7 3 (4) 3 (4) 0 0 1 0 0 0 1 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 1 0 0 0 0 0 0 0 51 51 47 4 1 3 1 1 0 0 0 0 0 0 1 0 1 0 30 24 22 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 117 114 113 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagccactatgcc gtgacctgggtccgccaggctccagggaagggtctggagtgggtctcaact attagtggtagtgatggtagcacg tactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaagaagtatcactatggttcggggacttacttctactactttgactac AKKYHYGSGTYFYYFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcacctttagccactatgcc gtgacctgggtccgccaggctccagggaagggtctggagtgggtctcaact attagtggtagtgatggtagcacg tactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaagaagtatcactatggttcggggacttacttctactactttgactac IGA2 9 213 6 3 3 5 3 4 1 1 4 3 JY8QFUQ01AJYIB 15333 productive Homsap IGHV3-7*01 F 912 92.06 197/214 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [0.17.38.11] CVRGRDGVNFGEMDYW in-frame - tgggttcatctttagtagacattggatggcctgggtccgccaggctccagggaagaggctggagtgggtggccaacataaaacaagatggaagtctgagatactttgtggactctgtgaagggccgattcaccatctccagagacaacgccgagagctcactgtttctgcaaatggacagcctgagaggcgaggacacggctgtgtattactgtgtgaggggtagagatggtgtcaattttggcgaaatggactactggggccagggaatcctggtcagcgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] a81>g|c86>t,T29>I(- - -)|c108>a,S36>R(- - -)|t109>c,Y37>H(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g145>a,G49>R(- - -)|g171>a|g190>c,E64>L(- - -)|a191>t,E64>L(- - -)|a194>g,K65>R(+ + +)|a200>t,Y67>F(- + -)|a250>g,K84>E(+ - -)|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c287>g,A96>G(- + -)|c314>t,A105>V(+ - +)| a81>g|c86>t,T29>I(- - -)|c108>a,S36>R(- - -)|t109>c,Y37>H(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g145>a,G49>R(- - -)| g171>a|g190>c,E64>L(- - -)|a191>t,E64>L(- - -)|a194>g,K65>R(+ + +)| a200>t,Y67>F(- + -)|a250>g,K84>E(+ - -)|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c287>g,A96>G(- + -)| c314>t,A105>V(+ - +)|a318>g|a320>g| 317 (320) 219 (222) 201 (202) 18 (20) 2 (4) 16 6 (8) 2 2 1 0 1 3 0 2 1 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 1 1 0 1 0 0 0 0 0 0 51 51 48 3 0 3 1 1 0 0 0 0 0 0 1 0 0 0 30 24 20 4 1 3 1 1 0 0 0 0 1 0 1 0 0 0 117 114 108 6 0 6 3 0 0 0 0 0 2 0 0 1 0 0 5 (8) 5 4 (5) 1 (3) 0 (2) 1 0 (2) 0 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t gggttcatctttagtagacattgg atggcctgggtccgccaggctccagggaagaggctggagtgggtggccaac ataaaacaagatggaagtctgaga tactttgtggactctgtgaagggccgattcaccatctccagagacaacgccgagagctcactgtttctgcaaatggacagcctgagaggcgaggacacggctgtgtattactgt gtgaggggtagagatggtgtcaattttggcgaaatggactac VRGRDGVNFGEMDY IGHV3-7 IGHD5-24 IGHJ4 gggttcatctttagtagacattgg atggcctgggtccgccaggctccagggaagaggctggagtgggtggccaac ataaaacaagatggaagtctgaga tactttgtggactctgtgaagggccgattcaccatctccagagacaacgccgagagctcactgtttctgcaaatggacagcctgagaggcgaggacacggctgtgtattactgt gtgaggggtagagatggtgtcaattttggcgaaatggactac IGA1 17 213 10 7 3 7 7 10 0 2 9 6 JY8QFUQ01AJYWV 16084 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 918 91.71 199/217 nt NA Homsap IGHJ4*02 F (see comment) 159 81.25 39/48 nt Homsap IGHD1-26*01 F 1 8 8 15 8.8.15 [1.17.38.11] CGKPIVGATGREMPVSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcacctttagcaactatgccatgaactgggtccgccaggttccaggggaggggctggagtgggtctcagccattagtggcagtggtggtagcacattctacacagacgccttgcagggccgattcaccatctccagagacaattccaagaacacgttatatttgcaaatgaaaagcctgagagccggggacacggccgtgtattactgtgggaagccaatagtgggcgcaacaggtagagagatgcctgtttcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [293] g107>a,S36>N(- - -)|g119>a,S40>N(- - -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|t165>c|t174>c|a197>t,Y66>F(- + -)|g202>a,A68>T(- - -)|t208>g,S70>A(- + -)|g211>t,V71>L(+ - +)|a214>c,K72>Q(+ - -)|g225>a|c259>t|g261>a|c265>t|c276>a,N92>K(+ - -)|a290>g,E97>G(- - -)|a303>g|c314>g,A105>G(- + -)| g107>a,S36>N(- - -)| g119>a,S40>N(- - -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|t165>c| t174>c| a197>t,Y66>F(- + -)|g202>a,A68>T(- - -)|t208>g,S70>A(- + -)|g211>t,V71>L(+ - +)|a214>c,K72>Q(+ - -)|g225>a|c259>t|g261>a|c265>t|c276>a,N92>K(+ - -)|a290>g,E97>G(- - -)|a303>g| c314>g,A105>G(- + -)|a318>g|g319>c|a320>c| 317 (320) 222 (225) 203 19 (22) 7 (10) 12 3 (4) 5 3 2 1 (2) 1 1 0 0 (1) 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 47 4 1 3 1 1 1 1 0 0 0 0 0 0 0 0 30 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 102 12 5 7 2 3 2 0 1 1 1 0 0 0 1 1 5 (8) 5 4 1 (4) 0 (3) 1 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 1 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcaactatgcc atgaactgggtccgccaggttccaggggaggggctggagtgggtctcagcc attagtggcagtggtggtagcaca ttctacacagacgccttgcagggccgattcaccatctccagagacaattccaagaacacgttatatttgcaaatgaaaagcctgagagccggggacacggccgtgtattactgt gggaagccaatagtgggcgcaacaggtagagagatgcctgtttcc GKPIVGATGREMPVS IGHV3-23 IGHD1-26 IGHJ4 ggattcacctttagcaactatgcc atgaactgggtccgccaggttccaggggaggggctggagtgggtctcagcc attagtggcagtggtggtagcaca ttctacacagacgccttgcagggccgattcaccatctccagagacaattccaagaacacgttatatttgcaaatgaaaagcctgagagccggggacacggccgtgtattactgt gggaagccaatagtgggcgcaacaggtagagagatgcctgtttcc IGA1 18 213 13 5 8 10 5 8 6 1 10 1 JY8QFUQ01AJZDU 11523 productive Homsap IGHV3-66*02 F (see comment) 760 83.8 181/216 nt NA Homsap IGHJ6*02 F (see comment) 197 80.33 49/61 nt Homsap IGHD2-21*01 F 3 8 7 11 8.7.11 [2.17.38.11] CARHLMVYALDVW in-frame - Low V-REGION identity (83,80% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) gtctctggattcaccgtcagtgggaagtatatgagttgggtccgccaggctccaggcaaggggctggagtgggtctcagtcttatttagtactggcactgcatactacgcagactccgtgaaaggccggttcaccatctccagagacaattccaacaacaccctatatcttcagatgaacaacatcagacctgaagacgcggccacttattattgtgcgaggcatttaatggtctacgctctggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [280] c74>t,A25>V(+ - +)|a106>g,S36>G(+ + -)|c108>g,S36>G(+ + -)|c111>g,N37>K(+ - -)|c114>t|c120>t|g141>c|t165>c|a166>t,I56>L(+ + +)|t168>a,I56>L(+ + +)|a170>t,Y57>F(- + -)|c174>t|g175>a,G59>T(+ - -)|g176>c,G59>T(+ - -)|t189>c|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|a193>g,T65>A(- - -)|g216>a|a225>g|g252>c,K84>N(+ - -)|g258>c|g261>a|a270>g|g278>a,S93>N(- - -)|c280>a,L94>I(+ + +)|g282>c,L94>I(+ + +)|g286>c,A96>P(- - -)|g291>a|a295>g,T99>A(- - -)|t300>c|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|c309>t| c74>t,A25>V(+ - +)| a106>g,S36>G(+ + -)|c108>g,S36>G(+ + -)|c111>g,N37>K(+ - -)|c114>t| c120>t|g141>c|t165>c| a166>t,I56>L(+ + +)|t168>a,I56>L(+ + +)|a170>t,Y57>F(- + -)|c174>t|g175>a,G59>T(+ - -)|g176>c,G59>T(+ - -)|t189>c|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|a193>g,T65>A(- - -)| g216>a|a225>g|g252>c,K84>N(+ - -)|g258>c|g261>a|a270>g|g278>a,S93>N(- - -)|c280>a,L94>I(+ + +)|g282>c,L94>I(+ + +)|g286>c,A96>P(- - -)|g291>a|a295>g,T99>A(- - -)|t300>c|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|c309>t| a318>g| 317 (318) 221 (222) 186 35 (36) 14 (15) 21 5 (6) 6 6 4 0 1 2 1 7 2 1 0 78 6 5 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 1 0 0 0 0 0 0 2 0 0 51 51 48 3 3 0 0 0 1 1 0 0 0 0 1 0 0 0 30 21 11 10 2 8 1 1 2 1 0 0 2 1 2 0 0 0 117 114 97 17 8 9 3 5 1 2 0 1 0 0 4 0 1 0 5 (6) 5 5 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| gtctct ggattcaccgtcagtgggaagtat atgagttgggtccgccaggctccaggcaaggggctggagtgggtctcagtc ttatttagtactggcactgca tactacgcagactccgtgaaaggccggttcaccatctccagagacaattccaacaacaccctatatcttcagatgaacaacatcagacctgaagacgcggccacttattattgt gcgaggcatttaatggtctacgctctggacgtc ARHLMVYALDV IGHV3-66 IGHD2-21 IGHJ6 ggattcaccgtcagtgggaagtat atgagttgggtccgccaggctccaggcaaggggctggagtgggtctcagtc ttatttagtactggcactgca tactacgcagactccgtgaaaggccggttcaccatctccagagacaattccaacaacaccctatatcttcagatgaacaacatcagacctgaagacgcggccacttattattgt gcgaggcatttaatggtctacgctctggacgtc IGA2 34 210 20 14 11 22 9 12 11 3 9 11 JY8QFUQ01AK0SS 21584 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*06 F or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 813 86.92 186/214 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-24*01 ORF 3 8 8 13 8.8.13 [0.17.38.11] CARGDGYNFGGLDYW in-frame - tggattcaacttcataggttatggcatgcactgggtccgccaggctccaggcaaggggctagagtgggtggcagttatatcgtatgatggcaagaacatcttttatgcagactccgtgaggggccgatccatcatttccagagacgattctaagagaacactgtttctgcaaatggacagcctgagagctgaggacacggctgtctattattgtgcgaggggagatggctacaatttcgggggattagactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] c86>a,T29>N(- + -)|g104>t,S35>I(- - -)|t105>a,S35>I(- - -)|a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)|g150>a|a171>g|a186>c|g188>a,S63>K(- - -)|t189>g,S63>K(- - -)|t192>c|a194>t,K65>I(- + -)|a195>c,K65>I(- + -)|a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|a215>g,K72>R(+ + +)|t227>c,F76>S(- - -)|c230>t,T77>I(- - -)|c234>t|a244>g,N82>D(+ + -)|c249>t|a254>g,N85>R(+ - -)|c255>a,N85>R(+ - -)|g258>a|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|g303>c|c309>t| c86>a,T29>N(- + -)|g104>t,S35>I(- - -)|t105>a,S35>I(- - -)|a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)| g150>a| a171>g|a186>c|g188>a,S63>K(- - -)|t189>g,S63>K(- - -)|t192>c|a194>t,K65>I(- + -)|a195>c,K65>I(- + -)| a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|a215>g,K72>R(+ + +)|t227>c,F76>S(- - -)|c230>t,T77>I(- - -)|c234>t|a244>g,N82>D(+ + -)|c249>t|a254>g,N85>R(+ - -)|c255>a,N85>R(+ - -)|g258>a|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|g303>c|c309>t| a318>g|a320>g| 317 (320) 219 (222) 191 (192) 28 (30) 9 (11) 19 6 (8) 3 6 2 2 2 3 1 1 0 1 1 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 0 1 0 0 1 0 1 0 0 1 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 24 17 7 3 4 1 1 0 1 2 0 1 0 0 0 0 1 117 114 99 15 5 10 4 1 5 1 0 1 2 0 1 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| t ggattcaacttcataggttatggc atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcagtt atatcgtatgatggcaagaacatc ttttatgcagactccgtgaggggccgatccatcatttccagagacgattctaagagaacactgtttctgcaaatggacagcctgagagctgaggacacggctgtctattattgt gcgaggggagatggctacaatttcgggggattagactac ARGDGYNFGGLDY IGHV3-30 IGHD5-24 IGHJ4 ggattcaacttcataggttatggc atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcagtt atatcgtatgatggcaagaacatc ttttatgcagactccgtgaggggccgatccatcatttccagagacgattctaagagaacactgtttctgcaaatggacagcctgagagctgaggacacggctgtctattattgt gcgaggggagatggctacaatttcgggggattagactac IGA1 28 213 17 11 9 13 8 15 6 3 10 9 JY8QFUQ01AK1KM 12710 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 832 87.5 189/216 nt 87.04 188/216 nt Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD6-19*01 F 1 8 8 17 8.8.17 [1.16.38.11] CARDSASSGYARYDAMDVW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 58 in the user submitted sequence), (cause frameshift) ctctggattcagttttagtacacatggcatgaactgggtccgccaggctccagggaagggccggaatgggtctcattcgttaatagtggaagtagttacatctactacgcagactcagtgaggggccgattcaccatctccagagacgacgccaggaattcactgtatctgcaaatgcaccgcctgcgagtcgaggacacggctctctactattgtgcgagagacagtgcctcgtccggctacgctaggtacgacgctatggacgtctggggccaagggaccgcggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [299] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>t|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|t109>c,Y37>H(+ - -)|a112>g,S38>G(+ + -)|t149>c,L50>P(- - -)|g153>a|c164>t,S55>F(- - -)|a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|a195>c|a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|a251>g,K84>R(+ + +)|c255>t|a274>c,N92>H(- - -)|a277>c,S93>R(- - -)|a283>c|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t306>c|c309>t| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>t|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|t109>c,Y37>H(+ - -)|a112>g,S38>G(+ + -)| t149>c,L50>P(- - -)|g153>a|c164>t,S55>F(- - -)| a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|a195>c| a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|a251>g,K84>R(+ + +)|c255>t|a274>c,N92>H(- - -)|a277>c,S93>R(- - -)|a283>c|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t306>c|c309>t| 320 224 197 27 7 20 6 2 6 3 4 1 0 1 3 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 1 6 1 0 2 1 0 1 0 0 1 1 0 0 51 50 47 3 1 2 0 1 1 1 0 0 0 0 0 0 0 0 30 24 19 5 1 4 2 1 0 0 1 0 0 1 0 0 0 0 117 114 102 12 4 8 3 0 3 1 3 0 0 0 2 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagttttagtacacatggc atgaactgggtccgccaggctccagggaagggccggaatgggtctcattc gttaatagtggaagtagttacatc tactacgcagactcagtgaggggccgattcaccatctccagagacgacgccaggaattcactgtatctgcaaatgcaccgcctgcgagtcgaggacacggctctctactattgt gcgagagacagtgcctcgtccggctacgctaggtacgacgctatggacgtc ARDSASSGYARYDAMDV IGHV3-21 IGHD6-19 IGHJ6 ggattcagttttagtacacatggc atgaactgggtccgccaggctccagggaagggccggaatgggtctcattc gttaatagtggaagtagttacatc tactacgcagactcagtgaggggccgattcaccatctccagagacgacgccaggaattcactgtatctgcaaatgcaccgcctgcgagtcgaggacacggctctctactattgt gcgagagacagtgcctcgtccggctacgctaggtacgacgctatggacgtc IGG2 27 212 17 10 8 13 9 14 5 2 10 10 JY8QFUQ01AK5T8 20986 productive (see comment) Homsap IGHV4-61*01 F, or Homsap IGHV4-61*08 F 920 90.95 201/221 nt 89.59 198/221 nt Homsap IGHJ5*02 F (see comment) 147 76.47 39/51 nt Homsap IGHD1-26*01 F 3 10 7 15 10.7.15 [1.17.38.11] CARGGSGGSYWNLIRDW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 44 of V-REGION: 'G' (1 nucleotides at position 53 to 53 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 59 of V-REGION: 'AT' (2 nucleotides at position 98 to 99 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 70 of V-REGION: 'AC' (2 nucleotides at position 124 to 125 in the user submitted sequence), (cause frameshift); tctctgatgactccgtcagcagtggtcgttactactggagttgggtccggcaGgcccccagggaagggactggagtggattggtcatttctatcacaATttgggggcactaagtacaacccctACccctcgcgagtcgagtcaccatatcagtagacacgtccaagagccagttctccctgatgctgaactctgtgaccgctgcggacacggccgtatatttctgtgcgagaggtgggtccggtgggagctactggaatctgatccgggactggggccagggaaccctggtcaccgtctactcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [302] g80>a,G27>D(- - -)|g83>a,G28>D(- - -)|a106>c,S36>R(- - -)|c120>t|a124>g,I42>V(+ - +)|g162>t|t163>c,Y55>H(+ - -)|a166>t,I56>F(+ - -)|t172>c,Y58>H(+ - -)|g176>t,S59>I(- - -)|a190>g,S64>G(+ + -)|c195>t|c198>g,N66>K(+ - -)|a214>g,K72>A(- - -)|a215>c,K72>A(- - -)|a254>g,N85>S(- - -)|a269>t,K90>M(- + -)|g275>a,S92>N(- - -)|g303>a|a308>t,Y103>F(- + -)| g80>a,G27>D(- - -)|g83>a,G28>D(- - -)|a106>c,S36>R(- - -)| c120>t|a124>g,I42>V(+ - +)|g162>t|t163>c,Y55>H(+ - -)| a166>t,I56>F(+ - -)|t172>c,Y58>H(+ - -)|g176>t,S59>I(- - -)|a190>g,S64>G(+ + -)|c195>t| c198>g,N66>K(+ - -)|a214>g,K72>A(- - -)|a215>c,K72>A(- - -)|a254>g,N85>S(- - -)|a269>t,K90>M(- + -)|g275>a,S92>N(- - -)|g303>a|a308>t,Y103>F(- + -)| a320>g| 319 (320) 228 (229) 208 20 (21) 4 (5) 16 4 (5) 4 2 2 2 0 3 0 0 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 0 2 0 0 1 0 0 0 0 0 0 0 51 51 47 4 2 2 1 0 1 1 0 0 0 0 0 0 1 0 30 21 16 5 1 4 1 0 1 1 0 0 1 0 0 0 1 0 117 114 106 8 1 7 2 2 0 0 1 0 2 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct gatgactccgtcagcagtggtcgttactac tggagttgggtccggcagcccccagggaagggactggagtggattggtcat ttctatcacattgggggcact aagtacaacccctccctcgcgagtcgagtcaccatatcagtagacacgtccaagagccagttctccctgatgctgaactctgtgaccgctgcggacacggccgtatatttctgt gcgagaggtgggtccggtgggagctactggaatctgatccgggac ARGGSGGSYWNLIRD IGHV4-61 IGHD1-26 IGHJ5 gatgactccgtcagcagtggtcgttactac tggagttgggtccggcagcccccagggaagggactggagtggattggtcat ttctatcacattgggggcact aagtacaacccctccctcgcgagtcgagtcaccatatcagtagacacgtccaagagccagttctccctgatgctgaactctgtgaccgctgcggacacggccgtatatttctgt gcgagaggtgggtccggtgggagctactggaatctgatccgggac IGA2 20 216 12 8 6 9 6 11 3 1 9 7 JY8QFUQ01AK7F3 14823 productive Homsap IGHV3-11*01 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD5-18*01 F 3 8 8 18 8.8.18 [1.17.38.11] CVRRVWGYSYGSVSYYFEYW in-frame + ctctggcttcaccttcagtgactactacatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagtggaagtggaactaccatatcctacggagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcagtgtatctccagatgaacagcctgagagccgaggacacggccgtatattactgtgtgaggcgggtgtggggatacagctatggttccgtctcgtactattttgagtactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtcagggcttcttccc NA IGA1 100 100 [302] a81>c|g119>a,S40>N(- - -)|a124>g,I42>V(+ - +)|a172>g,S58>G(+ + -)|t174>a,S58>G(+ + -)|t186>a|g188>c,S63>T(+ - +)|a197>c,Y66>S(+ - -)|c203>g,A68>G(- + -)|c259>g,L87>V(+ - +)|g267>c|a270>g|g303>a|c314>t,A105>V(+ - +)| a81>c| g119>a,S40>N(- - -)|a124>g,I42>V(+ - +)| a172>g,S58>G(+ + -)|t174>a,S58>G(+ + -)|t186>a|g188>c,S63>T(+ - +)| a197>c,Y66>S(+ - -)|c203>g,A68>G(- + -)|c259>g,L87>V(+ - +)|g267>c|a270>g|g303>a| c314>t,A105>V(+ - +)|a318>g|g319>c|a320>g| 317 (320) 222 (225) 208 14 (17) 5 (8) 9 3 (5) 2 1 0 2 0 0 2 2 (3) 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 51 51 49 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 1 0 0 0 0 0 0 2 1 0 0 0 117 114 108 6 3 3 1 1 0 0 1 0 0 0 1 2 0 0 5 (8) 5 4 1 (4) 0 (3) 1 0 (2) 0 1 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggcttcaccttcagtgactactac atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtggaagtggaactaccata tcctacggagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcagtgtatctccagatgaacagcctgagagccgaggacacggccgtatattactgt gtgaggcgggtgtggggatacagctatggttccgtctcgtactattttgagtac VRRVWGYSYGSVSYYFEY IGHV3-11 IGHD5-18 IGHJ4 ggcttcaccttcagtgactactac atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtggaagtggaactaccata tcctacggagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcagtgtatctccagatgaacagcctgagagccgaggacacggccgtatattactgt gtgaggcgggtgtggggatacagctatggttccgtctcgtactattttgagtac IGA1 13 213 5 8 2 6 3 7 3 2 5 3 JY8QFUQ01AKA7I 21396 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD6-6*01 F 2 8 8 16 8.8.16 [1.17.38.11] CAFSRALTGKWIDYLDYW in-frame + ctctggattcatcttcagcaactactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtattactggtgatgggagtaacccaatctacgcggaccccgtgaagggtcgattcaccatctccagagacaacgccaagaacacactatatctgcaaatgaacagtctgagagtcgaggacacggctgtgtattactgtgcctttagtagagcactcacggggaagtggatcgactaccttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtccgcatggcctggtccagggcttcttcccc NA IGA2 71 84 [296] c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)|a170>c,N57>T(- + -)|a172>g,S58>G(+ + -)|g191>a,S64>N(- - -)|a193>c,T65>P(+ + -)|g197>t,S66>I(- - -)|t208>c,S70>P(+ - -)|c222>t|g258>a|g261>a|c287>t,A96>V(+ - +)| c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)| a170>c,N57>T(- + -)|a172>g,S58>G(+ + -)|g191>a,S64>N(- - -)|a193>c,T65>P(+ + -)| g197>t,S66>I(- - -)|t208>c,S70>P(+ - -)|c222>t|g258>a|g261>a|c287>t,A96>V(+ - +)| a315>c|a316>t,R106>F(- - -)|g317>t,R106>F(- - -)|a318>t,R106>F(- - -)|g319>a|a320>g| 314 (320) 219 (225) 206 13 (19) 4 (7) 9 (12) 1 (2) 4 (5) 3 2 2 (3) 0 0 (2) 0 0 0 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 1 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 1 0 0 2 0 0 0 0 0 0 0 117 114 108 6 3 3 0 2 2 1 0 0 0 0 0 0 1 0 2 (8) 2 2 0 (6) 0 (3) 0 (3) 0 (1) 0 (1) 0 0 0 (1) 0 0 (2) 0 0 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagcaactactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attactggtgatgggagtaaccca atctacgcggaccccgtgaagggtcgattcaccatctccagagacaacgccaagaacacactatatctgcaaatgaacagtctgagagtcgaggacacggctgtgtattactgt gcctttagtagagcactcacggggaagtggatcgactaccttgactac AFSRALTGKWIDYLDY IGHV3-74 IGHD6-6 IGHJ4 ggattcatcttcagcaactactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attactggtgatgggagtaaccca atctacgcggaccccgtgaagggtcgattcaccatctccagagacaacgccaagaacacactatatctgcaaatgaacagtctgagagtcgaggacacggctgtgtattactgt gcctttagtagagcactcacggggaagtggatcgactaccttgactac IGA2 13 213 10 3 7 8 3 5 3 1 3 6 JY8QFUQ01AKBIM 8668 productive Homsap IGHV3-48*01 F 886 90.28 195/216 nt NA Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F (see comment) 150 79.17 38/48 nt Homsap IGHD2-21*01 F 1 8 8 12 8.8.12 [1.17.38.11] CARESTWEFTPDSW in-frame + Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) tctggattcagtttcagtgactatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagtagttctagtagtaccctatattatgcagactctgtgaagggccgattcaccgtctccagagacaatgacaagagttctctgtatctgcaaatgaccggcctgagagccgaagacacggcgacttattactgtgcgagagagtcgacgtgggaatttactcctgactcctggggcccggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 100 [283] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a175>t|g176>c|a193>c,I65>L(+ + +)|c198>t|c201>t|a232>g,I78>V(+ - +)|c248>a,A83>D(- - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a258>t|a275>c,N92>T(- + -)|a277>g,S93>G(+ + -)|g291>a|t300>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| a175>t|g176>c|a193>c,I65>L(+ + +)| c198>t|c201>t|a232>g,I78>V(+ - +)|c248>a,A83>D(- - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a258>t|a275>c,N92>T(- + -)|a277>g,S93>G(+ + -)|g291>a|t300>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)| 320 224 203 21 7 14 4 3 4 1 2 1 2 0 1 1 1 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 1 0 0 0 0 0 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 2 1 0 0 0 0 1 0 1 0 1 0 0 0 117 114 100 14 5 9 3 2 3 1 1 1 1 0 0 0 1 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcagtttcagtgactatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtagttctagtagtacccta tattatgcagactctgtgaagggccgattcaccgtctccagagacaatgacaagagttctctgtatctgcaaatgaccggcctgagagccgaagacacggcgacttattactgt gcgagagagtcgacgtgggaatttactcctgactcc ARESTWEFTPDS IGHV3-48 IGHD2-21 IGHJ4 ggattcagtttcagtgactatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtagttctagtagtacccta tattatgcagactctgtgaagggccgattcaccgtctccagagacaatgacaagagttctctgtatctgcaaatgaccggcctgagagccgaagacacggcgacttattactgt gcgagagagtcgacgtgggaatttactcctgactcc IGA1 21 213 12 9 7 11 5 10 5 2 9 5 JY8QFUQ01AKC4T 7278 productive Homsap IGHV3-13*01 F 966 94.86 203/214 nt NA Homsap IGHJ5*02 F 255 100 51/51 nt Homsap IGHD3-9*01 F 1 8 7 13 8.7.13 [1.17.38.11] CARGSSKAYNWFDPW in-frame + ctctggattcacgttcagtaattacgacatgcactgggtccgccaacctagaggaagaggtctggagtgggtctcagctattggcactggtggtgacacatactatccagactccgtgaagggccgattcaccatctccagagaaaatgccaagaactccttatatcttcagatgaacagcctgagagccggggacacggctgtgtattactgtgcaagagggtcgtcaaaagcgtacaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [285] c87>g|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g133>c,A45>P(- - -)|c137>g,T46>R(- - -)|a143>g,K48>R(+ + +)|t171>c|c176>g,A59>G(- + -)|g206>a,G69>D(- - -)|g261>a|a270>g| c87>g|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g133>c,A45>P(- - -)|c137>g,T46>R(- - -)|a143>g,K48>R(+ + +)| t171>c|c176>g,A59>G(- + -)| g206>a,G69>D(- - -)|g261>a|a270>g| a320>g| 319 (320) 221 (222) 210 11 (12) 4 (5) 7 2 (3) 3 1 1 0 0 0 0 1 3 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 0 0 0 0 0 0 1 0 0 51 51 48 3 0 3 1 0 0 0 0 0 0 0 1 1 0 0 30 21 19 2 1 1 0 0 0 1 0 0 0 0 0 1 0 0 117 114 111 3 2 1 1 2 0 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|ta,135-136(FR2)|aa,141-142(FR2)|aa,143-144(FR2)|ta,165-166(FR2-CDR2)|ta,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,242-243(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,135-136(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,259-260(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|agct,162-165(FR2)|ggta,169-172(CDR2)|ggct,205-208(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,162-165(FR2)|tact,171-174(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgttcagtaattacgac atgcactgggtccgccaacctagaggaagaggtctggagtgggtctcagct attggcactggtggtgacaca tactatccagactccgtgaagggccgattcaccatctccagagaaaatgccaagaactccttatatcttcagatgaacagcctgagagccggggacacggctgtgtattactgt gcaagagggtcgtcaaaagcgtacaactggttcgacccc ARGSSKAYNWFDP IGHV3-13 IGHD3-9 IGHJ5 ggattcacgttcagtaattacgac atgcactgggtccgccaacctagaggaagaggtctggagtgggtctcagct attggcactggtggtgacaca tactatccagactccgtgaagggccgattcaccatctccagagaaaatgccaagaactccttatatcttcagatgaacagcctgagagccggggacacggctgtgtattactgt gcaagagggtcgtcaaaagcgtacaactggttcgacccc IGG2 11 210 7 4 4 8 3 3 2 2 4 3 JY8QFUQ01AKFJR 15673 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 941 92.66 202/218 nt 91.74 200/218 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 3 8 8 12 8.8.12 [1.17.38.11] CARDTGQWKDFDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'G' (1 nucleotides at position 11 to 11 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 58 of V-REGION: 'C' (1 nucleotides at position 89 to 89 in the user submitted sequence), (cause frameshift); cctctggattGcatcttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgtagtaataCcgggggggactgacacagcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgtgcaagagatacggggcagtggaaggactttgactactggggccagggaaccctggtcaccgtctccttagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccc NA IGA1 100 100 [287] c86>t,T29>I(- - -)|c159>g|t167>g,I56>S(- - -)|g173>c,S58>T(+ - +)|t174>g,S58>T(+ - +)|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| c86>t,T29>I(- - -)| c159>g| t167>g,I56>S(- - -)|g173>c,S58>T(+ - +)|t174>g,S58>T(+ - +)|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| 320 226 210 16 3 13 3 1 1 0 1 0 0 0 3 3 1 3 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 30 24 16 8 0 8 2 1 0 0 0 0 0 0 2 0 0 3 117 114 108 6 2 4 1 0 0 0 1 0 0 0 1 2 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgt agtaatacgggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac ARDTGQWKDFDY IGHV3-74 IGHD6-19 IGHJ4 ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgt agtaatacgggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac IGA1 16 213 5 11 2 9 3 7 3 0 4 9 JY8QFUQ01AKG3P 7791 productive Homsap IGHV4-61*02 F 929 91.4 202/221 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-18*01 F 1 10 7 16 10.7.16 [1.17.38.11] CARATLSDVDTPLGFDYW in-frame + tctctggtgtctcaatcacgagtggaagtcactactggaattggattcggcagcccgccgggaagggaccggagtggattgggcgtttctataccagtgggagcaccaactacaacccctccctcaagagtcgagtcaccgtatcagcagacacgtccaagaaccggctctccctgaagctgagatctttgaccgccgcagacacggctgtttattactgtgcgagagcaaccttgtctgacgtggatacacctttgggctttgactactggggccagggaatcttggtcaccgtctcctcagcatcccctgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 89 [301] g83>t,G28>V(- - -)|c87>a|g92>c,S31>T(+ - +)|c93>g,S31>T(+ - +)|t105>a|t109>c,Y37>H(+ - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|c126>t|t149>c,L50>P(- - -)|a166>t,I56>F(+ - -)|a232>g,I78>V(+ - +)|t239>c,V80>A(+ - +)|a257>g,Q86>R(+ - -)|t259>c,F87>L(+ - -)|c276>a,S92>R(- - -)|g280>t,V94>L(+ - +)|c300>t|g303>t| g83>t,G28>V(- - -)|c87>a|g92>c,S31>T(+ - +)|c93>g,S31>T(+ - +)|t105>a|t109>c,Y37>H(+ - -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|c126>t|t149>c,L50>P(- - -)| a166>t,I56>F(+ - -)| a232>g,I78>V(+ - +)|t239>c,V80>A(+ - +)|a257>g,Q86>R(+ - -)|t259>c,F87>L(+ - -)|c276>a,S92>R(- - -)|g280>t,V94>L(+ - +)|c300>t|g303>t| a320>c| 319 (320) 228 (229) 209 19 (20) 5 (6) 14 2 1 3 4 0 (1) 2 1 1 1 1 3 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 2 4 0 0 0 1 0 1 0 1 1 1 1 0 51 51 47 4 1 3 0 1 2 1 0 0 0 0 0 0 0 0 30 21 20 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 117 114 106 8 2 6 2 0 1 2 0 1 0 0 0 0 2 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgtctcaatcacgagtggaagtcactac tggaattggattcggcagcccgccgggaagggaccggagtggattgggcgt ttctataccagtgggagcacc aactacaacccctccctcaagagtcgagtcaccgtatcagcagacacgtccaagaaccggctctccctgaagctgagatctttgaccgccgcagacacggctgtttattactgt gcgagagcaaccttgtctgacgtggatacacctttgggctttgactac ARATLSDVDTPLGFDY IGHV4-61 IGHD5-18 IGHJ4 ggtgtctcaatcacgagtggaagtcactac tggaattggattcggcagcccgccgggaagggaccggagtggattgggcgt ttctataccagtgggagcacc aactacaacccctccctcaagagtcgagtcaccgtatcagcagacacgtccaagaaccggctctccctgaagctgagatctttgaccgccgcagacacggctgtttattactgt gcgagagcaaccttgtctgacgtggatacacctttgggctttgactac IGA1 19 216 10 9 4 11 6 8 3 2 9 5 JY8QFUQ01AKHC5 11896 productive Homsap IGHV3-7*01 F 927 92.17 200/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [1.17.38.11] CVRGRDGVNFGEMDYW in-frame - ctctgggttcatctttagtagacattggatggcctgggtccgccaggctccagggaagaggctggagtgggtggccaacataaaacaagatggaagtctgagatactttgtggactctgtgaagggccgattcaccatctccagagacaacgccgagagctcactgtttctgcaaatggacagcctgagaggcgaggacacggctgtgtattactgtgtgaggggtagagatggtgtcaattttggcgaaatggactactggggccagggaatcctggtcagcgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [290] a81>g|c86>t,T29>I(- - -)|c108>a,S36>R(- - -)|t109>c,Y37>H(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g145>a,G49>R(- - -)|g171>a|g190>c,E64>L(- - -)|a191>t,E64>L(- - -)|a194>g,K65>R(+ + +)|a200>t,Y67>F(- + -)|a250>g,K84>E(+ - -)|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c287>g,A96>G(- + -)|c314>t,A105>V(+ - +)| a81>g|c86>t,T29>I(- - -)|c108>a,S36>R(- - -)|t109>c,Y37>H(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g145>a,G49>R(- - -)| g171>a|g190>c,E64>L(- - -)|a191>t,E64>L(- - -)|a194>g,K65>R(+ + +)| a200>t,Y67>F(- + -)|a250>g,K84>E(+ - -)|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c287>g,A96>G(- + -)| c314>t,A105>V(+ - +)|a318>g|a320>g| 317 (320) 222 (225) 204 (205) 18 (20) 2 (4) 16 6 (8) 2 2 1 0 1 3 0 2 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 1 1 0 1 0 0 0 0 0 0 51 51 48 3 0 3 1 1 0 0 0 0 0 0 1 0 0 0 30 24 20 4 1 3 1 1 0 0 0 0 1 0 1 0 0 0 117 114 108 6 0 6 3 0 0 0 0 0 2 0 0 1 0 0 5 (8) 5 4 (5) 1 (3) 0 (2) 1 0 (2) 0 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcatctttagtagacattgg atggcctgggtccgccaggctccagggaagaggctggagtgggtggccaac ataaaacaagatggaagtctgaga tactttgtggactctgtgaagggccgattcaccatctccagagacaacgccgagagctcactgtttctgcaaatggacagcctgagaggcgaggacacggctgtgtattactgt gtgaggggtagagatggtgtcaattttggcgaaatggactac VRGRDGVNFGEMDY IGHV3-7 IGHD5-24 IGHJ4 gggttcatctttagtagacattgg atggcctgggtccgccaggctccagggaagaggctggagtgggtggccaac ataaaacaagatggaagtctgaga tactttgtggactctgtgaagggccgattcaccatctccagagacaacgccgagagctcactgtttctgcaaatggacagcctgagaggcgaggacacggctgtgtattactgt gtgaggggtagagatggtgtcaattttggcgaaatggactac IGA2 17 213 10 7 3 7 7 10 0 2 9 6 JY8QFUQ01AKHCX 14738 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 885 90.65 194/214 nt 89.72 192/214 nt Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD2-15*01 F 3 8 7 15 8.7.15 [1.17.38.11] CTRELRVAAARDGVDIW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 37 of V-REGION: 'G' (1 nucleotides at position 25 to 25 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 61 to 61 in the user submitted sequence), (cause frameshift); ctctggattcaccgtcagtaccaaGgttcatgagctgggtccgccaggctccagggaaggGggctggagtgggtctcagtaatttatcctgatggtactacacactatggagcctccgtgaggggccggttcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgtacgagagaacttcgggtggctgctgcgcgtgatggtgttgacatctggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [292] g81>a|g107>c,S36>T(+ - +)|c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)|t165>a|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|t196>c,Y66>H(+ - -)|c201>t|c203>g,A68>G(- + -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a225>g|t262>g,Y88>D(- - -)|g303>t|c309>t|g313>a,A105>T(- - -)| g81>a|g107>c,S36>T(+ - +)|c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)| t165>a| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)| t196>c,Y66>H(+ - -)|c201>t|c203>g,A68>G(- + -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a225>g|t262>g,Y88>D(- - -)|g303>t|c309>t| g313>a,A105>T(- - -)| 320 222 201 21 6 15 2 3 4 1 2 0 1 1 3 2 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 0 0 0 0 1 0 1 1 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 21 15 6 0 6 0 1 2 0 1 0 0 0 2 0 0 0 117 114 105 9 4 5 2 0 2 1 1 0 0 0 0 1 1 1 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccgtcagtaccaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagta atttatcctgatggtactaca cactatggagcctccgtgaggggccggttcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttcgggtggctgctgcgcgtgatggtgttgacatc TRELRVAAARDGVDI IGHV3-53 IGHD2-15 IGHJ3 ggattcaccgtcagtaccaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagta atttatcctgatggtactaca cactatggagcctccgtgaggggccggttcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttcgggtggctgctgcgcgtgatggtgttgacatc IGA2 20 210 9 11 6 12 3 8 5 1 5 9 JY8QFUQ01AKI3P 11019 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 803 85.07 188/221 nt 84.16 186/221 nt Homsap IGHJ6*02 F (see comment) 188 78.69 48/61 nt Homsap IGHD1-14*01 ORF 2 10 7 12 10.7.12 [1.17.38.11] CTRQGPYLYGFDVW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) in CDR1-IMGT at codon 36 of V-REGION: 'G' (1 nucleotides at position 27 to 27 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 40 of V-REGION: 'T' (1 nucleotides at position 42 to 42 in the user submitted sequence), (cause frameshift); tctctggtgtttccgtcagtaacactGattttctattggggTctgggtccgccagtccccagggaagggactggagtggattggcagtgtagattatagtgggagcgcttcctacagccctgccctcaagagtcgagtcatcatatccatagacacgtccaagaaccagttctccatagctctgagttctgtgaccgccgcagacacggctgtctattattgtacgagacagggaccctacctctacggtttcgacgtctggggccaagggaccacggtcaccgcctctccagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccca NA IGA1 100 100 [290] g83>t,G28>V(- - -)|c84>t,G28>V(- - -)|a88>g,I30>V(+ - +)|c93>t|g101>a,S34>N(- - -)|t102>c,S34>N(- - -)|g104>c,S35>T(+ - +)|g107>t,S36>I(- - -)|a110>t,Y37>F(- + -)|c114>t|a124>g,I42>V(+ - +)|c133>t,P45>S(+ - -)|g147>a|g162>c|a166>g,I56>V(+ - +)|c168>a,I56>V(+ - +)|t169>g,Y57>D(- - -)|a193>g,T65>A(- - -)|c195>t,T65>A(- - -)|a197>c,Y66>S(+ - -)|a203>g,N68>S(- - -)|g207>t|t208>g,S70>A(- + -)|c230>t,T77>I(- - -)|g238>a,V80>I(+ - +)|c265>a,L89>I(+ + +)|g267>a,L89>I(+ + +)|a268>g,K90>A(- - -)|a269>c,K90>A(- - -)|g270>t,K90>A(- - -)|c276>t|g303>c|c309>t|g313>a,A105>T(- - -)| g83>t,G28>V(- - -)|c84>t,G28>V(- - -)|a88>g,I30>V(+ - +)|c93>t|g101>a,S34>N(- - -)|t102>c,S34>N(- - -)|g104>c,S35>T(+ - +)|g107>t,S36>I(- - -)|a110>t,Y37>F(- + -)|c114>t| a124>g,I42>V(+ - +)|c133>t,P45>S(+ - -)|g147>a|g162>c| a166>g,I56>V(+ - +)|c168>a,I56>V(+ - +)|t169>g,Y57>D(- - -)|a193>g,T65>A(- - -)|c195>t,T65>A(- - -)| a197>c,Y66>S(+ - -)|a203>g,N68>S(- - -)|g207>t|t208>g,S70>A(- + -)|c230>t,T77>I(- - -)|g238>a,V80>I(+ - +)|c265>a,L89>I(+ + +)|g267>a,L89>I(+ + +)|a268>g,K90>A(- - -)|a269>c,K90>A(- - -)|g270>t,K90>A(- - -)|c276>t|g303>c|c309>t| g313>a,A105>T(- - -)| 320 229 195 34 8 26 6 5 8 1 2 2 1 0 3 0 4 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 20 10 2 8 1 1 3 1 0 0 1 0 1 0 2 0 51 51 47 4 2 2 1 1 1 0 0 0 0 0 1 0 0 0 30 21 16 5 0 5 2 0 1 0 0 1 0 0 0 0 0 1 117 114 100 14 4 10 2 2 3 0 2 1 0 0 1 0 2 1 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgtttccgtcagtaacactattttctat tggggctgggtccgccagtccccagggaagggactggagtggattggcagt gtagattatagtgggagcgct tcctacagccctgccctcaagagtcgagtcatcatatccatagacacgtccaagaaccagttctccatagctctgagttctgtgaccgccgcagacacggctgtctattattgt acgagacagggaccctacctctacggtttcgacgtc TRQGPYLYGFDV IGHV4-39 IGHD1-14 IGHJ6 ggtgtttccgtcagtaacactattttctat tggggctgggtccgccagtccccagggaagggactggagtggattggcagt gtagattatagtgggagcgct tcctacagccctgccctcaagagtcgagtcatcatatccatagacacgtccaagaaccagttctccatagctctgagttctgtgaccgccgcagacacggctgtctattattgt acgagacagggaccctacctctacggtttcgacgtc IGA1 33 216 19 14 12 21 7 12 6 2 12 13 JY8QFUQ01AKJVX 17940 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 1007 96.74 208/215 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD3-10*01 F 2 8 7 13 8.7.13 [1.17.38.11] CAREDGSGRRHFDYW in-frame + tctctggtggctccatcagtagttactactggagctggatccggcagcccccaggaaagggactggagtggattgggtatatcttttacactgggaccaccaactacaacccctccctcaagagtcgagtcaccatgtcaatagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtctattactgtgcgagagaggacgggtccggcagacgtcactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [285] g141>a|a170>t,Y57>F(- + -)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)|a234>g,I78>M(+ + -)|g238>a,V80>I(+ - +)|g303>c| g141>a| a170>t,Y57>F(- + -)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)| a234>g,I78>M(+ + -)|g238>a,V80>I(+ - +)|g303>c| 320 223 216 7 2 5 1 2 0 0 0 0 1 0 3 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 21 18 3 0 3 0 0 0 0 0 0 1 0 2 0 0 0 117 114 111 3 1 2 1 1 0 0 0 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagtagttactac tggagctggatccggcagcccccaggaaagggactggagtggattgggtat atcttttacactgggaccacc aactacaacccctccctcaagagtcgagtcaccatgtcaatagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtctattactgt gcgagagaggacgggtccggcagacgtcactttgactac AREDGSGRRHFDY IGHV4-59 IGHD3-10 IGHJ4 ggtggctccatcagtagttactac tggagctggatccggcagcccccaggaaagggactggagtggattgggtat atcttttacactgggaccacc aactacaacccctccctcaagagtcgagtcaccatgtcaatagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtctattactgt gcgagagaggacgggtccggcagacgtcactttgactac IGA2 7 210 3 4 2 5 1 2 2 0 2 3 JY8QFUQ01AKKV6 20698 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 824 86.7 189/218 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-10*02 F 3 8 8 12 8.8.12 [1.17.38.11] CVKDISRSGCFEYW in-frame + cctctggattccagtttagcaactatgccatgagctgggtccgtcaggctcctgggaaggggctggagtgggtctcaactattagtaaagacggtgtttacacctactaccccgactccgcgaagggccgggtcaccatctccagagacaattccaagaatacaatttatttgcaaatgaacagcctgacagccgaggacacggccagatattactgtgtgaaagacatttccaggtcaggctgctttgagtattggggtcagggaaccctgatcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [285] a85>c,T29>Q(- - -)|c86>a,T29>Q(- - -)|c87>g,T29>Q(- - -)|g107>a,S36>N(- - -)|c129>t|a138>t|g163>a,A55>T(- - -)|g172>a,G58>K(- - -)|g173>a,G58>K(- - -)|t174>a,G58>K(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|t177>c,S59>D(- - -)|g188>t,G63>V(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a195>c|g202>c,A68>P(- - -)|a204>c,A68>P(- - -)|t212>c,V71>A(+ - +)|t226>g,F76>V(+ - -)|c255>t|g258>a|c259>a,L87>I(+ + +)|g261>t,L87>I(+ + +)|c265>t|g284>c,R95>T(- - -)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)|c314>t,A105>V(+ - +)| a85>c,T29>Q(- - -)|c86>a,T29>Q(- - -)|c87>g,T29>Q(- - -)|g107>a,S36>N(- - -)| c129>t|a138>t|g163>a,A55>T(- - -)| g172>a,G58>K(- - -)|g173>a,G58>K(- - -)|t174>a,G58>K(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|t177>c,S59>D(- - -)|g188>t,G63>V(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a195>c| g202>c,A68>P(- - -)|a204>c,A68>P(- - -)|t212>c,V71>A(+ - +)|t226>g,F76>V(+ - -)|c255>t|g258>a|c259>a,L87>I(+ + +)|g261>t,L87>I(+ + +)|c265>t|g284>c,R95>T(- - -)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)| c314>t,A105>V(+ - +)| 320 226 196 30 6 24 1 8 4 2 3 2 2 1 2 1 2 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 0 0 1 1 0 0 0 1 0 0 51 51 48 3 2 1 0 1 1 0 0 0 1 0 0 0 0 0 30 24 14 10 1 9 1 4 0 1 1 0 1 1 0 0 1 0 117 114 102 12 3 9 0 2 2 1 1 1 0 0 2 0 1 2 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattccagtttagcaactatgcc atgagctgggtccgtcaggctcctgggaaggggctggagtgggtctcaact attagtaaagacggtgtttacacc tactaccccgactccgcgaagggccgggtcaccatctccagagacaattccaagaatacaatttatttgcaaatgaacagcctgacagccgaggacacggccagatattactgt gtgaaagacatttccaggtcaggctgctttgagtat VKDISRSGCFEY IGHV3-23 IGHD3-10 IGHJ4 ggattccagtttagcaactatgcc atgagctgggtccgtcaggctcctgggaaggggctggagtgggtctcaact attagtaaagacggtgtttacacc tactaccccgactccgcgaagggccgggtcaccatctccagagacaattccaagaatacaatttatttgcaaatgaacagcctgacagccgaggacacggccagatattactgt gtgaaagacatttccaggtcaggctgctttgagtat IGA1 29 213 14 15 11 18 3 11 5 1 10 13 JY8QFUQ01AKM0W 17981 productive Homsap IGHV3-30*01 F, or Homsap IGHV3-30*11 F 904 91.2 197/216 nt NA Homsap IGHJ4*03 F 204 91.67 44/48 nt Homsap IGHD6-6*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAREWAASHFDHW in-frame + gctggattcaccttcagtggctttgctttgcactggctccgccaggctccagacaaggggctagagtgggtgggatttacatcatttgatgggagtaacagagactacgcagactccgtgaagggccgattcacgatctccagagacaattccaagaacacactgtatctgcaaatgaacagcctgagacctgacgacacggctgtatattactgtgcgagagagtgggcagccagtcactttgaccactggggccaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [280] t76>g,S26>A(- + -)|a106>g,S36>G(+ + -)|a110>t,Y37>F(- + -)|a115>t,M39>L(+ + -)|g124>c,V42>L(+ - +)|g140>a,G47>D(- - -)|c161>g,A54>G(- + -)|g163>t,V55>F(+ - -)|t167>c,I56>T(- - -)|a173>t,Y58>F(- + -)|a186>g|t192>c|a194>g,K65>R(+ + +)|t196>g,Y66>D(- - -)|c231>g|g258>a|g286>c,A96>P(- - -)|g291>c,E97>D(+ - +)|g303>a| t76>g,S26>A(- + -)| a106>g,S36>G(+ + -)|a110>t,Y37>F(- + -)| a115>t,M39>L(+ + -)|g124>c,V42>L(+ - +)|g140>a,G47>D(- - -)|c161>g,A54>G(- + -)|g163>t,V55>F(+ - -)| t167>c,I56>T(- - -)|a173>t,Y58>F(- + -)|a186>g|t192>c|a194>g,K65>R(+ + +)| t196>g,Y66>D(- - -)|c231>g|g258>a|g286>c,A96>P(- - -)|g291>c,E97>D(+ - +)|g303>a| 320 224 205 19 5 14 3 3 0 2 0 0 3 0 3 2 1 2 78 3 2 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 36 24 22 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 51 51 46 5 0 5 0 1 0 0 0 0 1 0 1 1 1 0 30 24 19 5 2 3 2 0 0 2 0 0 1 0 0 0 0 0 117 114 108 6 3 3 0 2 0 0 0 0 0 0 2 1 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| gct ggattcaccttcagtggctttgct ttgcactggctccgccaggctccagacaaggggctagagtgggtgggattt acatcatttgatgggagtaacaga gactacgcagactccgtgaagggccgattcacgatctccagagacaattccaagaacacactgtatctgcaaatgaacagcctgagacctgacgacacggctgtatattactgt gcgagagagtgggcagccagtcactttgaccac AREWAASHFDH IGHV3-30 IGHD6-6 IGHJ4 ggattcaccttcagtggctttgct ttgcactggctccgccaggctccagacaaggggctagagtgggtgggattt acatcatttgatgggagtaacaga gactacgcagactccgtgaagggccgattcacgatctccagagacaattccaagaacacactgtatctgcaaatgaacagcctgagacctgacgacacggctgtatattactgt gcgagagagtgggcagccagtcactttgaccac IGA2 18 213 8 10 3 9 5 9 3 2 8 5 JY8QFUQ01AKNSQ 19223 productive Homsap IGHV3-7*01 F 945 93.09 202/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-12*01 F 3 8 8 10 8.8.10 [1.17.38.11] CATISDYRFEFW in-frame + ctctggattcacctttagtggctattggatgagttgggtccgccaggctccggggaagggtctggagtgggtggccaacatagagaaagatggaagtgacataaagtatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaattcactgtctctgcaaatgaacagcctgagagccgacgacacggctatttattactgtgcgaccattagtgactaccgatttgaattctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 71 95 [278, 280] a106>g,S36>G(+ + -)|c120>t|a138>g|g147>t|a169>g,K57>E(+ - -)|c172>a,Q58>K(+ - -)|g192>c,E64>D(+ - +)|a194>t,K65>I(- + -)|t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|c255>t|a263>c,Y88>S(+ - -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| a106>g,S36>G(+ + -)| c120>t|a138>g|g147>t| a169>g,K57>E(+ - -)|c172>a,Q58>K(+ - -)|g192>c,E64>D(+ - +)|a194>t,K65>I(- + -)| t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|c255>t|a263>c,Y88>S(+ - -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| g317>c,R106>T(- - -)|a318>c,R106>T(- - -)|g319>a|a320>t| 316 (320) 221 (225) 206 15 (19) 4 (6) 11 (13) 3 1 (2) 2 0 1 (2) 1 1 (2) 1 2 (3) 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 51 48 3 3 0 1 0 1 0 0 0 0 0 0 0 1 0 30 24 20 4 0 4 1 0 0 0 0 1 1 0 1 0 0 0 117 114 107 7 1 6 0 1 1 0 1 0 0 1 1 1 1 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 0 (1) 0 0 0 (1) 0 0 (1) 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtggctattgg atgagttgggtccgccaggctccggggaagggtctggagtgggtggccaac atagagaaagatggaagtgacata aagtatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaattcactgtctctgcaaatgaacagcctgagagccgacgacacggctatttattactgt gcgaccattagtgactaccgatttgaattc ATISDYRFEF IGHV3-7 IGHD5-12 IGHJ4 ggattcacctttagtggctattgg atgagttgggtccgccaggctccggggaagggtctggagtgggtggccaac atagagaaagatggaagtgacata aagtatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaattcactgtctctgcaaatgaacagcctgagagccgacgacacggctatttattactgt gcgaccattagtgactaccgatttgaattc IGA2 15 213 6 9 3 9 3 6 4 0 6 5 JY8QFUQ01AKOZJ 14010 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 1017 96.77 210/217 nt 96.31 209/217 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-21*02 F 3 7 8 20 7.8.20 [1.17.38.11] CARLPYIVVLTNIISPTYFDFW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 29 of V-REGION: 1 nucleotides (from position 14 in the user submitted sequence), (cause frameshift) tttctggatacagtttaccagttactggatcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggtatcatctatcctgctgactctgataccagatacagcccgtccttccaaggccaggtcagcatctcagccgacaagtccatcgacaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatgtattactgtgcgcggctgccatatattgtggtgctgactaatataatctccccaacctactttgacttctggggccagggaacccgggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [309] g74>t|c108>t|g162>t|g176>c,G59>A(- + -)|c230>g,T77>S(+ - +)|a253>g,S85>D(- - -)|g254>a,S85>D(- - -)| g74>t| c108>t| g162>t| g176>c,G59>A(- + -)| c230>g,T77>S(+ - +)|a253>g,S85>D(- - -)|g254>a,S85>D(- - -)| a316>c|a318>g|a320>t| 315 (320) 220 (225) 213 (215) 7 (10) 3 (6) 4 1 (2) 1 1 0 0 (1) 0 0 (1) 0 1 1 2 0 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 36 23 22 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 30 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 117 114 111 3 0 3 1 1 0 0 0 0 0 0 0 1 0 0 3 (8) 3 3 (5) 0 (3) 0 (3) 0 0 (1) 0 0 0 0 (1) 0 0 (1) 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| tttct ggatacagtttaccagttactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggtatc atctatcctgctgactctgatacc agatacagcccgtccttccaaggccaggtcagcatctcagccgacaagtccatcgacaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatgtattactgt gcgcggctgccatatattgtggtgctgactaatataatctccccaacctactttgacttc ARLPYIVVLTNIISPTYFDF IGHV5-51 IGHD2-21 IGHJ4 ggatacagtttaccagttactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggtatc atctatcctgctgactctgatacc agatacagcccgtccttccaaggccaggtcagcatctcagccgacaagtccatcgacaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatgtattactgt gcgcggctgccatatattgtggtgctgactaatataatctccccaacctactttgacttc IGG1 6 212 3 3 2 5 1 1 1 1 3 1 JY8QFUQ01AKQ1X 17984 productive Homsap IGHV3-48*01 F, or Homsap IGHV3-48*02 F 999 95.85 208/217 nt NA Homsap IGHJ6*02 F 256 90.32 56/62 nt Homsap IGHD4-23*01 ORF 2 8 8 20 8.8.20 [1.17.38.11] CVRGDYGGAPGHYYDPYGLDVW in-frame + ctctggattcaccttcaggagttatatcatgaactgggtccgccaggctccagggaaggggctggagtggatttcatacattagtagtagtggtattatcatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgtgtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtctggggccaagggaccacggtcaaagtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcca NA IGG2 100 100 [309] t105>g,S35>R(- - -)|c108>t|g113>t,S38>I(- - -)|g157>a,V53>I(+ - +)|a184>g,S62>G(+ + -)|g188>t,S63>I(- - -)|c191>t,T64>I(- - -)|c287>t,A96>V(+ - +)|c309>t|c314>t,A105>V(+ - +)| t105>g,S35>R(- - -)|c108>t|g113>t,S38>I(- - -)| g157>a,V53>I(+ - +)| a184>g,S62>G(+ + -)|g188>t,S63>I(- - -)|c191>t,T64>I(- - -)| c287>t,A96>V(+ - +)|c309>t| c314>t,A105>V(+ - +)|a320>g| 319 (320) 224 (225) 214 10 (11) 2 (3) 8 1 (2) 1 5 0 0 0 0 0 0 0 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 1 0 0 0 0 0 0 0 1 1 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 1 0 0 0 0 0 0 0 1 0 117 114 112 2 1 1 0 0 2 0 0 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcaggagttatatc atgaactgggtccgccaggctccagggaaggggctggagtggatttcatac attagtagtagtggtattatcata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgt gtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtc VRGDYGGAPGHYYDPYGLDV IGHV3-48 IGHD4-23 IGHJ6 ggattcaccttcaggagttatatc atgaactgggtccgccaggctccagggaaggggctggagtggatttcatac attagtagtagtggtattatcata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgt gtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtc IGG2 9 213 6 3 5 7 1 2 1 1 2 5 JY8QFUQ01AKROJ 17717 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F (see comment) 729 82.03 178/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-16*01 F 2 8 8 9 8.8.9 [1.17.38.11] CARGGTDFDYW in-frame + Low V-REGION identity (82,03% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctggatttacatttagtgattataatatgaattgggtccgccgggctccagggaaggggctggaatatgtcgcaaccattacaactgggagtggtcaaatctactacagtgactcagtgaggggccgcttcaccatctctagagacgacgccaagaattcactttatctgcagatgaacaacctgagaggcgaggacacggctgtctatttttgtgcgagaggggggactgattttgactattggggccagggaaccctggtcaccgtctcctgcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccccag NA IGA1 100 100 [276] c84>t|c87>a|c90>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|g113>a,S38>N(- - -)|c114>t,S38>N(- - -)|c120>t|a131>g,Q44>R(+ - -)|g153>a|g155>a,W52>Y(- + -)|g156>t,W52>Y(- + -)|t160>g,S54>A(- + -)|t163>a,S55>T(+ - +)|g170>c,S57>T(+ - +)|t171>a,S57>T(+ - +)|g173>c,S58>T(+ - +)|a175>g,S59>G(+ + -)|t177>g,S59>G(+ + -)|a187>g,S63>G(+ + -)|t190>c,Y64>Q(- - -)|c192>a,Y64>Q(- - -)|a195>c|g202>a,A68>S(- + -)|c203>g,A68>S(- + -)|a204>t,A68>S(- + -)|a215>g,K72>R(+ + +)|a225>c|c237>t|a244>g,N82>D(+ + -)|c255>t|g261>t|a270>g|g278>a,S93>N(- - -)|c287>g,A96>G(- + -)|g303>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| c84>t|c87>a|c90>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|g113>a,S38>N(- - -)|c114>t,S38>N(- - -)| c120>t|a131>g,Q44>R(+ - -)|g153>a|g155>a,W52>Y(- + -)|g156>t,W52>Y(- + -)|t160>g,S54>A(- + -)|t163>a,S55>T(+ - +)| g170>c,S57>T(+ - +)|t171>a,S57>T(+ - +)|g173>c,S58>T(+ - +)|a175>g,S59>G(+ + -)|t177>g,S59>G(+ + -)|a187>g,S63>G(+ + -)|t190>c,Y64>Q(- - -)|c192>a,Y64>Q(- - -)|a195>c| g202>a,A68>S(- + -)|c203>g,A68>S(- + -)|a204>t,A68>S(- + -)|a215>g,K72>R(+ + +)|a225>c|c237>t|a244>g,N82>D(+ + -)|c255>t|g261>t|a270>g|g278>a,S93>N(- - -)|c287>g,A96>G(- + -)|g303>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| a320>g| 319 (320) 224 (225) 185 39 (40) 12 (13) 27 7 (8) 6 8 1 2 2 2 2 3 2 2 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 16 8 3 5 1 2 4 0 0 1 0 0 0 0 0 0 51 51 44 7 2 5 1 2 1 0 0 0 0 1 0 0 1 1 30 24 15 9 1 8 2 0 0 1 1 1 0 1 2 0 0 1 117 114 99 15 6 9 3 2 3 0 1 0 2 0 1 2 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatttacatttagtgattataat atgaattgggtccgccgggctccagggaaggggctggaatatgtcgcaacc attacaactgggagtggtcaaatc tactacagtgactcagtgaggggccgcttcaccatctctagagacgacgccaagaattcactttatctgcagatgaacaacctgagaggcgaggacacggctgtctatttttgt gcgagaggggggactgattttgactat ARGGTDFDY IGHV3-21 IGHD3-16 IGHJ4 ggatttacatttagtgattataat atgaattgggtccgccgggctccagggaaggggctggaatatgtcgcaacc attacaactgggagtggtcaaatc tactacagtgactcagtgaggggccgcttcaccatctctagagacgacgccaagaattcactttatctgcagatgaacaacctgagaggcgaggacacggctgtctatttttgt gcgagaggggggactgattttgactat IGA1 39 213 22 17 14 23 8 16 8 4 14 13 JY8QFUQ01AKUKJ 8749 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 957 92.38 206/223 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD5-24*01 ORF 1 8 10 10 8.10.10 [1.17.38.11] CTHLREEMSYEW in-frame + ctctggagtcaaattcagaaacgcctggatgaattgggtccgccaggctccagggaaggggctggagtgggttggccgtattaagagcaaagctgatggtgggacaacagactacgccacacccgtgagaggcagattcaccatctcaagagatgattcaaaaaacacgttttatctgcaaatgaatagcctaaaaaccgaagacacagccgtctattactgtacccacttgcgagaagagatgtcttacgaatggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [284] t82>g,F28>V(+ - -)|c86>a,T29>K(- - -)|t87>a,T29>K(- - -)|t105>a,S35>R(- - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a171>g|a178>g,T60>A(- - -)|t204>c|g205>a,A69>T(- - -)|a215>g,K72>R(+ + +)|c259>t,L87>F(+ - -)|g261>t,L87>F(+ - -)|c276>t|g282>a|g291>a|g303>c| t82>g,F28>V(+ - -)|c86>a,T29>K(- - -)|t87>a,T29>K(- - -)|t105>a,S35>R(- - -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)| a171>g|a178>g,T60>A(- - -)| t204>c|g205>a,A69>T(- - -)|a215>g,K72>R(+ + +)|c259>t,L87>F(+ - -)|g261>t,L87>F(+ - -)|c276>t|g282>a|g291>a|g303>c| a316>c,T106>H(+ - -)|c317>a,T106>H(+ - -)|a318>c,T106>H(+ - -)|g319>t|a320>t| 315 (320) 226 (231) 209 17 (22) 6 (8) 11 (14) 3 4 3 1 0 (2) 1 (2) 0 (1) 2 1 0 1 (2) 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 0 0 1 0 2 0 0 0 1 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 30 28 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 117 114 105 9 5 4 1 3 2 1 0 0 0 0 1 0 1 0 3 (8) 3 3 0 (5) 0 (2) 0 (3) 0 0 0 0 0 (2) 0 (1) 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| ctct ggagtcaaattcagaaacgcctgg atgaattgggtccgccaggctccagggaaggggctggagtgggttggccgt attaagagcaaagctgatggtgggacaaca gactacgccacacccgtgagaggcagattcaccatctcaagagatgattcaaaaaacacgttttatctgcaaatgaatagcctaaaaaccgaagacacagccgtctattactgt acccacttgcgagaagagatgtcttacgaa THLREEMSYE IGHV3-15 IGHD5-24 IGHJ4 ggagtcaaattcagaaacgcctgg atgaattgggtccgccaggctccagggaaggggctggagtgggttggccgt attaagagcaaagctgatggtgggacaaca gactacgccacacccgtgagaggcagattcaccatctcaagagatgattcaaaaaacacgttttatctgcaaatgaatagcctaaaaaccgaagacacagccgtctattactgt acccacttgcgagaagagatgtcttacgaa IGA1 17 219 11 6 7 10 4 7 5 1 6 5 JY8QFUQ01AKVLH 13947 productive (see comment) Homsap IGHV3-23*05 F 831 87.85 188/214 nt 87.38 187/214 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD1-14*01 ORF 1 8 7 10 8.7.10 [1.17.38.11] CAKRPPGTIDRW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 63 of V-REGION: 3 nucleotides (from position 95 in the user submitted sequence), (do not cause frameshift) ctctggattcacctttggcacctctgacatggcctgggtccgccaggttccaggggaggggctggagtgggtctcacacattgatatcagaggtgccacacagtataaagactccgtgaagggccggttcaccatctccagagacaattccaagagcactctatatctgcaaatgaacaccttgcgagccgaggacacggccgtatattactgtgcgaaacgcccgcccggcaccatagatcgctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [275] a103>g,S35>G(+ + -)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|c113>a,A38>D(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g163>c,A55>H(- - -)|c164>a,A55>H(- - -)|t165>c,A55>H(- - -)|t169>g,Y57>D(- - -)|g173>t,S58>I(- - -)|t177>a,S59>R(- - -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|t196>c,Y66>Q(- - -)|c198>g,Y66>Q(- - -)|g202>a,A68>K(- - -)|c203>a,A68>K(- - -)|a254>g,N85>S(- - -)|g258>t|g261>a|g278>c,S93>T(+ - +)|c280>t|a283>c| a103>g,S35>G(+ + -)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|c113>a,A38>D(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g163>c,A55>H(- - -)|c164>a,A55>H(- - -)|t165>c,A55>H(- - -)| t169>g,Y57>D(- - -)|g173>t,S58>I(- - -)|t177>a,S59>R(- - -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| t196>c,Y66>Q(- - -)|c198>g,Y66>Q(- - -)|g202>a,A68>K(- - -)|c203>a,A68>K(- - -)|a254>g,N85>S(- - -)|g258>t|g261>a|g278>c,S93>T(+ - +)|c280>t|a283>c| 318 220 194 26 4 22 5 2 2 2 2 3 0 1 5 1 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 0 0 0 1 1 0 0 1 0 0 0 51 51 44 7 0 7 2 0 1 1 0 1 0 0 2 0 0 0 30 21 16 5 0 5 1 0 0 0 0 0 0 1 1 0 1 1 117 114 104 10 4 6 1 2 1 1 1 1 0 0 1 1 1 0 6 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|agca,172-175(CDR2)|ggta,184-187(CDR2)|agca,190-193(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttggcacctctgac atggcctgggtccgccaggttccaggggaggggctggagtgggtctcacac attgatatcagaggtgccaca cagtataaagactccgtgaagggccggttcaccatctccagagacaattccaagagcactctatatctgcaaatgaacaccttgcgagccgaggacacggccgtatattactgt gcgaaacgcccgcccggcaccatagatcgc AKRPPGTIDR IGHV3-23 IGHD1-14 IGHJ4 ggattcacctttggcacctctgac atggcctgggtccgccaggttccaggggaggggctggagtgggtctcacac attgatatcagaggtgccaca cagtataaagactccgtgaagggccggttcaccatctccagagacaattccaagagcactctatatctgcaaatgaacaccttgcgagccgaggacacggccgtatattactgt gcgaaacgcccgcccggcaccatagatcgc IGA1 26 210 11 15 4 15 7 11 4 0 13 9 JY8QFUQ01AKY8Y 7166 productive (see comment) Homsap IGHV3-48*01 F (see comment) 744 83.89 177/211 nt 82.94 175/211 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-12*01 F 3 8 7 12 8.7.12 [0.17.38.11] CSRDGRRGYDLDSW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (83,89% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR2-IMGT at codon 52 of V-REGION: 'G' (1 nucleotides at position 66 to 66 in the user submitted sequence), (cause frameshift); in CDR2-IMGT, from codon 57 of V-REGION: 3 nucleotides (from position 80 in the user submitted sequence), (do not cause frameshift) tggattcaccttcggaacctatgccatgacgtgggtccgcctgactcctgggaaagggctggagtGgggtttcatggattagtgatatcggtgacacacgctatgcagattctgtgaagggccgattcaccatctccagagacaatgccaagaattcactgtttctgcaaatggacagtctcagagccgacgacacggctatatattattgttcgagagatggccggaggggctacgaccttgactcctggggccagggagccctggtcaccgtctcctcatcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [279, 278] a103>g,S35>G(+ + -)|t105>a,S35>G(+ + -)|g107>c,S36>T(+ - +)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)|a119>c,N40>T(- + -)|c120>g,N40>T(- + -)|a131>t,Q44>L(- - -)|g133>a,A45>T(- - -)|a138>t|g144>a|a164>g,Y55>W(- + -)|c165>g,Y55>W(- + -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g185>t,S62>I(- - -)|t186>c,S62>I(- - -)|a187>g,S63>G(+ + -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|t194>c,I65>T(- - -)|t196>c,Y66>R(- - -)|a197>g,Y66>R(- - -)|c201>t|c207>t|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|g282>c|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t|g313>t,A105>S(- + -)| a103>g,S35>G(+ + -)|t105>a,S35>G(+ + -)|g107>c,S36>T(+ - +)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)| a119>c,N40>T(- + -)|c120>g,N40>T(- + -)|a131>t,Q44>L(- - -)|g133>a,A45>T(- - -)|a138>t|g144>a|a164>g,Y55>W(- + -)|c165>g,Y55>W(- + -)| a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g185>t,S62>I(- - -)|t186>c,S62>I(- - -)|a187>g,S63>G(+ + -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|t194>c,I65>T(- - -)| t196>c,Y66>R(- - -)|a197>g,Y66>R(- - -)|c201>t|c207>t|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|g282>c|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| g313>t,A105>S(- + -)| 320 219 184 35 8 27 8 5 5 3 1 1 3 1 4 2 2 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 2 0 0 0 0 0 0 1 2 0 0 0 51 51 43 8 2 6 1 2 0 0 1 0 2 0 0 2 0 0 30 21 13 8 0 8 3 1 0 2 0 1 0 0 0 0 1 0 117 114 101 13 6 7 2 2 5 1 0 0 1 0 2 0 0 0 8 8 7 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t ggattcaccttcggaacctatgcc atgacgtgggtccgcctgactcctgggaaagggctggagtgggtttcatgg attagtgatatcggtgacaca cgctatgcagattctgtgaagggccgattcaccatctccagagacaatgccaagaattcactgtttctgcaaatggacagtctcagagccgacgacacggctatatattattgt tcgagagatggccggaggggctacgaccttgactcc SRDGRRGYDLDS IGHV3-48 IGHD5-12 IGHJ4 ggattcaccttcggaacctatgcc atgacgtgggtccgcctgactcctgggaaagggctggagtgggtttcatgg attagtgatatcggtgacaca cgctatgcagattctgtgaagggccgattcaccatctccagagacaatgccaagaattcactgtttctgcaaatggacagtctcagagccgacgacacggctatatattattgt tcgagagatggccggaggggctacgaccttgactcc IGA2 34 210 21 13 10 18 11 16 8 0 13 13 JY8QFUQ01AKZ0I 10436 productive (see comment) Homsap IGHV4-39*03 F 937 92.2 201/218 nt 91.28 199/218 nt Homsap IGHJ3*01 F 232 96 48/50 nt Homsap IGHD3-22*01 F 3 9 6 17 9.6.17 [1.17.38.11] CARSITLTVVVMNDAFDVW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 35 of V-REGION: 1 nucleotides (from position 26 in the user submitted sequence), (cause frameshift)in CDR2-IMGT, from codon 64 of V-REGION: 1 nucleotides (from position 102 in the user submitted sequence), (cause frameshift) tctccggtggctccatcaacagtagaattattattggggctggatccgccagcccccagggaagggtttggagtggattggaaatatctattatagtgggacacctactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgtgcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtctggggccaagggacattggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [302] t78>c|g92>a,S31>N(- - -)|g107>a,S36>N(- - -)|c111>t|c114>t|g147>t|c148>t|g162>a|g164>a,S55>N(- - -)|c204>t|a232>g,I78>V(+ - +)|c245>g,T82>R(- - -)|c249>t|c261>g,F87>L(+ - -)|g275>c,S92>T(+ - +)|g280>c,V94>L(+ - +)|g303>a| t78>c| g92>a,S31>N(- - -)|g107>a,S36>N(- - -)|c111>t|c114>t| g147>t|c148>t|g162>a|g164>a,S55>N(- - -)| c204>t|a232>g,I78>V(+ - +)|c245>g,T82>R(- - -)|c249>t|c261>g,F87>L(+ - -)|g275>c,S92>T(+ - +)|g280>c,V94>L(+ - +)|g303>a| 311 218 201 17 9 8 1 5 5 1 0 0 0 0 2 2 1 0 78 5 4 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 29 25 4 2 2 0 2 2 0 0 0 0 0 0 0 0 0 51 51 47 4 3 1 0 2 1 0 0 0 0 0 0 0 1 0 30 20 20 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 116 114 105 8 3 5 1 1 2 0 0 0 0 0 2 2 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctcc ggtggctccatcaacagtagaattattat tggggctggatccgccagcccccagggaagggtttggagtggattggaaat atctattatagtgggacacc tactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgt gcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtc ARSITLTVVVMNDAFDV IGHV4-39 IGHD3-22 IGHJ3 ggtggctccatcaacagtagaattattat tggggctggatccgccagcccccagggaagggtttggagtggattggaaat atctattatagtgggacacc tactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgt gcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtc IGG1 16 214 11 5 10 15 1 1 6 2 6 2 JY8QFUQ01AKZW4 20877 productive (see comment) Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F 957 94.39 202/214 nt 93.93 201/214 nt Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 201 88.24 45/51 nt Homsap IGHD1-26*01 F 1 8 7 18 8.7.18 [1.17.38.11] CARGWAQQAGATFVGWFDSW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*03 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 48 to 48 in the user submitted sequence), (cause frameshift); ctatggtgggtccttcagtggttacacctggaactggatccgccagcCccccagggaaggggctggagtggattggggaaatcaatcatagtggcaggaccaactacaacccgtccctcaagagtcgagtcaccatatcaatagacacgaccaacaaccagttctccctgaagttgacctctgtgaccgccgcggacacggctgtatatttctgtgcgagaggttgggcccaacaagcgggagctacgtttgttggctggttcgactcctggggccaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgctgcctggtccagggcttcttccccca NA IGA1 79 89 [300] t112>a,Y38>T(+ - -)|a113>c,Y38>T(+ - -)|g119>a,S40>N(- - -)|a189>c|c192>g,S64>R(- - -)|g238>a,V80>I(+ - +)|t247>a,S83>T(+ - +)|g252>c,K84>N(+ - -)|c271>t|g275>c,S92>T(+ - +)|g303>a|a308>t,Y103>F(- + -)| t112>a,Y38>T(+ - -)|a113>c,Y38>T(+ - -)| g119>a,S40>N(- - -)| a189>c|c192>g,S64>R(- - -)| g238>a,V80>I(+ - +)|t247>a,S83>T(+ - +)|g252>c,K84>N(+ - -)|c271>t|g275>c,S92>T(+ - +)|g303>a|a308>t,Y103>F(- + -)| 320 222 210 12 3 9 0 3 1 0 2 0 1 2 2 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 1 0 0 1 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 21 19 2 1 1 0 0 0 0 1 0 0 0 0 1 0 0 117 114 107 7 2 5 0 2 1 0 0 0 1 1 2 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctat ggtgggtccttcagtggttacacc tggaactggatccgccagcccccagggaaggggctggagtggattggggaa atcaatcatagtggcaggacc aactacaacccgtccctcaagagtcgagtcaccatatcaatagacacgaccaacaaccagttctccctgaagttgacctctgtgaccgccgcggacacggctgtatatttctgt gcgagaggttgggcccaacaagcgggagctacgtttgttggctggttcgactcc ARGWAQQAGATFVGWFDS IGHV4-34 IGHD1-26 IGHJ5 ggtgggtccttcagtggttacacc tggaactggatccgccagcccccagggaaggggctggagtggattggggaa atcaatcatagtggcaggacc aactacaacccgtccctcaagagtcgagtcaccatatcaatagacacgaccaacaaccagttctccctgaagttgacctctgtgaccgccgcggacacggctgtatatttctgt gcgagaggttgggcccaacaagcgggagctacgtttgttggctggttcgactcc IGA1 12 210 4 8 4 7 0 5 2 1 6 3 JY8QFUQ01AL1GZ 6919 productive Homsap IGHV3-73*01 F, or Homsap IGHV3-73*02 F 1007 94.64 212/224 nt NA Homsap IGHJ6*01 F (see comment) 180 76.19 48/63 nt Homsap IGHD4-11*01 ORF 3 8 10 18 8.10.18 [1.17.38.11] CVRHEGTPVSDVGGYGMDVW in-frame + Other possibilities: Homsap_IGHJ4*01 (shorter alignment but highest percentage of identity) cctctggattcaccttcagtggctctgctatgcactgggtccgccaggtttccgggaaagggctggagtgggttggccgtattagaagcaaagcttacaattccgcgacagcatatgctgcgtcggtgaaaggcaggttcaccatctccagagatgattcaaagaacacggcgtatttggaaatgaacagtttgaagagggaggacacggccgtgtattactgtgtgagacatgaagggactccagtaagtgatgtaggtggatacggcatggacgtctggggtcaagggtccacggtcaccgtctcctcagcatccccgaccagccccaaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 89 [310, 309] g81>a|c134>t,A45>V(+ - +)|a181>t,N61>Y(- - -)|g185>a,S62>N(- - -)|a188>c,Y63>S(+ - -)|c265>t|c268>g,Q90>E(+ + -)|c279>t|c280>t|a285>g|c287>g,T96>R(- - -)|c288>g,T96>R(- - -)| g81>a| c134>t,A45>V(+ - +)| a181>t,N61>Y(- - -)|g185>a,S62>N(- - -)|a188>c,Y63>S(+ - -)| c265>t|c268>g,Q90>E(+ + -)|c279>t|c280>t|a285>g|c287>g,T96>R(- - -)|c288>g,T96>R(- - -)| a313>g,T105>V(- - -)|c314>t,T105>V(- - -)|t315>g,T105>V(- - -)| 320 232 217 15 5 10 2 2 5 0 1 0 1 0 0 3 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 30 27 3 0 3 0 1 0 0 1 0 1 0 0 0 0 0 117 114 107 7 4 3 1 0 3 0 0 0 0 0 0 3 0 0 8 8 5 3 0 3 1 0 1 0 0 0 0 0 0 0 0 1 aa,58-59(FR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,114-115(CDR1)|tt,135-136(FR2)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|ggct,106-109(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|agca,195-198(CDR2)|ggca,220-223(FR3)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,59-62(FR1)|agcc,72-75(FR1)|tgct,111-114(CDR1)|agct,177-180(CDR2)|tgct,201-204(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| cctct ggattcaccttcagtggctctgct atgcactgggtccgccaggtttccgggaaagggctggagtgggttggccgt attagaagcaaagcttacaattccgcgaca gcatatgctgcgtcggtgaaaggcaggttcaccatctccagagatgattcaaagaacacggcgtatttggaaatgaacagtttgaagagggaggacacggccgtgtattactgt gtgagacatgaagggactccagtaagtgatgtaggtggatacggcatggacgtc VRHEGTPVSDVGGYGMDV IGHV3-73 IGHD4-11 IGHJ6 ggattcaccttcagtggctctgct atgcactgggtccgccaggtttccgggaaagggctggagtgggttggccgt attagaagcaaagcttacaattccgcgaca gcatatgctgcgtcggtgaaaggcaggttcaccatctccagagatgattcaaagaacacggcgtatttggaaatgaacagtttgaagagggaggacacggccgtgtattactgt gtgagacatgaagggactccagtaagtgatgtaggtggatacggcatggacgtc IGA1 12 219 7 5 6 9 1 3 4 1 4 3 JY8QFUQ01AL1N5 11276 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 878 89.45 195/218 nt NA Homsap IGHJ3*01 F 178 84 42/50 nt Homsap IGHD6-19*01 F 1 8 8 16 8.8.16 [1.17.38.11] CAKEQRGSGWSRSALDVW in-frame + cctctggattcacgtttggcagccacgccatgagctgggtccgccaggctccagggaaggggctggagtacgtctcaattgttactggtagcggacgcagcacatactacgcagagtctgtgaagggccggttcaccgtctccagagacaattccaaggacaccctgtatctgcaaatggacagcctgagacccgaggacacggccgtgtattattgtgcgaaagaacaacgaggcagtggctggtcccgatcggctcttgatgtctggggccaaggggcatgggtcaccgtctcttcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggctgacggtgtcgtggaactca NA IGG2 100 100 [298] c87>g|a103>g,S35>G(+ + -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|g163>a,A55>I(+ - +)|c164>t,A55>I(+ - +)|a166>g,I56>V(+ - +)|g170>c,S57>T(+ - +)|t177>c|t186>a|g187>c,G63>R(- - -)|t189>c,G63>R(- - -)|c207>g,D69>E(+ - +)|c210>t|a232>g,I78>V(+ - +)|a253>g,N85>D(+ + -)|g258>c|a274>g,N92>D(+ + -)|g286>c,A96>P(- - -)|a303>g|c309>t| c87>g|a103>g,S35>G(+ + -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)| g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|g163>a,A55>I(+ - +)|c164>t,A55>I(+ - +)| a166>g,I56>V(+ - +)|g170>c,S57>T(+ - +)|t177>c|t186>a|g187>c,G63>R(- - -)|t189>c,G63>R(- - -)| c207>g,D69>E(+ - +)|c210>t|a232>g,I78>V(+ - +)|a253>g,N85>D(+ + -)|g258>c|a274>g,N92>D(+ + -)|g286>c,A96>P(- - -)|a303>g|c309>t| 320 226 203 23 7 16 6 2 3 4 0 0 0 1 5 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 0 2 0 0 0 0 0 1 0 0 51 51 47 4 0 4 0 2 1 0 0 0 0 0 1 0 0 0 30 24 18 6 2 4 1 0 0 2 0 0 0 1 2 0 0 0 117 114 105 9 4 5 4 0 2 0 0 0 0 0 2 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacgtttggcagccacgcc atgagctgggtccgccaggctccagggaaggggctggagtacgtctcaatt gttactggtagcggacgcagcaca tactacgcagagtctgtgaagggccggttcaccgtctccagagacaattccaaggacaccctgtatctgcaaatggacagcctgagacccgaggacacggccgtgtattattgt gcgaaagaacaacgaggcagtggctggtcccgatcggctcttgatgtc AKEQRGSGWSRSALDV IGHV3-23 IGHD6-19 IGHJ3 ggattcacgtttggcagccacgcc atgagctgggtccgccaggctccagggaaggggctggagtacgtctcaatt gttactggtagcggacgcagcaca tactacgcagagtctgtgaagggccggttcaccgtctccagagacaattccaaggacaccctgtatctgcaaatggacagcctgagacccgaggacacggccgtgtattattgt gcgaaagaacaacgaggcagtggctggtcccgatcggctcttgatgtc IGG2 23 213 15 8 5 12 10 11 4 3 9 7 JY8QFUQ01AL2CW 17859 productive (see comment) Homsap IGHV3-49*03 F, or Homsap IGHV3-49*05 F 1115 100 224/224 nt 99.55 223/224 nt Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD4-17*01 F 3 8 10 12 8.10.12 [1.17.38.11] CTKCSVTIYFFDYW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 72 of V-REGION: 'A' (1 nucleotides at position 129 to 129 in the user submitted sequence), (cause frameshift); cttctggattcacctttggtgattatgctatgagctggttccgccaggctccagggaaggggctggagtgggtaggtttcattagaagcaaagcttatggtgggacaacagaatacgccgcgtctgtgAaaaggcagattcaccatctcaagagatgattccaaaagcatcgcctatctgcaaatgaacagcctgaaaaccgaggacacagccgtgtattactgtactaagtgttcggtgactatttacttttttgactactggggccagggaaccctggtcaccgtctcctcagcttccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG3 100 100 [293] g317>a,R106>K(+ + +)|a318>g,R106>K(+ + +)|g319>t|a320>g| 316 (320) 228 (232) 228 0 (4) 0 (2) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 0 0 0 (1) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,158-159(FR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,181-182(CDR2)|aa,192-193(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|ta,69-70(FR1)|tt,75-76(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,124-125(FR2)|ta,158-159(FR2)|tt,162-163(FR2)|tt,167-168(CDR2)|tt,180-181(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agct,72-75(FR1)|agct,118-121(FR2)|ggtt,122-125(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggta,156-159(FR2)|ggtt,160-163(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|ggca,220-223(FR3)|agca,253-256(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,72-75(FR1)|tgct,111-114(CDR1)|agct,118-121(FR2)|agct,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| cttct ggattcacctttggtgattatgct atgagctggttccgccaggctccagggaaggggctggagtgggtaggtttc attagaagcaaagcttatggtgggacaaca gaatacgccgcgtctgtgaaaggcagattcaccatctcaagagatgattccaaaagcatcgcctatctgcaaatgaacagcctgaaaaccgaggacacagccgtgtattactgt actaagtgttcggtgactatttacttttttgactac TKCSVTIYFFDY IGHV3-49 IGHD4-17 IGHJ4 ggattcacctttggtgattatgct atgagctggttccgccaggctccagggaaggggctggagtgggtaggtttc attagaagcaaagcttatggtgggacaaca gaatacgccgcgtctgtgaaaggcagattcaccatctcaagagatgattccaaaagcatcgcctatctgcaaatgaacagcctgaaaaccgaggacacagccgtgtattactgt actaagtgttcggtgactatttacttttttgactac IGG3 0 219 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01AL2IL 20821 productive Homsap IGHV3-7*01 F 887 89.91 196/218 nt NA Homsap IGHJ6*02 F 265 91.94 57/62 nt Homsap IGHD3-22*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARDPSYSYYNGLDVW in-frame + cctctaaattcacttttagtaactattggatgaattgggtccgccaggctccagcgaagggactggagtgggtggccagtataaagcaggatgggggggagacatattatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaagtcactgtatctgcaaatgaacagcctgggagtcgaagacacggctgtttattactgtgcgagagatccatcttactcctactacaatggtttggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [291] g79>a,G27>K(- - -)|g80>a,G27>K(- - -)|c87>t|g107>a,S36>N(- - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g140>c,G47>A(- + -)|g147>a|a164>g,N55>S(- - -)|c165>t,N55>S(- - -)|a174>g|a186>g|a187>g,S63>G(+ + -)|t189>g,S63>G(+ + -)|a194>c,K65>T(- - -)|c198>t|t203>c,V68>A(+ - +)|c255>g,N85>K(+ - -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g291>a|g303>t| g79>a,G27>K(- - -)|g80>a,G27>K(- - -)|c87>t|g107>a,S36>N(- - -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g140>c,G47>A(- + -)|g147>a|a164>g,N55>S(- - -)|c165>t,N55>S(- - -)| a174>g|a186>g|a187>g,S63>G(+ + -)|t189>g,S63>G(+ + -)|a194>c,K65>T(- - -)| c198>t|t203>c,V68>A(+ - +)|c255>g,N85>K(+ - -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g291>a|g303>t| 320 226 204 22 7 15 5 6 5 1 1 0 0 0 1 1 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 3 1 0 0 0 0 0 0 0 0 0 51 51 45 6 1 5 1 2 2 0 0 0 0 0 1 0 0 0 30 24 19 5 2 3 3 0 0 0 1 0 0 0 0 0 0 1 117 114 107 7 3 4 1 1 2 1 0 0 0 0 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct aaattcacttttagtaactattgg atgaattgggtccgccaggctccagcgaagggactggagtgggtggccagt ataaagcaggatgggggggagaca tattatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaagtcactgtatctgcaaatgaacagcctgggagtcgaagacacggctgtttattactgt gcgagagatccatcttactcctactacaatggtttggacgtc ARDPSYSYYNGLDV IGHV3-7 IGHD3-22 IGHJ6 aaattcacttttagtaactattgg atgaattgggtccgccaggctccagcgaagggactggagtgggtggccagt ataaagcaggatgggggggagaca tattatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaagtcactgtatctgcaaatgaacagcctgggagtcgaagacacggctgtttattactgt gcgagagatccatcttactcctactacaatggtttggacgtc IGA1 22 213 17 5 11 14 6 8 4 3 9 6 JY8QFUQ01AL7F1 18806 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 842 87.61 191/218 nt NA Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD6-19*01 F 1 8 8 17 8.8.17 [1.17.38.11] CARDSASSGYARYDAMDVW in-frame + cctctggattcagttttagtacacatggcatgaactgggtccgccaggctccagggaaggggccggaatgggtctcattcgttaatagtggaagtagttacatctactacgcagactcagtgaggggccgattcaccatctccagagacgacgccaggaattcactgtatctgcaaatgcaccgcctgcgagtcgaggacacggctctctactattgtgcgagagacagtgcctcgtccggctacgctaggtacgacgctatggacgtctggggccaagggaccgcggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggctgtcgtggaactc NA IGG2 100 100 [301] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>t|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|t109>c,Y37>H(+ - -)|a112>g,S38>G(+ + -)|t149>c,L50>P(- - -)|g153>a|c164>t,S55>F(- - -)|a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|a195>c|a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|a251>g,K84>R(+ + +)|c255>t|a274>c,N92>H(- - -)|a277>c,S93>R(- - -)|a283>c|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t306>c|c309>t| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>t|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|t109>c,Y37>H(+ - -)|a112>g,S38>G(+ + -)| t149>c,L50>P(- - -)|g153>a|c164>t,S55>F(- - -)| a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|a195>c| a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|a251>g,K84>R(+ + +)|c255>t|a274>c,N92>H(- - -)|a277>c,S93>R(- - -)|a283>c|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t306>c|c309>t| 320 226 199 27 7 20 6 2 6 3 4 1 0 1 3 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 1 6 1 0 2 1 0 1 0 0 1 1 0 0 51 51 48 3 1 2 0 1 1 1 0 0 0 0 0 0 0 0 30 24 19 5 1 4 2 1 0 0 1 0 0 1 0 0 0 0 117 114 102 12 4 8 3 0 3 1 3 0 0 0 2 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcagttttagtacacatggc atgaactgggtccgccaggctccagggaaggggccggaatgggtctcattc gttaatagtggaagtagttacatc tactacgcagactcagtgaggggccgattcaccatctccagagacgacgccaggaattcactgtatctgcaaatgcaccgcctgcgagtcgaggacacggctctctactattgt gcgagagacagtgcctcgtccggctacgctaggtacgacgctatggacgtc ARDSASSGYARYDAMDV IGHV3-21 IGHD6-19 IGHJ6 ggattcagttttagtacacatggc atgaactgggtccgccaggctccagggaaggggccggaatgggtctcattc gttaatagtggaagtagttacatc tactacgcagactcagtgaggggccgattcaccatctccagagacgacgccaggaattcactgtatctgcaaatgcaccgcctgcgagtcgaggacacggctctctactattgt gcgagagacagtgcctcgtccggctacgctaggtacgacgctatggacgtc IGG2 27 213 17 10 8 13 9 14 5 2 10 10 JY8QFUQ01AL8VS 18022 productive (see comment) Homsap IGHV3-73*01 F, or Homsap IGHV3-73*02 F 800 86.05 185/215 nt 85.58 184/215 nt Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD3-22*01 F 2 8 7 18 8.7.18 [1.17.38.11] CARADYYDTSAYYLDAFDIW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 60 of V-REGION: 9 nucleotides (from position 93 in the user submitted sequence), (do not cause frameshift) ctctggggtacagcttgcgaggctctgttttgcactgggtccgccaggcttccgggaaaggactggagtgggttggccgtgtcggaaggcagggcgcgacaacatacgctgcgtcggtgaaaggcaggttcctcatctccagagatgatccagcgaacacggcttatctggaaatgaacagcctgaaaaccgaggacacggccgtctattactgtgctcgcgcagattactatgatactagtgcttattatctcgatgcttttgatatctggggccaggggacaattgtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [300] c75>t|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)|t83>a,F28>Y(- + -)|c86>g,T29>S(+ - +)|c90>g,F30>L(+ - -)|a103>c,S35>R(- - -)|t105>a,S35>R(- - -)|c113>t,A38>V(+ - +)|a115>t,M39>L(+ + -)|g147>a|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a169>g,R57>G(- - -)|c174>g,S58>R(- - -)|a175>c,K59>Q(+ - -)|a177>g,K59>Q(+ - -)|t187>g,Y63>G(+ - -)|a188>g,Y63>G(+ - -)|g196>a,A66>T(- - -)|t201>c|a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|t247>c,S83>P(+ - -)|a250>g,K84>A(- - -)|a251>c,K84>A(- - -)|g261>t|c268>g,Q90>E(+ + -)|g303>c|a313>g,T105>A(- - -)| c75>t|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)| t83>a,F28>Y(- + -)|c86>g,T29>S(+ - +)|c90>g,F30>L(+ - -)|a103>c,S35>R(- - -)|t105>a,S35>R(- - -)|c113>t,A38>V(+ - +)| a115>t,M39>L(+ + -)|g147>a| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a169>g,R57>G(- - -)|c174>g,S58>R(- - -)|a175>c,K59>Q(+ - -)|a177>g,K59>Q(+ - -)|t187>g,Y63>G(+ - -)|a188>g,Y63>G(+ - -)| g196>a,A66>T(- - -)|t201>c|a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|t247>c,S83>P(+ - -)|a250>g,K84>A(- - -)|a251>c,K84>A(- - -)|g261>t|c268>g,Q90>E(+ + -)|g303>c| a313>g,T105>A(- - -)|a316>c|a318>c|c319>g|a320>c| 315 (320) 218 (223) 187 (188) 31 (35) 5 (9) 26 6 2 4 4 4 (7) 0 1 2 1 4 (5) 1 2 78 5 1 4 1 3 0 0 2 1 0 0 0 0 0 0 0 1 36 24 18 6 0 6 0 0 1 0 1 0 0 2 0 2 0 0 51 51 49 2 1 1 0 1 0 0 0 0 1 0 0 0 0 0 30 21 13 8 0 8 4 0 0 1 1 0 0 0 0 1 0 1 117 114 104 10 3 7 1 1 1 2 2 0 0 0 1 1 1 0 3 (8) 3 2 (3) 1 (5) 0 (4) 1 1 0 0 0 0 (3) 0 0 0 0 0 (1) 0 0 aa,58-59(FR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,114-115(CDR1)|tt,135-136(FR2)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|ggct,106-109(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|agca,195-198(CDR2)|ggca,220-223(FR3)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,59-62(FR1)|agcc,72-75(FR1)|tgct,111-114(CDR1)|agct,177-180(CDR2)|tgct,201-204(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| ctctg gggtacagcttgcgaggctctgtt ttgcactgggtccgccaggcttccgggaaaggactggagtgggttggccgt gtcggaaggcagggcgcgaca acatacgctgcgtcggtgaaaggcaggttcctcatctccagagatgatccagcgaacacggcttatctggaaatgaacagcctgaaaaccgaggacacggccgtctattactgt gctcgcgcagattactatgatactagtgcttattatctcgatgcttttgatatc ARADYYDTSAYYLDAFDI IGHV3-73 IGHD3-22 IGHJ3 gggtacagcttgcgaggctctgtt ttgcactgggtccgccaggcttccgggaaaggactggagtgggttggccgt gtcggaaggcagggcgcgaca acatacgctgcgtcggtgaaaggcaggttcctcatctccagagatgatccagcgaacacggcttatctggaaatgaacagcctgaaaaccgaggacacggccgtctattactgt gctcgcgcagattactatgatactagtgcttattatctcgatgcttttgatatc IGA1 26 210 12 14 4 10 8 16 4 0 8 14 JY8QFUQ01AL9MI 18657 productive Homsap IGHV3-11*01 F (see comment) 788 84.86 185/218 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD6-19*01 F 3 8 8 16 8.8.16 [1.17.38.11] CVRDGARRTNQWLAHDFW in-frame - Low V-REGION identity (84,86% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release cctctggattcagctttaatgccttctatatgggttgggtccgccaggctccaggaaaggggctggagtggatttcgtacattaactctggtggtagtgacacatattacgcagactctgtgaagggccgattcaccatctccagggacaacgcctgggacacaatgtacttggaaatgaacagcctgagagccgaagacacggccgtctactattgtgtgagagatggcgctagaaggacgaatcagtggctggcccatgacttctggggccagggaaccctggtcaccgtctccgcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] c86>g,T29>S(+ - +)|c90>t|g104>a,S35>N(- - -)|a107>c,D36>A(- - -)|a110>t,Y37>F(- + -)|c114>t|a118>g,S40>G(+ + -)|c120>t,S40>G(+ + -)|a124>g,I42>V(+ - +)|g141>a|g157>a,V53>I(+ - +)|a162>g|g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|a172>t|g173>c|a175>g,S59>G(+ + -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|t194>c,I65>T(- - -)|c198>t|a250>t,K84>W(- - -)|a251>g,K84>W(- - -)|a253>g,N85>D(+ + -)|t256>a,S86>T(+ - +)|c259>a,L87>M(+ + -)|t264>c|c265>t|c268>g,Q90>E(+ + -)|g291>a|g303>c|t306>c|c309>t|c314>t,A105>V(+ - +)| c86>g,T29>S(+ - +)|c90>t|g104>a,S35>N(- - -)|a107>c,D36>A(- - -)|a110>t,Y37>F(- + -)|c114>t| a118>g,S40>G(+ + -)|c120>t,S40>G(+ + -)|a124>g,I42>V(+ - +)|g141>a|g157>a,V53>I(+ - +)|a162>g| g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|a172>t|g173>c|a175>g,S59>G(+ + -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|t194>c,I65>T(- - -)| c198>t|a250>t,K84>W(- - -)|a251>g,K84>W(- - -)|a253>g,N85>D(+ + -)|t256>a,S86>T(+ - +)|c259>a,L87>M(+ + -)|t264>c|c265>t|c268>g,Q90>E(+ + -)|g291>a|g303>c|t306>c|c309>t| c314>t,A105>V(+ - +)| 320 226 192 34 13 21 7 5 7 4 1 2 3 1 2 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 2 4 0 1 2 0 1 0 1 0 0 1 0 0 51 51 45 6 2 4 3 2 1 0 0 0 0 0 0 0 0 0 30 24 16 8 2 6 2 1 0 2 0 1 1 0 1 0 0 0 117 114 101 13 7 6 2 1 3 2 0 1 1 1 1 1 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcagctttaatgccttctat atgggttgggtccgccaggctccaggaaaggggctggagtggatttcgtac attaactctggtggtagtgacaca tattacgcagactctgtgaagggccgattcaccatctccagggacaacgcctgggacacaatgtacttggaaatgaacagcctgagagccgaagacacggccgtctactattgt gtgagagatggcgctagaaggacgaatcagtggctggcccatgacttc VRDGARRTNQWLAHDF IGHV3-11 IGHD6-19 IGHJ4 ggattcagctttaatgccttctat atgggttgggtccgccaggctccaggaaaggggctggagtggatttcgtac attaactctggtggtagtgacaca tattacgcagactctgtgaagggccgattcaccatctccagggacaacgcctgggacacaatgtacttggaaatgaacagcctgagagccgaagacacggccgtctactattgt gtgagagatggcgctagaaggacgaatcagtggctggcccatgacttc IGA1 33 213 22 11 11 17 11 16 9 4 10 10 JY8QFUQ01AL9ZT 19861 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 783 84.79 184/217 nt 84.33 183/217 nt Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD3-10*02 F 2 8 8 20 8.8.20 [1.17.38.11] CTKTRVVLGITFPFYIYGLDVW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (84,79% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR3-IMGT at codon 88 of V-REGION: 'T' (1 nucleotides at position 167 to 167 in the user submitted sequence), (cause frameshift); ctctggattcacgttcgacaactatgccatgagctgggtccgccaggcaccaggaaaggggctggagtgggtctccagtattagtggtaatggagaaattgtacaccacgcagacgccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgTtttttgcaaatgaatggagtgagagacgacgacacggccatttactactgtacgaaaacacgcgttgtgttgggaattacatttccgttctatatctatggtctggacgtctggggccaagggaccacggtcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [309] c87>g|t90>c|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)|t135>a|g141>a|a162>c|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|g176>a,S59>N(- - -)|t186>a|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>t,S64>I(- - -)|c192>t,S64>I(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|t196>c,Y66>H(+ - -)|t199>c,Y67>H(+ - -)|t208>g,S70>A(- + -)|t246>c|a263>t,Y88>F(- + -)|c265>t|c276>t|a277>g,S93>G(+ + -)|c279>a,S93>G(+ + -)|c280>g,L94>V(+ - +)|c287>a,A96>D(- - -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)|t306>c|g313>a,A105>T(- - -)| c87>g|t90>c|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)| t135>a|g141>a|a162>c|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| g176>a,S59>N(- - -)|t186>a|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>t,S64>I(- - -)|c192>t,S64>I(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)| t196>c,Y66>H(+ - -)|t199>c,Y67>H(+ - -)|t208>g,S70>A(- + -)|t246>c|a263>t,Y88>F(- + -)|c265>t|c276>t|a277>g,S93>G(+ + -)|c279>a,S93>G(+ + -)|c280>g,L94>V(+ - +)|c287>a,A96>D(- - -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)|t306>c| g313>a,A105>T(- - -)|g319>a|a320>c| 318 (320) 223 (225) 189 34 (36) 10 (12) 24 3 8 (9) 4 5 1 (2) 2 2 3 1 3 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 1 2 0 1 0 0 0 0 0 1 0 0 51 51 46 5 3 2 0 2 0 0 1 0 0 1 0 1 0 0 30 24 16 8 1 7 1 2 2 0 0 0 0 2 0 0 1 0 117 114 99 15 4 11 1 1 2 4 0 2 2 0 1 1 0 1 6 (8) 6 5 1 (3) 0 (2) 1 0 1 (2) 0 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgttcgacaactatgcc atgagctgggtccgccaggcaccaggaaaggggctggagtgggtctccagt attagtggtaatggagaaattgta caccacgcagacgccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtttttgcaaatgaatggagtgagagacgacgacacggccatttactactgt acgaaaacacgcgttgtgttgggaattacatttccgttctatatctatggtctggacgtc TKTRVVLGITFPFYIYGLDV IGHV3-23 IGHD3-10 IGHJ6 ggattcacgttcgacaactatgcc atgagctgggtccgccaggcaccaggaaaggggctggagtgggtctccagt attagtggtaatggagaaattgta caccacgcagacgccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtttttgcaaatgaatggagtgagagacgacgacacggccatttactactgt acgaaaacacgcgttgtgttgggaattacatttccgttctatatctatggtctggacgtc IGA1 33 213 19 14 11 18 8 15 7 3 13 10 JY8QFUQ01ALDY2 15423 productive Homsap IGHV4-59*03 F 899 91.16 196/215 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD4-23*01 ORF 3 8 7 9 8.7.9 [1.17.38.11] CARGVTPEGYW in-frame + tctcgggtgactccatcactcctaactcctggagctggatccggcagcccccagggaagggactggagtggattggttatatctattacagtggaatcaccaagcacaacccctccctcaagagtcgagtcgccatttcagtagacacgtccaagaaccaattttccctgaggctgagttctgtgaccgctgcggacacggccgtttatttctgtgcgaggggggtcactccggagggctactggggccagggaatcttagtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [273] t78>g|g83>a,G28>D(- - -)|g104>c,S35>T(+ - +)|a106>c,S36>P(+ - -)|g107>c,S36>P(+ - -)|t109>a,Y37>N(- - -)|a113>c,Y38>S(+ - -)|g162>t|g189>a|g191>t,S64>I(- - -)|c198>g,N66>K(+ - -)|t199>c,Y67>H(+ - -)|a229>g,T77>A(- - -)|a234>t|c261>t|a269>g,K90>R(+ + +)|c276>t|g303>t|a308>t,Y103>F(- + -)| t78>g| g83>a,G28>D(- - -)|g104>c,S35>T(+ - +)|a106>c,S36>P(+ - -)|g107>c,S36>P(+ - -)|t109>a,Y37>N(- - -)|a113>c,Y38>S(+ - -)| g162>t| g189>a|g191>t,S64>I(- - -)| c198>g,N66>K(+ - -)|t199>c,Y67>H(+ - -)|a229>g,T77>A(- - -)|a234>t|c261>t|a269>g,K90>R(+ + +)|c276>t|g303>t|a308>t,Y103>F(- + -)| 315 218 199 19 7 12 2 2 2 1 2 0 2 1 2 1 3 1 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 36 24 18 6 0 6 0 1 0 0 2 0 0 1 2 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 30 21 19 2 1 1 0 1 0 0 0 0 0 0 0 0 1 0 117 114 105 9 4 5 2 0 2 1 0 0 2 0 0 1 1 0 3 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,257-258(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctcg ggtgactccatcactcctaactcc tggagctggatccggcagcccccagggaagggactggagtggattggttat atctattacagtggaatcacc aagcacaacccctccctcaagagtcgagtcgccatttcagtagacacgtccaagaaccaattttccctgaggctgagttctgtgaccgctgcggacacggccgtttatttctgt gcgaggggggtcactccggagggctac ARGVTPEGY IGHV4-59 IGHD4-23 IGHJ4 ggtgactccatcactcctaactcc tggagctggatccggcagcccccagggaagggactggagtggattggttat atctattacagtggaatcacc aagcacaacccctccctcaagagtcgagtcgccatttcagtagacacgtccaagaaccaattttccctgaggctgagttctgtgaccgctgcggacacggccgtttatttctgt gcgaggggggtcactccggagggctac IGA1 18 210 7 11 4 10 3 8 5 1 5 7 JY8QFUQ01ALFPF 16926 productive Homsap IGHV4-61*02 F 920 90.95 201/221 nt NA Homsap IGHJ5*02 F 228 94.12 48/51 nt Homsap IGHD3-22*01 F 3 10 7 14 10.7.14 [1.17.38.11] CAREKVVIRPNWFDPW in-frame + tctctggtgactccatcaccagtggtaattattattggagttggatccggcagcccgccgagaagggactggagtggattgggcgtatctccatcggtgggatcaccaactacaatccctccctcaagagtcgagtcaccatactattagacacgtccagcaaccggttctccctgaagctcagctctgtgaccgccgcagacacggccgtgtattactgtgcgagagaaaaagtagtgatccgtcccaattggttcgacccctggggccagggagccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [295] g83>a,G28>D(- - -)|g92>c,S31>T(+ - +)|g107>a,S36>N(- - -)|c111>t|c114>t|c120>t|g140>a,G47>E(- - -)|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|c173>t,T58>I(- - -)|a175>g,S59>G(+ + -)|g191>t,S64>I(- - -)|c204>t|t235>c,S79>L(- - -)|c236>t,S79>L(- - -)|g238>t,V80>L(+ - +)|a251>g,K84>S(- - -)|g252>c,K84>S(- - -)|a257>g,Q86>R(+ - -)|g273>c| g83>a,G28>D(- - -)|g92>c,S31>T(+ - +)|g107>a,S36>N(- - -)|c111>t|c114>t| c120>t|g140>a,G47>E(- - -)| a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|c173>t,T58>I(- - -)|a175>g,S59>G(+ + -)|g191>t,S64>I(- - -)| c204>t|t235>c,S79>L(- - -)|c236>t,S79>L(- - -)|g238>t,V80>L(+ - +)|a251>g,K84>S(- - -)|g252>c,K84>S(- - -)|a257>g,Q86>R(+ - -)|g273>c| 320 229 209 20 5 15 3 3 6 2 1 0 0 0 3 0 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 25 5 2 3 0 2 2 0 0 0 0 0 1 0 0 0 51 51 49 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 30 21 16 5 0 5 1 0 1 1 1 0 0 0 0 0 1 0 117 114 106 8 2 6 2 0 2 1 0 0 0 0 2 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatcaccagtggtaattattat tggagttggatccggcagcccgccgagaagggactggagtggattgggcgt atctccatcggtgggatcacc aactacaatccctccctcaagagtcgagtcaccatactattagacacgtccagcaaccggttctccctgaagctcagctctgtgaccgccgcagacacggccgtgtattactgt gcgagagaaaaagtagtgatccgtcccaattggttcgacccc AREKVVIRPNWFDP IGHV4-61 IGHD3-22 IGHJ5 ggtgactccatcaccagtggtaattattat tggagttggatccggcagcccgccgagaagggactggagtggattgggcgt atctccatcggtgggatcacc aactacaatccctccctcaagagtcgagtcaccatactattagacacgtccagcaaccggttctccctgaagctcagctctgtgaccgccgcagacacggccgtgtattactgt gcgagagaaaaagtagtgatccgtcccaattggttcgacccc IGG1 20 216 14 6 9 14 5 6 3 2 7 8 JY8QFUQ01ALJ94 17222 productive Homsap IGHV3-7*01 F 885 90.65 194/214 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD6-19*01 F 1 8 8 10 8.8.10 [0.17.38.11] CAGGRGWLIDYW in-frame + tggtttcagcttaagtgactattggatgaactgggtccgccaggctccagggaaggggctcgagtgggtggccatcataaagaaagatggaagtgaagaactctatttggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcactgtatctggaaatgaacagcctgagccccgaggacacggctgtatatttctgtgcggggggcagaggctggcttattgactactggggcctgggaaccgcggtcatcgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 79 94 [276] a81>t|c86>g,T29>S(+ - +)|t90>a,F30>L(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g119>a,S40>N(- - -)|g150>c|a164>t,N55>I(- - -)|c172>a,Q58>K(+ - -)|g192>a|a193>g,K65>E(+ - -)|t196>c,Y66>L(- - -)|a197>t,Y66>L(- - -)|g202>t,V68>L(+ - +)|a232>g,I78>V(+ - +)|c268>g,Q90>E(+ + -)|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|g303>a|a308>t,Y103>F(- + -)| a81>t|c86>g,T29>S(+ - +)|t90>a,F30>L(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g119>a,S40>N(- - -)|g150>c|a164>t,N55>I(- - -)| c172>a,Q58>K(+ - -)|g192>a|a193>g,K65>E(+ - -)| t196>c,Y66>L(- - -)|a197>t,Y66>L(- - -)|g202>t,V68>L(+ - +)|a232>g,I78>V(+ - +)|c268>g,Q90>E(+ + -)|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|g303>a|a308>t,Y103>F(- + -)| a316>g,R106>G(- - -)|a318>g,R106>G(- - -)|a320>g| 315 (320) 217 (222) 197 (199) 20 (23) 4 (5) 16 (18) 3 (6) 4 0 1 1 1 4 1 2 2 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 1 1 0 0 0 0 1 1 0 1 0 0 51 51 48 3 1 2 0 1 0 0 0 0 1 0 1 0 0 0 30 24 21 3 1 2 1 1 0 0 0 1 0 0 0 0 0 0 117 114 105 9 1 8 1 1 0 1 1 0 2 0 1 1 1 0 3 (8) 3 3 (5) 0 (3) 0 (1) 0 (2) 0 (3) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t ggtttcagcttaagtgactattgg atgaactgggtccgccaggctccagggaaggggctcgagtgggtggccatc ataaagaaagatggaagtgaagaa ctctatttggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcactgtatctggaaatgaacagcctgagccccgaggacacggctgtatatttctgt gcggggggcagaggctggcttattgactac AGGRGWLIDY IGHV3-7 IGHD6-19 IGHJ4 ggtttcagcttaagtgactattgg atgaactgggtccgccaggctccagggaaggggctcgagtgggtggccatc ataaagaaagatggaagtgaagaa ctctatttggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcactgtatctggaaatgaacagcctgagccccgaggacacggctgtatatttctgt gcggggggcagaggctggcttattgactac IGG1 20 213 8 12 4 10 4 10 2 2 10 6 JY8QFUQ01ALJS1 18149 productive Homsap IGHV3-21*04 F, or Homsap IGHV3-23*01 F or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 909 91.24 198/217 nt NA Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [1.17.38.11] CAKDGARGGGSCFNSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcaccttcagtagttatagcatgcactgggtccgccaggctccagggaagggactggagtgggtctcctccattagtagtaatggtgctaacacatactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgtgcgaaagatggcgctagaggtggcggcagttgctttaactcctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [291] c108>t|a118>c,N40>H(- - -)|g147>a|a162>c|g176>a,S59>N(- - -)|a184>g,S62>G(+ + -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t190>a,Y64>N(- - -)|t194>c,I65>T(- - -)|a210>c|c246>t|g247>t,A83>F(+ - -)|c248>t,A83>F(+ - -)|t256>a,S86>T(+ - +)|a258>g,S86>T(+ - +)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)|g317>a,R106>K(+ + +)| c108>t| a118>c,N40>H(- - -)|g147>a|a162>c| g176>a,S59>N(- - -)|a184>g,S62>G(+ + -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t190>a,Y64>N(- - -)|t194>c,I65>T(- - -)| a210>c|c246>t|g247>t,A83>F(+ - -)|c248>t,A83>F(+ - -)|t256>a,S86>T(+ - +)|a258>g,S86>T(+ - +)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| g317>a,R106>K(+ + +)| 320 225 205 20 5 15 3 4 3 1 4 0 0 2 2 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 0 0 2 0 0 0 0 0 0 0 30 24 18 6 0 6 2 1 0 1 0 0 0 1 1 0 0 0 117 114 105 9 2 7 1 1 2 0 2 0 0 1 1 0 1 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagttatagc atgcactgggtccgccaggctccagggaagggactggagtgggtctcctcc attagtagtaatggtgctaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtggcggcagttgctttaactcc AKDGARGGGSCFNS IGHV3-21 IGHD5-24 IGHJ4 ggattcaccttcagtagttatagc atgcactgggtccgccaggctccagggaagggactggagtgggtctcctcc attagtagtaatggtgctaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtggcggcagttgctttaactcc IGG2 19 213 10 9 6 9 4 10 4 1 8 6 JY8QFUQ01ALKNG 7298 productive Homsap IGHV3-7*01 F 908 91.63 197/215 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-21*02 F 2 8 8 18 8.8.18 [0.17.38.11] CSRHGGYTKLYNQNVYLDYW in-frame - ctggattcacatttagcaacttttggatgagctgggtccgccagactccagggaaggggctggagtgggtggccaaaataaacccagtcggaagtgagaaatactatgtggactctgtgaagggccgattcaccacctccagagacaactctagaaactcgctgtgtctgcaaatggacagcctgagagccgaggacacggctgtgtattactgttcgagacatggaggttacacgaaactctacaatcaaaatgtctaccttgactactggggccagggaaccctggtcaccgtctccccagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [300, 299] c87>a|t105>c|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)|g133>a,A45>T(- - -)|c165>a,N55>K(+ - -)|g171>c,K57>N(+ - -)|a173>c,Q58>P(- - -)|a176>t,D59>V(- - -)|t177>c,D59>V(- - -)|t233>c,I78>T(- - -)|g247>t,A83>S(- + -)|c249>t,A83>S(- + -)|a251>g,K84>R(+ + +)|g252>a,K84>R(+ + +)|a258>g|a263>g,Y88>C(- - -)|a274>g,N92>D(+ + -)|g313>t,A105>S(- + -)| c87>a|t105>c|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)| g133>a,A45>T(- - -)|c165>a,N55>K(+ - -)| g171>c,K57>N(+ - -)|a173>c,Q58>P(- - -)|a176>t,D59>V(- - -)|t177>c,D59>V(- - -)| t233>c,I78>T(- - -)|g247>t,A83>S(- + -)|c249>t,A83>S(- + -)|a251>g,K84>R(+ + +)|g252>a,K84>R(+ + +)|a258>g|a263>g,Y88>C(- - -)|a274>g,N92>D(+ + -)| g313>t,A105>S(- + -)|g319>c| 318 (320) 221 (223) 202 (203) 19 (20) 3 (4) 16 4 3 1 3 1 2 2 0 1 (2) 0 2 0 78 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 1 0 1 0 1 1 0 0 0 0 0 51 51 49 2 0 2 0 1 0 0 0 1 0 0 0 0 0 0 30 24 20 4 0 4 0 0 0 1 1 0 1 0 1 0 0 0 117 114 106 8 1 7 4 1 1 1 0 0 0 0 0 0 1 0 6 (8) 6 5 (6) 1 (2) 0 (1) 1 0 0 0 0 0 0 0 0 0 (1) 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ct ggattcacatttagcaacttttgg atgagctgggtccgccagactccagggaaggggctggagtgggtggccaaa ataaacccagtcggaagtgagaaa tactatgtggactctgtgaagggccgattcaccacctccagagacaactctagaaactcgctgtgtctgcaaatggacagcctgagagccgaggacacggctgtgtattactgt tcgagacatggaggttacacgaaactctacaatcaaaatgtctaccttgactac SRHGGYTKLYNQNVYLDY IGHV3-7 IGHD2-21 IGHJ4 ggattcacatttagcaacttttgg atgagctgggtccgccagactccagggaaggggctggagtgggtggccaaa ataaacccagtcggaagtgagaaa tactatgtggactctgtgaagggccgattcaccacctccagagacaactctagaaactcgctgtgtctgcaaatggacagcctgagagccgaggacacggctgtgtattactgt tcgagacatggaggttacacgaaactctacaatcaaaatgtctaccttgactac IGA2 18 213 11 7 4 8 7 10 1 2 9 6 JY8QFUQ01ALMSJ 15781 productive (see comment) Homsap IGHV3-66*01 F, or Homsap IGHV3-66*02 F or Homsap IGHV3-66*04 F 971 92.86 208/224 nt 92.41 207/224 nt Homsap IGHJ4*02 F 114 70.83 34/48 nt NA 7 7 8 7.7.8 [5.17.38.11] CARGAPFYDS (see Functionality comment) in-frame - Potentially, because of detected insertion/deletion, IMGT/JunctionAnalysis gives no results for this JUNCTION in CDR1-IMGT, from codon 37 of V-REGION: 1 nucleotides (from position 35 in the user submitted sequence), (cause frameshift) gcctctgcagcctctggattcattgtcaatagcactacatgagttgggtccgccaggctccagggaaggggctggactgcgtctcagttatttatcccggtggtagcacatactatgcagactccgtgaagggccgattcaccatctccagagacatctccaagaacacactgtatcttcaaatgaacagcctgagagccgaggacacggctgtatattactgtgcgagaggggccccattttatgatagtgttaagggaaccctggtcaccgtctcttcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [280] t64>g,S22>A(- + -)|g68>c,C23>S(- - -)|c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g104>a,S35>N(- - -)|c120>t|g153>c,E51>D(+ - +)|g156>c,W52>C(+ - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c201>t|a223>c|a245>t,N82>I(- - -)|t246>c,N82>I(- - -)|g258>a|g303>a| t64>g,S22>A(- + -)|g68>c,C23>S(- - -)| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g104>a,S35>N(- - -)| c120>t|g153>c,E51>D(+ - +)|g156>c,W52>C(+ - -)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)| c201>t|a223>c|a245>t,N82>I(- - -)|t246>c,N82>I(- - -)|g258>a|g303>a| a320>g| 319 (320) 231 (232) 215 16 (17) 5 (6) 11 0 (1) 3 4 1 2 0 1 0 4 0 0 1 78 15 13 2 0 2 0 0 0 0 0 0 0 0 1 0 0 1 36 23 20 3 0 3 0 1 2 0 0 0 0 0 0 0 0 0 51 51 48 3 1 2 0 0 1 0 0 0 0 0 2 0 0 0 30 21 19 2 0 2 0 0 0 0 1 0 0 0 1 0 0 0 117 114 108 6 4 2 0 2 1 1 1 0 1 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|ggca,220-223(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctctgcagcctct ggattcattgtcaatagcactac atgagttgggtccgccaggctccagggaaggggctggactgcgtctcagtt atttatcccggtggtagcaca tactatgcagactccgtgaagggccgattcaccatctccagagacatctccaagaacacactgtatcttcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgagaggggccccattttatgat ARGAPFYD IGHV3-66 IGHJ4 ggattcattgtcaatagcactac atgagttgggtccgccaggctccagggaaggggctggactgcgtctcagtt atttatcccggtggtagcaca tactatgcagactccgtgaagggccgattcaccatctccagagacatctccaagaacacactgtatcttcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgagaggggccccattttatgat IGG2 14 209 8 6 7 10 1 4 5 0 4 5 JY8QFUQ01ALN7I 9955 productive Homsap IGHV4-61*03 F 888 89.55 197/220 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD5-12*01 F 1 10 7 12 10.7.12 [1.17.38.11] CTRHPKSNVEGDSW in-frame + ccctggtggctccgtcagcagtggtagttatttctggagctggatccggcagcccccagggcaggggctggaatggatcggttatgtctataacaatgggaacaccaattacaacccttccctcaagagtcgagtcaccatttctatagacacgtccgagaatcacgtctccctgaagctggcctctgtgaccgctgcggacacggccgtgtactactgtacgaggcacccgaagtctaatgtagagggtgactcctggggccagggagccccggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [287] t76>c,S26>P(+ - -)|c111>t|a113>t,Y38>F(- + -)|a142>c,K48>Q(+ - -)|a147>g|g153>a|t159>c|g162>t|a166>g,I56>V(+ - +)|t172>a,Y58>N(- - -)|g176>a,S59>N(- - -)|g191>a,S64>N(- - -)|c198>t|c207>t|a234>t|a237>t|g238>a,V80>I(+ - +)|a250>g,K84>E(+ - -)|c255>t|t259>g,F87>V(+ - -)|a274>g,S92>A(- + -)|g275>c,S92>A(- + -)|t306>c|g313>a,A105>T(- - -)| t76>c,S26>P(+ - -)| c111>t|a113>t,Y38>F(- + -)| a142>c,K48>Q(+ - -)|a147>g|g153>a|t159>c|g162>t| a166>g,I56>V(+ - +)|t172>a,Y58>N(- - -)|g176>a,S59>N(- - -)|g191>a,S64>N(- - -)| c198>t|c207>t|a234>t|a237>t|g238>a,V80>I(+ - +)|a250>g,K84>E(+ - -)|c255>t|t259>g,F87>V(+ - -)|a274>g,S92>A(- + -)|g275>c,S92>A(- + -)|t306>c| g313>a,A105>T(- - -)|a318>g|g319>c| 317 (320) 225 (228) 201 (202) 24 (26) 11 (13) 13 4 (5) 5 4 3 1 0 3 1 1 (2) 0 1 1 78 4 3 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 36 30 28 2 1 1 0 0 1 0 0 0 1 0 0 0 0 0 51 51 46 5 4 1 1 1 0 1 1 0 0 0 0 0 1 0 30 21 17 4 0 4 1 2 0 0 0 0 0 1 0 0 0 0 117 114 103 11 6 5 2 1 3 1 0 0 2 0 1 0 0 1 5 (8) 5 4 (5) 1 (3) 0 (2) 1 0 (1) 1 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ccct ggtggctccgtcagcagtggtagttatttc tggagctggatccggcagcccccagggcaggggctggaatggatcggttat gtctataacaatgggaacacc aattacaacccttccctcaagagtcgagtcaccatttctatagacacgtccgagaatcacgtctccctgaagctggcctctgtgaccgctgcggacacggccgtgtactactgt acgaggcacccgaagtctaatgtagagggtgactcc TRHPKSNVEGDS IGHV4-61 IGHD5-12 IGHJ4 ggtggctccgtcagcagtggtagttatttc tggagctggatccggcagcccccagggcaggggctggaatggatcggttat gtctataacaatgggaacacc aattacaacccttccctcaagagtcgagtcaccatttctatagacacgtccgagaatcacgtctccctgaagctggcctctgtgaccgctgcggacacggccgtgtactactgt acgaggcacccgaagtctaatgtagagggtgactcc IGA2 22 216 14 8 8 10 6 12 10 1 6 5 JY8QFUQ01ALPR0 20294 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 814 86.57 187/216 nt 85.65 185/216 nt Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD3-16*01 F 1 8 7 14 8.7.14 [2.17.38.11] CARCKSAGLVDWFDPW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 62 to 62 in the user submitted sequence), (cause frameshift); in CDR2-IMGT, from codon 57 of V-REGION: 3 nucleotides (from position 85 in the user submitted sequence), (do not cause frameshift) gcctctggattcagcattagcgactatgccatggcctgggtccgccaggctccagggaaggGggccggagtgggtctcaagtgttactaatggttttggcacatactacgcagactctgtgaagggccggttcaccgcctccagagacaattccaagaacgcattgttcttggaaatgaacatcctcagagccgaggacacggccgtatattattgtgcgaggtgtaagtcggccggtttggtggactggttcgacccctggggccagggaaccctggtcatcgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [291] c86>g,T29>S(+ - +)|t88>a,F30>I(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|t149>c,L50>P(- - -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|a166>g,I56>V(+ - +)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|g176>a,S59>N(- - -)|g187>t,G63>F(- - -)|g188>t,G63>F(- - -)|a190>g,S64>G(+ + -)|c210>t|a232>g,I78>A(+ - +)|t233>c,I78>A(+ - +)|a256>g,T86>A(- - -)|g258>a,T86>A(- - -)|c259>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|c268>g,Q90>E(+ + -)|g278>t,S93>I(- - -)|g282>c|c309>t| c86>g,T29>S(+ - +)|t88>a,F30>I(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|t149>c,L50>P(- - -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| a166>g,I56>V(+ - +)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|g176>a,S59>N(- - -)|g187>t,G63>F(- - -)|g188>t,G63>F(- - -)|a190>g,S64>G(+ + -)| c210>t|a232>g,I78>A(+ - +)|t233>c,I78>A(+ - +)|a256>g,T86>A(- - -)|g258>a,T86>A(- - -)|c259>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|c268>g,Q90>E(+ + -)|g278>t,S93>I(- - -)|g282>c|c309>t| a317>g,K106>R(+ + +)|a318>g,K106>R(+ + +)|g319>t|a320>g| 316 (320) 220 (224) 191 29 (33) 5 (7) 24 (26) 6 (9) 5 4 3 0 0 1 1 3 3 3 (4) 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 0 0 0 0 0 1 0 1 0 0 51 51 46 5 0 5 1 1 0 1 0 0 0 0 1 1 0 0 30 21 14 7 0 7 2 2 0 0 0 0 0 0 1 0 2 0 117 114 101 13 5 8 2 1 4 2 0 0 1 0 1 1 1 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (3) 0 0 0 0 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcagcattagcgactatgcc atggcctgggtccgccaggctccagggaaggggccggagtgggtctcaagt gttactaatggttttggcaca tactacgcagactctgtgaagggccggttcaccgcctccagagacaattccaagaacgcattgttcttggaaatgaacatcctcagagccgaggacacggccgtatattattgt gcgaggtgtaagtcggccggtttggtggactggttcgacccc ARCKSAGLVDWFDP IGHV3-23 IGHD3-16 IGHJ5 ggattcagcattagcgactatgcc atggcctgggtccgccaggctccagggaaggggccggagtgggtctcaagt gttactaatggttttggcaca tactacgcagactctgtgaagggccggttcaccgcctccagagacaattccaagaacgcattgttcttggaaatgaacatcctcagagccgaggacacggccgtatattattgt gcgaggtgtaagtcggccggtttggtggactggttcgacccc IGG1 29 210 18 11 9 18 9 11 5 0 13 11 JY8QFUQ01ALW0Z 15340 productive Homsap IGHV3-7*01 F 945 93.09 202/217 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-3*01 F 2 8 8 10 8.8.10 [1.17.38.11] CASDLGGQKSYW in-frame + ctctggattcacctttagtgactattggatgagctgggtccgccaggctccagggaaggggctagagtgggtggccaacataaatagagatggaagtgagcaacactatgtggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtatttgcaaatgaacagtctgagagccgaggacacggctgtctattactgtgcaagcgatttgggtggtcagaaaagttactggggccagggagtcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [278] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g150>a|g171>t,K57>N(+ - -)|c172>a,Q58>R(+ - -)|a173>g,Q58>R(+ - -)|a193>c,K65>Q(+ - -)|t196>c,Y66>H(+ - -)|t210>c|t256>a,S86>K(- - -)|c257>a,S86>K(- - -)|a258>g,S86>K(- - -)|c265>t|c279>t|g303>c|g315>a|a318>c,R106>S(- - -)| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g150>a| g171>t,K57>N(+ - -)|c172>a,Q58>R(+ - -)|a173>g,Q58>R(+ - -)|a193>c,K65>Q(+ - -)| t196>c,Y66>H(+ - -)|t210>c|t256>a,S86>K(- - -)|c257>a,S86>K(- - -)|a258>g,S86>K(- - -)|c265>t|c279>t|g303>c| g315>a|a318>c,R106>S(- - -)| 320 225 208 17 6 11 3 3 2 2 2 2 0 1 1 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 0 0 1 1 0 0 0 0 1 0 117 114 106 8 4 4 1 0 2 2 0 1 0 1 1 0 0 0 8 8 6 2 1 1 0 1 0 0 1 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtgactattgg atgagctgggtccgccaggctccagggaaggggctagagtgggtggccaac ataaatagagatggaagtgagcaa cactatgtggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtatttgcaaatgaacagtctgagagccgaggacacggctgtctattactgt gcaagcgatttgggtggtcagaaaagttac ASDLGGQKSY IGHV3-7 IGHD3-3 IGHJ4 ggattcacctttagtgactattgg atgagctgggtccgccaggctccagggaaggggctagagtgggtggccaac ataaatagagatggaagtgagcaa cactatgtggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtatttgcaaatgaacagtctgagagccgaggacacggctgtctattactgt gcaagcgatttgggtggtcagaaaagttac IGA1 15 213 9 6 4 8 5 7 5 0 4 6 JY8QFUQ01ALZYF 14835 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 863 89.3 192/215 nt 88.84 191/215 nt Homsap IGHJ3*02 F 232 96 48/50 nt Homsap IGHD2-15*01 F 2 8 7 20 8.7.20 [1.17.38.11] CARDPRDYCSGGYCFVGAFDIW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 78 of V-REGION: 'C' (1 nucleotides at position 135 to 135 in the user submitted sequence), (cause frameshift); tctctggtggctccatgaggaattattactggagctggatccggcagtccccagggaagggactggagttgatagggactgtctattacactgggcgcacggagtacaacccctccctcaagagtcgactcaccCttatcactagacacgtccaagaaccagttctccctaaagctgggctctgtgaccgctgcggactcggccatttattactgtgcgagagacccccgagactattgtagtggtggttactgttttgttggtgcttttgatatctggggccaagggacaaaggtcaccgtctcttcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [308] c90>g,I30>M(+ + -)|t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t|c133>t,P45>S(+ - -)|g155>t,W52>L(+ - -)|t159>a|t163>a,Y55>T(+ - -)|a164>c,Y55>T(+ - -)|a166>g,I56>V(+ - +)|g176>c,S59>T(+ - +)|a190>c,S64>R(- - -)|c195>g|a196>g,N66>E(+ - -)|c198>g,N66>E(+ - -)|g226>c,V76>L(+ - +)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|g267>a|a274>g,S92>G(+ + -)|a295>t,T99>S(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| c90>g,I30>M(+ + -)|t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t| c133>t,P45>S(+ - -)|g155>t,W52>L(+ - -)|t159>a|t163>a,Y55>T(+ - -)|a164>c,Y55>T(+ - -)| a166>g,I56>V(+ - +)|g176>c,S59>T(+ - +)|a190>c,S64>R(- - -)|c195>g| a196>g,N66>E(+ - -)|c198>g,N66>E(+ - -)|g226>c,V76>L(+ - +)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|g267>a|a274>g,S92>G(+ + -)|a295>t,T99>S(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| 320 223 200 23 4 19 3 3 2 0 2 0 2 2 3 3 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 1 0 0 0 0 0 0 1 0 1 51 51 46 5 1 4 0 0 1 0 1 0 0 2 0 0 1 0 30 21 17 4 1 3 1 0 0 0 1 0 0 0 1 1 0 0 117 114 104 10 1 9 2 2 0 0 0 0 2 0 2 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatgaggaattattac tggagctggatccggcagtccccagggaagggactggagttgatagggact gtctattacactgggcgcacg gagtacaacccctccctcaagagtcgactcaccttatcactagacacgtccaagaaccagttctccctaaagctgggctctgtgaccgctgcggactcggccatttattactgt gcgagagacccccgagactattgtagtggtggttactgttttgttggtgcttttgatatc ARDPRDYCSGGYCFVGAFDI IGHV4-59 IGHD2-15 IGHJ3 ggtggctccatgaggaattattac tggagctggatccggcagtccccagggaagggactggagttgatagggact gtctattacactgggcgcacg gagtacaacccctccctcaagagtcgactcaccttatcactagacacgtccaagaaccagttctccctaaagctgggctctgtgaccgctgcggactcggccatttattactgt gcgagagacccccgagactattgtagtggtggttactgttttgttggtgcttttgatatc IGG1 23 210 8 15 5 13 3 10 2 2 13 6 JY8QFUQ01AM056 20954 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 905 90.83 198/218 nt 90.37 197/218 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD1-14*01 ORF 3 8 8 9 8.8.9 [1.17.38.11] CAKEEVPNDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'A' (1 nucleotides at position 15 to 15 in the user submitted sequence), (cause frameshift); tctctggattcaccAtttagcaatgctgccatgacgtgggtccgccaggctccagggaaggggctagagtgggtctcaggtattagtattagtggtgatagaacatattacgcagactccgtgaagggccggttcaccatctctagagacaattccaagaataccgtgtatctgcaaatgaacagcctgagagccgaggacacggccatatattattgtgcgaaagaggaggtcccgaatgattattggggccagggaactctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [277] c74>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>g,Y37>A(- - -)|a110>c,Y37>A(- - -)|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|g150>a|c164>g,A55>G(- + -)|g172>a,G58>I(- - -)|g173>t,G58>I(- - -)|g188>a,G63>D(- - -)|c192>a,S64>R(- - -)|c198>t|c237>t|c255>t|g258>c|c259>g,L87>V(+ - +)|g301>a,V101>I(+ - +)|c309>t| c74>t| g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>g,Y37>A(- - -)|a110>c,Y37>A(- - -)| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|g150>a|c164>g,A55>G(- + -)| g172>a,G58>I(- - -)|g173>t,G58>I(- - -)|g188>a,G63>D(- - -)|c192>a,S64>R(- - -)| c198>t|c237>t|c255>t|g258>c|c259>g,L87>V(+ - +)|g301>a,V101>I(+ - +)|c309>t| 320 226 206 20 7 13 0 5 6 0 1 1 0 0 2 3 1 1 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 1 0 1 0 0 0 0 0 0 1 51 51 47 4 1 3 0 1 0 0 0 0 0 0 1 2 0 0 30 24 20 4 0 4 0 2 0 0 0 1 0 0 0 0 1 0 117 114 107 7 5 2 0 1 4 0 0 0 0 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tctct ggattcacctttagcaatgctgcc atgacgtgggtccgccaggctccagggaaggggctagagtgggtctcaggt attagtattagtggtgatagaaca tattacgcagactccgtgaagggccggttcaccatctctagagacaattccaagaataccgtgtatctgcaaatgaacagcctgagagccgaggacacggccatatattattgt gcgaaagaggaggtcccgaatgattat AKEEVPNDY IGHV3-23 IGHD1-14 IGHJ4 ggattcacctttagcaatgctgcc atgacgtgggtccgccaggctccagggaaggggctagagtgggtctcaggt attagtattagtggtgatagaaca tattacgcagactccgtgaagggccggttcaccatctctagagacaattccaagaataccgtgtatctgcaaatgaacagcctgagagccgaggacacggccatatattattgt gcgaaagaggaggtcccgaatgattat IGA1 19 213 10 9 10 17 0 2 6 0 5 8 JY8QFUQ01AM0T6 19376 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 881 90.23 194/215 nt 89.3 192/215 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD4-23*01 ORF 3 8 7 12 8.7.12 [1.17.38.11] CASSVFSPGGFDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 54 of V-REGION: 'T' (1 nucleotides at position 77 to 77 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 59 of V-REGION: 'G' (1 nucleotides at position 92 to 92 in the user submitted sequence), (cause frameshift); tttcggggttcaccgtcagtagcaagtacatgacctgggtccgccaggctccggggaagggactggagtctgtctcTggttttttatagcgGgtgatcaaacatactacgcagactccgtgaggggccgattcaccatctccatagacaattccaagaacacactgtatcttcaaatgaacggcctgcgagccgaggacacggccgtgtattattgtgcgagctccgtgtttagtcccggaggctttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [284] c74>t|c75>t|t78>g|c111>g,N37>K(+ - -)|g119>c,S40>T(+ - +)|a138>g|g147>a|g155>c,W52>S(- - -)|g156>t,W52>S(- - -)|a162>g|a166>t,I56>F(+ - -)|g188>a,G63>D(- - -)|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>a,S64>Q(- - -)|a215>g,K72>R(+ + +)|g239>t,R80>I(- + -)|g258>a|a277>g,S93>G(+ + -)|a283>c|c309>t| c74>t|c75>t|t78>g| c111>g,N37>K(+ - -)| g119>c,S40>T(+ - +)|a138>g|g147>a|g155>c,W52>S(- - -)|g156>t,W52>S(- - -)|a162>g| a166>t,I56>F(+ - -)|g188>a,G63>D(- - -)|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>a,S64>Q(- - -)| a215>g,K72>R(+ + +)|g239>t,R80>I(- + -)|g258>a|a277>g,S93>G(+ + -)|a283>c|c309>t| a318>c,R106>S(- - -)|g319>t|a320>c| 317 (320) 220 (223) 199 21 (24) 9 (11) 12 (13) 4 4 3 0 2 (4) 1 1 0 2 1 2 (3) 1 78 5 2 3 3 0 0 0 2 0 0 0 0 0 0 0 0 1 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 45 6 3 3 2 1 0 0 0 0 0 0 2 0 1 0 30 21 16 5 0 5 0 2 0 0 1 1 1 0 0 0 0 0 117 114 108 6 3 3 2 1 1 0 1 0 0 0 0 0 1 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 0 0 0 0 (2) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tttcg gggttcaccgtcagtagcaagtac atgacctgggtccgccaggctccggggaagggactggagtctgtctcggtt ttttatagcggtgatcaaaca tactacgcagactccgtgaggggccgattcaccatctccatagacaattccaagaacacactgtatcttcaaatgaacggcctgcgagccgaggacacggccgtgtattattgt gcgagctccgtgtttagtcccggaggctttgactac ASSVFSPGGFDY IGHV3-53 IGHD4-23 IGHJ4 gggttcaccgtcagtagcaagtac atgacctgggtccgccaggctccggggaagggactggagtctgtctcggtt ttttatagcggtgatcaaaca tactacgcagactccgtgaggggccgattcaccatctccatagacaattccaagaacacactgtatcttcaaatgaacggcctgcgagccgaggacacggccgtgtattattgt gcgagctccgtgtttagtcccggaggctttgactac IGA2 18 210 9 9 5 11 4 7 6 0 6 6 JY8QFUQ01AM4ZR 14913 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 855 88.48 192/217 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD5-18*01 F 3 8 8 21 8.8.21 [1.17.38.11] CAKDSRAGGYPNGYFYYSGLDVW in-frame + ctctggacgctccttgagaagctttggcatgcactgggtccgccaggctccaggcaagggactggagtgggtggcacttacttcgtatgacggaaataggaaatattatgcagactccgtgaagggccgattcaccatctccagagacaactccaagaatacgttatttctgcaaatggacagtctgagagctgaggacacggctctttattactgtgcgaaagattcgcgagcgggtggttatccaaatggatacttctattactctggtctggacgtctggggccaagggacaatagtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccggaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [312] t82>c,F28>R(- - -)|t83>g,F28>R(- - -)|a85>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|t105>a,S35>R(- - -)|a110>t,Y37>F(- + -)|g147>a|g163>c,V55>L(+ - +)|t167>c,I56>T(- - -)|a168>t,I56>T(- - -)|a171>g|t177>c|g188>a,S63>N(- - -)|a191>g,N64>R(+ - -)|t192>g,N64>R(+ - -)|c198>t|t246>c|c255>t|c259>t|g261>a|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)|g317>a,R106>K(+ + +)| t82>c,F28>R(- - -)|t83>g,F28>R(- - -)|a85>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|t105>a,S35>R(- - -)|a110>t,Y37>F(- + -)| g147>a|g163>c,V55>L(+ - +)| t167>c,I56>T(- - -)|a168>t,I56>T(- - -)|a171>g|t177>c|g188>a,S63>N(- - -)|a191>g,N64>R(+ - -)|t192>g,N64>R(+ - -)| c198>t|t246>c|c255>t|c259>t|g261>a|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)| g317>a,R106>K(+ + +)| 320 225 199 26 9 17 3 4 4 4 0 0 4 1 2 1 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 0 0 1 0 0 2 1 0 1 0 1 51 51 49 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 30 24 17 7 2 5 2 1 0 2 0 0 1 0 0 0 0 1 117 114 104 10 6 4 1 1 4 1 0 0 1 0 1 0 1 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggacgctccttgagaagctttggc atgcactgggtccgccaggctccaggcaagggactggagtgggtggcactt acttcgtatgacggaaataggaaa tattatgcagactccgtgaagggccgattcaccatctccagagacaactccaagaatacgttatttctgcaaatggacagtctgagagctgaggacacggctctttattactgt gcgaaagattcgcgagcgggtggttatccaaatggatacttctattactctggtctggacgtc AKDSRAGGYPNGYFYYSGLDV IGHV3-30 IGHD5-18 IGHJ6 ggacgctccttgagaagctttggc atgcactgggtccgccaggctccaggcaagggactggagtgggtggcactt acttcgtatgacggaaataggaaa tattatgcagactccgtgaagggccgattcaccatctccagagacaactccaagaatacgttatttctgcaaatggacagtctgagagctgaggacacggctctttattactgt gcgaaagattcgcgagcgggtggttatccaaatggatacttctattactctggtctggacgtc IGG1 25 213 14 11 7 11 7 14 7 2 5 11 JY8QFUQ01AM701 6646 productive (see comment) Homsap IGHV1-46*01 F, or Homsap IGHV1-46*03 F 885 90.65 194/214 nt 90.19 193/214 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-10*01 F 2 8 8 14 8.8.14 [0.17.38.11] CARELGGSGTCGFDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 45 to 45 in the user submitted sequence), (cause frameshift); tggatacaccttcaccaggcactatatgcactgggtgcgacaggCcccctggacaaggacttgagtacatgggagtaatcaaccctagtggtggcgacacaagctacgcacagaggttccggggcagagtcgccgtgaccagagacacgtccacgagcacagtctatatggacttgagcagcctgagacctgaggacacggccatgtattattgtgcgagagagttgggaggttcggggacttgcggctttgactactggggccagggaagcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtcagggcttcttccc NA IGA1 100 100 [288] c108>g,S36>R(- - -)|t109>c,Y37>H(+ - -)|g147>a|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|a163>g,I55>V(+ - +)|t189>c|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a209>g,K70>R(+ + +)|a215>g,Q72>R(+ - -)|a229>g,T77>A(- - -)|a232>g,M78>V(+ - -)|g240>a|c264>t|g270>c,E90>D(+ - +)|c271>t|t286>c,S96>P(+ - -)|g301>a,V101>M(+ - -)|c309>t| c108>g,S36>R(- - -)|t109>c,Y37>H(+ - -)| g147>a|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|a163>g,I55>V(+ - +)| t189>c|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a209>g,K70>R(+ + +)|a215>g,Q72>R(+ - -)|a229>g,T77>A(- - -)|a232>g,M78>V(+ - -)|g240>a|c264>t|g270>c,E90>D(+ - +)|c271>t|t286>c,S96>P(+ - -)|g301>a,V101>M(+ - -)|c309>t| 320 222 202 20 6 14 6 5 3 3 0 0 0 0 2 1 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 1 0 0 0 0 0 1 0 0 51 51 47 4 1 3 1 2 0 0 0 0 0 0 1 0 0 0 30 24 21 3 1 2 1 1 0 1 0 0 0 0 0 0 0 0 117 114 103 11 4 7 4 2 3 1 0 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,162-163(FR2)|ta,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,62-63(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,164-165(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggca,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|aacc,169-172(CDR2)|agct,196-199(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcaccaggcactat atgcactgggtgcgacaggcccctggacaaggacttgagtacatgggagta atcaaccctagtggtggcgacaca agctacgcacagaggttccggggcagagtcgccgtgaccagagacacgtccacgagcacagtctatatggacttgagcagcctgagacctgaggacacggccatgtattattgt gcgagagagttgggaggttcggggacttgcggctttgactac ARELGGSGTCGFDY IGHV1-46 IGHD3-10 IGHJ4 ggatacaccttcaccaggcactat atgcactgggtgcgacaggcccctggacaaggacttgagtacatgggagta atcaaccctagtggtggcgacaca agctacgcacagaggttccggggcagagtcgccgtgaccagagacacgtccacgagcacagtctatatggacttgagcagcctgagacctgaggacacggccatgtattattgt gcgagagagttgggaggttcggggacttgcggctttgactac IGA1 20 213 17 3 8 11 9 9 5 1 10 4 JY8QFUQ01AMBSZ 20331 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD4-23*01 ORF 3 8 8 14 8.8.14 [1.17.38.11] CAKEGPTLLTRPPLNW in-frame + ctctggattcacctttagcagctatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaacttttggtggtagtggtggttctacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctatatcttcaaatgagcagcctgagagccgaggacacggccatatacttctgtgcgaaagaaggacctacgcttctaactcgcccccctctcaactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG1 100 100 [291] g163>a,A55>T(- - -)|a166>t,I56>F(+ - -)|a169>g,S57>G(+ + -)|a190>t|g191>c|c192>t|g261>a|g267>t|a275>g,N92>S(- - -)|g301>a,V101>I(+ - +)|t306>c|a308>t,Y103>F(- + -)| g163>a,A55>T(- - -)| a166>t,I56>F(+ - -)|a169>g,S57>G(+ + -)|a190>t|g191>c|c192>t| g261>a|g267>t|a275>g,N92>S(- - -)|g301>a,V101>I(+ - +)|t306>c|a308>t,Y103>F(- + -)| 320 225 213 12 6 6 2 3 1 1 0 0 3 0 1 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 19 5 3 2 1 0 1 0 0 0 2 0 1 0 0 0 117 114 108 6 3 3 1 2 0 1 0 0 1 0 0 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcagctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaact tttggtggtagtggtggttctaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctatatcttcaaatgagcagcctgagagccgaggacacggccatatacttctgt gcgaaagaaggacctacgcttctaactcgcccccctctcaac AKEGPTLLTRPPLN IGHV3-23 IGHD4-23 IGHJ4 ggattcacctttagcagctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaact tttggtggtagtggtggttctaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctatatcttcaaatgagcagcctgagagccgaggacacggccatatacttctgt gcgaaagaaggacctacgcttctaactcgcccccctctcaac IGG1 12 213 7 5 4 6 3 6 3 3 4 2 JY8QFUQ01AMDQC 11637 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 983 94.12 208/221 nt 93.67 207/221 nt Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-10*01 F 2 8 8 17 8.8.17 [2.17.38.11] CAIEHTLPSGQGLGIEYFW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 72 of V-REGION: 'A' (1 nucleotides at position 126 to 126 in the user submitted sequence), (cause frameshift); cagcctctggattcaactttaacagctttgccatgagctgggtccgccaggttccagggatggggctggagtgggtctcagccattagtggtagtggcgggagcacattctacgcagactccgtgAaagggccggttcaccatctccagagacaactccaacaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccttatattattgtgcgatcgagcatacccttccgtccggacagggattagggattgaatatttctggggccagggaaccctggtcatcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [304] c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|a110>t,Y37>F(- + -)|c134>t,A45>V(+ - +)|a143>t,K48>M(- + -)|t165>c|t186>c|t189>g|a197>t,Y66>F(- + -)|t246>c|g252>c,K84>N(+ - -)|g301>t,V101>L(+ - +)|c309>t| c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|a110>t,Y37>F(- + -)| c134>t,A45>V(+ - +)|a143>t,K48>M(- + -)|t165>c| t186>c|t189>g| a197>t,Y66>F(- + -)|t246>c|g252>c,K84>N(+ - -)|g301>t,V101>L(+ - +)|c309>t| a317>t,K106>I(- + -)|a318>c,K106>I(- + -)| 316 (320) 225 (229) 212 (214) 13 (15) 5 8 (10) 0 1 2 3 0 (1) 1 3 (4) 0 1 0 1 1 78 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 1 1 0 0 0 0 0 51 51 48 3 1 2 0 0 1 1 0 0 1 0 0 0 0 0 30 24 22 2 2 0 0 0 0 1 0 0 0 0 0 0 0 1 117 114 109 5 2 3 0 0 1 1 0 0 1 0 1 0 1 0 4 (8) 4 4 (6) 0 (2) 0 0 (2) 0 0 0 0 0 (1) 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cagcctct ggattcaactttaacagctttgcc atgagctgggtccgccaggttccagggatggggctggagtgggtctcagcc attagtggtagtggcgggagcaca ttctacgcagactccgtgaagggccggttcaccatctccagagacaactccaacaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccttatattattgt gcgatcgagcatacccttccgtccggacagggattagggattgaatatttc AIEHTLPSGQGLGIEYF IGHV3-23 IGHD3-10 IGHJ4 ggattcaactttaacagctttgcc atgagctgggtccgccaggttccagggatggggctggagtgggtctcagcc attagtggtagtggcgggagcaca ttctacgcagactccgtgaagggccggttcaccatctccagagacaactccaacaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccttatattattgt gcgatcgagcatacccttccgtccggacagggattagggattgaatatttc IGA2 13 213 6 7 3 6 3 7 3 2 5 3 JY8QFUQ01AMI66 20317 productive (see comment) Homsap IGHV4-4*07 F 935 93.02 200/215 nt 92.56 199/215 nt Homsap IGHJ3*02 F 214 92 46/50 nt Homsap IGHD3-22*01 F 2 8 7 19 8.7.19 [1.17.38.11] CARGKDYYDSTGSLEDGLDIW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'C' (1 nucleotides at position 13 to 13 in the user submitted sequence), (cause frameshift); tctctggtggctCccctcagtacttactactggaactgggtccggcagcccgccgggaagggactggagtggattgggcgtaaatatcccagtggggacaccaactataatccctccctcaaaagtcgagtcaccatgtcactagacacgtccaagaaccagttctccctgaggctgacctctgtgaccgccgcggacacggccgtgtattactgtgcgagagggaaggattactatgatagtactggttccctcgaggatggtcttgatatctggggccaagggacaaaggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [304] a88>c,I30>L(+ + +)|g107>c,S36>T(+ - +)|g119>a,S40>N(- - -)|a124>g,I42>V(+ - +)|t167>a,I56>K(- + -)|c168>a,I56>K(- + -)|a172>c,T58>P(+ + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c201>t|c204>t|g216>a|g238>c,V80>L(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)| a88>c,I30>L(+ + +)|g107>c,S36>T(+ - +)| g119>a,S40>N(- - -)|a124>g,I42>V(+ - +)| t167>a,I56>K(- + -)|c168>a,I56>K(- + -)|a172>c,T58>P(+ + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| c201>t|c204>t|g216>a|g238>c,V80>L(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)| a320>g| 319 (320) 222 (223) 207 15 (16) 3 (4) 12 3 (4) 3 2 0 2 1 0 1 3 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 1 0 0 0 1 0 0 0 51 51 49 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 30 21 16 5 0 5 1 1 0 0 1 1 0 1 0 0 0 0 117 114 108 6 3 3 1 1 2 0 0 0 0 0 2 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccctcagtacttactac tggaactgggtccggcagcccgccgggaagggactggagtggattgggcgt aaatatcccagtggggacacc aactataatccctccctcaaaagtcgagtcaccatgtcactagacacgtccaagaaccagttctccctgaggctgacctctgtgaccgccgcggacacggccgtgtattactgt gcgagagggaaggattactatgatagtactggttccctcgaggatggtcttgatatc ARGKDYYDSTGSLEDGLDI IGHV4-4 IGHD3-22 IGHJ3 ggtggctccctcagtacttactac tggaactgggtccggcagcccgccgggaagggactggagtggattgggcgt aaatatcccagtggggacacc aactataatccctccctcaaaagtcgagtcaccatgtcactagacacgtccaagaaccagttctccctgaggctgacctctgtgaccgccgcggacacggccgtgtattactgt gcgagagggaaggattactatgatagtactggttccctcgaggatggtcttgatatc IGA1 15 210 8 7 5 9 3 6 3 0 5 7 JY8QFUQ01AMIC0 16047 productive Homsap IGHV4-4*02 F, or Homsap IGHV4-4*03 F or Homsap IGHV4-4*04 F or Homsap IGHV4-4*05 F 972 94.47 205/217 nt NA Homsap IGHJ1*01 F (see comment) 152 76.92 40/52 nt Homsap IGHD3-22*01 F 3 9 7 11 9.7.11 [1.17.38.11] CAGLLTTFGLQSW in-frame + Other possibilities: Homsap_IGHJ4*02 and Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctggtggctccatcagcagtagtaactggtggacttgggtccgccagcccccagggaaggggctggagtggattggggaaatctatcatggtggaaacacctactacaacccgtccctcaagagtcgagtcaccatatcactagacaagtccaagaaccaattctctctgaacctgaactctgtgaccgccgcggacacggccatatattactgtgcgggacttcttacgacttttggactgcagagctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctgtccagggcttcttccccc NA IGA1 100 100 [281] g119>c,S40>T(+ - +)|a175>g,S59>G(+ + -)|g189>a|g191>a,S64>N(- - -)|a196>t,N66>Y(- - -)|g238>c,V80>L(+ - +)|g258>a|c264>t|g270>c,K90>N(+ - -)|g275>a,S92>N(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|a316>g,R106>G(- - -)| g119>c,S40>T(+ - +)| a175>g,S59>G(+ + -)|g189>a|g191>a,S64>N(- - -)| a196>t,N66>Y(- - -)|g238>c,V80>L(+ - +)|g258>a|c264>t|g270>c,K90>N(+ - -)|g275>a,S92>N(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| a316>g,R106>G(- - -)|g319>c|a320>t| 318 (320) 223 (225) 210 13 (15) 3 (5) 10 2 6 1 0 0 0 1 (2) 0 3 (4) 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 27 27 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 21 18 3 1 2 1 2 0 0 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 4 1 0 0 0 1 0 2 0 0 0 6 (8) 6 5 1 (3) 0 (2) 1 1 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtggctccatcagcagtagtaactgg tggacttgggtccgccagcccccagggaaggggctggagtggattggggaa atctatcatggtggaaacacc tactacaacccgtccctcaagagtcgagtcaccatatcactagacaagtccaagaaccaattctctctgaacctgaactctgtgaccgccgcggacacggccatatattactgt gcgggacttcttacgacttttggactgcagagc AGLLTTFGLQS IGHV4-4 IGHD3-22 IGHJ1 ggtggctccatcagcagtagtaactgg tggacttgggtccgccagcccccagggaaggggctggagtggattggggaa atctatcatggtggaaacacc tactacaacccgtccctcaagagtcgagtcaccatatcactagacaagtccaagaaccaattctctctgaacctgaactctgtgaccgccgcggacacggccatatattactgt gcgggacttcttacgacttttggactgcagagc IGA1 12 213 8 4 7 10 1 2 2 1 7 2 JY8QFUQ01AMK5G 11158 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 963 94.01 204/217 nt NA Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD2-8*02 F 2 8 8 20 8.8.20 [1.17.38.11] CARDLWLCSGGECYHRGGFENW in-frame + ctctgggttcaccttcagtaactcctggatgcactgggtccgccaagctccagggaaggggccggagtgggtctcacgtattaatagtgatgggagtaatacaatctacgcggactccgtgaagggccgactcaccatctccagagacaacgccaaaaacacggtgtatctgcaattgaacagtctgagagccgaggacacggctgtgtactactgtgcaagagatctgtggctttgtagtggtggtgaatgctatcatcggggtggttttgagaactggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 79 95 [308, 307] a81>g|g107>a,S36>N(- - -)|a110>c,Y37>S(+ - -)|t149>c,L50>P(- - -)|t152>a,V51>E(- + -)|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)|g197>t,S66>I(- - -)|t226>c,F76>L(+ - -)|g252>a|c259>g,L87>V(+ - +)|a271>t,M91>L(+ + -)|t306>c| a81>g|g107>a,S36>N(- - -)|a110>c,Y37>S(+ - -)| t149>c,L50>P(- - -)|t152>a,V51>E(- + -)| g191>a,S64>N(- - -)|c192>t,S64>N(- - -)| g197>t,S66>I(- - -)|t226>c,F76>L(+ - -)|g252>a|c259>g,L87>V(+ - +)|a271>t,M91>L(+ + -)|t306>c| 320 225 212 13 3 10 1 3 1 3 1 0 1 1 0 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 1 1 0 0 1 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 0 1 0 0 0 1 0 0 0 0 30 24 22 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 117 114 108 6 2 4 0 1 0 2 0 0 1 0 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcaccttcagtaactcctgg atgcactgggtccgccaagctccagggaaggggccggagtgggtctcacgt attaatagtgatgggagtaataca atctacgcggactccgtgaagggccgactcaccatctccagagacaacgccaaaaacacggtgtatctgcaattgaacagtctgagagccgaggacacggctgtgtactactgt gcaagagatctgtggctttgtagtggtggtgaatgctatcatcggggtggttttgagaac ARDLWLCSGGECYHRGGFEN IGHV3-74 IGHD2-8 IGHJ3 gggttcaccttcagtaactcctgg atgcactgggtccgccaagctccagggaaggggccggagtgggtctcacgt attaatagtgatgggagtaataca atctacgcggactccgtgaagggccgactcaccatctccagagacaacgccaaaaacacggtgtatctgcaattgaacagtctgagagccgaggacacggctgtgtactactgt gcaagagatctgtggctttgtagtggtggtgaatgctatcatcggggtggttttgagaac IGA2 13 213 8 5 4 6 4 7 2 1 6 4 JY8QFUQ01AMLKN 17612 productive Homsap IGHV3-30*08 F, or Homsap IGHV3-30*16 F 923 91.74 200/218 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD5-12*01 F 3 8 8 14 8.8.14 [1.17.38.11] CARGKAGHTHYGLDVW in-frame + cctctgcattcaccttcagtagttatgctatgcactgggtccgccaggccccaggcaaggggctagagtgggtggcagttatatcaaatgatggaaattataacgactatgcagactccgtgaaggggcgattcaccatttccagagacaactccaagaacacgctatttctacaaatgaatagcctgagagttgaggacacggctgtctataactgtgcgagaggaaaggctggccatacccactacggattggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [291] c108>t|t135>c|t172>a,Y58>N(- - -)|g188>a,S63>N(- - -)|a190>t,N64>Y(- - -)|a195>c,K65>N(+ - -)|t196>g,Y66>D(- - -)|c201>t|c222>g|c234>t|t246>c|g261>a|a263>t,Y88>F(- + -)|g267>a|c276>t|c287>t,A96>V(+ - +)|g303>c|t307>a,Y103>N(- - -)| c108>t| t135>c| t172>a,Y58>N(- - -)|g188>a,S63>N(- - -)|a190>t,N64>Y(- - -)|a195>c,K65>N(+ - -)| t196>g,Y66>D(- - -)|c201>t|c222>g|c234>t|t246>c|g261>a|a263>t,Y88>F(- + -)|g267>a|c276>t|c287>t,A96>V(+ - +)|g303>c|t307>a,Y103>N(- - -)| 318 224 206 18 10 8 0 3 5 2 1 0 2 2 1 1 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 30 24 20 4 0 4 0 1 0 0 1 0 1 1 0 0 0 0 117 114 102 12 8 4 0 2 4 1 0 0 1 1 1 1 0 1 6 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct gcattcaccttcagtagttatgct atgcactgggtccgccaggccccaggcaaggggctagagtgggtggcagtt atatcaaatgatggaaattataac gactatgcagactccgtgaaggggcgattcaccatttccagagacaactccaagaacacgctatttctacaaatgaatagcctgagagttgaggacacggctgtctataactgt gcgagaggaaaggctggccatacccactacggattggacgtc ARGKAGHTHYGLDV IGHV3-30 IGHD5-12 IGHJ6 gcattcaccttcagtagttatgct atgcactgggtccgccaggccccaggcaaggggctagagtgggtggcagtt atatcaaatgatggaaattataac gactatgcagactccgtgaaggggcgattcaccatttccagagacaactccaagaacacgctatttctacaaatgaatagcctgagagttgaggacacggctgtctataactgt gcgagaggaaaggctggccatacccactacggattggacgtc IGA1 18 213 10 8 8 10 2 8 9 1 4 4 JY8QFUQ01AMPZY 17760 productive Homsap IGHV1-69*06 F, or Homsap IGHV1-69*10 F or Homsap IGHV1-69*14 F 804 86.45 185/214 nt NA Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 232 96 48/50 nt Homsap IGHD2-8*02 F 3 8 8 22 8.8.22 [0.17.38.11] CARGGKSPRRDIIQGVTVDALDLW in-frame + tggagacaccttcagtagccaagccatcagctgggtgcgacaggcccctggacaaggacttgagtggatgggcgggatcatccctatttttgatatgacgaggtacccacagaggttcgagggcagaatcacgattaccgcggacacgtccacgaccacactttacatggaactgagcagcctgaggtctgaggacacggccgtgtatttttgtgcgagaggcgggaagagtcctcgtcgtgatattatacagggggtgactgttgatgctcttgatctctggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [312, 311] g83>a,G28>D(- - -)|c105>t|t109>c,Y37>Q(- - -)|t111>a,Y37>Q(- - -)|t114>c|g147>a|a162>c|c177>t|g188>a,G63>D(- - -)|c191>t,T64>M(- - -)|a192>g,T64>M(- - -)|g193>a,A65>T(- - -)|a195>g,A65>T(- - -)|a197>g,N66>R(+ - -)|c198>g,N66>R(+ - -)|g202>c,A68>P(- - -)|a209>g,K70>R(+ + +)|c214>g,Q72>E(+ + -)|g226>a,V76>I(+ - +)|a245>c,K82>T(- - -)|a246>g,K82>T(- - -)|g254>c,S85>T(+ - +)|g259>c,A87>L(+ - +)|c260>t,A87>L(+ - +)|c261>t,A87>L(+ - +)|g270>a|a285>g|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g83>a,G28>D(- - -)|c105>t|t109>c,Y37>Q(- - -)|t111>a,Y37>Q(- - -)|t114>c| g147>a|a162>c| c177>t|g188>a,G63>D(- - -)|c191>t,T64>M(- - -)|a192>g,T64>M(- - -)|g193>a,A65>T(- - -)|a195>g,A65>T(- - -)| a197>g,N66>R(+ - -)|c198>g,N66>R(+ - -)|g202>c,A68>P(- - -)|a209>g,K70>R(+ + +)|c214>g,Q72>E(+ + -)|g226>a,V76>I(+ - +)|a245>c,K82>T(- - -)|a246>g,K82>T(- - -)|g254>c,S85>T(+ - +)|g259>c,A87>L(+ - +)|c260>t,A87>L(+ - +)|c261>t,A87>L(+ - +)|g270>a|a285>g|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| a320>g| 319 (320) 221 (222) 192 29 (30) 7 (8) 22 6 (7) 6 6 2 2 0 1 1 3 2 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 1 1 2 0 0 0 1 0 0 0 0 51 51 49 2 2 0 0 1 0 0 1 0 0 0 0 0 0 0 30 24 18 6 1 5 2 2 2 0 0 0 0 0 0 0 0 0 117 114 98 16 2 14 4 2 3 0 1 0 1 0 3 2 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|tt,184-185(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggca,82-85(CDR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agca,192-195(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggagacaccttcagtagccaagcc atcagctgggtgcgacaggcccctggacaaggacttgagtggatgggcggg atcatccctatttttgatatgacg aggtacccacagaggttcgagggcagaatcacgattaccgcggacacgtccacgaccacactttacatggaactgagcagcctgaggtctgaggacacggccgtgtatttttgt gcgagaggcgggaagagtcctcgtcgtgatattatacagggggtgactgttgatgctcttgatctc ARGGKSPRRDIIQGVTVDALDL IGHV1-69 IGHD2-8 IGHJ3 ggagacaccttcagtagccaagcc atcagctgggtgcgacaggcccctggacaaggacttgagtggatgggcggg atcatccctatttttgatatgacg aggtacccacagaggttcgagggcagaatcacgattaccgcggacacgtccacgaccacactttacatggaactgagcagcctgaggtctgaggacacggccgtgtatttttgt gcgagaggcgggaagagtcctcgtcgtgatattatacagggggtgactgttgatgctcttgatctc IGA1 29 213 20 9 12 17 8 12 4 3 14 8 JY8QFUQ01AMTHS 22068 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 938 91.86 203/221 nt NA Homsap IGHJ5*02 F 183 84.31 43/51 nt Homsap IGHD3-10*01 F 2 10 7 16 10.7.16 [1.17.38.11] CVRPDNYFGSGSAWMDPW in-frame + tctctggtggctccatcagcagtgataatttctactggggctggatccgccagcccccagggaagggactgcagtggattgggactttctattatagagggagtatctattacaacccgtccctcaagagtcgagtcaccatatccgtggacacatccaagaaccagttctccctgaggctgacctctgtgaccgccgcagacacggctgtctattattgtgtgagacccgataattactttggttcggggagtgcctggatggacccctggggccacggaaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccctgaaccggtgacggtgtcgtggaactca NA IGG2 100 100 [301] a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)|g147>a|g151>c,E51>Q(+ + -)|g164>c,S55>T(+ - +)|a166>t,I56>F(+ - -)|t177>a,S59>R(- - -)|c192>t|c194>t,T65>I(- - -)|c198>t|a240>g|g246>a|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|g303>c|c309>t|c314>t,A105>V(+ - +)| a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)| g147>a|g151>c,E51>Q(+ + -)|g164>c,S55>T(+ - +)| a166>t,I56>F(+ - -)|t177>a,S59>R(- - -)|c192>t|c194>t,T65>I(- - -)| c198>t|a240>g|g246>a|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|g303>c|c309>t| c314>t,A105>V(+ - +)|a320>c| 319 (320) 228 (229) 209 19 (20) 7 (8) 12 3 4 5 0 0 (1) 0 2 1 4 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 0 4 1 2 0 0 0 0 1 0 0 0 0 0 51 51 48 3 1 2 0 1 0 0 0 0 0 0 2 0 0 0 30 21 17 4 1 3 0 0 2 0 0 0 1 1 0 0 0 0 117 114 107 7 5 2 2 1 2 0 0 0 0 0 2 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcagtgataatttctac tggggctggatccgccagcccccagggaagggactgcagtggattgggact ttctattatagagggagtatc tattacaacccgtccctcaagagtcgagtcaccatatccgtggacacatccaagaaccagttctccctgaggctgacctctgtgaccgccgcagacacggctgtctattattgt gtgagacccgataattactttggttcggggagtgcctggatggacccc VRPDNYFGSGSAWMDP IGHV4-39 IGHD3-10 IGHJ5 ggtggctccatcagcagtgataatttctac tggggctggatccgccagcccccagggaagggactgcagtggattgggact ttctattatagagggagtatc tattacaacccgtccctcaagagtcgagtcaccatatccgtggacacatccaagaaccagttctccctgaggctgacctctgtgaccgccgcagacacggctgtctattattgt gtgagacccgataattactttggttcggggagtgcctggatggacccc IGG2 18 216 11 7 8 12 3 6 6 1 4 7 JY8QFUQ01AMUT4 12589 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 860 88.53 193/218 nt 88.07 192/218 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-15*01 F 3 8 8 14 8.8.14 [1.17.38.11] CAKEEGATGVYYVDHW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 57 of V-REGION: 'A' (1 nucleotides at position 86 to 86 in the user submitted sequence), (cause frameshift); cctctggattcacctttaccacctccgccatggcctgggtccgccaggttccagggaaggggctggagtgggtctcaactattagAccctagtggtgagagaacctactacgcagagtccgtgaggggccgcttcaccatctccagagacaattccgagaacacgttgtatctacaactgaacaacctgagagtcgaggacacggccatatattactgtgcgaaggaggaaggtgctactggagtctattatgttgaccactggggccagggaacccgggtcaccgtctcctcatcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [293] g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|g163>a,A55>T(- - -)|t171>c|g172>c,G58>P(+ - -)|g173>c,G58>P(+ - -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)|a195>c|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|g225>c|a250>g,K84>E(+ - -)|c259>t|g267>a|a271>c,M91>L(+ + -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|a318>g| g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|g163>a,A55>T(- - -)| t171>c|g172>c,G58>P(+ - -)|g173>c,G58>P(+ - -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)|a195>c| c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|g225>c|a250>g,K84>E(+ - -)|c259>t|g267>a|a271>c,M91>L(+ + -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)| a318>g| 320 226 200 26 6 20 4 5 3 2 3 1 0 0 6 1 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 1 1 0 0 0 2 0 0 0 51 51 47 4 0 4 1 1 1 0 0 0 0 0 1 0 0 0 30 24 17 7 2 5 0 1 0 1 1 1 0 0 2 0 0 1 117 114 104 10 3 7 2 3 2 0 1 0 0 0 1 1 0 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttaccacctccgcc atggcctgggtccgccaggttccagggaaggggctggagtgggtctcaact attagccctagtggtgagagaacc tactacgcagagtccgtgaggggccgcttcaccatctccagagacaattccgagaacacgttgtatctacaactgaacaacctgagagtcgaggacacggccatatattactgt gcgaaggaggaaggtgctactggagtctattatgttgaccac AKEEGATGVYYVDH IGHV3-23 IGHD2-15 IGHJ4 ggattcacctttaccacctccgcc atggcctgggtccgccaggttccagggaaggggctggagtgggtctcaact attagccctagtggtgagagaacc tactacgcagagtccgtgaggggccgcttcaccatctccagagacaattccgagaacacgttgtatctacaactgaacaacctgagagtcgaggacacggccatatattactgt gcgaaggaggaaggtgctactggagtctattatgttgaccac IGG2 25 213 13 12 8 16 5 9 3 2 11 9 JY8QFUQ01AMX59 9183 productive (see comment) Homsap IGHV3-33*03 F 936 92.63 201/217 nt 91.71 199/217 nt Homsap IGHJ3*01 F (see comment) 160 80 40/50 nt Homsap IGHD6-19*01 F 2 8 7 15 8.7.15 [1.17.38.11] CARDGGAVAGLDVVDVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT at codon 28 of V-REGION: 'G' (1 nucleotides at position 11 to 11 in the user submitted sequence), (cause frameshift); in CDR2-IMGT, from codon 64 of V-REGION: 1 nucleotides (from position 101 in the user submitted sequence), (cause frameshift) cgtctggattGcattttcaacagctatgccatgcactgggtccgccaggctccaggcaagggcctggagtgggtggcagttatatggtttgatggaagtaaaaatattacgcagactcagtgaagggccgatccaccgtctccagagacaactccaagaacacgttgtatctgcaaatgaacagcctgagagccggggacacggccgtgtattattgtgcgagagatgggggagcagtggctggtttggacgttgtggacgtctggggccaagggaccacggtcaccgtcgcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [295] c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g113>c,G38>A(- + -)|g147>c|a173>t,Y58>F(- + -)|c198>t|t201>c|c210>a|t227>c,F76>S(- - -)|a232>g,I78>V(+ - +)|c259>t|a290>g,E97>G(- - -)|t300>c|c309>t|a317>g,K106>R(+ + +)| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g113>c,G38>A(- + -)| g147>c| a173>t,Y58>F(- + -)| c198>t|t201>c|c210>a|t227>c,F76>S(- - -)|a232>g,I78>V(+ - +)|c259>t|a290>g,E97>G(- - -)|t300>c|c309>t| a317>g,K106>R(+ + +)| 320 225 208 17 7 10 3 1 5 4 0 1 1 0 2 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 1 2 1 0 0 0 0 1 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 23 22 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 117 114 105 9 6 3 2 0 3 3 0 1 0 0 0 0 0 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|aact,244-247(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cgtct ggattcattttcaacagctatgcc atgcactgggtccgccaggctccaggcaagggcctggagtgggtggcagtt atatggtttgatggaagtaaaaa tattacgcagactcagtgaagggccgatccaccgtctccagagacaactccaagaacacgttgtatctgcaaatgaacagcctgagagccggggacacggccgtgtattattgt gcgagagatgggggagcagtggctggtttggacgttgtggacgtc ARDGGAVAGLDVVDV IGHV3-33 IGHD6-19 IGHJ3 ggattcattttcaacagctatgcc atgcactgggtccgccaggctccaggcaagggcctggagtgggtggcagtt atatggtttgatggaagtaaaaa tattacgcagactcagtgaagggccgatccaccgtctccagagacaactccaagaacacgttgtatctgcaaatgaacagcctgagagccggggacacggccgtgtattattgt gcgagagatgggggagcagtggctggtttggacgttgtggacgtc IGG1 16 212 12 4 6 9 6 7 7 0 3 6 JY8QFUQ01AMXZ6 15583 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 867 89.72 192/214 nt NA Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F 78 62.5 30/48 nt Homsap IGHD4-23*01 ORF 3 8 7 12 8.7.12 [1.17.38.11] CASSVFSPGGFDYW in-frame + tcgggggttcaccgtcagtagcaagtacatgacctgggtccgccaggctccggggaagggactggagtctgtctctgttttttatagcggtgatcaaacatactacgcagactccgtgaggggccgattcaccatctccatagacaattccaagaacacactgtatcttcaaatgaacggcctgcgagccgaggacacggccgtgtattattgtgcgagctccgtgtttagtcccggaggctttgactactggggccagggaaccctgtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccctaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 71 95 [280, 282] c75>t|t76>c,S26>R(- - -)|c77>g,S26>R(- - -)|t78>g,S26>R(- - -)|c111>g,N37>K(+ - -)|g119>c,S40>T(+ - +)|a138>g|g147>a|g155>c,W52>S(- - -)|g156>t,W52>S(- - -)|a162>t|a166>t,I56>F(+ - -)|g188>a,G63>D(- - -)|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>a,S64>Q(- - -)|a215>g,K72>R(+ + +)|g239>t,R80>I(- + -)|g258>a|a277>g,S93>G(+ + -)|a283>c|c309>t| c75>t|t76>c,S26>R(- - -)|c77>g,S26>R(- - -)|t78>g,S26>R(- - -)| c111>g,N37>K(+ - -)| g119>c,S40>T(+ - +)|a138>g|g147>a|g155>c,W52>S(- - -)|g156>t,W52>S(- - -)|a162>t| a166>t,I56>F(+ - -)|g188>a,G63>D(- - -)|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>a,S64>Q(- - -)| a215>g,K72>R(+ + +)|g239>t,R80>I(- + -)|g258>a|a277>g,S93>G(+ + -)|a283>c|c309>t| a318>c,R106>S(- - -)|g319>t|a320>c| 317 (320) 219 (222) 197 22 (25) 7 (9) 15 (16) 3 4 2 1 2 (4) 1 2 0 2 2 2 (3) 1 78 4 0 4 1 3 0 0 1 1 0 0 0 0 0 1 0 1 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 45 6 3 3 1 1 0 0 0 0 1 0 2 0 1 0 30 21 16 5 0 5 0 2 0 0 1 1 1 0 0 0 0 0 117 114 108 6 3 3 2 1 1 0 1 0 0 0 0 0 1 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 0 0 0 0 (2) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tcgg gggttcaccgtcagtagcaagtac atgacctgggtccgccaggctccggggaagggactggagtctgtctctgtt ttttatagcggtgatcaaaca tactacgcagactccgtgaggggccgattcaccatctccatagacaattccaagaacacactgtatcttcaaatgaacggcctgcgagccgaggacacggccgtgtattattgt gcgagctccgtgtttagtcccggaggctttgactac ASSVFSPGGFDY IGHV3-53 IGHD4-23 IGHJ4 gggttcaccgtcagtagcaagtac atgacctgggtccgccaggctccggggaagggactggagtctgtctctgtt ttttatagcggtgatcaaaca tactacgcagactccgtgaggggccgattcaccatctccatagacaattccaagaacacactgtatcttcaaatgaacggcctgcgagccgaggacacggccgtgtattattgt gcgagctccgtgtttagtcccggaggctttgactac IGA2 18 210 8 10 5 11 3 7 6 0 6 6 JY8QFUQ01AMZ6W 11234 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 854 88.84 191/215 nt 88.37 190/215 nt Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD3-10*01 F 2 8 7 16 8.7.16 [1.17.38.11] CARVDFYGSSKYKGFDPW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 57 of V-REGION: 'C' (1 nucleotides at position 84 to 84 in the user submitted sequence), (cause frameshift); tctctggtaactccatcacagattattactggagctggctccggcagtctccagggaagggactggagtggattggaaatgtcCttttacagtgggagagccaactacaacccctccctcaagagtcgagtcaccatatcagtagccacgtccaggaaccagttctccctgaagctcaggtctgtgaccactgcggacacggccatatattattgtgcgagagttgatttctatggttcgagtaaatataaggggttcgacccctggggccagggagccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [296] g82>a,G28>N(- - -)|g83>a,G28>N(- - -)|g104>c,S35>T(+ - +)|t105>a,S35>T(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c111>t|a124>c,I42>L(+ + +)|c133>t,P45>S(+ - -)|c135>t,P45>S(+ - -)|g162>a|t163>a,Y55>N(- - -)|a166>g,I56>V(+ - +)|a170>t,Y57>F(- + -)|c192>a,S64>R(- - -)|a193>g,T65>A(- - -)|a242>c,D81>A(- - -)|a251>g,K84>R(+ + +)|g273>c|c276>g,S92>R(- - -)|g286>a,A96>T(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| g82>a,G28>N(- - -)|g83>a,G28>N(- - -)|g104>c,S35>T(+ - +)|t105>a,S35>T(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c111>t| a124>c,I42>L(+ + +)|c133>t,P45>S(+ - -)|c135>t,P45>S(+ - -)|g162>a|t163>a,Y55>N(- - -)| a166>g,I56>V(+ - +)|a170>t,Y57>F(- + -)|c192>a,S64>R(- - -)|a193>g,T65>A(- - -)| a242>c,D81>A(- - -)|a251>g,K84>R(+ + +)|g273>c|c276>g,S92>R(- - -)|g286>a,A96>T(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| a320>t| 319 (320) 222 (223) 198 24 (25) 4 (5) 20 4 7 4 0 2 1 1 (2) 2 2 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 1 6 1 3 1 0 0 0 0 1 1 0 0 0 51 51 46 5 1 4 0 1 2 0 1 0 0 1 0 0 0 0 30 21 17 4 0 4 2 0 0 0 0 1 1 0 0 0 0 0 117 114 106 8 2 6 1 3 1 0 1 0 0 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtaactccatcacagattattac tggagctggctccggcagtctccagggaagggactggagtggattggaaat gtcttttacagtgggagagcc aactacaacccctccctcaagagtcgagtcaccatatcagtagccacgtccaggaaccagttctccctgaagctcaggtctgtgaccactgcggacacggccatatattattgt gcgagagttgatttctatggttcgagtaaatataaggggttcgacccc ARVDFYGSSKYKGFDP IGHV4-59 IGHD3-10 IGHJ5 ggtaactccatcacagattattac tggagctggctccggcagtctccagggaagggactggagtggattggaaat gtcttttacagtgggagagcc aactacaacccctccctcaagagtcgagtcaccatatcagtagccacgtccaggaaccagttctccctgaagctcaggtctgtgaccactgcggacacggccatatattattgt gcgagagttgatttctatggttcgagtaaatataaggggttcgacccc IGG1 24 210 15 9 11 15 4 9 3 1 10 10 JY8QFUQ01AN3IA 8104 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 1008 96.31 209/217 nt NA Homsap IGHJ3*01 F 241 98 49/50 nt Homsap IGHD2-15*01 F 3 8 8 17 8.8.17 [1.17.38.11] CARDTLEVVSNMDDAFDVW in-frame + ctctggattcaccttcagtcgttatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatacattagtaggagtagtactgacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacggcaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtatttctgtgcgagagatactctagaggtagtgtctaatatggatgatgcttttgatgtctggggccaagggacaatggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [299] a106>c,S36>R(- - -)|c108>t,S36>R(- - -)|c164>a,S55>Y(+ - -)|t174>g,S58>R(- - -)|g188>c,S63>T(+ - +)|t190>g,Y64>D(- - -)|c248>g,A83>G(- + -)|a308>t,Y103>F(- + -)| a106>c,S36>R(- - -)|c108>t,S36>R(- - -)| c164>a,S55>Y(+ - -)| t174>g,S58>R(- - -)|g188>c,S63>T(+ - +)|t190>g,Y64>D(- - -)| c248>g,A83>G(- + -)|a308>t,Y103>F(- + -)| 320 225 217 8 0 8 0 0 1 0 1 1 1 0 1 1 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 1 0 1 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 30 24 21 3 0 3 0 0 0 0 0 0 0 0 1 0 0 2 117 114 112 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtcgttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatac attagtaggagtagtactgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacggcaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtatttctgt gcgagagatactctagaggtagtgtctaatatggatgatgcttttgatgtc ARDTLEVVSNMDDAFDV IGHV3-21 IGHD2-15 IGHJ3 ggattcaccttcagtcgttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatac attagtaggagtagtactgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacggcaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtatttctgt gcgagagatactctagaggtagtgtctaatatggatgatgcttttgatgtc IGA1 8 213 1 7 1 4 0 4 0 0 3 5 JY8QFUQ01AN5X2 11065 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 930 92.99 199/214 nt 92.06 197/214 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 3 8 7 12 8.7.12 [1.16.38.11] CARDTGQWKDFDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 49 of V-REGION: 1 nucleotides (from position 59 in the user submitted sequence), (cause frameshift)in CDR2-IMGT, from codon 58 of V-REGION: 1 nucleotides (from position 85 in the user submitted sequence), (cause frameshift) tctggattcatcttcagtagctactggatgcactgggtccgccaagctccagggaagggctggtgtgggtgtcacgtagtaatacggggggactgacacagcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgtgcaagagatacggggcagtggaaggactttgactactggggccagggaaccctggtcaccgtctccttagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcncatgcctggtccagggcttcttccc NA IGA2 79 89 [281] c86>t,T29>I(- - -)|c159>g|t167>g,I56>S(- - -)|t174>c|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| c86>t,T29>I(- - -)| c159>g| t167>g,I56>S(- - -)|t174>c|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| 320 222 207 15 4 11 3 1 1 1 1 0 0 0 2 3 1 2 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 50 49 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 30 23 16 7 1 6 2 1 0 1 0 0 0 0 1 0 0 2 117 114 108 6 2 4 1 0 0 0 1 0 0 0 1 2 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaagggctggtgtgggtgtcacgt agtaatacggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac ARDTGQWKDFDY IGHV3-74 IGHD6-19 IGHJ4 ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaagggctggtgtgggtgtcacgt agtaatacggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac IGA2 15 211 6 9 2 8 4 7 3 1 4 7 JY8QFUQ01AN6Q8 10379 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F or Homsap IGHV3-15*06 F 921 90.58 202/223 nt NA Homsap IGHJ5*02 F 183 84.31 43/51 nt Homsap IGHD2-21*01 F 2 8 10 8 8.10.8 [1.17.38.11] CTSNSWLDRW in-frame - ctctggattctctttcagtaatgcctggatgaattgggtccgccaggctccagggaaggggctggaatgggttggccatattaaaagggaaattgatggtgggataacaaactacgctgcacccgtgaagggcagattctccatcgcaagagatgattcaaagaatatgatgtatttgcaaatgaacagcctgaacaccgaggacacaggcgtgtattactgtacctcaaattcgtggctcgaccgctggggccagggaacccccgtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [279] a85>t,T29>S(+ - +)|c108>t|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g153>a|g164>a,R55>H(- - +)|c174>g,S58>R(- - -)|a175>g,K59>E(+ - -)|c179>t,T60>I(- - -)|c191>t,T64>I(- - -)|g196>a,D66>N(+ + -)|a216>g|a229>t,T77>S(+ - +)|t235>g,S79>A(- + -)|a252>g|c255>t|c257>t,T86>M(- - -)|c259>a,L87>M(+ + -)|c265>t|a285>c,K95>N(+ - -)|c299>g,A100>G(- + -)|a316>t,T106>S(+ - +)| a85>t,T29>S(+ - +)|c108>t| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g153>a|g164>a,R55>H(- - +)| c174>g,S58>R(- - -)|a175>g,K59>E(+ - -)|c179>t,T60>I(- - -)|c191>t,T64>I(- - -)| g196>a,D66>N(+ + -)|a216>g|a229>t,T77>S(+ - +)|t235>g,S79>A(- + -)|a252>g|c255>t|c257>t,T86>M(- - -)|c259>a,L87>M(+ + -)|c265>t|a285>c,K95>N(+ - -)|c299>g,A100>G(- + -)| a316>t,T106>S(+ - +)|g319>a| 318 (320) 229 (231) 207 (208) 22 (23) 6 (7) 16 3 4 (5) 7 0 1 1 3 0 0 2 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 1 0 0 0 1 0 0 0 0 0 51 51 47 4 1 3 0 3 1 0 0 0 0 0 0 0 0 0 30 30 26 4 0 4 1 0 2 0 0 0 0 0 0 1 0 0 117 114 103 11 4 7 2 1 3 0 1 1 1 0 0 1 0 1 6 (8) 6 5 (6) 1 (2) 0 (1) 1 0 0 (1) 0 0 0 0 1 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| ctct ggattctctttcagtaatgcctgg atgaattgggtccgccaggctccagggaaggggctggaatgggttggccat attaaaagggaaattgatggtgggataaca aactacgctgcacccgtgaagggcagattctccatcgcaagagatgattcaaagaatatgatgtatttgcaaatgaacagcctgaacaccgaggacacaggcgtgtattactgt acctcaaattcgtggctcgaccgc TSNSWLDR IGHV3-15 IGHD2-21 IGHJ5 ggattctctttcagtaatgcctgg atgaattgggtccgccaggctccagggaaggggctggaatgggttggccat attaaaagggaaattgatggtgggataaca aactacgctgcacccgtgaagggcagattctccatcgcaagagatgattcaaagaatatgatgtatttgcaaatgaacagcctgaacaccgaggacacaggcgtgtattactgt acctcaaattcgtggctcgaccgc IGG2 21 219 14 7 11 14 3 7 5 1 10 5 JY8QFUQ01AN8DC 21425 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 936 92.63 201/217 nt 92.17 200/217 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD6-19*01 F 2 8 8 14 8.8.14 [1.17.38.11] CAKEIAVSGEGALDFW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'A' (1 nucleotides at position 13 to 13 in the user submitted sequence), (cause frameshift); ctctggattcacAgtttagcaactatgccatgagctgggtccgccaggctccagggaaggggccggagtgggtctcagctattactggtcttgatggtagaacattctacgcagactccgtgaagggccgtttctccatctccagagacaattccaagaacacgttgtatttgggaatgaacagcctgagagccgaggacacggccgtatattattgtgcgaaagagatagcagtgagtggtgagggggcgttggacttctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [291] c87>g|g107>a,S36>N(- - -)|t149>c,L50>P(- - -)|g170>c,S57>T(+ - +)|a175>c,S59>L(- - -)|g176>t,S59>L(- - -)|g185>a,G62>D(- - -)|c192>a,S64>R(- - -)|a197>t,Y66>F(- + -)|g225>t|a229>t,T77>S(+ - +)|c259>t|c265>t|c268>g,Q90>G(- - -)|a269>g,Q90>G(- - -)|c309>t| c87>g|g107>a,S36>N(- - -)| t149>c,L50>P(- - -)| g170>c,S57>T(+ - +)|a175>c,S59>L(- - -)|g176>t,S59>L(- - -)|g185>a,G62>D(- - -)|c192>a,S64>R(- - -)| a197>t,Y66>F(- + -)|g225>t|a229>t,T77>S(+ - +)|c259>t|c265>t|c268>g,Q90>G(- - -)|a269>g,Q90>G(- - -)|c309>t| 320 225 209 16 5 11 1 2 3 1 1 1 2 0 1 2 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 1 0 0 0 0 0 0 0 1 0 0 51 51 50 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 30 24 19 5 0 5 0 1 0 0 1 1 0 0 1 0 1 0 117 114 106 8 4 4 1 0 3 0 0 0 2 0 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgtttagcaactatgcc atgagctgggtccgccaggctccagggaaggggccggagtgggtctcagct attactggtcttgatggtagaaca ttctacgcagactccgtgaagggccgtttctccatctccagagacaattccaagaacacgttgtatttgggaatgaacagcctgagagccgaggacacggccgtatattattgt gcgaaagagatagcagtgagtggtgagggggcgttggacttc AKEIAVSGEGALDF IGHV3-23 IGHD6-19 IGHJ4 ggattcacgtttagcaactatgcc atgagctgggtccgccaggctccagggaaggggccggagtgggtctcagct attactggtcttgatggtagaaca ttctacgcagactccgtgaagggccgtttctccatctccagagacaattccaagaacacgttgtatttgggaatgaacagcctgagagccgaggacacggccgtatattattgt gcgaaagagatagcagtgagtggtgagggggcgttggacttc IGA1 16 213 7 9 5 11 2 5 4 1 5 6 JY8QFUQ01AN937 3606 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F (see comment) 776 83.71 185/221 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 2 10 7 20 10.7.20 [1.17.38.11] CARQSEPANNFNDPSRYFFDTW in-frame + Low V-REGION identity (83,71% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tctcccgtgtctccatttccattaatgattactactggggctggatccgccagcccccaggaaagccgctggagtggattgggactgtccattcccttgggtacaattacaacaacccgtccctcaagagtcgactcaccatttccgcagacacgtccaggaatcagatctccctgaaactgacgtctgtgaccgccgcagacacggctgtctatttctgtgcgagacagagcgagcccgccaataacttcaatgacccaagtcgttatttctttgacacttggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [312] t78>c|g79>c,G27>R(- - -)|g83>t,G28>V(- - -)|c90>t|a91>t|g92>c|g101>t,S34>I(- - -)|g104>a,S35>N(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g141>a|g145>c,G49>P(+ - -)|g146>c,G49>P(+ - -)|g164>c,S55>T(+ - +)|a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|a173>c,Y58>S(+ - -)|t174>c,Y58>S(+ - -)|a175>c,S59>L(- - -)|g176>t,S59>L(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|c194>a,T65>N(- + -)|c195>t,T65>N(- + -)|t199>a,Y67>N(- - -)|g226>c,V76>L(+ - +)|a234>t|t239>c,V80>A(+ - +)|a251>g,K84>R(+ + +)|c255>t|t259>a,F87>I(+ - -)|g270>a|g275>c,S92>T(+ - +)|c276>g,S92>T(+ - +)|g303>c|a308>t,Y103>F(- + -)| t78>c| g79>c,G27>R(- - -)|g83>t,G28>V(- - -)|c90>t|a91>t|g92>c|g101>t,S34>I(- - -)|g104>a,S35>N(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g141>a|g145>c,G49>P(+ - -)|g146>c,G49>P(+ - -)|g164>c,S55>T(+ - +)| a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|a173>c,Y58>S(+ - -)|t174>c,Y58>S(+ - -)|a175>c,S59>L(- - -)|g176>t,S59>L(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|c194>a,T65>N(- + -)|c195>t,T65>N(- + -)| t199>a,Y67>N(- - -)|g226>c,V76>L(+ - +)|a234>t|t239>c,V80>A(+ - +)|a251>g,K84>R(+ + +)|c255>t|t259>a,F87>I(+ - -)|g270>a|g275>c,S92>T(+ - +)|c276>g,S92>T(+ - +)|g303>c|a308>t,Y103>F(- + -)| 320 229 193 36 9 27 3 5 3 4 2 1 4 2 8 1 3 0 78 5 4 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 30 21 9 3 6 1 2 1 0 0 0 1 0 2 0 2 0 51 51 47 4 1 3 0 1 0 0 0 0 0 0 3 0 0 0 30 21 11 10 0 10 1 1 1 2 2 1 1 0 0 0 1 0 117 114 102 12 4 8 1 1 1 1 0 0 2 2 3 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctcc cgtgtctccatttccattaatgattactac tggggctggatccgccagcccccaggaaagccgctggagtggattgggact gtccattcccttgggtacaat tacaacaacccgtccctcaagagtcgactcaccatttccgcagacacgtccaggaatcagatctccctgaaactgacgtctgtgaccgccgcagacacggctgtctatttctgt gcgagacagagcgagcccgccaataacttcaatgacccaagtcgttatttctttgacact ARQSEPANNFNDPSRYFFDT IGHV4-39 IGHD3-22 IGHJ4 cgtgtctccatttccattaatgattactac tggggctggatccgccagcccccaggaaagccgctggagtggattgggact gtccattcccttgggtacaat tacaacaacccgtccctcaagagtcgactcaccatttccgcagacacgtccaggaatcagatctccctgaaactgacgtctgtgaccgccgcagacacggctgtctatttctgt gcgagacagagcgagcccgccaataacttcaatgacccaagtcgttatttctttgacact IGA2 35 216 14 21 8 21 6 14 5 3 11 16 JY8QFUQ01AND2L 18282 productive Homsap IGHV4-31*02 F, or Homsap IGHV4-31*03 F or Homsap IGHV4-31*04 F 929 91.4 202/221 nt NA Homsap IGHJ1*01 F 242 96.15 50/52 nt Homsap IGHD2-21*02 F 2 10 7 18 10.7.18 [1.17.38.11] CARSAYCGADCYSADYLQHW in-frame - tctctggtggctccgtcagcagtaggggttactactggaactggatccgccagttcccagggaagggcctggagtggattgggaacatcttttacagtgggggcacctacgacaacccgtccctcaggagtcgaatttctatatcattagacacgtctaagaaccaattctccctgaagttgacctctgtgaccgccgcggacacggccgtgtattactgtgcgagatcggcatactgtggtgctgactgctactcagctgattacctccagcactggggccagggcaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [306] a88>g,I30>V(+ - +)|g103>a,G35>R(- - -)|t105>g,G35>R(- - -)|g119>a,S40>N(- - -)|c133>t,H45>F(- - -)|a134>t,H45>F(- - -)|t163>a,Y55>N(- - -)|a170>t,Y57>F(- + -)|a190>g,S64>G(+ + -)|t199>g,Y67>D(- - -)|a215>g,K72>R(+ + +)|g226>a,V76>I(+ - +)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|g238>t,V80>L(+ - +)|g258>a|c271>t|g275>c,S92>T(+ - +)|t285>c| a88>g,I30>V(+ - +)|g103>a,G35>R(- - -)|t105>g,G35>R(- - -)| g119>a,S40>N(- - -)|c133>t,H45>F(- - -)|a134>t,H45>F(- - -)|t163>a,Y55>N(- - -)| a170>t,Y57>F(- + -)|a190>g,S64>G(+ + -)| t199>g,Y67>D(- - -)|a215>g,K72>R(+ + +)|g226>a,V76>I(+ - +)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|g238>t,V80>L(+ - +)|g258>a|c271>t|g275>c,S92>T(+ - +)|t285>c| g319>t|a320>c| 318 (320) 227 (229) 208 19 (21) 3 (5) 16 3 4 3 1 0 (1) 0 3 1 1 0 1 (2) 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 1 1 0 0 0 0 0 0 0 0 0 1 51 51 47 4 0 4 0 1 1 0 0 0 1 1 0 0 0 0 30 21 19 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 117 114 104 10 3 7 1 2 2 1 0 0 1 0 1 0 1 1 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,228-229(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,69-70(FR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,227-228(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|agca,131-134(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agtt,225-228(FR3)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,69-72(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|tacc,228-231(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tgcc,285-288(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcagcagtaggggttactac tggaactggatccgccagttcccagggaagggcctggagtggattgggaac atcttttacagtgggggcacc tacgacaacccgtccctcaggagtcgaatttctatatcattagacacgtctaagaaccaattctccctgaagttgacctctgtgaccgccgcggacacggccgtgtattactgt gcgagatcggcatactgtggtgctgactgctactcagctgattacctccagcac ARSAYCGADCYSADYLQH IGHV4-31 IGHD2-21 IGHJ1 ggtggctccgtcagcagtaggggttactac tggaactggatccgccagttcccagggaagggcctggagtggattgggaac atcttttacagtgggggcacc tacgacaacccgtccctcaggagtcgaatttctatatcattagacacgtctaagaaccaattctccctgaagttgacctctgtgaccgccgcggacacggccgtgtattactgt gcgagatcggcatactgtggtgctgactgctactcagctgattacctccagcac IGA1 19 216 11 8 7 9 4 10 3 0 11 5 JY8QFUQ01ANGEF 13561 productive Homsap IGHV4-4*07 F 817 87.32 186/213 nt NA Homsap IGHJ3*02 F 160 80 40/50 nt Homsap IGHD3-22*01 F 2 8 7 11 8.7.11 [1.17.38.11] CAGRNGNLAFNIW in-frame + tctggtggttcgatcgtcagttactactggagttggatccggcagtccgccgggaagggactggagtggattgggcgcatctactccaatggagatacgaactacaatccctccctcaagggtcgagtcaccatgtcagtagacccttccaacaaccagttctccctgaaattgacttctgtgaccgccgcggacacggccatatattactgtgcgggaagaaatggaaatttggccttcaatatttggggccaagggacaaatgtcaccgtctcctcagcatcccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [276] c84>t|c87>g|a103>g,S35>V(- - -)|g104>t,S35>V(- - -)|t105>c,S35>V(- - -)|c120>t|c133>t,P45>S(+ - -)|t165>c|t171>c|a172>t,T58>S(+ - +)|g176>a,S59>N(- - -)|g189>a|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c192>t,S64>D(- - -)|c195>g|c204>t|a220>g,S74>G(+ + -)|a244>c,T82>P(+ + -)|g246>t,T82>P(+ + -)|g252>c,K84>N(+ - -)|g270>a|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|a316>g,R106>G(- - -)| c84>t|c87>g|a103>g,S35>V(- - -)|g104>t,S35>V(- - -)|t105>c,S35>V(- - -)| c120>t|c133>t,P45>S(+ - -)|t165>c| t171>c|a172>t,T58>S(+ - +)|g176>a,S59>N(- - -)|g189>a|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c192>t,S64>D(- - -)|c195>g| c204>t|a220>g,S74>G(+ + -)|a244>c,T82>P(+ + -)|g246>t,T82>P(+ + -)|g252>c,K84>N(+ - -)|g270>a|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| a316>g,R106>G(- - -)|g319>a|a320>g| 318 (320) 219 (221) 191 28 (30) 10 (12) 18 4 (5) 6 (7) 7 3 1 0 1 0 2 2 2 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 1 0 1 1 0 0 0 0 0 1 1 0 51 51 48 3 2 1 0 0 2 1 0 0 0 0 0 0 0 0 30 21 13 8 3 5 1 3 1 1 0 0 1 0 0 1 0 0 117 114 103 11 3 8 1 3 3 0 1 0 0 0 2 0 1 0 6 (8) 6 5 1 (3) 0 (2) 1 1 (2) 0 (1) 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tct ggtggttcgatcgtcagttactac tggagttggatccggcagtccgccgggaagggactggagtggattgggcgc atctactccaatggagatacg aactacaatccctccctcaagggtcgagtcaccatgtcagtagacccttccaacaaccagttctccctgaaattgacttctgtgaccgccgcggacacggccatatattactgt gcgggaagaaatggaaatttggccttcaatatt AGRNGNLAFNI IGHV4-4 IGHD3-22 IGHJ3 ggtggttcgatcgtcagttactac tggagttggatccggcagtccgccgggaagggactggagtggattgggcgc atctactccaatggagatacg aactacaatccctccctcaagggtcgagtcaccatgtcagtagacccttccaacaaccagttctccctgaaattgacttctgtgaccgccgcggacacggccatatattactgt gcgggaagaaatggaaatttggccttcaatatt IGA1 27 210 19 8 13 19 6 8 5 5 9 8 JY8QFUQ01ANI1H 13786 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 828 87.1 189/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [1.17.38.11] CTRDIGGRYSYW in-frame - ctctggattcagcctcattgactttagaatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattgatagtgttggtcaaaacatatactacagagactcagtgcggggccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctcagagtcgaggacacggctatatattactgtacaagagacatcggtgggcgctactcctactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [278, 277] c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|g104>t,S35>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>t,Y37>F(- + -)|c114>a,S38>R(- - -)|a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|a184>g,S62>G(+ + -)|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>a,S63>Q(- - -)|t190>a,Y64>N(- - -)|g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|a214>c,K72>R(+ + +)|a215>g,K72>R(+ + +)|t256>a,S86>R(- - -)|c257>g,S86>R(- - -)|a258>g,S86>R(- - -)|c279>t|g282>c|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|g313>a,A105>T(- - -)|g315>a,A105>T(- - -)| c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|g104>t,S35>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>t,Y37>F(- + -)|c114>a,S38>R(- - -)| a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|a184>g,S62>G(+ + -)|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>a,S63>Q(- - -)|t190>a,Y64>N(- - -)| g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|a214>c,K72>R(+ + +)|a215>g,K72>R(+ + +)|t256>a,S86>R(- - -)|c257>g,S86>R(- - -)|a258>g,S86>R(- - -)|c279>t|g282>c|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g313>a,A105>T(- - -)|g315>a,A105>T(- - -)| 320 225 195 30 2 28 6 8 2 1 2 1 1 3 1 3 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 0 7 1 1 0 1 0 1 1 0 0 1 1 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 15 9 0 9 3 2 0 0 1 0 0 2 0 0 1 0 117 114 102 12 2 10 2 3 2 0 1 0 0 1 1 2 0 0 8 8 6 2 0 2 0 2 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagcctcattgactttaga atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attgatagtgttggtcaaaacata tactacagagactcagtgcggggccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctcagagtcgaggacacggctatatattactgt acaagagacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-21 IGHD1-26 IGHJ4 ggattcagcctcattgactttaga atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attgatagtgttggtcaaaacata tactacagagactcagtgcggggccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctcagagtcgaggacacggctatatattactgt acaagagacatcggtgggcgctactcctac IGA2 28 213 15 13 8 15 7 13 2 0 10 16 JY8QFUQ01ANJ9X 4704 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 910 90.87 199/219 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-3*01 F 2 8 8 10 8.8.10 [2.17.38.11] CASDLGGQKSYW in-frame - ccgtcaggattcaccttcagtagatactggatgcactgggtccgccaagctccagggaaggggccggtgtgggtctcacgtactaatgaagatggcacccacataaattacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtatttgcaaatgaacagtctgagagccgaggacacggctgtctattactgtgcaagcgatttgggtggtcagaaaagttactggggccagggagtcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccca NA IGA1 100 100 [280] g73>c,A25>P(- - -)|c75>g,A25>P(- - -)|t78>a|c108>a,S36>R(- - -)|t149>c,L50>P(- - -)|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>c|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|c194>t,T65>I(- - -)|g197>a,S66>N(- - -)|c198>t,S66>N(- - -)|c257>a,T86>K(- - -)|c265>t|g303>c|a318>c,R106>S(- - -)| g73>c,A25>P(- - -)|c75>g,A25>P(- - -)|t78>a| c108>a,S36>R(- - -)| t149>c,L50>P(- - -)| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>c|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|c194>t,T65>I(- - -)| g197>a,S66>N(- - -)|c198>t,S66>N(- - -)|c257>a,T86>K(- - -)|c265>t|g303>c| a318>c,R106>S(- - -)| 320 227 206 21 4 17 1 3 3 3 2 2 0 2 4 1 0 0 78 6 3 3 1 2 0 0 0 0 0 0 0 1 1 1 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 30 24 14 10 1 9 1 2 1 2 1 0 0 1 2 0 0 0 117 114 109 5 2 3 0 1 2 0 0 1 0 0 1 0 0 0 8 8 7 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ccgtca ggattcaccttcagtagatactgg atgcactgggtccgccaagctccagggaaggggccggtgtgggtctcacgt actaatgaagatggcacccacata aattacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtatttgcaaatgaacagtctgagagccgaggacacggctgtctattactgt gcaagcgatttgggtggtcagaaaagttac ASDLGGQKSY IGHV3-74 IGHD3-3 IGHJ4 ggattcaccttcagtagatactgg atgcactgggtccgccaagctccagggaaggggccggtgtgggtctcacgt actaatgaagatggcacccacata aattacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtatttgcaaatgaacagtctgagagccgaggacacggctgtctattactgt gcaagcgatttgggtggtcagaaaagttac IGA1 17 213 10 7 6 11 4 6 2 1 4 10 JY8QFUQ01ANKJV 5253 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 874 89.04 195/219 nt 88.58 194/219 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD1-1*01 F 2 8 8 9 8.8.9 [2.17.38.11] CARRQLALAYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 59 to 59 in the user submitted sequence), (cause frameshift); catctgggatacacctttatcacctactggatcgcctgggtgcgccaaatgcccgggaAaaggcctggagttgatgggagtcatctatcctggtgactctgagaccagatacagcccgtccttccaaggccacatcaccctctcagtcgacaagtccatcgataccgcctacctggagtggagcagcctgaaggcctcggacaccgccatgtacttctgtgcaagaagacaactggcccttgcctactggggccagggaacccaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [278] g73>c,G25>H(+ - -)|g74>a,G25>H(+ - -)|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)|g86>c,S29>T(+ - +)|c104>t,T35>I(- - -)|g107>c,S36>T(+ - +)|g119>c,G40>A(- + -)|g132>a|g155>t,W52>L(+ - -)|g162>a|a163>g,I55>V(+ - +)|t192>g,D64>E(+ - +)|g225>c,Q75>H(- + -)|g226>a,V76>I(+ - +)|a232>c,I78>L(+ + +)|c239>t,A80>V(+ - +)|a253>g,S85>D(- - -)|g254>a,S85>D(- - -)|c255>t,S85>D(- - -)|c268>g,Q90>E(+ + -)|t306>c|a308>t,Y103>F(- + -)|g315>a| g73>c,G25>H(+ - -)|g74>a,G25>H(+ - -)|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)| g86>c,S29>T(+ - +)|c104>t,T35>I(- - -)|g107>c,S36>T(+ - +)| g119>c,G40>A(- + -)|g132>a|g155>t,W52>L(+ - -)|g162>a|a163>g,I55>V(+ - +)| t192>g,D64>E(+ - +)| g225>c,Q75>H(- + -)|g226>a,V76>I(+ - +)|a232>c,I78>L(+ + +)|c239>t,A80>V(+ - +)|a253>g,S85>D(- - -)|g254>a,S85>D(- - -)|c255>t,S85>D(- - -)|c268>g,Q90>E(+ + -)|t306>c|a308>t,Y103>F(- + -)| g315>a|c319>a|a320>g| 318 (320) 225 (227) 200 25 (27) 4 (6) 21 2 (3) 6 4 2 1 0 (1) 1 0 5 1 1 2 78 6 1 5 0 5 0 1 1 1 0 0 0 0 1 0 0 1 36 24 21 3 0 3 0 0 1 0 0 0 0 0 2 0 0 0 51 51 46 5 2 3 1 2 0 0 0 0 0 0 1 0 1 0 30 24 23 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 117 114 104 10 1 9 1 2 2 1 1 0 1 0 1 1 0 0 6 (8) 6 5 1 (3) 1 (3) 0 0 (1) 1 0 0 0 0 (1) 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| catctg ggatacacctttatcacctactgg atcgcctgggtgcgccaaatgcccgggaaaggcctggagttgatgggagtc atctatcctggtgactctgagacc agatacagcccgtccttccaaggccacatcaccctctcagtcgacaagtccatcgataccgcctacctggagtggagcagcctgaaggcctcggacaccgccatgtacttctgt gcaagaagacaactggcccttgcctac ARRQLALAY IGHV5-51 IGHD1-1 IGHJ4 ggatacacctttatcacctactgg atcgcctgggtgcgccaaatgcccgggaaaggcctggagttgatgggagtc atctatcctggtgactctgagacc agatacagcccgtccttccaaggccacatcaccctctcagtcgacaagtccatcgataccgcctacctggagtggagcagcctgaaggcctcggacaccgccatgtacttctgt gcaagaagacaactggcccttgcctac IGA2 19 213 10 9 7 13 3 6 3 0 12 4 JY8QFUQ01ANLMX 17132 productive Homsap IGHV3-49*03 F, or Homsap IGHV3-49*05 F 962 92.41 207/224 nt NA Homsap IGHJ3*01 F 232 96 48/50 nt Homsap IGHD1-26*01 F 3 8 10 14 8.10.14 [1.17.38.11] CTRDLSGSYSGAFDVW in-frame + gatctggattcacctttggtgactttgctatgagctggtttcgccaggctccagggaaggggctggagtggctaggtttcattagaagcaaaatttatggtgggacaccagaatacgccgcgtctgtgaaaggcagatgtcccatctcaagagatgattccaaaaacatcgcctatctgcaaataaacggcctgaaaaccgaggacacagccatgtatttctgtaccagagatctcagtgggagctactccggtgcttttgatgtctggggccaagggacaatggtcaccgtctcttcagcatccccggaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [298] c74>g|t75>a|t108>c|a110>t,Y37>F(- + -)|c126>t|g157>c,V53>L(+ - +)|g178>a,A60>I(+ - +)|c179>t,A60>I(+ - +)|a193>c,T65>P(+ + -)|t227>g,F76>C(+ - -)|c228>t,F76>C(+ - -)|a229>c,T77>P(+ + -)|g254>a,S85>N(- - -)|g273>a,M91>I(+ + -)|a277>g,S93>G(+ + -)|g301>a,V101>M(+ - -)|a308>t,Y103>F(- + -)|t315>c| c74>g|t75>a| t108>c|a110>t,Y37>F(- + -)| c126>t|g157>c,V53>L(+ - +)| g178>a,A60>I(+ - +)|c179>t,A60>I(+ - +)|a193>c,T65>P(+ + -)| t227>g,F76>C(+ - -)|c228>t,F76>C(+ - -)|a229>c,T77>P(+ + -)|g254>a,S85>N(- - -)|g273>a,M91>I(+ + -)|a277>g,S93>G(+ + -)|g301>a,V101>M(+ - -)|a308>t,Y103>F(- + -)| t315>c| 320 232 214 18 5 13 1 4 3 2 2 0 2 1 1 1 0 1 78 5 3 2 2 0 0 0 0 0 0 0 0 1 0 1 0 0 36 24 22 2 1 1 0 0 0 1 0 0 1 0 0 0 0 0 51 51 49 2 1 1 0 0 1 0 0 0 0 0 1 0 0 0 30 30 27 3 0 3 0 1 1 0 1 0 0 0 0 0 0 0 117 114 106 8 0 8 1 3 1 0 1 0 1 0 0 0 0 1 8 8 7 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,158-159(FR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,181-182(CDR2)|aa,192-193(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|ta,69-70(FR1)|tt,75-76(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,124-125(FR2)|ta,158-159(FR2)|tt,162-163(FR2)|tt,167-168(CDR2)|tt,180-181(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agct,72-75(FR1)|agct,118-121(FR2)|ggtt,122-125(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggta,156-159(FR2)|ggtt,160-163(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|ggca,220-223(FR3)|agca,253-256(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,72-75(FR1)|tgct,111-114(CDR1)|agct,118-121(FR2)|agct,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| gatct ggattcacctttggtgactttgct atgagctggtttcgccaggctccagggaaggggctggagtggctaggtttc attagaagcaaaatttatggtgggacacca gaatacgccgcgtctgtgaaaggcagatgtcccatctcaagagatgattccaaaaacatcgcctatctgcaaataaacggcctgaaaaccgaggacacagccatgtatttctgt accagagatctcagtgggagctactccggtgcttttgatgtc TRDLSGSYSGAFDV IGHV3-49 IGHD1-26 IGHJ3 ggattcacctttggtgactttgct atgagctggtttcgccaggctccagggaaggggctggagtggctaggtttc attagaagcaaaatttatggtgggacacca gaatacgccgcgtctgtgaaaggcagatgtcccatctcaagagatgattccaaaaacatcgcctatctgcaaataaacggcctgaaaaccgaggacacagccatgtatttctgt accagagatctcagtgggagctactccggtgcttttgatgtc IGA1 15 219 9 6 7 8 2 7 1 1 9 4 JY8QFUQ01ANO71 19774 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 915 90.91 200/220 nt 90.45 199/220 nt Homsap IGHJ4*02 F 141 77.08 37/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [2.17.38.11] CTRDIGGRYSYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 47 of V-REGION: 'G' (1 nucleotides at position 57 to 57 in the user submitted sequence), (cause frameshift); agcctctggattcgccttcagttggtattggatgcactgggtccgccaagctccagGgggagggactggtgtgggtctcacggactaatgaagatgggagtatcacaaactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtttctgcaaatgaacagtctcagagtcgaggacacggctgtttattactgtacaagagacatcggtgggcgctactcctactggggccagggagtcctggtcaccgtctccttagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [282] a85>g,T29>A(- - -)|a106>t,S36>W(- - -)|c108>g,S36>W(- - -)|c111>t|a142>g,K48>E(+ - -)|g147>a|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|a263>t,Y88>F(- + -)|g282>c|c287>t,A96>V(+ - +)|g303>t|g313>a,A105>T(- - -)| a85>g,T29>A(- - -)|a106>t,S36>W(- - -)|c108>g,S36>W(- - -)|c111>t| a142>g,K48>E(+ - -)|g147>a|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|a263>t,Y88>F(- + -)|g282>c|c287>t,A96>V(+ - +)|g303>t| g313>a,A105>T(- - -)| 320 228 207 21 5 16 4 5 2 1 0 0 2 1 1 2 2 1 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 1 0 0 0 1 0 0 1 0 0 51 51 48 3 2 1 1 1 0 0 0 0 0 0 0 0 0 1 30 24 19 5 0 5 1 1 0 1 0 0 0 1 0 0 1 0 117 114 106 8 2 6 1 2 1 0 0 0 1 0 1 1 1 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct ggattcgccttcagttggtattgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtttctgcaaatgaacagtctcagagtcgaggacacggctgtttattactgt acaagagacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcgccttcagttggtattgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtttctgcaaatgaacagtctcagagtcgaggacacggctgtttattactgt acaagagacatcggtgggcgctactcctac IGA1 20 213 11 9 6 11 5 9 4 1 7 8 JY8QFUQ01ANOOW 17136 productive Homsap IGHV4-39*07 F (see comment) 788 84.86 185/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-19*01 F 1 9 7 15 9.7.15 [1.17.38.11] CARAVLDNAWPIYYDFW in-frame - The submitted sequence and the closest germline V-GENE allele show different CDR1-IMGT amino acid lengths (9 AA in JY8QFUQ01ANOOW ; 10 AA in Homsap_IGHV4-39*07), and low V-REGION identity (84,86% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tgtctggtggctccatcaccacttggcattactggggctggatccgccagcccccaggggagggtctggaatggattggaaatgtctatcataatggcaatatcgcctatagcccgtcgcagaggagtcgaatcaccatatcagtagacacgtccaggaaccagttctccctgaagatgacctctgtgaccgccgcggacacggctgtctattactgtgcgagggctgttcttgacaatgcctggcctatatattatgacttttggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [295] c75>g|g92>c,S31>T(+ - +)|g104>c,S35>T(+ - +)|a106>t,S36>W(- - -)|t108>g,S36>W(- - -)|t109>c,Y37>H(+ - -)|c111>t,Y37>H(+ - -)|a142>g,K48>E(+ - -)|g147>t|g153>a|g162>a|g164>a,S55>N(- - -)|a166>g,I56>V(+ - +)|t172>c,Y58>H(+ - -)|g176>a,S59>N(- - -)|g189>c|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)|c194>t,T65>I(- - -)|t196>g,Y66>A(- - -)|a197>c,Y66>A(- - -)|c201>t|a203>g,N68>S(- - -)|c210>g|t212>a,L71>Q(- - -)|c213>g,L71>Q(- - -)|a215>g,K72>R(+ + +)|g226>a,V76>I(+ - +)|a251>g,K84>R(+ + +)|c271>a,L91>M(+ + -)|g275>c,S92>T(+ - +)|c300>t|g303>c| c75>g| g92>c,S31>T(+ - +)|g104>c,S35>T(+ - +)|a106>t,S36>W(- - -)|t108>g,S36>W(- - -)|t109>c,Y37>H(+ - -)|c111>t,Y37>H(+ - -)| a142>g,K48>E(+ - -)|g147>t|g153>a|g162>a|g164>a,S55>N(- - -)| a166>g,I56>V(+ - +)|t172>c,Y58>H(+ - -)|g176>a,S59>N(- - -)|g189>c|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)|c194>t,T65>I(- - -)| t196>g,Y66>A(- - -)|a197>c,Y66>A(- - -)|c201>t|a203>g,N68>S(- - -)|c210>g|t212>a,L71>Q(- - -)|c213>g,L71>Q(- - -)|a215>g,K72>R(+ + +)|g226>a,V76>I(+ - +)|a251>g,K84>R(+ + +)|c271>a,L91>M(+ + -)|g275>c,S92>T(+ - +)|c300>t|g303>c| a318>g|a320>c| 317 (320) 223 (226) 190 (191) 33 (35) 9 (11) 24 5 (6) 6 5 2 1 (2) 1 1 1 5 3 1 2 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 27 21 6 0 6 0 0 1 1 0 0 1 0 2 0 0 1 51 51 46 5 3 2 1 3 0 0 0 0 0 0 0 0 1 0 30 21 14 7 1 6 1 2 2 1 0 0 0 0 1 0 0 0 117 114 100 14 4 10 3 1 2 0 1 1 0 1 2 2 0 1 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tgtct ggtggctccatcaccacttggcattac tggggctggatccgccagcccccaggggagggtctggaatggattggaaat gtctatcataatggcaatatc gcctatagcccgtcgcagaggagtcgaatcaccatatcagtagacacgtccaggaaccagttctccctgaagatgacctctgtgaccgccgcggacacggctgtctattactgt gcgagggctgttcttgacaatgcctggcctatatattatgacttt ARAVLDNAWPIYYDF IGHV4-39 IGHD6-19 IGHJ4 ggtggctccatcaccacttggcattac tggggctggatccgccagcccccaggggagggtctggaatggattggaaat gtctatcataatggcaatatc gcctatagcccgtcgcagaggagtcgaatcaccatatcagtagacacgtccaggaaccagttctccctgaagatgacctctgtgaccgccgcggacacggctgtctattactgt gcgagggctgttcttgacaatgcctggcctatatattatgacttt IGG2 32 213 18 14 11 20 7 12 7 1 12 12 JY8QFUQ01ANR0Z 14641 productive Homsap IGHV1-18*01 F 885 90.65 194/214 nt NA Homsap IGHJ5*02 F 228 94.12 48/51 nt Homsap IGHD3-10*01 F 3 8 8 11 8.8.11 [0.17.38.11] CARVIRTNWLDPW in-frame + tggttacacctttactacctatggcgtcacctgggtgcgacaggcccctggacaagggcctgagtgggtgggatggatcagcgcttacaatggtaatacaaactctgcacagaagtttcaggacagagtcaccctgaccacagacacatccacgaacacagcctacatggaactgaggaacttgagatctgacgacacagccgtatattattgtgcgcgagtgattcggaccaactggctcgacccctggggccagggaacactggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [278] c105>t|g107>c,S36>T(+ - +)|t114>c|a115>g,I39>V(+ - +)|g119>c,S40>T(+ - +)|t149>c,L50>P(- - -)|a157>g,M53>V(+ - -)|c192>t|a200>c,Y67>S(+ - -)|c211>t,L71>F(+ - -)|c213>t,L71>F(+ - -)|g221>a,G74>D(- - -)|a232>c,M78>L(+ + -)|g254>a,S85>N(- - -)|g270>a|g278>a,S93>N(- - -)|c280>t|g297>a|g303>a|c309>t|a316>c| c105>t|g107>c,S36>T(+ - +)|t114>c| a115>g,I39>V(+ - +)|g119>c,S40>T(+ - +)|t149>c,L50>P(- - -)|a157>g,M53>V(+ - -)| c192>t| a200>c,Y67>S(+ - -)|c211>t,L71>F(+ - -)|c213>t,L71>F(+ - -)|g221>a,G74>D(- - -)|a232>c,M78>L(+ + -)|g254>a,S85>N(- - -)|g270>a|g278>a,S93>N(- - -)|c280>t|g297>a|g303>a|c309>t| a316>c|a320>t| 319 (320) 221 (222) 200 21 (22) 9 (10) 12 2 6 6 2 3 0 0 (1) 0 2 0 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 1 1 0 0 0 0 1 0 0 0 51 51 47 4 0 4 2 0 0 1 0 0 0 0 1 0 0 0 30 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 117 114 102 12 5 7 0 6 4 0 2 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 1 (2) 0 0 0 0 0 1 0 0 (1) 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|ta,175-176(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,5-6(FR1)|tt,75-76(FR1)|tt,81-82(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|tt,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,3-6(FR1)|agct,8-11(FR1)|agct,24-27(FR1)|ggct,72-75(FR1)|ggtt,79-82(CDR1)|agct,106-109(CDR1)|ggta,112-115(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)| agct,8-11(FR1)|agct,24-27(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|agct,209-212(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggttacacctttactacctatggc gtcacctgggtgcgacaggcccctggacaagggcctgagtgggtgggatgg atcagcgcttacaatggtaataca aactctgcacagaagtttcaggacagagtcaccctgaccacagacacatccacgaacacagcctacatggaactgaggaacttgagatctgacgacacagccgtatattattgt gcgcgagtgattcggaccaactggctcgacccc ARVIRTNWLDP IGHV1-18 IGHD3-10 IGHJ5 ggttacacctttactacctatggc gtcacctgggtgcgacaggcccctggacaagggcctgagtgggtgggatgg atcagcgcttacaatggtaataca aactctgcacagaagtttcaggacagagtcaccctgaccacagacacatccacgaacacagcctacatggaactgaggaacttgagatctgacgacacagccgtatattattgt gcgcgagtgattcggaccaactggctcgacccc IGA1 20 213 16 4 12 14 4 6 5 3 11 1 JY8QFUQ01ANSMV 14331 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 892 89.95 197/219 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD1-26*01 F 1 8 8 15 8.8.15 [2.17.38.11] CAKDQSCIVGPCGFDYW in-frame - gcctccgaattcatccttgacagttatgccatgagttgggtccgccaggccccagggaaggggctggagtgggtctcggctattagtggaagtggtgcaaccacatactacgcagactccgtgaagggccggttcgccatctccagagacaattccaagaacacgctatatctacaaatgaacaacctaggggccgaggacacggccgtttattactgtgcgaaagatcagtcctgtatagtgggaccttgtggttttgactactggggccagggagccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgt NA IGG1 100 100 [296] t78>c|g80>a,G27>E(- - -)|c86>t,T29>I(- - -)|t88>c,F30>L(+ - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c108>t|c120>t|t135>c|a162>g|t174>a|g188>c,G63>A(- + -)|t189>a,G63>A(- + -)|g191>c,S64>T(+ - +)|a229>g,T77>A(- - -)|g261>a|g267>a|g278>a,S93>N(- - -)|g282>a|a283>g,R95>G(- - -)|a285>g,R95>G(- - -)|a303>t| t78>c| g80>a,G27>E(- - -)|c86>t,T29>I(- - -)|t88>c,F30>L(+ - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c108>t| c120>t|t135>c|a162>g| t174>a|g188>c,G63>A(- + -)|t189>a,G63>A(- + -)|g191>c,S64>T(+ - +)| a229>g,T77>A(- - -)|g261>a|g267>a|g278>a,S93>N(- - -)|g282>a|a283>g,R95>G(- - -)|a285>g,R95>G(- - -)|a303>t| 320 227 205 22 10 12 5 6 3 3 0 0 1 2 2 0 0 0 78 6 5 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 18 6 1 5 1 2 2 1 0 0 0 0 0 0 0 0 51 51 48 3 3 0 1 0 1 1 0 0 0 0 0 0 0 0 30 24 20 4 1 3 0 0 0 0 0 0 0 2 2 0 0 0 117 114 106 8 4 4 3 4 0 0 0 0 1 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctcc gaattcatccttgacagttatgcc atgagttgggtccgccaggccccagggaaggggctggagtgggtctcggct attagtggaagtggtgcaaccaca tactacgcagactccgtgaagggccggttcgccatctccagagacaattccaagaacacgctatatctacaaatgaacaacctaggggccgaggacacggccgtttattactgt gcgaaagatcagtcctgtatagtgggaccttgtggttttgactac AKDQSCIVGPCGFDY IGHV3-23 IGHD1-26 IGHJ4 gaattcatccttgacagttatgcc atgagttgggtccgccaggccccagggaaggggctggagtgggtctcggct attagtggaagtggtgcaaccaca tactacgcagactccgtgaagggccggttcgccatctccagagacaattccaagaacacgctatatctacaaatgaacaacctaggggccgaggacacggccgtttattactgt gcgaaagatcagtcctgtatagtgggaccttgtggttttgactac IGG1 21 213 16 5 9 11 7 10 7 2 4 8 JY8QFUQ01ANV52 18826 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 963 94.01 204/217 nt NA Homsap IGHJ6*02 F 265 91.94 57/62 nt Homsap IGHD1-20*01 F 1 8 8 19 8.8.19 [1.17.38.11] CANASPRITGTYFYYYGMDVW in-frame + ctctggattcatcttcagtagctatgccatgaattgggtccgccagactccagggaaggggctggagtgggtctcagccattagtggtagtggtggtaacacatactacgcagactccgtgaagggccggttcaccgtctccagagacaattccaacaacacgctgtatctgcaattggacagcctgcgagccgaggacacggccgtatattactgtgcgaatgcctccccccgtataactggaacgtatttctactactacggtatggacgtctggggccaagggaccacggtcagcgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG2 100 100 [306] c86>t,T29>I(- - -)|t90>c|c105>t|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g133>a,A45>T(- - -)|t165>c|g191>a,S64>N(- - -)|a232>g,I78>V(+ - +)|g252>c,K84>N(+ - -)|a271>t,M91>L(+ + -)|a274>g,N92>D(+ + -)|a283>c| c86>t,T29>I(- - -)|t90>c|c105>t| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g133>a,A45>T(- - -)|t165>c| g191>a,S64>N(- - -)| a232>g,I78>V(+ - +)|g252>c,K84>N(+ - -)|a271>t,M91>L(+ + -)|a274>g,N92>D(+ + -)|a283>c| a318>t,K106>N(+ - -)|a320>c| 317 (320) 222 (225) 209 (210) 13 (15) 4 (5) 9 (10) 2 3 3 2 1 (2) 0 1 (2) 0 1 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 2 1 0 0 0 0 0 0 0 0 51 51 47 4 1 3 0 2 1 1 0 0 0 0 0 0 0 0 30 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 117 114 109 5 1 4 2 0 0 0 1 0 1 0 1 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagtagctatgcc atgaattgggtccgccagactccagggaaggggctggagtgggtctcagcc attagtggtagtggtggtaacaca tactacgcagactccgtgaagggccggttcaccgtctccagagacaattccaacaacacgctgtatctgcaattggacagcctgcgagccgaggacacggccgtatattactgt gcgaatgcctccccccgtataactggaacgtatttctactactacggtatggacgtc ANASPRITGTYFYYYGMDV IGHV3-23 IGHD1-20 IGHJ6 ggattcatcttcagtagctatgcc atgaattgggtccgccagactccagggaaggggctggagtgggtctcagcc attagtggtagtggtggtaacaca tactacgcagactccgtgaagggccggttcaccgtctccagagacaattccaacaacacgctgtatctgcaattggacagcctgcgagccgaggacacggccgtatattactgt gcgaatgcctccccccgtataactggaacgtatttctactactacggtatggacgtc IGG2 13 213 10 3 6 7 4 6 2 2 7 2 JY8QFUQ01ANVJX 4887 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 890 90.7 195/215 nt 90.23 194/215 nt Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-9*01 F 2 8 8 10 8.8.10 [1.17.37.11] CVSDLAGQKGFW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 88 of V-REGION: 1 nucleotides (from position 167 in the user submitted sequence), (cause frameshift) tcaggattcaccttcagtaggtactggatgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgtattaatgaagacggcagctacacagatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgttttgcaaatgaacagtctgagaggcgaggacacggctgtctattactgtgttagcgatttggctggtcagaaaggtttctggggccagggagtcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [276, 275] t78>a|c108>g,S36>R(- - -)|c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|c265>t|c287>g,A96>G(- + -)|g303>c| t78>a| c108>g,S36>R(- - -)| c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|c265>t|c287>g,A96>G(- + -)|g303>c| c314>t,A105>V(+ - +)|a315>t,A105>V(+ - +)|a318>c,R106>S(- - -)| 320 223 200 23 6 17 2 3 4 4 1 1 2 2 2 2 0 0 78 3 2 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 49 2 0 2 0 0 1 1 0 0 0 0 0 0 0 0 30 24 16 8 3 5 1 2 0 2 0 0 1 1 1 0 0 0 117 113 105 8 2 6 1 1 2 1 0 1 0 0 1 1 0 0 8 8 5 3 0 3 0 0 1 0 1 0 1 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tca ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagctacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgttttgcaaatgaacagtctgagaggcgaggacacggctgtctattactgt gttagcgatttggctggtcagaaaggtttc VSDLAGQKGF IGHV3-74 IGHD3-9 IGHJ4 ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagctacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgttttgcaaatgaacagtctgagaggcgaggacacggctgtctattactgt gttagcgatttggctggtcagaaaggtttc IGA2 19 212 12 7 6 11 6 8 2 3 8 6 JY8QFUQ01ANXAZ 13943 productive (see comment) Homsap IGHV1-3*01 F 885 90.65 194/214 nt 90.19 193/214 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-25*01 F 1 8 8 13 8.8.13 [0.17.38.11] CARDGYRSGNGADYW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 71 of V-REGION: 'A' (1 nucleotides at position 116 to 116 in the user submitted sequence), (cause frameshift); tggatacagcttcactaactacaatatccattgggtgcgccaggcccccggacaagggcttgagtgggtgggatggatcaacgctggcaatggcaatacaagatattcacagaagAttgcagggcagagtcaccatttccagggacacatccgcgagcattgccaacatggagttgagcagcctgagatatgaagacacggctgtatattattgtgcgagagacggctataggagtggcaacggggctgactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [285] c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|t111>c|g112>a,A38>N(- - -)|c113>a,A38>N(- - -)|g117>c,M39>I(+ + -)|a145>g,R49>G(- - -)|a157>g,M53>V(+ - -)|t189>c|c192>t|a197>g,K66>R(+ + +)|c213>g,F71>L(+ - -)|a235>t,T79>S(+ - +)|c257>t,T86>I(- - -)|a258>t,T86>I(- - -)|t262>a,Y88>N(- - -)|c271>t|c287>a,S96>Y(+ - -)|g303>a|c309>t| c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|t111>c|g112>a,A38>N(- - -)|c113>a,A38>N(- - -)| g117>c,M39>I(+ + -)|a145>g,R49>G(- - -)|a157>g,M53>V(+ - -)| t189>c|c192>t| a197>g,K66>R(+ + +)|c213>g,F71>L(+ - -)|a235>t,T79>S(+ - +)|c257>t,T86>I(- - -)|a258>t,T86>I(- - -)|t262>a,Y88>N(- - -)|c271>t|c287>a,S96>Y(+ - -)|g303>a|c309>t| 320 222 202 20 6 14 3 3 4 2 0 2 2 1 1 2 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 2 0 1 0 1 0 0 0 1 0 0 51 51 48 3 0 3 2 0 0 0 0 0 0 0 1 0 0 0 30 24 22 2 2 0 0 0 1 1 0 0 0 0 0 0 0 0 117 114 104 10 3 7 1 1 3 0 0 1 2 1 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|ta,262-263(FR3)|aa,290-291(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,11-12(FR1)|tt,62-63(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,120-121(FR2)|tt,149-150(FR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,201-202(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacagcttcactaactacaat atccattgggtgcgccaggcccccggacaagggcttgagtgggtgggatgg atcaacgctggcaatggcaataca agatattcacagaagttgcagggcagagtcaccatttccagggacacatccgcgagcattgccaacatggagttgagcagcctgagatatgaagacacggctgtatattattgt gcgagagacggctataggagtggcaacggggctgactat ARDGYRSGNGADY IGHV1-3 IGHD6-25 IGHJ4 ggatacagcttcactaactacaat atccattgggtgcgccaggcccccggacaagggcttgagtgggtgggatgg atcaacgctggcaatggcaataca agatattcacagaagttgcagggcagagtcaccatttccagggacacatccgcgagcattgccaacatggagttgagcagcctgagatatgaagacacggctgtatattattgt gcgagagacggctataggagtggcaacggggctgactat IGA1 20 213 12 8 7 12 5 8 3 3 10 4 JY8QFUQ01ANXRG 13199 productive Homsap IGHV3-74*03 F 905 90.83 198/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-13*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVRDSDSSASYFAHW in-frame + cctctggattcaccttcagtagctactggatgcactgggtccgccaaggtccaggggaggggctaatgtgggtctcacgaatcaatactgatgggagtaacacaatgtacgcggactccgtaaagggccggttcaccatttccagagacaatgccaagaatacggtgtttctgcaaatgaacagtctgaaagccgacgacacggctgtctattattgtgttagagattcagacagtagcgcctcgtacttcgcccactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [288] c134>g,A45>G(- + -)|a142>g,K48>E(+ - -)|g150>a|g151>a,V51>M(+ - -)|t165>a|t168>c|g173>c,S58>T(+ - +)|g191>a,S64>N(- - -)|c197>t,T66>M(- - -)|g213>a|a225>g|c234>t|c246>t|c255>t|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|g284>a,R95>K(+ + +)|g291>c,E97>D(+ - +)|g303>c|c309>t|c314>t,A105>V(+ - +)|a315>t,A105>V(+ - +)| c134>g,A45>G(- + -)|a142>g,K48>E(+ - -)|g150>a|g151>a,V51>M(+ - -)|t165>a| t168>c|g173>c,S58>T(+ - +)|g191>a,S64>N(- - -)| c197>t,T66>M(- - -)|g213>a|a225>g|c234>t|c246>t|c255>t|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|g284>a,R95>K(+ + +)|g291>c,E97>D(+ - +)|g303>c|c309>t| c314>t,A105>V(+ - +)|a315>t,A105>V(+ - +)| 320 226 204 22 10 12 2 5 6 1 0 0 2 1 3 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 46 5 2 3 1 2 0 0 0 0 0 1 0 1 0 0 30 24 21 3 1 2 0 1 0 1 0 0 0 0 1 0 0 0 117 114 102 12 7 5 1 2 5 0 0 0 1 0 2 1 0 0 8 8 6 2 0 2 0 0 1 0 0 0 1 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtagctactgg atgcactgggtccgccaaggtccaggggaggggctaatgtgggtctcacga atcaatactgatgggagtaacaca atgtacgcggactccgtaaagggccggttcaccatttccagagacaatgccaagaatacggtgtttctgcaaatgaacagtctgaaagccgacgacacggctgtctattattgt gttagagattcagacagtagcgcctcgtacttcgcccac VRDSDSSASYFAH IGHV3-74 IGHD6-13 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaaggtccaggggaggggctaatgtgggtctcacga atcaatactgatgggagtaacaca atgtacgcggactccgtaaagggccggttcaccatttccagagacaatgccaagaatacggtgtttctgcaaatgaacagtctgaaagccgacgacacggctgtctattattgt gttagagattcagacagtagcgcctcgtacttcgcccac IGA1 20 213 13 7 10 15 3 5 9 1 8 2 JY8QFUQ01AO0HA 16066 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 882 89.86 195/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [1.17.38.11] CTRDIGGRYSYW in-frame - ctccggattcacctttactaattattggatgcactgggtccgccaagctccaggggagggactggtgtgggtctcacggactaatgaagatgggagtatcacaaactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctcagagtcgaggacacggctatatattactgtacaagagacatcggtgggcgctactcctactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [278] t78>c|c90>t|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|c111>t|a142>g,K48>E(+ - -)|g147>a|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|g282>c|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|g313>a,A105>T(- - -)| t78>c| c90>t|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|c111>t| a142>g,K48>E(+ - -)|g147>a|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|g282>c|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g313>a,A105>T(- - -)| 320 225 202 23 6 17 3 8 4 2 0 0 0 1 2 1 1 1 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 1 3 0 0 0 0 0 1 0 0 0 51 51 48 3 2 1 1 1 0 0 0 0 0 0 0 0 0 1 30 24 19 5 0 5 1 1 0 1 0 0 0 1 0 0 1 0 117 114 106 8 1 7 1 4 1 0 0 0 0 0 1 1 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcacctttactaattattgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctcagagtcgaggacacggctatatattactgt acaagagacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttactaattattgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctcagagtcgaggacacggctatatattactgt acaagagacatcggtgggcgctactcctac IGA2 21 213 15 6 11 15 4 6 3 2 8 8 JY8QFUQ01AO14S 15581 productive Homsap IGHV3-7*01 F 1011 97.2 208/214 nt NA Homsap IGHJ1*01 F (see comment) 152 76.92 40/52 nt Homsap IGHD2-21*02 F 2 8 8 10 8.8.10 [0.17.38.11] CSNGYGDFPRRW in-frame - Other possibilities: Homsap_IGHJ4*02 and Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) tggattcaccttcaggagctattggatgagctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagcaagatggaggtgacaaattctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgttcgaatggttacggtgacttcccgcgtaggtggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [275, 274] t90>c|t105>g,S35>R(- - -)|a187>g,S63>G(+ + -)|g192>c,E64>D(+ - +)|a197>t,Y66>F(- + -)|g303>a|g313>t,A105>S(- + -)| t90>c|t105>g,S35>R(- - -)| a187>g,S63>G(+ + -)|g192>c,E64>D(+ - +)| a197>t,Y66>F(- + -)|g303>a| g313>t,A105>S(- + -)|g317>a,R106>N(+ - -)|a318>t,R106>N(+ - -)|a320>g| 316 (320) 218 (222) 211 (212) 7 (10) 2 (3) 5 (7) 1 (2) 1 (2) 0 1 0 0 1 (2) 0 1 0 1 1 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 0 1 0 0 0 0 0 0 0 1 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 117 114 112 2 1 1 0 1 0 0 0 0 1 0 0 0 0 0 4 (8) 4 3 (4) 1 (4) 0 (1) 1 (3) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t ggattcaccttcaggagctattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaggtgacaaa ttctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt tcgaatggttacggtgacttcccgcgtagg SNGYGDFPRR IGHV3-7 IGHD2-21 IGHJ1 ggattcaccttcaggagctattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaggtgacaaa ttctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt tcgaatggttacggtgacttcccgcgtagg IGA2 6 213 3 3 1 2 2 4 1 1 1 3 JY8QFUQ01AO38F 20886 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 911 90.5 200/221 nt 90.05 199/221 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 2 10 7 12 10.7.12 [1.17.38.11] CARHSRLVRYFEFW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'T' (1 nucleotides at position 54 to 54 in the user submitted sequence), (cause frameshift); tctctggtggctccatcaacagtggtagttatcactgggcctggatccgccagTcccccagggaaggggctggagtggattgggagcgtctcctatggtgggaacacctactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgtgcgagacattcgagactggtacggtactttgaattctggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatcgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [289, 290] g92>a,S31>N(- - -)|a103>g,S35>G(+ + -)|c111>t|t112>c,Y38>H(+ - -)|g119>c,G40>A(- + -)|t165>c|a166>g,I56>V(+ - +)|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a175>g,S59>G(+ + -)|g191>a,S64>N(- - -)|a215>t,K72>M(- + -)|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|c236>t,S79>F(- - -)|a240>c|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c261>g,F87>L(+ - -)|c271>g,L91>V(+ - +)|a291>g| g92>a,S31>N(- - -)|a103>g,S35>G(+ + -)|c111>t|t112>c,Y38>H(+ - -)| g119>c,G40>A(- + -)|t165>c| a166>g,I56>V(+ - +)|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a175>g,S59>G(+ + -)|g191>a,S64>N(- - -)| a215>t,K72>M(- + -)|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|c236>t,S79>F(- - -)|a240>c|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c261>g,F87>L(+ - -)|c271>g,L91>V(+ - +)|a291>g| 320 229 208 21 4 17 6 2 3 3 2 1 1 0 1 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 1 3 1 1 1 1 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 0 0 1 0 0 0 0 1 0 0 0 30 21 16 5 0 5 2 1 0 1 1 0 0 0 0 0 0 0 117 114 104 10 2 8 3 0 2 0 1 1 1 0 0 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcaacagtggtagttatcac tgggcctggatccgccagcccccagggaaggggctggagtggattgggagc gtctcctatggtgggaacacc tactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgt gcgagacattcgagactggtacggtactttgaattc ARHSRLVRYFEF IGHV4-39 IGHD5-18 IGHJ4 ggtggctccatcaacagtggtagttatcac tgggcctggatccgccagcccccagggaaggggctggagtggattgggagc gtctcctatggtgggaacacc tactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgt gcgagacattcgagactggtacggtactttgaattc IGA1 21 216 14 7 5 9 9 12 3 1 9 8 JY8QFUQ01AO5H2 16023 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F (see comment) 734 81.65 178/218 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD6-6*01 F 1 8 8 11 8.8.11 [1.17.38.11] CAKVDSSSAWDYW in-frame + Low V-REGION identity (81,65% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release cctctggattcaagtttcatgaatatgccatgatctgggtccgccaggctccaggcaagggtccggaatgggtctcgtctattagtagtactagtaaatacatctattatgcacagtcagtggagggccgattcaccatctccagagacgacgccgagaacgcactgttcctccagatgagcagcctgagtgtcgacgacacggctatctattactgcgcgaaagtcgatagcagttcggcctgggactattggggcccgggaaccctggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [282] c86>a,T29>K(- - -)|c87>g,T29>K(- - -)|c90>t|a103>c,S35>H(+ - -)|g104>a,S35>H(+ - -)|a106>g,S36>E(- - -)|g107>a,S36>E(- - -)|c108>a,S36>E(- - -)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)|a119>t,N40>I(- - -)|g141>c|g147>t|t149>c,L50>P(- - -)|g153>a|a162>g|c165>t|g176>c,S59>T(+ - +)|g188>a,S63>K(- - -)|t189>a,S63>K(- - -)|a195>c|c198>t|c201>t|g205>c,D69>Q(+ - -)|c207>g,D69>Q(+ - -)|a214>g,K72>E(+ - -)|a244>g,N82>D(+ + -)|a250>g,K84>E(+ - -)|t256>g,S86>A(- + -)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|g267>c|a270>g|a275>g,N92>S(- - -)|a285>t,R95>S(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)|t312>c|g317>a,R106>K(+ + +)| c86>a,T29>K(- - -)|c87>g,T29>K(- - -)|c90>t|a103>c,S35>H(+ - -)|g104>a,S35>H(+ - -)|a106>g,S36>E(- - -)|g107>a,S36>E(- - -)|c108>a,S36>E(- - -)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)| a119>t,N40>I(- - -)|g141>c|g147>t|t149>c,L50>P(- - -)|g153>a|a162>g|c165>t| g176>c,S59>T(+ - +)|g188>a,S63>K(- - -)|t189>a,S63>K(- - -)|a195>c| c198>t|c201>t|g205>c,D69>Q(+ - -)|c207>g,D69>Q(+ - -)|a214>g,K72>E(+ - -)|a244>g,N82>D(+ + -)|a250>g,K84>E(+ - -)|t256>g,S86>A(- + -)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|g267>c|a270>g|a275>g,N92>S(- - -)|a285>t,R95>S(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)|t312>c| g317>a,R106>K(+ + +)|a320>t| 319 (320) 225 (226) 184 41 (42) 12 (13) 29 8 6 5 3 2 2 3 (4) 1 7 2 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 14 10 1 9 2 2 1 0 1 2 0 0 1 1 0 0 51 51 44 7 5 2 1 1 1 1 0 0 1 0 1 0 1 0 30 24 20 4 1 3 0 1 0 0 1 0 0 1 1 0 0 0 117 114 95 19 5 14 5 1 3 2 0 0 2 0 4 1 0 1 7 (8) 7 6 1 (2) 0 (1) 1 0 1 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaagtttcatgaatatgcc atgatctgggtccgccaggctccaggcaagggtccggaatgggtctcgtct attagtagtactagtaaatacatc tattatgcacagtcagtggagggccgattcaccatctccagagacgacgccgagaacgcactgttcctccagatgagcagcctgagtgtcgacgacacggctatctattactgc gcgaaagtcgatagcagttcggcctgggactat AKVDSSSAWDY IGHV3-21 IGHD6-6 IGHJ4 ggattcaagtttcatgaatatgcc atgatctgggtccgccaggctccaggcaagggtccggaatgggtctcgtct attagtagtactagtaaatacatc tattatgcacagtcagtggagggccgattcaccatctccagagacgacgccgagaacgcactgttcctccagatgagcagcctgagtgtcgacgacacggctatctattactgc gcgaaagtcgatagcagttcggcctgggactat IGA1 40 213 21 19 10 22 11 18 10 2 16 12 JY8QFUQ01AO7UP 14655 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 838 87.21 191/219 nt 86.76 190/219 nt Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [2.17.38.11] CTRDIGGRYSYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 38 of V-REGION: 'G' (1 nucleotides at position 29 to 29 in the user submitted sequence), (cause frameshift); ctccgggagttcacctttagtagtttctGggatgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacggactaatgagtatgggagtatcacaaactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgtacaagggacatcggtgggcgctactcctactggggccagggagtcctggtctccgtctcatcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [281] g73>c,A25>L(+ - +)|c74>t,A25>L(+ - +)|t76>c,S26>R(- - -)|c77>g,S26>R(- - -)|t78>g,S26>R(- - -)|g80>a,G27>E(- - -)|a81>g,G27>E(- - -)|c90>t|c108>t|a110>t,Y37>F(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g247>a,A83>T(- - -)|c257>g,T86>R(- - -)|g261>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|g313>a,A105>T(- - -)|a318>g| g73>c,A25>L(+ - +)|c74>t,A25>L(+ - +)|t76>c,S26>R(- - -)|c77>g,S26>R(- - -)|t78>g,S26>R(- - -)| g80>a,G27>E(- - -)|a81>g,G27>E(- - -)|c90>t|c108>t|a110>t,Y37>F(- + -)| c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g247>a,A83>T(- - -)|c257>g,T86>R(- - -)|g261>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| g313>a,A105>T(- - -)|a318>g| 320 227 197 30 6 24 5 8 4 2 0 0 1 0 1 2 3 4 78 6 1 5 0 5 0 0 1 1 0 0 0 0 1 1 0 1 36 24 19 5 2 3 1 1 2 0 0 0 1 0 0 0 0 0 51 51 46 5 2 3 1 1 1 0 0 0 0 0 0 0 0 2 30 24 18 6 0 6 1 1 0 1 0 0 0 0 0 0 2 1 117 114 107 7 1 6 1 4 0 0 0 0 0 0 0 1 1 0 8 8 6 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctccgg gagttcacctttagtagtttctgg atgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgt acaagggacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 gagttcacctttagtagtttctgg atgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgt acaagggacatcggtgggcgctactcctac IGA2 23 213 15 8 10 14 5 9 3 2 9 9 JY8QFUQ01AO8B3 14795 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 927 92.17 200/217 nt 90.78 197/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [2.16.38.11] CVRAGFGELSGFDCW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'C' (1 nucleotides at position 15 to 15 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 40 of V-REGION: 1 nucleotides (from position 35 in the user submitted sequence), (cause frameshift)in FR2-IMGT, from codon 45 of V-REGION: 1 nucleotides (from position 48 in the user submitted sequence), (cause frameshift) gcctctggattcacCctttggttattatggcatgactgggtccgccaactccgggggagggggctgagtgggtctctggtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [289] a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a138>g|a142>g,K48>E(+ - -)|c148>g,L50>A(+ - +)|t149>c,L50>A(+ - +)|g150>t,L50>A(+ - +)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a138>g|a142>g,K48>E(+ - -)|c148>g,L50>A(+ - +)|t149>c,L50>A(+ - +)|g150>t,L50>A(+ - +)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 224 (225) 206 18 (19) 3 (4) 15 3 3 1 2 1 (2) 0 2 0 2 2 2 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 49 44 5 1 4 2 0 0 1 0 0 0 0 0 1 1 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| gcctct ggattcacctttggttattatggc atgactgggtccgccaactccgggggagggggctgagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgactgggtccgccaactccgggggagggggctgagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 17 211 8 9 3 9 5 8 3 0 10 4 JY8QFUQ01AO8X8 8426 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 720 81.57 177/217 nt NA Homsap IGHJ4*02 F (see comment) 177 85.42 41/48 nt Homsap IGHD4-23*01 ORF 2 8 8 17 8.8.17 [1.17.38.11] CAKVKGYGGNWFQPHFDRW in-frame + Low V-REGION identity (81,57% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ1*01 (highest number of consecutive identical nucleotides) ctctggattctccttcgaggggtctgtcgtgacctgggtccgacaggctccagggaaggggctggagtgggtctcaggtatctacggtggtgatggtacatcattttacgcagacttcgcgaagggccgattcaccgtctccagagacaattccaaggacacggtctatctgcagatgaacggcctgagagtcgaagactcggcccgttattattgtgcgaaagtgaagggctacggcggcaattggttccagcctcactttgaccgctggggccagggcaccctggtcaccgtctccgcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [299] a85>t,T29>S(+ - +)|t90>c|a103>g,S35>E(- - -)|g104>a,S35>E(- - -)|c105>g,S35>E(- - -)|a106>g,S36>G(+ + -)|c108>g,S36>G(+ + -)|a110>c,Y37>S(+ - -)|c113>t,A38>V(+ - +)|a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|c129>a|c164>g,A55>G(- + -)|t168>c|a169>t,S57>Y(+ - -)|g170>a,S57>Y(+ - -)|t171>c,S57>Y(+ - -)|a175>g,S59>G(+ + -)|g185>a,G62>D(- - -)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)|a193>t,T65>S(+ - +)|a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|c209>t,S70>F(- - -)|t212>c,V71>A(+ - +)|g225>a|a232>g,I78>V(+ - +)|a253>g,N85>D(+ + -)|c259>g,L87>V(+ - +)|g261>c,L87>V(+ - +)|a270>g|a277>g,S93>G(+ + -)|c287>t,A96>V(+ - +)|g291>a|a295>t,T99>S(+ - +)|g301>c,V101>R(- - -)|t302>g,V101>R(- - -)|a303>t,V101>R(- - -)|c309>t| a85>t,T29>S(+ - +)|t90>c|a103>g,S35>E(- - -)|g104>a,S35>E(- - -)|c105>g,S35>E(- - -)|a106>g,S36>G(+ + -)|c108>g,S36>G(+ + -)|a110>c,Y37>S(+ - -)|c113>t,A38>V(+ - +)| a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|c129>a|c164>g,A55>G(- + -)| t168>c|a169>t,S57>Y(+ - -)|g170>a,S57>Y(+ - -)|t171>c,S57>Y(+ - -)|a175>g,S59>G(+ + -)|g185>a,G62>D(- - -)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)|a193>t,T65>S(+ - +)| a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|c209>t,S70>F(- - -)|t212>c,V71>A(+ - +)|g225>a|a232>g,I78>V(+ - +)|a253>g,N85>D(+ + -)|c259>g,L87>V(+ - +)|g261>c,L87>V(+ - +)|a270>g|a277>g,S93>G(+ + -)|c287>t,A96>V(+ - +)|g291>a|a295>t,T99>S(+ - +)|g301>c,V101>R(- - -)|t302>g,V101>R(- - -)|a303>t,V101>R(- - -)|c309>t| a320>t| 319 (320) 224 (225) 184 40 (41) 7 (8) 33 8 5 5 4 1 2 6 (7) 0 4 4 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 15 9 1 8 2 1 1 1 1 0 1 0 0 2 0 0 51 51 47 4 1 3 1 0 0 0 0 1 0 0 1 1 0 0 30 24 15 9 1 8 1 2 0 2 0 1 2 0 1 0 0 0 117 114 96 18 4 14 4 2 4 1 0 0 3 0 2 1 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattctccttcgaggggtctgtc gtgacctgggtccgacaggctccagggaaggggctggagtgggtctcaggt atctacggtggtgatggtacatca ttttacgcagacttcgcgaagggccgattcaccgtctccagagacaattccaaggacacggtctatctgcagatgaacggcctgagagtcgaagactcggcccgttattattgt gcgaaagtgaagggctacggcggcaattggttccagcctcactttgaccgc AKVKGYGGNWFQPHFDR IGHV3-23 IGHD4-23 IGHJ4 ggattctccttcgaggggtctgtc gtgacctgggtccgacaggctccagggaaggggctggagtgggtctcaggt atctacggtggtgatggtacatca ttttacgcagacttcgcgaagggccgattcaccgtctccagagacaattccaaggacacggtctatctgcagatgaacggcctgagagtcgaagactcggcccgttattattgt gcgaaagtgaagggctacggcggcaattggttccagcctcactttgaccgc IGA2 40 213 22 18 10 20 12 20 5 2 17 16 JY8QFUQ01AOCIF 15486 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 968 94.04 205/218 nt NA Homsap IGHJ6*02 F 212 87.27 48/55 nt NA 8 8 10 8.8.10 [1.17.38.11] CARDRYYVVDVW in-frame - cctctggattcaccttcagtgccacctggatgcactgggtccgccaagctccagggcaggggctggtgtgggtctctcatattaatggtgatgggagtagcacaagttacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacggtgtatttgcaaatgaacagtctgagagccgaggacacggctgtatactactgtgcaagagatcgatactacgttgtggacgtctggggccaagggaccacggtcaccgtctcctccgcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [280] a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|t109>a,Y37>T(+ - -)|a110>c,Y37>T(+ - -)|a142>c,K48>Q(+ - -)|a162>t|g164>a,R55>H(- - +)|a172>g,S58>G(+ + -)|c198>t|c259>g,L87>V(+ - +)|c265>t|g303>a|t306>c| a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|t109>a,Y37>T(+ - -)|a110>c,Y37>T(+ - -)| a142>c,K48>Q(+ - -)|a162>t|g164>a,R55>H(- - +)| a172>g,S58>G(+ + -)| c198>t|c259>g,L87>V(+ - +)|c265>t|g303>a|t306>c| 320 226 213 13 5 8 2 2 2 1 2 0 1 1 1 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 0 0 0 1 0 0 1 1 0 0 0 51 51 48 3 1 2 0 1 0 0 1 0 1 0 0 0 0 0 30 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 117 114 109 5 4 1 0 1 2 1 0 0 0 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtgccacctgg atgcactgggtccgccaagctccagggcaggggctggtgtgggtctctcat attaatggtgatgggagtagcaca agttacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacggtgtatttgcaaatgaacagtctgagagccgaggacacggctgtatactactgt gcaagagatcgatactacgttgtggacgtc ARDRYYVVDV IGHV3-74 IGHJ6 ggattcaccttcagtgccacctgg atgcactgggtccgccaagctccagggcaggggctggtgtgggtctctcat attaatggtgatgggagtagcaca agttacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacggtgtatttgcaaatgaacagtctgagagccgaggacacggctgtatactactgt gcaagagatcgatactacgttgtggacgtc IGG2 13 213 7 6 4 6 3 7 5 0 3 5 JY8QFUQ01AODPS 9814 productive Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F or Homsap IGHV4-34*07 F (see comment) 733 82.41 178/216 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-15*01 F 2 8 7 15 8.7.15 [2.17.38.11] CARDWGGSFDRYYFDYW in-frame - Low V-REGION identity (82,41% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release gtccaccgtgggtctttcactggttatcgctggagctggatccgccaggccccagggaagggactggagtgggtgggggacatcaactttaaaggaagcaccaattacaacccgtccctgaagagtcgactcaccatattagcagacttgtccaggaatcggttctctctagacctaagtggtgtgaccgccgcggacacggctatgtattattgtgcgagagattggggtggaagcttcgataggtattattttgactactggggccagggaatcctggtcactgtctcctcagcatccccgaccagccccaagatcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [292] t76>c,Y26>H(+ - -)|t78>c,Y26>H(+ - -)|g79>c,G27>R(- - -)|c87>t|g104>c,S35>T(+ - +)|c111>t|t112>c,Y38>R(- - -)|a113>g,Y38>R(- - -)|c133>g,P45>A(- - -)|g147>a|a157>g,I53>V(+ - +)|t159>g,I53>V(+ - +)|a165>c,E55>D(+ - +)|t171>c|c172>t,H58>F(- - -)|a173>t,H58>F(- - -)|g176>a,S59>K(- - -)|t177>a,S59>K(- - -)|c198>t|c213>g|g226>c,V76>L(+ - +)|c236>t,S79>L(- - -)|t239>c,V80>A(+ - +)|a244>t,T82>L(- - -)|c245>t,T82>L(- - -)|a251>g,K84>R(+ + +)|c255>t|a257>g,Q86>R(+ - -)|c264>t|g267>a|a268>g,K90>D(+ - -)|g270>c,K90>D(+ - -)|g273>a|c276>t|t277>g,S93>G(+ + -)|c278>g,S93>G(+ + -)|g301>a,V101>M(+ - -)|c309>t| t76>c,Y26>H(+ - -)|t78>c,Y26>H(+ - -)| g79>c,G27>R(- - -)|c87>t|g104>c,S35>T(+ - +)|c111>t|t112>c,Y38>R(- - -)|a113>g,Y38>R(- - -)| c133>g,P45>A(- - -)|g147>a|a157>g,I53>V(+ - +)|t159>g,I53>V(+ - +)|a165>c,E55>D(+ - +)| t171>c|c172>t,H58>F(- - -)|a173>t,H58>F(- - -)|g176>a,S59>K(- - -)|t177>a,S59>K(- - -)| c198>t|c213>g|g226>c,V76>L(+ - +)|c236>t,S79>L(- - -)|t239>c,V80>A(+ - +)|a244>t,T82>L(- - -)|c245>t,T82>L(- - -)|a251>g,K84>R(+ + +)|c255>t|a257>g,Q86>R(+ - -)|c264>t|g267>a|a268>g,K90>D(+ - -)|g270>c,K90>D(+ - -)|g273>a|c276>t|t277>g,S93>G(+ + -)|c278>g,S93>G(+ + -)|g301>a,V101>M(+ - -)|c309>t| g320>a| 319 (320) 223 (224) 185 38 (39) 12 (13) 26 5 5 (6) 10 5 1 0 2 1 4 3 0 2 78 6 4 2 0 2 0 0 0 2 0 0 0 0 0 0 0 0 36 24 18 6 2 4 1 0 2 1 0 0 0 0 2 0 0 0 51 51 46 5 1 4 1 1 0 0 1 0 0 0 0 1 0 1 30 21 16 5 1 4 0 1 1 1 0 0 1 1 0 0 0 0 117 114 94 20 8 12 3 3 7 1 0 0 1 0 2 2 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtccac cgtgggtctttcactggttatcgc tggagctggatccgccaggccccagggaagggactggagtgggtgggggac atcaactttaaaggaagcacc aattacaacccgtccctgaagagtcgactcaccatattagcagacttgtccaggaatcggttctctctagacctaagtggtgtgaccgccgcggacacggctatgtattattgt gcgagagattggggtggaagcttcgataggtattattttgactac ARDWGGSFDRYYFDY IGHV4-34 IGHD2-15 IGHJ4 cgtgggtctttcactggttatcgc tggagctggatccgccaggccccagggaagggactggagtgggtgggggac atcaactttaaaggaagcacc aattacaacccgtccctgaagagtcgactcaccatattagcagacttgtccaggaatcggttctctctagacctaagtggtgtgaccgccgcggacacggctatgtattattgt gcgagagattggggtggaagcttcgataggtattattttgactac IGA1 36 210 23 13 15 22 8 14 9 3 16 8 JY8QFUQ01AODUI 16225 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 937 92.24 202/219 nt NA Homsap IGHJ6*02 F (see comment) 205 83.05 49/59 nt Homsap IGHD5-24*01 ORF 3 8 8 11 8.8.11 [2.17.38.11] CARPRYGYGLDVW in-frame - Other possibilities: Homsap_IGHJ4*01 (shorter alignment but highest percentage of identity) ggttctggatacgactttaacaactactggatcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggaatcatctttcctggtgactctgatatcagatatagtccgtcgttccaaggccaggtcaccatctcagtcgacaagtccatcggcaccgcctacctgcaatggagcggcctgaaggcctcggacaccgccacttattattgtgcgagacctagatacggatacggcttggacgtctggggccaagggaccacggtcaccgtctcctcagccccccaccaagggcccatcggtcttcccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 93 94 [286, 285] a85>g,S29>D(- - -)|g86>a,S29>D(- - -)|c104>a,T35>N(- + -)|g107>a,S36>N(- - -)|g162>a|a170>t,Y57>F(- + -)|c194>t,T65>I(- - -)|c201>t|c204>t|c210>g|c239>t,A80>V(+ - +)|a253>g,S85>G(+ + -)|g270>a|a277>g,S93>G(+ + -)|t302>c,M101>T(- - -)|g303>t,M101>T(- - -)|c309>t| a85>g,S29>D(- - -)|g86>a,S29>D(- - -)|c104>a,T35>N(- + -)|g107>a,S36>N(- - -)| g162>a| a170>t,Y57>F(- + -)|c194>t,T65>I(- - -)| c201>t|c204>t|c210>g|c239>t,A80>V(+ - +)|a253>g,S85>G(+ + -)|g270>a|a277>g,S93>G(+ + -)|t302>c,M101>T(- - -)|g303>t,M101>T(- - -)|c309>t| a320>c| 319 (320) 226 (227) 209 17 (18) 6 (7) 11 3 4 5 1 0 (1) 1 1 0 0 1 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 2 0 0 0 1 0 0 0 0 0 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 1 0 0 0 1 0 0 0 0 0 117 114 104 10 5 5 2 1 4 1 0 0 0 0 0 1 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ggttct ggatacgactttaacaactactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggaatc atctttcctggtgactctgatatc agatatagtccgtcgttccaaggccaggtcaccatctcagtcgacaagtccatcggcaccgcctacctgcaatggagcggcctgaaggcctcggacaccgccacttattattgt gcgagacctagatacggatacggcttggacgtc ARPRYGYGLDV IGHV5-51 IGHD5-24 IGHJ6 ggatacgactttaacaactactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggaatc atctttcctggtgactctgatatc agatatagtccgtcgttccaaggccaggtcaccatctcagtcgacaagtccatcggcaccgcctacctgcaatggagcggcctgaaggcctcggacaccgccacttattattgt gcgagacctagatacggatacggcttggacgtc IGG1 17 213 13 4 9 12 4 5 6 0 5 6 JY8QFUQ01AOFCE 15007 productive Homsap IGHV3-30*01 F 992 94.57 209/221 nt NA Homsap IGHJ4*02 F 240 100 48/48 nt Homsap IGHD2-15*01 F 3 8 8 16 8.8.16 [2.17.38.11] CAKDELPWDTIEYYFDYW in-frame - aggcctctggattcaccttcaatacctattctatgcactgggtccgccaggctccaggcaaggggctagagtgggtgtcagttatttcatatgatggaagtaagaaatactacgcggactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatctgcaaatgaacagcctgagacctgaggacacggctgtttattactgtgcgaaagatgagctgccttgggataccatagagtactactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [300] c71>a|a72>g|g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)|g112>t,A38>S(- + -)|g160>t,A54>S(- + -)|a168>t|t192>g,N64>K(+ - -)|a204>g|c259>g,L87>V(+ - +)|g286>c,A96>P(- - -)|g303>t|g317>a,R106>K(+ + +)| c71>a|a72>g| g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)|g112>t,A38>S(- + -)| g160>t,A54>S(- + -)| a168>t|t192>g,N64>K(+ - -)| a204>g|c259>g,L87>V(+ - +)|g286>c,A96>P(- - -)|g303>t| g317>a,R106>K(+ + +)| 320 229 216 13 5 8 2 2 0 0 0 1 1 0 2 1 3 1 78 8 6 2 2 0 1 0 0 0 0 1 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 0 1 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 30 24 22 2 1 1 0 0 0 0 0 0 1 0 0 0 0 1 117 114 110 4 2 2 1 0 0 0 0 0 0 0 1 1 1 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| aggcctct ggattcaccttcaatacctattct atgcactgggtccgccaggctccaggcaaggggctagagtgggtgtcagtt atttcatatgatggaagtaagaaa tactacgcggactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatctgcaaatgaacagcctgagacctgaggacacggctgtttattactgt gcgaaagatgagctgccttgggataccatagagtactactttgactac AKDELPWDTIEYYFDY IGHV3-30 IGHD2-15 IGHJ4 ggattcaccttcaatacctattct atgcactgggtccgccaggctccaggcaaggggctagagtgggtgtcagtt atttcatatgatggaagtaagaaa tactacgcggactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatctgcaaatgaacagcctgagacctgaggacacggctgtttattactgt gcgaaagatgagctgccttgggataccatagagtactactttgactac IGA1 10 213 2 8 1 7 1 3 2 1 3 4 JY8QFUQ01AOFPE 14976 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 842 87.61 191/218 nt NA Homsap IGHJ6*02 F (see comment) 193 79.03 49/62 nt Homsap IGHD6-6*01 F 1 8 8 17 8.8.17 [1.17.38.11] CVRDSGPSGYARYESMDVW in-frame - Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) cctctggattcagcctcacttcctatggcatgaactgggtccgccaggctccagggagggggctggagtgggtctcacacgttaatatgggtagtactcacatatactacgtaggctccgtgaggggccgattcaccatctccagagacgacgccaagaactcagtgtatctgcagatgaacaacttgagagccgaggacacggctctatattactgtgtgagagatagtggcccgtccggctacgctaggtacgagtctatggacgtctggggccaagggaccgcggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [301] c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|g104>c,S35>T(+ - +)|a106>t|g107>c|a112>g,S38>G(+ + -)|a143>g,K48>R(+ + +)|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)|a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|g173>t,S58>M(- - -)|t174>g,S58>M(- - -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)|c203>t,A68>V(+ - +)|a206>g,D69>G(- - -)|a210>c|a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|c259>g,L87>V(+ - +)|a270>g|g278>a,S93>N(- - -)|c280>t|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)|c314>t,A105>V(+ - +)| c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|g104>c,S35>T(+ - +)|a106>t|g107>c|a112>g,S38>G(+ + -)| a143>g,K48>R(+ + +)|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)| a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|g173>t,S58>M(- - -)|t174>g,S58>M(- - -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)| c203>t,A68>V(+ - +)|a206>g,D69>G(- - -)|a210>c|a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|c259>g,L87>V(+ - +)|a270>g|g278>a,S93>N(- - -)|c280>t|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| c314>t,A105>V(+ - +)| 320 226 198 28 5 23 8 3 3 3 1 1 1 0 4 2 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 2 4 1 0 0 1 0 0 1 0 2 1 0 0 51 51 48 3 0 3 1 0 0 1 0 1 0 0 0 0 0 0 30 24 17 7 0 7 2 1 0 1 0 0 0 0 1 0 1 1 117 114 103 11 3 8 4 2 2 0 1 0 0 0 1 1 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcagcctcacttcctatggc atgaactgggtccgccaggctccagggagggggctggagtgggtctcacac gttaatatgggtagtactcacata tactacgtaggctccgtgaggggccgattcaccatctccagagacgacgccaagaactcagtgtatctgcagatgaacaacttgagagccgaggacacggctctatattactgt gtgagagatagtggcccgtccggctacgctaggtacgagtctatggacgtc VRDSGPSGYARYESMDV IGHV3-21 IGHD6-6 IGHJ6 ggattcagcctcacttcctatggc atgaactgggtccgccaggctccagggagggggctggagtgggtctcacac gttaatatgggtagtactcacata tactacgtaggctccgtgaggggccgattcaccatctccagagacgacgccaagaactcagtgtatctgcagatgaacaacttgagagccgaggacacggctctatattactgt gtgagagatagtggcccgtccggctacgctaggtacgagtctatggacgtc IGG2 27 213 16 11 5 13 11 14 3 2 11 11 JY8QFUQ01AOGGT 14213 productive Homsap IGHV3-7*01 F 932 92.2 201/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-24*01 ORF 2 8 8 12 8.8.12 [1.17.38.11] CARWRGQQSEMEYW in-frame - cctctggattcacctttagtaactattacatgagttgggtccgccaggctccagggaaggggctggagtacgtggccagcataaaacaagatgaaggtcagacatactatgcgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatcttcaaatgaacagcctgagagtcgaggacacggctgtgtatcactgtgcgcggtggagagggcaacaatccgaaatggaatactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [285] g107>a,S36>N(- - -)|g113>a,W38>Y(- + -)|g114>c,W38>Y(- + -)|c120>t|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|a164>g,N55>S(- - -)|g171>a|g185>a,G62>E(- - -)|a187>g,S63>G(+ + -)|g190>c,E64>Q(+ + -)|a194>c,K65>T(- - -)|t203>c,V68>A(+ - +)|a206>g,D69>G(- - -)|g267>t|c287>t,A96>V(+ - +)|t307>c,Y103>H(+ - -)| g107>a,S36>N(- - -)|g113>a,W38>Y(- + -)|g114>c,W38>Y(- + -)| c120>t|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|a164>g,N55>S(- - -)| g171>a|g185>a,G62>E(- - -)|a187>g,S63>G(+ + -)|g190>c,E64>Q(+ + -)|a194>c,K65>T(- - -)| t203>c,V68>A(+ - +)|a206>g,D69>G(- - -)|g267>t|c287>t,A96>V(+ - +)|t307>c,Y103>H(+ - -)| a316>c|a318>g|g319>t|a320>g| 315 (320) 221 (226) 204 (205) 17 (21) 3 (7) 14 3 (5) 5 2 2 1 (2) 0 0 0 3 0 1 (2) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 2 0 0 0 0 0 0 1 0 0 0 51 51 47 4 1 3 1 1 1 0 0 0 0 0 1 0 0 0 30 24 19 5 1 4 1 2 0 0 1 0 0 0 1 0 0 0 117 114 109 5 1 4 1 0 1 2 0 0 0 0 0 0 1 0 3 (8) 3 3 (4) 0 (4) 0 (4) 0 0 (2) 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagtaactattac atgagttgggtccgccaggctccagggaaggggctggagtacgtggccagc ataaaacaagatgaaggtcagaca tactatgcgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatcttcaaatgaacagcctgagagtcgaggacacggctgtgtatcactgt gcgcggtggagagggcaacaatccgaaatggaatac ARWRGQQSEMEY IGHV3-7 IGHD5-24 IGHJ4 ggattcacctttagtaactattac atgagttgggtccgccaggctccagggaaggggctggagtacgtggccagc ataaaacaagatgaaggtcagaca tactatgcgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatcttcaaatgaacagcctgagagtcgaggacacggctgtgtatcactgt gcgcggtggagagggcaacaatccgaaatggaatac IGA1 17 213 12 5 7 11 5 6 2 1 7 7 JY8QFUQ01AOGS5 13941 productive (see comment) Homsap IGHV1-8*01 F 907 92.02 196/213 nt 91.55 195/213 nt Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD1-26*01 F 1 8 8 11 8.8.11 [0.16.38.11] CAREGLLGSLGSW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 49 of V-REGION: 1 nucleotides (from position 58 in the user submitted sequence), (cause frameshift) aggatacaccttcatcagttatgatatcaattgggtgcgacaggccactggacaaggcttgagtggatgggatggatgaaccctaacagcggtaacacagggtttgcacagaggttccagggcagagtaaccatgaccaggaacatctccataaacacggcctacatggagctgaccaacctgacatctgatgacacggccgtatattattgtgcgagagaaggtctattgggcagcctaggatcttggggccagggaaccctggtcaccgtcgcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [277] t78>a|c104>t,T35>I(- - -)|c120>t|t186>c|c198>g|a200>t,Y67>F(- + -)|a209>g,K70>R(+ + +)|c228>a|c245>t,T82>I(- - -)|g254>a,S85>N(- - -)|a258>g|g275>c,S92>T(+ - +)|g278>a,S93>N(- - -)|g284>c,R95>T(- - -)|g291>t,E97>D(+ - +)|g303>a|c309>t| t78>a| c104>t,T35>I(- - -)| c120>t| t186>c| c198>g|a200>t,Y67>F(- + -)|a209>g,K70>R(+ + +)|c228>a|c245>t,T82>I(- - -)|g254>a,S85>N(- - -)|a258>g|g275>c,S92>T(+ - +)|g278>a,S93>N(- - -)|g284>c,R95>T(- - -)|g291>t,E97>D(+ - +)|g303>a|c309>t| g320>a| 319 (320) 220 (221) 203 17 (18) 8 (9) 9 2 3 (4) 4 1 0 1 1 1 2 1 1 0 78 1 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 50 49 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 101 13 5 8 2 3 2 0 0 1 1 0 2 1 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,118-119(FR2)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|aa,241-242(FR3)|ta,251-252(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,251-252(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|agtt,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,118-121(FR2)|aacc,169-172(CDR2)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| a ggatacaccttcatcagttatgat atcaattgggtgcgacaggccactggacaaggcttgagtggatgggatgg atgaaccctaacagcggtaacaca gggtttgcacagaggttccagggcagagtaaccatgaccaggaacatctccataaacacggcctacatggagctgaccaacctgacatctgatgacacggccgtatattattgt gcgagagaaggtctattgggcagcctaggatct AREGLLGSLGS IGHV1-8 IGHD1-26 IGHJ4 ggatacaccttcatcagttatgat atcaattgggtgcgacaggccactggacaaggcttgagtggatgggatgg atgaaccctaacagcggtaacaca gggtttgcacagaggttccagggcagagtaaccatgaccaggaacatctccataaacacggcctacatggagctgaccaacctgacatctgatgacacggccgtatattattgt gcgagagaaggtctattgggcagcctaggatct IGA1 16 212 10 6 7 12 3 4 6 1 8 1 JY8QFUQ01AOJXD 17079 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 791 85.58 184/215 nt 85.12 183/215 nt Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F 141 77.08 37/48 nt Homsap IGHD3-10*01 F 1 8 7 13 8.7.13 [1.17.38.11] CATVRHLGERIFDFW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'C' (1 nucleotides at position 12 to 12 in the user submitted sequence), (cause frameshift); tctctgggggcCaccatgagtagtttctactggagctgggttcggcagtccccagggaggggactggagtggattggatttgtttcttacagtgggcccaccaactacagcccctccctcaagagtcgagtcaacttatcactggacgcggccaacaaacagttctctttgcagctgcgttctgtgaccgctgcggacacggccatttattactgtgcgacagtacgacatctcggggaacgaatattcgatttctggggccagggtatcctggtcaccgtcccctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [286] t81>g|t85>a,S29>T(+ - +)|c90>g,I30>M(+ + -)|a110>t,Y37>F(- + -)|a124>g,I42>V(+ - +)|c126>t,I42>V(+ - +)|c133>t,P45>S(+ - -)|a143>g,K48>R(+ + +)|g162>a|a164>t,Y55>F(- + -)|a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|a170>c,Y57>S(+ - -)|a190>c,S64>P(+ - -)|g191>c,S64>P(+ - -)|a203>g,N68>S(- - -)|c230>a,T77>N(- + -)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|a244>g,T82>A(- - -)|t247>g,S83>A(- + -)|g252>c,K84>N(+ - -)|c255>a,N85>K(+ - -)|c264>t|c265>t|a268>c,K90>Q(+ - -)|a274>c,S92>R(- - -)|c276>t,S92>R(- - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|g317>c,R106>T(- - -)| t81>g|t85>a,S29>T(+ - +)|c90>g,I30>M(+ + -)|a110>t,Y37>F(- + -)| a124>g,I42>V(+ - +)|c126>t,I42>V(+ - +)|c133>t,P45>S(+ - -)|a143>g,K48>R(+ + +)|g162>a|a164>t,Y55>F(- + -)| a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|a170>c,Y57>S(+ - -)|a190>c,S64>P(+ - -)|g191>c,S64>P(+ - -)| a203>g,N68>S(- - -)|c230>a,T77>N(- + -)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|a244>g,T82>A(- - -)|t247>g,S83>A(- + -)|g252>c,K84>N(+ - -)|c255>a,N85>K(+ - -)|c264>t|c265>t|a268>c,K90>Q(+ - -)|a274>c,S92>R(- - -)|c276>t,S92>R(- - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| g317>c,R106>T(- - -)|a320>t| 319 (320) 222 (223) 190 32 (33) 4 (5) 28 6 2 6 0 4 2 3 (4) 1 4 1 1 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 0 0 0 0 1 1 0 1 0 1 51 51 45 6 1 5 2 1 2 0 0 0 1 0 0 0 0 0 30 21 16 5 0 5 1 0 1 0 2 0 0 0 1 0 0 0 117 114 98 16 2 14 3 1 3 0 2 2 1 0 2 0 1 1 7 (8) 7 6 1 (2) 0 (1) 1 0 0 0 0 0 0 0 (1) 0 1 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct gggggcaccatgagtagtttctac tggagctgggttcggcagtccccagggaggggactggagtggattggattt gtttcttacagtgggcccacc aactacagcccctccctcaagagtcgagtcaacttatcactggacgcggccaacaaacagttctctttgcagctgcgttctgtgaccgctgcggacacggccatttattactgt gcgacagtacgacatctcggggaacgaatattcgatttc ATVRHLGERIFDF IGHV4-59 IGHD3-10 IGHJ4 gggggcaccatgagtagtttctac tggagctgggttcggcagtccccagggaggggactggagtggattggattt gtttcttacagtgggcccacc aactacagcccctccctcaagagtcgagtcaacttatcactggacgcggccaacaaacagttctctttgcagctgcgttctgtgaccgctgcggacacggccatttattactgt gcgacagtacgacatctcggggaacgaatattcgatttc IGA1 31 210 14 17 8 15 6 16 3 1 19 8 JY8QFUQ01AOLHE 1297 productive (see comment) Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F or Homsap IGHV4-34*07 F (see comment) 732 82.71 177/214 nt 82.24 176/214 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-15*01 F 2 8 7 15 8.7.15 [1.16.38.11] CARDWGGSFDRYYFDYW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (82,71% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR2-IMGT, from codon 53 of V-REGION: 1 nucleotides (from position 72 in the user submitted sequence), (cause frameshift) tccaccgtgggtctttcactggttatcgctggagctggatccgccaggccccagggaagggactggagtgggtggggacatcaactttaaaggaagcaccaattacaacccgtccctgaagagtcgactcaccatattagcagacttgtccaggaatcggttctctctagacctaagtggtgtgaccgccgcggacacggctatgtattattgtgcgagagattggggtggaagcttcgataggtattattttgactactggggccagggaatcctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [290] t76>c,Y26>H(+ - -)|t78>c,Y26>H(+ - -)|g79>c,G27>R(- - -)|c87>t|g104>c,S35>T(+ - +)|c111>t|t112>c,Y38>R(- - -)|a113>g,Y38>R(- - -)|c133>g,P45>A(- - -)|g147>a|t158>g|a165>c,E55>D(+ - +)|t171>c|c172>t,H58>F(- - -)|a173>t,H58>F(- - -)|g176>a,S59>K(- - -)|t177>a,S59>K(- - -)|c198>t|c213>g|g226>c,V76>L(+ - +)|c236>t,S79>L(- - -)|t239>c,V80>A(+ - +)|a244>t,T82>L(- - -)|c245>t,T82>L(- - -)|a251>g,K84>R(+ + +)|c255>t|a257>g,Q86>R(+ - -)|c264>t|g267>a|a268>g,K90>D(+ - -)|g270>c,K90>D(+ - -)|g273>a|c276>t|t277>g,S93>G(+ + -)|c278>g,S93>G(+ + -)|g301>a,V101>M(+ - -)|c309>t| t76>c,Y26>H(+ - -)|t78>c,Y26>H(+ - -)| g79>c,G27>R(- - -)|c87>t|g104>c,S35>T(+ - +)|c111>t|t112>c,Y38>R(- - -)|a113>g,Y38>R(- - -)| c133>g,P45>A(- - -)|g147>a|t158>g|a165>c,E55>D(+ - +)| t171>c|c172>t,H58>F(- - -)|a173>t,H58>F(- - -)|g176>a,S59>K(- - -)|t177>a,S59>K(- - -)| c198>t|c213>g|g226>c,V76>L(+ - +)|c236>t,S79>L(- - -)|t239>c,V80>A(+ - +)|a244>t,T82>L(- - -)|c245>t,T82>L(- - -)|a251>g,K84>R(+ + +)|c255>t|a257>g,Q86>R(+ - -)|c264>t|g267>a|a268>g,K90>D(+ - -)|g270>c,K90>D(+ - -)|g273>a|c276>t|t277>g,S93>G(+ + -)|c278>g,S93>G(+ + -)|g301>a,V101>M(+ - -)|c309>t| g320>a| 319 (320) 221 (222) 184 37 (38) 13 (14) 24 4 5 (6) 10 5 1 0 2 1 4 3 0 2 78 5 3 2 0 2 0 0 0 2 0 0 0 0 0 0 0 0 36 24 18 6 2 4 1 0 2 1 0 0 0 0 2 0 0 0 51 50 46 4 2 2 0 1 0 0 1 0 0 0 0 1 0 1 30 21 16 5 1 4 0 1 1 1 0 0 1 1 0 0 0 0 117 114 94 20 8 12 3 3 7 1 0 0 1 0 2 2 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tccac cgtgggtctttcactggttatcgc tggagctggatccgccaggccccagggaagggactggagtgggtggggac atcaactttaaaggaagcacc aattacaacccgtccctgaagagtcgactcaccatattagcagacttgtccaggaatcggttctctctagacctaagtggtgtgaccgccgcggacacggctatgtattattgt gcgagagattggggtggaagcttcgataggtattattttgactac ARDWGGSFDRYYFDY IGHV4-34 IGHD2-15 IGHJ4 cgtgggtctttcactggttatcgc tggagctggatccgccaggccccagggaagggactggagtgggtggggac atcaactttaaaggaagcacc aattacaacccgtccctgaagagtcgactcaccatattagcagacttgtccaggaatcggttctctctagacctaagtggtgtgaccgccgcggacacggctatgtattattgt gcgagagattggggtggaagcttcgataggtattattttgactac IGA1 35 209 22 13 15 22 7 13 10 3 14 8 JY8QFUQ01AOMW2 14675 productive Homsap IGHV3-7*03 F 860 88.53 193/218 nt NA Homsap IGHJ5*02 F 237 96.08 49/51 nt Homsap IGHD4-11*01 ORF 2 8 8 19 8.8.19 [1.17.38.11] CARDRYSSDSSYYMPNWLDPW in-frame + cctctggattcacgtttggttcgtattggatgagttgggtccgccaggctccagggaaggggctggagtgggtggccaacattcacgaagaaggaagtgaaaagaattatgtggactctgtgaagggccggttcgtcatctccagagacaacgccaagaattccctctatctgcaaatgaacagcctgagagtcgaggacacggccatatattattgtgcgagagatcgatatagtagcgattccagttactacatgcccaactggctcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [306] c87>g|a103>g,S35>G(+ + -)|a106>t|g107>c|c108>g|c120>t|a168>t|a169>c,K57>H(- - +)|g171>c,K57>H(- - +)|c172>g,Q58>E(+ + -)|t177>a,D59>E(+ - +)|g192>a|a195>g|t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|a225>g|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|c255>t|a258>c|g261>c|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| c87>g|a103>g,S35>G(+ + -)|a106>t|g107>c|c108>g| c120>t| a168>t|a169>c,K57>H(- - +)|g171>c,K57>H(- - +)|c172>g,Q58>E(+ + -)|t177>a,D59>E(+ - +)|g192>a|a195>g| t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|a225>g|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|c255>t|a258>c|g261>c|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| 320 226 201 25 13 12 4 3 6 0 2 0 2 2 3 3 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 4 1 1 0 0 0 0 0 1 0 1 2 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 17 7 3 4 1 1 0 0 1 0 1 1 1 1 0 0 117 114 102 12 5 7 2 2 5 0 1 0 0 1 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacgtttggttcgtattgg atgagttgggtccgccaggctccagggaaggggctggagtgggtggccaac attcacgaagaaggaagtgaaaag aattatgtggactctgtgaagggccggttcgtcatctccagagacaacgccaagaattccctctatctgcaaatgaacagcctgagagtcgaggacacggccatatattattgt gcgagagatcgatatagtagcgattccagttactacatgcccaactggctcgacccc ARDRYSSDSSYYMPNWLDP IGHV3-7 IGHD4-11 IGHJ5 ggattcacgtttggttcgtattgg atgagttgggtccgccaggctccagggaaggggctggagtgggtggccaac attcacgaagaaggaagtgaaaag aattatgtggactctgtgaagggccggttcgtcatctccagagacaacgccaagaattccctctatctgcaaatgaacagcctgagagtcgaggacacggccatatattattgt gcgagagatcgatatagtagcgattccagttactacatgcccaactggctcgacccc IGA1 25 213 13 12 9 15 4 10 6 7 7 5 JY8QFUQ01AOQZO 5941 productive (see comment) Homsap IGHV4-61*02 F 857 87.78 194/221 nt 87.33 193/221 nt Homsap IGHJ4*03 F 186 87.5 42/48 nt Homsap IGHD5-12*01 F 1 10 7 16 10.7.16 [1.17.38.11] CARERSASMGAARELDSW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 63 of V-REGION: 'GGG' (3 nucleotides at position 99 to 101 in the user submitted sequence), (do not cause frameshift); tctctggtggccccatcggcagtggtgcctactactggacctggatccggcagcccgccgggaagggattggagtggctcgggcgtgtttatagtggtGGGgggatcatcaattacagtccctccctcaagagtcgaatcaccatgtcgatagacacgtccaagaaccagttctccctgaagttgacctctgtgaccgccgcagacacggccttatattactgtgcgagagaacgtagtgcctcgatgggcgctgcgagagaacttgactcctggggccaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [303] t85>c,S29>P(+ - -)|a91>g,S31>G(+ + -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|t108>c,S36>A(- + -)|g119>c,S40>T(+ - +)|c148>t|a157>c,I53>L(+ + +)|t159>c,I53>L(+ + +)|a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|c173>g,T58>S(+ - +)|c174>t,T58>S(+ - +)|a175>g,S59>G(+ + -)|g191>t,S64>I(- - -)|c194>t,T65>I(- - -)|c198>t|a203>g,N68>S(- - -)|c204>t,N68>S(- - -)|g226>a,V76>I(+ - +)|a234>g,I78>M(+ + -)|a237>g|g238>a,V80>I(+ - +)|c271>t|g275>c,S92>T(+ - +)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)| t85>c,S29>P(+ - -)|a91>g,S31>G(+ + -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|t108>c,S36>A(- + -)| g119>c,S40>T(+ - +)|c148>t|a157>c,I53>L(+ + +)|t159>c,I53>L(+ + +)| a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|c173>g,T58>S(+ - +)|c174>t,T58>S(+ - +)|a175>g,S59>G(+ + -)|g191>t,S64>I(- - -)|c194>t,T65>I(- - -)| c198>t|a203>g,N68>S(- - -)|c204>t,N68>S(- - -)|g226>a,V76>I(+ - +)|a234>g,I78>M(+ + -)|a237>g|g238>a,V80>I(+ - +)|c271>t|g275>c,S92>T(+ - +)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)| 320 229 202 27 4 23 7 3 7 3 1 0 0 0 3 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 25 5 0 5 2 0 0 2 0 0 0 0 1 0 0 0 51 51 47 4 1 3 0 0 1 1 1 0 0 0 1 0 0 0 30 21 14 7 0 7 2 0 3 0 0 0 0 0 0 1 1 0 117 114 103 11 3 8 3 3 3 0 0 0 0 0 1 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggccccatcggcagtggtgcctactac tggacctggatccggcagcccgccgggaagggattggagtggctcgggcgt gtttatagtggtgggatcatc aattacagtccctccctcaagagtcgaatcaccatgtcgatagacacgtccaagaaccagttctccctgaagttgacctctgtgaccgccgcagacacggccttatattactgt gcgagagaacgtagtgcctcgatgggcgctgcgagagaacttgactcc ARERSASMGAARELDS IGHV4-61 IGHD5-12 IGHJ4 ggtggccccatcggcagtggtgcctactac tggacctggatccggcagcccgccgggaagggattggagtggctcgggcgt gtttatagtggtgggatcatc aattacagtccctccctcaagagtcgaatcaccatgtcgatagacacgtccaagaaccagttctccctgaagttgacctctgtgaccgccgcagacacggccttatattactgt gcgagagaacgtagtgcctcgatgggcgctgcgagagaacttgactcc IGA1 27 216 20 7 10 16 10 11 4 0 11 12 JY8QFUQ01AOR4M 14630 productive Homsap IGHV3-7*01 F 950 93.12 203/218 nt NA Homsap IGHJ5*02 F (see comment) 183 84.31 43/51 nt Homsap IGHD3-22*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSDFYSTGHW in-frame - Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) gctctggattcaacttcagaacctattggatgggctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagcacgatggaagtgacaagtactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcattgtttttgcaaatggacagcctgagagccgaggacacggctgtgtactactgtgcgattagcagtgatttctactcgacgggccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [282, 281] c74>g|c86>a,T29>N(- + -)|t90>c|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|a118>g,S40>G(+ + -)|a174>c,Q58>H(- + -)|g192>c,E64>D(+ - +)|a195>g|a232>g,I78>V(+ - +)|c259>t|a263>t,Y88>F(- + -)|c265>t|a274>g,N92>D(+ + -)|t306>c| c74>g| c86>a,T29>N(- + -)|t90>c|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)| a118>g,S40>G(+ + -)| a174>c,Q58>H(- + -)|g192>c,E64>D(+ - +)|a195>g| a232>g,I78>V(+ - +)|c259>t|a263>t,Y88>F(- + -)|c265>t|a274>g,N92>D(+ + -)|t306>c| g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 316 (320) 222 (226) 207 15 (19) 6 (8) 9 (11) 4 (5) 0 (1) 2 2 1 1 1 (2) 1 2 1 0 (1) 0 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 20 4 1 3 0 0 0 1 0 1 0 1 1 0 0 0 51 51 50 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 1 0 0 0 1 0 0 0 1 0 0 0 117 114 108 6 3 3 2 0 2 1 0 0 1 0 0 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gctct ggattcaacttcagaacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcacgatggaagtgacaag tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcattgtttttgcaaatggacagcctgagagccgaggacacggctgtgtactactgt gcgattagcagtgatttctactcgacgggccac AISSDFYSTGH IGHV3-7 IGHD3-22 IGHJ5 ggattcaacttcagaacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcacgatggaagtgacaag tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcattgtttttgcaaatggacagcctgagagccgaggacacggctgtgtactactgt gcgattagcagtgatttctactcgacgggccac IGA2 14 213 8 6 2 5 6 9 3 2 4 5 JY8QFUQ01AOY3W 7435 productive (see comment) Homsap IGHV3-48*03 F 858 89.25 191/214 nt 87.85 188/214 nt Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD3-3*01 F 2 7 8 13 7.8.13 [1.16.38.11] CARDKGWSGYGMDVW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 38 of V-REGION: 1 nucleotides (from position 28 in the user submitted sequence), (cause frameshift)in FR2-IMGT, from codon 41 of V-REGION: 1 nucleotides (from position 37 in the user submitted sequence), (cause frameshift)in FR2-IMGT, from codon 45 of V-REGION: 2 nucleotides (from position 46 in the user submitted sequence), (cause frameshift) cctctggattcaccttcagttcttatgcatgaactggtccgcctgtccaggcaaggggctggaatggctttcatttattggtaatactggtagtgtcatatactacgcagactctgtgaaggggcgattcaccatctccagagacaatgccaagaactcaatgtctctacaaatgagcagcctgagagccgaggacacggctctatattattgtgcgagagataaaggttggagcggatacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [284, 283] a106>t|g107>c|a114>c|a131>t,Q44>L(- - -)|g141>c|g153>a|g157>c,V53>L(+ - +)|a164>t,Y55>F(- + -)|c165>t,Y55>F(- + -)|a169>g,S57>G(+ + -)|g173>a,S58>N(- - -)|g176>c,S59>T(+ - +)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)|c222>g|c246>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|a275>g,N92>S(- - -)|g301>c,V101>L(+ - +)|t303>a,V101>L(+ - +)|c309>t| a106>t|g107>c|a114>c| a131>t,Q44>L(- - -)|g141>c|g153>a|g157>c,V53>L(+ - +)|a164>t,Y55>F(- + -)|c165>t,Y55>F(- + -)| a169>g,S57>G(+ + -)|g173>a,S58>N(- - -)|g176>c,S59>T(+ - +)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)| c222>g|c246>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|a275>g,N92>S(- - -)|g301>c,V101>L(+ - +)|t303>a,V101>L(+ - +)|c309>t| 320 222 199 23 9 14 3 3 4 0 2 1 3 1 5 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 20 3 3 0 0 0 0 0 1 0 1 0 1 0 0 0 51 48 42 6 2 4 0 1 1 0 0 0 2 0 2 0 0 0 30 24 19 5 0 5 2 1 1 0 0 0 0 0 1 0 0 0 117 114 105 9 4 5 1 1 2 0 1 1 0 1 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,113-114(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagttcttatgc atgaactggtccgcctgtccaggcaaggggctggaatggctttcattt attggtaatactggtagtgtcata tactacgcagactctgtgaaggggcgattcaccatctccagagacaatgccaagaactcaatgtctctacaaatgagcagcctgagagccgaggacacggctctatattattgt gcgagagataaaggttggagcggatacggtatggacgtc ARDKGWSGYGMDV IGHV3-48 IGHD3-3 IGHJ6 ggattcaccttcagttcttatgc atgaactggtccgcctgtccaggcaaggggctggaatggctttcattt attggtaatactggtagtgtcata tactacgcagactctgtgaaggggcgattcaccatctccagagacaatgccaagaactcaatgtctctacaaatgagcagcctgagagccgaggacacggctctatattattgt gcgagagataaaggttggagcggatacggtatggacgtc IGA2 23 209 10 13 7 14 3 9 6 3 9 5 JY8QFUQ01AP05X 15702 productive Homsap IGHV3-7*01 F 1022 96.79 211/218 nt NA Homsap IGHJ1*01 F (see comment) 152 76.92 40/52 nt Homsap IGHD2-21*02 F 2 8 8 10 8.8.10 [1.17.38.11] CSNGYGDFPRRW in-frame + Other possibilities: Homsap_IGHJ4*02 and Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) cgtctggattcaccttcaggagctattggatgagctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagcaagatggaggtgacaaattctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgttcgaatggttacggtgacttcccgcgtaggtggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [279] c75>g|t90>c|t105>g,S35>R(- - -)|a187>g,S63>G(+ + -)|g192>c,E64>D(+ - +)|a197>t,Y66>F(- + -)|g303>a|g313>t,A105>S(- + -)| c75>g| t90>c|t105>g,S35>R(- - -)| a187>g,S63>G(+ + -)|g192>c,E64>D(+ - +)| a197>t,Y66>F(- + -)|g303>a| g313>t,A105>S(- + -)|g317>a,R106>N(+ - -)|a318>t,R106>N(+ - -)|a320>g| 316 (320) 222 (226) 214 (215) 8 (11) 3 (4) 5 (7) 1 (2) 1 (2) 0 1 0 0 1 (2) 0 1 1 1 1 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 22 2 1 1 0 0 0 1 0 0 0 0 0 0 0 1 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 117 114 112 2 1 1 0 1 0 0 0 0 1 0 0 0 0 0 4 (8) 4 3 (4) 1 (4) 0 (1) 1 (3) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cgtct ggattcaccttcaggagctattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaggtgacaaa ttctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt tcgaatggttacggtgacttcccgcgtagg SNGYGDFPRR IGHV3-7 IGHD2-21 IGHJ1 ggattcaccttcaggagctattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaggtgacaaa ttctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt tcgaatggttacggtgacttcccgcgtagg IGA1 6 213 3 3 1 2 2 4 1 1 1 3 JY8QFUQ01AP0TZ 13193 productive Homsap IGHV3-9*01 F 958 93.52 202/216 nt NA Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD6-13*01 F 2 8 8 16 8.8.16 [1.17.38.11] CAKDAMGGAGDWAAFGIW in-frame + tctggcttcatctttgatgattatgccatgcactgggtccggcaagctccagggaagggcctggagtgggtctcaggtattagttggaatagcgctaccatagaatatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctggaaatcaacagtctgagacctgaagacacggccttatactactgcgcaaaagatgcgatgggaggagctggtgattgggctgcttttggtatctggggccaggggacaatggtcactgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgctggtccagggcttcttccc NA IGA1 93 95 [295] a81>c|c86>t,T29>I(- - -)|t186>c|g188>c,G63>A(- + -)|g191>c,S64>T(+ - +)|g197>a,G66>E(- - -)|c198>a,G66>E(- - -)|c268>g,Q90>E(+ + -)|g273>c,M91>I(+ + -)|g286>c,A96>P(- - -)|g291>a|g303>a|t306>c|t312>c| a81>c|c86>t,T29>I(- - -)| t186>c|g188>c,G63>A(- + -)|g191>c,S64>T(+ - +)| g197>a,G66>E(- - -)|c198>a,G66>E(- - -)|c268>g,Q90>E(+ + -)|g273>c,M91>I(+ + -)|g286>c,A96>P(- - -)|g291>a|g303>a|t306>c|t312>c| a322>g| 321 (322) 225 (226) 211 14 (15) 6 (7) 8 0 (1) 3 1 3 1 1 0 0 4 1 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 1 0 1 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 0 0 0 1 0 0 0 0 2 0 0 0 117 114 105 9 4 5 0 3 0 2 0 1 0 0 2 1 0 0 9 (10) 9 9 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| tct ggcttcatctttgatgattatgcc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaggt attagttggaatagcgctaccata gaatatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctggaaatcaacagtctgagacctgaagacacggccttatactactgc gcaaaagatgcgatgggaggagctggtgattgggctgcttttggtatc AKDAMGGAGDWAAFGI IGHV3-9 IGHD6-13 IGHJ3 ggcttcatctttgatgattatgcc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaggt attagttggaatagcgctaccata gaatatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctggaaatcaacagtctgagacctgaagacacggccttatactactgc gcaaaagatgcgatgggaggagctggtgattgggctgcttttggtatc IGA1 14 213 7 7 4 10 3 4 4 2 5 3 JY8QFUQ01AP21C 3193 productive (see comment) Homsap IGHV1-18*01 F 876 90.19 193/214 nt 89.25 191/214 nt Homsap IGHJ4*02 F 123 72.92 35/48 nt Homsap IGHD1-14*01 ORF 3 8 8 8 8.8.8 [0.17.38.11] CARDRPNEFW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 45 to 45 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 104 of V-REGION: 'T' (1 nucleotides at position 213 to 213 in the user submitted sequence), (cause frameshift); tggttacccctttaatacctatggtatcacctgggtgcgacaggCcccctggacacggccttgagtggatggggtggatcagcgttcacgatggcaacacaaactatgcacagaatctccagggcagagtcaccctgaccacagacacatctacgagcacagcctacatggaactgaggggtctcagatctgacgacacggccgtttattatTtgtgcgagagatcgcccgaatgaattctggggccagggaaccctggtcaccgtcttcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [272] a85>c,T29>P(+ + -)|c104>a,T35>N(- + -)|c105>t,T35>N(- + -)|g107>c,S36>T(+ - +)|g119>c,S40>T(+ - +)|a144>c,Q48>H(- + -)|g147>c|a162>g|c173>t,A58>V(+ - +)|t175>c,Y59>H(+ - -)|a184>g,N62>D(+ + -)|t189>c|g210>t,K70>N(+ - -)|a232>c,M78>L(+ + -)|c249>t|g270>a|a277>g,S93>G(+ + -)|c279>t,S93>G(+ + -)|g282>c|g303>t|c309>t| a85>c,T29>P(+ + -)|c104>a,T35>N(- + -)|c105>t,T35>N(- + -)|g107>c,S36>T(+ - +)| g119>c,S40>T(+ - +)|a144>c,Q48>H(- + -)|g147>c|a162>g| c173>t,A58>V(+ - +)|t175>c,Y59>H(+ - -)|a184>g,N62>D(+ + -)|t189>c| g210>t,K70>N(+ - -)|a232>c,M78>L(+ + -)|c249>t|g270>a|a277>g,S93>G(+ + -)|c279>t,S93>G(+ + -)|g282>c|g303>t|c309>t| 320 222 201 21 8 13 3 1 5 2 3 1 0 0 4 0 2 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 1 0 1 1 0 0 1 0 0 0 51 51 47 4 2 2 1 0 0 0 1 0 0 0 2 0 0 0 30 24 20 4 1 3 1 0 1 2 0 0 0 0 0 0 0 0 117 114 105 9 5 4 1 1 3 0 1 0 0 0 1 0 2 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|ta,175-176(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,5-6(FR1)|tt,75-76(FR1)|tt,81-82(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|tt,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,3-6(FR1)|agct,8-11(FR1)|agct,24-27(FR1)|ggct,72-75(FR1)|ggtt,79-82(CDR1)|agct,106-109(CDR1)|ggta,112-115(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)| agct,8-11(FR1)|agct,24-27(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|agct,209-212(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggttacccctttaatacctatggt atcacctgggtgcgacaggcccctggacacggccttgagtggatggggtgg atcagcgttcacgatggcaacaca aactatgcacagaatctccagggcagagtcaccctgaccacagacacatctacgagcacagcctacatggaactgaggggtctcagatctgacgacacggccgtttattattgt gcgagagatcgcccgaatgaattc ARDRPNEF IGHV1-18 IGHD1-14 IGHJ4 ggttacccctttaatacctatggt atcacctgggtgcgacaggcccctggacacggccttgagtggatggggtgg atcagcgttcacgatggcaacaca aactatgcacagaatctccagggcagagtcaccctgaccacagacacatctacgagcacagcctacatggaactgaggggtctcagatctgacgacacggccgtttattattgt gcgagagatcgcccgaatgaattc IGA1 21 213 11 10 6 13 5 8 7 1 6 7 JY8QFUQ01AP263 18143 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 909 91.24 198/217 nt NA Homsap IGHJ6*02 F (see comment) 184 77.42 48/62 nt Homsap IGHD3-22*01 F 2 8 8 16 8.8.16 [1.17.38.11] CAKENYFESSGYHGMNVW in-frame + Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) or Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ctctggattcacctttaccaactatgccatgacctgggtccgccaggctccagggaaggggctggaatgggtctcaactattagtaatcgtggtactggagtgtactacgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacggtgtatctgcaaatgaacagcctgagagccgaggacacggccctttattactgtgcgaaagagaattactttgagagtagtggttatcacggtatgaacgtctggggccaggggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [296] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|g153>a|g163>a,A55>T(- - -)|g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|a175>c,S59>R(- - -)|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|a190>g,S64>G(+ + -)|c192>a,S64>G(+ + -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|a195>g,T65>V(- - -)|g225>c|c259>g,L87>V(+ - +)|g301>c,V101>L(+ - +)|a303>t,V101>L(+ - +)| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|g153>a|g163>a,A55>T(- - -)| g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|a175>c,S59>R(- - -)|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|a190>g,S64>G(+ + -)|c192>a,S64>G(+ + -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|a195>g,T65>V(- - -)| g225>c|c259>g,L87>V(+ - +)|g301>c,V101>L(+ - +)|a303>t,V101>L(+ - +)| 320 225 206 19 2 17 3 6 1 0 1 1 1 0 5 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 51 51 48 3 1 2 0 2 0 0 0 0 0 0 1 0 0 0 30 24 14 10 0 10 3 3 1 0 1 1 0 0 1 0 0 0 117 114 110 4 1 3 0 0 0 0 0 0 1 0 2 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaccaactatgcc atgacctgggtccgccaggctccagggaaggggctggaatgggtctcaact attagtaatcgtggtactggagtg tactacgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacggtgtatctgcaaatgaacagcctgagagccgaggacacggccctttattactgt gcgaaagagaattactttgagagtagtggttatcacggtatgaacgtc AKENYFESSGYHGMNV IGHV3-23 IGHD3-22 IGHJ6 ggattcacctttaccaactatgcc atgacctgggtccgccaggctccagggaaggggctggaatgggtctcaact attagtaatcgtggtactggagtg tactacgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacggtgtatctgcaaatgaacagcctgagagccgaggacacggccctttattactgt gcgaaagagaattactttgagagtagtggttatcacggtatgaacgtc IGA1 19 213 10 9 7 14 3 5 2 0 5 12 JY8QFUQ01AP2Y1 11037 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 873 89.4 194/217 nt NA Homsap IGHJ4*01 F 204 91.67 44/48 nt Homsap IGHD3-22*01 F 2 8 8 18 8.8.18 [1.17.38.11] CARDGVRGVGAYYIYYFDLW in-frame + ctctggattcacctttggcctctatgccatgaactgggtccgccagactccagggaaggggctggagtggctcgcaactattagtggtagtggaagtagatcattttacgcagactccctgaagggccggttcaccatctccagagacaattccaagggcacggtatacctggaaatgaccaccctgagagccgaggacacggccgtatattactgtgcgagagatggggtccgtggcgttggtgcttactacatctactattttgacttatggggccaaggaaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [302] a103>g,S35>G(+ + -)|a106>c,S36>L(- - -)|g107>t,S36>L(- - -)|g119>a,S40>N(- - -)|g133>a,A45>T(- - -)|g157>c,V53>L(+ - +)|t160>g,S54>A(- + -)|g163>a,A55>T(- - -)|t186>a|g187>a,G63>S(+ + -)|c192>a,S64>R(- - -)|a193>t,T65>S(+ - +)|a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|g211>c,V71>L(+ - +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t264>c|c268>g,Q90>E(+ + -)|a275>c,N92>T(- + -)|g278>c,S93>T(+ - +)|a317>g,K106>R(+ + +)| a103>g,S35>G(+ + -)|a106>c,S36>L(- - -)|g107>t,S36>L(- - -)| g119>a,S40>N(- - -)|g133>a,A45>T(- - -)|g157>c,V53>L(+ - +)|t160>g,S54>A(- + -)|g163>a,A55>T(- - -)| t186>a|g187>a,G63>S(+ + -)|c192>a,S64>R(- - -)|a193>t,T65>S(+ - +)| a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|g211>c,V71>L(+ - +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t264>c|c268>g,Q90>E(+ + -)|a275>c,N92>T(- + -)|g278>c,S93>T(+ - +)| a317>g,K106>R(+ + +)| 320 225 201 24 2 22 4 5 1 1 2 1 2 1 3 2 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 0 0 0 1 0 0 0 0 0 1 0 51 51 46 5 0 5 0 3 0 0 0 0 0 0 1 0 0 1 30 24 20 4 1 3 0 1 0 0 0 1 1 1 0 0 0 0 117 114 103 11 1 10 2 1 1 1 1 0 1 0 2 2 0 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttggcctctatgcc atgaactgggtccgccagactccagggaaggggctggagtggctcgcaact attagtggtagtggaagtagatca ttttacgcagactccctgaagggccggttcaccatctccagagacaattccaagggcacggtatacctggaaatgaccaccctgagagccgaggacacggccgtatattactgt gcgagagatggggtccgtggcgttggtgcttactacatctactattttgactta ARDGVRGVGAYYIYYFDL IGHV3-23 IGHD3-22 IGHJ4 ggattcacctttggcctctatgcc atgaactgggtccgccagactccagggaaggggctggagtggctcgcaact attagtggtagtggaagtagatca ttttacgcagactccctgaagggccggttcaccatctccagagacaattccaagggcacggtatacctggaaatgaccaccctgagagccgaggacacggccgtatattactgt gcgagagatggggtccgtggcgttggtgcttactacatctactattttgactta IGA1 23 213 10 13 6 13 4 10 1 1 15 6 JY8QFUQ01AP3M5 15842 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD3-3*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARDCDSGECNFDYW in-frame + ctctggattcacctttaccacctccgccatggcctgggtccgccaggttccagggaaggggctggagtgggtctcatccataagtagtggtagtacttacatatatcacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaagacactttacctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagattgtgacagtggtgaatgtaactttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [288] c90>t|g104>c,S35>T(+ - +)|t105>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)|a118>g,N40>A(- - -)|a119>c,N40>A(- - -)|c134>t,A45>V(+ - +)|t168>a|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|c198>t|t199>c,Y67>H(+ - -)|c255>g,N85>K(+ - -)|t256>a,S86>T(+ - +)|g261>t|t264>c| c90>t|g104>c,S35>T(+ - +)|t105>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)| a118>g,N40>A(- - -)|a119>c,N40>A(- - -)|c134>t,A45>V(+ - +)| t168>a|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)| c198>t|t199>c,Y67>H(+ - -)|c255>g,N85>K(+ - -)|t256>a,S86>T(+ - +)|g261>t|t264>c| 320 225 205 20 5 15 3 0 3 4 2 0 0 2 4 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 16 8 1 7 1 0 1 2 1 0 0 0 3 0 0 0 51 51 48 3 0 3 1 0 1 0 1 0 0 0 0 0 0 0 30 24 21 3 1 2 1 0 0 0 0 0 0 1 1 0 0 0 117 114 108 6 3 3 0 0 1 2 0 0 0 1 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaccacctccgcc atggcctgggtccgccaggttccagggaaggggctggagtgggtctcatcc ataagtagtggtagtacttacata tatcacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaagacactttacctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagattgtgacagtggtgaatgtaactttgactac ARDCDSGECNFDY IGHV3-21 IGHD3-3 IGHJ4 ggattcacctttaccacctccgcc atggcctgggtccgccaggttccagggaaggggctggagtgggtctcatcc ataagtagtggtagtacttacata tatcacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaagacactttacctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagattgtgacagtggtgaatgtaactttgactac IGG1 20 213 10 10 3 9 7 11 3 2 6 9 JY8QFUQ01AP4KF 1932 productive (see comment) Homsap IGHV3-9*01 F 986 94.95 207/218 nt 94.04 205/218 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-6*01 F 1 8 8 15 8.8.15 [2.16.38.11] CAKSGTYSSSSGHFDLW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'G' (1 nucleotides at position 24 to 24 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 41 of V-REGION: 1 nucleotides (from position 39 in the user submitted sequence), (cause frameshift) gcctctggattcaactttaataaGttatgccatgcactggtccggcaagctccagggaagggcctggagtgggtctcaagtattagttggaataatggtaacacagactatgcgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgagaactgaggacacggccttatattactgtgcgaaatccgggacgtatagcagctcgtccggccactttgacctctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [296] c86>a,T29>N(- + -)|g103>a,D35>N(+ + -)|g106>a,D36>N(+ + -)|g163>a,G55>S(+ + -)|g185>a,S62>N(- - -)|g191>a,S64>N(- - -)|t194>c,I65>T(- - -)|g197>a,G66>D(- - -)|a206>g,D69>G(- - -)|g286>a,A96>T(- - -)|g303>a|a315>g| c86>a,T29>N(- + -)|g103>a,D35>N(+ + -)|g106>a,D36>N(+ + -)| g163>a,G55>S(+ + -)| g185>a,S62>N(- - -)|g191>a,S64>N(- - -)|t194>c,I65>T(- - -)| g197>a,G66>D(- - -)|a206>g,D69>G(- - -)|g286>a,A96>T(- - -)|g303>a| a315>g|g319>t,D107>S(- - -)|a320>c,D107>S(- - -)|t321>c,D107>S(- - -)|a322>g| 318 (322) 224 (228) 212 12 (16) 2 (3) 10 (13) 2 (3) 8 0 1 (2) 0 (1) 1 0 0 0 0 0 (1) 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 2 0 0 0 1 0 0 0 0 0 0 51 50 49 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 2 0 1 0 0 0 0 0 0 0 0 117 114 110 4 1 3 1 3 0 0 0 0 0 0 0 0 0 0 6 (10) 6 5 1 (5) 1 (2) 0 (3) 1 (2) 0 0 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcaactttaataattatgcc atgcactggtccggcaagctccagggaagggcctggagtgggtctcaagt attagttggaataatggtaacaca gactatgcgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgagaactgaggacacggccttatattactgt gcgaaatccgggacgtatagcagctcgtccggccactttgacctc AKSGTYSSSSGHFDL IGHV3-9 IGHD6-6 IGHJ4 ggattcaactttaataattatgcc atgcactggtccggcaagctccagggaagggcctggagtgggtctcaagt attagttggaataatggtaacaca gactatgcgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgagaactgaggacacggccttatattactgt gcgaaatccgggacgtatagcagctcgtccggccactttgacctc IGG2 11 212 10 1 8 9 2 2 1 0 4 6 JY8QFUQ01AP6HU 5052 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 828 87.1 189/217 nt NA Homsap IGHJ4*02 F (see comment) 159 81.25 39/48 nt Homsap IGHD6-19*01 F 1 8 8 12 8.8.12 [1.17.38.11] CAKLENSSGNEDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctgaattaacgtttgatatatatgccatgacttgggtccgccaggctccagggaaggggctggaatgggtctcaactattactggtagtggtgataatgcctactacgcagacttcgtgaagggccgattcgccatctccagagacaattccaagaacatgttgtatttgcaaatgaacaacctgcgaggcgaggacacggccgtttattactgtgcgaaactggaaaatagcagtgggaatgaagactcctggggccagggaaccctggtcaccgtctccacagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] g80>a,G27>E(- - -)|c84>a,F28>L(+ - -)|c87>g|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c105>t,S35>D(- - -)|g107>t,S36>I(- - -)|c108>a,S36>I(- - -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g153>a|g163>a,A55>T(- - -)|g170>c,S57>T(+ - +)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)|a193>g,T65>A(- - -)|a195>c,T65>A(- - -)|c209>t,S70>F(- - -)|g225>a|a229>g,T77>A(- - -)|c257>t,T86>M(- - -)|c259>t|c265>t|g278>a,S93>N(- - -)|a283>c|c287>g,A96>G(- + -)|a303>t| g80>a,G27>E(- - -)|c84>a,F28>L(+ - -)|c87>g|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c105>t,S35>D(- - -)|g107>t,S36>I(- - -)|c108>a,S36>I(- - -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g153>a|g163>a,A55>T(- - -)| g170>c,S57>T(+ - +)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)|a193>g,T65>A(- - -)|a195>c,T65>A(- - -)| c209>t,S70>F(- - -)|g225>a|a229>g,T77>A(- - -)|c257>t,T86>M(- - -)|c259>t|c265>t|g278>a,S93>N(- - -)|a283>c|c287>g,A96>G(- + -)|a303>t| g319>c|a320>t| 318 (320) 223 (225) 195 28 (30) 7 (9) 21 3 8 7 0 2 2 1 (2) 0 2 (3) 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 16 8 1 7 1 2 1 0 0 2 0 0 0 1 1 0 51 51 47 4 1 3 0 2 1 0 0 0 0 0 1 0 0 0 30 24 18 6 0 6 1 2 1 0 1 0 0 0 1 0 0 0 117 114 104 10 5 5 1 2 4 0 1 0 1 0 0 1 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gaattaacgtttgatatatatgcc atgacttgggtccgccaggctccagggaaggggctggaatgggtctcaact attactggtagtggtgataatgcc tactacgcagacttcgtgaagggccgattcgccatctccagagacaattccaagaacatgttgtatttgcaaatgaacaacctgcgaggcgaggacacggccgtttattactgt gcgaaactggaaaatagcagtgggaatgaagactcc AKLENSSGNEDS IGHV3-23 IGHD6-19 IGHJ4 gaattaacgtttgatatatatgcc atgacttgggtccgccaggctccagggaaggggctggaatgggtctcaact attactggtagtggtgataatgcc tactacgcagacttcgtgaagggccgattcgccatctccagagacaattccaagaacatgttgtatttgcaaatgaacaacctgcgaggcgaggacacggccgtttattactgt gcgaaactggaaaatagcagtgggaatgaagactcc IGA1 28 213 18 10 15 22 3 6 6 1 8 13 JY8QFUQ01AP72B 5605 productive (see comment) Homsap IGHV3-7*01 F (see comment) 742 82.87 179/216 nt 82.41 178/216 nt Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD2-15*01 F 1 8 7 15 8.7.15 [1.17.38.11] CARLKGLTHHDFYFDLW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (82,87% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR2-IMGT, from codon 63 of V-REGION: 1 nucleotides (from position 96 in the user submitted sequence), (cause frameshift) ctctggattcgtctttactaatcattggatgagttgggtccgccaggccacagggaaggggccggagtgggtggccaacatatccccagacggaatacgaaatattttggggactctgtgaggggccgattcagcgtctccagagacaacggcaagcagtcatcgtatctggaaatgaataccctgacagtcgatgacacggctgtatacttctgtgcgaggttgaaggggctgacacatcacgacttctacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [293] a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>c,Y37>H(+ - -)|c120>t|t135>c|c136>a,P46>T(+ + -)|t149>c,L50>P(- - -)|a169>t,K57>S(- - -)|a170>c,K57>S(- - -)|g171>c,K57>S(- - -)|a173>c,Q58>P(- - -)|t177>c|g190>a,E64>T(- - -)|a191>c,E64>T(- - -)|c198>t|a200>t,Y67>F(- + -)|t203>g,V68>G(- - -)|a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|a232>g,I78>V(+ - +)|c248>g,A83>G(- + -)|a253>c,N85>Q(+ - +)|c255>g,N85>Q(+ - +)|c259>t,L87>S(- - -)|t260>c,L87>S(- - -)|c268>g,Q90>E(+ + -)|c276>t|g278>c,S93>T(+ - +)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)|g303>a|t306>c|a308>t,Y103>F(- + -)| a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>c,Y37>H(+ - -)| c120>t|t135>c|c136>a,P46>T(+ + -)|t149>c,L50>P(- - -)| a169>t,K57>S(- - -)|a170>c,K57>S(- - -)|g171>c,K57>S(- - -)|a173>c,Q58>P(- - -)|t177>c|g190>a,E64>T(- - -)|a191>c,E64>T(- - -)| c198>t|a200>t,Y67>F(- + -)|t203>g,V68>G(- - -)|a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|a232>g,I78>V(+ - +)|c248>g,A83>G(- + -)|a253>c,N85>Q(+ - +)|c255>g,N85>Q(+ - +)|c259>t,L87>S(- - -)|t260>c,L87>S(- - -)|c268>g,Q90>E(+ + -)|c276>t|g278>c,S93>T(+ - +)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)|g303>a|t306>c|a308>t,Y103>F(- + -)| a318>g|g319>t|a320>t| 317 (320) 221 (224) 184 37 (40) 7 (10) 30 3 (4) 3 7 6 4 1 3 (4) 0 4 4 1 (2) 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 1 2 1 0 0 0 0 1 0 0 0 51 51 47 4 2 2 0 0 1 2 0 1 0 0 0 0 0 0 30 23 16 7 1 6 0 1 0 1 3 0 1 0 1 0 0 0 117 114 94 20 4 16 2 1 4 2 1 0 2 0 2 4 1 1 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcgtctttactaatcattgg atgagttgggtccgccaggccacagggaaggggccggagtgggtggccaac atatccccagacggaatacgaaa tattttggggactctgtgaggggccgattcagcgtctccagagacaacggcaagcagtcatcgtatctggaaatgaataccctgacagtcgatgacacggctgtatacttctgt gcgaggttgaaggggctgacacatcacgacttctacttcgatctc ARLKGLTHHDFYFDL IGHV3-7 IGHD2-15 IGHJ2 ggattcgtctttactaatcattgg atgagttgggtccgccaggccacagggaaggggccggagtgggtggccaac atatccccagacggaatacgaaa tattttggggactctgtgaggggccgattcagcgtctccagagacaacggcaagcagtcatcgtatctggaaatgaataccctgacagtcgatgacacggctgtatacttctgt gcgaggttgaaggggctgacacatcacgacttctacttcgatctc IGG2 37 212 19 18 10 20 9 17 6 1 18 12 JY8QFUQ01AP9Y7 15599 productive (see comment) Homsap IGHV1-46*01 F, or Homsap IGHV1-46*03 F 876 90.19 193/214 nt 89.72 192/214 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD2-15*01 F 2 8 8 14 8.8.14 [0.17.38.11] CATDRCTGGTCCPHYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 45 to 45 in the user submitted sequence), (cause frameshift); tggatacactttcaccagttattatatacactgggtgcgacaggCcccctggacaggggcttgagtggatgggagttgtcaaccctggtgctgaatacacactctacgcacagaagttccagggcagactcaccttgaccagggacacgtccacgagcacagtctacatggagttgagtagcctgagatctgaggacacggccctgtattactgtgcgacagataggtgtactggtggtacctgctgtccacattactggggccagggaaccctgatcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [289] c87>t|c108>t|c111>t|g117>a,M39>I(+ + -)|a144>g|a163>g,I55>V(+ - +)|a165>t,I55>V(+ - +)|a166>g,I56>V(+ - +)|a175>g,S59>G(+ + -)|g185>c,G62>A(- + -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a196>c,S66>L(- - -)|g197>t,S66>L(- - -)|g226>c,V76>L(+ - +)|a232>t,M78>L(+ + -)|c271>t|c276>t|g301>c,V101>L(+ - +)|g317>c,R106>T(- - -)| c87>t|c108>t|c111>t| g117>a,M39>I(+ + -)|a144>g|a163>g,I55>V(+ - +)|a165>t,I55>V(+ - +)| a166>g,I56>V(+ - +)|a175>g,S59>G(+ + -)|g185>c,G62>A(- + -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)| a196>c,S66>L(- - -)|g197>t,S66>L(- - -)|g226>c,V76>L(+ - +)|a232>t,M78>L(+ + -)|c271>t|c276>t|g301>c,V101>L(+ - +)| g317>c,R106>T(- - -)| 320 222 200 22 6 16 4 3 5 0 1 0 3 1 4 0 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 3 0 0 0 3 0 0 0 0 0 0 0 0 0 51 51 47 4 1 3 2 1 0 0 0 0 1 0 0 0 0 0 30 24 17 7 0 7 2 2 0 0 0 0 1 1 1 0 0 0 117 114 107 7 2 5 0 0 2 0 1 0 1 0 2 0 1 0 8 8 7 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,162-163(FR2)|ta,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,62-63(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,164-165(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggca,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|aacc,169-172(CDR2)|agct,196-199(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacactttcaccagttattat atacactgggtgcgacaggcccctggacaggggcttgagtggatgggagtt gtcaaccctggtgctgaatacaca ctctacgcacagaagttccagggcagactcaccttgaccagggacacgtccacgagcacagtctacatggagttgagtagcctgagatctgaggacacggccctgtattactgt gcgacagataggtgtactggtggtacctgctgtccacattac ATDRCTGGTCCPHY IGHV1-46 IGHD2-15 IGHJ4 ggatacactttcaccagttattat atacactgggtgcgacaggcccctggacaggggcttgagtggatgggagtt gtcaaccctggtgctgaatacaca ctctacgcacagaagttccagggcagactcaccttgaccagggacacgtccacgagcacagtctacatggagttgagtagcctgagatctgaggacacggccctgtattactgt gcgacagataggtgtactggtggtacctgctgtccacattac IGG2 21 213 12 9 8 12 4 9 3 3 8 7 JY8QFUQ01APECD 16464 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 1081 99.54 218/219 nt 98.63 216/219 nt Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD3-22*01 F 3 8 8 14 8.8.14 [2.17.38.11] CARDFFIGGYYGMDVW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'G' (1 nucleotides at position 16 to 16 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 43 of V-REGION: 'G' (1 nucleotides at position 45 to 45 in the user submitted sequence), (cause frameshift); gcctctggattcaccGttcagtagctatagcatgaactgggtccGgccaggctccagggaaggggctggagtgggtctcatccattagtagtagtagtagttacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagcagcctgagagccgaggacacggctgtgtattactgtgcgagagatttcttcataggaggctactacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [294] a275>g,N92>S(- - -)| a275>g,N92>S(- - -)| 320 227 226 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 113 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtagtagttacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagcagcctgagagccgaggacacggctgtgtattactgt gcgagagatttcttcataggaggctactacggtatggacgtc ARDFFIGGYYGMDV IGHV3-21 IGHD3-22 IGHJ6 ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtagtagttacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagcagcctgagagccgaggacacggctgtgtattactgt gcgagagatttcttcataggaggctactacggtatggacgtc IGA1 1 213 1 0 0 0 1 1 0 0 1 0 JY8QFUQ01APENB 7060 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 959 93.58 204/218 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-3*01 F 3 8 8 11 8.8.11 [1.17.38.11] CARFGPLTRPDYW in-frame - cctctggattcacctttaccaactatggcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtggtgataccacataccacgcagactccgtgcagggccgattcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagtcgaggacacggccgtttattactgtgcgagattcggcccacttactcgacctgactactggggccagggaactttggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [282] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)|c164>g,A55>G(- + -)|g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)|t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|g225>a|t246>c|g258>t|c279>t|c287>t,A96>V(+ - +)|a303>t| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)| c164>g,A55>G(- + -)| g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)| t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|g225>a|t246>c|g258>t|c279>t|c287>t,A96>V(+ - +)|a303>t| a317>g,K106>R(+ + +)|g319>t|a320>t| 316 (320) 222 (226) 208 (209) 14 (17) 5 (7) 9 (10) 0 (1) 3 2 2 1 0 1 (2) 0 2 2 1 (2) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 117 114 106 8 5 3 0 1 2 2 1 0 1 0 0 0 1 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgataccaca taccacgcagactccgtgcagggccgattcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagtcgaggacacggccgtttattactgt gcgagattcggcccacttactcgacctgactac ARFGPLTRPDY IGHV3-23 IGHD3-3 IGHJ4 ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgataccaca taccacgcagactccgtgcagggccgattcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagtcgaggacacggccgtttattactgt gcgagattcggcccacttactcgacctgactac IGA1 14 213 7 7 5 10 2 4 5 0 4 5 JY8QFUQ01APGRW 4804 productive Homsap IGHV3-7*01 F 936 92.63 201/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD6-19*01 F 1 8 8 10 8.8.10 [1.17.38.11] CASGSGWTAEYW in-frame + ctctgaattcaccgttagcagtcattggatgaattgggtccgccaggctccagggaaggggctggaatgggtggccaacataaaacaagatgcaagtgagaaaaactatgtggactctgtgaagggccgattcaccatctcccgagacaacgccaagaattcactgtatctgcaaatgaacagcctgagactcgaggacacggctgtgtatttctgtgcgagtggcagtggctggacagctgaatactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgccctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [279] g80>a,G27>E(- - -)|t88>g,F30>V(+ - -)|t105>c|c108>t|t109>c,Y37>H(+ - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g153>a|g171>a|g185>c,G62>A(- + -)|t196>a,Y66>N(- - -)|a238>c|c255>t|g286>c,A96>L(+ - +)|c287>t,A96>L(+ - +)|a308>t,Y103>F(- + -)| g80>a,G27>E(- - -)|t88>g,F30>V(+ - -)|t105>c|c108>t|t109>c,Y37>H(+ - -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g153>a| g171>a|g185>c,G62>A(- + -)| t196>a,Y66>N(- - -)|a238>c|c255>t|g286>c,A96>L(+ - +)|c287>t,A96>L(+ - +)|a308>t,Y103>F(- + -)| a318>t,R106>S(- - -)|a320>g| 317 (320) 222 (225) 206 (207) 16 (18) 6 (7) 10 (11) 0 (1) 4 4 2 1 0 1 (2) 1 2 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 1 1 2 0 0 0 0 0 0 0 1 51 51 48 3 1 2 0 2 1 0 0 0 0 0 0 0 0 0 30 24 22 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 117 114 108 6 2 4 0 0 2 0 1 0 1 1 1 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (1) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gaattcaccgttagcagtcattgg atgaattgggtccgccaggctccagggaaggggctggaatgggtggccaac ataaaacaagatgcaagtgagaaa aactatgtggactctgtgaagggccgattcaccatctcccgagacaacgccaagaattcactgtatctgcaaatgaacagcctgagactcgaggacacggctgtgtatttctgt gcgagtggcagtggctggacagctgaatac ASGSGWTAEY IGHV3-7 IGHD6-19 IGHJ4 gaattcaccgttagcagtcattgg atgaattgggtccgccaggctccagggaaggggctggaatgggtggccaac ataaaacaagatgcaagtgagaaa aactatgtggactctgtgaagggccgattcaccatctcccgagacaacgccaagaattcactgtatctgcaaatgaacagcctgagactcgaggacacggctgtgtatttctgt gcgagtggcagtggctggacagctgaatac IGG2 16 213 10 6 8 10 2 6 3 3 6 4 JY8QFUQ01APICN 16283 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 801 85.71 186/217 nt NA Homsap IGHJ6*02 F (see comment) 193 79.03 49/62 nt Homsap IGHD6-19*01 F 1 8 8 17 8.8.17 [1.17.38.11] CVRDSDSSGYARYDSMDVW in-frame + Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ctctggattcaacctcaatacctttggcatgaactgggtccgccaggcgccagggaagggactggagtgggtctcacacgtcaatcggggtagtactcacatatactacgcaggctcagtgaggggccggttcaccatctccagagacgacgccgggaactcagtctatctgcaaatgaatagcctgagagccgaggacacgggtttatattattgtgtgagagacagtgactcgtccggctacgcgaggtacgactctatggacgtctggggccaagggaccgcggtcaccgtctcatcagcctccagccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccggaaccggtgaccgggtgtcgtggaactc NA IGG1 79 81 [301] c86>a,T29>N(- + -)|t88>c,F30>L(+ - -)|g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|a112>g,S38>G(+ + -)|t135>g|g147>a|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>a,S57>N(- - -)|a172>c,S58>R(- - -)|t174>g,S58>R(- - -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)|a206>g,D69>G(- - -)|a215>g,K72>R(+ + +)|a225>g|a244>g,N82>D(+ + -)|a250>g,K84>G(- - -)|a251>g,K84>G(- - -)|c259>g,L87>V(+ - +)|g261>c,L87>V(+ - +)|c276>t|c299>g,A100>G(- + -)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|c309>t|c314>t,A105>V(+ - +)| c86>a,T29>N(- + -)|t88>c,F30>L(+ - -)|g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|a112>g,S38>G(+ + -)| t135>g|g147>a|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>a,S57>N(- - -)|a172>c,S58>R(- - -)|t174>g,S58>R(- - -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)| a206>g,D69>G(- - -)|a215>g,K72>R(+ + +)|a225>g|a244>g,N82>D(+ + -)|a250>g,K84>G(- - -)|a251>g,K84>G(- - -)|c259>g,L87>V(+ - +)|g261>c,L87>V(+ - +)|c276>t|c299>g,A100>G(- + -)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|c309>t| c314>t,A105>V(+ - +)| 320 225 193 32 5 27 9 4 3 4 1 2 1 0 3 2 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 1 0 1 0 1 1 0 1 0 0 0 51 51 47 4 2 2 0 1 0 1 0 1 0 0 0 0 0 1 30 24 16 8 0 8 2 1 0 2 1 0 0 0 1 0 0 1 117 114 101 13 3 10 6 1 2 0 0 0 0 0 1 2 1 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaacctcaatacctttggc atgaactgggtccgccaggcgccagggaagggactggagtgggtctcacac gtcaatcggggtagtactcacata tactacgcaggctcagtgaggggccggttcaccatctccagagacgacgccgggaactcagtctatctgcaaatgaatagcctgagagccgaggacacgggtttatattattgt gtgagagacagtgactcgtccggctacgcgaggtacgactctatggacgtc VRDSDSSGYARYDSMDV IGHV3-21 IGHD6-19 IGHJ6 ggattcaacctcaatacctttggc atgaactgggtccgccaggcgccagggaagggactggagtgggtctcacac gtcaatcggggtagtactcacata tactacgcaggctcagtgaggggccggttcaccatctccagagacgacgccgggaactcagtctatctgcaaatgaatagcctgagagccgaggacacgggtttatattattgt gtgagagacagtgactcgtccggctacgcgaggtacgactctatggacgtc IGG1 31 213 19 12 6 14 13 17 5 0 12 14 JY8QFUQ01APJMN 14435 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 841 87.96 190/216 nt 87.5 189/216 nt Homsap IGHJ6*02 F (see comment) 193 79.03 49/62 nt Homsap IGHD6-6*01 F 1 8 8 17 8.8.17 [1.17.38.11] CVRDSGPSGYARYESMDVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) in FR3-IMGT at codon 94 of V-REGION: 'T' (1 nucleotides at position 186 to 186 in the user submitted sequence), (cause frameshift); tctggattcagcctcacttcctatggcatgaactgggtccgccaggctccagggagggggctggagtgggtctcacacgttaatatgggtagtactcacatatactacgtaggctccgtgaggggccgattcaccatctccagagacgacgccaagaactcagtgtatctgcagatgaacaacctTgagagccgaggacacggctctatattactgtgtgagagatagtggcccgtccggctacgctaggtacgagtctatggacgtctggggccaagggaccgcggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [300] c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|g104>c,S35>T(+ - +)|a106>t|g107>c|a112>g,S38>G(+ + -)|a143>g,K48>R(+ + +)|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)|a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|g173>t,S58>M(- - -)|t174>g,S58>M(- - -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)|c203>t,A68>V(+ - +)|a206>g,D69>G(- - -)|a210>c|a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|c259>g,L87>V(+ - +)|a270>g|g278>a,S93>N(- - -)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)|c314>t,A105>V(+ - +)| c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|g104>c,S35>T(+ - +)|a106>t|g107>c|a112>g,S38>G(+ + -)| a143>g,K48>R(+ + +)|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)| a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|g173>t,S58>M(- - -)|t174>g,S58>M(- - -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)| c203>t,A68>V(+ - +)|a206>g,D69>G(- - -)|a210>c|a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|c259>g,L87>V(+ - +)|a270>g|g278>a,S93>N(- - -)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| c314>t,A105>V(+ - +)| 320 224 197 27 4 23 8 3 2 3 1 1 1 0 4 2 1 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 2 4 1 0 0 1 0 0 1 0 2 1 0 0 51 51 48 3 0 3 1 0 0 1 0 1 0 0 0 0 0 0 30 24 17 7 0 7 2 1 0 1 0 0 0 0 1 0 1 1 117 114 104 10 2 8 4 2 1 0 1 0 0 0 1 1 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcagcctcacttcctatggc atgaactgggtccgccaggctccagggagggggctggagtgggtctcacac gttaatatgggtagtactcacata tactacgtaggctccgtgaggggccgattcaccatctccagagacgacgccaagaactcagtgtatctgcagatgaacaacctgagagccgaggacacggctctatattactgt gtgagagatagtggcccgtccggctacgctaggtacgagtctatggacgtc VRDSGPSGYARYESMDV IGHV3-21 IGHD6-6 IGHJ6 ggattcagcctcacttcctatggc atgaactgggtccgccaggctccagggagggggctggagtgggtctcacac gttaatatgggtagtactcacata tactacgtaggctccgtgaggggccgattcaccatctccagagacgacgccaagaactcagtgtatctgcagatgaacaacctgagagccgaggacacggctctatattactgt gtgagagatagtggcccgtccggctacgctaggtacgagtctatggacgtc IGG2 26 213 15 11 4 12 11 14 2 2 11 11 JY8QFUQ01APK7S 13685 productive (see comment) Homsap IGHV4-34*06 F (see comment) 682 80.19 170/212 nt 79.72 169/212 nt Homsap IGHJ4*02 F (see comment) 168 83.33 40/48 nt Homsap IGHD2-15*01 F 2 7 7 14 7.7.14 [1.17.38.11] CSRGGQSAGWHSFEAW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (80,19% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT, from codon 38 of V-REGION: 2 nucleotides (from position 27 in the user submitted sequence), (cause frameshift) tccatggtgggtccctcagggttaccctggacctggatccgccacaccgcagagaagggactggagtggattggtcaaatcaatagtgatggaaggacaacctacaactcggccctcatgggtcgagtcaccatttcaacagacacatccaagaatcagttctcgctgactgtggtttctgttgtcgccgcggacacggcaatgtattattgttcgagaggaggccaaagtgctggctggcactcctttgaagcctggggccagggaacccaggtaatcgtcacctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacctagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 79 89 [286] t76>c,Y26>H(+ - -)|t88>c,F30>L(+ - -)|t105>g,S35>R(- - -)|g107>t,G36>V(- - -)|t109>a,Y37>T(+ - -)|a110>c,Y37>T(+ - -)|g119>c,S40>T(+ - +)|g132>c,Q44>H(- + -)|c133>a,P45>T(+ + -)|c136>g,P46>A(- - -)|g140>a,G47>E(- - -)|g147>a|g162>t|g163>c,E55>Q(+ + -)|c172>a,H58>S(+ - -)|a173>g,H58>S(+ - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|c192>g,S64>R(- - -)|c195>a|a197>c,N66>T(- + -)|c205>t,P69>S(+ - -)|t208>g,S70>A(- + -)|a215>t,K72>M(- + -)|a220>g,S74>G(+ + -)|a234>t|g238>a,V80>T(- - -)|t239>c,V80>T(- - -)|g246>a|c255>t|c264>g|a269>c,K90>T(- - -)|g270>t,K90>T(- - -)|c271>g,L91>V(+ - +)|g275>t,G92>V(- - -)|c276>t,G92>V(- - -)|g282>t|a283>g,T95>V(- - -)|c284>t,T95>V(- - -)|c300>a|g301>a,V101>M(+ - -)|c309>t| t76>c,Y26>H(+ - -)| t88>c,F30>L(+ - -)|t105>g,S35>R(- - -)|g107>t,G36>V(- - -)|t109>a,Y37>T(+ - -)|a110>c,Y37>T(+ - -)| g119>c,S40>T(+ - +)|g132>c,Q44>H(- + -)|c133>a,P45>T(+ + -)|c136>g,P46>A(- - -)|g140>a,G47>E(- - -)|g147>a|g162>t|g163>c,E55>Q(+ + -)| c172>a,H58>S(+ - -)|a173>g,H58>S(+ - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|c192>g,S64>R(- - -)|c195>a| a197>c,N66>T(- + -)|c205>t,P69>S(+ - -)|t208>g,S70>A(- + -)|a215>t,K72>M(- + -)|a220>g,S74>G(+ + -)|a234>t|g238>a,V80>T(- - -)|t239>c,V80>T(- - -)|g246>a|c255>t|c264>g|a269>c,K90>T(- - -)|g270>t,K90>T(- - -)|c271>g,L91>V(+ - +)|g275>t,G92>V(- - -)|c276>t,G92>V(- - -)|g282>t|a283>g,T95>V(- - -)|c284>t,T95>V(- - -)|c300>a|g301>a,V101>M(+ - -)|c309>t| 311 212 170 42 10 32 4 6 5 3 3 4 2 1 3 4 5 2 78 5 4 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 36 22 17 5 0 5 0 0 0 1 1 0 0 1 0 0 1 1 51 51 43 8 2 6 0 2 0 0 0 1 0 0 3 1 1 0 30 21 15 6 1 5 2 1 0 0 0 2 0 0 0 1 0 0 116 114 91 22 7 15 2 3 5 1 2 1 2 0 0 2 3 1 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|ggct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| tccat ggtgggtccctcagggttaccc tggacctggatccgccacaccgcagagaagggactggagtggattggtcaa atcaatagtgatggaaggaca acctacaactcggccctcatgggtcgagtcaccatttcaacagacacatccaagaatcagttctcgctgactgtggtttctgttgtcgccgcggacacggcaatgtattattgt tcgagaggaggccaaagtgctggctggcactcctttgaagcc SRGGQSAGWHSFEA IGHV4-34 IGHD2-15 IGHJ4 ggtgggtccctcagggttaccc tggacctggatccgccacaccgcagagaagggactggagtggattggtcaa atcaatagtgatggaaggaca acctacaactcggccctcatgggtcgagtcaccatttcaacagacacatccaagaatcagttctcgctgactgtggtttctgttgtcgccgcggacacggcaatgtattattgt tcgagaggaggccaaagtgctggctggcactcctttgaagcc IGA1 41 208 17 24 11 27 6 14 9 1 21 10 JY8QFUQ01APLRK 19037 productive (see comment) Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 859 88.89 192/216 nt 88.43 191/216 nt Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD5-18*01 F 3 8 7 21 8.7.21 [1.17.38.11] CAKDSRAGGYPNGYFYYSGLDVW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 63 of V-REGION: 1 nucleotides (from position 96 in the user submitted sequence), (cause frameshift) ctctggacgctccttgagaagctttggcatgcactgggtccgccaggctccaggcaagggactggagtgggtggcacttacttcgtatgacggaataggaaatattatgcagactccgtgaagggccgattcaccatctccagagacaactccaagaatacgttatttctgcaaatggacagtctgagagctgaggacacggctctttattactgtgcgaaagattcgcgagcgggtggttatccaaatggatacttctattactctggtctggacgtctggggccaagggacaatagtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [311] t82>c,F28>R(- - -)|t83>g,F28>R(- - -)|a85>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|t105>a,S35>R(- - -)|a110>t,Y37>F(- + -)|g147>a|g163>c,V55>L(+ - +)|t167>c,I56>T(- - -)|a168>t,I56>T(- - -)|a171>g|t177>c|a191>g,N64>R(+ - -)|t192>g,N64>R(+ - -)|c198>t|t246>c|c255>t|c259>t|g261>a|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)|g317>a,R106>K(+ + +)| t82>c,F28>R(- - -)|t83>g,F28>R(- - -)|a85>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|t105>a,S35>R(- - -)|a110>t,Y37>F(- + -)| g147>a|g163>c,V55>L(+ - +)| t167>c,I56>T(- - -)|a168>t,I56>T(- - -)|a171>g|t177>c|a191>g,N64>R(+ - -)|t192>g,N64>R(+ - -)| c198>t|t246>c|c255>t|c259>t|g261>a|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)| g317>a,R106>K(+ + +)| 320 224 199 25 9 16 3 3 4 4 0 0 4 1 2 1 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 0 0 1 0 0 2 1 0 1 0 1 51 51 49 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 30 23 17 6 2 4 2 0 0 2 0 0 1 0 0 0 0 1 117 114 104 10 6 4 1 1 4 1 0 0 1 0 1 0 1 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggacgctccttgagaagctttggc atgcactgggtccgccaggctccaggcaagggactggagtgggtggcactt acttcgtatgacggaataggaaa tattatgcagactccgtgaagggccgattcaccatctccagagacaactccaagaatacgttatttctgcaaatggacagtctgagagctgaggacacggctctttattactgt gcgaaagattcgcgagcgggtggttatccaaatggatacttctattactctggtctggacgtc AKDSRAGGYPNGYFYYSGLDV IGHV3-30 IGHD5-18 IGHJ6 ggacgctccttgagaagctttggc atgcactgggtccgccaggctccaggcaagggactggagtgggtggcactt acttcgtatgacggaataggaaa tattatgcagactccgtgaagggccgattcaccatctccagagacaactccaagaatacgttatttctgcaaatggacagtctgagagctgaggacacggctctttattactgt gcgaaagattcgcgagcgggtggttatccaaatggatacttctattactctggtctggacgtc IGG2 24 212 13 11 6 10 7 14 7 2 5 10 JY8QFUQ01APM60 14563 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 1034 98.14 211/215 nt NA Homsap IGHJ1*01 F 242 96.15 50/52 nt Homsap IGHD2-2*02 F 2 8 7 15 8.7.15 [1.17.38.11] CARGVGVSWHTEYFQHW in-frame + cctctgggttcaccgtcagtagcaactacatgagctgggtccgccaggctccagggaaggggctggagtgggtctcagttatttatagcggtggtaccacattctacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgcatcttcaaatgaacagcctgagagccgaggacacggccgtgtattactgtgcgagaggggtcggcgtcagctggcatactgaatacttccagcactggggccagggcaccctggtcatcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccaag NA IGA1 100 100 [291] g191>c,S64>T(+ - +)|a197>t,Y66>F(- + -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)| g191>c,S64>T(+ - +)| a197>t,Y66>F(- + -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)| a320>g| 319 (320) 222 (223) 218 4 (5) 0 (1) 4 0 (1) 0 0 1 0 0 1 0 1 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 20 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 117 114 111 3 0 3 0 0 0 1 0 0 1 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct gggttcaccgtcagtagcaactac atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagtt atttatagcggtggtaccaca ttctacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgcatcttcaaatgaacagcctgagagccgaggacacggccgtgtattactgt gcgagaggggtcggcgtcagctggcatactgaatacttccagcac ARGVGVSWHTEYFQH IGHV3-53 IGHD2-2 IGHJ1 gggttcaccgtcagtagcaactac atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagtt atttatagcggtggtaccaca ttctacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgcatcttcaaatgaacagcctgagagccgaggacacggccgtgtattactgt gcgagaggggtcggcgtcagctggcatactgaatacttccagcac IGA1 4 210 1 3 0 2 1 2 0 0 3 1 JY8QFUQ01APOZ1 16087 productive (see comment) Homsap IGHV3-7*01 F 798 85 187/220 nt 84.55 186/220 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD6-13*01 F 2 8 8 14 8.8.14 [2.17.38.11] CARERSAAGVVFFDFW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 52 of V-REGION: 'A' (1 nucleotides at position 71 to 71 in the user submitted sequence), (cause frameshift); agcctctggattcgagtttagtgcttattggatgacttgggtccgccaggctccaggaaaggggctggagAtgggtggccagtattaagaaagatggacatgagaagaattatttggactccgtcaaggagcgattcaccatatccagagacaacgccagggactcggtgtctttgcaaatgaacagcctgcgagtcgaggacacggctgtgtatttctgtgcgagagagagatcagcagctggagtcgtcttctttgacttttggggccagggagccccggtcaccgtctccagagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 100 100 [295] a85>g,T29>E(- - -)|c86>a,T29>E(- - -)|c87>g,T29>E(- - -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|c108>t,S36>A(- + -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g141>a|a164>g,N55>S(- - -)|c165>t,N55>S(- - -)|a168>t|c172>a,Q58>K(+ - -)|a187>c,S63>H(+ - -)|g188>a,S63>H(+ - -)|a195>g|t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|g202>t,V68>L(+ - +)|t210>c|g213>c|g221>a,G74>E(- - -)|c222>g,G74>E(- - -)|c234>a|a251>g,K84>R(+ + +)|a253>g,N85>D(+ + -)|a258>g|c259>g,L87>V(+ - +)|a263>c,Y88>S(+ - -)|c265>t|a283>c|c287>t,A96>V(+ - +)|a308>t,Y103>F(- + -)| a85>g,T29>E(- - -)|c86>a,T29>E(- - -)|c87>g,T29>E(- - -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|c108>t,S36>A(- + -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g141>a|a164>g,N55>S(- - -)|c165>t,N55>S(- - -)| a168>t|c172>a,Q58>K(+ - -)|a187>c,S63>H(+ - -)|g188>a,S63>H(+ - -)|a195>g| t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|g202>t,V68>L(+ - +)|t210>c|g213>c|g221>a,G74>E(- - -)|c222>g,G74>E(- - -)|c234>a|a251>g,K84>R(+ + +)|a253>g,N85>D(+ + -)|a258>g|c259>g,L87>V(+ - +)|a263>c,Y88>S(+ - -)|c265>t|a283>c|c287>t,A96>V(+ - +)|a308>t,Y103>F(- + -)| 320 228 195 33 9 24 7 3 6 1 3 3 2 1 3 3 1 0 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 2 0 1 0 0 1 0 0 1 1 0 0 51 51 46 5 1 4 1 1 2 0 0 0 0 0 1 0 0 0 30 24 19 5 2 3 1 1 0 0 1 1 1 0 0 0 0 0 117 114 97 17 6 11 3 1 3 1 2 1 1 1 1 2 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct ggattcgagtttagtgcttattgg atgacttgggtccgccaggctccaggaaaggggctggagtgggtggccagt attaagaaagatggacatgagaag aattatttggactccgtcaaggagcgattcaccatatccagagacaacgccagggactcggtgtctttgcaaatgaacagcctgcgagtcgaggacacggctgtgtatttctgt gcgagagagagatcagcagctggagtcgtcttctttgacttt ARERSAAGVVFFDF IGHV3-7 IGHD6-13 IGHJ4 ggattcgagtttagtgcttattgg atgacttgggtccgccaggctccaggaaaggggctggagtgggtggccagt attaagaaagatggacatgagaag aattatttggactccgtcaaggagcgattcaccatatccagagacaacgccagggactcggtgtctttgcaaatgaacagcctgcgagtcgaggacacggctgtgtatttctgt gcgagagagagatcagcagctggagtcgtcttctttgacttt IGG1 33 213 17 16 9 19 8 14 7 2 15 9 JY8QFUQ01APYPD 12261 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 959 93.58 204/218 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-3*01 F 1 8 8 10 8.8.10 [1.17.38.11] CARGGLEPVDYW in-frame + cctctggattcaccttcagtacatactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcccgtatcaatcctgatgggcgaatcacaaactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggattcggctgtgtactactgtgcaaggggtggtttggagcccgtagattattggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG2 100 100 [280] g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|a162>c|t168>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|g216>t,K72>N(+ - -)|c294>t|a295>t,T99>S(+ - +)|t306>c| g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)| a162>c| t168>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|g216>t,K72>N(+ - -)|c294>t|a295>t,T99>S(+ - +)|t306>c| a318>g|a320>g| 317 (320) 223 (226) 209 (210) 14 (16) 4 (6) 10 0 (2) 1 1 2 3 1 1 1 2 0 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 1 0 0 1 0 0 0 51 51 50 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 30 24 18 6 1 5 0 0 0 1 2 0 0 1 1 0 1 0 117 114 109 5 2 3 0 1 1 1 0 0 1 0 0 0 1 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtacatactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcccgt atcaatcctgatgggcgaatcaca aactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggattcggctgtgtactactgt gcaaggggtggtttggagcccgtagattat ARGGLEPVDY IGHV3-74 IGHD3-3 IGHJ4 ggattcaccttcagtacatactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcccgt atcaatcctgatgggcgaatcaca aactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggattcggctgtgtactactgt gcaaggggtggtttggagcccgtagattat IGG2 14 213 4 10 2 7 2 7 3 1 3 7 JY8QFUQ01AQ0K4 11478 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 896 90.37 197/218 nt 88.99 194/218 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [2.16.38.11] CVRAGFGELSGFDCW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'CT' (2 nucleotides at position 17 to 18 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 41 of V-REGION: 1 nucleotides (from position 39 in the user submitted sequence), (cause frameshift)in FR2-IMGT, from codon 45 of V-REGION: 1 nucleotides (from position 49 in the user submitted sequence), (cause frameshift) ggcctctggattcaccCTttgggttattatggcatggactggtccgccaactccgggggagggggctgagtgggtctctggtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [291] a72>g|t90>g,F30>L(+ - -)|a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a118>g,S40>D(- - -)|g119>a,S40>D(- - -)|a138>g|a142>g,K48>E(+ - -)|c148>g,L50>A(+ - +)|t149>c,L50>A(+ - +)|g150>t,L50>A(+ - +)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a72>g| t90>g,F30>L(+ - -)|a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a118>g,S40>D(- - -)|g119>a,S40>D(- - -)|a138>g|a142>g,K48>E(+ - -)|c148>g,L50>A(+ - +)|t149>c,L50>A(+ - +)|g150>t,L50>A(+ - +)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 225 (226) 203 22 (23) 4 (5) 18 5 4 1 2 1 (2) 0 2 0 2 2 2 1 78 7 6 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 0 0 0 0 0 0 0 0 0 1 1 51 49 42 7 1 6 3 1 0 1 0 0 0 0 0 1 1 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ggcctct ggattcaccttgggttattatggc atggactggtccgccaactccgggggagggggctgagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcaccttgggttattatggc atggactggtccgccaactccgggggagggggctgagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 20 211 10 10 4 10 6 10 3 0 12 5 JY8QFUQ01AQ18D 17673 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 984 93.72 209/223 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-16*01 F 2 8 10 14 8.10.14 [1.17.38.11] CTTDPRYFWGSGFDYW in-frame + ctctggattccctttcagcaacacctacatgagctgggtccgccaggctccagggaaggggctggagtgggttggccgtattaaaagcaaaactgatggtgggacaatagaatacgctgcacccgtgaaaggcagattaaccatatcaagagacgattcaaaaaacacgctgtatctgcatatgagcagcctgaagatcgaggacacagccgtgtattactgtaccaccgatccccgttacttttgggggagcgggtttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttct NA IGA1 100 100 [296] a85>c,T29>P(+ + -)|t105>c|g109>a,A37>T(- - -)|g113>a,W38>Y(- + -)|g114>c,W38>Y(- + -)|c194>t,T65>I(- - -)|c198>a,D66>E(+ - +)|c228>a,F76>L(+ - -)|c234>a|t243>c|a270>t,Q90>H(- + -)|a275>g,N92>S(- - -)|a285>g|c287>t,T96>I(- - -)|a318>c| a85>c,T29>P(+ + -)|t105>c|g109>a,A37>T(- - -)|g113>a,W38>Y(- + -)|g114>c,W38>Y(- + -)| c194>t,T65>I(- - -)| c198>a,D66>E(+ - +)|c228>a,F76>L(+ - -)|c234>a|t243>c|a270>t,Q90>H(- + -)|a275>g,N92>S(- - -)|a285>g|c287>t,T96>I(- - -)| a318>c| 320 231 216 15 5 10 2 2 2 2 2 3 1 0 1 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 2 0 1 1 0 0 0 1 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 29 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 117 114 106 8 3 5 2 0 1 1 0 3 1 0 0 0 0 0 8 8 7 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| ctct ggattccctttcagcaacacctac atgagctgggtccgccaggctccagggaaggggctggagtgggttggccgt attaaaagcaaaactgatggtgggacaata gaatacgctgcacccgtgaaaggcagattaaccatatcaagagacgattcaaaaaacacgctgtatctgcatatgagcagcctgaagatcgaggacacagccgtgtattactgt accaccgatccccgttacttttgggggagcgggtttgactac TTDPRYFWGSGFDY IGHV3-15 IGHD3-16 IGHJ4 ggattccctttcagcaacacctac atgagctgggtccgccaggctccagggaaggggctggagtgggttggccgt attaaaagcaaaactgatggtgggacaata gaatacgctgcacccgtgaaaggcagattaaccatatcaagagacgattcaaaaaacacgctgtatctgcatatgagcagcctgaagatcgaggacacagccgtgtattactgt accaccgatccccgttacttttgggggagcgggtttgactac IGA1 14 219 8 6 4 8 4 6 3 1 5 5 JY8QFUQ01AQ576 22201 productive Homsap IGHV3-7*01 F 977 94.5 206/218 nt NA Homsap IGHJ6*02 F 238 87.1 54/62 nt Homsap IGHD3-22*01 F 2 8 8 14 8.8.14 [1.17.38.11] CAKDGYYYYHSGMDVW in-frame + cctctggattcagatttagtaactactggatgagttgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagcaagatggaggtgagaagtattatgtgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattattgtgcgaaagacggctattattattatcacagcggtatggacgtctggggccaagggaccacggtcatcgtctccccagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccccag NA IGA1 100 100 [291] c86>g,T29>R(- - -)|c87>a,T29>R(- - -)|g107>a,S36>N(- - -)|t111>c|c120>t|a187>g,S63>G(+ + -)|a195>g|c198>t|a206>g,D69>G(- - -)|c259>g,L87>V(+ - +)|g303>a|c309>t|g317>a,R106>K(+ + +)| c86>g,T29>R(- - -)|c87>a,T29>R(- - -)|g107>a,S36>N(- - -)|t111>c| c120>t| a187>g,S63>G(+ + -)|a195>g| c198>t|a206>g,D69>G(- - -)|c259>g,L87>V(+ - +)|g303>a|c309>t| g317>a,R106>K(+ + +)| 320 226 213 13 6 7 3 3 3 1 0 1 0 0 0 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 0 1 0 1 0 0 0 1 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 22 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 117 114 109 5 3 2 1 1 2 0 0 0 0 0 0 1 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcagatttagtaactactgg atgagttgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaggtgagaag tattatgtgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattattgt gcgaaagacggctattattattatcacagcggtatggacgtc AKDGYYYYHSGMDV IGHV3-7 IGHD3-22 IGHJ6 ggattcagatttagtaactactgg atgagttgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaggtgagaag tattatgtgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattattgt gcgaaagacggctattattattatcacagcggtatggacgtc IGA1 12 213 9 3 5 8 4 4 4 2 2 4 JY8QFUQ01AQ8YC 12147 productive (see comment) Homsap IGHV3-11*01 F 833 87.16 190/218 nt 86.7 189/218 nt Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD1-7*01 F 1 8 8 15 8.8.15 [1.17.38.11] CVRESRSWTGSTLPAYW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 97 of V-REGION: 'T' (1 nucleotides at position 195 to 195 in the user submitted sequence), (cause frameshift); cctctggattcaccttcagtgactattacatgagttggtaccgccaggctccaggtcaggggctggagtggctttcatatataagtcaaactggcaaaaccacatacttcgcagactctgtgaagggccgattcaccatctccagggacaatgccaagacatcagtgtttctgcaaatgaacagcctgagagttTgacgacacggccgtgtatttttgtgtgagagaatctaggagttggactggaagtacacttccagcctactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 100 [295] c111>t|c120>t|a124>t,I42>Y(- - -)|t125>a,I42>Y(- - -)|g141>t|a142>c,K48>Q(+ - -)|g157>c,V53>L(+ - +)|c165>t|t168>a|a172>c,S58>Q(- - -)|g173>a,S58>Q(- - -)|t174>a,S58>Q(- - -)|g176>c,S59>T(+ - +)|t186>c|g188>a,S63>K(- - -)|t189>a,S63>K(- - -)|t194>c,I65>T(- - -)|a200>t,Y67>F(- + -)|c246>t|a254>c,N85>T(- + -)|c255>a,N85>T(- + -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|c288>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)|c314>t,A105>V(+ - +)| c111>t| c120>t|a124>t,I42>Y(- - -)|t125>a,I42>Y(- - -)|g141>t|a142>c,K48>Q(+ - -)|g157>c,V53>L(+ - +)|c165>t| t168>a|a172>c,S58>Q(- - -)|g173>a,S58>Q(- - -)|t174>a,S58>Q(- - -)|g176>c,S59>T(+ - +)|t186>c|g188>a,S63>K(- - -)|t189>a,S63>K(- - -)|t194>c,I65>T(- - -)| a200>t,Y67>F(- + -)|c246>t|a254>c,N85>T(- + -)|c255>a,N85>T(- + -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|c288>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| c314>t,A105>V(+ - +)| 320 226 197 29 7 22 0 2 8 2 3 1 4 4 3 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 44 7 3 4 0 0 2 0 1 0 1 1 1 0 1 0 30 24 15 9 2 7 0 2 0 2 1 0 0 3 1 0 0 0 117 114 103 11 1 10 0 0 4 0 1 1 3 0 1 1 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtgactattac atgagttggtaccgccaggctccaggtcaggggctggagtggctttcatat ataagtcaaactggcaaaaccaca tacttcgcagactctgtgaagggccgattcaccatctccagggacaatgccaagacatcagtgtttctgcaaatgaacagcctgagagttgacgacacggccgtgtatttttgt gtgagagaatctaggagttggactggaagtacacttccagcctac VRESRSWTGSTLPAY IGHV3-11 IGHD1-7 IGHJ4 ggattcaccttcagtgactattac atgagttggtaccgccaggctccaggtcaggggctggagtggctttcatat ataagtcaaactggcaaaaccaca tacttcgcagactctgtgaagggccgattcaccatctccagggacaatgccaagacatcagtgtttctgcaaatgaacagcctgagagttgacgacacggccgtgtatttttgt gtgagagaatctaggagttggactggaagtacacttccagcctac IGA1 28 213 11 17 9 15 2 13 4 3 14 7 JY8QFUQ01AQAEH 10415 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 945 93.09 202/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD3-10*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKKYHYGSGSYLYSFDYW in-frame + ctctgggttcacctttgcccactttgccatgacctgggtccgccaggctccagggaaggggctggagtgggtctcaactattagtggtggtgatgattccacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatggacagcctgagagccgaggacacggccgtatatcactgtgcgaagaagtatcactatggttcggggagttatctctactcctttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagcccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 100 95 [300, 299] a81>g|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a110>t,Y37>F(- + -)|g119>c,S40>T(+ - +)|g163>a,A55>T(- - -)|a175>g,S59>G(+ + -)|g185>a,G62>D(- - -)|g188>a,G63>D(- - -)|a190>t|g191>c|a274>g,N92>D(+ + -)|t307>c,Y103>H(+ - -)| a81>g|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a110>t,Y37>F(- + -)| g119>c,S40>T(+ - +)|g163>a,A55>T(- - -)| a175>g,S59>G(+ + -)|g185>a,G62>D(- - -)|g188>a,G63>D(- - -)|a190>t|g191>c| a274>g,N92>D(+ + -)|t307>c,Y103>H(+ - -)| a318>g|g319>a| 317 (320) 222 (225) 207 (208) 15 (17) 3 (5) 12 4 (5) 4 (5) 0 1 1 0 2 0 3 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 1 5 2 1 0 0 1 0 1 0 1 0 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 30 24 19 5 2 3 1 2 0 0 0 0 1 0 1 0 0 0 117 114 112 2 0 2 1 0 0 1 0 0 0 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcacctttgcccactttgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtggtggtgatgattccaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatggacagcctgagagccgaggacacggccgtatatcactgt gcgaagaagtatcactatggttcggggagttatctctactcctttgactac AKKYHYGSGSYLYSFDY IGHV3-23 IGHD3-10 IGHJ4 gggttcacctttgcccactttgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtggtggtgatgattccaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatggacagcctgagagccgaggacacggccgtatatcactgt gcgaagaagtatcactatggttcggggagttatctctactcctttgactac IGA2 15 213 9 6 4 7 5 8 0 3 4 8 JY8QFUQ01AQAXR 20488 productive Homsap IGHV3-30*01 F, or Homsap IGHV3-30*13 F 909 91.24 198/217 nt NA Homsap IGHJ6*02 F 184 77.42 48/62 nt Homsap IGHD3-10*01 F 2 8 8 18 8.8.18 [1.17.38.11] CARDDFYSSGNYGGFGMDVW in-frame + ctctggattcaccttcagtcactatggtatacactgggtccgccaggctccaggcaaggggctagattgggtggcatttatatcatatgatggaagtaatgagttttacgcagactccgtgaggggccgattcaccatctccagagacaactccaagaacatgatgtatctgcaaatgaacatcctgagacgtgaggacacggctctttattactgtgcgagagatgatttctattcttcggggaattacggaggcttcggaatggacgtctggggccgagggaccgcggtcagcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [302] a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|c113>g,A38>G(- + -)|g117>a,M39>I(+ + -)|g153>t,E51>D(+ - +)|g163>t,V55>F(+ - -)|a193>g,K65>E(+ - -)|a195>g,K65>E(+ - -)|a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|a215>g,K72>R(+ + +)|t246>c|c257>t,T86>M(- - -)|c259>a,L87>M(+ + -)|g278>t,S93>I(- - -)|g286>c,A96>R(- - -)|c287>g,A96>R(- - -)|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)| a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|c113>g,A38>G(- + -)| g117>a,M39>I(+ + -)|g153>t,E51>D(+ - +)|g163>t,V55>F(+ - -)| a193>g,K65>E(+ - -)|a195>g,K65>E(+ - -)| a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|a215>g,K72>R(+ + +)|t246>c|c257>t,T86>M(- - -)|c259>a,L87>M(+ + -)|g278>t,S93>I(- - -)|g286>c,A96>R(- - -)|c287>g,A96>R(- - -)|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)| 320 225 206 19 1 18 3 2 2 1 1 1 1 0 2 2 4 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 1 0 0 0 0 1 0 0 51 51 48 3 0 3 0 1 0 0 0 0 0 0 0 0 2 0 30 24 22 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 117 114 103 11 1 10 1 0 2 1 0 1 1 0 2 1 2 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtcactatggt atacactgggtccgccaggctccaggcaaggggctagattgggtggcattt atatcatatgatggaagtaatgag ttttacgcagactccgtgaggggccgattcaccatctccagagacaactccaagaacatgatgtatctgcaaatgaacatcctgagacgtgaggacacggctctttattactgt gcgagagatgatttctattcttcggggaattacggaggcttcggaatggacgtc ARDDFYSSGNYGGFGMDV IGHV3-30 IGHD3-10 IGHJ6 ggattcaccttcagtcactatggt atacactgggtccgccaggctccaggcaaggggctagattgggtggcattt atatcatatgatggaagtaatgag ttttacgcagactccgtgaggggccgattcaccatctccagagacaactccaagaacatgatgtatctgcaaatgaacatcctgagacgtgaggacacggctctttattactgt gcgagagatgatttctattcttcggggaattacggaggcttcggaatggacgtc IGA1 19 213 8 11 4 13 4 6 1 0 13 5 JY8QFUQ01AQMNE 12363 productive (see comment) Homsap IGHV3-66*02 F 907 92.02 196/213 nt 90.61 193/213 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-15*01 F 3 8 7 16 8.7.16 [2.16.38.11] CAKLADTGEWSNRIFYFW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 40 of V-REGION: 1 nucleotides (from position 35 in the user submitted sequence), (cause frameshift)in FR2-IMGT, from codon 45 of V-REGION: 1 nucleotides (from position 48 in the user submitted sequence), (cause frameshift)in FR2-IMGT, from codon 47 of V-REGION: 1 nucleotides (from position 53 in the user submitted sequence), (cause frameshift) gcctctggattcaccgtcagtagcagcttcatgacttggtccgccagctccaggaaaggactggagtgggtctcagtgctttatgtcggtggtaacacatactacgcagactccgtgaagggccgattcaccacctccagagacaattccgagaacactctgtatcttcaaatgaacaacctgagacctgaggactcggctgtgtattattgtgcgaaattggcggatactggagagtggagcaaccgcatcttttatttctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [293] a110>g,N37>S(- - -)|a113>t,Y38>F(- + -)|g122>t,W41>L(+ - -)|g144>a|g147>a|t165>g|a166>c,I56>L(+ + +)|a172>g,S58>V(- - -)|g173>t,S58>V(- - -)|g191>a,S64>N(- - -)|t233>c,I78>T(- - -)|a250>g,K84>E(+ - -)|g258>t|g278>a,S93>N(- - -)|g286>c,A96>P(- - -)|a295>t,T99>S(+ - +)|c309>t| a110>g,N37>S(- - -)|a113>t,Y38>F(- + -)| g122>t,W41>L(+ - -)|g144>a|g147>a|t165>g| a166>c,I56>L(+ + +)|a172>g,S58>V(- - -)|g173>t,S58>V(- - -)|g191>a,S64>N(- - -)| t233>c,I78>T(- - -)|a250>g,K84>E(+ - -)|g258>t|g278>a,S93>N(- - -)|g286>c,A96>P(- - -)|a295>t,T99>S(+ - +)|c309>t| g317>a,R106>K(+ + +)| 316 (318) 217 (219) 200 (201) 17 (18) 5 12 (13) 3 4 (5) 1 1 1 0 2 0 1 0 3 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 51 48 44 4 3 1 0 2 0 0 0 0 0 0 0 0 1 1 30 21 17 4 0 4 1 1 0 0 1 0 0 0 0 0 1 0 117 114 107 7 2 5 1 1 1 1 0 0 1 0 1 0 1 0 4 (6) 4 4 (5) 0 (1) 0 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcaccgtcagtagcagcttc atgacttggtccgccagctccaggaaaggactggagtgggtctcagtg ctttatgtcggtggtaacaca tactacgcagactccgtgaagggccgattcaccacctccagagacaattccgagaacactctgtatcttcaaatgaacaacctgagacctgaggactcggctgtgtattattgt gcgaaattggcggatactggagagtggagcaaccgcatcttttatttc AKLADTGEWSNRIFYF IGHV3-66 IGHD2-15 IGHJ4 ggattcaccgtcagtagcagcttc atgacttggtccgccagctccaggaaaggactggagtgggtctcagtg ctttatgtcggtggtaacaca tactacgcagactccgtgaagggccgattcaccacctccagagacaattccgagaacactctgtatcttcaaatgaacaacctgagacctgaggactcggctgtgtattattgt gcgaaattggcggatactggagagtggagcaaccgcatcttttatttc IGG2 17 207 9 8 5 9 4 8 5 0 6 6 JY8QFUQ01AQOEM 13151 productive Homsap IGHV3-48*02 F 896 90.37 197/218 nt NA Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 187 86 43/50 nt Homsap IGHD1-1*01 F 3 8 8 10 8.8.10 [1.17.38.11] CARDHIWAFDFW in-frame + cctctggattcaccttcagtgactatagcatgaactgggtccgccagactccagggaagggggtggagtggatttcatacatcggccgtggtggtgatgggatatactacgcagactctgtgaagggccgaatcaccatctccagagacaatgccaagaactcactttttctgcaaatgaacaccctgagagacgacgacacggctgtgtattactgtgcgagagaccacatttgggcttttgatttctggggccaagggacaaaggttaccgtctcgtcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [279] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g133>a,A45>T(- - -)|c148>g,L50>V(+ - +)|g157>a,V53>I(+ - +)|t168>c|a169>g,S57>G(+ + -)|t171>c,S57>G(+ + -)|a172>c,S58>R(- - -)|a175>g,S59>G(+ + -)|a184>g,S62>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a190>g,T64>G(+ - -)|c191>g,T64>G(+ - -)|c192>g,T64>G(+ - -)|t226>a,F76>I(+ - -)|g261>t|a263>t,Y88>F(- + -)|g278>c,S93>T(+ - +)|g291>c,E97>D(+ - +)| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g133>a,A45>T(- - -)|c148>g,L50>V(+ - +)|g157>a,V53>I(+ - +)| t168>c|a169>g,S57>G(+ + -)|t171>c,S57>G(+ + -)|a172>c,S58>R(- - -)|a175>g,S59>G(+ + -)|a184>g,S62>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a190>g,T64>G(+ - -)|c191>g,T64>G(+ - -)|c192>g,T64>G(+ - -)| t226>a,F76>I(+ - -)|g261>t|a263>t,Y88>F(- + -)|g278>c,S93>T(+ - +)|g291>c,E97>D(+ - +)| 320 226 205 21 2 19 6 4 0 2 1 0 1 1 2 3 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 48 3 0 3 0 2 0 0 0 0 0 0 0 1 0 0 30 24 13 11 1 10 5 1 0 2 1 0 0 0 0 2 0 0 117 114 109 5 1 4 0 0 0 0 0 0 1 1 2 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtgactatagc atgaactgggtccgccagactccagggaagggggtggagtggatttcatac atcggccgtggtggtgatgggata tactacgcagactctgtgaagggccgaatcaccatctccagagacaatgccaagaactcactttttctgcaaatgaacaccctgagagacgacgacacggctgtgtattactgt gcgagagaccacatttgggcttttgatttc ARDHIWAFDF IGHV3-48 IGHD1-1 IGHJ3 ggattcaccttcagtgactatagc atgaactgggtccgccagactccagggaagggggtggagtggatttcatac atcggccgtggtggtgatgggata tactacgcagactctgtgaagggccgaatcaccatctccagagacaatgccaagaactcactttttctgcaaatgaacaccctgagagacgacgacacggctgtgtattactgt gcgagagaccacatttgggcttttgatttc IGA2 21 213 12 9 4 10 8 11 1 1 7 12 JY8QFUQ01AQS9S 4681 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 838 87.21 191/219 nt 86.76 190/219 nt Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD1-26*01 F 3 7 8 10 7.8.10 [2.17.38.11] CTRDIGGRYSYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 30 of V-REGION: 1 nucleotides (from position 19 in the user submitted sequence), (cause frameshift) cctccgggagttcaccttagtagtttctggatgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacggactaatgagtatgggagtatcacaaactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgtacaagggacatcggtgggcgctactcctactggggccagggagtcctggtctccgtctcatcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 79 95 [280, 279] a72>c|g73>c,A25>L(+ - +)|c74>t,A25>L(+ - +)|t76>c,S26>R(- - -)|c77>g,S26>R(- - -)|t78>g,S26>R(- - -)|g80>a,G27>E(- - -)|a81>g,G27>E(- - -)|c108>t|a110>t,Y37>F(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g247>a,A83>T(- - -)|c257>g,T86>R(- - -)|g261>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|g313>a,A105>T(- - -)|a318>g| a72>c|g73>c,A25>L(+ - +)|c74>t,A25>L(+ - +)|t76>c,S26>R(- - -)|c77>g,S26>R(- - -)|t78>g,S26>R(- - -)| g80>a,G27>E(- - -)|a81>g,G27>E(- - -)|c108>t|a110>t,Y37>F(- + -)| c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g247>a,A83>T(- - -)|c257>g,T86>R(- - -)|g261>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| g313>a,A105>T(- - -)|a318>g| 320 227 197 30 6 24 5 8 3 2 1 0 1 0 1 2 3 4 78 7 1 6 1 5 0 0 1 1 1 0 0 0 1 1 0 1 36 23 19 4 1 3 1 1 1 0 0 0 1 0 0 0 0 0 51 51 46 5 2 3 1 1 1 0 0 0 0 0 0 0 0 2 30 24 18 6 0 6 1 1 0 1 0 0 0 0 0 0 2 1 117 114 107 7 1 6 1 4 0 0 0 0 0 0 0 1 1 0 8 8 6 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctccgg gagttcaccttagtagtttctgg atgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgt acaagggacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 gagttcaccttagtagtttctgg atgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgt acaagggacatcggtgggcgctactcctac IGA2 22 212 14 8 9 13 5 9 3 1 9 9 JY8QFUQ01AQUPN 3571 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 1006 97.18 207/213 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD5-12*01 F 1 8 8 11 8.8.11 [X.17.38.11] CVVQILGATIDYW in-frame + ggattcaccttcaatacctatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattagtagtggtagtacttacataaagtacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgtggtccagatcctaggggctacgattgactactggggccagggaacccgggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [277] g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)| g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)| a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)| t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)| c314>t,A105>V(+ - +)|a316>g,R106>V(- - -)|g317>t,R106>V(- - -)|a318>c,R106>V(- - -)|g319>c| 313 (320) 214 (221) 208 (210) 6 (11) 0 (1) 6 (10) 1 (2) 1 0 (1) 0 0 (1) 0 0 1 2 (3) 1 0 (1) 0 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 117 114 112 2 0 2 0 0 0 0 0 0 0 1 0 1 0 0 1 (8) 1 1 (3) 0 (5) 0 (1) 0 (4) 0 (1) 0 0 (1) 0 0 (1) 0 0 0 0 (1) 0 0 (1) 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ggattcaccttcaatacctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtggtagtacttacata aagtacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gtggtccagatcctaggggctacgattgactac VVQILGATIDY IGHV3-21 IGHD5-12 IGHJ4 ggattcaccttcaatacctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtggtagtacttacata aagtacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gtggtccagatcctaggggctacgattgactac IGA1 6 213 2 4 1 4 1 2 0 0 2 4 JY8QFUQ01AQWBS 9516 productive (see comment) Homsap IGHV4-39*02 F 978 94.09 207/220 nt 93.64 206/220 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-22*01 F 3 10 7 10 10.7.10 [1.16.38.11] CARIVVRQFDQW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 45 of V-REGION: 1 nucleotides (from position 56 in the user submitted sequence), (cause frameshift) tctctggtgactccatttccagtactagttattactggggctgggtccgccagccccagggaaggggctggagtggattgggggtatctattctagtgggaccacctactacaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaacaactacttctccctgaagctgagttctgtgaccgccgcagacacggctgtgtattactgtgcgagaatagtggttagacagtttgaccagtggggccagggaatcctggtcaccgtctcctcacatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [280] g83>a,G28>D(- - -)|c90>t|a91>t|g92>c|g104>c,S35>T(+ - +)|c111>t|a124>g,I42>V(+ - +)|a163>g,S55>G(+ + -)|a173>c,Y58>S(+ - -)|g191>c,S64>T(+ - +)|g252>c,K84>N(+ - -)|c256>t,H86>Y(+ - -)|c276>t| g83>a,G28>D(- - -)|c90>t|a91>t|g92>c|g104>c,S35>T(+ - +)|c111>t| a124>g,I42>V(+ - +)|a163>g,S55>G(+ + -)| a173>c,Y58>S(+ - -)|g191>c,S64>T(+ - +)| g252>c,K84>N(+ - -)|c256>t,H86>Y(+ - -)|c276>t| g319>a|a320>t| 318 (320) 226 (228) 213 13 (15) 5 (7) 8 2 1 (2) 4 0 1 0 1 (2) 0 4 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 4 2 0 1 2 0 0 0 1 0 2 0 0 0 51 50 48 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 30 21 19 2 0 2 0 0 0 0 1 0 0 0 1 0 0 0 117 114 111 3 1 2 0 0 2 0 0 0 0 0 1 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatttccagtactagttattac tggggctgggtccgccagccccagggaaggggctggagtggattgggggt atctattctagtgggaccacc tactacaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaacaactacttctccctgaagctgagttctgtgaccgccgcagacacggctgtgtattactgt gcgagaatagtggttagacagtttgaccag ARIVVRQFDQ IGHV4-39 IGHD3-22 IGHJ4 ggtgactccatttccagtactagttattac tggggctgggtccgccagccccagggaaggggctggagtggattgggggt atctattctagtgggaccacc tactacaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaacaactacttctccctgaagctgagttctgtgaccgccgcagacacggctgtgtattactgt gcgagaatagtggttagacagtttgaccag IGA1 13 215 7 6 5 9 2 4 1 4 4 4 JY8QFUQ01AQXAS 18775 productive Homsap IGHV4-61*02 F (see comment) 767 83.26 184/221 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-3*01 F 3 10 7 16 10.7.16 [1.17.38.11] CARERSISIGGAREFDSW in-frame - Low V-REGION identity (83,26% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tcactggtggccccatgggcagtagtttatacttctggaattgggtccgacagcccgccgggaagagactggagtggatcggacgtgtttatgatgatgggagtacccgctacaatccctccctcaggggtcgagtcaccatgtcagtagacacgtccaagaaccagttctccctgaggttgagttttgtgaccgccgcagacacggcccgttattactgtgcgagagaacggagtatttcgattggcggagcgagagaatttgactcttggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [300] t76>a,S26>T(+ - +)|t85>c,S29>P(+ - -)|c90>g,I30>M(+ + -)|a91>g,S31>G(+ + -)|g103>a,G35>S(+ + -)|a106>t,S36>L(- - -)|g107>t,S36>L(- - -)|t108>a,S36>L(- - -)|a113>t,Y38>F(- + -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a124>g,I42>V(+ - +)|g129>a|g145>a,G49>R(- - -)|t159>c|g162>a|a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|a172>g,T58>D(- + -)|c173>a,T58>D(- + -)|c174>t,T58>D(- + -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|c192>t|a196>c,N66>R(+ - -)|a197>g,N66>R(+ - -)|c204>t|a215>g,K72>R(+ + +)|a220>g,S74>G(+ + -)|a234>g,I78>M(+ + -)|a269>g,K90>R(+ + +)|c271>t|c276>t|c278>t,S93>F(- - -)|g301>c,V101>R(- - -)|t302>g,V101>R(- - -)|g303>t,V101>R(- - -)| t76>a,S26>T(+ - +)| t85>c,S29>P(+ - -)|c90>g,I30>M(+ + -)|a91>g,S31>G(+ + -)|g103>a,G35>S(+ + -)|a106>t,S36>L(- - -)|g107>t,S36>L(- - -)|t108>a,S36>L(- - -)|a113>t,Y38>F(- + -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a124>g,I42>V(+ - +)|g129>a|g145>a,G49>R(- - -)|t159>c|g162>a| a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|a172>g,T58>D(- + -)|c173>a,T58>D(- + -)|c174>t,T58>D(- + -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|c192>t| a196>c,N66>R(+ - -)|a197>g,N66>R(+ - -)|c204>t|a215>g,K72>R(+ + +)|a220>g,S74>G(+ + -)|a234>g,I78>M(+ + -)|a269>g,K90>R(+ + +)|c271>t|c276>t|c278>t,S93>F(- - -)|g301>c,V101>R(- - -)|t302>g,V101>R(- - -)|g303>t,V101>R(- - -)| 320 229 192 37 7 30 10 6 8 2 1 1 2 2 1 1 2 1 78 5 4 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 36 30 22 8 0 8 1 1 0 1 0 0 2 1 0 1 1 0 51 51 44 7 3 4 1 4 1 1 0 0 0 0 0 0 0 0 30 21 13 8 1 7 3 1 3 0 0 1 0 0 0 0 0 0 117 114 101 13 3 10 5 0 4 0 1 0 0 0 1 0 1 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tcact ggtggccccatgggcagtagtttatacttc tggaattgggtccgacagcccgccgggaagagactggagtggatcggacgt gtttatgatgatgggagtacc cgctacaatccctccctcaggggtcgagtcaccatgtcagtagacacgtccaagaaccagttctccctgaggttgagttttgtgaccgccgcagacacggcccgttattactgt gcgagagaacggagtatttcgattggcggagcgagagaatttgactct ARERSISIGGAREFDS IGHV4-61 IGHD3-3 IGHJ4 ggtggccccatgggcagtagtttatacttc tggaattgggtccgacagcccgccgggaagagactggagtggatcggacgt gtttatgatgatgggagtacc cgctacaatccctccctcaggggtcgagtcaccatgtcagtagacacgtccaagaaccagttctccctgaggttgagttttgtgaccgccgcagacacggcccgttattactgt gcgagagaacggagtatttcgattggcggagcgagagaatttgactct IGA1 36 216 26 10 14 19 12 17 6 1 14 15 JY8QFUQ01AQXWF 4935 productive Homsap IGHV4-34*10 F 813 86.92 186/214 nt NA Homsap IGHJ4*02 F 240 100 48/48 nt Homsap IGHD4-23*01 ORF 2 8 7 16 8.7.16 [1.17.38.11] CARNTNYGGNSGDYFDYW in-frame + gtctggtgagtccttcactaattactactggagctggatccgccagtccccaggaaagggtctggagtggcttggggaggtccatcatagtggacgcaccgactacaacccgtccctcaagagtcgaatcaccatgtcgttagacacgtccgaaaatcagttctccctgaagttgacttctttgaccgccgcggacacggcagtatattattgtgcgagaaataccaactatggtggcaattccggggactactttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [294] c75>g|a77>c,Y26>S(+ - -)|g83>a,G28>E(- - -)|g104>c,S35>T(+ - +)|g106>a,G36>N(- - -)|g107>a,G36>N(- - -)|c133>t,P45>S(+ - -)|g141>a|a147>t|a157>c,I53>L(+ + +)|a165>g|a166>g,I56>V(+ - +)|a169>c,N57>H(- - -)|a190>c,S64>R(- - -)|a196>g,N66>D(+ + -)|a237>g|g238>t,V80>L(+ - +)|a250>g,K84>E(+ - -)|g252>a,K84>E(+ - -)|c255>t|a263>c,Y88>S(+ - -)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g280>t,V94>L(+ - +)|c300>a|g303>a|c309>t| c75>g|a77>c,Y26>S(+ - -)| g83>a,G28>E(- - -)|g104>c,S35>T(+ - +)|g106>a,G36>N(- - -)|g107>a,G36>N(- - -)| c133>t,P45>S(+ - -)|g141>a|a147>t|a157>c,I53>L(+ + +)|a165>g| a166>g,I56>V(+ - +)|a169>c,N57>H(- - -)|a190>c,S64>R(- - -)| a196>g,N66>D(+ + -)|a237>g|g238>t,V80>L(+ - +)|a250>g,K84>E(+ - -)|g252>a,K84>E(+ - -)|c255>t|a263>c,Y88>S(+ - -)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g280>t,V94>L(+ - +)|c300>a|g303>a|c309>t| t319>a| 318 (320) 220 (222) 192 (193) 28 (29) 10 (11) 18 5 6 5 0 5 1 1 0 (1) 2 1 2 0 78 4 2 2 1 1 0 0 0 0 1 0 0 0 0 1 0 0 36 24 20 4 0 4 0 3 0 0 0 0 0 0 1 0 0 0 51 51 46 5 3 2 1 1 1 0 1 0 1 0 0 0 0 0 30 21 18 3 0 3 1 0 0 0 2 0 0 0 0 0 0 0 117 114 100 14 6 8 3 2 4 0 1 1 0 0 1 0 2 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 (1) 0 0 0 0 aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,319-320(CDR3)| tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,319-320(CDR3)| agct,8-11(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|tacc,262-265(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtct ggtgagtccttcactaattactac tggagctggatccgccagtccccaggaaagggtctggagtggcttggggag gtccatcatagtggacgcacc gactacaacccgtccctcaagagtcgaatcaccatgtcgttagacacgtccgaaaatcagttctccctgaagttgacttctttgaccgccgcggacacggcagtatattattgt gcgagaaataccaactatggtggcaattccggggactactttgactac ARNTNYGGNSGDYFDY IGHV4-34 IGHD4-23 IGHJ4 ggtgagtccttcactaattactac tggagctggatccgccagtccccaggaaagggtctggagtggcttggggag gtccatcatagtggacgcacc gactacaacccgtccctcaagagtcgaatcaccatgtcgttagacacgtccgaaaatcagttctccctgaagttgacttctttgaccgccgcggacacggcagtatattattgt gcgagaaataccaactatggtggcaattccggggactactttgactac IGG2 26 210 16 10 11 16 5 10 9 0 10 7 JY8QFUQ01AQZ7D 7214 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 867 89.72 192/214 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [0.17.38.11] CTRDIGGRYSYW in-frame - cggattcacctttagtagtttctggatgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacggactaatgagtatgggagtatcacaaactacgcggactccgtggaggaccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgtacaagggacatcggtgggcgctactcctactggggccagggagtcctggtctccgtctcatcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [276] t78>c|c90>t|c108>t|a110>t,Y37>F(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|g247>a,A83>T(- - -)|c257>g,T86>R(- - -)|g261>a|g303>t|g313>a,A105>T(- - -)|a318>g| t78>c| c90>t|c108>t|a110>t,Y37>F(- + -)| c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|g247>a,A83>T(- - -)|c257>g,T86>R(- - -)|g261>a|g303>t| g313>a,A105>T(- - -)|a318>g| 320 222 198 24 8 16 4 7 3 2 0 0 1 0 0 1 3 3 78 1 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 2 0 0 0 1 0 0 0 0 0 51 51 46 5 2 3 1 1 1 0 0 0 0 0 0 0 0 2 30 24 18 6 0 6 1 1 0 1 0 0 0 0 0 0 2 1 117 114 107 7 2 5 1 4 0 0 0 0 0 0 0 1 1 0 8 8 6 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| c ggattcacctttagtagtttctgg atgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt acaagggacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttagtagtttctgg atgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt acaagggacatcggtgggcgctactcctac IGG1 21 213 13 8 9 13 4 8 4 2 8 7 JY8QFUQ01AR4F8 19477 productive Homsap IGHV3-49*03 F, or Homsap IGHV3-49*05 F 1038 96.41 215/223 nt NA Homsap IGHJ4*02 F (see comment) 177 85.42 41/48 nt Homsap IGHD3-3*01 F 1 8 10 17 8.10.17 [1.17.38.11] CTRDNPPLRSLEWLPFDSW in-frame - Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ttctggattcacctttggtgattatagtatgagttggttccgccaggctccagggaaggggctggagtgggtcggtttcattagaagcaaagctgatgatgggacaacagaatacgccgcgtctgtgaaaggcagattcaccatctcaagagatgattccaaaagcatcgcctatctgcaaatgaatagcctgaaaaccggggacacagccgtgtattactgtactagagataaccctccattgcgatctttggagtggttaccctttgactcctggggccagggaaccctagtcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [305] g112>a,A38>S(- + -)|c113>g,A38>S(- + -)|c120>t|a159>c|t181>g,Y61>D(- - -)|g185>a,G62>D(- - -)|c276>t|a290>g,E97>G(- - -)| g112>a,A38>S(- + -)|c113>g,A38>S(- + -)| c120>t|a159>c| t181>g,Y61>D(- - -)|g185>a,G62>D(- - -)| c276>t|a290>g,E97>G(- - -)| 320 231 223 8 3 5 1 2 2 0 1 0 0 0 0 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 0 0 1 0 0 51 51 49 2 2 0 0 0 1 0 1 0 0 0 0 0 0 0 30 30 28 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 117 114 112 2 1 1 1 0 1 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,158-159(FR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,181-182(CDR2)|aa,192-193(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|ta,69-70(FR1)|tt,75-76(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,124-125(FR2)|ta,158-159(FR2)|tt,162-163(FR2)|tt,167-168(CDR2)|tt,180-181(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agct,72-75(FR1)|agct,118-121(FR2)|ggtt,122-125(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggta,156-159(FR2)|ggtt,160-163(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|ggca,220-223(FR3)|agca,253-256(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,72-75(FR1)|tgct,111-114(CDR1)|agct,118-121(FR2)|agct,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| ttct ggattcacctttggtgattatagt atgagttggttccgccaggctccagggaaggggctggagtgggtcggtttc attagaagcaaagctgatgatgggacaaca gaatacgccgcgtctgtgaaaggcagattcaccatctcaagagatgattccaaaagcatcgcctatctgcaaatgaatagcctgaaaaccggggacacagccgtgtattactgt actagagataaccctccattgcgatctttggagtggttaccctttgactcc TRDNPPLRSLEWLPFDS IGHV3-49 IGHD3-3 IGHJ4 ggattcacctttggtgattatagt atgagttggttccgccaggctccagggaaggggctggagtgggtcggtttc attagaagcaaagctgatgatgggacaaca gaatacgccgcgtctgtgaaaggcagattcaccatctcaagagatgattccaaaagcatcgcctatctgcaaatgaatagcctgaaaaccggggacacagccgtgtattactgt actagagataaccctccattgcgatctttggagtggttaccctttgactcc IGA1 8 219 5 3 4 5 1 3 3 0 1 4 JY8QFUQ01AR4XZ 13319 productive (see comment) Homsap IGHV3-48*01 F 923 91.74 200/218 nt 91.28 199/218 nt Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD3-10*01 F 2 8 8 14 8.8.14 [1.17.38.11] CTRQTGNYYYFGVDVW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'T' (1 nucleotides at position 10 to 10 in the user submitted sequence), (cause frameshift); cctctggatTtcctcttcagtagctttaacatgaactgggtccgccacgttccagggaagggtctggagtgggtttcatacattaatagtagaggtactaacatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaggaattcactgtatctgcaaatgaacagcctgagagccgacgacacggccgtatactactgtacgagacaaactgggaattattattacttcggtgtggacgtctggggccaagggactacggtcaccgtctcctctgcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [292] a85>c,T29>L(- - -)|c86>t,T29>L(- - -)|a110>t,Y37>F(- + -)|g113>a,S38>N(- - -)|g132>c,Q44>H(- + -)|c134>t,A45>V(+ - +)|g147>t|g170>a,S57>N(- - -)|t177>a,S59>R(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|c191>a,T64>N(- + -)|a251>g,K84>R(+ + +)|c255>t|g291>c,E97>D(+ - +)|t300>c|g303>a|t306>c|g313>a,A105>T(- - -)| a85>c,T29>L(- - -)|c86>t,T29>L(- - -)|a110>t,Y37>F(- + -)|g113>a,S38>N(- - -)| g132>c,Q44>H(- + -)|c134>t,A45>V(+ - +)|g147>t| g170>a,S57>N(- - -)|t177>a,S59>R(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|c191>a,T64>N(- + -)| a251>g,K84>R(+ + +)|c255>t|g291>c,E97>D(+ - +)|t300>c|g303>a|t306>c| g313>a,A105>T(- - -)|g319>c| 318 (320) 224 (226) 205 (206) 19 (20) 5 (6) 14 2 4 3 2 1 1 1 1 3 (4) 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 1 0 1 0 1 0 0 0 0 0 51 51 48 3 1 2 0 0 1 0 0 0 0 0 1 0 1 0 30 24 19 5 0 5 1 1 0 0 0 1 0 1 1 0 0 0 117 114 108 6 4 2 1 1 1 2 0 0 0 0 1 0 0 0 6 (8) 6 5 (6) 1 (2) 0 (1) 1 0 1 0 0 0 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcctcttcagtagctttaac atgaactgggtccgccacgttccagggaagggtctggagtgggtttcatac attaatagtagaggtactaacata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaggaattcactgtatctgcaaatgaacagcctgagagccgacgacacggccgtatactactgt acgagacaaactgggaattattattacttcggtgtggacgtc TRQTGNYYYFGVDV IGHV3-48 IGHD3-10 IGHJ6 ggattcctcttcagtagctttaac atgaactgggtccgccacgttccagggaagggtctggagtgggtttcatac attaatagtagaggtactaacata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaggaattcactgtatctgcaaatgaacagcctgagagccgacgacacggccgtatactactgt acgagacaaactgggaattattattacttcggtgtggacgtc IGA1 18 213 10 8 6 11 4 7 5 0 4 9 JY8QFUQ01AR7NH 11057 productive Homsap IGHV4-61*02 F 888 89.55 197/220 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-19*01 F 1 10 7 13 10.7.13 [1.17.38.11] CARQGDSSGPWNDYW in-frame + ctccggtggctccatcagtactggttattactactggagctggatccggcagtccgccgggaagggactggaatggattgggcgcatgtctgccagaggggacagcaactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgtgcgagacaaggggatagtagtggaccctggaatgactactggggccagggaatcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [291] t78>c|c93>t|g101>c,S34>T(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c133>t,P45>S(+ - -)|g153>a|t165>c|c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>g,T65>S(+ - +)|a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| t78>c| c93>t|g101>c,S34>T(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)| c133>t,P45>S(+ - -)|g153>a|t165>c| c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>g,T65>S(+ - +)| a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g319>c| 318 (320) 226 (228) 203 (204) 23 (24) 5 (6) 18 3 4 3 2 3 0 2 1 2 (3) 3 0 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 30 26 4 1 3 0 1 1 0 0 0 1 0 1 0 0 0 51 51 48 3 2 1 0 1 1 1 0 0 0 0 0 0 0 0 30 21 14 7 0 7 2 1 0 0 1 0 0 1 0 2 0 0 117 114 106 8 1 7 1 1 1 0 2 0 1 0 1 1 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctcc ggtggctccatcagtactggttattactac tggagctggatccggcagtccgccgggaagggactggaatggattgggcgc atgtctgccagaggggacagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac ARQGDSSGPWNDY IGHV4-61 IGHD6-19 IGHJ4 ggtggctccatcagtactggttattactac tggagctggatccggcagtccgccgggaagggactggaatggattgggcgc atgtctgccagaggggacagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac IGG2 22 216 11 11 7 12 4 10 3 1 8 10 JY8QFUQ01AR8K0 10171 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 901 90.41 198/219 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD6-13*01 F 2 8 8 14 8.8.14 [2.17.38.11] CARRTITAAGRGDDYW in-frame - gcctctgaattcacctttagcagttttgccatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaagcattggtactagtgttgttaacacatggtacgcagactcagtgaagggccggttcgccatttccagagacaattccaagagcacgctgtatttgcaaatgaatagcctgagagtcgaggacacggccgtatattactgtgcgaggaggacaataacagcagctggccgtggggatgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [292] g80>a,G27>E(- - -)|c108>t|a110>t,Y37>F(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t165>c,A55>S(- + -)|a169>g,S57>G(+ + -)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|g185>t,G62>V(- - -)|g188>t,G63>V(- - -)|g191>a,S64>N(- - -)|a197>g,Y66>W(- + -)|c198>g,Y66>W(- + -)|c210>a|a229>g,T77>A(- - -)|c234>t|a254>g,N85>S(- - -)|c265>t|c276>t|c287>t,A96>V(+ - +)| g80>a,G27>E(- - -)|c108>t|a110>t,Y37>F(- + -)| g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t165>c,A55>S(- + -)| a169>g,S57>G(+ + -)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|g185>t,G62>V(- - -)|g188>t,G63>V(- - -)|g191>a,S64>N(- - -)| a197>g,Y66>W(- + -)|c198>g,Y66>W(- + -)|c210>a|a229>g,T77>A(- - -)|c234>t|a254>g,N85>S(- - -)|c265>t|c276>t|c287>t,A96>V(+ - +)| a317>g,K106>R(+ + +)|a318>g,K106>R(+ + +)|g319>a|a320>g| 316 (320) 223 (227) 202 21 (25) 5 (7) 16 (18) 4 (7) 4 (5) 5 1 0 1 1 0 1 2 2 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 0 0 0 1 0 0 0 0 0 51 51 48 3 0 3 0 1 0 1 0 0 0 0 0 1 0 0 30 24 18 6 0 6 1 2 0 0 0 0 0 0 1 0 2 0 117 114 105 9 4 5 3 0 4 0 0 1 0 0 0 1 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (3) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct gaattcacctttagcagttttgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaagc attggtactagtgttgttaacaca tggtacgcagactcagtgaagggccggttcgccatttccagagacaattccaagagcacgctgtatttgcaaatgaatagcctgagagtcgaggacacggccgtatattactgt gcgaggaggacaataacagcagctggccgtggggatgactac ARRTITAAGRGDDY IGHV3-23 IGHD6-13 IGHJ4 gaattcacctttagcagttttgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaagc attggtactagtgttgttaacaca tggtacgcagactcagtgaagggccggttcgccatttccagagacaattccaagagcacgctgtatttgcaaatgaatagcctgagagtcgaggacacggccgtatattactgt gcgaggaggacaataacagcagctggccgtggggatgactac IGA2 21 213 14 7 9 15 5 6 4 1 8 8 JY8QFUQ01AR8UJ 10677 productive (see comment) Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F or Homsap IGHV3-15*04 F 1002 94.62 211/223 nt 94.17 210/223 nt Homsap IGHJ1*01 F (see comment) 161 78.85 41/52 nt Homsap IGHD1-26*01 F 3 8 10 9 8.10.9 [1.17.37.11] CTTGGGTNYYW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR3-IMGT, from codon 84 of V-REGION: 1 nucleotides (from position 162 in the user submitted sequence), (cause frameshift) cctctggattcactttcagtgacgcctggatgagctgggtccgccaggctccagggaaagggctggagtgggttggccgtattccaagcaaagctgatggtgggacaacagactacgctgcgcccgttaaaggcagattcaccatctcaagagaggattcaaaaatatgctgtatctgcaattgaacagcctgaaaaccgaggacacagccgtgtatttctgtaccacagggggtggaactaactactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [282] a106>g,N36>D(+ + -)|g144>a|a169>c,K57>P(- - -)|a170>c,K57>P(- - -)|a178>g,T60>A(- - -)|a207>g|g213>t|t243>g,D81>E(+ - +)|c255>t|c257>t,T86>M(- - -)|a271>t,M91>L(+ + -)|a308>t,Y103>F(- + -)| a106>g,N36>D(+ + -)| g144>a| a169>c,K57>P(- - -)|a170>c,K57>P(- - -)|a178>g,T60>A(- - -)| a207>g|g213>t|t243>g,D81>E(+ - +)|c255>t|c257>t,T86>M(- - -)|a271>t,M91>L(+ + -)|a308>t,Y103>F(- + -)| a320>g| 319 (320) 230 (231) 218 12 (13) 4 (5) 8 3 (4) 1 2 0 2 0 2 0 0 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 30 27 3 0 3 1 0 0 0 2 0 0 0 0 0 0 0 117 113 106 7 3 4 1 0 2 0 0 0 2 0 0 0 1 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| cctct ggattcactttcagtgacgcctgg atgagctgggtccgccaggctccagggaaagggctggagtgggttggccgt attccaagcaaagctgatggtgggacaaca gactacgctgcgcccgttaaaggcagattcaccatctcaagagaggattcaaaaatatgctgtatctgcaattgaacagcctgaaaaccgaggacacagccgtgtatttctgt accacagggggtggaactaactactac TTGGGTNYY IGHV3-15 IGHD1-26 IGHJ1 ggattcactttcagtgacgcctgg atgagctgggtccgccaggctccagggaaagggctggagtgggttggccgt attccaagcaaagctgatggtgggacaaca gactacgctgcgcccgttaaaggcagattcaccatctcaagagaggattcaaaaatatgctgtatctgcaattgaacagcctgaaaaccgaggacacagccgtgtatttctgt accacagggggtggaactaactactac IGG1 12 218 6 6 3 4 3 8 4 0 4 4 JY8QFUQ01AR91V 17889 productive (see comment) Homsap IGHV4-61*02 F (see comment) 779 84.4 184/218 nt 83.03 181/218 nt Homsap IGHJ5*02 F 201 88.24 45/51 nt Homsap IGHD4-17*01 F 2 9 7 13 9.7.13 [1.17.38.11] CARAKDHDDYLVDPW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (84,40% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR1-IMGT at codon 37 of V-REGION: 'CTT' (3 nucleotides at position 28 to 30 in the user submitted sequence), (do not cause frameshift);in FR3-IMGT at codon 68 of V-REGION: 'T' (1 nucleotides at position 116 to 116 in the user submitted sequence), (cause frameshift); in CDR1-IMGT, from codon 36 of V-REGION: 3 nucleotides (from position 27 in the user submitted sequence), (do not cause frameshift) tctctggtgcctccatcactagtggtaCTTactactggagttggttccggcagcccgccgggaagacactggaatgggttgggcgtatctatacaactgggagcacctattacaaTcccctctctcaaaagccgagtcaccgtttcagtcgacgagtcccagaatcagctcttcctggacctgacttctgtgaccgccgcggacacggccgtctacttctgtgcgcgagctaaggaccacgatgactacctggtcgacccctggggccaggggatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [292] g83>c,G28>A(- + -)|g92>c,S31>T(+ - +)|c93>t,S31>T(+ - +)|t109>a,Y37>N(- - -)|c120>t|a124>t,I42>F(+ - -)|g145>a,G49>T(+ - -)|g146>c,G49>T(+ - -)|g153>a|a157>g,I53>V(+ - +)|c174>a|g176>c,S59>T(+ - +)|a196>t,N66>Y(- - -)|c198>t,N66>Y(- - -)|c210>t|g216>a|t222>c|a232>g,I78>V(+ - +)|a234>t,I78>V(+ - +)|a240>c|a244>g,T82>E(- - -)|c245>a,T82>E(- - -)|a250>c,K84>Q(+ - -)|c255>t|t259>c,F87>L(+ - -)|c263>t,S88>F(- - -)|a268>g,K90>D(+ - -)|g270>c,K90>D(+ - -)|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|a291>g|g303>c|t306>c|a308>t,Y103>F(- + -)|a316>c| g83>c,G28>A(- + -)|g92>c,S31>T(+ - +)|c93>t,S31>T(+ - +)|t109>a,Y37>N(- - -)| c120>t|a124>t,I42>F(+ - -)|g145>a,G49>T(+ - -)|g146>c,G49>T(+ - -)|g153>a|a157>g,I53>V(+ - +)| c174>a|g176>c,S59>T(+ - +)| a196>t,N66>Y(- - -)|c198>t,N66>Y(- - -)|c210>t|g216>a|t222>c|a232>g,I78>V(+ - +)|a234>t,I78>V(+ - +)|a240>c|a244>g,T82>E(- - -)|c245>a,T82>E(- - -)|a250>c,K84>Q(+ - -)|c255>t|t259>c,F87>L(+ - -)|c263>t,S88>F(- - -)|a268>g,K90>D(+ - -)|g270>c,K90>D(+ - -)|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|a291>g|g303>c|t306>c|a308>t,Y103>F(- + -)| a316>c|a320>c| 319 (320) 225 (226) 190 35 (36) 12 (13) 23 5 3 7 3 3 (4) 2 4 1 7 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 27 23 4 0 4 0 0 1 0 0 0 0 1 2 0 0 0 51 51 45 6 2 4 1 2 1 0 0 0 1 0 1 0 0 0 30 21 19 2 1 1 0 0 0 0 0 1 0 0 1 0 0 0 117 114 92 22 8 14 4 1 5 3 2 1 3 0 3 0 0 0 7 (8) 7 6 1 (2) 1 (2) 0 0 0 0 0 1 (2) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgcctccatcactagtggtaactac tggagttggttccggcagcccgccgggaagacactggaatgggttgggcgt atctatacaactgggagcacc tattacaacccctctctcaaaagccgagtcaccgtttcagtcgacgagtcccagaatcagctcttcctggacctgacttctgtgaccgccgcggacacggccgtctacttctgt gcgcgagctaaggaccacgatgactacctggtcgacccc ARAKDHDDYLVDP IGHV4-61 IGHD4-17 IGHJ5 ggtgcctccatcactagtggtaactac tggagttggttccggcagcccgccgggaagacactggaatgggttgggcgt atctatacaactgggagcacc tattacaacccctctctcaaaagccgagtcaccgtttcagtcgacgagtcccagaatcagctcttcctggacctgacttctgtgaccgccgcggacacggccgtctacttctgt gcgcgagctaaggaccacgatgactacctggtcgacccc IGA1 34 213 18 16 10 19 8 15 10 1 18 5 JY8QFUQ01ARJHI 18835 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 909 91.24 198/217 nt NA Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD3-3*01 F 2 8 8 21 8.8.21 [1.17.38.11] CVRVGAPYYDFWSGYQTNWFDPW in-frame - gtctggattcagcatcagtagttatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattagtgcaagtactacttccatacattatgcagactcagtgaagggccgattcaccatctccagagacgacgccaagagttccctgtatttgcaaatgaacagcctgagagccgaggacacggctgtatattactgtgtaagagtgggggcgccatattacgatttctggagtggctatcagactaactggttcgacccctggggccagggaactctggtcaccgttacctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccccag NA IGA1 100 100 [311] c75>g|c86>g,T29>S(+ - +)|t88>a,F30>I(+ - -)|c108>t|a172>g,S58>A(- + -)|g173>c,S58>A(- + -)|t174>a,S58>A(- + -)|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|a191>c,Y64>S(+ - -)|t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|a244>g,N82>D(+ + -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a258>c|c265>t|g303>a|c314>t,A105>V(+ - +)|g315>a,A105>V(+ - +)| c75>g| c86>g,T29>S(+ - +)|t88>a,F30>I(+ - -)|c108>t| a172>g,S58>A(- + -)|g173>c,S58>A(- + -)|t174>a,S58>A(- + -)|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|a191>c,Y64>S(+ - -)| t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|a244>g,N82>D(+ + -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a258>c|c265>t|g303>a| c314>t,A105>V(+ - +)|g315>a,A105>V(+ - +)|a320>t| 319 (320) 224 (225) 203 21 (22) 6 (7) 15 3 2 6 1 2 0 0 (1) 2 3 2 0 0 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 21 3 1 2 0 0 1 0 0 0 0 1 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 18 6 0 6 1 0 0 0 1 0 0 1 3 0 0 0 117 114 105 9 4 5 2 1 4 1 1 0 0 0 0 0 0 0 7 (8) 7 5 2 (3) 0 (1) 2 0 1 1 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcagcatcagtagttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtgcaagtactacttccata cattatgcagactcagtgaagggccgattcaccatctccagagacgacgccaagagttccctgtatttgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gtaagagtgggggcgccatattacgatttctggagtggctatcagactaactggttcgacccc VRVGAPYYDFWSGYQTNWFDP IGHV3-21 IGHD3-3 IGHJ5 ggattcagcatcagtagttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtgcaagtactacttccata cattatgcagactcagtgaagggccgattcaccatctccagagacgacgccaagagttccctgtatttgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gtaagagtgggggcgccatattacgatttctggagtggctatcagactaactggttcgacccc IGA1 18 213 10 8 6 10 4 8 4 1 5 8 JY8QFUQ01ARLMX 7401 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 935 93.02 200/215 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD3-22*01 F 2 8 7 17 8.7.17 [1.17.38.11] CAKNLRLGHSYFYNALDVW in-frame + tctctggtggcctcatcagtaattactattggacctggatccggcagcccccaggaaagggactggagtggattgggaacatctattacagtgggagcgccacctacaacccctccctcaagagtcgagtcaccatatcaatagactcgtccaagaaccagttctccctgaggctgaccgctgtgaccgctgcggacacggccgtgtattactgtgcgaaaaacttacgattgggtcacagttacttctacaacgctttggacgtatggggccaagggaccacggtcaccgtcgcctcaacatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] t85>c,S29>L(- - -)|c86>t,S29>L(- - -)|g107>a,S36>N(- - -)|c114>t|g119>c,S40>T(+ - +)|g141>a|t163>a,Y55>N(- - -)|t165>c,Y55>N(- - -)|a193>g,T65>A(- - -)|a197>c,N66>T(- + -)|g238>a,V80>I(+ - +)|a244>t,T82>S(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|t277>g,S93>A(- + -)| t85>c,S29>L(- - -)|c86>t,S29>L(- - -)|g107>a,S36>N(- - -)|c114>t| g119>c,S40>T(+ - +)|g141>a|t163>a,Y55>N(- - -)|t165>c,Y55>N(- - -)| a193>g,T65>A(- - -)| a197>c,N66>T(- + -)|g238>a,V80>I(+ - +)|a244>t,T82>S(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|t277>g,S93>A(- + -)| g317>a,R106>K(+ + +)|g319>a| 316 (320) 219 (223) 204 (206) 15 (17) 2 (3) 13 (14) 2 3 (5) 2 2 1 0 1 1 2 0 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 2 1 0 0 0 0 0 0 0 0 51 51 47 4 1 3 0 1 0 1 0 0 0 1 1 0 0 0 30 21 20 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 0 6 1 1 0 0 1 0 1 0 1 0 0 1 4 (8) 4 4 (6) 0 (2) 0 (1) 0 (1) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggcctcatcagtaattactat tggacctggatccggcagcccccaggaaagggactggagtggattgggaac atctattacagtgggagcgcc acctacaacccctccctcaagagtcgagtcaccatatcaatagactcgtccaagaaccagttctccctgaggctgaccgctgtgaccgctgcggacacggccgtgtattactgt gcgaaaaacttacgattgggtcacagttacttctacaacgctttggacgta AKNLRLGHSYFYNALDV IGHV4-59 IGHD3-22 IGHJ6 ggtggcctcatcagtaattactat tggacctggatccggcagcccccaggaaagggactggagtggattgggaac atctattacagtgggagcgcc acctacaacccctccctcaagagtcgagtcaccatatcaatagactcgtccaagaaccagttctccctgaggctgaccgctgtgaccgctgcggacacggccgtgtattactgt gcgaaaaacttacgattgggtcacagttacttctacaacgctttggacgta IGA1 15 210 9 6 5 7 4 8 1 1 9 4 JY8QFUQ01AROW2 17780 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 914 91.28 199/218 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD6-19*01 F 2 8 8 14 8.8.14 [1.17.38.11] CAKEIAVSGEGALDFW in-frame + cctctggattcaccttcagtaactatagcatgagctgggtccgccaggctccagggaaggggccggagtgggtctcagctattactggtcttgatggtagaacattctacgcagactccgtgaagggccgtttctccatctccagagacaattccaagaacacgttgtatttgggaatgaacagcctgagagccgaggacacggccgtatattattgtgcgaaagagatagcagtgagtggtgagggggcgttggacttctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttctttcccca NA IGA1 100 100 [291] t90>c|c105>t|g107>a,S36>N(- - -)|g112>a,A38>S(- + -)|c113>g,A38>S(- + -)|t149>c,L50>P(- - -)|g170>c,S57>T(+ - +)|a175>c,S59>L(- - -)|g176>t,S59>L(- - -)|g185>a,G62>D(- - -)|c192>a,S64>R(- - -)|a197>t,Y66>F(- + -)|g225>t|a229>t,T77>S(+ - +)|c259>t|c265>t|c268>g,Q90>G(- - -)|a269>g,Q90>G(- - -)|c309>t| t90>c|c105>t|g107>a,S36>N(- - -)|g112>a,A38>S(- + -)|c113>g,A38>S(- + -)| t149>c,L50>P(- - -)| g170>c,S57>T(+ - +)|a175>c,S59>L(- - -)|g176>t,S59>L(- - -)|g185>a,G62>D(- - -)|c192>a,S64>R(- - -)| a197>t,Y66>F(- + -)|g225>t|a229>t,T77>S(+ - +)|c259>t|c265>t|c268>g,Q90>G(- - -)|a269>g,Q90>G(- - -)|c309>t| 320 226 207 19 6 13 1 3 4 2 1 1 2 0 1 2 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 2 1 1 0 0 0 0 0 1 0 0 51 51 50 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 30 24 19 5 0 5 0 1 0 0 1 1 0 0 1 0 1 0 117 114 106 8 4 4 1 0 3 0 0 0 2 0 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtaactatagc atgagctgggtccgccaggctccagggaaggggccggagtgggtctcagct attactggtcttgatggtagaaca ttctacgcagactccgtgaagggccgtttctccatctccagagacaattccaagaacacgttgtatttgggaatgaacagcctgagagccgaggacacggccgtatattattgt gcgaaagagatagcagtgagtggtgagggggcgttggacttc AKEIAVSGEGALDF IGHV3-23 IGHD6-19 IGHJ4 ggattcaccttcagtaactatagc atgagctgggtccgccaggctccagggaaggggccggagtgggtctcagct attactggtcttgatggtagaaca ttctacgcagactccgtgaagggccgtttctccatctccagagacaattccaagaacacgttgtatttgggaatgaacagcctgagagccgaggacacggccgtatattattgt gcgaaagagatagcagtgagtggtgagggggcgttggacttc IGA1 19 213 10 9 7 13 3 6 4 2 5 8 JY8QFUQ01ARPRY 12637 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 981 94.93 206/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD5-12*01 F 1 8 8 14 8.8.14 [1.17.38.11] CAKDLFQVSTTYFDYW in-frame + ctctggattcaccttcagtgactatcacatgtactgggtccgccaggctccaggcaaggggctggagtgggtggcagttatatcatatgatggaagtaataaatactatgtagactccgtgaagggccgattcaccatctccagagacaattccaagaatgcgctgtttctgcagatgaacagcctgagagctgacgacacggctgtgtattactgtgcgaaagacctctttcaggtgtctacgacttactttgactactggggccggggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggctgtcgtggaactc NA IGG2 100 100 [291] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g112>c,G38>H(+ - -)|g113>a,G38>H(+ - -)|c118>t,H40>Y(+ - -)|c203>t,A68>V(+ - +)|c255>t|a256>g,T86>A(- - -)|a263>t,Y88>F(- + -)|a270>g|g291>c,E97>D(+ - +)|g317>a,R106>K(+ + +)| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g112>c,G38>H(+ - -)|g113>a,G38>H(+ - -)| c118>t,H40>Y(+ - -)| c203>t,A68>V(+ - +)|c255>t|a256>g,T86>A(- - -)|a263>t,Y88>F(- + -)|a270>g|g291>c,E97>D(+ - +)| g317>a,R106>K(+ + +)| 320 225 213 12 2 10 3 3 3 0 0 0 1 0 2 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 2 0 0 0 0 0 0 1 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 2 4 2 0 2 0 0 0 1 0 1 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgactatcac atgtactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcatatgatggaagtaataaa tactatgtagactccgtgaagggccgattcaccatctccagagacaattccaagaatgcgctgtttctgcagatgaacagcctgagagctgacgacacggctgtgtattactgt gcgaaagacctctttcaggtgtctacgacttactttgactac AKDLFQVSTTYFDY IGHV3-30 IGHD5-12 IGHJ4 ggattcaccttcagtgactatcac atgtactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcatatgatggaagtaataaa tactatgtagactccgtgaagggccgattcaccatctccagagacaattccaagaatgcgctgtttctgcagatgaacagcctgagagctgacgacacggctgtgtattactgt gcgaaagacctctttcaggtgtctacgacttactttgactac IGG2 11 213 8 3 5 7 3 4 2 0 5 4 JY8QFUQ01ARWZ0 21818 productive Homsap IGHV3-7*01 F 959 93.58 204/218 nt NA Homsap IGHJ5*02 F (see comment) 138 74.51 38/51 nt NA 8 8 9 8.8.9 [1.17.38.11] CTTTLVWFGEL (see Functionality comment) in-frame - IMGT/JunctionAnalysis gives no results for this JUNCTION Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) cctctggattcacgtttagagactattggatgagttgggtccgccaggctcctgggagggggctggagtgggtggccaacataaagcaagatgcaagtgaggaatactatgtggactctgtgaagggccggttcaccatctccagagacaacgccaagagctcactgcatttgcaaatgaacagcctgagagccgaggacacggctatgtattactgtacgaccacattagtatggttcggggagttaggccagggaacccaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [276, 275] c87>g|t105>a,S35>R(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c120>t|a138>t|a143>g,K48>R(+ + +)|g185>c,G62>A(- + -)|a193>g,K65>E(+ - -)|a225>g|a254>g,N85>S(- - -)|t262>c,Y88>H(+ - -)|c265>t|g301>a,V101>M(+ - -)|g313>a,A105>T(- - -)| c87>g|t105>a,S35>R(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| c120>t|a138>t|a143>g,K48>R(+ + +)| g185>c,G62>A(- + -)|a193>g,K65>E(+ - -)| a225>g|a254>g,N85>S(- - -)|t262>c,Y88>H(+ - -)|c265>t|g301>a,V101>M(+ - -)| g313>a,A105>T(- - -)|g317>c,R106>T(- - -)|a318>c,R106>T(- - -)|g319>a|a320>c| 316 (320) 222 (226) 207 15 (19) 5 (7) 10 (12) 5 3 (4) 2 1 0 (2) 0 1 1 1 (2) 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 0 0 0 0 0 1 0 1 0 0 51 51 48 3 2 1 1 0 1 0 0 0 1 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 117 114 109 5 2 3 2 1 1 1 0 0 0 0 0 0 0 0 4 (8) 4 3 1 (5) 0 (2) 1 (3) 0 1 (2) 0 0 0 (2) 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacgtttagagactattgg atgagttgggtccgccaggctcctgggagggggctggagtgggtggccaac ataaagcaagatgcaagtgaggaa tactatgtggactctgtgaagggccggttcaccatctccagagacaacgccaagagctcactgcatttgcaaatgaacagcctgagagccgaggacacggctatgtattactgt acgaccacattagtatggttcggggag TTTLVWFGE IGHV3-7 IGHJ5 ggattcacgtttagagactattgg atgagttgggtccgccaggctcctgggagggggctggagtgggtggccaac ataaagcaagatgcaagtgaggaa tactatgtggactctgtgaagggccggttcaccatctccagagacaacgccaagagctcactgcatttgcaaatgaacagcctgagagccgaggacacggctatgtattactgt acgaccacattagtatggttcggggag IGA2 14 213 10 4 4 6 6 8 4 1 4 5 JY8QFUQ01ARYWV 8743 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 850 88.43 191/216 nt NA Homsap IGHJ5*02 F 228 94.12 48/51 nt Homsap IGHD6-6*01 F 2 8 8 14 8.8.14 [1.17.38.11] CAKCKAPGKVDWFDPW in-frame + tctggattcacctttagcggctatgccatggcttgggtccgccaggctccagggaaggggctggagtgggtctcaactagtactactgatggagctggcccatactacgcagactccgtgaggggccggttcaccgtcttcagagacaattccaagaacactctgtatctacaaatggacaccctgagagccgacgacacggccatgtattactgtgcgaaatgtaaagcgcctggtaaggtggactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccggaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctgtccagggcttctt NA IGA1 100 95 [290] a106>g,S36>G(+ + -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c120>t,S40>A(- + -)|g163>a,A55>T(- - -)|t167>g,I56>S(- - -)|g170>c,S57>T(+ - +)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|t186>a|g188>c,G63>A(- + -)|a190>g,S64>G(+ + -)|a193>c,T65>P(+ + -)|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|c236>t,S79>F(- - -)|g258>t|g267>a|a274>g,N92>D(+ + -)|g278>c,S93>T(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>M(+ - -)|a303>g,V101>M(+ - -)| a106>g,S36>G(+ + -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c120>t,S40>A(- + -)|g163>a,A55>T(- - -)| t167>g,I56>S(- - -)|g170>c,S57>T(+ - +)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|t186>a|g188>c,G63>A(- + -)|a190>g,S64>G(+ + -)|a193>c,T65>P(+ + -)| a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|c236>t,S79>F(- - -)|g258>t|g267>a|a274>g,N92>D(+ + -)|g278>c,S93>T(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>M(+ - -)|a303>g,V101>M(+ - -)| g319>t|a320>g| 318 (320) 222 (224) 197 25 (27) 3 (5) 22 8 (9) 5 2 0 1 0 0 1 6 0 1 (2) 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 51 47 4 0 4 1 1 1 0 0 0 0 0 1 0 0 0 30 24 14 10 1 9 2 2 0 0 1 0 0 1 3 0 0 1 117 114 104 10 2 8 4 2 1 0 0 0 0 0 2 0 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcacctttagcggctatgcc atggcttgggtccgccaggctccagggaaggggctggagtgggtctcaact agtactactgatggagctggccca tactacgcagactccgtgaggggccggttcaccgtcttcagagacaattccaagaacactctgtatctacaaatggacaccctgagagccgacgacacggccatgtattactgt gcgaaatgtaaagcgcctggtaaggtggactggttcgacccc AKCKAPGKVDWFDP IGHV3-23 IGHD6-6 IGHJ5 ggattcacctttagcggctatgcc atggcttgggtccgccaggctccagggaaggggctggagtgggtctcaact agtactactgatggagctggccca tactacgcagactccgtgaggggccggttcaccgtcttcagagacaattccaagaacactctgtatctacaaatggacaccctgagagccgacgacacggccatgtattactgt gcgaaatgtaaagcgcctggtaaggtggactggttcgacccc IGA1 25 213 15 10 7 14 8 11 2 1 12 10 JY8QFUQ01AS13P 5984 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 918 91.71 199/217 nt NA Homsap IGHJ5*02 F 255 100 51/51 nt Homsap IGHD3-22*01 F 1 8 8 16 8.8.16 [1.17.38.11] CAKAGRGLIRPNNWFDPW in-frame + ctctggatttaccttcagtggctatggcatgcactgggtccgccaggctccaggcaagggcctggagtgggtgacagttgtttcatatgatggaagtattaagaattatgcagactccgtgaagggccgattcaccatctccagagacgattccaagaatacgctgtatctgcaaatgagcagcctgggacctgaagacacggctatatattactgtgcgaaagcggggcgtgggttgatacgacccaacaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [296] c84>t|a106>g,S36>G(+ + -)|g147>c|g160>a,A54>T(- - -)|a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|a191>t,N64>I(- - -)|a195>g|t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|a244>g,N82>D(+ + -)|c255>t|a275>g,N92>S(- - -)|a283>g,R95>G(- - -)|g286>c,A96>P(- - -)|g291>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|g317>a,R106>K(+ + +)| c84>t|a106>g,S36>G(+ + -)| g147>c|g160>a,A54>T(- - -)| a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|a191>t,N64>I(- - -)|a195>g| t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|a244>g,N82>D(+ + -)|c255>t|a275>g,N92>S(- - -)|a283>g,R95>G(- - -)|g286>c,A96>P(- - -)|g291>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g317>a,R106>K(+ + +)|a320>c| 319 (320) 224 (225) 205 19 (20) 5 (6) 14 6 5 3 0 0 (1) 0 2 1 2 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 1 0 1 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 30 24 20 4 1 3 2 0 0 0 0 0 2 0 0 0 0 0 117 114 104 10 2 8 3 3 2 0 0 0 0 1 1 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 1 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggatttaccttcagtggctatggc atgcactgggtccgccaggctccaggcaagggcctggagtgggtgacagtt gtttcatatgatggaagtattaag aattatgcagactccgtgaagggccgattcaccatctccagagacgattccaagaatacgctgtatctgcaaatgagcagcctgggacctgaagacacggctatatattactgt gcgaaagcggggcgtgggttgatacgacccaacaactggttcgacccc AKAGRGLIRPNNWFDP IGHV3-30 IGHD3-22 IGHJ5 ggatttaccttcagtggctatggc atgcactgggtccgccaggctccaggcaagggcctggagtgggtgacagtt gtttcatatgatggaagtattaag aattatgcagactccgtgaagggccgattcaccatctccagagacgattccaagaatacgctgtatctgcaaatgagcagcctgggacctgaagacacggctatatattactgt gcgaaagcggggcgtgggttgatacgacccaacaactggttcgacccc IGA1 18 213 13 5 7 9 6 9 3 2 9 4 JY8QFUQ01AS1T0 6999 productive Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 954 93.55 203/217 nt NA Homsap IGHJ3*02 F 196 88 44/50 nt Homsap IGHD2-8*01 F 3 8 8 10 8.8.10 [1.17.38.11] CAREVSDAFEMW in-frame + ctctggattcatctttgatgattttggcatgaattgggtccgccaagctccagggaaggggctggagtgggtcgctggtatcagttggaatggtggcaaagcaggtcacgcagactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgagagccgaggacacggccgtgtatcactgtgcgagagaggtgtctgatgcttttgaaatgtggggtcaagggacaacggtcaatgtctcttcagcatccccgaccagcccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccga NA IGA1 100 95 [277, 278] c86>t,T29>I(- - -)|a110>t,Y37>F(- + -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|t160>g,S54>A(- + -)|t168>c|a170>g,N57>S(- - -)|t189>c|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|a193>g,T65>A(- - -)|t199>c,Y67>H(+ - -)|t201>c,Y67>H(+ - -)|t301>g,L101>V(+ - +)| c86>t,T29>I(- - -)|a110>t,Y37>F(- + -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|t160>g,S54>A(- + -)| t168>c|a170>g,N57>S(- - -)|t189>c|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|a193>g,T65>A(- - -)| t199>c,Y67>H(+ - -)|t201>c,Y67>H(+ - -)|t301>g,L101>V(+ - +)| 320 225 211 14 2 12 2 2 2 4 0 1 1 0 0 0 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 1 0 0 0 1 0 0 0 0 0 51 51 48 3 0 3 0 1 1 0 0 0 0 0 0 0 0 1 30 24 18 6 2 4 2 1 0 2 0 1 0 0 0 0 0 0 117 114 111 3 0 3 0 0 0 2 0 0 0 0 0 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ctct ggattcatctttgatgattttggc atgaattgggtccgccaagctccagggaaggggctggagtgggtcgctggt atcagttggaatggtggcaaagca ggtcacgcagactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgagagccgaggacacggccgtgtatcactgt gcgagagaggtgtctgatgcttttgaaatg AREVSDAFEM IGHV3-20 IGHD2-8 IGHJ3 ggattcatctttgatgattttggc atgaattgggtccgccaagctccagggaaggggctggagtgggtcgctggt atcagttggaatggtggcaaagca ggtcacgcagactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgagagccgaggacacggccgtgtatcactgt gcgagagaggtgtctgatgcttttgaaatg IGA1 14 213 10 4 4 5 6 9 0 2 6 6 JY8QFUQ01AS22B 16488 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 822 87.38 187/214 nt NA Homsap IGHJ6*02 F (see comment) 175 75.81 47/62 nt Homsap IGHD3-10*01 F 2 8 7 14 8.7.14 [1.17.38.11] CAREKGSGPPTGMDVW in-frame + Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*03 (shorter alignment but highest percentage of identity) ctctgggttgaccgtcagtgccgaccacatgtactgggtccgccaggctccagggaaggggctggagtgggtctcagttctttatggcggtggcaccttggactacgcagactccgtgaagggccgattcaccatctccagagacaattcgaggaacactgtgtatcttcagatggagagactgagccccgaggacacggccgtctactactgtgcgagagaaaaaggttcgggcccccccacaggcatggacgtctggggcccagggaccatggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttcccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [288] c84>g,F28>L(+ - -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a109>g,N37>D(+ + -)|t112>c,Y38>H(+ - -)|a118>t,S40>Y(+ - -)|g119>a,S40>Y(+ - -)|a166>c,I56>L(+ + +)|a172>g,S58>G(+ + -)|t189>c|g191>c,S64>T(+ - +)|a193>t,T65>L(- - -)|c194>t,T65>L(- - -)|a195>g,T65>L(- - -)|t196>g,Y66>D(- - -)|c249>g|a251>g,K84>R(+ + +)|g258>t|c259>g,L87>V(+ - +)|a270>g|a274>g,N92>E(+ - -)|c276>g,N92>E(+ - -)|c279>a,S93>R(- - -)|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|g303>c|t306>c| c84>g,F28>L(+ - -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a109>g,N37>D(+ + -)|t112>c,Y38>H(+ - -)| a118>t,S40>Y(+ - -)|g119>a,S40>Y(+ - -)| a166>c,I56>L(+ + +)|a172>g,S58>G(+ + -)|t189>c|g191>c,S64>T(+ - +)|a193>t,T65>L(- - -)|c194>t,T65>L(- - -)|a195>g,T65>L(- - -)| t196>g,Y66>D(- - -)|c249>g|a251>g,K84>R(+ + +)|g258>t|c259>g,L87>V(+ - +)|a270>g|a274>g,N92>E(+ - -)|c276>g,N92>E(+ - -)|c279>a,S93>R(- - -)|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|g303>c|t306>c| 320 222 195 27 6 21 7 1 1 3 2 1 2 0 4 4 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 2 0 0 1 0 0 0 0 1 1 0 0 51 51 49 2 0 2 0 1 0 0 0 0 1 0 0 0 0 0 30 21 14 7 1 6 2 0 1 1 1 0 1 0 1 0 0 0 117 114 101 13 5 8 3 0 0 1 1 1 0 0 2 3 1 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttgaccgtcagtgccgaccac atgtactgggtccgccaggctccagggaaggggctggagtgggtctcagtt ctttatggcggtggcaccttg gactacgcagactccgtgaagggccgattcaccatctccagagacaattcgaggaacactgtgtatcttcagatggagagactgagccccgaggacacggccgtctactactgt gcgagagaaaaaggttcgggcccccccacaggcatggacgtc AREKGSGPPTGMDV IGHV3-53 IGHD3-10 IGHJ6 gggttgaccgtcagtgccgaccac atgtactgggtccgccaggctccagggaaggggctggagtgggtctcagtt ctttatggcggtggcaccttg gactacgcagactccgtgaagggccgattcaccatctccagagacaattcgaggaacactgtgtatcttcagatggagagactgagccccgaggacacggccgtctactactgt gcgagagaaaaaggttcgggcccccccacaggcatggacgtc IGG1 27 210 12 15 2 12 10 15 5 1 10 11 JY8QFUQ01AS24X 3064 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 1008 96.31 209/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-22*01 F 2 8 8 15 8.8.15 [1.17.38.11] CILLYYDSRGVFSTDYW in-frame + ctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggcgtgggtctcacgtattaaaagtgatggcagtggcacaaactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagagcacgctgtttctgcaaatgaacagtctgagagccgaggacacggctgtatattactgtatactgctttactatgatagtagaggtgtcttttcaaccgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 89 [293, 292] t152>c,V51>A(+ - +)|t171>a,N57>K(+ - -)|g186>c|a190>g,S64>G(+ + -)|g197>a,S66>N(- - -)|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|g303>a| t152>c,V51>A(+ - +)| t171>a,N57>K(+ - -)|g186>c|a190>g,S64>G(+ + -)| g197>a,S66>N(- - -)|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|g303>a| g313>a,A105>I(+ - +)|c314>t,A105>I(+ - +)|a316>c,R106>L(- + -)|g317>t,R106>L(- + -)|a318>g,R106>L(- + -)|g319>c|a320>t| 312 (320) 217 (225) 209 (210) 8 (15) 2 (4) 6 (11) 2 (3) 2 (3) 0 (1) 1 0 (1) 0 1 (2) 1 1 (2) 0 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 30 24 21 3 1 2 1 0 0 0 0 0 0 1 1 0 0 0 117 114 110 4 1 3 1 2 0 0 0 0 1 0 0 0 0 0 0 (8) 0 0 (1) 0 (7) 0 (2) 0 (5) 0 (1) 0 (1) 0 (1) 0 0 (1) 0 0 (1) 0 0 (1) 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggcgtgggtctcacgt attaaaagtgatggcagtggcaca aactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagagcacgctgtttctgcaaatgaacagtctgagagccgaggacacggctgtatattactgt atactgctttactatgatagtagaggtgtcttttcaaccgactac ILLYYDSRGVFSTDY IGHV3-74 IGHD3-22 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggcgtgggtctcacgt attaaaagtgatggcagtggcaca aactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagagcacgctgtttctgcaaatgaacagtctgagagccgaggacacggctgtatattactgt atactgctttactatgatagtagaggtgtcttttcaaccgactac IGA2 8 213 5 3 2 3 3 5 1 1 4 2 JY8QFUQ01AS28D 8659 productive Homsap IGHV3-23*03 F (see comment) 522 71.43 155/217 nt NA Homsap IGHJ2*01 F 166 79.25 42/53 nt Homsap IGHD3-3*01 F 2 8 8 12 8.8.12 [1.17.38.11] CARVRNSNWYFDHW in-frame + Low V-REGION identity (71,43% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctgaagacatgtttaagagtttcggattgacgtgggtccgtcaggctccagggaaggggctggagtgggtcgcaggaataaacaattacaatggtgcaactttctatgctggccccgtaaagggccgcttcaccgtctctcgagataaagaaaagaccattttttatctacaaatggacaacgtgagggtcgacgacacgggcgtttatttctgtgcgagagtcaggaatagtaattggtacttcgaccactggggccgcggcgcactagtcacagtctccgcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] g80>a,G27>E(- - -)|t82>g,F28>D(- - -)|t83>a,F28>D(- - -)|c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|g104>a,S35>K(- - -)|c105>g,S35>K(- - -)|c108>t|a110>t,Y37>F(- + -)|t111>c,Y37>F(- + -)|c113>g,A38>G(- + -)|c114>a,A38>G(- + -)|a115>t,M39>L(+ + -)|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c129>t|t160>g,S54>A(- + -)|t164>g,V55>G(- - -)|t165>a,V55>G(- - -)|t168>a|t169>a,Y57>N(- - -)|t171>c,Y57>N(- - -)|g173>a,S58>N(- - -)|c174>t,S58>N(- - -)|g175>t,G59>Y(+ - -)|g176>a,G59>Y(+ - -)|t177>c,G59>Y(+ - -)|g184>a,G62>N(- - -)|g185>a,G62>N(- - -)|a187>g,S63>G(+ + -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|c192>a,S64>A(- + -)|a195>t|a197>t,Y66>F(- + -)|a204>t|a206>g,D69>G(- - -)|t208>c,S70>P(+ - -)|g213>a|g225>c|a232>g,I78>V(+ - +)|c237>t|a238>c|t246>a,N82>K(+ - -)|t247>g,S83>E(- - -)|c248>a,S83>E(- - -)|c249>a,S83>E(- - -)|a254>c,N85>T(- + -)|c257>t,T86>I(- - -)|g258>t,T86>I(- - -)|c259>t,L87>F(+ - -)|g261>t,L87>F(+ - -)|g267>a|a274>g,N92>D(+ + -)|g278>a,S93>N(- - -)|c280>g,L94>V(+ - +)|a285>g|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c299>g,A100>G(- + -)|a303>t|a308>t,Y103>F(- + -)|a317>g,K106>R(+ + +)| g80>a,G27>E(- - -)|t82>g,F28>D(- - -)|t83>a,F28>D(- - -)|c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|g104>a,S35>K(- - -)|c105>g,S35>K(- - -)|c108>t|a110>t,Y37>F(- + -)|t111>c,Y37>F(- + -)|c113>g,A38>G(- + -)|c114>a,A38>G(- + -)| a115>t,M39>L(+ + -)|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c129>t|t160>g,S54>A(- + -)|t164>g,V55>G(- - -)|t165>a,V55>G(- - -)| t168>a|t169>a,Y57>N(- - -)|t171>c,Y57>N(- - -)|g173>a,S58>N(- - -)|c174>t,S58>N(- - -)|g175>t,G59>Y(+ - -)|g176>a,G59>Y(+ - -)|t177>c,G59>Y(+ - -)|g184>a,G62>N(- - -)|g185>a,G62>N(- - -)|a187>g,S63>G(+ + -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|c192>a,S64>A(- + -)|a195>t| a197>t,Y66>F(- + -)|a204>t|a206>g,D69>G(- - -)|t208>c,S70>P(+ - -)|g213>a|g225>c|a232>g,I78>V(+ - +)|c237>t|a238>c|t246>a,N82>K(+ - -)|t247>g,S83>E(- - -)|c248>a,S83>E(- - -)|c249>a,S83>E(- - -)|a254>c,N85>T(- + -)|c257>t,T86>I(- - -)|g258>t,T86>I(- - -)|c259>t,L87>F(+ - -)|g261>t,L87>F(+ - -)|g267>a|a274>g,N92>D(+ + -)|g278>a,S93>N(- - -)|c280>g,L94>V(+ - +)|a285>g|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c299>g,A100>G(- + -)|a303>t|a308>t,Y103>F(- + -)| a317>g,K106>R(+ + +)|a320>t| 319 (320) 224 (225) 161 63 (64) 12 (13) 51 7 9 8 4 2 4 7 (8) 5 4 6 3 4 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 12 12 1 11 0 2 2 1 0 1 1 1 0 3 0 1 51 51 44 7 1 6 0 0 1 0 0 0 1 1 1 1 0 2 30 24 9 15 2 13 2 4 1 2 0 1 1 2 1 0 1 0 117 114 86 28 8 20 4 3 4 1 2 2 4 1 2 2 2 1 7 (8) 7 6 1 (2) 0 (1) 1 1 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|ta,243-244(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,243-244(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,184-187(CDR2)|agca,190-193(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gaagacatgtttaagagtttcgga ttgacgtgggtccgtcaggctccagggaaggggctggagtgggtcgcagga ataaacaattacaatggtgcaact ttctatgctggccccgtaaagggccgcttcaccgtctctcgagataaagaaaagaccattttttatctacaaatggacaacgtgagggtcgacgacacgggcgtttatttctgt gcgagagtcaggaatagtaattggtacttcgaccac ARVRNSNWYFDH IGHV3-23 IGHD3-3 IGHJ2 gaagacatgtttaagagtttcgga ttgacgtgggtccgtcaggctccagggaaggggctggagtgggtcgcagga ataaacaattacaatggtgcaact ttctatgctggccccgtaaagggccgcttcaccgtctctcgagataaagaaaagaccattttttatctacaaatggacaacgtgagggtcgacgacacgggcgtttatttctgt gcgagagtcaggaatagtaattggtacttcgaccac IGA1 62 213 27 35 17 34 10 28 9 3 26 24 JY8QFUQ01AS5EL 3429 productive Homsap IGHV4-4*07 F 1061 99.53 214/215 nt NA Homsap IGHJ2*01 F 229 92.45 49/53 nt Homsap IGHD4-11*01 ORF 2 8 7 13 8.7.13 [1.17.38.11] CARDFSNRGWYFDLW in-frame + tctctggtggctccatcagtagtttctactggagctggatccggcagcccgccgggaagggactggagtggattgggcgtatctataccagtgggagcaccaactacaacccctccctcaagagtcgagtcaccatgtcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgccgcggacacggccgtgtattactgtgcgagagacttcagtaacagaggatggtacttcgatctctggggccgtggcaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccc NA IGA2 100 100 [285] a110>t,Y37>F(- + -)| a110>t,Y37>F(- + -)| 320 223 222 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagtagtttctac tggagctggatccggcagcccgccgggaagggactggagtggattgggcgt atctataccagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatgtcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgccgcggacacggccgtgtattactgt gcgagagacttcagtaacagaggatggtacttcgatctc ARDFSNRGWYFDL IGHV4-4 IGHD4-11 IGHJ2 ggtggctccatcagtagtttctac tggagctggatccggcagcccgccgggaagggactggagtggattgggcgt atctataccagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatgtcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgccgcggacacggccgtgtattactgt gcgagagacttcagtaacagaggatggtacttcgatctc IGA2 1 210 0 1 0 0 0 1 0 0 0 1 JY8QFUQ01AS6B9 14512 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 963 94.01 204/217 nt NA Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD2-8*02 F 2 8 8 20 8.8.20 [1.17.38.11] CARDLWLCSGGECYHRGGFENW in-frame + ctctgggttcaccttcagtaactcctggatgcactgggtccgccaagctccagggaaggggccggagtgggtctcacgtattaatagtgatgggagtaatacaatctacgcggactccgtgaagggccgactcaccatctccagagacaacgccaaaaacacggtgtatctgcaattgaacagtctgagagccgaggacacggctgtgtactactgtgcaagagatctgtggctttgtagtggtggtgaatgctatcatcggggtggttttgagaactggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [308] a81>g|g107>a,S36>N(- - -)|a110>c,Y37>S(+ - -)|t149>c,L50>P(- - -)|t152>a,V51>E(- + -)|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)|g197>t,S66>I(- - -)|t226>c,F76>L(+ - -)|g252>a|c259>g,L87>V(+ - +)|a271>t,M91>L(+ + -)|t306>c| a81>g|g107>a,S36>N(- - -)|a110>c,Y37>S(+ - -)| t149>c,L50>P(- - -)|t152>a,V51>E(- + -)| g191>a,S64>N(- - -)|c192>t,S64>N(- - -)| g197>t,S66>I(- - -)|t226>c,F76>L(+ - -)|g252>a|c259>g,L87>V(+ - +)|a271>t,M91>L(+ + -)|t306>c| 320 225 212 13 3 10 1 3 1 3 1 0 1 1 0 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 1 1 0 0 1 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 0 1 0 0 0 1 0 0 0 0 30 24 22 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 117 114 108 6 2 4 0 1 0 2 0 0 1 0 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcaccttcagtaactcctgg atgcactgggtccgccaagctccagggaaggggccggagtgggtctcacgt attaatagtgatgggagtaataca atctacgcggactccgtgaagggccgactcaccatctccagagacaacgccaaaaacacggtgtatctgcaattgaacagtctgagagccgaggacacggctgtgtactactgt gcaagagatctgtggctttgtagtggtggtgaatgctatcatcggggtggttttgagaac ARDLWLCSGGECYHRGGFEN IGHV3-74 IGHD2-8 IGHJ3 gggttcaccttcagtaactcctgg atgcactgggtccgccaagctccagggaaggggccggagtgggtctcacgt attaatagtgatgggagtaataca atctacgcggactccgtgaagggccgactcaccatctccagagacaacgccaaaaacacggtgtatctgcaattgaacagtctgagagccgaggacacggctgtgtactactgt gcaagagatctgtggctttgtagtggtggtgaatgctatcatcggggtggttttgagaac IGA1 13 213 8 5 4 6 4 7 2 1 6 4 JY8QFUQ01AS9TS 19091 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 828 87.1 189/217 nt 86.64 188/217 nt Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD3-3*01 F 1 8 8 16 8.8.16 [1.17.38.11] CTKDPQQLLEGVNWFDPW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'G' (1 nucleotides at position 48 to 48 in the user submitted sequence), (cause frameshift); ctctggattcacctttaggagctatgccatgagttgggtccgccaggGctccagggaaggggctagagtgggtctcgtctatcagtggcagtggtgataagacaaagtatgcagattcagtgaggggccggttcaccatctccagagacaattacgacaacacattatatctgcgaatggagggcctgagagccgaggacacggccacatattactgtacgaaagatcctcagcaactgttggaaggggtaaattggttcgacccctggggccagggaaccctggtcaaggtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] c105>g,S35>R(- - -)|c120>t|g150>a|a162>g|g163>t,A55>S(- + -)|t168>c|t174>c|g188>a,G63>D(- - -)|g191>a,S64>K(- - -)|c192>g,S64>K(- - -)|t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|c201>t|c207>t|c210>a|a215>g,K72>R(+ + +)|c248>a,S83>Y(+ - -)|a250>g,K84>D(+ - -)|g252>c,K84>D(+ - -)|g258>a|c259>t|g261>a|a269>g,Q90>R(+ - -)|a274>g,N92>E(+ - -)|c276>g,N92>E(+ - -)|a277>g,S93>G(+ + -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g313>a,A105>T(- - -)| c105>g,S35>R(- - -)| c120>t|g150>a|a162>g|g163>t,A55>S(- + -)| t168>c|t174>c|g188>a,G63>D(- - -)|g191>a,S64>K(- - -)|c192>g,S64>K(- - -)| t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|c201>t|c207>t|c210>a|a215>g,K72>R(+ + +)|c248>a,S83>Y(+ - -)|a250>g,K84>D(+ - -)|g252>c,K84>D(+ - -)|g258>a|c259>t|g261>a|a269>g,Q90>R(+ - -)|a274>g,N92>E(+ - -)|c276>g,N92>E(+ - -)|a277>g,S93>G(+ + -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)| g313>a,A105>T(- - -)| 320 225 196 29 11 18 6 7 4 3 0 2 0 1 1 4 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 47 4 3 1 1 1 1 0 0 0 0 0 0 0 1 0 30 24 19 5 2 3 0 2 0 2 0 0 0 0 0 1 0 0 117 114 96 18 6 12 5 3 3 1 0 2 0 1 1 2 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaggagctatgcc atgagttgggtccgccaggctccagggaaggggctagagtgggtctcgtct atcagtggcagtggtgataagaca aagtatgcagattcagtgaggggccggttcaccatctccagagacaattacgacaacacattatatctgcgaatggagggcctgagagccgaggacacggccacatattactgt acgaaagatcctcagcaactgttggaaggggtaaattggttcgacccc TKDPQQLLEGVNWFDP IGHV3-23 IGHD3-3 IGHJ5 ggattcacctttaggagctatgcc atgagttgggtccgccaggctccagggaaggggctagagtgggtctcgtct atcagtggcagtggtgataagaca aagtatgcagattcagtgaggggccggttcaccatctccagagacaattacgacaacacattatatctgcgaatggagggcctgagagccgaggacacggccacatattactgt acgaaagatcctcagcaactgttggaaggggtaaattggttcgacccc IGA1 28 213 19 9 10 18 9 10 9 2 13 4 JY8QFUQ01ASBBA 17484 productive Homsap IGHV3-11*01 F 982 94.52 207/219 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD4-17*01 F 2 8 8 15 8.8.15 [2.17.38.11] CARRRDYGDYGDGFDYW in-frame - gcctctggattcatcttcagtgactactacatgacctggatccgccaggctccagggaaggggctggagtgggtttcatacattcgtagtaatgggagtcccatatacaacgcagactctgggaggggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaatagtctgagagtcgaggacacggccgtgtattactgtgcgagacggcgggactacggtgactacggtgacggctttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [295] c86>t,T29>I(- - -)|g119>c,S40>T(+ - +)|a169>c,S57>R(- - -)|g176>a,S59>N(- - -)|t186>g|a190>c,T64>P(+ + -)|t199>a,Y67>N(- - -)|t212>g,V71>G(- - -)|a215>g,K72>R(+ + +)|c276>t|c279>t|c287>t,A96>V(+ - +)| c86>t,T29>I(- - -)| g119>c,S40>T(+ - +)| a169>c,S57>R(- - -)|g176>a,S59>N(- - -)|t186>g|a190>c,T64>P(+ + -)| t199>a,Y67>N(- - -)|t212>g,V71>G(- - -)|a215>g,K72>R(+ + +)|c276>t|c279>t|c287>t,A96>V(+ - +)| g319>c|a320>g| 318 (320) 225 (227) 213 12 (14) 3 (5) 9 1 (2) 1 4 0 2 0 0 1 1 (2) 0 0 2 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 20 4 1 3 0 1 0 0 2 0 0 0 0 0 0 1 117 114 108 6 2 4 1 0 3 0 0 0 0 1 0 0 0 1 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcatcttcagtgactactac atgacctggatccgccaggctccagggaaggggctggagtgggtttcatac attcgtagtaatgggagtcccata tacaacgcagactctgggaggggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaatagtctgagagtcgaggacacggccgtgtattactgt gcgagacggcgggactacggtgactacggtgacggctttgactac ARRRDYGDYGDGFDY IGHV3-11 IGHD4-17 IGHJ4 ggattcatcttcagtgactactac atgacctggatccgccaggctccagggaaggggctggagtgggtttcatac attcgtagtaatgggagtcccata tacaacgcagactctgggaggggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaatagtctgagagtcgaggacacggccgtgtattactgt gcgagacggcgggactacggtgactacggtgacggctttgactac IGA1 12 213 6 6 5 6 1 6 2 1 5 4 JY8QFUQ01ASGTL 19943 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 801 85.71 186/217 nt 84.79 184/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-12*01 F 3 8 8 14 8.8.14 [1.17.38.11] CARDPGNYWGYYYDSW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'G' (1 nucleotides at position 58 to 58 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 55 of V-REGION: 'A' (1 nucleotides at position 79 to 79 in the user submitted sequence), (cause frameshift); gtctggattcacctttagtgattatgccttgagctgggtccgccagactcccgggaaGggggctggagtggatctcagAaaatcactggtgatggcggtaacaaatactatgcagactccgtgagggaccggttcaccatctccagagacaactccaagaatattttgtatctgcagatgagcagcctgagagccgaagacacggccatgtattactgtgcgagagatccgggaaattattggggatactattatgactcttggggcctgggaaccctggtcgccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [292] c75>g|c105>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|a115>t,M39>L(+ + -)|g133>a,A45>T(- - -)|a138>c|g157>a,V53>I(+ - +)|c164>a,A55>E(- - -)|t165>a,A55>E(- - -)|t168>c|g170>c,S57>T(+ - +)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|t186>c|g191>a,S64>N(- - -)|c194>a,T65>K(- - -)|c201>t|a215>g,K72>R(+ + +)|g221>a,G74>D(- - -)|t246>c|c255>t|c257>t,T86>I(- - -)|g258>t,T86>I(- - -)|c259>t|a270>g|a275>g,N92>S(- - -)|g291>a|g301>a,V101>M(+ - -)|a303>g,V101>M(+ - -)|a317>g,K106>R(+ + +)| c75>g| c105>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)| a115>t,M39>L(+ + -)|g133>a,A45>T(- - -)|a138>c|g157>a,V53>I(+ - +)|c164>a,A55>E(- - -)|t165>a,A55>E(- - -)| t168>c|g170>c,S57>T(+ - +)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|t186>c|g191>a,S64>N(- - -)|c194>a,T65>K(- - -)| c201>t|a215>g,K72>R(+ + +)|g221>a,G74>D(- - -)|t246>c|c255>t|c257>t,T86>I(- - -)|g258>t,T86>I(- - -)|c259>t|a270>g|a275>g,N92>S(- - -)|g291>a|g301>a,V101>M(+ - -)|a303>g,V101>M(+ - -)| a317>g,K106>R(+ + +)| 320 225 193 32 11 21 7 8 6 3 1 2 1 1 1 1 1 0 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 20 4 1 3 1 1 2 0 0 0 0 0 0 0 0 0 51 51 45 6 1 5 0 2 0 0 1 1 1 1 0 0 0 0 30 24 17 7 2 5 1 2 0 2 0 1 0 0 1 0 0 0 117 114 101 13 6 7 4 3 4 1 0 0 0 0 0 0 1 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcacctttagtgattatgcc ttgagctgggtccgccagactcccgggaaggggctggagtggatctcagaa atcactggtgatggcggtaacaaa tactatgcagactccgtgagggaccggttcaccatctccagagacaactccaagaatattttgtatctgcagatgagcagcctgagagccgaagacacggccatgtattactgt gcgagagatccgggaaattattggggatactattatgactct ARDPGNYWGYYYDS IGHV3-23 IGHD5-12 IGHJ4 ggattcacctttagtgattatgcc ttgagctgggtccgccagactcccgggaaggggctggagtggatctcagaa atcactggtgatggcggtaacaaa tactatgcagactccgtgagggaccggttcaccatctccagagacaactccaagaatattttgtatctgcagatgagcagcctgagagccgaagacacggccatgtattactgt gcgagagatccgggaaattattggggatactattatgactct IGA1 30 213 23 7 14 18 9 12 7 3 12 8 JY8QFUQ01ASIFV 16741 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 1000 95.43 209/219 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD2-15*01 F 3 8 8 15 8.8.15 [2.17.38.11] CAKLGVAATTWNFFDYW in-frame - gcctctggattcacctttaccacctatgccatgagctgggtccgccaggctccagggaaggggctggaatgggtctcaactattagtggtagtggtggcaggacatactacgcagactccgtgaagggccggttctccatctccagagacaattccaagaacacactatatctgcaaatgaacagcctgagagtcgaggacacggccgtatattactgtgcaaaattgggggtggcagctactacttggaacttctttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [296] g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|g153>a|g163>a,A55>T(- - -)|t189>c|c192>g,S64>R(- - -)|a229>t,T77>S(+ - +)|g258>a|g261>a|c287>t,A96>V(+ - +)|g315>a| g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)| g153>a|g163>a,A55>T(- - -)| t189>c|c192>g,S64>R(- - -)| a229>t,T77>S(+ - +)|g258>a|g261>a|c287>t,A96>V(+ - +)| g315>a|g319>t|a320>t| 318 (320) 225 (227) 214 11 (13) 5 (7) 6 0 5 1 1 0 0 1 (2) 0 2 1 0 (1) 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 0 0 2 0 0 0 51 51 49 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 30 24 22 2 1 1 0 0 0 1 0 0 0 0 0 1 0 0 117 114 110 4 2 2 0 2 1 0 0 0 1 0 0 0 0 0 6 (8) 6 5 1 (3) 1 (3) 0 0 1 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacctttaccacctatgcc atgagctgggtccgccaggctccagggaaggggctggaatgggtctcaact attagtggtagtggtggcaggaca tactacgcagactccgtgaagggccggttctccatctccagagacaattccaagaacacactatatctgcaaatgaacagcctgagagtcgaggacacggccgtatattactgt gcaaaattgggggtggcagctactacttggaacttctttgactac AKLGVAATTWNFFDY IGHV3-23 IGHD2-15 IGHJ4 ggattcacctttaccacctatgcc atgagctgggtccgccaggctccagggaaggggctggaatgggtctcaact attagtggtagtggtggcaggaca tactacgcagactccgtgaagggccggttctccatctccagagacaattccaagaacacactatatctgcaaatgaacagcctgagagtcgaggacacggccgtatattactgt gcaaaattgggggtggcagctactacttggaacttctttgactac IGG2 10 213 6 4 5 8 1 2 3 1 3 3 JY8QFUQ01ASKXZ 3019 productive (see comment) Homsap IGHV3-30*01 F, or Homsap IGHV3-30*03 F or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 935 93.02 200/215 nt 92.56 199/215 nt Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD5-24*01 ORF 1 8 7 16 8.7.16 [1.17.38.11] CARDELPWDTIEYYFDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 63 of V-REGION: 2 nucleotides (from position 96 in the user submitted sequence), (cause frameshift) ctctggattcaccttcagtacctattccatgcactgggtccgccaggctccaggcaaggggctagagtgggtggcagttatatcatatgatggaaaaaaagtattatggagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtacctgcaaatggacagcctgaggcctgaagacacggctgtatattactgtgccagagatgagctcccttgggatacaatagagtactactttgactactggggccagggaaccctggtcaccgtctcgtcagcatccccgaccagccccaaggtcttcccgctgagactctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 86 89 [294] g107>c,S36>T(+ - +)|g112>t,A38>S(- + -)|t114>c,A38>S(- + -)|t192>a,N64>K(+ - -)|a195>g|c198>t|c201>t|c203>g,A68>G(- + -)|c259>g,L87>V(+ - +)|t264>c|a274>g,N92>D(+ + -)|a285>g|g286>c,A96>P(- - -)|g291>a|g303>a|g315>c| g107>c,S36>T(+ - +)|g112>t,A38>S(- + -)|t114>c,A38>S(- + -)| t192>a,N64>K(+ - -)|a195>g| c198>t|c201>t|c203>g,A68>G(- + -)|c259>g,L87>V(+ - +)|t264>c|a274>g,N92>D(+ + -)|a285>g|g286>c,A96>P(- - -)|g291>a|g303>a| g315>c| 320 223 207 16 8 8 3 2 2 2 0 0 0 1 3 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 1 0 0 0 0 1 0 1 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 22 20 2 1 1 1 0 0 0 0 0 0 1 0 0 0 0 117 114 104 10 6 4 2 2 2 1 0 0 0 0 1 2 0 0 8 8 7 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtacctattcc atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcagtt atatcatatgatggaaaaaaag tattatggagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtacctgcaaatggacagcctgaggcctgaagacacggctgtatattactgt gccagagatgagctcccttgggatacaatagagtactactttgactac ARDELPWDTIEYYFDY IGHV3-30 IGHD5-24 IGHJ4 ggattcaccttcagtacctattcc atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcagtt atatcatatgatggaaaaaaag tattatggagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtacctgcaaatggacagcctgaggcctgaagacacggctgtatattactgt gccagagatgagctcccttgggatacaatagagtactactttgactac IGA1 15 211 9 6 4 9 5 6 6 1 4 4 JY8QFUQ01ASLW8 19513 productive (see comment) Homsap IGHV1-69*01 F, or Homsap IGHV1-69*12 F or Homsap IGHV1-69*13 (F) or Homsap IGHV1-69D*01 F 966 94.86 203/214 nt 94.39 202/214 nt Homsap IGHJ3*02 F 196 88 44/50 nt Homsap IGHD3-22*01 F 3 8 8 17 8.8.17 [0.17.38.11] CASLPGDLTQIQDGVFDIW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'G' (1 nucleotides at position 45 to 45 in the user submitted sequence), (cause frameshift); tggaggcaccttcgacagttatgttatcagctgggtgcgacaggGcccctggacaagggcttgagtggatgggcgggatcatccctatctttggttcggcaaagtatgcacagaagttccagggcagagtcaggattaccgcggacgaatccacgagcacggcctacatggagctgagcagcctgagatctgaggacacggccgtgtattactgtgccagtcttcccggggatcttactcaaattcaagacggtgtttttgatatctggggccaggggacaatggtcagtgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcgcccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 79 89 [297] a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c108>t|c113>t,A38>V(+ - +)|a162>c|a190>t,T64>S(+ - +)|a192>g,T64>S(+ - +)|c198>g,N66>K(+ - -)|c201>t|c230>g,T77>R(- - -)|a258>g|g315>c| a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c108>t|c113>t,A38>V(+ - +)| a162>c| a190>t,T64>S(+ - +)|a192>g,T64>S(+ - +)| c198>g,N66>K(+ - -)|c201>t|c230>g,T77>R(- - -)|a258>g| g315>c|a318>t,R106>S(- - -)|g319>c|a320>t| 317 (320) 219 (222) 207 12 (15) 5 (7) 7 (8) 3 1 3 0 1 0 1 (3) 0 1 (2) 2 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 2 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 117 114 110 4 2 2 1 0 1 0 0 0 0 0 0 2 0 0 5 (8) 5 4 1 (4) 1 (3) 0 (1) 0 0 0 0 0 0 0 (2) 0 1 (2) 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|aa,245-246(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|tt,184-185(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggca,82-85(CDR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agca,192-195(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggaggcaccttcgacagttatgtt atcagctgggtgcgacaggcccctggacaagggcttgagtggatgggcggg atcatccctatctttggttcggca aagtatgcacagaagttccagggcagagtcaggattaccgcggacgaatccacgagcacggcctacatggagctgagcagcctgagatctgaggacacggccgtgtattactgt gccagtcttcccggggatcttactcaaattcaagacggtgtttttgatatc ASLPGDLTQIQDGVFDI IGHV1-69 IGHD3-22 IGHJ3 ggaggcaccttcgacagttatgtt atcagctgggtgcgacaggcccctggacaagggcttgagtggatgggcggg atcatccctatctttggttcggca aagtatgcacagaagttccagggcagagtcaggattaccgcggacgaatccacgagcacggcctacatggagctgagcagcctgagatctgaggacacggccgtgtattactgt gccagtcttcccggggatcttactcaaattcaagacggtgtttttgatatc IGA1 11 213 7 4 4 6 3 5 3 1 2 5 JY8QFUQ01ASMJE 632 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 922 92.13 199/216 nt 91.67 198/216 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVRAGFGELSGFDCW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 54 of V-REGION: 'AG' (2 nucleotides at position 73 to 74 in the user submitted sequence), (cause frameshift); tctggattcacctttggttattatggcatgagctgggtccgccaagctccggggaggggctgggagtgggtcAGtctggtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [289] a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a138>g|a143>g,K48>R(+ + +)|g147>c|c148>t,L50>W(+ - -)|t149>g,L50>W(+ - -)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a138>g|a143>g,K48>R(+ + +)|g147>c|c148>t,L50>W(+ - -)|t149>g,L50>W(+ - -)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 223 (224) 205 18 (19) 4 (5) 14 3 3 2 1 1 (2) 0 2 0 3 1 1 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 51 46 5 2 3 2 0 1 0 0 0 0 0 1 0 0 1 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| tct ggattcacctttggttattatggc atgagctgggtccgccaagctccggggaggggctgggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgagctgggtccgccaagctccggggaggggctgggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 17 213 8 9 4 9 4 8 4 0 9 4 JY8QFUQ01ASMT6 5002 productive Homsap IGHV4-39*02 F 951 92.73 204/220 nt NA Homsap IGHJ4*02 F 141 77.08 37/48 nt Homsap IGHD4-17*01 F 3 10 7 12 10.7.12 [1.17.38.11] CASVTTVSMRMKYW in-frame + ctctggtggctccatcagcagtgatagttactactggggctggatccgccagtccccagggaaggggctggagtggattgggaatatctattatcgtgggagcacctattacaacccatccctcgagagtcggctcaccatgtcggtagacacgtccaggaacctcttctccctgaggctgagctctgtgaccgccgcagacacggctgtatattactgtgcgagcgtgactacggtgtctatgaggatgaaatactggggccggggaaccccggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 93 100 [287, 288] a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c133>t,P45>S(+ - -)|g164>a,S55>N(- - -)|a175>c,S59>R(- - -)|c198>t|g207>a|a214>g,K72>E(+ - -)|a225>g|g226>c,V76>L(+ - +)|a234>g,I78>M(+ + -)|c237>g|a251>g,K84>R(+ + +)|a257>t,H86>L(- - -)|a269>g,K90>R(+ + +)|g303>a| a103>g,S35>D(- - -)|g104>a,S35>D(- - -)| c133>t,P45>S(+ - -)|g164>a,S55>N(- - -)| a175>c,S59>R(- - -)| c198>t|g207>a|a214>g,K72>E(+ - -)|a225>g|g226>c,V76>L(+ - +)|a234>g,I78>M(+ + -)|c237>g|a251>g,K84>R(+ + +)|a257>t,H86>L(- - -)|a269>g,K90>R(+ + +)|g303>a| a318>c,R106>S(- - -)|a320>t| 317 (320) 225 (228) 209 (210) 16 (18) 5 (6) 11 (12) 6 4 2 0 1 (2) 0 1 (2) 0 1 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 21 20 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 117 114 103 11 5 6 5 2 1 0 0 0 1 0 1 1 0 0 5 (8) 5 5 (6) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtggctccatcagcagtgatagttactac tggggctggatccgccagtccccagggaaggggctggagtggattgggaat atctattatcgtgggagcacc tattacaacccatccctcgagagtcggctcaccatgtcggtagacacgtccaggaacctcttctccctgaggctgagctctgtgaccgccgcagacacggctgtatattactgt gcgagcgtgactacggtgtctatgaggatgaaatac ASVTTVSMRMKY IGHV4-39 IGHD4-17 IGHJ4 ggtggctccatcagcagtgatagttactac tggggctggatccgccagtccccagggaaggggctggagtggattgggaat atctattatcgtgggagcacc tattacaacccatccctcgagagtcggctcaccatgtcggtagacacgtccaggaacctcttctccctgaggctgagctctgtgaccgccgcagacacggctgtatattactgt gcgagcgtgactacggtgtctatgaggatgaaatac IGA2 16 216 12 4 6 8 6 8 5 0 8 3 JY8QFUQ01ASO1O 13391 productive Homsap IGHV3-66*02 F 926 92.56 199/215 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-15*01 F 2 8 7 17 8.7.17 [1.17.38.11] CSTPTYCRGGSCSQIFDYW in-frame + cctctggattcaccgtcagtaactaccacatgaggtgggtccgccaggctccagggaaggggctggagtgggtctcagttatttacagcggtggtagtacatattacatagactccgtgaagggccgattcaccatctcgagagacgattccgagaacacagtgtatcttcaaatgaagagcctgagagctgaggacacggctgtatattactgttcgacgcccacatattgtaggggcggtagctgttcccagatttttgactattggggccagggaaccctggtcaccgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [297] g107>a,S36>N(- - -)|a109>t,N37>Y(- - -)|t112>c,Y38>H(+ - -)|c120>g,S40>R(- - -)|t171>c|c192>t|c198>t|g202>a,A68>I(+ - +)|c203>t,A68>I(+ - +)|c237>g|a244>g,N82>D(+ + -)|a250>g,K84>E(+ - -)|g258>a|c259>g,L87>V(+ - +)|c276>g,N92>K(+ - -)|g303>a|g313>t,A105>S(- + -)| g107>a,S36>N(- - -)|a109>t,N37>Y(- - -)|t112>c,Y38>H(+ - -)| c120>g,S40>R(- - -)| t171>c|c192>t| c198>t|g202>a,A68>I(+ - +)|c203>t,A68>I(+ - +)|c237>g|a244>g,N82>D(+ + -)|a250>g,K84>E(+ - -)|g258>a|c259>g,L87>V(+ - +)|c276>g,N92>K(+ - -)|g303>a| g313>t,A105>S(- + -)|g317>c,R106>T(- - -)|a318>g,R106>T(- - -)| 316 (318) 219 (221) 202 17 (19) 6 11 (13) 2 (3) 4 3 2 0 0 1 0 0 (1) 4 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 1 0 0 1 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 21 19 2 2 0 0 0 1 1 0 0 0 0 0 0 0 0 117 114 104 10 4 6 2 3 2 0 0 0 0 0 0 3 0 0 4 (6) 4 3 1 (3) 0 1 (3) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 1 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccgtcagtaactaccac atgaggtgggtccgccaggctccagggaaggggctggagtgggtctcagtt atttacagcggtggtagtaca tattacatagactccgtgaagggccgattcaccatctcgagagacgattccgagaacacagtgtatcttcaaatgaagagcctgagagctgaggacacggctgtatattactgt tcgacgcccacatattgtaggggcggtagctgttcccagatttttgactat STPTYCRGGSCSQIFDY IGHV3-66 IGHD2-15 IGHJ4 ggattcaccgtcagtaactaccac atgaggtgggtccgccaggctccagggaaggggctggagtgggtctcagtt atttacagcggtggtagtaca tattacatagactccgtgaagggccgattcaccatctcgagagacgattccgagaacacagtgtatcttcaaatgaagagcctgagagctgaggacacggctgtatattactgt tcgacgcccacatattgtaggggcggtagctgttcccagatttttgactat IGA1 16 210 11 5 7 11 4 5 4 2 7 3 JY8QFUQ01ASOW0 12319 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 948 93.93 201/214 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD6-6*01 F 2 8 8 16 8.8.16 [0.17.38.11] CAFSRALTGKWIDYLDHW in-frame + tggattcatcttcagcaactactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtattactggtgatgggagtaacccaatctacgcggaccccgtgaagggtcgattcaccatctccagagacaacgccaagaacacactatatctgcaaatgaacagtctgagagtcgaggacacggctgtgtattactgtgcctttagtagagcactcacggggaagtggatcgactaccttgaccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [293] c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)|a170>c,N57>T(- + -)|a172>g,S58>G(+ + -)|g191>a,S64>N(- - -)|a193>c,T65>P(+ + -)|g197>t,S66>I(- - -)|t208>c,S70>P(+ - -)|c222>t|g258>a|g261>a|c287>t,A96>V(+ - +)| c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)| a170>c,N57>T(- + -)|a172>g,S58>G(+ + -)|g191>a,S64>N(- - -)|a193>c,T65>P(+ + -)| g197>t,S66>I(- - -)|t208>c,S70>P(+ - -)|c222>t|g258>a|g261>a|c287>t,A96>V(+ - +)| a315>c|a316>t,R106>F(- - -)|g317>t,R106>F(- - -)|a318>t,R106>F(- - -)|g319>a|a320>g| 314 (320) 216 (222) 203 13 (19) 4 (7) 9 (12) 1 (2) 4 (5) 3 2 2 (3) 0 0 (2) 0 0 0 1 (2) 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 1 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 1 0 0 2 0 0 0 0 0 0 0 117 114 108 6 3 3 0 2 2 1 0 0 0 0 0 0 1 0 2 (8) 2 2 0 (6) 0 (3) 0 (3) 0 (1) 0 (1) 0 0 0 (1) 0 0 (2) 0 0 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t ggattcatcttcagcaactactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attactggtgatgggagtaaccca atctacgcggaccccgtgaagggtcgattcaccatctccagagacaacgccaagaacacactatatctgcaaatgaacagtctgagagtcgaggacacggctgtgtattactgt gcctttagtagagcactcacggggaagtggatcgactaccttgaccac AFSRALTGKWIDYLDH IGHV3-74 IGHD6-6 IGHJ4 ggattcatcttcagcaactactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attactggtgatgggagtaaccca atctacgcggaccccgtgaagggtcgattcaccatctccagagacaacgccaagaacacactatatctgcaaatgaacagtctgagagtcgaggacacggctgtgtattactgt gcctttagtagagcactcacggggaagtggatcgactaccttgaccac IGA1 13 213 10 3 7 8 3 5 3 1 3 6 JY8QFUQ01ASS6V 9983 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 927 92.17 200/217 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD6-19*01 F 1 8 8 13 8.8.13 [1.17.38.11] CAKGYSSGWYDFDCW in-frame + ctctggattcacctttagtagttttgccatgacctgggtccgtcaggctccagggaaggggctggagtgggtctcaactattcattataatggtgataacacatactacgcagactccgtgaggggccgattcaccatctccagagacgattccaagaccacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaaggggtatagcagtggctggtacgactttgactgctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [288] c105>t|c108>t|a110>t,Y37>F(- + -)|g119>c,S40>T(+ - +)|c129>t|g163>a,A55>T(- - -)|a169>c,S57>H(+ - -)|g170>a,S57>H(+ - -)|g172>t,G58>Y(+ - -)|g173>a,G58>Y(+ - -)|g176>a,S59>N(- - -)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|a215>g,K72>R(+ + +)|g225>a|a244>g,N82>D(+ + -)|a254>c,N85>T(- + -)| c105>t|c108>t|a110>t,Y37>F(- + -)| g119>c,S40>T(+ - +)|c129>t|g163>a,A55>T(- - -)| a169>c,S57>H(+ - -)|g170>a,S57>H(+ - -)|g172>t,G58>Y(+ - -)|g173>a,G58>Y(+ - -)|g176>a,S59>N(- - -)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)| a215>g,K72>R(+ + +)|g225>a|a244>g,N82>D(+ + -)|a254>c,N85>T(- + -)| a318>g|a320>g| 317 (320) 222 (225) 205 (206) 17 (19) 4 (6) 13 2 (4) 7 3 0 2 0 1 0 1 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 2 0 0 0 1 0 0 0 0 0 51 51 48 3 1 2 0 1 1 0 0 0 0 0 1 0 0 0 30 24 17 7 0 7 0 5 0 0 1 0 0 0 0 0 1 0 117 114 110 4 1 3 2 1 0 0 1 0 0 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtagttttgcc atgacctgggtccgtcaggctccagggaaggggctggagtgggtctcaact attcattataatggtgataacaca tactacgcagactccgtgaggggccgattcaccatctccagagacgattccaagaccacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaggggtatagcagtggctggtacgactttgactgc AKGYSSGWYDFDC IGHV3-23 IGHD6-19 IGHJ4 ggattcacctttagtagttttgcc atgacctgggtccgtcaggctccagggaaggggctggagtgggtctcaact attcattataatggtgataacaca tactacgcagactccgtgaggggccgattcaccatctccagagacgattccaagaccacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaggggtatagcagtggctggtacgactttgactgc IGG1 17 213 12 5 10 12 2 5 2 2 5 8 JY8QFUQ01ASSCV 18926 productive Homsap IGHV3-7*01 F 995 95.41 208/218 nt NA Homsap IGHJ5*02 F 228 94.12 48/51 nt Homsap IGHD6-6*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVRSEYSSSNWFDPW in-frame + cctctggattcacctttagtaaccattggatgaactgggtccgccaggctccagggaaggggctggagtgggtggccaacataatgccagatggaggtgagaaattctatgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgtgagatctgagtatagtagttcaaactggttcgacccctggggccaggggaccctggtcaccgtctcctcagcatccccgaccagccccaagggtcttcccgctgagcctcgacagcacccccccaaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 71 89 [289, 291, 288] g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)|g119>a,S40>N(- - -)|a170>t,K57>M(- + -)|a173>c,Q58>P(- - -)|a187>g,S63>G(+ + -)|a197>t,Y66>F(- + -)|a225>g|a245>g,N82>S(- - -)|c265>t|c314>t,A105>V(+ - +)| g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)| g119>a,S40>N(- - -)| a170>t,K57>M(- + -)|a173>c,Q58>P(- - -)|a187>g,S63>G(+ + -)| a197>t,Y66>F(- + -)|a225>g|a245>g,N82>S(- - -)|c265>t| c314>t,A105>V(+ - +)|g319>t|a320>c| 318 (320) 224 (226) 213 11 (13) 2 (4) 9 3 2 2 1 1 (2) 0 2 0 0 0 0 (1) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 0 0 1 0 1 0 0 0 0 0 117 114 110 4 2 2 2 0 1 0 0 0 1 0 0 0 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagtaaccattgg atgaactgggtccgccaggctccagggaaggggctggagtgggtggccaac ataatgccagatggaggtgagaaa ttctatgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gtgagatctgagtatagtagttcaaactggttcgacccc VRSEYSSSNWFDP IGHV3-7 IGHD6-6 IGHJ5 ggattcacctttagtaaccattgg atgaactgggtccgccaggctccagggaaggggctggagtgggtggccaac ataatgccagatggaggtgagaaa ttctatgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gtgagatctgagtatagtagttcaaactggttcgacccc IGA2 10 213 7 3 3 3 4 7 2 0 3 5 JY8QFUQ01ASTYN 7333 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F (see comment) 750 83.64 179/214 nt 83.18 178/214 nt Homsap IGHJ3*01 F 205 90 45/50 nt Homsap IGHD6-25*01 F 1 8 7 15 8.7.15 [1.16.38.11] CARGTRYNNGLDAFDVW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (83,64% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR2-IMGT, from codon 50 of V-REGION: 1 nucleotides (from position 65 in the user submitted sequence), (cause frameshift) tctcgagtgcctccatgatcagttactattggacctggattcggcagcccccagggaagggactgagtggattggggacatctattcctttggaggcaccagatacaacccgtcccttggcagtcgagtctccatatcactggacacgtccaataatgagttctccctgcaactgaactctgtgaccgctgcggacacggccttatatcactgtgcgagggggacccggtataacaacggtcttgacgcttttgatgtctggggcccagggacagtggtcatcgtctcttcatcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [291] t78>g|g79>a,G27>S(+ + -)|g83>c,G28>A(- + -)|c90>g,I30>M(+ + -)|g104>t,S35>I(- - -)|t105>c,S35>I(- - -)|c114>t|g119>c,S40>T(+ - +)|c126>t|t163>g,Y55>D(- - -)|t165>c,Y55>D(- - -)|a173>c,Y58>S(+ - -)|a175>t,S59>F(- - -)|g176>t,S59>F(- - -)|g189>a|a190>g,S64>G(+ + -)|a197>g,N66>R(+ - -)|c198>a,N66>R(+ - -)|c207>g|c213>t|a214>g,K72>G(- - -)|a215>g,K72>G(- - -)|g216>c,K72>G(- - -)|a229>t,T77>S(+ - +)|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|g252>t,K84>N(+ - -)|c255>t|c256>g,Q86>E(+ + -)|a268>c,K90>Q(+ - -)|g270>a,K90>Q(+ - -)|g275>a,S92>N(- - -)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|t307>c,Y103>H(+ - -)| t78>g| g79>a,G27>S(+ + -)|g83>c,G28>A(- + -)|c90>g,I30>M(+ + -)|g104>t,S35>I(- - -)|t105>c,S35>I(- - -)|c114>t| g119>c,S40>T(+ - +)|c126>t|t163>g,Y55>D(- - -)|t165>c,Y55>D(- - -)| a173>c,Y58>S(+ - -)|a175>t,S59>F(- - -)|g176>t,S59>F(- - -)|g189>a|a190>g,S64>G(+ + -)| a197>g,N66>R(+ - -)|c198>a,N66>R(+ - -)|c207>g|c213>t|a214>g,K72>G(- - -)|a215>g,K72>G(- - -)|g216>c,K72>G(- - -)|a229>t,T77>S(+ - +)|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|g252>t,K84>N(+ - -)|c255>t|c256>g,Q86>E(+ + -)|a268>c,K90>Q(+ - -)|g270>a,K90>Q(+ - -)|g275>a,S92>N(- - -)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|t307>c,Y103>H(+ - -)| a318>g|a320>g| 317 (320) 219 (222) 184 (185) 35 (37) 7 (9) 28 5 (7) 5 4 3 2 1 2 0 4 3 4 2 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 36 24 18 6 1 5 0 1 1 1 0 0 0 0 1 1 1 0 51 50 46 4 1 3 0 0 1 1 0 0 0 0 1 0 0 1 30 21 16 5 1 4 1 1 0 0 1 0 1 0 0 0 1 0 117 114 95 19 3 16 4 3 2 1 1 1 1 0 2 2 2 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctcg agtgcctccatgatcagttactat tggacctggattcggcagcccccagggaagggactgagtggattggggac atctattcctttggaggcacc agatacaacccgtcccttggcagtcgagtctccatatcactggacacgtccaataatgagttctccctgcaactgaactctgtgaccgctgcggacacggccttatatcactgt gcgagggggacccggtataacaacggtcttgacgcttttgatgtc ARGTRYNNGLDAFDV IGHV4-59 IGHD6-25 IGHJ3 agtgcctccatgatcagttactat tggacctggattcggcagcccccagggaagggactgagtggattggggac atctattcctttggaggcacc agatacaacccgtcccttggcagtcgagtctccatatcactggacacgtccaataatgagttctccctgcaactgaactctgtgaccgctgcggacacggccttatatcactgt gcgagggggacccggtataacaacggtcttgacgcttttgatgtc IGG1 34 209 17 17 9 21 8 13 4 2 19 9 JY8QFUQ01ASUCZ 12938 productive Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 824 86.7 189/218 nt NA Homsap IGHJ5*02 F 237 96.08 49/51 nt Homsap IGHD4-17*01 F 3 8 8 17 8.8.17 [1.17.38.11] CARHSGALATGSQNWFDPW in-frame - cgtcgggatttaggtttgatgattttggcatgagctgggtccgccaagttccagggaagggcctggaatgggtctctggtagtgactggagtggtggaagaacaggttatggtgactctgtgaagggacgattcatcatctccagagacaacgccaagaactccctatatctacaaatgaacgatctgcgagccgatgacacggccgtctattattgtgcgcgacatagtggggcgctggctactgggtctcagaactggttcgacccctggggccggggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [300] c75>g|t78>g|c84>t|c86>g,T29>R(- - -)|c87>g,T29>R(- - -)|a110>t,Y37>F(- + -)|c134>t,A45>V(+ - +)|g147>c|g153>a|t167>g,I56>S(- - -)|a169>g,N57>D(+ + -)|t171>c,N57>D(+ + -)|a176>g,N59>S(- - -)|t189>a|c192>a,S64>R(- - -)|c203>g,A68>G(- + -)|a204>t,A68>G(- + -)|c222>a|c230>t,T77>I(- - -)|g261>a|g267>a|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|a283>c|g291>t,E97>D(+ - +)|t301>g,L101>V(+ - +)|g303>c,L101>V(+ - +)|c307>t,H103>Y(+ - -)|c309>t,H103>Y(+ - -)|a316>c| c75>g|t78>g| c84>t|c86>g,T29>R(- - -)|c87>g,T29>R(- - -)|a110>t,Y37>F(- + -)| c134>t,A45>V(+ - +)|g147>c|g153>a| t167>g,I56>S(- - -)|a169>g,N57>D(+ + -)|t171>c,N57>D(+ + -)|a176>g,N59>S(- - -)|t189>a|c192>a,S64>R(- - -)| c203>g,A68>G(- + -)|a204>t,A68>G(- + -)|c222>a|c230>t,T77>I(- - -)|g261>a|g267>a|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|a283>c|g291>t,E97>D(+ - +)|t301>g,L101>V(+ - +)|g303>c,L101>V(+ - +)|c307>t,H103>Y(+ - -)|c309>t,H103>Y(+ - -)| a316>c|g319>c| 318 (320) 224 (226) 194 (195) 30 (31) 11 (12) 19 3 4 5 1 2 2 2 1 2 (3) 4 1 3 78 5 3 2 2 0 0 0 0 0 0 0 0 0 0 1 0 1 36 24 20 4 1 3 0 0 1 0 0 0 1 0 0 2 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 0 1 0 0 0 30 24 18 6 1 5 2 0 0 1 0 1 0 1 0 0 0 1 117 114 100 14 4 10 1 3 3 0 1 1 1 0 1 1 1 1 6 (8) 6 5 (6) 1 (2) 1 (2) 0 0 0 0 0 1 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| cgtcg ggatttaggtttgatgattttggc atgagctgggtccgccaagttccagggaagggcctggaatgggtctctggt agtgactggagtggtggaagaaca ggttatggtgactctgtgaagggacgattcatcatctccagagacaacgccaagaactccctatatctacaaatgaacgatctgcgagccgatgacacggccgtctattattgt gcgcgacatagtggggcgctggctactgggtctcagaactggttcgacccc ARHSGALATGSQNWFDP IGHV3-20 IGHD4-17 IGHJ5 ggatttaggtttgatgattttggc atgagctgggtccgccaagttccagggaagggcctggaatgggtctctggt agtgactggagtggtggaagaaca ggttatggtgactctgtgaagggacgattcatcatctccagagacaacgccaagaactccctatatctacaaatgaacgatctgcgagccgatgacacggccgtctattattgt gcgcgacatagtggggcgctggctactgggtctcagaactggttcgacccc IGA1 27 213 13 14 9 17 4 10 6 2 11 8 JY8QFUQ01ASUXV 21877 productive (see comment) Homsap IGHV4-61*02 F 956 92.76 205/221 nt 92.31 204/221 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-19*01 F 1 10 7 14 10.7.14 [1.17.38.11] CAREPQSNAWYYFDHW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'A' (1 nucleotides at position 28 to 28 in the user submitted sequence), (cause frameshift); tctctggtggctccgtcaacagtggtaAttttctactggagctggatccggcagcccgccgggaagggactggagtggatagggcgtatctatgccagtgggagcaccaactacaacccctccctcaagagtcgaatcaccatatcagcagacacatccaagaatcagttctccctgaggctgagttctgtgaccgccgcagacacaggcgtttattattgtgcgagagaaccacaaagcaatgcctggtactactttgaccactggggccggggagccctggtcaccgtctccacagcctccaccaagggccccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG2 100 94 [297] a88>g,I30>V(+ - +)|g92>a,S31>N(- - -)|g107>t,S36>I(- - -)|a110>t,Y37>F(- + -)|t159>a|a172>g,T58>A(- - -)|g226>a,V76>I(+ - +)|t239>c,V80>A(+ - +)|g246>a|c255>t|a269>g,K90>R(+ + +)|c276>t|g297>a|c299>g,A100>G(- + -)|g303>t|c309>t| a88>g,I30>V(+ - +)|g92>a,S31>N(- - -)|g107>t,S36>I(- - -)|a110>t,Y37>F(- + -)| t159>a| a172>g,T58>A(- - -)| g226>a,V76>I(+ - +)|t239>c,V80>A(+ - +)|g246>a|c255>t|a269>g,K90>R(+ + +)|c276>t|g297>a|c299>g,A100>G(- + -)|g303>t|c309>t| 320 229 213 16 7 9 3 4 3 1 0 0 1 1 0 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 0 4 1 1 0 0 0 0 1 0 0 0 1 0 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 21 20 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 117 114 104 10 6 4 1 3 3 1 0 0 0 0 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcaacagtggtattttctac tggagctggatccggcagcccgccgggaagggactggagtggatagggcgt atctatgccagtgggagcacc aactacaacccctccctcaagagtcgaatcaccatatcagcagacacatccaagaatcagttctccctgaggctgagttctgtgaccgccgcagacacaggcgtttattattgt gcgagagaaccacaaagcaatgcctggtactactttgaccac AREPQSNAWYYFDH IGHV4-61 IGHD6-19 IGHJ4 ggtggctccgtcaacagtggtattttctac tggagctggatccggcagcccgccgggaagggactggagtggatagggcgt atctatgccagtgggagcacc aactacaacccctccctcaagagtcgaatcaccatatcagcagacacatccaagaatcagttctccctgaggctgagttctgtgaccgccgcagacacaggcgtttattattgt gcgagagaaccacaaagcaatgcctggtactactttgaccac IGG2 16 216 11 5 7 10 4 6 7 0 4 5 JY8QFUQ01ASVJP 7630 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 855 88.48 192/217 nt 88.02 191/217 nt Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD1-26*01 F 1 8 8 16 8.8.16 [1.17.38.11] CAKGIVGASMQADWFHPW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'T' (1 nucleotides at position 57 to 57 in the user submitted sequence), (cause frameshift); ctctggattcgtctttggcgactatgcgatgagctgggtccggcaggctccagggaTgggggctggagtgggtctcaagtattagtggtagtggtgtcagcacatactacgtgggctccgtgaagggccgcttcaccatctccagagacaattccaagaatgtgttgtatctgcaaatgaacggcctgagagtcgaggacacggccacatatcactgtgcgaaaggtatagtgggagcttctatgcaagccgactggttccacccctggggccaggggaccctgctcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctagtccagggcttcttccccca NA IGA1 93 95 [297] a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|a103>g,S35>G(+ + -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c114>g|c129>g|a143>g,K48>R(+ + +)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|g188>t,G63>V(- - -)|t189>c,G63>V(- - -)|c203>t,A68>V(+ - +)|a204>g,A68>V(+ - +)|a206>g,D69>G(- - -)|g225>c|c255>t|a256>g,T86>V(- - -)|c257>t,T86>V(- - -)|c259>t|a277>g,S93>G(+ + -)|c287>t,A96>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|t307>c,Y103>H(+ - -)| a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|a103>g,S35>G(+ + -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c114>g| c129>g|a143>g,K48>R(+ + +)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| g188>t,G63>V(- - -)|t189>c,G63>V(- - -)| c203>t,A68>V(+ - +)|a204>g,A68>V(+ - +)|a206>g,D69>G(- - -)|g225>c|c255>t|a256>g,T86>V(- - -)|c257>t,T86>V(- - -)|c259>t|a277>g,S93>G(+ + -)|c287>t,A96>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|t307>c,Y103>H(+ - -)| a320>g| 319 (320) 224 (225) 199 25 (26) 5 (6) 20 8 (9) 3 6 3 0 0 0 0 1 3 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 1 5 3 1 1 0 0 0 0 0 0 1 0 0 51 51 47 4 1 3 1 1 0 0 0 0 0 0 0 2 0 0 30 24 22 2 0 2 0 0 0 1 0 0 0 0 0 0 1 0 117 114 101 13 3 10 4 1 5 2 0 0 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcgtctttggcgactatgcg atgagctgggtccggcaggctccagggagggggctggagtgggtctcaagt attagtggtagtggtgtcagcaca tactacgtgggctccgtgaagggccgcttcaccatctccagagacaattccaagaatgtgttgtatctgcaaatgaacggcctgagagtcgaggacacggccacatatcactgt gcgaaaggtatagtgggagcttctatgcaagccgactggttccacccc AKGIVGASMQADWFHP IGHV3-23 IGHD1-26 IGHJ5 ggattcgtctttggcgactatgcg atgagctgggtccggcaggctccagggagggggctggagtgggtctcaagt attagtggtagtggtgtcagcaca tactacgtgggctccgtgaagggccgcttcaccatctccagagacaattccaagaatgtgttgtatctgcaaatgaacggcctgagagtcgaggacacggccacatatcactgt gcgaaaggtatagtgggagcttctatgcaagccgactggttccacccc IGA1 25 213 20 5 9 14 11 11 4 1 13 7 JY8QFUQ01ASZ0M 16900 productive (see comment) Homsap IGHV4-59*03 F 786 85.51 183/214 nt 84.58 181/214 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD2-21*02 F 3 8 7 15 8.7.15 [1.17.37.11] CARGRTVVTVVDYVDFW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 86 of V-REGION: 'C' (1 nucleotides at position 159 to 159 in the user submitted sequence), (cause frameshift); in FR3-IMGT, from codon 68 of V-REGION: 1 nucleotides (from position 109 in the user submitted sequence), (cause frameshift) tctctggtgactccattagtggttactattggacgtggatccgacagcgcccagggatgagcctggagtggattggacaagtccattacactgggagcaccaagtacagccctccctcaagagtcgagtcaccatttctgttgacatgtccaagaaccCaattcaccctcatcttgacctctgtggccgctgcggacacggccgtctattactgtgcgagaggacgaacggtggtgactgttgttgactacgttgacttttggggtcagggagtcctggtcaacgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [291] g83>a,G28>D(- - -)|c90>t|a106>g,S36>G(+ + -)|c114>t|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|g129>a|c134>g,P45>R(- - -)|a143>t,K48>M(- + -)|g145>a,G49>S(+ + -)|a147>c,G49>S(+ + -)|g162>a|t163>c,Y55>Q(- - -)|t165>a,Y55>Q(- - -)|a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|c198>g,N66>K(+ - -)|c204>g|a234>t|a237>t|a240>t|c245>t,T82>M(- - -)|t262>a,S88>T(+ - +)|g267>c|a269>t,K90>I(- + -)|g270>c,K90>I(- + -)|c271>t|g275>c,S92>T(+ - +)|a283>g,T95>A(- - -)|g303>c| g83>a,G28>D(- - -)|c90>t|a106>g,S36>G(+ + -)|c114>t| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|g129>a|c134>g,P45>R(- - -)|a143>t,K48>M(- + -)|g145>a,G49>S(+ + -)|a147>c,G49>S(+ + -)|g162>a|t163>c,Y55>Q(- - -)|t165>a,Y55>Q(- - -)| a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)| c198>g,N66>K(+ - -)|c204>g|a234>t|a237>t|a240>t|c245>t,T82>M(- - -)|t262>a,S88>T(+ - +)|g267>c|a269>t,K90>I(- + -)|g270>c,K90>I(- + -)|c271>t|g275>c,S92>T(+ - +)|a283>g,T95>A(- - -)|g303>c| 315 217 186 31 11 20 3 4 4 2 1 0 5 2 6 4 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 1 1 2 0 0 0 0 0 0 0 0 0 51 51 41 10 2 8 0 3 0 1 1 0 1 1 1 2 0 0 30 21 18 3 0 3 1 0 0 1 0 0 0 0 1 0 0 0 117 113 99 14 7 7 1 0 2 0 0 0 4 1 4 2 0 0 3 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,257-258(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccattagtggttactat tggacgtggatccgacagcgcccagggatgagcctggagtggattggacaa gtccattacactgggagcacc aagtacagccctccctcaagagtcgagtcaccatttctgttgacatgtccaagaaccaattcaccctcatcttgacctctgtggccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttgttgactacgttgacttt ARGRTVVTVVDYVDF IGHV4-59 IGHD2-21 IGHJ4 ggtgactccattagtggttactat tggacgtggatccgacagcgcccagggatgagcctggagtggattggacaa gtccattacactgggagcacc aagtacagccctccctcaagagtcgagtcaccatttctgttgacatgtccaagaaccaattcaccctcatcttgacctctgtggccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttgttgactacgttgacttt IGA1 31 209 13 18 8 18 5 13 9 2 15 5 JY8QFUQ01AT4NT 15495 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 868 88.89 192/216 nt 88.43 191/216 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 15 8.8.15 [1.17.37.11] CAKDASRYYYNSYCDNW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 103 of V-REGION: 1 nucleotides (from position 213 in the user submitted sequence), (cause frameshift) ctctggattcacctttaccaactacgccatgagctgggttcgccaggttccagggaaggggctggagtgggtctcacttattagtgttcgtggcgatgacaccttctatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtatctgcaaatggacatcctgaaacccgaggacacggccgtttattttgcgcgaaagatgcgtctcgatattattataacagttattgtgacaattggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [293] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|t111>c|c126>t|c134>t,A45>V(+ - +)|g163>c,A55>L(+ - +)|c164>t,A55>L(+ - +)|g173>t,G58>V(- - -)|a175>c,S59>R(- - -)|t186>c|g188>a,G63>D(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a195>c|a197>t,Y66>F(- + -)|c201>t|t246>c|a274>g,N92>D(+ + -)|g278>t,S93>I(- - -)|g284>a,R95>K(+ + +)|g286>c,A96>P(- - -)|a303>t|c309>t|t312>c| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|t111>c| c126>t|c134>t,A45>V(+ - +)|g163>c,A55>L(+ - +)|c164>t,A55>L(+ - +)| g173>t,G58>V(- - -)|a175>c,S59>R(- - -)|t186>c|g188>a,G63>D(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a195>c| a197>t,Y66>F(- + -)|c201>t|t246>c|a274>g,N92>D(+ + -)|g278>t,S93>I(- - -)|g284>a,R95>K(+ + +)|g286>c,A96>P(- - -)|a303>t|c309>t|t312>c| 320 224 200 24 9 15 2 4 5 4 2 0 2 0 3 0 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 0 1 0 0 0 0 1 0 0 0 51 51 47 4 1 3 0 0 3 0 0 0 0 0 1 0 0 0 30 24 17 7 2 5 1 2 0 1 2 0 0 0 0 0 1 0 117 113 103 10 5 5 1 1 2 2 0 0 2 0 1 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaccaactacgcc atgagctgggttcgccaggttccagggaaggggctggagtgggtctcactt attagtgttcgtggcgatgacacc ttctatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtatctgcaaatggacatcctgaaacccgaggacacggccgtttattttgc gcgaaagatgcgtctcgatattattataacagttattgtgacaat AKDASRYYYNSYCDN IGHV3-23 IGHD3-22 IGHJ4 ggattcacctttaccaactacgcc atgagctgggttcgccaggttccagggaaggggctggagtgggtctcactt attagtgttcgtggcgatgacacc ttctatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtatctgcaaatggacatcctgaaacccgaggacacggccgtttattttgc gcgaaagatgcgtctcgatattattataacagttattgtgacaat IGG1 24 212 15 9 9 14 6 10 6 3 8 7 JY8QFUQ01AT5TS 12566 productive Homsap IGHV1-24*01 F 993 96.26 206/214 nt NA Homsap IGHJ5*02 F 141 77.08 37/48 nt Homsap IGHD5-18*01 F 1 8 8 7 8.8.7 [0.17.38.11] CAIGAMATW in-frame - cggaaaaaccctcactgaagtatccatgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggaggatttgatcctgaagatggtgaaataatctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgtgcaataggagctatggccacctggggccagggaaccctggtcaccgtcgactcagtatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [266] t82>a,Y28>K(- - -)|c84>a,Y28>K(- - -)|t109>g,L37>V(+ - +)|t165>a|c194>t,T65>I(- - -)|g226>a,V76>I(+ - +)|a232>g,M78>V(+ - -)|g291>a|c317>t,T106>I(- - -)| t82>a,Y28>K(- - -)|c84>a,Y28>K(- - -)|t109>g,L37>V(+ - +)| t165>a| c194>t,T65>I(- - -)| g226>a,V76>I(+ - +)|a232>g,M78>V(+ - -)|g291>a| c317>t,T106>I(- - -)|a320>g| 319 (320) 221 (222) 212 9 (10) 2 (3) 7 1 (2) 2 2 0 0 1 0 2 0 0 0 1 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 0 0 1 0 1 0 0 0 1 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 117 114 111 3 1 2 1 2 0 0 0 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,14-15(FR1)|aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|aa,107-108(CDR1)|ta,110-111(CDR1)|aa,141-142(FR2)|aa,143-144(FR2)|aa,176-177(CDR2)|aa,191-192(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,249-250(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,14-15(FR1)|tt,74-75(FR1)|ta,82-83(CDR1)|tt,109-110(CDR1)|tt,149-150(FR2)|tt,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,249-250(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggta,12-15(FR1)|ggct,24-27(FR1)|ggtt,72-75(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,163-166(FR2-CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| c ggaaaaaccctcactgaagtatcc atgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggagga tttgatcctgaagatggtgaaata atctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcaataggagctatggccacc AIGAMAT IGHV1-24 IGHD5-18 IGHJ5 ggaaaaaccctcactgaagtatcc atgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggagga tttgatcctgaagatggtgaaata atctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcaataggagctatggccacc IGA1 8 213 4 4 3 4 1 4 2 0 2 4 JY8QFUQ01AT8HH 14240 productive Homsap IGHV4-31*02 F, or Homsap IGHV4-31*03 F or Homsap IGHV4-31*04 F 803 85.07 188/221 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD5-24*01 ORF 3 10 7 11 10.7.11 [1.17.38.11] CAGGEDPFKVRHW in-frame + tctctggtggctccttttctaatcatggttaccactgtagttggctccgccagttcccagggaaggacctggagtggattgcctacatctattacactgggagcaccgagtataacccgtccctcaggagtcgagttgccatatcagtggacacgtctgggaaccagtgttccctggagttgaactctgtgagtgccgcggacacggccgtctattactgtgcgggaggcgaggatcccttcaaagttcgacactggggccagggaacccgggtcaccgtctcctcagcatccccggaccagccccaagggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccga NA IGA2 100 89 [287] a88>t,I30>F(+ - -)|c90>t,I30>F(+ - -)|a91>t|g92>c|c93>t|g101>a,S34>N(- - -)|g103>c,G35>H(+ - -)|g104>a,G35>H(+ - -)|t112>c,Y38>H(+ - -)|g117>t,W39>C(+ - -)|c120>t|a124>c,I42>L(+ + +)|c133>t,H45>F(- - -)|a134>t,H45>F(- - -)|g146>a,G49>D(- - -)|g161>c,G54>A(- + -)|g162>c,G54>A(- + -)|g176>c,S59>T(+ - +)|t196>g,Y66>E(- - -)|c198>g,Y66>E(- - -)|c201>t|a215>g,K72>R(+ + +)|a229>g,T77>A(- - -)|a240>g|a250>g,K84>G(- - -)|a251>g,K84>G(- - -)|t260>g,F87>C(+ - -)|c261>t,F87>C(+ - -)|a268>g,K90>E(+ - -)|c271>t|g275>a,S92>N(- - -)|c284>g,T95>S(+ - +)|g303>c|a316>g,R106>G(- - -)| a88>t,I30>F(+ - -)|c90>t,I30>F(+ - -)|a91>t|g92>c|c93>t|g101>a,S34>N(- - -)|g103>c,G35>H(+ - -)|g104>a,G35>H(+ - -)|t112>c,Y38>H(+ - -)| g117>t,W39>C(+ - -)|c120>t|a124>c,I42>L(+ + +)|c133>t,H45>F(- - -)|a134>t,H45>F(- - -)|g146>a,G49>D(- - -)|g161>c,G54>A(- + -)|g162>c,G54>A(- + -)| g176>c,S59>T(+ - +)| t196>g,Y66>E(- - -)|c198>g,Y66>E(- - -)|c201>t|a215>g,K72>R(+ + +)|a229>g,T77>A(- - -)|a240>g|a250>g,K84>G(- - -)|a251>g,K84>G(- - -)|t260>g,F87>C(+ - -)|c261>t,F87>C(+ - -)|a268>g,K90>E(+ - -)|c271>t|g275>a,S92>N(- - -)|c284>g,T95>S(+ - +)|g303>c| a316>g,R106>G(- - -)|a320>g| 319 (320) 228 (229) 194 34 (35) 8 (9) 26 7 (8) 4 7 1 1 0 3 0 6 2 1 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 21 9 3 6 0 2 2 1 0 0 2 0 2 0 0 0 51 51 43 8 1 7 0 1 2 0 1 0 1 0 2 0 1 0 30 21 20 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 117 114 99 15 4 11 6 1 3 0 0 0 0 0 1 2 0 2 7 (8) 7 6 1 (2) 0 (1) 1 1 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,228-229(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,69-70(FR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,227-228(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|agca,131-134(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agtt,225-228(FR3)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,69-72(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|tacc,228-231(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tgcc,285-288(FR3)|tact,307-310(FR3)| tctct ggtggctccttttctaatcatggttaccac tgtagttggctccgccagttcccagggaaggacctggagtggattgcctac atctattacactgggagcacc gagtataacccgtccctcaggagtcgagttgccatatcagtggacacgtctgggaaccagtgttccctggagttgaactctgtgagtgccgcggacacggccgtctattactgt gcgggaggcgaggatcccttcaaagttcgacac AGGEDPFKVRH IGHV4-31 IGHD5-24 IGHJ4 ggtggctccttttctaatcatggttaccac tgtagttggctccgccagttcccagggaaggacctggagtggattgcctac atctattacactgggagcacc gagtataacccgtccctcaggagtcgagttgccatatcagtggacacgtctgggaaccagtgttccctggagttgaactctgtgagtgccgcggacacggccgtctattactgt gcgggaggcgaggatcccttcaaagttcgacac IGA2 33 216 18 15 11 20 7 13 5 3 18 7 JY8QFUQ01ATA76 4244 productive Homsap IGHV3-49*03 F, or Homsap IGHV3-49*05 F 1110 100 223/223 nt NA Homsap IGHJ4*02 F 114 70.83 34/48 nt Homsap IGHD4-17*01 F 3 8 10 12 8.10.12 [1.17.38.11] CTKCSVTIYFLTTG in-frame + ttctggattcacctttggtgattatgctatgagctggttccgccaggctccagggaaggggctggagtgggtaggtttcattagaagcaaagcttatggtgggacaacagaatacgccgcgtctgtgaaaggcagattcaccatctcaagagatgattccaaaagcatcgcctatctgcaaatgaacagcctgaaaaccgaggacacagccgtgtattactgtactaagtgttcggtgactatttactttttgactactggggccagggtaaccctggtcaccgtctcctcagcttccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG3 100 100 [291] g317>a,R106>K(+ + +)|a318>g,R106>K(+ + +)|g319>t|a320>g| 316 (320) 227 (231) 227 0 (4) 0 (2) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 0 0 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,158-159(FR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,181-182(CDR2)|aa,192-193(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|ta,69-70(FR1)|tt,75-76(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,124-125(FR2)|ta,158-159(FR2)|tt,162-163(FR2)|tt,167-168(CDR2)|tt,180-181(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agct,72-75(FR1)|agct,118-121(FR2)|ggtt,122-125(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggta,156-159(FR2)|ggtt,160-163(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|ggca,220-223(FR3)|agca,253-256(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,72-75(FR1)|tgct,111-114(CDR1)|agct,118-121(FR2)|agct,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| ttct ggattcacctttggtgattatgct atgagctggttccgccaggctccagggaaggggctggagtgggtaggtttc attagaagcaaagcttatggtgggacaaca gaatacgccgcgtctgtgaaaggcagattcaccatctcaagagatgattccaaaagcatcgcctatctgcaaatgaacagcctgaaaaccgaggacacagccgtgtattactgt actaagtgttcggtgactatttactttttgactact TKCSVTIYFLTT IGHV3-49 IGHD4-17 IGHJ4 ggattcacctttggtgattatgct atgagctggttccgccaggctccagggaaggggctggagtgggtaggtttc attagaagcaaagcttatggtgggacaaca gaatacgccgcgtctgtgaaaggcagattcaccatctcaagagatgattccaaaagcatcgcctatctgcaaatgaacagcctgaaaaccgaggacacagccgtgtattactgt actaagtgttcggtgactatttactttttgactact IGG3 0 219 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01ATDOT 16159 productive (see comment) Homsap IGHV3-11*01 F 939 93.46 200/214 nt 92.99 199/214 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-15*01 F 2 8 7 18 8.7.18 [1.17.38.11] CARDGPPYLYGSHGNSFDQW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 63 of V-REGION: 3 nucleotides (from position 95 in the user submitted sequence), (do not cause frameshift) ctctggattcagcttcagtgactactacatgagttgggtccgccaggctccagggaagggactggagtgggtttcatgcatcactactagtggtaccacattctacacggactctgtgaggggccgattcaccatgtccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtgtattactgtgcgagagatggcccaccttatttatatggaagtcatggtaattcctttgaccagtggggccagggaaccctagtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctggggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG3 93 100 [300] c86>g,T29>S(+ - +)|c120>t|a124>g,I42>V(+ - +)|g147>a|a164>g,Y55>C(- - -)|t168>c|g170>c,S57>T(+ - +)|g173>c,S58>T(+ - +)|t194>c,I65>T(- - -)|a197>t,Y66>F(- + -)|g202>a,A68>T(- - -)|a204>g,A68>T(- - -)|a215>g,K72>R(+ + +)|c234>g,I78>M(+ + -)| c86>g,T29>S(+ - +)| c120>t|a124>g,I42>V(+ - +)|g147>a|a164>g,Y55>C(- - -)| t168>c|g170>c,S57>T(+ - +)|g173>c,S58>T(+ - +)|t194>c,I65>T(- - -)| a197>t,Y66>F(- + -)|g202>a,A68>T(- - -)|a204>g,A68>T(- - -)|a215>g,K72>R(+ + +)|c234>g,I78>M(+ + -)| 320 222 208 14 3 11 4 2 1 2 0 0 1 0 2 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 47 4 2 2 2 1 1 0 0 0 0 0 0 0 0 0 30 21 17 4 1 3 0 0 0 2 0 0 0 0 2 0 0 0 117 114 109 5 0 5 2 1 0 0 0 0 1 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagcttcagtgactactac atgagttgggtccgccaggctccagggaagggactggagtgggtttcatgc atcactactagtggtaccaca ttctacacggactctgtgaggggccgattcaccatgtccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtgtattactgt gcgagagatggcccaccttatttatatggaagtcatggtaattcctttgaccag ARDGPPYLYGSHGNSFDQ IGHV3-11 IGHD2-15 IGHJ4 ggattcagcttcagtgactactac atgagttgggtccgccaggctccagggaagggactggagtgggtttcatgc atcactactagtggtaccaca ttctacacggactctgtgaggggccgattcaccatgtccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtgtattactgt gcgagagatggcccaccttatttatatggaagtcatggtaattcctttgaccag IGG3 14 210 9 5 3 7 6 7 2 1 7 4 JY8QFUQ01ATIZV 4824 productive Homsap IGHV3-66*02 F 840 88.32 189/214 nt NA Homsap IGHJ6*02 F (see comment) 197 80.33 49/61 nt Homsap IGHD2-8*01 F 3 8 7 11 8.7.11 [1.17.38.11] CASHLMVYALDVW in-frame + Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ctctggattcaccgtcagtggcaagtacatgagctgggtccgccaggctccagggcagggactggagtgggtctcagttatctatagtactggtagtacatactacgcagattccgtgaaagggcggttcaccatctccagagacagttccaacaacactctatatcttcaaatttacggcctgagagctgacgacacggctacttactactgtgcgagtcacttaatggtgtatgctctggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagcccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 95 [277, 278] a106>g,S36>G(+ + -)|c111>g,N37>K(+ - -)|a142>c,K48>Q(+ - -)|g147>a|t168>c|c174>t|g175>a,G59>T(+ - -)|g176>c,G59>T(+ - -)|c192>t|c207>t|g216>a|c222>g|a225>g|a245>g,N82>S(- - -)|g252>c,K84>N(+ - -)|g258>t|g261>a|g273>t,M91>I(+ + -)|a274>t,N92>Y(- - -)|a277>g,S93>G(+ + -)|g291>c,E97>D(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|t306>c| a106>g,S36>G(+ + -)|c111>g,N37>K(+ - -)| a142>c,K48>Q(+ - -)|g147>a| t168>c|c174>t|g175>a,G59>T(+ - -)|g176>c,G59>T(+ - -)|c192>t| c207>t|g216>a|c222>g|a225>g|a245>g,N82>S(- - -)|g252>c,K84>N(+ - -)|g258>t|g261>a|g273>t,M91>I(+ + -)|a274>t,N92>Y(- - -)|a277>g,S93>G(+ + -)|g291>c,E97>D(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|t306>c| a318>t,R106>S(- - -)| 317 (318) 219 (220) 194 25 (26) 11 14 (15) 4 5 3 3 1 0 1 (2) 0 3 2 3 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 1 0 0 51 51 49 2 1 1 0 1 0 0 1 0 0 0 0 0 0 0 30 21 16 5 3 2 0 1 2 1 0 0 0 0 1 0 0 0 117 114 98 16 7 9 3 3 1 2 0 0 1 0 2 1 3 0 5 (6) 5 5 0 (1) 0 0 (1) 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccgtcagtggcaagtac atgagctgggtccgccaggctccagggcagggactggagtgggtctcagtt atctatagtactggtagtaca tactacgcagattccgtgaaagggcggttcaccatctccagagacagttccaacaacactctatatcttcaaatttacggcctgagagctgacgacacggctacttactactgt gcgagtcacttaatggtgtatgctctggacgtc ASHLMVYALDV IGHV3-66 IGHD2-8 IGHJ6 ggattcaccgtcagtggcaagtac atgagctgggtccgccaggctccagggcagggactggagtgggtctcagtt atctatagtactggtagtaca tactacgcagattccgtgaaagggcggttcaccatctccagagacagttccaacaacactctatatcttcaaatttacggcctgagagctgacgacacggctacttactactgt gcgagtcacttaatggtgtatgctctggacgtc IGA2 25 210 15 10 8 16 7 9 8 3 10 4 JY8QFUQ01ATJ6D 5579 productive (see comment) Homsap IGHV3-11*01 F 1004 95.87 209/218 nt 94.95 207/218 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD5-18*01 F 3 8 8 11 8.8.11 [1.17.38.11] CARGYSYGCFDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'T' (1 nucleotides at position 59 to 59 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 59 of V-REGION: 'GGAGGGACT' (9 nucleotides at position 91 to 99 in the user submitted sequence), (do not cause frameshift); cctctggattcaccttcagtgactactacatgaactggatccgccaggctccagggaaTggggctggagtggatttcaaacatcaatagtGGAGGGACTagtgggaggaccatatattacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgagaggatacagctatggctgctttgactactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttc NA IGA1 100 100 [292] g119>a,S40>N(- - -)|g157>a,V53>I(+ - +)|t163>a,Y55>N(- - -)|t168>c|g170>a,S57>N(- - -)|t186>g|t189>g,S63>R(- - -)|c198>t|g303>a| g119>a,S40>N(- - -)|g157>a,V53>I(+ - +)|t163>a,Y55>N(- - -)| t168>c|g170>a,S57>N(- - -)|t186>g|t189>g,S63>R(- - -)| c198>t|g303>a| a320>g| 319 (320) 225 (226) 216 9 (10) 4 (5) 5 0 (1) 4 1 1 0 0 0 1 0 0 0 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 48 3 0 3 0 2 0 0 0 0 0 1 0 0 0 0 30 24 20 4 2 2 0 1 0 1 0 0 0 0 0 0 0 2 117 114 112 2 2 0 0 1 1 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtgactactac atgaactggatccgccaggctccagggaaggggctggagtggatttcaaac atcaatagtagtgggaggaccata tattacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagaggatacagctatggctgctttgactac ARGYSYGCFDY IGHV3-11 IGHD5-18 IGHJ4 ggattcaccttcagtgactactac atgaactggatccgccaggctccagggaaggggctggagtggatttcaaac atcaatagtagtgggaggaccata tattacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagaggatacagctatggctgctttgactac IGA1 9 213 6 3 5 5 1 4 2 2 3 2 JY8QFUQ01ATK2N 16759 productive Homsap IGHV3-66*02 F 858 89.25 191/214 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-21*02 F 3 8 7 12 8.7.12 [1.17.38.11] CARQTAPFGYFEDW in-frame + ctcggaattctccgtcagtgacagtcacatgagttgggtccgccaggctccagggaaggggctggagtgggtctcagttatttatagcggtggtagtgcatattatgcagactccgtgaggggccgattcaccatcgccagagacaaatctaagaacgtgttgtatcttcaaatgaacagtctgagacctgaggacacggctgtgtactactgtgcgagacagactgccccttttgggtactttgaggactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcc NA IGA1 100 100 [281] t78>g|g80>a,G27>E(- - -)|a85>t,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>g,N37>S(- - -)|c111>t,N37>S(- - -)|t112>c,Y38>H(+ - -)|c120>t|c192>t|a193>g,T65>A(- - -)|c198>t|c201>t|a215>g,K72>R(+ + +)|t235>g,S79>A(- + -)|t246>a,N82>K(+ - -)|c249>t|a256>g,T86>V(- - -)|c257>t,T86>V(- - -)|c259>t|c279>t|g286>c,A96>P(- - -)|t306>c| t78>g| g80>a,G27>E(- - -)|a85>t,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>g,N37>S(- - -)|c111>t,N37>S(- - -)|t112>c,Y38>H(+ - -)| c120>t| c192>t|a193>g,T65>A(- - -)| c198>t|c201>t|a215>g,K72>R(+ + +)|t235>g,S79>A(- + -)|t246>a,N82>K(+ - -)|c249>t|a256>g,T86>V(- - -)|c257>t,T86>V(- - -)|c259>t|c279>t|g286>c,A96>P(- - -)|t306>c| 318 220 197 23 9 14 5 2 9 2 0 0 1 1 1 0 0 2 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 36 24 17 7 0 7 2 2 1 1 0 0 1 0 0 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 21 19 2 1 1 1 0 1 0 0 0 0 0 0 0 0 0 117 114 102 12 6 6 2 0 6 1 0 0 0 1 1 0 0 1 6 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctcg gaattctccgtcagtgacagtcac atgagttgggtccgccaggctccagggaaggggctggagtgggtctcagtt atttatagcggtggtagtgca tattatgcagactccgtgaggggccgattcaccatcgccagagacaaatctaagaacgtgttgtatcttcaaatgaacagtctgagacctgaggacacggctgtgtactactgt gcgagacagactgccccttttgggtactttgaggac ARQTAPFGYFED IGHV3-66 IGHD2-21 IGHJ4 gaattctccgtcagtgacagtcac atgagttgggtccgccaggctccagggaaggggctggagtgggtctcagtt atttatagcggtggtagtgca tattatgcagactccgtgaggggccgattcaccatcgccagagacaaatctaagaacgtgttgtatcttcaaatgaacagtctgagacctgaggacacggctgtgtactactgt gcgagacagactgccccttttgggtactttgaggac IGA1 22 210 18 4 11 12 7 10 7 1 6 8 JY8QFUQ01ATP88 9503 productive (see comment) Homsap IGHV4-34*10 F 818 86.98 187/215 nt 86.51 186/215 nt Homsap IGHJ4*02 F 240 100 48/48 nt Homsap IGHD4-23*01 ORF 2 8 7 16 8.7.16 [1.17.38.11] CARNTNYGGNSGDYFDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 58 to 58 in the user submitted sequence), (cause frameshift); tgtctggtgagtccttcactaattactactggagctggatccgccagtccccaggaaAagggtctggagtggcttggggaggtccatcatagtggacgcaccgactacaacccgtccctcaagagtcgaatcaccatgtcgttagacacgtccgaaaatcagttctccctgaagttgacttctttgaccgccgcggacacggcagtatattattgtgcgagaaataccaactatggtggcaattccggggactactttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [296] c75>g|a77>c,Y26>S(+ - -)|g83>a,G28>E(- - -)|g104>c,S35>T(+ - +)|g106>a,G36>N(- - -)|g107>a,G36>N(- - -)|c133>t,P45>S(+ - -)|g141>a|a147>t|a157>c,I53>L(+ + +)|a165>g|a166>g,I56>V(+ - +)|a169>c,N57>H(- - -)|a190>c,S64>R(- - -)|a196>g,N66>D(+ + -)|a237>g|g238>t,V80>L(+ - +)|a250>g,K84>E(+ - -)|g252>a,K84>E(+ - -)|c255>t|a263>c,Y88>S(+ - -)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g280>t,V94>L(+ - +)|c300>a|g303>a|c309>t| c75>g|a77>c,Y26>S(+ - -)| g83>a,G28>E(- - -)|g104>c,S35>T(+ - +)|g106>a,G36>N(- - -)|g107>a,G36>N(- - -)| c133>t,P45>S(+ - -)|g141>a|a147>t|a157>c,I53>L(+ + +)|a165>g| a166>g,I56>V(+ - +)|a169>c,N57>H(- - -)|a190>c,S64>R(- - -)| a196>g,N66>D(+ + -)|a237>g|g238>t,V80>L(+ - +)|a250>g,K84>E(+ - -)|g252>a,K84>E(+ - -)|c255>t|a263>c,Y88>S(+ - -)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g280>t,V94>L(+ - +)|c300>a|g303>a|c309>t| t319>a| 318 (320) 221 (223) 193 (194) 28 (29) 10 (11) 18 5 6 5 0 5 1 1 0 (1) 2 1 2 0 78 5 3 2 1 1 0 0 0 0 1 0 0 0 0 1 0 0 36 24 20 4 0 4 0 3 0 0 0 0 0 0 1 0 0 0 51 51 46 5 3 2 1 1 1 0 1 0 1 0 0 0 0 0 30 21 18 3 0 3 1 0 0 0 2 0 0 0 0 0 0 0 117 114 100 14 6 8 3 2 4 0 1 1 0 0 1 0 2 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 (1) 0 0 0 0 aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,319-320(CDR3)| tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,319-320(CDR3)| agct,8-11(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|tacc,262-265(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tgtct ggtgagtccttcactaattactac tggagctggatccgccagtccccaggaaagggtctggagtggcttggggag gtccatcatagtggacgcacc gactacaacccgtccctcaagagtcgaatcaccatgtcgttagacacgtccgaaaatcagttctccctgaagttgacttctttgaccgccgcggacacggcagtatattattgt gcgagaaataccaactatggtggcaattccggggactactttgactac ARNTNYGGNSGDYFDY IGHV4-34 IGHD4-23 IGHJ4 ggtgagtccttcactaattactac tggagctggatccgccagtccccaggaaagggtctggagtggcttggggag gtccatcatagtggacgcacc gactacaacccgtccctcaagagtcgaatcaccatgtcgttagacacgtccgaaaatcagttctccctgaagttgacttctttgaccgccgcggacacggcagtatattattgt gcgagaaataccaactatggtggcaattccggggactactttgactac IGG1 26 210 16 10 11 16 5 10 9 0 10 7 JY8QFUQ01ATQIC 16336 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 991 94.98 208/219 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-8*01 F 2 8 8 8 8.8.8 [2.17.38.11] CARDNYGDYW in-frame - gcctctggattcactttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtgttaatggtgatgggggtaggacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcaccgtctcctctgcatccccgaccagccccaaggtcttcccgctgagcctctacagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 86 100 [274] c87>t|a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| c87>t| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| 320 227 216 11 5 6 4 1 1 0 0 0 0 0 3 1 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 3 0 0 0 0 0 0 0 0 1 0 0 117 114 108 6 4 2 1 1 0 0 0 0 0 0 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcactttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcactttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac IGA1 11 213 6 5 2 7 4 4 4 1 2 4 JY8QFUQ01ATVSF 15671 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 972 94.47 205/217 nt 94.01 204/217 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-3*01 F 1 8 8 10 8.8.10 [1.17.37.11] CARGGLEPVDYW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 66 of V-REGION: 1 nucleotides (from position 106 in the user submitted sequence), (cause frameshift) cctctggattcaccttcagtacatactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcccgtatcaatcctgatgggcgaatcacaactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggactcggctgtgtactactgtgcaaggggtggtttggagcccgtagattattggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [279] g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|a162>c|t168>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)|g191>t,S64>I(- - -)|g216>t,K72>N(+ - -)|a295>t,T99>S(+ - +)|t306>c| g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)| a162>c| t168>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)|g191>t,S64>I(- - -)| g216>t,K72>N(+ - -)|a295>t,T99>S(+ - +)|t306>c| a318>g|a320>g| 317 (320) 222 (225) 210 (211) 12 (14) 3 (5) 9 0 (2) 0 0 2 3 1 1 1 2 0 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 1 0 0 1 0 0 0 51 51 50 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 30 24 18 6 1 5 0 0 0 1 2 0 0 1 1 0 1 0 117 113 110 3 1 2 0 0 0 1 0 0 1 0 0 0 1 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtacatactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcccgt atcaatcctgatgggcgaatcaca actacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggactcggctgtgtactactgt gcaaggggtggtttggagcccgtagattat ARGGLEPVDY IGHV3-74 IGHD3-3 IGHJ4 ggattcaccttcagtacatactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcccgt atcaatcctgatgggcgaatcaca actacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggactcggctgtgtactactgt gcaaggggtggtttggagcccgtagattat IGG2 12 212 2 10 0 5 2 7 2 1 2 7 JY8QFUQ01ATWCJ 13618 productive (see comment) Homsap IGHV3-30*01 F 868 89.35 193/216 nt 88.89 192/216 nt Homsap IGHJ3*01 F 214 92 46/50 nt Homsap IGHD2-2*02 F 2 8 7 11 8.7.11 [1.17.38.11] CACGHMRDGYDVW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 64 of V-REGION: 1 nucleotides (from position 98 in the user submitted sequence), (cause frameshift) ctctggattcaccttcagtgcctttactatgcactgggtccgccaggctccaggcgagggactagagtgggtggcagctatatcatatgatggcagtaaaaatactatgcggactttgtgaagggccgattcaccatctccagagacaatcccaagagtacactgtatctacaaatgaacggcctgggaggtgatgacacggctttgtattactgtgcgtgcggccatatgcgggatggttatgatgtctggggccaagggacaaaggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [280] a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a110>t,Y37>F(- + -)|g112>a,A38>T(- - -)|a142>g,K48>E(+ - -)|g147>a|t164>c,V55>A(+ - +)|a186>c|t192>a|c201>t|a204>g|c209>t,S70>F(- - -)|c210>t,S70>F(- - -)|t247>c,S83>P(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|g267>a|a277>g,S93>G(+ + -)|a283>g,R95>G(- - -)|c287>g,A96>G(- + -)|g291>t,E97>D(+ - +)|g301>t,V101>L(+ - +)| a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a110>t,Y37>F(- + -)|g112>a,A38>T(- - -)| a142>g,K48>E(+ - -)|g147>a|t164>c,V55>A(+ - +)| a186>c|t192>a| c201>t|a204>g|c209>t,S70>F(- - -)|c210>t,S70>F(- - -)|t247>c,S83>P(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|g267>a|a277>g,S93>G(+ + -)|a283>g,R95>G(- - -)|c287>g,A96>G(- + -)|g291>t,E97>D(+ - +)|g301>t,V101>L(+ - +)| a316>t,R106>C(- - -)|a318>c,R106>C(- - -)|a320>g| 315 (320) 219 (224) 196 (198) 23 (26) 7 (8) 16 (18) 6 (7) 4 4 2 1 (2) 0 1 (2) 1 1 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 0 0 0 0 1 0 1 0 0 0 51 51 48 3 1 2 1 1 0 1 0 0 0 0 0 0 0 0 30 23 21 2 2 0 0 0 0 0 1 0 0 1 0 0 0 0 117 114 100 14 4 10 4 2 4 1 0 0 0 0 0 1 2 0 3 (8) 3 3 (5) 0 (3) 0 (1) 0 (2) 0 (1) 0 0 0 0 (1) 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgcctttact atgcactgggtccgccaggctccaggcgagggactagagtgggtggcagct atatcatatgatggcagtaaaaa tactatgcggactttgtgaagggccgattcaccatctccagagacaatcccaagagtacactgtatctacaaatgaacggcctgggaggtgatgacacggctttgtattactgt gcgtgcggccatatgcgggatggttatgatgtc ACGHMRDGYDV IGHV3-30 IGHD2-2 IGHJ3 ggattcaccttcagtgcctttact atgcactgggtccgccaggctccaggcgagggactagagtgggtggcagct atatcatatgatggcagtaaaaa tactatgcggactttgtgaagggccgattcaccatctccagagacaatcccaagagtacactgtatctacaaatgaacggcctgggaggtgatgacacggctttgtattactgt gcgtgcggccatatgcgggatggttatgatgtc IGA1 23 212 16 7 8 12 8 11 5 2 12 4 JY8QFUQ01ATX94 3646 productive (see comment) Homsap IGHV3-72*01 F 961 92.79 206/222 nt 92.34 205/222 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-3*01 F 3 8 10 11 8.10.11 [1.16.38.11] CITSFGGRSLAYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 52 of V-REGION: 1 nucleotides (from position 70 in the user submitted sequence), (cause frameshift) ctctggattcaccttaagtgatcactacatggactgggtccgccaggctccagggaaggggctggagtggttggccgtactaaaaacaaagctaacggttacactacacactacgccgcgtctgtgagaggcagattcattctttcaagagacgattcaaagaactcagtgtatctgcaaatgaacagcctgaaaatcgaggacacggccgtctattactgtattacttcttttggtggaagatccctggcgtactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggctgtcgtggaact NA IGG2 100 100 [287] c90>a,F30>L(+ - -)|c108>t|g170>a,R57>K(+ + +)|a184>g,S62>G(+ + -)|c192>t|g196>c,E66>H(- + -)|a198>c,E66>H(- + -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|c231>t,T77>I(- - -)|a232>c,I78>L(+ + +)|c234>t,I78>L(+ + +)|t243>c|c259>g,L87>V(+ - +)|c287>t,T96>I(- - -)|g303>c| c90>a,F30>L(+ - -)|c108>t| g170>a,R57>K(+ + +)|a184>g,S62>G(+ + -)|c192>t| g196>c,E66>H(- + -)|a198>c,E66>H(- + -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|c231>t,T77>I(- - -)|a232>c,I78>L(+ + +)|c234>t,I78>L(+ + +)|t243>c|c259>g,L87>V(+ - +)|c287>t,T96>I(- - -)|g303>c| g313>a,A105>I(+ - +)|c314>t,A105>I(+ - +)|g317>c,R106>T(- - -)|a318>t,R106>T(- - -)|g319>t|a320>c| 312 (320) 222 (230) 206 (208) 16 (22) 4 (6) 12 (16) 2 1 (2) 6 (7) 1 2 (3) 1 0 (1) 0 2 (3) 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 1 0 0 1 0 0 0 0 0 0 51 50 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 27 3 1 2 1 1 1 0 0 0 0 0 0 0 0 0 117 114 103 11 2 9 1 0 4 1 2 0 0 0 2 1 0 0 0 (8) 0 0 (2) 0 (6) 0 (2) 0 (4) 0 0 (1) 0 (1) 0 0 (1) 0 0 (1) 0 0 (1) 0 0 (1) 0 ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,165-168(FR2-CDR2)|agct,177-180(CDR2)|aact,253-256(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tgct,312-315(FR3)| ctct ggattcaccttaagtgatcactac atggactgggtccgccaggctccagggaaggggctggagtggttggccgt actaaaaacaaagctaacggttacactaca cactacgccgcgtctgtgagaggcagattcattctttcaagagacgattcaaagaactcagtgtatctgcaaatgaacagcctgaaaatcgaggacacggccgtctattactgt attacttcttttggtggaagatccctggcgtac ITSFGGRSLAY IGHV3-72 IGHD3-3 IGHJ4 ggattcaccttaagtgatcactac atggactgggtccgccaggctccagggaaggggctggagtggttggccgt actaaaaacaaagctaacggttacactaca cactacgccgcgtctgtgagaggcagattcattctttcaagagacgattcaaagaactcagtgtatctgcaaatgaacagcctgaaaatcgaggacacggccgtctattactgt attacttcttttggtggaagatccctggcgtac IGG2 16 218 10 6 7 11 3 5 2 2 9 3 JY8QFUQ01AU02Q 14501 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 857 87.78 194/221 nt NA Homsap IGHJ5*02 F (see comment) 138 74.51 38/51 nt Homsap IGHD1-1*01 F 1 10 7 14 10.7.14 [1.17.38.11] CARELGNTERGIVDPW in-frame + Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) tctctggtggctccatcagcgacactagttatcactgggcctggatgcgccagcccccagggaagggcctggagtggattgctaatgttcattatactggcagcgcccactacaacccgtccctcaagagtcgagtcaccatactagtagacacgtcccagaatcagttctccctgatgctgagttctgtgaccgccacagacacggctgtgtattactgtgcgagggaacttggtaatactgagaggggaatcgtcgacccctggggccagggaatcccggtcagcgtctccgcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [294] a100>g,S34>D(- - -)|g101>a,S34>D(- - -)|t102>c,S34>D(- - -)|g104>c,S35>T(+ - +)|c111>t|t112>c,Y38>H(+ - -)|g119>c,G40>A(- + -)|c126>g,I42>M(+ + -)|g147>c|g161>c,G54>A(- + -)|g162>t,G54>A(- + -)|g164>a,S55>N(- - -)|a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|g189>c|a193>g,T65>A(- - -)|t196>c,Y66>H(+ - -)|t235>c,S79>L(- - -)|c236>t,S79>L(- - -)|c237>a,S79>L(- - -)|a250>c,K84>Q(+ - -)|c255>t|a269>t,K90>M(- + -)|c276>t|g289>a,A97>T(- - -)| a100>g,S34>D(- - -)|g101>a,S34>D(- - -)|t102>c,S34>D(- - -)|g104>c,S35>T(+ - +)|c111>t|t112>c,Y38>H(+ - -)| g119>c,G40>A(- + -)|c126>g,I42>M(+ + -)|g147>c|g161>c,G54>A(- + -)|g162>t,G54>A(- + -)|g164>a,S55>N(- - -)| a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|g189>c|a193>g,T65>A(- - -)| t196>c,Y66>H(+ - -)|t235>c,S79>L(- - -)|c236>t,S79>L(- - -)|c237>a,S79>L(- - -)|a250>c,K84>Q(+ - -)|c255>t|a269>t,K90>M(- + -)|c276>t|g289>a,A97>T(- - -)| a318>g|c319>g| 317 (320) 226 (229) 199 (200) 27 (29) 5 (7) 22 3 (4) 3 5 5 1 1 1 0 6 1 (2) 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 1 5 1 1 1 2 0 0 0 0 1 0 0 0 51 51 45 6 1 5 0 1 0 0 0 0 0 0 3 1 1 0 30 21 15 6 1 5 2 0 1 1 0 0 0 0 2 0 0 0 117 114 105 9 2 7 0 1 3 2 1 1 1 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 0 (1) 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcgacactagttatcac tgggcctggatgcgccagcccccagggaagggcctggagtggattgctaat gttcattatactggcagcgcc cactacaacccgtccctcaagagtcgagtcaccatactagtagacacgtcccagaatcagttctccctgatgctgagttctgtgaccgccacagacacggctgtgtattactgt gcgagggaacttggtaatactgagaggggaatcgtcgacccc ARELGNTERGIVDP IGHV4-39 IGHD1-1 IGHJ5 ggtggctccatcagcgacactagttatcac tgggcctggatgcgccagcccccagggaagggcctggagtggattgctaat gttcattatactggcagcgcc cactacaacccgtccctcaagagtcgagtcaccatactagtagacacgtcccagaatcagttctccctgatgctgagttctgtgaccgccacagacacggctgtgtattactgt gcgagggaacttggtaatactgagaggggaatcgtcgacccc IGA1 27 216 16 11 8 17 8 10 3 2 12 10 JY8QFUQ01AU76E 10858 productive Homsap IGHV3-48*03 F 855 88.48 192/217 nt NA Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD3-3*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARDKGWSGYGMDVW in-frame + ctctggattcaccttcagttcttatgccatgaactgggtccgcctggttccaggcaaggggctggaatggctttcatttattggtaatactggtagtgtcatatactacgcagactctgtgaaggggcgattcaccatctccagagacaatgccaagaactcaatgtctctacaaatgagcagcctgagagccgaggacacggctctatattattgtgcgagagataaaggttggagcggatacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccggaccagccccaaggtcttcccgctgagcctcgacagcacccccccaaggatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccc NA IGA2 71 84 [288, 290] a106>t|g107>c|a113>c,E38>A(- - -)|a114>c,E38>A(- - -)|a131>t,Q44>L(- - -)|c134>t,A45>V(+ - +)|g141>c|g153>a|g157>c,V53>L(+ - +)|a164>t,Y55>F(- + -)|c165>t,Y55>F(- + -)|a169>g,S57>G(+ + -)|g173>a,S58>N(- - -)|g176>c,S59>T(+ - +)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)|c222>g|c246>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|a275>g,N92>S(- - -)|g301>c,V101>L(+ - +)|t303>a,V101>L(+ - +)|c309>t| a106>t|g107>c|a113>c,E38>A(- - -)|a114>c,E38>A(- - -)| a131>t,Q44>L(- - -)|c134>t,A45>V(+ - +)|g141>c|g153>a|g157>c,V53>L(+ - +)|a164>t,Y55>F(- + -)|c165>t,Y55>F(- + -)| a169>g,S57>G(+ + -)|g173>a,S58>N(- - -)|g176>c,S59>T(+ - +)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)| c222>g|c246>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|a275>g,N92>S(- - -)|g301>c,V101>L(+ - +)|t303>a,V101>L(+ - +)|c309>t| 320 225 200 25 8 17 3 3 5 0 3 1 3 1 5 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 0 0 0 2 0 1 0 1 0 0 0 51 51 44 7 2 5 0 1 2 0 0 0 2 0 2 0 0 0 30 24 19 5 0 5 2 1 1 0 0 0 0 0 1 0 0 0 117 114 105 9 4 5 1 1 2 0 1 1 0 1 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,113-114(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagttcttatgcc atgaactgggtccgcctggttccaggcaaggggctggaatggctttcattt attggtaatactggtagtgtcata tactacgcagactctgtgaaggggcgattcaccatctccagagacaatgccaagaactcaatgtctctacaaatgagcagcctgagagccgaggacacggctctatattattgt gcgagagataaaggttggagcggatacggtatggacgtc ARDKGWSGYGMDV IGHV3-48 IGHD3-3 IGHJ6 ggattcaccttcagttcttatgcc atgaactgggtccgcctggttccaggcaaggggctggaatggctttcattt attggtaatactggtagtgtcata tactacgcagactctgtgaaggggcgattcaccatctccagagacaatgccaagaactcaatgtctctacaaatgagcagcctgagagccgaggacacggctctatattattgt gcgagagataaaggttggagcggatacggtatggacgtc IGA2 25 213 11 14 8 15 3 10 6 2 10 7 JY8QFUQ01AUADL 22320 productive (see comment) Homsap IGHV3-30*07 F 847 87.67 192/219 nt 86.76 190/219 nt Homsap IGHJ5*02 F 147 76.47 39/51 nt Homsap IGHD3-22*01 F 2 8 8 12 8.8.12 [2.17.38.11] CASLGSYESSGPRW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'G' (1 nucleotides at position 60 to 60 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 74 of V-REGION: 'G' (1 nucleotides at position 129 to 129 in the user submitted sequence), (cause frameshift); gcctctggattcctcttcagtagttatgctatacactgggtccgccaggctccaggcaaGggggcttgagtgggtggcggttgtttcatatgatggaaataataaattttacgcagactctgtgaaggGgtcgattcagcctctccagagacaactccaagagcacggttgatctgcaaatggacaacttgagatccgaagacacggctgtatattattgtgcgagcctcggttcctatgagagcagtggtccccgatggggcccgggaaccctggtcaccgtctcctcagcatccccggaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [289] a85>c,T29>L(- - -)|c86>t,T29>L(- - -)|c108>t|g117>a,M39>I(+ + -)|a150>t|a162>g|a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|g188>a,S63>N(- - -)|a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|c210>t|c222>t|c230>g,T77>S(+ - +)|a232>c,I78>L(+ + +)|t246>c|a254>g,N85>S(- - -)|c259>g,L87>V(+ - +)|g261>t,L87>V(+ - +)|t262>g,Y88>D(- - -)|a274>g,N92>D(+ + -)|g278>a,S93>N(- - -)|c280>t|g286>t,A96>S(- + -)|g291>a|g303>a|c309>t| a85>c,T29>L(- - -)|c86>t,T29>L(- - -)|c108>t| g117>a,M39>I(+ + -)|a150>t|a162>g| a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|g188>a,S63>N(- - -)| a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|c210>t|c222>t|c230>g,T77>S(+ - +)|a232>c,I78>L(+ + +)|t246>c|a254>g,N85>S(- - -)|c259>g,L87>V(+ - +)|g261>t,L87>V(+ - +)|t262>g,Y88>D(- - -)|a274>g,N92>D(+ + -)|g278>a,S93>N(- - -)|c280>t|g286>t,A96>S(- + -)|g291>a|g303>a|c309>t| a318>c,R106>S(- - -)|g319>c|a320>t| 317 (320) 224 (227) 197 27 (30) 10 (12) 17 (18) 4 5 7 1 2 (3) 0 3 (4) 0 0 (1) 2 2 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 2 0 1 0 0 0 0 0 0 0 51 51 48 3 2 1 1 1 0 0 0 0 1 0 0 0 0 0 30 24 21 3 0 3 1 1 0 0 0 0 1 0 0 0 0 0 117 114 96 18 7 11 2 3 5 1 1 0 1 0 0 2 2 1 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 0 0 0 0 (1) 0 0 (1) 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcctcttcagtagttatgct atacactgggtccgccaggctccaggcaaggggcttgagtgggtggcggtt gtttcatatgatggaaataataaa ttttacgcagactctgtgaagggtcgattcagcctctccagagacaactccaagagcacggttgatctgcaaatggacaacttgagatccgaagacacggctgtatattattgt gcgagcctcggttcctatgagagcagtggtccccga ASLGSYESSGPR IGHV3-30 IGHD3-22 IGHJ5 ggattcctcttcagtagttatgct atacactgggtccgccaggctccaggcaaggggcttgagtgggtggcggtt gtttcatatgatggaaataataaa ttttacgcagactctgtgaagggtcgattcagcctctccagagacaactccaagagcacggttgatctgcaaatggacaacttgagatccgaagacacggctgtatattattgt gcgagcctcggttcctatgagagcagtggtccccga IGA1 27 213 17 10 12 16 5 11 9 1 12 5 JY8QFUQ01AUD41 15695 productive Homsap IGHV3-48*03 F 855 88.48 192/217 nt NA Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD3-3*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARDKGWSGYGMDVW in-frame + ctctggattcaccttcagttcttatgccatgaactgggtccgcctggttccaggcaaggggctggaatggctttcatttattggtaatactggtagtgtcatatactacgcagactctgtgaaggggcgattcaccatctccagagacaatgccaagaactcaatgtctctacaaatgagcagcctgagagccgaggacacggctctatattattgtgcgagagataaaggttggagcggatacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] a106>t|g107>c|a113>c,E38>A(- - -)|a114>c,E38>A(- - -)|a131>t,Q44>L(- - -)|c134>t,A45>V(+ - +)|g141>c|g153>a|g157>c,V53>L(+ - +)|a164>t,Y55>F(- + -)|c165>t,Y55>F(- + -)|a169>g,S57>G(+ + -)|g173>a,S58>N(- - -)|g176>c,S59>T(+ - +)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)|c222>g|c246>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|a275>g,N92>S(- - -)|g301>c,V101>L(+ - +)|t303>a,V101>L(+ - +)|c309>t| a106>t|g107>c|a113>c,E38>A(- - -)|a114>c,E38>A(- - -)| a131>t,Q44>L(- - -)|c134>t,A45>V(+ - +)|g141>c|g153>a|g157>c,V53>L(+ - +)|a164>t,Y55>F(- + -)|c165>t,Y55>F(- + -)| a169>g,S57>G(+ + -)|g173>a,S58>N(- - -)|g176>c,S59>T(+ - +)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)| c222>g|c246>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|a275>g,N92>S(- - -)|g301>c,V101>L(+ - +)|t303>a,V101>L(+ - +)|c309>t| 320 225 200 25 8 17 3 3 5 0 3 1 3 1 5 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 0 0 0 2 0 1 0 1 0 0 0 51 51 44 7 2 5 0 1 2 0 0 0 2 0 2 0 0 0 30 24 19 5 0 5 2 1 1 0 0 0 0 0 1 0 0 0 117 114 105 9 4 5 1 1 2 0 1 1 0 1 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,113-114(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagttcttatgcc atgaactgggtccgcctggttccaggcaaggggctggaatggctttcattt attggtaatactggtagtgtcata tactacgcagactctgtgaaggggcgattcaccatctccagagacaatgccaagaactcaatgtctctacaaatgagcagcctgagagccgaggacacggctctatattattgt gcgagagataaaggttggagcggatacggtatggacgtc ARDKGWSGYGMDV IGHV3-48 IGHD3-3 IGHJ6 ggattcaccttcagttcttatgcc atgaactgggtccgcctggttccaggcaaggggctggaatggctttcattt attggtaatactggtagtgtcata tactacgcagactctgtgaaggggcgattcaccatctccagagacaatgccaagaactcaatgtctctacaaatgagcagcctgagagccgaggacacggctctatattattgt gcgagagataaaggttggagcggatacggtatggacgtc IGA1 25 213 11 14 8 15 3 10 6 2 10 7 JY8QFUQ01AUF6C 10063 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 810 86.18 187/217 nt NA Homsap IGHJ6*02 F (see comment) 193 79.03 49/62 nt Homsap IGHD6-19*01 F 1 8 8 12 8.8.12 [1.17.38.11] CAGSGRDPYGINVW in-frame - Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ctctggattcacgtttagagactattggatgagttgggtccgccaggctccggggaagggactggagtgggtctcaaccattagtcctagtagtcagtacatatactatgcagactctgtggagggccgattcaccatctccagagtcgacgcccggagttcagtgtttctgcaaatgaacagcctgagagacgacgacacggctgtgtattactgtgcgggcagtggcagggatccgtacggtattaacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [284] c87>g|c90>t|t105>a,S35>R(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a112>t,S38>W(- - -)|c114>g,S38>W(- - -)|a119>g,N40>S(- - -)|c120>t,N40>S(- - -)|a138>g|g147>a|t163>a,S55>T(+ - +)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>g,S63>Q(- - -)|c201>t|a210>t|a214>g,K72>E(+ - -)|a242>t,D81>V(- - -)|a244>g,N82>D(+ + -)|a250>c,K84>R(+ + +)|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c287>a,A96>D(- - -)|g291>c,E97>D(+ - +)| c87>g|c90>t|t105>a,S35>R(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a112>t,S38>W(- - -)|c114>g,S38>W(- - -)| a119>g,N40>S(- - -)|c120>t,N40>S(- - -)|a138>g|g147>a|t163>a,S55>T(+ - +)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>g,S63>Q(- - -)| c201>t|a210>t|a214>g,K72>E(+ - -)|a242>t,D81>V(- - -)|a244>g,N82>D(+ + -)|a250>c,K84>R(+ + +)|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c287>a,A96>D(- - -)|g291>c,E97>D(+ - +)| a316>g,R106>G(- - -)|a318>c,R106>G(- - -)|g319>a|a320>g| 315 (320) 220 (225) 190 (191) 30 (34) 6 (8) 24 (26) 7 (9) 3 (4) 4 0 3 (4) 1 4 2 2 3 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 2 5 1 1 1 0 0 0 1 1 0 2 0 0 51 51 46 5 2 3 2 1 1 0 0 0 0 1 0 0 0 0 30 24 19 5 0 5 0 1 0 0 2 0 0 0 1 0 0 1 117 114 101 13 2 11 4 0 2 0 1 1 3 0 1 1 0 0 3 (8) 3 3 (4) 0 (4) 0 (2) 0 (2) 0 (2) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgtttagagactattgg atgagttgggtccgccaggctccggggaagggactggagtgggtctcaacc attagtcctagtagtcagtacata tactatgcagactctgtggagggccgattcaccatctccagagtcgacgcccggagttcagtgtttctgcaaatgaacagcctgagagacgacgacacggctgtgtattactgt gcgggcagtggcagggatccgtacggtattaacgtc AGSGRDPYGINV IGHV3-21 IGHD6-19 IGHJ6 ggattcacgtttagagactattgg atgagttgggtccgccaggctccggggaagggactggagtgggtctcaacc attagtcctagtagtcagtacata tactatgcagactctgtggagggccgattcaccatctccagagtcgacgcccggagttcagtgtttctgcaaatgaacagcctgagagacgacgacacggctgtgtattactgt gcgggcagtggcagggatccgtacggtattaacgtc IGA1 30 213 14 16 7 13 7 17 4 2 14 10 JY8QFUQ01AUH73 21224 productive Homsap IGHV3-9*01 F 914 91.28 199/218 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD6-19*01 F 1 8 8 14 8.8.14 [1.17.38.11] CVKDRGSSSKYYFDYW in-frame + cctctggattcacctttgatgattatgtcatgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagtattagttggggaagtgctcatatacactatgcggactctgtgaaggaccggttcaccatctccagagacaacgccatgaactccctttatttgcaaatggacagtctgagagctgacgactcggccttgtattactgtgtaaaagatcggggtagcagttccaagtattactttgactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctgtccagggcttcttccccca NA IGA1 100 100 [291] c113>t,A38>V(+ - +)|g163>a,G55>S(+ + -)|a175>g,N59>G(- - -)|a176>g,N59>G(- - -)|t177>a,N59>G(- - -)|g188>c,G63>A(- + -)|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|c192>t,S64>H(+ - -)|g196>c,G66>H(+ - -)|g197>a,G66>H(+ - -)|g221>a,G74>D(- - -)|a225>g|a251>t,K84>M(- + -)|g261>t|c265>t|a274>g,N92>D(+ + -)|g291>c,E97>D(+ - +)|a295>t,T99>S(+ - +)|c314>t,A105>V(+ - +)| c113>t,A38>V(+ - +)| g163>a,G55>S(+ + -)| a175>g,N59>G(- - -)|a176>g,N59>G(- - -)|t177>a,N59>G(- - -)|g188>c,G63>A(- + -)|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|c192>t,S64>H(+ - -)| g196>c,G66>H(+ - -)|g197>a,G66>H(+ - -)|g221>a,G74>D(- - -)|a225>g|a251>t,K84>M(- + -)|g261>t|c265>t|a274>g,N92>D(+ + -)|g291>c,E97>D(+ - +)|a295>t,T99>S(+ - +)| c314>t,A105>V(+ - +)|a322>c| 321 (322) 227 (228) 207 20 (21) 3 (4) 17 4 4 4 0 1 (2) 0 2 1 3 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 17 7 0 7 2 1 1 0 1 0 0 1 1 0 0 0 117 114 104 10 3 7 2 2 1 0 0 0 2 0 2 0 1 0 9 (10) 9 8 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttgatgattatgtc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt attagttggggaagtgctcatata cactatgcggactctgtgaaggaccggttcaccatctccagagacaacgccatgaactccctttatttgcaaatggacagtctgagagctgacgactcggccttgtattactgt gtaaaagatcggggtagcagttccaagtattactttgactat VKDRGSSSKYYFDY IGHV3-9 IGHD6-19 IGHJ4 ggattcacctttgatgattatgtc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt attagttggggaagtgctcatata cactatgcggactctgtgaaggaccggttcaccatctccagagacaacgccatgaactccctttatttgcaaatggacagtctgagagctgacgactcggccttgtattactgt gtaaaagatcggggtagcagttccaagtattactttgactat IGA1 19 213 11 8 7 11 4 8 3 0 8 8 JY8QFUQ01AUI48 19563 productive Homsap IGHV3-11*01 F 1008 96.31 209/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD5-18*01 F 3 8 8 18 8.8.18 [1.17.38.11] CVRRVWGYSYGSVSYYFEYW in-frame + ctctggattcaccttcagtgactactacatgaactggatccgccaggctccagggaaggggctggagtggctttcatacattagtggtagtggaactaccatatcctacgcagactctgtgaagggccgattcaccatctccagggacaacgccaggaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtgtattattgtgtgaggcgggtgtggggatatagttatggttccgtttcgtactactttgagtactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagcccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 79 95 [302, 303] g119>a,S40>N(- - -)|g157>c,V53>L(+ - +)|a172>g,S58>G(+ + -)|t186>a|g188>c,S63>T(+ - +)|a197>c,Y66>S(+ - -)|a251>g,K84>R(+ + +)|c309>t|c314>t,A105>V(+ - +)| g119>a,S40>N(- - -)|g157>c,V53>L(+ - +)| a172>g,S58>G(+ + -)|t186>a|g188>c,S63>T(+ - +)| a197>c,Y66>S(+ - -)|a251>g,K84>R(+ + +)|c309>t| c314>t,A105>V(+ - +)|a318>g|g319>c|a320>g| 317 (320) 222 (225) 213 9 (12) 2 (5) 7 2 (4) 1 2 0 1 0 0 1 2 (3) 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 30 24 21 3 1 2 1 0 0 0 0 0 0 1 1 0 0 0 117 114 111 3 1 2 1 0 1 0 1 0 0 0 0 0 0 0 5 (8) 5 4 1 (4) 0 (3) 1 0 (2) 0 1 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgactactac atgaactggatccgccaggctccagggaaggggctggagtggctttcatac attagtggtagtggaactaccata tcctacgcagactctgtgaagggccgattcaccatctccagggacaacgccaggaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtgtattattgt gtgaggcgggtgtggggatatagttatggttccgtttcgtactactttgagtac VRRVWGYSYGSVSYYFEY IGHV3-11 IGHD5-18 IGHJ4 ggattcaccttcagtgactactac atgaactggatccgccaggctccagggaaggggctggagtggctttcatac attagtggtagtggaactaccata tcctacgcagactctgtgaagggccgattcaccatctccagggacaacgccaggaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtgtattattgt gtgaggcgggtgtggggatatagttatggttccgtttcgtactactttgagtac IGA1 8 213 4 4 2 4 2 4 1 1 4 2 JY8QFUQ01AUJ6M 7828 productive (see comment) Homsap IGHV3-7*01 F 944 93.49 201/215 nt 93.02 200/215 nt Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD5-18*01 F 3 8 8 15 8.8.15 [1.16.38.11] CARVGYGYRGIYFFDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 41 of V-REGION: 1 nucleotides (from position 36 in the user submitted sequence), (cause frameshift) tctacattcacgtttagtcggtattggatgagctggtccgccaggctccagggaagggcctggagtgggtggccaacataaaggaagacggaagtgagagatattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgtgcgagagtgggctatggttaccgaggaatatacttctttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [292] g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|c87>g|a106>c,S36>R(- - -)|c108>g,S36>R(- - -)|g147>c|c172>g,Q58>E(+ + -)|t177>c|a194>g,K65>R(+ + +)|c198>t|c246>t|a258>t|a271>t,M91>L(+ + -)|c280>t| g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|c87>g|a106>c,S36>R(- - -)|c108>g,S36>R(- - -)| g147>c| c172>g,Q58>E(+ + -)|t177>c|a194>g,K65>R(+ + +)| c198>t|c246>t|a258>t|a271>t,M91>L(+ + -)|c280>t| a320>t| 319 (320) 222 (223) 208 14 (15) 7 (8) 7 1 1 3 1 1 0 2 (3) 0 2 3 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 0 0 1 0 0 0 1 2 0 0 51 50 49 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 21 3 1 2 1 0 0 1 0 0 0 0 0 1 0 0 117 114 109 5 4 1 0 0 3 0 0 0 2 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct acattcacgtttagtcggtattgg atgagctggtccgccaggctccagggaagggcctggagtgggtggccaac ataaaggaagacggaagtgagaga tattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgt gcgagagtgggctatggttaccgaggaatatacttctttgactac ARVGYGYRGIYFFDY IGHV3-7 IGHD5-18 IGHJ4 acattcacgtttagtcggtattgg atgagctggtccgccaggctccagggaagggcctggagtgggtggccaac ataaaggaagacggaagtgagaga tattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgt gcgagagtgggctatggttaccgaggaatatacttctttgactac IGG2 14 212 6 8 4 9 2 5 5 2 1 6 JY8QFUQ01AURES 16207 productive (see comment) Homsap IGHV1-2*02 F 835 88.26 188/213 nt 87.32 186/213 nt Homsap IGHJ4*02 F (see comment) 204 91.67 44/48 nt Homsap IGHD3-22*01 F 2 8 8 16 8.8.16 [0.17.37.11] CAKHPLRSTSGHSYLDSW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT at codon 36 of V-REGION: 'A' (1 nucleotides at position 18 to 18 in the user submitted sequence), (cause frameshift); in FR3-IMGT, from codon 72 of V-REGION: 1 nucleotides (from position 120 in the user submitted sequence), (cause frameshift) tggatacatattcaccgAccttttatatacactgggtgcgacaggcccctggacaagggcttgagtggatgggatccatcaatcccaacagtggtgtcacaacctacgaagagaagtttcgggcagggtcaccatgaccagggacacgtccatcactacagcctacatggaactgacaagccttacatcagacgacacggccgtatattactgtgcgaaacatcctcttcgatcaacaagtggacattcctacttagattcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [293] c86>t,T29>I(- - -)|c87>a,T29>I(- - -)|g107>c,G36>A(- + -)|a110>t,Y37>F(- + -)|c111>t,Y37>F(- + -)|g117>a,M39>I(+ + -)|g164>c,W55>S(- - -)|g165>c,W55>S(- - -)|c171>t|t174>c|g191>t,G64>V(- - -)|a197>c,N66>T(- + -)|t201>c|c203>a,A68>E(- - -)|c205>g,Q69>E(+ + -)|g254>c,S85>T(+ - +)|c255>t,S85>T(+ - +)|g270>a|g275>c,S92>T(+ - +)|c276>a,S92>T(+ - +)|g279>c,R93>S(- - -)|g282>t|g284>c,R95>T(- - -)|t288>a|g303>a| c86>t,T29>I(- - -)|c87>a,T29>I(- - -)|g107>c,G36>A(- + -)|a110>t,Y37>F(- + -)|c111>t,Y37>F(- + -)| g117>a,M39>I(+ + -)|g164>c,W55>S(- - -)|g165>c,W55>S(- - -)| c171>t|t174>c|g191>t,G64>V(- - -)| a197>c,N66>T(- + -)|t201>c|c203>a,A68>E(- - -)|c205>g,Q69>E(+ + -)|g254>c,S85>T(+ - +)|c255>t,S85>T(+ - +)|g270>a|g275>c,S92>T(+ - +)|c276>a,S92>T(+ - +)|g279>c,R93>S(- - -)|g282>t|g284>c,R95>T(- - -)|t288>a|g303>a| g317>a,R106>K(+ + +)|g319>c| 316 (320) 217 (221) 192 (194) 25 (27) 7 (8) 18 (19) 0 3 (4) 4 2 1 3 1 1 7 (8) 1 2 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 0 2 0 0 1 1 0 1 0 0 0 51 51 48 3 0 3 0 1 0 0 0 0 0 0 2 0 0 0 30 24 21 3 2 1 0 0 1 1 0 0 0 0 0 0 1 0 117 113 99 14 5 9 0 2 1 1 1 2 0 1 4 1 1 0 4 (8) 4 4 (6) 0 (2) 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 (1) 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatacatattcaccgccttttat atacactgggtgcgacaggcccctggacaagggcttgagtggatgggatcc atcaatcccaacagtggtgtcaca acctacgaagagaagtttcgggcagggtcaccatgaccagggacacgtccatcactacagcctacatggaactgacaagccttacatcagacgacacggccgtatattactgt gcgaaacatcctcttcgatcaacaagtggacattcctacttagattcc AKHPLRSTSGHSYLDS IGHV1-2 IGHD3-22 IGHJ4 ggatacatattcaccgccttttat atacactgggtgcgacaggcccctggacaagggcttgagtggatgggatcc atcaatcccaacagtggtgtcaca acctacgaagagaagtttcgggcagggtcaccatgaccagggacacgtccatcactacagcctacatggaactgacaagccttacatcagacgacacggccgtatattactgt gcgaaacatcctcttcgatcaacaagtggacattcctacttagattcc IGA1 25 212 9 16 7 20 2 5 5 2 12 6 JY8QFUQ01AUU0J 6682 productive Homsap IGHV3-7*01 F 945 93.09 202/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-2*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSAFFSTGHW in-frame + ctctagattcacctttaggacatattggatgagttgggtccgccaagctccagggaaggggctggagtgggtggccaacataaaggaagatggaagtgagatacactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtttctccaaatgaacagcttgagagtcgatgacacggctgtgtattactgtgcgatcagtagtgccttcttctccacgggccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [281] g79>a,G27>R(- - -)|t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|c120>t|g132>a|c172>g,Q58>E(+ + -)|a194>t,K65>I(- + -)|t196>c,Y66>H(+ - -)|t256>a,S86>T(+ - +)|a263>t,Y88>F(- + -)|g267>c|c280>t|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)| g79>a,G27>R(- - -)|t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)| c120>t|g132>a| c172>g,Q58>E(+ + -)|a194>t,K65>I(- + -)| t196>c,Y66>H(+ - -)|t256>a,S86>T(+ - +)|a263>t,Y88>F(- + -)|g267>c|c280>t|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)| g317>t,R106>I(- + -)|a318>c,R106>I(- + -)|g319>a|a320>g| 316 (320) 221 (225) 206 15 (19) 4 (6) 11 (13) 0 (1) 2 (3) 3 1 0 (1) 1 2 1 2 1 1 (2) 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 0 0 0 1 0 0 1 0 0 1 51 51 49 2 2 0 0 1 1 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 117 114 107 7 2 5 0 0 2 1 0 0 1 1 1 0 1 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct agattcacctttaggacatattgg atgagttgggtccgccaagctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagata cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtttctccaaatgaacagcttgagagtcgatgacacggctgtgtattactgt gcgatcagtagtgccttcttctccacgggccac AISSAFFSTGH IGHV3-7 IGHD2-2 IGHJ4 agattcacctttaggacatattgg atgagttgggtccgccaagctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagata cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtttctccaaatgaacagcttgagagtcgatgacacggctgtgtattactgt gcgatcagtagtgccttcttctccacgggccac IGA2 15 213 6 9 5 10 1 5 4 0 5 6 JY8QFUQ01AUUPD 10094 productive (see comment) Homsap IGHV3-11*01 F 934 93.43 199/213 nt 92.96 198/213 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-15*01 F 2 8 7 18 8.7.18 [1.17.38.11] CARDGPPYLYGSHGNSFDQW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 63 of V-REGION: 3 nucleotides (from position 94 in the user submitted sequence), (do not cause frameshift) tctggattcagcttcagtgactactacatgagttgggtccgccaggctccagggaagggactggagtgggtttcatgcatcactactagtggtaccacattctacacggactctgtgaggggccgattcaccatgtccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtgtattactgtgcgagagatggcccaccttatttatatggaagtcatggtaattcctttgaccagtggggccagggaaccctagtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [299] c86>g,T29>S(+ - +)|c120>t|a124>g,I42>V(+ - +)|g147>a|a164>g,Y55>C(- - -)|t168>c|g170>c,S57>T(+ - +)|g173>c,S58>T(+ - +)|t194>c,I65>T(- - -)|a197>t,Y66>F(- + -)|g202>a,A68>T(- - -)|a204>g,A68>T(- - -)|a215>g,K72>R(+ + +)|c234>g,I78>M(+ + -)| c86>g,T29>S(+ - +)| c120>t|a124>g,I42>V(+ - +)|g147>a|a164>g,Y55>C(- - -)| t168>c|g170>c,S57>T(+ - +)|g173>c,S58>T(+ - +)|t194>c,I65>T(- - -)| a197>t,Y66>F(- + -)|g202>a,A68>T(- - -)|a204>g,A68>T(- - -)|a215>g,K72>R(+ + +)|c234>g,I78>M(+ + -)| 320 221 207 14 3 11 4 2 1 2 0 0 1 0 2 2 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 47 4 2 2 2 1 1 0 0 0 0 0 0 0 0 0 30 21 17 4 1 3 0 0 0 2 0 0 0 0 2 0 0 0 117 114 109 5 0 5 2 1 0 0 0 0 1 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcagcttcagtgactactac atgagttgggtccgccaggctccagggaagggactggagtgggtttcatgc atcactactagtggtaccaca ttctacacggactctgtgaggggccgattcaccatgtccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtgtattactgt gcgagagatggcccaccttatttatatggaagtcatggtaattcctttgaccag ARDGPPYLYGSHGNSFDQ IGHV3-11 IGHD2-15 IGHJ4 ggattcagcttcagtgactactac atgagttgggtccgccaggctccagggaagggactggagtgggtttcatgc atcactactagtggtaccaca ttctacacggactctgtgaggggccgattcaccatgtccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtgtattactgt gcgagagatggcccaccttatttatatggaagtcatggtaattcctttgaccag IGG1 14 210 9 5 3 7 6 7 2 1 7 4 JY8QFUQ01AUVXR 7872 productive Homsap IGHV3-48*02 F 918 91.71 199/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [1.17.38.11] CARDVPGPNDNYFDYW in-frame + ctctggattcagcttcagagactatggcatggcctgggtccgccaggctccagggaaggggctggagtgggtctcatacattggtaggattattagtgacatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagggacgacgacacggctgtctatttctgtgcgagagatgtcccgggccccaatgacaactactttgactactggggccagggaaccctgcttaccgtcgcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggctccagggcttcttccccca NA IGA2 100 100 [290] c86>g,T29>S(+ - +)|t105>a,S35>R(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a112>g,S38>G(+ + -)|a118>g,N40>A(- - -)|a119>c,N40>A(- - -)|t159>c|a169>g,S57>G(+ + -)|t174>g,S58>R(- - -)|g176>t,S59>I(- - -)|g185>t,S62>I(- - -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|a285>g|g291>c,E97>D(+ - +)|g303>c|a308>t,Y103>F(- + -)| c86>g,T29>S(+ - +)|t105>a,S35>R(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a112>g,S38>G(+ + -)| a118>g,N40>A(- - -)|a119>c,N40>A(- - -)|t159>c| a169>g,S57>G(+ + -)|t174>g,S58>R(- - -)|g176>t,S59>I(- - -)|g185>t,S62>I(- - -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)| a285>g|g291>c,E97>D(+ - +)|g303>c|a308>t,Y103>F(- + -)| 320 225 207 18 3 15 6 1 0 1 1 1 1 1 2 1 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 2 1 0 0 0 0 0 1 0 1 0 0 51 51 48 3 1 2 1 0 0 1 1 0 0 0 0 0 0 0 30 24 18 6 0 6 2 0 0 0 0 1 0 0 0 0 2 1 117 114 110 4 2 2 1 0 0 0 0 0 1 0 2 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ctct ggattcagcttcagagactatggc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcatac attggtaggattattagtgacata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagggacgacgacacggctgtctatttctgt gcgagagatgtcccgggccccaatgacaactactttgactac ARDVPGPNDNYFDY IGHV3-48 IGHD5-24 IGHJ4 ggattcagcttcagagactatggc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcatac attggtaggattattagtgacata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagggacgacgacacggctgtctatttctgt gcgagagatgtcccgggccccaatgacaactactttgactac IGA2 18 213 8 10 1 7 7 11 3 0 4 11 JY8QFUQ01AUYQV 12762 productive (see comment) Homsap IGHV3-66*02 F 876 90.19 193/214 nt 89.72 192/214 nt Homsap IGHJ5*02 F 192 86.27 44/51 nt Homsap IGHD3-16*01 F 2 8 7 13 8.7.13 [1.17.38.11] CAALHDPPWGWLDPW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 56 of V-REGION: 'A' (1 nucleotides at position 81 to 81 in the user submitted sequence), (cause frameshift); ctctggattcagcgtcaacaacaactacatgaactgggtccgcctggctccagggaagggtctggagtgggtctcagttaAtttatagaggtggtaatacattctacacagactccgtgaagggccggttcaccatctccagagacatttccaagaacactttgtatcttcaaatgaacagtctgacaactgaggacacggctgtgtactattgtgcggccctccacgaccctccttggggttggctcgacccctggggccggggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [285] c86>g,T29>S(+ - +)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)|g119>a,S40>N(- - -)|a131>t,Q44>L(- - -)|g147>t|c174>a,S58>R(- - -)|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)|a197>t,Y66>F(- + -)|g202>a,A68>T(- - -)|a225>g|a245>t,N82>I(- - -)|g258>t|c259>t|c279>t|g284>c,R95>T(- - -)|g286>a,A96>T(- - -)|t306>c|c309>t| c86>g,T29>S(+ - +)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)| g119>a,S40>N(- - -)|a131>t,Q44>L(- - -)|g147>t| c174>a,S58>R(- - -)|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)| a197>t,Y66>F(- + -)|g202>a,A68>T(- - -)|a225>g|a245>t,N82>I(- - -)|g258>t|c259>t|c279>t|g284>c,R95>T(- - -)|g286>a,A96>T(- - -)|t306>c|c309>t| a316>g,R106>A(- - -)|g317>c,R106>A(- - -)|a318>c,R106>A(- - -)| 315 (318) 217 (220) 196 21 (24) 7 14 (17) 1 (2) 6 4 2 0 (1) 1 3 0 1 (2) 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 2 0 1 0 0 0 0 0 1 0 0 51 51 48 3 1 2 0 1 0 0 0 0 1 0 0 0 1 0 30 21 18 3 0 3 0 1 1 0 0 1 0 0 0 0 0 0 117 114 103 11 6 5 1 2 3 1 0 0 2 0 1 0 1 0 3 (6) 3 3 0 (3) 0 0 (3) 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagcgtcaacaacaactac atgaactgggtccgcctggctccagggaagggtctggagtgggtctcagtt atttatagaggtggtaataca ttctacacagactccgtgaagggccggttcaccatctccagagacatttccaagaacactttgtatcttcaaatgaacagtctgacaactgaggacacggctgtgtactattgt gcggccctccacgaccctccttggggttggctcgacccc AALHDPPWGWLDP IGHV3-66 IGHD3-16 IGHJ5 ggattcagcgtcaacaacaactac atgaactgggtccgcctggctccagggaagggtctggagtgggtctcagtt atttatagaggtggtaataca ttctacacagactccgtgaagggccggttcaccatctccagagacatttccaagaacactttgtatcttcaaatgaacagtctgacaactgaggacacggctgtgtactattgt gcggccctccacgaccctccttggggttggctcgacccc IGA1 21 210 13 8 10 15 3 6 7 0 7 7 JY8QFUQ01AV09F 3071 productive Homsap IGHV4-30-4*03 F, or Homsap IGHV4-31*06 F (see comment) 740 81.82 180/220 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-15*01 F 3 10 7 15 10.7.15 [1.17.38.11] CARVWVAGSRDENFDSW in-frame + Low V-REGION identity (81,82% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tctcgggtgcttccatgacgagtactaatttctactggagttgggtccgccaacgctcagggaagggcctagagtggcttggatacatctatcacagtgggggcacctattacaacccgtctctcaagagtcgacttgccctgtcattagacgcgtctaataatgttttctccctgaaattggcctctgtaaccgccgcggacacggccgtatattactgtgcgagagtgtgggtggctggctctcgagacgagaactttgactcctggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] t78>g|g83>c,G28>A(- + -)|c84>t,G28>A(- + -)|c90>g,I30>M(+ + -)|g92>c,S31>T(+ - +)|c93>g,S31>T(+ - +)|g103>a,G35>T(+ - -)|g104>c,G35>T(+ - -)|g106>a,D36>N(+ + -)|a110>t,Y37>F(- + -)|a124>g,I42>V(+ - +)|g132>a|c134>g,P45>R(- - -)|c136>t,P46>S(+ - -)|g150>a|a157>c,I53>L(+ + +)|g162>a|t172>c,Y58>H(+ - -)|a190>g,S64>G(+ + -)|c198>t|c210>t|g226>c,V76>L(+ - +)|a229>g,T77>A(- - -)|a232>c,I78>L(+ + +)|a234>g,I78>L(+ + +)|g238>t,V80>L(+ - +)|a244>g,T82>A(- - -)|c249>t|g252>t,K84>N(+ - -)|c255>t|c256>g,Q86>V(- + -)|a257>t,Q86>V(- + -)|g258>t,Q86>V(- + -)|g270>a|c271>t|a274>g,S92>A(- + -)|g275>c,S92>A(- + -)|g282>a|t285>c|g303>a| t78>g| g83>c,G28>A(- + -)|c84>t,G28>A(- + -)|c90>g,I30>M(+ + -)|g92>c,S31>T(+ - +)|c93>g,S31>T(+ - +)|g103>a,G35>T(+ - -)|g104>c,G35>T(+ - -)|g106>a,D36>N(+ + -)|a110>t,Y37>F(- + -)| a124>g,I42>V(+ - +)|g132>a|c134>g,P45>R(- - -)|c136>t,P46>S(+ - -)|g150>a|a157>c,I53>L(+ + +)|g162>a| t172>c,Y58>H(+ - -)|a190>g,S64>G(+ + -)| c198>t|c210>t|g226>c,V76>L(+ - +)|a229>g,T77>A(- - -)|a232>c,I78>L(+ + +)|a234>g,I78>L(+ + +)|g238>t,V80>L(+ - +)|a244>g,T82>A(- - -)|c249>t|g252>t,K84>N(+ - -)|c255>t|c256>g,Q86>V(- + -)|a257>t,Q86>V(- + -)|g258>t,Q86>V(- + -)|g270>a|c271>t|a274>g,S92>A(- + -)|g275>c,S92>A(- + -)|g282>a|t285>c|g303>a| 311 220 180 40 13 27 6 8 7 2 2 0 2 0 5 4 3 1 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 36 30 21 9 0 9 0 2 1 0 0 0 1 0 3 2 0 0 51 51 44 7 3 4 1 3 1 0 1 0 0 0 0 1 0 0 30 21 19 2 0 2 1 0 0 1 0 0 0 0 0 0 0 0 116 114 92 21 9 12 4 3 5 1 1 0 1 0 2 1 3 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,228-229(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,227-228(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agtt,118-121(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agtt,225-228(FR3)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|tacc,228-231(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tgcc,285-288(FR3)|tact,307-310(FR3)| tctcg ggtgcttccatgacgagtactaatttctac tggagttgggtccgccaacgctcagggaagggcctagagtggcttggatac atctatcacagtgggggcacc tattacaacccgtctctcaagagtcgacttgccctgtcattagacgcgtctaataatgttttctccctgaaattggcctctgtaaccgccgcggacacggccgtatattactgt gcgagagtgtgggtggctggctctcgagacgagaactttgactcc ARVWVAGSRDENFDS IGHV4-30-4 IGHD2-15 IGHJ4 ggtgcttccatgacgagtactaatttctac tggagttgggtccgccaacgctcagggaagggcctagagtggcttggatac atctatcacagtgggggcacc tattacaacccgtctctcaagagtcgacttgccctgtcattagacgcgtctaataatgttttctccctgaaattggcctctgtaaccgccgcggacacggccgtatattactgt gcgagagtgtgggtggctggctctcgagacgagaactttgactcc IGA1 39 216 23 16 15 27 8 12 12 0 16 11 JY8QFUQ01AV2V7 18313 productive Homsap IGHV3-72*01 F 930 91.03 203/223 nt NA Homsap IGHJ6*02 F 184 77.42 48/62 nt Homsap IGHD3-3*01 F 2 8 10 14 8.10.14 [1.17.38.11] CLRGFSSHYRGGMDVW in-frame - ctctggactcacgttcagtgaccactacatggactgggtccgccagactccagggaggggactggagtgggttggccgtattagagacaaagctcgcaggtacaccacagaatacgccgcgtctgtgaaaggcagattcaccattttaggagatgatttaaagaattcactgtatctacaaatgaacaacctgagaaccgacgactcggccgtgtattactgtctcagaggattttcgagccattatcggggcggtatggacgtctggggccaagggaccacggtcaccgtgtccgcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [296] t82>c,F28>L(+ - -)|c87>g|g133>a,A45>T(- - -)|a143>g,K48>R(+ + +)|g147>a|c167>t,T56>I(- - -)|a172>g,N58>D(+ + -)|a181>c,N61>R(+ - -)|a182>g,N61>R(+ - -)|t186>g,S62>R(- - -)|c234>t|c236>t,S79>L(- - -)|a238>g,R80>G(- - -)|c248>t,S83>L(- - -)|c255>t|g267>a|g278>a,S93>N(- - -)|a284>g,K95>R(+ + +)|g291>c,E97>D(+ - +)|a295>t,T99>S(+ - +)| t82>c,F28>L(+ - -)|c87>g| g133>a,A45>T(- - -)|a143>g,K48>R(+ + +)|g147>a| c167>t,T56>I(- - -)|a172>g,N58>D(+ + -)|a181>c,N61>R(+ - -)|a182>g,N61>R(+ - -)|t186>g,S62>R(- - -)| c234>t|c236>t,S79>L(- - -)|a238>g,R80>G(- - -)|c248>t,S83>L(- - -)|c255>t|g267>a|g278>a,S93>N(- - -)|a284>g,K95>R(+ + +)|g291>c,E97>D(+ - +)|a295>t,T99>S(+ - +)| g313>c,A105>L(+ - +)|c314>t,A105>L(+ - +)|t315>c,A105>L(+ - +)|a320>g| 319 (320) 230 (231) 207 23 (24) 5 (6) 18 5 (6) 4 6 2 1 0 1 0 2 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 0 1 0 0 0 0 0 1 0 0 51 51 48 3 1 2 1 2 0 0 0 0 0 0 0 0 0 0 30 30 25 5 0 5 2 0 1 0 1 0 0 0 0 0 0 1 117 114 104 10 3 7 2 2 4 0 0 0 1 0 1 0 0 0 7 (8) 7 4 3 (4) 0 (1) 3 0 (1) 0 1 1 0 0 0 0 1 0 0 0 ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,165-168(FR2-CDR2)|agct,177-180(CDR2)|aact,253-256(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tgct,312-315(FR3)| ctct ggactcacgttcagtgaccactac atggactgggtccgccagactccagggaggggactggagtgggttggccgt attagagacaaagctcgcaggtacaccaca gaatacgccgcgtctgtgaaaggcagattcaccattttaggagatgatttaaagaattcactgtatctacaaatgaacaacctgagaaccgacgactcggccgtgtattactgt ctcagaggattttcgagccattatcggggcggtatggacgtc LRGFSSHYRGGMDV IGHV3-72 IGHD3-3 IGHJ6 ggactcacgttcagtgaccactac atggactgggtccgccagactccagggaggggactggagtgggttggccgt attagagacaaagctcgcaggtacaccaca gaatacgccgcgtctgtgaaaggcagattcaccattttaggagatgatttaaagaattcactgtatctacaaatgaacaacctgagaaccgacgactcggccgtgtattactgt ctcagaggattttcgagccattatcggggcggtatggacgtc IGA2 20 219 15 5 9 11 6 9 4 1 9 6 JY8QFUQ01AV3HD 4633 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD5-12*01 F 3 8 8 10 8.8.10 [1.17.38.11] CARDLYGADDLW in-frame + ctctggattcacctttagtagatattggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtattaatgaagatgggaggaccacaacctacgcggactccgtgaatggccgattcctcatctccagagacaacgccaagaatacgttgtatctgcagatgagcagtctgagagtcgaggacacggccatgtattattgtgcaagagatctatatggtgccgatgacctctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 71 100 [278] c90>t|c108>a,S36>R(- - -)|c111>t|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t189>g,S63>R(- - -)|g191>c,S64>T(+ - +)|g197>c,S66>T(+ - +)|g216>t,K72>N(+ - -)|a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|c255>t|c259>t|a270>g|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|t300>c|g301>a,V101>M(+ - -)|c309>t| c90>t|c108>a,S36>R(- - -)|c111>t| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t189>g,S63>R(- - -)|g191>c,S64>T(+ - +)| g197>c,S66>T(+ - +)|g216>t,K72>N(+ - -)|a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|c255>t|c259>t|a270>g|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|t300>c|g301>a,V101>M(+ - -)|c309>t| 320 225 205 20 7 13 3 2 7 1 1 1 0 1 2 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 2 0 0 1 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 19 5 0 5 1 1 0 0 0 0 0 1 1 0 0 1 117 114 102 12 5 7 2 1 5 1 1 0 0 0 1 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtagatattgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attaatgaagatgggaggaccaca acctacgcggactccgtgaatggccgattcctcatctccagagacaacgccaagaatacgttgtatctgcagatgagcagtctgagagtcgaggacacggccatgtattattgt gcaagagatctatatggtgccgatgacctc ARDLYGADDL IGHV3-74 IGHD5-12 IGHJ4 ggattcacctttagtagatattgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attaatgaagatgggaggaccaca acctacgcggactccgtgaatggccgattcctcatctccagagacaacgccaagaatacgttgtatctgcagatgagcagtctgagagtcgaggacacggccatgtattattgt gcaagagatctatatggtgccgatgacctc IGA1 20 213 13 7 9 13 4 7 5 2 7 6 JY8QFUQ01AV3VG 10844 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 868 89.35 193/216 nt 88.89 192/216 nt Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD3-22*01 F 3 7 8 15 7.8.15 [1.17.38.11] CARRSNPNAMMAAFDIW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 36 of V-REGION: 1 nucleotides (from position 20 in the user submitted sequence), (cause frameshift) ttctggattcagtcctaccgattttggatcggctgggttcgccagctgcccggcaaaggcctggagtggatgggcctcatttatcctggtgactctgagaccagattcaacccgtccttccaaggccaggtcaccatctcagccaacaagtccataaataccgcctacctacagtggagcagcctgaaggcctcggacactgccgtgtattactgtgcgaggcgatccaaccctaatgctatgatggcggcgtttgatatctggggccaagggacaatggtcaccgtttcttcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaa NA IGG1 79 94 [293] a83>t,Y28>F(- + -)|c87>t|t88>c,F30>P(- - -)|t89>c,F30>P(- - -)|c108>a|a110>t,Y37>F(- + -)|c111>t,Y37>F(- + -)|g126>t|a133>c,M45>L(+ + -)|g141>c|g162>c|a163>c,I55>L(+ + +)|c168>t|t192>g,D64>E(+ - +)|a200>t,Y67>F(- + -)|g203>a,S68>N(- - -)|g241>a,D81>N(+ + -)|c252>a|g254>a,S85>N(- - -)|c255>t,S85>N(- - -)|g267>a|c297>t|a301>g,M101>V(+ - -)| a83>t,Y28>F(- + -)|c87>t|t88>c,F30>P(- - -)|t89>c,F30>P(- - -)|c108>a|a110>t,Y37>F(- + -)|c111>t,Y37>F(- + -)| g126>t|a133>c,M45>L(+ + -)|g141>c|g162>c|a163>c,I55>L(+ + +)| c168>t|t192>g,D64>E(+ - +)| a200>t,Y67>F(- + -)|g203>a,S68>N(- - -)|g241>a,D81>N(+ + -)|c252>a|g254>a,S85>N(- - -)|c255>t,S85>N(- - -)|g267>a|c297>t|a301>g,M101>V(+ - -)| a318>g|a320>g| 317 (320) 221 (224) 198 (199) 23 (25) 9 (11) 14 1 (3) 4 5 2 2 2 3 0 2 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 16 7 2 5 0 0 2 2 0 1 2 0 0 0 0 0 51 51 46 5 3 2 0 0 0 0 2 0 0 0 2 0 1 0 30 24 22 2 1 1 0 0 1 0 0 0 0 0 0 0 0 1 117 114 105 9 3 6 1 4 2 0 0 1 1 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ttct ggattcagtcctaccgattttgg atcggctgggttcgccagctgcccggcaaaggcctggagtggatgggcctc atttatcctggtgactctgagacc agattcaacccgtccttccaaggccaggtcaccatctcagccaacaagtccataaataccgcctacctacagtggagcagcctgaaggcctcggacactgccgtgtattactgt gcgaggcgatccaaccctaatgctatgatggcggcgtttgatatc ARRSNPNAMMAAFDI IGHV5-51 IGHD3-22 IGHJ3 ggattcagtcctaccgattttgg atcggctgggttcgccagctgcccggcaaaggcctggagtggatgggcctc atttatcctggtgactctgagacc agattcaacccgtccttccaaggccaggtcaccatctcagccaacaagtccataaataccgcctacctacagtggagcagcctgaaggcctcggacactgccgtgtattactgt gcgaggcgatccaaccctaatgctatgatggcggcgtttgatatc IGG1 23 212 12 11 9 14 3 9 6 3 8 6 JY8QFUQ01AV7LU 12037 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 878 89.45 195/218 nt NA Homsap IGHJ6*02 F 256 90.32 56/62 nt Homsap IGHD1-26*01 F 3 8 8 21 8.8.21 [1.17.38.11] CAKYVEGWSYTGGYYNSYAMDVW in-frame - cttctggattcgcctttagccgttatgccatgacttgggtccgccagactccagggagggggctggagtgggtctcaggtctcagtggtagtggtgatagcacatactactcaaactccgtgaagggccggttcatcatcttcagagacaattccaagaacacgctgtatctgcaaataaacgccctgagagacgaagacacggccgtttactactgtgcgaaatatgtggaggggtggtcatatacggggggctactacaactcctacgctatggacgtctggggccaagggaccgcggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [313] c75>t|a85>g,T29>A(- - -)|a106>c,S36>R(- - -)|c108>t,S36>R(- - -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g133>a,A45>T(- - -)|a143>g,K48>R(+ + +)|c164>g,A55>G(- + -)|a166>c,I56>L(+ + +)|t168>c,I56>L(+ + +)|g188>a,G63>D(- - -)|g202>t,A68>S(- + -)|g205>a,D69>N(+ + -)|c230>t,T77>I(- - -)|c236>t,S79>F(- - -)|g273>a,M91>I(+ + -)|a277>g,S93>A(- + -)|g278>c,S93>A(- + -)|c287>a,A96>D(- - -)|g291>a|a303>t|t306>c| c75>t| a85>g,T29>A(- - -)|a106>c,S36>R(- - -)|c108>t,S36>R(- - -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g133>a,A45>T(- - -)|a143>g,K48>R(+ + +)|c164>g,A55>G(- + -)| a166>c,I56>L(+ + +)|t168>c,I56>L(+ + +)|g188>a,G63>D(- - -)| g202>t,A68>S(- + -)|g205>a,D69>N(+ + -)|c230>t,T77>I(- - -)|c236>t,S79>F(- - -)|g273>a,M91>I(+ + -)|a277>g,S93>A(- + -)|g278>c,S93>A(- + -)|c287>a,A96>D(- - -)|g291>a|a303>t|t306>c| g319>t| 318 (320) 224 (226) 201 (202) 23 (24) 4 (5) 19 3 5 5 2 2 1 1 0 2 1 1 (2) 0 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 0 1 0 1 0 0 0 0 0 0 0 51 51 46 5 0 5 1 1 1 0 0 0 0 0 1 1 0 0 30 24 21 3 0 3 0 1 0 1 1 0 0 0 0 0 0 0 117 114 103 11 3 8 1 3 2 1 0 1 1 0 1 0 1 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cttct ggattcgcctttagccgttatgcc atgacttgggtccgccagactccagggagggggctggagtgggtctcaggt ctcagtggtagtggtgatagcaca tactactcaaactccgtgaagggccggttcatcatcttcagagacaattccaagaacacgctgtatctgcaaataaacgccctgagagacgaagacacggccgtttactactgt gcgaaatatgtggaggggtggtcatatacggggggctactacaactcctacgctatggacgtc AKYVEGWSYTGGYYNSYAMDV IGHV3-23 IGHD1-26 IGHJ6 ggattcgcctttagccgttatgcc atgacttgggtccgccagactccagggagggggctggagtgggtctcaggt ctcagtggtagtggtgatagcaca tactactcaaactccgtgaagggccggttcatcatcttcagagacaattccaagaacacgctgtatctgcaaataaacgccctgagagacgaagacacggccgtttactactgt gcgaaatatgtggaggggtggtcatatacggggggctactacaactcctacgctatggacgtc IGG2 22 213 14 8 9 14 5 8 3 0 13 6 JY8QFUQ01AV8RW 7818 productive Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 954 93.55 203/217 nt NA Homsap IGHJ6*02 F (see comment) 194 83.64 46/55 nt Homsap IGHD1-1*01 F 3 8 8 10 8.8.10 [1.17.38.11] CAREVSDALDVW in-frame + Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ctctggattcatctttgatgattttggcatgagatgggtccgccaagttccagggaaggggctacagtgggtctctggtattaattggaatggtggtaaaacaggttatgcagactctgtgaggggccgattcatcatctccagagacaacgccaagaacgccctgtatctgcaaatgaacagtctcagagccgaggacacggccttatattactgtgcgagagaggtctcggacgccttggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [278] c86>t,T29>I(- - -)|a110>t,Y37>F(- + -)|c120>a,S40>R(- - -)|c134>t,A45>V(+ - +)|g150>a|g151>c,E51>Q(+ + -)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|t256>g,S86>A(- + -)|g282>c|g303>a|c307>t,H103>Y(+ - -)| c86>t,T29>I(- - -)|a110>t,Y37>F(- + -)| c120>a,S40>R(- - -)|c134>t,A45>V(+ - +)|g150>a|g151>c,E51>Q(+ + -)| g191>a,S64>K(- - -)|c192>a,S64>K(- - -)| a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|t256>g,S86>A(- + -)|g282>c|g303>a|c307>t,H103>Y(+ - -)| 320 225 211 14 3 11 1 3 4 0 0 2 1 0 2 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 1 0 0 0 1 0 0 0 0 0 51 51 47 4 1 3 0 1 1 0 0 1 0 0 1 0 0 0 30 24 22 2 0 2 0 1 0 0 0 1 0 0 0 0 0 0 117 114 108 6 2 4 1 1 2 0 0 0 0 0 1 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ctct ggattcatctttgatgattttggc atgagatgggtccgccaagttccagggaaggggctacagtgggtctctggt attaattggaatggtggtaaaaca ggttatgcagactctgtgaggggccgattcatcatctccagagacaacgccaagaacgccctgtatctgcaaatgaacagtctcagagccgaggacacggccttatattactgt gcgagagaggtctcggacgccttggacgtc AREVSDALDV IGHV3-20 IGHD1-1 IGHJ6 ggattcatctttgatgattttggc atgagatgggtccgccaagttccagggaaggggctacagtgggtctctggt attaattggaatggtggtaaaaca ggttatgcagactctgtgaggggccgattcatcatctccagagacaacgccaagaacgccctgtatctgcaaatgaacagtctcagagccgaggacacggccttatattactgt gcgagagaggtctcggacgccttggacgtc IGA1 14 213 8 6 7 11 1 3 3 0 7 4 JY8QFUQ01AVA0Q 13501 productive Homsap IGHV2-5*02 F 1134 96.31 235/244 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD4-17*01 F 2 10 7 13 10.7.13 [9.17.38.11] CAHRGNYGDYVFDSW in-frame + gaccctcacgctgacctgcaccttctctggattctcacttagcactagtgggatgggtgtgggctggatccgtcagcccccaggaaaggccctggaatggcttacactcatttattgggatgatgataagcgctacagcccatctctgaagagcaggctcgccatcaccaaggacacctccaaaaatcaggtggtccttacaatgaccaacatggaccctgtggacacagccacatattattgtgcacacagagggaattacggtgactatgtttttgactcctggggccagggaactctggtcaccgtctcctcagcatccccgaccagccccaaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 89 [315, 314] g81>a|c90>t|a108>g|g109>a,V37>M(+ - -)|g153>a|g160>a,A54>T(- - -)|a229>g,T77>A(- - -)|c255>t|c309>t| g81>a|c90>t|a108>g|g109>a,V37>M(+ - -)| g153>a|g160>a,A54>T(- - -)| a229>g,T77>A(- - -)|c255>t|c309>t| c322>g| 321 (322) 253 (254) 244 9 (10) 6 (7) 3 2 4 3 0 0 0 0 0 0 0 (1) 0 0 78 28 28 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 3 1 1 2 1 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 111 3 2 1 1 0 2 0 0 0 0 0 0 0 0 0 9 (10) 9 9 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 0 (1) 0 0 aa,13-14(FR1)|aa,40-41(FR1)|ta,102-103(CDR1)|aa,141-142(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,238-239(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,267-268(FR3)|aa,270-271(FR3)|aa,277-278(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,10-11(FR1)|tt,73-74(FR1)|tt,82-83(CDR1)|ta,102-103(CDR1)|tt,158-159(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,80-83(CDR1)|ggct,118-121(FR2)|ggct,155-158(FR2)|agca,220-223(FR3)|ggct,224-227(FR3)| aacc,41-44(FR1)|agcc,131-134(FR2)|agcc,202-205(FR3)|aacc,253-256(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)| gaccctcacgctgacctgcaccttctct ggattctcacttagcactagtgggatgggt gtgggctggatccgtcagcccccaggaaaggccctggaatggcttacactc atttattgggatgatgataag cgctacagcccatctctgaagagcaggctcgccatcaccaaggacacctccaaaaatcaggtggtccttacaatgaccaacatggaccctgtggacacagccacatattattgt gcacacagagggaattacggtgactatgtttttgactcc AHRGNYGDYVFDS IGHV2-5 IGHD4-17 IGHJ4 ggattctcacttagcactagtgggatgggt gtgggctggatccgtcagcccccaggaaaggccctggaatggcttacactc atttattgggatgatgataag cgctacagcccatctctgaagagcaggctcgccatcaccaaggacacctccaaaaatcaggtggtccttacaatgaccaacatggaccctgtggacacagccacatattattgt gcacacagagggaattacggtgactatgtttttgactcc IGA1 9 216 9 0 7 7 2 2 3 3 2 1 JY8QFUQ01AVB4I 13177 productive Homsap IGHV3-7*01 F 882 89.86 195/217 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD2-2*01 F 2 8 8 9 8.8.9 [1.17.38.11] CATRSRSPANW in-frame + ctctggattcagttttagtacctattggatgagctgggtccgccaggctccagggaaggggctggaatgggtggccaccataaacgaggatggaagtgacagaacctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaaaaattcattgtatctacaaatgaatagcctgagaaccgacgacacggctcgatattactgtgcgacgcgtagtagaagtccggcgaactggggccagggaagcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 94 [275, 276] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g107>c,S36>T(+ - +)|g153>a|a164>c,N55>T(- + -)|g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a174>g,Q58>E(+ + -)|g192>c,E64>D(+ - +)|a194>g,K65>R(+ + +)|t196>a,Y66>T(+ - -)|a197>c,Y66>T(+ - -)|g252>a|c255>t|c259>t|g267>a|c276>t|g286>a,A96>T(- - -)|g291>c,E97>D(+ - +)|g301>c,V101>R(- - -)|t302>g,V101>R(- - -)|g303>a,V101>R(- - -)| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g107>c,S36>T(+ - +)| g153>a|a164>c,N55>T(- + -)| g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a174>g,Q58>E(+ + -)|g192>c,E64>D(+ - +)|a194>g,K65>R(+ + +)| t196>a,Y66>T(+ - -)|a197>c,Y66>T(+ - -)|g252>a|c255>t|c259>t|g267>a|c276>t|g286>a,A96>T(- - -)|g291>c,E97>D(+ - +)|g301>c,V101>R(- - -)|t302>g,V101>R(- - -)|g303>a,V101>R(- - -)| g317>c,R106>T(- - -)|a318>g,R106>T(- - -)|g319>c|a320>g| 316 (320) 221 (225) 199 22 (26) 6 (8) 16 (18) 2 (4) 5 4 0 2 0 0 1 5 (7) 2 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 1 0 0 0 0 0 1 1 0 0 51 51 49 2 1 1 0 1 0 0 1 0 0 0 0 0 0 0 30 24 19 5 0 5 2 0 0 0 0 0 0 0 2 1 0 0 117 114 102 12 5 7 0 4 3 0 1 0 0 1 2 0 0 1 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (2) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagttttagtacctattgg atgagctgggtccgccaggctccagggaaggggctggaatgggtggccacc ataaacgaggatggaagtgacaga acctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaaaaattcattgtatctacaaatgaatagcctgagaaccgacgacacggctcgatattactgt gcgacgcgtagtagaagtccggcgaac ATRSRSPAN IGHV3-7 IGHD2-2 IGHJ4 ggattcagttttagtacctattgg atgagctgggtccgccaggctccagggaaggggctggaatgggtggccacc ataaacgaggatggaagtgacaga acctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaaaaattcattgtatctacaaatgaatagcctgagaaccgacgacacggctcgatattactgt gcgacgcgtagtagaagtccggcgaac IGG2 22 213 11 11 9 16 2 6 6 0 8 8 JY8QFUQ01AVBM5 16809 productive Homsap IGHV3-11*01 F 846 88.02 191/217 nt NA Homsap IGHJ2*01 F 229 92.45 49/53 nt Homsap IGHD2-8*02 F 3 8 8 12 8.8.12 [1.17.38.11] CADIKLGDWCFDLW in-frame + ctctgaaatcaccttcagcgaccactacatgagctggatccgccaggctccaggaaaggggctggaatggatttcatatattagcaccagtggtaatatgatctattacgcagactctgtgaagggccgattcaccgtctccagggacaacgccaagaggtcgatgtatttgcagatgaacagtctgagagccgaggacacggccgtctatttctgtgccgatataaaactgggtgactggtgcttcgatctctggggccgtggcaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [284] g80>a,G27>E(- - -)|t82>a,F28>I(+ - -)|t105>c|t109>c,Y37>H(+ - -)|g141>a|g153>a|g157>a,V53>I(+ - +)|c165>t|t171>c|g173>c,S58>T(+ - +)|t174>c,S58>T(+ - +)|g188>a,S63>N(- - -)|c191>t,T64>M(- - -)|c192>g,T64>M(- - -)|a195>c|c198>t|a232>g,I78>V(+ - +)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)|a258>g|c259>a,L87>M(+ + -)|c265>t|a270>g|c279>t|g303>c|a308>t,Y103>F(- + -)| g80>a,G27>E(- - -)|t82>a,F28>I(+ - -)|t105>c|t109>c,Y37>H(+ - -)| g141>a|g153>a|g157>a,V53>I(+ - +)|c165>t| t171>c|g173>c,S58>T(+ - +)|t174>c,S58>T(+ - +)|g188>a,S63>N(- - -)|c191>t,T64>M(- - -)|c192>g,T64>M(- - -)|a195>c| c198>t|a232>g,I78>V(+ - +)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)|a258>g|c259>a,L87>M(+ + -)|c265>t|a270>g|c279>t|g303>c|a308>t,Y103>F(- + -)| g315>c|a316>g,R106>D(+ - -)|g317>a,R106>D(+ - -)|a318>t,R106>D(+ - -)|g319>a|a320>t| 314 (320) 219 (225) 193 26 (32) 12 (15) 14 (17) 4 (5) 5 (7) 5 4 1 1 1 (3) 1 2 (3) 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 0 2 0 0 0 1 0 0 0 0 51 51 47 4 3 1 0 3 1 0 0 0 0 0 0 0 0 0 30 24 17 7 2 5 0 1 1 2 1 0 0 0 1 1 0 0 117 114 103 11 6 5 4 0 3 0 0 1 1 0 1 1 0 0 2 (8) 2 2 0 (6) 0 (3) 0 (3) 0 (1) 0 (2) 0 0 0 0 0 (2) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gaaatcaccttcagcgaccactac atgagctggatccgccaggctccaggaaaggggctggaatggatttcatat attagcaccagtggtaatatgatc tattacgcagactctgtgaagggccgattcaccgtctccagggacaacgccaagaggtcgatgtatttgcagatgaacagtctgagagccgaggacacggccgtctatttctgt gccgatataaaactgggtgactggtgcttcgatctc ADIKLGDWCFDL IGHV3-11 IGHD2-8 IGHJ2 gaaatcaccttcagcgaccactac atgagctggatccgccaggctccaggaaaggggctggaatggatttcatat attagcaccagtggtaatatgatc tattacgcagactctgtgaagggccgattcaccgtctccagggacaacgccaagaggtcgatgtatttgcagatgaacagtctgagagccgaggacacggccgtctatttctgt gccgatataaaactgggtgactggtgcttcgatctc IGA1 26 213 18 8 10 15 8 11 9 3 6 8 JY8QFUQ01AVDHQ 18135 productive Homsap IGHV3-9*01 F 945 93.09 202/217 nt NA Homsap IGHJ1*01 F (see comment) 143 75 39/52 nt Homsap IGHD4-17*01 F 2 8 8 10 8.8.10 [1.17.38.11] CARLPGDYGSRW in-frame + Other possibilities: Homsap_IGHJ4*02 and Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctggattcaactttgataaatatgacatgcactgggtccgacaagctccagggaagggcctggagtgggtctcaggtattaagtggaatggcggtcgcgtcggctatgcagactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgagacctgaggacacggccttctattactgtgcaagactccccggtgactacgggtctcgttggggccagggaaccctcatcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [278] c86>a,T29>N(- + -)|g106>a,D36>K(+ - -)|t108>a,D36>K(+ - -)|c113>a,A38>D(- - -)|g129>a|g170>a,S57>K(- - -)|t171>g,S57>K(- - -)|a184>g,S62>G(+ + -)|t186>c,S62>G(+ + -)|a190>c,S64>R(- - -)|a193>g,I65>V(+ - +)|a195>c,I65>V(+ - +)|g204>a|g286>c,A96>P(- - -)|g303>c,L101>F(+ - -)| c86>a,T29>N(- + -)|g106>a,D36>K(+ - -)|t108>a,D36>K(+ - -)|c113>a,A38>D(- - -)| g129>a| g170>a,S57>K(- - -)|t171>g,S57>K(- - -)|a184>g,S62>G(+ + -)|t186>c,S62>G(+ + -)|a190>c,S64>R(- - -)|a193>g,I65>V(+ - +)|a195>c,I65>V(+ - +)| g204>a|g286>c,A96>P(- - -)|g303>c,L101>F(+ - -)| a317>g,K106>R(+ + +)|g319>c,D107>L(- - -)|a320>t,D107>L(- - -)|t321>c,D107>L(- - -)|a322>c| 316 (322) 221 (227) 206 (207) 15 (20) 2 (3) 13 (17) 2 (3) 4 0 1 (2) 2 (3) 2 0 (1) 1 2 (3) 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 0 0 0 2 0 1 0 0 0 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 24 17 7 0 7 2 1 0 1 2 0 0 0 0 0 0 1 117 114 111 3 1 2 0 1 0 0 0 0 0 0 2 0 0 0 4 (10) 4 4 (5) 0 (5) 0 (1) 0 (4) 0 (1) 0 0 0 (1) 0 (1) 0 0 (1) 0 0 (1) 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaactttgataaatatgac atgcactgggtccgacaagctccagggaagggcctggagtgggtctcaggt attaagtggaatggcggtcgcgtc ggctatgcagactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgagacctgaggacacggccttctattactgt gcaagactccccggtgactacgggtctcgt ARLPGDYGSR IGHV3-9 IGHD4-17 IGHJ1 ggattcaactttgataaatatgac atgcactgggtccgacaagctccagggaagggcctggagtgggtctcaggt attaagtggaatggcggtcgcgtc ggctatgcagactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgagacctgaggacacggccttctattactgt gcaagactccccggtgactacgggtctcgt IGA1 15 213 7 8 4 8 3 7 2 0 2 11 JY8QFUQ01AVEUZ 18698 productive (see comment) Homsap IGHV3-48*01 F, or Homsap IGHV3-48*02 F 977 94.5 206/218 nt 94.04 205/218 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD1-7*01 F 3 8 8 10 8.8.10 [1.17.38.11] CARDRNYAFDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 37 of V-REGION: 'G' (1 nucleotides at position 24 to 24 in the user submitted sequence), (cause frameshift); cctctggattcaccttcagcagcGtttagtatgaactgggtccgccaggctccagggaagggactggagtggctttcatacattagtaatactggtagtaacaaatactacgcagactctgtgaagggccgattcaccatctccagagacgatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgtgcgagagatcggaactacgctttcgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [280] t105>c|a110>t,Y37>F(- + -)|c114>t|g147>a|g157>c,V53>L(+ - +)|g173>a,S58>N(- - -)|g176>c,S59>T(+ - +)|a184>g,S62>G(+ + -)|c191>a,T64>N(- + -)|t194>a,I65>K(- + -)|a244>g,N82>D(+ + -)|c287>t,A96>V(+ - +)| t105>c|a110>t,Y37>F(- + -)|c114>t| g147>a|g157>c,V53>L(+ - +)| g173>a,S58>N(- - -)|g176>c,S59>T(+ - +)|a184>g,S62>G(+ + -)|c191>a,T64>N(- + -)|t194>a,I65>K(- + -)| a244>g,N82>D(+ + -)|c287>t,A96>V(+ - +)| 320 226 214 12 3 9 2 2 2 1 0 1 1 1 2 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 1 1 0 0 1 0 0 0 0 0 51 51 49 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 30 24 19 5 0 5 1 1 0 0 0 1 0 1 1 0 0 0 117 114 112 2 0 2 1 0 1 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagcagctttagt atgaactgggtccgccaggctccagggaagggactggagtggctttcatac attagtaatactggtagtaacaaa tactacgcagactctgtgaagggccgattcaccatctccagagacgatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgt gcgagagatcggaactacgctttcgactac ARDRNYAFDY IGHV3-48 IGHD1-7 IGHJ4 ggattcaccttcagcagctttagt atgaactgggtccgccaggctccagggaagggactggagtggctttcatac attagtaatactggtagtaacaaa tactacgcagactctgtgaagggccgattcaccatctccagagacgatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgt gcgagagatcggaactacgctttcgactac IGA2 12 213 7 5 4 7 3 5 1 2 3 6 JY8QFUQ01AVHAA 12756 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F or Homsap IGHV3-48*02 F or Homsap IGHV3-48*04 F 810 86.18 187/217 nt NA Homsap IGHJ6*02 F 229 85.48 53/62 nt Homsap IGHD3-16*02 F 3 8 8 23 8.8.23 [1.17.38.11] CARDRRVFFGQLITTKIYHYGMDVW in-frame - ctctggattcaacttcaattattatagcatgagttgggtccgccaggctccgggaaagggactggagtgggtttcatacattagtactagtagtagttacatgtattacacggactccgtgaagggccgcttcaccgtctccagagacaacgccaagaaatctctgtatctggaaatgaacagcctaagggacgaggacacggctgtctacttttgtgcgagagatcgacgagtgttctttggacaattaataaccaccaaaatctaccactacggcatggacgtctggggccaagggaccacggtcatcgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [318] c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c108>t,S36>Y(+ - -)|a119>g,N40>S(- - -)|c120>t,N40>S(- - -)|a138>g|g141>a|g147>a|c159>t|c164>a,S55>Y(+ - -)|g173>c,S58>T(+ - +)|a195>g,I65>M(+ + -)|c198>t|g202>a,A68>T(- - -)|a204>g,A68>T(- - -)|a210>c|a225>c|a232>g,I78>V(+ - +)|c255>a,N85>K(+ - -)|a258>t|c268>g,Q90>E(+ + -)|g282>a|a285>g|c287>a,A96>D(- - -)|g303>c|t306>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c108>t,S36>Y(+ - -)| a119>g,N40>S(- - -)|c120>t,N40>S(- - -)|a138>g|g141>a|g147>a|c159>t|c164>a,S55>Y(+ - -)| g173>c,S58>T(+ - +)|a195>g,I65>M(+ + -)| c198>t|g202>a,A68>T(- - -)|a204>g,A68>T(- - -)|a210>c|a225>c|a232>g,I78>V(+ - +)|c255>a,N85>K(+ - -)|a258>t|c268>g,Q90>E(+ + -)|g282>a|a285>g|c287>a,A96>D(- - -)|g303>c|t306>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| 320 225 195 30 12 18 6 6 5 1 2 4 3 0 2 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 2 1 0 0 1 1 0 0 0 0 0 51 51 44 7 4 3 2 2 2 0 0 1 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 117 114 98 16 8 8 3 2 2 1 2 2 2 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaacttcaattattatagc atgagttgggtccgccaggctccgggaaagggactggagtgggtttcatac attagtactagtagtagttacatg tattacacggactccgtgaagggccgcttcaccgtctccagagacaacgccaagaaatctctgtatctggaaatgaacagcctaagggacgaggacacggctgtctacttttgt gcgagagatcgacgagtgttctttggacaattaataaccaccaaaatctaccactacggcatggacgtc ARDRRVFFGQLITTKIYHYGMDV IGHV3-21 IGHD3-16 IGHJ6 ggattcaacttcaattattatagc atgagttgggtccgccaggctccgggaaagggactggagtgggtttcatac attagtactagtagtagttacatg tattacacggactccgtgaagggccgcttcaccgtctccagagacaacgccaagaaatctctgtatctggaaatgaacagcctaagggacgaggacacggctgtctacttttgt gcgagagatcgacgagtgttctttggacaattaataaccaccaaaatctaccactacggcatggacgtc IGG1 30 213 18 12 11 18 7 12 12 0 11 7 JY8QFUQ01AVLE1 21486 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 873 89.4 194/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-10*01 F 3 8 8 10 8.8.10 [1.17.38.11] CSKDTFGREDYW in-frame + ctctggattctccgtcagtaattactggatgcactgggtccgccaggctccaggggaggggctggagtgggtctcacgtattaatgaagatggtagtcggacagactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgtagtaaagacacatttgggcgagaagactactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttc NA IGA2 100 100 [278] a85>t,T29>S(+ - +)|t88>g,F30>V(+ - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a132>g|a142>g,K48>E(+ - -)|t152>a,V51>E(- + -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>t|a190>c,S64>R(- - -)|c192>g,S64>R(- - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c234>t|c236>a,S79>Y(+ - -)|a245>g,N82>S(- - -)|g258>a|c287>t,A96>V(+ - +)|g291>a|g303>c|c309>t| a85>t,T29>S(+ - +)|t88>g,F30>V(+ - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a132>g|a142>g,K48>E(+ - -)|t152>a,V51>E(- + -)| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>t|a190>c,S64>R(- - -)|c192>g,S64>R(- - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c234>t|c236>a,S79>Y(+ - -)|a245>g,N82>S(- - -)|g258>a|c287>t,A96>V(+ - +)|g291>a|g303>c|c309>t| g313>a,A105>S(- + -)|c314>g,A105>S(- + -)|a315>t,A105>S(- + -)|g317>a,R106>K(+ + +)| 320 225 198 27 7 20 5 7 4 0 1 1 2 2 1 2 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 1 0 0 0 1 0 0 0 0 1 51 51 48 3 1 2 2 0 0 0 0 0 0 1 0 0 0 0 30 24 18 6 1 5 1 1 0 0 1 0 0 1 0 1 1 0 117 114 104 10 5 5 2 3 3 0 0 1 0 0 1 0 0 0 8 8 4 4 0 4 0 2 0 0 0 0 1 0 0 1 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattctccgtcagtaattactgg atgcactgggtccgccaggctccaggggaggggctggagtgggtctcacgt attaatgaagatggtagtcggaca gactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgt agtaaagacacatttgggcgagaagactac SKDTFGREDY IGHV3-74 IGHD3-10 IGHJ4 ggattctccgtcagtaattactgg atgcactgggtccgccaggctccaggggaggggctggagtgggtctcacgt attaatgaagatggtagtcggaca gactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgt agtaaagacacatttgggcgagaagactac IGA2 23 213 14 9 9 13 5 10 6 1 7 9 JY8QFUQ01AVMZN 15148 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F (see comment) 712 80.82 177/219 nt 79 173/219 nt Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD6-6*01 F 3 8 7 9 8.7.9 [3.17.38.11] CARLDGHFDLW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (80,82% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR1-IMGT at codon 36 of V-REGION: 'C' (1 nucleotides at position 25 to 25 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 41 of V-REGION: 'C' (1 nucleotides at position 41 to 41 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 68 of V-REGION: 'G' (1 nucleotides at position 115 to 115 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 72 of V-REGION: 'G' (1 nucleotides at position 128 to 128 in the user submitted sequence), (cause frameshift); gtagtctctgggttcaccatcagtCacgtactccatgggcCtgggtccgccaggctccgggaaaggggctggagtgggtctcattaatttatcccggcgggagcacattccacgGcagactccgtgaGgggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgtgcgagactcgacgggcacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttcccccctggcaccctcctccaagagcacctctggggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [283, 282] c71>t,A24>V(+ - +)|c74>t,A25>V(+ - +)|g88>a,V30>I(+ - +)|g107>c,S36>T(+ - +)|c108>g,S36>T(+ - +)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)|a118>g,S40>G(+ + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g|a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c222>g|a225>g|t226>c,F76>L(+ - -)|c228>t,F76>L(+ - -)|c230>t,T77>I(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| c71>t,A24>V(+ - +)|c74>t,A25>V(+ - +)| g88>a,V30>I(+ - +)|g107>c,S36>T(+ - +)|c108>g,S36>T(+ - +)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)| a118>g,S40>G(+ + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g| a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c222>g|a225>g|t226>c,F76>L(+ - -)|c228>t,F76>L(+ - -)|c230>t,T77>I(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| g319>c|a320>t| 318 (320) 225 (227) 183 42 (44) 9 (11) 33 9 7 7 3 2 2 2 (3) 2 2 (3) 4 1 1 78 9 7 2 0 2 0 0 2 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 1 0 0 1 0 1 0 1 1 0 0 51 51 46 5 2 3 2 1 0 0 0 0 0 1 0 0 1 0 30 21 17 4 2 2 0 0 0 1 1 0 0 0 1 0 0 1 117 114 88 26 5 21 7 5 5 2 0 2 1 1 0 3 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtagtctct gggttcaccatcagtacgtactcc atgggctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgagggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc ARLDGHFDL IGHV3-53 IGHD6-6 IGHJ2 gggttcaccatcagtacgtactcc atgggctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgagggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc IGG1 40 210 24 16 12 21 12 19 7 2 24 7 JY8QFUQ01AVNE7 20282 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 964 93.61 205/219 nt 92.69 203/219 nt Homsap IGHJ2*01 F 220 90.57 48/53 nt Homsap IGHD1-26*01 F 3 8 8 12 8.8.12 [2.17.38.11] CARPFSGRWCFDLW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 53 of V-REGION: 'G' (1 nucleotides at position 75 to 75 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 56 of V-REGION: 'AT' (2 nucleotides at position 84 to 85 in the user submitted sequence), (cause frameshift); gcctctggattcaccttcagttactcctggatgcactgggtccgccaagttccaggaaaggggccggtgtgggtGctcacgaaATtcaaaagtgatgggagtaccccaagttacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagtcgaggacacggctgtttattactgtgcaagaccgtttagtgggaggtggtgcttcgatctctggggccgtggcaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccca NA IGA2 93 100 [289, 290] a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a110>c,Y37>S(+ - -)|c134>t,A45>V(+ - +)|g141>a|t149>c,L50>P(- - -)|t165>a|t168>c|t171>a,N57>K(+ - -)|g191>c,S64>T(+ - +)|a193>c,T65>P(+ + -)|c198>t|c287>t,A96>V(+ - +)|g303>t| a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a110>c,Y37>S(+ - -)| c134>t,A45>V(+ - +)|g141>a|t149>c,L50>P(- - -)|t165>a| t168>c|t171>a,N57>K(+ - -)|g191>c,S64>T(+ - +)|a193>c,T65>P(+ + -)| c198>t|c287>t,A96>V(+ - +)|g303>t| g319>c|a320>c| 318 (320) 225 (227) 211 14 (16) 5 (7) 9 0 2 3 2 2 (3) 0 1 2 1 (2) 0 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 1 0 1 0 0 0 0 0 51 51 47 4 2 2 0 1 1 1 0 0 0 1 0 0 0 0 30 24 20 4 1 3 0 0 0 1 1 0 0 1 1 0 0 0 117 114 111 3 2 1 0 0 2 0 0 0 0 0 0 0 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcaccttcagttactcctgg atgcactgggtccgccaagttccaggaaaggggccggtgtgggtctcacga atcaaaagtgatgggagtacccca agttacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagtcgaggacacggctgtttattactgt gcaagaccgtttagtgggaggtggtgcttcgatctc ARPFSGRWCFDL IGHV3-74 IGHD1-26 IGHJ2 ggattcaccttcagttactcctgg atgcactgggtccgccaagttccaggaaaggggccggtgtgggtctcacga atcaaaagtgatgggagtacccca agttacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagtcgaggacacggctgtttattactgt gcaagaccgtttagtgggaggtggtgcttcgatctc IGA2 14 213 7 7 5 7 2 7 4 1 3 6 JY8QFUQ01AVOVU 9360 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 1008 98.56 205/208 nt NA Homsap IGHJ6*02 F 229 85.48 53/62 nt Homsap IGHD4-17*01 F 2 6 8 16 6.8.16 [X.17.38.11] CARQLDYGDYSRHGLDVW in-frame + caactttaccggctactggatcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatcatctatcctggtgactctgataccagatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcagcaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccgtgtattactgtgcgagacaattagactacggtgactactctaggcacggtttggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [287] g86>a,S29>N(- - -)|a106>g,S36>G(+ + -)|a301>g,M101>V(+ - -)| g86>a,S29>N(- - -)|a106>g,S36>G(+ + -)| a301>g,M101>V(+ - -)| 320 216 213 3 0 3 2 1 0 0 0 0 0 0 0 0 0 0 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 19 17 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 113 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| caactttaccggctactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgatacc agatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcagcaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccgtgtattactgt gcgagacaattagactacggtgactactctaggcacggtttggacgtc ARQLDYGDYSRHGLDV IGHV5-51 IGHD4-17 IGHJ6 caactttaccggctactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgatacc agatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcagcaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccgtgtattactgt gcgagacaattagactacggtgactactctaggcacggtttggacgtc IGA2 3 208 3 0 1 1 2 2 0 0 1 2 JY8QFUQ01AVPMG 6871 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 956 92.76 205/221 nt NA Homsap IGHJ5*02 F (see comment) 147 76.47 39/51 nt Homsap IGHD3-10*01 F 2 10 7 13 10.7.13 [1.17.38.11] CARRGSYGSGSYYPW in-frame + Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) tctctggtggcgccatcagcagtaatagttactactgggactggatccgccagcccccagggaaggggctggagtggattgggagtatgttttatactggggtcaccttctacaacccgtccctcaagagtcgagttaacatttccgtggacacgtccaagagccagttctccctgaggctgagctctgtgaccgccgcagacacggctgtgtatcactgtgcgagacgtggttcctatggttcggggagttattatccctggggccggggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 100 100 [291] t85>g,S29>A(- + -)|g104>a,S35>N(- - -)|g119>a,G40>D(- - -)|c168>g,I56>M(+ + -)|a170>t,Y57>F(- + -)|g176>c,S59>T(+ - +)|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|a197>t,Y66>F(- + -)|c228>t|c230>a,T77>N(- + -)|a234>t|a240>g|a254>g,N85>S(- - -)|a269>g,K90>R(+ + +)|t307>c,Y103>H(+ - -)| t85>g,S29>A(- + -)|g104>a,S35>N(- - -)| g119>a,G40>D(- - -)| c168>g,I56>M(+ + -)|a170>t,Y57>F(- + -)|g176>c,S59>T(+ - +)|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)| a197>t,Y66>F(- + -)|c228>t|c230>a,T77>N(- + -)|a234>t|a240>g|a254>g,N85>S(- - -)|a269>g,K90>R(+ + +)|t307>c,Y103>H(+ - -)| a320>g| 319 (320) 228 (229) 212 16 (17) 3 (4) 13 4 (5) 2 1 1 0 1 3 0 1 1 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 21 16 5 0 5 1 0 0 0 0 0 1 0 1 1 1 0 117 114 106 8 3 5 3 0 1 1 0 1 2 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggcgccatcagcagtaatagttactac tgggactggatccgccagcccccagggaaggggctggagtggattgggagt atgttttatactggggtcacc ttctacaacccgtccctcaagagtcgagttaacatttccgtggacacgtccaagagccagttctccctgaggctgagctctgtgaccgccgcagacacggctgtgtatcactgt gcgagacgtggttcctatggttcggggagttattatccc ARRGSYGSGSYYP IGHV4-39 IGHD3-10 IGHJ5 ggtggcgccatcagcagtaatagttactac tgggactggatccgccagcccccagggaaggggctggagtggattgggagt atgttttatactggggtcacc ttctacaacccgtccctcaagagtcgagttaacatttccgtggacacgtccaagagccagttctccctgaggctgagctctgtgaccgccgcagacacggctgtgtatcactgt gcgagacgtggttcctatggttcggggagttattatccc IGA2 16 216 8 8 3 7 5 9 3 0 6 7 JY8QFUQ01AVQ0D 9809 productive (see comment) Homsap IGHV1-46*01 F, or Homsap IGHV1-46*03 F 1011 97.2 208/214 nt 96.73 207/214 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-13*01 F 3 8 8 11 8.8.11 [0.17.38.11] CARGQQLRDFDYW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 66 of V-REGION: 'A' (1 nucleotides at position 102 to 102 in the user submitted sequence), (cause frameshift); tggatacaccttcaccagctactatttgcactgggtgcgacaggcccctggacaagggcttgagtggatgggaataatcgaccctagtggtggtgccacaaAgctacgcacagcagttccagggcagagtcaccatgaccagggacacgtccacgagcacagtctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgtgcgaggggacagcagcttcgagactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [279] a115>t,M39>L(+ + -)|a169>g,N57>D(+ + -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|a208>c,K70>Q(+ - -)|g291>a| a115>t,M39>L(+ + -)| a169>g,N57>D(+ + -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| a208>c,K70>Q(+ - -)|g291>a| a318>g|a320>g| 317 (320) 219 (222) 213 (214) 6 (8) 1 (3) 5 2 (4) 1 0 0 1 0 1 0 1 0 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 30 24 21 3 0 3 2 0 0 0 0 0 0 0 1 0 0 0 117 114 112 2 1 1 0 1 0 0 1 0 0 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,162-163(FR2)|ta,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,62-63(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,164-165(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggca,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|aacc,169-172(CDR2)|agct,196-199(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcaccagctactat ttgcactgggtgcgacaggcccctggacaagggcttgagtggatgggaata atcgaccctagtggtggtgccaca agctacgcacagcagttccagggcagagtcaccatgaccagggacacgtccacgagcacagtctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcgaggggacagcagcttcgagactttgactac ARGQQLRDFDY IGHV1-46 IGHD6-13 IGHJ4 ggatacaccttcaccagctactat ttgcactgggtgcgacaggcccctggacaagggcttgagtggatgggaata atcgaccctagtggtggtgccaca agctacgcacagcagttccagggcagagtcaccatgaccagggacacgtccacgagcacagtctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcgaggggacagcagcttcgagactttgactac IGA1 6 213 3 3 1 2 2 4 1 0 2 3 JY8QFUQ01AVQBY 9795 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 927 92.17 200/217 nt NA Homsap IGHJ3*02 F 214 92 46/50 nt Homsap IGHD7-27*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARDQSLGMPEAFDIW in-frame + ctctggattcaccttcagtcgctatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcaactattagtagtcatagtatttacatatactatgcagactcagtggagggccgattcaccgtctccagagacaacgccgagaactcgctgtatctgcacatgaacaccctcagagccgacgacacggctatatattactgtgcgagagatcaaagtctggggatgcccgaggcttttgacatctggggccaagggacaatggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [291] a106>c,S36>R(- - -)|t163>a,S55>T(+ - +)|c165>t,S55>T(+ - +)|a175>c,S59>H(+ - -)|g176>a,S59>H(+ - -)|g188>t,S63>I(- - -)|c201>t|a214>g,K72>E(+ - -)|a232>g,I78>V(+ - +)|a250>g,K84>E(+ - -)|a258>g|a270>c,Q90>H(- + -)|g278>c,S93>T(+ - +)|g282>c|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| a106>c,S36>R(- - -)| t163>a,S55>T(+ - +)|c165>t,S55>T(+ - +)| a175>c,S59>H(+ - -)|g176>a,S59>H(+ - -)|g188>t,S63>I(- - -)| c201>t|a214>g,K72>E(+ - -)|a232>g,I78>V(+ - +)|a250>g,K84>E(+ - -)|a258>g|a270>c,Q90>H(- + -)|g278>c,S93>T(+ - +)|g282>c|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 225 208 17 3 14 4 3 2 0 3 0 0 1 3 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 1 0 0 0 0 1 0 0 0 0 30 24 21 3 0 3 0 1 0 0 1 0 0 0 0 0 1 0 117 114 103 11 3 8 4 2 1 0 1 0 0 0 3 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtcgctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtagtcatagtatttacata tactatgcagactcagtggagggccgattcaccgtctccagagacaacgccgagaactcgctgtatctgcacatgaacaccctcagagccgacgacacggctatatattactgt gcgagagatcaaagtctggggatgcccgaggcttttgacatc ARDQSLGMPEAFDI IGHV3-21 IGHD7-27 IGHJ3 ggattcaccttcagtcgctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtagtcatagtatttacata tactatgcagactcagtggagggccgattcaccgtctccagagacaacgccgagaactcgctgtatctgcacatgaacaccctcagagccgacgacacggctatatattactgt gcgagagatcaaagtctggggatgcccgaggcttttgacatc IGG1 17 213 9 8 5 9 4 8 3 0 10 4 JY8QFUQ01AVT39 10224 productive Homsap IGHV3-7*03 F 837 87.56 190/217 nt NA Homsap IGHJ6*03 F (see comment) 183 84.31 43/51 nt Homsap IGHD6-6*01 F 3 8 8 8 8.8.8 [1.17.38.11] CTRRGFMDVW in-frame + Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) ctccggattcacttttagtgactattggatgagttgggtccgccaggctccagggaagggactggagtgggtggccaccacaaacgaggacgagactaagaaatactctgcggactctgtgaggggccgattcaccatctccagagacaacgccaagaactcactgtacttgcagatgagcagcctgagagccgacgacgcggccgtctattattgtacgagaagaggattcatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgcaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccag NA IGA2 71 95 [272, 274] t78>c|c87>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c120>t|g147>a|a164>c,N55>T(- + -)|t167>c,I56>T(- - -)|g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a174>g,Q58>E(+ + -)|t177>c|g185>a,G62>E(- - -)|a186>g,G62>E(- - -)|g188>c,S63>T(+ - +)|g190>a,E64>K(+ - -)|a200>c,Y67>S(+ - -)|t203>c,V68>A(+ - +)|a215>g,K72>R(+ + +)|t264>c|c265>t|a270>g|a275>g,N92>S(- - -)|g291>c,E97>D(+ - +)|a295>g,T99>A(- - -)|g303>c|c309>t|g313>a,A105>T(- - -)| t78>c| c87>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| c120>t|g147>a|a164>c,N55>T(- + -)| t167>c,I56>T(- - -)|g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a174>g,Q58>E(+ + -)|t177>c|g185>a,G62>E(- - -)|a186>g,G62>E(- - -)|g188>c,S63>T(+ - +)|g190>a,E64>K(+ - -)| a200>c,Y67>S(+ - -)|t203>c,V68>A(+ - +)|a215>g,K72>R(+ + +)|t264>c|c265>t|a270>g|a275>g,N92>S(- - -)|g291>c,E97>D(+ - +)|a295>g,T99>A(- - -)|g303>c|c309>t| g313>a,A105>T(- - -)|g319>a|a320>g| 318 (320) 223 (225) 195 28 (30) 10 (12) 18 7 (8) 5 (6) 4 5 2 0 0 0 4 1 0 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 21 3 1 2 1 1 1 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 1 0 0 0 0 0 0 0 30 24 15 9 1 8 2 2 0 2 0 0 0 0 2 1 0 0 117 114 103 11 5 6 4 0 2 2 1 0 0 0 2 0 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 (1) 1 (2) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcacttttagtgactattgg atgagttgggtccgccaggctccagggaagggactggagtgggtggccacc acaaacgaggacgagactaagaaa tactctgcggactctgtgaggggccgattcaccatctccagagacaacgccaagaactcactgtacttgcagatgagcagcctgagagccgacgacgcggccgtctattattgt acgagaagaggattcatggacgtc TRRGFMDV IGHV3-7 IGHD6-6 IGHJ6 ggattcacttttagtgactattgg atgagttgggtccgccaggctccagggaagggactggagtgggtggccacc acaaacgaggacgagactaagaaa tactctgcggactctgtgaggggccgattcaccatctccagagacaacgccaagaactcactgtacttgcagatgagcagcctgagagccgacgacgcggccgtctattattgt acgagaagaggattcatggacgtc IGA2 26 213 19 7 8 13 11 13 7 2 7 10 JY8QFUQ01AVZKB 17863 productive Homsap IGHV3-11*01 F (see comment) 756 83.41 181/217 nt NA Homsap IGHJ5*02 F (see comment) 129 72.55 37/51 nt Homsap IGHD2-8*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARGRGRTNSPVDLW in-frame + Low V-REGION identity (83,41% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctggattcaccctcagtgactacagtatgagttgggtccgccaggctccagggaaggggctggagtgggtctcatacatcagccgaagtggaagtaatgtggaaactgcggactctgtgaggggccgattcaccgcctccagggacaccgccaataattcactgtttctgcggatgaatagcctgacagtcgaggacacggccctctattactgtgcgagaggtagggggcgcactaatagtccagttgacctctggggccagggaacccaggtcaccgtctccgtcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgctgcctggtccagggcttcttcccccag NA IGA1 79 89 [288] t88>c,F30>L(+ - -)|t112>a,Y38>S(+ - -)|a113>g,Y38>S(+ - -)|c114>t,Y38>S(+ - -)|c120>t|a124>g,I42>V(+ - +)|t159>c|t168>c|t171>c|a172>c,S58>R(- - -)|t174>a,S58>R(- - -)|t186>a|c191>a,T64>N(- + -)|c192>t,T64>N(- + -)|a193>g,I65>V(+ - +)|a195>g,I65>V(+ - +)|t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|t199>a,Y67>T(+ - -)|a200>c,Y67>T(+ - -)|c201>t,Y67>T(+ - -)|a204>g|a215>g,K72>R(+ + +)|a232>g,I78>A(+ - +)|t233>c,I78>A(+ - +)|a245>c,N82>T(- + -)|g252>t,K84>N(+ - -)|c255>t|a263>t,Y88>F(- + -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>t|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)| t88>c,F30>L(+ - -)|t112>a,Y38>S(+ - -)|a113>g,Y38>S(+ - -)|c114>t,Y38>S(+ - -)| c120>t|a124>g,I42>V(+ - +)|t159>c| t168>c|t171>c|a172>c,S58>R(- - -)|t174>a,S58>R(- - -)|t186>a|c191>a,T64>N(- + -)|c192>t,T64>N(- + -)|a193>g,I65>V(+ - +)|a195>g,I65>V(+ - +)| t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|t199>a,Y67>T(+ - -)|a200>c,Y67>T(+ - -)|c201>t,Y67>T(+ - -)|a204>g|a215>g,K72>R(+ + +)|a232>g,I78>A(+ - +)|t233>c,I78>A(+ - +)|a245>c,N82>T(- + -)|g252>t,K84>N(+ - -)|c255>t|a263>t,Y88>F(- + -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>t|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)| a320>g| 319 (320) 224 (225) 188 36 (37) 8 (9) 28 9 (10) 0 7 5 3 2 1 4 3 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 0 1 1 0 0 0 1 0 0 0 0 51 51 48 3 2 1 1 0 1 1 0 0 0 0 0 0 0 0 30 24 15 9 3 6 2 0 1 2 1 1 0 2 0 0 0 0 117 114 94 20 3 17 5 0 4 1 2 1 1 1 3 0 1 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccctcagtgactacagt atgagttgggtccgccaggctccagggaaggggctggagtgggtctcatac atcagccgaagtggaagtaatgtg gaaactgcggactctgtgaggggccgattcaccgcctccagggacaccgccaataattcactgtttctgcggatgaatagcctgacagtcgaggacacggccctctattactgt gcgagaggtagggggcgcactaatagtccagttgacctc ARGRGRTNSPVDL IGHV3-11 IGHD2-8 IGHJ5 ggattcaccctcagtgactacagt atgagttgggtccgccaggctccagggaaggggctggagtgggtctcatac atcagccgaagtggaagtaatgtg gaaactgcggactctgtgaggggccgattcaccgcctccagggacaccgccaataattcactgtttctgcggatgaatagcctgacagtcgaggacacggccctctattactgt gcgagaggtagggggcgcactaatagtccagttgacctc IGA1 36 213 21 15 7 13 14 23 5 3 18 10 JY8QFUQ01AW02O 15330 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 828 87.1 189/217 nt NA Homsap IGHJ3*01 F 187 86 43/50 nt Homsap IGHD3-16*01 F 3 8 8 18 8.8.18 [1.17.38.11] CASLNTHAGPGAERSAFDVW in-frame + ctctggattcatcttcagtaactatagaatgaactgggtccgtcaggctccagggaaggggctcgagtgggtctcgtccatcaccagttccagtagttacatctactatgcagactcggtgacgggccgattcaccatctccagagacaactccaaggggtcactctatctgcacatgaacgaccttagggccgaagacacggctgtctattactgtgcgagcctaaacacgcacgcgggaccgggagccgagaggtctgcttttgatgtctggggccgagggacaagggtcaccgtctcctcaccatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggccttc NA IGA1 100 100 [302] c86>t,T29>I(- - -)|g107>a,S36>N(- - -)|c114>a,S38>R(- - -)|c129>t|g150>c|a162>g|t168>c|g170>c,S57>T(+ - +)|t171>c,S57>T(+ - +)|a175>t|g176>c|t177>c|a195>c|c201>t|a210>g|a215>c,K72>T(- - -)|g247>t,A83>S(- + -)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|g261>c|a270>c,Q90>H(- + -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g282>t|a285>g|g291>a|g303>c| c86>t,T29>I(- - -)|g107>a,S36>N(- - -)|c114>a,S38>R(- - -)| c129>t|g150>c|a162>g| t168>c|g170>c,S57>T(+ - +)|t171>c,S57>T(+ - +)|a175>t|g176>c|t177>c|a195>c| c201>t|a210>g|a215>c,K72>T(- - -)|g247>t,A83>S(- + -)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|g261>c|a270>c,Q90>H(- + -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g282>t|a285>g|g291>a|g303>c| a318>c,R106>S(- - -)|g319>c|a320>t| 317 (320) 222 (225) 194 28 (31) 15 (17) 13 (14) 6 3 3 3 3 (4) 1 1 (2) 0 5 (6) 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 1 0 0 1 0 0 0 0 0 0 51 51 48 3 3 0 1 0 1 0 0 0 0 0 1 0 0 0 30 24 17 7 5 2 0 0 0 3 1 0 1 0 2 0 0 0 117 114 99 15 7 8 5 2 1 0 2 0 0 0 2 1 2 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 0 0 0 0 (1) 0 0 (1) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagtaactataga atgaactgggtccgtcaggctccagggaaggggctcgagtgggtctcgtcc atcaccagttccagtagttacatc tactatgcagactcggtgacgggccgattcaccatctccagagacaactccaaggggtcactctatctgcacatgaacgaccttagggccgaagacacggctgtctattactgt gcgagcctaaacacgcacgcgggaccgggagccgagaggtctgcttttgatgtc ASLNTHAGPGAERSAFDV IGHV3-21 IGHD3-16 IGHJ3 ggattcatcttcagtaactataga atgaactgggtccgtcaggctccagggaaggggctcgagtgggtctcgtcc atcaccagttccagtagttacatc tactatgcagactcggtgacgggccgattcaccatctccagagacaactccaaggggtcactctatctgcacatgaacgaccttagggccgaagacacggctgtctattactgt gcgagcctaaacacgcacgcgggaccgggagccgagaggtctgcttttgatgtc IGA1 28 213 15 13 6 15 9 13 10 5 8 5 JY8QFUQ01AW9GE 17831 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*02 F or Homsap IGHV4-39*05 F (see comment) 758 82.81 183/221 nt NA Homsap IGHJ4*02 F (see comment) 141 77.08 37/48 nt Homsap IGHD3-3*01 F 2 10 7 12 10.7.12 [1.17.38.11] CARRWSGTYPFASW in-frame + Low V-REGION identity (82,81% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) tctctggtggctcactcagaagtagtagtcaccattggggctggattcgtcagtcccccgggaaggggctggagtggcttgggactgtcgactttcgtgggaccacccactacaacccgtccctcatgggtcgactcacgatatccgtcgacgcgcccaagagtcaaatgtccctgcacttgagctctgtgaccgccgcagacacggctttttacttctgtgcgagacgctggagtgggacttatccgtttgcctcctggggccagggcatcccggtcatcgtctccccagcttccaccaagggcccatccgtcttcccccgtggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcag NA IGG4 93 75 [290, 281, 289] c87>a|a88>c,I30>L(+ + +)|c93>a,S31>R(- - -)|t109>c,Y37>H(+ - -)|t112>c,Y38>H(+ - -)|c114>t,Y38>H(+ - -)|c126>t|c129>t|c133>t,P45>S(+ - -)|a138>c|a157>c,I53>L(+ + +)|g164>c,S55>T(+ - +)|a166>g,I56>V(+ - +)|t169>g,Y57>D(- - -)|t171>c,Y57>D(- - -)|a173>t,Y58>F(- + -)|a175>c,S59>R(- - -)|g191>c,S64>T(+ - +)|t196>c,Y66>H(+ - -)|a215>t,K72>M(- + -)|a220>g,S74>G(+ + -)|g226>c,V76>L(+ - +)|c231>g|a240>c|a244>g,T82>A(- - -)|t247>c,S83>P(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|t259>a,F87>M(+ - -)|c261>g,F87>M(+ - -)|a268>c,K90>H(- - +)|g270>c,K90>H(- - +)|c271>t|g301>t,V101>F(+ - -)|g303>t,V101>F(+ - -)|t306>c|a308>t,Y103>F(- + -)| c87>a|a88>c,I30>L(+ + +)|c93>a,S31>R(- - -)|t109>c,Y37>H(+ - -)|t112>c,Y38>H(+ - -)|c114>t,Y38>H(+ - -)| c126>t|c129>t|c133>t,P45>S(+ - -)|a138>c|a157>c,I53>L(+ + +)|g164>c,S55>T(+ - +)| a166>g,I56>V(+ - +)|t169>g,Y57>D(- - -)|t171>c,Y57>D(- - -)|a173>t,Y58>F(- + -)|a175>c,S59>R(- - -)|g191>c,S64>T(+ - +)| t196>c,Y66>H(+ - -)|a215>t,K72>M(- + -)|a220>g,S74>G(+ + -)|g226>c,V76>L(+ - +)|c231>g|a240>c|a244>g,T82>A(- - -)|t247>c,S83>P(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|t259>a,F87>M(+ - -)|c261>g,F87>M(+ - -)|a268>c,K90>H(- - +)|g270>c,K90>H(- - +)|c271>t|g301>t,V101>F(+ - -)|g303>t,V101>F(+ - -)|t306>c|a308>t,Y103>F(- + -)| a320>g| 319 (320) 228 (229) 190 38 (39) 9 (10) 29 4 (5) 1 6 6 6 2 3 1 4 2 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 1 5 0 0 1 2 1 2 0 0 0 0 0 0 51 51 45 6 3 3 0 0 3 0 2 0 0 0 1 0 0 0 30 21 15 6 0 6 1 0 0 1 1 0 1 0 1 0 0 1 117 114 94 20 5 15 3 1 2 3 2 0 2 1 2 2 2 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctcactcagaagtagtagtcaccat tggggctggattcgtcagtcccccgggaaggggctggagtggcttgggact gtcgactttcgtgggaccacc cactacaacccgtccctcatgggtcgactcacgatatccgtcgacgcgcccaagagtcaaatgtccctgcacttgagctctgtgaccgccgcagacacggctttttacttctgt gcgagacgctggagtgggacttatccgtttgcctcc ARRWSGTYPFAS IGHV4-39 IGHD3-3 IGHJ4 ggtggctcactcagaagtagtagtcaccat tggggctggattcgtcagtcccccgggaaggggctggagtggcttgggact gtcgactttcgtgggaccacc cactacaacccgtccctcatgggtcgactcacgatatccgtcgacgcgcccaagagtcaaatgtccctgcacttgagctctgtgaccgccgcagacacggctttttacttctgt gcgagacgctggagtgggacttatccgtttgcctcc IGG4 38 216 17 21 7 17 10 21 8 1 18 11 JY8QFUQ01AWGJ4 11159 productive Homsap IGHV1-3*01 F 876 90.19 193/214 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD6-25*01 F 1 8 8 13 8.8.13 [0.17.38.11] CARDGYRSGNGADYW in-frame + tggatacagcttcactaactacaatatccattgggtgcgccaggcccccggacaagggcttgagtgggtgggatggatcaacgctggcaatggcaatacaagatattcacagaaattgcagggcagagtcaccatttccagggacacatccgcgagcattgccaacatggagttgagcagcctgagatatgaagacacggctgtatattattgtgcgagagacggctataggagtggcaacggggctgactattggggccagggaaccctggtcaccgtttccccagcatccccgaccagccccaaaggtcttcccgctgagccctcgacagcacccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccca NA IGA2 79 84 [287, 286, 284, 285] c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|t111>c|g112>a,A38>N(- - -)|c113>a,A38>N(- - -)|g117>c,M39>I(+ + -)|a145>g,R49>G(- - -)|a157>g,M53>V(+ - -)|t189>c|c192>t|a197>g,K66>R(+ + +)|g210>a|c213>g,F71>L(+ - -)|a235>t,T79>S(+ - +)|c257>t,T86>I(- - -)|a258>t,T86>I(- - -)|t262>a,Y88>N(- - -)|c271>t|c287>a,S96>Y(+ - -)|g303>a|c309>t| c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|t111>c|g112>a,A38>N(- - -)|c113>a,A38>N(- - -)| g117>c,M39>I(+ + -)|a145>g,R49>G(- - -)|a157>g,M53>V(+ - -)| t189>c|c192>t| a197>g,K66>R(+ + +)|g210>a|c213>g,F71>L(+ - -)|a235>t,T79>S(+ - +)|c257>t,T86>I(- - -)|a258>t,T86>I(- - -)|t262>a,Y88>N(- - -)|c271>t|c287>a,S96>Y(+ - -)|g303>a|c309>t| 320 222 201 21 7 14 3 4 4 2 0 2 2 1 1 2 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 2 0 1 0 1 0 0 0 1 0 0 51 51 48 3 0 3 2 0 0 0 0 0 0 0 1 0 0 0 30 24 22 2 2 0 0 0 1 1 0 0 0 0 0 0 0 0 117 114 103 11 4 7 1 2 3 0 0 1 2 1 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|ta,262-263(FR3)|aa,290-291(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,11-12(FR1)|tt,62-63(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,120-121(FR2)|tt,149-150(FR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,201-202(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacagcttcactaactacaat atccattgggtgcgccaggcccccggacaagggcttgagtgggtgggatgg atcaacgctggcaatggcaataca agatattcacagaaattgcagggcagagtcaccatttccagggacacatccgcgagcattgccaacatggagttgagcagcctgagatatgaagacacggctgtatattattgt gcgagagacggctataggagtggcaacggggctgactat ARDGYRSGNGADY IGHV1-3 IGHD6-25 IGHJ4 ggatacagcttcactaactacaat atccattgggtgcgccaggcccccggacaagggcttgagtgggtgggatgg atcaacgctggcaatggcaataca agatattcacagaaattgcagggcagagtcaccatttccagggacacatccgcgagcattgccaacatggagttgagcagcctgagatatgaagacacggctgtatattattgt gcgagagacggctataggagtggcaacggggctgactat IGA2 21 213 13 8 8 13 5 8 4 3 10 4 JY8QFUQ01AWIJ1 14771 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 782 85.12 183/215 nt NA Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F 141 77.08 37/48 nt Homsap IGHD3-10*01 F 1 8 7 13 8.7.13 [1.17.38.11] CATVRHLGERIFDFW in-frame + tctctggggccaccatgagtagtttctactggagctgggttcggcagtccccagggaggggactggagtggattggatttgtttcttacagtgggcccaccaactacagcccctccctcaagagtcgagtcaacttatcactggacgcggccaacaaacagttctctttgcagctgcgttctgtgaccgctgcggacacggccatttattactgtgcgacagtacgacatctcggggaacgaatattcgatttctggggccagggtatcctggtcaccgtcccctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [285] t81>g|g83>c,G28>A(- + -)|t85>a,S29>T(+ - +)|c90>g,I30>M(+ + -)|a110>t,Y37>F(- + -)|a124>g,I42>V(+ - +)|c126>t,I42>V(+ - +)|c133>t,P45>S(+ - -)|a143>g,K48>R(+ + +)|g162>a|a164>t,Y55>F(- + -)|a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|a170>c,Y57>S(+ - -)|a190>c,S64>P(+ - -)|g191>c,S64>P(+ - -)|a203>g,N68>S(- - -)|c230>a,T77>N(- + -)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|a244>g,T82>A(- - -)|t247>g,S83>A(- + -)|g252>c,K84>N(+ - -)|c255>a,N85>K(+ - -)|c264>t|c265>t|a268>c,K90>Q(+ - -)|a274>c,S92>R(- - -)|c276>t,S92>R(- - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|g317>c,R106>T(- - -)| t81>g|g83>c,G28>A(- + -)|t85>a,S29>T(+ - +)|c90>g,I30>M(+ + -)|a110>t,Y37>F(- + -)| a124>g,I42>V(+ - +)|c126>t,I42>V(+ - +)|c133>t,P45>S(+ - -)|a143>g,K48>R(+ + +)|g162>a|a164>t,Y55>F(- + -)| a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|a170>c,Y57>S(+ - -)|a190>c,S64>P(+ - -)|g191>c,S64>P(+ - -)| a203>g,N68>S(- - -)|c230>a,T77>N(- + -)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|a244>g,T82>A(- - -)|t247>g,S83>A(- + -)|g252>c,K84>N(+ - -)|c255>a,N85>K(+ - -)|c264>t|c265>t|a268>c,K90>Q(+ - -)|a274>c,S92>R(- - -)|c276>t,S92>R(- - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| g317>c,R106>T(- - -)|a320>t| 319 (320) 222 (223) 189 33 (34) 4 (5) 29 6 2 6 0 4 2 3 (4) 1 5 1 1 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 0 0 0 0 0 1 1 1 1 0 1 51 51 45 6 1 5 2 1 2 0 0 0 1 0 0 0 0 0 30 21 16 5 0 5 1 0 1 0 2 0 0 0 1 0 0 0 117 114 98 16 2 14 3 1 3 0 2 2 1 0 2 0 1 1 7 (8) 7 6 1 (2) 0 (1) 1 0 0 0 0 0 0 0 (1) 0 1 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggggccaccatgagtagtttctac tggagctgggttcggcagtccccagggaggggactggagtggattggattt gtttcttacagtgggcccacc aactacagcccctccctcaagagtcgagtcaacttatcactggacgcggccaacaaacagttctctttgcagctgcgttctgtgaccgctgcggacacggccatttattactgt gcgacagtacgacatctcggggaacgaatattcgatttc ATVRHLGERIFDF IGHV4-59 IGHD3-10 IGHJ4 ggggccaccatgagtagtttctac tggagctgggttcggcagtccccagggaggggactggagtggattggattt gtttcttacagtgggcccacc aactacagcccctccctcaagagtcgagtcaacttatcactggacgcggccaacaaacagttctctttgcagctgcgttctgtgaccgctgcggacacggccatttattactgt gcgacagtacgacatctcggggaacgaatattcgatttc IGA1 32 210 14 18 8 16 6 16 3 1 19 9 JY8QFUQ01AWLNY 16216 productive (see comment) Homsap IGHV6-1*01 F, or Homsap IGHV6-1*02 F 1034 93.99 219/233 nt 92.7 216/233 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD1-14*01 ORF 3 10 9 19 10.9.19 [3.17.38.11] CARASRRDRQSDRNYYELDFW in-frame - Potentially, because of detected insertion/deletion in FR1-IMGT at codon 26 of V-REGION: 'C' (1 nucleotides at position 9 to 9 in the user submitted sequence), (cause frameshift);in CDR1-IMGT at codon 38 of V-REGION: 'A' (1 nucleotides at position 42 to 42 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 55 of V-REGION: 'G' (1 nucleotides at position 93 to 93 in the user submitted sequence), (cause frameshift); gtgccatcCtccggggacagtgtctctagcaacagtgccacAttggaactggatcaggcagtccccaacgggaggccttgagtggctgggaaGggacatcctacaggtccaaatggtatagtgattatgcggtgtctgtgaaaagtcgaataaccatcaacccagacacatccaagaaccagttctccctgcaattgaactccgttagtcccgaggacacggctgtgtattactgtgcaagagcttccagacgcgatcgtcagtctgatcggaactactatgagctggacttctggggccagggaatcttggtcgccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [324] t111>c|g112>a,A38>T(- - -)|t139>a,S47>T(+ - +)|a142>g,R48>G(- - -)|a170>c,Y57>S(+ - -)|g186>a|a194>g,N65>S(- - -)|a204>g|a207>g|g270>a|c271>t|t279>c|g282>t|c284>g,T95>S(+ - +)| t111>c|g112>a,A38>T(- - -)| t139>a,S47>T(+ - +)|a142>g,R48>G(- - -)| a170>c,Y57>S(+ - -)|g186>a|a194>g,N65>S(- - -)| a204>g|a207>g|g270>a|c271>t|t279>c|g282>t|c284>g,T95>S(+ - +)| a320>c| 319 (320) 240 (241) 226 14 (15) 8 (9) 6 4 3 1 2 1 (2) 0 0 1 0 1 1 0 78 11 11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 1 1 0 1 0 1 0 0 0 0 0 0 0 0 51 51 49 2 0 2 1 0 0 0 0 0 0 1 0 0 0 0 30 27 24 3 1 2 1 1 0 0 1 0 0 0 0 0 0 0 117 114 107 7 6 1 2 1 1 1 0 0 0 0 0 1 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,5-6(FR1)|aa,40-41(FR1)|aa,103-104(CDR1)|aa,118-119(FR2)|aa,162-163(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,184-185(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|ta,206-207(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,227-228(FR3)|aa,235-236(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,5-6(FR1)|tt,114-115(CDR1)|tt,149-150(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|tt,198-199(FR3)|ta,206-207(FR3)|ta,227-228(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggta,3-6(FR1)|agct,8-11(FR1)|agca,14-17(FR1)|agca,100-103(CDR1)|ggca,128-131(FR2)|ggct,155-158(FR2)|ggta,188-191(CDR2)|agta,204-207(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tgcc,69-72(FR1)|tgct,108-111(CDR1)|aact,118-121(FR2)|tact,169-172(CDR2)|aacc,228-231(FR3)|aacc,235-238(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|aact,274-277(FR3)|tact,307-310(FR3)| gtgccatctcc ggggacagtgtctctagcaacagtgccact tggaactggatcaggcagtccccaacgggaggccttgagtggctgggaagg acatcctacaggtccaaatggtatagt gattatgcggtgtctgtgaaaagtcgaataaccatcaacccagacacatccaagaaccagttctccctgcaattgaactccgttagtcccgaggacacggctgtgtattactgt gcaagagcttccagacgcgatcgtcagtctgatcggaactactatgagctggacttc ARASRRDRQSDRNYYELDF IGHV6-1 IGHD1-14 IGHJ4 ggggacagtgtctctagcaacagtgccact tggaactggatcaggcagtccccaacgggaggccttgagtggctgggaagg acatcctacaggtccaaatggtatagt gattatgcggtgtctgtgaaaagtcgaataaccatcaacccagacacatccaagaaccagttctccctgcaattgaactccgttagtcccgaggacacggctgtgtattactgt gcaagagcttccagacgcgatcgtcagtctgatcggaactactatgagctggacttc IGA2 14 222 10 4 4 6 6 8 6 2 3 3 JY8QFUQ01AWOLO 17678 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 940 93.06 201/216 nt 92.59 200/216 nt Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD3-16*01 F 2 8 8 16 8.8.16 [1.17.37.11] CARRLNNYDSGEYAMDVW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 97 of V-REGION: 1 nucleotides (from position 194 in the user submitted sequence), (cause frameshift) ttctggatacacgtttatgaattactggatcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatcatctatcctggtacctctgagaccagatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgccttcgtgcagtggagcagcctgaaggcccggacaccgccatatattactgtgcgaggcgactcaataattacgactcgggagagtacgctatggacgtctggggccaagggaccacggtcaccgtctcctcaacctccaccaagggcccatcggtcttcccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [297, 296] g86>c,S29>T(+ - +)|c87>g,S29>T(+ - +)|c104>t,T35>M(- - -)|c105>g,T35>M(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g184>a,D62>T(- + -)|a185>c,D62>T(- + -)|t192>g,D64>E(+ - +)|c213>t|c234>t|t251>c,I84>T(- - -)|a263>t,Y88>F(- + -)|c265>g,L89>V(+ - +)|g303>a,M101>I(+ + -)| g86>c,S29>T(+ - +)|c87>g,S29>T(+ - +)|c104>t,T35>M(- - -)|c105>g,T35>M(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g184>a,D62>T(- + -)|a185>c,D62>T(- + -)|t192>g,D64>E(+ - +)| c213>t|c234>t|t251>c,I84>T(- - -)|a263>t,Y88>F(- + -)|c265>g,L89>V(+ - +)|g303>a,M101>I(+ + -)| a318>g|a320>g| 317 (320) 221 (224) 206 (207) 15 (17) 2 (4) 13 0 (2) 3 4 1 1 0 1 0 1 3 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 1 2 0 0 0 0 0 1 2 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 1 0 0 1 0 0 0 0 0 0 1 117 113 107 6 2 4 0 1 2 1 0 0 1 0 0 1 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ttct ggatacacgtttatgaattactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtacctctgagacc agatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgccttcgtgcagtggagcagcctgaaggcccggacaccgccatatattactgt gcgaggcgactcaataattacgactcgggagagtacgctatggacgtc ARRLNNYDSGEYAMDV IGHV5-51 IGHD3-16 IGHJ6 ggatacacgtttatgaattactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtacctctgagacc agatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgccttcgtgcagtggagcagcctgaaggcccggacaccgccatatattactgt gcgaggcgactcaataattacgactcgggagagtacgctatggacgtc IGG1 15 212 8 7 7 11 1 4 2 0 4 9 JY8QFUQ01AWOO7 21110 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 851 88.07 192/218 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-3*01 F 3 8 8 10 8.8.10 [1.17.38.11] CASFRVLESGYW in-frame + tctctggattcacattcggtagttttatgatgaactgggtccgccaggctccagggaagggactggagtgggtcgcatcgattagccctactagtactttcatagactacgcagactcagtgaggggccggttcaccatctccagagataacgccgagaacttactgtatctgcaaatgaacggcctgagagtcgaagacacggctgtctattactgtgcgagttttcgtgtcctggaaagtggctactggggccagggaaccttggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [279] c74>t|c87>a|a103>g,S35>G(+ + -)|c108>t|a110>t,Y37>F(- + -)|g113>t,S38>M(- - -)|c114>g,S38>M(- - -)|g147>a|t160>g,S54>A(- + -)|c165>g|t171>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g176>c,S59>T(+ - +)|g188>c,S63>T(+ - +)|a191>t,Y64>F(- + -)|t196>g,Y66>D(- - -)|a215>g,K72>R(+ + +)|a225>g|c243>t|a250>g,K84>E(+ - -)|c257>t,S86>L(- - -)|a277>g,S93>G(+ + -)|c287>t,A96>V(+ - +)|g291>a|g303>c| c74>t| c87>a|a103>g,S35>G(+ + -)|c108>t|a110>t,Y37>F(- + -)|g113>t,S38>M(- - -)|c114>g,S38>M(- - -)| g147>a|t160>g,S54>A(- + -)|c165>g| t171>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g176>c,S59>T(+ - +)|g188>c,S63>T(+ - +)|a191>t,Y64>F(- + -)| t196>g,Y66>D(- - -)|a215>g,K72>R(+ + +)|a225>g|c243>t|a250>g,K84>E(+ - -)|c257>t,S86>L(- - -)|a277>g,S93>G(+ + -)|c287>t,A96>V(+ - +)|g291>a|g303>c| a318>t,R106>S(- - -)|g319>t|a320>t| 317 (320) 223 (226) 197 26 (29) 10 (12) 16 (17) 5 2 5 1 1 1 2 (4) 0 4 2 1 (2) 2 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 18 6 2 4 1 0 1 0 0 1 1 0 0 1 1 0 51 51 48 3 2 1 0 1 0 0 0 0 0 0 0 1 0 1 30 24 18 6 1 5 0 0 0 1 1 0 1 0 3 0 0 0 117 114 104 10 4 6 4 1 3 0 0 0 0 0 1 0 0 1 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 0 0 0 0 0 0 (2) 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tctct ggattcacattcggtagttttatg atgaactgggtccgccaggctccagggaagggactggagtgggtcgcatcg attagccctactagtactttcata gactacgcagactcagtgaggggccggttcaccatctccagagataacgccgagaacttactgtatctgcaaatgaacggcctgagagtcgaagacacggctgtctattactgt gcgagttttcgtgtcctggaaagtggctac ASFRVLESGY IGHV3-21 IGHD3-3 IGHJ4 ggattcacattcggtagttttatg atgaactgggtccgccaggctccagggaagggactggagtgggtcgcatcg attagccctactagtactttcata gactacgcagactcagtgaggggccggttcaccatctccagagataacgccgagaacttactgtatctgcaaatgaacggcctgagagtcgaagacacggctgtctattactgt gcgagttttcgtgtcctggaaagtggctac IGA2 25 213 12 13 6 14 6 11 6 3 7 9 JY8QFUQ01AWPED 11875 productive Homsap IGHV3-7*01 F 927 92.17 200/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-3*02 F 2 8 8 14 8.8.14 [1.17.38.11] CARGSYWHYDTFGDYW in-frame + ctctggatttacttttaacaactattggatgacctgggttcgccaggctccagggaaggggctggaatgggtggccaacataaagcaacatggaggtgagacgtactatgcggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgtgcgagagggtcgtattggcattatgataccttcggcgactattggggcccgggagtcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 100 100 [291] c84>t|c87>t|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|c126>t|g153>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)|t203>c,V68>A(+ - +)|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| c84>t|c87>t|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|c126>t|g153>a| g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)| t203>c,V68>A(+ - +)|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| a320>g| 319 (320) 224 (225) 207 17 (18) 5 (6) 12 3 (4) 3 3 2 2 0 0 0 3 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 2 2 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 0 1 0 0 0 30 24 20 4 0 4 2 0 0 0 1 0 0 0 1 0 0 0 117 114 109 5 1 4 1 0 0 1 1 0 0 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatttacttttaacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccaac ataaagcaacatggaggtgagacg tactatgcggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat ARGSYWHYDTFGDY IGHV3-7 IGHD3-3 IGHJ4 ggatttacttttaacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccaac ataaagcaacatggaggtgagacg tactatgcggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat IGG1 17 213 11 6 6 10 5 7 3 2 5 7 JY8QFUQ01AWSX6 11866 productive Homsap IGHV3-48*01 F 981 94.93 206/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 3 8 8 13 8.8.13 [1.17.38.11] CARERGNSYGEIDYW in-frame + ctctggtttcaccttcaggagctctggcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagcagtagtactactaccaaatactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacatggctgtgtattactgtgcgagagaacgtggaaacagctatggagagattgactactggggccagggaaccctggtcaccgtctcctcagcttccaccaagggccccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG4 100 81 [289] a81>t|t105>g,S35>R(- - -)|a110>c,Y37>S(+ - -)|a112>g,S38>G(+ + -)|t171>c|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t194>a,I65>K(- + -)|a200>g,Y67>C(- - -)|a254>g,N85>S(- - -)|c296>t,T99>M(- - -)| a81>t|t105>g,S35>R(- - -)|a110>c,Y37>S(+ - -)|a112>g,S38>G(+ + -)| t171>c|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t194>a,I65>K(- + -)| a200>g,Y67>C(- - -)|a254>g,N85>S(- - -)|c296>t,T99>M(- - -)| 320 225 214 11 2 9 3 0 1 1 1 0 1 1 2 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 0 0 1 0 1 0 0 0 0 1 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 0 0 0 1 0 0 0 1 2 0 0 0 117 114 111 3 0 3 2 0 1 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggtttcaccttcaggagctctggc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagcagtagtactactaccaaa tactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacatggctgtgtattactgt gcgagagaacgtggaaacagctatggagagattgactac ARERGNSYGEIDY IGHV3-48 IGHD5-18 IGHJ4 ggtttcaccttcaggagctctggc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagcagtagtactactaccaaa tactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacatggctgtgtattactgt gcgagagaacgtggaaacagctatggagagattgactac IGG4 11 213 5 6 1 3 4 8 0 2 3 6 JY8QFUQ01AX0TL 18408 productive (see comment) Homsap IGHV4-61*02 F 892 89.95 197/219 nt 88.58 194/219 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-19*01 F 1 10 7 13 10.7.13 [1.16.38.11] CARQGDSSGPWNDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 39 of V-REGION: 'G' (1 nucleotides at position 36 to 36 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 64 of V-REGION: 'A' (1 nucleotides at position 103 to 103 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 50 of V-REGION: 1 nucleotides (from position 70 in the user submitted sequence), (cause frameshift) ctccggtggctccatcagtactggttattactactGggagctggatccggcagtccgccgggaagggactgaatggattgggcgcatgtctgccagagggggAcagcaactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgtgcgagacaaggggatagtagtggaccctggaatgactactggggccagggaatcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [292] t78>c|c93>t|g101>c,S34>T(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c133>t,P45>S(+ - -)|g153>a|t165>c|c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>G(+ + -)|c194>g,T65>S(+ - +)|a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| t78>c| c93>t|g101>c,S34>T(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)| c133>t,P45>S(+ - -)|g153>a|t165>c| c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>G(+ + -)|c194>g,T65>S(+ - +)| a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g319>c| 318 (320) 225 (227) 203 (204) 22 (23) 5 (6) 17 3 3 3 2 3 0 2 1 2 (3) 3 0 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 30 26 4 1 3 0 1 1 0 0 0 1 0 1 0 0 0 51 50 47 3 2 1 0 1 1 1 0 0 0 0 0 0 0 0 30 21 15 6 0 6 2 0 0 0 1 0 0 1 0 2 0 0 117 114 106 8 1 7 1 1 1 0 2 0 1 0 1 1 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctcc ggtggctccatcagtactggttattactac tggagctggatccggcagtccgccgggaagggactgaatggattgggcgc atgtctgccagagggggcagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac ARQGDSSGPWNDY IGHV4-61 IGHD6-19 IGHJ4 ggtggctccatcagtactggttattactac tggagctggatccggcagtccgccgggaagggactgaatggattgggcgc atgtctgccagagggggcagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac IGG2 21 215 10 11 6 11 4 10 3 1 8 9 JY8QFUQ01AX3JG 10369 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 941 92.66 202/218 nt NA Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 241 98 49/50 nt Homsap IGHD6-19*01 F 2 8 8 14 8.8.14 [1.17.38.11] CAKRLQAVADDAFDFW in-frame - cctctggattcatctttagtagctatgccatgggctgggtccgccaggctccagggaaggggctggagtgggtctcaactattagtgccagtggtaagagcacatactacgcagactccgtgaagggccggttcagcatctccagagacaattccaagaacacgatgtctgtgcaaatgagcagcctgagagccgaagacacggccatatattactgtgcgaaaaggttgcaagcagtggctgatgatgcttttgatttctggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [291] c86>t,T29>I(- - -)|c105>t|a118>g,S40>G(+ + -)|g163>a,A55>T(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g187>a,G63>K(- - -)|g188>a,G63>K(- - -)|t189>g,G63>K(- - -)|c230>g,T77>S(+ - +)|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|c265>g,L89>V(+ - +)|a275>g,N92>S(- - -)|g291>a|g301>a,V101>I(+ - +)| c86>t,T29>I(- - -)|c105>t| a118>g,S40>G(+ + -)|g163>a,A55>T(- - -)| g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g187>a,G63>K(- - -)|g188>a,G63>K(- - -)|t189>g,G63>K(- - -)| c230>g,T77>S(+ - +)|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|c265>g,L89>V(+ - +)|a275>g,N92>S(- - -)|g291>a|g301>a,V101>I(+ - +)| g319>a|a320>g| 318 (320) 224 (226) 208 16 (18) 2 (4) 14 2 (3) 5 (6) 2 1 1 1 0 0 1 2 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 2 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 30 24 19 5 0 5 0 2 0 1 0 0 0 0 1 0 0 1 117 114 107 7 1 6 1 2 0 0 1 1 0 0 0 2 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcatctttagtagctatgcc atgggctgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtgccagtggtaagagcaca tactacgcagactccgtgaagggccggttcagcatctccagagacaattccaagaacacgatgtctgtgcaaatgagcagcctgagagccgaagacacggccatatattactgt gcgaaaaggttgcaagcagtggctgatgatgcttttgatttc AKRLQAVADDAFDF IGHV3-23 IGHD6-19 IGHJ3 ggattcatctttagtagctatgcc atgggctgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtgccagtggtaagagcaca tactacgcagactccgtgaagggccggttcagcatctccagagacaattccaagaacacgatgtctgtgcaaatgagcagcctgagagccgaagacacggccatatattactgt gcgaaaaggttgcaagcagtggctgatgatgcttttgatttc IGA1 16 213 10 6 7 11 3 5 1 1 8 6 JY8QFUQ01AX7BT 9901 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 818 86.98 187/215 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-21*02 F 3 8 7 15 8.7.15 [1.17.38.11] CARGRTVVTVIDYIDYW in-frame + tctctggggactccattagtggttactattggacgtggatccggcagaccccagggaagggactggagtggattggaaatgtccattacactgggagtaccaagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgtgcgagaggacgaacggtggtgactgttattgactacattgactattggggtcagggagccctggtcagcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [291] t81>g|g83>a,G28>D(- - -)|c90>t|a106>g,S36>G(+ + -)|c114>t|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>a,P45>T(+ + -)|g162>a|t163>a,Y55>N(- - -)|a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|c192>t|c198>g,N66>K(+ - -)|a203>g,N68>S(- - -)|a234>g,I78>M(+ + -)|a240>t|c245>t,T82>M(- - -)|a251>g,K84>R(+ + +)|t262>a,S88>T(+ - +)|g267>c|g270>a|c271>t|g275>c,S92>T(+ - +)|g282>a|a283>g,T95>A(- - -)|g303>c| t81>g|g83>a,G28>D(- - -)|c90>t|a106>g,S36>G(+ + -)|c114>t| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>a,P45>T(+ + -)|g162>a|t163>a,Y55>N(- - -)| a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|c192>t| c198>g,N66>K(+ - -)|a203>g,N68>S(- - -)|a234>g,I78>M(+ + -)|a240>t|c245>t,T82>M(- - -)|a251>g,K84>R(+ + +)|t262>a,S88>T(+ - +)|g267>c|g270>a|c271>t|g275>c,S92>T(+ - +)|g282>a|a283>g,T95>A(- - -)|g303>c| a320>g| 319 (320) 222 (223) 194 28 (29) 11 (12) 17 6 (7) 4 5 1 0 1 1 2 5 2 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 3 2 1 1 2 0 0 0 0 0 0 0 0 1 51 51 46 5 1 4 0 1 0 0 0 1 0 1 1 1 0 0 30 21 17 4 1 3 1 0 1 1 0 0 0 0 1 0 0 0 117 114 100 14 6 8 4 2 2 0 0 0 1 1 3 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggggactccattagtggttactat tggacgtggatccggcagaccccagggaagggactggagtggattggaaat gtccattacactgggagtacc aagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttattgactacattgactat ARGRTVVTVIDYIDY IGHV4-59 IGHD2-21 IGHJ4 ggggactccattagtggttactat tggacgtggatccggcagaccccagggaagggactggagtggattggaaat gtccattacactgggagtacc aagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttattgactacattgactat IGA1 28 210 16 12 9 17 7 11 7 4 12 5 JY8QFUQ01AX7OK 4823 productive Homsap IGHV3-7*01 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F 167 84.78 39/46 nt Homsap IGHD7-27*01 F 1 8 8 6 8.8.6 [1.17.38.11] CSRLLEEW in-frame + ctctggtttcacctttagtaacgattggatggactgggtccgccaggctccagggaaggggctggagtgggtggccaatataaagggagatggaagtgagaaaaactatgtagactctgcgaagggccgattcatcatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgttcgagactccttgaggaatggggccagggaaccctggtcatcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaaagatgggaacgtggtcgtcgcatgcctggtcccagggcttcttcccccg NA IGA2 71 95 [266, 268] a81>t|g107>a,S36>N(- - -)|t109>g,Y37>D(- - -)|a118>g,S40>D(- - -)|g119>a,S40>D(- - -)|c165>t|c172>g,Q58>G(- - -)|a173>g,Q58>G(- - -)|t196>a,Y66>N(- - -)|g204>a|t212>c,V71>A(+ - +)|c230>t,T77>I(- - -)|c287>t,A96>V(+ - +)|g313>t,A105>S(- + -)| a81>t|g107>a,S36>N(- - -)|t109>g,Y37>D(- - -)| a118>g,S40>D(- - -)|g119>a,S40>D(- - -)|c165>t| c172>g,Q58>G(- - -)|a173>g,Q58>G(- - -)| t196>a,Y66>N(- - -)|g204>a|t212>c,V71>A(+ - +)|c230>t,T77>I(- - -)|c287>t,A96>V(+ - +)| g313>t,A105>S(- + -)|g319>c|a320>t| 318 (320) 223 (225) 209 14 (16) 3 (5) 11 2 3 3 1 0 0 1 (2) 1 0 (1) 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 0 0 0 0 1 0 0 0 0 1 51 51 48 3 1 2 1 1 1 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 1 0 0 117 114 109 5 1 4 0 1 2 1 0 0 0 1 0 0 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 0 0 0 0 0 0 (1) 0 0 (1) 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggtttcacctttagtaacgattgg atggactgggtccgccaggctccagggaaggggctggagtgggtggccaat ataaagggagatggaagtgagaaa aactatgtagactctgcgaagggccgattcatcatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgt tcgagactccttgaggaa SRLLEE IGHV3-7 IGHD7-27 IGHJ4 ggtttcacctttagtaacgattgg atggactgggtccgccaggctccagggaaggggctggagtgggtggccaat ataaagggagatggaagtgagaaa aactatgtagactctgcgaagggccgattcatcatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgt tcgagactccttgaggaa IGA2 13 213 9 4 6 7 3 6 2 1 6 4 JY8QFUQ01AXASL 18244 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 907 92.02 196/213 nt 91.55 195/213 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 3 8 7 12 8.7.12 [0.17.38.11] CARDTGQWKDFDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 58 of V-REGION: 1 nucleotides (from position 84 in the user submitted sequence), (cause frameshift) tggattcagcttcagtacctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtactaatacggggggactgacacagcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacggtgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattattgtgcaagagatacggggcagtggaaggactttgactactggggccagggaaccctggtcaccgtctccccagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [280] c86>g,T29>S(+ - +)|g107>c,S36>T(+ - +)|t167>c,I56>T(- - -)|t174>c|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|c259>g,L87>V(+ - +)|g303>t|c309>t| c86>g,T29>S(+ - +)|g107>c,S36>T(+ - +)| t167>c,I56>T(- - -)|t174>c|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|c259>g,L87>V(+ - +)|g303>t|c309>t| 320 221 204 17 4 13 3 1 1 2 1 0 0 0 3 4 1 1 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 0 0 1 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 23 16 7 1 6 2 1 0 2 0 0 0 0 1 0 0 1 117 114 106 8 3 5 1 0 1 0 1 0 0 0 1 3 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t ggattcagcttcagtacctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt actaatacggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacggtgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattattgt gcaagagatacggggcagtggaaggactttgactac ARDTGQWKDFDY IGHV3-74 IGHD6-19 IGHJ4 ggattcagcttcagtacctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt actaatacggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacggtgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattattgt gcaagagatacggggcagtggaaggactttgactac IGA1 17 212 7 10 2 10 5 7 3 1 5 8 JY8QFUQ01AXCGR 4532 productive Homsap IGHV3-7*01 F 977 94.5 206/218 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD4-17*01 F 3 8 8 11 8.8.11 [1.17.38.11] CARTSSREITDYW in-frame - cctctggattcacctttggtagctattggatggcctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagcaagatggaagtggtacatactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaattcactgtttctgcaaatggacagcctgagagtcgaggacacggctctgtattactgtgcgagaacctcgtcgcgggagattacggattactggggccagggaaccctggtcaccgtttcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [282, 281] a103>g,S35>G(+ + -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a191>g,E64>G(- - -)|g192>t,E64>G(- - -)|a194>c,K65>T(- - -)|a232>g,I78>V(+ - +)|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)| a103>g,S35>G(+ + -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)| a191>g,E64>G(- - -)|g192>t,E64>G(- - -)|a194>c,K65>T(- - -)| a232>g,I78>V(+ - +)|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)| g319>a|a320>c| 318 (320) 224 (226) 212 12 (14) 1 (3) 11 5 0 (1) 2 0 1 (2) 0 1 0 2 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 30 24 21 3 0 3 1 0 0 0 1 0 0 0 0 0 1 0 117 114 108 6 1 5 2 0 2 0 0 0 1 0 1 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttggtagctattgg atggcctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaagtggtaca tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaattcactgtttctgcaaatggacagcctgagagtcgaggacacggctctgtattactgt gcgagaacctcgtcgcgggagattacggattac ARTSSREITDY IGHV3-7 IGHD4-17 IGHJ4 ggattcacctttggtagctattgg atggcctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaagtggtaca tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaattcactgtttctgcaaatggacagcctgagagtcgaggacacggctctgtattactgt gcgagaacctcgtcgcgggagattacggattac IGA2 12 213 7 5 2 5 5 7 1 0 7 4 JY8QFUQ01AXE56 13059 productive (see comment) Homsap IGHV3-9*01 F (see comment) 778 84.72 183/216 nt 84.26 182/216 nt Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD3-10*01 F 3 8 8 16 8.8.16 [1.17.37.11] CVKDMVAGQGLLSRFDHW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (84,72% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR3-IMGT, from codon 68 of V-REGION: 1 nucleotides (from position 111 in the user submitted sequence), (cause frameshift) ctctggattcgactttaaggaatatgccatacactgggtccggcaagttccaggaaagggcctggagtgggtcgcgggcatcaactggaatcggggcaaagcattgtatgggactctgtgaggggccgattcaccatctccagagacaacgcccagaactccgtgtctctgcaaatgaacagtctgaggcctgacgacacggccttgtatatctgtgtaaaagacatggtcgcgggtcagggactcttgtcacggtttgaccactggggccagggagtcctggtcaccgtctctccagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [296] a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|g103>a,D35>K(+ - -)|t105>g,D35>K(+ - -)|t108>a,D36>E(+ - +)|g117>a,M39>I(+ + -)|c134>t,A45>V(+ - +)|g141>a|t160>g,S54>A(- + -)|a162>g,S54>A(- + -)|t165>c|t168>c|g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|a184>c,S62>R(- - -)|t186>g,S62>R(- - -)|t189>c|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|a193>g,I65>A(+ - +)|t194>c,I65>A(+ - +)|g196>t,G66>L(- - -)|g197>t,G66>L(- - -)|c198>g,G66>L(- - -)|a215>g,K72>R(+ + +)|a250>c,K84>Q(+ - -)|c259>g,L87>V(+ - +)|a263>c,Y88>S(+ - -)|a285>g|g286>c,A96>P(- - -)|g291>c,E97>D(+ - +)|t307>a,Y103>I(- - -)|a308>t,Y103>I(- - -)|c314>t,A105>V(+ - +)| a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|g103>a,D35>K(+ - -)|t105>g,D35>K(+ - -)|t108>a,D36>E(+ - +)| g117>a,M39>I(+ + -)|c134>t,A45>V(+ - +)|g141>a|t160>g,S54>A(- + -)|a162>g,S54>A(- + -)|t165>c| t168>c|g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|a184>c,S62>R(- - -)|t186>g,S62>R(- - -)|t189>c|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|a193>g,I65>A(+ - +)|t194>c,I65>A(+ - +)| g196>t,G66>L(- - -)|g197>t,G66>L(- - -)|c198>g,G66>L(- - -)|a215>g,K72>R(+ + +)|a250>c,K84>Q(+ - -)|c259>g,L87>V(+ - +)|a263>c,Y88>S(+ - -)|a285>g|g286>c,A96>P(- - -)|g291>c,E97>D(+ - +)|t307>a,Y103>I(- - -)|a308>t,Y103>I(- - -)| c314>t,A105>V(+ - +)|t321>c| 320 (322) 224 (226) 190 (191) 34 (35) 5 (6) 29 5 5 2 5 (6) 3 2 1 2 2 2 2 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 1 0 0 0 1 0 1 0 0 0 1 51 51 45 6 2 4 1 2 1 1 0 0 0 0 0 0 0 1 30 24 14 10 2 8 1 2 0 4 1 1 0 0 0 0 0 1 117 113 101 12 1 11 2 0 0 0 2 0 1 1 2 2 2 0 8 (10) 8 7 (8) 1 (2) 0 (1) 1 0 0 1 0 (1) 0 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcgactttaaggaatatgcc atacactgggtccggcaagttccaggaaagggcctggagtgggtcgcgggc atcaactggaatcggggcaaagca ttgtatgggactctgtgaggggccgattcaccatctccagagacaacgcccagaactccgtgtctctgcaaatgaacagtctgaggcctgacgacacggccttgtatatctgt gtaaaagacatggtcgcgggtcagggactcttgtcacggtttgaccac VKDMVAGQGLLSRFDH IGHV3-9 IGHD3-10 IGHJ4 ggattcgactttaaggaatatgcc atacactgggtccggcaagttccaggaaagggcctggagtgggtcgcgggc atcaactggaatcggggcaaagca ttgtatgggactctgtgaggggccgattcaccatctccagagacaacgcccagaactccgtgtctctgcaaatgaacagtctgaggcctgacgacacggccttgtatatctgt gtaaaagacatggtcgcgggtcagggactcttgtcacggtttgaccac IGG1 33 212 16 17 6 14 10 19 3 2 15 13 JY8QFUQ01AXNWU 22006 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 1009 95.89 210/219 nt 94.52 207/219 nt Homsap IGHJ4*02 F (see comment) 222 95.83 46/48 nt Homsap IGHD3-22*01 F 2 8 8 18 8.8.18 [2.17.38.11] CAKDKWYYDSSGYQYYLDSW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT at codon 29 of V-REGION: 'C' (1 nucleotides at position 13 to 13 in the user submitted sequence), (cause frameshift);in CDR1-IMGT at codon 37 of V-REGION: 'G' (1 nucleotides at position 27 to 27 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 40 of V-REGION: 'A' (1 nucleotides at position 37 to 37 in the user submitted sequence), (cause frameshift); gcctctggattcCccctttagcaactGatgacatgaAattgggtccgccaggctccagggaaggggctggagtgggtctccggtattagtggtagtggcggtagcacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctggatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaaagacaaatggtactatgatagtagtggttatcaatactaccttgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [307] a85>c,T29>P(+ + -)|g107>a,S36>N(- - -)|c113>a,A38>D(- - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a162>c|c164>g,A55>G(- + -)|t186>c|t262>g,Y88>D(- - -)| a85>c,T29>P(+ + -)|g107>a,S36>N(- - -)|c113>a,A38>D(- - -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a162>c|c164>g,A55>G(- + -)| t186>c| t262>g,Y88>D(- - -)| 320 227 218 9 2 7 0 2 1 1 2 1 0 0 0 1 0 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 1 1 0 0 0 0 0 0 51 51 47 4 1 3 0 1 1 0 1 0 0 0 0 1 0 0 30 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 113 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcccctttagcaactatgac atgaattgggtccgccaggctccagggaaggggctggagtgggtctccggt attagtggtagtggcggtagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctggatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaagacaaatggtactatgatagtagtggttatcaatactaccttgactcc AKDKWYYDSSGYQYYLDS IGHV3-23 IGHD3-22 IGHJ4 ggattcccctttagcaactatgac atgaattgggtccgccaggctccagggaaggggctggagtgggtctccggt attagtggtagtggcggtagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctggatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaagacaaatggtactatgatagtagtggttatcaatactaccttgactcc IGA1 9 213 4 5 3 5 1 4 1 1 4 3 JY8QFUQ01AXTRO 22396 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 946 92.69 203/219 nt 92.24 202/219 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-2*01 F 3 8 8 11 8.8.11 [2.17.38.11] CARFGPLTRPDYW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 72 of V-REGION: 'C' (1 nucleotides at position 124 to 124 in the user submitted sequence), (cause frameshift); gcctctggattcacctttaccaactatggcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtggtgaaaccacataccacgcagaatccgtgCaggggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgtgcgagattcggaccacttactcgacctgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaaggggcccatcggtcttcccccctggcgcccctgctccaggagcacctccgagagcacagcggccctggggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcagg NA IGG2 86 88 [288, 286] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)|c164>g,A55>G(- + -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>c,S64>T(+ - +)|t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a215>g,K72>R(+ + +)|t246>c|c257>a,T86>N(- + -)|g258>t,T86>N(- + -)|c279>t|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)| c164>g,A55>G(- + -)| g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>c,S64>T(+ - +)| t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a215>g,K72>R(+ + +)|t246>c|c257>a,T86>N(- + -)|g258>t,T86>N(- + -)|c279>t|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| a317>g,K106>R(+ + +)|g319>t|a320>t| 316 (320) 223 (227) 207 (208) 16 (19) 2 (4) 14 (15) 1 (2) 3 1 2 0 2 1 (2) 1 2 2 1 (2) 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 21 3 0 3 0 1 0 0 0 0 0 1 1 0 0 0 117 114 105 9 2 7 1 1 1 2 0 2 1 0 0 0 1 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgaaaccaca taccacgcagaatccgtgaggggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgt gcgagattcggaccacttactcgacctgactac ARFGPLTRPDY IGHV3-23 IGHD2-2 IGHJ4 ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgaaaccaca taccacgcagaatccgtgaggggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgt gcgagattcggaccacttactcgacctgactac IGG2 16 213 7 9 4 11 3 5 2 0 8 6 JY8QFUQ01AXVCI 22387 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 959 93.58 204/218 nt NA Homsap IGHJ2*01 F 247 96.23 51/53 nt Homsap IGHD2-21*01 F 1 8 8 12 8.8.12 [1.17.38.11] CAKVVWSLWYFDLW in-frame + cctctacattcgcctttagcaactatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtgtcagatattagtgggagtggtgttagtacacactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgtgcgaaagtagtctggagcctctggtacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [286] g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)|c159>g|c164>a,A55>D(- - -)|t174>g|g188>t,G63>V(- - -)|c192>t|t196>c,Y66>H(+ - -)|a204>g|a214>c,K72>Q(+ - -)|c249>g|a285>t,R95>S(- - -)| g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)| c159>g|c164>a,A55>D(- - -)| t174>g|g188>t,G63>V(- - -)|c192>t| t196>c,Y66>H(+ - -)|a204>g|a214>c,K72>Q(+ - -)|c249>g|a285>t,R95>S(- - -)| a320>t| 319 (320) 225 (226) 211 14 (15) 5 (6) 9 2 2 1 1 1 1 1 (2) 0 1 2 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 2 0 0 0 0 0 0 1 0 0 0 51 51 49 2 1 1 0 0 0 0 0 1 0 0 0 1 0 0 30 24 21 3 2 1 0 0 1 0 0 0 0 0 0 0 1 1 117 114 109 5 2 3 1 0 0 1 1 0 1 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct acattcgcctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtgtcagat attagtgggagtggtgttagtaca cactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgt gcgaaagtagtctggagcctctggtacttcgatctc AKVVWSLWYFDL IGHV3-23 IGHD2-21 IGHJ2 acattcgcctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtgtcagat attagtgggagtggtgttagtaca cactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgt gcgaaagtagtctggagcctctggtacttcgatctc IGG1 14 213 6 8 3 8 3 6 3 2 4 5 JY8QFUQ01AXXC6 16072 productive Homsap IGHV3-48*02 F, or Homsap IGHV3-48*04 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-18*01 F 3 8 8 13 8.8.13 [1.17.38.11] CARDQRGYSYEMDYW in-frame - ctctggattcaccttcagtgtccatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcataccttagtagtggtagtgataccatatactacgcagactctgtgaggggccggttcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagtggcctgagagacgaggacacggctgtttattactgtgcgagagatcagcgtgggtatagttatgaaatggactactggggacagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [288] a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|t109>c,Y37>H(+ - -)|a166>c,I56>L(+ + +)|a175>g,S59>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a215>g,K72>R(+ + +)|a225>g|t246>c|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|a277>g,S93>G(+ + -)|g303>t| a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|t109>c,Y37>H(+ - -)| a166>c,I56>L(+ + +)|a175>g,S59>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)| a215>g,K72>R(+ + +)|a225>g|t246>c|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|a277>g,S93>G(+ + -)|g303>t| 320 225 211 14 3 11 7 1 1 2 1 0 0 0 0 0 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 0 0 1 0 0 0 0 0 0 1 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 2 1 0 0 1 0 0 0 0 0 0 0 117 114 107 7 3 4 4 0 1 1 0 0 0 0 0 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgtccatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac cttagtagtggtagtgataccata tactacgcagactctgtgaggggccggttcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagtggcctgagagacgaggacacggctgtttattactgt gcgagagatcagcgtgggtatagttatgaaatggactac ARDQRGYSYEMDY IGHV3-48 IGHD5-18 IGHJ4 ggattcaccttcagtgtccatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac cttagtagtggtagtgataccata tactacgcagactctgtgaggggccggttcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagtggcctgagagacgaggacacggctgtttattactgt gcgagagatcagcgtgggtatagttatgaaatggactac IGG1 14 213 11 3 2 4 9 10 3 0 4 7 JY8QFUQ01AY7GI 3086 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 872 89.77 193/215 nt 89.3 192/215 nt Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD3-10*01 F 1 8 7 11 8.7.11 [1.17.38.11] CARVGTFNWFGPW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 62 of V-REGION: 1 nucleotides (from position 93 in the user submitted sequence), (cause frameshift) tctggattcagttttgcagattatggcatgggctgggtccgccaacttccagggaaggggctggaatgggtcggtggtgttaattggaatggggcagcgcaggttatgcagtctctgtggagggccgattcatcatctccagagacaacggcaagaagtccctgtatttgcaaatgaacagtctgagagtcgaggacacggccgtgtattactgtgcgagagtcggcacgttcaactggttcggcccctggggccagggaacgctggtcaccgtctcctctgcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 100 [279] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a104>c,D35>A(- - -)|t105>a,D35>A(- - -)|a118>g,S40>G(+ + -)|g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|g153>a|t160>g,S54>G(+ + -)|c161>g,S54>G(+ + -)|a166>g,I56>V(+ - +)|t189>c|a193>g,T65>A(- - -)|a206>t,D69>V(- - -)|a214>g,K72>E(+ - -)|c230>t,T77>I(- - -)|c248>g,A83>G(- + -)|c255>g,N85>K(+ - -)|c265>t|c287>t,A96>V(+ - +)|t301>g,L101>V(+ - +)|c307>t,H103>Y(+ - -)| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a104>c,D35>A(- - -)|t105>a,D35>A(- - -)| a118>g,S40>G(+ + -)|g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|g153>a|t160>g,S54>G(+ + -)|c161>g,S54>G(+ + -)| a166>g,I56>V(+ - +)|t189>c|a193>g,T65>A(- - -)| a206>t,D69>V(- - -)|a214>g,K72>E(+ - -)|c230>t,T77>I(- - -)|c248>g,A83>G(- + -)|c255>g,N85>K(+ - -)|c265>t|c287>t,A96>V(+ - +)|t301>g,L101>V(+ - +)|c307>t,H103>Y(+ - -)| a320>t| 319 (320) 222 (223) 200 22 (23) 3 (4) 19 4 1 6 1 1 0 1 (2) 1 1 4 0 2 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 1 0 1 0 0 1 0 1 0 0 51 51 45 6 1 5 1 1 1 0 0 0 0 0 1 1 0 1 30 23 20 3 1 2 2 0 0 1 0 0 0 0 0 0 0 0 117 114 105 9 1 8 1 0 4 0 0 0 1 0 0 2 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| tct ggattcagttttgcagattatggc atgggctgggtccgccaacttccagggaaggggctggaatgggtcggtggt gttaattggaatggggcagcgca ggttatgcagtctctgtggagggccgattcatcatctccagagacaacggcaagaagtccctgtatttgcaaatgaacagtctgagagtcgaggacacggccgtgtattactgt gcgagagtcggcacgttcaactggttcggcccc ARVGTFNWFGP IGHV3-20 IGHD3-10 IGHJ5 ggattcagttttgcagattatggc atgggctgggtccgccaacttccagggaaggggctggaatgggtcggtggt gttaattggaatggggcagcgca ggttatgcagtctctgtggagggccgattcatcatctccagagacaacggcaagaagtccctgtatttgcaaatgaacagtctgagagtcgaggacacggccgtgtattactgt gcgagagtcggcacgttcaactggttcggcccc IGA1 22 212 12 10 7 12 5 10 2 1 13 6 JY8QFUQ01AY8MO 11939 productive (see comment) Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F 857 89.62 190/212 nt 89.15 189/212 nt Homsap IGHJ6*02 F (see comment) 157 72.58 45/62 nt Homsap IGHD3-10*01 F 3 8 7 16 8.7.16 [0.17.38.11] CARGHRNPGVWDLGLDVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 45 of V-REGION: 'T' (1 nucleotides at position 46 to 46 in the user submitted sequence), (cause frameshift); acagtgggtccttcagtggttacctctggacctggatccgccagtTcccccgggaaggggctggagtggattggagaaattaattatagtgggagaaccaactacaacccgacccttgagagtcaagtcaccatttctgtagacacgtccaagaaccaattctccctgaagcttacctctgtgaccgccgcggacacggctgtctattactgtgcgagaggtcacagaaatccgggagtttgggacctcggtctggacgtctggggccaggggaccacggtcatcgtctcctctgcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [292] t78>c|g79>a,G27>S(+ + -)|t112>c,Y38>L(- - -)|a113>t,Y38>L(- - -)|g119>c,S40>T(+ - +)|c133>t,P45>S(+ - -)|a138>c|g162>a|c168>t|c172>t,H58>Y(+ - -)|a189>g|c192>a,S64>R(- - -)|t208>a,S70>T(+ - +)|c213>t|a214>g,K72>E(+ - -)|g224>a,R75>Q(+ - -)|a234>t|a237>t|g258>a|g273>t|g275>c,S92>T(+ - +)|g303>c| t78>c| g79>a,G27>S(+ + -)|t112>c,Y38>L(- - -)|a113>t,Y38>L(- - -)| g119>c,S40>T(+ - +)|c133>t,P45>S(+ - -)|a138>c|g162>a| c168>t|c172>t,H58>Y(+ - -)|a189>g|c192>a,S64>R(- - -)| t208>a,S70>T(+ - +)|c213>t|a214>g,K72>E(+ - -)|g224>a,R75>Q(+ - -)|a234>t|a237>t|g258>a|g273>t|g275>c,S92>T(+ - +)|g303>c| 320 220 198 22 11 11 2 4 4 2 1 1 3 1 3 0 1 0 78 2 1 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 1 0 0 1 0 0 0 0 0 51 51 47 4 2 2 0 1 1 0 1 0 0 0 1 0 0 0 30 21 17 4 2 2 1 0 2 0 0 1 0 0 0 0 0 0 117 114 104 10 6 4 1 2 1 0 0 0 2 1 2 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ac agtgggtccttcagtggttacctc tggacctggatccgccagtcccccgggaaggggctggagtggattggagaa attaattatagtgggagaacc aactacaacccgacccttgagagtcaagtcaccatttctgtagacacgtccaagaaccaattctccctgaagcttacctctgtgaccgccgcggacacggctgtctattactgt gcgagaggtcacagaaatccgggagtttgggacctcggtctggacgtc ARGHRNPGVWDLGLDV IGHV4-34 IGHD3-10 IGHJ6 agtgggtccttcagtggttacctc tggacctggatccgccagtcccccgggaaggggctggagtggattggagaa attaattatagtgggagaacc aactacaacccgacccttgagagtcaagtcaccatttctgtagacacgtccaagaaccaattctccctgaagcttacctctgtgaccgccgcggacacggctgtctattactgt gcgagaggtcacagaaatccgggagtttgggacctcggtctggacgtc IGA1 21 210 11 10 8 13 3 8 8 2 6 5 JY8QFUQ01AY94E 6120 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*02 F or Homsap IGHV4-39*05 F 992 94.57 209/221 nt 94.12 208/221 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-6*01 F 3 10 7 12 10.7.12 [1.17.38.11] CARHSRLVRYFEYW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 69 of V-REGION: 'C' (1 nucleotides at position 118 to 118 in the user submitted sequence), (cause frameshift); tctctggtgactccatcagcagtagttcttactactggggctggatccgccagcccccagggaaggggctggagtggattgggagtatctttcatagagggagcacctactccaaccCcgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccttttctccctgaatctgagctctgtgaccgccacagacacggctgtttattactgtgcgagacattcgagactcgtccgttactttgaatactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 89 [289, 288] g83>a,G28>D(- - -)|a106>t|g107>c|a170>t,Y57>F(- + -)|t172>c,Y58>H(+ - -)|t177>a,S59>R(- - -)|a200>c,Y67>S(+ - -)|a257>t,Q86>L(- - -)|g258>t,Q86>L(- - -)|g270>t,K90>N(+ - -)|g289>a,A97>T(- - -)|g303>t| g83>a,G28>D(- - -)|a106>t|g107>c| a170>t,Y57>F(- + -)|t172>c,Y58>H(+ - -)|t177>a,S59>R(- - -)| a200>c,Y67>S(+ - -)|a257>t,Q86>L(- - -)|g258>t,Q86>L(- - -)|g270>t,K90>N(+ - -)|g289>a,A97>T(- - -)|g303>t| 320 229 217 12 3 9 0 2 0 1 1 0 3 1 1 0 3 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 2 1 0 1 0 0 0 0 1 0 1 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 18 3 0 3 0 0 0 1 0 0 1 1 0 0 0 0 117 114 108 6 1 5 0 1 0 0 1 0 1 0 0 0 3 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatcagcagtagttcttactac tggggctggatccgccagcccccagggaaggggctggagtggattgggagt atctttcatagagggagcacc tactccaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccttttctccctgaatctgagctctgtgaccgccacagacacggctgtttattactgt gcgagacattcgagactcgtccgttactttgaatac ARHSRLVRYFEY IGHV4-39 IGHD6-6 IGHJ4 ggtgactccatcagcagtagttcttactac tggggctggatccgccagcccccagggaaggggctggagtggattgggagt atctttcatagagggagcacc tactccaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccttttctccctgaatctgagctctgtgaccgccacagacacggctgtttattactgt gcgagacattcgagactcgtccgttactttgaatac IGA2 12 216 3 9 2 6 1 6 1 2 5 4 JY8QFUQ01AYCCC 15393 productive Homsap IGHV1-46*01 F, or Homsap IGHV1-46*02 F or Homsap IGHV1-46*03 F 894 91.12 195/214 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 165 80.39 41/51 nt Homsap IGHD5-18*01 F 1 8 8 15 8.8.15 [0.17.38.11] CAIVDTNLVSATGKDSW in-frame - Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) tggatacaccttcagcaactactatagacactgggtgcgacaggcccctggacaagggcttgagtggatgggaatgatcaaccctagtagtgattacaaatattacgcacagaaatttcagggcagagtcaccatgaccagggacacgtccacgagcacagtcttcatggagctgagtagcctgagatctgacgacacggccgtgtattattgtgcaatagtggacacaaatttggtttcggcgaccggtaaagactcctggggccagggaaccctggtcaccgtcgcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [290] c104>g,T35>S(+ - +)|g107>a,S36>N(- - -)|t116>g,M39>R(- + -)|g117>a,M39>R(- + -)|a165>g,I55>M(+ + -)|g184>a,G62>S(+ + -)|g188>a,G63>D(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|c194>a,T65>K(- - -)|a196>t,S66>Y(+ - -)|g197>a,S66>Y(+ - -)|c198>t,S66>Y(+ - -)|g210>a|c213>t|a263>t,Y88>F(- + -)|c276>t|g291>c,E97>D(+ - +)|c309>t|g315>a|g317>t,R106>I(- + -)| c104>g,T35>S(+ - +)|g107>a,S36>N(- - -)| t116>g,M39>R(- + -)|g117>a,M39>R(- + -)|a165>g,I55>M(+ + -)| g184>a,G62>S(+ + -)|g188>a,G63>D(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|c194>a,T65>K(- - -)| a196>t,S66>Y(+ - -)|g197>a,S66>Y(+ - -)|c198>t,S66>Y(+ - -)|g210>a|c213>t|a263>t,Y88>F(- + -)|c276>t|g291>c,E97>D(+ - +)|c309>t| g315>a|g317>t,R106>I(- + -)|a320>t| 319 (320) 221 (222) 200 21 (22) 5 (6) 16 1 8 4 0 0 1 3 (4) 0 1 1 1 1 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 0 0 1 0 0 51 51 48 3 0 3 1 1 0 0 0 0 0 0 0 0 0 1 30 24 19 5 0 5 0 3 0 0 0 1 1 0 0 0 0 0 117 114 105 9 4 5 0 2 4 0 0 0 2 0 1 0 0 0 7 (8) 7 5 2 (3) 1 (2) 1 0 1 0 0 0 0 0 (1) 0 0 0 1 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,162-163(FR2)|ta,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,62-63(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,164-165(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggca,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|aacc,169-172(CDR2)|agct,196-199(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcagcaactactat agacactgggtgcgacaggcccctggacaagggcttgagtggatgggaatg atcaaccctagtagtgattacaaa tattacgcacagaaatttcagggcagagtcaccatgaccagggacacgtccacgagcacagtcttcatggagctgagtagcctgagatctgacgacacggccgtgtattattgt gcaatagtggacacaaatttggtttcggcgaccggtaaagactcc AIVDTNLVSATGKDS IGHV1-46 IGHD5-18 IGHJ5 ggatacaccttcagcaactactat agacactgggtgcgacaggcccctggacaagggcttgagtggatgggaatg atcaaccctagtagtgattacaaa tattacgcacagaaatttcagggcagagtcaccatgaccagggacacgtccacgagcacagtcttcatggagctgagtagcctgagatctgacgacacggccgtgtattattgt gcaatagtggacacaaatttggtttcggcgaccggtaaagactcc IGA1 19 213 12 7 11 14 1 5 4 0 8 7 JY8QFUQ01AYFQ1 21484 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 891 90.32 196/217 nt 89.4 194/217 nt Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD3-10*01 F 1 8 8 14 8.8.14 [1.17.38.11] CVRQLGFGDLFRFDPW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 42 of V-REGION: 'G' (1 nucleotides at position 38 to 38 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 48 of V-REGION: 'AG' (2 nucleotides at position 59 to 60 in the user submitted sequence), (cause frameshift); ctctggatacagttttaacagttatgccatgacttggGgtccgccaggctccagggaaAGggggctggagtgggtctcaactattagtggcactggtggtaaccaatactacgcagactccgtgaggggccggctcaccatctccagagacaattccaagaacacactatttctgcagatgagcagcctgagagccgaggacacggccgtttattactgtgtgagacaattgggattcggggacttattccggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [293] t83>a,F28>Y(- + -)|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>a,S35>N(- - -)|c108>t|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g163>a,A55>T(- - -)|t174>c|g176>c,S59>T(+ - +)|g191>a,S64>N(- - -)|a193>c,T65>Q(- - -)|c194>a,T65>Q(- - -)|a215>g,K72>R(+ + +)|t226>c,F76>L(+ - -)|g258>a|g261>a|a263>t,Y88>F(- + -)|a270>g|a275>g,N92>S(- - -)|a303>t|c314>t,A105>V(+ - +)| t83>a,F28>Y(- + -)|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>a,S35>N(- - -)|c108>t| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g163>a,A55>T(- - -)| t174>c|g176>c,S59>T(+ - +)|g191>a,S64>N(- - -)|a193>c,T65>Q(- - -)|c194>a,T65>Q(- - -)| a215>g,K72>R(+ + +)|t226>c,F76>L(+ - -)|g258>a|g261>a|a263>t,Y88>F(- + -)|a270>g|a275>g,N92>S(- - -)|a303>t| c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)|g319>c| 316 (320) 221 (225) 199 (201) 22 (24) 6 (7) 16 (17) 3 (4) 5 4 2 1 1 2 1 2 (3) 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 2 0 0 0 0 1 0 1 0 0 51 51 48 3 0 3 0 1 1 0 0 0 0 0 1 0 0 0 30 24 19 5 1 4 0 1 0 1 1 1 0 0 1 0 0 0 117 114 106 8 4 4 3 2 0 1 0 0 2 0 0 0 0 0 4 (8) 4 3 (5) 1 (3) 0 (1) 1 (2) 0 (1) 0 1 0 0 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatacagttttaacagttatgcc atgacttgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtggcactggtggtaaccaa tactacgcagactccgtgaggggccggctcaccatctccagagacaattccaagaacacactatttctgcagatgagcagcctgagagccgaggacacggccgtttattactgt gtgagacaattgggattcggggacttattccggttcgacccc VRQLGFGDLFRFDP IGHV3-23 IGHD3-10 IGHJ5 ggatacagttttaacagttatgcc atgacttgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtggcactggtggtaaccaa tactacgcagactccgtgaggggccggctcaccatctccagagacaattccaagaacacactatttctgcagatgagcagcctgagagccgaggacacggccgtttattactgt gtgagacaattgggattcggggacttattccggttcgacccc IGA2 21 213 13 8 8 12 5 9 4 2 7 8 JY8QFUQ01AYG87 10925 productive (see comment) Homsap IGHV3-30*01 F 873 89.4 194/217 nt 88.94 193/217 nt Homsap IGHJ6*02 F (see comment) 184 77.42 48/62 nt Homsap IGHD3-10*01 F 3 8 8 18 8.8.18 [1.17.37.11] CARGTMLRGVTKARYGLDVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) in FR3-IMGT, from codon 101 of V-REGION: 1 nucleotides (from position 209 in the user submitted sequence), (cause frameshift) cctctggattcaccttcagtacctatgctatgtactgggtccgccaggctccaggcaaggggccagagtgggtgtcagtgatatcacatgatggaaataaggaagaatacgcagactccgtgaagggccgattcaccatttccagagacaactccaagaaaatgttgtacctgcaaatgaacaaccagcgacctgatgacacggctgttattattgtgcgagagggactatgcttcggggagtcaccaaagcgagatacggtctggacgtctggggccaagggaccacggtcatcgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [303] g107>c,S36>T(+ - +)|c118>t,H40>Y(+ - -)|t149>c,L50>P(- - -)|g160>t,A54>S(- + -)|t165>g|t172>c,Y58>H(+ - -)|g188>a,S63>N(- - -)|t192>g,N64>K(+ - -)|a193>g,K65>E(+ - -)|t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|c234>t|t246>c|c255>a,N85>K(+ - -)|c257>t,T86>M(- - -)|c259>t|t264>c|g278>a,S93>N(- - -)|t281>a,L94>Q(- - -)|a283>c|g286>c,A96>P(- - -)|g291>t,E97>D(+ - +)|c309>t| g107>c,S36>T(+ - +)| c118>t,H40>Y(+ - -)|t149>c,L50>P(- - -)|g160>t,A54>S(- + -)|t165>g| t172>c,Y58>H(+ - -)|g188>a,S63>N(- - -)|t192>g,N64>K(+ - -)|a193>g,K65>E(+ - -)| t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|c234>t|t246>c|c255>a,N85>K(+ - -)|c257>t,T86>M(- - -)|c259>t|t264>c|g278>a,S93>N(- - -)|t281>a,L94>Q(- - -)|a283>c|g286>c,A96>P(- - -)|g291>t,E97>D(+ - +)|c309>t| a320>g| 319 (320) 224 (225) 201 23 (24) 7 (8) 16 1 (2) 2 5 4 1 2 0 1 2 0 2 3 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 47 4 1 3 0 0 1 1 0 0 0 0 0 0 1 1 30 24 20 4 0 4 1 1 0 1 0 0 0 0 0 0 0 1 117 113 99 14 6 8 0 1 4 2 1 2 0 1 1 0 1 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtacctatgct atgtactgggtccgccaggctccaggcaaggggccagagtgggtgtcagtg atatcacatgatggaaataaggaa gaatacgcagactccgtgaagggccgattcaccatttccagagacaactccaagaaaatgttgtacctgcaaatgaacaaccagcgacctgatgacacggctgttattattgt gcgagagggactatgcttcggggagtcaccaaagcgagatacggtctggacgtc ARGTMLRGVTKARYGLDV IGHV3-30 IGHD3-10 IGHJ6 ggattcaccttcagtacctatgct atgtactgggtccgccaggctccaggcaaggggccagagtgggtgtcagtg atatcacatgatggaaataaggaa gaatacgcagactccgtgaagggccgattcaccatttccagagacaactccaagaaaatgttgtacctgcaaatgaacaaccagcgacctgatgacacggctgttattattgt gcgagagggactatgcttcggggagtcaccaaagcgagatacggtctggacgtc IGG1 23 212 12 11 7 13 5 10 7 0 11 5 JY8QFUQ01AYHQQ 3454 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 979 93.69 208/222 nt NA Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD2-15*01 F 2 8 10 16 8.10.16 [1.17.38.11] CAADGCPGGTCYHGFDMW in-frame + tctggattcactttcagtaacgtctggatgcactgggtccgccaggctccagggaaggggctggagtgggttggccgtattagaagcaaaactgctggtggggcaacagaatacgctgcgcccgtgagaggcatattctccatctcaagagatgattcaaagaacacgttgtatctgcaaatgaacagcctgaagaccgaggacacagccgtgtattactgtgccgcagatggttgtcctggtggtacctgctatcatggttttgatatgtggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 71 100 [301] c110>t,A37>V(+ - +)|a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)|a170>g,K57>R(+ + +)|a182>c,D61>A(- - -)|a190>g,T64>A(- - -)|c198>a,D66>E(+ - +)|a207>g|a215>g,K72>R(+ + +)|g224>t,R75>I(- + -)|a229>t,T77>S(+ - +)|a252>g|c259>t|a285>g|a313>g,T105>A(- - -)|a316>g,T106>A(- - -)| c110>t,A37>V(+ - +)| a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)| a170>g,K57>R(+ + +)|a182>c,D61>A(- - -)|a190>g,T64>A(- - -)| c198>a,D66>E(+ - +)|a207>g|a215>g,K72>R(+ + +)|g224>t,R75>I(- + -)|a229>t,T77>S(+ - +)|a252>g|c259>t|a285>g| a313>g,T105>A(- - -)|a316>g,T106>A(- - -)| 320 230 214 16 4 12 8 1 2 0 2 1 1 0 0 0 1 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 1 0 0 1 0 0 0 0 0 0 0 30 30 27 3 0 3 2 0 0 0 1 0 0 0 0 0 0 0 117 114 106 8 4 4 4 0 1 0 0 1 1 0 0 0 1 0 8 8 6 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| tct ggattcactttcagtaacgtctgg atgcactgggtccgccaggctccagggaaggggctggagtgggttggccgt attagaagcaaaactgctggtggggcaaca gaatacgctgcgcccgtgagaggcatattctccatctcaagagatgattcaaagaacacgttgtatctgcaaatgaacagcctgaagaccgaggacacagccgtgtattactgt gccgcagatggttgtcctggtggtacctgctatcatggttttgatatg AADGCPGGTCYHGFDM IGHV3-15 IGHD2-15 IGHJ3 ggattcactttcagtaacgtctgg atgcactgggtccgccaggctccagggaaggggctggagtgggttggccgt attagaagcaaaactgctggtggggcaaca gaatacgctgcgcccgtgagaggcatattctccatctcaagagatgattcaaagaacacgttgtatctgcaaatgaacagcctgaagaccgaggacacagccgtgtattactgt gccgcagatggttgtcctggtggtacctgctatcatggttttgatatg IGA1 14 219 9 5 3 5 6 9 4 0 6 4 JY8QFUQ01AYHQY 9718 productive (see comment) Homsap IGHV3-7*01 F 1004 95.87 209/218 nt 95.41 208/218 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 7 8 11 7.8.11 [2.17.38.11] CAISSGYYSTGYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 30 of V-REGION: 1 nucleotides (from position 18 in the user submitted sequence), (cause frameshift) ggctctggattcagcttagttactattggatgagctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaaggaagatggaagtgagagacactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgtgcgattagtagtggttattactcgacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [282] c74>g,A25>G(- + -)|c86>g,T29>S(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)|t196>c,Y66>H(+ - -)|a254>g,N85>S(- - -)|g303>a| c74>g,A25>G(- + -)| c86>g,T29>S(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)| c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)| t196>c,Y66>H(+ - -)|a254>g,N85>S(- - -)|g303>a| g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 316 (320) 222 (226) 213 9 (13) 1 (3) 8 (10) 2 (3) 2 (3) 0 1 0 0 1 (2) 0 0 3 0 (1) 0 78 6 5 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 36 23 20 3 0 3 0 1 0 0 0 0 1 0 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 1 0 0 117 114 111 3 1 2 1 1 0 1 0 0 0 0 0 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ggctct ggattcagcttagttactattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgattagtagtggttattactcgacgggctac AISSGYYSTGY IGHV3-7 IGHD3-22 IGHJ4 ggattcagcttagttactattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgattagtagtggttattactcgacgggctac IGA2 8 212 5 3 2 4 3 4 1 0 2 5 JY8QFUQ01AYINE 20760 productive Homsap IGHV3-9*01 F 932 92.2 201/218 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 2 8 8 16 8.8.16 [1.17.38.11] CAKDGSAIPVTGTYFEYW in-frame + cctctggattcacctttcatgattataccatgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagtattagttggaatagtggtaacatagactatgcggcctctgtgaggggccgattcaccgtctccagagacaacgccaataactccctgtctcttcagatgaatggtctgagatctgaggacacggccctctattactgtgcaaaagacgggtcggcgataccagtgactggtacctactttgaatactggggccagggagccctggtcaccgtctcctcagcttccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 93 100 [298] g103>c,D35>H(- - -)|g112>a,A38>T(- - -)|g163>a,G55>S(+ + -)|g191>a,S64>N(- - -)|g197>a,G66>D(- - -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|g252>t,K84>N(+ - -)|a263>c,Y88>S(+ - -)|g267>t|a270>g|c276>t|a277>g,S93>G(+ + -)|g286>t,A96>S(- + -)|t301>c|g303>c| g103>c,D35>H(- - -)|g112>a,A38>T(- - -)| g163>a,G55>S(+ + -)| g191>a,S64>N(- - -)| g197>a,G66>D(- - -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|g252>t,K84>N(+ - -)|a263>c,Y88>S(+ - -)|g267>t|a270>g|c276>t|a277>g,S93>G(+ + -)|g286>t,A96>S(- + -)|t301>c|g303>c| t321>c|a322>g| 320 (322) 226 (228) 209 17 (19) 5 (7) 12 4 (5) 4 1 1 (2) 2 0 0 0 2 0 3 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 117 114 101 13 5 8 4 1 1 1 2 0 0 0 1 0 3 0 8 (10) 8 8 0 (2) 0 (2) 0 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttcatgattatacc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt attagttggaatagtggtaacata gactatgcggcctctgtgaggggccgattcaccgtctccagagacaacgccaataactccctgtctcttcagatgaatggtctgagatctgaggacacggccctctattactgt gcaaaagacgggtcggcgataccagtgactggtacctactttgaatac AKDGSAIPVTGTYFEY IGHV3-9 IGHD6-19 IGHJ4 ggattcacctttcatgattatacc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt attagttggaatagtggtaacata gactatgcggcctctgtgaggggccgattcaccgtctccagagacaacgccaataactccctgtctcttcagatgaatggtctgagatctgaggacacggccctctattactgt gcaaaagacgggtcggcgataccagtgactggtacctactttgaatac IGG2 17 213 10 7 5 10 5 7 5 0 9 3 JY8QFUQ01AYL0X 14208 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 807 93.48 172/184 nt 92.93 171/184 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD6-13*01 F 3 8 8 11 8.8.11 [1.6.38.11] CVRDRSGQAVGYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 41 of V-REGION: 33 nucleotides (from position 35 in the user submitted sequence), (do not cause frameshift) ctctggattcaccttcagtagttactggatgcactgggtctcacgaattaatactgatgggagtgccacaagttacgcggactccgtgaggggccgattcaccatctccagagacaacgccaagaacacgctatatcttcaaatgaacagtctgagagtcgaagacacggctgtctattactgtgtaagagatcggtccggtcaggcggtaggatactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [249] c108>t|t165>a|g173>c,S58>T(+ - +)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|c198>t|a215>g,K72>R(+ + +)|g261>a|g267>t|c287>t,A96>V(+ - +)|g291>a|g303>c|c314>t,A105>V(+ - +)| c108>t| t165>a| g173>c,S58>T(+ - +)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| c198>t|a215>g,K72>R(+ + +)|g261>a|g267>t|c287>t,A96>V(+ - +)|g291>a|g303>c| c314>t,A105>V(+ - +)| 320 192 179 13 7 6 2 2 4 0 0 0 0 1 3 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 18 17 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 2 0 0 0 117 114 107 7 5 2 1 2 2 0 0 0 0 0 1 0 1 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagttactgg atgcactgggtctcacga attaatactgatgggagtgccaca agttacgcggactccgtgaggggccgattcaccatctccagagacaacgccaagaacacgctatatcttcaaatgaacagtctgagagtcgaagacacggctgtctattactgt gtaagagatcggtccggtcaggcggtaggatac VRDRSGQAVGY IGHV3-74 IGHD6-13 IGHJ4 ggattcaccttcagtagttactgg atgcactgggtctcacga attaatactgatgggagtgccaca agttacgcggactccgtgaggggccgattcaccatctccagagacaacgccaagaacacgctatatcttcaaatgaacagtctgagagtcgaagacacggctgtctattactgt gtaagagatcggtccggtcaggcggtaggatac IGG2 12 180 7 5 5 9 2 3 6 1 2 3 JY8QFUQ01AYR4C 10954 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 981 94.93 206/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD1-26*01 F 1 8 8 12 8.8.12 [1.17.38.11] CARDILGATRFDYW in-frame + ctctggattcaccttcagcagctatgccatgagctgggtccgccaggctccagggaagggcctggagtgggtctcagctattagtggtattggtgggagtacattctacgcagactccgtgaagggccggttcaccatcaccagagacaattccaacaacacgctgtttctgcaaatgaatagcctgagagccgaggacacggccatatattactgtgcgagagatattctgggagctactcggtttgactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [284] t90>c|g147>c|g176>t,S59>I(- - -)|t189>g|c192>t|a197>t,Y66>F(- + -)|t235>a,S79>T(+ - +)|g252>c,K84>N(+ - -)|a263>t,Y88>F(- + -)|c276>t|g301>a,V101>I(+ - +)|a317>g,K106>R(+ + +)| t90>c| g147>c| g176>t,S59>I(- - -)|t189>g|c192>t| a197>t,Y66>F(- + -)|t235>a,S79>T(+ - +)|g252>c,K84>N(+ - -)|a263>t,Y88>F(- + -)|c276>t|g301>a,V101>I(+ - +)| a317>g,K106>R(+ + +)| 320 225 213 12 5 7 1 1 2 1 0 0 2 1 2 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 21 3 2 1 0 0 1 0 0 0 0 0 0 0 1 1 117 114 108 6 1 5 0 1 1 0 0 0 2 1 1 0 0 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagcagctatgcc atgagctgggtccgccaggctccagggaagggcctggagtgggtctcagct attagtggtattggtgggagtaca ttctacgcagactccgtgaagggccggttcaccatcaccagagacaattccaacaacacgctgtttctgcaaatgaatagcctgagagccgaggacacggccatatattactgt gcgagagatattctgggagctactcggtttgactat ARDILGATRFDY IGHV3-23 IGHD1-26 IGHJ4 ggattcaccttcagcagctatgcc atgagctgggtccgccaggctccagggaagggcctggagtgggtctcagct attagtggtattggtgggagtaca ttctacgcagactccgtgaagggccggttcaccatcaccagagacaattccaacaacacgctgtttctgcaaatgaatagcctgagagccgaggacacggccatatattactgt gcgagagatattctgggagctactcggtttgactat IGA2 11 213 4 7 3 6 1 5 2 3 5 1 JY8QFUQ01AYTLP 11748 productive (see comment) Homsap IGHV3-7*01 F (see comment) 742 82.87 179/216 nt 81.94 177/216 nt Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD2-15*01 F 1 8 7 15 8.7.15 [1.17.38.11] CARLKGLTHHDFYFDLW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (82,87% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR3-IMGT at codon 72 of V-REGION: 'G' (1 nucleotides at position 122 to 122 in the user submitted sequence), (cause frameshift); in CDR2-IMGT, from codon 63 of V-REGION: 1 nucleotides (from position 96 in the user submitted sequence), (cause frameshift) ctctggattcgtctttactaatcattggatgagttgggtccgccaggccacagggaaggggccggagtgggtggccaacatatccccagacggaatacgaaatattttggggactctgtgaGggggccgattcagcgtctccagagacaacggcaagcagtcatcgtatctggaaatgaataccctgacagtcgatgacacggctgtatacttctgtgcgaggttgaaggggctgacacatcacgacttctacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [294] a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>c,Y37>H(+ - -)|c120>t|t135>c|c136>a,P46>T(+ + -)|t149>c,L50>P(- - -)|a169>t,K57>S(- - -)|a170>c,K57>S(- - -)|g171>c,K57>S(- - -)|a173>c,Q58>P(- - -)|t177>c|g190>a,E64>T(- - -)|a191>c,E64>T(- - -)|c198>t|a200>t,Y67>F(- + -)|t203>g,V68>G(- - -)|a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|a232>g,I78>V(+ - +)|c248>g,A83>G(- + -)|a253>c,N85>Q(+ - +)|c255>g,N85>Q(+ - +)|c259>t,L87>S(- - -)|t260>c,L87>S(- - -)|c268>g,Q90>E(+ + -)|c276>t|g278>c,S93>T(+ - +)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)|g303>a|t306>c|a308>t,Y103>F(- + -)| a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>c,Y37>H(+ - -)| c120>t|t135>c|c136>a,P46>T(+ + -)|t149>c,L50>P(- - -)| a169>t,K57>S(- - -)|a170>c,K57>S(- - -)|g171>c,K57>S(- - -)|a173>c,Q58>P(- - -)|t177>c|g190>a,E64>T(- - -)|a191>c,E64>T(- - -)| c198>t|a200>t,Y67>F(- + -)|t203>g,V68>G(- - -)|a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|a232>g,I78>V(+ - +)|c248>g,A83>G(- + -)|a253>c,N85>Q(+ - +)|c255>g,N85>Q(+ - +)|c259>t,L87>S(- - -)|t260>c,L87>S(- - -)|c268>g,Q90>E(+ + -)|c276>t|g278>c,S93>T(+ - +)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)|g303>a|t306>c|a308>t,Y103>F(- + -)| a318>g|g319>t|a320>t| 317 (320) 221 (224) 184 37 (40) 7 (10) 30 3 (4) 3 7 6 4 1 3 (4) 0 4 4 1 (2) 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 1 2 1 0 0 0 0 1 0 0 0 51 51 47 4 2 2 0 0 1 2 0 1 0 0 0 0 0 0 30 23 16 7 1 6 0 1 0 1 3 0 1 0 1 0 0 0 117 114 94 20 4 16 2 1 4 2 1 0 2 0 2 4 1 1 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcgtctttactaatcattgg atgagttgggtccgccaggccacagggaaggggccggagtgggtggccaac atatccccagacggaatacgaaa tattttggggactctgtgaggggccgattcagcgtctccagagacaacggcaagcagtcatcgtatctggaaatgaataccctgacagtcgatgacacggctgtatacttctgt gcgaggttgaaggggctgacacatcacgacttctacttcgatctc ARLKGLTHHDFYFDL IGHV3-7 IGHD2-15 IGHJ2 ggattcgtctttactaatcattgg atgagttgggtccgccaggccacagggaaggggccggagtgggtggccaac atatccccagacggaatacgaaa tattttggggactctgtgaggggccgattcagcgtctccagagacaacggcaagcagtcatcgtatctggaaatgaataccctgacagtcgatgacacggctgtatacttctgt gcgaggttgaaggggctgacacatcacgacttctacttcgatctc IGG1 37 212 19 18 10 20 9 17 6 1 18 12 JY8QFUQ01AYXP4 13868 productive (see comment) Homsap IGHV4-61*02 F 974 93.67 207/221 nt 92.76 205/221 nt Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD3-10*01 F 3 10 7 11 10.7.11 [1.17.38.11] CATVARRGAFDIW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 78 of V-REGION: 'C' (1 nucleotides at position 141 to 141 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 95 of V-REGION: 'G' (1 nucleotides at position 194 to 194 in the user submitted sequence), (cause frameshift); tctctggtgggtccatcagcagtactagttactactggagctggatccggcagcccgccgggaagggactggagtggatggggcgtatctataccagtgggatcaccaactacaacccctccctcgagagtcgagtcaccCttttcagtggacacgtccaagaaccagttctccctgaagctgaagtctgtaaGcccccgcagacacggccgtttattactgtgcgacagtggcgcgccggggggcttttgatatctggggccaagggacactggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [288] c84>g|g103>a,G35>T(+ - -)|g104>c,G35>T(+ - -)|t159>g,I53>M(+ + -)|g191>t,S64>I(- - -)|a214>g,K72>E(+ - -)|a232>t,I78>F(+ - -)|a234>t,I78>F(+ - -)|a240>g|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g282>a|g286>c,A96>P(- - -)|g303>t|g317>c,R106>T(- - -)| c84>g|g103>a,G35>T(+ - -)|g104>c,G35>T(+ - -)| t159>g,I53>M(+ + -)| g191>t,S64>I(- - -)| a214>g,K72>E(+ - -)|a232>t,I78>F(+ - -)|a234>t,I78>F(+ - -)|a240>g|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g282>a|g286>c,A96>P(- - -)|g303>t| g317>c,R106>T(- - -)|a320>t| 319 (320) 228 (229) 213 15 (16) 4 (5) 11 2 3 0 0 0 0 2 (3) 0 3 2 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 1 2 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 30 21 20 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 117 114 105 9 3 6 2 2 0 0 0 0 2 0 1 1 1 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 0 0 0 0 0 (1) 0 1 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgggtccatcagcagtactagttactac tggagctggatccggcagcccgccgggaagggactggagtggatggggcgt atctataccagtgggatcacc aactacaacccctccctcgagagtcgagtcaccttttcagtggacacgtccaagaaccagttctccctgaagctgaagtctgtaacccccgcagacacggccgtttattactgt gcgacagtggcgcgccggggggcttttgatatc ATVARRGAFDI IGHV4-61 IGHD3-10 IGHJ3 ggtgggtccatcagcagtactagttactac tggagctggatccggcagcccgccgggaagggactggagtggatggggcgt atctataccagtgggatcacc aactacaacccctccctcgagagtcgagtcaccttttcagtggacacgtccaagaaccagttctccctgaagctgaagtctgtaacccccgcagacacggccgtttattactgt gcgacagtggcgcgccggggggcttttgatatc IGG2 14 216 5 9 3 9 2 5 3 1 7 3 JY8QFUQ01AYY7V 9584 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 827 87.44 188/215 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD4-23*01 ORF 3 8 7 12 8.7.12 [1.17.38.11] CASSVFSPGGFDYW in-frame + cctctggactcagtttcactaacgcctggatgagctgggtccgccaggctccggggaagggactggagtctgtctctgttttttatagcggtgatcaaacatactacgcagactccgtgaggggccgattcaccatctccatagacaattccaagaacacactgtatcttcaaatgaacggcctgcgagccgaggacacggccgtgtattattgtgcgagctccgtgtttagtcccggaggctttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccctaaagatgggaacgtggtcgtcgcatgcctggtccagggcttctt NA IGA2 71 95 [282, 285] g81>a|t82>c,F28>L(+ - -)|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g88>t,V30>F(+ - -)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|a109>g,N37>A(- - -)|a110>c,N37>A(- - -)|a113>g,Y38>W(- + -)|c114>g,Y38>W(- + -)|a138>g|g147>a|g155>c,W52>S(- - -)|g156>t,W52>S(- - -)|a162>t|a166>t,I56>F(+ - -)|g188>a,G63>D(- - -)|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>a,S64>Q(- - -)|a215>g,K72>R(+ + +)|g239>t,R80>I(- + -)|g258>a|a277>g,S93>G(+ + -)|a283>c|c309>t| g81>a|t82>c,F28>L(+ - -)|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g88>t,V30>F(+ - -)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|a109>g,N37>A(- - -)|a110>c,N37>A(- - -)|a113>g,Y38>W(- + -)|c114>g,Y38>W(- + -)| a138>g|g147>a|g155>c,W52>S(- - -)|g156>t,W52>S(- - -)|a162>t| a166>t,I56>F(+ - -)|g188>a,G63>D(- - -)|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>a,S64>Q(- - -)| a215>g,K72>R(+ + +)|g239>t,R80>I(- + -)|g258>a|a277>g,S93>G(+ + -)|a283>c|c309>t| a318>c,R106>S(- - -)|g319>t|a320>c| 317 (320) 220 (223) 193 27 (30) 7 (9) 20 (21) 5 6 2 1 3 (5) 1 2 0 2 2 3 (4) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 13 11 1 10 2 2 1 1 1 0 0 0 1 2 1 0 51 51 46 5 3 2 1 1 0 0 0 0 1 0 1 0 1 0 30 21 16 5 0 5 0 2 0 0 1 1 1 0 0 0 0 0 117 114 108 6 3 3 2 1 1 0 1 0 0 0 0 0 1 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 0 0 0 0 (2) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggactcagtttcactaacgcctgg atgagctgggtccgccaggctccggggaagggactggagtctgtctctgtt ttttatagcggtgatcaaaca tactacgcagactccgtgaggggccgattcaccatctccatagacaattccaagaacacactgtatcttcaaatgaacggcctgcgagccgaggacacggccgtgtattattgt gcgagctccgtgtttagtcccggaggctttgactac ASSVFSPGGFDY IGHV3-53 IGHD4-23 IGHJ4 ggactcagtttcactaacgcctgg atgagctgggtccgccaggctccggggaagggactggagtctgtctctgtt ttttatagcggtgatcaaaca tactacgcagactccgtgaggggccgattcaccatctccatagacaattccaagaacacactgtatcttcaaatgaacggcctgcgagccgaggacacggccgtgtattattgt gcgagctccgtgtttagtcccggaggctttgactac IGA2 27 210 14 13 8 16 6 11 6 1 5 15 JY8QFUQ01AZ2OV 4273 productive Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 860 88.53 193/218 nt NA Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD3-10*01 F 1 8 8 11 8.8.11 [1.17.38.11] CTRVGTFNWFGPW in-frame + cctctggattcaactttgcagattatggcttgggctgggtccgccaacttccagggaaggggctggaatgggtcggtggtgttaattggaatgggggcagcgcaggttatgcagtctctgtggagggccgattcatcatctccagagacaatggcaagaagtccctatatttgcaaatgaacagtctgagagtcgaggacacggccgtgtattactgtacgagagtcggcacgttcaactggttcggcccctggggccagggaacgctggtcaccgtctcctctgcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [282] c86>a,T29>N(- + -)|a104>c,D35>A(- - -)|t105>a,D35>A(- - -)|a115>t,M39>L(+ + -)|a118>g,S40>G(+ + -)|g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|g153>a|t160>g,S54>G(+ + -)|c161>g,S54>G(+ + -)|a166>g,I56>V(+ - +)|t186>g|t189>c|a193>g,T65>A(- - -)|a206>t,D69>V(- - -)|a214>g,K72>E(+ - -)|c230>t,T77>I(- - -)|c246>t|c248>g,A83>G(- + -)|c255>g,N85>K(+ - -)|g261>a|c265>t|c287>t,A96>V(+ - +)|t301>g,L101>V(+ - +)|c307>t,H103>Y(+ - -)|g313>a,A105>T(- - -)| c86>a,T29>N(- + -)|a104>c,D35>A(- - -)|t105>a,D35>A(- - -)| a115>t,M39>L(+ + -)|a118>g,S40>G(+ + -)|g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|g153>a|t160>g,S54>G(+ + -)|c161>g,S54>G(+ + -)| a166>g,I56>V(+ - +)|t186>g|t189>c|a193>g,T65>A(- - -)| a206>t,D69>V(- - -)|a214>g,K72>E(+ - -)|c230>t,T77>I(- - -)|c246>t|c248>g,A83>G(- + -)|c255>g,N85>K(+ - -)|g261>a|c265>t|c287>t,A96>V(+ - +)|t301>g,L101>V(+ - +)|c307>t,H103>Y(+ - -)| g313>a,A105>T(- - -)|a320>t| 319 (320) 225 (226) 199 26 (27) 6 (7) 20 4 3 6 1 1 1 2 (3) 1 1 3 0 3 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 0 1 1 0 1 0 0 0 0 51 51 44 7 1 6 1 1 1 0 0 0 1 0 1 1 0 1 30 24 20 4 2 2 2 0 0 1 0 0 0 0 0 0 0 1 117 114 103 11 3 8 1 1 5 0 0 0 1 0 0 2 0 1 7 (8) 7 6 1 (2) 0 (1) 1 0 1 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| cctct ggattcaactttgcagattatggc ttgggctgggtccgccaacttccagggaaggggctggaatgggtcggtggt gttaattggaatgggggcagcgca ggttatgcagtctctgtggagggccgattcatcatctccagagacaatggcaagaagtccctatatttgcaaatgaacagtctgagagtcgaggacacggccgtgtattactgt acgagagtcggcacgttcaactggttcggcccc TRVGTFNWFGP IGHV3-20 IGHD3-10 IGHJ5 ggattcaactttgcagattatggc ttgggctgggtccgccaacttccagggaaggggctggaatgggtcggtggt gttaattggaatgggggcagcgca ggttatgcagtctctgtggagggccgattcatcatctccagagacaatggcaagaagtccctatatttgcaaatgaacagtctgagagtcgaggacacggccgtgtattactgt acgagagtcggcacgttcaactggttcggcccc IGA1 25 213 13 12 8 13 5 12 4 2 14 5 JY8QFUQ01AZ3Q8 16107 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 813 86.92 186/214 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-16*02 F 2 8 7 12 8.7.12 [1.17.38.11] CARAPGWMNPFDFW in-frame - ctccggtggctccatcaccacttactacatcagctggctccggcagcccccagggaagggactggagtggattgggtgtatctcttatggtggggacactacctacaactcctccctcaagagtcgagtcaccatatcaggacaagggtccacgcgccagttctccctgaggctgagctccgtgaccgttgcggacacggccgtgtattactgtgcgcgcgccccagggtggatgaatccctttgacttctggggccagggcactctggtcaccgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [281] t78>c|g104>c,S35>T(+ - +)|t105>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|t115>a,W39>I(+ - -)|g116>t,W39>I(+ - -)|g117>c,W39>I(+ - -)|a124>c,I42>L(+ + +)|a164>g,Y55>C(- - -)|a170>c,Y57>S(+ - -)|c174>t|a175>g,S59>G(+ + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c195>t|a197>c,N66>T(- + -)|c205>t,P69>S(+ - -)|t239>g,V80>G(- - -)|g241>c,D81>Q(+ - -)|c243>a,D81>Q(+ - -)|a244>g,T82>G(+ - -)|c245>g,T82>G(+ - -)|a251>c,K84>T(- - -)|a253>c,N85>R(+ - -)|a254>g,N85>R(+ - -)|a269>g,K90>R(+ + +)|t279>c|c287>t,A96>V(+ - +)| t78>c| g104>c,S35>T(+ - +)|t105>c,S35>T(+ - +)|g107>c,S36>T(+ - +)| t115>a,W39>I(+ - -)|g116>t,W39>I(+ - -)|g117>c,W39>I(+ - -)|a124>c,I42>L(+ + +)|a164>g,Y55>C(- - -)| a170>c,Y57>S(+ - -)|c174>t|a175>g,S59>G(+ + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c195>t| a197>c,N66>T(- + -)|c205>t,P69>S(+ - -)|t239>g,V80>G(- - -)|g241>c,D81>Q(+ - -)|c243>a,D81>Q(+ - -)|a244>g,T82>G(+ - -)|c245>g,T82>G(+ - -)|a251>c,K84>T(- - -)|a253>c,N85>R(+ - -)|a254>g,N85>R(+ - -)|a269>g,K90>R(+ + +)|t279>c|c287>t,A96>V(+ - +)| a316>c|a318>c|a320>c| 315 (320) 217 (222) 189 (191) 28 (31) 4 (7) 24 6 1 4 3 5 (8) 1 0 1 4 1 1 1 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 1 0 0 0 0 2 0 0 0 51 51 46 5 0 5 1 0 0 0 1 0 0 1 1 0 1 0 30 21 15 6 2 4 2 1 2 0 1 0 0 0 0 0 0 0 117 114 101 13 1 12 3 0 2 1 3 1 0 0 1 1 0 1 3 (8) 3 3 (5) 0 (3) 0 (3) 0 0 0 0 0 0 (3) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctcc ggtggctccatcaccacttactac atcagctggctccggcagcccccagggaagggactggagtggattgggtgt atctcttatggtggggacact acctacaactcctccctcaagagtcgagtcaccatatcaggacaagggtccacgcgccagttctccctgaggctgagctccgtgaccgttgcggacacggccgtgtattactgt gcgcgcgccccagggtggatgaatccctttgacttc ARAPGWMNPFDF IGHV4-59 IGHD3-16 IGHJ4 ggtggctccatcaccacttactac atcagctggctccggcagcccccagggaagggactggagtggattgggtgt atctcttatggtggggacact acctacaactcctccctcaagagtcgagtcaccatatcaggacaagggtccacgcgccagttctccctgaggctgagctccgtgaccgttgcggacacggccgtgtattactgt gcgcgcgccccagggtggatgaatccctttgacttc IGA2 27 210 13 14 5 12 8 15 1 2 17 7 JY8QFUQ01AZ72P 4338 productive (see comment) Homsap IGHV1-46*01 F, or Homsap IGHV1-46*03 F 934 93.43 199/213 nt 92.96 198/213 nt Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 219 92.16 47/51 nt Homsap IGHD6-13*01 F 2 8 8 21 8.8.21 [0.16.38.11] CARGWLTAADTGVASGHDWFDSW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR2-IMGT, from codon 49 of V-REGION: 1 nucleotides (from position 58 in the user submitted sequence), (cause frameshift) tggatacaccttcaccagctactatatgcactggatacgacaggcccctggacaaggcttgagtggatgggaataatcaaccctagtggtggtaccacaaggtacgcacagaagttccagggcagagtcttcatgaccagagacacgtccacgagcacagtccacatggaggtgaacggcgtaagatctgacgacacggccgtgtattactgtgcccgagggtggctgacagcagctgataccggggtggcgtcagggcacgattggttcgactcctggggccagggaaccctggtcaccgtctcctctgcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [307] g124>a,V42>I(+ - +)|g126>a,V42>I(+ - +)|g191>c,S64>T(+ - +)|c198>g,S66>R(- - -)|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|g240>a|t262>c,Y88>H(+ - -)|c271>g,L91>V(+ - +)|g275>a,S92>N(- - -)|a277>g,S93>G(+ + -)|c280>g,L94>V(+ - +)|g282>a,L94>V(+ - +)|g291>c,E97>D(+ - +)|g315>c|a316>c| g124>a,V42>I(+ - +)|g126>a,V42>I(+ - +)| g191>c,S64>T(+ - +)| c198>g,S66>R(- - -)|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|g240>a|t262>c,Y88>H(+ - -)|c271>g,L91>V(+ - +)|g275>a,S92>N(- - -)|a277>g,S93>G(+ + -)|c280>g,L94>V(+ - +)|g282>a,L94>V(+ - +)|g291>c,E97>D(+ - +)| g315>c|a316>c|a320>g| 319 (320) 220 (221) 204 16 (17) 3 (4) 13 1 (2) 5 1 1 1 0 1 0 3 3 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 50 48 2 0 2 0 2 0 0 0 0 0 0 0 0 0 0 30 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 117 114 103 11 1 10 1 3 1 1 0 0 1 0 1 3 0 0 7 (8) 7 5 2 (3) 2 (3) 0 0 (1) 0 0 0 1 0 0 0 1 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,162-163(FR2)|ta,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,62-63(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,164-165(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggca,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|aacc,169-172(CDR2)|agct,196-199(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcaccagctactat atgcactggatacgacaggcccctggacaaggcttgagtggatgggaata atcaaccctagtggtggtaccaca aggtacgcacagaagttccagggcagagtcttcatgaccagagacacgtccacgagcacagtccacatggaggtgaacggcgtaagatctgacgacacggccgtgtattactgt gcccgagggtggctgacagcagctgataccggggtggcgtcagggcacgattggttcgactcc ARGWLTAADTGVASGHDWFDS IGHV1-46 IGHD6-13 IGHJ5 ggatacaccttcaccagctactat atgcactggatacgacaggcccctggacaaggcttgagtggatgggaata atcaaccctagtggtggtaccaca aggtacgcacagaagttccagggcagagtcttcatgaccagagacacgtccacgagcacagtccacatggaggtgaacggcgtaagatctgacgacacggccgtgtattactgt gcccgagggtggctgacagcagctgataccggggtggcgtcagggcacgattggttcgactcc IGA1 14 212 8 6 6 11 2 3 1 0 12 1 JY8QFUQ01AZ89E 10194 productive Homsap IGHV4-61*02 F 1028 96.38 213/221 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-6*01 F 1 10 7 15 10.7.15 [1.17.38.11] CARAPSYTNSHQHCDYW in-frame + tctctggtggctccatcaacagtggtagttactactggagttggattcggcagcccgccgggaagggactggagtggattgggcgtatctataccagtgggagcaccaactacaacccctccctcaagagtcgagtcaccatatcaatggacacgtccaagaaccagttctccctgaagctgaactctgtgaccgccgcagacacggccgtctattattgtgcgagagctccctcttataccaactcgcaccagcattgtgactactggggccagggaaccctggtcaccgtctcctcagcatcccgacagccccaaggtcttcccgctgagcctcganagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccc NA IGA2 86 74 [295] g92>a,S31>N(- - -)|c120>t|c126>t|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|g275>a,S92>N(- - -)|g303>c|c309>t| g92>a,S31>N(- - -)| c120>t|c126>t| g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|g275>a,S92>N(- - -)|g303>c|c309>t| a320>c| 319 (320) 228 (229) 220 8 (9) 4 (5) 4 1 3 3 0 0 (1) 0 0 0 1 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 29 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 49 2 2 0 0 0 2 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 109 5 2 3 1 2 1 0 0 0 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcaacagtggtagttactac tggagttggattcggcagcccgccgggaagggactggagtggattgggcgt atctataccagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcaatggacacgtccaagaaccagttctccctgaagctgaactctgtgaccgccgcagacacggccgtctattattgt gcgagagctccctcttataccaactcgcaccagcattgtgactac ARAPSYTNSHQHCDY IGHV4-61 IGHD6-6 IGHJ4 ggtggctccatcaacagtggtagttactac tggagttggattcggcagcccgccgggaagggactggagtggattgggcgt atctataccagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcaatggacacgtccaagaaccagttctccctgaagctgaactctgtgaccgccgcagacacggccgtctattattgt gcgagagctccctcttataccaactcgcaccagcattgtgactac IGA2 8 216 7 1 6 7 1 1 4 0 3 1 JY8QFUQ01AZAYV 8477 productive Homsap IGHV3-11*01 F (see comment) 789 84.55 186/220 nt NA Homsap IGHJ6*02 F 247 88.71 55/62 nt Homsap IGHD1-26*01 F 3 8 8 15 8.8.15 [2.17.38.11] CARYRGSYYDYGGVDVW in-frame - Low V-REGION identity (84,55% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release aacctctggcttcagattccgtgactactacatgacgtgggtccgccaggctccagggaagggtcttgagtggctttcctccatcagcagcggtagtaataccatccactactcagactcggtgaggggccgcttcaccatctccagggacaacaccaggaactcagtggatctgcaaatgaatagtctgagagccgaagacacggccgtctattattgtgcgaggtacagaggcagttactacgactatggcggtgtggacgtctggggccaagggaccacggtcaccgtctccccagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [296, 295] g73>a,A25>T(- - -)|a81>c|c86>g,T29>R(- - -)|c87>a,T29>R(- - -)|a103>c,S35>R(- - -)|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|a124>g,I42>V(+ - +)|g147>t|g150>t|g157>c,V53>L(+ - +)|a162>c|a164>c,Y55>S(+ - -)|t168>c|t171>c|t174>c|a175>g,S59>G(+ + -)|g184>a,G62>S(+ + -)|g188>a,S63>N(- - -)|a195>c|t196>c,Y66>H(+ - -)|g202>t,A68>S(- + -)|t210>g|a215>g,K72>R(+ + +)|a225>c|g247>a,A83>T(- - -)|a251>g,K84>R(+ + +)|c259>g,L87>V(+ - +)|t262>g,Y88>D(- - -)|c276>t|c279>t|g291>a|g303>c|c309>t| g73>a,A25>T(- - -)| a81>c|c86>g,T29>R(- - -)|c87>a,T29>R(- - -)|a103>c,S35>R(- - -)| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|a124>g,I42>V(+ - +)|g147>t|g150>t|g157>c,V53>L(+ - +)|a162>c|a164>c,Y55>S(+ - -)| t168>c|t171>c|t174>c|a175>g,S59>G(+ + -)|g184>a,G62>S(+ + -)|g188>a,S63>N(- - -)|a195>c| t196>c,Y66>H(+ - -)|g202>t,A68>S(- + -)|t210>g|a215>g,K72>R(+ + +)|a225>c|g247>a,A83>T(- - -)|a251>g,K84>R(+ + +)|c259>g,L87>V(+ - +)|t262>g,Y88>D(- - -)|c276>t|c279>t|g291>a|g303>c|c309>t| a318>g|g319>t| 317 (320) 225 (228) 191 (192) 34 (36) 15 (17) 19 4 (5) 5 3 4 6 1 0 0 3 3 3 (4) 2 78 7 6 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 0 0 2 1 0 0 0 1 0 0 51 51 43 8 3 5 1 0 0 0 2 0 0 0 2 1 2 0 30 24 17 7 4 3 1 2 0 3 1 0 0 0 0 0 0 0 117 114 100 14 7 7 2 2 3 1 1 0 0 0 1 1 1 2 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| aacctct ggcttcagattccgtgactactac atgacgtgggtccgccaggctccagggaagggtcttgagtggctttcctcc atcagcagcggtagtaataccatc cactactcagactcggtgaggggccgcttcaccatctccagggacaacaccaggaactcagtggatctgcaaatgaatagtctgagagccgaagacacggccgtctattattgt gcgaggtacagaggcagttactacgactatggcggtgtggacgtc ARYRGSYYDYGGVDV IGHV3-11 IGHD1-26 IGHJ6 ggcttcagattccgtgactactac atgacgtgggtccgccaggctccagggaagggtcttgagtggctttcctcc atcagcagcggtagtaataccatc cactactcagactcggtgaggggccgcttcaccatctccagggacaacaccaggaactcagtggatctgcaaatgaatagtctgagagccgaagacacggccgtctattattgt gcgaggtacagaggcagttactacgactatggcggtgtggacgtc IGA2 33 213 15 18 7 17 8 16 10 5 12 6 JY8QFUQ01AZCLU 21803 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 1004 95.87 209/218 nt 95.41 208/218 nt Homsap IGHJ1*01 F (see comment) 134 73.08 38/52 nt Homsap IGHD3-10*01 F 3 8 8 14 8.8.14 [1.17.38.11] CAKDGARGAAVALTPG in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 and Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 61 to 61 in the user submitted sequence), (cause frameshift); cctctggattcacctttagaagctatgccatgagctgggtccgccaggttccagggaaggGggctggagtgggtctcagctattagtggtaatggtgctaacacatactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgtgcgaaagatggcgctagaggtgcggcagttgctttaactcctgggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [294, 293] c105>a,S35>R(- - -)|c134>t,A45>V(+ - +)|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|g191>a,S64>N(- - -)|g225>a|c248>t,S83>F(- - -)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)| c105>a,S35>R(- - -)| c134>t,A45>V(+ - +)| g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|g191>a,S64>N(- - -)| g225>a|c248>t,S83>F(- - -)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)| 320 226 217 9 1 8 0 3 2 0 1 1 0 0 2 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 2 0 0 0 0 0 0 1 0 0 0 117 114 110 4 1 3 0 1 1 0 1 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagaagctatgcc atgagctgggtccgccaggttccagggaaggggctggagtgggtctcagct attagtggtaatggtgctaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtgcggcagttgctttaactcct AKDGARGAAVALTP IGHV3-23 IGHD3-10 IGHJ1 ggattcacctttagaagctatgcc atgagctgggtccgccaggttccagggaaggggctggagtgggtctcagct attagtggtaatggtgctaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtgcggcagttgctttaactcct IGG2 9 213 5 4 5 8 0 1 1 0 4 4 JY8QFUQ01AZEES 17157 productive Homsap IGHV3-72*01 F 957 92.38 206/223 nt NA Homsap IGHJ6*02 F 193 79.03 49/62 nt Homsap IGHD6-19*01 F 1 8 10 14 8.10.14 [1.17.38.11] CARLHYSGGWYGLDVW in-frame + ctctggattcaccttcagtgaccactacatagactgggtccgccaggctccaggaaaggggctggagtgggttggccgtactcgaaataaagctaacggttacagtacagagtatgccgcgtctgtgaaaggcagattcaccgtctcaagagatgactcagagaacttagtgcatctgcaaatgaacagcctgaaaagcgaggacacggccctgtattactgtgctagactgcactacagtggtggctggtacggtctggacgtctggggccaagggaccacagtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [296] g117>a,M39>I(+ + -)|g141>a|a169>c|c174>t|a184>g,S62>G(+ + -)|c191>g,T64>S(+ - +)|c192>t,T64>S(+ - +)|a198>g|c201>t|a232>g,I78>V(+ - +)|t246>c|a250>g,K84>E(+ - -)|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)|c287>g,T96>S(+ - +)|g301>c,V101>L(+ - +)| g117>a,M39>I(+ + -)|g141>a| a169>c|c174>t|a184>g,S62>G(+ + -)|c191>g,T64>S(+ - +)|c192>t,T64>S(+ - +)| a198>g|c201>t|a232>g,I78>V(+ - +)|t246>c|a250>g,K84>E(+ - -)|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)|c287>g,T96>S(+ - +)|g301>c,V101>L(+ - +)| g319>c|a320>t| 318 (320) 229 (231) 212 17 (19) 6 (8) 11 4 2 4 2 1 0 0 (1) 0 1 (2) 3 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 30 30 25 5 2 3 1 0 2 0 1 0 0 0 0 1 0 0 117 114 104 10 3 7 3 0 2 2 0 0 0 0 1 2 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,165-168(FR2-CDR2)|agct,177-180(CDR2)|aact,253-256(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tgct,312-315(FR3)| ctct ggattcaccttcagtgaccactac atagactgggtccgccaggctccaggaaaggggctggagtgggttggccgt actcgaaataaagctaacggttacagtaca gagtatgccgcgtctgtgaaaggcagattcaccgtctcaagagatgactcagagaacttagtgcatctgcaaatgaacagcctgaaaagcgaggacacggccctgtattactgt gctagactgcactacagtggtggctggtacggtctggacgtc ARLHYSGGWYGLDV IGHV3-72 IGHD6-19 IGHJ6 ggattcaccttcagtgaccactac atagactgggtccgccaggctccaggaaaggggctggagtgggttggccgt actcgaaataaagctaacggttacagtaca gagtatgccgcgtctgtgaaaggcagattcaccgtctcaagagatgactcagagaacttagtgcatctgcaaatgaacagcctgaaaagcgaggacacggccctgtattactgt gctagactgcactacagtggtggctggtacggtctggacgtc IGA1 17 219 12 5 6 10 6 7 4 2 8 3 JY8QFUQ01AZK7Y 4194 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 990 95.39 207/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 8 18 8.8.18 [1.17.38.11] CARAGYCNNGVCYSSYFDFW in-frame + ctctggattcaccttcagtagctactggatgcactgggtccgccaagctccggggaaggggctggtgtgggtctcacgtatgaatagtgatggcagtgacataaggtacgcggactccgtgaggggccgattcaccatctccagagacaacaccaagaacacgctgtatctacaaatgaacagtctgagagccgaggacacggctgtgtattactgtgcaagagcgggatattgtaataatggtgtatgctactccagttactttgacttctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcc NA IGA2 79 84 [302, 303] a138>g|t168>g,I56>M(+ + -)|g186>c|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>t,T65>I(- - -)|c198>g,S66>R(- - -)|a215>g,K72>R(+ + +)|g247>a,A83>T(- - -)|g267>a| a138>g| t168>g,I56>M(+ + -)|g186>c|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>t,T65>I(- - -)| c198>g,S66>R(- - -)|a215>g,K72>R(+ + +)|g247>a,A83>T(- - -)|g267>a| a320>c| 319 (320) 224 (225) 214 10 (11) 3 (4) 7 3 3 1 0 0 (1) 0 0 0 1 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 30 24 19 5 1 4 1 1 1 0 0 0 0 0 1 0 0 1 117 114 110 4 1 3 1 2 0 0 0 0 0 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccggggaaggggctggtgtgggtctcacgt atgaatagtgatggcagtgacata aggtacgcggactccgtgaggggccgattcaccatctccagagacaacaccaagaacacgctgtatctacaaatgaacagtctgagagccgaggacacggctgtgtattactgt gcaagagcgggatattgtaataatggtgtatgctactccagttactttgacttc ARAGYCNNGVCYSSYFDF IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccggggaaggggctggtgtgggtctcacgt atgaatagtgatggcagtgacata aggtacgcggactccgtgaggggccgattcaccatctccagagacaacaccaagaacacgctgtatctacaaatgaacagtctgagagccgaggacacggctgtgtattactgt gcaagagcgggatattgtaataatggtgtatgctactccagttactttgacttc IGA2 10 213 7 3 4 6 3 4 2 1 3 4 JY8QFUQ01AZQ2B 3110 productive Homsap IGHV4-4*02 F, or Homsap IGHV4-4*03 F or Homsap IGHV4-4*04 F or Homsap IGHV4-4*05 F (see comment) 662 77.98 170/218 nt NA Homsap IGHJ1*01 F (see comment) 170 80.77 42/52 nt Homsap IGHD2-8*02 F 3 9 7 12 9.7.12 [1.17.38.11] CAREDIVREGLDHW in-frame + Low V-REGION identity (77,98% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) tgtctggtggctccatcgccacttatcattggtggaattgggtccgccagacccccgggaagggactggagtggattggggaagtctattatagtcgacagactaattacaacccgtccctccagagtcgcgttgacatttccattgacagtcccaacggtcagttcaccctatatctgagagatgtgaccgtcgcggacacggccgtttattattgcgcgagagaagacattgttcgggagggcctcgaccactggggccagggcaccctggtcaccgtgtcctcagcatccccggaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [286] c75>g|a91>g,S31>A(- + -)|g92>c,S31>A(- + -)|g104>c,S35>T(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a109>c,N37>H(- - -)|c111>t,N37>H(- - -)|g119>a,S40>N(- - -)|c133>a,P45>T(+ + -)|a138>c|g147>a|a166>g,I56>V(+ - +)|c172>t,H58>Y(+ - -)|g187>c,G63>R(- - -)|g189>a,G63>R(- - -)|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>g,S64>Q(- - -)|c195>t|c198>t|a214>c,K72>Q(+ - -)|a225>c|c228>t|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|a234>t|a237>c|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|a245>g,K82>S(- - -)|g246>t,K82>S(- - -)|t247>c,S83>P(+ - -)|g252>c,K84>N(+ - -)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>t,N85>G(- - -)|t262>a,S88>T(+ - +)|g267>a|a268>t,K90>Y(- - -)|g270>t,K90>Y(- - -)|c276>a,S92>R(- - -)|t277>g,S93>D(- - -)|c278>a,S93>D(- - -)|c287>t,A96>V(+ - +)|g303>t|c309>t|t312>c| c75>g| a91>g,S31>A(- + -)|g92>c,S31>A(- + -)|g104>c,S35>T(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a109>c,N37>H(- - -)|c111>t,N37>H(- - -)| g119>a,S40>N(- - -)|c133>a,P45>T(+ + -)|a138>c|g147>a| a166>g,I56>V(+ - +)|c172>t,H58>Y(+ - -)|g187>c,G63>R(- - -)|g189>a,G63>R(- - -)|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>g,S64>Q(- - -)|c195>t| c198>t|a214>c,K72>Q(+ - -)|a225>c|c228>t|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|a234>t|a237>c|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|a245>g,K82>S(- - -)|g246>t,K82>S(- - -)|t247>c,S83>P(+ - -)|g252>c,K84>N(+ - -)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>t,N85>G(- - -)|t262>a,S88>T(+ - +)|g267>a|a268>t,K90>Y(- - -)|g270>t,K90>Y(- - -)|c276>a,S92>R(- - -)|t277>g,S93>D(- - -)|c278>a,S93>D(- - -)|c287>t,A96>V(+ - +)|g303>t|c309>t|t312>c| 320 226 178 48 13 35 6 7 8 2 6 4 4 1 4 2 3 1 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 27 20 7 0 7 1 1 1 0 1 0 1 0 2 0 0 0 51 51 47 4 2 2 0 2 0 0 1 1 0 0 0 0 0 0 30 21 13 8 1 7 1 2 2 0 1 0 0 0 1 1 0 0 117 114 86 28 9 19 4 2 5 2 3 3 3 1 1 0 3 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tgtct ggtggctccatcgccacttatcattgg tggaattgggtccgccagacccccgggaagggactggagtggattggggaa gtctattatagtcgacagact aattacaacccgtccctccagagtcgcgttgacatttccattgacagtcccaacggtcagttcaccctatatctgagagatgtgaccgtcgcggacacggccgtttattattgc gcgagagaagacattgttcgggagggcctcgaccac AREDIVREGLDH IGHV4-4 IGHD2-8 IGHJ1 ggtggctccatcgccacttatcattgg tggaattgggtccgccagacccccgggaagggactggagtggattggggaa gtctattatagtcgacagact aattacaacccgtccctccagagtcgcgttgacatttccattgacagtcccaacggtcagttcaccctatatctgagagatgtgaccgtcgcggacacggccgtttattattgc gcgagagaagacattgttcgggagggcctcgaccac IGA1 47 213 23 24 15 27 8 20 11 1 21 14 JY8QFUQ01AZRH6 20749 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 959 93.58 204/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 3 8 8 13 8.8.13 [1.17.38.11] CARGLAVILGGFDYW in-frame - cctctggattcaccttcagtagttatagcatgcactgggtccgccaggctccagggaagggactggagtgggtctcctccattagtagtaatagtgcctacacagaccacgcagactcagtgaagggccgattcaccatctccagagacaacgacaagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagggctagcagtgattcttggaggatttgactactggggccagggaaacctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG2 100 100 [289] c108>t|a118>c,N40>H(- - -)|g147>a|a162>c|g176>a,S59>N(- - -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>c,S63>A(- + -)|t194>c,I65>T(- - -)|t196>g,Y66>D(- - -)|t199>c,Y67>H(+ - -)|c248>a,A83>D(- - -)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)| c108>t| a118>c,N40>H(- - -)|g147>a|a162>c| g176>a,S59>N(- - -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>c,S63>A(- + -)|t194>c,I65>T(- - -)| t196>g,Y66>D(- - -)|t199>c,Y67>H(+ - -)|c248>a,A83>D(- - -)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)| a320>g| 319 (320) 225 (226) 211 14 (15) 3 (4) 11 2 (3) 2 1 3 2 1 0 0 1 1 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 0 0 2 0 0 0 0 0 0 0 30 24 19 5 0 5 1 1 0 2 0 0 0 0 1 0 0 0 117 114 109 5 0 5 1 0 0 1 0 1 0 0 0 1 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtagttatagc atgcactgggtccgccaggctccagggaagggactggagtgggtctcctcc attagtagtaatagtgcctacaca gaccacgcagactcagtgaagggccgattcaccatctccagagacaacgacaagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggctagcagtgattcttggaggatttgactac ARGLAVILGGFDY IGHV3-21 IGHD3-22 IGHJ4 ggattcaccttcagtagttatagc atgcactgggtccgccaggctccagggaagggactggagtgggtctcctcc attagtagtaatagtgcctacaca gaccacgcagactcagtgaagggccgattcaccatctccagagacaacgacaagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggctagcagtgattcttggaggatttgactac IGG2 14 213 8 6 3 6 5 8 2 1 6 5 JY8QFUQ01AZRKU 17822 productive (see comment) Homsap IGHV3-7*03 F 850 88.43 191/216 nt 87.96 190/216 nt Homsap IGHJ6*03 F (see comment) 183 84.31 43/51 nt Homsap IGHD6-6*01 F 3 7 8 8 7.8.8 [1.17.38.11] CTRRGFMDVW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT, from codon 29 of V-REGION: 1 nucleotides (from position 12 in the user submitted sequence), (cause frameshift) ctccggattcattttggtgactattggatgagctgggtccgccaggttccagggaagggcctggagtgggtggccactacaaacgaggacgaaagtgacaagcggtatgtggactctgtgaagggccgcttcaccatctccagagacaacgccaagaactcactgtatttgcaaatgaacagcctgagaggccaggacgcggccgtgtattactgtaccagaagaggattcatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [271] t78>c|c87>t|a103>g,S35>G(+ + -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c134>t,A45>V(+ - +)|g147>c|a164>c,N55>T(- + -)|c165>t,N55>T(- + -)|t167>c,I56>T(- - -)|g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a174>g,Q58>E(+ + -)|t177>c|g185>a,G62>E(- - -)|g192>c,E64>D(+ - +)|a195>g|t196>c,Y66>R(- - -)|a197>g,Y66>R(- - -)|c198>g,Y66>R(- - -)|a225>c|c265>t|c287>g,A96>G(- + -)|g289>c,E97>Q(+ + -)|a295>g,T99>A(- - -)|g313>a,A105>T(- - -)|g315>c,A105>T(- - -)| t78>c| c87>t|a103>g,S35>G(+ + -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| c134>t,A45>V(+ - +)|g147>c|a164>c,N55>T(- + -)|c165>t,N55>T(- + -)| t167>c,I56>T(- - -)|g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a174>g,Q58>E(+ + -)|t177>c|g185>a,G62>E(- - -)|g192>c,E64>D(+ - +)|a195>g| t196>c,Y66>R(- - -)|a197>g,Y66>R(- - -)|c198>g,Y66>R(- - -)|a225>c|c265>t|c287>g,A96>G(- + -)|g289>c,E97>Q(+ + -)|a295>g,T99>A(- - -)| g313>a,A105>T(- - -)|g315>c,A105>T(- - -)|g319>a|a320>g| 318 (320) 222 (224) 195 27 (29) 7 (9) 20 6 (7) 3 (4) 4 4 2 0 0 0 5 3 0 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 23 19 4 1 3 2 1 1 0 0 0 0 0 0 0 0 0 51 51 47 4 1 3 0 0 2 0 1 0 0 0 1 0 0 0 30 24 16 8 2 6 2 1 0 2 0 0 0 0 2 1 0 0 117 114 106 8 2 6 2 0 1 1 1 0 0 0 1 2 0 0 6 (8) 6 4 2 (4) 0 (2) 2 0 (1) 1 (2) 0 0 0 0 0 0 1 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcattttggtgactattgg atgagctgggtccgccaggttccagggaagggcctggagtgggtggccact acaaacgaggacgaaagtgacaag cggtatgtggactctgtgaagggccgcttcaccatctccagagacaacgccaagaactcactgtatttgcaaatgaacagcctgagaggccaggacgcggccgtgtattactgt accagaagaggattcatggacgtc TRRGFMDV IGHV3-7 IGHD6-6 IGHJ6 ggattcattttggtgactattgg atgagctgggtccgccaggttccagggaagggcctggagtgggtggccact acaaacgaggacgaaagtgacaag cggtatgtggactctgtgaagggccgcttcaccatctccagagacaacgccaagaactcactgtatttgcaaatgaacagcctgagaggccaggacgcggccgtgtattactgt accagaagaggattcatggacgtc IGA2 24 212 15 9 6 13 9 11 3 3 9 9 JY8QFUQ01AZTNG 20622 productive Homsap IGHV3-49*03 F, or Homsap IGHV3-49*05 F 1110 100 223/223 nt NA Homsap IGHJ4*02 F 114 70.83 34/48 nt Homsap IGHD4-17*01 F 3 8 10 12 8.10.12 [1.17.38.11] CTKCSVTIYFLTTG in-frame + ttctggattcacctttggtgattatgctatgagctggttccgccaggctccagggaaggggctggagtgggtaggtttcattagaagcaaagcttatggtgggacaacagaatacgccgcgtctgtgaaaggcagattcaccatctcaagagatgattccaaaagcatcgcctatctgcaaatgaacagcctgaaaaccgaggacacagccgtgtattactgtactaagtgttcggtgactatttactttttgactactggggccagggtaaccctggtcaccgtctcctcagcttccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgcaaccggtgacggtgtcgtggaaactc NA IGG1 93 100 [291] g317>a,R106>K(+ + +)|a318>g,R106>K(+ + +)|g319>t|a320>g| 316 (320) 227 (231) 227 0 (4) 0 (2) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 0 0 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,158-159(FR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,181-182(CDR2)|aa,192-193(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|ta,69-70(FR1)|tt,75-76(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,124-125(FR2)|ta,158-159(FR2)|tt,162-163(FR2)|tt,167-168(CDR2)|tt,180-181(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agct,72-75(FR1)|agct,118-121(FR2)|ggtt,122-125(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggta,156-159(FR2)|ggtt,160-163(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|ggca,220-223(FR3)|agca,253-256(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,72-75(FR1)|tgct,111-114(CDR1)|agct,118-121(FR2)|agct,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| ttct ggattcacctttggtgattatgct atgagctggttccgccaggctccagggaaggggctggagtgggtaggtttc attagaagcaaagcttatggtgggacaaca gaatacgccgcgtctgtgaaaggcagattcaccatctcaagagatgattccaaaagcatcgcctatctgcaaatgaacagcctgaaaaccgaggacacagccgtgtattactgt actaagtgttcggtgactatttactttttgactact TKCSVTIYFLTT IGHV3-49 IGHD4-17 IGHJ4 ggattcacctttggtgattatgct atgagctggttccgccaggctccagggaaggggctggagtgggtaggtttc attagaagcaaagcttatggtgggacaaca gaatacgccgcgtctgtgaaaggcagattcaccatctcaagagatgattccaaaagcatcgcctatctgcaaatgaacagcctgaaaaccgaggacacagccgtgtattactgt actaagtgttcggtgactatttactttttgactact IGG1 0 219 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01AZU0Q 16285 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F (see comment) 705 81.31 174/214 nt 80.37 172/214 nt Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD6-6*01 F 3 8 7 9 8.7.9 [1.17.38.11] CARLDGHFDLW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (81,31% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR2-IMGT at codon 40 of V-REGION: 'C' (1 nucleotides at position 34 to 34 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 47 of V-REGION: 'C' (1 nucleotides at position 56 to 56 in the user submitted sequence), (cause frameshift); ctctgggttcaccatcagtcactactccatgggCctgggtccgccaggctccgggCaaaggggctggagtgggtctcattaatttatcccggcgggagcacattccacgcagactccgtgagggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgtgcgagactcgacgggcacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [275] g88>a,V30>I(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)|a118>g,S40>G(+ + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g|a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c222>g|a225>g|t226>c,F76>L(+ - -)|c228>t,F76>L(+ - -)|c230>t,T77>I(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| g88>a,V30>I(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)| a118>g,S40>G(+ + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g| a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c222>g|a225>g|t226>c,F76>L(+ - -)|c228>t,F76>L(+ - -)|c230>t,T77>I(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| g319>c|a320>t| 318 (320) 220 (222) 180 40 (42) 9 (11) 31 9 8 5 3 3 2 2 (3) 2 1 (2) 3 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 2 0 0 2 0 1 0 0 0 0 0 51 51 46 5 2 3 2 1 0 0 0 0 0 1 0 0 1 0 30 21 17 4 2 2 0 0 0 1 1 0 0 0 1 0 0 1 117 114 88 26 5 21 7 5 5 2 0 2 1 1 0 3 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcaccatcagtcactactcc atgggctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgagggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc ARLDGHFDL IGHV3-53 IGHD6-6 IGHJ2 gggttcaccatcagtcactactcc atgggctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgagggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc IGG2 40 210 25 15 13 20 12 20 7 2 24 7 JY8QFUQ01AZUTN 21962 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 981 94.93 206/217 nt NA Homsap IGHJ2*01 F 229 92.45 49/53 nt Homsap IGHD3-9*01 F 2 8 8 18 8.8.18 [1.17.38.11] CVKVAGDYRTPNYGWHFDLW in-frame - ctctggattcacctttagcacctatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtctcagttattagtggtagtggtggcagcacattctactcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacactggatctgcaaatgaatagcctgagaggcgaagacacggccgcatattactgtgtgaaagtggccggtgactatagaactcccaactacggctggcacttcgatctctggggccgaggcaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [302] g107>c,S36>T(+ - +)|c164>t,A55>V(+ - +)|t189>c|a197>t,Y66>F(- + -)|g202>t,A68>S(- + -)|g258>a|t262>g,Y88>D(- - -)|c276>t|c287>g,A96>G(- + -)|g291>a|t302>c,V101>A(+ - +)|c314>t,A105>V(+ - +)| g107>c,S36>T(+ - +)| c164>t,A55>V(+ - +)| t189>c| a197>t,Y66>F(- + -)|g202>t,A68>S(- + -)|g258>a|t262>g,Y88>D(- - -)|c276>t|c287>g,A96>G(- + -)|g291>a|t302>c,V101>A(+ - +)| c314>t,A105>V(+ - +)|a320>t| 319 (320) 224 (225) 212 12 (13) 4 (5) 8 0 2 3 2 0 0 1 (2) 0 1 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 106 8 3 5 0 2 1 1 0 0 1 0 0 1 1 1 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcacctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagtt attagtggtagtggtggcagcaca ttctactcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacactggatctgcaaatgaatagcctgagaggcgaagacacggccgcatattactgt gtgaaagtggccggtgactatagaactcccaactacggctggcacttcgatctc VKVAGDYRTPNYGWHFDL IGHV3-23 IGHD3-9 IGHJ2 ggattcacctttagcacctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagtt attagtggtagtggtggcagcaca ttctactcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacactggatctgcaaatgaatagcctgagaggcgaagacacggccgcatattactgt gtgaaagtggccggtgactatagaactcccaactacggctggcacttcgatctc IGA2 11 213 6 5 4 7 2 4 3 1 6 1 JY8QFUQ01AZZ31 4930 productive Homsap IGHV4-61*03 F 888 89.55 197/220 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD5-12*01 F 1 10 7 12 10.7.12 [1.17.38.11] CTRHPKSNVEGDSW in-frame + ccctggtggctccgtcagcagtggtagttatttctggagctggatccggcagcccccagggcaggggctggaatggatcggttatgtctataacaatgggaacaccaattacaacccttccctcaagagtcgagtcaccatttctatagacacgtccgagaatcacgtctccctgaagctggcctctgtgaccgctgcggacacggccgtgtactactgtacgaggcacccgaagtctaatgtagagggtgactcctggggccagggagccccggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttct NA IGA1 100 100 [287] t76>c,S26>P(+ - -)|c111>t|a113>t,Y38>F(- + -)|a142>c,K48>Q(+ - -)|a147>g|g153>a|t159>c|g162>t|a166>g,I56>V(+ - +)|t172>a,Y58>N(- - -)|g176>a,S59>N(- - -)|g191>a,S64>N(- - -)|c198>t|c207>t|a234>t|a237>t|g238>a,V80>I(+ - +)|a250>g,K84>E(+ - -)|c255>t|t259>g,F87>V(+ - -)|a274>g,S92>A(- + -)|g275>c,S92>A(- + -)|t306>c|g313>a,A105>T(- - -)| t76>c,S26>P(+ - -)| c111>t|a113>t,Y38>F(- + -)| a142>c,K48>Q(+ - -)|a147>g|g153>a|t159>c|g162>t| a166>g,I56>V(+ - +)|t172>a,Y58>N(- - -)|g176>a,S59>N(- - -)|g191>a,S64>N(- - -)| c198>t|c207>t|a234>t|a237>t|g238>a,V80>I(+ - +)|a250>g,K84>E(+ - -)|c255>t|t259>g,F87>V(+ - -)|a274>g,S92>A(- + -)|g275>c,S92>A(- + -)|t306>c| g313>a,A105>T(- - -)|a318>g|g319>c| 317 (320) 225 (228) 201 (202) 24 (26) 11 (13) 13 4 (5) 5 4 3 1 0 3 1 1 (2) 0 1 1 78 4 3 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 36 30 28 2 1 1 0 0 1 0 0 0 1 0 0 0 0 0 51 51 46 5 4 1 1 1 0 1 1 0 0 0 0 0 1 0 30 21 17 4 0 4 1 2 0 0 0 0 0 1 0 0 0 0 117 114 103 11 6 5 2 1 3 1 0 0 2 0 1 0 0 1 5 (8) 5 4 (5) 1 (3) 0 (2) 1 0 (1) 1 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ccct ggtggctccgtcagcagtggtagttatttc tggagctggatccggcagcccccagggcaggggctggaatggatcggttat gtctataacaatgggaacacc aattacaacccttccctcaagagtcgagtcaccatttctatagacacgtccgagaatcacgtctccctgaagctggcctctgtgaccgctgcggacacggccgtgtactactgt acgaggcacccgaagtctaatgtagagggtgactcc TRHPKSNVEGDS IGHV4-61 IGHD5-12 IGHJ4 ggtggctccgtcagcagtggtagttatttc tggagctggatccggcagcccccagggcaggggctggaatggatcggttat gtctataacaatgggaacacc aattacaacccttccctcaagagtcgagtcaccatttctatagacacgtccgagaatcacgtctccctgaagctggcctctgtgaccgctgcggacacggccgtgtactactgt acgaggcacccgaagtctaatgtagagggtgactcc IGA1 22 216 14 8 8 10 6 12 10 1 6 5 JY8QFUQ01B00R6 8703 productive Homsap IGHV3-11*01 F 855 88.48 192/217 nt NA Homsap IGHJ2*01 F 220 90.57 48/53 nt Homsap IGHD2-8*02 F 3 8 8 12 8.8.12 [1.17.38.11] CVDIRLGDWFFDLW in-frame + ctctggattcaccttcagtgaccactacatgagctgggtccgccaggctccggggaagggtctggaatggatctcatatatcagtacaagtggtaatatggtttattacgcggactctgtgaagggccgattcaccgtctccagggacaacaccaagagctcactgtatctgcaaatgaacggcctcagagtcgaggacacggccgtctattactgtgtcgacataagactgggagattggttcttcgatctctggggccgtggcaccctggtaactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [284] t109>c,Y37>H(+ - -)|a124>g,I42>V(+ - +)|a138>g|g147>t|g153>a|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|c165>t|t168>c|g173>c,S58>T(+ - +)|t174>a,S58>T(+ - +)|g188>a,S63>N(- - -)|c191>t,T64>M(- - -)|c192>g,T64>M(- - -)|a193>g,I65>V(+ - +)|a195>t,I65>V(+ - +)|c198>t|a204>g|a232>g,I78>V(+ - +)|g247>a,A83>T(- - -)|a254>g,N85>S(- - -)|a277>g,S93>G(+ + -)|g282>c|c287>t,A96>V(+ - +)|g303>c| t109>c,Y37>H(+ - -)| a124>g,I42>V(+ - +)|a138>g|g147>t|g153>a|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|c165>t| t168>c|g173>c,S58>T(+ - +)|t174>a,S58>T(+ - +)|g188>a,S63>N(- - -)|c191>t,T64>M(- - -)|c192>g,T64>M(- - -)|a193>g,I65>V(+ - +)|a195>t,I65>V(+ - +)| c198>t|a204>g|a232>g,I78>V(+ - +)|g247>a,A83>T(- - -)|a254>g,N85>S(- - -)|a277>g,S93>G(+ + -)|g282>c|c287>t,A96>V(+ - +)|g303>c| c314>t,A105>V(+ - +)|g315>c,A105>V(+ - +)|a316>g,R106>D(+ - -)|g317>a,R106>D(+ - -)|a318>c,R106>D(+ - -)|g319>a|a320>t| 313 (320) 218 (225) 193 25 (32) 9 (11) 16 (21) 7 (8) 4 (6) 4 (5) 3 0 (1) 0 1 (2) 1 3 (4) 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 51 51 44 7 4 3 2 2 1 1 0 0 0 0 0 0 1 0 30 24 16 8 1 7 1 1 1 1 0 0 1 1 1 1 0 0 117 114 105 9 4 5 4 1 2 0 0 0 0 0 2 0 0 0 1 (8) 1 1 0 (7) 0 (2) 0 (5) 0 (1) 0 (2) 0 (1) 0 0 (1) 0 0 (1) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgaccactac atgagctgggtccgccaggctccggggaagggtctggaatggatctcatat atcagtacaagtggtaatatggtt tattacgcggactctgtgaagggccgattcaccgtctccagggacaacaccaagagctcactgtatctgcaaatgaacggcctcagagtcgaggacacggccgtctattactgt gtcgacataagactgggagattggttcttcgatctc VDIRLGDWFFDL IGHV3-11 IGHD2-8 IGHJ2 ggattcaccttcagtgaccactac atgagctgggtccgccaggctccggggaagggtctggaatggatctcatat atcagtacaagtggtaatatggtt tattacgcggactctgtgaagggccgattcaccgtctccagggacaacaccaagagctcactgtatctgcaaatgaacggcctcagagtcgaggacacggccgtctattactgt gtcgacataagactgggagattggttcttcgatctc IGA1 25 213 18 7 8 13 10 12 8 1 8 8 JY8QFUQ01B02KX 12848 productive (see comment) Homsap IGHV3-48*01 F 1080 100 217/217 nt 99.54 216/217 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-2*01 F 2 8 8 10 8.8.10 [1.16.38.11] CASRLRSSGGYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 59 in the user submitted sequence), (cause frameshift) cctctggattcaccttcagtagctatagcatgaactgggtccgccaggctccagggaagggctggagtgggtttcatacattagtagtagtagtagtaccatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagccgcctacgtagttccggtgggtactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [279] a318>c,R106>S(- - -)|g319>c|a320>g| 317 (320) 222 (225) 222 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 50 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaagggctggagtgggtttcatac attagtagtagtagtagtaccata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagccgcctacgtagttccggtgggtac ASRLRSSGGY IGHV3-48 IGHD2-2 IGHJ4 ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaagggctggagtgggtttcatac attagtagtagtagtagtaccata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagccgcctacgtagttccggtgggtac IGG2 0 212 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01B03TR 15560 productive (see comment) Homsap IGHV3-48*01 F, or Homsap IGHV3-48*02 F 1032 96.82 213/220 nt 96.36 212/220 nt Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 164 81.63 40/49 nt Homsap IGHD2-21*02 F 3 8 8 7 8.8.7 [2.17.38.11] CARMTDDFW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 and Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) in CDR2-IMGT at codon 65 of V-REGION: 'C' (1 nucleotides at position 106 to 106 in the user submitted sequence), (cause frameshift); agtctctggattcaccttcagtagctatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagtagtagcagtagcaccatCaaaatacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtctattactgtgcgagaatgacagacgacttctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [273] c74>t,A25>V(+ - +)|t177>c|t189>c|t196>a,Y66>K(- - -)|c198>a,Y66>K(- - -)|c287>t,A96>V(+ - +)|g303>c| c74>t,A25>V(+ - +)| t177>c|t189>c| t196>a,Y66>K(- - -)|c198>a,Y66>K(- - -)|c287>t,A96>V(+ - +)|g303>c| g319>a|a320>t| 318 (320) 226 (228) 219 7 (9) 3 (5) 4 0 0 (1) 2 2 0 1 0 (1) 1 1 0 0 0 78 7 6 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 2 0 0 0 0 2 0 0 0 0 0 0 0 0 117 114 110 4 1 3 0 0 1 0 0 1 0 1 1 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agtctct ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtagtagcagtagcaccata aaatacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtctattactgt gcgagaatgacagacgacttc ARMTDDF IGHV3-48 IGHD2-21 IGHJ5 ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtagtagcagtagcaccata aaatacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtctattactgt gcgagaatgacagacgacttc IGA1 6 213 3 3 1 3 2 3 1 2 3 0 JY8QFUQ01B07O4 10183 productive Homsap IGHV3-7*01 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 114 70.83 34/48 nt Homsap IGHD4-17*01 F 3 8 8 11 8.8.11 [1.17.38.11] CARTSSREITDYW in-frame + ctctggattcacctttggtagctattggatggcctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagcaagatggaagtggtacatactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaattcactgtttctgcaaatggacagcctgagagtcgaggacacggctctgtattactgtgcgagaacctcgtcgcgggagattacggattactggggccagggaaccctggtcaccgtttgcctcagcatcccgaccagccccaaaggtcttcccgctgagcctctgcagcacccagccagatgggaaccgtggtcatcgcctgcctggtccagggcttcttcccccga NA IGA1 100 74 [282, 283] a103>g,S35>G(+ + -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a191>g,E64>G(- - -)|g192>t,E64>G(- - -)|a194>c,K65>T(- - -)|a232>g,I78>V(+ - +)|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)| a103>g,S35>G(+ + -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)| a191>g,E64>G(- - -)|g192>t,E64>G(- - -)|a194>c,K65>T(- - -)| a232>g,I78>V(+ - +)|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)| g319>a|a320>c| 318 (320) 223 (225) 211 12 (14) 1 (3) 11 5 0 (1) 2 0 1 (2) 0 1 0 2 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 30 24 21 3 0 3 1 0 0 0 1 0 0 0 0 0 1 0 117 114 108 6 1 5 2 0 2 0 0 0 1 0 1 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttggtagctattgg atggcctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaagtggtaca tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaattcactgtttctgcaaatggacagcctgagagtcgaggacacggctctgtattactgt gcgagaacctcgtcgcgggagattacggattac ARTSSREITDY IGHV3-7 IGHD4-17 IGHJ4 ggattcacctttggtagctattgg atggcctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaagtggtaca tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaattcactgtttctgcaaatggacagcctgagagtcgaggacacggctctgtattactgt gcgagaacctcgtcgcgggagattacggattac IGA1 12 213 7 5 2 5 5 7 1 0 7 4 JY8QFUQ01B08DF 17334 productive Homsap IGHV4-61*02 F 983 94.12 208/221 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-6*01 F 1 10 7 15 10.7.15 [1.17.38.11] CARNPSYSNSHQHCDYW in-frame + tctctggtgcctccatcaacagtggtagttactactggagttgggttcggcagcccgccgggaagggactggagtggattgggcgtatctacaccagtgggagcaccaactacaacccctccctcaagagtcgagtcgccatatcaatggacacgtccaagaaccagttctccctgaagctgaactctgtgaccgccgcagacacggccgtctatttttgtgcgagaaatccctcttatagcaattcgcaccagcattgtgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [297] g83>c,G28>A(- + -)|g92>a,S31>N(- - -)|c120>t|a124>g,I42>V(+ - +)|c126>t,I42>V(+ - +)|t171>c|a229>g,T77>A(- - -)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|g275>a,S92>N(- - -)|g303>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g83>c,G28>A(- + -)|g92>a,S31>N(- - -)| c120>t|a124>g,I42>V(+ - +)|c126>t,I42>V(+ - +)| t171>c| a229>g,T77>A(- - -)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|g275>a,S92>N(- - -)|g303>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g319>a| 318 (320) 227 (229) 214 (215) 13 (14) 3 (4) 10 3 3 (4) 3 1 0 0 1 0 2 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 51 51 48 3 1 2 1 0 2 0 0 0 0 0 0 0 0 0 30 21 20 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 107 7 1 6 2 2 1 0 0 0 1 0 1 0 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgcctccatcaacagtggtagttactac tggagttgggttcggcagcccgccgggaagggactggagtggattgggcgt atctacaccagtgggagcacc aactacaacccctccctcaagagtcgagtcgccatatcaatggacacgtccaagaaccagttctccctgaagctgaactctgtgaccgccgcagacacggccgtctatttttgt gcgagaaatccctcttatagcaattcgcaccagcattgtgactac ARNPSYSNSHQHCDY IGHV4-61 IGHD6-6 IGHJ4 ggtgcctccatcaacagtggtagttactac tggagttgggttcggcagcccgccgggaagggactggagtggattgggcgt atctacaccagtgggagcacc aactacaacccctccctcaagagtcgagtcgccatatcaatggacacgtccaagaaccagttctccctgaagctgaactctgtgaccgccgcagacacggccgtctatttttgt gcgagaaatccctcttatagcaattcgcaccagcattgtgactac IGA2 13 216 10 3 6 8 4 5 2 1 8 2 JY8QFUQ01B09U4 18217 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 959 93.12 203/218 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-3*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKIDSRNYDFWSGYYAHW in-frame - cctctggattcacctttaacagccatgccatgagttgggtccgccaggctccagggagggggctggagtgggtcgcagattctggtggcagtggtcgtaccacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccgagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtttattactgcgcgaaaatagatagtcggaattacgatttttggagtggttattatgcacactggggccagggatccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [301] g104>a,S35>N(- - -)|t109>c,Y37>H(+ - -)|c120>t|a143>g,K48>R(+ + +)|t160>g,S54>A(- + -)|c164>a,A55>D(- - -)|a166>t,I56>S(- - -)|t167>c,I56>S(- - -)|a169>g,S57>G(+ + -)|t174>c|g187>c,G63>R(- - -)|g191>c,S64>T(+ - +)|a250>g,K84>E(+ - -)|a303>t|t312>c| g104>a,S35>N(- - -)|t109>c,Y37>H(+ - -)| c120>t|a143>g,K48>R(+ + +)|t160>g,S54>A(- + -)|c164>a,A55>D(- - -)| a166>t,I56>S(- - -)|t167>c,I56>S(- - -)|a169>g,S57>G(+ + -)|t174>c|g187>c,G63>R(- - -)|g191>c,S64>T(+ - +)| a250>g,K84>E(+ - -)|a303>t|t312>c| g319>a|a320>t| 318 (320) 224 (226) 209 15 (17) 4 (6) 11 3 1 (2) 1 4 0 1 2 (3) 0 2 0 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 51 51 47 4 1 3 1 0 1 0 0 1 0 0 0 0 0 1 30 24 18 6 1 5 1 0 0 2 0 0 1 0 2 0 0 0 117 114 111 3 2 1 1 0 0 1 0 0 1 0 0 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttaacagccatgcc atgagttgggtccgccaggctccagggagggggctggagtgggtcgcagat tctggtggcagtggtcgtaccaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccgagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtttattactgc gcgaaaatagatagtcggaattacgatttttggagtggttattatgcacac AKIDSRNYDFWSGYYAH IGHV3-23 IGHD3-3 IGHJ4 ggattcacctttaacagccatgcc atgagttgggtccgccaggctccagggagggggctggagtgggtcgcagat tctggtggcagtggtcgtaccaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccgagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtttattactgc gcgaaaatagatagtcggaattacgatttttggagtggttattatgcacac IGG1 15 213 9 6 2 5 7 10 3 1 4 7 JY8QFUQ01B0B79 16390 productive (see comment) Homsap IGHV3-33*01 F, or Homsap IGHV3-33*05 F or Homsap IGHV3-33*06 F 798 88.61 179/202 nt 88.12 178/202 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD5-12*01 F 3 8 8 11 8.8.11 [1.17.33.11] CATEKPGNEFDYW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 84 of V-REGION: 15 nucleotides (from position 155 in the user submitted sequence), (do not cause frameshift) gtctggattcaccctcagttcctatgtcatacactgggtccgccaggctccaggcaaggggctggagtgggtggcagtcattggatttaatggacgcagcgagtattatgcagactccgtgaagggccgattcaccatctccagagacaattctctgcaaatgaacaatctgagagtcgaggacacggctgtctattactgtgcgactgagaaacccggcaacgaatttgattactggggccggggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggcccttgggctgcctggtcaaggactacttccccgaaccggtgacggtgtccgtggaactcag NA IGG2 100 100 [267] t88>c,F30>L(+ - -)|a106>t|g107>c|g113>t,G38>V(- - -)|g117>a,M39>I(+ + -)|t165>c|a168>t|t169>g,W57>G(- - -)|g171>a,W57>G(- - -)|a173>t,Y58>F(- + -)|g175>a,D59>N(+ + -)|a187>c,S63>R(- - -)|t189>c,S63>R(- - -)|a191>g,N64>S(- - -)|t192>c,N64>S(- - -)|a193>g,K65>E(+ - -)|a195>g,K65>E(+ - -)|c198>t|c249>t|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|c287>t,A96>V(+ - +)|g303>c| t88>c,F30>L(+ - -)|a106>t|g107>c|g113>t,G38>V(- - -)| g117>a,M39>I(+ + -)|t165>c| a168>t|t169>g,W57>G(- - -)|g171>a,W57>G(- - -)|a173>t,Y58>F(- + -)|g175>a,D59>N(+ + -)|a187>c,S63>R(- - -)|t189>c,S63>R(- - -)|a191>g,N64>S(- - -)|t192>c,N64>S(- - -)|a193>g,K65>E(+ - -)|a195>g,K65>E(+ - -)| c198>t|c249>t|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|c287>t,A96>V(+ - +)|g303>c| g317>c,R106>T(- - -)|a318>t,R106>T(- - -)| 316 (320) 206 (210) 183 (185) 23 (25) 7 16 (18) 3 4 4 4 1 0 3 (4) 0 2 (3) 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 0 0 1 0 0 1 0 1 0 1 0 51 51 49 2 1 1 0 1 0 1 0 0 0 0 0 0 0 0 30 24 13 11 1 10 3 2 0 2 1 0 2 0 0 0 0 1 117 99 93 6 3 3 0 1 4 0 0 0 0 0 1 0 0 0 4 (8) 4 4 (6) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcaccctcagttcctatgtc atacactgggtccgccaggctccaggcaaggggctggagtgggtggcagtc attggatttaatggacgcagcgag tattatgcagactccgtgaagggccgattcaccatctccagagacaattctctgcaaatgaacaatctgagagtcgaggacacggctgtctattactgt gcgactgagaaacccggcaacgaatttgattac ATEKPGNEFDY IGHV3-33 IGHD5-12 IGHJ4 ggattcaccctcagttcctatgtc atacactgggtccgccaggctccaggcaaggggctggagtgggtggcagtc attggatttaatggacgcagcgag tattatgcagactccgtgaagggccgattcaccatctccagagacaattctctgcaaatgaacaatctgagagtcgaggacacggctgtctattactgt gcgactgagaaacccggcaacgaatttgattac IGG2 23 198 15 8 8 11 7 12 4 3 4 12 JY8QFUQ01B0BDK 19760 productive Homsap IGHV3-11*01 F 887 89.91 196/218 nt NA Homsap IGHJ6*02 F 229 85.48 53/62 nt Homsap IGHD1-7*01 F 1 8 8 16 8.8.16 [1.17.38.11] CARGKGKGGTTYYGMDVW in-frame - cctctggattcactttcagtgactactacatgaattgggtccgccaggctccagggaagggcctggagtggctttcatacattactgcaagtggtgccatcatacactatgcagactctgtgaagggccgattcatcatctccagggacaacgccaagaactcactgtctctgcagatgaacagcctgagagccgacgacacggccgtctattattgtgctcgggggaagggcaagggtggaactacctactacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] c87>t|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a124>g,I42>V(+ - +)|g147>c|g157>c,V53>L(+ - +)|g170>c,S57>T(+ - +)|a172>g,S58>A(- + -)|g173>c,S58>A(- + -)|t174>a,S58>A(- + -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>c,S63>A(- + -)|c191>t,T64>I(- - -)|t196>c,Y66>H(+ - -)|c201>t|c230>t,T77>I(- - -)|a263>c,Y88>S(+ - -)|a270>g|g291>c,E97>D(+ - +)|g303>c|c309>t| c87>t| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a124>g,I42>V(+ - +)|g147>c|g157>c,V53>L(+ - +)| g170>c,S57>T(+ - +)|a172>g,S58>A(- + -)|g173>c,S58>A(- + -)|t174>a,S58>A(- + -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>c,S63>A(- + -)|c191>t,T64>I(- - -)| t196>c,Y66>H(+ - -)|c201>t|c230>t,T77>I(- - -)|a263>c,Y88>S(+ - -)|a270>g|g291>c,E97>D(+ - +)|g303>c|c309>t| g315>t|a316>c|a318>g|a320>g| 314 (320) 220 (226) 198 (200) 22 (26) 6 (10) 16 4 (6) 1 6 2 1 (2) 0 0 1 7 0 0 (1) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 46 5 1 4 1 1 1 0 0 0 0 0 2 0 0 0 30 24 16 8 0 8 2 0 1 1 0 0 0 1 3 0 0 0 117 114 106 8 4 4 1 0 3 1 1 0 0 0 2 0 0 0 2 (8) 2 2 (4) 0 (4) 0 (4) 0 0 (2) 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcactttcagtgactactac atgaattgggtccgccaggctccagggaagggcctggagtggctttcatac attactgcaagtggtgccatcata cactatgcagactctgtgaagggccgattcatcatctccagggacaacgccaagaactcactgtctctgcagatgaacagcctgagagccgacgacacggccgtctattattgt gctcgggggaagggcaagggtggaactacctactacggtatggacgtc ARGKGKGGTTYYGMDV IGHV3-11 IGHD1-7 IGHJ6 ggattcactttcagtgactactac atgaattgggtccgccaggctccagggaagggcctggagtggctttcatac attactgcaagtggtgccatcata cactatgcagactctgtgaagggccgattcatcatctccagggacaacgccaagaactcactgtctctgcagatgaacagcctgagagccgacgacacggccgtctattattgt gctcgggggaagggcaagggtggaactacctactacggtatggacgtc IGA1 22 213 13 9 7 14 6 8 5 1 8 8 JY8QFUQ01B0DOB 21285 productive Homsap IGHV3-48*02 F 963 94.01 204/217 nt NA Homsap IGHJ6*02 F 202 80.65 50/62 nt Homsap IGHD3-3*01 F 3 8 8 20 8.8.20 [1.17.38.11] CARARRTISGVVFIEHYGMDVW in-frame + ctctggattcaccttcaggacccatagcatgaactgggtccgccaggctccagggaaggggctggagtggatttcattcattagtagtagtagtggtaccatattttatgcagactctgtgaagggccggttcaccatctccagagacaatgccaagaactcactttatctgcaaatgaacagcctgagagacgaggatgcggctgtgtattactgtgcgagagcccgtaggacgatttctggagtggtttttattgagcactacggtatggacgtctggggcccagggaccacggtctccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 100 100 [308] t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|t109>c,Y37>H(+ - -)|g157>a,V53>I(+ - +)|a164>t,Y55>F(- + -)|a187>g,S63>G(+ + -)|a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|c201>t|a225>g|g261>t|c294>t|a295>g,T99>A(- - -)| t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|t109>c,Y37>H(+ - -)| g157>a,V53>I(+ - +)|a164>t,Y55>F(- + -)| a187>g,S63>G(+ + -)| a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|c201>t|a225>g|g261>t|c294>t|a295>g,T99>A(- - -)| a320>c| 319 (320) 224 (225) 211 13 (14) 4 (5) 9 3 1 3 1 0 (1) 0 2 0 1 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 1 0 0 0 0 1 0 0 1 51 51 49 2 0 2 0 1 0 0 0 0 1 0 0 0 0 0 30 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 117 114 107 7 4 3 2 0 3 0 0 0 1 0 0 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ctct ggattcaccttcaggacccatagc atgaactgggtccgccaggctccagggaaggggctggagtggatttcattc attagtagtagtagtggtaccata ttttatgcagactctgtgaagggccggttcaccatctccagagacaatgccaagaactcactttatctgcaaatgaacagcctgagagacgaggatgcggctgtgtattactgt gcgagagcccgtaggacgatttctggagtggtttttattgagcactacggtatggacgtc ARARRTISGVVFIEHYGMDV IGHV3-48 IGHD3-3 IGHJ6 ggattcaccttcaggacccatagc atgaactgggtccgccaggctccagggaaggggctggagtggatttcattc attagtagtagtagtggtaccata ttttatgcagactctgtgaagggccggttcaccatctccagagacaatgccaagaactcactttatctgcaaatgaacagcctgagagacgaggatgcggctgtgtattactgt gcgagagcccgtaggacgatttctggagtggtttttattgagcactacggtatggacgtc IGA2 13 213 8 5 4 6 4 7 4 0 5 4 JY8QFUQ01B0E0W 13784 productive (see comment) Homsap IGHV3-48*01 F, or Homsap IGHV3-48*02 F 999 95.85 208/217 nt 94.93 206/217 nt Homsap IGHJ6*02 F 256 90.32 56/62 nt Homsap IGHD4-23*01 ORF 2 8 8 20 8.8.20 [1.16.38.11] CVRGDYGGAPGHYYDPYGLDVW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 62 of V-REGION: 'G' (1 nucleotides at position 93 to 93 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 59 in the user submitted sequence), (cause frameshift) cctctggattcaccttcaggagttatatcatgaactgggtccgccaggctccagggaagggctggagtggatttcatacattagtagtagtgGgtattatcatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgtgtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtctggggccaagggaccacggtcaaagtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [310] t105>g,S35>R(- - -)|c108>t|g113>t,S38>I(- - -)|g157>a,V53>I(+ - +)|a184>g,S62>G(+ + -)|g188>t,S63>I(- - -)|c191>t,T64>I(- - -)|c287>t,A96>V(+ - +)|c309>t|c314>t,A105>V(+ - +)| t105>g,S35>R(- - -)|c108>t|g113>t,S38>I(- - -)| g157>a,V53>I(+ - +)| a184>g,S62>G(+ + -)|g188>t,S63>I(- - -)|c191>t,T64>I(- - -)| c287>t,A96>V(+ - +)|c309>t| c314>t,A105>V(+ - +)|a320>g| 319 (320) 224 (225) 214 10 (11) 2 (3) 8 1 (2) 1 5 0 0 0 0 0 0 0 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 1 0 0 0 0 0 0 0 1 1 51 50 49 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 1 0 0 0 0 0 0 0 1 0 117 114 112 2 1 1 0 0 2 0 0 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcaggagttatatc atgaactgggtccgccaggctccagggaagggctggagtggatttcatac attagtagtagtggtattatcata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgt gtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtc VRGDYGGAPGHYYDPYGLDV IGHV3-48 IGHD4-23 IGHJ6 ggattcaccttcaggagttatatc atgaactgggtccgccaggctccagggaagggctggagtggatttcatac attagtagtagtggtattatcata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgt gtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtc IGG1 9 212 6 3 5 7 1 2 1 1 2 5 JY8QFUQ01B0E54 7789 productive Homsap IGHV3-30*10 F (see comment) 724 81.94 177/216 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD1-26*01 F 1 8 8 11 8.8.11 [1.17.38.11] CLAEVGGRQFDNW in-frame + Low V-REGION identity (81,94% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tctggattcaccttcaggaatcatgctatgcactgggtccgccaggcaccaggcaagggactagagtgggtggcacgcatctcacatgatggtgttagcgagatgtatacagaccccgtgaggggccgattctccatctccagagacaattccaaaagtattctatctctgcaaattaatggcctgagaagtgacgacacggctgtgtattattgtttggccgaagtgggaggccgacaatttgacaactggggccagggaaccctggtcactgtgtcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [280] t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>c,Y37>H(+ - -)|t135>a|g147>a|g163>c,V55>R(- - -)|t164>g,V55>R(- - -)|t165>c,V55>R(- - -)|a168>c|t172>c,Y58>H(+ - -)|a186>t|a187>g,S63>V(- - -)|g188>t,S63>V(- - -)|a191>g,N64>S(- - -)|t192>c,N64>S(- - -)|a193>g,K65>E(+ - -)|a195>g,K65>E(+ - -)|t196>a,Y66>M(- - -)|a197>t,Y66>M(- - -)|c198>g,Y66>M(- - -)|c201>t|t208>c,S70>P(+ - -)|a215>g,K72>R(+ + +)|a229>t,T77>S(+ - +)|g252>a|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c257>t,T86>I(- - -)|g258>t,T86>I(- - -)|g261>a|a263>c,Y88>S(+ - -)|g273>t,M91>I(+ + -)|c276>t|a277>g,S93>G(+ + -)|g286>a,A96>S(- + -)|c287>g,A96>S(- + -)|g291>c,E97>D(+ - +)|c309>t| t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>c,Y37>H(+ - -)| t135>a|g147>a|g163>c,V55>R(- - -)|t164>g,V55>R(- - -)|t165>c,V55>R(- - -)| a168>c|t172>c,Y58>H(+ - -)|a186>t|a187>g,S63>V(- - -)|g188>t,S63>V(- - -)|a191>g,N64>S(- - -)|t192>c,N64>S(- - -)|a193>g,K65>E(+ - -)|a195>g,K65>E(+ - -)| t196>a,Y66>M(- - -)|a197>t,Y66>M(- - -)|c198>g,Y66>M(- - -)|c201>t|t208>c,S70>P(+ - -)|a215>g,K72>R(+ + +)|a229>t,T77>S(+ - +)|g252>a|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c257>t,T86>I(- - -)|g258>t,T86>I(- - -)|g261>a|a263>c,Y88>S(+ - -)|g273>t,M91>I(+ + -)|c276>t|a277>g,S93>G(+ + -)|g286>a,A96>S(- + -)|c287>g,A96>S(- + -)|g291>c,E97>D(+ - +)|c309>t| g313>t,A105>L(+ - +)|c314>t,A105>L(+ - +)|a316>g,R106>A(- - -)|g317>c,R106>A(- - -)|a318>c,R106>A(- - -)| 312 (320) 216 (224) 177 (180) 39 (44) 9 30 (35) 7 (8) 5 6 (7) 5 2 (3) 0 3 2 2 (3) 2 3 (4) 2 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 1 1 0 0 0 0 0 0 0 1 51 51 46 5 2 3 0 1 0 1 0 0 0 1 1 0 0 1 30 24 15 9 2 7 4 0 0 2 1 0 1 0 0 0 1 0 117 114 93 21 5 16 3 3 5 1 1 0 2 1 1 2 2 0 0 (8) 0 0 (3) 0 (5) 0 0 (5) 0 (1) 0 0 (1) 0 0 (1) 0 0 0 0 (1) 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcaggaatcatgct atgcactgggtccgccaggcaccaggcaagggactagagtgggtggcacgc atctcacatgatggtgttagcgag atgtatacagaccccgtgaggggccgattctccatctccagagacaattccaaaagtattctatctctgcaaattaatggcctgagaagtgacgacacggctgtgtattattgt ttggccgaagtgggaggccgacaatttgacaac LAEVGGRQFDN IGHV3-30 IGHD1-26 IGHJ4 ggattcaccttcaggaatcatgct atgcactgggtccgccaggcaccaggcaagggactagagtgggtggcacgc atctcacatgatggtgttagcgag atgtatacagaccccgtgaggggccgattctccatctccagagacaattccaaaagtattctatctctgcaaattaatggcctgagaagtgacgacacggctgtgtattattgt ttggccgaagtgggaggccgacaatttgacaac IGA1 39 213 23 16 11 18 12 21 7 2 19 11 JY8QFUQ01B0F0W 19369 productive Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 968 94.04 205/218 nt NA Homsap IGHJ3*02 F 124 72 36/50 nt Homsap IGHD2-8*01 F 3 8 8 10 8.8.10 [1.17.38.11] CAREVSDAFEMW in-frame + cctctggattcacctttgatgattttggcatgagttgggtccgccaagttccagggaaggggctggagtgggtcgctggtatcagttggaatggaggcaaaacaggtcacgcagactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgagagccgaggacacggccgtgtatcactgtgcgagagaggtgtctgatgcttttgaaatgtggggtcaagggacaacggtcattggtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [280] a110>t,Y37>F(- + -)|c120>t|c134>t,A45>V(+ - +)|t160>g,S54>A(- + -)|t168>c|a170>g,N57>S(- - -)|t186>a|t189>c|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|t199>c,Y67>H(+ - -)|t201>c,Y67>H(+ - -)|t301>g,L101>V(+ - +)| a110>t,Y37>F(- + -)| c120>t|c134>t,A45>V(+ - +)|t160>g,S54>A(- + -)| t168>c|a170>g,N57>S(- - -)|t186>a|t189>c|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)| t199>c,Y67>H(+ - -)|t201>c,Y67>H(+ - -)|t301>g,L101>V(+ - +)| 320 226 213 13 4 9 1 1 2 4 0 1 1 1 0 0 0 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 51 51 48 3 1 2 0 0 2 0 0 0 0 0 0 0 0 1 30 24 18 6 3 3 1 1 0 2 0 1 0 1 0 0 0 0 117 114 111 3 0 3 0 0 0 2 0 0 0 0 0 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| cctct ggattcacctttgatgattttggc atgagttgggtccgccaagttccagggaaggggctggagtgggtcgctggt atcagttggaatggaggcaaaaca ggtcacgcagactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgagagccgaggacacggccgtgtatcactgt gcgagagaggtgtctgatgcttttgaaatg AREVSDAFEM IGHV3-20 IGHD2-8 IGHJ3 ggattcacctttgatgattttggc atgagttgggtccgccaagttccagggaaggggctggagtgggtcgctggt atcagttggaatggaggcaaaaca ggtcacgcagactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgagagccgaggacacggccgtgtatcactgt gcgagagaggtgtctgatgcttttgaaatg IGA1 13 213 8 5 3 4 5 9 1 3 5 4 JY8QFUQ01B0GQK 20370 productive Homsap IGHV3-11*01 F 981 94.93 206/217 nt NA Homsap IGHJ4*02 F (see comment) 222 95.83 46/48 nt Homsap IGHD5-18*01 F 3 8 8 18 8.8.18 [1.17.38.11] CVRRVWGYSYGSVSYYFEYW in-frame + Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) ctctggattcaccttcagtgactactacatgaactggatccgccaggctccagggaaggggctggagtgggtttcatacattagtggcagtgctactaccatatcctacgcagactctgtgaagggccgattcaccatctccagggacaacgccaaaaactcactttatctgcaaatggacagcctgagagccgaggacacggccatatattactgtgtgaggcgggtgtggggatacagttatggttccgtttcgtactactttgaatactggggccaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [302] g119>a,S40>N(- - -)|a172>g,S58>G(+ + -)|t174>c,S58>G(+ + -)|g185>c,G62>A(- + -)|g188>c,S63>T(+ - +)|a197>c,Y66>S(+ - -)|g252>a|g261>t|a274>g,N92>D(+ + -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c314>t,A105>V(+ - +)| g119>a,S40>N(- - -)| a172>g,S58>G(+ + -)|t174>c,S58>G(+ + -)|g185>c,G62>A(- + -)|g188>c,S63>T(+ - +)| a197>c,Y66>S(+ - -)|g252>a|g261>t|a274>g,N92>D(+ + -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| c314>t,A105>V(+ - +)|a318>g|g319>c|a320>g| 317 (320) 222 (225) 210 12 (15) 2 (5) 10 2 (4) 4 1 1 1 0 0 0 2 (3) 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 0 1 0 0 0 0 2 0 0 0 117 114 108 6 2 4 1 3 0 0 1 0 0 0 0 0 1 0 5 (8) 5 4 1 (4) 0 (3) 1 0 (2) 0 1 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgactactac atgaactggatccgccaggctccagggaaggggctggagtgggtttcatac attagtggcagtgctactaccata tcctacgcagactctgtgaagggccgattcaccatctccagggacaacgccaaaaactcactttatctgcaaatggacagcctgagagccgaggacacggccatatattactgt gtgaggcgggtgtggggatacagttatggttccgtttcgtactactttgaatac VRRVWGYSYGSVSYYFEY IGHV3-11 IGHD5-18 IGHJ4 ggattcaccttcagtgactactac atgaactggatccgccaggctccagggaaggggctggagtgggtttcatac attagtggcagtgctactaccata tcctacgcagactctgtgaagggccgattcaccatctccagggacaacgccaaaaactcactttatctgcaaatggacagcctgagagccgaggacacggccatatattactgt gtgaggcgggtgtggggatacagttatggttccgtttcgtactactttgaatac IGA1 11 213 7 4 4 7 3 4 2 0 5 4 JY8QFUQ01B0JWX 13057 productive Homsap IGHV4-61*02 F (see comment) 677 78.73 174/221 nt NA Homsap IGHJ4*02 F (see comment) 177 85.42 41/48 nt Homsap IGHD3-16*01 F 3 10 7 16 10.7.16 [1.17.38.11] CARDRRRSIAGARHFDSW in-frame - Low V-REGION identity (78,73% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) tctctggtggccccaccatcgatgatggtgatttgtggacctggatccggcagcccgccggcaagggactggaatggatcggttatgtctatgatgggcggggcaccaactccaacccctccctccggagtcgcgttaccctgtcacaagacacctcaaacaatcagttcttcttgaggttgaagtctgtgaccgcctcagacacggccgtctattactgtgcgcgagaccgccgtaggtcgattgccggagcgagacactttgactcctggggccagggaatcctggtcaccgtcgccgcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [300] t85>c,S29>P(+ - -)|t89>c,I30>T(- - -)|g92>t,S31>I(- - -)|a100>g,S34>D(- - -)|g101>a,S34>D(- - -)|g104>a,G35>D(- - -)|a106>g,S36>G(+ + -)|t109>g,Y37>D(- - -)|c111>t,Y37>D(- - -)|a113>t,Y38>L(- - -)|c114>g,Y38>L(- - -)|g119>c,S40>T(+ - +)|g141>c|g153>a|t159>c|g162>t|c163>t,R55>Y(- - -)|g164>a,R55>Y(- - -)|a166>g,I56>V(+ - +)|a172>g,T58>D(- + -)|c173>a,T58>D(- + -)|c174>t,T58>D(- + -)|a175>g,S59>G(+ + -)|t177>g,S59>G(+ + -)|g187>c,G63>R(- - -)|a190>g,S64>G(+ + -)|a200>c,Y67>S(+ - -)|a214>c,K72>R(+ + +)|a215>g,K72>R(+ + +)|a225>c|c228>t|a232>c,I78>L(+ + +)|a234>g,I78>L(+ + +)|g238>c,V80>Q(- + -)|t239>a,V80>Q(- + -)|g246>c|c249>a|g252>c,K84>N(+ - -)|c255>t|c263>t,S88>F(- - -)|c265>t|a269>g,K90>R(+ + +)|c271>t|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g289>t,A97>S(- + -)|g303>c|a316>c| t85>c,S29>P(+ - -)|t89>c,I30>T(- - -)|g92>t,S31>I(- - -)|a100>g,S34>D(- - -)|g101>a,S34>D(- - -)|g104>a,G35>D(- - -)|a106>g,S36>G(+ + -)|t109>g,Y37>D(- - -)|c111>t,Y37>D(- - -)|a113>t,Y38>L(- - -)|c114>g,Y38>L(- - -)| g119>c,S40>T(+ - +)|g141>c|g153>a|t159>c|g162>t|c163>t,R55>Y(- - -)|g164>a,R55>Y(- - -)| a166>g,I56>V(+ - +)|a172>g,T58>D(- + -)|c173>a,T58>D(- + -)|c174>t,T58>D(- + -)|a175>g,S59>G(+ + -)|t177>g,S59>G(+ + -)|g187>c,G63>R(- - -)|a190>g,S64>G(+ + -)| a200>c,Y67>S(+ - -)|a214>c,K72>R(+ + +)|a215>g,K72>R(+ + +)|a225>c|c228>t|a232>c,I78>L(+ + +)|a234>g,I78>L(+ + +)|g238>c,V80>Q(- + -)|t239>a,V80>Q(- + -)|g246>c|c249>a|g252>c,K84>N(+ - -)|c255>t|c263>t,S88>F(- - -)|c265>t|a269>g,K90>R(+ + +)|c271>t|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g289>t,A97>S(- + -)|g303>c| a316>c| 320 229 181 48 13 35 9 5 8 3 5 2 1 1 7 2 3 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 19 11 0 11 2 2 1 2 0 0 1 0 0 1 1 1 51 51 44 7 4 3 0 2 1 1 0 0 0 0 2 0 1 0 30 21 13 8 0 8 4 0 1 0 0 1 0 0 1 0 0 1 117 114 93 21 8 13 3 1 5 0 4 1 0 1 4 1 1 0 8 8 7 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggccccaccatcgatgatggtgatttg tggacctggatccggcagcccgccggcaagggactggaatggatcggttat gtctatgatgggcggggcacc aactccaacccctccctccggagtcgcgttaccctgtcacaagacacctcaaacaatcagttcttcttgaggttgaagtctgtgaccgcctcagacacggccgtctattactgt gcgcgagaccgccgtaggtcgattgccggagcgagacactttgactcc ARDRRRSIAGARHFDS IGHV4-61 IGHD3-16 IGHJ4 ggtggccccaccatcgatgatggtgatttg tggacctggatccggcagcccgccggcaagggactggaatggatcggttat gtctatgatgggcggggcacc aactccaacccctccctccggagtcgcgttaccctgtcacaagacacctcaaacaatcagttcttcttgaggttgaagtctgtgaccgcctcagacacggccgtctattactgt gcgcgagaccgccgtaggtcgattgccggagcgagacactttgactcc IGA1 47 216 25 22 13 27 12 20 12 0 16 19 JY8QFUQ01B0K72 8273 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 890 90.7 195/215 nt 89.77 193/215 nt Homsap IGHJ5*02 F 228 94.12 48/51 nt Homsap IGHD4-17*01 F 2 8 7 15 8.7.15 [1.17.38.11] CAKINNDFGDYDWFDPW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 63 of V-REGION: 'G' (1 nucleotides at position 95 to 95 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 69 of V-REGION: 'C' (1 nucleotides at position 113 to 113 in the user submitted sequence), (cause frameshift); tctctggtgtctccatcagtagttcctactggagttggatccggcagcccccagggaagggcctggagtggattggttatgtctattatactggGgggcaccgactccaaccCcctccctcaagagtcgtgtcaccctgtcaatggacacgtccaagaaccagttctccttggaactgagctctgtgaccgctgcggacacggccgtctattactgtgcgaaaatcaacaatgacttcggtgactacgactggttcgacccctggggccagggaatgctggtcaccgtctcctcagcatccccgaccagcccctaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccaggggcttcttcccccag NA IGA1 100 89 [294, 293] g83>t,G28>V(- - -)|a110>c,Y37>S(+ - -)|c120>t|a147>c|g162>t|a166>g,I56>V(+ - +)|c174>t|g176>c,S59>T(+ - +)|a190>g,S64>G(+ + -)|a196>g,N66>D(+ + -)|a200>c,Y67>S(+ - -)|a225>t|a232>c,I78>L(+ + +)|a234>g,I78>L(+ + +)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|c265>t|a268>g,K90>E(+ - -)|g270>a,K90>E(+ - -)|g303>c| g83>t,G28>V(- - -)|a110>c,Y37>S(+ - -)| c120>t|a147>c|g162>t| a166>g,I56>V(+ - +)|c174>t|g176>c,S59>T(+ - +)|a190>g,S64>G(+ + -)| a196>g,N66>D(+ + -)|a200>c,Y67>S(+ - -)|a225>t|a232>c,I78>L(+ + +)|a234>g,I78>L(+ + +)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|c265>t|a268>g,K90>E(+ - -)|g270>a,K90>E(+ - -)|g303>c| g317>a,R106>K(+ + +)|g319>a|a320>t| 316 (320) 219 (223) 199 (200) 20 (23) 7 (9) 13 (14) 6 2 (4) 3 0 4 0 1 (2) 0 2 0 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 1 0 0 0 0 0 1 0 51 51 48 3 3 0 0 0 1 0 1 0 0 0 0 0 1 0 30 21 17 4 1 3 2 0 1 0 0 0 0 0 1 0 0 0 117 114 103 11 3 8 4 2 1 0 2 0 1 0 1 0 0 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 0 (2) 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgtctccatcagtagttcctac tggagttggatccggcagcccccagggaagggcctggagtggattggttat gtctattatactgggggcacc gactccaacccctccctcaagagtcgtgtcaccctgtcaatggacacgtccaagaaccagttctccttggaactgagctctgtgaccgctgcggacacggccgtctattactgt gcgaaaatcaacaatgacttcggtgactacgactggttcgacccc AKINNDFGDYDWFDP IGHV4-59 IGHD4-17 IGHJ5 ggtgtctccatcagtagttcctac tggagttggatccggcagcccccagggaagggcctggagtggattggttat gtctattatactgggggcacc gactccaacccctccctcaagagtcgtgtcaccctgtcaatggacacgtccaagaaccagttctccttggaactgagctctgtgaccgctgcggacacggccgtctattactgt gcgaaaatcaacaatgacttcggtgactacgactggttcgacccc IGA1 20 210 11 9 5 9 6 11 6 1 8 5 JY8QFUQ01B0M6T 16316 productive Homsap IGHV1-2*02 F 822 87.38 187/214 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD1-26*01 F 3 8 8 14 8.8.14 [0.17.38.11] CARCLWISGTYHFDFW in-frame + tggatatactttcaccgaccattatgtttactggatgcgacaggcccctggacaaggccttgagtggatgggatggattaaccctcaaagtggtggcacaaactatgcactgaaatttcagggcagggtcaccatgaccacagacacgtccaccagcacagtgtacatggatctgagtggactgaattctgacgacacgggcgtgtattactgtgcgcgatgtctctggattagtgggacctatcactttgatttctggggccagggaaccctagtcaccgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] c84>t|c87>t|g107>a,G36>D(- - -)|t109>c,Y37>H(+ - -)|c111>t,Y37>H(+ - -)|a115>g,M39>V(+ - -)|g117>t,M39>V(+ - -)|c118>t,H40>Y(+ - -)|g124>a,V42>M(+ - -)|g147>c|c168>t|a175>c,N59>Q(+ - +)|c177>a,N59>Q(+ - +)|a206>t,Q69>L(- - -)|g210>a|g239>c,R80>T(- - -)|g240>a,R80>T(- - -)|t251>c,I84>T(- - -)|c260>t,A87>V(+ - +)|c261>g,A87>V(+ - +)|g270>t,E90>D(+ - +)|c276>t|a277>g,R93>G(- - -)|g279>a,R93>G(- - -)|g284>a,R95>N(+ - -)|a285>t,R95>N(+ - -)|c299>g,A100>G(- + -)|a316>c| c84>t|c87>t|g107>a,G36>D(- - -)|t109>c,Y37>H(+ - -)|c111>t,Y37>H(+ - -)| a115>g,M39>V(+ - -)|g117>t,M39>V(+ - -)|c118>t,H40>Y(+ - -)|g124>a,V42>M(+ - -)|g147>c| c168>t|a175>c,N59>Q(+ - +)|c177>a,N59>Q(+ - +)| a206>t,Q69>L(- - -)|g210>a|g239>c,R80>T(- - -)|g240>a,R80>T(- - -)|t251>c,I84>T(- - -)|c260>t,A87>V(+ - +)|c261>g,A87>V(+ - +)|g270>t,E90>D(+ - +)|c276>t|a277>g,R93>G(- - -)|g279>a,R93>G(- - -)|g284>a,R95>N(+ - -)|a285>t,R95>N(+ - -)|c299>g,A100>G(- + -)| a316>c|g319>t|a320>g| 318 (320) 220 (222) 192 28 (30) 7 (9) 21 2 (3) 6 7 2 2 1 2 0 2 2 2 (3) 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 1 3 1 0 0 0 0 0 0 0 0 51 51 46 5 1 4 1 1 1 0 0 0 0 0 1 0 1 0 30 24 21 3 1 2 0 0 1 0 1 1 0 0 0 0 0 0 117 114 100 14 2 12 1 4 2 1 0 0 2 0 1 2 1 0 6 (8) 6 5 1 (3) 1 (3) 0 0 (1) 0 0 0 1 0 0 0 0 0 0 (1) 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatatactttcaccgaccattat gtttactggatgcgacaggcccctggacaaggccttgagtggatgggatgg attaaccctcaaagtggtggcaca aactatgcactgaaatttcagggcagggtcaccatgaccacagacacgtccaccagcacagtgtacatggatctgagtggactgaattctgacgacacgggcgtgtattactgt gcgcgatgtctctggattagtgggacctatcactttgatttc ARCLWISGTYHFDF IGHV1-2 IGHD1-26 IGHJ4 ggatatactttcaccgaccattat gtttactggatgcgacaggcccctggacaaggccttgagtggatgggatgg attaaccctcaaagtggtggcaca aactatgcactgaaatttcagggcagggtcaccatgaccacagacacgtccaccagcacagtgtacatggatctgagtggactgaattctgacgacacgggcgtgtattactgt gcgcgatgtctctggattagtgggacctatcactttgatttc IGA1 27 213 17 10 13 20 4 7 3 3 16 5 JY8QFUQ01B0MEN 11068 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 1003 96.3 208/216 nt 95.83 207/216 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 7 8 8.7.8 [1.17.38.11] CARDNYGDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 63 of V-REGION: 1 nucleotides (from position 95 in the user submitted sequence), (cause frameshift) ctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtgttaatggtgatggggtagcacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtcc NA IGA2 100 100 [271] a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| 320 224 216 8 4 4 3 1 0 0 0 0 0 0 3 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 23 21 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 4 2 1 1 0 0 0 0 0 0 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac IGA2 8 212 4 4 1 5 3 3 4 0 2 2 JY8QFUQ01B0SLO 16095 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 930 92.99 199/214 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD6-25*01 F 1 8 8 15 8.8.15 [0.17.38.11] CARHEGYNSRYYYFGYW in-frame + tggatacagctttgccaccttctggatcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggaatgatctttcctggtgactccgataccagatacagcccgtccttccaaggccaggtcaccttctcagccgacaagtccatcaacaccgcctacctgcagttgaacagcctgacggcctcagacaccgccgtttattactgtgcgagacatgaaggatataacagccgttactactactttggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [290] a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|g162>a|c165>g,I55>M(+ + -)|a170>t,Y57>F(- + -)|t189>c|a232>t,I78>F(+ - -)|g254>a,S85>N(- - -)|g272>t,W91>L(+ - -)|g275>a,S92>N(- - -)|a284>c,K95>T(- - -)|g291>a|a301>g,M101>V(+ - -)|g303>t,M101>V(+ - -)| a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)| g162>a|c165>g,I55>M(+ + -)| a170>t,Y57>F(- + -)|t189>c| a232>t,I78>F(+ - -)|g254>a,S85>N(- - -)|g272>t,W91>L(+ - -)|g275>a,S92>N(- - -)|a284>c,K95>T(- - -)|g291>a|a301>g,M101>V(+ - -)|g303>t,M101>V(+ - -)| 320 222 207 15 3 12 2 4 0 1 1 0 3 0 1 1 2 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 0 0 0 0 0 1 0 1 0 0 0 51 51 49 2 1 1 0 1 0 0 0 0 0 0 0 1 0 0 30 24 22 2 1 1 0 0 0 1 0 0 1 0 0 0 0 0 117 114 106 8 1 7 1 3 0 0 1 0 1 0 0 0 2 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacagctttgccaccttctgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggaatg atctttcctggtgactccgatacc agatacagcccgtccttccaaggccaggtcaccttctcagccgacaagtccatcaacaccgcctacctgcagttgaacagcctgacggcctcagacaccgccgtttattactgt gcgagacatgaaggatataacagccgttactactactttggctac ARHEGYNSRYYYFGY IGHV5-51 IGHD6-25 IGHJ4 ggatacagctttgccaccttctgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggaatg atctttcctggtgactccgatacc agatacagcccgtccttccaaggccaggtcaccttctcagccgacaagtccatcaacaccgcctacctgcagttgaacagcctgacggcctcagacaccgccgtttattactgt gcgagacatgaaggatataacagccgttactactactttggctac IGA1 15 213 7 8 4 8 3 7 2 1 8 4 JY8QFUQ01B0YFD 2293 productive (see comment) Homsap IGHV4-39*02 F 951 92.27 203/220 nt 91.82 202/220 nt Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD1-26*01 F 3 10 7 13 10.7.13 [1.16.38.11] CARRYSWNYERFDPW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 64 in the user submitted sequence), (cause frameshift) tctctggtgactccatcagtactaataattactactgggcctggatccgccagcccccagggaggggctggagtggattgggaatatctattatagcgggaccacctactacaatccgtccctcaagagtcgagtcaccatgtccgtagacacgtccaagaaccacttctccctgaggttgagttctgtgaccgccgcagacacggctctctattactgcgcgcggcgatatagttggaactacgaaaggttcgacccctggggccagggaaccctggtcatcgtctcctcagcctccaccaagggcccatngtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 88 [290, 291] g83>a,G28>D(- - -)|c93>t|g101>c,S34>T(+ - +)|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|g119>c,G40>A(- + -)|g164>a,S55>N(- - -)|t177>c|g191>c,S64>T(+ - +)|c204>t|a234>g,I78>M(+ + -)|a269>g,K90>R(+ + +)|c271>t|c276>t|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t312>c| g83>a,G28>D(- - -)|c93>t|g101>c,S34>T(+ - +)|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)| g119>c,G40>A(- + -)|g164>a,S55>N(- - -)| t177>c|g191>c,S64>T(+ - +)| c204>t|a234>g,I78>M(+ + -)|a269>g,K90>R(+ + +)|c271>t|c276>t|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t312>c| a316>c|a318>g|g319>c|a320>g| 315 (320) 223 (228) 206 (207) 17 (21) 6 (10) 11 2 (4) 4 4 2 0 (1) 0 0 0 5 (6) 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 25 5 1 4 0 3 1 0 0 0 0 0 1 0 0 0 51 50 48 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 30 21 19 2 1 1 0 0 0 1 0 0 0 0 1 0 0 0 117 114 106 8 4 4 2 0 3 1 0 0 0 0 2 0 0 0 3 (8) 3 3 (4) 0 (4) 0 (4) 0 0 (2) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatcagtactaataattactac tgggcctggatccgccagcccccagggaggggctggagtggattgggaat atctattatagcgggaccacc tactacaatccgtccctcaagagtcgagtcaccatgtccgtagacacgtccaagaaccacttctccctgaggttgagttctgtgaccgccgcagacacggctctctattactgc gcgcggcgatatagttggaactacgaaaggttcgacccc ARRYSWNYERFDP IGHV4-39 IGHD1-26 IGHJ5 ggtgactccatcagtactaataattactac tgggcctggatccgccagcccccagggaggggctggagtggattgggaat atctattatagcgggaccacc tactacaatccgtccctcaagagtcgagtcaccatgtccgtagacacgtccaagaaccacttctccctgaggttgagttctgtgaccgccgcagacacggctctctattactgc gcgcggcgatatagttggaactacgaaaggttcgacccc IGG1 17 215 12 5 8 13 4 4 4 2 6 5 JY8QFUQ01B0Z32 18314 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 918 91.71 199/217 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-3*01 F 2 8 8 10 8.8.10 [1.17.38.11] CASDLGGQKSYW in-frame + ctcaggattcaccttcagtagatactggatgcactgggtccgccaagctccagggaaggggccggtgtgggtctcacgtactaatgaagatggcacccacataaattacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtatttgcaaatgaacagtctgagagccgaggacacggctgtctattactgtgcaagcgatttgggtggtcagaaaagttactggggccagggagtcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [278] t78>a|c108>a,S36>R(- - -)|t149>c,L50>P(- - -)|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>c|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|c194>t,T65>I(- - -)|g197>a,S66>N(- - -)|c198>t,S66>N(- - -)|c257>a,T86>K(- - -)|c265>t|g303>c|a318>c,R106>S(- - -)| t78>a| c108>a,S36>R(- - -)| t149>c,L50>P(- - -)| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>c|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|c194>t,T65>I(- - -)| g197>a,S66>N(- - -)|c198>t,S66>N(- - -)|c257>a,T86>K(- - -)|c265>t|g303>c| a318>c,R106>S(- - -)| 320 225 206 19 4 15 1 3 3 3 2 2 0 2 3 0 0 0 78 4 3 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 30 24 14 10 1 9 1 2 1 2 1 0 0 1 2 0 0 0 117 114 109 5 2 3 0 1 2 0 0 1 0 0 1 0 0 0 8 8 7 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctca ggattcaccttcagtagatactgg atgcactgggtccgccaagctccagggaaggggccggtgtgggtctcacgt actaatgaagatggcacccacata aattacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtatttgcaaatgaacagtctgagagccgaggacacggctgtctattactgt gcaagcgatttgggtggtcagaaaagttac ASDLGGQKSY IGHV3-74 IGHD3-3 IGHJ4 ggattcaccttcagtagatactgg atgcactgggtccgccaagctccagggaaggggccggtgtgggtctcacgt actaatgaagatggcacccacata aattacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtatttgcaaatgaacagtctgagagccgaggacacggctgtctattactgt gcaagcgatttgggtggtcagaaaagttac IGA2 17 213 10 7 6 11 4 6 2 1 4 10 JY8QFUQ01B0ZM2 17535 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 954 93.55 203/217 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD5-12*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARGPRSRGYGMDVW in-frame - ctctggattcaccttcaacaactatgccatgagttgggtccgccaggctccagggaaggggctggaatgggtctcaactattactagtggtggtggtagtacattgtacgcagactccgtgaagggccggttcaccatctccagagacaatttcaaggacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgagagggcctcgatcgcgcggctacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [288] t90>c|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|c120>t|g153>a|g163>a,A55>T(- - -)|g170>c,S57>T(+ - +)|g172>a,G58>S(+ + -)|a175>g,S59>G(+ + -)|c192>t|a197>t,Y66>L(- - -)|c198>g,Y66>L(- - -)|c248>t,S83>F(- - -)|a253>g,N85>D(+ + -)|a317>g,K106>R(+ + +)| t90>c|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)| c120>t|g153>a|g163>a,A55>T(- - -)| g170>c,S57>T(+ - +)|g172>a,G58>S(+ + -)|a175>g,S59>G(+ + -)|c192>t| a197>t,Y66>L(- - -)|c198>g,Y66>L(- - -)|c248>t,S83>F(- - -)|a253>g,N85>D(+ + -)| a317>g,K106>R(+ + +)|a320>g| 319 (320) 224 (225) 209 15 (16) 4 (5) 11 3 (4) 5 3 1 0 0 1 0 1 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 2 0 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 2 1 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 1 1 1 0 0 0 0 0 1 0 0 0 117 114 110 4 0 4 1 0 1 0 0 0 1 0 0 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 1 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcaacaactatgcc atgagttgggtccgccaggctccagggaaggggctggaatgggtctcaact attactagtggtggtggtagtaca ttgtacgcagactccgtgaagggccggttcaccatctccagagacaatttcaaggacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagagggcctcgatcgcgcggctacggtatggacgtc ARGPRSRGYGMDV IGHV3-23 IGHD5-12 IGHJ6 ggattcaccttcaacaactatgcc atgagttgggtccgccaggctccagggaaggggctggaatgggtctcaact attactagtggtggtggtagtaca ttgtacgcagactccgtgaagggccggttcaccatctccagagacaatttcaaggacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagagggcctcgatcgcgcggctacggtatggacgtc IGG2 14 213 11 3 8 10 3 4 2 2 5 5 JY8QFUQ01B11DU 17712 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 1013 96.33 210/218 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-21*02 F 3 8 8 20 8.8.20 [1.17.38.11] CARLPYIVVLTNIISPTYFDFW in-frame - tttctggatacagttttaccagttactggatcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggtatcatctatcctgctgactctgataccagatacagcccgtccttccaaggccaggtcagcatctcagccgacaagtccatcgacaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatgtattactgtgcgcggctgccatatattgtggtgctgactaatataatctccccaacctactttgacttctggggccagggaacccgggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [310] g74>t|c87>t|c108>t|g162>t|g176>c,G59>A(- + -)|c230>g,T77>S(+ - +)|a253>g,S85>D(- - -)|g254>a,S85>D(- - -)| g74>t| c87>t|c108>t| g162>t| g176>c,G59>A(- + -)| c230>g,T77>S(+ - +)|a253>g,S85>D(- - -)|g254>a,S85>D(- - -)| a316>c|a318>g|a320>t| 315 (320) 221 (226) 213 (215) 8 (11) 4 (7) 4 1 (2) 1 2 0 0 (1) 0 0 (1) 0 1 1 2 0 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 36 24 22 2 2 0 0 0 2 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 30 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 117 114 111 3 0 3 1 1 0 0 0 0 0 0 0 1 0 0 3 (8) 3 3 (5) 0 (3) 0 (3) 0 0 (1) 0 0 0 0 (1) 0 0 (1) 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| tttct ggatacagttttaccagttactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggtatc atctatcctgctgactctgatacc agatacagcccgtccttccaaggccaggtcagcatctcagccgacaagtccatcgacaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatgtattactgt gcgcggctgccatatattgtggtgctgactaatataatctccccaacctactttgacttc ARLPYIVVLTNIISPTYFDF IGHV5-51 IGHD2-21 IGHJ4 ggatacagttttaccagttactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggtatc atctatcctgctgactctgatacc agatacagcccgtccttccaaggccaggtcagcatctcagccgacaagtccatcgacaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatgtattactgt gcgcggctgccatatattgtggtgctgactaatataatctccccaacctactttgacttc IGG1 7 213 4 3 3 6 1 1 1 2 3 1 JY8QFUQ01B14S8 19885 productive (see comment) Homsap IGHV3-33*05 F (see comment) 742 82.87 179/216 nt 82.41 178/216 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-12*01 F 3 8 8 13 8.8.13 [1.16.38.11] CVKGRGYNDYGLDYW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (82,87% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 58 in the user submitted sequence), (cause frameshift) gtctggattctccttcagcaattatgccatccactgggtccgccaggctccaggcaagggctggagtgggtggcgaccatttcatatgatattaataaaagatattatgcagagtccgtgaggggccgattcaccctctccagagacaattccaagaacactctcgatctgctcatggatacccttcggttcgacgacacggctgtctattattgtgttaaaggacgtggatacaatgactacggcctcgactactggggcccgggaaccctgctcaccgtctcctcagcctccaccaagggcccatcggtcttcccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [288, 287] a85>t,T29>S(+ - +)|t105>c|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g113>c,G38>A(- + -)|g117>c,M39>I(+ + -)|a162>g|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)|t165>c,V55>T(- - -)|a168>t|g184>a,G62>I(- - -)|g185>t,G62>I(- - -)|a186>t,G62>I(- - -)|g188>a,S63>N(- - -)|t192>a,N64>K(+ - -)|a194>g,K65>R(+ + +)|c198>t|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|a232>c,I78>L(+ + +)|g258>t|g261>c|t262>g,Y88>D(- - -)|a269>t,Q90>L(- - -)|a270>c,Q90>L(- - -)|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|g278>c,S93>T(+ - +)|g282>t|a283>c|a285>g|g286>t,A96>F(+ - -)|c287>t,A96>F(+ - -)|g291>c,E97>D(+ - +)|g303>c|c309>t| a85>t,T29>S(+ - +)|t105>c|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g113>c,G38>A(- + -)| g117>c,M39>I(+ + -)|a162>g|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)|t165>c,V55>T(- - -)| a168>t|g184>a,G62>I(- - -)|g185>t,G62>I(- - -)|a186>t,G62>I(- - -)|g188>a,S63>N(- - -)|t192>a,N64>K(+ - -)|a194>g,K65>R(+ + +)| c198>t|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|a232>c,I78>L(+ + +)|g258>t|g261>c|t262>g,Y88>D(- - -)|a269>t,Q90>L(- - -)|a270>c,Q90>L(- - -)|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|g278>c,S93>T(+ - +)|g282>t|a283>c|a285>g|g286>t,A96>F(+ - -)|c287>t,A96>F(+ - -)|g291>c,E97>D(+ - +)|g303>c|c309>t| c314>t,A105>V(+ - +)|g315>t,A105>V(+ - +)|g317>a,R106>K(+ + +)|a320>g| 319 (320) 223 (224) 183 40 (41) 11 (12) 29 5 (6) 5 6 3 3 0 4 1 6 1 5 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 1 1 0 0 1 0 1 0 0 0 51 50 45 5 1 4 1 1 0 2 0 0 0 0 1 0 0 0 30 24 17 7 1 6 1 2 0 0 0 0 2 1 0 0 1 0 117 114 94 20 8 12 3 0 4 0 3 0 1 0 4 1 3 1 7 (8) 7 4 3 (4) 0 (1) 3 0 (1) 1 1 0 0 0 0 0 0 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattctccttcagcaattatgcc atccactgggtccgccaggctccaggcaagggctggagtgggtggcgacc atttcatatgatattaataaaaga tattatgcagagtccgtgaggggccgattcaccctctccagagacaattccaagaacactctcgatctgctcatggatacccttcggttcgacgacacggctgtctattattgt gttaaaggacgtggatacaatgactacggcctcgactac VKGRGYNDYGLDY IGHV3-33 IGHD5-12 IGHJ4 ggattctccttcagcaattatgcc atccactgggtccgccaggctccaggcaagggctggagtgggtggcgacc atttcatatgatattaataaaaga tattatgcagagtccgtgaggggccgattcaccctctccagagacaattccaagaacactctcgatctgctcatggatacccttcggttcgacgacacggctgtctattattgt gttaaaggacgtggatacaatgactacggcctcgactac IGG1 37 212 17 20 9 20 8 17 9 2 16 10 JY8QFUQ01B1B92 20699 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 810 86.18 187/217 nt 85.71 186/217 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD6-19*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKDTITMGGDVAPLFDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 41 of V-REGION: 'G' (1 nucleotides at position 36 to 36 in the user submitted sequence), (cause frameshift); ctctggattcacctttagtaactatgacatgggctGgggtccgccaggctccagggaagggactggagtgggtctcaagtattacgactggtggtgagagaacttactatgcagactccgtgaagggccgattcaccatcgccagagacaactcccagagcacgatgtttctgcaaatgaacagcctgagggccgacgacgcggccatctactactgtgcgaaagacaccataacaatgggcggtgacgtcgctccactgtttgactactggggtcagggaaccctggtcaccgtctcctcgtcatccccgaccagcccccaaggtcttcccgctgagcctctgcagcaccccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccca NA IGA1 79 89 [301, 302, 300] c105>t|g107>a,S36>N(- - -)|c113>a,A38>D(- - -)|a118>g,S40>G(+ + -)|g147>a|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|g170>c,S57>T(+ - +)|t171>g,S57>T(+ - +)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|a175>g,S59>G(+ + -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)|a195>t|c201>t|g225>a|t235>g,S79>A(- + -)|t246>c|a250>c,K84>Q(+ - -)|a254>g,N85>S(- - -)|c259>a,L87>M(+ + -)|a263>t,Y88>F(- + -)|a285>g|g291>c,E97>D(+ - +)|a295>g,T99>A(- - -)|g301>a,V101>I(+ - +)|a303>c,V101>I(+ - +)|t306>c| c105>t|g107>a,S36>N(- - -)|c113>a,A38>D(- - -)| a118>g,S40>G(+ + -)|g147>a|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| g170>c,S57>T(+ - +)|t171>g,S57>T(+ - +)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|a175>g,S59>G(+ + -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)|a195>t| c201>t|g225>a|t235>g,S79>A(- + -)|t246>c|a250>c,K84>Q(+ - -)|a254>g,N85>S(- - -)|c259>a,L87>M(+ + -)|a263>t,Y88>F(- + -)|a285>g|g291>c,E97>D(+ - +)|a295>g,T99>A(- - -)|g301>a,V101>I(+ - +)|a303>c,V101>I(+ - +)|t306>c| 320 225 195 30 8 22 5 7 2 2 2 3 2 0 3 1 0 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 0 0 1 0 0 0 0 0 0 51 51 47 4 1 3 1 2 0 0 0 0 0 0 0 1 0 0 30 24 15 9 1 8 1 2 0 0 0 1 1 0 2 0 0 2 117 114 100 14 5 9 3 2 1 2 2 1 1 0 1 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtaactatgac atgggctgggtccgccaggctccagggaagggactggagtgggtctcaagt attacgactggtggtgagagaact tactatgcagactccgtgaagggccgattcaccatcgccagagacaactcccagagcacgatgtttctgcaaatgaacagcctgagggccgacgacgcggccatctactactgt gcgaaagacaccataacaatgggcggtgacgtcgctccactgtttgactac AKDTITMGGDVAPLFDY IGHV3-23 IGHD6-19 IGHJ4 ggattcacctttagtaactatgac atgggctgggtccgccaggctccagggaagggactggagtgggtctcaagt attacgactggtggtgagagaact tactatgcagactccgtgaagggccgattcaccatcgccagagacaactcccagagcacgatgtttctgcaaatgaacagcctgagggccgacgacgcggccatctactactgt gcgaaagacaccataacaatgggcggtgacgtcgctccactgtttgactac IGA1 30 213 16 14 9 16 7 14 6 2 12 10 JY8QFUQ01B1CH3 12814 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 864 88.48 192/217 nt 87.56 190/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 15 8.8.15 [1.17.38.11] CAKDASRYYYNSYCDNW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 12 to 12 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 102 of V-REGION: 'C' (1 nucleotides at position 212 to 212 in the user submitted sequence), (cause frameshift); ctctggattcaGcctttaccaactacgccatgagctgggttcgccaggttccagggaaggggctggagtgggtctcacttattagtgttcgtggcgatgacaccttctatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtatctgcaaatggacatcctgaaacccgaggacacggccgtttaCtttttgcgcgaaagatgcgtctcgatattattataacagttattgtgacaattggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [296] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|t111>c|c126>t|c134>t,A45>V(+ - +)|g163>c,A55>L(+ - +)|c164>t,A55>L(+ - +)|g173>t,G58>V(- - -)|a175>c,S59>R(- - -)|t186>c|g188>a,G63>D(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a195>c|a197>t,Y66>F(- + -)|c201>t|t246>c|a274>g,N92>D(+ + -)|g278>t,S93>I(- - -)|g284>a,R95>K(+ + +)|g286>c,A96>P(- - -)|a303>t|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)|t312>c| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|t111>c| c126>t|c134>t,A45>V(+ - +)|g163>c,A55>L(+ - +)|c164>t,A55>L(+ - +)| g173>t,G58>V(- - -)|a175>c,S59>R(- - -)|t186>c|g188>a,G63>D(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a195>c| a197>t,Y66>F(- + -)|c201>t|t246>c|a274>g,N92>D(+ + -)|g278>t,S93>I(- - -)|g284>a,R95>K(+ + +)|g286>c,A96>P(- - -)|a303>t|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)|t312>c| 320 225 200 25 8 17 2 4 5 4 2 0 3 0 3 0 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 0 1 0 0 0 0 1 0 0 0 51 51 47 4 1 3 0 0 3 0 0 0 0 0 1 0 0 0 30 24 17 7 2 5 1 2 0 1 2 0 0 0 0 0 1 0 117 114 103 11 4 7 1 1 2 2 0 0 3 0 1 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaccaactacgcc atgagctgggttcgccaggttccagggaaggggctggagtgggtctcactt attagtgttcgtggcgatgacacc ttctatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtatctgcaaatggacatcctgaaacccgaggacacggccgtttatttttgc gcgaaagatgcgtctcgatattattataacagttattgtgacaat AKDASRYYYNSYCDN IGHV3-23 IGHD3-22 IGHJ4 ggattcacctttaccaactacgcc atgagctgggttcgccaggttccagggaaggggctggagtgggtctcactt attagtgttcgtggcgatgacacc ttctatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtatctgcaaatggacatcctgaaacccgaggacacggccgtttatttttgc gcgaaagatgcgtctcgatattattataacagttattgtgacaat IGG2 25 213 15 10 9 14 6 11 5 3 10 7 JY8QFUQ01B1HDC 14744 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*02 F or Homsap IGHV4-39*05 F (see comment) 794 84.62 187/221 nt 83.71 185/221 nt Homsap IGHJ4*02 F (see comment) 204 91.67 44/48 nt Homsap IGHD3-3*01 F 3 10 7 16 10.7.16 [1.17.38.11] CARHRSFGVIIVNYFESW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (84,62% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 39 of V-REGION: 'G' (1 nucleotides at position 38 to 38 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 45 of V-REGION: 'T' (1 nucleotides at position 55 to 55 in the user submitted sequence), (cause frameshift); tttccggtgactccatcaccagtactaattattactgGgggctgggtccgccagTcccccagggaagggtctggagtggattggaagtgtctattatcgggggacccagtacctcaacccgtctctccacaatcgagtcaccatatccattggcacgtccaagacccaattctccatgagactgacctctgtgaccgccgcagacacggctgtatatttctgtgcgagacatcggtcttttggagtgataattgttaactactttgagtcctggggacaggggaccctggtcaccgtctcctcagcttccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccggaaccggtgacggtgtcgtggaactc NA IGG3 86 94 [303] c75>t|t78>c|g83>a,G28>D(- - -)|g92>c,S31>T(+ - +)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c111>t|a124>g,I42>V(+ - +)|g147>t|g162>a|a166>g,I56>V(+ - +)|a175>c,S59>R(- - -)|t177>g,S59>R(- - -)|g191>c,S64>T(+ - +)|a193>c,T65>Q(- - -)|c194>a,T65>Q(- - -)|c195>g,T65>Q(- - -)|t199>c,Y67>L(- - -)|a200>t,Y67>L(- - -)|c210>t|a214>c,K72>H(- - +)|g216>c,K72>H(- - +)|g221>a,S74>N(- - -)|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|a242>g,D81>G(- - -)|a254>c,N85>T(- + -)|g258>a|c265>a,L89>M(+ + -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|g275>c,S92>T(+ - +)|g303>a|a308>t,Y103>F(- + -)| c75>t|t78>c| g83>a,G28>D(- - -)|g92>c,S31>T(+ - +)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c111>t| a124>g,I42>V(+ - +)|g147>t|g162>a| a166>g,I56>V(+ - +)|a175>c,S59>R(- - -)|t177>g,S59>R(- - -)|g191>c,S64>T(+ - +)|a193>c,T65>Q(- - -)|c194>a,T65>Q(- - -)|c195>g,T65>Q(- - -)| t199>c,Y67>L(- - -)|a200>t,Y67>L(- - -)|c210>t|a214>c,K72>H(- - +)|g216>c,K72>H(- - +)|g221>a,S74>N(- - -)|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|a242>g,D81>G(- - -)|a254>c,N85>T(- + -)|g258>a|c265>a,L89>M(+ + -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|g275>c,S92>T(+ - +)|g303>a|a308>t,Y103>F(- + -)| 320 229 195 34 8 26 4 8 3 2 4 2 3 0 5 1 1 1 78 5 3 2 2 0 0 0 1 1 0 0 0 0 0 0 0 0 36 30 25 5 1 4 0 2 1 0 0 0 0 0 2 0 0 0 51 51 48 3 2 1 1 1 0 0 0 0 0 0 0 0 1 0 30 21 14 7 0 7 1 0 0 0 2 1 0 0 1 1 0 1 117 114 97 17 3 14 2 5 1 1 2 1 3 0 2 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tttcc ggtgactccatcaccagtactaattattac tggggctgggtccgccagcccccagggaagggtctggagtggattggaagt gtctattatcgggggacccag tacctcaacccgtctctccacaatcgagtcaccatatccattggcacgtccaagacccaattctccatgagactgacctctgtgaccgccgcagacacggctgtatatttctgt gcgagacatcggtcttttggagtgataattgttaactactttgagtcc ARHRSFGVIIVNYFES IGHV4-39 IGHD3-3 IGHJ4 ggtgactccatcaccagtactaattattac tggggctgggtccgccagcccccagggaagggtctggagtggattggaagt gtctattatcgggggacccag tacctcaacccgtctctccacaatcgagtcaccatatccattggcacgtccaagacccaattctccatgagactgacctctgtgaccgccgcagacacggctgtatatttctgt gcgagacatcggtcttttggagtgataattgttaactactttgagtcc IGG3 32 216 15 17 10 19 5 13 5 1 15 11 JY8QFUQ01B1HSY 16585 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 809 86.51 186/215 nt 85.12 183/215 nt Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-16*02 F 2 7 7 9 7.7.9 [1.16.38.11] CTGLTYPDDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 38 of V-REGION: 1 nucleotides (from position 29 in the user submitted sequence), (cause frameshift)in FR2-IMGT, from codon 49 of V-REGION: 1 nucleotides (from position 59 in the user submitted sequence), (cause frameshift)in CDR2-IMGT, from codon 56 of V-REGION: 1 nucleotides (from position 81 in the user submitted sequence), (cause frameshift) cgtctggatttaccttcagtaagttctgatgcattgggtccgccaagctccagggaagggctgacttgggtctcacgtataatcctgatgggactatcacgaactacacggactccgtgaggggccgattcatcacttccagagacaacgccaagaacacagtatatctgcagatgaacagtctgcgagtcgaggacacaggtgtatattactgtacaggactgacttatccagacgactactggggccagggatgtttggccaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [273] c75>g|c84>t|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a110>t,Y37>F(- + -)|c120>t|g151>a,V51>T(- - -)|t152>c,V51>T(- - -)|g153>t,V51>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g188>c,S63>T(+ - +)|g191>t,S64>I(- - -)|a195>g|g197>a,S66>N(- - -)|g202>a,A68>T(- - -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|t233>c,I78>T(- - -)|c234>t,I78>T(- - -)|g258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a270>g|a283>c|c287>t,A96>V(+ - +)|g297>a|c299>g,A100>G(- + -)|g303>a|g313>a,A105>T(- - -)|a316>g,R106>G(- - -)| c75>g| c84>t|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a110>t,Y37>F(- + -)| c120>t|g151>a,V51>T(- - -)|t152>c,V51>T(- - -)|g153>t,V51>T(- - -)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g188>c,S63>T(+ - +)|g191>t,S64>I(- - -)|a195>g| g197>a,S66>N(- - -)|g202>a,A68>T(- - -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|t233>c,I78>T(- - -)|c234>t,I78>T(- - -)|g258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a270>g|a283>c|c287>t,A96>V(+ - +)|g297>a|c299>g,A100>G(- + -)|g303>a| g313>a,A105>T(- - -)|a316>g,R106>G(- - -)|g319>c|a320>t| 318 (320) 221 (223) 190 31 (33) 9 (11) 22 4 9 5 2 2 0 1 (2) 0 2 (3) 4 2 0 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 23 19 4 1 3 0 1 1 0 0 0 1 0 0 1 0 0 51 50 46 4 1 3 0 1 1 1 0 0 0 0 0 0 1 0 30 23 18 5 1 4 1 0 0 0 1 0 0 0 2 0 1 0 117 114 99 15 5 10 2 6 3 1 1 0 0 0 0 2 0 0 6 (8) 6 4 2 (4) 0 (2) 2 1 1 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cgtct ggatttaccttcagtaagttctg atgcattgggtccgccaagctccagggaagggctgacttgggtctcacgt ataatcctgatgggactatcacg aactacacggactccgtgaggggccgattcatcacttccagagacaacgccaagaacacagtatatctgcagatgaacagtctgcgagtcgaggacacaggtgtatattactgt acaggactgacttatccagacgactac TGLTYPDDY IGHV3-74 IGHD3-16 IGHJ4 ggatttaccttcagtaagttctg atgcattgggtccgccaagctccagggaagggctgacttgggtctcacgt ataatcctgatgggactatcacg aactacacggactccgtgaggggccgattcatcacttccagagacaacgccaagaacacagtatatctgcagatgaacagtctgcgagtcgaggacacaggtgtatattactgt acaggactgacttatccagacgactac IGA1 28 210 18 10 13 20 5 8 6 2 13 7 JY8QFUQ01B1L81 19736 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 890 90.7 195/215 nt 89.3 192/215 nt Homsap IGHJ5*02 F 228 94.12 48/51 nt Homsap IGHD4-17*01 F 2 8 7 15 8.7.15 [1.17.38.11] CAKNNNDFGDYDWFDPW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 48 to 48 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 63 of V-REGION: 'G' (1 nucleotides at position 96 to 96 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 69 of V-REGION: 'C' (1 nucleotides at position 114 to 114 in the user submitted sequence), (cause frameshift); tctctggtgtctccatcagtagttcctactggagttggatccggcagCcccccagggaagggcctggagtggattggttatgtctattatactggGgggcaccgactccaaccCcctccctcaagagtcgtgtcaccctgtcaatggacacgtccaagaaccagttctccttggaactgagctctgtgaccgctgcggacacggccgtctattactgtgcgaaaaacaacaatgacttcggtgactacgactggttcgacccctggggccagggaatgctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagccctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 86 95 [295, 294] g83>t,G28>V(- - -)|a110>c,Y37>S(+ - -)|c120>t|a147>c|g162>t|a166>g,I56>V(+ - +)|c174>t|g176>c,S59>T(+ - +)|a190>g,S64>G(+ + -)|a196>g,N66>D(+ + -)|a200>c,Y67>S(+ - -)|a225>t|a232>c,I78>L(+ + +)|a234>g,I78>L(+ + +)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|c265>t|a268>g,K90>E(+ - -)|g270>a,K90>E(+ - -)|g303>c| g83>t,G28>V(- - -)|a110>c,Y37>S(+ - -)| c120>t|a147>c|g162>t| a166>g,I56>V(+ - +)|c174>t|g176>c,S59>T(+ - +)|a190>g,S64>G(+ + -)| a196>g,N66>D(+ + -)|a200>c,Y67>S(+ - -)|a225>t|a232>c,I78>L(+ + +)|a234>g,I78>L(+ + +)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|c265>t|a268>g,K90>E(+ - -)|g270>a,K90>E(+ - -)|g303>c| g317>a,R106>K(+ + +)|g319>a| 316 (320) 219 (223) 199 (201) 20 (22) 7 (8) 13 (14) 6 2 (4) 3 0 4 0 1 0 2 0 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 1 0 0 0 0 0 1 0 51 51 48 3 3 0 0 0 1 0 1 0 0 0 0 0 1 0 30 21 17 4 1 3 2 0 1 0 0 0 0 0 1 0 0 0 117 114 103 11 3 8 4 2 1 0 2 0 1 0 1 0 0 0 4 (8) 4 4 (6) 0 (2) 0 (1) 0 (1) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgtctccatcagtagttcctac tggagttggatccggcagcccccagggaagggcctggagtggattggttat gtctattatactgggggcacc gactccaacccctccctcaagagtcgtgtcaccctgtcaatggacacgtccaagaaccagttctccttggaactgagctctgtgaccgctgcggacacggccgtctattactgt gcgaaaaacaacaatgacttcggtgactacgactggttcgacccc AKNNNDFGDYDWFDP IGHV4-59 IGHD4-17 IGHJ5 ggtgtctccatcagtagttcctac tggagttggatccggcagcccccagggaagggcctggagtggattggttat gtctattatactgggggcacc gactccaacccctccctcaagagtcgtgtcaccctgtcaatggacacgtccaagaaccagttctccttggaactgagctctgtgaccgctgcggacacggccgtctattactgt gcgaaaaacaacaatgacttcggtgactacgactggttcgacccc IGA1 20 210 11 9 5 9 6 11 6 1 8 5 JY8QFUQ01B1N7G 11036 productive Homsap IGHV3-49*03 F, or Homsap IGHV3-49*05 F 867 87.44 195/223 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-10*02 F 2 8 10 12 8.10.12 [1.17.38.11] CSYFRHEPYHFDYW in-frame + cgctggattcgcctctggtgattatgctatgagttggttccgccaggctccgggaaaggggctggagtgggtggggttcatcagaagcagagcttacactgggacaccagaatacgccgcgtctgtgaaaggcagattcaccatctccagagatgcttccaaaagtattggctatctgcaaatgaacaatctgaggatcgaggacacagccgtctattattgctcttattttcggcatgagccatatcactttgactattggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [290] t75>c|t76>g,S26>A(- + -)|a85>g,T29>A(- - -)|t89>c,F30>S(- - -)|c120>t|a138>g|g141>a|a159>g|t162>g|t168>c|a176>g,K59>R(+ + +)|t183>c|g184>a,G62>T(+ - -)|g185>c,G62>T(+ - -)|a193>c,T65>P(+ + -)|a237>c|a245>c,D82>A(- - -)|c255>t|c258>t|c260>g,A87>G(- + -)|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|a284>g,K95>R(+ + +)|a285>g,K95>R(+ + +)|c287>t,T96>I(- - -)|g303>c|c309>t| t75>c|t76>g,S26>A(- + -)| a85>g,T29>A(- - -)|t89>c,F30>S(- - -)| c120>t|a138>g|g141>a|a159>g|t162>g| t168>c|a176>g,K59>R(+ + +)|t183>c|g184>a,G62>T(+ - -)|g185>c,G62>T(+ - -)|a193>c,T65>P(+ + -)| a237>c|a245>c,D82>A(- - -)|c255>t|c258>t|c260>g,A87>G(- + -)|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|a284>g,K95>R(+ + +)|a285>g,K95>R(+ + +)|c287>t,T96>I(- - -)|g303>c|c309>t|t312>c| a313>t,T105>S(+ - +)|a316>t,R106>Y(- - -)|g317>a,R106>Y(- - -)|a318>t,R106>Y(- - -)|g319>t|a320>t| 311 (320) 222 (231) 195 (197) 27 (34) 13 (16) 14 (18) 6 3 (4) 6 4 (5) 3 0 0 (4) 0 2 1 0 (1) 2 78 4 2 2 1 1 0 0 0 1 0 0 0 0 0 0 0 1 36 24 22 2 0 2 1 0 0 1 0 0 0 0 0 0 0 0 51 51 46 5 5 0 2 1 1 0 0 0 0 0 0 0 0 1 30 30 24 6 2 4 1 1 0 2 1 0 0 0 1 0 0 0 116 (117) 114 101 12 5 7 2 1 5 0 2 0 0 0 1 1 0 0 0 (8) 0 0 (2) 0 (6) 0 (2) 0 (4) 0 0 (1) 0 0 (0) 0 0 0 (4) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,158-159(FR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,181-182(CDR2)|aa,192-193(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|ta,69-70(FR1)|tt,75-76(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,124-125(FR2)|ta,158-159(FR2)|tt,162-163(FR2)|tt,167-168(CDR2)|tt,180-181(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agct,72-75(FR1)|agct,118-121(FR2)|ggtt,122-125(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggta,156-159(FR2)|ggtt,160-163(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|ggca,220-223(FR3)|agca,253-256(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,72-75(FR1)|tgct,111-114(CDR1)|agct,118-121(FR2)|agct,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| cgct ggattcgcctctggtgattatgct atgagttggttccgccaggctccgggaaaggggctggagtgggtggggttc atcagaagcagagcttacactgggacacca gaatacgccgcgtctgtgaaaggcagattcaccatctccagagatgcttccaaaagtattggctatctgcaaatgaacaatctgaggatcgaggacacagccgtctattattgc tcttattttcggcatgagccatatcactttgactat SYFRHEPYHFDY IGHV3-49 IGHD3-10 IGHJ4 ggattcgcctctggtgattatgct atgagttggttccgccaggctccgggaaaggggctggagtgggtggggttc atcagaagcagagcttacactgggacacca gaatacgccgcgtctgtgaaaggcagattcaccatctccagagatgcttccaaaagtattggctatctgcaaatgaacaatctgaggatcgaggacacagccgtctattattgc tcttattttcggcatgagccatatcactttgactat IGA1 25 219 18 7 9 12 9 13 10 2 7 6 JY8QFUQ01B1RUY 16053 productive Homsap IGHV1-69*01 F, or Homsap IGHV1-69*11 F or Homsap IGHV1-69*12 F or Homsap IGHV1-69*13 (F) or Homsap IGHV1-69D*01 F 795 85.51 183/214 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-22*01 F 2 8 8 12 8.8.12 [0.17.38.11] CVRVRDYEIGGDFW in-frame + tggaggcgccttcagcacctttcatatcacctgggtgcgacaggcccctggacaagggcttgtctggctggggaatatcattcctatttttggaacagtcgactacgcacgggggttccagggcagagtcacgattaccgcggacgaatccaccaacacagcctacatggagttgagcagcctgacatctgaggactcagccgtctatttctgcgtgagagttcgtgactatgaaattggtggtgacttctggggccagggcaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaac NA IGG1 100 100 [282] a85>g,T29>A(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|g112>c,A38>H(- - -)|c113>a,A38>H(- - -)|g119>c,S40>T(+ - +)|a152>t,E51>V(- + -)|g153>c,E51>V(- + -)|a157>c,M53>L(+ + -)|a162>g|g163>a,G55>N(- - -)|g164>a,G55>N(- - -)|g165>t,G55>N(- - -)|c171>t|c177>t|t189>a|c194>t,A65>V(+ - +)|a195>c,A65>V(+ - +)|a196>g,N66>D(+ + -)|a206>g,Q69>R(+ - -)|a208>g,K70>G(- - -)|a209>g,K70>G(- - -)|g252>c|g254>a,S85>N(- - -)|c271>t|g284>c,R95>T(- - -)|a295>t,T99>S(+ - +)|g297>a,T99>S(+ - +)|g303>c|a308>t,Y103>F(- + -)|t312>c|c314>t,A105>V(+ - +)| a85>g,T29>A(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|g112>c,A38>H(- - -)|c113>a,A38>H(- - -)| g119>c,S40>T(+ - +)|a152>t,E51>V(- + -)|g153>c,E51>V(- + -)|a157>c,M53>L(+ + -)|a162>g|g163>a,G55>N(- - -)|g164>a,G55>N(- - -)|g165>t,G55>N(- - -)| c171>t|c177>t|t189>a|c194>t,A65>V(+ - +)|a195>c,A65>V(+ - +)| a196>g,N66>D(+ + -)|a206>g,Q69>R(+ - -)|a208>g,K70>G(- - -)|a209>g,K70>G(- - -)|g252>c|g254>a,S85>N(- - -)|c271>t|g284>c,R95>T(- - -)|a295>t,T99>S(+ - +)|g297>a,T99>S(+ - +)|g303>c|a308>t,Y103>F(- + -)|t312>c| c314>t,A105>V(+ - +)|a320>t| 319 (320) 221 (222) 189 32 (33) 8 (9) 24 6 4 5 1 2 1 4 (5) 1 7 0 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 0 0 0 0 1 1 0 2 0 0 0 51 51 43 8 1 7 1 2 0 0 1 0 1 0 2 0 1 0 30 24 19 5 3 2 0 0 3 0 1 0 0 1 0 0 0 0 117 114 101 13 4 9 4 2 1 1 0 0 2 0 3 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 0 0 (1) 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|aa,245-246(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|tt,184-185(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggca,82-85(CDR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agca,192-195(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggaggcgccttcagcacctttcat atcacctgggtgcgacaggcccctggacaagggcttgtctggctggggaat atcattcctatttttggaacagtc gactacgcacgggggttccagggcagagtcacgattaccgcggacgaatccaccaacacagcctacatggagttgagcagcctgacatctgaggactcagccgtctatttctgc gtgagagttcgtgactatgaaattggtggtgacttc VRVRDYEIGGDF IGHV1-69 IGHD3-22 IGHJ4 ggaggcgccttcagcacctttcat atcacctgggtgcgacaggcccctggacaagggcttgtctggctggggaat atcattcctatttttggaacagtc gactacgcacgggggttccagggcagagtcacgattaccgcggacgaatccaccaacacagcctacatggagttgagcagcctgacatctgaggactcagccgtctatttctgc gtgagagttcgtgactatgaaattggtggtgacttc IGG1 31 213 15 16 8 17 7 14 5 3 16 7 JY8QFUQ01B1TCN 13711 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 846 88.02 191/217 nt NA Homsap IGHJ6*02 F 202 80.65 50/62 nt Homsap IGHD2-21*02 F 3 8 7 16 8.7.16 [2.17.38.11] CAREFRAPSLARYGMDVW in-frame - gcctcgggggttcagcgtcagtaataacttcatgacctgggtccgccaggttccagggaaggggctggagtgggtctcagttatttatagcaatggtgaaacaatctacgcagactccgtgaagggccgattcactatgtccagagacaattccaagaacacactgtttcttcaaatgaacagcctgagaggcgaggacacggccgtgtaccactgtgcgagagaatttcgggcgccttctttggccagatacggtatggatgtctggggccacgggaccacggtcaccgtctcctcaatatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [296] a72>g|g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>R(- - -)|c77>g,S26>R(- - -)|t78>g,S26>R(- - -)|c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a113>t,Y38>F(- + -)|g119>c,S40>T(+ - +)|c134>t,A45>V(+ - +)|g175>a,G59>N(- - -)|g176>a,G59>N(- - -)|a190>g,S64>E(- - -)|g191>a,S64>E(- - -)|c192>a,S64>E(- - -)|t196>a,Y66>I(- - -)|a197>t,Y66>I(- - -)|c231>t|c234>g,I78>M(+ + -)|g258>a|a263>t,Y88>F(- + -)|c287>g,A96>G(- + -)|t306>c|t307>c,Y103>H(+ - -)| a72>g|g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>R(- - -)|c77>g,S26>R(- - -)|t78>g,S26>R(- - -)| c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a113>t,Y38>F(- + -)| g119>c,S40>T(+ - +)|c134>t,A45>V(+ - +)| g175>a,G59>N(- - -)|g176>a,G59>N(- - -)|a190>g,S64>E(- - -)|g191>a,S64>E(- - -)|c192>a,S64>E(- - -)| t196>a,Y66>I(- - -)|a197>t,Y66>I(- - -)|c231>t|c234>g,I78>M(+ + -)|g258>a|a263>t,Y88>F(- + -)|c287>g,A96>G(- + -)|t306>c|t307>c,Y103>H(+ - -)| 320 225 199 26 4 22 2 5 4 3 0 1 3 1 2 4 0 1 78 7 1 6 1 5 1 0 1 1 0 0 0 0 1 1 0 1 36 24 20 4 0 4 0 1 1 0 0 0 1 0 0 1 0 0 51 51 49 2 0 2 0 0 1 0 0 0 0 0 1 0 0 0 30 21 16 5 0 5 1 3 0 0 0 1 0 0 0 0 0 0 117 114 105 9 3 6 0 1 1 2 0 0 2 1 0 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctcgg gggttcagcgtcagtaataacttc atgacctgggtccgccaggttccagggaaggggctggagtgggtctcagtt atttatagcaatggtgaaaca atctacgcagactccgtgaagggccgattcactatgtccagagacaattccaagaacacactgtttcttcaaatgaacagcctgagaggcgaggacacggccgtgtaccactgt gcgagagaatttcgggcgccttctttggccagatacggtatggatgtc AREFRAPSLARYGMDV IGHV3-53 IGHD2-21 IGHJ6 gggttcagcgtcagtaataacttc atgacctgggtccgccaggttccagggaaggggctggagtgggtctcagtt atttatagcaatggtgaaaca atctacgcagactccgtgaagggccgattcactatgtccagagacaattccaagaacacactgtttcttcaaatgaacagcctgagaggcgaggacacggccgtgtaccactgt gcgagagaatttcgggcgccttctttggccagatacggtatggatgtc IGA1 20 210 11 9 8 13 3 7 3 0 8 9 JY8QFUQ01B1UHR 20665 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 946 92.69 203/219 nt 92.24 202/219 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-15*01 F 2 8 8 11 8.8.11 [2.17.38.11] CARSGSGSHLDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 40 of V-REGION: 'G' (1 nucleotides at position 34 to 34 in the user submitted sequence), (cause frameshift); gcctctggattcatcttcagtagctactggatgGcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtactaatacgggggggactagcacagcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacggtgtatctgcaaatgaacagcctgagagtcgacgacacggctgtgtattactgtgcgagaagtggtagtgggagccacttagattactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] c86>t,T29>I(- - -)|t167>c,I56>T(- - -)|g173>c,S58>T(+ - +)|t174>g,S58>T(+ - +)|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|c259>g,L87>V(+ - +)|t279>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|a315>g| c86>t,T29>I(- - -)| t167>c,I56>T(- - -)|g173>c,S58>T(+ - +)|t174>g,S58>T(+ - +)|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|c259>g,L87>V(+ - +)|t279>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| a315>g|g319>a|a320>g| 318 (320) 225 (227) 208 17 (19) 3 (5) 14 3 (4) 0 (1) 2 2 1 0 0 0 4 3 0 2 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 18 6 0 6 1 0 0 1 0 0 0 0 2 0 0 2 117 114 105 9 2 7 1 0 1 1 1 0 0 0 2 3 0 0 6 (8) 6 5 1 (3) 1 (3) 0 1 (2) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt actaatacgggggggactagcaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacggtgtatctgcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaagtggtagtgggagccacttagattac ARSGSGSHLDY IGHV3-74 IGHD2-15 IGHJ4 ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt actaatacgggggggactagcaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacggtgtatctgcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaagtggtagtgggagccacttagattac IGA1 16 213 6 10 2 9 4 7 2 0 7 7 JY8QFUQ01B1UYR 19317 productive Homsap IGHV3-21*04 F (see comment) 662 78.44 171/218 nt NA Homsap IGHJ4*02 F 105 68.75 33/48 nt Homsap IGHD5-12*01 F 3 8 8 13 8.8.13 [1.17.38.11] CVRLFTSDGVRGDYW in-frame + Low V-REGION identity (78,44% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release cctccggtttcatcttcagtcacttcagcatgaactgggtccgccaggctccaggaaagggcctggagtggatcgccgacatcagtagttcaagtgcatacatcacctatgcagattcagtcaggggccgattcgtcgtctccagggacgacgccaaggactccctgtacttgcaaatggacaacctgggagtcgacgatacggccacctattattgtgtgaggctcttcaccagcgatggagtgagaggcgactactggggccagggcgcccgggttctcgtctccgcagcttccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG3 100 100 [289] t78>c|a81>t|c86>t,T29>I(- - -)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a110>t,Y37>F(- + -)|t111>c,Y37>F(- + -)|g141>a|g147>c|g157>a,V53>I(+ - +)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)|t163>g,S55>D(- - -)|c164>a,S55>D(- - -)|t168>c|a175>t|g176>c|t177>a|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>a,S63>A(- + -)|a195>c|t196>a,Y66>T(+ - -)|a197>c,Y66>T(+ - -)|c201>t|c207>t|g213>c|a215>g,K72>R(+ + +)|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|a232>g,I78>V(+ - +)|a240>g|a244>g,N82>D(+ + -)|a253>g,N85>D(+ + -)|a258>c|t264>c|c265>t|a274>g,N92>D(+ + -)|g278>a,S93>N(- - -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c294>t|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>c,V101>T(- - -)|c309>t|c314>t,A105>V(+ - +)| t78>c| a81>t|c86>t,T29>I(- - -)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a110>t,Y37>F(- + -)|t111>c,Y37>F(- + -)| g141>a|g147>c|g157>a,V53>I(+ - +)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)|t163>g,S55>D(- - -)|c164>a,S55>D(- - -)| t168>c|a175>t|g176>c|t177>a|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>a,S63>A(- + -)|a195>c| t196>a,Y66>T(+ - -)|a197>c,Y66>T(+ - -)|c201>t|c207>t|g213>c|a215>g,K72>R(+ + +)|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|a232>g,I78>V(+ - +)|a240>g|a244>g,N82>D(+ + -)|a253>g,N85>D(+ + -)|a258>c|t264>c|c265>t|a274>g,N92>D(+ + -)|g278>a,S93>N(- - -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c294>t|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>c,V101>T(- - -)|c309>t| c314>t,A105>V(+ - +)|a318>g|g319>c|a320>t| 317 (320) 223 (226) 175 48 (51) 18 (21) 30 9 (10) 5 9 5 5 1 3 (4) 3 6 (7) 0 0 2 78 5 4 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 18 6 1 5 0 1 1 1 1 0 2 0 0 0 0 0 51 51 44 7 2 5 0 2 0 0 1 1 0 0 1 0 0 2 30 24 16 8 5 3 1 0 0 1 1 0 1 2 2 0 0 0 117 114 89 25 9 16 8 2 7 2 2 0 0 1 3 0 0 0 5 (8) 5 4 1 (4) 0 (3) 1 0 (1) 0 1 0 0 0 0 (1) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctcc ggtttcatcttcagtcacttcagc atgaactgggtccgccaggctccaggaaagggcctggagtggatcgccgac atcagtagttcaagtgcatacatc acctatgcagattcagtcaggggccgattcgtcgtctccagggacgacgccaaggactccctgtacttgcaaatggacaacctgggagtcgacgatacggccacctattattgt gtgaggctcttcaccagcgatggagtgagaggcgactac VRLFTSDGVRGDY IGHV3-21 IGHD5-12 IGHJ4 ggtttcatcttcagtcacttcagc atgaactgggtccgccaggctccaggaaagggcctggagtggatcgccgac atcagtagttcaagtgcatacatc acctatgcagattcagtcaggggccgattcgtcgtctccagggacgacgccaaggactccctgtacttgcaaatggacaacctgggagtcgacgatacggccacctattattgt gtgaggctcttcaccagcgatggagtgagaggcgactac IGG3 46 213 26 20 13 20 13 26 11 6 21 8 JY8QFUQ01B1W4F 9009 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 933 91.82 202/220 nt 91.36 201/220 nt Homsap IGHJ3*01 F (see comment) 160 80 40/50 nt Homsap IGHD6-13*01 F 3 8 8 14 8.8.14 [2.17.38.11] CARDLRARLWHPMDVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 14 to 14 in the user submitted sequence), (cause frameshift); agcctctacattcGccctttagcaactatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtctcatccatgggtagtagtagtatttacatatattacgcagactcagtgaagggccgattcaccatctccagagacgacgccaagagttcactgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgtgcgagagacttaagggctcgactatggcatccgatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [295] g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>c,T29>P(+ + -)|c90>t|t105>c|g107>a,S36>N(- - -)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)|a119>g,N40>S(- - -)|t168>g,I56>M(+ + -)|a169>g,S57>G(+ + -)|g188>t,S63>I(- - -)|c198>t|a244>g,N82>D(+ + -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>c,T29>P(+ + -)|c90>t|t105>c|g107>a,S36>N(- - -)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)| a119>g,N40>S(- - -)| t168>g,I56>M(+ + -)|a169>g,S57>G(+ + -)|g188>t,S63>I(- - -)| c198>t|a244>g,N82>D(+ + -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 228 210 18 3 15 5 4 3 1 1 0 0 0 2 0 1 1 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 16 8 2 6 1 2 1 1 1 0 0 0 2 0 0 0 51 51 50 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 0 0 1 1 117 114 108 6 1 5 2 2 2 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct acattcccctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcatcc atgggtagtagtagtatttacata tattacgcagactcagtgaagggccgattcaccatctccagagacgacgccaagagttcactgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgt gcgagagacttaagggctcgactatggcatccgatggacgtc ARDLRARLWHPMDV IGHV3-21 IGHD6-13 IGHJ3 acattcccctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcatcc atgggtagtagtagtatttacata tattacgcagactcagtgaagggccgattcaccatctccagagacgacgccaagagttcactgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgt gcgagagacttaagggctcgactatggcatccgatggacgtc IGG1 18 213 13 5 7 10 6 8 1 2 6 9 JY8QFUQ01B1X3U 21466 productive (see comment) Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 954 93.55 203/217 nt 93.09 202/217 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD4-17*01 F 2 8 8 18 8.8.18 [1.17.38.11] CSTGPSDLSRDYGDYGFDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 58 of V-REGION: 'CATGATGGAATG' (12 nucleotides at position 86 to 97 in the user submitted sequence), (do not cause frameshift); atctggattcaccttcagaaactatgccatgcattgggtccgccaggctccaggcaaggggctggagtgggtggcacttatatggCATGATGGAATGtttgatggaagaaatgaatattatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaatacggtgtatctgcaaatggacagcctgagagccgaggacacggctttgtattactgttcgacaggaccctcagaccttagtcgtgattacggtgactacggatttgactactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [314] g75>a|t105>a,S35>R(- - -)|g107>a,S36>N(- - -)|g113>c,G38>A(- + -)|c120>t|g163>c,V55>L(+ - +)|a173>t,Y58>F(- + -)|t189>a,S63>R(- - -)|a193>g,K65>E(+ - -)|c198>t|c255>t|c259>g,L87>V(+ - +)|a274>g,N92>D(+ + -)|g301>t,V101>L(+ - +)|g313>t,A105>S(- + -)|g317>c,R106>T(- - -)| g75>a| t105>a,S35>R(- - -)|g107>a,S36>N(- - -)|g113>c,G38>A(- + -)| c120>t|g163>c,V55>L(+ - +)| a173>t,Y58>F(- + -)|t189>a,S63>R(- - -)|a193>g,K65>E(+ - -)| c198>t|c255>t|c259>g,L87>V(+ - +)|a274>g,N92>D(+ + -)|g301>t,V101>L(+ - +)| g313>t,A105>S(- + -)|g317>c,R106>T(- - -)|a320>g| 319 (320) 224 (225) 208 16 (17) 4 (5) 12 2 (3) 2 3 0 0 0 1 2 3 1 2 0 78 4 3 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 1 1 0 0 0 51 51 49 2 1 1 0 0 1 0 0 0 0 0 1 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 1 1 0 0 0 0 117 114 109 5 2 3 1 0 2 0 0 0 0 0 0 1 1 0 7 (8) 7 5 2 (3) 0 (1) 2 0 (1) 0 0 0 0 0 0 0 1 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| atct ggattcaccttcagaaactatgcc atgcattgggtccgccaggctccaggcaaggggctggagtgggtggcactt atatggtttgatggaagaaatgaa tattatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaatacggtgtatctgcaaatggacagcctgagagccgaggacacggctttgtattactgt tcgacaggaccctcagaccttagtcgtgattacggtgactacggatttgactac STGPSDLSRDYGDYGFDY IGHV3-33 IGHD4-17 IGHJ4 ggattcaccttcagaaactatgcc atgcattgggtccgccaggctccaggcaaggggctggagtgggtggcactt atatggtttgatggaagaaatgaa tattatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaatacggtgtatctgcaaatggacagcctgagagccgaggacacggctttgtattactgt tcgacaggaccctcagaccttagtcgtgattacggtgactacggatttgactac IGA1 13 213 6 7 4 8 2 5 3 0 4 6 JY8QFUQ01B1Y16 11996 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 909 91.24 198/217 nt NA Homsap IGHJ5*02 F 237 96.08 49/51 nt Homsap IGHD2-2*01 F 1 8 8 16 8.8.16 [1.17.38.11] CAKDPQQLLAEVNWFDPW in-frame - ctctggattcacttttagaagttatgccatgagttgggtccgccaggctccagggaaggggctagagtgggtctcatctatcagtggtaatggtgataagacaaagtacgcagactccgtgaagggccggttcaccatctccagggacaattttaagaatacgttgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattattgtgcgaaagatcctcagcagctattggcagaggtaaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [296] c87>t|c105>a,S35>R(- - -)|c108>t|c120>t|g150>a|g163>t,A55>S(- + -)|t168>c|g176>a,S59>N(- - -)|g188>a,G63>D(- - -)|g191>a,S64>K(- - -)|c192>g,S64>K(- - -)|t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|a240>g|c248>t,S83>F(- - -)|c249>t,S83>F(- - -)|c255>t|c259>t|c309>t| c87>t|c105>a,S35>R(- - -)|c108>t| c120>t|g150>a|g163>t,A55>S(- + -)| t168>c|g176>a,S59>N(- - -)|g188>a,G63>D(- - -)|g191>a,S64>K(- - -)|c192>g,S64>K(- - -)| t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|a240>g|c248>t,S83>F(- - -)|c249>t,S83>F(- - -)|c255>t|c259>t|c309>t| 320 225 206 19 9 10 1 4 8 1 0 1 0 1 0 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 2 0 0 1 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 0 0 0 1 0 30 24 19 5 1 4 0 3 0 1 0 0 0 0 0 1 0 0 117 114 106 8 4 4 1 0 5 0 0 0 0 1 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacttttagaagttatgcc atgagttgggtccgccaggctccagggaaggggctagagtgggtctcatct atcagtggtaatggtgataagaca aagtacgcagactccgtgaagggccggttcaccatctccagggacaattttaagaatacgttgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattattgt gcgaaagatcctcagcagctattggcagaggtaaactggttcgacccc AKDPQQLLAEVNWFDP IGHV3-23 IGHD2-2 IGHJ5 ggattcacttttagaagttatgcc atgagttgggtccgccaggctccagggaaggggctagagtgggtctcatct atcagtggtaatggtgataagaca aagtacgcagactccgtgaagggccggttcaccatctccagggacaattttaagaatacgttgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattattgt gcgaaagatcctcagcagctattggcagaggtaaactggttcgacccc IGA1 19 213 14 5 12 16 2 3 6 3 5 5 JY8QFUQ01B20H0 21400 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 1053 98.62 214/217 nt 98.16 213/217 nt Homsap IGHJ2*01 F 247 96.23 51/53 nt Homsap IGHD5-24*01 ORF 2 8 8 11 8.8.11 [1.17.38.11] CARSQGFWYFDLW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 91 of V-REGION: 'A' (1 nucleotides at position 176 to 176 in the user submitted sequence), (cause frameshift); ctctggattcaccttcagtagctatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtcgcatccattagtagtagtagtggttacatatactacgcagactcagtgaagggccgattcaccgtctccagagacaacgccaagaactcactgtatctgcaaAatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagatcgcagggattctggtacttcgatctctggggccgtggcaccctggtcactgtctcctcagcttccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG3 100 100 [283] t160>g,S54>A(- + -)|a187>g,S63>G(+ + -)|a232>g,I78>V(+ - +)| t160>g,S54>A(- + -)| a187>g,S63>G(+ + -)| a232>g,I78>V(+ - +)| g319>t|a320>c| 318 (320) 223 (225) 220 3 (5) 0 (2) 3 2 0 0 0 0 (1) 0 0 0 0 0 0 (1) 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 30 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 117 114 113 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtcgcatcc attagtagtagtagtggttacata tactacgcagactcagtgaagggccgattcaccgtctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagatcgcagggattctggtacttcgatctc ARSQGFWYFDL IGHV3-21 IGHD5-24 IGHJ2 ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtcgcatcc attagtagtagtagtggttacata tactacgcagactcagtgaagggccgattcaccgtctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagatcgcagggattctggtacttcgatctc IGG3 3 213 2 1 0 0 2 3 0 0 2 1 JY8QFUQ01B27X3 3312 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 995 95.41 208/218 nt 94.95 207/218 nt Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD2-15*01 F 3 8 8 17 8.8.17 [2.16.38.11] CARDTLEVVSNMDDAFDFW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 60 in the user submitted sequence), (cause frameshift) gcctctggattcaccttcagtcgttatagcatgaactgggtccgccaggctccagggaagggctggagtgggtctcatacattagtaggactactactgacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatatttctgtgcgagagatactctagaggtggtgtctaatatggatgatgcttttgatttctggggccaagggacagtggtcaatgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [300] a106>c,S36>R(- - -)|c108>t,S36>R(- - -)|c164>a,S55>Y(+ - -)|t174>g,S58>R(- - -)|g176>c,S59>T(+ - +)|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t190>g,Y64>D(- - -)|g303>a|a308>t,Y103>F(- + -)| a106>c,S36>R(- - -)|c108>t,S36>R(- - -)| c164>a,S55>Y(+ - -)| t174>g,S58>R(- - -)|g176>c,S59>T(+ - +)|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t190>g,Y64>D(- - -)| g303>a|a308>t,Y103>F(- + -)| 320 226 216 10 1 9 0 1 1 0 1 1 1 0 3 0 0 2 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 1 0 1 0 0 0 0 0 0 0 51 50 49 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 30 24 19 5 0 5 0 0 0 0 0 0 0 0 3 0 0 2 117 114 112 2 1 1 0 1 0 0 0 0 1 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcaccttcagtcgttatagc atgaactgggtccgccaggctccagggaagggctggagtgggtctcatac attagtaggactactactgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatatttctgt gcgagagatactctagaggtggtgtctaatatggatgatgcttttgatttc ARDTLEVVSNMDDAFDF IGHV3-21 IGHD2-15 IGHJ3 ggattcaccttcagtcgttatagc atgaactgggtccgccaggctccagggaagggctggagtgggtctcatac attagtaggactactactgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatatttctgt gcgagagatactctagaggtggtgtctaatatggatgatgcttttgatttc IGA1 10 212 2 8 2 6 0 4 1 0 2 7 JY8QFUQ01B2ARX 6635 productive Homsap IGHV3-33*03 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-18*01 F 1 8 8 13 8.8.13 [1.17.38.11] CARSDLSMVRDLDYW in-frame + gtctggattcaccttcagtacctatggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatgatttggtatgatggaagccttacatattatgaagactccgtgaagggccgattcaccatctccagagacaactccaagaacacgctgtttctgcaaatgaatagcctgagagtcgaggacacggctctgtattactgtgcgagatccgacctctctatggttagagatcttgactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] g107>c,S36>T(+ - +)|g163>a,V55>M(+ - -)|t165>g,V55>M(+ - -)|a168>t|t189>c|a190>c,N64>L(- - -)|a191>t,N64>L(- - -)|a194>c,K65>T(- - -)|c198>t|c203>a,A68>E(- - -)|a263>t,Y88>F(- + -)|c276>t|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)| g107>c,S36>T(+ - +)| g163>a,V55>M(+ - -)|t165>g,V55>M(+ - -)| a168>t|t189>c|a190>c,N64>L(- - -)|a191>t,N64>L(- - -)|a194>c,K65>T(- - -)| c198>t|c203>a,A68>E(- - -)|a263>t,Y88>F(- + -)|c276>t|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)| a317>g,K106>R(+ + +)|g319>t|a320>c| 316 (320) 221 (225) 207 (208) 14 (17) 4 (6) 10 (11) 0 (1) 1 3 1 2 (3) 1 3 0 2 0 0 (1) 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 30 24 19 5 2 3 0 0 0 1 2 0 2 0 0 0 0 0 117 114 108 6 2 4 0 0 3 0 0 1 1 0 1 0 0 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|aact,244-247(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcaccttcagtacctatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatg atttggtatgatggaagccttaca tattatgaagactccgtgaagggccgattcaccatctccagagacaactccaagaacacgctgtttctgcaaatgaatagcctgagagtcgaggacacggctctgtattactgt gcgagatccgacctctctatggttagagatcttgactat ARSDLSMVRDLDY IGHV3-33 IGHD5-18 IGHJ4 ggattcaccttcagtacctatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatg atttggtatgatggaagccttaca tattatgaagactccgtgaagggccgattcaccatctccagagacaactccaagaacacgctgtttctgcaaatgaatagcctgagagtcgaggacacggctctgtattactgt gcgagatccgacctctctatggttagagatcttgactat IGA1 14 213 5 9 4 7 1 7 2 2 6 4 JY8QFUQ01B2D9Z 18500 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 968 94.04 205/218 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD5-12*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARGPRSRGYGMDVW in-frame + cctctacattcgcctttagcaactatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtgtcagatattagtgggagtggtgttagcacacactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgtgcgagagggcctcgatcgcgcggctacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [289] g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)|c159>g|c164>a,A55>D(- - -)|t174>g|g188>t,G63>V(- - -)|t196>c,Y66>H(+ - -)|a204>g|a214>c,K72>Q(+ - -)|c249>g|a285>t,R95>S(- - -)|a317>g,K106>R(+ + +)| g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)| c159>g|c164>a,A55>D(- - -)| t174>g|g188>t,G63>V(- - -)| t196>c,Y66>H(+ - -)|a204>g|a214>c,K72>Q(+ - -)|c249>g|a285>t,R95>S(- - -)| a317>g,K106>R(+ + +)|a320>g| 319 (320) 225 (226) 211 14 (15) 4 (5) 10 3 (4) 2 0 1 1 1 1 0 1 2 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 2 0 0 0 0 0 0 1 0 0 0 51 51 49 2 1 1 0 0 0 0 0 1 0 0 0 1 0 0 30 24 22 2 1 1 0 0 0 0 0 0 0 0 0 0 1 1 117 114 109 5 2 3 1 0 0 1 1 0 1 0 0 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 1 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct acattcgcctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtgtcagat attagtgggagtggtgttagcaca cactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgt gcgagagggcctcgatcgcgcggctacggtatggacgtc ARGPRSRGYGMDV IGHV3-23 IGHD5-12 IGHJ6 acattcgcctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtgtcagat attagtgggagtggtgttagcaca cactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgt gcgagagggcctcgatcgcgcggctacggtatggacgtc IGG1 13 213 5 8 2 7 3 6 3 1 4 5 JY8QFUQ01B2ERL 5779 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 837 87.56 190/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-15*01 F 1 8 8 11 8.8.11 [1.17.38.11] CARDGEWEMLDHW in-frame + ctccggattcaccttcagtaggtattggatgtattgggtccgccaagctccaggcaaggggctggtgtgggtctcacgcattaaaagtgatgggacgagtgcaacctacgcggactccgtgaagggccgattcaccacctccagagacaacgccaaggaaacgatgtatctgcacatgaacagcctgagagtcgacgacacggcgacatatttttgtgcaagagacggggagtgggagatgcttgaccactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA1 71 100 [281] t78>c|c108>g,S36>R(- - -)|c111>t|c118>t,H40>Y(+ - -)|c120>t,H40>Y(+ - -)|g141>c|t165>c|t171>a,N57>K(+ - -)|g188>c,S63>T(+ - +)|t189>g,S63>T(+ - +)|c192>t|a193>g,T65>A(- - -)|g197>c,S66>T(+ - +)|t233>c,I78>T(- - -)|a253>g,N85>E(+ - -)|c255>a,N85>E(+ - -)|c259>a,L87>M(+ + -)|a270>c,Q90>H(- + -)|t279>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t300>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| t78>c| c108>g,S36>R(- - -)|c111>t| c118>t,H40>Y(+ - -)|c120>t,H40>Y(+ - -)|g141>c|t165>c| t171>a,N57>K(+ - -)|g188>c,S63>T(+ - +)|t189>g,S63>T(+ - +)|c192>t|a193>g,T65>A(- - -)| g197>c,S66>T(+ - +)|t233>c,I78>T(- - -)|a253>g,N85>E(+ - -)|c255>a,N85>E(+ - -)|c259>a,L87>M(+ + -)|a270>c,Q90>H(- + -)|t279>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t300>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| 320 225 198 27 7 20 2 2 6 5 1 2 1 1 4 1 0 2 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 1 0 0 0 0 0 0 1 0 0 51 51 47 4 2 2 0 0 2 1 0 0 0 0 1 0 0 0 30 24 19 5 1 4 1 0 1 0 0 0 0 1 1 0 0 1 117 114 99 15 2 13 1 2 2 3 1 2 1 0 2 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcaccttcagtaggtattgg atgtattgggtccgccaagctccaggcaaggggctggtgtgggtctcacgc attaaaagtgatgggacgagtgca acctacgcggactccgtgaagggccgattcaccacctccagagacaacgccaaggaaacgatgtatctgcacatgaacagcctgagagtcgacgacacggcgacatatttttgt gcaagagacggggagtgggagatgcttgaccac ARDGEWEMLDH IGHV3-74 IGHD2-15 IGHJ4 ggattcaccttcagtaggtattgg atgtattgggtccgccaagctccaggcaaggggctggtgtgggtctcacgc attaaaagtgatgggacgagtgca acctacgcggactccgtgaagggccgattcaccacctccagagacaacgccaaggaaacgatgtatctgcacatgaacagcctgagagtcgacgacacggcgacatatttttgt gcaagagacggggagtgggagatgcttgaccac IGA1 26 213 14 12 8 15 6 11 4 2 15 5 JY8QFUQ01B2GU8 20995 productive (see comment) Homsap IGHV4-59*03 F 935 93.02 200/215 nt 91.63 197/215 nt Homsap IGHJ5*02 F (see comment) 201 88.24 45/51 nt Homsap IGHD3-22*01 F 2 8 7 16 8.7.16 [1.17.38.11] CARGHFDSSGYSNPLDHW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in CDR1-IMGT at codon 38 of V-REGION: 'G' (1 nucleotides at position 28 to 28 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 50 to 50 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 59 of V-REGION: 'AGCGGG' (6 nucleotides at position 92 to 97 in the user submitted sequence), (do not cause frameshift); tctctggtgtctccatcagtagtttctGactggagttggatgaggcagcCccccagggaggggactggagtggattggatatgtccatggcAGCGGGagtgggagcaccaactccaacccctccctcaagagtcgagtcaccatgtcagtggacacgtccaagaaccaattctccctgaagctgggctctgtgaccgctgcggacacggccgtgtattactgtgcgagaggacattttgatagtagtggttattccaaccctttagaccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [302] g83>t,G28>V(- - -)|a110>t,Y37>F(- + -)|c120>t|c126>g,I42>M(+ + -)|c127>a|a143>g,K48>R(+ + +)|g162>a|a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|t172>g,Y58>G(+ - -)|a173>g,Y58>G(+ - -)|a200>c,Y67>S(+ - -)|a234>g,I78>M(+ + -)|a240>g|a274>g,S92>G(+ + -)| g83>t,G28>V(- - -)|a110>t,Y37>F(- + -)| c120>t|c126>g,I42>M(+ + -)|c127>a|a143>g,K48>R(+ + +)|g162>a| a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|t172>g,Y58>G(+ - -)|a173>g,Y58>G(+ - -)| a200>c,Y67>S(+ - -)|a234>g,I78>M(+ + -)|a240>g|a274>g,S92>G(+ + -)| 315 218 203 15 4 11 6 1 1 1 1 1 1 0 0 1 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 0 0 1 0 51 51 46 5 3 2 1 1 1 0 0 1 0 0 0 1 0 0 30 21 17 4 0 4 2 0 0 1 0 0 0 0 0 0 0 1 117 114 110 4 1 3 3 0 0 0 1 0 0 0 0 0 0 0 3 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,257-258(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgtctccatcagtagtttctac tggagttggatgaggcagcccccagggaggggactggagtggattggatat gtccatggcagtgggagcacc aactccaacccctccctcaagagtcgagtcaccatgtcagtggacacgtccaagaaccaattctccctgaagctgggctctgtgaccgctgcggacacggccgtgtattactgt gcgagaggacattttgatagtagtggttattccaaccctttagaccac ARGHFDSSGYSNPLDH IGHV4-59 IGHD3-22 IGHJ5 ggtgtctccatcagtagtttctac tggagttggatgaggcagcccccagggaggggactggagtggattggatat gtccatggcagtgggagcacc aactccaacccctccctcaagagtcgagtcaccatgtcagtggacacgtccaagaaccaattctccctgaagctgggctctgtgaccgctgcggacacggccgtgtattactgt gcgagaggacattttgatagtagtggttattccaaccctttagaccac IGA1 15 210 9 6 2 5 7 10 4 0 5 6 JY8QFUQ01B2JOM 8546 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 943 93.9 200/213 nt 92.96 198/213 nt Homsap IGHJ4*03 F 213 93.75 45/48 nt Homsap IGHD1-26*01 F 1 7 7 11 7.7.11 [1.17.38.11] CAKEGNLGYFDDW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 30 of V-REGION: 1 nucleotides (from position 16 in the user submitted sequence), (cause frameshift)in CDR2-IMGT, from codon 56 of V-REGION: 3 nucleotides (from position 81 in the user submitted sequence), (do not cause frameshift) ctctggattcaccttagcagccatcccatgagctgggtccgccaggctccgggaaaagggctggagtggatctcagctttcgttcgtagtggtaacacatactacgtagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaaagaaggtaatctgggctactttgacgactggggtcaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [277] t109>c,Y37>H(+ - -)|g112>c,A38>P(- - -)|a138>g|g141>a|g144>a|g157>a,V53>I(+ - +)|a166>t|t171>c|g173>t,G58>V(- - -)|a175>c,S59>R(- - -)|g184>a,G62>S(+ + -)|g191>a,S64>N(- - -)|c203>t,A68>V(+ - +)| t109>c,Y37>H(+ - -)|g112>c,A38>P(- - -)| a138>g|g141>a|g144>a|g157>a,V53>I(+ - +)| a166>t|t171>c|g173>t,G58>V(- - -)|a175>c,S59>R(- - -)|g184>a,G62>S(+ + -)|g191>a,S64>N(- - -)| c203>t,A68>V(+ - +)| 320 221 208 13 5 8 1 5 1 2 1 0 1 0 1 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 21 2 0 2 0 0 0 1 0 0 0 0 1 0 0 0 51 51 47 4 3 1 1 3 0 0 0 0 0 0 0 0 0 0 30 21 15 6 2 4 0 2 0 1 1 0 1 0 0 0 1 0 117 114 113 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttagcagccatccc atgagctgggtccgccaggctccgggaaaagggctggagtggatctcagct ttcgttcgtagtggtaacaca tactacgtagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaagaaggtaatctgggctactttgacgac AKEGNLGYFDD IGHV3-23 IGHD1-26 IGHJ4 ggattcaccttagcagccatccc atgagctgggtccgccaggctccgggaaaagggctggagtggatctcagct ttcgttcgtagtggtaacaca tactacgtagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaagaaggtaatctgggctactttgacgac IGA2 13 209 9 4 6 8 3 5 3 2 2 6 JY8QFUQ01B2LDP 22356 productive (see comment) Homsap IGHV3-7*01 F 885 90.65 194/214 nt 89.72 192/214 nt Homsap IGHJ5*02 F (see comment) 192 86.27 44/51 nt Homsap IGHD4-23*01 ORF 3 8 8 12 8.8.12 [0.17.38.11] CARGTSVDPGIDHW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT at codon 30 of V-REGION: 'T' (1 nucleotides at position 13 to 13 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 48 of V-REGION: 'G' (1 nucleotides at position 55 to 55 in the user submitted sequence), (cause frameshift); tggattcaccttTtagtaattattggatgcactgggtccgccaggtttcagggaGgggggctggagtgcgtggccaccataaacgaagatggaagtgagagacactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactctctgtttttgcagatgaacagcctgagagccgaggacacggctgtctactactgtgcgagagggacctcagtggacccggggattgaccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccca NA IGA2 93 100 [283, 284] g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)|c134>t,A45>V(+ - +)|c136>t,P46>S(+ - -)|a143>g,K48>R(+ + +)|g156>c,W52>C(+ - -)|a164>c,N55>T(- + -)|g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)|t196>c,Y66>H(+ - -)|a232>g,I78>V(+ - +)|a258>t|a263>t,Y88>F(- + -)|c265>t|a270>g|g303>c|t306>c| g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)|c134>t,A45>V(+ - +)|c136>t,P46>S(+ - -)|a143>g,K48>R(+ + +)|g156>c,W52>C(+ - -)|a164>c,N55>T(- + -)| g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)| t196>c,Y66>H(+ - -)|a232>g,I78>V(+ - +)|a258>t|a263>t,Y88>F(- + -)|c265>t|a270>g|g303>c|t306>c| a320>g| 319 (320) 221 (222) 201 20 (21) 5 (6) 15 4 (5) 2 4 2 2 0 2 0 3 1 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 51 51 44 7 0 7 1 1 2 0 2 0 0 0 1 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 1 1 0 0 117 114 106 8 5 3 2 0 1 2 0 0 2 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t ggattcacctttagtaattattgg atgcactgggtccgccaggtttcagggagggggctggagtgcgtggccacc ataaacgaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactctctgtttttgcagatgaacagcctgagagccgaggacacggctgtctactactgt gcgagagggacctcagtggacccggggattgaccac ARGTSVDPGIDH IGHV3-7 IGHD4-23 IGHJ5 ggattcacctttagtaattattgg atgcactgggtccgccaggtttcagggagggggctggagtgcgtggccacc ataaacgaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactctctgtttttgcagatgaacagcctgagagccgaggacacggctgtctactactgt gcgagagggacctcagtggacccggggattgaccac IGA2 20 213 12 8 6 10 6 10 5 0 10 5 JY8QFUQ01B2NHE 19332 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 903 91.59 196/214 nt 91.12 195/214 nt Homsap IGHJ3*02 F 214 92 46/50 nt Homsap IGHD2-21*01 F 2 8 7 15 8.7.15 [1.17.38.11] CTRELRLAAARDGVDIW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 60 to 60 in the user submitted sequence), (cause frameshift); ttctgggttcaccgtcagtagcaagttcatgagctgggtccgccaggctccagggaaggGggctggagtgggtctcaattacttatcctgatggtactacatattatggagactccgtgaagggccgattcaccatctccagagacaattccaagaacacactggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgtacgagagaacttaggctggctgctgcgcgtgatggtgttgacatctggggccaggggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [291] c75>t|c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)|g163>a,V55>I(+ - +)|t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|c198>t|c201>t|c203>g,A68>G(- + -)|g258>a|t262>g,Y88>D(- - -)|g303>t|c309>t|g313>a,A105>T(- - -)| c75>t| c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)| g163>a,V55>I(+ - +)| t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)| c198>t|c201>t|c203>g,A68>G(- + -)|g258>a|t262>g,Y88>D(- - -)|g303>t|c309>t| g313>a,A105>T(- - -)| 320 222 203 19 6 13 0 4 6 1 1 0 1 0 2 2 1 1 78 4 3 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 21 14 7 0 7 0 1 2 1 1 0 0 0 2 0 0 0 117 114 107 7 5 2 0 1 3 0 0 0 0 0 0 1 1 1 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ttct gggttcaccgtcagtagcaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaatt acttatcctgatggtactaca tattatggagactccgtgaagggccgattcaccatctccagagacaattccaagaacacactggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttaggctggctgctgcgcgtgatggtgttgacatc TRELRLAAARDGVDI IGHV3-53 IGHD2-21 IGHJ3 gggttcaccgtcagtagcaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaatt acttatcctgatggtactaca tattatggagactccgtgaagggccgattcaccatctccagagacaattccaagaacacactggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttaggctggctgctgcgcgtgatggtgttgacatc IGA2 17 210 9 8 8 13 1 4 5 0 3 9 JY8QFUQ01B2PNO 6660 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 3 8 8 19 8.8.19 [1.17.38.11] CAKDVSTRITMRVVVPALDYW in-frame + gtctggattcacgtttagaaactatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaagtattagtgataatggtgacagcatattccacgcagactccgtgaagggccggttcaccatcaccagagacaactctaagaacatcctgtatctgcacatgaacagcctgagagccgaggactcggccgtatattactgtgcgaaggatgtttccacgcgtattactatgagagttgtggtgccagcccttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaaccgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 89 [305, 306] c75>g|c87>g|c105>a,S35>R(- - -)|g107>a,S36>N(- - -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|g173>a,G58>D(- - -)|g176>a,S59>N(- - -)|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|c194>t,T65>I(- - -)|a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|t235>a,S79>T(+ - +)|t246>c|c249>t|c257>t,T86>I(- - -)|g258>c,T86>I(- - -)|a270>c,Q90>H(- + -)|a295>t,T99>S(+ - +)|a318>g| c75>g| c87>g|c105>a,S35>R(- - -)|g107>a,S36>N(- - -)| g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| g173>a,G58>D(- - -)|g176>a,S59>N(- - -)|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|c194>t,T65>I(- - -)| a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|t235>a,S79>T(+ - +)|t246>c|c249>t|c257>t,T86>I(- - -)|g258>c,T86>I(- - -)|a270>c,Q90>H(- + -)|a295>t,T99>S(+ - +)| a318>g| 320 225 204 21 5 16 1 5 3 3 1 1 2 1 1 3 0 0 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 21 3 1 2 0 1 0 0 0 1 0 0 0 1 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 0 1 0 0 30 24 19 5 0 5 0 3 1 1 0 0 0 0 0 0 0 0 117 114 105 9 2 7 0 0 2 2 1 0 2 1 1 0 0 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcacgtttagaaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaagt attagtgataatggtgacagcata ttccacgcagactccgtgaagggccggttcaccatcaccagagacaactctaagaacatcctgtatctgcacatgaacagcctgagagccgaggactcggccgtatattactgt gcgaaggatgtttccacgcgtattactatgagagttgtggtgccagcccttgactac AKDVSTRITMRVVVPALDY IGHV3-23 IGHD3-22 IGHJ4 ggattcacgtttagaaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaagt attagtgataatggtgacagcata ttccacgcagactccgtgaagggccggttcaccatcaccagagacaactctaagaacatcctgtatctgcacatgaacagcctgagagccgaggactcggccgtatattactgt gcgaaggatgtttccacgcgtattactatgagagttgtggtgccagcccttgactac IGA1 19 213 11 8 8 12 3 7 2 1 9 7 JY8QFUQ01B325I 14251 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*02 F or Homsap IGHV4-39*05 F (see comment) 767 83.26 184/221 nt 82.81 183/221 nt Homsap IGHJ4*02 F (see comment) 141 77.08 37/48 nt Homsap IGHD3-3*01 F 2 10 7 12 10.7.12 [1.17.38.11] CARRWSGTYPFASW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (83,26% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 45 of V-REGION: 'T' (1 nucleotides at position 54 to 54 in the user submitted sequence), (cause frameshift); tctctggtggctcactcagaagtagtagtcaccattggggctggattcgtcagTccccccgggaaggggctggagtggcttgggactgtcgactttcgtgggaccacccactacaacccgtccctcatgggtcgactcacgatatccgtcgacgcgcccaagagtcaaatgtccctgcacttgagctctgtgaccgccgcagacacggctttttacttctgtgcgagacgctggagtgggacttatccgtttgcctcctggggccagggcatcccggtcatcgtctccccagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtccgtggaactcag NA IGG4 100 88 [290] c87>a|a88>c,I30>L(+ + +)|c93>a,S31>R(- - -)|t109>c,Y37>H(+ - -)|t112>c,Y38>H(+ - -)|c114>t,Y38>H(+ - -)|c126>t|c129>t|a138>c|a157>c,I53>L(+ + +)|g164>c,S55>T(+ - +)|a166>g,I56>V(+ - +)|t169>g,Y57>D(- - -)|t171>c,Y57>D(- - -)|a173>t,Y58>F(- + -)|a175>c,S59>R(- - -)|g191>c,S64>T(+ - +)|t196>c,Y66>H(+ - -)|a215>t,K72>M(- + -)|a220>g,S74>G(+ + -)|g226>c,V76>L(+ - +)|c231>g|a240>c|a244>g,T82>A(- - -)|t247>c,S83>P(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|t259>a,F87>M(+ - -)|c261>g,F87>M(+ - -)|a268>c,K90>H(- - +)|g270>c,K90>H(- - +)|c271>t|g301>t,V101>F(+ - -)|g303>t,V101>F(+ - -)|t306>c|a308>t,Y103>F(- + -)| c87>a|a88>c,I30>L(+ + +)|c93>a,S31>R(- - -)|t109>c,Y37>H(+ - -)|t112>c,Y38>H(+ - -)|c114>t,Y38>H(+ - -)| c126>t|c129>t|a138>c|a157>c,I53>L(+ + +)|g164>c,S55>T(+ - +)| a166>g,I56>V(+ - +)|t169>g,Y57>D(- - -)|t171>c,Y57>D(- - -)|a173>t,Y58>F(- + -)|a175>c,S59>R(- - -)|g191>c,S64>T(+ - +)| t196>c,Y66>H(+ - -)|a215>t,K72>M(- + -)|a220>g,S74>G(+ + -)|g226>c,V76>L(+ - +)|c231>g|a240>c|a244>g,T82>A(- - -)|t247>c,S83>P(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|t259>a,F87>M(+ - -)|c261>g,F87>M(+ - -)|a268>c,K90>H(- - +)|g270>c,K90>H(- - +)|c271>t|g301>t,V101>F(+ - -)|g303>t,V101>F(+ - -)|t306>c|a308>t,Y103>F(- + -)| a320>g| 319 (320) 228 (229) 191 37 (38) 9 (10) 28 4 (5) 1 5 6 6 2 3 1 4 2 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 1 5 0 0 1 2 1 2 0 0 0 0 0 0 51 51 46 5 3 2 0 0 2 0 2 0 0 0 1 0 0 0 30 21 15 6 0 6 1 0 0 1 1 0 1 0 1 0 0 1 117 114 94 20 5 15 3 1 2 3 2 0 2 1 2 2 2 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctcactcagaagtagtagtcaccat tggggctggattcgtcagccccccgggaaggggctggagtggcttgggact gtcgactttcgtgggaccacc cactacaacccgtccctcatgggtcgactcacgatatccgtcgacgcgcccaagagtcaaatgtccctgcacttgagctctgtgaccgccgcagacacggctttttacttctgt gcgagacgctggagtgggacttatccgtttgcctcc ARRWSGTYPFAS IGHV4-39 IGHD3-3 IGHJ4 ggtggctcactcagaagtagtagtcaccat tggggctggattcgtcagccccccgggaaggggctggagtggcttgggact gtcgactttcgtgggaccacc cactacaacccgtccctcatgggtcgactcacgatatccgtcgacgcgcccaagagtcaaatgtccctgcacttgagctctgtgaccgccgcagacacggctttttacttctgt gcgagacgctggagtgggacttatccgtttgcctcc IGG4 37 216 16 21 6 16 10 21 8 1 17 11 JY8QFUQ01B3AX2 20788 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 851 88.07 192/218 nt 87.61 191/218 nt Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD3-9*01 F 2 8 8 21 8.8.21 [1.17.38.11] CVREGIEYYEISTGQYYYDMDVW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'A' (1 nucleotides at position 14 to 14 in the user submitted sequence), (cause frameshift); cctctggattcacAttttagcggctatggcatgagctgggtccgccaggctgcagggaaggggctggaatgggtctcatttattagtggtaagagcggcaacatatactatgcagaccccgtgaagggccggttcaccatctccagagacaactccaagaataggctcttcctgcagatgaacagcctgagagtcgaggacacggccaaatattactgtgtgagagagggcatagagtattacgaaatctcgactggacagtactactacgatatggacgtctggggccaggggaccacggtcaccgtcctctctgcatcccccgaccagccccaaggtcttccccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [315, 314] c87>t|a106>g,S36>G(+ + -)|c113>g,A38>G(- + -)|c136>g,P46>A(- - -)|g153>a|g163>t,A55>F(+ - -)|c164>t,A55>F(+ - -)|g176>a,S59>K(- - -)|t177>g,S59>K(- - -)|g184>a,G62>S(+ + -)|t186>c,G62>S(+ + -)|t189>c|g191>a,S64>N(- - -)|c194>t,T65>I(- - -)|c201>t|t208>c,S70>P(+ - -)|t246>c|c255>t|c257>g,T86>R(- - -)|g261>c|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|a270>g|c287>t,A96>V(+ - +)|g301>a,V101>K(- - -)|t302>a,V101>K(- - -)|c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)| c87>t|a106>g,S36>G(+ + -)|c113>g,A38>G(- + -)| c136>g,P46>A(- - -)|g153>a|g163>t,A55>F(+ - -)|c164>t,A55>F(+ - -)| g176>a,S59>K(- - -)|t177>g,S59>K(- - -)|g184>a,G62>S(+ + -)|t186>c,G62>S(+ + -)|t189>c|g191>a,S64>N(- - -)|c194>t,T65>I(- - -)| c201>t|t208>c,S70>P(+ - -)|t246>c|c255>t|c257>g,T86>R(- - -)|g261>c|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|a270>g|c287>t,A96>V(+ - +)|g301>a,V101>K(- - -)|t302>a,V101>K(- - -)| c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)| 320 226 198 28 8 20 3 5 7 5 0 0 1 1 1 3 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 1 0 1 0 0 0 0 0 0 1 0 0 51 51 47 4 1 3 0 1 1 0 0 0 0 0 0 1 1 0 30 24 17 7 1 6 0 3 1 2 0 0 0 0 0 0 0 1 117 114 102 12 5 7 1 1 3 3 0 0 1 1 1 1 0 0 8 8 6 2 0 2 1 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacttttagcggctatggc atgagctgggtccgccaggctgcagggaaggggctggaatgggtctcattt attagtggtaagagcggcaacata tactatgcagaccccgtgaagggccggttcaccatctccagagacaactccaagaataggctcttcctgcagatgaacagcctgagagtcgaggacacggccaaatattactgt gtgagagagggcatagagtattacgaaatctcgactggacagtactactacgatatggacgtc VREGIEYYEISTGQYYYDMDV IGHV3-23 IGHD3-9 IGHJ6 ggattcacttttagcggctatggc atgagctgggtccgccaggctgcagggaaggggctggaatgggtctcattt attagtggtaagagcggcaacata tactatgcagaccccgtgaagggccggttcaccatctccagagacaactccaagaataggctcttcctgcagatgaacagcctgagagtcgaggacacggccaaatattactgt gtgagagagggcatagagtattacgaaatctcgactggacagtactactacgatatggacgtc IGA1 26 213 18 8 11 16 7 10 6 2 10 8 JY8QFUQ01B3AYN 8752 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 928 91.78 201/219 nt 90.87 199/219 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-3*01 F 2 8 8 10 8.8.10 [2.17.38.11] CTKDTFGREDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'G' (1 nucleotides at position 16 to 16 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 41 of V-REGION: 'G' (1 nucleotides at position 39 to 39 in the user submitted sequence), (cause frameshift); gcctctggattcaccGttcagtagatactggatgcactGgggtccgccaggctccagggaaggggctggagtgggtcgcacgtactaatgaagatgggagtactaaaaactacgcggactccgtgaagggccgattcaccatcttcagagacaacaccaagaacacactatatctgcaaatgaacagtctgagagacgaggacacggctgtgtattattgtacaaaagacacgtttgggagagaagactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [282] c108>a,S36>R(- - -)|a132>g|t152>a,V51>E(- + -)|t160>g,S54>A(- + -)|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|c194>a,T65>K(- - -)|g197>a,S66>N(- - -)|c236>t,S79>F(- - -)|g247>a,A83>T(- - -)|g258>a|g261>a|c287>a,A96>D(- - -)|c309>t|g313>a,A105>T(- - -)|g317>a,R106>K(+ + +)| c108>a,S36>R(- - -)| a132>g|t152>a,V51>E(- + -)|t160>g,S54>A(- + -)| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|c194>a,T65>K(- - -)| g197>a,S66>N(- - -)|c236>t,S79>F(- - -)|g247>a,A83>T(- - -)|g258>a|g261>a|c287>a,A96>D(- - -)|c309>t| g313>a,A105>T(- - -)|g317>a,R106>K(+ + +)| 320 227 207 20 4 16 2 7 3 1 0 3 0 2 1 0 0 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 48 3 1 2 1 0 0 0 0 0 0 1 0 0 0 1 30 24 17 7 0 7 1 1 1 1 0 1 0 1 1 0 0 0 117 114 107 7 3 4 0 4 2 0 0 1 0 0 0 0 0 0 8 8 6 2 0 2 0 2 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcaccttcagtagatactgg atgcactgggtccgccaggctccagggaaggggctggagtgggtcgcacgt actaatgaagatgggagtactaaa aactacgcggactccgtgaagggccgattcaccatcttcagagacaacaccaagaacacactatatctgcaaatgaacagtctgagagacgaggacacggctgtgtattattgt acaaaagacacgtttgggagagaagactac TKDTFGREDY IGHV3-74 IGHD3-3 IGHJ4 ggattcaccttcagtagatactgg atgcactgggtccgccaggctccagggaaggggctggagtgggtcgcacgt actaatgaagatgggagtactaaa aactacgcggactccgtgaagggccgattcaccatcttcagagacaacaccaagaacacactatatctgcaaatgaacagtctgagagacgaggacacggctgtgtattattgt acaaaagacacgtttgggagagaagactac IGA2 18 213 11 7 8 12 3 6 4 0 6 8 JY8QFUQ01B3G3T 7339 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 976 94.91 205/216 nt 94.44 204/216 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD4-23*01 ORF 2 8 8 11 8.8.11 [1.17.37.11] CNLHPYGDSSDYW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 85 of V-REGION: 1 nucleotides (from position 160 in the user submitted sequence), (cause frameshift) ctctggactcaccttcagtcgcctctggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtatagatagtgatgggaataacataatctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaaacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtatattactgtaaccttcatccctacggtgactcctccgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [281] t82>c,F28>L(+ - -)|a106>c,S36>R(- - -)|t109>c,Y37>L(- - -)|a110>t,Y37>L(- - -)|t168>a|a169>g,N57>D(+ + -)|g188>a,S63>N(- - -)|g191>a,S64>N(- - -)|c194>t,T65>I(- - -)|g197>t,S66>I(- - -)|g303>a| t82>c,F28>L(+ - -)|a106>c,S36>R(- - -)|t109>c,Y37>L(- - -)|a110>t,Y37>L(- - -)| t168>a|a169>g,N57>D(+ + -)|g188>a,S63>N(- - -)|g191>a,S64>N(- - -)|c194>t,T65>I(- - -)| g197>t,S66>I(- - -)|g303>a| g313>a,A105>N(- - -)|c314>a,A105>N(- - -)|a315>c,A105>N(- - -)|a316>c,R106>L(- + -)|g317>t,R106>L(- + -)|a318>t,R106>L(- + -)|g319>c| 312 (320) 216 (224) 205 (206) 11 (18) 2 (3) 9 (15) 1 3 (4) 1 2 1 (3) 0 (1) 1 (2) 1 0 (1) 0 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 2 1 0 1 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 19 5 1 4 1 2 1 0 0 0 0 1 0 0 0 0 117 113 111 2 1 1 0 1 0 0 0 0 0 0 0 0 1 0 0 (8) 0 0 (1) 0 (7) 0 (1) 0 (6) 0 0 (1) 0 0 0 (2) 0 (1) 0 (1) 0 0 (1) 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggactcaccttcagtcgcctctgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt atagatagtgatgggaataacata atctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaaacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtatattactgt aaccttcatccctacggtgactcctccgactac NLHPYGDSSDY IGHV3-74 IGHD4-23 IGHJ4 ggactcaccttcagtcgcctctgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt atagatagtgatgggaataacata atctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaaacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtatattactgt aaccttcatccctacggtgactcctccgactac IGG2 11 212 7 4 4 5 3 6 1 1 1 8 JY8QFUQ01B3HMA 13838 productive (see comment) Homsap IGHV4-61*02 F 883 89.5 196/219 nt 87.67 192/219 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-19*01 F 1 10 7 13 10.7.13 [1.16.38.11] CARQGDSSGPWNDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 39 of V-REGION: 'G' (1 nucleotides at position 36 to 36 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 68 of V-REGION: 'A' (1 nucleotides at position 114 to 114 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 102 of V-REGION: 'C' (1 nucleotides at position 215 to 215 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 50 of V-REGION: 1 nucleotides (from position 70 in the user submitted sequence), (cause frameshift) ctccggtggctccatcagtactggttattactactGggagctggatccggcagtccgccgggaagggactgaatggattgggcgcatgtctgccagaggggacagcaactacaAacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtaCtttttgtgcgagacaaggggatagtagtggaccctggaatgactactggggccagggaatcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [293] t78>c|c93>t|g101>c,S34>T(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c133>t,P45>S(+ - -)|g153>a|t165>c|c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>g,T65>S(+ - +)|a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| t78>c| c93>t|g101>c,S34>T(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)| c133>t,P45>S(+ - -)|g153>a|t165>c| c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>g,T65>S(+ - +)| a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g319>c| 318 (320) 225 (227) 202 (203) 23 (24) 5 (6) 18 3 4 3 2 3 0 2 1 2 (3) 3 0 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 30 26 4 1 3 0 1 1 0 0 0 1 0 1 0 0 0 51 50 47 3 2 1 0 1 1 1 0 0 0 0 0 0 0 0 30 21 14 7 0 7 2 1 0 0 1 0 0 1 0 2 0 0 117 114 106 8 1 7 1 1 1 0 2 0 1 0 1 1 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctcc ggtggctccatcagtactggttattactac tggagctggatccggcagtccgccgggaagggactgaatggattgggcgc atgtctgccagaggggacagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac ARQGDSSGPWNDY IGHV4-61 IGHD6-19 IGHJ4 ggtggctccatcagtactggttattactac tggagctggatccggcagtccgccgggaagggactgaatggattgggcgc atgtctgccagaggggacagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac IGG2 22 215 11 11 7 12 4 10 3 1 8 10 JY8QFUQ01B3IOP 12218 productive Homsap IGHV3-72*01 F 894 89.24 199/223 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-19*01 F 1 8 10 12 8.10.12 [1.17.38.11] CARDLGNGWGYDYW in-frame + ctctggcttcatcttcagtgaccactatatggactgggtccgccaggctccagggaaggggctggagtgggttggccgtactagagttgcatctaacacttacgccacagaatacgccgcgtctttcaaaggcagattcgtcatctcaagagacaattcaaggaactcattgtttctccaaatgaacagcctgaaaaccgacgacacggccatgtattattgtgctagagatctgggcaatggctggggctatgactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [291, 290] a81>c|c86>t,T29>I(- - -)|c114>t|a172>g,N58>V(- - -)|a173>t,N58>V(- - -)|c174>t,N58>V(- - -)|a175>g,K59>A(- - -)|a176>c,K59>A(- - -)|g178>t,A60>S(- + -)|g185>c,S62>T(+ - +)|a190>g,T64>A(- - -)|g211>t,V71>F(+ - -)|g213>c,V71>F(+ - -)|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|t243>c|g244>a,D82>N(+ + -)|a251>g,K84>R(+ + +)|c259>t|a263>t,Y88>F(- + -)|g267>c|g291>c,E97>D(+ - +)|g301>a,V101>M(+ - -)|c309>t| a81>c|c86>t,T29>I(- - -)|c114>t| a172>g,N58>V(- - -)|a173>t,N58>V(- - -)|c174>t,N58>V(- - -)|a175>g,K59>A(- - -)|a176>c,K59>A(- - -)|g178>t,A60>S(- + -)|g185>c,S62>T(+ - +)|a190>g,T64>A(- - -)| g211>t,V71>F(+ - -)|g213>c,V71>F(+ - -)|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|t243>c|g244>a,D82>N(+ + -)|a251>g,K84>R(+ + +)|c259>t|a263>t,Y88>F(- + -)|g267>c|g291>c,E97>D(+ - +)|g301>a,V101>M(+ - -)|c309>t| 320 231 207 24 6 18 5 2 6 1 2 0 2 0 4 0 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 2 0 1 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 22 8 0 8 3 0 1 0 1 0 1 0 1 0 1 0 117 114 101 13 4 9 2 2 3 1 0 0 1 0 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,165-168(FR2-CDR2)|agct,177-180(CDR2)|aact,253-256(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tgct,312-315(FR3)| ctct ggcttcatcttcagtgaccactat atggactgggtccgccaggctccagggaaggggctggagtgggttggccgt actagagttgcatctaacacttacgccaca gaatacgccgcgtctttcaaaggcagattcgtcatctcaagagacaattcaaggaactcattgtttctccaaatgaacagcctgaaaaccgacgacacggccatgtattattgt gctagagatctgggcaatggctggggctatgactat ARDLGNGWGYDY IGHV3-72 IGHD6-19 IGHJ4 ggcttcatcttcagtgaccactat atggactgggtccgccaggctccagggaaggggctggagtgggttggccgt actagagttgcatctaacacttacgccaca gaatacgccgcgtctttcaaaggcagattcgtcatctcaagagacaattcaaggaactcattgtttctccaaatgaacagcctgaaaaccgacgacacggccatgtattattgt gctagagatctgggcaatggctggggctatgactat IGA1 24 219 14 10 8 14 6 10 4 2 9 9 JY8QFUQ01B3JLF 6599 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*02 F or Homsap IGHV4-39*05 F (see comment) 785 84.16 186/221 nt 83.71 185/221 nt Homsap IGHJ4*02 F (see comment) 204 91.67 44/48 nt Homsap IGHD3-3*01 F 3 10 7 16 10.7.16 [1.17.38.11] CARHRSFGVIIVNYFESW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (84,16% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) in CDR2-IMGT at codon 59 of V-REGION: 'C' (1 nucleotides at position 96 to 96 in the user submitted sequence), (cause frameshift); tttccggtgactccatcaccagtactaattattactggggctgggtccgccagtccccagggaagggtctggagtggattggaagtgtctattatCggggggacccagtacctcaacccgtctctccacaatcgagtcaccatatccattggcacgtccaagacccaattctccatgagactgacctctgtgaccgccgcagacacggctgtatatttctgtgcgagacatcggtcttttggagtgataattgttaactactttgagtcctggggacaggggaccctggtcaccgtctcctcagcttccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG3 100 100 [302] c75>t|t78>c|g83>a,G28>D(- - -)|g92>c,S31>T(+ - +)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c111>t|a124>g,I42>V(+ - +)|c133>t,P45>S(+ - -)|g147>t|g162>a|a166>g,I56>V(+ - +)|a175>g,S59>G(+ + -)|t177>g,S59>G(+ + -)|g191>c,S64>T(+ - +)|a193>c,T65>Q(- - -)|c194>a,T65>Q(- - -)|c195>g,T65>Q(- - -)|t199>c,Y67>L(- - -)|a200>t,Y67>L(- - -)|c210>t|a214>c,K72>H(- - +)|g216>c,K72>H(- - +)|g221>a,S74>N(- - -)|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|a242>g,D81>G(- - -)|a254>c,N85>T(- + -)|g258>a|c265>a,L89>M(+ + -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|g275>c,S92>T(+ - +)|g303>a|a308>t,Y103>F(- + -)| c75>t|t78>c| g83>a,G28>D(- - -)|g92>c,S31>T(+ - +)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c111>t| a124>g,I42>V(+ - +)|c133>t,P45>S(+ - -)|g147>t|g162>a| a166>g,I56>V(+ - +)|a175>g,S59>G(+ + -)|t177>g,S59>G(+ + -)|g191>c,S64>T(+ - +)|a193>c,T65>Q(- - -)|c194>a,T65>Q(- - -)|c195>g,T65>Q(- - -)| t199>c,Y67>L(- - -)|a200>t,Y67>L(- - -)|c210>t|a214>c,K72>H(- - +)|g216>c,K72>H(- - +)|g221>a,S74>N(- - -)|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|a242>g,D81>G(- - -)|a254>c,N85>T(- + -)|g258>a|c265>a,L89>M(+ + -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|g275>c,S92>T(+ - +)|g303>a|a308>t,Y103>F(- + -)| 320 229 194 35 8 27 5 8 4 2 3 2 3 0 5 1 1 1 78 5 3 2 2 0 0 0 1 1 0 0 0 0 0 0 0 0 36 30 25 5 1 4 0 2 1 0 0 0 0 0 2 0 0 0 51 51 47 4 2 2 1 1 1 0 0 0 0 0 0 0 1 0 30 21 14 7 0 7 2 0 0 0 1 1 0 0 1 1 0 1 117 114 97 17 3 14 2 5 1 1 2 1 3 0 2 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tttcc ggtgactccatcaccagtactaattattac tggggctgggtccgccagtccccagggaagggtctggagtggattggaagt gtctattatggggggacccag tacctcaacccgtctctccacaatcgagtcaccatatccattggcacgtccaagacccaattctccatgagactgacctctgtgaccgccgcagacacggctgtatatttctgt gcgagacatcggtcttttggagtgataattgttaactactttgagtcc ARHRSFGVIIVNYFES IGHV4-39 IGHD3-3 IGHJ4 ggtgactccatcaccagtactaattattac tggggctgggtccgccagtccccagggaagggtctggagtggattggaagt gtctattatggggggacccag tacctcaacccgtctctccacaatcgagtcaccatatccattggcacgtccaagacccaattctccatgagactgacctctgtgaccgccgcagacacggctgtatatttctgt gcgagacatcggtcttttggagtgataattgttaactactttgagtcc IGG3 33 216 17 16 11 20 6 13 5 1 16 11 JY8QFUQ01B3L4U 7923 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 954 93.55 203/217 nt 93.09 202/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVRAGFGELSGFDCW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 50 of V-REGION: 'G' (1 nucleotides at position 63 to 63 in the user submitted sequence), (cause frameshift); ctctggattcacctttggttattatggcatgagctgggtccgccaagctccgggggaggggcGtggagtgggtctctggtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [289] a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a138>g|a142>g,K48>E(+ - -)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a138>g|a142>g,K48>E(+ - -)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 224 (225) 209 15 (16) 3 (4) 12 3 3 1 1 1 (2) 0 2 0 2 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 51 49 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ctct ggattcacctttggttattatggc atgagctgggtccgccaagctccgggggaggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgagctgggtccgccaagctccgggggaggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 14 213 7 7 3 7 4 7 3 0 7 4 JY8QFUQ01B3OBX 14025 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 930 91.03 203/223 nt NA Homsap IGHJ4*03 F (see comment) 78 62.5 30/48 nt Homsap IGHD3-22*01 F 3 8 10 24 8.10.24 [1.17.38.11] CATDRPYYKKSLLADSGITTVWTFWG in-frame + Other possibilities: Homsap_IGHJ1*01 (shorter alignment but highest percentage of identity) ctctggattcactttcactaacgcgtggatgagttgggtccgccaggctccagggaaggggctggagtgggttgcccgtgttaaaactaagactgacgatggggcaacagactacgctgcacccgtgaaaggcagattccttatctcaagagatgattcaaacaacatactgtatctgcaaatgaacagcctgagaaccgaggacacagccatgtactactgtgccacagatcggccttactacaaaaaatcgctgctggccgactctggtattactacggtatggacgttttgggggcccaaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 79 88 [327, 328] g104>c,S35>T(+ - +)|c111>g|c120>t|g161>c,G54>A(- + -)|a166>g,I56>V(+ - +)|g173>c,S58>T(+ - +)|c174>t,S58>T(+ - +)|a177>g|t183>c|g185>a,G62>D(- - -)|a190>g,T64>A(- - -)|a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|c231>t,T77>L(- - -)|a252>c,K84>N(+ - -)|c257>t,T86>I(- - -)|g258>a,T86>I(- - -)|a284>g,K95>R(+ + +)|g301>a,V101>M(+ - -)|t306>c|a313>g,T105>A(- - -)| g104>c,S35>T(+ - +)|c111>g| c120>t|g161>c,G54>A(- + -)| a166>g,I56>V(+ - +)|g173>c,S58>T(+ - +)|c174>t,S58>T(+ - +)|a177>g|t183>c|g185>a,G62>D(- - -)|a190>g,T64>A(- - -)| a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|c231>t,T77>L(- - -)|a252>c,K84>N(+ - -)|c257>t,T86>I(- - -)|g258>a,T86>I(- - -)|a284>g,K95>R(+ + +)|g301>a,V101>M(+ - -)|t306>c| a313>g,T105>A(- - -)| 320 231 210 21 5 16 5 3 5 2 2 0 0 0 3 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 0 0 0 0 0 0 1 1 0 0 51 51 49 2 1 1 0 0 1 0 0 0 0 0 1 0 0 0 30 30 23 7 2 5 3 1 1 1 0 0 0 0 1 0 0 0 117 114 105 9 1 8 1 2 3 1 2 0 0 0 0 0 0 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| ctct ggattcactttcactaacgcgtgg atgagttgggtccgccaggctccagggaaggggctggagtgggttgcccgt gttaaaactaagactgacgatggggcaaca gactacgctgcacccgtgaaaggcagattccttatctcaagagatgattcaaacaacatactgtatctgcaaatgaacagcctgagaaccgaggacacagccatgtactactgt gccacagatcggccttactacaaaaaatcgctgctggccgactctggtattactacggtatggacgttttgg ATDRPYYKKSLLADSGITTVWTFW IGHV3-15 IGHD3-22 IGHJ4 ggattcactttcactaacgcgtgg atgagttgggtccgccaggctccagggaaggggctggagtgggttgcccgt gttaaaactaagactgacgatggggcaaca gactacgctgcacccgtgaaaggcagattccttatctcaagagatgattcaaacaacatactgtatctgcaaatgaacagcctgagaaccgaggacacagccatgtactactgt gccacagatcggccttactacaaaaaatcgctgctggccgactctggtattactacggtatggacgttttgg IGG1 20 219 14 6 8 12 6 8 2 3 9 6 JY8QFUQ01B3P0G 12271 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 850 88.43 191/216 nt 87.96 190/216 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-15*01 F 3 8 8 14 8.8.14 [1.16.38.11] CAKEEGATGVYYVDHW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 45 of V-REGION: 1 nucleotides (from position 48 in the user submitted sequence), (cause frameshift) ctctggattcacctttaccacctccgccatggcctgggtccgccaggtccagggaaggggctggagtgggtctcaactattagacctagtagtgagagaacctactacgcagagtccgtgaggggccgcttcaccatctccagagacaattccgagaacacgttgtatctacaactgaacaacctgagagtcgaggacacggccatatattactgtgcgaaggaggaaggtgctactggagtctattatgttgaccactggggccagggaacccgggtcaccgtctcctcatcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [290] g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g163>a,A55>T(- - -)|t171>a,S57>R(- - -)|g172>c,G58>P(+ - -)|g173>c,G58>P(+ - -)|g184>a,G62>S(+ + -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)|a195>c|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|g225>c|a250>g,K84>E(+ - -)|c259>t|g267>a|a271>c,M91>L(+ + -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|a318>g| g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g163>a,A55>T(- - -)| t171>a,S57>R(- - -)|g172>c,G58>P(+ - -)|g173>c,G58>P(+ - -)|g184>a,G62>S(+ + -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)|a195>c| c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|g225>c|a250>g,K84>E(+ - -)|c259>t|g267>a|a271>c,M91>L(+ + -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)| a318>g| 320 224 198 26 5 21 4 6 2 1 3 1 0 1 6 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 1 1 0 0 0 2 0 0 0 51 50 47 3 0 3 1 1 0 0 0 0 0 0 1 0 0 0 30 24 16 8 1 7 0 2 0 0 1 1 0 1 2 0 0 1 117 114 104 10 3 7 2 3 2 0 1 0 0 0 1 1 0 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaccacctccgcc atggcctgggtccgccaggtccagggaaggggctggagtgggtctcaact attagacctagtagtgagagaacc tactacgcagagtccgtgaggggccgcttcaccatctccagagacaattccgagaacacgttgtatctacaactgaacaacctgagagtcgaggacacggccatatattactgt gcgaaggaggaaggtgctactggagtctattatgttgaccac AKEEGATGVYYVDH IGHV3-23 IGHD2-15 IGHJ4 ggattcacctttaccacctccgcc atggcctgggtccgccaggtccagggaaggggctggagtgggtctcaact attagacctagtagtgagagaacc tactacgcagagtccgtgaggggccgcttcaccatctccagagacaattccgagaacacgttgtatctacaactgaacaacctgagagtcgaggacacggccatatattactgt gcgaaggaggaaggtgctactggagtctattatgttgaccac IGG2 25 212 12 13 8 16 4 9 3 1 10 11 JY8QFUQ01B3S4F 16008 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 991 94.98 208/219 nt 94.06 206/219 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-8*01 F 2 8 8 8 8.8.8 [2.17.38.11] CARDNYGDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 35 of V-REGION: 'G' (1 nucleotides at position 21 to 21 in the user submitted sequence), (cause frameshift);in CDR1-IMGT at codon 38 of V-REGION: 'G' (1 nucleotides at position 30 to 30 in the user submitted sequence), (cause frameshift); gcctctggattcactttcagGtagctactGggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtgttaatggtgatgggggtaggacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcacagtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [276] c87>t|a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| c87>t| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| 320 227 216 11 5 6 4 1 1 0 0 0 0 0 3 1 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 3 0 0 0 0 0 0 0 0 1 0 0 117 114 108 6 4 2 1 1 0 0 0 0 0 0 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcactttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcactttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac IGA2 11 213 6 5 2 7 4 4 4 1 2 4 JY8QFUQ01B3TI8 17861 productive (see comment) Homsap IGHV3-7*01 F 885 90.65 194/214 nt 90.19 193/214 nt Homsap IGHJ5*02 F (see comment) 192 86.27 44/51 nt Homsap IGHD4-23*01 ORF 3 8 8 12 8.8.12 [0.17.38.11] CARGTSVDPGIDHW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 48 of V-REGION: 'G' (1 nucleotides at position 54 to 54 in the user submitted sequence), (cause frameshift); tggattcacctttagtaattattggatgcactgggtccgccaggtttcagggaGgggggctggagtgcgtggccaccataaacgaagatggaagtgagagacactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactctctgtttttgcagatgaacagcctgagagccgaggacacggctgtctactactgtgcgagagggacctcagtggacccggggattgaccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [282] g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)|c134>t,A45>V(+ - +)|c136>t,P46>S(+ - -)|a143>g,K48>R(+ + +)|g156>c,W52>C(+ - -)|a164>c,N55>T(- + -)|g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)|t196>c,Y66>H(+ - -)|a232>g,I78>V(+ - +)|a258>t|a263>t,Y88>F(- + -)|c265>t|a270>g|g303>c|t306>c| g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)|c134>t,A45>V(+ - +)|c136>t,P46>S(+ - -)|a143>g,K48>R(+ + +)|g156>c,W52>C(+ - -)|a164>c,N55>T(- + -)| g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)| t196>c,Y66>H(+ - -)|a232>g,I78>V(+ - +)|a258>t|a263>t,Y88>F(- + -)|c265>t|a270>g|g303>c|t306>c| a320>g| 319 (320) 221 (222) 201 20 (21) 5 (6) 15 4 (5) 2 4 2 2 0 2 0 3 1 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 51 51 44 7 0 7 1 1 2 0 2 0 0 0 1 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 1 1 0 0 117 114 106 8 5 3 2 0 1 2 0 0 2 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t ggattcacctttagtaattattgg atgcactgggtccgccaggtttcagggagggggctggagtgcgtggccacc ataaacgaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactctctgtttttgcagatgaacagcctgagagccgaggacacggctgtctactactgt gcgagagggacctcagtggacccggggattgaccac ARGTSVDPGIDH IGHV3-7 IGHD4-23 IGHJ5 ggattcacctttagtaattattgg atgcactgggtccgccaggtttcagggagggggctggagtgcgtggccacc ataaacgaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactctctgtttttgcagatgaacagcctgagagccgaggacacggctgtctactactgt gcgagagggacctcagtggacccggggattgaccac IGA1 20 213 12 8 6 10 6 10 5 0 10 5 JY8QFUQ01B3TT1 20530 productive Homsap IGHV1-2*02 F (see comment) 777 84.58 181/214 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-22*01 F 2 8 8 14 8.8.14 [0.17.38.11] CARGLSYDSSPYWGYW in-frame + Low V-REGION identity (84,58% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tggatacatcttcaatgacttctacatatattgggtgcgacaggcccctggacaagggcttgagtggatgggatggttcaaccctaatagtcgtgtcacagactatgcagagaaatttcagggcagggtcaccatgaccggggacccgtcccgcagcacagtccacctgaaactgacccgcctgaagtcggacgacacggccgtctattactgcgcgagaggtctctcctatgacagtagtccttattggggctactggggccagggaaccgtggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [287] c86>t,T29>I(- - -)|c104>a,T35>N(- + -)|c105>t,T35>N(- + -)|g107>a,G36>D(- - -)|a110>t,Y37>F(- + -)|t114>c|g117>a,M39>I(+ + -)|c118>t,H40>Y(+ - -)|c120>t,H40>Y(+ - -)|a166>t,I56>F(+ - -)|c177>t|g187>c,G63>R(- - -)|g191>t,G64>V(- - -)|a196>g,N66>D(+ + -)|c205>g,Q69>E(+ + -)|g210>a|a238>g,R80>G(- - -)|a244>c,T82>P(+ + -)|a250>c,I84>R(- + -)|t251>g,I84>R(- + -)|c260>t,A87>V(+ - +)|t262>c,Y88>H(+ - -)|a265>c,M89>L(+ + -)|g268>a,E90>K(+ - -)|g270>a,E90>K(+ - -)|g275>c,S92>T(+ - +)|a277>c|g279>c|g284>a,R95>K(+ + +)|a285>g,R95>K(+ + +)|t288>g|g303>c|t312>c| c86>t,T29>I(- - -)|c104>a,T35>N(- + -)|c105>t,T35>N(- + -)|g107>a,G36>D(- - -)|a110>t,Y37>F(- + -)|t114>c| g117>a,M39>I(+ + -)|c118>t,H40>Y(+ - -)|c120>t,H40>Y(+ - -)| a166>t,I56>F(+ - -)|c177>t|g187>c,G63>R(- - -)|g191>t,G64>V(- - -)| a196>g,N66>D(+ + -)|c205>g,Q69>E(+ + -)|g210>a|a238>g,R80>G(- - -)|a244>c,T82>P(+ + -)|a250>c,I84>R(- + -)|t251>g,I84>R(- + -)|c260>t,A87>V(+ - +)|t262>c,Y88>H(+ - -)|a265>c,M89>L(+ + -)|g268>a,E90>K(+ - -)|g270>a,E90>K(+ - -)|g275>c,S92>T(+ - +)|a277>c|g279>c|g284>a,R95>K(+ + +)|a285>g,R95>K(+ + +)|t288>g|g303>c|t312>c| a320>g| 319 (320) 221 (222) 188 33 (34) 8 (9) 25 3 (4) 6 6 3 4 1 2 0 4 1 1 2 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 1 5 0 1 2 1 0 1 1 0 0 0 0 0 51 51 48 3 0 3 0 1 2 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 0 0 1 0 0 0 1 0 1 0 1 0 117 114 94 20 6 14 3 4 1 2 4 0 0 0 3 1 0 2 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatacatcttcaatgacttctac atatattgggtgcgacaggcccctggacaagggcttgagtggatgggatgg ttcaaccctaatagtcgtgtcaca gactatgcagagaaatttcagggcagggtcaccatgaccggggacccgtcccgcagcacagtccacctgaaactgacccgcctgaagtcggacgacacggccgtctattactgc gcgagaggtctctcctatgacagtagtccttattggggctac ARGLSYDSSPYWGY IGHV1-2 IGHD3-22 IGHJ4 ggatacatcttcaatgacttctac atatattgggtgcgacaggcccctggacaagggcttgagtggatgggatgg ttcaaccctaatagtcgtgtcaca gactatgcagagaaatttcagggcagggtcaccatgaccggggacccgtcccgcagcacagtccacctgaaactgacccgcctgaagtcggacgacacggccgtctattactgc gcgagaggtctctcctatgacagtagtccttattggggctac IGA1 33 213 18 15 12 19 6 14 6 2 17 8 JY8QFUQ01B3XMD 14821 productive (see comment) Homsap IGHV3-7*01 F 837 87.56 190/217 nt 87.1 189/217 nt Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 147 76.47 39/51 nt Homsap IGHD5-12*01 F 3 8 8 13 8.8.13 [1.17.38.11] CGRDERGADLPNDSW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 and Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR3-IMGT at codon 69 of V-REGION: 'G' (1 nucleotides at position 114 to 114 in the user submitted sequence), (cause frameshift); ctctggattcacctttagtaggtattggatgagctgggtccgccagtctccagggaagggactggagtggctggcccacataggaggagatggaagtgaggctggttatgtggGactctgtgaggggccgattcttcatctccagagacaacgccaagagctccctctatctgcagatgaacagcctgagccccgaggacacgggtgtgtattattgtggcagagatgaaaggggcgccgacttgccgaatgattcctggggcctgggaaccctggtcaccgtctcctcaccatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [288] c108>g,S36>R(- - -)|g133>t,A45>S(- + -)|g147>a|g157>c,V53>L(+ - +)|a163>c,N55>H(- - -)|a169>g,K57>G(- - -)|a170>g,K57>G(- - -)|g171>a,K57>G(- - -)|c172>g,Q58>G(- - -)|a173>g,Q58>G(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)|a195>t,K65>A(- - -)|t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>t,Y66>G(+ - -)|a215>g,K72>R(+ + +)|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|a254>g,N85>S(- - -)|a258>c|g261>c|a270>g|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|c299>g,A100>G(- + -)|c309>t|c314>g,A105>G(- + -)|g315>c,A105>G(- + -)| c108>g,S36>R(- - -)| g133>t,A45>S(- + -)|g147>a|g157>c,V53>L(+ - +)|a163>c,N55>H(- - -)| a169>g,K57>G(- - -)|a170>g,K57>G(- - -)|g171>a,K57>G(- - -)|c172>g,Q58>G(- - -)|a173>g,Q58>G(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)|a195>t,K65>A(- - -)| t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>t,Y66>G(+ - -)|a215>g,K72>R(+ + +)|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|a254>g,N85>S(- - -)|a258>c|g261>c|a270>g|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|c299>g,A100>G(- + -)|c309>t| c314>g,A105>G(- + -)|g315>c,A105>G(- + -)| 320 225 196 29 5 24 8 2 3 0 4 0 2 0 4 4 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 47 4 1 3 0 1 0 0 1 0 0 0 1 0 1 0 30 24 16 8 0 8 4 1 0 0 1 0 1 0 0 1 0 0 117 114 100 14 4 10 4 0 3 0 2 0 1 0 2 1 0 1 8 8 6 2 0 2 0 0 0 0 0 0 0 0 1 1 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtaggtattgg atgagctgggtccgccagtctccagggaagggactggagtggctggcccac ataggaggagatggaagtgaggct ggttatgtggactctgtgaggggccgattcttcatctccagagacaacgccaagagctccctctatctgcagatgaacagcctgagccccgaggacacgggtgtgtattattgt ggcagagatgaaaggggcgccgacttgccgaatgattcc GRDERGADLPNDS IGHV3-7 IGHD5-12 IGHJ5 ggattcacctttagtaggtattgg atgagctgggtccgccagtctccagggaagggactggagtggctggcccac ataggaggagatggaagtgaggct ggttatgtggactctgtgaggggccgattcttcatctccagagacaacgccaagagctccctctatctgcagatgaacagcctgagccccgaggacacgggtgtgtattattgt ggcagagatgaaaggggcgccgacttgccgaatgattcc IGA2 27 213 13 14 5 12 8 15 5 0 13 9 JY8QFUQ01B4CN9 17949 productive Homsap IGHV4-61*02 F 902 90.05 199/221 nt NA Homsap IGHJ5*02 F 228 94.12 48/51 nt Homsap IGHD3-22*01 F 3 10 7 14 10.7.14 [1.17.38.11] CAREKVVIRPNWFDPW in-frame + tctctggtgactccatcaccagtggtagttattattggagttggatccggcagcccgccgagaagggactggagtggattgggcgtatctccatcggtgggagcaccaactacaatccctccctcaagagtcgagtcaccatagtattagacacgatcaacaaccggttctccctgcagctcaggtctgtgaccgccgcagacacggccgtatattactgtgcgagagaaaaagtagtgatccgtcccaactggttcgacccctggggccagggagccctggtcatcgtctcctcagcctccaccaagggcccatcggttttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 88 [295] g83>a,G28>D(- - -)|g92>c,S31>T(+ - +)|c111>t|c114>t|c120>t|g140>a,G47>E(- - -)|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|c173>t,T58>I(- - -)|a175>g,S59>G(+ + -)|c204>t|t235>g,S79>V(- - -)|c236>t,S79>V(- - -)|g238>t,V80>L(+ - +)|t247>a,S83>I(- - -)|c248>t,S83>I(- - -)|g252>c,K84>N(+ - -)|a257>g,Q86>R(+ - -)|a268>c,K90>Q(+ - -)|g273>c|c276>g,S92>R(- - -)|g303>a| g83>a,G28>D(- - -)|g92>c,S31>T(+ - +)|c111>t|c114>t| c120>t|g140>a,G47>E(- - -)| a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|c173>t,T58>I(- - -)|a175>g,S59>G(+ + -)| c204>t|t235>g,S79>V(- - -)|c236>t,S79>V(- - -)|g238>t,V80>L(+ - +)|t247>a,S83>I(- - -)|c248>t,S83>I(- - -)|g252>c,K84>N(+ - -)|a257>g,Q86>R(+ - -)|a268>c,K90>Q(+ - -)|g273>c|c276>g,S92>R(- - -)|g303>a| 320 229 207 22 6 16 2 3 7 1 2 0 0 1 3 1 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 2 2 0 1 2 0 0 0 0 0 1 0 0 0 51 51 49 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 30 21 17 4 0 4 1 0 1 1 1 0 0 0 0 0 0 0 117 114 102 12 3 9 1 1 3 0 1 0 0 1 2 1 1 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatcaccagtggtagttattat tggagttggatccggcagcccgccgagaagggactggagtggattgggcgt atctccatcggtgggagcacc aactacaatccctccctcaagagtcgagtcaccatagtattagacacgatcaacaaccggttctccctgcagctcaggtctgtgaccgccgcagacacggccgtatattactgt gcgagagaaaaagtagtgatccgtcccaactggttcgacccc AREKVVIRPNWFDP IGHV4-61 IGHD3-22 IGHJ5 ggtgactccatcaccagtggtagttattat tggagttggatccggcagcccgccgagaagggactggagtggattgggcgt atctccatcggtgggagcacc aactacaatccctccctcaagagtcgagtcaccatagtattagacacgatcaacaaccggttctccctgcagctcaggtctgtgaccgccgcagacacggccgtatattactgt gcgagagaaaaagtagtgatccgtcccaactggttcgacccc IGG1 22 216 13 9 10 15 3 7 4 2 10 6 JY8QFUQ01B4F4Q 14873 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 806 85.78 187/218 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-15*01 F 2 8 8 19 8.8.19 [1.17.38.11] CVKDYGNCGGGRCYSIPLDYW in-frame + cctctggatttacctttagccgctacgccatgagctgggtccgccaggctccggggaaggggctggagtgggtctcaggcattattgatgttaattctttcacatactacgcagactctgttcggggccggttcaccgtgtccagagacgattccaagaacacggtatatctgcaaatgaacagtttgagacccgacgacacggccgtttattattgtgtgaaagattacggtaactgtggtggtggtcgctgctattcgattccccttgactactggggccggggaaccgtggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [306] c84>t|a106>c,S36>R(- - -)|t111>c|a138>g|c164>g,A55>G(- + -)|t165>c,A55>G(- + -)|g170>t,S57>I(- - -)|g173>a,G58>D(- - -)|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|g184>a,G62>N(- - -)|g185>a,G62>N(- - -)|g187>t,G63>S(+ + -)|g188>c,G63>S(+ + -)|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)|c210>t|g213>t|a214>c,K72>R(+ + +)|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|c234>g,I78>V(+ - +)|a244>g,N82>D(+ + -)|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|c280>t|g286>c,A96>P(- - -)|g291>c,E97>D(+ - +)|a303>t|c309>t|c314>t,A105>V(+ - +)| c84>t|a106>c,S36>R(- - -)|t111>c| a138>g|c164>g,A55>G(- + -)|t165>c,A55>G(- + -)| g170>t,S57>I(- - -)|g173>a,G58>D(- - -)|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|g184>a,G62>N(- - -)|g185>a,G62>N(- - -)|g187>t,G63>S(+ + -)|g188>c,G63>S(+ + -)|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)| c210>t|g213>t|a214>c,K72>R(+ + +)|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|c234>g,I78>V(+ - +)|a244>g,N82>D(+ + -)|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|c280>t|g286>c,A96>P(- - -)|g291>c,E97>D(+ - +)|a303>t|c309>t| c314>t,A105>V(+ - +)| 320 226 194 32 9 23 5 4 6 2 2 0 2 0 3 3 5 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 1 1 1 0 0 0 0 0 0 0 51 51 48 3 1 2 1 0 0 1 0 0 0 0 0 1 0 0 30 24 14 10 0 10 1 3 0 0 0 0 1 0 1 0 4 0 117 114 99 15 6 9 3 1 4 0 1 0 1 0 2 2 1 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggatttacctttagccgctacgcc atgagctgggtccgccaggctccggggaaggggctggagtgggtctcaggc attattgatgttaattctttcaca tactacgcagactctgttcggggccggttcaccgtgtccagagacgattccaagaacacggtatatctgcaaatgaacagtttgagacccgacgacacggccgtttattattgt gtgaaagattacggtaactgtggtggtggtcgctgctattcgattccccttgactac VKDYGNCGGGRCYSIPLDY IGHV3-23 IGHD2-15 IGHJ4 ggatttacctttagccgctacgcc atgagctgggtccgccaggctccggggaaggggctggagtgggtctcaggc attattgatgttaattctttcaca tactacgcagactctgttcggggccggttcaccgtgtccagagacgattccaagaacacggtatatctgcaaatgaacagtttgagacccgacgacacggccgtttattattgt gtgaaagattacggtaactgtggtggtggtcgctgctattcgattccccttgactac IGA1 31 213 16 15 9 20 7 11 7 2 11 11 JY8QFUQ01B4FE6 12104 productive Homsap IGHV3-7*01 F 972 94.47 205/217 nt NA Homsap IGHJ6*02 F 238 87.1 54/62 nt Homsap IGHD3-22*01 F 2 8 8 14 8.8.14 [1.17.38.11] CAKDGYYYYHSGMDVW in-frame + ctctggattcagatttagtaactactggatgagttgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagcaagatggaggtgagaagtattatgtgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattattgtgcgaaagacggctattattattatcacagcggtatggacgtctggggccaagggaccacggtcatcgtctccccagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggctccagggcttcttc NA IGA2 100 100 [290] c86>g,T29>R(- - -)|c87>a,T29>R(- - -)|g107>a,S36>N(- - -)|t111>c|c120>t|a187>g,S63>G(+ + -)|a195>g|c198>t|a206>g,D69>G(- - -)|c259>g,L87>V(+ - +)|g303>a|c309>t|g317>a,R106>K(+ + +)| c86>g,T29>R(- - -)|c87>a,T29>R(- - -)|g107>a,S36>N(- - -)|t111>c| c120>t| a187>g,S63>G(+ + -)|a195>g| c198>t|a206>g,D69>G(- - -)|c259>g,L87>V(+ - +)|g303>a|c309>t| g317>a,R106>K(+ + +)| 320 225 212 13 6 7 3 3 3 1 0 1 0 0 0 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 0 1 0 1 0 0 0 1 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 22 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 117 114 109 5 3 2 1 1 2 0 0 0 0 0 0 1 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagatttagtaactactgg atgagttgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaggtgagaag tattatgtgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattattgt gcgaaagacggctattattattatcacagcggtatggacgtc AKDGYYYYHSGMDV IGHV3-7 IGHD3-22 IGHJ6 ggattcagatttagtaactactgg atgagttgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaggtgagaag tattatgtgggctctgtgaagggccgattcaccatctccagagacaacgccaagaactcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattattgt gcgaaagacggctattattattatcacagcggtatggacgtc IGA2 12 213 9 3 5 8 4 4 4 2 2 4 JY8QFUQ01B4FME 21001 productive Homsap IGHV3-7*01 F 968 94.04 205/218 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD3-22*01 F 2 8 8 22 8.8.22 [1.17.38.11] CVRDRFYDSSGYEFGELGDYSDHW in-frame + cctctggattcacatttagtaactactggatgagctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagaaagatggaagtgagaagtactatgtggactctgtgaagggccgattcaccatctccagagacgacgccaagaatacattgtatctgcaaatgaacagcctgagggccgaagacacggctgtatatttctgtgtgagagatcgattttatgatagtagtggttatgagtttggggaattgggggactactctgaccactggggccagggaacccaggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcaccctccgagagcacagccgccctgggctgcctggtcaaggactaccttccccggaaccggtgacggtgtcgtggaactcca NA IGG4 93 81 [316, 317] c87>a|g107>a,S36>N(- - -)|t111>c|c172>a,Q58>K(+ - -)|a195>g|a244>g,N82>D(+ + -)|c255>t|t256>a,S86>T(+ - +)|c259>t|a285>g|g291>a|g303>a|a308>t,Y103>F(- + -)|c314>t,A105>V(+ - +)| c87>a|g107>a,S36>N(- - -)|t111>c| c172>a,Q58>K(+ - -)|a195>g| a244>g,N82>D(+ + -)|c255>t|t256>a,S86>T(+ - +)|c259>t|a285>g|g291>a|g303>a|a308>t,Y103>F(- + -)| c314>t,A105>V(+ - +)| 320 226 212 14 8 6 3 3 3 1 0 2 1 1 0 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 1 0 1 0 1 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 1 1 1 0 0 0 0 1 0 0 0 0 0 0 117 114 106 8 5 3 2 2 2 0 0 0 1 1 0 0 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacatttagtaactactgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagaaagatggaagtgagaag tactatgtggactctgtgaagggccgattcaccatctccagagacgacgccaagaatacattgtatctgcaaatgaacagcctgagggccgaagacacggctgtatatttctgt gtgagagatcgattttatgatagtagtggttatgagtttggggaattgggggactactctgaccac VRDRFYDSSGYEFGELGDYSDH IGHV3-7 IGHD3-22 IGHJ4 ggattcacatttagtaactactgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagaaagatggaagtgagaag tactatgtggactctgtgaagggccgattcaccatctccagagacgacgccaagaatacattgtatctgcaaatgaacagcctgagggccgaagacacggctgtatatttctgt gtgagagatcgattttatgatagtagtggttatgagtttggggaattgggggactactctgaccac IGG4 13 213 9 4 5 7 4 6 5 3 3 2 JY8QFUQ01B4L0R 5949 productive Homsap IGHV3-48*01 F, or Homsap IGHV3-48*02 F 914 91.28 199/218 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD3-10*01 F 2 8 8 14 8.8.14 [1.17.38.11] CTRQTGNYYYFGVDVW in-frame - cctctggattcctcttcagtagctttaacatgaactgggtccgccacgttccagggaagggtctggagtgggtttcatacattaatagtagaggtactaacatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaggaattcactgtatctgcaaatgaacagcctgagagtcgacgacacggccgtatactactgtacgagacaaactgggaattattattacttcggtgtggacgtctggggccaagggactacggtcaccgtctcctctgcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [291] a85>c,T29>L(- - -)|c86>t,T29>L(- - -)|a110>t,Y37>F(- + -)|g113>a,S38>N(- - -)|g132>c,Q44>H(- + -)|c134>t,A45>V(+ - +)|g147>t|g170>a,S57>N(- - -)|t177>a,S59>R(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|c191>a,T64>N(- + -)|a251>g,K84>R(+ + +)|c255>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t300>c|g303>a|t306>c|g313>a,A105>T(- - -)| a85>c,T29>L(- - -)|c86>t,T29>L(- - -)|a110>t,Y37>F(- + -)|g113>a,S38>N(- - -)| g132>c,Q44>H(- + -)|c134>t,A45>V(+ - +)|g147>t| g170>a,S57>N(- - -)|t177>a,S59>R(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|c191>a,T64>N(- + -)| a251>g,K84>R(+ + +)|c255>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t300>c|g303>a|t306>c| g313>a,A105>T(- - -)|g319>c| 318 (320) 224 (226) 204 (205) 20 (21) 5 (6) 15 2 4 4 2 1 1 1 1 3 (4) 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 1 0 1 0 1 0 0 0 0 0 51 51 48 3 1 2 0 0 1 0 0 0 0 0 1 0 1 0 30 24 19 5 0 5 1 1 0 0 0 1 0 1 1 0 0 0 117 114 107 7 4 3 1 1 2 2 0 0 0 0 1 0 0 0 6 (8) 6 5 (6) 1 (2) 0 (1) 1 0 1 0 0 0 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcctcttcagtagctttaac atgaactgggtccgccacgttccagggaagggtctggagtgggtttcatac attaatagtagaggtactaacata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaggaattcactgtatctgcaaatgaacagcctgagagtcgacgacacggccgtatactactgt acgagacaaactgggaattattattacttcggtgtggacgtc TRQTGNYYYFGVDV IGHV3-48 IGHD3-10 IGHJ6 ggattcctcttcagtagctttaac atgaactgggtccgccacgttccagggaagggtctggagtgggtttcatac attaatagtagaggtactaacata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaggaattcactgtatctgcaaatgaacagcctgagagtcgacgacacggccgtatactactgt acgagacaaactgggaattattattacttcggtgtggacgtc IGA1 19 213 11 8 7 12 4 7 5 0 5 9 JY8QFUQ01B4VTT 10560 productive Homsap IGHV3-7*01 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD5-18*01 F 3 8 8 15 8.8.15 [1.17.38.11] CARMGYGYRGIYFFDYW in-frame + ctctacattcacgtttagtcggtattggatgagctgggtccgccaggctccagggaagggcctggagtgggtggccaacataaaggaagacggaagtgagagatattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgtgcgagaatgggctatggttaccgaggaatatacttctttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [294] g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|c87>g|a106>c,S36>R(- - -)|c108>g,S36>R(- - -)|g147>c|c172>g,Q58>E(+ + -)|t177>c|a194>g,K65>R(+ + +)|c198>t|c246>t|a258>t|a271>t,M91>L(+ + -)|c280>t| g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|c87>g|a106>c,S36>R(- - -)|c108>g,S36>R(- - -)| g147>c| c172>g,Q58>E(+ + -)|t177>c|a194>g,K65>R(+ + +)| c198>t|c246>t|a258>t|a271>t,M91>L(+ + -)|c280>t| g319>a|a320>t| 318 (320) 223 (225) 209 14 (16) 7 (9) 7 1 1 (2) 3 1 1 0 2 (3) 0 2 3 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 0 0 1 0 0 0 1 2 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 21 3 1 2 1 0 0 1 0 0 0 0 0 1 0 0 117 114 109 5 4 1 0 0 3 0 0 0 2 0 0 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct acattcacgtttagtcggtattgg atgagctgggtccgccaggctccagggaagggcctggagtgggtggccaac ataaaggaagacggaagtgagaga tattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgt gcgagaatgggctatggttaccgaggaatatacttctttgactac ARMGYGYRGIYFFDY IGHV3-7 IGHD5-18 IGHJ4 acattcacgtttagtcggtattgg atgagctgggtccgccaggctccagggaagggcctggagtgggtggccaac ataaaggaagacggaagtgagaga tattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgt gcgagaatgggctatggttaccgaggaatatacttctttgactac IGG2 14 213 6 8 4 9 2 5 5 2 1 6 JY8QFUQ01B4X7N 9520 productive Homsap IGHV3-48*01 F 1080 100 217/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-2*01 F 2 8 8 10 8.8.10 [1.17.38.11] CASRLRSSGGYW in-frame + ctctggattcaccttcagtagctatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagtagtagtagtagtaccatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagccgcctacgtagttccggtgggtactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [279] a318>c,R106>S(- - -)|g319>c|a320>g| 317 (320) 222 (225) 222 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtagtagtagtagtaccata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagccgcctacgtagttccggtgggtac ASRLRSSGGY IGHV3-48 IGHD2-2 IGHJ4 ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtagtagtagtagtaccata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagccgcctacgtagttccggtgggtac IGG2 0 213 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01B4XPK 21346 productive (see comment) Homsap IGHV2-5*02 F 1165 97.94 238/243 nt 97.53 237/243 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD5-12*01 F 2 10 7 11 10.7.11 [9.17.38.11] CAHRRIRFGFDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 58 of V-REGION: 'G' (1 nucleotides at position 116 to 116 in the user submitted sequence), (cause frameshift); accctcacgctgacctgcaccttctctgggttctcactcagcactggtggagtgggtgtgggctggatccgtcagcccccaggaaaggccctggagtggcttgcactcatttattGgggatgatgataagcgctacagcccatctctgaagagcagactcaccatcaccaaggacacgtccaagaaccaggtggtcctcacaatgaccaacatggaccctgtggacacagccacatattactgtgcacacaggaggatacgattcggctttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccctggcaccctcctccaagagcacctctgggggcacagcgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaaact NA IGG1 93 88 [308, 309] a103>g,S35>G(+ + -)|g225>a|c246>g|a252>g|t267>c| a103>g,S35>G(+ + -)| g225>a|c246>g|a252>g|t267>c| a321>g|c322>a| 320 (322) 251 (253) 246 5 (7) 4 (6) 1 2 (3) 1 0 1 0 0 (1) 0 0 0 1 0 0 78 27 27 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 29 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 110 4 4 0 1 1 0 1 0 0 0 0 0 1 0 0 8 (10) 8 8 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 0 aa,13-14(FR1)|aa,40-41(FR1)|ta,102-103(CDR1)|aa,141-142(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,238-239(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,267-268(FR3)|aa,270-271(FR3)|aa,277-278(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,10-11(FR1)|tt,73-74(FR1)|tt,82-83(CDR1)|ta,102-103(CDR1)|tt,158-159(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,80-83(CDR1)|ggct,118-121(FR2)|ggct,155-158(FR2)|agca,220-223(FR3)|ggct,224-227(FR3)| aacc,41-44(FR1)|agcc,131-134(FR2)|agcc,202-205(FR3)|aacc,253-256(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)| accctcacgctgacctgcaccttctct gggttctcactcagcactggtggagtgggt gtgggctggatccgtcagcccccaggaaaggccctggagtggcttgcactc atttattgggatgatgataag cgctacagcccatctctgaagagcagactcaccatcaccaaggacacgtccaagaaccaggtggtcctcacaatgaccaacatggaccctgtggacacagccacatattactgt gcacacaggaggatacgattcggctttgactac AHRRIRFGFDY IGHV2-5 IGHD5-12 IGHJ4 gggttctcactcagcactggtggagtgggt gtgggctggatccgtcagcccccaggaaaggccctggagtggcttgcactc atttattgggatgatgataag cgctacagcccatctctgaagagcagactcaccatcaccaaggacacgtccaagaaccaggtggtcctcacaatgaccaacatggaccctgtggacacagccacatattactgt gcacacaggaggatacgattcggctttgactac IGG1 5 216 4 1 1 2 3 3 4 0 0 1 JY8QFUQ01B4Y1N 7071 productive Homsap IGHV6-1*01 F, or Homsap IGHV6-1*02 F 1003 94.22 212/225 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 10 9 14 10.9.14 [1.17.38.11] CARGGDYYDSGGLDYW in-frame + tccggggacagtgtctctagcagcagtgttgtttggaactggatcaggcagtccccattgagaggccttgagtggctgggaaggacattctacaggtccaggtggtataatgattattcattatctctgaaaagtcgaataaccatcaacccagacgcatccaagaaccagttgtccctgcagctgaagtctgtgactcccgaggacacggctgtatattactgtgcaagagggggtgattattatgatagtggtggtcttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [298] a104>g,N35>S(- - -)|c110>t,A37>V(+ - +)|c113>t,A38>V(+ - +)|c140>t,S47>L(- - -)|a170>t,Y57>F(- + -)|a185>g,K62>R(+ + +)|g202>t,A68>S(- + -)|g205>t,V69>L(+ - +)|g211>c,V71>L(+ - +)|a244>g,T82>A(- - -)|c261>g,F87>L(+ - -)|c276>g,N92>K(+ - -)|g303>a| a104>g,N35>S(- - -)|c110>t,A37>V(+ - +)|c113>t,A38>V(+ - +)| c140>t,S47>L(- - -)| a170>t,Y57>F(- + -)|a185>g,K62>R(+ + +)| g202>t,A68>S(- + -)|g205>t,V69>L(+ - +)|g211>c,V71>L(+ - +)|a244>g,T82>A(- - -)|c261>g,F87>L(+ - -)|c276>g,N92>K(+ - -)|g303>a| a320>g| 319 (320) 232 (233) 219 13 (14) 1 (2) 12 3 (4) 1 3 0 0 0 1 0 1 2 2 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 1 0 2 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 27 25 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 117 114 107 7 1 6 1 1 0 0 0 0 0 0 1 2 2 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,5-6(FR1)|aa,40-41(FR1)|aa,103-104(CDR1)|aa,118-119(FR2)|aa,162-163(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,184-185(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|ta,206-207(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,227-228(FR3)|aa,235-236(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,5-6(FR1)|tt,114-115(CDR1)|tt,149-150(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|tt,198-199(FR3)|ta,206-207(FR3)|ta,227-228(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggta,3-6(FR1)|agct,8-11(FR1)|agca,14-17(FR1)|agca,100-103(CDR1)|ggca,128-131(FR2)|ggct,155-158(FR2)|ggta,188-191(CDR2)|agta,204-207(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tgcc,69-72(FR1)|tgct,108-111(CDR1)|aact,118-121(FR2)|tact,169-172(CDR2)|aacc,228-231(FR3)|aacc,235-238(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|aact,274-277(FR3)|tact,307-310(FR3)| tcc ggggacagtgtctctagcagcagtgttgtt tggaactggatcaggcagtccccattgagaggccttgagtggctgggaagg acattctacaggtccaggtggtataat gattattcattatctctgaaaagtcgaataaccatcaacccagacgcatccaagaaccagttgtccctgcagctgaagtctgtgactcccgaggacacggctgtatattactgt gcaagagggggtgattattatgatagtggtggtcttgactac ARGGDYYDSGGLDY IGHV6-1 IGHD3-22 IGHJ4 ggggacagtgtctctagcagcagtgttgtt tggaactggatcaggcagtccccattgagaggccttgagtggctgggaagg acattctacaggtccaggtggtataat gattattcattatctctgaaaagtcgaataaccatcaacccagacgcatccaagaaccagttgtccctgcagctgaagtctgtgactcccgaggacacggctgtatattactgt gcaagagggggtgattattatgatagtggtggtcttgactac IGA1 13 222 7 6 4 9 3 4 1 0 7 5 JY8QFUQ01B50HH 17740 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 909 91.24 198/217 nt NA Homsap IGHJ6*02 F (see comment) 103 62.9 39/62 nt Homsap IGHD2-2*01 F 3 8 8 20 8.8.20 [1.17.38.11] CAKTVVPAAPNRFYYYYGLDVW in-frame + Other possibilities: Homsap_IGHJ1*01 (highest number of consecutive identical nucleotides) or Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ctcaggattcacgtttagcaggtatgccatgaactgggtccgccaggctccagagaaggggctggagtgggtctccgctgttagtgatactggtggtacaacagagtacgccgactccgtgaaggggcggttcaccgtctccagagacaattccaagaatacggtgtatctgcaaatgaacagcctgagagtcgaggacacggccgtatattactgtgcgaagaccgtagtgccagctgctcctaatcgattttactactactacggtttggacgtctggggcccaggggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [310] t78>a|c87>g|c108>g,S36>R(- - -)|g119>a,S40>N(- - -)|g140>a,G47>E(- - -)|a162>c|a166>g,I56>V(+ - +)|g173>a,G58>D(- - -)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)|t196>g,Y66>E(- - -)|c198>g,Y66>E(- - -)|a204>c|c222>g|a232>g,I78>V(+ - +)|c255>t|c259>g,L87>V(+ - +)|c287>t,A96>V(+ - +)| t78>a| c87>g|c108>g,S36>R(- - -)| g119>a,S40>N(- - -)|g140>a,G47>E(- - -)|a162>c| a166>g,I56>V(+ - +)|g173>a,G58>D(- - -)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)| t196>g,Y66>E(- - -)|c198>g,Y66>E(- - -)|a204>c|c222>g|a232>g,I78>V(+ - +)|c255>t|c259>g,L87>V(+ - +)|c287>t,A96>V(+ - +)| a318>g|g319>a|a320>c| 317 (320) 222 (225) 203 19 (22) 6 (9) 13 2 (3) 3 (4) 2 0 2 (3) 1 0 1 2 5 0 1 78 4 3 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 22 2 1 1 0 0 0 0 0 0 0 0 0 2 0 0 51 51 48 3 1 2 0 2 0 0 1 0 0 0 0 0 0 0 30 24 19 5 0 5 1 1 0 0 0 1 0 0 2 0 0 0 117 114 106 8 3 5 1 0 2 0 1 0 0 0 0 3 0 1 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctca ggattcacgtttagcaggtatgcc atgaactgggtccgccaggctccagagaaggggctggagtgggtctccgct gttagtgatactggtggtacaaca gagtacgccgactccgtgaaggggcggttcaccgtctccagagacaattccaagaatacggtgtatctgcaaatgaacagcctgagagtcgaggacacggccgtatattactgt gcgaagaccgtagtgccagctgctcctaatcgattttactactactacggtttggacgtc AKTVVPAAPNRFYYYYGLDV IGHV3-23 IGHD2-2 IGHJ6 ggattcacgtttagcaggtatgcc atgaactgggtccgccaggctccagagaaggggctggagtgggtctccgct gttagtgatactggtggtacaaca gagtacgccgactccgtgaaggggcggttcaccgtctccagagacaattccaagaatacggtgtatctgcaaatgaacagcctgagagtcgaggacacggccgtatattactgt gcgaagaccgtagtgccagctgctcctaatcgattttactactactacggtttggacgtc IGG2 18 213 7 11 5 13 2 5 4 1 7 6 JY8QFUQ01B53SB 17086 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F (see comment) 696 80.84 173/214 nt 80.37 172/214 nt Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD6-6*01 F 3 8 7 9 8.7.9 [1.17.38.11] CARLDGHFDLW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (80,84% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR3-IMGT at codon 74 of V-REGION: 'G' (1 nucleotides at position 122 to 122 in the user submitted sequence), (cause frameshift); ctctgggttcaccatcagtcactactccatggcctgggtccgccaggctccgggaaaggggctggagtgggtctcattaatttatcccggcgggagcacattccacgcagactccgtgaggGggcggcttatcgatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgtgcgagactcgacgggcacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [274] g88>a,V30>I(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g|a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c223>g,R75>G(- - -)|a225>c,R75>G(- - -)|c228>a,F76>L(+ - -)|a229>t,T77>S(+ - +)|c231>g,T77>S(+ - +)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| g88>a,V30>I(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g| a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c223>g,R75>G(- - -)|a225>c,R75>G(- - -)|c228>a,F76>L(+ - -)|a229>t,T77>S(+ - +)|c231>g,T77>S(+ - +)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| g319>c|a320>t| 318 (320) 220 (222) 179 41 (43) 7 (9) 34 8 8 3 2 4 3 3 (4) 2 2 (3) 4 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 2 0 0 2 0 1 0 0 0 0 0 51 51 45 6 2 4 2 1 0 0 0 0 0 1 1 0 1 0 30 21 17 4 2 2 0 0 0 1 1 0 0 0 1 0 0 1 117 114 88 26 3 23 6 5 3 1 1 3 2 1 0 4 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcaccatcagtcactactcc atggcctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgaggggcggcttatcgatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc ARLDGHFDL IGHV3-53 IGHD6-6 IGHJ2 gggttcaccatcagtcactactcc atggcctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgaggggcggcttatcgatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc IGG1 41 210 21 20 11 21 10 20 5 2 27 7 JY8QFUQ01B569F 2419 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 958 93.98 203/216 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD6-6*01 F 2 8 8 16 8.8.16 [1.17.38.11] CAFSRALTGKWIDYLDYW in-frame + tctggattcatcttcagcaactactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtattactggtgatgggagtaacccaatctacgcggaccccgtgaagggtcgattcaccatctccagagacaacgccaagaacacactatatctgcaaatgaacagtctgagagtcgaggacacggctgtgtattactgtgcctttagtagagcactcacggggaagtggatcgactaccttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttct NA IGA1 100 100 [295] c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)|a170>c,N57>T(- + -)|a172>g,S58>G(+ + -)|g191>a,S64>N(- - -)|a193>c,T65>P(+ + -)|g197>t,S66>I(- - -)|t208>c,S70>P(+ - -)|c222>t|g258>a|g261>a|c287>t,A96>V(+ - +)| c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)| a170>c,N57>T(- + -)|a172>g,S58>G(+ + -)|g191>a,S64>N(- - -)|a193>c,T65>P(+ + -)| g197>t,S66>I(- - -)|t208>c,S70>P(+ - -)|c222>t|g258>a|g261>a|c287>t,A96>V(+ - +)| a315>c|a316>t,R106>F(- - -)|g317>t,R106>F(- - -)|a318>t,R106>F(- - -)|g319>a|a320>g| 314 (320) 218 (224) 205 13 (19) 4 (7) 9 (12) 1 (2) 4 (5) 3 2 2 (3) 0 0 (2) 0 0 0 1 (2) 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 1 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 1 0 0 2 0 0 0 0 0 0 0 117 114 108 6 3 3 0 2 2 1 0 0 0 0 0 0 1 0 2 (8) 2 2 0 (6) 0 (3) 0 (3) 0 (1) 0 (1) 0 0 0 (1) 0 0 (2) 0 0 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcatcttcagcaactactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attactggtgatgggagtaaccca atctacgcggaccccgtgaagggtcgattcaccatctccagagacaacgccaagaacacactatatctgcaaatgaacagtctgagagtcgaggacacggctgtgtattactgt gcctttagtagagcactcacggggaagtggatcgactaccttgactac AFSRALTGKWIDYLDY IGHV3-74 IGHD6-6 IGHJ4 ggattcatcttcagcaactactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attactggtgatgggagtaaccca atctacgcggaccccgtgaagggtcgattcaccatctccagagacaacgccaagaacacactatatctgcaaatgaacagtctgagagtcgaggacacggctgtgtattactgt gcctttagtagagcactcacggggaagtggatcgactaccttgactac IGA1 13 213 10 3 7 8 3 5 3 1 3 6 JY8QFUQ01B56F9 14870 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 896 90.37 197/218 nt NA Homsap IGHJ5*02 F 228 94.12 48/51 nt Homsap IGHD6-6*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVRSEYSSSNWFDPW in-frame + cctctggattcagcctcattgactttagaatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattgatagtgttggtcaaaacatatactacagagactcagtgcggggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgtgagatctgagtatagtagttcaaactggttcgacccctggggccaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [288] c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|g104>t,S35>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>t,Y37>F(- + -)|c114>a,S38>R(- - -)|a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|a184>g,S62>G(+ + -)|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>a,S63>Q(- - -)|t190>a,Y64>N(- - -)|g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|a214>c,K72>R(+ + +)|a215>g,K72>R(+ + +)|a240>g|c314>t,A105>V(+ - +)| c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|g104>t,S35>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>t,Y37>F(- + -)|c114>a,S38>R(- - -)| a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|a184>g,S62>G(+ + -)|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>a,S63>Q(- - -)|t190>a,Y64>N(- - -)| g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|a214>c,K72>R(+ + +)|a215>g,K72>R(+ + +)|a240>g| c314>t,A105>V(+ - +)|g319>t|a320>c| 318 (320) 224 (226) 202 22 (24) 1 (3) 21 6 4 1 1 2 (3) 1 1 2 0 2 2 (3) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 0 7 1 1 0 1 0 1 1 0 0 1 1 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 15 9 0 9 3 2 0 0 1 0 0 2 0 0 1 0 117 114 109 5 1 4 2 1 0 0 1 0 0 0 0 1 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcagcctcattgactttaga atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attgatagtgttggtcaaaacata tactacagagactcagtgcggggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gtgagatctgagtatagtagttcaaactggttcgacccc VRSEYSSSNWFDP IGHV3-21 IGHD6-6 IGHJ5 ggattcagcctcattgactttaga atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attgatagtgttggtcaaaacata tactacagagactcagtgcggggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gtgagatctgagtatagtagttcaaactggttcgacccc IGA2 21 213 11 10 4 9 7 12 1 0 4 16 JY8QFUQ01B56VC 4589 productive (see comment) Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 952 92.34 205/222 nt 91.89 204/222 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD5-24*01 ORF 1 8 10 10 8.10.10 [1.17.37.11] CTHLREEMSYEW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 84 of V-REGION: 1 nucleotides (from position 161 in the user submitted sequence), (cause frameshift) ctctggagtcaaattcagaaacgcctggatgaattgggtccgccaggctccagggaaggggctggagtgggttggccgtattaagagcaaagctgatggtgggacaacagactacgccacacccgtgagaggcagattcaccatctcaagagatgattcaaaaacacgttttatctgcaaatgaatagcctaaaaaccgaagacacagccgtctattactgtacccacttgcgagaagagatgtcttacgaatggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [283] t82>g,F28>V(+ - -)|c86>a,T29>K(- - -)|t87>a,T29>K(- - -)|t105>a,S35>R(- - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a171>g|a178>g,T60>A(- - -)|t204>c|g205>a,A69>T(- - -)|a215>g,K72>R(+ + +)|c259>t,L87>F(+ - -)|g261>t,L87>F(+ - -)|c276>t|g282>a|g291>a|g303>c| t82>g,F28>V(+ - -)|c86>a,T29>K(- - -)|t87>a,T29>K(- - -)|t105>a,S35>R(- - -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)| a171>g|a178>g,T60>A(- - -)| t204>c|g205>a,A69>T(- - -)|a215>g,K72>R(+ + +)|c259>t,L87>F(+ - -)|g261>t,L87>F(+ - -)|c276>t|g282>a|g291>a|g303>c| a316>c,T106>H(+ - -)|c317>a,T106>H(+ - -)|a318>c,T106>H(+ - -)|g319>t|a320>t| 315 (320) 225 (230) 208 17 (22) 6 (8) 11 (14) 3 4 3 1 0 (2) 1 (2) 0 (1) 2 1 0 1 (2) 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 0 0 1 0 2 0 0 0 1 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 30 28 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 117 113 104 9 5 4 1 3 2 1 0 0 0 0 1 0 1 0 3 (8) 3 3 0 (5) 0 (2) 0 (3) 0 0 0 0 0 (2) 0 (1) 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| ctct ggagtcaaattcagaaacgcctgg atgaattgggtccgccaggctccagggaaggggctggagtgggttggccgt attaagagcaaagctgatggtgggacaaca gactacgccacacccgtgagaggcagattcaccatctcaagagatgattcaaaaacacgttttatctgcaaatgaatagcctaaaaaccgaagacacagccgtctattactgt acccacttgcgagaagagatgtcttacgaa THLREEMSYE IGHV3-15 IGHD5-24 IGHJ4 ggagtcaaattcagaaacgcctgg atgaattgggtccgccaggctccagggaaggggctggagtgggttggccgt attaagagcaaagctgatggtgggacaaca gactacgccacacccgtgagaggcagattcaccatctcaagagatgattcaaaaacacgttttatctgcaaatgaatagcctaaaaaccgaagacacagccgtctattactgt acccacttgcgagaagagatgtcttacgaa IGA1 17 218 11 6 7 10 4 7 5 1 6 5 JY8QFUQ01B58W5 19349 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 927 92.17 200/217 nt NA Homsap IGHJ5*02 F 111 68.63 35/51 nt Homsap IGHD3-3*01 F 2 8 8 21 8.8.21 [1.17.38.11] CVRVGAPYYDFWSGYQTNWFDPW in-frame + gtctggattcagcatcagtagttatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattagtgctagtactacttccatacactatgcagactcagtgaagggccgattcaccatctccagagacgacgccaagagttccctgtatttgcaaatgaacagcctgagagccgaggacacggctgtatattactgtgtaagagtgggggcgccatattacgatttctggagtggctatcagactaactggttcgacccctggggccagggaactctgtcaccgttacctcagcatccccgaccagcccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccaggcttcttcc NA IGA1 100 95 [311, 310] c75>g|c86>g,T29>S(+ - +)|t88>a,F30>I(+ - -)|c108>t|a172>g,S58>A(- + -)|g173>c,S58>A(- + -)|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|a191>c,Y64>S(+ - -)|t196>c,Y66>H(+ - -)|c201>t|a244>g,N82>D(+ + -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a258>c|c265>t|g303>a|c314>t,A105>V(+ - +)|g315>a,A105>V(+ - +)| c75>g| c86>g,T29>S(+ - +)|t88>a,F30>I(+ - -)|c108>t| a172>g,S58>A(- + -)|g173>c,S58>A(- + -)|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|a191>c,Y64>S(+ - -)| t196>c,Y66>H(+ - -)|c201>t|a244>g,N82>D(+ + -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a258>c|c265>t|g303>a| c314>t,A105>V(+ - +)|g315>a,A105>V(+ - +)|a320>t| 319 (320) 224 (225) 205 19 (20) 6 (7) 13 3 2 5 1 2 0 0 (1) 1 3 2 0 0 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 21 3 1 2 0 0 1 0 0 0 0 1 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 19 5 0 5 1 0 0 0 1 0 0 0 3 0 0 0 117 114 106 8 4 4 2 1 3 1 1 0 0 0 0 0 0 0 7 (8) 7 5 2 (3) 0 (1) 2 0 1 1 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcagcatcagtagttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtgctagtactacttccata cactatgcagactcagtgaagggccgattcaccatctccagagacgacgccaagagttccctgtatttgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gtaagagtgggggcgccatattacgatttctggagtggctatcagactaactggttcgacccc VRVGAPYYDFWSGYQTNWFDP IGHV3-21 IGHD3-3 IGHJ5 ggattcagcatcagtagttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtgctagtactacttccata cactatgcagactcagtgaagggccgattcaccatctccagagacgacgccaagagttccctgtatttgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gtaagagtgggggcgccatattacgatttctggagtggctatcagactaactggttcgacccc IGA1 16 213 9 7 5 9 4 7 4 1 4 7 JY8QFUQ01B5F34 18834 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 869 88.99 194/218 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD2-15*01 F 3 8 8 13 8.8.13 [1.17.38.11] CARVFSGVAAPPDSW in-frame - cctctggatttcccttcaccagttactggatgcagtgggtccgccaagctccagggaaggggctggtctgggtctcacttagtaatagtgatgggagcactactacctacgcggacgccgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtatcttcaaatgaatcatctgagagtcgaggacaccgctgtatatttctgtgcgagagttttcagtggtgtcgctgctcccccggactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [288] c84>t|a85>c,T29>P(+ + -)|g104>c,S35>T(+ - +)|t105>c,S35>T(+ - +)|c108>t|c120>g,H40>Q(- + -)|g153>c|g164>t,R55>L(- + -)|t167>g,I56>S(- - -)|t189>c|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|a195>t|g197>c,S66>T(+ - +)|t208>g,S70>A(- + -)|g258>a|g267>t|c276>t|a277>c,S93>H(+ - -)|g278>a,S93>H(+ - -)|c287>t,A96>V(+ - +)|g297>c|g303>a|a308>t,Y103>F(- + -)|a315>g| c84>t|a85>c,T29>P(+ + -)|g104>c,S35>T(+ - +)|t105>c,S35>T(+ - +)|c108>t| c120>g,H40>Q(- + -)|g153>c|g164>t,R55>L(- + -)| t167>g,I56>S(- - -)|t189>c|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|a195>t| g197>c,S66>T(+ - +)|t208>g,S70>A(- + -)|g258>a|g267>t|c276>t|a277>c,S93>H(+ - -)|g278>a,S93>H(+ - -)|c287>t,A96>V(+ - +)|g297>c|g303>a|a308>t,Y103>F(- + -)| a315>g|a320>t| 319 (320) 225 (226) 200 25 (26) 11 (12) 14 1 3 5 2 2 0 2 (3) 0 5 1 2 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 0 2 1 1 0 0 0 1 0 0 0 51 51 48 3 1 2 0 0 0 0 0 0 0 0 1 1 1 0 30 24 19 5 2 3 0 0 1 1 0 0 1 0 1 0 0 1 117 114 103 11 5 6 0 3 2 0 1 0 1 0 2 0 1 1 7 (8) 7 6 1 (2) 1 (2) 0 1 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggatttcccttcaccagttactgg atgcagtgggtccgccaagctccagggaaggggctggtctgggtctcactt agtaatagtgatgggagcactact acctacgcggacgccgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtatcttcaaatgaatcatctgagagtcgaggacaccgctgtatatttctgt gcgagagttttcagtggtgtcgctgctcccccggactcc ARVFSGVAAPPDS IGHV3-74 IGHD2-15 IGHJ4 ggatttcccttcaccagttactgg atgcagtgggtccgccaagctccagggaaggggctggtctgggtctcactt agtaatagtgatgggagcactact acctacgcggacgccgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtatcttcaaatgaatcatctgagagtcgaggacaccgctgtatatttctgt gcgagagttttcagtggtgtcgctgctcccccggactcc IGA1 24 213 10 14 8 16 2 8 6 4 8 6 JY8QFUQ01B5GFJ 19060 productive (see comment) Homsap IGHV3-7*01 F 957 94.39 202/214 nt 93.93 201/214 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-22*01 F 2 8 8 14 8.8.14 [0.17.38.11] CARGSYYYYDTNGDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'G' (1 nucleotides at position 54 to 54 in the user submitted sequence), (cause frameshift); tggatttacctttacaaattattggatgagctgggtccgccaggctccagggaGgggggctggagtgggtggccagcgtaaaacaagatggaggtgagaaatactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagggtcctactattattatgatactaatggcgactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [288] c84>t|g104>c,S35>T(+ - +)|t105>a,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a143>g,K48>R(+ + +)|a164>g,N55>S(- - -)|a166>g,I56>V(+ - +)|g171>a|a187>g,S63>G(+ + -)|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)| c84>t|g104>c,S35>T(+ - +)|t105>a,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a143>g,K48>R(+ + +)|a164>g,N55>S(- - -)| a166>g,I56>V(+ - +)|g171>a|a187>g,S63>G(+ + -)| a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)| a320>g| 319 (320) 221 (222) 209 12 (13) 2 (3) 10 5 (6) 2 2 0 1 0 0 1 1 0 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 2 0 0 0 0 1 1 0 0 0 51 51 49 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 2 1 0 0 0 0 0 0 0 0 0 0 117 114 112 2 0 2 1 0 0 0 1 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t ggatttacctttacaaattattgg atgagctgggtccgccaggctccagggagggggctggagtgggtggccagc gtaaaacaagatggaggtgagaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggtcctactattattatgatactaatggcgactat ARGSYYYYDTNGDY IGHV3-7 IGHD3-22 IGHJ4 ggatttacctttacaaattattgg atgagctgggtccgccaggctccagggagggggctggagtgggtggccagc gtaaaacaagatggaggtgagaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggtcctactattattatgatactaatggcgactat IGA1 12 213 9 3 4 5 5 7 0 2 4 6 JY8QFUQ01B5QDY 19458 productive Homsap IGHV3-7*01 F 887 89.91 196/218 nt NA Homsap IGHJ6*02 F (see comment) 103 62.9 39/62 nt Homsap IGHD3-22*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARDPSYSYYNGLDVW in-frame + Other possibilities: Homsap_IGHJ4*03 (shorter alignment but highest percentage of identity) cctctaaattcacttttagtaactattggatgaattgggtccgccaggctccagcgaagggactggagtgggtggccagtataaagcaggatgggggggagacatattatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaagtcactgtatctgcaaatgaacagcctgggagtcgaagacacggctgtttattactgtgcgagagatccatcttactcctactacaatggtttggacgtctggggccaaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtcccagggcttcttccccc NA IGA2 100 100 [292] g79>a,G27>K(- - -)|g80>a,G27>K(- - -)|c87>t|g107>a,S36>N(- - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g140>c,G47>A(- + -)|g147>a|a164>g,N55>S(- - -)|c165>t,N55>S(- - -)|a174>g|a186>g|a187>g,S63>G(+ + -)|t189>g,S63>G(+ + -)|a194>c,K65>T(- - -)|c198>t|t203>c,V68>A(+ - +)|c255>g,N85>K(+ - -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g291>a|g303>t| g79>a,G27>K(- - -)|g80>a,G27>K(- - -)|c87>t|g107>a,S36>N(- - -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g140>c,G47>A(- + -)|g147>a|a164>g,N55>S(- - -)|c165>t,N55>S(- - -)| a174>g|a186>g|a187>g,S63>G(+ + -)|t189>g,S63>G(+ + -)|a194>c,K65>T(- - -)| c198>t|t203>c,V68>A(+ - +)|c255>g,N85>K(+ - -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g291>a|g303>t| 320 226 204 22 7 15 5 6 5 1 1 0 0 0 1 1 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 3 1 0 0 0 0 0 0 0 0 0 51 51 45 6 1 5 1 2 2 0 0 0 0 0 1 0 0 0 30 24 19 5 2 3 3 0 0 0 1 0 0 0 0 0 0 1 117 114 107 7 3 4 1 1 2 1 0 0 0 0 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct aaattcacttttagtaactattgg atgaattgggtccgccaggctccagcgaagggactggagtgggtggccagt ataaagcaggatgggggggagaca tattatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaagtcactgtatctgcaaatgaacagcctgggagtcgaagacacggctgtttattactgt gcgagagatccatcttactcctactacaatggtttggacgtc ARDPSYSYYNGLDV IGHV3-7 IGHD3-22 IGHJ6 aaattcacttttagtaactattgg atgaattgggtccgccaggctccagcgaagggactggagtgggtggccagt ataaagcaggatgggggggagaca tattatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaagtcactgtatctgcaaatgaacagcctgggagtcgaagacacggctgtttattactgt gcgagagatccatcttactcctactacaatggtttggacgtc IGA2 22 213 17 5 11 14 6 8 4 3 9 6 JY8QFUQ01B5SAT 11930 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 1080 100 217/217 nt 98.62 214/217 nt Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD3-22*01 F 3 8 8 14 8.8.14 [1.16.38.11] CARDFFIGGYYGMDVW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'G' (1 nucleotides at position 15 to 15 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 43 of V-REGION: 'G' (1 nucleotides at position 45 to 45 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 59 in the user submitted sequence), (cause frameshift) cctctggattcaccGttcagtagctatagcatgaactgggtccgGccaggctccagggaagggctggagtgggtctcatccattagtagtagtagtagttacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagatttcttcataggaggctactacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [292] 320 225 225 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 50 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaagggctggagtgggtctcatcc attagtagtagtagtagttacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagatttcttcataggaggctactacggtatggacgtc ARDFFIGGYYGMDV IGHV3-21 IGHD3-22 IGHJ6 ggattcaccttcagtagctatagc atgaactgggtccgccaggctccagggaagggctggagtgggtctcatcc attagtagtagtagtagttacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagatttcttcataggaggctactacggtatggacgtc IGA1 0 212 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01B5VR4 5839 productive Homsap IGHV1-24*01 F 970 95.31 203/213 nt NA Homsap IGHJ5*02 F 141 77.08 37/48 nt Homsap IGHD5-18*01 F 1 8 8 7 8.8.7 [X.17.38.11] CAIGAMATW in-frame + cggaaaaccctcactgaagtatccatgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggaggatttgatcctgaagatggtgaaataatctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgtgcaataggagctatggccacctggggccagggaaccctggtcaccgtcgactcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgcaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccaa NA IGA2 71 95 [265, 267] g79>c,G27>R(- - -)|a81>g,G27>R(- - -)|t82>a,Y28>K(- - -)|c84>a,Y28>K(- - -)|t109>g,L37>V(+ - +)|t165>a|c194>t,T65>I(- - -)|g226>a,V76>I(+ - +)|a232>g,M78>V(+ - -)|g291>a|c317>t,T106>I(- - -)| g79>c,G27>R(- - -)|a81>g,G27>R(- - -)|t82>a,Y28>K(- - -)|c84>a,Y28>K(- - -)|t109>g,L37>V(+ - +)| t165>a| c194>t,T65>I(- - -)| g226>a,V76>I(+ - +)|a232>g,M78>V(+ - -)|g291>a| c317>t,T106>I(- - -)|a320>g| 319 (320) 220 (221) 209 11 (12) 2 (3) 9 2 (3) 2 2 0 0 1 0 2 1 0 0 1 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 0 0 0 0 1 0 1 1 0 0 1 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 117 114 111 3 1 2 1 2 0 0 0 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,14-15(FR1)|aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|aa,107-108(CDR1)|ta,110-111(CDR1)|aa,141-142(FR2)|aa,143-144(FR2)|aa,176-177(CDR2)|aa,191-192(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,249-250(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,14-15(FR1)|tt,74-75(FR1)|ta,82-83(CDR1)|tt,109-110(CDR1)|tt,149-150(FR2)|tt,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,249-250(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggta,12-15(FR1)|ggct,24-27(FR1)|ggtt,72-75(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,163-166(FR2-CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| cggaaaaccctcactgaagtatcc atgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggagga tttgatcctgaagatggtgaaata atctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcaataggagctatggccacc AIGAMAT IGHV1-24 IGHD5-18 IGHJ5 cggaaaaccctcactgaagtatcc atgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggagga tttgatcctgaagatggtgaaata atctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcaataggagctatggccacc IGA2 10 213 5 5 3 5 2 5 2 0 2 6 JY8QFUQ01B5XE3 13249 productive (see comment) Homsap IGHV4-61*02 F 829 86.76 190/219 nt 86.3 189/219 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-18*01 F 3 9 7 15 9.7.15 [1.17.38.11] CARDTGQTYGRGPLDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 30 of V-REGION: 1 nucleotides (from position 16 in the user submitted sequence), (cause frameshift) ctctggtgcctccataataggggtaattattactggaactggatccggcagcccgccgggaagggcctggaatggattggccgaatctatacaagtgggagcaccatctacaacccctccctcgggggtcgagtcaccatgactgtagacccgtccgagaatcagttcttcctgagactgagttctgtgaccgccgcagacacggccgtttatttttgtgcgagagacactggacaaacctacggtcggggccctttggactactggggccagggaatcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [296] g83>c,G28>A(- + -)|g92>a,S31>N(- - -)|c93>t,S31>N(- - -)|t102>g,S34>R(- - -)|g107>a,S36>N(- - -)|c111>t|g119>a,S40>N(- - -)|a147>c|g153>a|g162>c|t165>a|c174>a|a197>t,N66>I(- - -)|a214>g,K72>G(- - -)|a215>g,K72>G(- - -)|a220>g,S74>G(+ + -)|a234>g,I78>M(+ + -)|t235>a,S79>T(+ - +)|a237>t,S79>T(+ - +)|a244>c,T82>P(+ + -)|a250>g,K84>E(+ - -)|c255>t|c263>t,S88>F(- - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c276>t|g303>t|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g83>c,G28>A(- + -)|g92>a,S31>N(- - -)|c93>t,S31>N(- - -)|t102>g,S34>R(- - -)|g107>a,S36>N(- - -)|c111>t| g119>a,S40>N(- - -)|a147>c|g153>a|g162>c|t165>a| c174>a| a197>t,N66>I(- - -)|a214>g,K72>G(- - -)|a215>g,K72>G(- - -)|a220>g,S74>G(+ + -)|a234>g,I78>M(+ + -)|t235>a,S79>T(+ - +)|a237>t,S79>T(+ - +)|a244>c,T82>P(+ + -)|a250>g,K84>E(+ - -)|c255>t|c263>t,S88>F(- - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c276>t|g303>t|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| 320 227 198 29 9 20 6 5 6 0 2 1 3 2 2 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 29 23 6 1 5 0 2 2 0 0 0 0 0 1 0 0 1 51 51 46 5 4 1 0 2 0 0 1 0 0 1 1 0 0 0 30 21 20 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 117 114 97 17 3 14 6 1 4 0 1 0 3 1 0 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtgcctccataataggggtaattattac tggaactggatccggcagcccgccgggaagggcctggaatggattggccga atctatacaagtgggagcacc atctacaacccctccctcgggggtcgagtcaccatgactgtagacccgtccgagaatcagttcttcctgagactgagttctgtgaccgccgcagacacggccgtttatttttgt gcgagagacactggacaaacctacggtcggggccctttggactac ARDTGQTYGRGPLDY IGHV4-61 IGHD5-18 IGHJ4 ggtgcctccataataggggtaattattac tggaactggatccggcagcccgccgggaagggcctggaatggattggccga atctatacaagtgggagcacc atctacaacccctccctcgggggtcgagtcaccatgactgtagacccgtccgagaatcagttcttcctgagactgagttctgtgaccgccgcagacacggccgtttatttttgt gcgagagacactggacaaacctacggtcggggccctttggactac IGG1 29 215 17 12 11 15 6 14 7 2 15 5 JY8QFUQ01B5XEH 586 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F (see comment) 677 95.3 142/149 nt 94.63 141/149 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 1 8 8.1.8 [1.1.38.11] CARDNYGDYW in-frame + Potentially, because of detected insertion/deletion The submitted sequence and the closest germline V-GENE allele show different CDR2-IMGT amino acid lengths (1 AA in JY8QFUQ01B5XEH ; 8 AA in Homsap_IGHV3-74*01) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR2-IMGT, from codon 40 of V-REGION: 68 nucleotides (from position 33 in the user submitted sequence), (cause frameshift) ctctggattcactttcagtagctactggatgcacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcaccgtctcctcagcatcccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccaa NA IGA2 100 95 [203] c87>t|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| c87>t| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| 320 157 150 7 5 2 1 1 1 0 0 0 0 0 3 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 4 2 1 1 0 0 0 0 0 0 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcactttcagtagctactgg atgc aca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcactttcagtagctactgg atgc aca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac IGA2 7 145 3 4 2 6 1 1 4 1 2 0 JY8QFUQ01B641M 12781 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 1031 97.25 212/218 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD1-26*01 F 3 8 8 18 8.8.18 [1.17.38.11] CAKELGRALTGNYYQYFHYW in-frame + cctctggattcacgttcagtggctatggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagttgtatcatatgatggaagtaataaatactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctctctctgcaaatgaacagcctgagagctgaggacacggctgtatattactgtgcgaaagaattggggagggccctgactgggaactactaccagtactttcactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [303] c87>g|a106>g,S36>G(+ + -)|a166>g,I56>V(+ - +)|g261>c|a263>c,Y88>S(+ - -)|g303>a|g317>a,R106>K(+ + +)| c87>g|a106>g,S36>G(+ + -)| a166>g,I56>V(+ - +)| g261>c|a263>c,Y88>S(+ - -)|g303>a| g317>a,R106>K(+ + +)| 320 226 219 7 3 4 2 2 0 0 1 0 0 0 1 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 1 0 0 0 0 0 0 0 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 117 114 111 3 2 1 0 1 0 0 1 0 0 0 1 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacgttcagtggctatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt gtatcatatgatggaagtaataaa tactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctctctctgcaaatgaacagcctgagagctgaggacacggctgtatattactgt gcgaaagaattggggagggccctgactgggaactactaccagtactttcactac AKELGRALTGNYYQYFHY IGHV3-30 IGHD1-26 IGHJ4 ggattcacgttcagtggctatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt gtatcatatgatggaagtaataaa tactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctctctctgcaaatgaacagcctgagagctgaggacacggctgtatattactgt gcgaaagaattggggagggccctgactgggaactactaccagtactttcactac IGA2 6 213 3 3 1 3 2 3 2 1 1 2 JY8QFUQ01B64QD 21681 productive (see comment) Homsap IGHV4-34*03 F 885 90.61 193/213 nt 90.14 192/213 nt Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 228 94.12 48/51 nt Homsap IGHD3-22*01 F 2 8 7 16 8.7.16 [1.16.38.11] CVRNYYDFSGYFNWFDSW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR2-IMGT, from codon 45 of V-REGION: 1 nucleotides (from position 50 in the user submitted sequence), (cause frameshift) tgtctggtgggtccttcagtggttactactggagttggatccgccagccccagggaaggggctggagtggattggggaaatcaattatagaggaagtaccaactacaatccgtccctcaaaagtcgagtcaccgtgtcttcagacacgtccaagaatcagttctccctggagttgatctctgtgaccgccgcggacacggccatatattattgtgtgagaaattactatgattttagtggttatttcaattggttcgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [293] c75>g|a77>c,Y26>S(+ - -)|c120>t|c172>t,H58>Y(+ - -)|t177>a,S59>R(- - -)|c192>t|c204>t|g216>a|a232>g,I78>V(+ - +)|a234>g,I78>V(+ - +)|a237>t|g238>t,V80>S(- - -)|t239>c,V80>S(- - -)|c255>t|a268>g,K90>E(+ - -)|c271>t|g275>t,S92>I(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| c75>g|a77>c,Y26>S(+ - -)| c120>t| c172>t,H58>Y(+ - -)|t177>a,S59>R(- - -)|c192>t| c204>t|g216>a|a232>g,I78>V(+ - +)|a234>g,I78>V(+ - +)|a237>t|g238>t,V80>S(- - -)|t239>c,V80>S(- - -)|c255>t|a268>g,K90>E(+ - -)|c271>t|g275>t,S92>I(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| 311 213 193 20 9 11 3 3 7 1 1 0 1 1 0 1 2 0 78 5 3 2 1 1 0 0 0 0 1 0 0 0 0 1 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 50 49 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 21 18 3 1 2 0 0 2 0 0 0 0 1 0 0 0 0 116 114 99 14 6 8 3 3 4 1 0 0 1 0 0 0 2 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tgtct ggtgggtccttcagtggttactac tggagttggatccgccagccccagggaaggggctggagtggattggggaa atcaattatagaggaagtacc aactacaatccgtccctcaaaagtcgagtcaccgtgtcttcagacacgtccaagaatcagttctccctggagttgatctctgtgaccgccgcggacacggccatatattattgt gtgagaaattactatgattttagtggttatttcaattggttcgactcc VRNYYDFSGYFNWFDS IGHV4-34 IGHD3-22 IGHJ5 ggtgggtccttcagtggttactac tggagttggatccgccagccccagggaaggggctggagtggattggggaa atcaattatagaggaagtacc aactacaatccgtccctcaaaagtcgagtcaccgtgtcttcagacacgtccaagaatcagttctccctggagttgatctctgtgaccgccgcggacacggccatatattattgt gtgagaaattactatgattttagtggttatttcaattggttcgactcc IGA1 18 209 14 4 10 12 4 6 7 1 8 2 JY8QFUQ01B6832 11456 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 867 89.72 192/214 nt 89.25 191/214 nt Homsap IGHJ3*02 F 106 68 34/50 nt Homsap IGHD2-15*01 F 2 8 7 20 8.7.20 [1.17.37.11] CARDPRDYCSGGYCFVGAFDIW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 92 of V-REGION: 1 nucleotides (from position 177 in the user submitted sequence), (cause frameshift) tctctggtggctccatgaggaattattactggagctggatccggcagtccccagggaagggactggagttgatagggactgtctattacactgggcgcacggagtacaacccctccctcaagagtcgactcaccttatcactagacacgtccaagaaccagttctccctaaagctggctctgtgaccgctgcggactcggccatttattactgtgcgagagacccccgagactattgtagtggtggttactgttttgttggtgcttttgatatctggggccaagggacaaatggtcaccgtctcttcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttgccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [307] c90>g,I30>M(+ + -)|t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t|c133>t,P45>S(+ - -)|g155>t,W52>L(+ - -)|t159>a|t163>a,Y55>T(+ - -)|a164>c,Y55>T(+ - -)|a166>g,I56>V(+ - +)|g176>c,S59>T(+ - +)|a190>c,S64>R(- - -)|c195>g|a196>g,N66>E(+ - -)|c198>g,N66>E(+ - -)|g226>c,V76>L(+ - +)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|g267>a|a295>t,T99>S(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| c90>g,I30>M(+ + -)|t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t| c133>t,P45>S(+ - -)|g155>t,W52>L(+ - -)|t159>a|t163>a,Y55>T(+ - -)|a164>c,Y55>T(+ - -)| a166>g,I56>V(+ - +)|g176>c,S59>T(+ - +)|a190>c,S64>R(- - -)|c195>g| a196>g,N66>E(+ - -)|c198>g,N66>E(+ - -)|g226>c,V76>L(+ - +)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|g267>a|a295>t,T99>S(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| 320 222 200 22 4 18 2 3 2 0 2 0 2 2 3 3 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 1 0 0 0 0 0 0 1 0 1 51 51 46 5 1 4 0 0 1 0 1 0 0 2 0 0 1 0 30 21 17 4 1 3 1 0 0 0 1 0 0 0 1 1 0 0 117 113 104 9 1 8 1 2 0 0 0 0 2 0 2 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatgaggaattattac tggagctggatccggcagtccccagggaagggactggagttgatagggact gtctattacactgggcgcacg gagtacaacccctccctcaagagtcgactcaccttatcactagacacgtccaagaaccagttctccctaaagctggctctgtgaccgctgcggactcggccatttattactgt gcgagagacccccgagactattgtagtggtggttactgttttgttggtgcttttgatatc ARDPRDYCSGGYCFVGAFDI IGHV4-59 IGHD2-15 IGHJ3 ggtggctccatgaggaattattac tggagctggatccggcagtccccagggaagggactggagttgatagggact gtctattacactgggcgcacg gagtacaacccctccctcaagagtcgactcaccttatcactagacacgtccaagaaccagttctccctaaagctggctctgtgaccgctgcggactcggccatttattactgt gcgagagacccccgagactattgtagtggtggttactgttttgttggtgcttttgatatc IGG1 22 209 7 15 5 13 2 9 2 2 12 6 JY8QFUQ01B68YS 2017 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 936 92.63 201/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD4-17*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARPLRATYGDYVNW in-frame + ctctggattcaccttcgataactatggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagttatatcaaaggatggaagtattgaatactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacaatttatctgcaaatgaacattgtgagggttgaggacacggctatgtattactgtgcgagaccccttcgggcaacttacggtgactacgtgaactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [287] a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)|t172>a,Y58>K(- - -)|t174>g,Y58>K(- - -)|a191>t,N64>I(- - -)|a193>g,K65>E(+ - -)|g258>a|c259>a,L87>I(+ + +)|g261>t,L87>I(+ + +)|g278>t,S93>I(- - -)|c279>t,S93>I(- - -)|c280>g,L94>V(+ - +)|a285>g|c287>t,A96>V(+ - +)|g301>a,V101>M(+ - -)| a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)| t172>a,Y58>K(- - -)|t174>g,Y58>K(- - -)|a191>t,N64>I(- - -)|a193>g,K65>E(+ - -)| g258>a|c259>a,L87>I(+ + +)|g261>t,L87>I(+ + +)|g278>t,S93>I(- - -)|c279>t,S93>I(- - -)|c280>g,L94>V(+ - +)|a285>g|c287>t,A96>V(+ - +)|g301>a,V101>M(+ - -)| g319>c|a320>c| 318 (320) 223 (225) 207 16 (18) 2 (4) 14 3 4 2 0 0 (1) 1 1 1 0 (1) 1 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 2 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 0 0 0 0 1 1 0 0 0 1 117 114 105 9 2 7 1 2 2 0 0 1 0 0 0 1 2 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcgataactatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcaaaggatggaagtattgaa tactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacaatttatctgcaaatgaacattgtgagggttgaggacacggctatgtattactgt gcgagaccccttcgggcaacttacggtgactacgtgaac ARPLRATYGDYVN IGHV3-30 IGHD4-17 IGHJ4 ggattcaccttcgataactatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcaaaggatggaagtattgaa tactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacaatttatctgcaaatgaacattgtgagggttgaggacacggctatgtattactgt gcgagaccccttcgggcaacttacggtgactacgtgaac IGA2 16 213 9 7 6 10 3 6 2 0 7 7 JY8QFUQ01B6BDJ 14245 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD3-10*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKKKHYNSGTYLYNFDYW in-frame + ctctggattcacctttagcaactttgccatgacctgggtccgccaggctccagggaggggactggagtgggtctcaactattagtggtagtgatggtagcacatacttcgcagactccgtgaagggccgattcaccatctccagggacaatttcaagaacacgctgtatctgcaaatggacagcctgagagccgaggacacggccgtatattactgtgcgaaaaaaaagcattataattcggggacttatttatacaactttgactactggggccggggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccag NA IGA2 71 95 [299, 301] g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)|g119>c,S40>T(+ - +)|a143>g,K48>R(+ + +)|g147>a|g163>a,A55>T(- - -)|g185>a,G62>D(- - -)|a200>t,Y67>F(- + -)|g225>a|a240>g|c248>t,S83>F(- - -)|a274>g,N92>D(+ + -)| g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)| g119>c,S40>T(+ - +)|a143>g,K48>R(+ + +)|g147>a|g163>a,A55>T(- - -)| g185>a,G62>D(- - -)| a200>t,Y67>F(- + -)|g225>a|a240>g|c248>t,S83>F(- - -)|a274>g,N92>D(+ + -)| g319>a| 318 (320) 223 (225) 211 (212) 12 (13) 3 (4) 9 3 5 (6) 1 0 0 0 2 0 1 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 1 0 0 0 0 0 51 51 47 4 1 3 1 2 0 0 0 0 0 0 1 0 0 0 30 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 117 114 109 5 2 3 2 1 1 0 0 0 1 0 0 0 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcaactttgcc atgacctgggtccgccaggctccagggaggggactggagtgggtctcaact attagtggtagtgatggtagcaca tacttcgcagactccgtgaagggccgattcaccatctccagggacaatttcaagaacacgctgtatctgcaaatggacagcctgagagccgaggacacggccgtatattactgt gcgaaaaaaaagcattataattcggggacttatttatacaactttgactac AKKKHYNSGTYLYNFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcacctttagcaactttgcc atgacctgggtccgccaggctccagggaggggactggagtgggtctcaact attagtggtagtgatggtagcaca tacttcgcagactccgtgaagggccgattcaccatctccagggacaatttcaagaacacgctgtatctgcaaatggacagcctgagagccgaggacacggccgtatattactgt gcgaaaaaaaagcattataattcggggacttatttatacaactttgactac IGA2 12 213 9 3 6 7 3 5 3 0 6 3 JY8QFUQ01B6CFI 22016 productive (see comment) Homsap IGHV3-9*01 F 873 89.4 194/217 nt 88.94 193/217 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [1.17.38.11] CVKDRKGSNWYYFENW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'G' (1 nucleotides at position 49 to 49 in the user submitted sequence), (cause frameshift); ctctggattcaggtttgatgattatgccatgcactgggtccggcaagcGtccagggaagggcctggagtgggtctcaagtatcagctggggtggtgctagtatcctctatgcggactccgtgaagggccgattcaccatctccagagacaatgccaggaactccctctacttgcaaatggacagtctgagacctgatgacacggccttctattactgtgtaaaagatcgaaagggcagcaattggtactactttgagaactggggccagggaaccctggtctccgtctcctcagcatccccgaccagcccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccca NA IGA2 71 95 [292, 291] c86>g,T29>R(- - -)|c87>g,T29>R(- - -)|g163>a,G55>S(+ + -)|t168>c|t171>c|a175>g,N59>G(- - -)|a176>g,N59>G(- - -)|a184>g,S62>G(+ + -)|g188>c,G63>A(- + -)|c192>t|a195>c|g196>c,G66>L(- - -)|g197>t,G66>L(- - -)|t210>c|c246>t|a251>g,K84>R(+ + +)|g261>c|t264>c|c265>t|a274>g,N92>D(+ + -)|g286>c,A96>P(- - -)|g291>t,E97>D(+ - +)|g303>c,L101>F(+ - -)|c314>t,A105>V(+ - +)| c86>g,T29>R(- - -)|c87>g,T29>R(- - -)| g163>a,G55>S(+ + -)| t168>c|t171>c|a175>g,N59>G(- - -)|a176>g,N59>G(- - -)|a184>g,S62>G(+ + -)|g188>c,G63>A(- + -)|c192>t|a195>c| g196>c,G66>L(- - -)|g197>t,G66>L(- - -)|t210>c|c246>t|a251>g,K84>R(+ + +)|g261>c|t264>c|c265>t|a274>g,N92>D(+ + -)|g286>c,A96>P(- - -)|g291>t,E97>D(+ - +)|g303>c,L101>F(+ - -)| c314>t,A105>V(+ - +)|a322>c| 321 (322) 226 (227) 202 24 (25) 9 (10) 15 5 1 4 4 1 (2) 0 0 0 5 2 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 0 0 0 2 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 16 8 4 4 3 0 1 2 1 0 0 0 1 0 0 0 117 114 102 12 5 7 2 0 2 2 0 0 0 0 4 0 2 0 9 (10) 9 8 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaggtttgatgattatgcc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt atcagctggggtggtgctagtatc ctctatgcggactccgtgaagggccgattcaccatctccagagacaatgccaggaactccctctacttgcaaatggacagtctgagacctgatgacacggccttctattactgt gtaaaagatcgaaagggcagcaattggtactactttgagaac VKDRKGSNWYYFEN IGHV3-9 IGHD5-24 IGHJ4 ggattcaggtttgatgattatgcc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt atcagctggggtggtgctagtatc ctctatgcggactccgtgaagggccgattcaccatctccagagacaatgccaggaactccctctacttgcaaatggacagtctgagacctgatgacacggccttctattactgt gtaaaagatcgaaagggcagcaattggtactactttgagaac IGA2 23 213 13 10 4 13 9 10 5 4 8 6 JY8QFUQ01B6DDX 7940 productive Homsap IGHV3-7*03 F 846 88.02 191/217 nt NA Homsap IGHJ6*03 F (see comment) 183 84.31 43/51 nt Homsap IGHD6-6*01 F 3 8 8 8 8.8.8 [1.17.38.11] CTRRGFMDVW in-frame + Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) ctccggattcatttttggtgactattggatgagctgggtccgccaggttccagggaagggcctggagtgggtggccactacaaacgaggacgaaagtgacaagcggtatgtggactctgtgaagggccgcttcaccatctccagagacaacgccaagaactcactgtatttgcaaatgaacagcctgagaggccaggacgcggccgtgtattactgtaccagaagaggattcatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaaagatgggaacgtggtcgtcgcatgcctggtcccagggcttcttccccca NA IGA2 71 95 [272, 274] t78>c|c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|a103>g,S35>G(+ + -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c134>t,A45>V(+ - +)|g147>c|a164>c,N55>T(- + -)|c165>t,N55>T(- + -)|t167>c,I56>T(- - -)|g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a174>g,Q58>E(+ + -)|t177>c|g185>a,G62>E(- - -)|g192>c,E64>D(+ - +)|a195>g|t196>c,Y66>R(- - -)|a197>g,Y66>R(- - -)|c198>g,Y66>R(- - -)|a225>c|c265>t|c287>g,A96>G(- + -)|g289>c,E97>Q(+ + -)|a295>g,T99>A(- - -)|g313>a,A105>T(- - -)|g315>c,A105>T(- - -)| t78>c| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|a103>g,S35>G(+ + -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| c134>t,A45>V(+ - +)|g147>c|a164>c,N55>T(- + -)|c165>t,N55>T(- + -)| t167>c,I56>T(- - -)|g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a174>g,Q58>E(+ + -)|t177>c|g185>a,G62>E(- - -)|g192>c,E64>D(+ - +)|a195>g| t196>c,Y66>R(- - -)|a197>g,Y66>R(- - -)|c198>g,Y66>R(- - -)|a225>c|c265>t|c287>g,A96>G(- + -)|g289>c,E97>Q(+ + -)|a295>g,T99>A(- - -)| g313>a,A105>T(- - -)|g315>c,A105>T(- - -)|g319>a|a320>g| 318 (320) 223 (225) 195 28 (30) 6 (8) 22 6 (7) 3 (4) 5 4 2 0 0 0 5 3 0 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 19 5 0 5 2 1 2 0 0 0 0 0 0 0 0 0 51 51 47 4 1 3 0 0 2 0 1 0 0 0 1 0 0 0 30 24 16 8 2 6 2 1 0 2 0 0 0 0 2 1 0 0 117 114 106 8 2 6 2 0 1 1 1 0 0 0 1 2 0 0 6 (8) 6 4 2 (4) 0 (2) 2 0 (1) 1 (2) 0 0 0 0 0 0 1 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcatttttggtgactattgg atgagctgggtccgccaggttccagggaagggcctggagtgggtggccact acaaacgaggacgaaagtgacaag cggtatgtggactctgtgaagggccgcttcaccatctccagagacaacgccaagaactcactgtatttgcaaatgaacagcctgagaggccaggacgcggccgtgtattactgt accagaagaggattcatggacgtc TRRGFMDV IGHV3-7 IGHD6-6 IGHJ6 ggattcatttttggtgactattgg atgagctgggtccgccaggttccagggaagggcctggagtgggtggccact acaaacgaggacgaaagtgacaag cggtatgtggactctgtgaagggccgcttcaccatctccagagacaacgccaagaactcactgtatttgcaaatgaacagcctgagaggccaggacgcggccgtgtattactgt accagaagaggattcatggacgtc IGA2 25 213 16 9 7 14 9 11 3 2 9 11 JY8QFUQ01B6G3Q 18689 productive Homsap IGHV4-39*02 F (see comment) 776 83.71 185/221 nt NA Homsap IGHJ6*02 F 193 79.03 49/62 nt Homsap IGHD2-15*01 F 2 10 7 19 10.7.19 [1.17.38.11] CARHLTNSDVVHHFYSGVDVW in-frame + Low V-REGION identity (83,71% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tctctggtgcctccatcagtaatgataattatttctgggcctgggtccgccagtccccagggaagggtctgcagtggattggcagtatgtattatagtgggggcaccttttacgacccgtccctcaagggtcgaatcaagttgtccgttgacgtgtcgaagagccacttcctcctgaacctgacctctgcgaccgccgcagacacggctgtctattactgtgcgaggcatttgaccaactcagatgtggtccatcacttctactccggagtagacgtctggggcctcgggaccacggtcaccgtctcctccgcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [309] g83>c,G28>A(- + -)|c93>t|g101>a,S34>N(- - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)|c111>t|a113>t,Y38>F(- + -)|g119>c,G40>A(- + -)|a124>g,I42>V(+ - +)|c133>t,P45>S(+ - -)|g147>t|g151>c,E51>Q(+ + -)|g162>c|c168>g,I56>M(+ + -)|a190>g,S64>G(+ + -)|a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|a202>g,N68>D(+ + -)|a220>g,S74>G(+ + -)|g226>a,V76>I(+ - +)|c230>a,T77>K(- - -)|c231>g,T77>K(- - -)|a232>t,I78>L(+ + +)|a234>g,I78>L(+ + +)|a240>t|a244>g,T82>V(- - -)|c245>t,T82>V(- - -)|c249>g|a254>g,N85>S(- - -)|t262>c,S88>L(- - -)|c263>t,S88>L(- - -)|g270>c,K90>N(+ - -)|g275>c,S92>T(+ - +)|t281>c,V94>A(+ - +)|g303>c| g83>c,G28>A(- + -)|c93>t|g101>a,S34>N(- - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)|c111>t|a113>t,Y38>F(- + -)| g119>c,G40>A(- + -)|a124>g,I42>V(+ - +)|c133>t,P45>S(+ - -)|g147>t|g151>c,E51>Q(+ + -)|g162>c| c168>g,I56>M(+ + -)|a190>g,S64>G(+ + -)| a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|a202>g,N68>D(+ + -)|a220>g,S74>G(+ + -)|g226>a,V76>I(+ - +)|c230>a,T77>K(- - -)|c231>g,T77>K(- - -)|a232>t,I78>L(+ + +)|a234>g,I78>L(+ + +)|a240>t|a244>g,T82>V(- - -)|c245>t,T82>V(- - -)|c249>g|a254>g,N85>S(- - -)|t262>c,S88>L(- - -)|c263>t,S88>L(- - -)|g270>c,K90>N(+ - -)|g275>c,S92>T(+ - +)|t281>c,V94>A(+ - +)|g303>c| a318>g|g319>c| 317 (320) 226 (229) 190 (191) 36 (38) 7 (9) 29 8 (9) 4 6 2 0 1 4 0 7 (8) 3 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 22 8 2 6 1 3 2 0 0 0 1 0 1 0 0 0 51 51 45 6 2 4 1 0 1 0 0 0 0 0 3 0 1 0 30 21 19 2 0 2 1 0 0 0 0 0 0 0 0 1 0 0 117 114 94 20 3 17 5 1 3 2 0 1 3 0 3 2 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgcctccatcagtaatgataattatttc tgggcctgggtccgccagtccccagggaagggtctgcagtggattggcagt atgtattatagtgggggcacc ttttacgacccgtccctcaagggtcgaatcaagttgtccgttgacgtgtcgaagagccacttcctcctgaacctgacctctgcgaccgccgcagacacggctgtctattactgt gcgaggcatttgaccaactcagatgtggtccatcacttctactccggagtagacgtc ARHLTNSDVVHHFYSGVDV IGHV4-39 IGHD2-15 IGHJ6 ggtgcctccatcagtaatgataattatttc tgggcctgggtccgccagtccccagggaagggtctgcagtggattggcagt atgtattatagtgggggcacc ttttacgacccgtccctcaagggtcgaatcaagttgtccgttgacgtgtcgaagagccacttcctcctgaacctgacctctgcgaccgccgcagacacggctgtctattactgt gcgaggcatttgaccaactcagatgtggtccatcacttctactccggagtagacgtc IGA1 36 216 20 16 10 22 10 14 5 2 21 8 JY8QFUQ01B6LHF 19170 productive Homsap IGHV3-48*01 F 811 85.84 188/219 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [2.17.38.11] CATDRLRGDKYNFDDW in-frame - ggctacggatttgtcttcagtggcactaatatgaattggctccgccaggctccagggaaggggctggagtggatttcacacattagtcacaatagtgaaaccatattctatgcagactctgtgaggggccgagtcaccatctccagagacaatgccaggaactcactgtatctgcacatgagcagcctgagagccgaggacacggcagtatattactgtgcgaccgaccgccttcgtggagataaatacaattttgacgactggggccagggaaccctggtcaccgtctccccagcatcccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [293] c74>g,A25>G(- + -)|c77>a,S26>Y(+ - -)|t78>c,S26>Y(+ - -)|c84>t|a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|a106>g,S36>G(+ + -)|t109>a,Y37>T(+ - -)|a110>c,Y37>T(+ - -)|g113>a,S38>N(- - -)|c114>t,S38>N(- - -)|c120>t|g124>c,V42>L(+ - +)|g157>a,V53>I(+ - +)|t163>c,Y55>H(+ - -)|a172>c,S58>H(+ - -)|g173>a,S58>H(+ - -)|t174>c,S58>H(+ - -)|g176>a,S59>N(- - -)|a187>g,S63>E(- - -)|g188>a,S63>E(- - -)|t189>a,S63>E(- - -)|a197>t,Y66>F(- + -)|c201>t|a215>g,K72>R(+ + +)|t226>g,F76>V(+ - -)|a251>g,K84>R(+ + +)|a270>c,Q90>H(- + -)|a275>g,N92>S(- - -)|t300>a|g303>a| c74>g,A25>G(- + -)|c77>a,S26>Y(+ - -)|t78>c,S26>Y(+ - -)| c84>t|a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|a106>g,S36>G(+ + -)|t109>a,Y37>T(+ - -)|a110>c,Y37>T(+ - -)|g113>a,S38>N(- - -)|c114>t,S38>N(- - -)| c120>t|g124>c,V42>L(+ - +)|g157>a,V53>I(+ - +)|t163>c,Y55>H(+ - -)| a172>c,S58>H(+ - -)|g173>a,S58>H(+ - -)|t174>c,S58>H(+ - -)|g176>a,S59>N(- - -)|a187>g,S63>E(- - -)|g188>a,S63>E(- - -)|t189>a,S63>E(- - -)| a197>t,Y66>F(- + -)|c201>t|a215>g,K72>R(+ + +)|t226>g,F76>V(+ - -)|a251>g,K84>R(+ + +)|a270>c,Q90>H(- + -)|a275>g,N92>S(- - -)|t300>a|g303>a| g317>c,R106>T(- - -)|a318>c,R106>T(- - -)| 316 (320) 223 (227) 192 (194) 31 (33) 5 26 (28) 6 6 5 3 3 (4) 1 1 3 1 (2) 1 0 1 78 6 3 3 0 3 0 0 0 1 0 1 0 0 0 1 0 0 36 24 16 8 1 7 2 1 3 0 1 0 0 1 0 0 0 0 51 51 47 4 1 3 0 1 1 1 0 0 0 0 1 0 0 0 30 24 17 7 0 7 1 3 0 1 1 0 0 1 0 0 0 0 117 114 105 9 3 6 3 1 1 0 1 0 1 1 0 0 0 1 4 (8) 4 4 (6) 0 (2) 0 0 (2) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ggctac ggatttgtcttcagtggcactaat atgaattggctccgccaggctccagggaaggggctggagtggatttcacac attagtcacaatagtgaaaccata ttctatgcagactctgtgaggggccgagtcaccatctccagagacaatgccaggaactcactgtatctgcacatgagcagcctgagagccgaggacacggcagtatattactgt gcgaccgaccgccttcgtggagataaatacaattttgacgac ATDRLRGDKYNFDD IGHV3-48 IGHD5-24 IGHJ4 ggatttgtcttcagtggcactaat atgaattggctccgccaggctccagggaaggggctggagtggatttcacac attagtcacaatagtgaaaccata ttctatgcagactctgtgaggggccgagtcaccatctccagagacaatgccaggaactcactgtatctgcacatgagcagcctgagagccgaggacacggcagtatattactgt gcgaccgaccgccttcgtggagataaatacaattttgacgac IGA1 28 213 19 9 11 12 8 16 4 1 9 14 JY8QFUQ01B6LVM 9286 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 873 89.4 194/217 nt NA Homsap IGHJ3*02 F (see comment) 205 90 45/50 nt Homsap IGHD6-25*01 F 3 8 8 15 8.8.15 [1.17.38.11] CVRGGRGQQREDAFDIW in-frame - Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) ctctggattcatcttcagtagttatagtttgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattgatatgagtagttcttacatctattatggagactcagtgaggggccgatttaccatctccagagacaacgccgagagctcactgtatctgcagatgagcagcctgagagccgaggacacggctttatattattgtgtgagaggcgggagggggcagcagcgagaagatgcttttgacatttggggccaagggacaacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [293] c86>t,T29>I(- - -)|c108>t|c114>t|a115>t,M39>L(+ + -)|a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|g173>t,S58>M(- - -)|t174>g,S58>M(- - -)|a187>t|g188>c|a195>c|c198>t|c201>t|c203>g,A68>G(- + -)|a215>g,K72>R(+ + +)|c228>t|a250>g,K84>E(+ - -)|a254>g,N85>S(- - -)|a270>g|a275>g,N92>S(- - -)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|c309>t|c314>t,A105>V(+ - +)| c86>t,T29>I(- - -)|c108>t|c114>t| a115>t,M39>L(+ + -)| a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|g173>t,S58>M(- - -)|t174>g,S58>M(- - -)|a187>t|g188>c|a195>c| c198>t|c201>t|c203>g,A68>G(- + -)|a215>g,K72>R(+ + +)|c228>t|a250>g,K84>E(+ - -)|a254>g,N85>S(- - -)|a270>g|a275>g,N92>S(- - -)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|c309>t| c314>t,A105>V(+ - +)|a320>g| 319 (320) 224 (225) 200 24 (25) 10 (11) 14 6 (7) 2 8 0 1 0 2 0 1 1 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 3 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 30 24 17 7 3 4 1 1 0 0 1 0 1 0 1 0 1 1 117 114 102 12 5 7 5 1 4 0 0 0 0 0 0 1 1 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagtagttatagt ttgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attgatatgagtagttcttacatc tattatggagactcagtgaggggccgatttaccatctccagagacaacgccgagagctcactgtatctgcagatgagcagcctgagagccgaggacacggctttatattattgt gtgagaggcgggagggggcagcagcgagaagatgcttttgacatt VRGGRGQQREDAFDI IGHV3-21 IGHD6-25 IGHJ3 ggattcatcttcagtagttatagt ttgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attgatatgagtagttcttacatc tattatggagactcagtgaggggccgatttaccatctccagagacaacgccgagagctcactgtatctgcagatgagcagcctgagagccgaggacacggctttatattattgt gtgagaggcgggagggggcagcagcgagaagatgcttttgacatt IGA1 23 213 15 8 9 13 6 10 5 5 8 5 JY8QFUQ01B6UYE 21114 productive Homsap IGHV3-7*01 F 941 92.66 202/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-2*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSAFFSTGYW in-frame + gctctagattcacctttaggacatattggatgagttgggtccgccaagctccagggaaggggctggagtgggtggccaacataaaggaagatggaagtgagatacactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtttctccaaatgaacagcttgagagtcgatgacacggctgtgtattactgtgcgatcagtagtgccttcttctccacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [282] c74>g|g79>a,G27>R(- - -)|t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|c120>t|g132>a|c172>g,Q58>E(+ + -)|a194>t,K65>I(- + -)|t196>c,Y66>H(+ - -)|t256>a,S86>T(+ - +)|a263>t,Y88>F(- + -)|g267>c|c280>t|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)| c74>g| g79>a,G27>R(- - -)|t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)| c120>t|g132>a| c172>g,Q58>E(+ + -)|a194>t,K65>I(- + -)| t196>c,Y66>H(+ - -)|t256>a,S86>T(+ - +)|a263>t,Y88>F(- + -)|g267>c|c280>t|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)| g317>t,R106>I(- + -)|a318>c,R106>I(- + -)|g319>a|a320>g| 316 (320) 222 (226) 206 16 (20) 5 (7) 11 (13) 0 (1) 2 (3) 3 1 0 (1) 1 2 1 2 2 1 (2) 1 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 20 4 0 4 0 1 0 0 0 1 0 0 1 0 0 1 51 51 49 2 2 0 0 1 1 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 117 114 107 7 2 5 0 0 2 1 0 0 1 1 1 0 1 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gctct agattcacctttaggacatattgg atgagttgggtccgccaagctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagata cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtttctccaaatgaacagcttgagagtcgatgacacggctgtgtattactgt gcgatcagtagtgccttcttctccacgggctac AISSAFFSTGY IGHV3-7 IGHD2-2 IGHJ4 agattcacctttaggacatattgg atgagttgggtccgccaagctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagata cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtttctccaaatgaacagcttgagagtcgatgacacggctgtgtattactgt gcgatcagtagtgccttcttctccacgggctac IGA2 15 213 6 9 5 10 1 5 4 0 5 6 JY8QFUQ01B70J3 12715 productive Homsap IGHV1-24*01 F 970 95.31 203/213 nt NA Homsap IGHJ5*02 F 141 77.08 37/48 nt Homsap IGHD5-18*01 F 1 8 8 7 8.8.7 [X.17.38.11] CAIGAMATW in-frame + cggaaaaccctcactgaagtatccatgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggaggatttgatcctgaagatggtgaaataatctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgtgcaataggagctatggccacctggggccagggaaccctggtcaccgtcgactcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [265] g79>c,G27>R(- - -)|a81>g,G27>R(- - -)|t82>a,Y28>K(- - -)|c84>a,Y28>K(- - -)|t109>g,L37>V(+ - +)|t165>a|c194>t,T65>I(- - -)|g226>a,V76>I(+ - +)|a232>g,M78>V(+ - -)|g291>a|c317>t,T106>I(- - -)| g79>c,G27>R(- - -)|a81>g,G27>R(- - -)|t82>a,Y28>K(- - -)|c84>a,Y28>K(- - -)|t109>g,L37>V(+ - +)| t165>a| c194>t,T65>I(- - -)| g226>a,V76>I(+ - +)|a232>g,M78>V(+ - -)|g291>a| c317>t,T106>I(- - -)|a320>g| 319 (320) 220 (221) 209 11 (12) 2 (3) 9 2 (3) 2 2 0 0 1 0 2 1 0 0 1 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 0 0 0 0 1 0 1 1 0 0 1 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 117 114 111 3 1 2 1 2 0 0 0 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,14-15(FR1)|aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|aa,107-108(CDR1)|ta,110-111(CDR1)|aa,141-142(FR2)|aa,143-144(FR2)|aa,176-177(CDR2)|aa,191-192(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,249-250(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,14-15(FR1)|tt,74-75(FR1)|ta,82-83(CDR1)|tt,109-110(CDR1)|tt,149-150(FR2)|tt,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,249-250(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggta,12-15(FR1)|ggct,24-27(FR1)|ggtt,72-75(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,163-166(FR2-CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| cggaaaaccctcactgaagtatcc atgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggagga tttgatcctgaagatggtgaaata atctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcaataggagctatggccacc AIGAMAT IGHV1-24 IGHD5-18 IGHJ5 cggaaaaccctcactgaagtatcc atgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggagga tttgatcctgaagatggtgaaata atctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcaataggagctatggccacc IGA1 10 213 5 5 3 5 2 5 2 0 2 6 JY8QFUQ01B71ED 15843 productive Homsap IGHV3-9*01 F 802 85.39 187/219 nt NA Homsap IGHJ6*02 F (see comment) 184 77.42 48/62 nt Homsap IGHD3-9*01 F 2 8 8 16 8.8.16 [2.17.38.11] CVKDLSGRPKDMHGMDVW in-frame - Other possibilities: Homsap_IGHJ4*01 (shorter alignment but highest percentage of identity) gcctctgggttcagttttagcaattatgccatgcactgggtccgtcaagctccagggaagggcctgcaatgggtctcacgtattaattggaacagtggaaacatagcctatgcggcctctgtgaggggccgattcaccgtctccagagacgacgccgagcactccctgtatttacaaatgaatggtctgacaactgaagacacggccttatactattgtgtgaaagatttgtctggccgaccaaaggacatgcacggcatggacgtctggggccaagggaccacggtcaccgtcgcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [298] a81>g|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g103>a,D35>S(- - -)|a104>g,D35>S(- - -)|t105>c,D35>S(- - -)|g106>a,D36>N(+ + -)|g129>t|g151>c,E51>Q(+ + -)|g153>a,E51>Q(+ + -)|g163>c,G55>R(- - -)|g170>a,S57>N(- - -)|t177>c|t189>a|g191>a,S64>N(- - -)|g197>c,G66>A(- + -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|a244>g,N82>D(+ + -)|a250>g,K84>E(+ - -)|a253>c,N85>H(- - -)|c265>t|g267>a|c276>t|a277>g,S93>G(+ + -)|g284>c,R95>T(- - -)|g286>a,A96>T(- - -)|g291>a|g303>a|t306>c|c309>t|c314>t,A105>V(+ - +)|a315>g,A105>V(+ - +)| a81>g|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g103>a,D35>S(- - -)|a104>g,D35>S(- - -)|t105>c,D35>S(- - -)|g106>a,D36>N(+ + -)| g129>t|g151>c,E51>Q(+ + -)|g153>a,E51>Q(+ + -)|g163>c,G55>R(- - -)| g170>a,S57>N(- - -)|t177>c|t189>a|g191>a,S64>N(- - -)| g197>c,G66>A(- + -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|a244>g,N82>D(+ + -)|a250>g,K84>E(+ - -)|a253>c,N85>H(- - -)|c265>t|g267>a|c276>t|a277>g,S93>G(+ + -)|g284>c,R95>T(- - -)|g286>a,A96>T(- - -)|g291>a|g303>a|t306>c|c309>t| c314>t,A105>V(+ - +)|a315>g,A105>V(+ - +)|a322>t| 321 (322) 228 (229) 194 34 (35) 11 (12) 23 8 9 5 3 2 0 0 (1) 1 4 1 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 1 6 2 2 1 1 0 0 0 0 0 1 0 0 51 51 47 4 1 3 0 1 0 0 0 0 0 0 2 0 1 0 30 24 20 4 2 2 0 2 0 1 0 0 0 1 0 0 0 0 117 114 97 17 7 10 5 4 3 1 2 0 0 0 2 0 0 0 9 (10) 9 7 2 (3) 0 (1) 2 1 0 1 0 0 0 0 (1) 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| gcctct gggttcagttttagcaattatgcc atgcactgggtccgtcaagctccagggaagggcctgcaatgggtctcacgt attaattggaacagtggaaacata gcctatgcggcctctgtgaggggccgattcaccgtctccagagacgacgccgagcactccctgtatttacaaatgaatggtctgacaactgaagacacggccttatactattgt gtgaaagatttgtctggccgaccaaaggacatgcacggcatggacgtc VKDLSGRPKDMHGMDV IGHV3-9 IGHD3-9 IGHJ6 gggttcagttttagcaattatgcc atgcactgggtccgtcaagctccagggaagggcctgcaatgggtctcacgt attaattggaacagtggaaacata gcctatgcggcctctgtgaggggccgattcaccgtctccagagacgacgccgagcactccctgtatttacaaatgaatggtctgacaactgaagacacggccttatactattgt gtgaaagatttgtctggccgaccaaaggacatgcacggcatggacgtc IGA1 32 213 23 9 13 19 10 13 8 3 13 8 JY8QFUQ01B757D 17390 productive Homsap IGHV4-61*02 F (see comment) 569 73.3 162/221 nt NA Homsap IGHJ4*02 F (see comment) 141 77.08 37/48 nt Homsap IGHD6-6*01 F 1 10 7 16 10.7.16 [1.17.38.11] CARECSNSKVGPRALDSW in-frame + Low V-REGION identity (73,30% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ5*01 (highest number of consecutive identical nucleotides) tttctggtggcccgatcaaaagtcctgattaccattggacgtggatccggcaggccgccgggaaggggctggagtgggtcgggcgtgtctatatgactggctatgtcgagaacaatccatccctctccgggcgtctctccatgtcgattgacacggcgaagaatcagttttctatgacattgacttctgtgaccgccgcagacacggccctttatttttgtgcgagggagtgtagtaattcgaaagtcggaccgagagcgcttgactcctggggcccgggaagtctggtcaccgtatcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [300] c75>t|t85>c,S29>P(+ - -)|c87>g,S29>P(+ - -)|g92>a,S31>K(- - -)|c93>a,S31>K(- - -)|g103>c,G35>P(+ - -)|g104>c,G35>P(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t112>c,Y38>H(+ - -)|c114>t,Y38>H(+ - -)|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>g,P45>A(- - -)|a147>g|a157>g,I53>V(+ - +)|t159>c,I53>V(+ - +)|a166>g,I56>V(+ - +)|c173>t,T58>M(- - -)|c174>g,T58>M(- - -)|g176>c,S59>T(+ - +)|g189>c|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|c192>t,S64>Y(+ - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|a196>g,N66>E(+ - -)|c198>g,N66>E(+ - -)|t199>a,Y67>N(- - -)|c204>t|c207>a|a214>t,K72>S(- - -)|a215>c,K72>S(- - -)|g216>c,K72>S(- - -)|a220>g,S74>G(+ + -)|t222>g,S74>G(+ + -)|a225>t|g226>c,V76>L(+ - +)|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|a237>g|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|t247>g,S83>A(- + -)|c249>g,S83>A(- + -)|c255>t|c261>t|c264>t|c265>a,L89>M(+ + -)|a269>c,K90>T(- - -)|g270>a,K90>T(- - -)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)|a318>g| c75>t| t85>c,S29>P(+ - -)|c87>g,S29>P(+ - -)|g92>a,S31>K(- - -)|c93>a,S31>K(- - -)|g103>c,G35>P(+ - -)|g104>c,G35>P(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t112>c,Y38>H(+ - -)|c114>t,Y38>H(+ - -)| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>g,P45>A(- - -)|a147>g|a157>g,I53>V(+ - +)|t159>c,I53>V(+ - +)| a166>g,I56>V(+ - +)|c173>t,T58>M(- - -)|c174>g,T58>M(- - -)|g176>c,S59>T(+ - +)|g189>c|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|c192>t,S64>Y(+ - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)| a196>g,N66>E(+ - -)|c198>g,N66>E(+ - -)|t199>a,Y67>N(- - -)|c204>t|c207>a|a214>t,K72>S(- - -)|a215>c,K72>S(- - -)|g216>c,K72>S(- - -)|a220>g,S74>G(+ + -)|t222>g,S74>G(+ + -)|a225>t|g226>c,V76>L(+ - +)|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|a237>g|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|t247>g,S83>A(- + -)|c249>g,S83>A(- + -)|c255>t|c261>t|c264>t|c265>a,L89>M(+ + -)|a269>c,K90>T(- - -)|g270>a,K90>T(- - -)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| a318>g| 320 229 169 60 12 48 10 5 12 3 2 3 6 1 9 6 1 2 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 30 20 10 0 10 1 2 1 2 0 1 0 0 2 1 0 0 51 51 45 6 1 5 2 0 0 1 0 0 0 0 1 2 0 0 30 21 11 10 1 9 2 1 3 0 0 0 1 0 2 1 0 0 117 114 82 32 8 24 4 2 7 0 2 2 5 1 4 2 1 2 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tttct ggtggcccgatcaaaagtcctgattaccat tggacgtggatccggcaggccgccgggaaggggctggagtgggtcgggcgt gtctatatgactggctatgtc gagaacaatccatccctctccgggcgtctctccatgtcgattgacacggcgaagaatcagttttctatgacattgacttctgtgaccgccgcagacacggccctttatttttgt gcgagggagtgtagtaattcgaaagtcggaccgagagcgcttgactcc ARECSNSKVGPRALDS IGHV4-61 IGHD6-6 IGHJ4 ggtggcccgatcaaaagtcctgattaccat tggacgtggatccggcaggccgccgggaaggggctggagtgggtcgggcgt gtctatatgactggctatgtc gagaacaatccatccctctccgggcgtctctccatgtcgattgacacggcgaagaatcagttttctatgacattgacttctgtgaccgccgcagacacggccctttatttttgt gcgagggagtgtagtaattcgaaagtcggaccgagagcgcttgactcc IGA2 58 216 28 30 16 35 12 23 9 1 29 19 JY8QFUQ01B791I 15421 productive Homsap IGHV1-3*01 F 944 93.49 201/215 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-22*01 F 2 8 8 14 8.8.14 [0.17.38.11] CARGRYDSSGRGHEYW in-frame - ctggatacaccttcaccagtttcagtatccattgggtgcgccaggcccccggacaaaggcttgagtggatgggatggatcaacggtggcaatggtaacacaaaatattcacagaagttccaggacagactcaccattactagagacacatccgcgagcacagcctacatggacctgagcagcctgagatctgaagacacggctgtctattactgtgcgagagggcgctatgatagtagtggtcgtggccatgagtactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [288] t105>c|c108>t|a110>t,Y37>F(- + -)|t111>c,Y37>F(- + -)|g112>a,A38>S(- + -)|c113>g,A38>S(- + -)|g117>c,M39>I(+ + -)|c173>g,A58>G(- + -)|g221>a,G74>D(- - -)|g226>c,V76>L(+ - +)|c237>t|g240>a|g270>c,E90>D(+ - +)|g303>c| t105>c|c108>t|a110>t,Y37>F(- + -)|t111>c,Y37>F(- + -)|g112>a,A38>S(- + -)|c113>g,A38>S(- + -)| g117>c,M39>I(+ + -)| c173>g,A58>G(- + -)| g221>a,G74>D(- - -)|g226>c,V76>L(+ - +)|c237>t|g240>a|g270>c,E90>D(+ - +)|g303>c| a320>g| 319 (320) 222 (223) 208 14 (15) 5 (6) 9 0 (1) 3 2 2 0 0 1 0 4 2 0 0 78 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 2 4 0 1 1 2 0 0 1 0 0 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 117 114 108 6 3 3 0 2 1 0 0 0 0 0 3 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|ta,262-263(FR3)|aa,290-291(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,11-12(FR1)|tt,62-63(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,120-121(FR2)|tt,149-150(FR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,201-202(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ct ggatacaccttcaccagtttcagt atccattgggtgcgccaggcccccggacaaaggcttgagtggatgggatgg atcaacggtggcaatggtaacaca aaatattcacagaagttccaggacagactcaccattactagagacacatccgcgagcacagcctacatggacctgagcagcctgagatctgaagacacggctgtctattactgt gcgagagggcgctatgatagtagtggtcgtggccatgagtac ARGRYDSSGRGHEY IGHV1-3 IGHD3-22 IGHJ4 ggatacaccttcaccagtttcagt atccattgggtgcgccaggcccccggacaaaggcttgagtggatgggatgg atcaacggtggcaatggtaacaca aaatattcacagaagttccaggacagactcaccattactagagacacatccgcgagcacagcctacatggacctgagcagcctgagatctgaagacacggctgtctattactgt gcgagagggcgctatgatagtagtggtcgtggccatgagtac IGA1 14 213 7 7 5 11 2 3 3 2 4 5 JY8QFUQ01B7921 18874 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 941 92.66 202/218 nt 92.2 201/218 nt Homsap IGHJ5*02 F 201 88.24 45/51 nt Homsap IGHD6-13*01 F 2 8 8 16 8.8.16 [1.17.38.11] CVRGRIVSVGMAGWFDPW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 35 of V-REGION: 'G' (1 nucleotides at position 19 to 19 in the user submitted sequence), (cause frameshift); tctctggattcaccttcaGggaactattggatgcactgggtccgccaagctccagggaaggggctggagtgggtctcacgtatcaatggcgacggaagtagcacaagctacgcggactccgtgaagggccgattcaccatctccagagacaacgccgagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacagccgtatatttttgtgtaagaggccgtatagtgtcagttggtatggcaggctggttcgacccctggggccagggaacgcaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [298] c74>t|t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t|t152>a,V51>E(- + -)|t168>c|a172>g,S58>G(+ + -)|t174>c,S58>G(+ + -)|t177>c|g186>a|a250>g,K84>E(+ - -)|g297>a|t300>c|g303>a|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)|c314>t,A105>V(+ - +)| c74>t| t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t| t152>a,V51>E(- + -)| t168>c|a172>g,S58>G(+ + -)|t174>c,S58>G(+ + -)|t177>c|g186>a| a250>g,K84>E(+ - -)|g297>a|t300>c|g303>a|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| c314>t,A105>V(+ - +)|a320>g| 319 (320) 225 (226) 208 17 (18) 8 (9) 9 2 (3) 4 4 4 0 0 1 1 0 0 0 1 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 0 0 0 0 0 0 0 0 1 51 51 50 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 30 24 19 5 3 2 1 1 0 3 0 0 0 0 0 0 0 0 117 114 108 6 3 3 1 2 1 1 0 0 1 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tctct ggattcaccttcaggaactattgg atgcactgggtccgccaagctccagggaaggggctggagtgggtctcacgt atcaatggcgacggaagtagcaca agctacgcggactccgtgaagggccgattcaccatctccagagacaacgccgagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacagccgtatatttttgt gtaagaggccgtatagtgtcagttggtatggcaggctggttcgacccc VRGRIVSVGMAGWFDP IGHV3-74 IGHD6-13 IGHJ5 ggattcaccttcaggaactattgg atgcactgggtccgccaagctccagggaaggggctggagtgggtctcacgt atcaatggcgacggaagtagcaca agctacgcggactccgtgaagggccgattcaccatctccagagacaacgccgagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacagccgtatatttttgt gtaagaggccgtatagtgtcagttggtatggcaggctggttcgacccc IGA1 15 213 12 3 6 6 6 9 3 4 4 4 JY8QFUQ01B7B1B 8528 productive Homsap IGHV3-7*01 F 1017 96.77 210/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSGFYSTGYW in-frame + ctctggattcacctttagtacctattggatgggctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaaacaagatggaagtgacaaatactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtctattactgtgcgattagtagtggtttttactcgacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [281] g107>c,S36>T(+ - +)|a118>g,S40>G(+ + -)|g171>a|g192>c,E64>D(+ - +)|c248>g,A83>G(- + -)|c265>t|g303>c| g107>c,S36>T(+ - +)| a118>g,S40>G(+ + -)| g171>a|g192>c,E64>D(+ - +)| c248>g,A83>G(- + -)|c265>t|g303>c| g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 316 (320) 221 (225) 214 7 (11) 3 (5) 4 (6) 1 (2) 1 (2) 1 0 0 0 0 (1) 0 3 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 50 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 117 114 111 3 2 1 0 0 1 0 0 0 0 0 1 1 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaacaagatggaagtgacaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgattagtagtggtttttactcgacgggctac AISSGFYSTGY IGHV3-7 IGHD3-22 IGHJ4 ggattcacctttagtacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaacaagatggaagtgacaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgattagtagtggtttttactcgacgggctac IGA1 7 213 3 4 2 6 1 1 2 1 2 2 JY8QFUQ01B7DZ7 12565 productive Homsap IGHV3-33*03 F 959 93.58 204/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-18*01 F 1 8 8 13 8.8.13 [1.17.38.11] CARSDLSMVRDLDYW in-frame - cgtctggattcaccttcagtacctatggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatgatttggtatgatggaagccttacatattatgaagactccgtgaagggccgattcaccatctccagagacaactccaagaacacgctgtttctgcaaatgaatagcctgagagtcgaggacacggctctgtattactgtgcgagatccgacctctctatggttagagatcttgactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [288, 287] g107>c,S36>T(+ - +)|g163>a,V55>M(+ - -)|t165>g,V55>M(+ - -)|a168>t|t189>c|a190>c,N64>L(- - -)|a191>t,N64>L(- - -)|a194>c,K65>T(- - -)|c198>t|c203>a,A68>E(- - -)|a263>t,Y88>F(- + -)|c276>t|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)| g107>c,S36>T(+ - +)| g163>a,V55>M(+ - -)|t165>g,V55>M(+ - -)| a168>t|t189>c|a190>c,N64>L(- - -)|a191>t,N64>L(- - -)|a194>c,K65>T(- - -)| c198>t|c203>a,A68>E(- - -)|a263>t,Y88>F(- + -)|c276>t|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)| a317>g,K106>R(+ + +)|g319>t|a320>c| 316 (320) 222 (226) 208 (209) 14 (17) 4 (6) 10 (11) 0 (1) 1 3 1 2 (3) 1 3 0 2 0 0 (1) 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 30 24 19 5 2 3 0 0 0 1 2 0 2 0 0 0 0 0 117 114 108 6 2 4 0 0 3 0 0 1 1 0 1 0 0 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|aact,244-247(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cgtct ggattcaccttcagtacctatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatg atttggtatgatggaagccttaca tattatgaagactccgtgaagggccgattcaccatctccagagacaactccaagaacacgctgtttctgcaaatgaatagcctgagagtcgaggacacggctctgtattactgt gcgagatccgacctctctatggttagagatcttgactat ARSDLSMVRDLDY IGHV3-33 IGHD5-18 IGHJ4 ggattcaccttcagtacctatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatg atttggtatgatggaagccttaca tattatgaagactccgtgaagggccgattcaccatctccagagacaactccaagaacacgctgtttctgcaaatgaatagcctgagagtcgaggacacggctctgtattactgt gcgagatccgacctctctatggttagagatcttgactat IGA2 14 213 5 9 4 7 1 7 2 2 6 4 JY8QFUQ01B7GYB 21240 productive (see comment) Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 900 90.32 196/217 nt 89.86 195/217 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-13*01 F 1 8 8 15 8.8.15 [1.17.38.11] CNTDLGGSWYTFYSDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 59 of V-REGION: 6 nucleotides (from position 89 in the user submitted sequence), (do not cause frameshift) ctctggatacactgtcataaatgcctggatgagttgggtccgccaggctccagggaaggggctggagtgggttggccgtattaaaggtgatggtgtgacaacagaatacgctgcatccgtgaaaggcagattcaccatcacaagagatgactcaaggaacacgttgtatctacaaatgaacagcctgaaaaccgaggacacagccatatattattgcaacacagatctgggcgggagttggtacaccttctattctgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [293, 292] t83>a,F28>Y(- + -)|t88>g,F30>V(+ - -)|g104>t,S35>I(- - -)|t105>a,S35>I(- - -)|c108>t|c120>t|a172>g,S58>G(+ + -)|c174>t,S58>G(+ + -)|g188>t,G63>V(- - -)|c198>a,D66>E(+ - +)|c208>t,P70>S(+ - -)|t235>a,S79>T(+ - +)|t246>c|a251>g,K84>R(+ + +)|a252>g,K84>R(+ + +)|c259>t|g267>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t|t312>c|c314>a,T105>N(- + -)| t83>a,F28>Y(- + -)|t88>g,F30>V(+ - -)|g104>t,S35>I(- - -)|t105>a,S35>I(- - -)|c108>t| c120>t| a172>g,S58>G(+ + -)|c174>t,S58>G(+ + -)|g188>t,G63>V(- - -)| c198>a,D66>E(+ - +)|c208>t,P70>S(+ - -)|t235>a,S79>T(+ - +)|t246>c|a251>g,K84>R(+ + +)|a252>g,K84>R(+ + +)|c259>t|g267>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t|t312>c| c314>a,T105>N(- + -)| 320 225 203 22 7 15 3 3 6 2 0 2 0 3 0 0 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 0 1 0 0 0 0 2 0 0 1 1 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 1 0 0 0 0 0 0 0 1 0 117 114 102 12 5 7 2 3 3 2 0 1 0 1 0 0 0 0 8 8 7 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| ctct ggatacactgtcataaatgcctgg atgagttgggtccgccaggctccagggaaggggctggagtgggttggccgt attaaaggtgatggtgtgacaaca gaatacgctgcatccgtgaaaggcagattcaccatcacaagagatgactcaaggaacacgttgtatctacaaatgaacagcctgaaaaccgaggacacagccatatattattgc aacacagatctgggcgggagttggtacaccttctattctgactac NTDLGGSWYTFYSDY IGHV3-15 IGHD6-13 IGHJ4 ggatacactgtcataaatgcctgg atgagttgggtccgccaggctccagggaaggggctggagtgggttggccgt attaaaggtgatggtgtgacaaca gaatacgctgcatccgtgaaaggcagattcaccatcacaagagatgactcaaggaacacgttgtatctacaaatgaacagcctgaaaaccgaggacacagccatatattattgc aacacagatctgggcgggagttggtacaccttctattctgactac IGA1 21 213 14 7 9 12 5 9 6 1 7 7 JY8QFUQ01B7L1I 15052 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 1035 97.7 212/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD6-25*01 F 1 8 8 13 8.8.13 [1.17.38.11] CARDLTGYSSERNYW in-frame - ctctggattcaccttcagtaactatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattagtagtagtgctaggtacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgcgagacctgaccgggtatagcagcgaacgaaactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] g107>a,S36>N(- - -)|a184>g,S62>A(- + -)|g185>c,S62>A(- + -)|t189>g,S63>R(- - -)|a263>c,Y88>S(+ - -)|a316>c| g107>a,S36>N(- - -)| a184>g,S62>A(- + -)|g185>c,S62>A(- + -)|t189>g,S63>R(- - -)| a263>c,Y88>S(+ - -)| a316>c| 320 225 219 6 1 5 1 1 0 0 2 0 0 0 1 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 1 0 0 1 117 114 113 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 8 8 7 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtaactatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtgctaggtacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgcgagacctgaccgggtatagcagcgaacgaaactac ARDLTGYSSERNY IGHV3-21 IGHD6-25 IGHJ4 ggattcaccttcagtaactatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtgctaggtacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgcgagacctgaccgggtatagcagcgaacgaaactac IGA1 5 213 2 3 1 2 1 3 0 0 1 4 JY8QFUQ01B7Q12 18936 productive Homsap IGHV3-30*01 F, or Homsap IGHV3-30*11 F 962 94.42 203/215 nt NA Homsap IGHJ6*02 F (see comment) 202 80.65 50/62 nt Homsap IGHD2-2*01 F 2 8 8 14 8.8.14 [0.17.38.11] CVREGRGTAAYGLDVW in-frame - Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) ctggattcaccttcagtagctatgctatgcactgggtccgccagactccaggcaagggactagagtgggtggcagttatatcatatgatggaagtgactacgactacgcaggctccgtgaagggccgattcaccatctccagagacagttccaagaacatgctgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtatcactgtgtgagagagggtcgtggtaccgccgcctacggtctggacgtctggggccgagggaccacggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 93 88 [289] g133>a,A45>T(- - -)|g147>a|a190>g,N64>D(+ + -)|t192>c,N64>D(+ + -)|a193>t,K65>Y(- - -)|a195>c,K65>Y(- - -)|t196>g,Y66>D(- - -)|a206>g,D69>G(- - -)|a245>g,N82>S(- - -)|c257>t,T86>M(- - -)|g286>c,A96>P(- - -)|t307>c,Y103>H(+ - -)|c314>t,A105>V(+ - +)| g133>a,A45>T(- - -)|g147>a| a190>g,N64>D(+ + -)|t192>c,N64>D(+ + -)|a193>t,K65>Y(- - -)|a195>c,K65>Y(- - -)| t196>g,Y66>D(- - -)|a206>g,D69>G(- - -)|a245>g,N82>S(- - -)|c257>t,T86>M(- - -)|g286>c,A96>P(- - -)|t307>c,Y103>H(+ - -)| c314>t,A105>V(+ - +)| 320 223 210 13 1 12 3 2 2 2 1 0 1 0 1 0 0 1 78 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 0 1 1 0 1 0 0 0 0 0 117 114 108 6 0 6 2 0 1 1 0 0 0 0 1 0 0 1 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ct ggattcaccttcagtagctatgct atgcactgggtccgccagactccaggcaagggactagagtgggtggcagtt atatcatatgatggaagtgactac gactacgcaggctccgtgaagggccgattcaccatctccagagacagttccaagaacatgctgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtatcactgt gtgagagagggtcgtggtaccgccgcctacggtctggacgtc VREGRGTAAYGLDV IGHV3-30 IGHD2-2 IGHJ6 ggattcaccttcagtagctatgct atgcactgggtccgccagactccaggcaagggactagagtgggtggcagtt atatcatatgatggaagtgactac gactacgcaggctccgtgaagggccgattcaccatctccagagacagttccaagaacatgctgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtatcactgt gtgagagagggtcgtggtaccgccgcctacggtctggacgtc IGG2 12 213 8 4 3 4 5 8 1 0 7 4 JY8QFUQ01B7TWW 12802 productive Homsap IGHV3-48*01 F 995 95.41 208/218 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 3 8 8 13 8.8.13 [1.17.38.11] CARERGNSYGEIDYW in-frame - cctctggtttcaccttcaggagctctggcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagcagtagtactactaccaaatactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagaacgtggaaacagctatggagagattgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [289] a81>t|t105>g,S35>R(- - -)|a110>c,Y37>S(+ - -)|a112>g,S38>G(+ + -)|t171>c|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t194>a,I65>K(- + -)|a200>g,Y67>C(- - -)|a254>g,N85>S(- - -)| a81>t|t105>g,S35>R(- - -)|a110>c,Y37>S(+ - -)|a112>g,S38>G(+ + -)| t171>c|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t194>a,I65>K(- + -)| a200>g,Y67>C(- - -)|a254>g,N85>S(- - -)| 320 226 216 10 2 8 3 0 0 1 1 0 1 1 2 0 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 0 0 1 0 1 0 0 0 0 1 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 0 0 0 1 0 0 0 1 2 0 0 0 117 114 112 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggtttcaccttcaggagctctggc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagcagtagtactactaccaaa tactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagaacgtggaaacagctatggagagattgactac ARERGNSYGEIDY IGHV3-48 IGHD5-18 IGHJ4 ggtttcaccttcaggagctctggc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagcagtagtactactaccaaa tactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagaacgtggaaacagctatggagagattgactac IGG1 10 213 4 6 0 2 4 8 0 2 2 6 JY8QFUQ01B7VBT 11314 productive (see comment) Homsap IGHV4-61*02 F 890 90.7 195/215 nt 87.44 188/215 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-19*01 F 1 8 7 13 8.7.13 [2.16.38.11] CARQGDSSGPWNDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'CTGGTT' (6 nucleotides at position 23 to 28 in the user submitted sequence), (do not cause frameshift);in FR2-IMGT at codon 40 of V-REGION: 'G' (1 nucleotides at position 40 to 40 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 64 of V-REGION: 'A' (1 nucleotides at position 105 to 105 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 68 of V-REGION: 'A' (1 nucleotides at position 118 to 118 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 72 of V-REGION: 'C' (1 nucleotides at position 130 to 130 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 102 of V-REGION: 'C' (1 nucleotides at position 219 to 219 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 50 of V-REGION: 1 nucleotides (from position 66 in the user submitted sequence), (cause frameshift) gtctccggtggctccatcagtaCTGGTTattactactggGagctggatccggcagtccgccgggaagggactgaatggattgggcgcatgtctgccagagggggAcagcaactacaaAcccctccctccCagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtaCtttttgtgcgagacaaggggatagtagtggaccctggaatgactactggggccagggaatcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [297] t78>c|g103>a,G35>S(+ + -)|g107>a,S36>N(- - -)|c133>t,P45>S(+ - -)|g153>a|t165>c|c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>G(+ + -)|c194>g,T65>S(+ - +)|a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| t78>c| g103>a,G35>S(+ + -)|g107>a,S36>N(- - -)| c133>t,P45>S(+ - -)|g153>a|t165>c| c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>G(+ + -)|c194>g,T65>S(+ - +)| a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g319>c| 318 (320) 221 (223) 201 (202) 20 (21) 4 (5) 16 3 4 2 2 3 0 1 1 1 (2) 3 0 0 78 6 5 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 2 0 0 0 0 0 0 0 0 0 0 51 50 47 3 2 1 0 1 1 1 0 0 0 0 0 0 0 0 30 21 15 6 0 6 2 0 0 0 1 0 0 1 0 2 0 0 117 114 106 8 1 7 1 1 1 0 2 0 1 0 1 1 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctcc ggtggctccatcagtaattactac tggagctggatccggcagtccgccgggaagggactgaatggattgggcgc atgtctgccagagggggcagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac ARQGDSSGPWNDY IGHV4-61 IGHD6-19 IGHJ4 ggtggctccatcagtaattactac tggagctggatccggcagtccgccgggaagggactgaatggattgggcgc atgtctgccagagggggcagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac IGG2 19 209 10 9 6 10 4 9 3 0 8 8 JY8QFUQ01B7XXQ 14591 productive (see comment) Homsap IGHV3-49*03 F, or Homsap IGHV3-49*05 F 922 90.22 203/225 nt 89.78 202/225 nt Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD5-24*01 ORF 3 8 10 17 8.10.17 [2.17.38.11] CSRGLGDGYNLGYHALDIW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 47 of V-REGION: 'G' (1 nucleotides at position 56 to 56 in the user submitted sequence), (cause frameshift); gcttctggattcagttttggtgattattctatgatctggttccgccaggcaccggGggaaggggctggagtgcgtaggtttcattagaagcagagcatatggtgggacaacacaatacgccgcgtctgtgaaagacagattcaccatctcaagagatgatcccaaaggcatcgcctatttgcaaatgagcagtctgaaagtcgacgacacaggcgtgtatttttgtagtcggggattaggcgatggctacaatctgggttatcatgcccttgatatctggggccaagggacaatggtcaccgtctcttcagcatcccccgaccagccccaaggtcttcccgctgagccctcgacagcaccccccaagatgggaacgtggtcgtcgccatgcctggctccagggcttcttccc NA IGA2 71 84 [310, 309] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g112>t,A38>S(- + -)|g119>t,S40>I(- - -)|t135>a|a138>g|g156>c,W52>C(+ - -)|a176>g,K59>R(+ + +)|t180>a|g196>c,E66>Q(+ + -)|g221>a,G74>D(- - -)|t247>c,S83>P(+ - -)|a253>g,S85>G(+ + -)|c265>t|a275>g,N92>S(- - -)|c279>t|a286>g,T96>V(- - -)|c287>t,T96>V(- - -)|g291>c,E97>D(+ - +)|c299>g,A100>G(- + -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g112>t,A38>S(- + -)| g119>t,S40>I(- - -)|t135>a|a138>g|g156>c,W52>C(+ - -)| a176>g,K59>R(+ + +)|t180>a| g196>c,E66>Q(+ + -)|g221>a,G74>D(- - -)|t247>c,S83>P(+ - -)|a253>g,S85>G(+ + -)|c265>t|a275>g,N92>S(- - -)|c279>t|a286>g,T96>V(- - -)|c287>t,T96>V(- - -)|g291>c,E97>D(+ - +)|c299>g,A100>G(- + -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| c314>g,T105>S(+ - +)|a316>c|a318>g|a320>g| 313 (320) 226 (233) 204 (207) 22 (26) 5 (8) 17 (18) 5 (7) 1 5 1 0 (1) 0 1 2 3 2 (3) 2 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 1 0 0 0 0 0 0 1 1 0 51 51 47 4 2 2 1 0 0 0 0 0 0 1 1 0 1 0 30 30 28 2 1 1 1 0 0 0 0 0 0 1 0 0 0 0 117 114 101 13 2 11 3 1 4 1 0 0 1 0 2 1 0 0 1 (8) 1 1 (4) 0 (4) 0 (3) 0 (1) 0 (2) 0 0 0 0 (1) 0 0 0 0 0 (1) 0 0 ta,38-39(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,158-159(FR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,181-182(CDR2)|aa,192-193(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|ta,69-70(FR1)|tt,75-76(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,124-125(FR2)|ta,158-159(FR2)|tt,162-163(FR2)|tt,167-168(CDR2)|tt,180-181(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agct,72-75(FR1)|agct,118-121(FR2)|ggtt,122-125(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggta,156-159(FR2)|ggtt,160-163(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|ggca,220-223(FR3)|agca,253-256(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,72-75(FR1)|tgct,111-114(CDR1)|agct,118-121(FR2)|agct,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| gcttct ggattcagttttggtgattattct atgatctggttccgccaggcaccggggaaggggctggagtgcgtaggtttc attagaagcagagcatatggtgggacaaca caatacgccgcgtctgtgaaagacagattcaccatctcaagagatgatcccaaaggcatcgcctatttgcaaatgagcagtctgaaagtcgacgacacaggcgtgtatttttgt agtcggggattaggcgatggctacaatctgggttatcatgcccttgatatc SRGLGDGYNLGYHALDI IGHV3-49 IGHD5-24 IGHJ3 ggattcagttttggtgattattct atgatctggttccgccaggcaccggggaaggggctggagtgcgtaggtttc attagaagcagagcatatggtgggacaaca caatacgccgcgtctgtgaaagacagattcaccatctcaagagatgatcccaaaggcatcgcctatttgcaaatgagcagtctgaaagtcgacgacacaggcgtgtatttttgt agtcggggattaggcgatggctacaatctgggttatcatgcccttgatatc IGA2 22 219 12 10 6 13 6 9 4 1 13 4 JY8QFUQ01B81W4 16524 productive Homsap IGHV4-4*02 F, or Homsap IGHV4-4*03 F or Homsap IGHV4-4*04 F or Homsap IGHV4-4*05 F 950 93.12 203/218 nt NA Homsap IGHJ1*01 F 224 92.31 48/52 nt Homsap IGHD4-23*01 ORF 2 9 7 10 9.7.10 [1.17.38.11] CASHGGKYFPQW in-frame + tctctggtggctccatcagcagtagtaactggtggagttgggtccgccagcccccagggaaggggctggagtggattggacaaatccatcatggtgggggcaccaattacaacccgtccctcgagagtcgagtcactatatcagtagacaagtccaagaaccacctctccctgaccctgaactctgtgaccgccgcggacacggccgtttatcactgtgcgagccacggtggtaaatacttcccgcagtggggccagggcaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccag NA IGA2 71 95 [279, 281] g162>a|g163>c,E55>Q(+ + -)|t169>c,Y57>H(+ - -)|a175>g,S59>G(+ + -)|a190>g,S64>G(+ + -)|c198>t|a214>g,K72>E(+ - -)|c231>t|g258>c,Q86>H(- + -)|t259>c,F87>L(+ - -)|a269>c,K90>T(- - -)|g270>c,K90>T(- - -)|g275>a,S92>N(- - -)|g303>t|t307>c,Y103>H(+ - -)| g162>a|g163>c,E55>Q(+ + -)| t169>c,Y57>H(+ - -)|a175>g,S59>G(+ + -)|a190>g,S64>G(+ + -)| c198>t|a214>g,K72>E(+ - -)|c231>t|g258>c,Q86>H(- + -)|t259>c,F87>L(+ - -)|a269>c,K90>T(- - -)|g270>c,K90>T(- - -)|g275>a,S92>N(- - -)|g303>t|t307>c,Y103>H(+ - -)| a318>c,R106>S(- - -)|g319>c| 317 (320) 223 (226) 208 (209) 15 (17) 4 (5) 11 (12) 3 2 2 3 1 (2) 0 0 0 3 (4) 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 27 27 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 30 21 18 3 0 3 2 0 0 1 0 0 0 0 0 0 0 0 117 114 104 10 3 7 1 1 2 2 1 0 0 0 2 0 1 0 5 (8) 5 5 (6) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcagtagtaactgg tggagttgggtccgccagcccccagggaaggggctggagtggattggacaa atccatcatggtgggggcacc aattacaacccgtccctcgagagtcgagtcactatatcagtagacaagtccaagaaccacctctccctgaccctgaactctgtgaccgccgcggacacggccgtttatcactgt gcgagccacggtggtaaatacttcccgcag ASHGGKYFPQ IGHV4-4 IGHD4-23 IGHJ1 ggtggctccatcagcagtagtaactgg tggagttgggtccgccagcccccagggaaggggctggagtggattggacaa atccatcatggtgggggcacc aattacaacccgtccctcgagagtcgagtcactatatcagtagacaagtccaagaaccacctctccctgaccctgaactctgtgaccgccgcggacacggccgtttatcactgt gcgagccacggtggtaaatacttcccgcag IGA2 15 213 10 5 4 8 6 7 4 0 8 3 JY8QFUQ01B84I1 15604 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 902 90.05 199/221 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 2 10 7 12 10.7.12 [1.17.38.11] CARHSRLVRYFEFW in-frame + tctctggtggctccatcaacagtggtagttatcactgggcctggatccgccagtccccagggaaggggctggagtggattgggagcgtctcctatggtgggaacacctactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgtgcgagacattcgagactggtacggtactttgaattctggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaaggatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 89 [288, 289] g92>a,S31>N(- - -)|a103>g,S35>G(+ + -)|c111>t|t112>c,Y38>H(+ - -)|g119>c,G40>A(- + -)|c133>t,P45>S(+ - -)|t165>c|a166>g,I56>V(+ - +)|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a175>g,S59>G(+ + -)|g191>a,S64>N(- - -)|a215>t,K72>M(- + -)|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|c236>t,S79>F(- - -)|a240>c|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c261>g,F87>L(+ - -)|c271>g,L91>V(+ - +)|a291>g| g92>a,S31>N(- - -)|a103>g,S35>G(+ + -)|c111>t|t112>c,Y38>H(+ - -)| g119>c,G40>A(- + -)|c133>t,P45>S(+ - -)|t165>c| a166>g,I56>V(+ - +)|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a175>g,S59>G(+ + -)|g191>a,S64>N(- - -)| a215>t,K72>M(- + -)|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|c236>t,S79>F(- - -)|a240>c|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c261>g,F87>L(+ - -)|c271>g,L91>V(+ - +)|a291>g| 320 229 207 22 4 18 6 2 4 3 2 1 1 0 1 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 1 3 1 1 1 1 0 0 0 0 0 0 0 0 51 51 48 3 1 2 0 0 1 1 0 0 0 0 1 0 0 0 30 21 16 5 0 5 2 1 0 1 1 0 0 0 0 0 0 0 117 114 104 10 2 8 3 0 2 0 1 1 1 0 0 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcaacagtggtagttatcac tgggcctggatccgccagtccccagggaaggggctggagtggattgggagc gtctcctatggtgggaacacc tactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgt gcgagacattcgagactggtacggtactttgaattc ARHSRLVRYFEF IGHV4-39 IGHD5-18 IGHJ4 ggtggctccatcaacagtggtagttatcac tgggcctggatccgccagtccccagggaaggggctggagtggattgggagc gtctcctatggtgggaacacc tactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgt gcgagacattcgagactggtacggtactttgaattc IGA2 22 216 15 7 6 10 9 12 3 1 10 8 JY8QFUQ01B8ABJ 18606 productive Homsap IGHV1-2*02 F 903 91.59 196/214 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD6-13*01 F 2 8 8 17 8.8.17 [0.17.38.11] CARGGTVTAGGPRIVFGYW in-frame + tggctacaccttcaccgactactatatacactgggtgcgacaggcccctggacaagggcttgagtggatgggatggatcaaccctgacggtggtagcacaaagtatgcacagaaatttcagggcagggtcgccgtgaccagggacacgtcaattagcacagcctacattgaggtgaccagactgacatctgacgacacggccgtgtattattgtgccagaggagggactgtgacagcaggtggtcctcgtattgtctttgggtactggggccagggaacgttggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA2 71 100 [296] a81>c|g107>a,G36>D(- - -)|g117>a,M39>I(+ + -)|a175>g,N59>D(+ + -)|a184>g,S62>G(+ + -)|g190>a,G64>S(+ + -)|c198>g,N66>K(+ - -)|g210>a|a229>g,T77>A(- - -)|a232>g,M78>V(+ - -)|c249>a|c252>t|g267>t,M89>I(+ + -)|c271>g,L91>V(+ - +)|g275>c,S92>T(+ - +)|g279>a|g284>c,R95>T(- - -)|c309>t|g315>c| a81>c|g107>a,G36>D(- - -)| g117>a,M39>I(+ + -)| a175>g,N59>D(+ + -)|a184>g,S62>G(+ + -)|g190>a,G64>S(+ + -)| c198>g,N66>K(+ - -)|g210>a|a229>g,T77>A(- - -)|a232>g,M78>V(+ - -)|c249>a|c252>t|g267>t,M89>I(+ + -)|c271>g,L91>V(+ - +)|g275>c,S92>T(+ - +)|g279>a|g284>c,R95>T(- - -)|c309>t| g315>c|a320>g| 319 (320) 221 (222) 202 19 (20) 7 (8) 12 4 (5) 5 2 0 1 1 0 0 3 2 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 1 0 0 1 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 2 1 0 0 0 0 0 0 0 0 0 0 117 114 102 12 5 7 2 2 2 0 0 1 0 0 2 2 1 0 7 (8) 7 6 1 (2) 1 (2) 0 0 (1) 0 0 0 0 0 0 0 1 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggctacaccttcaccgactactat atacactgggtgcgacaggcccctggacaagggcttgagtggatgggatgg atcaaccctgacggtggtagcaca aagtatgcacagaaatttcagggcagggtcgccgtgaccagggacacgtcaattagcacagcctacattgaggtgaccagactgacatctgacgacacggccgtgtattattgt gccagaggagggactgtgacagcaggtggtcctcgtattgtctttgggtac ARGGTVTAGGPRIVFGY IGHV1-2 IGHD6-13 IGHJ4 ggctacaccttcaccgactactat atacactgggtgcgacaggcccctggacaagggcttgagtggatgggatgg atcaaccctgacggtggtagcaca aagtatgcacagaaatttcagggcagggtcgccgtgaccagggacacgtcaattagcacagcctacattgaggtgaccagactgacatctgacgacacggccgtgtattattgt gccagaggagggactgtgacagcaggtggtcctcgtattgtctttgggtac IGA2 18 213 11 7 7 13 4 5 5 1 8 4 JY8QFUQ01B8AC6 12519 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 892 89.95 197/219 nt NA Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD5-24*01 ORF 3 8 8 16 8.8.16 [2.17.38.11] CVRYDYSRLHRSNYFGPW in-frame - gtctctggattcaccttcatcagttatggcatgagttgggtccgccagtttccagggaaggggctggagtgggtctcatctattagtgattatggtaataccgcattctacgcagactccgtgaagggccggttcaccatctccagagacaattccaacaacacgctgtttctgcaaatgagcagcctgagagccgaggacacggccgtttattattgtgtgagatatgactactcacgcctacacagatccaactacttcggcccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcc NA IGA1 100 100 [298] c74>t,A25>V(+ - +)|t90>c|g104>t,S35>I(- - -)|c108>t|c113>g,A38>G(- + -)|c120>t|g133>t,A45>F(+ - -)|c134>t,A45>F(+ - -)|g163>t,A55>S(- + -)|g173>a,G58>D(- - -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|g187>a,G63>N(- - -)|g188>a,G63>N(- - -)|g191>c,S64>T(+ - +)|a193>g,T65>A(- - -)|a197>t,Y66>F(- + -)|g252>c,K84>N(+ - -)|a263>t,Y88>F(- + -)|a275>g,N92>S(- - -)|a303>t|c309>t|c314>t,A105>V(+ - +)| c74>t,A25>V(+ - +)| t90>c|g104>t,S35>I(- - -)|c108>t|c113>g,A38>G(- + -)| c120>t|g133>t,A45>F(+ - -)|c134>t,A45>F(+ - -)|g163>t,A55>S(- + -)| g173>a,G58>D(- - -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|g187>a,G63>N(- - -)|g188>a,G63>N(- - -)|g191>c,S64>T(+ - +)|a193>g,T65>A(- - -)| a197>t,Y66>F(- + -)|g252>c,K84>N(+ - -)|a263>t,Y88>F(- + -)|a275>g,N92>S(- - -)|a303>t|c309>t| c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)|g319>t| 316 (320) 223 (227) 200 (202) 23 (25) 5 (6) 18 (19) 2 (3) 4 6 1 0 0 4 0 2 1 3 (4) 0 78 6 5 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 0 1 1 0 0 0 0 0 1 1 0 51 51 47 4 1 3 0 0 2 0 0 0 0 0 0 0 2 0 30 24 17 7 0 7 1 4 0 0 0 0 1 0 1 0 0 0 117 114 108 6 2 4 1 0 1 0 0 0 3 0 1 0 0 0 4 (8) 4 3 (5) 1 (3) 0 (1) 1 (2) 0 (1) 0 1 0 0 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtctct ggattcaccttcatcagttatggc atgagttgggtccgccagtttccagggaaggggctggagtgggtctcatct attagtgattatggtaataccgca ttctacgcagactccgtgaagggccggttcaccatctccagagacaattccaacaacacgctgtttctgcaaatgagcagcctgagagccgaggacacggccgtttattattgt gtgagatatgactactcacgcctacacagatccaactacttcggcccc VRYDYSRLHRSNYFGP IGHV3-23 IGHD5-24 IGHJ5 ggattcaccttcatcagttatggc atgagttgggtccgccagtttccagggaaggggctggagtgggtctcatct attagtgattatggtaataccgca ttctacgcagactccgtgaagggccggttcaccatctccagagacaattccaacaacacgctgtttctgcaaatgagcagcctgagagccgaggacacggccgtttattattgt gtgagatatgactactcacgcctacacagatccaactacttcggcccc IGA1 21 213 11 10 8 14 3 7 3 2 7 9 JY8QFUQ01B8DLI 16027 productive Homsap IGHV3-7*01 F 881 90.23 194/215 nt NA Homsap IGHJ3*01 F 223 94 47/50 nt Homsap IGHD1-14*01 ORF 1 8 8 14 8.8.14 [0.17.38.11] CARVIPGRGNYAFDVW in-frame - ctggattcgactttggtagttattggatgagttgggtccgccaggctccagggaagggactggagtgggtggccagcataaagcgagatgcaagtgagaagtaccatgtggaatctgtgcagagacgattcaccatcttcagagacaacgtcaggaactcactgtatttgcagatgaacagcctgagagacgaggacacggctgtgtattactgtgcgagagttattcccggccggggaaactatgcttttgatgtctggggccgagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggttcagggcttcttccccca NA IGA2 79 95 [288, 287] a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|a103>g,S35>G(+ + -)|c108>t|c120>t|g147>a|a164>g,N55>S(- - -)|a173>g,Q58>R(+ - -)|g185>c,G62>A(- + -)|a195>g|t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a214>c,K72>Q(+ - -)|g220>a,G74>R(- - -)|c222>a,G74>R(- - -)|c236>t,S79>F(- - -)|c248>t,A83>V(+ - +)|a251>g,K84>R(+ + +)|c265>t|a270>g|c287>a,A96>D(- - -)| a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|a103>g,S35>G(+ + -)|c108>t| c120>t|g147>a|a164>g,N55>S(- - -)| a173>g,Q58>R(+ - -)|g185>c,G62>A(- + -)|a195>g| t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a214>c,K72>Q(+ - -)|g220>a,G74>R(- - -)|c222>a,G74>R(- - -)|c236>t,S79>F(- - -)|c248>t,A83>V(+ - +)|a251>g,K84>R(+ + +)|c265>t|a270>g|c287>a,A96>D(- - -)| a320>t| 319 (320) 222 (223) 201 21 (22) 6 (7) 15 7 2 5 1 1 4 0 (1) 0 1 0 0 0 78 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 2 0 1 0 0 1 0 0 0 0 0 0 51 51 48 3 2 1 1 1 1 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 2 0 0 0 0 0 0 0 1 0 0 0 117 114 103 11 2 9 2 1 3 1 1 3 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ct ggattcgactttggtagttattgg atgagttgggtccgccaggctccagggaagggactggagtgggtggccagc ataaagcgagatgcaagtgagaag taccatgtggaatctgtgcagagacgattcaccatcttcagagacaacgtcaggaactcactgtatttgcagatgaacagcctgagagacgaggacacggctgtgtattactgt gcgagagttattcccggccggggaaactatgcttttgatgtc ARVIPGRGNYAFDV IGHV3-7 IGHD1-14 IGHJ3 ggattcgactttggtagttattgg atgagttgggtccgccaggctccagggaagggactggagtgggtggccagc ataaagcgagatgcaagtgagaag taccatgtggaatctgtgcagagacgattcaccatcttcagagacaacgtcaggaactcactgtatttgcagatgaacagcctgagagacgaggacacggctgtgtattactgt gcgagagttattcccggccggggaaactatgcttttgatgtc IGA2 21 213 15 6 7 12 8 9 4 2 10 5 JY8QFUQ01B8DVS 16241 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 923 91.28 199/218 nt 90.37 197/218 nt Homsap IGHJ1*01 F (see comment) 133 74 37/50 nt Homsap IGHD5-18*01 F 3 8 8 6 8.8.6 [1.17.38.11] CASYGQWL in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 48 of V-REGION: 'G' (1 nucleotides at position 59 to 59 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 64 of V-REGION: 'ATTT' (4 nucleotides at position 101 to 104 in the user submitted sequence), (cause frameshift); cctcgggactcatgtttagcagctatgccatgacctgggtccgccaggctccagggaaGggggctggagtgggtctcaagtgtcagtagtagtactggttATTTtcacatactacacagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtatattattgcgctagttacgggcagtggctgggccagggaacccaggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG4 100 88 [273] t78>g|t82>c,F28>L(+ - -)|c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|g119>c,S40>T(+ - +)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g172>a,G58>S(+ + -)|g184>a,G62>T(+ - -)|g185>c,G62>T(+ - -)|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)|g202>a,A68>T(- - -)|a275>g,N92>S(- - -)|g291>a|c309>t|t312>c| t78>g| t82>c,F28>L(+ - -)|c86>t,T29>M(- - -)|c87>g,T29>M(- - -)| g119>c,S40>T(+ - +)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g172>a,G58>S(+ + -)|g184>a,G62>T(+ - -)|g185>c,G62>T(+ - -)|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)| g202>a,A68>T(- - -)|a275>g,N92>S(- - -)|g291>a|c309>t|t312>c| g315>t|a317>g,K106>S(- - -)|a318>t,K106>S(- - -)|g319>t| 314 (320) 220 (226) 201 (203) 19 (23) 4 (6) 15 (17) 2 (3) 5 2 3 0 0 1 (2) 0 2 2 1 (3) 1 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 36 24 21 3 0 3 0 0 1 1 0 0 0 0 0 1 0 0 51 51 48 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 30 24 17 7 0 7 1 2 0 1 0 0 1 0 1 0 1 0 117 114 109 5 3 2 1 2 1 1 0 0 0 0 0 0 0 0 2 (8) 2 2 (4) 0 (4) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (2) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctcg ggactcatgtttagcagctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcaagt gtcagtagtagtactggtttcaca tactacacagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtatattattgc gctagttacgggcagtgg ASYGQW IGHV3-23 IGHD5-18 IGHJ1 ggactcatgtttagcagctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcaagt gtcagtagtagtactggtttcaca tactacacagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtatattattgc gctagttacgggcagtgg IGG4 18 213 12 6 7 12 5 6 3 0 5 10 JY8QFUQ01B8JYP 7765 productive (see comment) Homsap IGHV3-11*01 F 867 89.72 192/214 nt 88.79 190/214 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-2*01 F 2 8 7 14 8.7.14 [1.16.38.11] CARDPYCSSTNCYPYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 59 in the user submitted sequence), (cause frameshift)in CDR2-IMGT, from codon 62 of V-REGION: 3 nucleotides (from position 94 in the user submitted sequence), (do not cause frameshift) cctctggattcaccctcagtgactactacatgagttggttccgccaggctccagggaagggctggagtggctttcatacattgcaggaagtggaaccacatattacgcagattctgtgaagggccgattcaccatctccagggacaatgccgagcactcggtatacctgcaaatgaacagcctgagagtcgaagacacggccgtgtattactgtgcgagagatccctattgtagtagtaccaactgctatccctactggggccagggaacccaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] t88>c,F30>L(+ - -)|c120>t|a124>t,I42>F(+ - -)|g157>c,V53>L(+ - +)|a169>g,S57>A(- + -)|g170>c,S57>A(- + -)|t171>a,S57>A(- + -)|a172>g,S58>G(+ + -)|t174>a,S58>G(+ + -)|t189>a|t194>c,I65>T(- - -)|c198>t|c207>t|c246>t|a250>g,K84>E(+ - -)|a253>c,N85>H(- - -)|a258>g|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t264>c|c287>t,A96>V(+ - +)|g291>a| t88>c,F30>L(+ - -)| c120>t|a124>t,I42>F(+ - -)|g157>c,V53>L(+ - +)| a169>g,S57>A(- + -)|g170>c,S57>A(- + -)|t171>a,S57>A(- + -)|a172>g,S58>G(+ + -)|t174>a,S58>G(+ + -)|t189>a|t194>c,I65>T(- - -)| c198>t|c207>t|c246>t|a250>g,K84>E(+ - -)|a253>c,N85>H(- - -)|a258>g|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t264>c|c287>t,A96>V(+ - +)|g291>a| 320 222 200 22 8 14 4 2 5 3 1 0 1 3 2 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 51 50 47 3 1 2 0 0 1 0 0 0 1 0 1 0 0 0 30 21 14 7 1 6 2 0 0 1 0 0 0 3 1 0 0 0 117 114 103 11 6 5 2 2 4 1 1 0 0 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccctcagtgactactac atgagttggttccgccaggctccagggaagggctggagtggctttcatac attgcaggaagtggaaccaca tattacgcagattctgtgaagggccgattcaccatctccagggacaatgccgagcactcggtatacctgcaaatgaacagcctgagagtcgaagacacggccgtgtattactgt gcgagagatccctattgtagtagtaccaactgctatccctac ARDPYCSSTNCYPY IGHV3-11 IGHD2-2 IGHJ4 ggattcaccctcagtgactactac atgagttggttccgccaggctccagggaagggctggagtggctttcatac attgcaggaagtggaaccaca tattacgcagattctgtgaagggccgattcaccatctccagggacaatgccgagcactcggtatacctgcaaatgaacagcctgagagtcgaagacacggccgtgtattactgt gcgagagatccctattgtagtagtaccaactgctatccctac IGA1 22 209 14 8 7 10 7 12 7 1 7 7 JY8QFUQ01B8T6Y 16885 productive Homsap IGHV3-9*01 F 891 90.32 196/217 nt NA Homsap IGHJ6*02 F 238 87.1 54/62 nt Homsap IGHD4-17*01 F 2 8 8 12 8.8.12 [1.17.38.11] CARTYGDHDGMDVW in-frame + ctctggattcacctttgatgactatggcatgcactgggtccggcaaggtccagggaagggcctggagtgggtctcaagtattagttggaacagtggtaagatagactatgcggacgctgtgaagggccgattcatcacatccagagacaatgccaagaactccatctatttgcaaatgaacagtctgagagatgacgacacggccttctacttctgtgcaaggacttacggcgaccatgacggaatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] t108>c|c113>g,A38>G(- + -)|c134>g,A45>G(- + -)|g163>a,G55>S(+ + -)|t177>c|g191>a,S64>K(- - -)|c192>g,S64>K(- - -)|g197>a,G66>D(- - -)|t208>g,S70>A(- + -)|c230>t,T77>I(- - -)|t233>c,I78>T(- - -)|c234>a,I78>T(- - -)|c246>t|c259>a,L87>I(+ + +)|g261>c,L87>I(+ + +)|c265>t|c287>a,A96>D(- - -)|g291>c,E97>D(+ - +)|g303>c,L101>F(+ - -)|t306>c|a308>t,Y103>F(- + -)| t108>c|c113>g,A38>G(- + -)| c134>g,A45>G(- + -)|g163>a,G55>S(+ + -)| t177>c|g191>a,S64>K(- - -)|c192>g,S64>K(- - -)| g197>a,G66>D(- - -)|t208>g,S70>A(- + -)|c230>t,T77>I(- - -)|t233>c,I78>T(- - -)|c234>a,I78>T(- - -)|c246>t|c259>a,L87>I(+ + +)|g261>c,L87>I(+ + +)|c265>t|c287>a,A96>D(- - -)|g291>c,E97>D(+ - +)|g303>c,L101>F(+ - -)|t306>c|a308>t,Y103>F(- + -)| a317>g,K106>R(+ + +)|a318>g,K106>R(+ + +)|g319>a,D107>T(- + -)|a320>c,D107>T(- + -)|a322>t| 316 (322) 221 (227) 200 (201) 21 (26) 5 (6) 16 (20) 0 (2) 3 (4) 3 4 0 (1) 3 1 (2) 0 3 3 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 0 1 0 0 0 0 0 1 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 0 1 0 0 30 24 21 3 1 2 0 1 0 1 0 0 0 0 0 1 0 0 117 114 100 14 3 11 0 1 3 2 0 3 1 0 3 0 0 1 4 (10) 4 4 (5) 0 (5) 0 (1) 0 (4) 0 (2) 0 (1) 0 0 0 (1) 0 0 (1) 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttgatgactatggc atgcactgggtccggcaaggtccagggaagggcctggagtgggtctcaagt attagttggaacagtggtaagata gactatgcggacgctgtgaagggccgattcatcacatccagagacaatgccaagaactccatctatttgcaaatgaacagtctgagagatgacgacacggccttctacttctgt gcaaggacttacggcgaccatgacggaatggacgtc ARTYGDHDGMDV IGHV3-9 IGHD4-17 IGHJ6 ggattcacctttgatgactatggc atgcactgggtccggcaaggtccagggaagggcctggagtgggtctcaagt attagttggaacagtggtaagata gactatgcggacgctgtgaagggccgattcatcacatccagagacaatgccaagaactccatctatttgcaaatgaacagtctgagagatgacgacacggccttctacttctgt gcaaggacttacggcgaccatgacggaatggacgtc IGA1 21 213 10 11 6 15 4 6 3 2 13 3 JY8QFUQ01B8WF3 9189 productive Homsap IGHV3-72*01 F 975 93.27 208/223 nt NA Homsap IGHJ6*02 F (see comment) 211 82.26 51/62 nt Homsap IGHD4-11*01 ORF 1 8 10 13 8.10.13 [1.17.38.11] CVRGLQSNYYGMDVR in-frame + Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) ctctggattcaccttcagtgaccactacatggactgggtccgccaggctccagggaaggggctggagtgggttggccgtagtagagacaaaattaacagttacagtacagaatatgccgcgtctgcgaagggcagattcaccatctcaagagatgaatcaaagaatttactgtatctgcaaatgaacagcctgagaagcgaggacacggccgtatattactgtgttagaggcctacagagtaattactacggtatggacgtcaggggccaggggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 84 [293, 291] c167>g,T56>S(+ - +)|a172>g,N58>D(+ + -)|g178>a,A60>I(+ - +)|c179>t,A60>I(+ - +)|c191>g,T64>S(+ - +)|c192>t,T64>S(+ - +)|c201>t|t212>c,V71>A(+ - +)|a216>g|t246>a,D82>E(+ - +)|c255>t|c257>t,S86>L(- - -)|a284>g,K95>R(+ + +)|c287>g,T96>S(+ - +)|g303>a|c314>t,A105>V(+ - +)| c167>g,T56>S(+ - +)|a172>g,N58>D(+ + -)|g178>a,A60>I(+ - +)|c179>t,A60>I(+ - +)|c191>g,T64>S(+ - +)|c192>t,T64>S(+ - +)| c201>t|t212>c,V71>A(+ - +)|a216>g|t246>a,D82>E(+ - +)|c255>t|c257>t,S86>L(- - -)|a284>g,K95>R(+ + +)|c287>g,T96>S(+ - +)|g303>a| c314>t,A105>V(+ - +)|a320>g| 319 (320) 230 (231) 214 16 (17) 4 (5) 12 3 (4) 2 6 1 0 0 0 1 0 3 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 24 6 0 6 1 1 2 0 0 0 0 0 0 2 0 0 117 114 105 9 4 5 2 1 3 1 0 0 0 1 0 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,165-168(FR2-CDR2)|agct,177-180(CDR2)|aact,253-256(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tgct,312-315(FR3)| ctct ggattcaccttcagtgaccactac atggactgggtccgccaggctccagggaaggggctggagtgggttggccgt agtagagacaaaattaacagttacagtaca gaatatgccgcgtctgcgaagggcagattcaccatctcaagagatgaatcaaagaatttactgtatctgcaaatgaacagcctgagaagcgaggacacggccgtatattactgt gttagaggcctacagagtaattactacggtatggacgtc VRGLQSNYYGMDV IGHV3-72 IGHD4-11 IGHJ6 ggattcaccttcagtgaccactac atggactgggtccgccaggctccagggaaggggctggagtgggttggccgt agtagagacaaaattaacagttacagtaca gaatatgccgcgtctgcgaagggcagattcaccatctcaagagatgaatcaaagaatttactgtatctgcaaatgaacagcctgagaagcgaggacacggccgtatattactgt gttagaggcctacagagtaattactacggtatggacgtc IGA2 15 219 11 4 7 10 4 5 4 0 5 6 JY8QFUQ01B8YQQ 17855 productive Homsap IGHV3-30*01 F, or Homsap IGHV3-30*17 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F (see comment) 177 85.42 41/48 nt Homsap IGHD2-21*01 F 2 8 8 14 8.8.14 [1.17.38.11] CATDRKYSGVRGDDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcaccttcaggaactatgctatgcactgggtccgccaggctcctggcaaggggctagaatgggtggcttttatatcatatgatggaagtagtcaatactacgcagactccgtgaagggccgattcaccatctccagagacaactccaagaacacactttatctgcaaatgaacagcctgagaggtgacgacacggctgtgttttactgtgcgacggaccggaaatatagtggtgtacgaggcgatgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [290] t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|a138>t|g153>a|a162>t|g163>t,V55>F(+ - -)|a191>g,N64>S(- - -)|a193>c,K65>Q(+ - -)|t246>c|g258>a|g261>t|c287>g,A96>G(- + -)|g291>c,E97>D(+ - +)|a305>t,Y102>F(- + -)| t105>g,S35>R(- - -)|g107>a,S36>N(- - -)| a138>t|g153>a|a162>t|g163>t,V55>F(+ - -)| a191>g,N64>S(- - -)|a193>c,K65>Q(+ - -)| t246>c|g258>a|g261>t|c287>g,A96>G(- + -)|g291>c,E97>D(+ - +)|a305>t,Y102>F(- + -)| g317>c,R106>T(- - -)|a318>g,R106>T(- - -)| 316 (320) 221 (225) 207 (209) 14 (16) 6 8 (10) 1 (2) 3 0 1 1 0 3 0 1 (2) 1 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 51 51 47 4 3 1 0 1 0 0 0 0 2 0 0 0 1 0 30 24 22 2 0 2 1 0 0 0 1 0 0 0 0 0 0 0 117 114 108 6 3 3 0 1 0 1 0 0 1 0 1 1 1 0 4 (8) 4 4 (6) 0 (2) 0 0 (2) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcaggaactatgct atgcactgggtccgccaggctcctggcaaggggctagaatgggtggctttt atatcatatgatggaagtagtcaa tactacgcagactccgtgaagggccgattcaccatctccagagacaactccaagaacacactttatctgcaaatgaacagcctgagaggtgacgacacggctgtgttttactgt gcgacggaccggaaatatagtggtgtacgaggcgatgactcc ATDRKYSGVRGDDS IGHV3-30 IGHD2-21 IGHJ4 ggattcaccttcaggaactatgct atgcactgggtccgccaggctcctggcaaggggctagaatgggtggctttt atatcatatgatggaagtagtcaa tactacgcagactccgtgaagggccgattcaccatctccagagacaactccaagaacacactttatctgcaaatgaacagcctgagaggtgacgacacggctgtgttttactgt gcgacggaccggaaatatagtggtgtacgaggcgatgactcc IGA1 14 213 5 9 3 7 2 7 6 0 4 4 JY8QFUQ01B90UJ 16878 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 896 90.37 197/218 nt 89.45 195/218 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [2.16.38.11] CVRAGFGELSGFDCW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'C' (1 nucleotides at position 16 to 16 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 40 of V-REGION: 1 nucleotides (from position 35 in the user submitted sequence), (cause frameshift) cctcctggattcaccCtttggttattatggcatgactgggtccgccaactccgggggagggggctggagtgggtctctggtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [290] g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>P(+ - -)|a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|t135>c,A45>L(+ - +)|c137>g,P46>R(- - -)|a138>g,P46>R(- - -)|a143>g,K48>R(+ + +)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>P(+ - -)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|t135>c,A45>L(+ - +)|c137>g,P46>R(- - -)|a138>g,P46>R(- - -)|a143>g,K48>R(+ + +)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 225 (226) 203 22 (23) 2 (3) 20 3 3 3 3 1 (2) 0 2 0 4 2 1 0 78 6 3 3 0 3 0 0 1 1 0 0 0 0 1 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 50 44 6 0 6 2 0 1 1 0 0 0 0 1 1 0 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| cctcct ggattcacctttggttattatggc atgactgggtccgccaactccgggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgactgggtccgccaactccgggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 18 212 9 9 4 10 5 8 2 0 12 4 JY8QFUQ01B93WH 18312 productive (see comment) Homsap IGHV4-61*02 F 987 94.55 208/220 nt 93.64 206/220 nt Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD3-10*01 F 3 10 7 11 10.7.11 [1.17.37.11] CATVARRGAFDIW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 54 of V-REGION: 'G' (1 nucleotides at position 82 to 82 in the user submitted sequence), (cause frameshift); in FR3-IMGT, from codon 96 of V-REGION: 1 nucleotides (from position 195 in the user submitted sequence), (cause frameshift) tctctggtgggtccatcagcagtactagttactactggagctggatccggcagcccgccgggaagggactggagtggatggGggcgtatctataccagtgggatcaccaactacaacccctccctcgagagtcgagtcaccttttcagtggacacgtccaagaaccagttctccctgaagctgaagtctgtgaccccgcagacacggccgtttattactgtgcgacagtggcgcgccggggggcttttgatatctggggccaagggacactggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [286] c84>g|g103>a,G35>T(+ - -)|g104>c,G35>T(+ - -)|t159>g,I53>M(+ + -)|g191>t,S64>I(- - -)|a214>g,K72>E(+ - -)|a232>t,I78>F(+ - -)|a234>t,I78>F(+ - -)|a240>g|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g303>t|g317>c,R106>T(- - -)| c84>g|g103>a,G35>T(+ - -)|g104>c,G35>T(+ - -)| t159>g,I53>M(+ + -)| g191>t,S64>I(- - -)| a214>g,K72>E(+ - -)|a232>t,I78>F(+ - -)|a234>t,I78>F(+ - -)|a240>g|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g303>t| g317>c,R106>T(- - -)|a320>t| 319 (320) 227 (228) 214 13 (14) 3 (4) 10 2 2 0 0 0 0 2 (3) 0 2 2 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 1 2 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 30 21 20 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 117 113 106 7 2 5 2 1 0 0 0 0 2 0 0 1 1 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 0 0 0 0 0 (1) 0 1 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgggtccatcagcagtactagttactac tggagctggatccggcagcccgccgggaagggactggagtggatggggcgt atctataccagtgggatcacc aactacaacccctccctcgagagtcgagtcaccttttcagtggacacgtccaagaaccagttctccctgaagctgaagtctgtgaccccgcagacacggccgtttattactgt gcgacagtggcgcgccggggggcttttgatatc ATVARRGAFDI IGHV4-61 IGHD3-10 IGHJ3 ggtgggtccatcagcagtactagttactac tggagctggatccggcagcccgccgggaagggactggagtggatggggcgt atctataccagtgggatcacc aactacaacccctccctcgagagtcgagtcaccttttcagtggacacgtccaagaaccagttctccctgaagctgaagtctgtgaccccgcagacacggccgtttattactgt gcgacagtggcgcgccggggggcttttgatatc IGG2 12 215 4 8 2 7 2 5 2 1 6 3 JY8QFUQ01B978H 18827 productive (see comment) Homsap IGHV3-74*03 F (see comment) 793 84.93 186/219 nt 84.02 184/219 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-13*01 F 1 8 8 13 8.8.13 [2.17.38.11] CVRDSDSSASYFAHW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (84,93% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR1-IMGT at codon 30 of V-REGION: 'TC' (2 nucleotides at position 18 to 19 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 72 of V-REGION: 'A' (1 nucleotides at position 127 to 127 in the user submitted sequence), (cause frameshift); gcctctggattcacgttTCcacacctcctggatggcactgggtccgccaaggtccaggggaggggctaatgtgggtctcacgaatcaatactgatgggagtaacacaatgtacgcggactccgtaaAagggccggttcaccatttccagagacaatgccaagaatacggtgtttctgcaaatgaacagtctgaaagccgacgacacggctgtctattattgtgttagagattcagacagtagcgcctcgtacttcgcccactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [292] c87>g|g104>c,S35>T(+ - +)|t105>a,S35>T(+ - +)|a106>c,S36>P(+ - -)|g107>c,S36>P(+ - -)|c108>t,S36>P(+ - -)|t109>c,Y37>P(+ - -)|a110>c,Y37>P(+ - -)|c111>t,Y37>P(+ - -)|t112>g,W38>G(- - -)|g114>a,W38>G(- - -)|a115>t,M39>W(+ - -)|t116>g,M39>W(+ - -)|c134>g,A45>G(- + -)|a142>g,K48>E(+ - -)|g150>a|g151>a,V51>M(+ - -)|t165>a|t168>c|g173>c,S58>T(+ - +)|g191>a,S64>N(- - -)|c197>t,T66>M(- - -)|g213>a|a225>g|c234>t|c246>t|c255>t|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|g284>a,R95>K(+ + +)|g291>c,E97>D(+ - +)|g303>c|c309>t|c314>t,A105>V(+ - +)|a315>t,A105>V(+ - +)| c87>g|g104>c,S35>T(+ - +)|t105>a,S35>T(+ - +)|a106>c,S36>P(+ - -)|g107>c,S36>P(+ - -)|c108>t,S36>P(+ - -)|t109>c,Y37>P(+ - -)|a110>c,Y37>P(+ - -)|c111>t,Y37>P(+ - -)|t112>g,W38>G(- - -)|g114>a,W38>G(- - -)| a115>t,M39>W(+ - -)|t116>g,M39>W(+ - -)|c134>g,A45>G(- + -)|a142>g,K48>E(+ - -)|g150>a|g151>a,V51>M(+ - -)|t165>a| t168>c|g173>c,S58>T(+ - +)|g191>a,S64>N(- - -)| c197>t,T66>M(- - -)|g213>a|a225>g|c234>t|c246>t|c255>t|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|g284>a,R95>K(+ + +)|g291>c,E97>D(+ - +)|g303>c|c309>t| c314>t,A105>V(+ - +)|a315>t,A105>V(+ - +)| 320 227 192 35 11 24 2 6 8 2 2 0 3 2 5 3 0 2 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 13 11 1 10 0 1 2 1 2 0 0 1 2 1 0 1 51 51 44 7 2 5 1 2 0 0 0 0 1 1 0 1 0 1 30 24 21 3 1 2 0 1 0 1 0 0 0 0 1 0 0 0 117 114 102 12 7 5 1 2 5 0 0 0 1 0 2 1 0 0 8 8 6 2 0 2 0 0 1 0 0 0 1 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacgttcacacctcctgga tggcactgggtccgccaaggtccaggggaggggctaatgtgggtctcacga atcaatactgatgggagtaacaca atgtacgcggactccgtaaagggccggttcaccatttccagagacaatgccaagaatacggtgtttctgcaaatgaacagtctgaaagccgacgacacggctgtctattattgt gttagagattcagacagtagcgcctcgtacttcgcccac VRDSDSSASYFAH IGHV3-74 IGHD6-13 IGHJ4 ggattcacgttcacacctcctgga tggcactgggtccgccaaggtccaggggaggggctaatgtgggtctcacga atcaatactgatgggagtaacaca atgtacgcggactccgtaaagggccggttcaccatttccagagacaatgccaagaatacggtgtttctgcaaatgaacagtctgaaagccgacgacacggctgtctattattgt gttagagattcagacagtagcgcctcgtacttcgcccac IGA1 33 213 17 16 13 21 4 12 9 2 10 12 JY8QFUQ01B9C4B 15166 productive Homsap IGHV3-66*02 F 898 91.55 195/213 nt NA Homsap IGHJ4*02 F (see comment) 186 87.5 42/48 nt Homsap IGHD1-26*01 F 1 8 7 13 8.7.13 [1.17.38.11] CASVGVGATVAFQYW in-frame + Other possibilities: Homsap_IGHJ1*01 (highest number of consecutive identical nucleotides) tctggattcaccgtcagtgatagttacatgagctgggtccgccaggctccagggaaggggctggagtgggtctcgattatttataggggaggtaccacatattatgccgactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctatatcttcaaatgaacaccctgagaggtgaggacacggctctatattactgtgcgagcgtcggagtgggagctacagtcgcctttcaatactggggccagggcaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccgcaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 71 95 [283, 284] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|a110>g,N37>S(- - -)|c111>t,N37>S(- - -)|a162>g|g163>a,V55>I(+ - +)|c174>g,S58>R(- - -)|t177>a|g191>c,S64>T(+ - +)|c198>t|c201>t|a204>c|g261>a|g278>c,S93>T(+ - +)|c287>g,A96>G(- + -)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|a110>g,N37>S(- - -)|c111>t,N37>S(- - -)| a162>g|g163>a,V55>I(+ - +)| c174>g,S58>R(- - -)|t177>a|g191>c,S64>T(+ - +)| c198>t|c201>t|a204>c|g261>a|g278>c,S93>T(+ - +)|c287>g,A96>G(- + -)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| a318>c,R106>S(- - -)| 317 (318) 218 (219) 200 18 (19) 6 12 (13) 3 4 4 0 1 (2) 0 0 1 3 2 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 2 1 2 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 30 21 18 3 1 2 0 0 0 0 0 0 0 1 1 1 0 0 117 114 106 8 4 4 0 2 2 0 1 0 0 0 2 1 0 0 5 (6) 5 5 0 (1) 0 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccgtcagtgatagttac atgagctgggtccgccaggctccagggaaggggctggagtgggtctcgatt atttataggggaggtaccaca tattatgccgactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctatatcttcaaatgaacaccctgagaggtgaggacacggctctatattactgt gcgagcgtcggagtgggagctacagtcgcctttcaatac ASVGVGATVAFQY IGHV3-66 IGHD1-26 IGHJ4 ggattcaccgtcagtgatagttac atgagctgggtccgccaggctccagggaaggggctggagtgggtctcgatt atttataggggaggtaccaca tattatgccgactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctatatcttcaaatgaacaccctgagaggtgaggacacggctctatattactgt gcgagcgtcggagtgggagctacagtcgcctttcaatac IGA2 18 210 11 7 8 13 3 5 5 1 5 7 JY8QFUQ01B9DAH 20678 productive Homsap IGHV4-4*07 F (see comment) 862 89.67 191/213 nt NA Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD3-22*01 F 2 10 7 22 10.7.22 [1.17.38.11] CARERAYDRNGFYYANRHNWLDTW in-frame - The submitted sequence and the closest germline V-GENE allele show different CDR1-IMGT amino acid lengths (10 AA in JY8QFUQ01B9DAH ; 8 AA in Homsap_IGHV4-4*07) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tctggtgcctccatcacatttggcagttactactggaattggatccggcagcccgccgggaagggactggagtgggtaggacgtatctatgcaagtgggaccacccattccaacccctctctcgagactcgagtcatcatgtcactagacacgtccaacaaccagatctccctgaagctgacctctgtgaccgccgcggacacggccgtgtattactgtgcgagagaaagggcctatgatagaaatggcttttactacgcgaaccgccacaactggctggacacctggggccagggaaccctggtcatcgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 100 100 [317] g83>c,G28>A(- + -)|a103>g,S35>G(+ + -)|t105>c,S35>G(+ + -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a157>g,I53>V(+ - +)|t159>a,I53>V(+ - +)|g162>a|a172>g,T58>A(- - -)|c174>a,T58>A(- - -)|g191>c,S64>T(+ - +)|a196>c,N66>H(- - -)|c198>t,N66>H(- - -)|a200>c,Y67>S(+ - -)|c210>t|a214>g,K72>E(+ - -)|g221>c,S74>T(+ - +)|c230>t,T77>I(- - -)|g238>c,V80>L(+ - +)|g252>c,K84>N(+ - -)|t259>a,F87>I(+ - -)|g275>c,S92>T(+ - +)| g83>c,G28>A(- + -)|a103>g,S35>G(+ + -)|t105>c,S35>G(+ + -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a157>g,I53>V(+ - +)|t159>a,I53>V(+ - +)|g162>a| a172>g,T58>A(- - -)|c174>a,T58>A(- - -)|g191>c,S64>T(+ - +)| a196>c,N66>H(- - -)|c198>t,N66>H(- - -)|a200>c,Y67>S(+ - -)|c210>t|a214>g,K72>E(+ - -)|g221>c,S74>T(+ - +)|c230>t,T77>I(- - -)|g238>c,V80>L(+ - +)|g252>c,K84>N(+ - -)|t259>a,F87>I(+ - -)|g275>c,S92>T(+ - +)| 320 227 205 22 2 20 4 2 4 1 2 1 0 2 6 0 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 1 0 0 1 0 0 0 0 1 0 0 0 51 51 46 5 1 4 1 2 1 0 0 0 0 1 0 0 0 0 30 21 18 3 0 3 1 0 0 0 0 1 0 0 1 0 0 0 117 114 103 11 1 10 1 0 3 0 2 0 0 1 4 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tct ggtgcctccatcacatttggcagttactac tggaattggatccggcagcccgccgggaagggactggagtgggtaggacgt atctatgcaagtgggaccacc cattccaacccctctctcgagactcgagtcatcatgtcactagacacgtccaacaaccagatctccctgaagctgacctctgtgaccgccgcggacacggccgtgtattactgt gcgagagaaagggcctatgatagaaatggcttttactacgcgaaccgccacaactggctggacacc ARERAYDRNGFYYANRHNWLDT IGHV4-4 IGHD3-22 IGHJ5 ggtgcctccatcacatttggcagttactac tggaattggatccggcagcccgccgggaagggactggagtgggtaggacgt atctatgcaagtgggaccacc cattccaacccctctctcgagactcgagtcatcatgtcactagacacgtccaacaaccagatctccctgaagctgacctctgtgaccgccgcggacacggccgtgtattactgt gcgagagaaagggcctatgatagaaatggcttttactacgcgaaccgccacaactggctggacacc IGG1 22 216 11 11 6 13 5 9 2 0 14 6 JY8QFUQ01B9L1U 10741 productive (see comment) Homsap IGHV2-5*02 F 1134 96.31 235/244 nt 95.49 233/244 nt Homsap IGHJ6*02 F (see comment) 184 77.42 48/62 nt Homsap IGHD4-17*01 F 3 10 7 13 10.7.13 [9.17.38.11] CAHFSVTTPYGMDVW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) in FR3-IMGT at codon 81 of V-REGION: 'G' (1 nucleotides at position 173 to 173 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 84 of V-REGION: 'A' (1 nucleotides at position 185 to 185 in the user submitted sequence), (cause frameshift); gaccctcacgctgacctgcaccttctctgggttctcactcaccactactggagtgggtgtgggctggatccgtcagcccccaggaaaggccctggagtggcttgcagtcattttttgggatgatgatgagcgccacagcccatctctgaggagaaggctcaccatcaccaagGgacatctccaaAaaaccaggtggtccttacaatgaccaacatggaccctgtggacacagccacatattactgtgcacacttttcggtgactaccccatatggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggctcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG2 100 100 [317] g92>c,S31>T(+ - +)|g104>c,S35>T(+ - +)|c163>g,L55>V(+ - +)|a170>t,Y57>F(- + -)|a193>g,K65>E(+ - -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c222>a,S74>R(- - -)|c245>t,T82>I(- - -)| g92>c,S31>T(+ - +)|g104>c,S35>T(+ - +)| c163>g,L55>V(+ - +)| a170>t,Y57>F(- + -)|a193>g,K65>E(+ - -)| t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c222>a,S74>R(- - -)|c245>t,T82>I(- - -)| a319>t,R107>F(- - -)|g320>t,R107>F(- - -)|a321>t,R107>F(- - -)|c322>t| 318 (322) 250 (254) 241 9 (13) 0 (1) 9 (12) 2 0 1 (2) 1 0 1 1 (3) 0 2 1 0 (1) 0 78 28 28 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 0 2 0 0 0 0 0 0 0 0 2 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 21 19 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 117 114 110 4 0 4 1 0 1 1 0 1 0 0 0 0 0 0 6 (10) 6 6 0 (4) 0 (1) 0 (3) 0 0 0 (1) 0 0 0 0 (2) 0 0 0 0 (1) 0 aa,13-14(FR1)|aa,40-41(FR1)|ta,102-103(CDR1)|aa,141-142(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,238-239(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,267-268(FR3)|aa,270-271(FR3)|aa,277-278(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,10-11(FR1)|tt,73-74(FR1)|tt,82-83(CDR1)|ta,102-103(CDR1)|tt,158-159(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,80-83(CDR1)|ggct,118-121(FR2)|ggct,155-158(FR2)|agca,220-223(FR3)|ggct,224-227(FR3)| aacc,41-44(FR1)|agcc,131-134(FR2)|agcc,202-205(FR3)|aacc,253-256(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)| gaccctcacgctgacctgcaccttctct gggttctcactcaccactactggagtgggt gtgggctggatccgtcagcccccaggaaaggccctggagtggcttgcagtc attttttgggatgatgatgag cgccacagcccatctctgaggagaaggctcaccatcaccaaggacatctccaaaaaccaggtggtccttacaatgaccaacatggaccctgtggacacagccacatattactgt gcacacttttcggtgactaccccatatggtatggacgtc AHFSVTTPYGMDV IGHV2-5 IGHD4-17 IGHJ6 gggttctcactcaccactactggagtgggt gtgggctggatccgtcagcccccaggaaaggccctggagtggcttgcagtc attttttgggatgatgatgag cgccacagcccatctctgaggagaaggctcaccatcaccaaggacatctccaaaaaccaggtggtccttacaatgaccaacatggaccctgtggacacagccacatattactgt gcacacttttcggtgactaccccatatggtatggacgtc IGG2 9 216 4 5 1 5 3 4 0 0 5 4 JY8QFUQ01B9LAE 22156 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 856 88.13 193/219 nt 87.67 192/219 nt Homsap IGHJ4*02 F 123 72.92 35/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [2.16.38.11] CTRDIGGRYSYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 43 of V-REGION: 1 nucleotides (from position 46 in the user submitted sequence), (cause frameshift) gcctccgggattcacctttagtagtttctggatgcactgggtccgcaagttccaggggagggactggtgtgggtcgcacggactaatgagtatgggagtatcacaaactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgtacaagggacatcggtgggcgctactcctactggggccagggagtcctggtctccgcctcatcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [280] a72>g|g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>P(+ - -)|t78>g,S26>P(+ - -)|c90>t|c108>t|a110>t,Y37>F(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g247>a,A83>T(- - -)|c257>g,T86>R(- - -)|g261>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|g313>a,A105>T(- - -)|a318>g| a72>g|g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>P(+ - -)|t78>g,S26>P(+ - -)| c90>t|c108>t|a110>t,Y37>F(- + -)| c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g247>a,A83>T(- - -)|c257>g,T86>R(- - -)|g261>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| g313>a,A105>T(- - -)|a318>g| 320 227 199 28 7 21 5 7 4 2 0 0 1 0 1 1 3 4 78 7 2 5 1 4 1 0 1 1 0 0 0 0 1 0 0 1 36 24 21 3 2 1 0 0 2 0 0 0 1 0 0 0 0 0 51 50 45 5 2 3 1 1 1 0 0 0 0 0 0 0 0 2 30 24 18 6 0 6 1 1 0 1 0 0 0 0 0 0 2 1 117 114 107 7 1 6 1 4 0 0 0 0 0 0 0 1 1 0 8 8 6 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctccg ggattcacctttagtagtttctgg atgcactgggtccgcaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgt acaagggacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttagtagtttctgg atgcactgggtccgcaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgt acaagggacatcggtgggcgctactcctac IGA2 21 212 13 8 9 13 4 8 3 2 9 7 JY8QFUQ01B9LAR 16994 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 954 93.55 203/217 nt 92.17 200/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [1.16.38.11] CVRAGFGELSGFDCW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 50 of V-REGION: 'G' (1 nucleotides at position 64 to 64 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 87 of V-REGION: 'C' (1 nucleotides at position 165 to 165 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 55 of V-REGION: 1 nucleotides (from position 79 in the user submitted sequence), (cause frameshift) cctctggattcacctttggttattatggcatgagctgggtccgccaagctccgggggaggggcGtggagtgggtctctgtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccCctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttcccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [291, 290] a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a138>g|a142>g,K48>E(+ - -)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a138>g|a142>g,K48>E(+ - -)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 224 (225) 209 15 (16) 3 (4) 12 3 3 1 1 1 (2) 0 2 0 2 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 50 48 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| cctct ggattcacctttggttattatggc atgagctgggtccgccaagctccgggggaggggctggagtgggtctctgt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgagctgggtccgccaagctccgggggaggggctggagtgggtctctgt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG2 14 212 7 7 3 7 4 7 3 0 7 4 JY8QFUQ01B9NGM 18240 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 984 93.72 209/223 nt NA Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD2-15*01 F 2 8 10 16 8.10.16 [1.17.38.11] CAADGCPGGTCYHGFDMW in-frame - ctctggattcactttcagtaacgtctggatgcactgggtccgccaggctccagggaaggggctggagtgggttggccgtattagaagcaaaactgctggtggggcaacagaatacgctgcgcccgtgagaggcatattctccatctcaagagatgattcaaagaacacgttgtatctgcaaatgaacagcctgaagaccgaggacacagccgtgtattactgtgccgcagatggttgtcctggtggtacctgctatcatggttttgatatgtggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [302, 301] c110>t,A37>V(+ - +)|a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)|a170>g,K57>R(+ + +)|a182>c,D61>A(- - -)|a190>g,T64>A(- - -)|c198>a,D66>E(+ - +)|a207>g|a215>g,K72>R(+ + +)|g224>t,R75>I(- + -)|a229>t,T77>S(+ - +)|a252>g|c259>t|a285>g|a313>g,T105>A(- - -)|a316>g,T106>A(- - -)| c110>t,A37>V(+ - +)| a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)| a170>g,K57>R(+ + +)|a182>c,D61>A(- - -)|a190>g,T64>A(- - -)| c198>a,D66>E(+ - +)|a207>g|a215>g,K72>R(+ + +)|g224>t,R75>I(- + -)|a229>t,T77>S(+ - +)|a252>g|c259>t|a285>g| a313>g,T105>A(- - -)|a316>g,T106>A(- - -)| 320 231 215 16 4 12 8 1 2 0 2 1 1 0 0 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 1 0 0 1 0 0 0 0 0 0 0 30 30 27 3 0 3 2 0 0 0 1 0 0 0 0 0 0 0 117 114 106 8 4 4 4 0 1 0 0 1 1 0 0 0 1 0 8 8 6 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| ctct ggattcactttcagtaacgtctgg atgcactgggtccgccaggctccagggaaggggctggagtgggttggccgt attagaagcaaaactgctggtggggcaaca gaatacgctgcgcccgtgagaggcatattctccatctcaagagatgattcaaagaacacgttgtatctgcaaatgaacagcctgaagaccgaggacacagccgtgtattactgt gccgcagatggttgtcctggtggtacctgctatcatggttttgatatg AADGCPGGTCYHGFDM IGHV3-15 IGHD2-15 IGHJ3 ggattcactttcagtaacgtctgg atgcactgggtccgccaggctccagggaaggggctggagtgggttggccgt attagaagcaaaactgctggtggggcaaca gaatacgctgcgcccgtgagaggcatattctccatctcaagagatgattcaaagaacacgttgtatctgcaaatgaacagcctgaagaccgaggacacagccgtgtattactgt gccgcagatggttgtcctggtggtacctgctatcatggttttgatatg IGA2 14 219 9 5 3 5 6 9 4 0 6 4 JY8QFUQ01B9QXU 20515 productive (see comment) Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F or Homsap IGHV4-34*12 F 835 88.26 188/213 nt 87.79 187/213 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-16*01 F 3 8 7 14 8.7.14 [1.17.37.11] CARKGLVPSMFFFDFW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 97 of V-REGION: 1 nucleotides (from position 192 in the user submitted sequence), (cause frameshift) ctctggtgggtccttcagtggttacttctggaactggatccgccagcccccagggaaggggccggagtggattggagaagtcagtcatgatggaagtaccaacttcaatccgtccctcaagagtcgagtctccatgtcagttgacacgatcaagaagcaggtcttcctgaaactgagctctgtgactgccgggacacggctatatattattgtgcgagaaagggacttgtgccatctatgttcttctttgacttctggggccagggatccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [287] a77>c,Y26>S(+ - -)|a113>t,Y38>F(- + -)|g119>a,S40>N(- - -)|t149>c,L50>P(- - -)|g162>a|a166>g,I56>V(+ - +)|a170>g,N57>S(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|c192>t|a200>t,Y67>F(- + -)|c204>t|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|a240>t|t247>a,S83>I(- - -)|c248>t,S83>I(- - -)|c255>g,N85>K(+ - -)|t259>g,F87>V(+ - -)|c263>t,S88>F(- - -)|g270>a|c285>t|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| a77>c,Y26>S(+ - -)| a113>t,Y38>F(- + -)| g119>a,S40>N(- - -)|t149>c,L50>P(- - -)|g162>a| a166>g,I56>V(+ - +)|a170>g,N57>S(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|c192>t| a200>t,Y67>F(- + -)|c204>t|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|a240>t|t247>a,S83>I(- - -)|c248>t,S83>I(- - -)|c255>g,N85>K(+ - -)|t259>g,F87>V(+ - -)|c263>t,S88>F(- - -)|g270>a|c285>t|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| g319>a|g320>a| 318 (320) 219 (221) 194 25 (27) 7 (9) 18 4 6 (8) 6 1 1 0 4 1 0 1 0 1 78 4 3 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 51 51 48 3 1 2 0 2 0 1 0 0 0 0 0 0 0 0 30 21 16 5 1 4 3 1 1 0 0 0 0 0 0 0 0 0 117 113 98 15 5 10 1 3 5 0 0 0 3 1 0 1 0 1 6 (8) 6 6 0 (2) 0 (2) 0 0 0 (2) 0 0 0 0 0 0 0 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtgggtccttcagtggttacttc tggaactggatccgccagcccccagggaaggggccggagtggattggagaa gtcagtcatgatggaagtacc aacttcaatccgtccctcaagagtcgagtctccatgtcagttgacacgatcaagaagcaggtcttcctgaaactgagctctgtgactgccgggacacggctatatattattgt gcgagaaagggacttgtgccatctatgttcttctttgacttc ARKGLVPSMFFFDF IGHV4-34 IGHD3-16 IGHJ4 ggtgggtccttcagtggttacttc tggaactggatccgccagcccccagggaaggggccggagtggattggagaa gtcagtcatgatggaagtacc aacttcaatccgtccctcaagagtcgagtctccatgtcagttgacacgatcaagaagcaggtcttcctgaaactgagctctgtgactgccgggacacggctatatattattgt gcgagaaagggacttgtgccatctatgttcttctttgacttc IGG1 24 209 17 7 12 13 5 11 6 1 12 5 JY8QFUQ01B9RA3 17048 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 940 93.06 201/216 nt 92.59 200/216 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 3 8 7 12 8.7.12 [1.17.38.11] CARDTGQWKDFDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 58 of V-REGION: 1 nucleotides (from position 87 in the user submitted sequence), (cause frameshift) ctctggattcatcttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgtagtaatacggggggactgacacagcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgtgcaagagatacggggcagtggaaggactttgactactggggccagggaaccctggtcaccgtctccttagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaaagatgggaacgtggtcgtcgcatgcctggtccaggggcttcttccccca NA IGA2 79 95 [283, 284] c86>t,T29>I(- - -)|c159>g|t167>g,I56>S(- - -)|t174>c|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| c86>t,T29>I(- - -)| c159>g| t167>g,I56>S(- - -)|t174>c|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| 320 224 209 15 4 11 3 1 1 1 1 0 0 0 2 3 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 30 23 16 7 1 6 2 1 0 1 0 0 0 0 1 0 0 2 117 114 108 6 2 4 1 0 0 0 1 0 0 0 1 2 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgt agtaatacggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac ARDTGQWKDFDY IGHV3-74 IGHD6-19 IGHJ4 ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgt agtaatacggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac IGA2 15 212 6 9 2 8 4 7 3 1 4 7 JY8QFUQ01B9S8H 12731 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 954 93.55 203/217 nt NA Homsap IGHJ2*01 F 247 96.23 51/53 nt Homsap IGHD2-21*01 F 1 8 8 12 8.8.12 [1.17.38.11] CAKVVWSLWYFDLW in-frame + ctctacattcgcctttagcaactatgccatgagctgggtccgccaggctccagggaagggactggagtgggtgtcagatattagtgggagtggtgttagcacacactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgtgcgaaagtagtctggagcctctggtacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [285] g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)|g147>a|c159>g|c164>a,A55>D(- - -)|t174>g|g188>t,G63>V(- - -)|t196>c,Y66>H(+ - -)|a204>g|a214>c,K72>Q(+ - -)|c249>g|a285>t,R95>S(- - -)| g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)| g147>a|c159>g|c164>a,A55>D(- - -)| t174>g|g188>t,G63>V(- - -)| t196>c,Y66>H(+ - -)|a204>g|a214>c,K72>Q(+ - -)|c249>g|a285>t,R95>S(- - -)| a320>t| 319 (320) 224 (225) 210 14 (15) 5 (6) 9 2 3 0 1 1 1 1 (2) 0 1 2 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 2 0 0 0 0 0 0 1 0 0 0 51 51 48 3 2 1 0 1 0 0 0 1 0 0 0 1 0 0 30 24 22 2 1 1 0 0 0 0 0 0 0 0 0 0 1 1 117 114 109 5 2 3 1 0 0 1 1 0 1 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct acattcgcctttagcaactatgcc atgagctgggtccgccaggctccagggaagggactggagtgggtgtcagat attagtgggagtggtgttagcaca cactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgt gcgaaagtagtctggagcctctggtacttcgatctc AKVVWSLWYFDL IGHV3-23 IGHD2-21 IGHJ2 acattcgcctttagcaactatgcc atgagctgggtccgccaggctccagggaagggactggagtgggtgtcagat attagtgggagtggtgttagcaca cactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgt gcgaaagtagtctggagcctctggtacttcgatctc IGG1 14 213 6 8 3 8 3 6 4 1 4 5 JY8QFUQ01B9TMK 13882 productive Homsap IGHV4-61*02 F 853 86.94 193/222 nt NA Homsap IGHJ5*02 F 192 86.27 44/51 nt Homsap IGHD5-24*01 ORF 1 10 7 14 10.7.14 [2.17.38.11] CARGMATIKTVRFDPW in-frame - gtctctggtggctccatgaggagtggaattaattcctggacatggatccggcagcccgccgggaagggactggagtggattgggcgtatctatatcaatggagacaccaactacaatccctccctcaagagtcgggccagcatatcaatggacacgtccaagaaccagttctccctgcacttgacctctgtgaccgccgcagattcggccgtctattattgcgcgagagggatggccacaataaaaacggtccgattcgacccctggggccagggaatcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [296] c90>g,I30>M(+ + -)|c93>g,S31>R(- - -)|t105>a|g107>t,S36>I(- - -)|t109>a,Y37>N(- - -)|c111>t,Y37>N(- - -)|a113>c,Y38>S(+ - -)|g119>c,S40>T(+ - +)|c120>a,S40>T(+ - +)|c173>t,T58>I(- - -)|g176>a,S59>N(- - -)|g189>a|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c204>t|a225>g|t227>c,V76>A(+ - +)|c230>g,T77>S(+ - +)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|a268>c,K90>H(- - +)|g270>c,K90>H(- - +)|c271>t|g275>c,S92>T(+ - +)|c294>t|a295>t,T99>S(+ - +)|g303>c|c309>t|t312>c| c90>g,I30>M(+ + -)|c93>g,S31>R(- - -)|t105>a|g107>t,S36>I(- - -)|t109>a,Y37>N(- - -)|c111>t,Y37>N(- - -)|a113>c,Y38>S(+ - -)| g119>c,S40>T(+ - +)|c120>a,S40>T(+ - +)| c173>t,T58>I(- - -)|g176>a,S59>N(- - -)|g189>a|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| c204>t|a225>g|t227>c,V76>A(+ - +)|c230>g,T77>S(+ - +)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|a268>c,K90>H(- - +)|g270>c,K90>H(- - +)|c271>t|g275>c,S92>T(+ - +)|c294>t|a295>t,T99>S(+ - +)|g303>c|c309>t|t312>c| a320>g| 319 (320) 229 (230) 200 29 (30) 9 (10) 20 3 (4) 4 6 2 2 1 1 2 4 3 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 23 7 1 6 0 0 1 0 1 0 0 2 0 2 1 0 51 51 49 2 0 2 0 0 0 0 0 1 0 0 1 0 0 0 30 21 16 5 1 4 1 3 1 0 0 0 0 0 0 0 0 0 117 114 99 15 7 8 2 1 4 2 1 0 1 0 3 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct ggtggctccatgaggagtggaattaattcc tggacatggatccggcagcccgccgggaagggactggagtggattgggcgt atctatatcaatggagacacc aactacaatccctccctcaagagtcgggccagcatatcaatggacacgtccaagaaccagttctccctgcacttgacctctgtgaccgccgcagattcggccgtctattattgc gcgagagggatggccacaataaaaacggtccgattcgacccc ARGMATIKTVRFDP IGHV4-61 IGHD5-24 IGHJ5 ggtggctccatgaggagtggaattaattcc tggacatggatccggcagcccgccgggaagggactggagtggattgggcgt atctatatcaatggagacacc aactacaatccctccctcaagagtcgggccagcatatcaatggacacgtccaagaaccagttctccctgcacttgacctctgtgaccgccgcagattcggccgtctattattgc gcgagagggatggccacaataaaaacggtccgattcgacccc IGG1 29 216 15 14 10 19 5 10 7 2 10 10 JY8QFUQ01B9UZ4 21320 productive Homsap IGHV1-18*01 F 957 94.39 202/214 nt NA Homsap IGHJ5*02 F 237 96.08 49/51 nt Homsap IGHD1-20*01 F 1 8 8 11 8.8.11 [0.17.38.11] CARVIRTNWLDPW in-frame - tggttacacctttactacctatggcatcagctgggtgcgacaggcccctggacaagggcctgagtgggtgggatggatcagcgcttacaatggaaatacaaactctgcacagaagttccaggacagagtcaccctgaccacagacacatccacgagcacagcctacatggagctgaggaacctgagatctgacgacacggccgtgtattactgtgcgagagtaattcggaccaactggctcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [278] c105>t|g107>c,S36>T(+ - +)|t114>c|t149>c,L50>P(- - -)|a157>g,M53>V(+ - -)|t189>a|c192>t|a200>c,Y67>S(+ - -)|c211>t,L71>F(+ - -)|g221>a,G74>D(- - -)|a232>c,M78>L(+ + -)|g278>a,S93>N(- - -)| c105>t|g107>c,S36>T(+ - +)|t114>c| t149>c,L50>P(- - -)|a157>g,M53>V(+ - -)| t189>a|c192>t| a200>c,Y67>S(+ - -)|c211>t,L71>F(+ - -)|g221>a,G74>D(- - -)|a232>c,M78>L(+ + -)|g278>a,S93>N(- - -)| a320>t| 319 (320) 221 (222) 209 12 (13) 4 (5) 8 1 2 3 2 2 0 0 (1) 1 1 0 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 1 1 0 0 0 0 1 0 0 0 51 51 49 2 0 2 1 0 0 1 0 0 0 0 0 0 0 0 30 24 22 2 2 0 0 0 1 0 0 0 0 1 0 0 0 0 117 114 109 5 0 5 0 2 1 0 2 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|ta,175-176(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,5-6(FR1)|tt,75-76(FR1)|tt,81-82(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|tt,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,3-6(FR1)|agct,8-11(FR1)|agct,24-27(FR1)|ggct,72-75(FR1)|ggtt,79-82(CDR1)|agct,106-109(CDR1)|ggta,112-115(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)| agct,8-11(FR1)|agct,24-27(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|agct,209-212(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggttacacctttactacctatggc atcagctgggtgcgacaggcccctggacaagggcctgagtgggtgggatgg atcagcgcttacaatggaaataca aactctgcacagaagttccaggacagagtcaccctgaccacagacacatccacgagcacagcctacatggagctgaggaacctgagatctgacgacacggccgtgtattactgt gcgagagtaattcggaccaactggctcgacccc ARVIRTNWLDP IGHV1-18 IGHD1-20 IGHJ5 ggttacacctttactacctatggc atcagctgggtgcgacaggcccctggacaagggcctgagtgggtgggatgg atcagcgcttacaatggaaataca aactctgcacagaagttccaggacagagtcaccctgaccacagacacatccacgagcacagcctacatggagctgaggaacctgagatctgacgacacggccgtgtattactgt gcgagagtaattcggaccaactggctcgacccc IGA1 12 213 8 4 5 6 3 6 0 4 7 1 JY8QFUQ01B9XU2 11198 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*02 F or Homsap IGHV4-39*05 F 974 93.67 207/221 nt NA Homsap IGHJ1*01 F (see comment) 170 80.77 42/52 nt Homsap IGHD5-18*01 F 3 10 7 14 10.7.14 [1.17.38.11] CARSQSRYSWGDFLHW in-frame + Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) tctctggtgactccatcagcagtactagttactactggggctggatccgccagcccccagggagggggctggagtggattggaagcatctattatagtgggagcacctcctacaacccgtccctcaagagtcgaatcaccatgtccatagacatgtccaagaaccaattctccctgaggctgagctctgtgaccgccgcagacacggctgtttatttctgtgcgcggtcccagagtagatacagttggggtgacttcttacactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttc NA IGA1 100 100 [294] g83>a,G28>D(- - -)|g104>c,S35>T(+ - +)|a143>g,K48>R(+ + +)|g162>a|t165>c|a197>c,Y66>S(+ - -)|g226>a,V76>I(+ - +)|a234>g,I78>M(+ + -)|g238>a,V80>I(+ - +)|c245>t,T82>M(- - -)|g258>a|a269>g,K90>R(+ + +)|g303>t|a308>t,Y103>F(- + -)| g83>a,G28>D(- - -)|g104>c,S35>T(+ - +)| a143>g,K48>R(+ + +)|g162>a|t165>c| a197>c,Y66>S(+ - -)|g226>a,V76>I(+ - +)|a234>g,I78>M(+ + -)|g238>a,V80>I(+ - +)|c245>t,T82>M(- - -)|g258>a|a269>g,K90>R(+ + +)|g303>t|a308>t,Y103>F(- + -)| a316>c|a318>g|c319>t|a320>c| 315 (320) 224 (229) 210 (211) 14 (18) 4 (8) 10 3 (4) 5 1 (2) 1 1 (3) 0 1 0 1 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 51 51 48 3 2 1 1 1 0 1 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 105 9 2 7 2 3 1 0 1 0 1 0 0 0 1 0 3 (8) 3 3 (4) 0 (4) 0 (4) 0 0 (1) 0 0 (1) 0 0 (2) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatcagcagtactagttactac tggggctggatccgccagcccccagggagggggctggagtggattggaagc atctattatagtgggagcacc tcctacaacccgtccctcaagagtcgaatcaccatgtccatagacatgtccaagaaccaattctccctgaggctgagctctgtgaccgccgcagacacggctgtttatttctgt gcgcggtcccagagtagatacagttggggtgacttcttacac ARSQSRYSWGDFLH IGHV4-39 IGHD5-18 IGHJ1 ggtgactccatcagcagtactagttactac tggggctggatccgccagcccccagggagggggctggagtggattggaagc atctattatagtgggagcacc tcctacaacccgtccctcaagagtcgaatcaccatgtccatagacatgtccaagaaccaattctccctgaggctgagctctgtgaccgccgcagacacggctgtttatttctgt gcgcggtcccagagtagatacagttggggtgacttcttacac IGA1 14 216 10 4 6 8 4 6 4 0 8 2 JY8QFUQ01BAB5T 22104 productive (see comment) Homsap IGHV3-72*01 F 1043 96.43 216/224 nt 95.98 215/224 nt Homsap IGHJ6*02 F 229 85.48 53/62 nt Homsap IGHD4-11*01 ORF 2 8 10 13 8.10.13 [1.17.38.11] CVRGLHSNYYGMDVR in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 41 of V-REGION: 'G' (1 nucleotides at position 37 to 37 in the user submitted sequence), (cause frameshift); cctctggattcaccttcagtgaccactacatggactGgggtccgccaggctccagggaaggggctggagtgggttggccgtagtagagacaaagttaacagttacagcacagaatacgccgcgtctgcgaagggcagattcaccatctcaagagatgaatcaaagaactcactgtatctgcaaatgaacagcctgaaaagcgaggacacggccgtgtattactgtgttagaggcctacacagtaattactacggtatggacgtcaggggccaagggaccacggtcaccgtctcctcagcatccccgaccagcccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 95 [296, 295] c167>g,T56>S(+ - +)|a172>g,N58>D(+ + -)|c179>t,A60>V(+ - +)|c191>g,T64>S(+ - +)|t212>c,V71>A(+ - +)|a216>g|t246>a,D82>E(+ - +)|c287>g,T96>S(+ - +)|c314>t,A105>V(+ - +)| c167>g,T56>S(+ - +)|a172>g,N58>D(+ + -)|c179>t,A60>V(+ - +)|c191>g,T64>S(+ - +)| t212>c,V71>A(+ - +)|a216>g|t246>a,D82>E(+ - +)|c287>g,T96>S(+ - +)| c314>t,A105>V(+ - +)|a320>g| 319 (320) 231 (232) 222 9 (10) 1 (2) 8 2 (3) 0 2 1 0 0 0 1 0 3 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 26 4 0 4 1 0 1 0 0 0 0 0 0 2 0 0 117 114 110 4 1 3 1 0 0 1 0 0 0 1 0 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,165-168(FR2-CDR2)|agct,177-180(CDR2)|aact,253-256(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tgct,312-315(FR3)| cctct ggattcaccttcagtgaccactac atggactgggtccgccaggctccagggaaggggctggagtgggttggccgt agtagagacaaagttaacagttacagcaca gaatacgccgcgtctgcgaagggcagattcaccatctcaagagatgaatcaaagaactcactgtatctgcaaatgaacagcctgaaaagcgaggacacggccgtgtattactgt gttagaggcctacacagtaattactacggtatggacgtc VRGLHSNYYGMDV IGHV3-72 IGHD4-11 IGHJ6 ggattcaccttcagtgaccactac atggactgggtccgccaggctccagggaaggggctggagtgggttggccgt agtagagacaaagttaacagttacagcaca gaatacgccgcgtctgcgaagggcagattcaccatctcaagagatgaatcaaagaactcactgtatctgcaaatgaacagcctgaaaagcgaggacacggccgtgtattactgt gttagaggcctacacagtaattactacggtatggacgtc IGA2 8 219 4 4 1 4 3 4 1 0 3 4 JY8QFUQ01BACNA 2275 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 967 94.44 204/216 nt 93.98 203/216 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-8*01 F 2 8 7 8 8.7.8 [1.17.38.11] CARDNYGDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 63 of V-REGION: 1 nucleotides (from position 95 in the user submitted sequence), (cause frameshift) ctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgtgttaatggtgatggggtaggacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcacagtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatggaacgtggtcgtcgcatgcctggtccagggcttcttcccccag NA IGA2 100 89 [271, 270] g153>t|a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|c192>g,S64>R(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g153>t| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|c192>g,S64>R(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 224 212 12 5 7 3 2 0 0 0 0 0 1 3 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 30 23 20 3 0 3 2 0 0 0 0 0 0 0 0 1 0 0 117 114 106 8 4 4 1 2 0 0 0 0 0 1 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgt gttaatggtgatggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgt gttaatggtgatggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac IGA2 12 212 5 7 2 8 3 4 5 0 4 3 JY8QFUQ01BAGKP 16679 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 812 85.52 189/221 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD6-13*01 F 3 10 7 16 10.7.16 [1.17.38.11] CARRWARQRLVMDYFDYW in-frame + tctctggtgactccatcagtagtactcattactattggggctggatccggcagcccccagggaggggactggagtgggttgggagtatccactacactgggagcacctactacaactggtccctcaagcatcgagtctctatatcggtggacacatcgagtaaccagttctccctgaggttgaggtctgtgaccgccgctgacacggctgtatactactgtgcgagacgatgggcgaggcaacggctggtcatggactactttgactactggggccagggaatcgtggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG2 100 100 [301] g83>a,G28>D(- - -)|c93>t|g104>c,S35>T(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|c114>t|c129>g|a143>g,K48>R(+ + +)|g147>a|a157>g,I53>V(+ - +)|t169>c,Y57>H(+ - -)|t171>c,Y57>H(+ - -)|t174>c|g176>c,S59>T(+ - +)|c205>t,P69>W(- - -)|c206>g,P69>W(- - -)|a220>c,S74>H(+ - -)|g221>a,S74>H(+ - -)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|c237>g|a240>g|g246>a|c249>g|a251>g,K84>S(- - -)|g252>t,K84>S(- - -)|a269>g,K90>R(+ + +)|c271>t|c276>g,S92>R(- - -)|a291>t|g303>a|t306>c| g83>a,G28>D(- - -)|c93>t|g104>c,S35>T(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|c114>t| c129>g|a143>g,K48>R(+ + +)|g147>a|a157>g,I53>V(+ - +)| t169>c,Y57>H(+ - -)|t171>c,Y57>H(+ - -)|t174>c|g176>c,S59>T(+ - +)| c205>t,P69>W(- - -)|c206>g,P69>W(- - -)|a220>c,S74>H(+ - -)|g221>a,S74>H(+ - -)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|c237>g|a240>g|g246>a|c249>g|a251>g,K84>S(- - -)|g252>t,K84>S(- - -)|a269>g,K90>R(+ + +)|c271>t|c276>g,S92>R(- - -)|a291>t|g303>a|t306>c| a320>g| 319 (320) 228 (229) 196 32 (33) 13 (14) 19 5 (6) 6 5 4 2 0 2 0 2 5 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 2 4 0 2 2 0 1 0 0 0 1 0 0 0 51 51 47 4 2 2 2 1 0 0 0 0 0 0 0 1 0 0 30 21 17 4 1 3 0 0 0 3 0 0 0 0 1 0 0 0 117 114 96 18 8 10 3 3 3 1 1 0 2 0 0 4 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatcagtagtactcattactat tggggctggatccggcagcccccagggaggggactggagtgggttgggagt atccactacactgggagcacc tactacaactggtccctcaagcatcgagtctctatatcggtggacacatcgagtaaccagttctccctgaggttgaggtctgtgaccgccgctgacacggctgtatactactgt gcgagacgatgggcgaggcaacggctggtcatggactactttgactac ARRWARQRLVMDYFDY IGHV4-39 IGHD6-13 IGHJ4 ggtgactccatcagtagtactcattactat tggggctggatccggcagcccccagggaggggactggagtgggttgggagt atccactacactgggagcacc tactacaactggtccctcaagcatcgagtctctatatcggtggacacatcgagtaaccagttctccctgaggttgaggtctgtgaccgccgctgacacggctgtatactactgt gcgagacgatgggcgaggcaacggctggtcatggactactttgactac IGG2 32 216 20 12 11 19 9 13 10 3 12 7 JY8QFUQ01BAGOW 13762 productive (see comment) Homsap IGHV6-1*01 F, or Homsap IGHV6-1*02 F 928 89.91 205/228 nt 89.47 204/228 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD1-7*01 F 3 10 9 19 10.9.19 [2.17.38.11] CARSSRRDRLSDRNYYELDFW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 72 of V-REGION: 'A' (1 nucleotides at position 133 to 133 in the user submitted sequence), (cause frameshift); tctccgggggacagtgtctctagcaacagtgccacttggaactggatcaggcagtccccaacgggaggccttgagtggctgggaaggacatactacaggtccaaatgggatactgattatgcggcgtctgtgAaaaagtcgaataaccctcaccccagacacatccaagaaccagttctccctgcaattgaactccgttagtcccgaggacacggctgtgtattattgtgcaagatcttccagacgcgatcgtttgtctgataggaactactatgagctggacttctggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 100 100 [317] a73>t,I25>S(- - -)|t74>c,I25>S(- - -)|c75>t,I25>S(- - -)|t76>c,S26>P(+ - -)|c78>g,S26>P(+ - -)|t111>c|g112>a,A38>T(- - -)|t139>a,S47>T(+ - +)|a142>g,R48>G(- - -)|g186>a|t190>g,Y64>D(- - -)|a194>c,N65>T(- + -)|a204>g|t206>c,V69>A(+ - +)|a207>g,V69>A(+ - +)|a232>c,I78>L(+ + +)|a236>c,N79>T(- + -)|g270>a|c271>t|t279>c|g282>t|c284>g,T95>S(+ - +)|c309>t| a73>t,I25>S(- - -)|t74>c,I25>S(- - -)|c75>t,I25>S(- - -)|t76>c,S26>P(+ - -)|c78>g,S26>P(+ - -)| t111>c|g112>a,A38>T(- - -)| t139>a,S47>T(+ - +)|a142>g,R48>G(- - -)| g186>a|t190>g,Y64>D(- - -)|a194>c,N65>T(- + -)| a204>g|t206>c,V69>A(+ - +)|a207>g,V69>A(+ - +)|a232>c,I78>L(+ + +)|a236>c,N79>T(- + -)|g270>a|c271>t|t279>c|g282>t|c284>g,T95>S(+ - +)|c309>t| g319>t|a320>c| 318 (320) 234 (236) 211 23 (25) 8 (10) 15 3 3 3 5 3 (4) 0 1 1 0 2 1 (2) 1 78 6 1 5 0 5 0 0 1 2 0 0 1 0 0 1 0 0 36 30 28 2 1 1 0 1 0 1 0 0 0 0 0 0 0 0 51 51 49 2 0 2 1 0 0 0 0 0 0 1 0 0 0 0 30 27 24 3 1 2 0 1 0 0 1 0 0 0 0 0 0 1 117 114 103 11 6 5 2 1 2 2 2 0 0 0 0 1 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,5-6(FR1)|aa,40-41(FR1)|aa,103-104(CDR1)|aa,118-119(FR2)|aa,162-163(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,184-185(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|ta,206-207(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,227-228(FR3)|aa,235-236(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,5-6(FR1)|tt,114-115(CDR1)|tt,149-150(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|tt,198-199(FR3)|ta,206-207(FR3)|ta,227-228(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggta,3-6(FR1)|agct,8-11(FR1)|agca,14-17(FR1)|agca,100-103(CDR1)|ggca,128-131(FR2)|ggct,155-158(FR2)|ggta,188-191(CDR2)|agta,204-207(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tgcc,69-72(FR1)|tgct,108-111(CDR1)|aact,118-121(FR2)|tact,169-172(CDR2)|aacc,228-231(FR3)|aacc,235-238(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|aact,274-277(FR3)|tact,307-310(FR3)| tctccg ggggacagtgtctctagcaacagtgccact tggaactggatcaggcagtccccaacgggaggccttgagtggctgggaagg acatactacaggtccaaatgggatact gattatgcggcgtctgtgaaaagtcgaataaccctcaccccagacacatccaagaaccagttctccctgcaattgaactccgttagtcccgaggacacggctgtgtattattgt gcaagatcttccagacgcgatcgtttgtctgataggaactactatgagctggacttc ARSSRRDRLSDRNYYELDF IGHV6-1 IGHD1-7 IGHJ4 ggggacagtgtctctagcaacagtgccact tggaactggatcaggcagtccccaacgggaggccttgagtggctgggaagg acatactacaggtccaaatgggatact gattatgcggcgtctgtgaaaagtcgaataaccctcaccccagacacatccaagaaccagttctccctgcaattgaactccgttagtcccgaggacacggctgtgtattattgt gcaagatcttccagacgcgatcgtttgtctgataggaactactatgagctggacttc IGA2 18 222 11 7 5 7 6 11 6 2 7 3 JY8QFUQ01BAKGF 9539 productive Homsap IGHV4-61*01 F, or Homsap IGHV4-61*03 F 848 87.33 193/221 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-19*01 F 1 10 7 12 10.7.12 [1.17.38.11] CARGGGWTPYFDSW in-frame - tctctggtggctccgtcagcagtggtaattactactggaactggatccgccaacccccagggaagggactggagtggattggatatatctactatgctggggccaccaacgtcgccccctccctcaagaaccgagtcaccataacgagagacacgtccaagaaccaattttccctgaggttgacttctgtgaccgctgcggacacggccgtatattactgtgcgaggggcggtggctggaccccctactttgactcctggggccagggaaatctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [288] g107>a,S36>N(- - -)|g119>a,S40>N(- - -)|g129>c|g132>a|g162>a|t171>c|c174>t|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|t199>g,Y67>V(- - -)|a200>t,Y67>V(- - -)|a202>g,N68>A(- - -)|a203>c,N68>A(- - -)|g221>a,S74>N(- - -)|t222>c,S74>N(- - -)|t235>a,S79>T(+ - +)|a237>g,S79>T(+ - +)|g238>a,V80>R(- - -)|t239>g,V80>R(- - -)|g258>a|c261>t|a269>g,K90>R(+ + +)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g303>a| g107>a,S36>N(- - -)| g119>a,S40>N(- - -)|g129>c|g132>a|g162>a| t171>c|c174>t|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| t199>g,Y67>V(- - -)|a200>t,Y67>V(- - -)|a202>g,N68>A(- - -)|a203>c,N68>A(- - -)|g221>a,S74>N(- - -)|t222>c,S74>N(- - -)|t235>a,S79>T(+ - +)|a237>g,S79>T(+ - +)|g238>a,V80>R(- - -)|t239>g,V80>R(- - -)|g258>a|c261>t|a269>g,K90>R(+ + +)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g303>a| a318>g|a320>g| 317 (320) 226 (229) 198 (199) 28 (30) 9 (11) 19 5 (7) 8 4 2 1 0 1 1 4 0 0 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 29 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 47 4 3 1 0 3 0 0 0 0 0 0 1 0 0 0 30 21 15 6 2 4 2 0 1 1 0 0 0 0 2 0 0 0 117 114 97 17 4 13 3 4 3 1 1 0 1 1 1 0 0 2 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcagcagtggtaattactac tggaactggatccgccaacccccagggaagggactggagtggattggatat atctactatgctggggccacc aacgtcgccccctccctcaagaaccgagtcaccataacgagagacacgtccaagaaccaattttccctgaggttgacttctgtgaccgctgcggacacggccgtatattactgt gcgaggggcggtggctggaccccctactttgactcc ARGGGWTPYFDS IGHV4-61 IGHD6-19 IGHJ4 ggtggctccgtcagcagtggtaattactac tggaactggatccgccaacccccagggaagggactggagtggattggatat atctactatgctggggccacc aacgtcgccccctccctcaagaaccgagtcaccataacgagagacacgtccaagaaccaattttccctgaggttgacttctgtgaccgctgcggacacggccgtatattactgt gcgaggggcggtggctggaccccctactttgactcc IGA1 28 216 19 9 12 16 7 12 7 2 14 5 JY8QFUQ01BAR1C 7904 productive Homsap IGHV3-9*01 F 891 90.32 196/217 nt NA Homsap IGHJ6*02 F (see comment) 193 79.03 49/62 nt Homsap IGHD3-22*01 F 2 8 8 26 8.8.26 [1.17.38.11] CVKDHGVTYFYDSVGYVSGAYNYAMDVW in-frame + Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) ctctggattcacctttgatgattttggcatgcactgggtccggcaagttccagggaagggcccggagtgggtcgcaggtattagttggaatggtggacatatggactatgcggactctgtgaagggccgattcaccgtgtcccgagacaacgccgagaattccctgtatctgcaaatgaacagtctgacacctgaagacacggccctgtattactgtgtaaaagaccatggcgtcacttacttttatgacagtgttggttatgtctccggggcgtacaactacgcaatggacgtctggggccaggggacgacggtcaccgtctcctcagcctccaccaaggggccccatcggtcttcccctggcaccctcctccaagagcacctctgggggcacagcggcccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 88 [328, 329] a110>t,Y37>F(- + -)|c113>g,A38>G(- + -)|c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)|t160>g,S54>A(- + -)|a184>g,S62>G(+ + -)|t189>a|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|c192>t,S64>H(+ - -)|a195>g,I65>M(+ + -)|g197>a,G66>D(- - -)|a232>g,I78>V(+ - +)|c234>g,I78>V(+ - +)|a238>c|a250>g,K84>E(+ - -)|c255>t|g284>c,R95>T(- - -)|g286>c,A96>P(- - -)|g291>a|t301>c|c314>t,A105>V(+ - +)| a110>t,Y37>F(- + -)|c113>g,A38>G(- + -)| c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)|t160>g,S54>A(- + -)| a184>g,S62>G(+ + -)|t189>a|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|c192>t,S64>H(+ - -)|a195>g,I65>M(+ + -)| g197>a,G66>D(- - -)|a232>g,I78>V(+ - +)|c234>g,I78>V(+ - +)|a238>c|a250>g,K84>E(+ - -)|c255>t|g284>c,R95>T(- - -)|g286>c,A96>P(- - -)|g291>a|t301>c| c314>t,A105>V(+ - +)|t321>c|a322>c| 320 (322) 225 (227) 203 22 (24) 5 (7) 17 4 3 4 2 (3) 2 (3) 0 1 1 2 2 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 51 51 48 3 0 3 0 0 1 1 0 0 0 0 0 0 0 1 30 24 18 6 1 5 2 1 1 0 1 0 0 1 0 0 0 0 117 114 104 10 4 6 2 2 1 1 1 0 0 0 2 1 0 0 8 (10) 8 7 1 (3) 0 (2) 1 0 0 1 0 (1) 0 (1) 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttgatgattttggc atgcactgggtccggcaagttccagggaagggcccggagtgggtcgcaggt attagttggaatggtggacatatg gactatgcggactctgtgaagggccgattcaccgtgtcccgagacaacgccgagaattccctgtatctgcaaatgaacagtctgacacctgaagacacggccctgtattactgt gtaaaagaccatggcgtcacttacttttatgacagtgttggttatgtctccggggcgtacaactacgcaatggacgtc VKDHGVTYFYDSVGYVSGAYNYAMDV IGHV3-9 IGHD3-22 IGHJ6 ggattcacctttgatgattttggc atgcactgggtccggcaagttccagggaagggcccggagtgggtcgcaggt attagttggaatggtggacatatg gactatgcggactctgtgaagggccgattcaccgtgtcccgagacaacgccgagaattccctgtatctgcaaatgaacagtctgacacctgaagacacggccctgtattactgt gtaaaagaccatggcgtcacttacttttatgacagtgttggttatgtctccggggcgtacaactacgcaatggacgtc IGG1 21 213 12 9 6 10 6 11 4 1 9 7 JY8QFUQ01BB73D 9004 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F (see comment) 714 81.78 175/214 nt NA Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD6-6*01 F 3 8 7 9 8.7.9 [1.17.38.11] CARLDGHFDLW in-frame + Low V-REGION identity (81,78% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctgggttcaccatcactcactactccatggcctgggtccgccaggctccggggaaggggctggagtgggtctcattaatttatcccggcgggagcacattccacgcagactccgtgagggggcgcctcatcatctccagagacgaatccaagagtgagctgtatcttcggatgacgaaagtgaaagccgaagacgcggccgtatattactgtgcgagactcgacgggcacttcgatctctggggccgtggcaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 93 100 [272, 273] g88>a,V30>I(+ - +)|g104>c,S35>T(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a138>g|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g|a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c222>g|a225>c|t226>c,F76>L(+ - -)|c230>t,T77>I(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|a275>c,N92>T(- + -)|c276>g,N92>T(- + -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|g291>a|a295>g,T99>A(- - -)|g303>a| g88>a,V30>I(+ - +)|g104>c,S35>T(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a138>g|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g| a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c222>g|a225>c|t226>c,F76>L(+ - -)|c230>t,T77>I(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|a275>c,N92>T(- + -)|c276>g,N92>T(- + -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|g291>a|a295>g,T99>A(- - -)|g303>a| g319>c|a320>t| 318 (320) 220 (222) 181 39 (41) 7 (9) 32 9 6 2 3 5 2 2 (3) 2 3 (4) 3 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 2 0 0 2 0 1 0 1 0 0 0 51 51 46 5 1 4 2 0 0 0 0 0 0 1 1 0 1 0 30 21 17 4 2 2 0 0 0 1 1 0 0 0 1 0 0 1 117 114 90 24 4 20 7 4 2 2 2 2 1 1 0 3 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcaccatcactcactactcc atggcctgggtccgccaggctccggggaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgagggggcgcctcatcatctccagagacgaatccaagagtgagctgtatcttcggatgacgaaagtgaaagccgaagacgcggccgtatattactgt gcgagactcgacgggcacttcgatctc ARLDGHFDL IGHV3-53 IGHD6-6 IGHJ2 gggttcaccatcactcactactcc atggcctgggtccgccaggctccggggaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgagggggcgcctcatcatctccagagacgaatccaagagtgagctgtatcttcggatgacgaaagtgaaagccgaagacgcggccgtatattactgt gcgagactcgacgggcacttcgatctc IGA2 39 210 20 19 8 17 12 22 5 2 24 8 JY8QFUQ01BB93X 13677 productive (see comment) Homsap IGHV1-2*04 F 925 92.96 198/213 nt 92.49 197/213 nt Homsap IGHJ3*02 F 196 88 44/50 nt Homsap IGHD5-18*01 F 1 8 8 14 8.8.14 [0.17.37.11] CTRDTSLDNGAALDIW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 74 of V-REGION: 1 nucleotides (from position 122 in the user submitted sequence), (cause frameshift) tggatacaccttcaccgactactatatgcactgggtgcgacaggccccaggacaagggcttgagtggatgggatggatcaaccctaaaagtggcggcacaaattatgcacggaagtttcaggctgggtcaccatgaccagggacacatccatcaacactgtctacatggagttgagcaggttgaaatctgacgacacggccatttattactgtacgagggatacttctttggacaacggagccgctcttgatatttggggccaagggacaaaggtcaccgtctcttcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [287] g107>a,G36>D(- - -)|t138>a|c177>a,N59>K(+ - -)|t189>c|c198>t|a206>g,Q69>R(+ - -)|g246>a|g254>a,S85>N(- - -)|a258>t|c260>t,A87>V(+ - +)|c271>t|c280>t|g284>a,R95>K(+ + +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|g313>a,A105>T(- - -)|a318>g| g107>a,G36>D(- - -)| t138>a| c177>a,N59>K(+ - -)|t189>c| c198>t|a206>g,Q69>R(+ - -)|g246>a|g254>a,S85>N(- - -)|a258>t|c260>t,A87>V(+ - +)|c271>t|c280>t|g284>a,R95>K(+ + +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| g313>a,A105>T(- - -)|a318>g| 320 221 204 17 8 9 2 6 4 1 0 1 1 1 0 0 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 24 22 2 1 1 0 0 0 1 0 1 0 0 0 0 0 0 117 113 102 11 5 6 1 4 4 0 0 0 1 0 0 0 1 0 8 8 6 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggct,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatacaccttcaccgactactat atgcactgggtgcgacaggccccaggacaagggcttgagtggatgggatgg atcaaccctaaaagtggcggcaca aattatgcacggaagtttcaggctgggtcaccatgaccagggacacatccatcaacactgtctacatggagttgagcaggttgaaatctgacgacacggccatttattactgt acgagggatacttctttggacaacggagccgctcttgatatt TRDTSLDNGAALDI IGHV1-2 IGHD5-18 IGHJ3 ggatacaccttcaccgactactat atgcactgggtgcgacaggccccaggacaagggcttgagtggatgggatgg atcaaccctaaaagtggcggcaca aattatgcacggaagtttcaggctgggtcaccatgaccagggacacatccatcaacactgtctacatggagttgagcaggttgaaatctgacgacacggccatttattactgt acgagggatacttctttggacaacggagccgctcttgatatt IGG1 15 212 11 4 9 11 2 4 6 1 6 2 JY8QFUQ01BB9AK 16135 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 836 87.91 189/215 nt 86.98 187/215 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-21*02 F 3 8 7 15 8.7.15 [1.17.38.11] CARGRTVVTVIDYIDYW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'A' (1 nucleotides at position 11 to 11 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 45 of V-REGION: 'A' (1 nucleotides at position 49 to 49 in the user submitted sequence), (cause frameshift); tctctgggggActccattagtggttactattggacgtggatccggcagAcccccagggaagggactggagtggattggaaatgtccattacactgggagtaccaagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgtgcgagaggacgaacggtggtgactgttattgactacattgactattggggtcagggagccctggtcagcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [293] t81>g|c90>t|a106>g,S36>G(+ + -)|c114>t|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|g162>a|t163>a,Y55>N(- - -)|a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|c192>t|c198>g,N66>K(+ - -)|a203>g,N68>S(- - -)|a234>g,I78>M(+ + -)|a240>t|c245>t,T82>M(- - -)|a251>g,K84>R(+ + +)|t262>a,S88>T(+ - +)|g267>c|g270>a|c271>t|g275>c,S92>T(+ - +)|g282>a|a283>g,T95>A(- - -)|g303>c| t81>g|c90>t|a106>g,S36>G(+ + -)|c114>t| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|g162>a|t163>a,Y55>N(- - -)| a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|c192>t| c198>g,N66>K(+ - -)|a203>g,N68>S(- - -)|a234>g,I78>M(+ + -)|a240>t|c245>t,T82>M(- - -)|a251>g,K84>R(+ + +)|t262>a,S88>T(+ - +)|g267>c|g270>a|c271>t|g275>c,S92>T(+ - +)|g282>a|a283>g,T95>A(- - -)|g303>c| a320>g| 319 (320) 222 (223) 196 26 (27) 11 (12) 15 6 (7) 3 5 1 0 0 1 2 5 2 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 3 1 1 0 2 0 0 0 0 0 0 0 0 1 51 51 47 4 1 3 0 1 0 0 0 0 0 1 1 1 0 0 30 21 17 4 1 3 1 0 1 1 0 0 0 0 1 0 0 0 117 114 100 14 6 8 4 2 2 0 0 0 1 1 3 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct gggggctccattagtggttactat tggacgtggatccggcagcccccagggaagggactggagtggattggaaat gtccattacactgggagtacc aagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttattgactacattgactat ARGRTVVTVIDYIDY IGHV4-59 IGHD2-21 IGHJ4 gggggctccattagtggttactat tggacgtggatccggcagcccccagggaagggactggagtggattggaaat gtccattacactgggagtacc aagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttattgactacattgactat IGA2 26 210 15 11 8 15 7 11 7 4 11 4 JY8QFUQ01BBDL1 15508 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 882 89.86 195/217 nt 89.4 194/217 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD6-13*01 F 2 8 8 12 8.8.12 [1.17.37.11] CVREGAATGSFYYW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 84 of V-REGION: 1 nucleotides (from position 156 in the user submitted sequence), (cause frameshift) cctctggattcaccttcagtgactatgccatgagctgggtccgccaggctccagcgaaggggctggaatgggtctcagcgattagcagtagtggtgatagaacatactacgcagactccgtgaagggccgattcaccatctccagagacagttccaggggactctgtatttgcaaatgaaccgcctgagcgccgaggacacggccctatatttctgtgtgagagaaggagcagcaactggttcgttttactactggggccagggagccctggtcaccgtctcctcatcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagcagatgggaacgtggtcatcgcctgcctgtccagggcttcttccccca NA IGA1 71 95 [284, 283] t90>c|c105>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g140>c,G47>A(- + -)|g153>a|t165>g|t171>c|g172>a,G58>S(+ + -)|g188>a,G63>D(- - -)|c192>a,S64>R(- - -)|g225>a|a245>g,N82>S(- - -)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|g258>t|c265>t|a277>c,S93>R(- - -)|a285>c,R95>S(- - -)|g301>c,V101>L(+ - +)|a308>t,Y103>F(- + -)|c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)| t90>c|c105>t|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g140>c,G47>A(- + -)|g153>a|t165>g| t171>c|g172>a,G58>S(+ + -)|g188>a,G63>D(- - -)|c192>a,S64>R(- - -)| g225>a|a245>g,N82>S(- - -)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|g258>t|c265>t|a277>c,S93>R(- - -)|a285>c,R95>S(- - -)|g301>c,V101>L(+ - +)|a308>t,Y103>F(- + -)| c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)| 320 225 201 24 8 16 5 5 3 2 2 1 1 0 2 1 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 1 1 1 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 0 0 0 0 0 0 1 0 0 1 30 24 20 4 1 3 0 2 0 1 0 1 0 0 0 0 0 0 117 113 102 11 3 8 3 1 1 0 2 0 1 0 1 1 1 0 8 8 6 2 0 2 1 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtgactatgcc atgagctgggtccgccaggctccagcgaaggggctggaatgggtctcagcg attagcagtagtggtgatagaaca tactacgcagactccgtgaagggccgattcaccatctccagagacagttccaggggactctgtatttgcaaatgaaccgcctgagcgccgaggacacggccctatatttctgt gtgagagaaggagcagcaactggttcgttttactac VREGAATGSFYY IGHV3-23 IGHD6-13 IGHJ4 ggattcaccttcagtgactatgcc atgagctgggtccgccaggctccagcgaaggggctggaatgggtctcagcg attagcagtagtggtgatagaaca tactacgcagactccgtgaagggccgattcaccatctccagagacagttccaggggactctgtatttgcaaatgaaccgcctgagcgccgaggacacggccctatatttctgt gtgagagaaggagcagcaactggttcgttttactac IGA1 22 212 13 9 7 12 6 10 5 3 9 5 JY8QFUQ01BBLQU 7243 productive Homsap IGHV3-11*01 F 954 93.55 203/217 nt NA Homsap IGHJ2*01 F (see comment) 211 88.68 47/53 nt Homsap IGHD2-8*02 F 3 8 8 12 8.8.12 [1.17.38.11] CADIRLGDWCFDIW in-frame + Other possibilities: Homsap_IGHJ1*01 (highest number of consecutive identical nucleotides) ctctggattcaccttcagtgaccactacatgagttggatccgccaggctccagggaaggggctggagtggatttcatatattagtactagtggtaatatgatttattacgcagactctgtgaagggccgattcaccgtctccagggacaacgccaagaactcactgtatctgcagatgaacagcctgagagccgaggacacggccgtctatttctgtgccgatataagactgggtgactggtgcttcgatatctggggccggggcaccctggtcaatgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] t109>c,Y37>H(+ - -)|c120>t|g157>a,V53>I(+ - +)|c165>t|g173>c,S58>T(+ - +)|g188>a,S63>N(- - -)|c191>t,T64>M(- - -)|c192>g,T64>M(- - -)|a195>t|c198>t|a232>g,I78>V(+ - +)|a270>g|g303>c|a308>t,Y103>F(- + -)| t109>c,Y37>H(+ - -)| c120>t|g157>a,V53>I(+ - +)|c165>t| g173>c,S58>T(+ - +)|g188>a,S63>N(- - -)|c191>t,T64>M(- - -)|c192>g,T64>M(- - -)|a195>t| c198>t|a232>g,I78>V(+ - +)|a270>g|g303>c|a308>t,Y103>F(- + -)| g315>c|a316>g,R106>D(+ - -)|g317>a,R106>D(+ - -)|a318>t,R106>D(+ - -)|g319>a|a320>t| 314 (320) 219 (225) 205 14 (20) 6 (9) 8 (11) 2 (3) 2 (4) 4 1 0 0 2 (4) 0 2 (3) 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 2 0 0 0 0 0 0 0 0 0 30 24 19 5 1 4 0 1 1 0 0 0 1 0 1 1 0 0 117 114 109 5 3 2 2 0 1 0 0 0 1 0 1 0 0 0 2 (8) 2 2 0 (6) 0 (3) 0 (3) 0 (1) 0 (2) 0 0 0 0 0 (2) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgaccactac atgagttggatccgccaggctccagggaaggggctggagtggatttcatat attagtactagtggtaatatgatt tattacgcagactctgtgaagggccgattcaccgtctccagggacaacgccaagaactcactgtatctgcagatgaacagcctgagagccgaggacacggccgtctatttctgt gccgatataagactgggtgactggtgcttcgatatc ADIRLGDWCFDI IGHV3-11 IGHD2-8 IGHJ2 ggattcaccttcagtgaccactac atgagttggatccgccaggctccagggaaggggctggagtggatttcatat attagtactagtggtaatatgatt tattacgcagactctgtgaagggccgattcaccgtctccagggacaacgccaagaactcactgtatctgcagatgaacagcctgagagccgaggacacggccgtctatttctgt gccgatataagactgggtgactggtgcttcgatatc IGA1 14 213 9 5 6 9 3 5 5 1 3 5 JY8QFUQ01BBQG8 4136 productive Homsap IGHV4-4*07 F 953 93.95 202/215 nt NA Homsap IGHJ2*01 F 247 96.23 51/53 nt NA 8 7 9 8.7.9 [1.17.38.11] CARGDWYFDLW in-frame + tctctggtggctccatcattaatcactactggagttggatccggcagcccgccgggaagggactggagtggattgggcgtagtcataccagtggaagcaccaggtacaacccctccctcaagagtcgagtcaccgtgtcagcagacacgtccaagaaccagttctccctgaagcttagctctgtgaccgccgcggacacggccgtgtattactgtgcgagaggagactggtacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [274] g104>t,S35>I(- - -)|g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)|c120>t|t167>g,I56>S(- - -)|c168>t,I56>S(- - -)|t169>c,Y57>H(+ - -)|g189>a|a197>g,N66>R(+ - -)|c198>g,N66>R(+ - -)|a232>g,M78>V(+ - -)|t239>c,V80>A(+ - +)|g273>t| g104>t,S35>I(- - -)|g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)| c120>t| t167>g,I56>S(- - -)|c168>t,I56>S(- - -)|t169>c,Y57>H(+ - -)|g189>a| a197>g,N66>R(+ - -)|c198>g,N66>R(+ - -)|a232>g,M78>V(+ - -)|t239>c,V80>A(+ - +)|g273>t| a320>g| 319 (320) 222 (223) 209 13 (14) 3 (4) 10 2 (3) 2 2 3 0 0 0 0 0 1 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 1 0 0 0 0 0 0 1 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 21 17 4 1 3 0 1 1 1 0 0 0 0 0 0 0 1 117 114 109 5 1 4 2 0 0 1 0 0 0 0 0 1 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcattaatcactac tggagttggatccggcagcccgccgggaagggactggagtggattgggcgt agtcataccagtggaagcacc aggtacaacccctccctcaagagtcgagtcaccgtgtcagcagacacgtccaagaaccagttctccctgaagcttagctctgtgaccgccgcggacacggccgtgtattactgt gcgagaggagactggtacttcgatctc ARGDWYFDL IGHV4-4 IGHJ2 ggtggctccatcattaatcactac tggagttggatccggcagcccgccgggaagggactggagtggattgggcgt agtcataccagtggaagcacc aggtacaacccctccctcaagagtcgagtcaccgtgtcagcagacacgtccaagaaccagttctccctgaagcttagctctgtgaccgccgcggacacggccgtgtattactgt gcgagaggagactggtacttcgatctc IGG1 13 210 9 4 4 7 5 6 2 1 4 6 JY8QFUQ01BBSRC 17458 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 812 85.52 189/221 nt 85.07 188/221 nt Homsap IGHJ3*01 F (see comment) 178 84 42/50 nt Homsap IGHD3-16*01 F 2 10 7 12 10.7.12 [1.17.38.11] CARTYTYRNAFDVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT at codon 34 of V-REGION: 'ACC' (3 nucleotides at position 21 to 23 in the user submitted sequence), (do not cause frameshift); tctttggtgactctctgagtACCagtagcagtttctactggggctgggtccgccaggccccagggaagggactggagtgggttgggactgtttattatagtgggagcgcccactacaatccgtccctcaagagtcgagtcgccatatccgtggacacggcccaaaaccaggtctccctgactttgaactctgtgaccgccgcagacgccgctgtctatttctgtgcgagaacttatacttatagaaatgcttttgacgtctggggccaggggacattggtcgctgtgtcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgaggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [291, 292] c77>t,S26>F(- - -)|g83>a,G28>D(- - -)|c87>t|a88>c,I30>L(+ + +)|c90>g,I30>L(+ + +)|c93>t|t105>c|a110>t,Y37>F(- + -)|a124>g,I42>V(+ - +)|c133>g,P45>A(- - -)|g147>a|a157>g,I53>V(+ - +)|g164>c,S55>T(+ - +)|a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|a193>g,T65>A(- - -)|t196>c,Y66>H(+ - -)|c204>t|a229>g,T77>A(- - -)|a240>g|t247>g,S83>A(- + -)|a250>c,K84>Q(+ - -)|g252>a,K84>Q(+ - -)|t259>g,F87>V(+ - -)|a269>c,K90>T(- - -)|g270>t,K90>T(- - -)|c271>t|g275>a,S92>N(- - -)|a295>g,T99>A(- - -)|g297>c,T99>A(- - -)|g303>c|a308>t,Y103>F(- + -)| c77>t,S26>F(- - -)| g83>a,G28>D(- - -)|c87>t|a88>c,I30>L(+ + +)|c90>g,I30>L(+ + +)|c93>t|t105>c|a110>t,Y37>F(- + -)| a124>g,I42>V(+ - +)|c133>g,P45>A(- - -)|g147>a|a157>g,I53>V(+ - +)|g164>c,S55>T(+ - +)| a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|a193>g,T65>A(- - -)| t196>c,Y66>H(+ - -)|c204>t|a229>g,T77>A(- - -)|a240>g|t247>g,S83>A(- + -)|a250>c,K84>Q(+ - -)|g252>a,K84>Q(+ - -)|t259>g,F87>V(+ - -)|a269>c,K90>T(- - -)|g270>t,K90>T(- - -)|c271>t|g275>a,S92>N(- - -)|a295>g,T99>A(- - -)|g297>c,T99>A(- - -)|g303>c|a308>t,Y103>F(- + -)| c319>a|a320>c| 318 (320) 227 (229) 195 32 (34) 8 (10) 24 7 4 6 2 3 (4) 0 (1) 2 0 3 2 1 2 78 5 4 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 36 30 23 7 3 4 0 1 2 1 1 0 1 0 0 1 0 0 51 51 46 5 1 4 2 1 0 0 0 0 0 0 1 1 0 0 30 21 18 3 0 3 2 0 1 0 0 0 0 0 0 0 0 0 117 114 98 16 4 12 3 2 2 1 2 0 1 0 2 0 1 2 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 (1) 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tcttt ggtgactctctgagtagtagcagtttctac tggggctgggtccgccaggccccagggaagggactggagtgggttgggact gtttattatagtgggagcgcc cactacaatccgtccctcaagagtcgagtcgccatatccgtggacacggcccaaaaccaggtctccctgactttgaactctgtgaccgccgcagacgccgctgtctatttctgt gcgagaacttatacttatagaaatgcttttgacgtc ARTYTYRNAFDV IGHV4-39 IGHD3-16 IGHJ3 ggtgactctctgagtagtagcagtttctac tggggctgggtccgccaggccccagggaagggactggagtgggttgggact gtttattatagtgggagcgcc cactacaatccgtccctcaagagtcgagtcgccatatccgtggacacggcccaaaaccaggtctccctgactttgaactctgtgaccgccgcagacgccgctgtctatttctgt gcgagaacttatacttatagaaatgcttttgacgtc IGA1 31 216 18 13 9 15 9 16 5 3 16 7 JY8QFUQ01BBTVQ 4144 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 865 88.58 194/219 nt 87.67 192/219 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [2.16.38.11] CVRAGFGELSGFDCW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'C' (1 nucleotides at position 17 to 17 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 40 of V-REGION: 2 nucleotides (from position 37 in the user submitted sequence), (cause frameshift) ggcctcctggattcacCctttggttattatggcatgactggtccgccaactccgggggagggggctggagtgggtctctggtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [291] c71>g|a72>g|g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>P(+ - -)|a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|t121>c,W41>L(+ - -)|g122>t,W41>L(+ - -)|g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|t135>c,A45>L(+ - +)|c137>g,P46>R(- - -)|a138>g,P46>R(- - -)|a143>g,K48>R(+ + +)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| c71>g|a72>g|g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>P(+ - -)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| t121>c,W41>L(+ - -)|g122>t,W41>L(+ - -)|g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|t135>c,A45>L(+ - +)|c137>g,P46>R(- - -)|a138>g,P46>R(- - -)|a143>g,K48>R(+ + +)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 226 (227) 200 26 (27) 4 (5) 22 4 3 3 4 1 (2) 0 2 0 4 3 2 0 78 8 3 5 2 3 1 0 1 1 0 0 0 0 1 1 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 49 41 8 0 8 2 0 1 2 0 0 0 0 1 1 1 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ggcctcct ggattcacctttggttattatggc atgactggtccgccaactccgggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgactggtccgccaactccgggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 20 211 10 10 4 11 6 9 2 0 14 4 JY8QFUQ01BBVFC 1451 productive (see comment) Homsap IGHV3-48*01 F, or Homsap IGHV3-48*03 F 801 85.71 186/217 nt 85.25 185/217 nt Homsap IGHJ4*02 F (see comment) 186 87.5 42/48 nt Homsap IGHD1-1*01 F 3 8 8 10 8.8.10 [1.17.38.11] CVRDLNWSFDSW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 44 of V-REGION: 'C' (1 nucleotides at position 45 to 45 in the user submitted sequence), (cause frameshift); ctctggattcacctttactagttacagtttcaactgggtccgccCaggctccagggaaggcgctggagtggatttcatacatcactatcaatggtaatgacaagttctacgcaggctctgtgaagggccgattcgccgtctccagagacgatgccaagaattctctgtatctgcaaatgagcagcctgagagccgaagacacgggtgtttattactgtgtgagggacctcaactggtcttttgactcctggggccagggaaccctggtcaccgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [279] c90>t|g104>c,S35>T(+ - +)|c108>t|t111>c|c114>t|a115>t,M39>F(+ - -)|g117>c,M39>F(+ - -)|g146>c,G49>A(- + -)|g157>a,V53>I(+ - +)|t168>c|g170>c,S57>T(+ - +)|g173>t,S58>I(- - -)|t174>c,S58>I(- - -)|g176>a,S59>N(- - -)|a184>g,S62>G(+ + -)|g188>a,S63>N(- - -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|t194>a,I65>K(- + -)|a195>g,I65>K(- + -)|a197>t,Y66>F(- + -)|a206>g,D69>G(- - -)|a229>g,T77>A(- - -)|a232>g,I78>V(+ - +)|a244>g,N82>D(+ + -)|c255>t|a258>t|a275>g,N92>S(- - -)|g291>a|c299>g,A100>G(- + -)|g303>t|c314>t,A105>V(+ - +)|a318>g| c90>t|g104>c,S35>T(+ - +)|c108>t|t111>c|c114>t| a115>t,M39>F(+ - -)|g117>c,M39>F(+ - -)|g146>c,G49>A(- + -)|g157>a,V53>I(+ - +)| t168>c|g170>c,S57>T(+ - +)|g173>t,S58>I(- - -)|t174>c,S58>I(- - -)|g176>a,S59>N(- - -)|a184>g,S62>G(+ + -)|g188>a,S63>N(- - -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|t194>a,I65>K(- + -)|a195>g,I65>K(- + -)| a197>t,Y66>F(- + -)|a206>g,D69>G(- - -)|a229>g,T77>A(- - -)|a232>g,I78>V(+ - +)|a244>g,N82>D(+ + -)|c255>t|a258>t|a275>g,N92>S(- - -)|g291>a|c299>g,A100>G(- + -)|g303>t| c314>t,A105>V(+ - +)|a318>g| 320 225 192 33 10 23 9 4 5 3 0 1 3 1 4 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 4 1 0 0 3 1 0 0 0 0 1 0 0 0 51 51 47 4 0 4 0 1 0 0 0 0 1 0 2 0 0 0 30 24 13 11 1 10 3 2 0 2 0 1 0 1 1 0 1 0 117 114 103 11 4 7 5 1 1 0 0 0 2 0 0 1 1 0 8 8 6 2 1 1 1 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttactagttacagt ttcaactgggtccgccaggctccagggaaggcgctggagtggatttcatac atcactatcaatggtaatgacaag ttctacgcaggctctgtgaagggccgattcgccgtctccagagacgatgccaagaattctctgtatctgcaaatgagcagcctgagagccgaagacacgggtgtttattactgt gtgagggacctcaactggtcttttgactcc VRDLNWSFDS IGHV3-48 IGHD1-1 IGHJ4 ggattcacctttactagttacagt ttcaactgggtccgccaggctccagggaaggcgctggagtggatttcatac atcactatcaatggtaatgacaag ttctacgcaggctctgtgaagggccgattcgccgtctccagagacgatgccaagaattctctgtatctgcaaatgagcagcctgagagccgaagacacgggtgtttattactgt gtgagggacctcaactggtcttttgactcc IGA1 31 213 19 12 8 16 11 15 4 5 11 11 JY8QFUQ01BBZEX 20368 productive (see comment) Homsap IGHV3-7*01 F 892 89.95 197/219 nt 89.04 195/219 nt Homsap IGHJ3*02 F 196 88 44/50 nt Homsap IGHD1-1*01 F 3 8 8 10 8.8.10 [2.17.38.11] CARVNKDSTDMW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'G' (1 nucleotides at position 60 to 60 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 57 of V-REGION: 'A' (1 nucleotides at position 87 to 87 in the user submitted sequence), (cause frameshift); gcctctggattcacctttagtgactattggatgaggtggttccgccagcctccaggaagGggggctggagtgggtggccagcataaAaagaagatggaagtgagaaaggttatgtggactctgtgaagggccgcttcaccatcgccagagacaacgcccagaaatcactgtttttgcagatgaacagcctgagaggcgaggacacggctgtgtatttctgtgcgagagtcaacaaggactctactgatatgtggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccc NA IGA1 100 100 [282] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c120>g,S40>R(- - -)|g124>t,V42>F(+ - -)|g133>c,A45>P(- - -)|g141>a|a143>g,K48>R(+ + +)|a164>g,N55>S(- - -)|g171>a|c172>g,Q58>E(+ + -)|t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>t,Y66>G(+ - -)|a225>c|t235>g,S79>A(- + -)|a250>c,K84>Q(+ - -)|c255>a,N85>K(+ - -)|a263>t,Y88>F(- + -)|c265>t|a270>g|c287>g,A96>G(- + -)|a308>t,Y103>F(- + -)| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| c120>g,S40>R(- - -)|g124>t,V42>F(+ - -)|g133>c,A45>P(- - -)|g141>a|a143>g,K48>R(+ + +)|a164>g,N55>S(- - -)| g171>a|c172>g,Q58>E(+ + -)| t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>t,Y66>G(+ - -)|a225>c|t235>g,S79>A(- + -)|a250>c,K84>Q(+ - -)|c255>a,N85>K(+ - -)|a263>t,Y88>F(- + -)|c265>t|a270>g|c287>g,A96>G(- + -)|a308>t,Y103>F(- + -)| a320>t| 319 (320) 226 (227) 204 22 (23) 5 (6) 17 5 3 2 0 2 1 2 (3) 0 1 3 1 2 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 45 6 1 5 2 1 0 0 0 0 0 0 1 1 1 0 30 24 22 2 1 1 0 1 0 0 0 0 0 0 0 1 0 0 117 114 102 12 3 9 2 0 2 0 2 1 2 0 0 1 0 2 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacctttagtgactattgg atgaggtggttccgccagcctccaggaagggggctggagtgggtggccagc ataaaagaagatggaagtgagaaa ggttatgtggactctgtgaagggccgcttcaccatcgccagagacaacgcccagaaatcactgtttttgcagatgaacagcctgagaggcgaggacacggctgtgtatttctgt gcgagagtcaacaaggactctactgatatg ARVNKDSTDM IGHV3-7 IGHD1-1 IGHJ3 ggattcacctttagtgactattgg atgaggtggttccgccagcctccaggaagggggctggagtgggtggccagc ataaaagaagatggaagtgagaaa ggttatgtggactctgtgaagggccgcttcaccatcgccagagacaacgcccagaaatcactgtttttgcagatgaacagcctgagaggcgaggacacggctgtgtatttctgt gcgagagtcaacaaggactctactgatatg IGA1 22 213 10 12 5 11 5 11 4 1 14 3 JY8QFUQ01BC153 7583 productive (see comment) Homsap IGHV3-7*01 F 985 95.37 206/216 nt 94.44 204/216 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-15*01 F 2 7 8 11 7.8.11 [1.17.38.11] CARSGSGSHLDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 30 of V-REGION: 1 nucleotides (from position 16 in the user submitted sequence), (cause frameshift)in CDR1-IMGT, from codon 37 of V-REGION: 1 nucleotides (from position 24 in the user submitted sequence), (cause frameshift) tctctggattcaccttagtaggtttggatgacctgggtccgccagggtccagggaaggggctggagtgggtggccaacataaagcaagttggaaatgagagatactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcattgtatctgcaaatgaacagcctgagagtcgacgacacggctgtgtattactgtgcgagaagtggtagtgggagccacttagattactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcccctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 71 89 [280] c74>t|c108>g,S36>R(- - -)|g119>c,S40>T(+ - +)|c134>g,A45>G(- + -)|a176>t,D59>V(- - -)|g188>a,S63>N(- - -)|a194>g,K65>R(+ + +)|c259>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| c74>t| c108>g,S36>R(- - -)| g119>c,S40>T(+ - +)|c134>g,A45>G(- + -)| a176>t,D59>V(- - -)|g188>a,S63>N(- - -)|a194>g,K65>R(+ + +)| c259>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| g319>a|a320>g| 318 (320) 222 (224) 212 10 (12) 2 (4) 8 1 (2) 1 (2) 3 0 0 0 1 0 2 2 0 0 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 22 21 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 49 2 0 2 0 0 0 0 0 0 0 0 1 1 0 0 30 24 21 3 0 3 1 1 0 0 0 0 1 0 0 0 0 0 117 114 111 3 1 2 0 0 2 0 0 0 0 0 1 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tctct ggattcaccttagtaggtttgg atgacctgggtccgccagggtccagggaaggggctggagtgggtggccaac ataaagcaagttggaaatgagaga tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcattgtatctgcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaagtggtagtgggagccacttagattac ARSGSGSHLDY IGHV3-7 IGHD2-15 IGHJ4 ggattcaccttagtaggtttgg atgacctgggtccgccagggtccagggaaggggctggagtgggtggccaac ataaagcaagttggaaatgagaga tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcattgtatctgcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaagtggtagtgggagccacttagattac IGA1 9 211 4 5 3 7 1 2 1 0 4 4 JY8QFUQ01BCH7Y 13089 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 973 94.06 206/219 nt NA Homsap IGHJ2*01 F 247 96.23 51/53 nt Homsap IGHD2-21*01 F 1 8 8 12 8.8.12 [2.17.38.11] CAKVVWSLWYFDLW in-frame - gcctctacattcgcctttagcaactatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtgtcagatattagtgggagtggtgttagcacacactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgtgcgaaagtagtctggagcctctggtacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [287] g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)|c159>g|c164>a,A55>D(- - -)|t174>g|g188>t,G63>V(- - -)|t196>c,Y66>H(+ - -)|a204>g|a214>c,K72>Q(+ - -)|c249>g|a285>t,R95>S(- - -)| g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)| c159>g|c164>a,A55>D(- - -)| t174>g|g188>t,G63>V(- - -)| t196>c,Y66>H(+ - -)|a204>g|a214>c,K72>Q(+ - -)|c249>g|a285>t,R95>S(- - -)| a320>t| 319 (320) 226 (227) 213 13 (14) 4 (5) 9 2 2 0 1 1 1 1 (2) 0 1 2 1 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 2 0 0 0 0 0 0 1 0 0 0 51 51 49 2 1 1 0 0 0 0 0 1 0 0 0 1 0 0 30 24 22 2 1 1 0 0 0 0 0 0 0 0 0 0 1 1 117 114 109 5 2 3 1 0 0 1 1 0 1 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct acattcgcctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtgtcagat attagtgggagtggtgttagcaca cactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgt gcgaaagtagtctggagcctctggtacttcgatctc AKVVWSLWYFDL IGHV3-23 IGHD2-21 IGHJ2 acattcgcctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtgtcagat attagtgggagtggtgttagcaca cactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgt gcgaaagtagtctggagcctctggtacttcgatctc IGG2 13 213 5 8 2 7 3 6 3 1 4 5 JY8QFUQ01BCMPS 7729 productive (see comment) Homsap IGHV4-59*03 F 930 92.99 199/214 nt 92.52 198/214 nt Homsap IGHJ5*02 F (see comment) 201 88.24 45/51 nt Homsap IGHD3-22*01 F 2 8 7 16 8.7.16 [1.17.38.11] CARGHFDSSGYSNPLDHW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in CDR2-IMGT at codon 59 of V-REGION: 'AGCGGG' (6 nucleotides at position 89 to 94 in the user submitted sequence), (do not cause frameshift); ctctggtgtctccatcagtagtttctactggagttggatgaggcagcccccagggaggggactggagtggattggatatgtccatggcAGCGGGagtgggagcaccaactccaacccctccctcaagagtcgagtcaccatgtcagtggacacgtccaagaaccaattctccctgaagctgggctctgtgaccgctgcggacacggccgtgtattactgtgcgagaggacattttgatagtagtggttattccaaccctttagaccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagcccctaaggtcttcccgctgagcctcgacagcaccccccaagatggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA2 71 79 [300, 299] g83>t,G28>V(- - -)|a110>t,Y37>F(- + -)|c120>t|c126>g,I42>M(+ + -)|c127>a|a143>g,K48>R(+ + +)|g162>a|a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|t172>g,Y58>G(+ - -)|a173>g,Y58>G(+ - -)|a200>c,Y67>S(+ - -)|a234>g,I78>M(+ + -)|a240>g|a274>g,S92>G(+ + -)| g83>t,G28>V(- - -)|a110>t,Y37>F(- + -)| c120>t|c126>g,I42>M(+ + -)|c127>a|a143>g,K48>R(+ + +)|g162>a| a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|t172>g,Y58>G(+ - -)|a173>g,Y58>G(+ - -)| a200>c,Y67>S(+ - -)|a234>g,I78>M(+ + -)|a240>g|a274>g,S92>G(+ + -)| 315 217 202 15 4 11 6 1 1 1 1 1 1 0 0 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 0 0 1 0 51 51 46 5 3 2 1 1 1 0 0 1 0 0 0 1 0 0 30 21 17 4 0 4 2 0 0 1 0 0 0 0 0 0 0 1 117 114 110 4 1 3 3 0 0 0 1 0 0 0 0 0 0 0 3 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,257-258(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtgtctccatcagtagtttctac tggagttggatgaggcagcccccagggaggggactggagtggattggatat gtccatggcagtgggagcacc aactccaacccctccctcaagagtcgagtcaccatgtcagtggacacgtccaagaaccaattctccctgaagctgggctctgtgaccgctgcggacacggccgtgtattactgt gcgagaggacattttgatagtagtggttattccaaccctttagaccac ARGHFDSSGYSNPLDH IGHV4-59 IGHD3-22 IGHJ5 ggtgtctccatcagtagtttctac tggagttggatgaggcagcccccagggaggggactggagtggattggatat gtccatggcagtgggagcacc aactccaacccctccctcaagagtcgagtcaccatgtcagtggacacgtccaagaaccaattctccctgaagctgggctctgtgaccgctgcggacacggccgtgtattactgt gcgagaggacattttgatagtagtggttattccaaccctttagaccac IGA2 15 210 9 6 2 5 7 10 4 0 5 6 JY8QFUQ01BCV43 7656 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 963 94.01 204/217 nt 93.55 203/217 nt Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD6-6*01 F 2 8 8 16 8.8.16 [1.17.38.11] CAFSRALTGKWIDYLDHW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 57 to 57 in the user submitted sequence), (cause frameshift); ctctggattcatcttcagcaactactggatgcactgggtccgccaagctccagggaAaggggctggtgtgggtctcacgtattactggtgatgggagtaacccaatctacgcggaccccgtgaagggtcgattcaccatctccagagacaacgccaagaacacactatatctgcaaatgaacagtctgagagtcgaggacacggctgtgtattactgtgcctttagtagagcactcacggggaagtggatcgactaccttgaccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [297, 296] c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)|a170>c,N57>T(- + -)|a172>g,S58>G(+ + -)|g191>a,S64>N(- - -)|a193>c,T65>P(+ + -)|g197>t,S66>I(- - -)|t208>c,S70>P(+ - -)|c222>t|g258>a|g261>a|c287>t,A96>V(+ - +)| c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)| a170>c,N57>T(- + -)|a172>g,S58>G(+ + -)|g191>a,S64>N(- - -)|a193>c,T65>P(+ + -)| g197>t,S66>I(- - -)|t208>c,S70>P(+ - -)|c222>t|g258>a|g261>a|c287>t,A96>V(+ - +)| a315>c|a316>t,R106>F(- - -)|g317>t,R106>F(- - -)|a318>t,R106>F(- - -)|g319>a|a320>g| 314 (320) 219 (225) 206 13 (19) 4 (7) 9 (12) 1 (2) 4 (5) 3 2 2 (3) 0 0 (2) 0 0 0 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 1 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 1 0 0 2 0 0 0 0 0 0 0 117 114 108 6 3 3 0 2 2 1 0 0 0 0 0 0 1 0 2 (8) 2 2 0 (6) 0 (3) 0 (3) 0 (1) 0 (1) 0 0 0 (1) 0 0 (2) 0 0 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagcaactactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attactggtgatgggagtaaccca atctacgcggaccccgtgaagggtcgattcaccatctccagagacaacgccaagaacacactatatctgcaaatgaacagtctgagagtcgaggacacggctgtgtattactgt gcctttagtagagcactcacggggaagtggatcgactaccttgaccac AFSRALTGKWIDYLDH IGHV3-74 IGHD6-6 IGHJ4 ggattcatcttcagcaactactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attactggtgatgggagtaaccca atctacgcggaccccgtgaagggtcgattcaccatctccagagacaacgccaagaacacactatatctgcaaatgaacagtctgagagtcgaggacacggctgtgtattactgt gcctttagtagagcactcacggggaagtggatcgactaccttgaccac IGA2 13 213 10 3 7 8 3 5 3 1 3 6 JY8QFUQ01BD04C 22212 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKGLYYYDSSGYRVFDYW in-frame + ctctggattcacctttagcaactttgccatgagctgggtccgccaggctccagggaagggactggagtgggtctcgactattagtcctagtggtggtaccacatattacgcagactccgtgaagggccggttcaccatctccagagacagttccaagaacacgctgtatctgcaaatgaacatcctgagagccgaggacacggccacatattactgtgcgaaaggactatattattatgacagtagtggttatcgggtctttgactactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [299] g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)|g147>a|a162>g|g163>a,A55>T(- - -)|g172>c,G58>P(+ - -)|g173>c,G58>P(+ - -)|g191>c,S64>T(+ - +)|c198>t|a245>g,N82>S(- - -)|g278>t,S93>I(- - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)| g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)| g147>a|a162>g|g163>a,A55>T(- - -)| g172>c,G58>P(+ - -)|g173>c,G58>P(+ - -)|g191>c,S64>T(+ - +)| c198>t|a245>g,N82>S(- - -)|g278>t,S93>I(- - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)| a320>g| 319 (320) 224 (225) 211 13 (14) 3 (4) 10 2 (3) 4 1 1 0 0 1 0 3 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 1 0 0 0 0 0 51 51 48 3 2 1 1 2 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 0 0 0 0 0 0 0 3 0 0 0 117 114 109 5 1 4 1 1 1 1 0 0 0 0 0 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcaactttgcc atgagctgggtccgccaggctccagggaagggactggagtgggtctcgact attagtcctagtggtggtaccaca tattacgcagactccgtgaagggccggttcaccatctccagagacagttccaagaacacgctgtatctgcaaatgaacatcctgagagccgaggacacggccacatattactgt gcgaaaggactatattattatgacagtagtggttatcgggtctttgactac AKGLYYYDSSGYRVFDY IGHV3-23 IGHD3-22 IGHJ4 ggattcacctttagcaactttgcc atgagctgggtccgccaggctccagggaagggactggagtgggtctcgact attagtcctagtggtggtaccaca tattacgcagactccgtgaagggccggttcaccatctccagagacagttccaagaacacgctgtatctgcaaatgaacatcctgagagccgaggacacggccacatattactgt gcgaaaggactatattattatgacagtagtggttatcgggtctttgactac IGA1 13 213 8 5 5 9 3 4 3 0 5 5 JY8QFUQ01BDGDZ 14739 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 1046 97.29 215/221 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD4-11*01 ORF 2 10 7 11 10.7.11 [1.17.38.11] CARLDYTRYFDYW in-frame + tctctggtggctccatcagcagtagtagttactactggggctggatccgccagcccccagggaaggggctggagtggattgggagtatccaatatagtgggagcacctattacaatccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccagttctccctgaagctgacctctgtggccgccgcagacacggctgtgtattactgtgcgagactcgactacactaggtactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [285] t169>c,Y57>Q(- - -)|t171>a,Y57>Q(- - -)|c198>t|c204>t|g275>c,S92>T(+ - +)|a283>g,T95>A(- - -)| t169>c,Y57>Q(- - -)|t171>a,Y57>Q(- - -)| c198>t|c204>t|g275>c,S92>T(+ - +)|a283>g,T95>A(- - -)| a320>t| 319 (320) 228 (229) 222 6 (7) 2 (3) 4 1 0 2 1 0 0 0 (1) 1 1 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 19 2 0 2 0 0 0 1 0 0 0 1 0 0 0 0 117 114 110 4 2 2 1 0 2 0 0 0 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcagtagtagttactac tggggctggatccgccagcccccagggaaggggctggagtggattgggagt atccaatatagtgggagcacc tattacaatccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccagttctccctgaagctgacctctgtggccgccgcagacacggctgtgtattactgt gcgagactcgactacactaggtactttgactac ARLDYTRYFDY IGHV4-39 IGHD4-11 IGHJ4 ggtggctccatcagcagtagtagttactac tggggctggatccgccagcccccagggaaggggctggagtggattgggagt atccaatatagtgggagcacc tattacaatccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccagttctccctgaagctgacctctgtggccgccgcagacacggctgtgtattactgt gcgagactcgactacactaggtactttgactac IGA2 6 216 4 2 2 3 2 3 2 0 2 2 JY8QFUQ01BDIEE 19855 productive Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F or Homsap IGHV4-34*04 F 993 96.26 206/214 nt NA Homsap IGHJ5*02 F 255 100 51/51 nt Homsap IGHD2-15*01 F 2 8 7 21 8.7.21 [1.17.38.11] CARRIQGHCRGGSCSRNNWFDPW in-frame + ctatggtgggtccttcagtggttactactggagttggatccgccagcccccagggaaggggctggagtggattggggaaatcaatcatagtggaagcaccaactacaactcgtccctcaagagtcgagccaccatctcagtagacacgtccaagaagcagttgtccctgaacgtgagctctgtgaccgccgcggacacggctgtgtattactgtgcgagacggattcaaggacattgtaggggtggtagttgctccaggaacaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 79 95 [308, 309] c120>t|c205>t,P69>S(+ - -)|t227>c,V76>A(+ - +)|a234>c|c255>g,N85>K(+ - -)|c261>g,F87>L(+ - -)|g270>c,K90>N(+ - -)|c271>g,L91>V(+ - +)| c120>t| c205>t,P69>S(+ - -)|t227>c,V76>A(+ - +)|a234>c|c255>g,N85>K(+ - -)|c261>g,F87>L(+ - -)|g270>c,K90>N(+ - -)|c271>g,L91>V(+ - +)| g319>c| 318 (320) 220 (222) 212 (213) 8 (9) 2 (3) 6 0 0 2 1 1 0 0 0 1 (2) 3 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 107 7 1 6 0 0 1 1 1 0 0 0 1 3 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctat ggtgggtccttcagtggttactac tggagttggatccgccagcccccagggaaggggctggagtggattggggaa atcaatcatagtggaagcacc aactacaactcgtccctcaagagtcgagccaccatctcagtagacacgtccaagaagcagttgtccctgaacgtgagctctgtgaccgccgcggacacggctgtgtattactgt gcgagacggattcaaggacattgtaggggtggtagttgctccaggaacaactggttcgacccc ARRIQGHCRGGSCSRNNWFDP IGHV4-34 IGHD2-15 IGHJ5 ggtgggtccttcagtggttactac tggagttggatccgccagcccccagggaaggggctggagtggattggggaa atcaatcatagtggaagcacc aactacaactcgtccctcaagagtcgagccaccatctcagtagacacgtccaagaagcagttgtccctgaacgtgagctctgtgaccgccgcggacacggctgtgtattactgt gcgagacggattcaaggacattgtaggggtggtagttgctccaggaacaactggttcgacccc IGA2 8 210 3 5 2 6 1 2 2 0 6 0 JY8QFUQ01BDNIC 18922 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 878 88.99 194/218 nt NA Homsap IGHJ3*01 F 232 96 48/50 nt Homsap IGHD2-2*01 F 2 8 8 18 8.8.18 [1.17.38.11] CARYCSSDSCQGYHDAFDVW in-frame - cttctggatacatcttcacgaaccactggatcggctgggtgcgccagatgccgggtagaggcctggagtggatggggatcatctatcctggtgactccgattccagatacggcccgtccgcccaaggccaggtcaccttctcagtcgacaagtccatcgccaccgcctaccttcagtggagtagcctgaaggcctcggataccgccacgtactactgcgcgagatattgtagtagtgacagctgccaagggtaccatgatgcttttgatgtctggggccaagggacaatggtctccgtctctttagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [303] g74>c|g86>t,S29>I(- - -)|t90>c|c105>g|g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)|c138>g|g141>t|a143>g,K48>R(+ + +)|t189>c|a193>t,T65>S(+ - +)|a202>g,S68>G(+ + -)|t211>g,F71>A(+ - -)|t212>c,F71>A(+ - -)|a232>t,I78>F(+ - -)|c239>t,A80>V(+ - +)|a253>g,S85>A(- + -)|g254>c,S85>A(- + -)|g267>t|c276>t|c294>t|t302>c,M101>T(- - -)|t306>c|t312>c| g74>c| g86>t,S29>I(- - -)|t90>c|c105>g|g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)| c138>g|g141>t|a143>g,K48>R(+ + +)| t189>c|a193>t,T65>S(+ - +)| a202>g,S68>G(+ + -)|t211>g,F71>A(+ - -)|t212>c,F71>A(+ - -)|a232>t,I78>F(+ - -)|c239>t,A80>V(+ - +)|a253>g,S85>A(- + -)|g254>c,S85>A(- + -)|g267>t|c276>t|c294>t|t302>c,M101>T(- - -)|t306>c|t312>c| c319>t| 318 (320) 224 (226) 200 (201) 24 (25) 11 (12) 13 3 1 3 (4) 7 0 0 2 0 2 2 3 1 78 5 4 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 36 24 19 5 2 3 0 1 0 2 0 0 0 0 0 1 1 0 51 51 48 3 2 1 1 0 0 0 0 0 0 0 0 1 1 0 30 24 22 2 1 1 0 0 0 1 0 0 1 0 0 0 0 0 117 114 101 13 5 8 2 0 3 4 0 0 1 0 1 0 1 1 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 (1) 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| cttct ggatacatcttcacgaaccactgg atcggctgggtgcgccagatgccgggtagaggcctggagtggatggggatc atctatcctggtgactccgattcc agatacggcccgtccgcccaaggccaggtcaccttctcagtcgacaagtccatcgccaccgcctaccttcagtggagtagcctgaaggcctcggataccgccacgtactactgc gcgagatattgtagtagtgacagctgccaagggtaccatgatgcttttgatgtc ARYCSSDSCQGYHDAFDV IGHV5-51 IGHD2-2 IGHJ3 ggatacatcttcacgaaccactgg atcggctgggtgcgccagatgccgggtagaggcctggagtggatggggatc atctatcctggtgactccgattcc agatacggcccgtccgcccaaggccaggtcaccttctcagtcgacaagtccatcgccaccgcctaccttcagtggagtagcctgaaggcctcggataccgccacgtactactgc gcgagatattgtagtagtgacagctgccaagggtaccatgatgcttttgatgtc IGA1 23 213 14 9 4 10 10 13 7 3 9 4 JY8QFUQ01BDPXU 10713 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 888 89.55 197/220 nt 88.64 195/220 nt Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD3-10*02 F 2 10 7 11 10.7.11 [1.17.38.11] CVRHVRRFWFDPW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 41 of V-REGION: 'G' (1 nucleotides at position 43 to 43 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 55 of V-REGION: 'A' (1 nucleotides at position 85 to 85 in the user submitted sequence), (cause frameshift); ctctaatggctccatcagcggaagtgtttactactgggcctgGgatccgccagcccccagagaagggtctggagtacattgggaAgcatcttttatagtgggagcacttacttcaatccgtccctcaagagtcgagtcaccctatccgtagacacgtccaggaaccagttctccctgaggctgaagtctgtgaccgccgcagacacggctgtttattattgtgtgagacatgttcgacgattctggttcgacccctggggccagggaaccctggtcaccgtctcttcagcatcccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttctccc NA IGA1 100 95 [285] g79>a,G27>N(- - -)|g80>a,G27>N(- - -)|a100>g,S34>G(+ + -)|t102>a,S34>G(+ + -)|a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|g119>c,G40>A(- + -)|g140>a,G47>E(- - -)|g147>t|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|t165>c|a170>t,Y57>F(- + -)|c195>t|a200>t,Y67>F(- + -)|c204>t|a232>c,I78>L(+ + +)|a251>g,K84>R(+ + +)|a269>g,K90>R(+ + +)|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g303>t|c309>t|c314>t,A105>V(+ - +)| g79>a,G27>N(- - -)|g80>a,G27>N(- - -)|a100>g,S34>G(+ + -)|t102>a,S34>G(+ + -)|a106>g,S36>V(- - -)|g107>t,S36>V(- - -)| g119>c,G40>A(- + -)|g140>a,G47>E(- - -)|g147>t|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|t165>c| a170>t,Y57>F(- + -)|c195>t| a200>t,Y67>F(- + -)|c204>t|a232>c,I78>L(+ + +)|a251>g,K84>R(+ + +)|a269>g,K90>R(+ + +)|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g303>t|c309>t| c314>t,A105>V(+ - +)| 320 228 204 24 6 18 4 5 4 1 1 0 2 1 2 1 3 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 0 6 2 2 0 0 0 0 0 1 0 0 1 0 51 51 45 6 2 4 0 2 0 1 0 0 0 0 2 0 1 0 30 21 19 2 1 1 0 0 1 0 0 0 1 0 0 0 0 0 117 114 105 9 3 6 2 1 2 0 1 0 1 0 0 1 1 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct aatggctccatcagcggaagtgtttactac tgggcctggatccgccagcccccagagaagggtctggagtacattgggagc atcttttatagtgggagcact tacttcaatccgtccctcaagagtcgagtcaccctatccgtagacacgtccaggaaccagttctccctgaggctgaagtctgtgaccgccgcagacacggctgtttattattgt gtgagacatgttcgacgattctggttcgacccc VRHVRRFWFDP IGHV4-39 IGHD3-10 IGHJ5 aatggctccatcagcggaagtgtttactac tgggcctggatccgccagcccccagagaagggtctggagtacattgggagc atcttttatagtgggagcact tacttcaatccgtccctcaagagtcgagtcaccctatccgtagacacgtccaggaaccagttctccctgaggctgaagtctgtgaccgccgcagacacggctgtttattattgt gtgagacatgttcgacgattctggttcgacccc IGA1 23 216 13 10 8 14 5 9 5 1 10 7 JY8QFUQ01BDTL8 18400 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 846 88.02 191/217 nt 87.1 189/217 nt Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD6-19*01 F 1 7 8 17 7.8.17 [2.16.38.11] CARDSASSGYARYDAMDVW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 29 of V-REGION: 1 nucleotides (from position 14 in the user submitted sequence), (cause frameshift)in FR2-IMGT, from codon 49 of V-REGION: 1 nucleotides (from position 61 in the user submitted sequence), (cause frameshift) gcctctggattcagtttagtacacatggcatgaactgggtccgccaggctccagggaagggccggaatgggtctcattcgttaatagtggaagtagttacatctactacgcagactcagtgaggggccgattcaccatctccagagacgacgccaggaattcactgtatctgcaaatgcaccgcctgcgagtcgaggacacggctctctactattgtgcgagagacagtgcctcgtccggctacgctaggtacgacgctatggacgtctggggccaagggaccgcggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [300] c87>g|c90>t|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|t109>c,Y37>H(+ - -)|a112>g,S38>G(+ + -)|t149>c,L50>P(- - -)|g153>a|c164>t,S55>F(- - -)|a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|a195>c|a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|a251>g,K84>R(+ + +)|c255>t|a274>c,N92>H(- - -)|a277>c,S93>R(- - -)|a283>c|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t306>c|c309>t| c87>g|c90>t|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|t109>c,Y37>H(+ - -)|a112>g,S38>G(+ + -)| t149>c,L50>P(- - -)|g153>a|c164>t,S55>F(- - -)| a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|a195>c| a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|a251>g,K84>R(+ + +)|c255>t|a274>c,N92>H(- - -)|a277>c,S93>R(- - -)|a283>c|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t306>c|c309>t| 320 225 199 26 8 18 6 2 5 3 4 1 0 1 3 1 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 17 6 2 4 1 0 1 1 0 1 0 0 1 1 0 0 51 50 47 3 1 2 0 1 1 1 0 0 0 0 0 0 0 0 30 24 19 5 1 4 2 1 0 0 1 0 0 1 0 0 0 0 117 114 102 12 4 8 3 0 3 1 3 0 0 0 2 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcagtttagtacacatggc atgaactgggtccgccaggctccagggaagggccggaatgggtctcattc gttaatagtggaagtagttacatc tactacgcagactcagtgaggggccgattcaccatctccagagacgacgccaggaattcactgtatctgcaaatgcaccgcctgcgagtcgaggacacggctctctactattgt gcgagagacagtgcctcgtccggctacgctaggtacgacgctatggacgtc ARDSASSGYARYDAMDV IGHV3-21 IGHD6-19 IGHJ6 ggattcagtttagtacacatggc atgaactgggtccgccaggctccagggaagggccggaatgggtctcattc gttaatagtggaagtagttacatc tactacgcagactcagtgaggggccgattcaccatctccagagacgacgccaggaattcactgtatctgcaaatgcaccgcctgcgagtcgaggacacggctctctactattgt gcgagagacagtgcctcgtccggctacgctaggtacgacgctatggacgtc IGG2 26 211 16 10 7 12 9 14 5 3 10 8 JY8QFUQ01BE0DI 11723 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 1028 96.38 213/221 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-22*01 F 2 8 8 15 8.8.15 [2.17.38.11] CILLYYDSRGVFSTDYW in-frame - cagcctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggcgtgggtctcacgtattaaaagtgatggcagtggcacaaactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagagcacgctgtttctgcaaatgaacagtctgagagccgaggacacggctgtatattactgtatactgctttactatgatagtagaggtgtcttttcaaccgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] t152>c,V51>A(+ - +)|t171>a,N57>K(+ - -)|g186>c|a190>g,S64>G(+ + -)|g197>a,S66>N(- - -)|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|g303>a| t152>c,V51>A(+ - +)| t171>a,N57>K(+ - -)|g186>c|a190>g,S64>G(+ + -)| g197>a,S66>N(- - -)|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|g303>a| g313>a,A105>I(+ - +)|c314>t,A105>I(+ - +)|a316>c,R106>L(- + -)|g317>t,R106>L(- + -)|a318>g,R106>L(- + -)|g319>c|a320>t| 312 (320) 221 (229) 213 (214) 8 (15) 2 (4) 6 (11) 2 (3) 2 (3) 0 (1) 1 0 (1) 0 1 (2) 1 1 (2) 0 0 (1) 0 78 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 30 24 21 3 1 2 1 0 0 0 0 0 0 1 1 0 0 0 117 114 110 4 1 3 1 2 0 0 0 0 1 0 0 0 0 0 0 (8) 0 0 (1) 0 (7) 0 (2) 0 (5) 0 (1) 0 (1) 0 (1) 0 0 (1) 0 0 (1) 0 0 (1) 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cagcctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggcgtgggtctcacgt attaaaagtgatggcagtggcaca aactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagagcacgctgtttctgcaaatgaacagtctgagagccgaggacacggctgtatattactgt atactgctttactatgatagtagaggtgtcttttcaaccgactac ILLYYDSRGVFSTDY IGHV3-74 IGHD3-22 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggcgtgggtctcacgt attaaaagtgatggcagtggcaca aactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagagcacgctgtttctgcaaatgaacagtctgagagccgaggacacggctgtatattactgt atactgctttactatgatagtagaggtgtcttttcaaccgactac IGA1 8 213 5 3 2 3 3 5 1 1 4 2 JY8QFUQ01BE108 14713 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 809 86.51 186/215 nt NA Homsap IGHJ3*02 F 214 92 46/50 nt Homsap IGHD2-2*01 F 2 8 7 14 8.7.14 [1.17.38.11] CGTSSRAWGADAFDIW in-frame + cctctgggctcagcgtcagtaactaccgcatgggctgggtccgccaggctccagggaaggggctggagtgggtctcagttatttatagagatgatagtacagatcatgtagattccgtgaagggccgattcaccgtttcccgagacaattccaagaacacattgtaccttcagatgaacagtgtgacagccgaggacacggccgtttattattgtggaactagttcccgcgcctggggcgctgatgcctttgatatctggggccaggggacactggtcaccgtctcgtcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [288] t82>c,F28>L(+ - -)|c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|a109>t,N37>Y(- - -)|t112>c,Y38>R(- - -)|a113>g,Y38>R(- - -)|a118>g,S40>G(+ + -)|c174>a,S58>R(- - -)|g176>a,G59>D(- - -)|g188>a,G63>D(- - -)|c192>t|t196>g,Y66>D(- - -)|c198>t,Y66>D(- - -)|t199>c,Y67>H(+ - -)|c201>t,Y67>H(+ - -)|c203>t,A68>V(+ - +)|c207>t|a232>g,I78>V(+ - +)|c234>t,I78>V(+ - +)|a238>c|g258>a|c259>t|t264>c|a270>g|c279>t|c280>g,L94>V(+ - +)|g284>c,R95>T(- - -)|g303>t|c309>t| t82>c,F28>L(+ - -)|c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|a109>t,N37>Y(- - -)|t112>c,Y38>R(- - -)|a113>g,Y38>R(- - -)| a118>g,S40>G(+ + -)| c174>a,S58>R(- - -)|g176>a,G59>D(- - -)|g188>a,G63>D(- - -)|c192>t| t196>g,Y66>D(- - -)|c198>t,Y66>D(- - -)|t199>c,Y67>H(+ - -)|c201>t,Y67>H(+ - -)|c203>t,A68>V(+ - +)|c207>t|a232>g,I78>V(+ - +)|c234>t,I78>V(+ - +)|a238>c|g258>a|c259>t|t264>c|a270>g|c279>t|c280>g,L94>V(+ - +)|g284>c,R95>T(- - -)|g303>t|c309>t| c314>g,A105>G(- + -)|g315>a,A105>G(- + -)|g317>c,R106>T(- - -)|a318>t,R106>T(- - -)|g319>a|a320>g| 313 (320) 216 (223) 187 (188) 29 (35) 10 (12) 19 (23) 4 (5) 4 (6) 9 4 1 1 1 (2) 0 1 (2) 2 (3) 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 1 0 2 0 0 1 0 0 1 0 0 51 51 50 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 30 21 17 4 1 3 0 2 1 0 0 1 0 0 0 0 0 0 117 114 96 18 9 9 2 1 8 2 1 0 0 0 1 1 1 1 1 (8) 1 1 (2) 0 (6) 0 (2) 0 (4) 0 (1) 0 (2) 0 0 0 0 0 (1) 0 0 (1) 0 (1) 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct gggctcagcgtcagtaactaccgc atgggctgggtccgccaggctccagggaaggggctggagtgggtctcagtt atttatagagatgatagtaca gatcatgtagattccgtgaagggccgattcaccgtttcccgagacaattccaagaacacattgtaccttcagatgaacagtgtgacagccgaggacacggccgtttattattgt ggaactagttcccgcgcctggggcgctgatgcctttgatatc GTSSRAWGADAFDI IGHV3-53 IGHD2-2 IGHJ3 gggctcagcgtcagtaactaccgc atgggctgggtccgccaggctccagggaaggggctggagtgggtctcagtt atttatagagatgatagtaca gatcatgtagattccgtgaagggccgattcaccgtttcccgagacaattccaagaacacattgtaccttcagatgaacagtgtgacagccgaggacacggccgtttattattgt ggaactagttcccgcgcctggggcgctgatgcctttgatatc IGA2 29 210 21 8 13 18 8 11 9 1 10 9 JY8QFUQ01BE4D2 2734 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 822 87.38 187/214 nt 86.45 185/214 nt Homsap IGHJ4*02 F (see comment) 213 93.75 45/48 nt Homsap IGHD3-10*01 F 3 8 7 16 8.7.16 [1.17.37.11] CAKESLVRGVISYYFDDW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) in CDR2-IMGT, from codon 58 of V-REGION: 3 nucleotides (from position 87 in the user submitted sequence), (do not cause frameshift)in FR3-IMGT, from codon 76 of V-REGION: 1 nucleotides (from position 131 in the user submitted sequence), (cause frameshift) cctctggattcaactttggcatctataccatggcctgggtccgccaggctccagggaaggggctggagtgggtctcagctattcgtgatcatgatagcacatactacgcagactccgtgcagggccggttttcatctcgagagacaatttcaataatacattgtatctgcaaatggatggcctgcgagccgacgacacggccgtctattactgtgcgaaagagtctctggttcggggagtcatctcatactactttgatgactggggccaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [293] c86>a,T29>N(- + -)|a103>g,S35>G(+ + -)|g107>t,S36>I(- - -)|g112>a,A38>T(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a169>c,S57>R(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g184>c,G62>H(+ - -)|g185>a,G62>H(+ - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|c237>g|c248>t,S83>F(- - -)|g252>t,K84>N(+ - -)|c255>t|g258>a|c259>t|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|a277>g,S93>G(+ + -)|a283>c|g291>c,E97>D(+ - +)|a303>c| c86>a,T29>N(- + -)|a103>g,S35>G(+ + -)|g107>t,S36>I(- - -)|g112>a,A38>T(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)| a169>c,S57>R(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g184>c,G62>H(+ - -)|g185>a,G62>H(+ - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|c237>g|c248>t,S83>F(- - -)|g252>t,K84>N(+ - -)|c255>t|g258>a|c259>t|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|a277>g,S93>G(+ + -)|a283>c|g291>c,E97>D(+ - +)|a303>c| 320 222 195 27 6 21 5 5 5 0 4 1 1 0 3 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 0 0 0 1 0 0 0 0 1 0 51 51 49 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 30 21 15 6 0 6 1 3 0 0 1 0 0 0 1 0 0 0 117 113 98 15 6 9 2 1 5 0 3 0 1 0 1 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaactttggcatctatacc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcagct attcgtgatcatgatagcaca tactacgcagactccgtgcagggccggttttcatctcgagagacaatttcaataatacattgtatctgcaaatggatggcctgcgagccgacgacacggccgtctattactgt gcgaaagagtctctggttcggggagtcatctcatactactttgatgac AKESLVRGVISYYFDD IGHV3-23 IGHD3-10 IGHJ4 ggattcaactttggcatctatacc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcagct attcgtgatcatgatagcaca tactacgcagactccgtgcagggccggttttcatctcgagagacaatttcaataatacattgtatctgcaaatggatggcctgcgagccgacgacacggccgtctattactgt gcgaaagagtctctggttcggggagtcatctcatactactttgatgac IGA1 27 209 15 12 10 17 5 10 6 0 11 10 JY8QFUQ01BE5D4 2484 productive (see comment) Homsap IGHV3-23*05 F 841 87.96 190/216 nt 87.5 189/216 nt Homsap IGHJ2*01 F 229 92.45 49/53 nt Homsap IGHD3-9*01 F 2 8 7 18 8.7.18 [2.17.38.11] CVKVAGDYRTPNYGWHFDLW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 63 of V-REGION: 3 nucleotides (from position 97 in the user submitted sequence), (do not cause frameshift) gcctctggattcacctttggcacctctgacatggcctgggtccgccaggttccaggggaggggctggagtgggtctcacacattgatatcagaggtgccacacagtataaagactccgtgaagggccggttcaccatctccagagacaattccaagaacacactggatctgcaaatgaatagcctgagaggcgaagacacggccgcatattactgtgtgaaagtggccggtgactatagaactcccaactacggctggcacttcgatctctggggccgaggcaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 79 95 [301, 300] a103>g,S35>G(+ + -)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|c113>a,A38>D(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g163>c,A55>H(- - -)|c164>a,A55>H(- - -)|t165>c,A55>H(- - -)|t169>g,Y57>D(- - -)|g173>t,S58>I(- - -)|t177>a,S59>R(- - -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|t196>c,Y66>Q(- - -)|c198>g,Y66>Q(- - -)|g202>a,A68>K(- - -)|c203>a,A68>K(- - -)|g258>a|t262>g,Y88>D(- - -)|c276>t|c287>g,A96>G(- + -)|g291>a|t302>c,V101>A(+ - +)|c314>t,A105>V(+ - +)| a103>g,S35>G(+ + -)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|c113>a,A38>D(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g163>c,A55>H(- - -)|c164>a,A55>H(- - -)|t165>c,A55>H(- - -)| t169>g,Y57>D(- - -)|g173>t,S58>I(- - -)|t177>a,S59>R(- - -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| t196>c,Y66>Q(- - -)|c198>g,Y66>Q(- - -)|g202>a,A68>K(- - -)|c203>a,A68>K(- - -)|g258>a|t262>g,Y88>D(- - -)|c276>t|c287>g,A96>G(- + -)|g291>a|t302>c,V101>A(+ - +)| c314>t,A105>V(+ - +)| 318 222 195 27 3 24 4 3 3 3 1 3 0 1 4 2 1 2 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 0 0 0 1 1 0 0 1 0 0 0 51 51 44 7 0 7 2 0 1 1 0 1 0 0 2 0 0 0 30 21 16 5 0 5 1 0 0 0 0 0 0 1 1 0 1 1 117 114 104 10 3 7 0 3 1 2 0 1 0 0 0 2 0 1 6 6 5 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|agca,172-175(CDR2)|ggta,184-187(CDR2)|agca,190-193(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacctttggcacctctgac atggcctgggtccgccaggttccaggggaggggctggagtgggtctcacac attgatatcagaggtgccaca cagtataaagactccgtgaagggccggttcaccatctccagagacaattccaagaacacactggatctgcaaatgaatagcctgagaggcgaagacacggccgcatattactgt gtgaaagtggccggtgactatagaactcccaactacggctggcacttcgatctc VKVAGDYRTPNYGWHFDL IGHV3-23 IGHD3-9 IGHJ2 ggattcacctttggcacctctgac atggcctgggtccgccaggttccaggggaggggctggagtgggtctcacac attgatatcagaggtgccaca cagtataaagactccgtgaagggccggttcaccatctccagagacaattccaagaacacactggatctgcaaatgaatagcctgagaggcgaagacacggccgcatattactgt gtgaaagtggccggtgactatagaactcccaactacggctggcacttcgatctc IGA2 26 210 12 14 5 15 7 11 3 0 14 9 JY8QFUQ01BE8AK 7663 productive Homsap IGHV3-7*01 F 927 92.17 200/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-22*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARGSYYHYDMFGDYW in-frame + ctctggatttacttttagcaactattggatgacctgggttcgccaggctccagggaaggggctggagtgggtggccaacataaaacaacatggaggtgaaacgtactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcactgcatctgcaaatgaacagcctgagagccgaggacacggctgtctattattgtgcgagagggtcgtattatcattatgatatgttcggcgactactggggccagggagtcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttgccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [291] c84>t|c87>t|t105>c|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|c126>t|g171>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|t262>c,Y88>H(+ - -)|g303>c|c309>t| c84>t|c87>t|t105>c|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|c126>t| g171>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)| a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|t262>c,Y88>H(+ - -)|g303>c|c309>t| a320>g| 319 (320) 224 (225) 207 17 (18) 8 (9) 9 3 (4) 3 4 2 2 0 0 0 3 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 3 1 0 1 2 1 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 0 1 0 0 0 0 0 1 0 0 0 30 24 18 6 2 4 2 2 0 0 1 0 0 0 1 0 0 0 117 114 109 5 2 3 1 0 1 1 1 0 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatttacttttagcaactattgg atgacctgggttcgccaggctccagggaaggggctggagtgggtggccaac ataaaacaacatggaggtgaaacg tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcactgcatctgcaaatgaacagcctgagagccgaggacacggctgtctattattgt gcgagagggtcgtattatcattatgatatgttcggcgactac ARGSYYHYDMFGDY IGHV3-7 IGHD3-22 IGHJ4 ggatttacttttagcaactattgg atgacctgggttcgccaggctccagggaaggggctggagtgggtggccaac ataaaacaacatggaggtgaaacg tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcactgcatctgcaaatgaacagcctgagagccgaggacacggctgtctattattgt gcgagagggtcgtattatcattatgatatgttcggcgactac IGG1 17 213 12 5 7 10 5 7 3 5 4 5 JY8QFUQ01BEABE 8836 productive (see comment) Homsap IGHV3-7*01 F 882 89.86 195/217 nt 89.4 194/217 nt Homsap IGHJ3*02 F 196 88 44/50 nt Homsap IGHD1-1*01 F 3 8 8 10 8.8.10 [1.17.38.11] CARVNKDSTDMW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 52 of V-REGION: 'A' (1 nucleotides at position 69 to 69 in the user submitted sequence), (cause frameshift); ctctggattcacctttagtgactattggatgaggtggttccgccagcctccaggaagggggctggagtAgggtggccagcataaaagaagatggaagtgagaaaggttatgtggactctgtgaagggccgcttcaccatcgccagagacaacgcccagaaatcactgtttttgcagatgaacagcctgagaggcgaggacacggctgtgtatttctgtgcgagagtcaacaaggactctactgatatgtggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 100 100 [279] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c120>g,S40>R(- - -)|g124>t,V42>F(+ - -)|g133>c,A45>P(- - -)|g141>a|a143>g,K48>R(+ + +)|a164>g,N55>S(- - -)|g171>a|c172>g,Q58>E(+ + -)|t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>t,Y66>G(+ - -)|a225>c|t235>g,S79>A(- + -)|a250>c,K84>Q(+ - -)|c255>a,N85>K(+ - -)|a263>t,Y88>F(- + -)|c265>t|a270>g|c287>g,A96>G(- + -)|a308>t,Y103>F(- + -)| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| c120>g,S40>R(- - -)|g124>t,V42>F(+ - -)|g133>c,A45>P(- - -)|g141>a|a143>g,K48>R(+ + +)|a164>g,N55>S(- - -)| g171>a|c172>g,Q58>E(+ + -)| t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>t,Y66>G(+ - -)|a225>c|t235>g,S79>A(- + -)|a250>c,K84>Q(+ - -)|c255>a,N85>K(+ - -)|a263>t,Y88>F(- + -)|c265>t|a270>g|c287>g,A96>G(- + -)|a308>t,Y103>F(- + -)| a320>t| 319 (320) 224 (225) 202 22 (23) 5 (6) 17 5 3 2 0 2 1 2 (3) 0 1 3 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 45 6 1 5 2 1 0 0 0 0 0 0 1 1 1 0 30 24 22 2 1 1 0 1 0 0 0 0 0 0 0 1 0 0 117 114 102 12 3 9 2 0 2 0 2 1 2 0 0 1 0 2 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtgactattgg atgaggtggttccgccagcctccaggaagggggctggagtgggtggccagc ataaaagaagatggaagtgagaaa ggttatgtggactctgtgaagggccgcttcaccatcgccagagacaacgcccagaaatcactgtttttgcagatgaacagcctgagaggcgaggacacggctgtgtatttctgt gcgagagtcaacaaggactctactgatatg ARVNKDSTDM IGHV3-7 IGHD1-1 IGHJ3 ggattcacctttagtgactattgg atgaggtggttccgccagcctccaggaagggggctggagtgggtggccagc ataaaagaagatggaagtgagaaa ggttatgtggactctgtgaagggccgcttcaccatcgccagagacaacgcccagaaatcactgtttttgcagatgaacagcctgagaggcgaggacacggctgtgtatttctgt gcgagagtcaacaaggactctactgatatg IGA2 22 213 10 12 5 11 5 11 4 1 14 3 JY8QFUQ01BEJQP 4406 productive Homsap IGHV4-61*02 F (see comment) 681 79.09 174/220 nt NA Homsap IGHJ4*02 F (see comment) 159 81.25 39/48 nt Homsap IGHD3-9*01 F 2 10 7 16 10.7.16 [1.17.38.11] CARERDRSIGGASELDSW in-frame + Low V-REGION identity (79,09% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ5*01 and Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctcttctgtccccataagcagtggtggttactattggaactggatccggcagcccgccgggaaggggctggagtggatcggacgtgttgacagtaatgggttcgtcaggtacaacagttccctcaagagtcgactttctatgtcggtggacatgtccaagagtcaggtctccctgaggttgaggtctgtgatcgccgcggacacggccgtatactattgtgcgcgagaacgcgataggtcgattggcggagcgtcagaacttgactcctggggccagggatctctggtcatcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [299] g79>t,G27>S(+ + -)|g80>c,G27>S(+ + -)|g83>t,G28>V(- - -)|t85>c,S29>P(+ - -)|c90>a|a106>g,S36>G(+ + -)|c114>t|g119>a,S40>N(- - -)|a147>g|t159>c|g162>a|a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|t169>g,Y57>D(- - -)|t171>c,Y57>D(- - -)|c173>g,T58>S(+ - +)|c174>t,T58>S(+ - +)|g176>a,S59>N(- - -)|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|a197>g,N66>R(+ - -)|c198>g,N66>R(+ - -)|c205>a,P69>S(+ - -)|c206>g,P69>S(+ - -)|c207>t,P69>S(+ - -)|g226>c,V76>L(+ - +)|c228>t,V76>L(+ - +)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|a237>g|a240>g|c245>t,T82>M(- - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|t259>g,F87>V(+ - -)|a269>g,K90>R(+ + +)|c271>t|c276>g,S92>R(- - -)|c284>t,T95>I(- - -)|a291>g|g303>a|t306>c|c309>t|a316>c| g79>t,G27>S(+ + -)|g80>c,G27>S(+ + -)|g83>t,G28>V(- - -)|t85>c,S29>P(+ - -)|c90>a|a106>g,S36>G(+ + -)|c114>t| g119>a,S40>N(- - -)|a147>g|t159>c|g162>a| a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|t169>g,Y57>D(- - -)|t171>c,Y57>D(- - -)|c173>g,T58>S(+ - +)|c174>t,T58>S(+ - +)|g176>a,S59>N(- - -)|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)| a197>g,N66>R(+ - -)|c198>g,N66>R(+ - -)|c205>a,P69>S(+ - -)|c206>g,P69>S(+ - -)|c207>t,P69>S(+ - -)|g226>c,V76>L(+ - +)|c228>t,V76>L(+ - +)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|a237>g|a240>g|c245>t,T82>M(- - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|t259>g,F87>V(+ - -)|a269>g,K90>R(+ + +)|c271>t|c276>g,S92>R(- - -)|c284>t,T95>I(- - -)|a291>g|g303>a|t306>c|c309>t| a316>c| 320 228 181 47 13 34 11 4 12 4 1 2 2 0 2 4 3 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 23 7 2 5 1 0 1 1 0 1 0 0 1 0 2 0 51 51 47 4 3 1 1 2 0 1 0 0 0 0 0 0 0 0 30 21 10 11 0 11 2 1 3 1 0 0 1 0 0 1 1 1 117 114 90 24 7 17 7 1 8 1 0 1 1 0 1 3 0 1 8 8 7 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct tctgtccccataagcagtggtggttactat tggaactggatccggcagcccgccgggaaggggctggagtggatcggacgt gttgacagtaatgggttcgtc aggtacaacagttccctcaagagtcgactttctatgtcggtggacatgtccaagagtcaggtctccctgaggttgaggtctgtgatcgccgcggacacggccgtatactattgt gcgcgagaacgcgataggtcgattggcggagcgtcagaacttgactcc ARERDRSIGGASELDS IGHV4-61 IGHD3-9 IGHJ4 tctgtccccataagcagtggtggttactat tggaactggatccggcagcccgccgggaaggggctggagtggatcggacgt gttgacagtaatgggttcgtc aggtacaacagttccctcaagagtcgactttctatgtcggtggacatgtccaagagtcaggtctccctgaggttgaggtctgtgatcgccgcggacacggccgtatactattgt gcgcgagaacgcgataggtcgattggcggagcgtcagaacttgactcc IGA1 46 216 31 15 16 27 15 19 10 2 18 16 JY8QFUQ01BEQ83 3370 productive Homsap IGHV3-7*01 F 882 89.4 194/217 nt NA Homsap IGHJ1*01 F (see comment) 161 78.85 41/52 nt Homsap IGHD6-19*01 F 1 8 8 10 8.8.10 [1.17.38.11] CVVSSGWLFHYW in-frame + Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctggattcacctctcctagatactggatgaattgggtccgccaggcttccgggaaggggctggagtgggtggccaacataaagcaagacggaagtgaggaaaactttgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaattcaatgtctctacaaatgaacagcctgagagtcgaggacacggctgtatattattgcgttgttagcagtggctggttgttccactactggggccagggctcccgggtcaccgtctcctcagcctccaccaagggcccatcgggtcttccccctggcgccctgctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccggaaccggtgacggtgtcgtggaactcag NA IGG3 79 94 [280, 279] t89>c,F30>S(- - -)|a103>c,S35>P(+ - -)|g104>c,S35>P(+ - -)|c108>a,S36>R(- - -)|t111>c|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|c136>t,P46>S(+ - -)|a138>c,P46>S(+ - -)|t177>c|a193>g,K65>E(+ - -)|t196>a,Y66>N(- - -)|a200>t,Y67>F(- + -)|a225>g|a245>g,N82>S(- - -)|c255>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|c287>t,A96>V(+ - +)|g303>a|c309>t| t89>c,F30>S(- - -)|a103>c,S35>P(+ - -)|g104>c,S35>P(+ - -)|c108>a,S36>R(- - -)|t111>c| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|c136>t,P46>S(+ - -)|a138>c,P46>S(+ - -)| t177>c|a193>g,K65>E(+ - -)| t196>a,Y66>N(- - -)|a200>t,Y67>F(- + -)|a225>g|a245>g,N82>S(- - -)|c255>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|c287>t,A96>V(+ - +)|g303>a|c309>t|t312>c| c314>t,A105>V(+ - +)|g315>t,A105>V(+ - +)|a316>g,R106>V(- - -)|g317>t,R106>V(- - -)|a318>t,R106>V(- - -)|g319>a|a320>g| 311 (320) 216 (225) 194 (195) 22 (30) 7 (10) 15 (20) 3 (5) 3 (4) 5 (6) 3 (4) 3 2 1 (2) 1 1 0 0 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 0 0 2 1 1 0 0 1 0 0 0 51 51 47 4 0 4 0 1 2 0 1 0 0 0 0 0 0 0 30 24 22 2 1 1 1 0 0 1 0 0 0 0 0 0 0 0 116 (117) 114 102 11 5 6 2 2 3 0 1 1 1 1 0 0 0 0 0 (8) 0 0 (1) 0 (7) 0 (2) 0 (5) 0 (2) 0 (1) 0 (1) 0 (0) 0 0 0 (1) 0 0 0 0 (2) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctctcctagatactgg atgaattgggtccgccaggcttccgggaaggggctggagtgggtggccaac ataaagcaagacggaagtgaggaa aactttgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaattcaatgtctctacaaatgaacagcctgagagtcgaggacacggctgtatattattgc gttgttagcagtggctggttgttccactac VVSSGWLFHY IGHV3-7 IGHD6-19 IGHJ1 ggattcacctctcctagatactgg atgaattgggtccgccaggcttccgggaaggggctggagtgggtggccaac ataaagcaagacggaagtgaggaa aactttgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaattcaatgtctctacaaatgaacagcctgagagtcgaggacacggctgtatattattgc gttgttagcagtggctggttgttccactac IGG3 22 213 14 8 8 11 6 11 5 2 10 5 JY8QFUQ01BERKI 5953 productive Homsap IGHV3-72*01 F 921 90.58 202/223 nt NA Homsap IGHJ6*02 F (see comment) 202 80.65 50/62 nt Homsap IGHD2-8*02 F 2 8 10 17 8.10.17 [1.17.38.11] CTRDTTDTGGQSFNGLDVW in-frame + Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) or Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ctctggtttccccttcagtgaccacttcatggactgggtccgccaggctccagggaagggcctggagtgggttggtcgtatcagaaagaaaaccaccggttacaccacagaatatgccgcgtctgttaaaggcagattcatcatttcaagagatgattcagagaactcactgcatctgcaaatgaatggcctgaaaatcgaggacacggccgtgtattactgtactagagatacgacagatacgggtggtcaatccttcaacggtttggacgtctggggccaggggacaacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [305] a81>t|a85>c,T29>P(+ + -)|a113>t,Y38>F(- + -)|g147>c|c162>t|c167>t,T56>I(- - -)|t168>c,T56>I(- - -)|c174>g,N58>K(+ - -)|g178>a,A60>T(- - -)|t180>c,A60>T(- - -)|a182>c,N61>T(- + -)|a184>g,S62>G(+ + -)|c201>t|g213>t|c230>t,T77>I(- - -)|c234>t|a250>g,K84>E(+ - -)|t262>c,Y88>H(+ - -)|c276>t|a277>g,S93>G(+ + -)|c287>t,T96>I(- - -)|g313>a,A105>T(- - -)| a81>t|a85>c,T29>P(+ + -)|a113>t,Y38>F(- + -)| g147>c|c162>t| c167>t,T56>I(- - -)|t168>c,T56>I(- - -)|c174>g,N58>K(+ - -)|g178>a,A60>T(- - -)|t180>c,A60>T(- - -)|a182>c,N61>T(- + -)|a184>g,S62>G(+ + -)| c201>t|g213>t|c230>t,T77>I(- - -)|c234>t|a250>g,K84>E(+ - -)|t262>c,Y88>H(+ - -)|c276>t|a277>g,S93>G(+ + -)|c287>t,T96>I(- - -)| g313>a,A105>T(- - -)| 320 231 209 22 7 15 3 2 7 3 2 0 2 0 1 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 0 0 1 0 2 0 0 0 0 0 51 51 49 2 2 0 0 0 1 0 0 0 0 0 1 0 0 0 30 30 23 7 0 7 1 1 1 2 1 0 0 0 0 1 0 0 117 114 105 9 4 5 2 0 5 1 0 0 0 0 0 0 1 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,165-168(FR2-CDR2)|agct,177-180(CDR2)|aact,253-256(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tgct,312-315(FR3)| ctct ggtttccccttcagtgaccacttc atggactgggtccgccaggctccagggaagggcctggagtgggttggtcgt atcagaaagaaaaccaccggttacaccaca gaatatgccgcgtctgttaaaggcagattcatcatttcaagagatgattcagagaactcactgcatctgcaaatgaatggcctgaaaatcgaggacacggccgtgtattactgt actagagatacgacagatacgggtggtcaatccttcaacggtttggacgtc TRDTTDTGGQSFNGLDV IGHV3-72 IGHD2-8 IGHJ6 ggtttccccttcagtgaccacttc atggactgggtccgccaggctccagggaagggcctggagtgggttggtcgt atcagaaagaaaaccaccggttacaccaca gaatatgccgcgtctgttaaaggcagattcatcatttcaagagatgattcagagaactcactgcatctgcaaatgaatggcctgaaaatcgaggacacggccgtgtattactgt actagagatacgacagatacgggtggtcaatccttcaacggtttggacgtc IGA2 21 219 14 7 8 11 6 10 6 1 5 9 JY8QFUQ01BESQ8 5967 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 958 93.98 203/216 nt 93.52 202/216 nt Homsap IGHJ4*03 F 213 93.75 45/48 nt Homsap IGHD1-26*01 F 1 8 7 11 8.7.11 [2.17.38.11] CAKEGNLGYFDDW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 57 of V-REGION: 3 nucleotides (from position 85 in the user submitted sequence), (do not cause frameshift) gcctctggattcacctttagcagccatcccatgagctgggtccgccaggctccgggaaaagggctggagtggatctcagctttcgttcgtagtggtaacacatactacgtagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaaagaaggtaatctgggctactttgacgactggggtcaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [280, 279] t109>c,Y37>H(+ - -)|g112>c,A38>P(- - -)|a138>g|g141>a|g144>a|g157>a,V53>I(+ - +)|a166>t,I56>F(+ - -)|t168>c,I56>F(+ - -)|g173>t,G58>V(- - -)|a175>c,S59>R(- - -)|g184>a,G62>S(+ + -)|g191>a,S64>N(- - -)|c203>t,A68>V(+ - +)| t109>c,Y37>H(+ - -)|g112>c,A38>P(- - -)| a138>g|g141>a|g144>a|g157>a,V53>I(+ - +)| a166>t,I56>F(+ - -)|t168>c,I56>F(+ - -)|g173>t,G58>V(- - -)|a175>c,S59>R(- - -)|g184>a,G62>S(+ + -)|g191>a,S64>N(- - -)| c203>t,A68>V(+ - +)| 320 224 211 13 3 10 1 5 1 2 1 0 1 0 1 0 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 1 0 0 0 0 1 0 0 0 51 51 47 4 3 1 1 3 0 0 0 0 0 0 0 0 0 0 30 21 15 6 0 6 0 2 0 1 1 0 1 0 0 0 1 0 117 114 113 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacctttagcagccatccc atgagctgggtccgccaggctccgggaaaagggctggagtggatctcagct ttcgttcgtagtggtaacaca tactacgtagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaagaaggtaatctgggctactttgacgac AKEGNLGYFDD IGHV3-23 IGHD1-26 IGHJ4 ggattcacctttagcagccatccc atgagctgggtccgccaggctccgggaaaagggctggagtggatctcagct ttcgttcgtagtggtaacaca tactacgtagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaagaaggtaatctgggctactttgacgac IGA2 13 210 9 4 6 8 3 5 3 0 2 8 JY8QFUQ01BETLC 4513 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 990 95.39 207/217 nt NA Homsap IGHJ5*02 F 237 96.08 49/51 nt Homsap IGHD1-20*01 F 1 8 8 16 8.8.16 [1.17.38.11] CARDPVGISGSVNWFDPW in-frame + ctctggattcaccttcagcaaccataacatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatgtattggtagtagtagtagtgacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaagacacggctgtgtattactgtgcgagagatccggttgggataagtggaagtgtgaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [296] t105>c|g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)|g113>a,S38>N(- - -)|c164>g,S55>C(- - -)|c165>t,S55>C(- - -)|a169>g,S57>G(+ + -)|t190>g,Y64>D(- - -)|c287>t,A96>V(+ - +)|g291>a| t105>c|g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)|g113>a,S38>N(- - -)| c164>g,S55>C(- - -)|c165>t,S55>C(- - -)| a169>g,S57>G(+ + -)|t190>g,Y64>D(- - -)| c287>t,A96>V(+ - +)|g291>a| 320 225 215 10 2 8 1 3 2 2 0 0 0 0 0 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 2 0 2 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 1 0 0 0 0 0 0 1 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 0 1 117 114 112 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagcaaccataac atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatgt attggtagtagtagtagtgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaagacacggctgtgtattactgt gcgagagatccggttgggataagtggaagtgtgaactggttcgacccc ARDPVGISGSVNWFDP IGHV3-21 IGHD1-20 IGHJ5 ggattcaccttcagcaaccataac atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatgt attggtagtagtagtagtgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaagacacggctgtgtattactgt gcgagagatccggttgggataagtggaagtgtgaactggttcgacccc IGA2 10 213 8 2 5 6 3 4 1 1 3 5 JY8QFUQ01BEUNL 21941 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 981 94.93 206/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [1.17.38.11] CTRDIGGRYSYW in-frame - ctctggattcaccttcagtagctactggatgcattgggtccgccaagctccagggaaggggctggagtgggtctcacgtattcatagtgatgggactaccacatactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaggaacacgttgtatctgcaattgaacagtctgagagccgaggacacggctgtgtattattgtacaagagacatcggtgggcgctactcctactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [278] c120>t|t152>a,V51>E(- + -)|a169>c,N57>H(- - -)|g188>c,S63>T(+ - +)|g191>c,S64>T(+ - +)|a196>t,S66>Y(+ - -)|g197>a,S66>Y(+ - -)|a251>g,K84>R(+ + +)|c259>t|a271>t,M91>L(+ + -)|c309>t|g313>a,A105>T(- - -)| c120>t|t152>a,V51>E(- + -)| a169>c,N57>H(- - -)|g188>c,S63>T(+ - +)|g191>c,S64>T(+ - +)| a196>t,S66>Y(+ - -)|g197>a,S66>Y(+ - -)|a251>g,K84>R(+ + +)|c259>t|a271>t,M91>L(+ + -)|c309>t| g313>a,A105>T(- - -)| 320 225 213 12 3 9 1 2 3 0 1 0 2 1 2 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 0 1 0 0 0 0 1 0 0 0 0 30 24 21 3 0 3 0 0 0 0 1 0 0 0 2 0 0 0 117 114 108 6 2 4 1 1 2 0 0 0 2 0 0 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcattgggtccgccaagctccagggaaggggctggagtgggtctcacgt attcatagtgatgggactaccaca tactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaggaacacgttgtatctgcaattgaacagtctgagagccgaggacacggctgtgtattattgt acaagagacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcaccttcagtagctactgg atgcattgggtccgccaagctccagggaaggggctggagtgggtctcacgt attcatagtgatgggactaccaca tactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaggaacacgttgtatctgcaattgaacagtctgagagccgaggacacggctgtgtattattgt acaagagacatcggtgggcgctactcctac IGA2 11 213 5 6 4 6 1 5 3 0 5 3 JY8QFUQ01BF8CL 16921 productive Homsap IGHV4-61*02 F 1037 96.83 214/221 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-22*01 F 2 10 7 16 10.7.16 [1.17.38.11] CARGPKYYYDSSGFEDLW in-frame + tctctggtggctccatcagcagtggtcgttactactggagctggatccggcagcccgccgggaagggactggaatggattgggcgtatttataccagtgggagcaccaactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagctctccctgaagttgagttctgtgaccgccgcagacacggccgtatattactgtgcgagaggtcccaagtattactatgatagcagtggtttcgaagacctctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [301] a106>c,S36>R(- - -)|g153>a|c168>t|t259>c,F87>L(+ - -)|c271>t|c276>t|g303>a| a106>c,S36>R(- - -)| g153>a| c168>t| t259>c,F87>L(+ - -)|c271>t|c276>t|g303>a| a320>g| 319 (320) 228 (229) 221 7 (8) 5 (6) 2 0 (1) 2 3 1 1 0 0 0 0 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 29 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 21 20 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 117 114 110 4 3 1 0 1 2 1 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcagtggtcgttactac tggagctggatccggcagcccgccgggaagggactggaatggattgggcgt atttataccagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagctctccctgaagttgagttctgtgaccgccgcagacacggccgtatattactgt gcgagaggtcccaagtattactatgatagcagtggtttcgaagacctc ARGPKYYYDSSGFEDL IGHV4-61 IGHD3-22 IGHJ4 ggtggctccatcagcagtggtcgttactac tggagctggatccggcagcccgccgggaagggactggaatggattgggcgt atttataccagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagctctccctgaagttgagttctgtgaccgccgcagacacggccgtatattactgt gcgagaggtcccaagtattactatgatagcagtggtttcgaagacctc IGG1 7 216 6 1 5 5 1 2 4 1 1 1 JY8QFUQ01BF9YK 9916 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 1002 96.73 207/214 nt 95.33 204/214 nt Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD6-19*01 F 1 8 7 13 8.7.13 [1.17.38.11] CARTTSSSGWYFDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 39 of V-REGION: 'C' (1 nucleotides at position 29 to 29 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 41 of V-REGION: 'G' (1 nucleotides at position 38 to 38 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 50 to 50 in the user submitted sequence), (cause frameshift); ctctggaggctccattagtaattactacCtggagttgGgatccggcagcCccccagggaagggactggagtggattgggtatatctattacagtgggagcaccaactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaatcagttcttcctgaagctgacctctgtgaccgctgcggacacggccgtgtattactgtgcgaggactacttctagtagtggctggtactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] t81>a|c90>t|g107>a,S36>N(- - -)|c120>t|c255>t|c263>t,S88>F(- - -)|g275>c,S92>T(+ - +)| t81>a|c90>t|g107>a,S36>N(- - -)| c120>t| c255>t|c263>t,S88>F(- - -)|g275>c,S92>T(+ - +)| a318>g|g319>a|a320>c| 317 (320) 219 (222) 212 7 (10) 4 (7) 3 0 (1) 1 (2) 4 0 0 (1) 0 0 1 1 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 1 1 0 0 0 0 1 0 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 111 3 1 2 0 0 2 0 0 0 0 0 1 0 0 0 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggaggctccattagtaattactac tggagttggatccggcagcccccagggaagggactggagtggattgggtat atctattacagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaatcagttcttcctgaagctgacctctgtgaccgctgcggacacggccgtgtattactgt gcgaggactacttctagtagtggctggtactttgactac ARTTSSSGWYFDY IGHV4-59 IGHD6-19 IGHJ4 ggaggctccattagtaattactac tggagttggatccggcagcccccagggaagggactggagtggattgggtat atctattacagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaatcagttcttcctgaagctgacctctgtgaccgctgcggacacggccgtgtattactgt gcgaggactacttctagtagtggctggtactttgactac IGA1 7 210 5 2 5 6 0 1 2 2 2 1 JY8QFUQ01BFB0Q 8365 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 845 88.37 190/215 nt 87.91 189/215 nt Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD6-19*01 F 1 8 7 15 8.7.15 [1.17.38.11] CAKVYTGAWGDPNFDSW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in CDR2-IMGT, from codon 63 of V-REGION: 1 nucleotides (from position 94 in the user submitted sequence), (cause frameshift) tctggattcagctttagcaactttgccatgacttgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtactttagcacccattatgccgactccgtgaagggccgcttcaccatctccagagacaatttcaggagcaccttatatctgcagatgaacaacctgagagccgacgacacggccatatattactgtgcgaaagtgtacaccggtgcctggggggatcctaattttgactcctggggccagggaaccctggtcaccgtctcctcagcattcccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [291] c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|c164>g,A55>G(- + -)|g184>a,G62>T(+ - -)|g185>c,G62>T(+ - -)|g188>t|a195>c|t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|a204>c|g225>c|c248>t,S83>F(- - -)|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|g258>c|c259>t|g261>a|a270>g|g278>a,S93>N(- - -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)| c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|c164>g,A55>G(- + -)| g184>a,G62>T(+ - -)|g185>c,G62>T(+ - -)|g188>t|a195>c| t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|a204>c|g225>c|c248>t,S83>F(- - -)|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|g258>c|c259>t|g261>a|a270>g|g278>a,S93>N(- - -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)| a320>t| 319 (320) 222 (223) 197 25 (26) 9 (10) 16 3 5 5 1 2 0 1 (2) 0 5 2 1 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 1 0 0 1 0 0 51 51 48 3 0 3 0 0 1 0 0 0 0 0 1 1 0 0 30 23 19 4 2 2 0 1 0 0 1 0 0 0 1 0 1 0 117 114 99 15 7 8 3 3 4 1 1 0 0 0 3 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcagctttagcaactttgcc atgacttgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtactttagcacc cattatgccgactccgtgaagggccgcttcaccatctccagagacaatttcaggagcaccttatatctgcagatgaacaacctgagagccgacgacacggccatatattactgt gcgaaagtgtacaccggtgcctggggggatcctaattttgactcc AKVYTGAWGDPNFDS IGHV3-23 IGHD6-19 IGHJ4 ggattcagctttagcaactttgcc atgacttgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtactttagcacc cattatgccgactccgtgaagggccgcttcaccatctccagagacaatttcaggagcaccttatatctgcagatgaacaacctgagagccgacgacacggccatatattactgt gcgaaagtgtacaccggtgcctggggggatcctaattttgactcc IGA1 25 212 14 11 10 18 4 7 7 2 11 5 JY8QFUQ01BFHSP 3005 productive Homsap IGHV4-4*07 F 818 86.98 187/215 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD6-25*01 F 1 8 7 15 8.7.15 [1.17.38.11] CARQRGGGDRQWAFENW in-frame + tctctggtacctccatcagcacttactattggagttggttccggcagcccgccgagaagggactggagtggattgggcgtatctctgtctttgaaaactctaactacaacccctccctcgagagtcgcatcaccatgtcaatggacacgtccaagaaccagttctccctgacggtgaactctgtgaccgccgcggacacggccgtgtatttttgtgcgagacaaagaggcggcggcgaccgacagtgggcctttgagaactggggccagggaacccaaatcaccgtctccgcagcctccaccaagggcccatcggtcttcccccctgggcaccctcctccaaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 86 88 [292, 295] g82>a,G28>T(+ - -)|g83>c,G28>T(+ - -)|t105>c|g107>c,S36>T(+ - +)|c114>t|c120>t|a124>t,I42>F(+ - -)|g140>a,G47>E(- - -)|a170>c,Y57>S(+ - -)|a172>g,T58>V(- - -)|c173>t,T58>V(- - -)|a175>t,S59>F(- - -)|g176>t,S59>F(- - -)|g188>a,G63>E(- - -)|g189>a,G63>E(- - -)|g191>a,S64>N(- - -)|a193>t,T65>S(+ - +)|c195>t,T65>S(+ - +)|a214>g,K72>E(+ - -)|a225>c|g226>a,V76>I(+ - +)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|a269>c,K90>T(- - -)|c271>g,L91>V(+ - +)|g275>a,S92>N(- - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g82>a,G28>T(+ - -)|g83>c,G28>T(+ - -)|t105>c|g107>c,S36>T(+ - +)|c114>t| c120>t|a124>t,I42>F(+ - -)|g140>a,G47>E(- - -)| a170>c,Y57>S(+ - -)|a172>g,T58>V(- - -)|c173>t,T58>V(- - -)|a175>t,S59>F(- - -)|g176>t,S59>F(- - -)|g188>a,G63>E(- - -)|g189>a,G63>E(- - -)|g191>a,S64>N(- - -)|a193>t,T65>S(+ - +)|c195>t,T65>S(+ - +)| a214>g,K72>E(+ - -)|a225>c|g226>a,V76>I(+ - +)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|a269>c,K90>T(- - -)|c271>g,L91>V(+ - +)|g275>a,S92>N(- - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g319>c| 318 (320) 221 (223) 193 (194) 28 (29) 4 (5) 24 3 8 5 1 3 0 4 0 2 (3) 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 1 1 1 0 0 0 0 2 0 0 0 51 51 48 3 1 2 0 1 1 0 0 0 1 0 0 0 0 0 30 21 11 10 0 10 1 3 2 0 1 0 2 0 0 0 1 0 117 114 104 10 1 9 2 3 1 0 2 0 1 0 0 1 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtacctccatcagcacttactat tggagttggttccggcagcccgccgagaagggactggagtggattgggcgt atctctgtctttgaaaactct aactacaacccctccctcgagagtcgcatcaccatgtcaatggacacgtccaagaaccagttctccctgacggtgaactctgtgaccgccgcggacacggccgtgtatttttgt gcgagacaaagaggcggcggcgaccgacagtgggcctttgagaac ARQRGGGDRQWAFEN IGHV4-4 IGHD6-25 IGHJ4 ggtacctccatcagcacttactat tggagttggttccggcagcccgccgagaagggactggagtggattgggcgt atctctgtctttgaaaactct aactacaacccctccctcgagagtcgcatcaccatgtcaatggacacgtccaagaaccagttctccctgacggtgaactctgtgaccgccgcggacacggccgtgtatttttgt gcgagacaaagaggcggcggcgaccgacagtgggcctttgagaac IGG1 28 210 17 11 13 17 4 11 2 2 11 13 JY8QFUQ01BFI4C 19725 productive (see comment) Homsap IGHV3-7*01 F 986 94.95 207/218 nt 94.5 206/218 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD4-17*01 F 3 8 8 11 8.8.11 [2.17.37.11] CARTSSREITDYW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 88 of V-REGION: 1 nucleotides (from position 170 in the user submitted sequence), (cause frameshift) gcctctggattcacctttggtagctattggatggcctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagcaagatggaagtggtacatactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaattcactgttctgcaaatggacagcctgagagtcgaggacacggctctgtattactgtgcgagaacctcgtcgcgggagattacggattactggggccagggaaccctggtcaccgtttcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [282] a103>g,S35>G(+ + -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a191>g,E64>G(- - -)|g192>t,E64>G(- - -)|a194>c,K65>T(- - -)|a232>g,I78>V(+ - +)|c255>t|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)| a103>g,S35>G(+ + -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)| a191>g,E64>G(- - -)|g192>t,E64>G(- - -)|a194>c,K65>T(- - -)| a232>g,I78>V(+ - +)|c255>t|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)| g319>a|a320>c| 318 (320) 224 (226) 213 11 (13) 1 (3) 10 5 0 (1) 2 0 1 (2) 0 0 0 2 0 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 30 24 21 3 0 3 1 0 0 0 1 0 0 0 0 0 1 0 117 113 108 5 1 4 2 0 2 0 0 0 0 0 1 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacctttggtagctattgg atggcctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaagtggtaca tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaattcactgttctgcaaatggacagcctgagagtcgaggacacggctctgtattactgt gcgagaacctcgtcgcgggagattacggattac ARTSSREITDY IGHV3-7 IGHD4-17 IGHJ4 ggattcacctttggtagctattgg atggcctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaagtggtaca tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaattcactgttctgcaaatggacagcctgagagtcgaggacacggctctgtattactgt gcgagaacctcgtcgcgggagattacggattac IGA2 11 212 7 4 2 5 5 6 1 0 6 4 JY8QFUQ01BFROD 17005 productive Homsap IGHV4-39*02 F (see comment) 757 83.11 182/219 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD2-2*02 F 3 10 7 22 10.7.22 [1.17.38.11] CATIVPSSVTALINDQGRFYFDHW in-frame + Low V-REGION identity (83,11% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tccggtgtctccattagtagtgatacttaccactggggctggatccgccagcccccagggaaggaaccggagtggatcggcaagatctctaacagtgggagcaccttctacagtccgtctttccagagtcgagtcactgtatcgatggaagcgcccaagaaccacatctccctgaaactgaggtctgtgaccgccgcagacacggctgtttattattgtgcgacaatagtcccctcttctgtgacagcacttattaacgatcaaggaaggttctactttgaccactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 94 [316, 317] t78>c|g83>t,G28>V(- - -)|c90>t|c93>t|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>c,S36>T(+ - +)|t112>c,Y38>H(+ - -)|g146>a,G49>E(- - -)|g147>a,G49>E(- - -)|t149>c,L50>P(- - -)|t159>c|g162>c|g164>a,S55>K(- - -)|t165>g,S55>K(- - -)|a170>c,Y57>S(+ - -)|t172>a,Y58>N(- - -)|t174>c,Y58>N(- - -)|a197>t,Y66>F(- + -)|a203>g,N68>S(- - -)|c204>t,N68>S(- - -)|c210>t|c211>t,L71>F(+ - -)|a214>c,K72>Q(+ - -)|c231>t|a232>g,I78>V(+ - +)|c237>g|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|c243>a,D81>E(+ - +)|a244>g,T82>A(- - -)|t247>c,S83>P(+ - -)|t259>a,F87>I(+ - -)|g270>a|c276>g,S92>R(- - -)|g303>t|c309>t| t78>c| g83>t,G28>V(- - -)|c90>t|c93>t|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>c,S36>T(+ - +)|t112>c,Y38>H(+ - -)| g146>a,G49>E(- - -)|g147>a,G49>E(- - -)|t149>c,L50>P(- - -)|t159>c|g162>c|g164>a,S55>K(- - -)|t165>g,S55>K(- - -)| a170>c,Y57>S(+ - -)|t172>a,Y58>N(- - -)|t174>c,Y58>N(- - -)| a197>t,Y66>F(- + -)|a203>g,N68>S(- - -)|c204>t,N68>S(- - -)|c210>t|c211>t,L71>F(+ - -)|a214>c,K72>Q(+ - -)|c231>t|a232>g,I78>V(+ - +)|c237>g|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|c243>a,D81>E(+ - +)|a244>g,T82>A(- - -)|t247>c,S83>P(+ - -)|t259>a,F87>I(+ - -)|g270>a|c276>g,S92>R(- - -)|g303>t|c309>t| g317>c,R106>T(- - -)|g319>a|a320>t| 316 (320) 223 (227) 186 (187) 37 (40) 11 (13) 26 (27) 5 6 (7) 7 6 2 1 1 (2) 2 2 (3) 2 2 1 78 3 2 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 30 23 7 2 5 1 1 2 1 0 0 0 0 1 0 1 0 51 51 44 7 2 5 0 3 0 2 0 0 0 0 1 0 0 1 30 21 18 3 0 3 0 0 0 1 1 0 0 1 0 0 0 0 117 114 95 19 6 13 4 2 5 1 1 1 1 1 0 2 1 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 0 (1) 0 0 0 0 0 (1) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tcc ggtgtctccattagtagtgatacttaccac tggggctggatccgccagcccccagggaaggaaccggagtggatcggcaag atctctaacagtgggagcacc ttctacagtccgtctttccagagtcgagtcactgtatcgatggaagcgcccaagaaccacatctccctgaaactgaggtctgtgaccgccgcagacacggctgtttattattgt gcgacaatagtcccctcttctgtgacagcacttattaacgatcaaggaaggttctactttgaccac ATIVPSSVTALINDQGRFYFDH IGHV4-39 IGHD2-2 IGHJ4 ggtgtctccattagtagtgatacttaccac tggggctggatccgccagcccccagggaaggaaccggagtggatcggcaag atctctaacagtgggagcacc ttctacagtccgtctttccagagtcgagtcactgtatcgatggaagcgcccaagaaccacatctccctgaaactgaggtctgtgaccgccgcagacacggctgtttattattgt gcgacaatagtcccctcttctgtgacagcacttattaacgatcaaggaaggttctactttgaccac IGG1 36 216 23 13 13 20 10 16 8 2 18 8 JY8QFUQ01BFWP5 12187 productive Homsap IGHV3-7*01 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-22*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARGSYYYYDTNGDYW in-frame - ctctggatttacctttacaaattattggatgagctgggtccgccaggctccagggagggggctggagtgggtggccagcgtaaaacaagatggaggtgagaaatactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagggtcctactattattatgatactaatggcgactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 100 100 [290] c84>t|g104>c,S35>T(+ - +)|t105>a,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a143>g,K48>R(+ + +)|a164>g,N55>S(- - -)|a166>g,I56>V(+ - +)|g171>a|a187>g,S63>G(+ + -)|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)| c84>t|g104>c,S35>T(+ - +)|t105>a,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a143>g,K48>R(+ + +)|a164>g,N55>S(- - -)| a166>g,I56>V(+ - +)|g171>a|a187>g,S63>G(+ + -)| a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)| a320>g| 319 (320) 224 (225) 212 12 (13) 2 (3) 10 5 (6) 2 2 0 1 0 0 1 1 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 2 0 0 0 0 1 1 0 0 0 51 51 49 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 2 1 0 0 0 0 0 0 0 0 0 0 117 114 112 2 0 2 1 0 0 0 1 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatttacctttacaaattattgg atgagctgggtccgccaggctccagggagggggctggagtgggtggccagc gtaaaacaagatggaggtgagaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggtcctactattattatgatactaatggcgactat ARGSYYYYDTNGDY IGHV3-7 IGHD3-22 IGHJ4 ggatttacctttacaaattattgg atgagctgggtccgccaggctccagggagggggctggagtgggtggccagc gtaaaacaagatggaggtgagaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggtcctactattattatgatactaatggcgactat IGA2 12 213 9 3 4 5 5 7 0 2 4 6 JY8QFUQ01BFXYS 15480 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*02 F or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 836 87.91 189/215 nt 87.44 188/215 nt Homsap IGHJ5*02 F 147 76.47 39/51 nt Homsap IGHD6-13*01 F 1 8 7 16 8.7.16 [1.17.38.11] CAKTQPFGTTWYGATDAW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 57 of V-REGION: 3 nucleotides (from position 84 in the user submitted sequence), (do not cause frameshift) cctctgggtttacctttagcaactttgccatggcttgggtccgccaggctccaagaaaggggctggagtgggtctcagctattgctcgtggcggtgacacacactaccgagactccgtgaagggccgattcaccatctccagagacaattctaagaacacactgattctgcagatgagcagcctaagagccgaggacacggccttatattactgtgcaaagacgcagccgtttggcaccacctggtacggtgctacggatgcgtggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [295] a81>g|c84>t|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c120>t,S40>A(- + -)|g139>a,G47>R(- - -)|g141>a,G47>R(- - -)|g173>c,G58>A(- + -)|a175>c,S59>R(- - -)|t186>c|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|t196>c,Y66>H(+ - -)|g202>c,A68>R(- - -)|c203>g,A68>R(- - -)|g225>a|c249>t|g258>a|t262>a,Y88>I(- - -)|a263>t,Y88>I(- - -)|a270>g|a275>g,N92>S(- - -)|g282>a|g301>t,V101>L(+ - +)|g315>a| a81>g|c84>t|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c120>t,S40>A(- + -)|g139>a,G47>R(- - -)|g141>a,G47>R(- - -)| g173>c,G58>A(- + -)|a175>c,S59>R(- - -)|t186>c|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| t196>c,Y66>H(+ - -)|g202>c,A68>R(- - -)|c203>g,A68>R(- - -)|g225>a|c249>t|g258>a|t262>a,Y88>I(- - -)|a263>t,Y88>I(- - -)|a270>g|a275>g,N92>S(- - -)|g282>a|g301>t,V101>L(+ - +)| g315>a|a318>g|g319>a|a320>c| 317 (320) 220 (223) 193 27 (30) 9 (12) 18 5 (6) 8 (9) 3 2 1 (2) 0 2 1 3 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 1 1 1 0 0 0 1 0 0 0 0 0 51 51 46 5 0 5 1 2 1 0 0 0 0 0 1 0 0 0 30 21 16 5 1 4 1 1 0 1 1 0 0 0 1 0 0 0 117 114 102 12 5 7 2 3 1 1 0 0 1 1 1 1 1 0 5 (8) 5 4 1 (4) 1 (4) 0 0 (1) 1 (2) 0 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct gggtttacctttagcaactttgcc atggcttgggtccgccaggctccaagaaaggggctggagtgggtctcagct attgctcgtggcggtgacaca cactaccgagactccgtgaagggccgattcaccatctccagagacaattctaagaacacactgattctgcagatgagcagcctaagagccgaggacacggccttatattactgt gcaaagacgcagccgtttggcaccacctggtacggtgctacggatgcg AKTQPFGTTWYGATDA IGHV3-23 IGHD6-13 IGHJ5 gggtttacctttagcaactttgcc atggcttgggtccgccaggctccaagaaaggggctggagtgggtctcagct attgctcgtggcggtgacaca cactaccgagactccgtgaagggccgattcaccatctccagagacaattctaagaacacactgattctgcagatgagcagcctaagagccgaggacacggccttatattactgt gcaaagacgcagccgtttggcaccacctggtacggtgctacggatgcg IGG2 26 210 17 9 10 15 7 11 5 3 12 6 JY8QFUQ01BFYFA 15933 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F (see comment) 186 87.5 42/48 nt Homsap IGHD3-9*01 F 2 8 8 19 8.8.19 [1.17.38.11] CAKAYDDVLTSYYIAAFIDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcaccttcagtagctatgccatgcattgggtccgccaggctccaggcaaggggccggagtgggtggcagttatatcatatgatggaagtcataaagactatgcagcctccgtgaagggccgattcaccatctccagagacaattccaagagtacgttgtatctgcaaatgaacagcctgagacctgaggacacggctttatattcctgtgcgaaggcatatgacgatgttttgacttcttattacatagcagcttttattgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [305] g113>c,G38>A(- + -)|c120>t|t149>c,L50>P(- - -)|a190>c,N64>H(- - -)|t196>g,Y66>D(- - -)|a206>c,D69>A(- - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c259>t|g286>c,A96>P(- - -)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|a308>c,Y103>S(+ - -)| g113>c,G38>A(- + -)| c120>t|t149>c,L50>P(- - -)| a190>c,N64>H(- - -)| t196>g,Y66>D(- - -)|a206>c,D69>A(- - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c259>t|g286>c,A96>P(- - -)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|a308>c,Y103>S(+ - -)| g317>a,R106>K(+ + +)|a318>g,R106>K(+ + +)|a320>c| 316 (320) 221 (225) 208 (209) 13 (16) 2 (3) 11 (13) 1 (2) 1 (2) 3 1 3 (4) 0 0 0 2 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 49 2 1 1 0 0 1 1 0 0 0 0 0 0 0 0 30 24 23 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 117 114 105 9 1 8 1 1 2 0 2 0 0 0 1 0 1 1 4 (8) 4 4 (5) 0 (3) 0 (1) 0 (2) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctatgcc atgcattgggtccgccaggctccaggcaaggggccggagtgggtggcagtt atatcatatgatggaagtcataaa gactatgcagcctccgtgaagggccgattcaccatctccagagacaattccaagagtacgttgtatctgcaaatgaacagcctgagacctgaggacacggctttatattcctgt gcgaaggcatatgacgatgttttgacttcttattacatagcagcttttattgactcc AKAYDDVLTSYYIAAFIDS IGHV3-30 IGHD3-9 IGHJ4 ggattcaccttcagtagctatgcc atgcattgggtccgccaggctccaggcaaggggccggagtgggtggcagtt atatcatatgatggaagtcataaa gactatgcagcctccgtgaagggccgattcaccatctccagagacaattccaagagtacgttgtatctgcaaatgaacagcctgagacctgaggacacggctttatattcctgt gcgaaggcatatgacgatgttttgacttcttattacatagcagcttttattgactcc IGA1 13 213 6 7 4 7 2 6 2 0 9 2 JY8QFUQ01BFZOR 18962 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 824 86.7 189/218 nt 85.78 187/218 nt Homsap IGHJ4*02 F 123 72.92 35/48 nt Homsap IGHD6-19*01 F 1 8 8 10 8.8.10 [1.17.38.11] CMNELGYCSGGG in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 41 of V-REGION: 'G' (1 nucleotides at position 38 to 38 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 62 to 62 in the user submitted sequence), (cause frameshift); cctctggattcacttttaggagtcatatgatgagttgGggtccgccagactccagggaaggGggctggaatgggtctcaagtattcgagccagtggtgataggacacactatgcagactccgtgaggggccgcttcaccatctccagagacaactccaagaacacgatgtatttgcaaatgcacagcctgagagtcgacgacacggccgtatactactgtatgaacgaactcggatattgtagtggtggggggggccagggagccctggtctccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [281] c87>t|c105>g,S35>R(- - -)|c108>t|t109>c,Y37>H(+ - -)|g112>a,A38>M(+ - -)|c113>t,A38>M(+ - -)|c114>g,A38>M(+ - -)|c120>t|g133>a,A45>T(- - -)|g153>a|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|a169>c,S57>R(- - -)|t171>a,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g188>a,G63>D(- - -)|c192>g,S64>R(- - -)|t196>c,Y66>H(+ - -)|c201>t|a215>g,K72>R(+ + +)|g225>c|t246>c|c259>a,L87>M(+ + -)|c265>t|a274>c,N92>H(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t306>c|g313>a,A105>M(+ - -)|c314>t,A105>M(+ - -)| c87>t|c105>g,S35>R(- - -)|c108>t|t109>c,Y37>H(+ - -)|g112>a,A38>M(+ - -)|c113>t,A38>M(+ - -)|c114>g,A38>M(+ - -)| c120>t|g133>a,A45>T(- - -)|g153>a|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| a169>c,S57>R(- - -)|t171>a,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g188>a,G63>D(- - -)|c192>g,S64>R(- - -)| t196>c,Y66>H(+ - -)|c201>t|a215>g,K72>R(+ + +)|g225>c|t246>c|c259>a,L87>M(+ + -)|c265>t|a274>c,N92>H(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t306>c| g313>a,A105>M(+ - -)|c314>t,A105>M(+ - -)|a318>c,K106>N(+ - -)| 320 226 194 32 9 23 1 6 8 5 3 1 0 1 3 4 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 2 5 0 1 3 1 0 0 0 0 0 2 0 0 51 51 46 5 2 3 0 3 1 0 0 0 0 0 0 1 0 0 30 24 18 6 0 6 0 1 0 1 1 0 0 1 1 1 0 0 117 114 103 11 5 6 1 0 3 3 1 1 0 0 2 0 0 0 8 8 5 3 0 3 0 1 1 0 1 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacttttaggagtcatatg atgagttgggtccgccagactccagggaaggggctggaatgggtctcaagt attcgagccagtggtgataggaca cactatgcagactccgtgaggggccgcttcaccatctccagagacaactccaagaacacgatgtatttgcaaatgcacagcctgagagtcgacgacacggccgtatactactgt atgaacgaactcggatattgtagtggtggg MNELGYCSGG IGHV3-23 IGHD6-19 IGHJ4 ggattcacttttaggagtcatatg atgagttgggtccgccagactccagggaaggggctggaatgggtctcaagt attcgagccagtggtgataggaca cactatgcagactccgtgaggggccgcttcaccatctccagagacaactccaagaacacgatgtatttgcaaatgcacagcctgagagtcgacgacacggccgtatactactgt atgaacgaactcggatattgtagtggtggg IGA1 29 213 18 11 12 20 6 9 7 2 9 11 JY8QFUQ01BG2F7 20037 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 882 89.86 195/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 2 8 8 17 8.8.17 [1.17.38.11] CARGEFYYETSGRRGFDYW in-frame + ctctggattcaccttcactacctcctggatgcactgggtccgccaagctccagggaaggggctaatgtgggtcgcacgtattaataaggatggcagtagtacaagttatgaggactccgtgaagggccgattcaccatctccagagacaacgccaagaccacactgtacttggaaatggacagtctgagagtcgaggacacggctatgtattattgtgcaagaggggaattttactatgaaactagtggtcgaaggggttttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [299] g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|g150>a|g151>a,V51>M(+ - -)|t160>g,S54>A(- + -)|g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g186>c|c192>t|c198>t|c201>t|c203>a,A68>E(- - -)|a254>c,N85>T(- + -)|g258>a|t264>c|c265>t|c268>g,Q90>E(+ + -)|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g301>a,V101>M(+ - -)|c309>t| g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)| g150>a|g151>a,V51>M(+ - -)|t160>g,S54>A(- + -)| g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g186>c|c192>t| c198>t|c201>t|c203>a,A68>E(- - -)|a254>c,N85>T(- + -)|g258>a|t264>c|c265>t|c268>g,Q90>E(+ + -)|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g301>a,V101>M(+ - -)|c309>t| a320>g| 319 (320) 224 (225) 202 22 (23) 9 (10) 13 1 (2) 5 6 1 2 1 0 0 3 1 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 0 1 0 0 0 2 0 0 0 51 51 48 3 1 2 0 2 0 0 0 0 0 0 0 0 0 1 30 24 20 4 2 2 0 1 1 0 0 0 0 0 1 0 0 1 117 114 102 12 6 6 1 2 5 1 1 1 0 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcactacctcctgg atgcactgggtccgccaagctccagggaaggggctaatgtgggtcgcacgt attaataaggatggcagtagtaca agttatgaggactccgtgaagggccgattcaccatctccagagacaacgccaagaccacactgtacttggaaatggacagtctgagagtcgaggacacggctatgtattattgt gcaagaggggaattttactatgaaactagtggtcgaaggggttttgactac ARGEFYYETSGRRGFDY IGHV3-74 IGHD3-22 IGHJ4 ggattcaccttcactacctcctgg atgcactgggtccgccaagctccagggaaggggctaatgtgggtcgcacgt attaataaggatggcagtagtaca agttatgaggactccgtgaagggccgattcaccatctccagagacaacgccaagaccacactgtacttggaaatggacagtctgagagtcgaggacacggctatgtattattgt gcaagaggggaattttactatgaaactagtggtcgaaggggttttgactac IGA1 22 213 13 9 11 16 2 6 7 2 8 5 JY8QFUQ01BG5T6 11168 productive Homsap IGHV3-7*01 F 990 95.39 207/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSDFYSTGYW in-frame - ctctggattcagctttagaacctattggatgggctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagcacgatggaagtgacaaatactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcactgtttttgcaaatgaacagcctgagagccgaggacacggctgtgtactactgtgcgattagtagtgatttttactcgacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [281] c86>g,T29>S(+ - +)|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|a118>g,S40>G(+ + -)|a174>c,Q58>H(- + -)|g192>c,E64>D(+ - +)|a232>g,I78>V(+ - +)|a263>t,Y88>F(- + -)|c265>t|t306>c| c86>g,T29>S(+ - +)|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)| a118>g,S40>G(+ + -)| a174>c,Q58>H(- + -)|g192>c,E64>D(+ - +)| a232>g,I78>V(+ - +)|a263>t,Y88>F(- + -)|c265>t|t306>c| g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 316 (320) 221 (225) 211 10 (14) 2 (4) 8 (10) 2 (3) 0 (1) 1 1 1 0 1 (2) 1 2 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 0 0 0 0 1 1 1 0 0 51 51 50 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 1 0 0 0 1 0 0 0 117 114 110 4 2 2 1 0 1 1 0 0 1 0 0 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagctttagaacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcacgatggaagtgacaaa tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcactgtttttgcaaatgaacagcctgagagccgaggacacggctgtgtactactgt gcgattagtagtgatttttactcgacgggctac AISSDFYSTGY IGHV3-7 IGHD3-22 IGHJ4 ggattcagctttagaacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcacgatggaagtgacaaa tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcactgtttttgcaaatgaacagcctgagagccgaggacacggctgtgtactactgt gcgattagtagtgatttttactcgacgggctac IGA2 10 213 4 6 1 4 3 6 2 0 3 5 JY8QFUQ01BG66X 7879 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 846 88.02 191/217 nt NA Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD6-19*01 F 1 8 8 15 8.8.15 [1.17.38.11] CAKVYTGAWGDPNFDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcagctttagcaactttgccatgacttgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtacttttagcacccattatgccgactccgtgaagggccgcttcaccatctccagagacaatttcaggagcaccttatatctgcagatgaacaacctgagagccgacgacacggccatatattactgtgcgaaagtgtacaccggtgcctggggggatcctaattttgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [293] c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|c164>g,A55>G(- + -)|g184>a,G62>T(+ - -)|g185>c,G62>T(+ - -)|g187>t,G63>F(- - -)|g188>t,G63>F(- - -)|a195>c|t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|a204>c|g225>c|c248>t,S83>F(- - -)|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|g258>c|c259>t|g261>a|a270>g|g278>a,S93>N(- - -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)| c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|c164>g,A55>G(- + -)| g184>a,G62>T(+ - -)|g185>c,G62>T(+ - -)|g187>t,G63>F(- - -)|g188>t,G63>F(- - -)|a195>c| t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|a204>c|g225>c|c248>t,S83>F(- - -)|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|g258>c|c259>t|g261>a|a270>g|g278>a,S93>N(- - -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)| a320>t| 319 (320) 224 (225) 198 26 (27) 8 (9) 18 3 5 5 1 2 0 1 (2) 0 5 2 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 1 0 0 1 0 0 51 51 48 3 0 3 0 0 1 0 0 0 0 0 1 1 0 0 30 24 19 5 1 4 0 1 0 0 1 0 0 0 1 0 2 0 117 114 99 15 7 8 3 3 4 1 1 0 0 0 3 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagctttagcaactttgcc atgacttgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtacttttagcacc cattatgccgactccgtgaagggccgcttcaccatctccagagacaatttcaggagcaccttatatctgcagatgaacaacctgagagccgacgacacggccatatattactgt gcgaaagtgtacaccggtgcctggggggatcctaattttgactcc AKVYTGAWGDPNFDS IGHV3-23 IGHD6-19 IGHJ4 ggattcagctttagcaactttgcc atgacttgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtacttttagcacc cattatgccgactccgtgaagggccgcttcaccatctccagagacaatttcaggagcaccttatatctgcagatgaacaacctgagagccgacgacacggccatatattactgt gcgaaagtgtacaccggtgcctggggggatcctaattttgactcc IGA1 26 213 14 12 10 19 4 7 7 1 11 7 JY8QFUQ01BG922 16824 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 968 94.04 205/218 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-3*01 F 1 8 8 10 8.8.10 [1.17.38.11] CARGGLEPVDYW in-frame + cctctggattcaccttcagtacatactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcccgtatcaatcctgatgggcgaatcacaaactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggactcggctgtgtactactgtgcaaggggtggtttggagcccgtagattattggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG3 93 100 [280] g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|a162>c|t168>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|g216>t,K72>N(+ - -)|a295>t,T99>S(+ - +)|t306>c| g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)| a162>c| t168>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|g216>t,K72>N(+ - -)|a295>t,T99>S(+ - +)|t306>c| a318>g|a320>g| 317 (320) 223 (226) 210 (211) 13 (15) 3 (5) 10 0 (2) 1 0 2 3 1 1 1 2 0 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 1 0 0 1 0 0 0 51 51 50 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 30 24 18 6 1 5 0 0 0 1 2 0 0 1 1 0 1 0 117 114 110 4 1 3 0 1 0 1 0 0 1 0 0 0 1 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtacatactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcccgt atcaatcctgatgggcgaatcaca aactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggactcggctgtgtactactgt gcaaggggtggtttggagcccgtagattat ARGGLEPVDY IGHV3-74 IGHD3-3 IGHJ4 ggattcaccttcagtacatactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcccgt atcaatcctgatgggcgaatcaca aactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggactcggctgtgtactactgt gcaaggggtggtttggagcccgtagattat IGG3 13 213 3 10 1 6 2 7 2 1 3 7 JY8QFUQ01BGDEX 22247 productive (see comment) Homsap IGHV3-7*01 F (see comment) 743 82.57 180/218 nt 82.11 179/218 nt Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD2-15*01 F 1 8 8 15 8.8.15 [1.17.38.11] CARLKGLTHHDFYFDLW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (82,57% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR3-IMGT at codon 72 of V-REGION: 'G' (1 nucleotides at position 124 to 124 in the user submitted sequence), (cause frameshift); cctctggattcgtctttactaatcattggatgagttgggtccgccaggccacagggaaggggccggagtgggtggccaacatatccccagacggaaatacgaaatattttggggactctgtgaGggggccgattcagcgtctccagagacaacggcaagcagtcatcgtatctggaaatgaataccctgacagtcgatgacacggctgtatacttctgtgcgaggttgaaggggctgacacatcacgacttctacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacacgcgggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaact NA IGG1 93 94 [296] a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>c,Y37>H(+ - -)|c120>t|t135>c|c136>a,P46>T(+ + -)|t149>c,L50>P(- - -)|a169>t,K57>S(- - -)|a170>c,K57>S(- - -)|g171>c,K57>S(- - -)|a173>c,Q58>P(- - -)|t177>c|g188>a,S63>N(- - -)|g190>a,E64>T(- - -)|a191>c,E64>T(- - -)|c198>t|a200>t,Y67>F(- + -)|t203>g,V68>G(- - -)|a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|a232>g,I78>V(+ - +)|c248>g,A83>G(- + -)|a253>c,N85>Q(+ - +)|c255>g,N85>Q(+ - +)|c259>t,L87>S(- - -)|t260>c,L87>S(- - -)|c268>g,Q90>E(+ + -)|c276>t|g278>c,S93>T(+ - +)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)|g303>a|t306>c|a308>t,Y103>F(- + -)| a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>c,Y37>H(+ - -)| c120>t|t135>c|c136>a,P46>T(+ + -)|t149>c,L50>P(- - -)| a169>t,K57>S(- - -)|a170>c,K57>S(- - -)|g171>c,K57>S(- - -)|a173>c,Q58>P(- - -)|t177>c|g188>a,S63>N(- - -)|g190>a,E64>T(- - -)|a191>c,E64>T(- - -)| c198>t|a200>t,Y67>F(- + -)|t203>g,V68>G(- - -)|a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|a232>g,I78>V(+ - +)|c248>g,A83>G(- + -)|a253>c,N85>Q(+ - +)|c255>g,N85>Q(+ - +)|c259>t,L87>S(- - -)|t260>c,L87>S(- - -)|c268>g,Q90>E(+ + -)|c276>t|g278>c,S93>T(+ - +)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)|g303>a|t306>c|a308>t,Y103>F(- + -)| a318>g|g319>t|a320>t| 317 (320) 223 (226) 185 38 (41) 7 (10) 31 3 (4) 4 7 6 4 1 3 (4) 0 4 4 1 (2) 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 1 2 1 0 0 0 0 1 0 0 0 51 51 47 4 2 2 0 0 1 2 0 1 0 0 0 0 0 0 30 24 16 8 1 7 0 2 0 1 3 0 1 0 1 0 0 0 117 114 94 20 4 16 2 1 4 2 1 0 2 0 2 4 1 1 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcgtctttactaatcattgg atgagttgggtccgccaggccacagggaaggggccggagtgggtggccaac atatccccagacggaaatacgaaa tattttggggactctgtgaggggccgattcagcgtctccagagacaacggcaagcagtcatcgtatctggaaatgaataccctgacagtcgatgacacggctgtatacttctgt gcgaggttgaaggggctgacacatcacgacttctacttcgatctc ARLKGLTHHDFYFDL IGHV3-7 IGHD2-15 IGHJ2 ggattcgtctttactaatcattgg atgagttgggtccgccaggccacagggaaggggccggagtgggtggccaac atatccccagacggaaatacgaaa tattttggggactctgtgaggggccgattcagcgtctccagagacaacggcaagcagtcatcgtatctggaaatgaataccctgacagtcgatgacacggctgtatacttctgt gcgaggttgaaggggctgacacatcacgacttctacttcgatctc IGG1 38 213 20 18 11 21 9 17 6 1 18 13 JY8QFUQ01BGEYK 19005 productive (see comment) Homsap IGHV3-11*01 F 901 90.41 198/219 nt 89.95 197/219 nt Homsap IGHJ6*02 F 263 94.83 55/58 nt Homsap IGHD1-26*01 F 3 8 8 11 8.8.11 [2.17.38.11] CVRDYYYYGMDVW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 63 of V-REGION: 'GGG' (3 nucleotides at position 97 to 99 in the user submitted sequence), (do not cause frameshift); gcttctgggttctccttcagcgactacttcatgagttgggtccgccaggctccagggaagggactggagtgggttgcatacattagtagtagtggtGGGactactaaatactacgcagactctgtgaagggccgattcaccatctccagggacaacggcaagaattcattgtttctgcaaatggacagcctgagagtcgacgacacggccatgtatttctgtgtgagagactactattattacgggatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [286] c75>t|a81>g|a85>t,T29>S(+ - +)|t105>c|a113>t,Y38>F(- + -)|c120>t|a124>g,I42>V(+ - +)|g147>a|t160>g,S54>A(- + -)|g188>c,S63>T(+ - +)|c192>t|t194>a,I65>K(- + -)|c248>g,A83>G(- + -)|c255>t|c259>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>M(+ - -)|a308>t,Y103>F(- + -)|c314>t,A105>V(+ - +)| c75>t| a81>g|a85>t,T29>S(+ - +)|t105>c|a113>t,Y38>F(- + -)| c120>t|a124>g,I42>V(+ - +)|g147>a|t160>g,S54>A(- + -)| g188>c,S63>T(+ - +)|c192>t|t194>a,I65>K(- + -)| c248>g,A83>G(- + -)|c255>t|c259>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>M(+ - -)|a308>t,Y103>F(- + -)| c314>t,A105>V(+ - +)| 320 227 205 22 8 14 3 2 7 1 0 0 4 1 2 1 0 1 78 6 5 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 1 0 0 1 0 0 2 0 0 0 0 0 51 51 47 4 2 2 1 1 1 0 0 0 0 0 0 0 0 1 30 24 21 3 1 2 0 0 1 0 0 0 0 1 1 0 0 0 117 114 105 9 2 7 1 1 3 0 0 0 2 0 1 1 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcttct gggttctccttcagcgactacttc atgagttgggtccgccaggctccagggaagggactggagtgggttgcatac attagtagtagtggtactactaaa tactacgcagactctgtgaagggccgattcaccatctccagggacaacggcaagaattcattgtttctgcaaatggacagcctgagagtcgacgacacggccatgtatttctgt gtgagagactactattattacgggatggacgtc VRDYYYYGMDV IGHV3-11 IGHD1-26 IGHJ6 gggttctccttcagcgactacttc atgagttgggtccgccaggctccagggaagggactggagtgggttgcatac attagtagtagtggtactactaaa tactacgcagactctgtgaagggccgattcaccatctccagggacaacggcaagaattcattgtttctgcaaatggacagcctgagagtcgacgacacggccatgtatttctgt gtgagagactactattattacgggatggacgtc IGA1 20 213 11 9 7 10 4 10 4 3 9 4 JY8QFUQ01BGFCA 17763 productive (see comment) Homsap IGHV3-7*01 F 885 90.65 194/214 nt 90.19 193/214 nt Homsap IGHJ5*02 F (see comment) 192 86.27 44/51 nt Homsap IGHD4-23*01 ORF 3 8 8 12 8.8.12 [0.17.38.11] CARGTSVDPGIDHW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 48 of V-REGION: 'GG' (2 nucleotides at position 55 to 56 in the user submitted sequence), (cause frameshift); tggattcacctttagtaattattggatgcactgggtccgccaggtttcaggggaGGggggctggagtgcgtggccaccataaacgaagatggaagtgagagacactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactctctgtttttgcagatgaacagcctgagagccgaggacacggctgtctactactgtgcgagagggacctcagtggacccggggattgaccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [283] g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)|c134>t,A45>V(+ - +)|c136>t,P46>S(+ - -)|a142>g,K48>E(+ - -)|g156>c,W52>C(+ - -)|a164>c,N55>T(- + -)|g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)|t196>c,Y66>H(+ - -)|a232>g,I78>V(+ - +)|a258>t|a263>t,Y88>F(- + -)|c265>t|a270>g|g303>c|t306>c| g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)|c134>t,A45>V(+ - +)|c136>t,P46>S(+ - -)|a142>g,K48>E(+ - -)|g156>c,W52>C(+ - -)|a164>c,N55>T(- + -)| g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)| t196>c,Y66>H(+ - -)|a232>g,I78>V(+ - +)|a258>t|a263>t,Y88>F(- + -)|c265>t|a270>g|g303>c|t306>c| a320>g| 319 (320) 221 (222) 201 20 (21) 5 (6) 15 4 (5) 2 4 2 2 0 2 0 3 1 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 51 51 44 7 0 7 1 1 2 0 2 0 0 0 1 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 1 1 0 0 117 114 106 8 5 3 2 0 1 2 0 0 2 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t ggattcacctttagtaattattgg atgcactgggtccgccaggtttcaggggaggggctggagtgcgtggccacc ataaacgaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactctctgtttttgcagatgaacagcctgagagccgaggacacggctgtctactactgt gcgagagggacctcagtggacccggggattgaccac ARGTSVDPGIDH IGHV3-7 IGHD4-23 IGHJ5 ggattcacctttagtaattattgg atgcactgggtccgccaggtttcaggggaggggctggagtgcgtggccacc ataaacgaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactctctgtttttgcagatgaacagcctgagagccgaggacacggctgtctactactgt gcgagagggacctcagtggacccggggattgaccac IGA1 20 213 12 8 6 10 6 10 5 0 10 5 JY8QFUQ01BGGO2 10248 productive (see comment) Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 873 89.4 194/217 nt 88.48 192/217 nt Homsap IGHJ6*02 F 247 88.71 55/62 nt Homsap IGHD1-26*01 F 3 8 8 21 8.8.21 [1.17.38.11] CVKDLGSYGSPWRAYYYYGMDAW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 11 to 11 in the user submitted sequence), (cause frameshift);in CDR1-IMGT at codon 37 of V-REGION: 'C' (1 nucleotides at position 24 to 24 in the user submitted sequence), (cause frameshift); ctctggattcGaccctcaacagcCaatggcatgcactgggtccgccagactccagacaaggggctggagtgggtggcagtgatttcatatgatggaaatgataaatactatggagatgcagtgacgggccgattcaccatctccagagacacttccaagaacacagtatatctggagatgtacagcctgagacctgaggacacggctgtgtattactgtgtgaaagatcttgggagttacggtagtccttggcgcgcctactattactacggcatggacgcctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [313, 312] t88>c,F30>L(+ - -)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|t109>a,Y37>N(- - -)|g133>a,A45>T(- - -)|g140>a,G47>D(- - -)|t165>g|a168>t|g188>a,S63>N(- - -)|a190>g,N64>D(+ + -)|c203>g,A68>G(- + -)|c207>t|t208>g,S70>A(- + -)|c210>a,S70>A(- + -)|a215>c,K72>T(- - -)|a245>c,N82>T(- + -)|g258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c268>g,Q90>E(+ + -)|a270>g,Q90>E(+ + -)|a274>t,N92>Y(- - -)|g286>c,A96>P(- - -)|c314>t,A105>V(+ - +)|g317>a,R106>K(+ + +)| t88>c,F30>L(+ - -)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|t109>a,Y37>N(- - -)| g133>a,A45>T(- - -)|g140>a,G47>D(- - -)|t165>g| a168>t|g188>a,S63>N(- - -)|a190>g,N64>D(+ + -)| c203>g,A68>G(- + -)|c207>t|t208>g,S70>A(- + -)|c210>a,S70>A(- + -)|a215>c,K72>T(- - -)|a245>c,N82>T(- + -)|g258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c268>g,Q90>E(+ + -)|a270>g,Q90>E(+ + -)|a274>t,N92>Y(- - -)|g286>c,A96>P(- - -)| c314>t,A105>V(+ - +)|g317>a,R106>K(+ + +)| 320 225 200 25 4 21 2 7 2 2 2 1 2 1 1 3 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 0 2 0 0 0 1 0 0 0 0 51 51 48 3 1 2 0 2 0 0 0 0 0 0 0 0 0 1 30 24 21 3 1 2 1 1 0 0 0 0 1 0 0 0 0 0 117 114 101 13 2 11 1 2 1 0 2 1 1 0 1 3 0 1 8 8 6 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccctcaacagcaatggc atgcactgggtccgccagactccagacaaggggctggagtgggtggcagtg atttcatatgatggaaatgataaa tactatggagatgcagtgacgggccgattcaccatctccagagacacttccaagaacacagtatatctggagatgtacagcctgagacctgaggacacggctgtgtattactgt gtgaaagatcttgggagttacggtagtccttggcgcgcctactattactacggcatggacgcc VKDLGSYGSPWRAYYYYGMDA IGHV3-30 IGHD1-26 IGHJ6 ggattcaccctcaacagcaatggc atgcactgggtccgccagactccagacaaggggctggagtgggtggcagtg atttcatatgatggaaatgataaa tactatggagatgcagtgacgggccgattcaccatctccagagacacttccaagaacacagtatatctggagatgtacagcctgagacctgaggacacggctgtgtattactgt gtgaaagatcttgggagttacggtagtccttggcgcgcctactattactacggcatggacgcc IGA2 23 213 11 12 7 12 4 11 3 1 13 6 JY8QFUQ01BGI0O 21809 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 909 91.24 198/217 nt NA Homsap IGHJ6*02 F (see comment) 256 90.32 56/62 nt Homsap IGHD2-2*01 F 3 8 8 20 8.8.20 [1.17.38.11] CAKTVVPAAPNRFYYYYGLDVW in-frame - Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) ctcaggattcacgtttagcaggtatgccatgaactgggtccgccaggctccagagaaggggctggagtgggtctccgctgttagtgatactggtggtacaacagagtacgccgactccgtgaaggggcggttcaccgtctccagagacaattccaagaatacggtgtatctgcaaatgaacagcctgagagtcgaggacacggccgtatattactgtgcgaagaccgtagtgccagctgctcctaatcgattttactactactacggtttggacgtctggggccaggggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [310, 309] t78>a|c87>g|c108>g,S36>R(- - -)|g119>a,S40>N(- - -)|g140>a,G47>E(- - -)|a162>c|a166>g,I56>V(+ - +)|g173>a,G58>D(- - -)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)|t196>g,Y66>E(- - -)|c198>g,Y66>E(- - -)|a204>c|c222>g|a232>g,I78>V(+ - +)|c255>t|c259>g,L87>V(+ - +)|c287>t,A96>V(+ - +)| t78>a| c87>g|c108>g,S36>R(- - -)| g119>a,S40>N(- - -)|g140>a,G47>E(- - -)|a162>c| a166>g,I56>V(+ - +)|g173>a,G58>D(- - -)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)| t196>g,Y66>E(- - -)|c198>g,Y66>E(- - -)|a204>c|c222>g|a232>g,I78>V(+ - +)|c255>t|c259>g,L87>V(+ - +)|c287>t,A96>V(+ - +)| a318>g|g319>a|a320>c| 317 (320) 222 (225) 203 19 (22) 6 (9) 13 2 (3) 3 (4) 2 0 2 (3) 1 0 1 2 5 0 1 78 4 3 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 22 2 1 1 0 0 0 0 0 0 0 0 0 2 0 0 51 51 48 3 1 2 0 2 0 0 1 0 0 0 0 0 0 0 30 24 19 5 0 5 1 1 0 0 0 1 0 0 2 0 0 0 117 114 106 8 3 5 1 0 2 0 1 0 0 0 0 3 0 1 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctca ggattcacgtttagcaggtatgcc atgaactgggtccgccaggctccagagaaggggctggagtgggtctccgct gttagtgatactggtggtacaaca gagtacgccgactccgtgaaggggcggttcaccgtctccagagacaattccaagaatacggtgtatctgcaaatgaacagcctgagagtcgaggacacggccgtatattactgt gcgaagaccgtagtgccagctgctcctaatcgattttactactactacggtttggacgtc AKTVVPAAPNRFYYYYGLDV IGHV3-23 IGHD2-2 IGHJ6 ggattcacgtttagcaggtatgcc atgaactgggtccgccaggctccagagaaggggctggagtgggtctccgct gttagtgatactggtggtacaaca gagtacgccgactccgtgaaggggcggttcaccgtctccagagacaattccaagaatacggtgtatctgcaaatgaacagcctgagagtcgaggacacggccgtatattactgt gcgaagaccgtagtgccagctgctcctaatcgattttactactactacggtttggacgtc IGG1 18 213 7 11 5 13 2 5 4 1 7 6 JY8QFUQ01BGMBI 7768 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23*05 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 810 86.18 187/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD1-20*01 F 1 8 8 14 8.8.14 [1.17.38.11] CARGLTTMTGNGLEHW in-frame + ctctggattcacccttcgcagatatggcatggcgtgggtccgccaggctccggggaaggggctggagtgggtctcatcttctaacagtagtgatgaatccacatactatgcagactccgtgaagggccgcttcaccatttccagagaccattccaagaacacggtgtttttgcaaatgtacagcctgagagccgaagacacggccctctattactgtgcgagagggctaacaacaatgacagggaatggtcttgagcactggggccagggagccctggtcaccgtctcctcaccatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 100 100 [290] t88>c,F30>L(+ - -)|a103>c,S35>R(- - -)|c108>a,S36>R(- - -)|c113>g,A38>G(- + -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c120>g,S40>A(- + -)|a138>g|g163>t,A55>S(- + -)|a166>t,I56>S(- - -)|t167>c,I56>S(- - -)|g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|g172>a,G58>S(+ + -)|g185>a,G62>D(- - -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|a190>t|g191>c|c201>t|g225>c|c234>t|a244>c,N82>H(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c265>t|a274>t,N92>Y(- - -)|g291>a|g301>c,V101>L(+ - +)|a303>c,V101>L(+ - +)|a317>g,K106>R(+ + +)| t88>c,F30>L(+ - -)|a103>c,S35>R(- - -)|c108>a,S36>R(- - -)|c113>g,A38>G(- + -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c120>g,S40>A(- + -)|a138>g|g163>t,A55>S(- + -)| a166>t,I56>S(- - -)|t167>c,I56>S(- - -)|g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|g172>a,G58>S(+ + -)|g185>a,G62>D(- - -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|a190>t|g191>c| c201>t|g225>c|c234>t|a244>c,N82>H(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c265>t|a274>t,N92>Y(- - -)|g291>a|g301>c,V101>L(+ - +)|a303>c,V101>L(+ - +)| a317>g,K106>R(+ + +)|a320>g| 319 (320) 224 (225) 193 31 (32) 8 (9) 23 3 (4) 5 3 3 3 1 4 1 4 3 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 1 1 1 0 0 0 1 0 0 51 51 46 5 1 4 2 0 0 0 0 0 0 0 1 1 1 0 30 24 14 10 2 8 0 4 0 2 0 0 2 1 1 0 0 0 117 114 103 11 5 6 0 1 3 0 2 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 1 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacccttcgcagatatggc atggcgtgggtccgccaggctccggggaaggggctggagtgggtctcatct tctaacagtagtgatgaatccaca tactatgcagactccgtgaagggccgcttcaccatttccagagaccattccaagaacacggtgtttttgcaaatgtacagcctgagagccgaagacacggccctctattactgt gcgagagggctaacaacaatgacagggaatggtcttgagcac ARGLTTMTGNGLEH IGHV3-23 IGHD1-20 IGHJ4 ggattcacccttcgcagatatggc atggcgtgggtccgccaggctccggggaaggggctggagtgggtctcatct tctaacagtagtgatgaatccaca tactatgcagactccgtgaagggccgcttcaccatttccagagaccattccaagaacacggtgtttttgcaaatgtacagcctgagagccgaagacacggccctctattactgt gcgagagggctaacaacaatgacagggaatggtcttgagcac IGA2 30 213 13 17 8 17 5 13 6 2 10 12 JY8QFUQ01BGNSE 18485 productive (see comment) Homsap IGHV4-55*03 P (see comment) 613 75.69 165/218 nt NA Homsap IGHJ4*02 F (see comment) 141 77.08 37/48 nt Homsap IGHD3-16*01 F 3 10 7 16 10.7.16 [2.17.38.11] CARESRNSFGGPTGLDSW in-frame + however the closest V is a pseudogene The submitted sequence and the closest germline V-GENE allele show different CDR1-IMGT amino acid lengths (10 AA in JY8QFUQ01BGNSE ; 9 AA in Homsap_IGHV4-55*03), and low V-REGION identity (75,69% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) gtctctgttgacgccataagcgacctcggttatttctgggcctgggtccgccagcccgccgcgaagggactggagtggatcggacatgcccttggtgatggatataccgaatacaaccccgccctagagagtcgaatcaccgtgtcagtggacaagtccaagaaccagttttccctgacgttggagtccgtgaccgccgcagacacggccacttatttctgtgcgagggaaagtcggaattcatttggcgggccgacaggccttgattcctggggccagggaattgtggtcatcgtctcctcagcatccccggaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtgtcatcgcctgcctggtccagggcttct NA IGA1 93 84 [302] g80>t,G27>V(- - -)|t85>g,S29>A(- + -)|c90>a|a103>c,S35>L(- - -)|g104>t,S35>L(- - -)|t105>c,S35>L(- - -)|a109>t,N37>Y(- - -)|c111>t,N37>Y(- - -)|g113>t,W38>F(+ + -)|g114>c,W38>F(+ + -)|a117>g,*39>W(- - -)|a118>g,I40>A(+ - +)|t119>c,I40>A(+ - +)|c136>g,P46>A(- - -)|a138>c,P46>A(- - -)|g140>c,G47>A(- + -)|g147>a|t159>c|g162>a|g163>c,E55>H(- + -)|a165>t,E55>H(- + -)|a166>g,I56>A(+ - +)|t167>c,I56>A(+ - +)|a170>t,H57>L(- - -)|c172>g,H58>G(+ - -)|a173>g,H58>G(+ - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g189>a|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|c192>t,S64>Y(+ - -)|t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|g207>c|t208>g,S70>A(- + -)|c213>a|a214>g,K72>E(+ - -)|a232>g,M78>V(+ - -)|a240>g|c245>a,T82>K(- - -)|c261>t|a269>c,K90>T(- - -)|c271>t|a274>g,S92>E(- - -)|g275>a,S92>E(- - -)|c276>g,S92>E(- - -)|t279>c|g291>a|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|a308>t,Y103>F(- + -)| g80>t,G27>V(- - -)|t85>g,S29>A(- + -)|c90>a|a103>c,S35>L(- - -)|g104>t,S35>L(- - -)|t105>c,S35>L(- - -)|a109>t,N37>Y(- - -)|c111>t,N37>Y(- - -)|g113>t,W38>F(+ + -)|g114>c,W38>F(+ + -)| a117>g,*39>W(- - -)|a118>g,I40>A(+ - +)|t119>c,I40>A(+ - +)|c136>g,P46>A(- - -)|a138>c,P46>A(- - -)|g140>c,G47>A(- + -)|g147>a|t159>c|g162>a|g163>c,E55>H(- + -)|a165>t,E55>H(- + -)| a166>g,I56>A(+ - +)|t167>c,I56>A(+ - +)|a170>t,H57>L(- - -)|c172>g,H58>G(+ - -)|a173>g,H58>G(+ - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g189>a|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|c192>t,S64>Y(+ - -)| t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|g207>c|t208>g,S70>A(- + -)|c213>a|a214>g,K72>E(+ - -)|a232>g,M78>V(+ - -)|a240>g|c245>a,T82>K(- - -)|c261>t|a269>c,K90>T(- - -)|c271>t|a274>g,S92>E(- - -)|g275>a,S92>E(- - -)|c276>g,S92>E(- - -)|t279>c|g291>a|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|a308>t,Y103>F(- + -)| 311 221 168 53 12 41 9 8 4 6 3 4 5 0 4 3 4 3 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 20 10 1 9 0 0 1 1 1 1 1 0 1 0 3 1 51 51 40 11 3 8 2 2 0 2 1 0 1 0 2 1 0 0 30 21 10 11 1 10 3 3 1 1 0 0 2 0 0 1 0 0 116 114 92 21 7 14 4 3 2 2 1 3 1 0 1 1 1 2 0 aa,40-41(FR1)|ta,108-109(CDR1)|aa,117-118(FR2)|aa,142-143(FR2)|aa,164-165(FR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,108-109(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggta,106-109(CDR1)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct gttgacgccataagcgacctcggttatttc tgggcctgggtccgccagcccgccgcgaagggactggagtggatcggacat gcccttggtgatggatatacc gaatacaaccccgccctagagagtcgaatcaccgtgtcagtggacaagtccaagaaccagttttccctgacgttggagtccgtgaccgccgcagacacggccacttatttctgt gcgagggaaagtcggaattcatttggcgggccgacaggccttgattcc ARESRNSFGGPTGLDS IGHV4-55 IGHD3-16 IGHJ4 gttgacgccataagcgacctcggttatttc tgggcctgggtccgccagcccgccgcgaagggactggagtggatcggacat gcccttggtgatggatatacc gaatacaaccccgccctagagagtcgaatcaccgtgtcagtggacaagtccaagaaccagttttccctgacgttggagtccgtgaccgccgcagacacggccacttatttctgt gcgagggaaagtcggaattcatttggcgggccgacaggccttgattcc IGA1 53 216 27 26 12 27 15 26 10 2 22 19 JY8QFUQ01BGR80 15056 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 1003 96.3 208/216 nt 95.83 207/216 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-8*01 F 2 8 7 8 8.7.8 [1.17.38.11] CARDNYGDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 63 of V-REGION: 1 nucleotides (from position 95 in the user submitted sequence), (cause frameshift) ctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtgttaatggtgatggggtagcacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcaccgtctccccagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [271] a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| 320 224 216 8 4 4 3 1 0 0 0 0 0 0 3 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 23 21 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 4 2 1 1 0 0 0 0 0 0 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac IGA1 8 212 4 4 1 5 3 3 4 0 2 2 JY8QFUQ01BGUIW 19452 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 977 94.5 206/218 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-8*01 F 2 8 8 8 8.8.8 [1.17.38.11] CARDNYGDYW in-frame + cctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgtgttaatggtgatgggggtaggacaacctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcacagtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [273] g153>t|a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)|g197>c,S66>T(+ - +)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g153>t| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)| g197>c,S66>T(+ - +)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 226 214 12 5 7 3 2 0 0 0 0 0 1 3 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 30 24 20 4 0 4 3 0 0 0 0 0 0 0 0 1 0 0 117 114 107 7 4 3 0 2 0 0 0 0 0 1 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgt gttaatggtgatgggggtaggaca acctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgt gttaatggtgatgggggtaggaca acctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac IGA2 12 213 5 7 2 8 3 4 5 0 3 4 JY8QFUQ01BGVCA 11554 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 936 92.63 201/217 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [1.17.38.11] CTRDIGGRYSYW in-frame + ctccggattcacctttagtagttactggatgcactgggtccgccaaactccaggggagggactggtgtgggtctcacggactaatgaagatgggagtatcacaaactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgtacaagagacatcggtgggcggtactcctactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [278] t78>c|c90>t|c108>t|g133>a,A45>T(- - -)|a142>g,K48>E(+ - -)|g147>a|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|g313>a,A105>T(- - -)| t78>c| c90>t|c108>t| g133>a,A45>T(- - -)|a142>g,K48>E(+ - -)|g147>a|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)| g313>a,A105>T(- - -)| 320 225 208 17 5 12 3 6 2 2 0 0 0 1 0 1 1 1 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 22 2 2 0 0 0 2 0 0 0 0 0 0 0 0 0 51 51 47 4 2 2 1 2 0 0 0 0 0 0 0 0 0 1 30 24 19 5 0 5 1 1 0 1 0 0 0 1 0 0 1 0 117 114 110 4 0 4 1 2 0 0 0 0 0 0 0 1 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcacctttagtagttactgg atgcactgggtccgccaaactccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt acaagagacatcggtgggcggtactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttagtagttactgg atgcactgggtccgccaaactccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt acaagagacatcggtgggcggtactcctac IGA1 15 213 11 4 7 9 4 6 2 2 6 5 JY8QFUQ01BGY6V 12949 productive Homsap IGHV4-31*06 F 974 93.64 206/220 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-6*01 F 1 10 7 16 10.7.16 [1.17.38.11] CARDRGPSSSSKMEYDYW in-frame - tctctggtgcctccgtcagcagtggtagtttctactggacctggattcgtcagcacccagggaagggcctggagtggattgggtacatctattacagtgggagcgcctactacaacccgtccctcaagagtcgagttgccatatcaatagacacgtctaagaaccagttctccctgaacttgagttctgtgactgtcgcggacacggccgtttattactgtgcgagagatcggggccctagcagctcgtccaaaatggaatatgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [300] g83>c,G28>A(- + -)|a88>g,I30>V(+ - +)|a110>t,Y37>F(- + -)|g119>c,S40>T(+ - +)|c126>t|c129>t|a193>g,T65>A(- - -)|a229>g,T77>A(- - -)|g238>a,V80>I(+ - +)|g270>c,K90>N(+ - -)|c271>t|c276>t|c287>t,A96>V(+ - +)|g303>t| g83>c,G28>A(- + -)|a88>g,I30>V(+ - +)|a110>t,Y37>F(- + -)| g119>c,S40>T(+ - +)|c126>t|c129>t| a193>g,T65>A(- - -)| a229>g,T77>A(- - -)|g238>a,V80>I(+ - +)|g270>c,K90>N(+ - -)|c271>t|c276>t|c287>t,A96>V(+ - +)|g303>t| 311 220 206 14 5 9 3 1 5 0 0 0 1 0 3 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 1 0 0 0 0 0 1 0 1 0 0 0 51 51 48 3 2 1 0 0 2 0 0 0 0 0 1 0 0 0 30 21 20 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 116 114 106 7 3 4 1 1 3 0 0 0 0 0 1 0 1 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,228-229(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,227-228(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|agca,131-134(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agtt,225-228(FR3)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|tacc,228-231(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tgcc,285-288(FR3)|tact,307-310(FR3)| tctct ggtgcctccgtcagcagtggtagtttctac tggacctggattcgtcagcacccagggaagggcctggagtggattgggtac atctattacagtgggagcgcc tactacaacccgtccctcaagagtcgagttgccatatcaatagacacgtctaagaaccagttctccctgaacttgagttctgtgactgtcgcggacacggccgtttattactgt gcgagagatcggggccctagcagctcgtccaaaatggaatatgactac ARDRGPSSSSKMEYDY IGHV4-31 IGHD6-6 IGHJ4 ggtgcctccgtcagcagtggtagtttctac tggacctggattcgtcagcacccagggaagggcctggagtggattgggtac atctattacagtgggagcgcc tactacaacccgtccctcaagagtcgagttgccatatcaatagacacgtctaagaaccagttctccctgaacttgagttctgtgactgtcgcggacacggccgtttattactgt gcgagagatcggggccctagcagctcgtccaaaatggaatatgactac IGA1 14 216 9 5 6 10 3 4 5 0 5 4 JY8QFUQ01BH03V 11275 productive (see comment) Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F 840 88.32 189/214 nt 87.85 188/214 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-16*02 F 1 8 7 13 8.7.13 [1.17.38.11] CTRGRSDAWELSDHW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'A' (1 nucleotides at position 21 to 21 in the user submitted sequence), (cause frameshift); cgacgatgggtcctgcagaaAactgcttctggagttggatccgccagtccccagggaaggggctggagtggattggggaggtcaatgatagaggaggcatcgactacaacccgtccctcaagagtcgagtcaccatatcattagacacgtccaacaaccaagtctccctgaggttgagctctgtgaccgccgcggacacggctgtgtattactgtacgcgagggcgatccgacgcgtgggaattatcggaccactggggccagggaaccgtggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtcagggcttcttccc NA IGA1 100 100 [285] t76>g,Y26>D(- - -)|t78>c,Y26>D(- - -)|g80>a,G27>D(- - -)|t89>g,F30>C(+ - -)|t105>a,S35>R(- - -)|g106>a,G36>N(- - -)|g107>a,G36>N(- - -)|t108>c,G36>N(- - -)|a110>g,Y37>C(- - -)|a113>t,Y38>F(- + -)|c120>t|c133>t,P45>S(+ - -)|a165>g|a166>g,I56>V(+ - +)|c172>g,H58>D(- - -)|t177>a,S59>R(- - -)|a190>g,S64>G(+ + -)|c194>t,T65>I(- - -)|a196>g,N66>D(+ + -)|g238>t,V80>L(+ - +)|g252>c,K84>N(+ - -)|g258>a|t259>g,F87>V(+ - -)|a269>g,K90>R(+ + +)|c271>t|g313>a,A105>T(- - -)|a316>c| t76>g,Y26>D(- - -)|t78>c,Y26>D(- - -)| g80>a,G27>D(- - -)|t89>g,F30>C(+ - -)|t105>a,S35>R(- - -)|g106>a,G36>N(- - -)|g107>a,G36>N(- - -)|t108>c,G36>N(- - -)|a110>g,Y37>C(- - -)|a113>t,Y38>F(- + -)| c120>t|c133>t,P45>S(+ - -)|a165>g| a166>g,I56>V(+ - +)|c172>g,H58>D(- - -)|t177>a,S59>R(- - -)|a190>g,S64>G(+ + -)|c194>t,T65>I(- - -)| a196>g,N66>D(+ + -)|g238>t,V80>L(+ - +)|g252>c,K84>N(+ - -)|g258>a|t259>g,F87>V(+ - -)|a269>g,K90>R(+ + +)|c271>t| g313>a,A105>T(- - -)|a316>c| 320 222 195 27 5 22 6 5 4 2 1 0 1 2 1 1 1 3 78 4 2 2 0 2 0 0 0 1 0 0 0 0 0 0 0 1 36 24 16 8 0 8 1 3 0 1 0 0 1 1 0 0 0 1 51 51 48 3 2 1 1 0 2 0 0 0 0 0 0 0 0 0 30 21 16 5 0 5 2 0 1 0 0 0 0 1 0 1 0 0 117 114 107 7 2 5 2 1 1 0 0 0 0 0 1 0 1 1 8 8 6 2 1 1 0 1 0 0 1 0 0 0 0 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| cgac gatgggtcctgcagaaactgcttc tggagttggatccgccagtccccagggaaggggctggagtggattggggag gtcaatgatagaggaggcatc gactacaacccgtccctcaagagtcgagtcaccatatcattagacacgtccaacaaccaagtctccctgaggttgagctctgtgaccgccgcggacacggctgtgtattactgt acgcgagggcgatccgacgcgtgggaattatcggaccac TRGRSDAWELSDH IGHV4-34 IGHD3-16 IGHJ4 gatgggtcctgcagaaactgcttc tggagttggatccgccagtccccagggaaggggctggagtggattggggag gtcaatgatagaggaggcatc gactacaacccgtccctcaagagtcgagtcaccatatcattagacacgtccaacaaccaagtctccctgaggttgagctctgtgaccgccgcggacacggctgtgtattactgt acgcgagggcgatccgacgcgtgggaattatcggaccac IGA1 23 210 15 8 8 11 7 12 4 0 6 13 JY8QFUQ01BH0GH 21416 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 849 87 194/223 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-3*01 F 3 8 8 10 8.8.10 [3.17.38.11] CASFRVLESGYW in-frame - tcaggtctctggattcacattcggtagttttatgatgaactgggtccgccaggctccagggaagggactggagtgggtcgcatcgattagccctactagtactttcatagactacgcagactcagtgaggggccggttcaccatctccagagataacgccgagaacttactgtatctgcaaatgaacggcctgagagtcgaagacacggctgtctattactgtgcgagttttcgtgtcctggaaagtggctactggggccagggaaccttggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] g70>c,A24>Q(- - -)|c71>a,A24>Q(- - -)|a72>g,A24>Q(- - -)|c74>t,A25>V(+ - +)|c87>a|a103>g,S35>G(+ + -)|c108>t|a110>t,Y37>F(- + -)|g113>t,S38>M(- - -)|c114>g,S38>M(- - -)|g147>a|t160>g,S54>A(- + -)|c165>g|t171>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g176>c,S59>T(+ - +)|g188>c,S63>T(+ - +)|a191>t,Y64>F(- + -)|t196>g,Y66>D(- - -)|a215>g,K72>R(+ + +)|a225>g|c243>t|a250>g,K84>E(+ - -)|c257>t,S86>L(- - -)|a277>g,S93>G(+ + -)|c287>t,A96>V(+ - +)|g291>a|g303>c| g70>c,A24>Q(- - -)|c71>a,A24>Q(- - -)|a72>g,A24>Q(- - -)|c74>t,A25>V(+ - +)| c87>a|a103>g,S35>G(+ + -)|c108>t|a110>t,Y37>F(- + -)|g113>t,S38>M(- - -)|c114>g,S38>M(- - -)| g147>a|t160>g,S54>A(- + -)|c165>g| t171>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g176>c,S59>T(+ - +)|g188>c,S63>T(+ - +)|a191>t,Y64>F(- + -)| t196>g,Y66>D(- - -)|a215>g,K72>R(+ + +)|a225>g|c243>t|a250>g,K84>E(+ - -)|c257>t,S86>L(- - -)|a277>g,S93>G(+ + -)|c287>t,A96>V(+ - +)|g291>a|g303>c| a318>t,R106>S(- - -)|g319>t|a320>t| 317 (320) 228 (231) 199 29 (32) 9 (11) 20 (21) 6 2 5 1 1 2 2 (4) 0 5 2 1 (2) 2 78 10 6 4 0 4 1 0 1 0 0 1 0 0 1 0 0 0 36 24 18 6 2 4 1 0 1 0 0 1 1 0 0 1 1 0 51 51 48 3 2 1 0 1 0 0 0 0 0 0 0 1 0 1 30 24 18 6 1 5 0 0 0 1 1 0 1 0 3 0 0 0 117 114 104 10 4 6 4 1 3 0 0 0 0 0 1 0 0 1 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 0 0 0 0 0 0 (2) 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tcaggtctct ggattcacattcggtagttttatg atgaactgggtccgccaggctccagggaagggactggagtgggtcgcatcg attagccctactagtactttcata gactacgcagactcagtgaggggccggttcaccatctccagagataacgccgagaacttactgtatctgcaaatgaacggcctgagagtcgaagacacggctgtctattactgt gcgagttttcgtgtcctggaaagtggctac ASFRVLESGY IGHV3-21 IGHD3-3 IGHJ4 ggattcacattcggtagttttatg atgaactgggtccgccaggctccagggaagggactggagtgggtcgcatcg attagccctactagtactttcata gactacgcagactcagtgaggggccggttcaccatctccagagataacgccgagaacttactgtatctgcaaatgaacggcctgagagtcgaagacacggctgtctattactgt gcgagttttcgtgtcctggaaagtggctac IGA1 25 213 12 13 6 14 6 11 6 3 7 9 JY8QFUQ01BH1QX 15165 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 837 87.56 190/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-15*01 F 1 8 8 11 8.8.11 [1.17.38.11] CARDGEWEMLDHW in-frame + ctccggattcaccttcagtaggtattggatgtattgggtccgccaagctccaggcaaggggctggtgtgggtctcacgcattaaaagtgatgggacgagtgcaacctacgcggactccgtgaagggccgattcaccacctccagagacaacgccaaggaaacgatgtatctgcacatgaacagcctgagagtcgacgacacggcgacatatttttgtgcaagagacggggagtgggagatgcttgaccactggggccagggagccctggtcaccgtctcctcagcatcccctgaccagccccaaggtcttcccgctgcagctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 86 79 [282] t78>c|c108>g,S36>R(- - -)|c111>t|c118>t,H40>Y(+ - -)|c120>t,H40>Y(+ - -)|g141>c|t165>c|t171>a,N57>K(+ - -)|g188>c,S63>T(+ - +)|t189>g,S63>T(+ - +)|c192>t|a193>g,T65>A(- - -)|g197>c,S66>T(+ - +)|t233>c,I78>T(- - -)|a253>g,N85>E(+ - -)|c255>a,N85>E(+ - -)|c259>a,L87>M(+ + -)|a270>c,Q90>H(- + -)|t279>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t300>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| t78>c| c108>g,S36>R(- - -)|c111>t| c118>t,H40>Y(+ - -)|c120>t,H40>Y(+ - -)|g141>c|t165>c| t171>a,N57>K(+ - -)|g188>c,S63>T(+ - +)|t189>g,S63>T(+ - +)|c192>t|a193>g,T65>A(- - -)| g197>c,S66>T(+ - +)|t233>c,I78>T(- - -)|a253>g,N85>E(+ - -)|c255>a,N85>E(+ - -)|c259>a,L87>M(+ + -)|a270>c,Q90>H(- + -)|t279>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t300>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| 320 225 198 27 7 20 2 2 6 5 1 2 1 1 4 1 0 2 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 1 0 0 0 0 0 0 1 0 0 51 51 47 4 2 2 0 0 2 1 0 0 0 0 1 0 0 0 30 24 19 5 1 4 1 0 1 0 0 0 0 1 1 0 0 1 117 114 99 15 2 13 1 2 2 3 1 2 1 0 2 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcaccttcagtaggtattgg atgtattgggtccgccaagctccaggcaaggggctggtgtgggtctcacgc attaaaagtgatgggacgagtgca acctacgcggactccgtgaagggccgattcaccacctccagagacaacgccaaggaaacgatgtatctgcacatgaacagcctgagagtcgacgacacggcgacatatttttgt gcaagagacggggagtgggagatgcttgaccac ARDGEWEMLDH IGHV3-74 IGHD2-15 IGHJ4 ggattcaccttcagtaggtattgg atgtattgggtccgccaagctccaggcaaggggctggtgtgggtctcacgc attaaaagtgatgggacgagtgca acctacgcggactccgtgaagggccgattcaccacctccagagacaacgccaaggaaacgatgtatctgcacatgaacagcctgagagtcgacgacacggcgacatatttttgt gcaagagacggggagtgggagatgcttgaccac IGA2 26 213 14 12 8 15 6 11 4 2 15 5 JY8QFUQ01BH2A1 19503 productive Homsap IGHV1-2*02 F 921 92.52 198/214 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD4-23*01 ORF 1 8 8 12 8.8.12 [0.17.38.11] CATDVPAGQLWGYW in-frame - tggatacacattcaccgacaactatatgcactgggtgcggcaggcccctggacaggggcttgagtggatgggatggatcgaacctaacagtggagacacaaactatgcacaaaagtttcagggcagggtcaccatgacgagggacacgtccatcgccacagcctacatggagttgagcaggctgagagctgacgacacggccgtgtactactgtgcgaccgacgtccccgcagggcaactctggggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [281] c87>a|g107>a,G36>D(- - -)|t109>a,Y37>N(- - -)|a129>g|a144>g|a169>g,N57>E(+ - -)|c171>a,N57>E(+ - -)|t189>a|g191>a,G64>D(- - -)|g207>a|c237>g|a253>g,S85>A(- + -)|g254>c,S85>A(- + -)|c271>t|t286>g,S96>A(- + -)|t306>c| c87>a|g107>a,G36>D(- - -)|t109>a,Y37>N(- - -)| a129>g|a144>g| a169>g,N57>E(+ - -)|c171>a,N57>E(+ - -)|t189>a|g191>a,G64>D(- - -)| g207>a|c237>g|a253>g,S85>A(- + -)|g254>c,S85>A(- + -)|c271>t|t286>g,S96>A(- + -)|t306>c| g317>c,R106>T(- - -)|a318>c,R106>T(- - -)| 316 (320) 218 (222) 202 (204) 16 (18) 8 8 (10) 4 3 1 1 0 (1) 2 0 2 1 (2) 1 0 1 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 0 0 0 1 0 1 0 0 0 0 51 51 49 2 2 0 2 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 1 1 0 0 0 1 0 1 0 0 0 0 117 114 107 7 4 3 1 1 1 1 0 0 0 0 1 1 0 1 4 (8) 4 4 (6) 0 (2) 0 0 (2) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatacacattcaccgacaactat atgcactgggtgcggcaggcccctggacaggggcttgagtggatgggatgg atcgaacctaacagtggagacaca aactatgcacaaaagtttcagggcagggtcaccatgacgagggacacgtccatcgccacagcctacatggagttgagcaggctgagagctgacgacacggccgtgtactactgt gcgaccgacgtccccgcagggcaactctggggctac ATDVPAGQLWGY IGHV1-2 IGHD4-23 IGHJ4 ggatacacattcaccgacaactat atgcactgggtgcggcaggcccctggacaggggcttgagtggatgggatgg atcgaacctaacagtggagacaca aactatgcacaaaagtttcagggcagggtcaccatgacgagggacacgtccatcgccacagcctacatggagttgagcaggctgagagctgacgacacggccgtgtactactgt gcgaccgacgtccccgcagggcaactctggggctac IGA1 16 213 9 7 4 8 5 8 6 2 3 5 JY8QFUQ01BH7NG 12255 productive Homsap IGHV3-66*02 F 948 93.93 201/214 nt NA Homsap IGHJ3*01 F 232 96 48/50 nt Homsap IGHD6-13*01 F 1 8 7 16 8.7.16 [1.17.38.11] CARDSPYSSGWSDGFDVW in-frame + ctctggattcaccgtcagagataactacgtgacctgggtccgccaggctccagggaaggggctggagtgggtctcaattatttatatcagtggtagcacatactacgcagactccgtgaagggccgattcaccatctccagagacaattcgaagaacacggtgtatcttcagatgaacagtctgagagctgaggacacggctgtgtattactgtgcgagagatagtccatatagcagcggctggtctgatggctttgatgtctggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgctggtccagggcttcttccc NA IGA1 93 95 [293] t105>a,S35>R(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|g163>a,V55>I(+ - +)|g173>t,S58>I(- - -)|g175>a,G59>S(+ + -)|c249>g|c259>g,L87>V(+ - +)|a270>g|c279>t| t105>a,S35>R(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)| a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|g163>a,V55>I(+ - +)| g173>t,S58>I(- - -)|g175>a,G59>S(+ + -)| c249>g|c259>g,L87>V(+ - +)|a270>g|c279>t| 318 220 207 13 3 10 3 3 2 0 0 0 0 1 1 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 1 0 0 0 0 1 0 0 0 0 51 51 48 3 0 3 1 1 0 0 0 0 0 0 1 0 0 0 30 21 19 2 0 2 0 1 0 0 0 0 0 0 0 0 1 0 117 114 110 4 3 1 1 0 1 0 0 0 0 0 0 2 0 0 6 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccgtcagagataactac gtgacctgggtccgccaggctccagggaaggggctggagtgggtctcaatt atttatatcagtggtagcaca tactacgcagactccgtgaagggccgattcaccatctccagagacaattcgaagaacacggtgtatcttcagatgaacagtctgagagctgaggacacggctgtgtattactgt gcgagagatagtccatatagcagcggctggtctgatggctttgatgtc ARDSPYSSGWSDGFDV IGHV3-66 IGHD6-13 IGHJ3 ggattcaccgtcagagataactac gtgacctgggtccgccaggctccagggaaggggctggagtgggtctcaatt atttatatcagtggtagcaca tactacgcagactccgtgaagggccgattcaccatctccagagacaattcgaagaacacggtgtatcttcagatgaacagtctgagagctgaggacacggctgtgtattactgt gcgagagatagtccatatagcagcggctggtctgatggctttgatgtc IGA1 13 210 8 5 5 9 3 4 3 0 4 6 JY8QFUQ01BHCZE 21649 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 788 84.86 185/218 nt NA Homsap IGHJ4*02 F (see comment) 186 87.5 42/48 nt Homsap IGHD3-10*02 F 2 8 8 13 8.8.13 [1.17.38.11] CAKERDNNVLPVDSW in-frame + Low V-REGION identity (84,86% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) cctctggattcagcttcaatagttatactttgagttgggtccgccaggctccagggaaggggctggagtgggtctcagctatcagtggaacaggtgaaacaaccttctacgcggactccgtgaggggccggttcaccgtctccagagacaatttcaagaatactctgtacttgcaattgagcgacctgagagccgaagacacggccgtctattactgtgcgaaggagagagataacaatgttctgcccgttgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [288] c86>g,T29>S(+ - +)|t90>c|g104>a,S35>N(- - -)|c105>t,S35>N(- - -)|c108>t|g112>a,A38>T(- - -)|c114>t,A38>T(- - -)|a115>t,M39>L(+ + -)|c120>t|t168>c|t174>a|g176>c,S59>T(+ - +)|t177>a,S59>T(+ - +)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)|a195>c|a197>t,Y66>F(- + -)|a204>g|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|c248>t,S83>F(- - -)|c255>t|g258>t|t264>c|c265>t|a271>t,M91>L(+ + -)|a275>g,N92>S(- - -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g291>a|a303>c|a318>g| c86>g,T29>S(+ - +)|t90>c|g104>a,S35>N(- - -)|c105>t,S35>N(- - -)|c108>t|g112>a,A38>T(- - -)|c114>t,A38>T(- - -)| a115>t,M39>L(+ + -)|c120>t| t168>c|t174>a|g176>c,S59>T(+ - +)|t177>a,S59>T(+ - +)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)|a195>c| a197>t,Y66>F(- + -)|a204>g|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|c248>t,S83>F(- - -)|c255>t|g258>t|t264>c|c265>t|a271>t,M91>L(+ + -)|a275>g,N92>S(- - -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g291>a|a303>c| a318>g| 320 226 192 34 14 20 6 5 7 3 2 1 3 3 2 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 2 5 0 2 3 1 0 0 0 0 0 1 0 0 51 51 49 2 1 1 0 0 1 0 0 0 1 0 0 0 0 0 30 24 15 9 3 6 0 1 0 1 1 1 0 3 2 0 0 0 117 114 99 15 7 8 5 2 3 1 1 0 2 0 0 0 1 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcagcttcaatagttatact ttgagttgggtccgccaggctccagggaaggggctggagtgggtctcagct atcagtggaacaggtgaaacaacc ttctacgcggactccgtgaggggccggttcaccgtctccagagacaatttcaagaatactctgtacttgcaattgagcgacctgagagccgaagacacggccgtctattactgt gcgaaggagagagataacaatgttctgcccgttgactcc AKERDNNVLPVDS IGHV3-23 IGHD3-10 IGHJ4 ggattcagcttcaatagttatact ttgagttgggtccgccaggctccagggaaggggctggagtgggtctcagct atcagtggaacaggtgaaacaacc ttctacgcggactccgtgaggggccggttcaccgtctccagagacaatttcaagaatactctgtacttgcaattgagcgacctgagagccgaagacacggccgtctattactgt gcgaaggagagagataacaatgttctgcccgttgactcc IGA1 33 213 20 13 12 17 8 16 8 5 9 11 JY8QFUQ01BHGDW 11078 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 918 91.71 199/217 nt NA Homsap IGHJ5*02 F 237 96.08 49/51 nt Homsap IGHD6-6*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVRSEYSSSNWFDPW in-frame - ctctggattccccatcagtggctttagaatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattgatagttttagtcagaacatatactacagagactcagtgaggggccgattcaccatctccagagacaacgccaggaactcattgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgtgagatccgagtatagcagttcaaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] a85>c,T29>P(+ + -)|t88>a,F30>I(+ - -)|a106>g,S36>G(+ + -)|a110>t,Y37>F(- + -)|c114>a,S38>R(- - -)|a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a175>t,S59>F(- - -)|g176>t,S59>F(- - -)|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>g,S63>Q(- - -)|t190>a,Y64>N(- - -)|g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|a215>g,K72>R(+ + +)|a251>g,K84>R(+ + +)|c259>t|c314>t,A105>V(+ - +)| a85>c,T29>P(+ + -)|t88>a,F30>I(+ - -)|a106>g,S36>G(+ + -)|a110>t,Y37>F(- + -)|c114>a,S38>R(- - -)| a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a175>t,S59>F(- - -)|g176>t,S59>F(- - -)|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>g,S63>Q(- - -)|t190>a,Y64>N(- - -)| g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|a215>g,K72>R(+ + +)|a251>g,K84>R(+ + +)|c259>t| c314>t,A105>V(+ - +)|g319>t|a320>c| 318 (320) 223 (225) 204 19 (21) 1 (3) 18 4 3 2 0 2 (3) 1 2 2 0 1 1 (2) 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 0 0 0 1 1 1 1 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 16 8 0 8 1 2 0 0 1 0 1 1 0 0 1 1 117 114 109 5 1 4 2 1 1 0 0 0 0 0 0 1 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattccccatcagtggctttaga atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attgatagttttagtcagaacata tactacagagactcagtgaggggccgattcaccatctccagagacaacgccaggaactcattgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gtgagatccgagtatagcagttcaaactggttcgacccc VRSEYSSSNWFDP IGHV3-21 IGHD6-6 IGHJ5 ggattccccatcagtggctttaga atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attgatagttttagtcagaacata tactacagagactcagtgaggggccgattcaccatctccagagacaacgccaggaactcattgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gtgagatccgagtatagcagttcaaactggttcgacccc IGA1 18 213 8 10 4 7 4 11 1 0 4 13 JY8QFUQ01BHITL 11677 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 788 84.86 185/218 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-10*01 F 2 8 8 15 8.8.15 [1.17.38.11] CVRVKRFGSGNVLFDYW in-frame - Low V-REGION identity (84,86% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release gctccggtttcacgtttgacaactatgccatgacttgggtccgccagactccagggaaggggctgcagtggctctcaactattactgcttatgggactctcacatactacgctgcctccgtgaagggccggttcaccctctccagggacaactccaacaacacggtgtatctgcaaatggacagtctgagagccgaagacacggccgtattttactgtgtgagagttaaacggttcggttcggggaatgtactctttgactactggggccagggaaccctggtcaccgtctcgtcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [294, 293] c74>g|t78>c|a81>t|c87>g|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g133>a,A45>T(- - -)|g151>c,E51>Q(+ + -)|g157>c,V53>L(+ - +)|g163>a,A55>T(- - -)|g170>c,S57>T(+ - +)|g173>c,G58>A(- + -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|t186>g|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|a190>c,S64>L(- - -)|g191>t,S64>L(- - -)|a204>t|a206>c,D69>A(- - -)|a232>c,I78>L(+ + +)|a240>g|t246>c|g252>c,K84>N(+ - -)|c259>g,L87>V(+ - +)|a274>g,N92>D(+ + -)|c279>t|g291>a|a305>t,Y102>F(- + -)|c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)| c74>g|t78>c| a81>t|c87>g|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g133>a,A45>T(- - -)|g151>c,E51>Q(+ + -)|g157>c,V53>L(+ - +)|g163>a,A55>T(- - -)| g170>c,S57>T(+ - +)|g173>c,G58>A(- + -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|t186>g|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|a190>c,S64>L(- - -)|g191>t,S64>L(- - -)| a204>t|a206>c,D69>A(- - -)|a232>c,I78>L(+ + +)|a240>g|t246>c|g252>c,K84>N(+ - -)|c259>g,L87>V(+ - +)|a274>g,N92>D(+ + -)|c279>t|g291>a|a305>t,Y102>F(- + -)| c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)|a320>t| 319 (320) 225 (226) 190 35 (36) 10 (11) 25 4 7 3 2 3 0 4 (5) 0 7 3 1 1 78 5 3 2 2 0 0 0 0 1 0 0 0 0 0 1 0 0 36 24 19 5 2 3 1 2 0 0 0 0 1 0 0 1 0 0 51 51 45 6 0 6 0 2 1 0 0 0 0 0 3 0 0 0 30 24 15 9 1 8 0 2 0 0 1 0 1 0 3 0 1 1 117 114 103 11 5 6 2 1 1 1 2 0 2 0 1 1 0 0 7 (8) 7 5 2 (3) 0 (1) 2 1 0 1 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gctcc ggtttcacgtttgacaactatgcc atgacttgggtccgccagactccagggaaggggctgcagtggctctcaact attactgcttatgggactctcaca tactacgctgcctccgtgaagggccggttcaccctctccagggacaactccaacaacacggtgtatctgcaaatggacagtctgagagccgaagacacggccgtattttactgt gtgagagttaaacggttcggttcggggaatgtactctttgactac VRVKRFGSGNVLFDY IGHV3-23 IGHD3-10 IGHJ4 ggtttcacgtttgacaactatgcc atgacttgggtccgccagactccagggaaggggctgcagtggctctcaact attactgcttatgggactctcaca tactacgctgcctccgtgaagggccggttcaccctctccagggacaactccaacaacacggtgtatctgcaaatggacagtctgagagccgaagacacggccgtattttactgt gtgagagttaaacggttcggttcggggaatgtactctttgactac IGA2 31 213 13 18 9 19 4 12 5 3 12 11 JY8QFUQ01BHN1C 10050 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 885 90.65 194/214 nt 90.19 193/214 nt Homsap IGHJ5*02 F 228 94.12 48/51 nt Homsap IGHD4-17*01 F 2 8 7 15 8.7.15 [1.17.37.11] CAKNNNDFGDYDWFDPW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 68 of V-REGION: 1 nucleotides (from position 110 in the user submitted sequence), (cause frameshift) tctctggtgtctccatcagtagttcctactggagttggatccggcagcccccagggaagggcctggagtggattggttatgtctattatactgggggcaccgactccaaccctccctcaagagtcgtgtcaccctgtcaatggacacgtccaagaaccagttctccttggaactgagctctgtgaccgctgcggacacggccgtctattactgtgcgaaaaacaacaatgacttcggtgactacgactggttcgacccctggggccagggaatgctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [290] g83>t,G28>V(- - -)|a110>c,Y37>S(+ - -)|c120>t|a147>c|g162>t|a166>g,I56>V(+ - +)|c174>t|g176>c,S59>T(+ - +)|a190>g,S64>G(+ + -)|a196>g,N66>D(+ + -)|a200>c,Y67>S(+ - -)|a225>t|a232>c,I78>L(+ + +)|a234>g,I78>L(+ + +)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|c265>t|a268>g,K90>E(+ - -)|g270>a,K90>E(+ - -)|g303>c| g83>t,G28>V(- - -)|a110>c,Y37>S(+ - -)| c120>t|a147>c|g162>t| a166>g,I56>V(+ - +)|c174>t|g176>c,S59>T(+ - +)|a190>g,S64>G(+ + -)| a196>g,N66>D(+ + -)|a200>c,Y67>S(+ - -)|a225>t|a232>c,I78>L(+ + +)|a234>g,I78>L(+ + +)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|c265>t|a268>g,K90>E(+ - -)|g270>a,K90>E(+ - -)|g303>c| g317>a,R106>K(+ + +)|g319>a| 316 (320) 218 (222) 198 (200) 20 (22) 7 (8) 13 (14) 6 2 (4) 3 0 4 0 1 0 2 0 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 1 0 0 0 0 0 1 0 51 51 48 3 3 0 0 0 1 0 1 0 0 0 0 0 1 0 30 21 17 4 1 3 2 0 1 0 0 0 0 0 1 0 0 0 117 113 102 11 3 8 4 2 1 0 2 0 1 0 1 0 0 0 4 (8) 4 4 (6) 0 (2) 0 (1) 0 (1) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgtctccatcagtagttcctac tggagttggatccggcagcccccagggaagggcctggagtggattggttat gtctattatactgggggcacc gactccaaccctccctcaagagtcgtgtcaccctgtcaatggacacgtccaagaaccagttctccttggaactgagctctgtgaccgctgcggacacggccgtctattactgt gcgaaaaacaacaatgacttcggtgactacgactggttcgacccc AKNNNDFGDYDWFDP IGHV4-59 IGHD4-17 IGHJ5 ggtgtctccatcagtagttcctac tggagttggatccggcagcccccagggaagggcctggagtggattggttat gtctattatactgggggcacc gactccaaccctccctcaagagtcgtgtcaccctgtcaatggacacgtccaagaaccagttctccttggaactgagctctgtgaccgctgcggacacggccgtctattactgt gcgaaaaacaacaatgacttcggtgactacgactggttcgacccc IGA1 20 209 11 9 5 9 6 11 6 1 8 5 JY8QFUQ01BI347 18445 productive (see comment) Homsap IGHV4-4*02 F, or Homsap IGHV4-4*03 F or Homsap IGHV4-4*04 F or Homsap IGHV4-4*05 F 824 86.7 189/218 nt 85.78 187/218 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-22*01 F 2 9 7 15 9.7.15 [1.17.38.11] CTSREHAFSGQFTGDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 35 of V-REGION: 'CGT' (3 nucleotides at position 23 to 25 in the user submitted sequence), (do not cause frameshift);in FR2-IMGT at codon 44 of V-REGION: 'TG' (2 nucleotides at position 53 to 54 in the user submitted sequence), (cause frameshift); tgtctggtgtctccgtcaccagCGTcagtcactggtggacctgggtccgccaTGgcccccagggaagggactggagtggattggagaaatctattattatggcatcaccaatttcaacccgtccctcaagagtcgaatcagcatgtcagtggacgagtccaagaaccagttctccctgagactgacttctgttaccgccgcggacacggccgtttattattgtacgagtcgcgaacatgcttttagtggtcaattcacgggtgactactggggccagggaaccctgatgaccgtcacctcagcctccaccaaaggggccccatcggtcttcccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 88 [304, 303] c75>g|g83>t,G28>V(- - -)|a88>g,I30>V(+ - +)|g92>c,S31>T(+ - +)|t105>c|a109>c,N37>H(- - -)|g119>c,S40>T(+ - +)|t120>c,S40>T(+ - +)|g147>a|g162>a|c172>t,H58>Y(+ - -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|g189>c|g191>t,S64>I(- - -)|c198>t|a200>t,Y67>F(- + -)|g226>a,V76>I(+ - +)|c230>g,T77>S(+ - +)|a234>g,I78>M(+ + -)|a240>g|a244>g,K82>E(+ - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g282>t|g303>t|c309>t|g313>a,A105>T(- - -)| c75>g| g83>t,G28>V(- - -)|a88>g,I30>V(+ - +)|g92>c,S31>T(+ - +)|t105>c|a109>c,N37>H(- - -)| g119>c,S40>T(+ - +)|t120>c,S40>T(+ - +)|g147>a|g162>a| c172>t,H58>Y(+ - -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|g189>c|g191>t,S64>I(- - -)| c198>t|a200>t,Y67>F(- + -)|g226>a,V76>I(+ - +)|c230>g,T77>S(+ - +)|a234>g,I78>M(+ + -)|a240>g|a244>g,K82>E(+ - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g282>t|g303>t|c309>t| g313>a,A105>T(- - -)|a318>t,R106>S(- - -)|g319>c|a320>g| 317 (320) 223 (226) 193 30 (33) 10 (12) 20 (21) 5 (6) 6 4 2 1 0 2 (3) 0 4 (5) 2 4 0 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 27 22 5 1 4 1 0 0 1 1 0 0 0 1 0 1 0 51 51 47 4 2 2 0 2 0 1 0 0 0 0 1 0 0 0 30 21 16 5 1 4 0 1 1 0 0 0 1 0 1 0 1 0 117 114 100 14 5 9 4 2 3 0 0 0 1 0 1 1 2 0 5 (8) 5 4 1 (4) 0 (2) 1 (2) 0 (1) 1 0 0 0 0 0 (1) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tgtct ggtgtctccgtcaccagcagtcactgg tggacctgggtccgccagcccccagggaagggactggagtggattggagaa atctattattatggcatcacc aatttcaacccgtccctcaagagtcgaatcagcatgtcagtggacgagtccaagaaccagttctccctgagactgacttctgttaccgccgcggacacggccgtttattattgt acgagtcgcgaacatgcttttagtggtcaattcacgggtgactac TSREHAFSGQFTGDY IGHV4-4 IGHD3-22 IGHJ4 ggtgtctccgtcaccagcagtcactgg tggacctgggtccgccagcccccagggaagggactggagtggattggagaa atctattattatggcatcacc aatttcaacccgtccctcaagagtcgaatcagcatgtcagtggacgagtccaagaaccagttctccctgagactgacttctgttaccgccgcggacacggccgtttattattgt acgagtcgcgaacatgcttttagtggtcaattcacgggtgactac IGG2 28 213 16 12 9 18 7 10 7 2 11 8 JY8QFUQ01BIGLC 5263 productive (see comment) Homsap IGHV3-48*01 F, or Homsap IGHV3-48*02 F 994 95.83 207/216 nt 95.37 206/216 nt Homsap IGHJ6*02 F 256 90.32 56/62 nt Homsap IGHD4-23*01 ORF 2 8 8 20 8.8.20 [1.16.38.11] CVRGDYGGAPGHYYDPYGLDVW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 47 of V-REGION: 1 nucleotides (from position 55 in the user submitted sequence), (cause frameshift) ctctggattcaccttcaggagttatatcatgaactgggtccgccaggctccaggaaggggctggagtggatttcatacattagtagtagtggtattatcatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgtgtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtctggggccaagggaccacggtcaaagtctcctcagcctccaaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttgccccgaaccggtgacggtgtcgtggaactc NA IGG1 93 94 [309] t105>g,S35>R(- - -)|c108>t|g113>t,S38>I(- - -)|g157>a,V53>I(+ - +)|a184>g,S62>G(+ + -)|g188>t,S63>I(- - -)|c191>t,T64>I(- - -)|c287>t,A96>V(+ - +)|c309>t|c314>t,A105>V(+ - +)| t105>g,S35>R(- - -)|c108>t|g113>t,S38>I(- - -)| g157>a,V53>I(+ - +)| a184>g,S62>G(+ + -)|g188>t,S63>I(- - -)|c191>t,T64>I(- - -)| c287>t,A96>V(+ - +)|c309>t| c314>t,A105>V(+ - +)|a320>g| 319 (320) 223 (224) 213 10 (11) 2 (3) 8 1 (2) 1 5 0 0 0 0 0 0 0 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 1 0 0 0 0 0 0 0 1 1 51 50 49 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 1 0 0 0 0 0 0 0 1 0 117 114 112 2 1 1 0 0 2 0 0 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcaggagttatatc atgaactgggtccgccaggctccaggaaggggctggagtggatttcatac attagtagtagtggtattatcata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgt gtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtc VRGDYGGAPGHYYDPYGLDV IGHV3-48 IGHD4-23 IGHJ6 ggattcaccttcaggagttatatc atgaactgggtccgccaggctccaggaaggggctggagtggatttcatac attagtagtagtggtattatcata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgt gtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtc IGG1 9 212 6 3 5 7 1 2 1 1 2 5 JY8QFUQ01BIHI6 19997 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 1013 96.33 210/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD4-23*01 ORF 2 8 8 14 8.8.14 [1.17.38.11] CAKDRVYGGGEPLDHW in-frame + cctctggattcaccttcagtagttataacatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagttatatcatatgatggaagaaataaatactatgcagactccgtgaagggccgactcaccatctccagagacaattccaagaacatgttgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtattactgtgcgaaagatcgggtctacggagggggcgagccgcttgaccactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [292] c108>t|g112>a,G38>N(- - -)|g113>a,G38>N(- - -)|t189>a,S63>R(- - -)|t226>c,F76>L(+ - -)|c257>t,T86>M(- - -)|c259>t|g286>c,A96>P(- - -)|g317>a,R106>K(+ + +)| c108>t|g112>a,G38>N(- - -)|g113>a,G38>N(- - -)| t189>a,S63>R(- - -)| t226>c,F76>L(+ - -)|c257>t,T86>M(- - -)|c259>t|g286>c,A96>P(- - -)| g317>a,R106>K(+ + +)| 320 226 217 9 2 7 0 3 3 1 0 0 0 1 1 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 2 1 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 23 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 117 114 110 4 1 3 0 0 2 1 0 0 0 0 1 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtagttataac atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcatatgatggaagaaataaa tactatgcagactccgtgaagggccgactcaccatctccagagacaattccaagaacatgttgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtattactgt gcgaaagatcgggtctacggagggggcgagccgcttgaccac AKDRVYGGGEPLDH IGHV3-30 IGHD4-23 IGHJ4 ggattcaccttcagtagttataac atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcatatgatggaagaaataaa tactatgcagactccgtgaagggccgactcaccatctccagagacaattccaagaacatgttgtatctgcaaatgaacagcctgagacctgaggacacggctgtgtattactgt gcgaaagatcgggtctacggagggggcgagccgcttgaccac IGG2 8 213 6 2 5 6 1 2 1 1 3 3 JY8QFUQ01BIM6Q 10251 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 1071 99.54 216/217 nt NA Homsap IGHJ2*01 F 256 98.11 52/53 nt Homsap IGHD2-15*01 F 2 8 8 19 8.8.19 [1.17.38.11] CARLHGYCGGGYCYDWYFDLW in-frame + ttctggatacagctttaccagctactggatcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatcatctatcctggtgactctgattccagatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcagcaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatgtattactgtgcgagactccatgggtattgtggtggtggttattgctacgactggtacttcgatctctggggccgtggcaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA1 71 100 [305] a193>t,T65>S(+ - +)| a193>t,T65>S(+ - +)| a320>t| 319 (320) 224 (225) 223 1 (2) 0 (1) 1 0 0 0 0 0 0 1 (2) 0 0 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 23 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ttct ggatacagctttaccagctactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgattcc agatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcagcaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatgtattactgt gcgagactccatgggtattgtggtggtggttattgctacgactggtacttcgatctc ARLHGYCGGGYCYDWYFDL IGHV5-51 IGHD2-15 IGHJ2 ggatacagctttaccagctactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgattcc agatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcagcaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatgtattactgt gcgagactccatgggtattgtggtggtggttattgctacgactggtacttcgatctc IGA1 1 213 0 1 0 0 0 1 0 0 0 1 JY8QFUQ01BISS5 12422 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 733 82.41 178/216 nt 81.48 176/216 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-6*01 F 1 7 8 13 7.8.13 [1.17.38.11] CAKDQYGSPSDFDNW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (82,41% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR2-IMGT at codon 57 of V-REGION: 'GGA' (3 nucleotides at position 82 to 84 in the user submitted sequence), (do not cause frameshift); in CDR1-IMGT, from codon 35 of V-REGION: 1 nucleotides (from position 19 in the user submitted sequence), (cause frameshift) ctcagggttcgttttgagaaatacgccatgagttgggtccgccaggctcccggaaaggggctggagtgggtctcggctattGGAggtgttgatgatgttggcacatactacgcagcctccgtgaagggtcggttcaccatatccagagacgattccagggagattctctatctacaaatgagtaacctgagagtcgacgatacggccgtctattactgtgcgaaagatcaatatgggagcccatccgactttgacaactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttctt NA IGA1 100 100 [289] t78>a|a81>g|a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|c87>t,T29>V(- - -)|t90>g,F30>L(+ - -)|g107>a,S36>K(- - -)|c108>a,S36>K(- - -)|t111>c|c120>t|a138>c|g141>a|a162>g|a169>g,S57>G(+ + -)|g173>t,G58>V(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g185>a,G62>D(- - -)|g188>t,G63>V(- - -)|a190>g,S64>G(+ + -)|a206>c,D69>A(- - -)|c222>t|c234>a|a244>g,N82>D(+ + -)|a251>g,K84>R(+ + +)|a253>g,N85>E(+ - -)|c255>g,N85>E(+ - -)|c257>t,T86>I(- - -)|g258>t,T86>I(- - -)|g261>c|g267>a|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c294>t|a303>c| t78>a| a81>g|a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|c87>t,T29>V(- - -)|t90>g,F30>L(+ - -)|g107>a,S36>K(- - -)|c108>a,S36>K(- - -)|t111>c| c120>t|a138>c|g141>a|a162>g| a169>g,S57>G(+ + -)|g173>t,G58>V(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g185>a,G62>D(- - -)|g188>t,G63>V(- - -)|a190>g,S64>G(+ + -)| a206>c,D69>A(- - -)|c222>t|c234>a|a244>g,N82>D(+ + -)|a251>g,K84>R(+ + +)|a253>g,N85>E(+ - -)|c255>g,N85>E(+ - -)|c257>t,T86>I(- - -)|g258>t,T86>I(- - -)|g261>c|g267>a|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c294>t|a303>c| 320 224 186 38 13 25 10 6 8 1 3 2 0 1 2 1 3 1 78 4 3 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 23 15 8 2 6 2 1 2 1 0 1 0 0 0 0 0 1 51 51 47 4 4 0 1 1 1 0 1 0 0 0 0 0 0 0 30 24 17 7 0 7 3 2 0 0 0 0 0 0 0 0 2 0 117 114 96 18 6 12 4 2 5 0 2 1 0 0 2 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctca gggttcgttttgagaaatacgcc atgagttgggtccgccaggctcccggaaaggggctggagtgggtctcggct attggtgttgatgatgttggcaca tactacgcagcctccgtgaagggtcggttcaccatatccagagacgattccagggagattctctatctacaaatgagtaacctgagagtcgacgatacggccgtctattactgt gcgaaagatcaatatgggagcccatccgactttgacaac AKDQYGSPSDFDN IGHV3-23 IGHD6-6 IGHJ4 gggttcgttttgagaaatacgcc atgagttgggtccgccaggctcccggaaaggggctggagtgggtctcggct attggtgttgatgatgttggcaca tactacgcagcctccgtgaagggtcggttcaccatatccagagacgattccagggagattctctatctacaaatgagtaacctgagagtcgacgatacggccgtctattactgt gcgaaagatcaatatgggagcccatccgactttgacaac IGA1 37 212 25 12 14 22 11 15 10 2 12 13 JY8QFUQ01BIVV0 21445 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 932 91.74 200/218 nt 91.28 199/218 nt Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD3-10*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKKYHYGSGTYLYYFDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'C' (1 nucleotides at position 21 to 21 in the user submitted sequence), (cause frameshift); cctctggattcacctttagcCacttttgccgtgacctgggtccgccaggctccagggaagggtctggaatgggtctcaactattagcggtagtgatggtagcaagtactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacaccctatatctgcaaatgaccagcctgagagccgaggacacggccgtatatttctgcgcgaagaagtatcactatggttcggggacttacttgtactattttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [301] g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|a110>t,Y37>F(- + -)|a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|g147>t|g153>a|g163>a,A55>T(- - -)|t171>c|g185>a,G62>D(- - -)|c194>a,T65>K(- - -)|a195>g,T65>K(- - -)|a215>g,K72>R(+ + +)|g258>c|g261>a|a275>c,N92>T(- + -)|a308>t,Y103>F(- + -)|t312>c| g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|a110>t,Y37>F(- + -)| a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|g147>t|g153>a|g163>a,A55>T(- - -)| t171>c|g185>a,G62>D(- - -)|c194>a,T65>K(- - -)|a195>g,T65>K(- - -)| a215>g,K72>R(+ + +)|g258>c|g261>a|a275>c,N92>T(- + -)|a308>t,Y103>F(- + -)|t312>c| a318>g|g319>a| 317 (320) 223 (226) 205 (206) 18 (20) 6 (8) 12 3 (4) 4 (5) 1 2 1 1 2 0 3 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 1 0 0 0 1 0 1 0 0 0 51 51 46 5 2 3 1 2 0 0 0 0 0 0 1 0 1 0 30 24 20 4 1 3 1 1 0 1 0 1 0 0 0 0 0 0 117 114 108 6 3 3 1 1 0 1 1 0 1 0 1 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagcacttttgcc gtgacctgggtccgccaggctccagggaagggtctggaatgggtctcaact attagcggtagtgatggtagcaag tactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacaccctatatctgcaaatgaccagcctgagagccgaggacacggccgtatatttctgc gcgaagaagtatcactatggttcggggacttacttgtactattttgactac AKKYHYGSGTYLYYFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcacctttagcacttttgcc gtgacctgggtccgccaggctccagggaagggtctggaatgggtctcaact attagcggtagtgatggtagcaag tactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacaccctatatctgcaaatgaccagcctgagagccgaggacacggccgtatatttctgc gcgaagaagtatcactatggttcggggacttacttgtactattttgactac IGA2 18 213 10 8 5 10 5 8 5 1 6 6 JY8QFUQ01BIWEF 1497 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [1.17.38.11] CAKDGARGGGSCFNSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctacattcgcctttagcaactatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtgtcagatattagtgggagtggtgttagcacatactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgtgcgaaagatggcgctagaggtggcggcagttgctttaactcctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggttaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [291] g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)|c159>g|c164>a,A55>D(- - -)|t174>g|g188>t,G63>V(- - -)|g225>a|c248>t,S83>F(- - -)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)| g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)| c159>g|c164>a,A55>D(- - -)| t174>g|g188>t,G63>V(- - -)| g225>a|c248>t,S83>F(- - -)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)| 320 225 213 12 3 9 1 3 1 0 1 1 0 0 2 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 2 0 0 0 0 0 0 1 0 0 0 51 51 49 2 1 1 0 0 0 0 0 1 0 0 0 1 0 0 30 24 22 2 1 1 0 0 0 0 0 0 0 0 0 0 1 1 117 114 110 4 1 3 0 1 1 0 1 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct acattcgcctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtgtcagat attagtgggagtggtgttagcaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtggcggcagttgctttaactcc AKDGARGGGSCFNS IGHV3-23 IGHD5-24 IGHJ4 acattcgcctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtgtcagat attagtgggagtggtgttagcaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtggcggcagttgctttaactcc IGG2 12 213 5 7 4 9 1 3 2 1 4 5 JY8QFUQ01BJ4VI 16354 productive Homsap IGHV3-7*01 F 905 90.83 198/218 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-2*01 F 2 8 8 10 8.8.10 [1.17.38.11] CTRSSNSAYDYW in-frame + cctctggattcacgtttagtaacagttggatgggctgggcccgccaggctccagggaaggggctggagtgggtggccagcacaaaccaagatgcaagtgagaaaaagtatgtggactctgtgaggggccgattcaccatctcaagagacaacgccaagaactcactgtatttacaaatgaacagcctaagagccgaggacacggctttatatttctgtacgaggtccagtaacagcgcttatgactattggggccagggaactctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtcccagggcttnttcccccga NA IGA1 100 100 [279] c87>g|g107>a,S36>N(- - -)|t109>a,Y37>S(+ - -)|a110>g,Y37>S(+ - -)|a118>g,S40>G(+ + -)|t125>c,V42>A(+ - +)|a164>g,N55>S(- - -)|t167>c,I56>T(- - -)|g171>c,K57>N(+ - -)|g185>c,G62>A(- + -)|t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|a215>g,K72>R(+ + +)|c237>a|c265>t|g267>a|g282>a|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|a308>t,Y103>F(- + -)|g313>a,A105>T(- - -)| c87>g|g107>a,S36>N(- - -)|t109>a,Y37>S(+ - -)|a110>g,Y37>S(+ - -)| a118>g,S40>G(+ + -)|t125>c,V42>A(+ - +)|a164>g,N55>S(- - -)| t167>c,I56>T(- - -)|g171>c,K57>N(+ - -)|g185>c,G62>A(- + -)| t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|a215>g,K72>R(+ + +)|c237>a|c265>t|g267>a|g282>a|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|a308>t,Y103>F(- + -)| g313>a,A105>T(- - -)|a318>g|g319>t|a320>c| 317 (320) 223 (226) 202 21 (24) 5 (8) 16 4 (5) 5 1 2 0 (1) 1 1 2 2 2 1 (2) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 0 0 0 0 0 1 0 1 0 0 51 51 48 3 0 3 2 0 0 1 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 0 0 1 0 0 0 0 2 0 0 0 117 114 104 10 4 6 1 3 1 0 0 1 1 1 0 1 1 0 5 (8) 5 4 1 (4) 0 (3) 1 0 (1) 1 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacgtttagtaacagttgg atgggctgggcccgccaggctccagggaaggggctggagtgggtggccagc acaaaccaagatgcaagtgagaaa aagtatgtggactctgtgaggggccgattcaccatctcaagagacaacgccaagaactcactgtatttacaaatgaacagcctaagagccgaggacacggctttatatttctgt acgaggtccagtaacagcgcttatgactat TRSSNSAYDY IGHV3-7 IGHD2-2 IGHJ4 ggattcacgtttagtaacagttgg atgggctgggcccgccaggctccagggaaggggctggagtgggtggccagc acaaaccaagatgcaagtgagaaa aagtatgtggactctgtgaggggccgattcaccatctcaagagacaacgccaagaactcactgtatttacaaatgaacagcctaagagccgaggacacggctttatatttctgt acgaggtccagtaacagcgcttatgactat IGA1 20 213 11 9 5 11 6 9 4 1 9 6 JY8QFUQ01BJN3B 11377 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 868 89.35 193/216 nt 88.43 191/216 nt Homsap IGHJ1*01 F 215 90.38 47/52 nt Homsap IGHD5-12*01 F 3 8 7 14 8.7.14 [2.17.38.11] CASGGYSGSGEYFLRW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'T' (1 nucleotides at position 14 to 14 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 102 of V-REGION: 'C' (1 nucleotides at position 211 to 211 in the user submitted sequence), (cause frameshift); gcctctgacttaaTcggtcagtgacaattacatgagttgggtccgccaggctccagggaaggggctggagtgggtctcaattatttatagcggaggtcgcacatactacgcagagtccgtgaagggccgattcgccgtctccagagacggttcccagaacacactgtatcttcaaatgaacagcctgaggaccgaagacacggccgtgtaCtttttgtgcgagtggtggttattcgggctcgggtgaatatttcctacgctggggccagggcaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [291] g80>a,G27>D(- - -)|g81>c,G27>D(- - -)|c84>a,F28>L(+ - -)|c87>g|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c111>t|c120>t|g163>a,V55>I(+ - +)|t177>a|a190>c,S64>R(- - -)|c207>g,D69>E(+ - +)|a229>g,T77>A(- - -)|a232>g,I78>V(+ - +)|a244>g,N82>G(- - -)|a245>g,N82>G(- - -)|a250>c,K84>Q(+ - -)|g258>a|a285>g|g286>a,A96>T(- - -)|g291>a|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g80>a,G27>D(- - -)|g81>c,G27>D(- - -)|c84>a,F28>L(+ - -)|c87>g|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c111>t| c120>t|g163>a,V55>I(+ - +)| t177>a|a190>c,S64>R(- - -)| c207>g,D69>E(+ - +)|a229>g,T77>A(- - -)|a232>g,I78>V(+ - +)|a244>g,N82>G(- - -)|a245>g,N82>G(- - -)|a250>c,K84>Q(+ - -)|g258>a|a285>g|g286>a,A96>T(- - -)|g291>a|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| a318>t,R106>S(- - -)|a320>g| 317 (320) 221 (224) 198 (199) 23 (25) 7 (8) 16 (17) 6 (7) 6 3 0 2 1 1 (2) 1 1 2 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 2 5 1 2 1 0 0 1 0 0 1 1 0 0 51 51 49 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 30 21 19 2 1 1 0 0 0 0 1 0 0 1 0 0 0 0 117 114 102 12 3 9 5 3 1 0 1 0 1 0 0 1 0 0 5 (8) 5 5 (6) 0 (2) 0 (1) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct gacttaacggtcagtgacaattac atgagttgggtccgccaggctccagggaaggggctggagtgggtctcaatt atttatagcggaggtcgcaca tactacgcagagtccgtgaagggccgattcgccgtctccagagacggttcccagaacacactgtatcttcaaatgaacagcctgaggaccgaagacacggccgtgtatttttgt gcgagtggtggttattcgggctcgggtgaatatttcctacgc ASGGYSGSGEYFLR IGHV3-53 IGHD5-12 IGHJ1 gacttaacggtcagtgacaattac atgagttgggtccgccaggctccagggaaggggctggagtgggtctcaatt atttatagcggaggtcgcaca tactacgcagagtccgtgaagggccgattcgccgtctccagagacggttcccagaacacactgtatcttcaaatgaacagcctgaggaccgaagacacggccgtgtatttttgt gcgagtggtggttattcgggctcgggtgaatatttcctacgc IGA1 23 210 15 8 9 13 6 10 4 3 10 6 JY8QFUQ01BJPU6 10964 productive (see comment) Homsap IGHV3-11*01 F (see comment) 770 83.94 183/218 nt 83.49 182/218 nt Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD2-15*01 F 2 8 8 15 8.8.15 [1.17.38.11] CARVRCSGNTCGAFDLW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (83,94% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR2-IMGT at codon 45 of V-REGION: 'G' (1 nucleotides at position 49 to 49 in the user submitted sequence), (cause frameshift); cctctggattcagcttaagtgactactacatgacctgggtccgccaggGccccagggaagggactggagtggctcgcctacattagtcgaactgatgattccgtatattccgcagagtctgtggtgggccgattcaccgtctccagggacaacgtccaaaactcactgtttttgcagatgattggcctgagagacgaggacacggccgtatattactgtgcgagagtccgctgtagtggtaatacctgcggggcttttgatttgtggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [295] c86>g,T29>S(+ - +)|c90>a,F30>L(+ - -)|g119>c,S40>T(+ - +)|a124>g,I42>V(+ - +)|t135>c|g147>a|g157>c,V53>L(+ - +)|t159>c,V53>L(+ - +)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)|a172>c,S58>R(- - -)|t174>a,S58>R(- - -)|g176>c,S59>T(+ - +)|g185>a,G62>D(- - -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a190>t,T64>S(+ - +)|a193>g,I65>V(+ - +)|c198>t|a200>c,Y67>S(+ - -)|c207>g,D69>E(+ - +)|a214>g,K72>V(- - -)|a215>t,K72>V(- - -)|a232>g,I78>V(+ - +)|c248>t,A83>V(+ - +)|a250>c,K84>Q(+ - -)|g252>a,K84>Q(+ - -)|a263>t,Y88>F(- + -)|c265>t|a270>g|a275>t,N92>I(- - -)|c276>t,N92>I(- - -)|a277>g,S93>G(+ + -)|c287>a,A96>D(- - -)|g303>a| c86>g,T29>S(+ - +)|c90>a,F30>L(+ - -)| g119>c,S40>T(+ - +)|a124>g,I42>V(+ - +)|t135>c|g147>a|g157>c,V53>L(+ - +)|t159>c,V53>L(+ - +)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)| a172>c,S58>R(- - -)|t174>a,S58>R(- - -)|g176>c,S59>T(+ - +)|g185>a,G62>D(- - -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a190>t,T64>S(+ - +)|a193>g,I65>V(+ - +)| c198>t|a200>c,Y67>S(+ - -)|c207>g,D69>E(+ - +)|a214>g,K72>V(- - -)|a215>t,K72>V(- - -)|a232>g,I78>V(+ - +)|c248>t,A83>V(+ - +)|a250>c,K84>Q(+ - -)|g252>a,K84>Q(+ - -)|a263>t,Y88>F(- + -)|c265>t|a270>g|a275>t,N92>I(- - -)|c276>t,N92>I(- - -)|a277>g,S93>G(+ + -)|c287>a,A96>D(- - -)|g303>a| a320>t| 319 (320) 225 (226) 190 35 (36) 6 (7) 29 7 5 4 2 4 2 4 (5) 1 3 2 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 1 0 0 0 1 0 0 51 51 43 8 2 6 1 1 0 2 1 0 0 0 2 0 0 1 30 24 16 8 0 8 2 2 0 0 1 0 1 1 1 0 0 0 117 114 97 17 4 13 4 2 4 0 2 1 3 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcagcttaagtgactactac atgacctgggtccgccaggccccagggaagggactggagtggctcgcctac attagtcgaactgatgattccgta tattccgcagagtctgtggtgggccgattcaccgtctccagggacaacgtccaaaactcactgtttttgcagatgattggcctgagagacgaggacacggccgtatattactgt gcgagagtccgctgtagtggtaatacctgcggggcttttgatttg ARVRCSGNTCGAFDL IGHV3-11 IGHD2-15 IGHJ3 ggattcagcttaagtgactactac atgacctgggtccgccaggccccagggaagggactggagtggctcgcctac attagtcgaactgatgattccgta tattccgcagagtctgtggtgggccgattcaccgtctccagggacaacgtccaaaactcactgtttttgcagatgattggcctgagagacgaggacacggccgtatattactgt gcgagagtccgctgtagtggtaatacctgcggggcttttgatttg IGA2 35 213 18 17 9 16 9 19 6 0 19 10 JY8QFUQ01BJT4G 22110 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 946 92.69 203/219 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-2*01 F 3 8 8 11 8.8.11 [2.17.38.11] CARFGPLTRPDYW in-frame - gcctctggattcacctttaccaactatggcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtggtgaaaccacataccacgcagaatccgtgcagggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgtgcgagattcggaccacttactcgacctgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [284] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)|c164>g,A55>G(- + -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>c,S64>T(+ - +)|t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a214>c,K72>Q(+ - -)|t246>c|c257>a,T86>N(- + -)|g258>t,T86>N(- + -)|c279>t|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)| c164>g,A55>G(- + -)| g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>c,S64>T(+ - +)| t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a214>c,K72>Q(+ - -)|t246>c|c257>a,T86>N(- + -)|g258>t,T86>N(- + -)|c279>t|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| a317>g,K106>R(+ + +)|g319>t|a320>t| 316 (320) 223 (227) 207 (208) 16 (19) 2 (4) 14 (15) 0 (1) 3 1 2 1 2 1 (2) 1 2 2 1 (2) 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 21 3 0 3 0 1 0 0 0 0 0 1 1 0 0 0 117 114 105 9 2 7 0 1 1 2 1 2 1 0 0 0 1 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgaaaccaca taccacgcagaatccgtgcagggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgt gcgagattcggaccacttactcgacctgactac ARFGPLTRPDY IGHV3-23 IGHD2-2 IGHJ4 ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgaaaccaca taccacgcagaatccgtgcagggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgt gcgagattcggaccacttactcgacctgactac IGG1 16 213 6 10 4 11 2 5 2 0 8 6 JY8QFUQ01BJUHM 4969 productive Homsap IGHV3-11*01 F 945 93.09 202/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD5-18*01 F 3 8 8 18 8.8.18 [1.17.38.11] CVRRVWGYSYGSVSYYFDYW in-frame + ctctggattcaccttcagtgactactacatgaactggatccgccaggctccagggaaggggctggagtggatttcatacattagtgggagtgcgactaccatatcctacgcagactttgcgaagggccgattcaccatctccagggacaacgccaagaactcggtgtatctgcaaatgaacagcctgagagccgaggacacggccacgtattattgtgtgaggcgggtctggggatacagttatggttccgtttcgtattacttcgactactggggccagggaatcccggtcaccgtctcctcagcatccccggaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [303] g119>a,S40>N(- - -)|g157>a,V53>I(+ - +)|a172>g,S58>G(+ + -)|t174>g,S58>G(+ + -)|g185>c,G62>A(- + -)|t186>g,G62>A(- + -)|g188>c,S63>T(+ - +)|a197>c,Y66>S(+ - -)|c209>t,S70>F(- - -)|t212>c,V71>A(+ - +)|a258>g|c259>g,L87>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|c309>t|c314>t,A105>V(+ - +)| g119>a,S40>N(- - -)|g157>a,V53>I(+ - +)| a172>g,S58>G(+ + -)|t174>g,S58>G(+ + -)|g185>c,G62>A(- + -)|t186>g,G62>A(- + -)|g188>c,S63>T(+ - +)| a197>c,Y66>S(+ - -)|c209>t,S70>F(- - -)|t212>c,V71>A(+ - +)|a258>g|c259>g,L87>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|c309>t| c314>t,A105>V(+ - +)|a318>g|g319>c|a320>g| 317 (320) 222 (225) 206 16 (19) 2 (5) 14 2 (4) 3 3 2 1 0 0 0 2 (3) 1 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 2 0 0 0 0 0 0 0 0 0 0 30 24 19 5 0 5 1 0 0 0 0 0 0 0 2 0 0 2 117 114 106 8 2 6 1 1 2 2 1 0 0 0 0 1 0 0 5 (8) 5 4 1 (4) 0 (3) 1 0 (2) 0 1 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgactactac atgaactggatccgccaggctccagggaaggggctggagtggatttcatac attagtgggagtgcgactaccata tcctacgcagactttgcgaagggccgattcaccatctccagggacaacgccaagaactcggtgtatctgcaaatgaacagcctgagagccgaggacacggccacgtattattgt gtgaggcgggtctggggatacagttatggttccgtttcgtattacttcgactac VRRVWGYSYGSVSYYFDY IGHV3-11 IGHD5-18 IGHJ4 ggattcaccttcagtgactactac atgaactggatccgccaggctccagggaaggggctggagtggatttcatac attagtgggagtgcgactaccata tcctacgcagactttgcgaagggccgattcaccatctccagggacaacgccaagaactcggtgtatctgcaaatgaacagcctgagagccgaggacacggccacgtattattgt gtgaggcgggtctggggatacagttatggttccgtttcgtattacttcgactac IGA1 15 213 9 6 5 8 4 7 2 0 8 5 JY8QFUQ01BJURD 18891 productive Homsap IGHV4-61*02 F 983 94.12 208/221 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-6*01 F 1 10 7 15 10.7.15 [1.17.38.11] CARNPSYSNSHQHCDYW in-frame + tctctggtgcctccatcaacagtggtagttactactggagttgggttcggcagcccgccgggaagggactggagtggattgggcgtatctacaccagtgggagcaccaactacaacccctccctcaagagtcgagtcgccatatcaatggacacgtccaagaaccagttctccctgaagctgaactctgtgaccgccgcagacacggccgtctatttttgtgcgagaaatccctcttatagcaattcgcaccagcattgtgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] g83>c,G28>A(- + -)|g92>a,S31>N(- - -)|c120>t|a124>g,I42>V(+ - +)|c126>t,I42>V(+ - +)|t171>c|a229>g,T77>A(- - -)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|g275>a,S92>N(- - -)|g303>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g83>c,G28>A(- + -)|g92>a,S31>N(- - -)| c120>t|a124>g,I42>V(+ - +)|c126>t,I42>V(+ - +)| t171>c| a229>g,T77>A(- - -)|g238>a,V80>M(+ - -)|a240>g,V80>M(+ - -)|g275>a,S92>N(- - -)|g303>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g319>a| 318 (320) 227 (229) 214 (215) 13 (14) 3 (4) 10 3 3 (4) 3 1 0 0 1 0 2 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 51 51 48 3 1 2 1 0 2 0 0 0 0 0 0 0 0 0 30 21 20 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 107 7 1 6 2 2 1 0 0 0 1 0 1 0 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgcctccatcaacagtggtagttactac tggagttgggttcggcagcccgccgggaagggactggagtggattgggcgt atctacaccagtgggagcacc aactacaacccctccctcaagagtcgagtcgccatatcaatggacacgtccaagaaccagttctccctgaagctgaactctgtgaccgccgcagacacggccgtctatttttgt gcgagaaatccctcttatagcaattcgcaccagcattgtgactac ARNPSYSNSHQHCDY IGHV4-61 IGHD6-6 IGHJ4 ggtgcctccatcaacagtggtagttactac tggagttgggttcggcagcccgccgggaagggactggagtggattgggcgt atctacaccagtgggagcacc aactacaacccctccctcaagagtcgagtcgccatatcaatggacacgtccaagaaccagttctccctgaagctgaactctgtgaccgccgcagacacggccgtctatttttgt gcgagaaatccctcttatagcaattcgcaccagcattgtgactac IGA1 13 216 10 3 6 8 4 5 2 1 8 2 JY8QFUQ01BJV9I 4046 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 891 90.32 196/217 nt 89.86 195/217 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-10*02 F 3 8 8 12 8.8.12 [1.17.38.11] CVKDISRSGCFEYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 64 of V-REGION: 'C' (1 nucleotides at position 99 to 99 in the user submitted sequence), (cause frameshift); ctctggattcaccttcagtagatactggatgcactgggtccgccaggctccagggaaggggctggagtgggtcgcacgtactaatgaagatgggagtaCtaaaaagctacgcggactccgtgaagggccgattcaccatcttcagagacaacaccaagaacacactatatctgcaaatgaacagcctgacagccgaggacacggccagatattactgtgtgaaagacatttccaggtcaggctgctttgagtattggggtcagggaaccctgatcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatggaacgtggtcatcgcctgcctggtccagggcttcttcccccaag NA IGA1 100 89 [285, 284] c108>a,S36>R(- - -)|a132>g|t152>a,V51>E(- + -)|t160>g,S54>A(- + -)|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)|c192>a,S64>I(- - -)|c194>a,T65>K(- - -)|c236>t,S79>F(- - -)|g247>a,A83>T(- - -)|g258>a|g261>a|t279>c|g284>c,R95>T(- - -)|t300>c|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)|g303>a,V101>R(- - -)| c108>a,S36>R(- - -)| a132>g|t152>a,V51>E(- + -)|t160>g,S54>A(- + -)| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)|c192>a,S64>I(- - -)|c194>a,T65>K(- - -)| c236>t,S79>F(- - -)|g247>a,A83>T(- - -)|g258>a|g261>a|t279>c|g284>c,R95>T(- - -)|t300>c|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)|g303>a,V101>R(- - -)| c314>t,A105>V(+ - +)|a315>g,A105>V(+ - +)|g317>a,R106>K(+ + +)| 320 225 201 24 5 19 3 7 2 3 0 3 0 2 1 0 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 48 3 1 2 1 0 0 0 0 0 0 1 0 0 0 1 30 24 17 7 0 7 1 1 0 1 0 2 0 1 0 0 1 0 117 114 104 10 4 6 0 5 1 2 0 0 0 0 1 0 0 1 8 8 5 3 0 3 1 1 1 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagatactgg atgcactgggtccgccaggctccagggaaggggctggagtgggtcgcacgt actaatgaagatgggagtataaaa agctacgcggactccgtgaagggccgattcaccatcttcagagacaacaccaagaacacactatatctgcaaatgaacagcctgacagccgaggacacggccagatattactgt gtgaaagacatttccaggtcaggctgctttgagtat VKDISRSGCFEY IGHV3-74 IGHD3-10 IGHJ4 ggattcaccttcagtagatactgg atgcactgggtccgccaggctccagggaaggggctggagtgggtcgcacgt actaatgaagatgggagtataaaa agctacgcggactccgtgaagggccgattcaccatcttcagagacaacaccaagaacacactatatctgcaaatgaacagcctgacagccgaggacacggccagatattactgt gtgaaagacatttccaggtcaggctgctttgagtat IGA1 21 213 12 9 7 12 5 9 5 0 8 8 JY8QFUQ01BJVZ3 19229 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 871 88.29 196/222 nt 87.84 195/222 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [3.17.38.11] CVRAGFGELSGFDCW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'C' (1 nucleotides at position 17 to 17 in the user submitted sequence), (cause frameshift); aggcctcctggattcaCcctttggttattatggcatggactgggtccgccaactccgggggagggggctggagtgggtctctggtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [294] g70>a,A24>R(- - -)|c71>g,A24>R(- - -)|a72>g,A24>R(- - -)|g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>P(+ - -)|a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a118>g,S40>D(- - -)|g119>a,S40>D(- - -)|g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|t135>c,A45>L(+ - +)|c137>g,P46>R(- - -)|a138>g,P46>R(- - -)|a143>g,K48>R(+ + +)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| g70>a,A24>R(- - -)|c71>g,A24>R(- - -)|a72>g,A24>R(- - -)|g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>P(+ - -)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a118>g,S40>D(- - -)|g119>a,S40>D(- - -)|g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|t135>c,A45>L(+ - +)|c137>g,P46>R(- - -)|a138>g,P46>R(- - -)|a143>g,K48>R(+ + +)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 229 (230) 202 27 (28) 2 (3) 25 5 5 3 3 1 (2) 0 2 0 4 3 1 0 78 9 3 6 0 6 1 1 1 1 0 0 0 0 1 1 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 51 43 8 0 8 3 1 1 1 0 0 0 0 1 1 0 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| aggcctcct ggattcacctttggttattatggc atggactgggtccgccaactccgggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atggactgggtccgccaactccgggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 20 213 11 9 5 11 6 9 2 0 14 4 JY8QFUQ01BJWBZ 3378 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 922 92.13 199/216 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD4-23*01 ORF 2 8 8 14 8.8.14 [1.17.38.11] CARAYSGPGPYYFDYW in-frame + tccggattcaacttcaattactttagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattagtagtgatggtacttacatatactacgcagactcagtgaagggccgattcgccatctccagagacaacgccaaagactcactgtatctacaaatgaacatcctgagagccgaggacgcggctgtttattactgtgcgagagcctacagtggccctgggccttactactttgactattggggccagggaatcctggtcaccgtctcctccgcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccaggggcttcttccccca NA IGA2 100 100 [289] t78>c|c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a110>t,Y37>F(- + -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|a229>g,T77>A(- - -)|g252>a|a253>g,N85>D(+ + -)|g267>a|g278>t,S93>I(- - -)|a295>g,T99>A(- - -)|g303>t| t78>c| c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a110>t,Y37>F(- + -)| a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)| a229>g,T77>A(- - -)|g252>a|a253>g,N85>D(+ + -)|g267>a|g278>t,S93>I(- - -)|a295>g,T99>A(- - -)|g303>t| a320>c| 319 (320) 223 (224) 206 17 (18) 4 (5) 13 5 5 0 1 0 (1) 1 2 0 1 0 2 0 78 3 2 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 2 0 0 0 1 2 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 2 1 0 0 0 0 0 0 1 0 0 0 117 114 107 7 3 4 3 2 0 0 0 0 0 0 0 0 2 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tcc ggattcaacttcaattactttagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtgatggtacttacata tactacgcagactcagtgaagggccgattcgccatctccagagacaacgccaaagactcactgtatctacaaatgaacatcctgagagccgaggacgcggctgtttattactgt gcgagagcctacagtggccctgggccttactactttgactat ARAYSGPGPYYFDY IGHV3-21 IGHD4-23 IGHJ4 ggattcaacttcaattactttagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtgatggtacttacata tactacgcagactcagtgaagggccgattcgccatctccagagacaacgccaaagactcactgtatctacaaatgaacatcctgagagccgaggacgcggctgtttattactgt gcgagagcctacagtggccctgggccttactactttgactat IGA2 16 213 10 6 5 9 5 7 3 0 4 9 JY8QFUQ01BJYH6 14898 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 841 87.96 190/216 nt 87.5 189/216 nt Homsap IGHJ6*02 F 247 88.71 55/62 nt Homsap IGHD2-8*01 F 1 8 8 19 8.8.19 [1.16.38.11] CAKVFQVSGEHHFYYYGMNAW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 57 in the user submitted sequence), (cause frameshift) ctctggattcatctttagtaattatgccatgagttgggtccgccaggccccagggaggggctggagtgggtctcaactatcagtgccaatggagacaacacatactacgcggactccgtgaagggccgattcaccatctccagagacaattccaagagcacagtgtatatgcaaatgaacagcctgaaagccgaggagacggccgtctatcattgtgcgaaagtcttccaggtgtctggcgaacatcacttctactactatggtatgaatgcctggggccaagggaccacggtcaccgtctccccagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [304] c86>t,T29>I(- - -)|c105>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|c120>t|t135>c|g163>a,A55>T(- - -)|t168>c|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g176>a,S59>N(- - -)|t186>a|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|g191>a,S64>N(- - -)|a204>g|g225>a|a254>g,N85>S(- - -)|g258>a|c259>g,L87>V(+ - +)|c265>a,L89>M(+ + -)|g284>a,R95>K(+ + +)|c294>g,D98>E(+ - +)|a303>c|t307>c,Y103>H(+ - -)|c309>t,Y103>H(+ - -)| c86>t,T29>I(- - -)|c105>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| c120>t|t135>c|g163>a,A55>T(- - -)| t168>c|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g176>a,S59>N(- - -)|t186>a|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|g191>a,S64>N(- - -)| a204>g|g225>a|a254>g,N85>S(- - -)|g258>a|c259>g,L87>V(+ - +)|c265>a,L89>M(+ + -)|g284>a,R95>K(+ + +)|c294>g,D98>E(+ - +)|a303>c|t307>c,Y103>H(+ - -)|c309>t,Y103>H(+ - -)| a320>t| 319 (320) 223 (224) 197 26 (27) 9 (10) 17 2 8 5 5 1 1 0 (1) 1 1 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 3 0 0 0 0 0 0 0 0 0 51 50 47 3 2 1 0 1 1 1 0 0 0 0 0 0 0 0 30 24 16 8 2 6 0 3 0 3 0 0 0 1 1 0 0 0 117 114 103 11 4 7 2 3 1 1 1 1 0 0 0 2 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatctttagtaattatgcc atgagttgggtccgccaggccccagggaggggctggagtgggtctcaact atcagtgccaatggagacaacaca tactacgcggactccgtgaagggccgattcaccatctccagagacaattccaagagcacagtgtatatgcaaatgaacagcctgaaagccgaggagacggccgtctatcattgt gcgaaagtcttccaggtgtctggcgaacatcacttctactactatggtatgaatgcc AKVFQVSGEHHFYYYGMNA IGHV3-23 IGHD2-8 IGHJ6 ggattcatctttagtaattatgcc atgagttgggtccgccaggccccagggaggggctggagtgggtctcaact atcagtgccaatggagacaacaca tactacgcggactccgtgaagggccgattcaccatctccagagacaattccaagagcacagtgtatatgcaaatgaacagcctgaaagccgaggagacggccgtctatcattgt gcgaaagtcttccaggtgtctggcgaacatcacttctactactatggtatgaatgcc IGA1 26 212 20 6 13 17 7 9 6 3 8 9 JY8QFUQ01BKBQY 5670 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD5-18*01 F 1 8 8 11 8.8.11 [1.17.38.11] CVRHTAMERGDYW in-frame - ttctggatatgactttatcaactactggatcggctgggtgcgccagatgcccgggagaggcctggaatggatgggaatcatctttcctgatgactctgatgccagatatagtccgtccttccagggccacgtcaccatctcagccgacaagtccacaagcaccgcctacctggagtggagcagcctgaaggcctcggacaccgccatctactactgtgtgagacatacagctatggagaggggtgactactggggcccgggaaccctggtcaccgtcacctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcc NA IGA1 100 100 [281] c84>t|a85>g,S29>D(- - -)|g86>a,S29>D(- - -)|c104>t,T35>I(- - -)|g107>a,S36>N(- - -)|a143>g,K48>R(+ + +)|g153>a|g162>a|a170>t,Y57>F(- + -)|g176>a,G59>D(- - -)|a193>g,T65>A(- - -)|c201>t|c204>t|a216>g|g225>c,Q75>H(- + -)|t251>c,I84>T(- - -)|c252>a,I84>T(- - -)|c268>g,Q90>E(+ + -)|g303>c,M101>I(+ + -)|t306>c|c314>t,A105>V(+ - +)| c84>t|a85>g,S29>D(- - -)|g86>a,S29>D(- - -)|c104>t,T35>I(- - -)|g107>a,S36>N(- - -)| a143>g,K48>R(+ + +)|g153>a|g162>a| a170>t,Y57>F(- + -)|g176>a,G59>D(- - -)|a193>g,T65>A(- - -)| c201>t|c204>t|a216>g|g225>c,Q75>H(- + -)|t251>c,I84>T(- - -)|c252>a,I84>T(- - -)|c268>g,Q90>E(+ + -)|g303>c,M101>I(+ + -)|t306>c| c314>t,A105>V(+ - +)| 320 225 204 21 7 14 4 5 5 2 0 1 1 0 2 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 1 2 2 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 1 2 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 1 0 0 0 0 1 0 0 0 0 0 117 114 105 9 4 5 1 0 2 2 0 1 0 0 2 1 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ttct ggatatgactttatcaactactgg atcggctgggtgcgccagatgcccgggagaggcctggaatggatgggaatc atctttcctgatgactctgatgcc agatatagtccgtccttccagggccacgtcaccatctcagccgacaagtccacaagcaccgcctacctggagtggagcagcctgaaggcctcggacaccgccatctactactgt gtgagacatacagctatggagaggggtgactac VRHTAMERGDY IGHV5-51 IGHD5-18 IGHJ4 ggatatgactttatcaactactgg atcggctgggtgcgccagatgcccgggagaggcctggaatggatgggaatc atctttcctgatgactctgatgcc agatatagtccgtccttccagggccacgtcaccatctcagccgacaagtccacaagcaccgcctacctggagtggagcagcctgaaggcctcggacaccgccatctactactgt gtgagacatacagctatggagaggggtgactac IGA1 20 213 15 5 9 13 6 7 6 1 6 7 JY8QFUQ01BKF2J 10870 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 712 80.82 177/219 nt 80.37 176/219 nt Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD2-21*02 F 2 8 8 15 8.8.15 [2.17.38.11] CARYRVGDSLYYGLDGW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (80,82% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR2-IMGT at codon 59 of V-REGION: 'ACT' (3 nucleotides at position 91 to 93 in the user submitted sequence), (do not cause frameshift); gcctctggattcattttagaaaattttgccatgagttggctccgccaggcaccagggaaggggctggaatgggtctcgactatcagcagcACTagtggtgacacggcatattactcagactccgtgaggggccgcttttccatctccagagacaactccaagagcactctgttcttgcagatgaacagcctgagtgccgaagacacggccatttactactgtgcgagataccgtgtgggtgacagtctctactacggtttggacggctggggccaagggaccacggtcaccgtctcgtcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [298] c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|t90>a,F30>L(+ - -)|a103>g,S35>E(- - -)|g104>a,S35>E(- - -)|c105>a,S35>E(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a110>t,Y37>F(- + -)|c120>t|g124>c,V42>L(+ - +)|t135>a|g153>a|a162>g|g163>a,A55>T(- - -)|t168>c|t171>c|g172>a,G58>S(+ + -)|t174>c,G58>S(+ + -)|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|g191>c,S64>T(+ - +)|c192>g,S64>T(+ - +)|a193>g,T65>A(- - -)|c198>t|g202>t,A68>S(- + -)|a215>g,K72>R(+ + +)|g225>c|c228>t|a229>t,T77>S(+ - +)|t246>c|a254>g,N85>S(- - -)|g258>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|a270>g|a285>t,R95>S(- - -)|g291>a|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)|t306>c| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|t90>a,F30>L(+ - -)|a103>g,S35>E(- - -)|g104>a,S35>E(- - -)|c105>a,S35>E(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a110>t,Y37>F(- + -)| c120>t|g124>c,V42>L(+ - +)|t135>a|g153>a|a162>g|g163>a,A55>T(- - -)| t168>c|t171>c|g172>a,G58>S(+ + -)|t174>c,G58>S(+ + -)|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|g191>c,S64>T(+ - +)|c192>g,S64>T(+ - +)|a193>g,T65>A(- - -)| c198>t|g202>t,A68>S(- + -)|a215>g,K72>R(+ + +)|g225>c|c228>t|a229>t,T77>S(+ - +)|t246>c|a254>g,N85>S(- - -)|g258>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|a270>g|a285>t,R95>S(- - -)|g291>a|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)|t306>c| a317>g,K106>R(+ + +)|g319>t| 316 (320) 223 (227) 181 (183) 42 (44) 15 (16) 27 (28) 6 (7) 8 7 7 0 1 5 2 3 1 2 (3) 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 15 9 0 9 1 2 3 0 0 1 1 1 0 0 0 0 51 51 45 6 4 2 1 2 1 0 0 0 0 1 1 0 0 0 30 24 15 9 2 7 1 2 0 4 0 0 0 0 1 1 0 0 117 114 96 18 9 9 3 2 3 3 0 0 4 0 1 0 2 0 4 (8) 4 4 (6) 0 (2) 0 (1) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcattttagaaaattttgcc atgagttggctccgccaggcaccagggaaggggctggaatgggtctcgact atcagcagcagtggtgacacggca tattactcagactccgtgaggggccgcttttccatctccagagacaactccaagagcactctgttcttgcagatgaacagcctgagtgccgaagacacggccatttactactgt gcgagataccgtgtgggtgacagtctctactacggtttggacggc ARYRVGDSLYYGLDG IGHV3-23 IGHD2-21 IGHJ6 ggattcattttagaaaattttgcc atgagttggctccgccaggcaccagggaaggggctggaatgggtctcgact atcagcagcagtggtgacacggca tattactcagactccgtgaggggccgcttttccatctccagagacaactccaagagcactctgttcttgcagatgaacagcctgagtgccgaagacacggccatttactactgt gcgagataccgtgtgggtgacagtctctactacggtttggacggc IGA1 42 213 28 14 15 22 13 20 13 2 11 16 JY8QFUQ01BKJ53 14039 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 945 93.09 202/217 nt 92.63 201/217 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 3 8 7 12 8.7.12 [1.17.38.11] CARDTGQWKDFDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 58 of V-REGION: 1 nucleotides (from position 88 in the user submitted sequence), (cause frameshift) cctctggattcatcttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgtagtaatacggggggactgacacagcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgtgcaagagatacggggcagtggaaggactttgactactggggccagggaaccctggtcaccgtctccttagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctgtccagggcttcttccccca NA IGA1 100 100 [284] c86>t,T29>I(- - -)|c159>g|t167>g,I56>S(- - -)|t174>c|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| c86>t,T29>I(- - -)| c159>g| t167>g,I56>S(- - -)|t174>c|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| 320 225 210 15 4 11 3 1 1 1 1 0 0 0 2 3 1 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 30 23 16 7 1 6 2 1 0 1 0 0 0 0 1 0 0 2 117 114 108 6 2 4 1 0 0 0 1 0 0 0 1 2 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgt agtaatacggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac ARDTGQWKDFDY IGHV3-74 IGHD6-19 IGHJ4 ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgt agtaatacggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac IGA1 15 212 6 9 2 8 4 7 3 1 4 7 JY8QFUQ01BKRE0 8264 productive Homsap IGHV1-46*01 F, or Homsap IGHV1-46*03 F 984 95.79 205/214 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD3-22*01 F 2 8 8 14 8.8.14 [0.17.38.11] CASAPSDSSGYYFSYW in-frame + tggatacaccttcaccagttactatatgcactgggtccgacaggcccctggacaagggcttgagtggatgggaatgatcaaccctagtggcggaagcacaatctacgcacagaacttccagggcagagttgccatgaccagggacacgtccacgagcacagtctacatggagctgagcagcctgagatctgaggacacggccgtgtattactgtgctagcgccccctctgatagtagtggatactacttttcctactggggccagggaaccctggtcaccgtctcctcagcatcccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [286] c108>t|g126>c|a165>g,I55>M(+ + -)|t186>c|t189>a|g197>t,S66>I(- - -)|g210>c,K70>N(+ - -)|c228>t|a229>g,T77>A(- - -)|g315>t| c108>t| g126>c|a165>g,I55>M(+ + -)| t186>c|t189>a| g197>t,S66>I(- - -)|g210>c,K70>N(+ - -)|c228>t|a229>g,T77>A(- - -)| g315>t|a318>c,R106>S(- - -)|a320>c| 317 (320) 219 (222) 209 (210) 10 (12) 6 (7) 4 (5) 2 0 2 1 0 (2) 0 0 1 2 0 2 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 1 0 0 0 0 0 0 0 1 0 0 0 30 24 22 2 2 0 0 0 0 1 0 0 0 1 0 0 0 0 117 114 110 4 1 3 1 0 1 0 0 0 0 0 1 0 1 0 5 (8) 5 4 (5) 1 (3) 1 (2) 0 (1) 0 0 0 0 0 (2) 0 0 0 0 0 1 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,162-163(FR2)|ta,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,62-63(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,164-165(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggca,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|aacc,169-172(CDR2)|agct,196-199(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcaccagttactat atgcactgggtccgacaggcccctggacaagggcttgagtggatgggaatg atcaaccctagtggcggaagcaca atctacgcacagaacttccagggcagagttgccatgaccagggacacgtccacgagcacagtctacatggagctgagcagcctgagatctgaggacacggccgtgtattactgt gctagcgccccctctgatagtagtggatactacttttcctac ASAPSDSSGYYFSY IGHV1-46 IGHD3-22 IGHJ4 ggatacaccttcaccagttactat atgcactgggtccgacaggcccctggacaagggcttgagtggatgggaatg atcaaccctagtggcggaagcaca atctacgcacagaacttccagggcagagttgccatgaccagggacacgtccacgagcacagtctacatggagctgagcagcctgagatctgaggacacggccgtgtattactgt gctagcgccccctctgatagtagtggatactacttttcctac IGA1 9 213 5 4 2 5 3 4 2 3 4 0 JY8QFUQ01BKUNY 8405 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 826 86.04 191/222 nt NA Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD3-10*02 F 2 10 7 11 10.7.11 [2.17.38.11] CVRHVRRFWFDPW in-frame - tctctaatgggctccatcagcggaagtgtttactactgggcctggatccgccagcccccagagaagggtctggagtacattggaagcatcttttatagtgggagcacttacttcaatccgtccctcaagagtcgagtcaccctatccgtagacacgtccaggaaccagttctccctgaggctgaagtctgtgaccgccgcagacacggctgtttattattgtgtgagacatgttcgacgattctggttcgacccctggggccagggaaccctggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [286] g73>t,V25>S(- - -)|t74>c,V25>S(- - -)|c75>t,V25>S(- - -)|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>a,S26>L(- - -)|g79>a,G27>M(- - -)|g80>t,G27>M(- - -)|t81>g,G27>M(- - -)|a100>g,S34>G(+ + -)|t102>a,S34>G(+ + -)|a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|g119>c,G40>A(- + -)|g140>a,G47>E(- - -)|g147>t|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|g162>a|t165>c|a170>t,Y57>F(- + -)|c195>t|a200>t,Y67>F(- + -)|c204>t|a232>c,I78>L(+ + +)|a251>g,K84>R(+ + +)|a269>g,K90>R(+ + +)|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g303>t|c309>t|c314>t,A105>V(+ - +)| g73>t,V25>S(- - -)|t74>c,V25>S(- - -)|c75>t,V25>S(- - -)|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>a,S26>L(- - -)| g79>a,G27>M(- - -)|g80>t,G27>M(- - -)|t81>g,G27>M(- - -)|a100>g,S34>G(+ + -)|t102>a,S34>G(+ + -)|a106>g,S36>V(- - -)|g107>t,S36>V(- - -)| g119>c,G40>A(- + -)|g140>a,G47>E(- - -)|g147>t|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|g162>a|t165>c| a170>t,Y57>F(- + -)|c195>t| a200>t,Y67>F(- + -)|c204>t|a232>c,I78>L(+ + +)|a251>g,K84>R(+ + +)|a269>g,K90>R(+ + +)|g275>a,S92>K(- - -)|c276>g,S92>K(- - -)|g303>t|c309>t| c314>t,A105>V(+ - +)| 320 230 198 32 7 25 4 5 6 3 1 0 2 2 2 1 5 1 78 6 0 6 0 6 0 0 2 2 0 0 0 1 0 0 1 0 36 30 23 7 0 7 2 1 0 0 0 0 0 1 0 0 2 1 51 51 44 7 3 4 0 3 0 1 0 0 0 0 2 0 1 0 30 21 19 2 1 1 0 0 1 0 0 0 1 0 0 0 0 0 117 114 105 9 3 6 2 1 2 0 1 0 1 0 0 1 1 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctcta atgggctccatcagcggaagtgtttactac tgggcctggatccgccagcccccagagaagggtctggagtacattggaagc atcttttatagtgggagcact tacttcaatccgtccctcaagagtcgagtcaccctatccgtagacacgtccaggaaccagttctccctgaggctgaagtctgtgaccgccgcagacacggctgtttattattgt gtgagacatgttcgacgattctggttcgacccc VRHVRRFWFDP IGHV4-39 IGHD3-10 IGHJ5 atgggctccatcagcggaagtgtttactac tgggcctggatccgccagcccccagagaagggtctggagtacattggaagc atcttttatagtgggagcact tacttcaatccgtccctcaagagtcgagtcaccctatccgtagacacgtccaggaaccagttctccctgaggctgaagtctgtgaccgccgcagacacggctgtttattattgt gtgagacatgttcgacgattctggttcgacccc IGA1 25 216 13 12 8 15 5 10 6 1 10 8 JY8QFUQ01BKXS7 14096 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 818 86.98 187/215 nt 86.51 186/215 nt Homsap IGHJ4*02 F (see comment) 213 93.75 45/48 nt Homsap IGHD3-10*01 F 3 8 7 16 8.7.16 [1.17.38.11] CAKESLVRGVISYYFDDW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) in CDR2-IMGT, from codon 58 of V-REGION: 3 nucleotides (from position 87 in the user submitted sequence), (do not cause frameshift) cctctggattcaactttggcatctataccatggcctgggtccgccaggctccagggaaggggctggagtgggtctcagctattcgtgatcatgatagcacatactacgcagactccgtgcagggccggtttttcatctcgagagacaatttcaataatacattgtatctgcaaatggatggcctgcgagccgacgacacggccgtctattactgtgcgaaagagtctctggttcggggagtcatctcatactactttgatgactggggccaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [294] c86>a,T29>N(- + -)|a103>g,S35>G(+ + -)|g107>t,S36>I(- - -)|g112>a,A38>T(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a169>c,S57>R(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g184>c,G62>H(+ - -)|g185>a,G62>H(+ - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|c228>t|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|c237>g|c248>t,S83>F(- - -)|g252>t,K84>N(+ - -)|c255>t|g258>a|c259>t|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|a277>g,S93>G(+ + -)|a283>c|g291>c,E97>D(+ - +)|a303>c| c86>a,T29>N(- + -)|a103>g,S35>G(+ + -)|g107>t,S36>I(- - -)|g112>a,A38>T(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)| a169>c,S57>R(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g184>c,G62>H(+ - -)|g185>a,G62>H(+ - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|c228>t|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|c237>g|c248>t,S83>F(- - -)|g252>t,K84>N(+ - -)|c255>t|g258>a|c259>t|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|a277>g,S93>G(+ + -)|a283>c|g291>c,E97>D(+ - +)|a303>c| 320 223 195 28 7 21 5 5 6 0 4 1 1 0 3 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 0 0 0 1 0 0 0 0 1 0 51 51 49 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 30 21 15 6 0 6 1 3 0 0 1 0 0 0 1 0 0 0 117 114 98 16 7 9 2 1 6 0 3 0 1 0 1 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaactttggcatctatacc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcagct attcgtgatcatgatagcaca tactacgcagactccgtgcagggccggtttttcatctcgagagacaatttcaataatacattgtatctgcaaatggatggcctgcgagccgacgacacggccgtctattactgt gcgaaagagtctctggttcggggagtcatctcatactactttgatgac AKESLVRGVISYYFDD IGHV3-23 IGHD3-10 IGHJ4 ggattcaactttggcatctatacc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcagct attcgtgatcatgatagcaca tactacgcagactccgtgcagggccggtttttcatctcgagagacaatttcaataatacattgtatctgcaaatggatggcctgcgagccgacgacacggccgtctattactgt gcgaaagagtctctggttcggggagtcatctcatactactttgatgac IGA1 28 210 16 12 11 18 5 10 7 0 11 10 JY8QFUQ01BL6V1 14637 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F (see comment) 750 83.64 179/214 nt 83.18 178/214 nt Homsap IGHJ6*02 F (see comment) 193 79.03 49/62 nt Homsap IGHD5-24*01 ORF 3 8 7 18 8.7.18 [1.17.38.11] CARKGPRRDGSLQQCDMDVW in-frame + Potentially, because of detected insertion/deletion The submitted sequence and the closest germline V-GENE allele show different CDR2-IMGT amino acid lengths (7 AA in JY8QFUQ01BL6V1 ; 8 AA in Homsap_IGHV3-21*01), and low V-REGION identity (83,64% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*03 (shorter alignment but highest percentage of identity) in FR3-IMGT at codon 93 of V-REGION: 'A' (1 nucleotides at position 179 to 179 in the user submitted sequence), (cause frameshift); ctctggattcagtttcactggttttaccgtgatctgggtccgccaggctccaaggaaggggctggaatggatctcatccgtcactactaatggtctcacgtactacgcagactcagtagagggccgattcaacatctccagggacaacgccaacaatttagtgtttctgcaaatgaacAaccctgagacttgaggacacggctgtgtattactgtgcgagaaaaggccccagaagagatggctccctacagcaatgcgatatggacgtctggggccaagggaccacgatcaccgtctcctcagcatcccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 93 95 [301, 302] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>c,S35>T(+ - +)|a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)|a110>t,Y37>F(- + -)|g113>c,S38>T(+ - +)|a115>g,M39>V(+ - -)|a119>t,N40>I(- - -)|g139>a,G47>R(- - -)|g153>a|g157>a,V53>I(+ - +)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>c,S57>T(+ - +)|g173>c,S58>T(+ - +)|g176>a,S59>N(- - -)|a187>g,S63>G(+ + -)|t190>c,Y64>L(- - -)|a191>t,Y64>L(- - -)|t194>c,I65>T(- - -)|a195>g,I65>T(- - -)|g213>a|a214>g,K72>E(+ - -)|c230>a,T77>N(- + -)|a240>g|g252>c,K84>N(+ - -)|c255>t|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|g278>c,S93>T(+ - +)|g286>c,A96>L(+ - +)|c287>t,A96>L(+ - +)|c288>t,A96>L(+ - +)| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>c,S35>T(+ - +)|a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)|a110>t,Y37>F(- + -)|g113>c,S38>T(+ - +)| a115>g,M39>V(+ - -)|a119>t,N40>I(- - -)|g139>a,G47>R(- - -)|g153>a|g157>a,V53>I(+ - +)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>c,S57>T(+ - +)|g173>c,S58>T(+ - +)|g176>a,S59>N(- - -)|a187>g,S63>G(+ + -)|t190>c,Y64>L(- - -)|a191>t,Y64>L(- - -)|t194>c,I65>T(- - -)|a195>g,I65>T(- - -)| g213>a|a214>g,K72>E(+ - -)|c230>a,T77>N(- + -)|a240>g|g252>c,K84>N(+ - -)|c255>t|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|g278>c,S93>T(+ - +)|g286>c,A96>L(+ - +)|c287>t,A96>L(+ - +)|c288>t,A96>L(+ - +)| g319>a| 318 (320) 220 (222) 185 (186) 35 (36) 4 (5) 31 7 5 (6) 6 3 0 1 4 0 7 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 0 7 1 0 2 0 0 0 1 0 2 1 0 0 51 51 46 5 1 4 1 3 0 0 0 0 1 0 0 0 0 0 30 21 11 10 0 10 3 1 0 3 0 0 1 0 2 0 0 0 117 114 101 13 3 10 2 1 4 0 0 1 1 0 3 1 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagtttcactggttttacc gtgatctgggtccgccaggctccaaggaaggggctggaatggatctcatcc gtcactactaatggtctcacg tactacgcagactcagtagagggccgattcaacatctccagggacaacgccaacaatttagtgtttctgcaaatgaacaccctgagacttgaggacacggctgtgtattactgt gcgagaaaaggccccagaagagatggctccctacagcaatgcgatatggacgtc ARKGPRRDGSLQQCDMDV IGHV3-21 IGHD5-24 IGHJ6 ggattcagtttcactggttttacc gtgatctgggtccgccaggctccaaggaaggggctggaatggatctcatcc gtcactactaatggtctcacg tactacgcagactcagtagagggccgattcaacatctccagggacaacgccaacaatttagtgtttctgcaaatgaacaccctgagacttgaggacacggctgtgtattactgt gcgagaaaaggccccagaagagatggctccctacagcaatgcgatatggacgtc IGA2 35 210 21 14 11 21 10 14 4 0 14 17 JY8QFUQ01BLAI9 9538 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 979 95.77 204/213 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD3-10*01 F 2 8 8 17 8.8.17 [X.17.38.11] CAKKYHYGSGSYLYCFDYW in-frame - ggattcacctttagcaactttgccatgacctgggtccgccaggctccagggaagggactggagtgggtctcaactattagtggtggtgatgatagcacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatatcactgtgcgaagaagtatcactatggttcggggagttatctctactgctttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 100 [295] g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)|g119>c,S40>T(+ - +)|g147>a|g163>a,A55>T(- - -)|a175>g,S59>G(+ + -)|g185>a,G62>D(- - -)|g188>a,G63>D(- - -)|t307>c,Y103>H(+ - -)| g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)| g119>c,S40>T(+ - +)|g147>a|g163>a,A55>T(- - -)| a175>g,S59>G(+ + -)|g185>a,G62>D(- - -)|g188>a,G63>D(- - -)| t307>c,Y103>H(+ - -)| a318>g|g319>a| 317 (320) 218 (221) 209 (210) 9 (11) 1 (3) 8 1 (2) 5 (6) 0 1 0 0 1 0 1 0 0 0 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 1 0 0 0 0 0 51 51 48 3 1 2 0 2 0 0 0 0 0 0 1 0 0 0 30 24 21 3 0 3 1 2 0 0 0 0 0 0 0 0 0 0 117 114 113 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ggattcacctttagcaactttgcc atgacctgggtccgccaggctccagggaagggactggagtgggtctcaact attagtggtggtgatgatagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatatcactgt gcgaagaagtatcactatggttcggggagttatctctactgctttgactac AKKYHYGSGSYLYCFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcacctttagcaactttgcc atgacctgggtccgccaggctccagggaagggactggagtgggtctcaact attagtggtggtgatgatagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatatcactgt gcgaagaagtatcactatggttcggggagttatctctactgctttgactac IGA1 9 213 7 2 5 6 2 3 1 0 3 5 JY8QFUQ01BLEDM 8150 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 910 90.87 199/219 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-12*01 F 3 8 8 15 8.8.15 [2.17.38.11] CVRGGVSGAFRWGFDYW in-frame - gcctctggattcaccttcagtggttactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgaattgatagttctgcgaatctcatcaaatacgcggactccgtggagggccgattcaccgtctccagagacaacgccaagaacacggtgtatctgcaaatgaacagtctgagagccgacgacacggctgtttactactgtgtgagagggggagtcagtggggccttcaggtgggggtttgactactggggccagggaaccctggccaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgcttcaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 86 88 [296] a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)|t165>a|a169>g,N57>D(+ + -)|g175>t,D59>S(- - -)|a176>c,D59>S(- - -)|g185>c,G62>A(- + -)|g188>a,S63>N(- - -)|a190>c,S64>L(- - -)|g191>t,S64>L(- - -)|c194>t,T65>I(- - -)|a195>c,T65>I(- - -)|g197>a,S66>K(- - -)|c198>a,S66>K(- - -)|a214>g,K72>E(+ - -)|a232>g,I78>V(+ - +)|c259>g,L87>V(+ - +)|g291>c,E97>D(+ - +)|g303>t|t306>c|c314>t,A105>V(+ - +)|a315>g,A105>V(+ - +)| a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)| t165>a| a169>g,N57>D(+ + -)|g175>t,D59>S(- - -)|a176>c,D59>S(- - -)|g185>c,G62>A(- + -)|g188>a,S63>N(- - -)|a190>c,S64>L(- - -)|g191>t,S64>L(- - -)|c194>t,T65>I(- - -)|a195>c,T65>I(- - -)| g197>a,S66>K(- - -)|c198>a,S66>K(- - -)|a214>g,K72>E(+ - -)|a232>g,I78>V(+ - +)|c259>g,L87>V(+ - +)|g291>c,E97>D(+ - +)|g303>t|t306>c| c314>t,A105>V(+ - +)|a315>g,A105>V(+ - +)|a320>g| 319 (320) 226 (227) 204 22 (23) 3 (4) 19 5 (6) 2 3 1 3 1 0 1 2 1 3 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 24 15 9 0 9 1 1 1 0 3 0 0 0 1 0 2 0 117 114 106 8 2 6 2 1 0 1 0 1 0 0 1 1 1 0 7 (8) 7 5 2 (3) 0 (1) 2 1 (2) 0 1 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcaccttcagtggttactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacga attgatagttctgcgaatctcatc aaatacgcggactccgtggagggccgattcaccgtctccagagacaacgccaagaacacggtgtatctgcaaatgaacagtctgagagccgacgacacggctgtttactactgt gtgagagggggagtcagtggggccttcaggtgggggtttgactac VRGGVSGAFRWGFDY IGHV3-74 IGHD5-12 IGHJ4 ggattcaccttcagtggttactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacga attgatagttctgcgaatctcatc aaatacgcggactccgtggagggccgattcaccgtctccagagacaacgccaagaacacggtgtatctgcaaatgaacagtctgagagccgacgacacggctgtttactactgt gtgagagggggagtcagtggggccttcaggtgggggtttgactac IGG2 20 213 9 11 4 11 5 9 3 0 6 11 JY8QFUQ01BLGCA 18707 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 962 94.42 203/215 nt NA Homsap IGHJ5*02 F 246 98.04 50/51 nt Homsap IGHD4-17*01 F 3 8 7 16 8.7.16 [1.17.38.11] CARGSVTTWRRENWFDPW in-frame + tctctggtggctccatcagtacttattactggaactggatccggcagcccccagggaagggactggagtggattgggtatatccataacagtgggagtagcaactacaacccctccctcaagagtcgagtcaacatatctgtagacgcgtccaagaaccagttctccctgaagctgacctctgtgaccgctgcggacacggccgtttattactgtgcgagaggttcggtgactacgtggagacgggagaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [296, 295] g107>c,S36>T(+ - +)|c111>t|g119>a,S40>N(- - -)|t169>c,Y57>H(+ - -)|t172>a,Y58>N(- - -)|c192>t|c194>g,T65>S(+ - +)|c230>a,T77>N(- + -)|a237>t|a244>g,T82>A(- - -)|g275>c,S92>T(+ - +)|g303>t| g107>c,S36>T(+ - +)|c111>t| g119>a,S40>N(- - -)| t169>c,Y57>H(+ - -)|t172>a,Y58>N(- - -)|c192>t|c194>g,T65>S(+ - +)| c230>a,T77>N(- + -)|a237>t|a244>g,T82>A(- - -)|g275>c,S92>T(+ - +)|g303>t| a320>g| 319 (320) 222 (223) 210 12 (13) 4 (5) 8 1 (2) 1 2 1 0 1 1 1 2 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 1 0 0 0 0 0 1 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 21 17 4 1 3 0 0 1 1 0 0 0 1 0 1 0 0 117 114 109 5 2 3 1 0 0 0 0 1 1 0 1 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagtacttattac tggaactggatccggcagcccccagggaagggactggagtggattgggtat atccataacagtgggagtagc aactacaacccctccctcaagagtcgagtcaacatatctgtagacgcgtccaagaaccagttctccctgaagctgacctctgtgaccgctgcggacacggccgtttattactgt gcgagaggttcggtgactacgtggagacgggagaactggttcgacccc ARGSVTTWRRENWFDP IGHV4-59 IGHD4-17 IGHJ5 ggtggctccatcagtacttattac tggaactggatccggcagcccccagggaagggactggagtggattgggtat atccataacagtgggagtagc aactacaacccctccctcaagagtcgagtcaacatatctgtagacgcgtccaagaaccagttctccctgaagctgacctctgtgaccgctgcggacacggccgtttattactgt gcgagaggttcggtgactacgtggagacgggagaactggttcgacccc IGG1 12 210 5 7 3 8 2 4 2 2 4 4 JY8QFUQ01BLGYX 4007 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 894 91.12 195/214 nt 90.19 193/214 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD1-1*01 F 2 8 8 9 8.8.9 [0.17.38.11] CARRQLALAYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 55 to 55 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 70 of V-REGION: 'C' (1 nucleotides at position 115 to 115 in the user submitted sequence), (cause frameshift); tggatacacctttatcacctactggatcgcctgggtgcgccaaatgcccgggaaAaggcctggagttgatgggagtcatctatcctggtgactctgagaccagatacagcccgtCccttccaaggccacatcaccctctcagtcgacaagtccatcgataccgcctacctggagtggagcagcctgaaggcctcggacaccgccatgtacttctgtgcaagaagacaactggcccttgcctactggggccagggaacccaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [274] g86>c,S29>T(+ - +)|c104>t,T35>I(- - -)|g107>c,S36>T(+ - +)|g119>c,G40>A(- + -)|g132>a|g155>t,W52>L(+ - -)|g162>a|a163>g,I55>V(+ - +)|t192>g,D64>E(+ - +)|g225>c,Q75>H(- + -)|g226>a,V76>I(+ - +)|a232>c,I78>L(+ + +)|c239>t,A80>V(+ - +)|a253>g,S85>D(- - -)|g254>a,S85>D(- - -)|c255>t,S85>D(- - -)|c268>g,Q90>E(+ + -)|t306>c|a308>t,Y103>F(- + -)|g315>a| g86>c,S29>T(+ - +)|c104>t,T35>I(- - -)|g107>c,S36>T(+ - +)| g119>c,G40>A(- + -)|g132>a|g155>t,W52>L(+ - -)|g162>a|a163>g,I55>V(+ - +)| t192>g,D64>E(+ - +)| g225>c,Q75>H(- + -)|g226>a,V76>I(+ - +)|a232>c,I78>L(+ + +)|c239>t,A80>V(+ - +)|a253>g,S85>D(- - -)|g254>a,S85>D(- - -)|c255>t,S85>D(- - -)|c268>g,Q90>E(+ + -)|t306>c|a308>t,Y103>F(- + -)| g315>a|c319>a|a320>g| 318 (320) 220 (222) 200 20 (22) 4 (6) 16 2 (3) 5 3 1 1 0 (1) 1 0 4 1 1 1 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 1 0 0 0 0 0 2 0 0 0 51 51 46 5 2 3 1 2 0 0 0 0 0 0 1 0 1 0 30 24 23 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 117 114 104 10 1 9 1 2 2 1 1 0 1 0 1 1 0 0 6 (8) 6 5 1 (3) 1 (3) 0 0 (1) 1 0 0 0 0 (1) 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacacctttatcacctactgg atcgcctgggtgcgccaaatgcccgggaaaggcctggagttgatgggagtc atctatcctggtgactctgagacc agatacagcccgtccttccaaggccacatcaccctctcagtcgacaagtccatcgataccgcctacctggagtggagcagcctgaaggcctcggacaccgccatgtacttctgt gcaagaagacaactggcccttgcctac ARRQLALAY IGHV5-51 IGHD1-1 IGHJ4 ggatacacctttatcacctactgg atcgcctgggtgcgccaaatgcccgggaaaggcctggagttgatgggagtc atctatcctggtgactctgagacc agatacagcccgtccttccaaggccacatcaccctctcagtcgacaagtccatcgataccgcctacctggagtggagcagcctgaaggcctcggacaccgccatgtacttctgt gcaagaagacaactggcccttgcctac IGA1 19 213 10 9 7 13 3 6 3 0 12 4 JY8QFUQ01BLTJO 3302 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 815 86.24 188/218 nt NA Homsap IGHJ6*02 F (see comment) 184 77.42 48/62 nt Homsap IGHD2-8*01 F 3 8 8 20 8.8.20 [1.17.38.11] CAKGASTAVMVSGTDCYGLDVW in-frame - Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) or Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) cctctggattccccttcagcacctatcccatgagctgggtccgccaggctccagggaggggactggagtgggtcgcaactataagtggtggaggttatagtatatatgacgcagactccgtgaagggcaggttcaccatctccagagacaactccaagaccaccttgtttctggaaatgaaaagtctgagagtcgatgatacggccgtctattactgtgcgaaaggggcctcgactgcagtaatggtgtctggaacagattgttacggtttggacgtctggggccacgggaccgcggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [309] a85>c,T29>P(+ + -)|t90>c|g107>c,S36>T(+ - +)|g112>c,A38>P(- - -)|a143>g,K48>R(+ + +)|g147>a|t160>g,S54>A(- + -)|g163>a,A55>T(- - -)|t168>a|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|c192>t|c194>t,T65>I(- - -)|c198>t|t199>g,Y67>D(- - -)|c223>a|t246>c|a254>c,N85>T(- + -)|g258>c|c259>t|a263>t,Y88>F(- + -)|c268>g,Q90>E(+ + -)|c276>a,N92>K(+ - -)|c279>t|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)|c294>t|a303>c| a85>c,T29>P(+ + -)|t90>c|g107>c,S36>T(+ - +)|g112>c,A38>P(- - -)| a143>g,K48>R(+ + +)|g147>a|t160>g,S54>A(- + -)|g163>a,A55>T(- - -)| t168>a|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|c192>t|c194>t,T65>I(- - -)| c198>t|t199>g,Y67>D(- - -)|c223>a|t246>c|a254>c,N85>T(- + -)|g258>c|c259>t|a263>t,Y88>F(- + -)|c268>g,Q90>E(+ + -)|c276>a,N92>K(+ - -)|c279>t|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)|c294>t|a303>c| a320>g| 319 (320) 225 (226) 195 30 (31) 12 (13) 18 2 (3) 3 7 2 3 2 1 2 3 1 2 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 0 1 1 0 0 0 2 0 0 0 51 51 47 4 1 3 1 2 0 0 0 0 0 0 0 0 0 1 30 24 17 7 2 5 1 1 2 0 0 0 0 2 0 0 1 0 117 114 99 15 8 7 0 0 5 1 2 2 1 0 1 1 1 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattccccttcagcacctatccc atgagctgggtccgccaggctccagggaggggactggagtgggtcgcaact ataagtggtggaggttatagtata tatgacgcagactccgtgaagggcaggttcaccatctccagagacaactccaagaccaccttgtttctggaaatgaaaagtctgagagtcgatgatacggccgtctattactgt gcgaaaggggcctcgactgcagtaatggtgtctggaacagattgttacggtttggacgtc AKGASTAVMVSGTDCYGLDV IGHV3-23 IGHD2-8 IGHJ6 ggattccccttcagcacctatccc atgagctgggtccgccaggctccagggaggggactggagtgggtcgcaact ataagtggtggaggttatagtata tatgacgcagactccgtgaagggcaggttcaccatctccagagacaactccaagaccaccttgtttctggaaatgaaaagtctgagagtcgatgatacggccgtctattactgt gcgaaaggggcctcgactgcagtaatggtgtctggaacagattgttacggtttggacgtc IGA1 30 213 14 16 10 18 4 12 9 3 10 8 JY8QFUQ01BLX8G 21527 productive (see comment) Homsap IGHV1-46*01 F, or Homsap IGHV1-46*03 F 1011 97.2 208/214 nt 96.26 206/214 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-13*01 F 3 8 8 11 8.8.11 [0.17.38.11] CARGQQLRDFDYW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 38 of V-REGION: 'T' (1 nucleotides at position 25 to 25 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 46 to 46 in the user submitted sequence), (cause frameshift); tggatacaccttcaccagctactaTtttgcactgggtgcgacaggCcccctggacaagggcttgagtggatgggaataatcgaccctagtggtggtgccacaagctacgcacagcagttccagggcagagtcaccatgaccagggacacgtccacgagcacagtctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgtgcgaggggacagcagcttcgagactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [280] a115>t,M39>L(+ + -)|a169>g,N57>D(+ + -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|a208>c,K70>Q(+ - -)|g291>a| a115>t,M39>L(+ + -)| a169>g,N57>D(+ + -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| a208>c,K70>Q(+ - -)|g291>a| a318>g|a320>g| 317 (320) 219 (222) 213 (214) 6 (8) 1 (3) 5 2 (4) 1 0 0 1 0 1 0 1 0 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 30 24 21 3 0 3 2 0 0 0 0 0 0 0 1 0 0 0 117 114 112 2 1 1 0 1 0 0 1 0 0 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,162-163(FR2)|ta,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,62-63(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,164-165(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggca,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|aacc,169-172(CDR2)|agct,196-199(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcaccagctactat ttgcactgggtgcgacaggcccctggacaagggcttgagtggatgggaata atcgaccctagtggtggtgccaca agctacgcacagcagttccagggcagagtcaccatgaccagggacacgtccacgagcacagtctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcgaggggacagcagcttcgagactttgactac ARGQQLRDFDY IGHV1-46 IGHD6-13 IGHJ4 ggatacaccttcaccagctactat ttgcactgggtgcgacaggcccctggacaagggcttgagtggatgggaata atcgaccctagtggtggtgccaca agctacgcacagcagttccagggcagagtcaccatgaccagggacacgtccacgagcacagtctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcgaggggacagcagcttcgagactttgactac IGA2 6 213 3 3 1 2 2 4 1 0 2 3 JY8QFUQ01BM58O 11538 productive Homsap IGHV3-11*01 F 882 89.86 195/217 nt NA Homsap IGHJ2*01 F 220 90.57 48/53 nt Homsap IGHD2-8*02 F 3 8 8 12 8.8.12 [1.17.38.11] CVDIRLGDWFFDLW in-frame + ctctggattcatcttcagtgaccactacatgagctgggtccgccaggctccggggaagggtctggagtggatctcatacatcagtacaagtggtaatatggtttattacgcggactctgtgaagggccgattcaccgtctccagggacaacgccaagaactcactgtatctgcaaatgaacggcctcagagtcgaggacacggccgtctattactgtgtcgacataagactgggtgattggttcttcgatctctggggccgtggcaccctggtaactgtctcctcagcatcccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [283] c86>t,T29>I(- - -)|t109>c,Y37>H(+ - -)|a124>g,I42>V(+ - +)|a138>g|g147>t|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|t168>c|g173>c,S58>T(+ - +)|t174>a,S58>T(+ - +)|g188>a,S63>N(- - -)|c191>t,T64>M(- - -)|c192>g,T64>M(- - -)|a193>g,I65>V(+ - +)|a195>t,I65>V(+ - +)|c198>t|a204>g|a232>g,I78>V(+ - +)|a277>g,S93>G(+ + -)|g282>c|c287>t,A96>V(+ - +)|g303>c| c86>t,T29>I(- - -)|t109>c,Y37>H(+ - -)| a124>g,I42>V(+ - +)|a138>g|g147>t|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)| t168>c|g173>c,S58>T(+ - +)|t174>a,S58>T(+ - +)|g188>a,S63>N(- - -)|c191>t,T64>M(- - -)|c192>g,T64>M(- - -)|a193>g,I65>V(+ - +)|a195>t,I65>V(+ - +)| c198>t|a204>g|a232>g,I78>V(+ - +)|a277>g,S93>G(+ + -)|g282>c|c287>t,A96>V(+ - +)|g303>c| c314>t,A105>V(+ - +)|g315>c,A105>V(+ - +)|a316>g,R106>D(+ - -)|g317>a,R106>D(+ - -)|a318>c,R106>D(+ - -)|g319>a|a320>t| 313 (320) 218 (225) 196 22 (29) 7 (9) 15 (20) 6 (7) 2 (4) 4 (5) 3 0 (1) 0 1 (2) 1 3 (4) 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 1 1 0 0 0 0 0 0 0 0 51 51 46 5 2 3 2 1 0 1 0 0 0 0 0 0 1 0 30 24 16 8 1 7 1 1 1 1 0 0 1 1 1 1 0 0 117 114 107 7 4 3 3 0 2 0 0 0 0 0 2 0 0 0 1 (8) 1 1 0 (7) 0 (2) 0 (5) 0 (1) 0 (2) 0 (1) 0 0 (1) 0 0 (1) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagtgaccactac atgagctgggtccgccaggctccggggaagggtctggagtggatctcatac atcagtacaagtggtaatatggtt tattacgcggactctgtgaagggccgattcaccgtctccagggacaacgccaagaactcactgtatctgcaaatgaacggcctcagagtcgaggacacggccgtctattactgt gtcgacataagactgggtgattggttcttcgatctc VDIRLGDWFFDL IGHV3-11 IGHD2-8 IGHJ2 ggattcatcttcagtgaccactac atgagctgggtccgccaggctccggggaagggtctggagtggatctcatac atcagtacaagtggtaatatggtt tattacgcggactctgtgaagggccgattcaccgtctccagggacaacgccaagaactcactgtatctgcaaatgaacggcctcagagtcgaggacacggccgtctattactgt gtcgacataagactgggtgattggttcttcgatctc IGA1 22 213 15 7 6 11 9 11 6 1 6 9 JY8QFUQ01BM6Z0 3053 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 850 88.43 191/216 nt NA Homsap IGHJ5*02 F 228 94.12 48/51 nt Homsap IGHD6-6*01 F 2 8 8 14 8.8.14 [1.17.38.11] CAKCKAPGKVDWFDPW in-frame - tctggattcacctttagcggctatgccatggcttgggtccgccaggctccagggaaggggctggagtgggtctcaactagtactactgatggagctggcccatactacgcagactccgtgaggggccggttcaccgtcttcagagacaattccaagaacactctgtatctacaaatggacaccctgagagccgacgacacggccatgtattactgtgcgaaatgtaaagcgcctggtaaggtggactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [289, 288] a106>g,S36>G(+ + -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c120>t,S40>A(- + -)|g163>a,A55>T(- - -)|t167>g,I56>S(- - -)|g170>c,S57>T(+ - +)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|t186>a|g188>c,G63>A(- + -)|a190>g,S64>G(+ + -)|a193>c,T65>P(+ + -)|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|c236>t,S79>F(- - -)|g258>t|g267>a|a274>g,N92>D(+ + -)|g278>c,S93>T(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>M(+ - -)|a303>g,V101>M(+ - -)| a106>g,S36>G(+ + -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c120>t,S40>A(- + -)|g163>a,A55>T(- - -)| t167>g,I56>S(- - -)|g170>c,S57>T(+ - +)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|t186>a|g188>c,G63>A(- + -)|a190>g,S64>G(+ + -)|a193>c,T65>P(+ + -)| a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|c236>t,S79>F(- - -)|g258>t|g267>a|a274>g,N92>D(+ + -)|g278>c,S93>T(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>M(+ - -)|a303>g,V101>M(+ - -)| g319>t|a320>g| 318 (320) 222 (224) 197 25 (27) 3 (5) 22 8 (9) 5 2 0 1 0 0 1 6 0 1 (2) 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 51 47 4 0 4 1 1 1 0 0 0 0 0 1 0 0 0 30 24 14 10 1 9 2 2 0 0 1 0 0 1 3 0 0 1 117 114 104 10 2 8 4 2 1 0 0 0 0 0 2 0 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcacctttagcggctatgcc atggcttgggtccgccaggctccagggaaggggctggagtgggtctcaact agtactactgatggagctggccca tactacgcagactccgtgaggggccggttcaccgtcttcagagacaattccaagaacactctgtatctacaaatggacaccctgagagccgacgacacggccatgtattactgt gcgaaatgtaaagcgcctggtaaggtggactggttcgacccc AKCKAPGKVDWFDP IGHV3-23 IGHD6-6 IGHJ5 ggattcacctttagcggctatgcc atggcttgggtccgccaggctccagggaaggggctggagtgggtctcaact agtactactgatggagctggccca tactacgcagactccgtgaggggccggttcaccgtcttcagagacaattccaagaacactctgtatctacaaatggacaccctgagagccgacgacacggccatgtattactgt gcgaaatgtaaagcgcctggtaaggtggactggttcgacccc IGA2 25 213 15 10 7 14 8 11 2 1 12 10 JY8QFUQ01BM80W 13432 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 912 91.59 196/214 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD3-10*01 F 2 8 8 17 8.8.17 [0.17.38.11] CAKKYHYGSGTYLYYFDYW in-frame + tggattcacctttagccactttgccgtgacctgggtccgccaggctccagggaagggtctggaatgggtctcaactattagcggtagtgatggtagcaagtactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacaccctatatctgcaaatgaccagcctgagagccgaggacacggccgtatatttctgcgcgaagaagtatcactatggttcggggacttacttgtactattttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [296] a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a110>t,Y37>F(- + -)|a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|g147>t|g153>a|g163>a,A55>T(- - -)|t171>c|g185>a,G62>D(- - -)|c194>a,T65>K(- - -)|a195>g,T65>K(- - -)|a215>g,K72>R(+ + +)|g258>c|g261>a|a275>c,N92>T(- + -)|a308>t,Y103>F(- + -)|t312>c| a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a110>t,Y37>F(- + -)| a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|g147>t|g153>a|g163>a,A55>T(- - -)| t171>c|g185>a,G62>D(- - -)|c194>a,T65>K(- - -)|a195>g,T65>K(- - -)| a215>g,K72>R(+ + +)|g258>c|g261>a|a275>c,N92>T(- + -)|a308>t,Y103>F(- + -)|t312>c| a318>g|g319>a| 317 (320) 219 (222) 201 (202) 18 (20) 6 (8) 12 3 (4) 5 (6) 0 2 2 1 2 0 2 0 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 1 0 1 0 0 0 0 0 51 51 46 5 2 3 1 2 0 0 0 0 0 0 1 0 1 0 30 24 20 4 1 3 1 1 0 1 0 1 0 0 0 0 0 0 117 114 108 6 3 3 1 1 0 1 1 0 1 0 1 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t ggattcacctttagccactttgcc gtgacctgggtccgccaggctccagggaagggtctggaatgggtctcaact attagcggtagtgatggtagcaag tactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacaccctatatctgcaaatgaccagcctgagagccgaggacacggccgtatatttctgc gcgaagaagtatcactatggttcggggacttacttgtactattttgactac AKKYHYGSGTYLYYFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcacctttagccactttgcc gtgacctgggtccgccaggctccagggaagggtctggaatgggtctcaact attagcggtagtgatggtagcaag tactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacaccctatatctgcaaatgaccagcctgagagccgaggacacggccgtatatttctgc gcgaagaagtatcactatggttcggggacttacttgtactattttgactac IGA1 18 213 10 8 5 9 5 9 5 1 6 6 JY8QFUQ01BM96X 10205 productive Homsap IGHV3-9*01 F 927 92.17 200/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD6-13*01 F 1 8 8 14 8.8.14 [1.17.38.11] CAKDWPSSGWSHFEAW in-frame + ctctggattcacctttgatgagcatgccatgcactgggtccggcaagttccggggaagggcctggagtgggtctctggtgttacttggaatagtggtgtcatagactatgcggactctgtgaagggccgattcaccatctccagagacaacgccaggaattccctgtatctacaaatgaacagtctgagaactgatgacacggccttctatttctgtgcaaaagattggccgagcagcggctggtcccattttgaggcttggggccagggagtcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcaccccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 79 95 [290, 291] t108>g,D36>E(+ - +)|t109>c,Y37>H(+ - -)|c134>t,A45>V(+ - +)|a138>g|a162>t|a166>g,I56>V(+ - +)|g170>c,S57>T(+ - +)|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|g197>a,G66>D(- - -)|a251>g,K84>R(+ + +)|c255>t|g267>a|g286>a,A96>T(- - -)|g291>t,E97>D(+ - +)|g303>c,L101>F(+ - -)|a308>t,Y103>F(- + -)| t108>g,D36>E(+ - +)|t109>c,Y37>H(+ - -)| c134>t,A45>V(+ - +)|a138>g|a162>t| a166>g,I56>V(+ - +)|g170>c,S57>T(+ - +)|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)| g197>a,G66>D(- - -)|a251>g,K84>R(+ + +)|c255>t|g267>a|g286>a,A96>T(- - -)|g291>t,E97>D(+ - +)|g303>c,L101>F(+ - -)|a308>t,Y103>F(- + -)| a322>t| 321 (322) 226 (227) 209 17 (18) 4 (5) 13 4 3 2 1 0 0 2 (3) 0 2 0 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 1 0 0 0 0 0 0 0 1 51 51 48 3 2 1 1 0 1 0 0 0 1 0 0 0 0 0 30 24 20 4 0 4 2 0 0 0 0 0 0 0 1 0 1 0 117 114 106 8 2 6 1 3 1 0 0 0 1 0 1 0 1 0 9 (10) 9 9 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttgatgagcatgcc atgcactgggtccggcaagttccggggaagggcctggagtgggtctctggt gttacttggaatagtggtgtcata gactatgcggactctgtgaagggccgattcaccatctccagagacaacgccaggaattccctgtatctacaaatgaacagtctgagaactgatgacacggccttctatttctgt gcaaaagattggccgagcagcggctggtcccattttgaggct AKDWPSSGWSHFEA IGHV3-9 IGHD6-13 IGHJ4 ggattcacctttgatgagcatgcc atgcactgggtccggcaagttccggggaagggcctggagtgggtctctggt gttacttggaatagtggtgtcata gactatgcggactctgtgaagggccgattcaccatctccagagacaacgccaggaattccctgtatctacaaatgaacagtctgagaactgatgacacggccttctatttctgt gcaaaagattggccgagcagcggctggtcccattttgaggct IGA1 17 213 10 7 5 9 5 8 4 0 7 6 JY8QFUQ01BMLGO 15447 productive Homsap IGHV3-23*05 F 815 86.24 188/218 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-19*01 F 1 8 8 14 8.8.14 [1.17.38.11] CVKSTGHSGWYPFDNW in-frame + cctctggattcacattcagcaattatgccatgggctgggtccgccaggctccagggaaggggctggagtgggtcgctgctattgatggcagtggtgaaagaactcactatgcagactccgaacagggccgcgtcaacatctctagagacaattccaagaacatgatatatgtgcaattgagcagcctgagagccgaggacacggccatgtattactgtgtgaagtccaccgggcacagtggctggtatccctttgacaactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 93 100 [291, 292] c87>a|t90>c|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a118>g,S40>G(+ + -)|t160>g,S54>A(- + -)|a162>t,S54>A(- + -)|t169>g,Y57>D(- - -)|a172>g,S58>G(+ + -)|a187>g,S63>E(- - -)|g188>a,S63>E(- - -)|t189>a,S63>E(- - -)|c192>a,S64>R(- - -)|a195>t|t196>c,Y66>H(+ - -)|t212>a,V71>E(- + -)|g213>a,V71>E(- + -)|a214>c,K72>Q(+ - -)|g225>c|t226>g,F76>V(+ - -)|c230>a,T77>N(- + -)|c237>t|c257>t,T86>M(- - -)|c259>a,L87>I(+ + +)|g261>a,L87>I(+ + +)|c265>g,L89>V(+ - +)|a271>t,M91>L(+ + -)|a275>g,N92>S(- - -)|g301>a,V101>M(+ - -)|a303>g,V101>M(+ - -)|c314>t,A105>V(+ - +)| c87>a|t90>c|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a118>g,S40>G(+ + -)|t160>g,S54>A(- + -)|a162>t,S54>A(- + -)| t169>g,Y57>D(- - -)|a172>g,S58>G(+ + -)|a187>g,S63>E(- - -)|g188>a,S63>E(- - -)|t189>a,S63>E(- - -)|c192>a,S64>R(- - -)|a195>t| t196>c,Y66>H(+ - -)|t212>a,V71>E(- + -)|g213>a,V71>E(- + -)|a214>c,K72>Q(+ - -)|g225>c|t226>g,F76>V(+ - -)|c230>a,T77>N(- + -)|c237>t|c257>t,T86>M(- - -)|c259>a,L87>I(+ + +)|g261>a,L87>I(+ + +)|c265>g,L89>V(+ - +)|a271>t,M91>L(+ + -)|a275>g,N92>S(- - -)|g301>a,V101>M(+ - -)|a303>g,V101>M(+ - -)| c314>t,A105>V(+ - +)|a318>g| 317 (318) 223 (224) 192 31 (32) 5 (6) 26 5 (6) 5 4 2 1 4 3 2 1 1 0 3 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 1 1 1 0 1 0 0 0 0 0 0 51 51 48 3 0 3 1 0 0 0 0 0 1 0 0 0 0 1 30 24 17 7 1 6 2 1 0 0 0 1 1 1 0 0 0 1 117 114 98 16 2 14 2 3 2 1 1 2 1 1 1 1 0 1 5 (6) 5 4 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|agca,172-175(CDR2)|ggta,184-187(CDR2)|agca,190-193(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacattcagcaattatgcc atgggctgggtccgccaggctccagggaaggggctggagtgggtcgctgct attgatggcagtggtgaaagaact cactatgcagactccgaacagggccgcgtcaacatctctagagacaattccaagaacatgatatatgtgcaattgagcagcctgagagccgaggacacggccatgtattactgt gtgaagtccaccgggcacagtggctggtatccctttgacaac VKSTGHSGWYPFDN IGHV3-23 IGHD6-19 IGHJ4 ggattcacattcagcaattatgcc atgggctgggtccgccaggctccagggaaggggctggagtgggtcgctgct attgatggcagtggtgaaagaact cactatgcagactccgaacagggccgcgtcaacatctctagagacaattccaagaacatgatatatgtgcaattgagcagcctgagagccgaggacacggccatgtattactgt gtgaagtccaccgggcacagtggctggtatccctttgacaac IGA2 30 213 15 15 8 14 7 16 2 3 17 8 JY8QFUQ01BMOB5 10416 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 990 95.39 207/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 8 8 8.8.8 [1.17.38.11] CARDNYGDYW in-frame + ctctggattcaccttcagtaactatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattagtagtagtgctaggtacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagcccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcc NA IGA2 100 95 [271, 272] g107>a,S36>N(- - -)|a184>g,S62>A(- + -)|g185>c,S62>A(- + -)|t189>g,S63>R(- - -)|t256>a,S86>T(+ - +)|a258>t,S86>T(+ - +)|g261>c|g267>c|c279>t|g303>a|g315>a| g107>a,S36>N(- - -)| a184>g,S62>A(- + -)|g185>c,S62>A(- + -)|t189>g,S63>R(- - -)| t256>a,S86>T(+ - +)|a258>t,S86>T(+ - +)|g261>c|g267>c|c279>t|g303>a| g315>a| 320 225 214 11 5 6 1 3 1 0 0 0 1 1 3 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 1 0 0 1 117 114 108 6 4 2 0 1 1 0 0 0 1 1 2 0 0 0 8 8 7 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtaactatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtgctaggtacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-21 IGHD2-8 IGHJ4 ggattcaccttcagtaactatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtagtagtgctaggtacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac IGA2 10 213 4 6 3 6 1 4 4 0 2 4 JY8QFUQ01BMQ8K 17380 productive Homsap IGHV3-7*01 F 939 93.46 200/214 nt NA Homsap IGHJ4*02 F (see comment) 213 93.75 45/48 nt Homsap IGHD2-15*01 F 2 8 8 14 8.8.14 [0.17.38.11] CARVRGYYGGQYFDSW in-frame - Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) tggattcacctttagtagttattggatgacctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaaggaagatgggaatgataaatactatgtcgactctgtgaggggccggttcaccatctccagagacaacgccaagagctcactgtttctgcaagtgaacagcctgagagccgacgacacggctgtttattactgtgcgagagttcgaggttattacggtgggcagtactttgattcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [287, 286] c108>t|g119>c,S40>T(+ - +)|c172>g,Q58>E(+ + -)|a186>g|g188>a,S63>N(- - -)|g192>t,E64>D(+ - +)|g204>c|a215>g,K72>R(+ + +)|a225>g|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|a271>g,M91>V(+ - -)|g291>c,E97>D(+ - +)|g303>t| c108>t| g119>c,S40>T(+ - +)| c172>g,Q58>E(+ + -)|a186>g|g188>a,S63>N(- - -)|g192>t,E64>D(+ - +)| g204>c|a215>g,K72>R(+ + +)|a225>g|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|a271>g,M91>V(+ - -)|g291>c,E97>D(+ - +)|g303>t| a320>t| 319 (320) 221 (222) 207 14 (15) 5 (6) 9 5 1 1 0 0 0 1 (2) 0 3 1 2 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 20 4 1 3 1 1 0 0 0 0 0 0 0 1 1 0 117 114 106 8 3 5 4 0 0 0 0 0 1 0 2 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| t ggattcacctttagtagttattgg atgacctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatgggaatgataaa tactatgtcgactctgtgaggggccggttcaccatctccagagacaacgccaagagctcactgtttctgcaagtgaacagcctgagagccgacgacacggctgtttattactgt gcgagagttcgaggttattacggtgggcagtactttgattcc ARVRGYYGGQYFDS IGHV3-7 IGHD2-15 IGHJ4 ggattcacctttagtagttattgg atgacctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatgggaatgataaa tactatgtcgactctgtgaggggccggttcaccatctccagagacaacgccaagagctcactgtttctgcaagtgaacagcctgagagccgacgacacggctgtttattactgt gcgagagttcgaggttattacggtgggcagtactttgattcc IGA2 14 213 7 7 2 8 5 6 3 2 6 3 JY8QFUQ01BN0IG 14858 productive Homsap IGHV1-24*01 F 988 96.24 205/213 nt NA Homsap IGHJ5*02 F 141 77.08 37/48 nt Homsap IGHD5-18*01 F 1 8 8 7 8.8.7 [X.17.38.11] CAIGAMATW in-frame - ggaaaaaccctcactgaagtatccatgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggaggatttgatcctgaagatggtgaaataatctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgtgcaataggagctatggccacctggggccagggaaccctggtcaccgtcgactcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [265] t82>a,Y28>K(- - -)|c84>a,Y28>K(- - -)|t109>g,L37>V(+ - +)|t165>a|c194>t,T65>I(- - -)|g226>a,V76>I(+ - +)|a232>g,M78>V(+ - -)|g291>a|c317>t,T106>I(- - -)| t82>a,Y28>K(- - -)|c84>a,Y28>K(- - -)|t109>g,L37>V(+ - +)| t165>a| c194>t,T65>I(- - -)| g226>a,V76>I(+ - +)|a232>g,M78>V(+ - -)|g291>a| c317>t,T106>I(- - -)|a320>g| 319 (320) 220 (221) 211 9 (10) 2 (3) 7 1 (2) 2 2 0 0 1 0 2 0 0 0 1 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 0 0 1 0 1 0 0 0 1 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 117 114 111 3 1 2 1 2 0 0 0 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,14-15(FR1)|aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|aa,107-108(CDR1)|ta,110-111(CDR1)|aa,141-142(FR2)|aa,143-144(FR2)|aa,176-177(CDR2)|aa,191-192(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,249-250(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,14-15(FR1)|tt,74-75(FR1)|ta,82-83(CDR1)|tt,109-110(CDR1)|tt,149-150(FR2)|tt,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,249-250(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggta,12-15(FR1)|ggct,24-27(FR1)|ggtt,72-75(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,163-166(FR2-CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ggaaaaaccctcactgaagtatcc atgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggagga tttgatcctgaagatggtgaaata atctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcaataggagctatggccacc AIGAMAT IGHV1-24 IGHD5-18 IGHJ5 ggaaaaaccctcactgaagtatcc atgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggagga tttgatcctgaagatggtgaaata atctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcaataggagctatggccacc IGA2 8 213 4 4 3 4 1 4 2 0 2 4 JY8QFUQ01BN1A6 8384 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 847 87.67 192/219 nt NA Homsap IGHJ6*02 F (see comment) 193 79.03 49/62 nt Homsap IGHD6-6*01 F 1 8 8 17 8.8.17 [2.17.38.11] CVRDSGPSGYARYESMDVW in-frame - Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) gcctctggattcagcctcacttcctatggcatgaactgggtccgccaggctccagggagggggctggagtgggtctcacacgttaatatgggtagtactcacatatactacgtaggctccgtgaggggccgattcaccatctccagagacgacgccaagaactcagtgtatctgcagatgaacaacttgagagccgaggacacggctctatattactgtgtgagagatagtggcccgtccggctacgctaggtacgagtctatggacgtctggggccaagggaccgcggtcaccgtctcctcagcctccaccaagggcatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 88 [300] c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|g104>c,S35>T(+ - +)|a106>t|g107>c|a112>g,S38>G(+ + -)|a143>g,K48>R(+ + +)|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)|a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|g173>t,S58>M(- - -)|t174>g,S58>M(- - -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)|c203>t,A68>V(+ - +)|a206>g,D69>G(- - -)|a210>c|a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|c259>g,L87>V(+ - +)|a270>g|g278>a,S93>N(- - -)|c280>t|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)|c314>t,A105>V(+ - +)| c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|g104>c,S35>T(+ - +)|a106>t|g107>c|a112>g,S38>G(+ + -)| a143>g,K48>R(+ + +)|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)| a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|g173>t,S58>M(- - -)|t174>g,S58>M(- - -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)| c203>t,A68>V(+ - +)|a206>g,D69>G(- - -)|a210>c|a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|c259>g,L87>V(+ - +)|a270>g|g278>a,S93>N(- - -)|c280>t|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| c314>t,A105>V(+ - +)| 320 227 199 28 5 23 8 3 3 3 1 1 1 0 4 2 1 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 2 4 1 0 0 1 0 0 1 0 2 1 0 0 51 51 48 3 0 3 1 0 0 1 0 1 0 0 0 0 0 0 30 24 17 7 0 7 2 1 0 1 0 0 0 0 1 0 1 1 117 114 103 11 3 8 4 2 2 0 1 0 0 0 1 1 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcagcctcacttcctatggc atgaactgggtccgccaggctccagggagggggctggagtgggtctcacac gttaatatgggtagtactcacata tactacgtaggctccgtgaggggccgattcaccatctccagagacgacgccaagaactcagtgtatctgcagatgaacaacttgagagccgaggacacggctctatattactgt gtgagagatagtggcccgtccggctacgctaggtacgagtctatggacgtc VRDSGPSGYARYESMDV IGHV3-21 IGHD6-6 IGHJ6 ggattcagcctcacttcctatggc atgaactgggtccgccaggctccagggagggggctggagtgggtctcacac gttaatatgggtagtactcacata tactacgtaggctccgtgaggggccgattcaccatctccagagacgacgccaagaactcagtgtatctgcagatgaacaacttgagagccgaggacacggctctatattactgt gtgagagatagtggcccgtccggctacgctaggtacgagtctatggacgtc IGG1 27 213 16 11 5 13 11 14 3 2 11 11 JY8QFUQ01BN76B 16332 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 851 88.07 192/218 nt 87.61 191/218 nt Homsap IGHJ6*02 F (see comment) 175 75.81 47/62 nt Homsap IGHD3-3*01 F 3 8 8 17 8.8.17 [1.17.38.11] CARILTLFAVVTSSGMDVW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*01 (shorter alignment but highest percentage of identity) in FR3-IMGT at codon 75 of V-REGION: 'C' (1 nucleotides at position 129 to 129 in the user submitted sequence), (cause frameshift); cctctggatttaccttcagtaattatgacatgaattgggtccgccaggctccagggaaggggctggagtgggtctcatccatcaccagtagtggtagttatgtctaccattcagactcaacgaagggcCcgattcaccatctccagagacaacgcccagaattcactatttctgcaaatgaacaacctgagacccgaggacacggctatatattactgtgcgaggatcctgacgctttttgcagtggttacctccagtgggatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [301] c84>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a112>g,S38>D(- - -)|g113>a,S38>D(- - -)|c120>t|t168>c|g170>c,S57>T(+ - +)|t171>c,S57>T(+ - +)|a184>g,S62>G(+ + -)|c192>t|a193>g,I65>V(+ - +)|a195>c,I65>V(+ - +)|t199>c,Y67>H(+ - -)|c201>t,Y67>H(+ - -)|g202>t,A68>S(- + -)|g211>a,V71>T(- - -)|t212>c,V71>T(- - -)|a250>c,K84>Q(+ - -)|c255>t|g261>a|a263>t,Y88>F(- + -)|g278>a,S93>N(- - -)|g286>c,A96>P(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| c84>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a112>g,S38>D(- - -)|g113>a,S38>D(- - -)| c120>t| t168>c|g170>c,S57>T(+ - +)|t171>c,S57>T(+ - +)|a184>g,S62>G(+ + -)|c192>t|a193>g,I65>V(+ - +)|a195>c,I65>V(+ - +)| t199>c,Y67>H(+ - -)|c201>t,Y67>H(+ - -)|g202>t,A68>S(- + -)|g211>a,V71>T(- - -)|t212>c,V71>T(- - -)|a250>c,K84>Q(+ - -)|c255>t|g261>a|a263>t,Y88>F(- + -)|g278>a,S93>N(- - -)|g286>c,A96>P(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| a318>g|g319>a|a320>t| 317 (320) 223 (226) 197 26 (29) 6 (9) 20 3 (4) 7 (8) 6 4 2 0 1 (2) 0 2 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 1 2 2 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 17 7 2 5 2 0 1 2 1 0 0 0 1 0 0 0 117 114 101 13 2 11 0 5 2 2 1 0 1 0 1 0 1 0 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggatttaccttcagtaattatgac atgaattgggtccgccaggctccagggaaggggctggagtgggtctcatcc atcaccagtagtggtagttatgtc taccattcagactcaacgaagggccgattcaccatctccagagacaacgcccagaattcactatttctgcaaatgaacaacctgagacccgaggacacggctatatattactgt gcgaggatcctgacgctttttgcagtggttacctccagtgggatggacgtc ARILTLFAVVTSSGMDV IGHV3-21 IGHD3-3 IGHJ6 ggatttaccttcagtaattatgac atgaattgggtccgccaggctccagggaaggggctggagtgggtctcatcc atcaccagtagtggtagttatgtc taccattcagactcaacgaagggccgattcaccatctccagagacaacgcccagaattcactatttctgcaaatgaacaacctgagacccgaggacacggctatatattactgt gcgaggatcctgacgctttttgcagtggttacctccagtgggatggacgtc IGA1 26 213 20 6 13 16 7 10 3 3 11 9 JY8QFUQ01BNHM6 8536 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 968 94.04 205/218 nt 92.66 202/218 nt Homsap IGHJ6*02 F (see comment) 194 83.64 46/55 nt Homsap IGHD1-1*01 F 3 7 8 10 7.8.10 [2.17.38.11] CAREVSDALDVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) in CDR2-IMGT at codon 57 of V-REGION: 'A' (1 nucleotides at position 86 to 86 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 72 of V-REGION: 'G' (1 nucleotides at position 125 to 125 in the user submitted sequence), (cause frameshift); in CDR1-IMGT, from codon 37 of V-REGION: 1 nucleotides (from position 26 in the user submitted sequence), (cause frameshift) gcctctggattcatctttgatgatttggcatgagatgggtccgccaagttccagggaaggggctacagtgggtctctggtattaaAttggaatggtggtaaaacaggttatgcagactctgtgaGggggccgattcatcatctccagagacaacgccaagaacgccctgtatctgcaaatgaacagtctcagagccgaggacacggccttatattactgtgcgagagaggtctcggacgccttggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctagtccagggcttcttccccca NA IGA1 93 95 [281] c86>t,T29>I(- - -)|c120>a,S40>R(- - -)|c134>t,A45>V(+ - +)|g150>a|g151>c,E51>Q(+ + -)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|t256>g,S86>A(- + -)|g282>c|g303>a|c307>t,H103>Y(+ - -)| c86>t,T29>I(- - -)| c120>a,S40>R(- - -)|c134>t,A45>V(+ - +)|g150>a|g151>c,E51>Q(+ + -)| g191>a,S64>K(- - -)|c192>a,S64>K(- - -)| a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|t256>g,S86>A(- + -)|g282>c|g303>a|c307>t,H103>Y(+ - -)| 320 226 213 13 3 10 1 3 4 0 0 2 0 0 2 0 0 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 22 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 47 4 1 3 0 1 1 0 0 1 0 0 1 0 0 0 30 24 22 2 0 2 0 1 0 0 0 1 0 0 0 0 0 0 117 114 108 6 2 4 1 1 2 0 0 0 0 0 1 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| gcctct ggattcatctttgatgatttggc atgagatgggtccgccaagttccagggaaggggctacagtgggtctctggt attaattggaatggtggtaaaaca ggttatgcagactctgtgaggggccgattcatcatctccagagacaacgccaagaacgccctgtatctgcaaatgaacagtctcagagccgaggacacggccttatattactgt gcgagagaggtctcggacgccttggacgtc AREVSDALDV IGHV3-20 IGHD1-1 IGHJ6 ggattcatctttgatgatttggc atgagatgggtccgccaagttccagggaaggggctacagtgggtctctggt attaattggaatggtggtaaaaca ggttatgcagactctgtgaggggccgattcatcatctccagagacaacgccaagaacgccctgtatctgcaaatgaacagtctcagagccgaggacacggccttatattactgt gcgagagaggtctcggacgccttggacgtc IGA1 13 212 8 5 7 11 1 2 3 0 7 3 JY8QFUQ01BNNYX 18635 productive (see comment) Homsap IGHV2-5*02 F 1116 95.49 233/244 nt 94.67 231/244 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-2*01 F 2 10 7 14 10.7.14 [9.17.38.11] CAHIRAELSTSCHAFW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 81 of V-REGION: 'G' (1 nucleotides at position 173 to 173 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 84 of V-REGION: 'A' (1 nucleotides at position 185 to 185 in the user submitted sequence), (cause frameshift); gaccctcacgctgacctgcaccttctctgggttctcactcaacactgttggaatgggtgtgggctgggtccgtcagcccccaggaaaggccctggagtggcttgcacttgtttattgggatgatgataagcgctacagctcatctctgaagagcagactcaccatcaccaagGgacacctccaaAaaaccaggtggtccttacaatgactaacatggaccctatggacacagccacatattactgtgcacacattagggcggaacttagtaccagctgccatgccttctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 89 [320, 319] g92>a,S31>N(- - -)|a103>g,S35>V(- - -)|g104>t,S35>V(- - -)|g109>a,V37>M(+ - -)|a124>g,I42>V(+ - +)|c165>t|a166>g,I56>V(+ - +)|c205>t,P69>S(+ - -)|g225>a|c276>t|g289>a,V97>M(+ - -)| g92>a,S31>N(- - -)|a103>g,S35>V(- - -)|g104>t,S35>V(- - -)|g109>a,V37>M(+ - -)| a124>g,I42>V(+ - +)|c165>t| a166>g,I56>V(+ - +)| c205>t,P69>S(+ - -)|g225>a|c276>t|g289>a,V97>M(+ - -)| g320>t,R107>I(- + -)|a321>t,R107>I(- + -)|c322>a| 319 (322) 251 (254) 240 11 (14) 3 (4) 8 (10) 3 4 3 0 0 0 (1) 0 (1) 0 0 0 1 (2) 0 78 28 28 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 0 4 1 2 0 0 0 0 0 0 0 0 1 0 51 51 49 2 1 1 1 0 1 0 0 0 0 0 0 0 0 0 30 21 20 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 117 114 110 4 2 2 0 2 2 0 0 0 0 0 0 0 0 0 7 (10) 7 7 0 (3) 0 (1) 0 (2) 0 0 0 0 0 0 (1) 0 (1) 0 0 0 0 (1) 0 aa,13-14(FR1)|aa,40-41(FR1)|ta,102-103(CDR1)|aa,141-142(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,238-239(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,267-268(FR3)|aa,270-271(FR3)|aa,277-278(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,10-11(FR1)|tt,73-74(FR1)|tt,82-83(CDR1)|ta,102-103(CDR1)|tt,158-159(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,80-83(CDR1)|ggct,118-121(FR2)|ggct,155-158(FR2)|agca,220-223(FR3)|ggct,224-227(FR3)| aacc,41-44(FR1)|agcc,131-134(FR2)|agcc,202-205(FR3)|aacc,253-256(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)| gaccctcacgctgacctgcaccttctct gggttctcactcaacactgttggaatgggt gtgggctgggtccgtcagcccccaggaaaggccctggagtggcttgcactt gtttattgggatgatgataag cgctacagctcatctctgaagagcagactcaccatcaccaaggacacctccaaaaaccaggtggtccttacaatgactaacatggaccctatggacacagccacatattactgt gcacacattagggcggaacttagtaccagctgccatgccttc AHIRAELSTSCHAF IGHV2-5 IGHD2-2 IGHJ4 gggttctcactcaacactgttggaatgggt gtgggctgggtccgtcagcccccaggaaaggccctggagtggcttgcactt gtttattgggatgatgataag cgctacagctcatctctgaagagcagactcaccatcaccaaggacacctccaaaaaccaggtggtccttacaatgactaacatggaccctatggacacagccacatattactgt gcacacattagggcggaacttagtaccagctgccatgccttc IGA1 11 216 10 1 7 8 3 3 3 0 3 5 JY8QFUQ01BNQ6N 13684 productive Homsap IGHV3-23*03 F (see comment) 894 91.12 195/214 nt NA Homsap IGHJ4*02 F 114 70.83 34/48 nt NA 8 7 8 8.7.8 [1.17.38.11] CARGAPFYDS (see Functionality comment) in-frame - IMGT/JunctionAnalysis gives no results for this JUNCTION The submitted sequence and the closest germline V-GENE allele show different CDR2-IMGT amino acid lengths (7 AA in JY8QFUQ01BNQ6N ; 8 AA in Homsap_IGHV3-23*03) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctggattcacctttggttattatggcatgagttgggtccgccaggctccagggaaggggctggactgcgtctcagttatttatcccggtggtagcacatactatgcagactccgtgaagggccgattcaccatctccagagacatctccaagaacacactgtatcttcaaatgaacagcctgagagccgaggacacggctgtatattactgtgcgagaggggccccattttatgatagtgttaagggaaccctggtcaccgtctcttcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG2 100 100 [270] a103>g,S35>G(+ + -)|c105>t,S35>G(+ + -)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c108>t,S36>Y(+ - -)|c113>g,A38>G(- + -)|c120>t|g153>c,E51>D(+ - +)|g156>c,W52>C(+ - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>g,S63>G(+ + -)|g225>a|t243>c|a245>t,N82>I(- - -)|t246>c,N82>I(- - -)|g258>a|g267>t|c300>t|a317>g,K106>R(+ + +)| a103>g,S35>G(+ + -)|c105>t,S35>G(+ + -)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c108>t,S36>Y(+ - -)|c113>g,A38>G(- + -)| c120>t|g153>c,E51>D(+ - +)|g156>c,W52>C(+ - -)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>g,S63>G(+ + -)| g225>a|t243>c|a245>t,N82>I(- - -)|t246>c,N82>I(- - -)|g258>a|g267>t|c300>t| a317>g,K106>R(+ + +)|a320>g| 319 (320) 221 (222) 201 20 (21) 6 (7) 14 3 (4) 3 4 2 1 0 2 0 3 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 1 2 0 0 0 1 0 0 1 0 0 51 51 48 3 1 2 0 0 1 0 0 0 0 0 2 0 0 0 30 21 18 3 0 3 1 0 0 0 1 0 0 0 1 0 0 0 117 114 107 7 5 2 0 2 1 2 0 0 1 0 0 0 1 0 7 (8) 7 6 1 (2) 0 (1) 1 1 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|ta,243-244(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,243-244(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,184-187(CDR2)|agca,190-193(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttggttattatggc atgagttgggtccgccaggctccagggaaggggctggactgcgtctcagtt atttatcccggtggtagcaca tactatgcagactccgtgaagggccgattcaccatctccagagacatctccaagaacacactgtatcttcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgagaggggccccattttatgat ARGAPFYD IGHV3-23 IGHJ4 ggattcacctttggttattatggc atgagttgggtccgccaggctccagggaaggggctggactgcgtctcagtt atttatcccggtggtagcaca tactatgcagactccgtgaagggccgattcaccatctccagagacatctccaagaacacactgtatcttcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgagaggggccccattttatgat IGG2 19 210 11 8 7 12 4 7 6 0 4 9 JY8QFUQ01BNS72 12516 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F (see comment) 890 90.7 195/215 nt NA Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD5-18*01 F 3 10 7 17 10.7.17 [1.17.38.11] CAGEQMIDAVSHNYAMDVW in-frame + The submitted sequence and the closest germline V-GENE allele show different CDR1-IMGT amino acid lengths (10 AA in JY8QFUQ01BNS72 ; 8 AA in Homsap_IGHV4-59*01) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tctctggtggctccatcagcatcaatacttacttctggagttggatccggcagcccccagggaagggactggagtggattgggtatatctctcacagtgggagtgccaactacaacccctccctcgagagtcgagtcaccatcttaagagacacgtccaagaaccagttctctctgaggctgagggctgtaaccgcggcggacacggccgtgtatttctgtgcgggggaacagatgattgacgcagtctcccacaactatgccatggacgtctggggccaagggaccacggtcatcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 79 95 [303, 302] g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)|a113>t,Y38>F(- + -)|c120>t|a170>c,Y57>S(+ - -)|t172>c,Y58>H(+ - -)|c192>t|a193>g,T65>A(- - -)|a214>g,K72>E(+ - -)|a234>c|c236>t,S79>L(- - -)|g238>a,V80>R(- - -)|t239>g,V80>R(- - -)|c264>t|a269>g,K90>R(+ + +)|c276>g,S92>R(- - -)|t277>g,S93>A(- + -)|g282>a|t288>g|a308>t,Y103>F(- + -)|a316>g,R106>G(- - -)|a318>g,R106>G(- - -)| g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)|a113>t,Y38>F(- + -)| c120>t| a170>c,Y57>S(+ - -)|t172>c,Y58>H(+ - -)|c192>t|a193>g,T65>A(- - -)| a214>g,K72>E(+ - -)|a234>c|c236>t,S79>L(- - -)|g238>a,V80>R(- - -)|t239>g,V80>R(- - -)|c264>t|a269>g,K90>R(+ + +)|c276>g,S92>R(- - -)|t277>g,S93>A(- + -)|g282>a|t288>g|a308>t,Y103>F(- + -)| a316>g,R106>G(- - -)|a318>g,R106>G(- - -)| 320 229 207 22 6 16 5 3 4 1 2 0 2 0 1 1 0 3 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 0 1 0 0 0 0 1 0 1 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 21 17 4 1 3 1 0 1 1 1 0 0 0 0 0 0 0 117 114 102 12 4 8 2 2 2 0 1 0 1 0 0 1 0 3 8 8 6 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcatcaatacttacttc tggagttggatccggcagcccccagggaagggactggagtggattgggtat atctctcacagtgggagtgcc aactacaacccctccctcgagagtcgagtcaccatcttaagagacacgtccaagaaccagttctctctgaggctgagggctgtaaccgcggcggacacggccgtgtatttctgt gcgggggaacagatgattgacgcagtctcccacaactatgccatggacgtc AGEQMIDAVSHNYAMDV IGHV4-59 IGHD5-18 IGHJ6 ggtggctccatcagcatcaatacttacttc tggagttggatccggcagcccccagggaagggactggagtggattgggtat atctctcacagtgggagtgcc aactacaacccctccctcgagagtcgagtcaccatcttaagagacacgtccaagaaccagttctctctgaggctgagggctgtaaccgcggcggacacggccgtgtatttctgt gcgggggaacagatgattgacgcagtctcccacaactatgccatggacgtc IGA2 20 216 11 9 7 9 4 11 5 1 8 6 JY8QFUQ01BNVKG 13520 productive (see comment) Homsap IGHV4-34*03 F (see comment) 760 83.72 180/215 nt 83.26 179/215 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-16*02 F 1 8 7 13 8.7.13 [2.17.38.11] CTRGRLDAWEVSDHW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (83,72% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR2-IMGT at codon 54 of V-REGION: 'A' (1 nucleotides at position 76 to 76 in the user submitted sequence), (cause frameshift); gtcgacgatgggtcctgtagagagtgcttctggagttggatccgccagtccccagggaagggtctggagtggattAggggacgtcaattatagacgaggcgtcgactacaacccgtccctcaagagtcgagtgaccatatcattggacacgtccaacaaacaagtctccctgagtctgagttctgtcaccgccgcggacacggccatgtattattgtacgcgagggcgattagacgcgtgggaagtatcggaccactggggccagggaaccgtggtcaccgtctcctcagcctccaccaaggggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 94 [289] t76>g,Y26>D(- - -)|t78>c,Y26>D(- - -)|g80>a,G27>D(- - -)|t89>g,F30>C(+ - -)|c90>t,F30>C(+ - -)|t105>a,S35>R(- - -)|g107>a,G36>E(- - -)|t108>g,G36>E(- - -)|a110>g,Y37>C(- - -)|a113>t,Y38>F(- + -)|c120>t|c133>t,P45>S(+ - -)|g147>t|a165>c,E55>D(+ - +)|a166>g,I56>V(+ - +)|c172>t,H58>Y(+ - -)|t177>a,S59>R(- - -)|g187>c,G63>R(- - -)|a190>g,S64>G(+ + -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|a196>g,N66>D(+ + -)|c228>g|g238>t,V80>L(+ - +)|a240>g,V80>L(+ - +)|g252>c,K84>N(+ - -)|c255>a,N85>K(+ - -)|g258>a|t259>g,F87>V(+ - -)|a269>g,K90>S(- - -)|g270>t,K90>S(- - -)|c276>t|g282>c|g301>a,V101>M(+ - -)|c309>t| t76>g,Y26>D(- - -)|t78>c,Y26>D(- - -)| g80>a,G27>D(- - -)|t89>g,F30>C(+ - -)|c90>t,F30>C(+ - -)|t105>a,S35>R(- - -)|g107>a,G36>E(- - -)|t108>g,G36>E(- - -)|a110>g,Y37>C(- - -)|a113>t,Y38>F(- + -)| c120>t|c133>t,P45>S(+ - -)|g147>t|a165>c,E55>D(+ - +)| a166>g,I56>V(+ - +)|c172>t,H58>Y(+ - -)|t177>a,S59>R(- - -)|g187>c,G63>R(- - -)|a190>g,S64>G(+ + -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)| a196>g,N66>D(+ + -)|c228>g|g238>t,V80>L(+ - +)|a240>g,V80>L(+ - +)|g252>c,K84>N(+ - -)|c255>a,N85>K(+ - -)|g258>a|t259>g,F87>V(+ - -)|a269>g,K90>S(- - -)|g270>t,K90>S(- - -)|c276>t|g282>c|g301>a,V101>M(+ - -)|c309>t| 311 215 180 35 7 28 7 4 7 1 1 1 1 2 3 1 3 4 78 6 4 2 0 2 0 0 0 1 0 0 0 0 0 0 0 1 36 24 16 8 0 8 1 2 1 0 0 0 1 1 0 0 0 2 51 51 47 4 2 2 0 0 2 0 1 0 0 0 0 0 1 0 30 21 14 7 0 7 3 0 2 0 0 0 0 1 1 0 0 0 116 114 99 14 5 9 3 2 2 0 0 1 0 0 2 1 2 1 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtcgac gatgggtcctgtagagagtgcttc tggagttggatccgccagtccccagggaagggtctggagtggattggggac gtcaattatagacgaggcgtc gactacaacccgtccctcaagagtcgagtgaccatatcattggacacgtccaacaaacaagtctccctgagtctgagttctgtcaccgccgcggacacggccatgtattattgt acgcgagggcgattagacgcgtgggaagtatcggaccac TRGRLDAWEVSDH IGHV4-34 IGHD3-16 IGHJ4 gatgggtcctgtagagagtgcttc tggagttggatccgccagtccccagggaagggtctggagtggattggggac gtcaattatagacgaggcgtc gactacaacccgtccctcaagagtcgagtgaccatatcattggacacgtccaacaaacaagtctccctgagtctgagttctgtcaccgccgcggacacggccatgtattattgt acgcgagggcgattagacgcgtgggaagtatcggaccac IGG2 33 210 18 15 11 19 7 14 7 0 11 15 JY8QFUQ01BNXKI 9011 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 874 89.04 195/219 nt 88.13 193/219 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [2.16.38.11] CVRAGFGELSGFDCW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'C' (1 nucleotides at position 17 to 17 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 41 of V-REGION: 1 nucleotides (from position 40 in the user submitted sequence), (cause frameshift) gcctcctggattcaccCtttggttattatggcatggactggtccgccaactccgggggagggggctggagtgggtctctggtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [291] a72>g|g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>P(+ - -)|a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a118>g,S40>D(- - -)|g119>a,S40>D(- - -)|g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|t135>c,A45>L(+ - +)|c137>g,P46>R(- - -)|a138>g,P46>R(- - -)|a143>g,K48>R(+ + +)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a72>g|g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>P(+ - -)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a118>g,S40>D(- - -)|g119>a,S40>D(- - -)|g133>c,A45>L(+ - +)|c134>t,A45>L(+ - +)|t135>c,A45>L(+ - +)|c137>g,P46>R(- - -)|a138>g,P46>R(- - -)|a143>g,K48>R(+ + +)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 226 (227) 201 25 (26) 3 (4) 22 5 4 3 3 1 (2) 0 2 0 4 2 1 0 78 7 3 4 1 3 1 0 1 1 0 0 0 0 1 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 50 42 8 0 8 3 1 1 1 0 0 0 0 1 1 0 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| gcctcct ggattcacctttggttattatggc atggactggtccgccaactccgggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atggactggtccgccaactccgggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 20 212 11 9 5 11 6 9 2 0 14 4 JY8QFUQ01BNYDH 12044 productive (see comment) Homsap IGHV4-61*02 F 969 93.64 206/220 nt 92.73 204/220 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD2-21*02 F 3 9 7 15 9.7.15 [1.17.38.11] CARGRTVVTVVDYVDFW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 39 of V-REGION: 'C' (1 nucleotides at position 35 to 35 in the user submitted sequence), (cause frameshift); in CDR1-IMGT, from codon 28 of V-REGION: 1 nucleotides (from position 10 in the user submitted sequence), (cause frameshift) tctctggtgctccatcagcagtggtagtttctacCtggagctggatccggcagcccgccgggaagggactggagtggatggggcgtgtctatgccagtggaaccaccaagttcaacccctccctcaagagtcgagtcactttatcagtagacacgtccaagaaccagttctccctgaaactgacctctgtgaccgctgcggacacggccgtgtattactgtgcgagaggacgaacggtggtgactgttgttgactacgttgacttttggggtcagggagtcctggtcaacgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] a110>t,Y37>F(- + -)|t159>g,I53>M(+ + -)|a166>g,I56>V(+ - +)|a172>g,T58>A(- - -)|g189>a|g191>c,S64>T(+ - +)|c198>g,N66>K(+ - -)|a200>t,Y67>F(- + -)|c231>t|a232>t,I78>L(+ + +)|g270>a|g275>c,S92>T(+ - +)|c288>t|a291>g| a110>t,Y37>F(- + -)| t159>g,I53>M(+ + -)| a166>g,I56>V(+ - +)|a172>g,T58>A(- - -)|g189>a|g191>c,S64>T(+ - +)| c198>g,N66>K(+ - -)|a200>t,Y67>F(- + -)|c231>t|a232>t,I78>L(+ + +)|g270>a|g275>c,S92>T(+ - +)|c288>t|a291>g| a320>g| 319 (320) 227 (228) 213 14 (15) 5 (6) 9 3 (4) 2 2 0 0 0 3 0 2 1 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 29 28 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 30 21 17 4 1 3 2 1 0 0 0 0 0 0 1 0 0 0 117 114 106 8 4 4 1 1 2 0 0 0 2 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgctccatcagcagtggtagtttctac tggagctggatccggcagcccgccgggaagggactggagtggatggggcgt gtctatgccagtggaaccacc aagttcaacccctccctcaagagtcgagtcactttatcagtagacacgtccaagaaccagttctccctgaaactgacctctgtgaccgctgcggacacggccgtgtattactgt gcgagaggacgaacggtggtgactgttgttgactacgttgacttt ARGRTVVTVVDYVDF IGHV4-61 IGHD2-21 IGHJ4 ggtgctccatcagcagtggtagtttctac tggagctggatccggcagcccgccgggaagggactggagtggatggggcgt gtctatgccagtggaaccacc aagttcaacccctccctcaagagtcgagtcactttatcagtagacacgtccaagaaccagttctccctgaaactgacctctgtgaccgctgcggacacggccgtgtattactgt gcgagaggacgaacggtggtgactgttgttgactacgttgacttt IGA1 14 215 7 7 4 7 3 7 4 1 5 4 JY8QFUQ01BO1TD 15590 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 859 88.89 192/216 nt NA Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F (see comment) 186 87.5 42/48 nt Homsap IGHD6-6*01 F 2 8 8 15 8.8.15 [1.17.38.11] CAKGDSPIASRLYIDSW in-frame + Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) tctggattcatttttagcaattatgccatgaactgggtccgccaggctccagggaaggggccggagtgggtctcagcttttagtggtggtggcactaagacctactacgcagactccgtgaagggccggttcttcatctccagagacaattccaagaacactctacatctgcatatgagcagcctgagggccgaggacacggccacatattactgtgcgaaaggggactcgcctatagcgtctcgtctatatattgactcctggggccaggggaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [293] c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g119>a,S40>N(- - -)|t149>c,L50>P(- - -)|a166>t,I56>F(+ - -)|a175>g,S59>G(+ + -)|t186>c|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|g191>a,S64>K(- - -)|c192>g,S64>K(- - -)|a195>c|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|g258>t|g261>a|t262>c,Y88>H(+ - -)|a270>t,Q90>H(- + -)|a275>g,N92>S(- - -)|a285>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g119>a,S40>N(- - -)|t149>c,L50>P(- - -)| a166>t,I56>F(+ - -)|a175>g,S59>G(+ + -)|t186>c|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|g191>a,S64>K(- - -)|c192>g,S64>K(- - -)|a195>c| a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|g258>t|g261>a|t262>c,Y88>H(+ - -)|a270>t,Q90>H(- + -)|a275>g,N92>S(- - -)|a285>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)| a320>g| 319 (320) 223 (224) 199 24 (25) 5 (6) 19 3 (4) 6 4 4 1 0 3 0 1 1 1 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 3 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 30 24 16 8 2 6 1 2 0 1 1 0 1 0 1 1 0 0 117 114 104 10 3 7 2 2 1 2 0 0 2 0 0 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcatttttagcaattatgcc atgaactgggtccgccaggctccagggaaggggccggagtgggtctcagct tttagtggtggtggcactaagacc tactacgcagactccgtgaagggccggttcttcatctccagagacaattccaagaacactctacatctgcatatgagcagcctgagggccgaggacacggccacatattactgt gcgaaaggggactcgcctatagcgtctcgtctatatattgactcc AKGDSPIASRLYIDS IGHV3-23 IGHD6-6 IGHJ4 ggattcatttttagcaattatgcc atgaactgggtccgccaggctccagggaaggggccggagtgggtctcagct tttagtggtggtggcactaagacc tactacgcagactccgtgaagggccggttcttcatctccagagacaattccaagaacactctacatctgcatatgagcagcctgagggccgaggacacggccacatattactgt gcgaaaggggactcgcctatagcgtctcgtctatatattgactcc IGG1 24 213 17 7 10 13 7 11 3 2 9 10 JY8QFUQ01BO2JV 14493 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD4-17*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAGHLLGDPFDYW in-frame + ttctggagacaactttagcagatactggatcggctgggtccgccagatgcccgggaaaggcctggagtggatggggatcatctatcctggtgactctgacaccagatacagtccgtccttccaaggccaggtcaccatctcagccgacaagtccaccagtaccgtctacctgcagtggagcagtctgaaggtctcggacaccgccacgtattactgtgcgggacatcttctcggtgacccttttgactactggggccagggaaccccggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccccctggcaccctcctccaaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggcaactcag NA IGG1 79 88 [282, 283, 284] t82>g,Y28>D(- - -)|g86>a,S29>N(- - -)|c104>g,T35>S(+ - +)|c108>a,S36>R(- - -)|g126>c|t192>c|c204>t|t251>c,I84>T(- - -)|c255>t|c260>t,A87>V(+ - +)|c279>t|c287>t,A96>V(+ - +)|t302>c,M101>T(- - -)|a316>g,R106>G(- - -)| t82>g,Y28>D(- - -)|g86>a,S29>N(- - -)|c104>g,T35>S(+ - +)|c108>a,S36>R(- - -)| g126>c| t192>c| c204>t|t251>c,I84>T(- - -)|c255>t|c260>t,A87>V(+ - +)|c279>t|c287>t,A96>V(+ - +)|t302>c,M101>T(- - -)| a316>g,R106>G(- - -)| 320 225 211 14 5 9 1 1 5 3 0 1 0 0 1 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 0 0 0 1 0 0 0 1 0 1 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 107 7 3 4 0 0 5 2 0 0 0 0 0 0 0 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ttct ggagacaactttagcagatactgg atcggctgggtccgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgacacc agatacagtccgtccttccaaggccaggtcaccatctcagccgacaagtccaccagtaccgtctacctgcagtggagcagtctgaaggtctcggacaccgccacgtattactgt gcgggacatcttctcggtgacccttttgactac AGHLLGDPFDY IGHV5-51 IGHD4-17 IGHJ4 ggagacaactttagcagatactgg atcggctgggtccgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgacacc agatacagtccgtccttccaaggccaggtcaccatctcagccgacaagtccaccagtaccgtctacctgcagtggagcagtctgaaggtctcggacaccgccacgtattactgt gcgggacatcttctcggtgacccttttgactac IGG1 13 213 9 4 6 9 3 4 4 1 4 4 JY8QFUQ01BO2V4 12198 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23*05 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 833 87.16 190/218 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 2 8 8 10 8.8.10 [1.17.38.11] CATWIQSHFDYW in-frame + cctctggattcacatttagtcactataccatgaattgggtccgccaggctccagggaaggggctggagtgggtctcagccattaatcaagatgccattaccacacactacccagactccgtgaagggccgcttcaccgtctccagagacaattccaagaacacactctatctgcaaatgagcagcctgagagccgacgacacggccgtatattattgtgcgacgtggatacagtctcactttgactattggggccagggaaccgtggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [279] c87>a|c105>t|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|g112>a,A38>T(- - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|t165>c|g170>a,S57>N(- - -)|g172>c,G58>Q(- - -)|g173>a,G58>Q(- - -)|t174>a,G58>Q(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g185>c,G62>A(- + -)|t186>c,G62>A(- + -)|g187>a,G63>I(- - -)|g188>t,G63>I(- - -)|g191>c,S64>T(+ - +)|t196>c,Y66>H(+ - -)|g202>c,A68>P(- - -)|g225>c|a232>g,I78>V(+ - +)|g258>a|g261>c|a275>g,N92>S(- - -)|g291>c,E97>D(+ - +)|c309>t| c87>a|c105>t|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|g112>a,A38>T(- - -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|t165>c| g170>a,S57>N(- - -)|g172>c,G58>Q(- - -)|g173>a,G58>Q(- - -)|t174>a,G58>Q(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g185>c,G62>A(- + -)|t186>c,G62>A(- + -)|g187>a,G63>I(- - -)|g188>t,G63>I(- - -)|g191>c,S64>T(+ - +)| t196>c,Y66>H(+ - -)|g202>c,A68>P(- - -)|g225>c|a232>g,I78>V(+ - +)|g258>a|g261>c|a275>g,N92>S(- - -)|g291>c,E97>D(+ - +)|c309>t| a317>c,K106>T(- - -)|a318>g,K106>T(- - -)|g319>t|a320>g| 316 (320) 222 (226) 194 28 (32) 7 (9) 21 (23) 3 (5) 8 3 3 1 (2) 1 0 1 7 0 1 (2) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 2 1 0 1 1 0 0 0 0 0 0 51 51 48 3 1 2 0 1 1 1 0 0 0 0 0 0 0 0 30 24 13 11 0 11 1 4 0 1 0 0 0 1 3 0 1 0 117 114 105 9 4 5 2 1 1 1 0 0 0 0 4 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (2) 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacatttagtcactatacc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagcc attaatcaagatgccattaccaca cactacccagactccgtgaagggccgcttcaccgtctccagagacaattccaagaacacactctatctgcaaatgagcagcctgagagccgacgacacggccgtatattattgt gcgacgtggatacagtctcactttgactat ATWIQSHFDY IGHV3-23 IGHD5-18 IGHJ4 ggattcacatttagtcactatacc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagcc attaatcaagatgccattaccaca cactacccagactccgtgaagggccgcttcaccgtctccagagacaattccaagaacacactctatctgcaaatgagcagcctgagagccgacgacacggccgtatattattgt gcgacgtggatacagtctcactttgactat IGA1 28 213 17 11 11 20 6 8 5 2 7 14 JY8QFUQ01BO4ET 18718 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-8*01 F 2 8 8 8 8.8.8 [1.17.38.11] CARDNYGDYW in-frame + ctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgtgttaatagtgatgggggtaggacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcacagtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 89 [272, 271] g153>t|a166>g,I56>V(+ - +)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g153>t| a166>g,I56>V(+ - +)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 225 213 12 5 7 3 2 0 0 0 0 0 1 3 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 30 24 21 3 0 3 2 0 0 0 0 0 0 0 0 1 0 0 117 114 106 8 4 4 1 2 0 0 0 0 0 1 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgt gttaatagtgatgggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgt gttaatagtgatgggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac IGA2 12 213 5 7 2 8 3 4 5 0 4 3 JY8QFUQ01BO7P5 6759 productive (see comment) Homsap IGHV4-61*02 F 815 86.24 188/218 nt 85.78 187/218 nt Homsap IGHJ4*02 F (see comment) 168 83.33 40/48 nt Homsap IGHD3-3*01 F 3 9 7 16 9.7.16 [1.17.38.11] CARERSASIGGAGEIDSW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT, from codon 37 of V-REGION: 3 nucleotides (from position 30 in the user submitted sequence), (do not cause frameshift) tctctggcggccccatcagtggtggtggttactggagttggatccggcagcccgccgggaagggactggagtggatcggacatatttataatagtgggaacatcaactacagtccctccctcaagagtcgggttttcatgtcagtagacacctctaagaagcagttttccctgaggttgaactctgtgaccgccgcggacacggccgtgtattattgtgcgagagaacgtagtgcttcgattggcggagcgggagagattgactcctggggccagggaatcctggtcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] t81>c|t85>c,S29>P(+ - -)|c93>t|a100>g,S34>G(+ + -)|a106>g,S36>G(+ + -)|c120>t|t159>c|g162>a|g164>a,R55>H(- - +)|c168>t|c173>a,T58>N(- + -)|c174>t,T58>N(- + -)|g191>a,S64>N(- - -)|c194>t,T65>I(- - -)|a203>g,N68>S(- - -)|c204>t,N68>S(- - -)|a225>g|c228>t|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|a234>g,I78>M(+ + -)|g246>c|c249>t|c255>g,N85>K(+ - -)|c261>t|a269>g,K90>R(+ + +)|c271>t|g275>a,S92>N(- - -)|a291>g|c309>t| t81>c|t85>c,S29>P(+ - -)|c93>t|a100>g,S34>G(+ + -)|a106>g,S36>G(+ + -)| c120>t|t159>c|g162>a|g164>a,R55>H(- - +)| c168>t|c173>a,T58>N(- + -)|c174>t,T58>N(- + -)|g191>a,S64>N(- - -)|c194>t,T65>I(- - -)| a203>g,N68>S(- - -)|c204>t,N68>S(- - -)|a225>g|c228>t|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|a234>g,I78>M(+ + -)|g246>c|c249>t|c255>g,N85>K(+ - -)|c261>t|a269>g,K90>R(+ + +)|c271>t|g275>a,S92>N(- - -)|a291>g|c309>t| 320 226 196 30 14 16 7 4 12 3 0 1 1 0 1 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 27 22 5 2 3 2 0 1 2 0 0 0 0 0 0 0 0 51 51 47 4 3 1 0 2 1 1 0 0 0 0 0 0 0 0 30 21 16 5 1 4 0 1 3 0 0 1 0 0 0 0 0 0 117 114 98 16 8 8 5 1 7 0 0 0 1 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggcggccccatcagtggtggtggttac tggagttggatccggcagcccgccgggaagggactggagtggatcggacat atttataatagtgggaacatc aactacagtccctccctcaagagtcgggttttcatgtcagtagacacctctaagaagcagttttccctgaggttgaactctgtgaccgccgcggacacggccgtgtattattgt gcgagagaacgtagtgcttcgattggcggagcgggagagattgactcc ARERSASIGGAGEIDS IGHV4-61 IGHD3-3 IGHJ4 ggcggccccatcagtggtggtggttac tggagttggatccggcagcccgccgggaagggactggagtggatcggacat atttataatagtgggaacatc aactacagtccctccctcaagagtcgggttttcatgtcagtagacacctctaagaagcagttttccctgaggttgaactctgtgaccgccgcggacacggccgtgtattattgt gcgagagaacgtagtgcttcgattggcggagcgggagagattgactcc IGA1 30 213 26 4 16 19 10 11 11 3 9 7 JY8QFUQ01BO8WN 18668 productive (see comment) Homsap IGHV4-61*02 F 830 86.43 191/221 nt 85.52 189/221 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-18*01 F 3 10 7 15 10.7.15 [1.17.38.11] CARDTGQTYGRGPLDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 39 of V-REGION: 'G' (1 nucleotides at position 38 to 38 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 47 of V-REGION: 'G' (1 nucleotides at position 62 to 62 in the user submitted sequence), (cause frameshift); tctctggtgcctccataaataggggtaattattactgGgaactggatccggcagcccgccgGggaagggcctggaatggattggccgaatctatacaagtgggagcaccatctacaacccctccctcgggggtcgagtcaccatgactgtagacccgtccgagaatcagttcttcctgagactgagttctgtgaccgccgcagacacggccgtttatttttgtgcgagagacactggacaaacctacggtcggggccctttggactactggggccagggaatcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [300] g83>c,G28>A(- + -)|c90>a|g92>a,S31>N(- - -)|c93>t,S31>N(- - -)|t102>g,S34>R(- - -)|g107>a,S36>N(- - -)|c111>t|g119>a,S40>N(- - -)|a147>c|g153>a|g162>c|t165>a|c174>a|a197>t,N66>I(- - -)|a214>g,K72>G(- - -)|a215>g,K72>G(- - -)|a220>g,S74>G(+ + -)|a234>g,I78>M(+ + -)|t235>a,S79>T(+ - +)|a237>t,S79>T(+ - +)|a244>c,T82>P(+ + -)|a250>g,K84>E(+ - -)|c255>t|c263>t,S88>F(- - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c276>t|g303>t|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g83>c,G28>A(- + -)|c90>a|g92>a,S31>N(- - -)|c93>t,S31>N(- - -)|t102>g,S34>R(- - -)|g107>a,S36>N(- - -)|c111>t| g119>a,S40>N(- - -)|a147>c|g153>a|g162>c|t165>a| c174>a| a197>t,N66>I(- - -)|a214>g,K72>G(- - -)|a215>g,K72>G(- - -)|a220>g,S74>G(+ + -)|a234>g,I78>M(+ + -)|t235>a,S79>T(+ - +)|a237>t,S79>T(+ - +)|a244>c,T82>P(+ + -)|a250>g,K84>E(+ - -)|c255>t|c263>t,S88>F(- - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c276>t|g303>t|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| 320 229 199 30 10 20 6 5 6 0 2 2 3 2 2 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 23 7 2 5 0 2 2 0 0 1 0 0 1 0 0 1 51 51 46 5 4 1 0 2 0 0 1 0 0 1 1 0 0 0 30 21 20 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 117 114 97 17 3 14 6 1 4 0 1 0 3 1 0 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgcctccataaataggggtaattattac tggaactggatccggcagcccgccgggaagggcctggaatggattggccga atctatacaagtgggagcacc atctacaacccctccctcgggggtcgagtcaccatgactgtagacccgtccgagaatcagttcttcctgagactgagttctgtgaccgccgcagacacggccgtttatttttgt gcgagagacactggacaaacctacggtcggggccctttggactac ARDTGQTYGRGPLDY IGHV4-61 IGHD5-18 IGHJ4 ggtgcctccataaataggggtaattattac tggaactggatccggcagcccgccgggaagggcctggaatggattggccga atctatacaagtgggagcacc atctacaacccctccctcgggggtcgagtcaccatgactgtagacccgtccgagaatcagttcttcctgagactgagttctgtgaccgccgcagacacggccgtttatttttgt gcgagagacactggacaaacctacggtcggggccctttggactac IGG1 30 216 17 13 11 16 6 14 7 3 15 5 JY8QFUQ01BOBY3 21939 productive (see comment) Homsap IGHV4-4*02 F, or Homsap IGHV4-4*03 F or Homsap IGHV4-4*04 F or Homsap IGHV4-4*05 F 887 89.91 196/218 nt 89.45 195/218 nt Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD3-10*01 F 2 9 7 12 9.7.12 [1.17.38.11] CAANLGHGSYFKYW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 94 of V-REGION: 'T' (1 nucleotides at position 186 to 186 in the user submitted sequence), (cause frameshift); tctctggtgactccgtcagcagtgataactggtggagttgggtccgccagaccccagggaaggggctggagtggattggagaaatctatcatggtgggaccaccaactacaatccgtccctcaagggtcgagtcaccttatcggtcgacaagtccaagaaccaattctctctgagaatgacctctTtttaccgccgcagacacggccgtgtattactgtgcggccaatctgggtcatgggagttatttcaaatattggggcccgggaatcctggtcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [286] g83>a,G28>D(- - -)|a88>g,I30>V(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c133>a,P45>T(+ + -)|g162>a|a175>g,S59>G(+ + -)|g191>c,S64>T(+ - +)|c204>t|a220>g,S74>G(+ + -)|a232>t,I78>L(+ + +)|a237>g|a240>c|g258>a|c264>t|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c271>a,L91>M(+ + -)|g275>c,S92>T(+ - +)|g280>t,V94>F(+ - -)|g282>t,V94>F(+ - -)|g291>a| g83>a,G28>D(- - -)|a88>g,I30>V(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| c133>a,P45>T(+ + -)|g162>a| a175>g,S59>G(+ + -)|g191>c,S64>T(+ - +)| c204>t|a220>g,S74>G(+ + -)|a232>t,I78>L(+ + +)|a237>g|a240>c|g258>a|c264>t|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c271>a,L91>M(+ + -)|g275>c,S92>T(+ - +)|g280>t,V94>F(+ - -)|g282>t,V94>F(+ - -)|g291>a| a316>g,R106>A(- - -)|g317>c,R106>A(- - -)|a318>c,R106>A(- - -)|g319>a| 315 (320) 221 (226) 199 (200) 22 (26) 7 (8) 15 (18) 6 (7) 6 (7) 2 0 1 (2) 2 1 0 2 (3) 0 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 27 23 4 0 4 2 2 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 1 0 0 0 1 0 0 0 0 0 0 30 21 19 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 117 114 100 14 6 8 3 3 2 0 1 1 1 0 1 0 2 0 3 (8) 3 3 (4) 0 (4) 0 (1) 0 (3) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccgtcagcagtgataactgg tggagttgggtccgccagaccccagggaaggggctggagtggattggagaa atctatcatggtgggaccacc aactacaatccgtccctcaagggtcgagtcaccttatcggtcgacaagtccaagaaccaattctctctgagaatgacctcttttaccgccgcagacacggccgtgtattactgt gcggccaatctgggtcatgggagttatttcaaatat AANLGHGSYFKY IGHV4-4 IGHD3-10 IGHJ4 ggtgactccgtcagcagtgataactgg tggagttgggtccgccagaccccagggaaggggctggagtggattggagaa atctatcatggtgggaccacc aactacaatccgtccctcaagggtcgagtcaccttatcggtcgacaagtccaagaaccaattctctctgagaatgacctcttttaccgccgcagacacggccgtgtattactgt gcggccaatctgggtcatgggagttatttcaaatat IGA1 22 213 14 8 8 14 6 8 7 0 9 6 JY8QFUQ01BOMV5 16477 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 882 89.86 195/217 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [1.17.38.11] CTRDIGGRYSYW in-frame + ctccggattcacctttagtagtttctggatgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacggactaatgagtatgggagtatcacaaactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgtacaagggacatcggtgggcgctactcctactggggccagggagtcctggtctccgtctcatcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccgag NA IGA1 100 100 [278] t78>c|c90>t|c108>t|a110>t,Y37>F(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g247>a,A83>T(- - -)|c257>g,T86>R(- - -)|g261>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|g313>a,A105>T(- - -)|a318>g| t78>c| c90>t|c108>t|a110>t,Y37>F(- + -)| c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g247>a,A83>T(- - -)|c257>g,T86>R(- - -)|g261>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| g313>a,A105>T(- - -)|a318>g| 320 225 201 24 7 17 4 7 3 2 0 0 1 0 0 1 3 3 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 2 0 0 0 1 0 0 0 0 0 51 51 46 5 2 3 1 1 1 0 0 0 0 0 0 0 0 2 30 24 18 6 0 6 1 1 0 1 0 0 0 0 0 0 2 1 117 114 107 7 1 6 1 4 0 0 0 0 0 0 0 1 1 0 8 8 6 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcacctttagtagtttctgg atgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgt acaagggacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttagtagtttctgg atgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgt acaagggacatcggtgggcgctactcctac IGA1 21 213 13 8 9 13 4 8 3 2 9 7 JY8QFUQ01BP3RA 15418 productive Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F 953 93.95 202/215 nt NA Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD2-2*01 F 2 8 7 12 8.7.12 [1.17.38.11] CAVSTTTWGWFDPW in-frame - tctatggtgggtccttcagtggttactactggagctggatccgccagcccccaggggaggggctgcagtggattggaagagtcaatcatagtggaagcaccagctacaacccgtccctcaagagtcgagtcaccatgtcactagacacgtccaagacccacttttccctgaagctgacctctgtgaccgccgcggacacggctgtgtattactgtgcggtgtctactaccacctgggggtggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [282] a142>g,K48>E(+ - -)|g151>c,E51>Q(+ + -)|g162>a|g163>a,E55>R(+ - -)|a164>g,E55>R(+ - -)|a166>g,I56>V(+ - +)|a197>g,N66>S(- - -)|a234>g,I78>M(+ + -)|g238>c,V80>L(+ - +)|a254>c,N85>T(- + -)|g258>c,Q86>H(- + -)|c261>t|g275>c,S92>T(+ - +)| a142>g,K48>E(+ - -)|g151>c,E51>Q(+ + -)|g162>a|g163>a,E55>R(+ - -)|a164>g,E55>R(+ - -)| a166>g,I56>V(+ - +)| a197>g,N66>S(- - -)|a234>g,I78>M(+ + -)|g238>c,V80>L(+ - +)|a254>c,N85>T(- + -)|g258>c,Q86>H(- + -)|c261>t|g275>c,S92>T(+ - +)| a316>g,R106>V(- - -)|g317>t,R106>V(- - -)|a318>g,R106>V(- - -)|g319>t|g320>c| 315 (320) 218 (223) 205 13 (18) 2 (4) 11 (14) 5 (7) 2 1 0 1 0 0 0 4 (5) 0 0 (2) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 46 5 1 4 2 2 0 0 0 0 0 0 1 0 0 0 30 21 20 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 117 114 107 7 1 6 2 0 1 0 1 0 0 0 3 0 0 0 3 (8) 3 3 0 (5) 0 (2) 0 (3) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (2) 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctat ggtgggtccttcagtggttactac tggagctggatccgccagcccccaggggaggggctgcagtggattggaaga gtcaatcatagtggaagcacc agctacaacccgtccctcaagagtcgagtcaccatgtcactagacacgtccaagacccacttttccctgaagctgacctctgtgaccgccgcggacacggctgtgtattactgt gcggtgtctactaccacctgggggtggttcgacccc AVSTTTWGWFDP IGHV4-34 IGHD2-2 IGHJ5 ggtgggtccttcagtggttactac tggagctggatccgccagcccccaggggaggggctgcagtggattggaaga gtcaatcatagtggaagcacc agctacaacccgtccctcaagagtcgagtcaccatgtcactagacacgtccaagacccacttttccctgaagctgacctctgtgaccgccgcggacacggctgtgtattactgt gcggtgtctactaccacctgggggtggttcgacccc IGA1 13 210 8 5 3 7 5 6 2 0 10 1 JY8QFUQ01BP55P 13367 productive (see comment) Homsap IGHV3-7*01 F 922 92.13 199/216 nt 91.67 198/216 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-3*02 F 2 7 8 14 7.8.14 [1.17.38.11] CARGSYWHYDTFGDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 35 of V-REGION: 1 nucleotides (from position 18 in the user submitted sequence), (cause frameshift) ctctggatttacttttacaactattggatgacctgggttcgccaggctccagggaaggggctggaatgggtggccaacataaaacaacatggaggtgaaacgtactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgtgcgagagggtcgtattggcattatgataccttcggcgactattggggcccgggagtcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [290] c84>t|c87>t|t105>c|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|c126>t|g153>a|g171>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| c84>t|c87>t|t105>c|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|c126>t|g153>a| g171>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)| a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| a320>g| 319 (320) 223 (224) 206 17 (18) 8 (9) 9 3 (4) 4 3 1 2 0 0 0 3 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 19 4 3 1 0 1 2 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 0 1 0 0 0 30 24 18 6 2 4 2 2 0 0 1 0 0 0 1 0 0 0 117 114 110 4 1 3 1 0 0 0 1 0 0 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatttacttttacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccaac ataaaacaacatggaggtgaaacg tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat ARGSYWHYDTFGDY IGHV3-7 IGHD3-3 IGHJ4 ggatttacttttacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccaac ataaaacaacatggaggtgaaacg tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat IGG1 17 212 11 6 7 11 4 6 3 5 4 5 JY8QFUQ01BPG8K 19600 productive (see comment) Homsap IGHV4-61*02 F 885 90.65 194/214 nt 89.72 192/214 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-19*01 F 1 8 7 13 8.7.13 [1.17.38.11] CARQGDSSGPWNDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'CTGGTT' (6 nucleotides at position 21 to 26 in the user submitted sequence), (do not cause frameshift);in CDR2-IMGT at codon 64 of V-REGION: 'A' (1 nucleotides at position 103 to 103 in the user submitted sequence), (cause frameshift); ctccggtggctccatcagtaCTGGTTattactactggagctggatccggcagtccgccgggaagggactggaatggattgggcgcatgtctgccagagggggAcagcaactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgtgcgagacaaggggatagtagtggaccctggaatgactactggggccagggaatcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [292] t78>c|g103>a,G35>S(+ + -)|g107>a,S36>N(- - -)|c133>t,P45>S(+ - -)|g153>a|t165>c|c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>G(+ + -)|c194>g,T65>S(+ - +)|a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| t78>c| g103>a,G35>S(+ + -)|g107>a,S36>N(- - -)| c133>t,P45>S(+ - -)|g153>a|t165>c| c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>G(+ + -)|c194>g,T65>S(+ - +)| a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g319>c| 318 (320) 220 (222) 200 (201) 20 (21) 4 (5) 16 3 4 2 2 3 0 1 1 1 (2) 3 0 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 2 0 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 1 0 0 0 0 0 0 0 0 30 21 15 6 0 6 2 0 0 0 1 0 0 1 0 2 0 0 117 114 106 8 1 7 1 1 1 0 2 0 1 0 1 1 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctcc ggtggctccatcagtaattactac tggagctggatccggcagtccgccgggaagggactggaatggattgggcgc atgtctgccagagggggcagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac ARQGDSSGPWNDY IGHV4-61 IGHD6-19 IGHJ4 ggtggctccatcagtaattactac tggagctggatccggcagtccgccgggaagggactggaatggattgggcgc atgtctgccagagggggcagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac IGG2 19 210 10 9 6 10 4 9 3 0 8 8 JY8QFUQ01BPT7N 16323 productive Homsap IGHV3-7*01 F 1017 96.77 210/217 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD1-26*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARNTPAYWELSGDW in-frame + ctctggattcacctttaggagttattggatcagctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaacccagatggaagtgagaaatactatgtggactctgtgaggggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatggacagcctgagagccgaggacacggctgtgtattactgtgcgcgaaatacaccggcgtattgggaactatcaggtgactggggccagggaaccctggtcatcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccctaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 71 95 [287, 289] t105>g,S35>R(- - -)|c108>t|g117>c,M39>I(+ + -)|g171>c,K57>N(+ - -)|a173>c,Q58>P(- - -)|a215>g,K72>R(+ + +)|a274>g,N92>D(+ + -)|a316>c| t105>g,S35>R(- - -)|c108>t| g117>c,M39>I(+ + -)| g171>c,K57>N(+ - -)|a173>c,Q58>P(- - -)| a215>g,K72>R(+ + +)|a274>g,N92>D(+ + -)| a316>c|g319>a| 318 (320) 223 (225) 215 (216) 8 (9) 2 (3) 6 2 0 (1) 1 0 2 0 0 0 2 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 1 0 0 0 0 0 0 0 0 1 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 22 2 0 2 0 0 0 0 1 0 0 0 1 0 0 0 117 114 112 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 6 (8) 6 5 (6) 1 (2) 1 (2) 0 0 0 (1) 0 0 1 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaggagttattgg atcagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaacccagatggaagtgagaaa tactatgtggactctgtgaggggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatggacagcctgagagccgaggacacggctgtgtattactgt gcgcgaaatacaccggcgtattgggaactatcaggtgac ARNTPAYWELSGD IGHV3-7 IGHD1-26 IGHJ4 ggattcacctttaggagttattgg atcagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaacccagatggaagtgagaaa tactatgtggactctgtgaggggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatggacagcctgagagccgaggacacggctgtgtattactgt gcgcgaaatacaccggcgtattgggaactatcaggtgac IGA2 7 213 3 4 1 3 2 4 0 1 3 3 JY8QFUQ01BQ0GT 16098 productive (see comment) Homsap IGHV3-9*02 F 833 87.16 190/218 nt 86.7 189/218 nt Homsap IGHJ2*01 F (see comment) 157 77.36 41/53 nt Homsap IGHD4-11*01 ORF 3 8 8 17 8.8.17 [1.17.38.11] CARAPSVTFSGQGHYYDLW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*01 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 47 of V-REGION: 'C' (1 nucleotides at position 54 to 54 in the user submitted sequence), (cause frameshift); gcactggattcagctctgagaattatgccatgcactgggtccggcagcctccaCgggaagggcctagagtgggtctcacacatcagttggaatggcgaaggcatcgactacgcggactcagtgaagggccgattcaccgtctccagagacaacggcaagaattccctgtatttgcaaatgaacagtctgacaactgacgacacggccttgtattactgtgcaagagccccttcagtcacattcagcgggcaggggcactactatgatctctggggccgtggcaccctggtcgtggtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [301] c74>g|t76>a,S26>T(+ - +)|c86>g,T29>S(+ - +)|t105>g,D35>E(+ - +)|g106>a,D36>N(+ + -)|a132>g|g133>c,A45>P(- - -)|g150>a|g163>c,G55>H(+ - -)|g164>a,G55>H(+ - -)|t165>c,G55>H(+ - -)|t168>c|a184>g,S62>G(+ + -)|t186>c,S62>G(+ + -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|a190>g,S64>G(+ + -)|a195>c|g197>a,G66>D(- - -)|t201>c|t210>a|a232>g,I78>V(+ - +)|c248>g,A83>G(- + -)|c255>t|c265>t|g284>c,R95>T(- - -)|g286>a,A96>T(- - -)|g291>c,E97>D(+ - +)|a317>g,K106>R(+ + +)| c74>g|t76>a,S26>T(+ - +)| c86>g,T29>S(+ - +)|t105>g,D35>E(+ - +)|g106>a,D36>N(+ + -)| a132>g|g133>c,A45>P(- - -)|g150>a|g163>c,G55>H(+ - -)|g164>a,G55>H(+ - -)|t165>c,G55>H(+ - -)| t168>c|a184>g,S62>G(+ + -)|t186>c,S62>G(+ + -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|a190>g,S64>G(+ + -)|a195>c| g197>a,G66>D(- - -)|t201>c|t210>a|a232>g,I78>V(+ - +)|c248>g,A83>G(- + -)|c255>t|c265>t|g284>c,R95>T(- - -)|g286>a,A96>T(- - -)|g291>c,E97>D(+ - +)| a317>g,K106>R(+ + +)|a320>c,D107>A(- - -)|t321>c,D107>A(- - -)|a322>c| 319 (322) 225 (228) 196 29 (32) 9 (10) 20 (22) 5 6 2 4 (5) 1 (3) 0 0 3 4 3 0 1 78 5 3 2 1 1 0 0 0 0 0 0 0 1 0 1 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 0 1 0 1 51 51 45 6 2 4 1 2 0 1 0 0 0 0 2 0 0 0 30 24 17 7 2 5 2 1 0 2 1 0 0 1 0 0 0 0 117 114 104 10 4 6 1 2 2 1 0 0 0 1 2 1 0 0 7 (10) 7 6 1 (4) 0 (1) 1 (3) 1 0 0 0 (1) 0 (2) 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| gcact ggattcagctctgagaattatgcc atgcactgggtccggcagcctccagggaagggcctagagtgggtctcacac atcagttggaatggcgaaggcatc gactacgcggactcagtgaagggccgattcaccgtctccagagacaacggcaagaattccctgtatttgcaaatgaacagtctgacaactgacgacacggccttgtattactgt gcaagagccccttcagtcacattcagcgggcaggggcactactatgatctc ARAPSVTFSGQGHYYDL IGHV3-9 IGHD4-11 IGHJ2 ggattcagctctgagaattatgcc atgcactgggtccggcagcctccagggaagggcctagagtgggtctcacac atcagttggaatggcgaaggcatc gactacgcggactcagtgaagggccgattcaccgtctccagagacaacggcaagaattccctgtatttgcaaatgaacagtctgacaactgacgacacggccttgtattactgt gcaagagccccttcagtcacattcagcgggcaggggcactactatgatctc IGA1 26 213 16 10 8 14 8 12 6 2 10 8 JY8QFUQ01BQ3I3 16112 productive Homsap IGHV3-30*02 F, or Homsap IGHV3-30-5*02 F 881 90.23 194/215 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD6-19*01 F 1 8 8 14 8.8.14 [0.17.38.11] CAKEEFDTGWTQPGDW in-frame - ctggattcatcttcagtgactatggcatgcactgggtccgccaggctccaggcgaggggctggattgggtggcatttatacgatatgatggaaatgagatacactatccagactccgtgaggggccgattcaccatctccagagacaattccaagaacaccctatatctagaaatgaacaatgtgagacctgaggacacggctgtgtattactgtgcgaaggaggaatttgacactggctggacacaaccgggcgactggggccagggaacgctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [288] c86>t,T29>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a142>g,K48>E(+ - -)|g153>t,E51>D(+ - +)|g171>a|g188>a,S63>N(- - -)|a190>g,N64>E(+ - -)|t192>g,N64>E(+ - -)|a194>t,K65>I(- + -)|t196>c,Y66>H(+ - -)|g202>c,A68>P(- - -)|a215>g,K72>R(+ + +)|g258>c|g261>a|g267>a|c268>g,Q90>E(+ + -)|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|c280>g,L94>V(+ - +)|g286>c,A96>P(- - -)|a318>g| c86>t,T29>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| a142>g,K48>E(+ - -)|g153>t,E51>D(+ - +)| g171>a|g188>a,S63>N(- - -)|a190>g,N64>E(+ - -)|t192>g,N64>E(+ - -)|a194>t,K65>I(- + -)| t196>c,Y66>H(+ - -)|g202>c,A68>P(- - -)|a215>g,K72>R(+ + +)|g258>c|g261>a|g267>a|c268>g,Q90>E(+ + -)|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|c280>g,L94>V(+ - +)|g286>c,A96>P(- - -)| a318>g| 320 223 201 22 5 17 5 6 2 1 0 0 1 0 3 2 1 1 78 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 1 1 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 30 24 19 5 1 4 1 2 0 0 0 0 1 0 0 0 0 1 117 114 103 11 3 8 1 3 1 1 0 0 0 0 3 2 0 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ct ggattcatcttcagtgactatggc atgcactgggtccgccaggctccaggcgaggggctggattgggtggcattt atacgatatgatggaaatgagata cactatccagactccgtgaggggccgattcaccatctccagagacaattccaagaacaccctatatctagaaatgaacaatgtgagacctgaggacacggctgtgtattactgt gcgaaggaggaatttgacactggctggacacaaccgggcgac AKEEFDTGWTQPGD IGHV3-30 IGHD6-19 IGHJ4 ggattcatcttcagtgactatggc atgcactgggtccgccaggctccaggcgaggggctggattgggtggcattt atacgatatgatggaaatgagata cactatccagactccgtgaggggccgattcaccatctccagagacaattccaagaacaccctatatctagaaatgaacaatgtgagacctgaggacacggctgtgtattactgt gcgaaggaggaatttgacactggctggacacaaccgggcgac IGA1 21 213 13 8 8 14 5 7 3 1 10 7 JY8QFUQ01BQ9G8 8372 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 1017 96.77 210/217 nt NA Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD1-1*01 F 3 8 8 8 8.8.8 [1.17.38.11] CAVNWNLDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcacctttagcggctatgccatgagctgggtccgccaggctccagggaaggggctggactgggtctcatctattagttataatggtggtagcacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctccaaatgaacagcctgagagccgaggacacggccgtatattactgtgctgtgaactggaaccttgactcctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [273] a106>g,S36>G(+ + -)|g153>c,E51>D(+ - +)|g163>t,A55>S(- + -)|g172>t,G58>Y(+ - -)|g173>a,G58>Y(+ - -)|g176>a,S59>N(- - -)|g267>c| a106>g,S36>G(+ + -)| g153>c,E51>D(+ - +)|g163>t,A55>S(- + -)| g172>t,G58>Y(+ - -)|g173>a,G58>Y(+ - -)|g176>a,S59>N(- - -)| g267>c| g315>t|a316>g,K106>V(- - -)|a317>t,K106>V(- - -)|a318>g,K106>V(- - -)|g319>a| 314 (320) 219 (225) 212 (213) 7 (12) 1 (3) 6 (9) 1 (3) 2 (3) 0 0 0 0 0 (1) 0 2 0 2 (3) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 0 0 0 0 0 0 1 0 1 0 30 24 21 3 0 3 0 2 0 0 0 0 0 0 0 0 1 0 117 114 113 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 2 (8) 2 2 (3) 0 (5) 0 (2) 0 (3) 0 (2) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcggctatgcc atgagctgggtccgccaggctccagggaaggggctggactgggtctcatct attagttataatggtggtagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctccaaatgaacagcctgagagccgaggacacggccgtatattactgt gctgtgaactggaaccttgactcc AVNWNLDS IGHV3-23 IGHD1-1 IGHJ4 ggattcacctttagcggctatgcc atgagctgggtccgccaggctccagggaaggggctggactgggtctcatct attagttataatggtggtagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctccaaatgaacagcctgagagccgaggacacggccgtatattactgt gctgtgaactggaaccttgactcc IGG2 7 213 3 4 2 6 1 1 1 0 2 4 JY8QFUQ01BQAON 10520 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*04 F or Homsap IGHV3-33*06 F 815 86.24 188/218 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARPHHNSGYSFDHW in-frame + cgtctgatttcaacgtcggtgactttgacatgcactgggtccgccagactccagacaaggggctggagtgggtggcacttttttggtatgacggaaagaggaaatattatgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacaccctgtatctgcaaatggacagcctgagagccgacgacacggctacctacttttgtgcgaggcctcatcataatagtggttattcctttgaccactggggccagggaacccaggtcaccgtctcctctgcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcgtcgcatcgcctggtcaggcttcttcccccga NA IGA1 86 95 [288] g80>a,G27>D(- - -)|a81>t,G27>D(- - -)|c86>a,T29>N(- + -)|t88>g,F30>V(+ - -)|a103>g,S35>G(+ + -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>t,Y37>F(- + -)|g113>a,G38>D(- - -)|g133>a,A45>T(- - -)|g140>a,G47>D(- - -)|g163>c,V55>L(+ - +)|a166>t,I56>F(+ - -)|a168>t,I56>F(+ - -)|t177>c|g188>a,S63>K(- - -)|t189>g,S63>K(- - -)|a191>g,N64>R(+ - -)|t192>g,N64>R(+ - -)|c198>t|a225>g|g258>c|a274>g,N92>D(+ + -)|g291>c,E97>D(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>c,V101>T(- - -)|t306>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g80>a,G27>D(- - -)|a81>t,G27>D(- - -)|c86>a,T29>N(- + -)|t88>g,F30>V(+ - -)|a103>g,S35>G(+ + -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>t,Y37>F(- + -)|g113>a,G38>D(- - -)| g133>a,A45>T(- - -)|g140>a,G47>D(- - -)|g163>c,V55>L(+ - +)| a166>t,I56>F(+ - -)|a168>t,I56>F(+ - -)|t177>c|g188>a,S63>K(- - -)|t189>g,S63>K(- - -)|a191>g,N64>R(+ - -)|t192>g,N64>R(+ - -)| c198>t|a225>g|g258>c|a274>g,N92>D(+ + -)|g291>c,E97>D(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>c,V101>T(- - -)|t306>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| a318>g|g319>c|a320>c| 317 (320) 223 (226) 193 30 (33) 5 (8) 25 5 (6) 7 2 3 0 (1) 1 5 0 4 (5) 0 0 3 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 15 9 0 9 2 3 0 0 0 1 2 0 0 0 0 1 51 51 48 3 0 3 0 2 0 0 0 0 0 0 1 0 0 0 30 24 17 7 1 6 1 1 0 1 0 0 2 0 0 0 0 2 117 114 103 11 4 7 2 1 2 2 0 0 1 0 3 0 0 0 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cgtct gatttcaacgtcggtgactttgac atgcactgggtccgccagactccagacaaggggctggagtgggtggcactt ttttggtatgacggaaagaggaaa tattatgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacaccctgtatctgcaaatggacagcctgagagccgacgacacggctacctacttttgt gcgaggcctcatcataatagtggttattcctttgaccac ARPHHNSGYSFDH IGHV3-33 IGHD3-22 IGHJ4 gatttcaacgtcggtgactttgac atgcactgggtccgccagactccagacaaggggctggagtgggtggcactt ttttggtatgacggaaagaggaaa tattatgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacaccctgtatctgcaaatggacagcctgagagccgacgacacggctacctacttttgt gcgaggcctcatcataatagtggttattcctttgaccac IGA1 30 213 17 13 9 14 8 16 4 1 10 15 JY8QFUQ01BQCNP 20539 productive (see comment) Homsap IGHV4-61*01 F (see comment) 776 83.71 185/221 nt 83.26 184/221 nt Homsap IGHJ3*02 F (see comment) 178 84 42/50 nt Homsap IGHD3-22*01 F 2 10 7 13 10.7.13 [1.17.38.11] CARDGNRGYYSFETW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (83,71% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*01 and Homsap_IGHJ4*03 and Homsap_IGHJ5*01 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 45 of V-REGION: 'T' (1 nucleotides at position 54 to 54 in the user submitted sequence), (cause frameshift); tgtctggtggctccgtcagcagtggaaattcctactggacttggatccgccagTccccccgagaagggactggagtggcttgcatatattcgaaacactgggacaaccaactacaacccctccctcaagagtagactcaccatgtctctggacatgtctaggaatcagttctccctgaggctgaacgatgtgaccgctgcggacacggccatatattactgtgcgagagacggcaatagaggttattactcttttgaaacctggggccgagggacattggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtccaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [293] c75>g|t105>a|g107>a,S36>N(- - -)|a110>c,Y37>S(+ - -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g129>c|a138>c|g140>a,G47>E(- - -)|a157>c,I53>L(+ + +)|g161>c,G54>A(- + -)|g162>a,G54>A(- + -)|c168>t|t169>c,Y57>R(- - -)|a170>g,Y57>R(- - -)|t171>a,Y57>R(- - -)|t172>a,Y58>N(- - -)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)|c223>a|g226>c,V76>L(+ - +)|a234>g,I78>M(+ + -)|a237>t|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|c245>t,T82>M(- - -)|c249>t|a251>g,K84>R(+ + +)|c255>t|a269>g,K90>R(+ + +)|g275>a,S92>N(- - -)|t277>g,S93>D(- - -)|c278>a,S93>D(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| c75>g| t105>a|g107>a,S36>N(- - -)|a110>c,Y37>S(+ - -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g129>c|a138>c|g140>a,G47>E(- - -)|a157>c,I53>L(+ + +)|g161>c,G54>A(- + -)|g162>a,G54>A(- + -)| c168>t|t169>c,Y57>R(- - -)|a170>g,Y57>R(- - -)|t171>a,Y57>R(- - -)|t172>a,Y58>N(- - -)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)| c223>a|g226>c,V76>L(+ - +)|a234>g,I78>M(+ + -)|a237>t|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|c245>t,T82>M(- - -)|c249>t|a251>g,K84>R(+ + +)|c255>t|a269>g,K90>R(+ + +)|g275>a,S92>N(- - -)|t277>g,S93>D(- - -)|c278>a,S93>D(- - -)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 229 193 36 9 27 5 6 5 1 3 3 1 3 7 1 0 1 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 30 27 3 1 2 0 1 0 0 1 0 0 1 0 0 0 0 51 51 43 8 2 6 0 2 1 0 2 0 0 0 3 0 0 0 30 21 13 8 1 7 1 0 1 1 0 1 0 2 2 0 0 0 117 114 98 16 4 12 4 3 3 0 0 2 1 0 2 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tgtct ggtggctccgtcagcagtggaaattcctac tggacttggatccgccagccccccgagaagggactggagtggcttgcatat attcgaaacactgggacaacc aactacaacccctccctcaagagtagactcaccatgtctctggacatgtctaggaatcagttctccctgaggctgaacgatgtgaccgctgcggacacggccatatattactgt gcgagagacggcaatagaggttattactcttttgaaacc ARDGNRGYYSFET IGHV4-61 IGHD3-22 IGHJ3 ggtggctccgtcagcagtggaaattcctac tggacttggatccgccagccccccgagaagggactggagtggcttgcatat attcgaaacactgggacaacc aactacaacccctccctcaagagtagactcaccatgtctctggacatgtctaggaatcagttctccctgaggctgaacgatgtgaccgctgcggacacggccatatattactgt gcgagagacggcaatagaggttattactcttttgaaacc IGG1 35 216 17 18 11 21 6 14 6 2 18 9 JY8QFUQ01BQERB 7209 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 864 88.94 193/217 nt 88.48 192/217 nt Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD2-21*01 F 2 8 8 8 8.8.8 [1.17.38.11] CAKRYSLDSW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 40 of V-REGION: 'A' (1 nucleotides at position 33 to 33 in the user submitted sequence), (cause frameshift); ctctggattcacctttaagaactatgccttaaAactgggtccgccaggctccagggaaggggctgaagtgggtctccggaatcagtgctactggtgaaagcacacactacgcagactccgtaattggccggttcaccatctccagagacgattccaagaatacgttatatctgcaaatgaacagcctgagagccgaggacacggccgtatatttctgtgcgaaacgatattctcttgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccga NA IGA1 100 100 [273] g104>a,S35>K(- - -)|c105>g,S35>K(- - -)|g107>a,S36>N(- - -)|a115>t,M39>L(+ + -)|g117>a,M39>L(+ + -)|g119>a,S40>N(- - -)|g151>a,E51>K(+ - -)|a162>c|c164>g,A55>G(- + -)|t165>a,A55>G(- + -)|t168>c|g173>c,G58>A(- + -)|g176>c,S59>T(+ - +)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|t196>c,Y66>H(+ - -)|g213>a|a215>t,K72>I(- + -)|g216>t,K72>I(- + -)|a244>g,N82>D(+ + -)|c255>t|c259>t|g261>a|a308>t,Y103>F(- + -)| g104>a,S35>K(- - -)|c105>g,S35>K(- - -)|g107>a,S36>N(- - -)| a115>t,M39>L(+ + -)|g117>a,M39>L(+ + -)|g119>a,S40>N(- - -)|g151>a,E51>K(+ - -)|a162>c|c164>g,A55>G(- + -)|t165>a,A55>G(- + -)| t168>c|g173>c,G58>A(- + -)|g176>c,S59>T(+ - +)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)| t196>c,Y66>H(+ - -)|g213>a|a215>t,K72>I(- + -)|g216>t,K72>I(- + -)|a244>g,N82>D(+ + -)|c255>t|c259>t|g261>a|a308>t,Y103>F(- + -)| g319>c|a320>g| 318 (320) 223 (225) 199 24 (26) 6 (8) 18 1 (2) 8 2 2 1 0 3 2 2 (3) 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 2 0 0 0 0 0 0 0 1 0 0 51 51 44 7 1 6 0 3 0 0 1 0 1 1 0 1 0 0 30 24 19 5 1 4 0 1 0 1 0 0 0 1 2 0 0 0 117 114 105 9 4 5 1 2 2 1 0 0 2 0 0 0 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaagaactatgcc ttaaactgggtccgccaggctccagggaaggggctgaagtgggtctccgga atcagtgctactggtgaaagcaca cactacgcagactccgtaattggccggttcaccatctccagagacgattccaagaatacgttatatctgcaaatgaacagcctgagagccgaggacacggccgtatatttctgt gcgaaacgatattctcttgactcc AKRYSLDS IGHV3-23 IGHD2-21 IGHJ4 ggattcacctttaagaactatgcc ttaaactgggtccgccaggctccagggaaggggctgaagtgggtctccgga atcagtgctactggtgaaagcaca cactacgcagactccgtaattggccggttcaccatctccagagacgattccaagaatacgttatatctgcaaatgaacagcctgagagccgaggacacggccgtatatttctgt gcgaaacgatattctcttgactcc IGA1 24 213 13 11 10 15 3 9 5 1 11 7 JY8QFUQ01BQLNQ 15565 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 941 92.66 202/218 nt 92.2 201/218 nt Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD3-16*01 F 2 8 8 16 8.8.16 [1.17.38.11] CARRLNNYDSGEYAMDVW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'G' (1 nucleotides at position 9 to 9 in the user submitted sequence), (cause frameshift); gttctggaGtacacgtttatgaattactggatcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatcatctatcctggtacctctgagaccagatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgccttcgtgcagtggagcagcctgaaggccccggacaccgccatatattactgtgcgaggcgactcaataattacgactcgggagagtacgctatggacgtctggggccaagggaccacggtcaccgtctcctcaacctccaccaagggcccatcggtcttccccctggcaccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 86 100 [299] g86>c,S29>T(+ - +)|c87>g,S29>T(+ - +)|c104>t,T35>M(- - -)|c105>g,T35>M(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g184>a,D62>T(- + -)|a185>c,D62>T(- + -)|t192>g,D64>E(+ - +)|c213>t|c234>t|t251>c,I84>T(- - -)|a263>t,Y88>F(- + -)|c265>g,L89>V(+ - +)|t289>c,S97>P(+ - -)|g303>a,M101>I(+ + -)| g86>c,S29>T(+ - +)|c87>g,S29>T(+ - +)|c104>t,T35>M(- - -)|c105>g,T35>M(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g184>a,D62>T(- + -)|a185>c,D62>T(- + -)|t192>g,D64>E(+ - +)| c213>t|c234>t|t251>c,I84>T(- - -)|a263>t,Y88>F(- + -)|c265>g,L89>V(+ - +)|t289>c,S97>P(+ - -)|g303>a,M101>I(+ + -)| a318>g|a320>g| 317 (320) 223 (226) 207 (208) 16 (18) 2 (4) 14 0 (2) 3 4 2 1 0 1 0 1 3 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 1 2 0 0 0 0 0 1 2 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 1 0 0 1 0 0 0 0 0 0 1 117 114 107 7 2 5 0 1 2 2 0 0 1 0 0 1 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| gttct ggatacacgtttatgaattactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtacctctgagacc agatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgccttcgtgcagtggagcagcctgaaggccccggacaccgccatatattactgt gcgaggcgactcaataattacgactcgggagagtacgctatggacgtc ARRLNNYDSGEYAMDV IGHV5-51 IGHD3-16 IGHJ6 ggatacacgtttatgaattactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtacctctgagacc agatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgccttcgtgcagtggagcagcctgaaggccccggacaccgccatatattactgt gcgaggcgactcaataattacgactcgggagagtacgctatggacgtc IGG2 16 213 9 7 7 11 2 5 2 0 5 9 JY8QFUQ01BQMY8 15811 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 1031 97.25 212/218 nt NA Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD2-15*01 F 2 8 8 17 8.8.17 [1.17.38.11] CARPGGIAYCSGGSCYDPW in-frame + cctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacatattaatattgatgggagtaccacagactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgtgcaagaccaggggggattgcctattgtagtggtggtagctgctacgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [300] g164>a,R55>H(- - +)|g173>t,S58>I(- - -)|g191>c,S64>T(+ - +)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|g258>a| g164>a,R55>H(- - +)| g173>t,S58>I(- - -)|g191>c,S64>T(+ - +)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|g258>a| g319>c|a320>c| 318 (320) 224 (226) 218 6 (8) 1 (3) 5 1 3 0 0 0 (1) 0 0 0 1 (2) 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 0 0 1 0 1 0 117 114 111 3 1 2 1 2 0 0 0 0 0 0 0 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacat attaatattgatgggagtaccaca gactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt gcaagaccaggggggattgcctattgtagtggtggtagctgctacgacccc ARPGGIAYCSGGSCYDP IGHV3-74 IGHD2-15 IGHJ5 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacat attaatattgatgggagtaccaca gactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt gcaagaccaggggggattgcctattgtagtggtggtagctgctacgacccc IGA2 6 213 4 2 3 5 1 1 1 0 3 2 JY8QFUQ01BQQG5 16686 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*04 F or Homsap IGHV3-30*15 F or Homsap IGHV3-30*18 F or Homsap IGHV3-30-3*03 F or Homsap IGHV3-30-5*01 F (see comment) 693 80.18 174/217 nt NA Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD1-1*01 F 3 8 8 11 8.8.11 [1.17.38.11] CVRSPNHNWFDPW in-frame - Low V-REGION identity (80,18% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctggattcaaattcaataattatgcaatgcactgggtccgccagtctccaagagcggggctgcaatgggtggcggctatatcgactgacggcaataaagaatatcatgcagactccgtgaagggccggctcaccctctccagagacaattcgaggagtacgctgtctctgcaattgcgcaacctgacagctgacgactcggctctgtattattgtgtcagatctcccaaccacaattggttcgacccctggggccagggagccctggtcactgtcacctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [281] c86>a,T29>K(- - -)|c87>a,T29>K(- - -)|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g113>c,G38>A(- + -)|c114>a,G38>A(- + -)|g133>t,A45>S(- + -)|g139>a,G47>R(- - -)|c141>a,G47>R(- - -)|a142>g,K48>A(- - -)|a143>c,K48>A(- - -)|g151>c,E51>Q(+ + -)|g153>a,E51>Q(+ + -)|a162>g|t164>c,V55>A(+ - +)|a171>g|t172>a,Y58>T(+ - -)|a173>c,Y58>T(+ - -)|t177>c|a186>c|g188>a,S63>N(- - -)|t192>a,N64>K(+ - -)|a193>g,K65>E(+ - -)|c198>t|t199>c,Y67>H(+ - -)|a225>g|t226>c,F76>L(+ - -)|a232>c,I78>L(+ + +)|c249>g|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a263>c,Y88>S(+ - -)|a271>t,M91>L(+ + -)|a274>c,N92>R(+ - -)|a275>g,N92>R(+ - -)|g278>a,S93>N(- - -)|g284>c,R95>T(- - -)|g291>c,E97>D(+ - +)|a295>t,T99>S(+ - +)|g301>c,V101>L(+ - +)|c309>t|c314>t,A105>V(+ - +)|g315>c,A105>V(+ - +)| c86>a,T29>K(- - -)|c87>a,T29>K(- - -)|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g113>c,G38>A(- + -)|c114>a,G38>A(- + -)| g133>t,A45>S(- + -)|g139>a,G47>R(- - -)|c141>a,G47>R(- - -)|a142>g,K48>A(- - -)|a143>c,K48>A(- - -)|g151>c,E51>Q(+ + -)|g153>a,E51>Q(+ + -)|a162>g|t164>c,V55>A(+ - +)| a171>g|t172>a,Y58>T(+ - -)|a173>c,Y58>T(+ - -)|t177>c|a186>c|g188>a,S63>N(- - -)|t192>a,N64>K(+ - -)|a193>g,K65>E(+ - -)| c198>t|t199>c,Y67>H(+ - -)|a225>g|t226>c,F76>L(+ - -)|a232>c,I78>L(+ + +)|c249>g|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a263>c,Y88>S(+ - -)|a271>t,M91>L(+ + -)|a274>c,N92>R(+ - -)|a275>g,N92>R(+ - -)|g278>a,S93>N(- - -)|g284>c,R95>T(- - -)|g291>c,E97>D(+ - +)|a295>t,T99>S(+ - +)|g301>c,V101>L(+ - +)|c309>t| c314>t,A105>V(+ - +)|g315>c,A105>V(+ - +)|g319>t|a320>c| 318 (320) 223 (225) 178 45 (47) 8 (10) 37 8 6 5 4 6 (7) 4 2 2 6 1 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 0 7 0 2 1 0 0 3 0 0 1 0 0 0 51 51 42 9 1 8 2 2 0 1 1 1 0 0 1 0 1 0 30 24 16 8 3 5 2 1 0 1 2 0 0 2 0 0 0 0 117 114 95 19 4 15 4 1 3 2 3 0 2 0 3 1 0 0 6 (8) 6 4 2 (4) 0 (2) 2 0 0 1 0 0 (1) 0 0 0 1 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaaattcaataattatgca atgcactgggtccgccagtctccaagagcggggctgcaatgggtggcggct atatcgactgacggcaataaagaa tatcatgcagactccgtgaagggccggctcaccctctccagagacaattcgaggagtacgctgtctctgcaattgcgcaacctgacagctgacgactcggctctgtattattgt gtcagatctcccaaccacaattggttcgacccc VRSPNHNWFDP IGHV3-30 IGHD1-1 IGHJ5 ggattcaaattcaataattatgca atgcactgggtccgccagtctccaagagcggggctgcaatgggtggcggct atatcgactgacggcaataaagaa tatcatgcagactccgtgaagggccggctcaccctctccagagacaattcgaggagtacgctgtctctgcaattgcgcaacctgacagctgacgactcggctctgtattattgt gtcagatctcccaaccacaattggttcgacccc IGA1 43 213 22 21 10 21 12 22 5 3 23 12 JY8QFUQ01BR1YM 7343 productive Homsap IGHV3-7*01 F 1008 96.31 209/217 nt NA Homsap IGHJ4*02 F 78 62.5 30/48 nt Homsap IGHD1-26*01 F 1 8 8 13 8.8.13 [1.17.38.11] CATSPSVGSTTVGYW in-frame + ctctggattcacctttagaaactattggatgagttgggtccgccaggctccagggaaggggctggagtgggtggccaacataatgacagatggaggtgacaaatactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgccacatcccctagcgtgggatctacgacggtgggctactggggccggggaaccctggtaaccggtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 100 100 [288] t105>a,S35>R(- - -)|g107>a,S36>N(- - -)|c120>t|a170>t,K57>M(- + -)|c172>a,Q58>T(- - -)|a173>c,Q58>T(- - -)|a187>g,S63>G(+ + -)|g192>c,E64>D(+ - +)| t105>a,S35>R(- - -)|g107>a,S36>N(- - -)| c120>t| a170>t,K57>M(- + -)|c172>a,Q58>T(- - -)|a173>c,Q58>T(- - -)|a187>g,S63>G(+ + -)|g192>c,E64>D(+ - +)| g315>c|g317>c,R106>T(- - -)|g319>t|a320>c| 314 (320) 219 (225) 211 (213) 8 (12) 1 (4) 7 (8) 1 1 1 0 1 (2) 1 1 1 1 (3) 0 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 1 0 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 19 5 0 5 1 0 0 0 1 1 1 0 1 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 (8) 2 2 (4) 0 (4) 0 (3) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (2) 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagaaactattgg atgagttgggtccgccaggctccagggaaggggctggagtgggtggccaac ataatgacagatggaggtgacaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gccacatcccctagcgtgggatctacgacggtgggctac ATSPSVGSTTVGY IGHV3-7 IGHD1-26 IGHJ4 ggattcacctttagaaactattgg atgagttgggtccgccaggctccagggaaggggctggagtgggtggccaac ataatgacagatggaggtgacaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gccacatcccctagcgtgggatctacgacggtgggctac IGA2 8 213 3 5 2 4 1 4 1 0 0 7 JY8QFUQ01BR9Q4 9962 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 837 87.56 190/217 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD3-3*01 F 2 8 8 13 8.8.13 [1.17.38.11] CAKRWRASNGHFDYW in-frame + ctctggattcacctttagcagcttttccatggggtgggtccgccaggctccagggcggggactggagtgggtctcagccattgacggtcttactggtggtacatattacgcggactccgtgaggggccgattcaccatctccagagacgattccaagaacacactgtatctgcaaatgaatagcctgagagccgaggacacagccatatatcactgtgcgaagcggtggcgtgcttccaatggccactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaaaggatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 79 89 [287, 289] a110>t,Y37>F(- + -)|g112>t,A38>S(- + -)|a118>g,S40>G(+ + -)|c120>g,S40>G(+ + -)|a142>c,K48>R(+ + +)|a143>g,K48>R(+ + +)|g147>a|t165>c|a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|t171>c,S57>D(- - -)|a175>c,S59>L(- - -)|g176>t,S59>L(- - -)|g184>a,G62>T(+ - -)|g185>c,G62>T(+ - -)|a190>g,S64>G(+ + -)|c192>t,S64>G(+ + -)|c198>t|a204>g|a215>g,K72>R(+ + +)|g225>a|a244>g,N82>D(+ + -)|g258>a|c276>t|g297>a|g301>a,V101>I(+ - +)|t307>c,Y103>H(+ - -)| a110>t,Y37>F(- + -)|g112>t,A38>S(- + -)| a118>g,S40>G(+ + -)|c120>g,S40>G(+ + -)|a142>c,K48>R(+ + +)|a143>g,K48>R(+ + +)|g147>a|t165>c| a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|t171>c,S57>D(- - -)|a175>c,S59>L(- - -)|g176>t,S59>L(- - -)|g184>a,G62>T(+ - -)|g185>c,G62>T(+ - -)|a190>g,S64>G(+ + -)|c192>t,S64>G(+ + -)| c198>t|a204>g|a215>g,K72>R(+ + +)|g225>a|a244>g,N82>D(+ + -)|g258>a|c276>t|g297>a|g301>a,V101>I(+ - +)|t307>c,Y103>H(+ - -)| a318>g|g319>c|a320>g| 317 (320) 222 (225) 195 27 (30) 8 (11) 19 7 (9) 7 3 3 2 0 1 0 1 (2) 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 0 0 1 0 51 51 45 6 2 4 2 1 0 1 1 0 0 0 0 1 0 0 30 24 15 9 0 9 2 2 1 1 1 0 0 0 1 0 1 0 117 114 104 10 6 4 3 4 2 1 0 0 0 0 0 0 0 0 5 (8) 5 5 0 (3) 0 (3) 0 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcagcttttcc atggggtgggtccgccaggctccagggcggggactggagtgggtctcagcc attgacggtcttactggtggtaca tattacgcggactccgtgaggggccgattcaccatctccagagacgattccaagaacacactgtatctgcaaatgaatagcctgagagccgaggacacagccatatatcactgt gcgaagcggtggcgtgcttccaatggccactttgactac AKRWRASNGHFDY IGHV3-23 IGHD3-3 IGHJ4 ggattcacctttagcagcttttcc atggggtgggtccgccaggctccagggcggggactggagtgggtctcagcc attgacggtcttactggtggtaca tattacgcggactccgtgaggggccgattcaccatctccagagacgattccaagaacacactgtatctgcaaatgaatagcctgagagccgaggacacagccatatatcactgt gcgaagcggtggcgtgcttccaatggccactttgactac IGA2 27 213 20 7 10 14 10 13 8 0 8 11 JY8QFUQ01BRBL7 18383 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F (see comment) 773 84.65 182/215 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-21*02 F 3 8 7 15 8.7.15 [1.17.38.11] CARGRTVVTVIDYIDYW in-frame + Low V-REGION identity (84,65% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctgggggactccattagtggttactattggacgtggatccggcagaccccagggaagggactggagtggattggaaatgtccattacactgggagtaccaagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgtgcgagaggacgaacggtggtgactgttattgactacattgactattggggtcagggagccctggtcagcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [291] t74>c|c75>t|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)|t81>g|g83>a,G28>D(- - -)|c90>t|a106>g,S36>G(+ + -)|c114>t|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>a,P45>T(+ + -)|g162>a|t163>a,Y55>N(- - -)|a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|c192>t|c198>g,N66>K(+ - -)|a203>g,N68>S(- - -)|a234>g,I78>M(+ + -)|a240>t|c245>t,T82>M(- - -)|a251>g,K84>R(+ + +)|t262>a,S88>T(+ - +)|g267>c|g270>a|c271>t|g275>c,S92>T(+ - +)|g282>a|a283>g,T95>A(- - -)|g303>c| t74>c|c75>t|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)| t81>g|g83>a,G28>D(- - -)|c90>t|a106>g,S36>G(+ + -)|c114>t| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>a,P45>T(+ + -)|g162>a|t163>a,Y55>N(- - -)| a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|c192>t| c198>g,N66>K(+ - -)|a203>g,N68>S(- - -)|a234>g,I78>M(+ + -)|a240>t|c245>t,T82>M(- - -)|a251>g,K84>R(+ + +)|t262>a,S88>T(+ - +)|g267>c|g270>a|c271>t|g275>c,S92>T(+ - +)|g282>a|a283>g,T95>A(- - -)|g303>c| a320>g| 319 (320) 222 (223) 189 33 (34) 13 (14) 20 6 (7) 4 7 3 0 1 1 2 5 2 0 2 78 5 0 5 2 3 0 0 2 2 0 0 0 0 0 0 0 1 36 24 19 5 3 2 1 1 2 0 0 0 0 0 0 0 0 1 51 51 46 5 1 4 0 1 0 0 0 1 0 1 1 1 0 0 30 21 17 4 1 3 1 0 1 1 0 0 0 0 1 0 0 0 117 114 100 14 6 8 4 2 2 0 0 0 1 1 3 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctctg ggggactccattagtggttactat tggacgtggatccggcagaccccagggaagggactggagtggattggaaat gtccattacactgggagtacc aagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttattgactacattgactat ARGRTVVTVIDYIDY IGHV4-59 IGHD2-21 IGHJ4 ggggactccattagtggttactat tggacgtggatccggcagaccccagggaagggactggagtggattggaaat gtccattacactgggagtacc aagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttattgactacattgactat IGA2 28 210 16 12 9 17 7 11 7 4 12 5 JY8QFUQ01BREFM 16487 productive Homsap IGHV3-9*01 F 864 88.94 193/217 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD2-21*02 F 3 8 8 9 8.8.9 [1.17.38.11] CARDQVTEDYW in-frame - ctctggattcacttttgatgactctgccatgcactgggtgcggcaagctccagggaagggcccggagtgggtcgcaggtattagtggaaatagtggaaatataggatatgcggactcagtgaagggccgatgcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgtgccagagatcaagtgactgaagactactggggccagggaaccctggtcagtgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [276] c87>t|t108>c|a110>c,Y37>S(+ - -)|c126>g|t149>c,L50>P(- - -)|t160>g,S54>A(- + -)|t172>g,W58>G(- - -)|g174>a,W58>G(- - -)|t189>a|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)|c198>a|t210>a|t227>g,F76>C(+ - -)|c248>g,A83>G(- + -)|c255>t|c258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t288>c|g297>c|c300>t|t301>a,L101>M(+ + -)|c309>t|a315>c|a317>g,K106>R(+ + +)| c87>t|t108>c|a110>c,Y37>S(+ - -)| c126>g|t149>c,L50>P(- - -)|t160>g,S54>A(- + -)| t172>g,W58>G(- - -)|g174>a,W58>G(- - -)|t189>a|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)| c198>a|t210>a|t227>g,F76>C(+ - -)|c248>g,A83>G(- + -)|c255>t|c258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t288>c|g297>c|c300>t|t301>a,L101>M(+ + -)|c309>t| a315>c|a317>g,K106>R(+ + +)|a322>c| 321 (322) 226 (227) 200 26 (27) 13 (14) 13 1 3 5 3 2 (3) 2 0 3 1 3 0 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 1 1 1 0 0 0 0 0 0 0 51 51 48 3 1 2 0 0 0 1 0 0 0 0 0 1 0 1 30 24 19 5 1 4 0 2 1 0 0 0 0 1 0 0 0 1 117 114 101 13 8 5 0 1 3 1 0 2 0 2 1 2 0 1 9 (10) 9 7 2 (3) 1 (2) 1 1 0 0 0 1 (2) 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacttttgatgactctgcc atgcactgggtgcggcaagctccagggaagggcccggagtgggtcgcaggt attagtggaaatagtggaaatata ggatatgcggactcagtgaagggccgatgcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac ARDQVTEDY IGHV3-9 IGHD2-21 IGHJ4 ggattcacttttgatgactctgcc atgcactgggtgcggcaagctccagggaagggcccggagtgggtcgcaggt attagtggaaatagtggaaatata ggatatgcggactcagtgaagggccgatgcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac IGG1 24 213 11 13 8 14 3 10 9 3 7 5 JY8QFUQ01BRF2O 16245 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 950 93.12 203/218 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD3-10*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKKYHYGSGSYLYSFDYW in-frame + cctctgggttcacctttgcccactttgccatgacctgggtccgccaggctccagggaaggggctggagtgggtctcaactattagtggtggtgatgattccacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatggacagcctgagagccgaggacacggccgtatatcactgtgcgaagaagtatcactatggttcggggagttatctctactcctttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [300] a81>g|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a110>t,Y37>F(- + -)|g119>c,S40>T(+ - +)|g163>a,A55>T(- - -)|a175>g,S59>G(+ + -)|g185>a,G62>D(- - -)|g188>a,G63>D(- - -)|a190>t|g191>c|a274>g,N92>D(+ + -)|t307>c,Y103>H(+ - -)| a81>g|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a110>t,Y37>F(- + -)| g119>c,S40>T(+ - +)|g163>a,A55>T(- - -)| a175>g,S59>G(+ + -)|g185>a,G62>D(- - -)|g188>a,G63>D(- - -)|a190>t|g191>c| a274>g,N92>D(+ + -)|t307>c,Y103>H(+ - -)| a318>g|g319>a| 317 (320) 223 (226) 208 (209) 15 (17) 3 (5) 12 4 (5) 4 (5) 0 1 1 0 2 0 3 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 1 5 2 1 0 0 1 0 1 0 1 0 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 30 24 19 5 2 3 1 2 0 0 0 0 1 0 1 0 0 0 117 114 112 2 0 2 1 0 0 1 0 0 0 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct gggttcacctttgcccactttgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtggtggtgatgattccaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatggacagcctgagagccgaggacacggccgtatatcactgt gcgaagaagtatcactatggttcggggagttatctctactcctttgactac AKKYHYGSGSYLYSFDY IGHV3-23 IGHD3-10 IGHJ4 gggttcacctttgcccactttgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtggtggtgatgattccaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatggacagcctgagagccgaggacacggccgtatatcactgt gcgaagaagtatcactatggttcggggagttatctctactcctttgactac IGA1 15 213 9 6 4 7 5 8 0 3 4 8 JY8QFUQ01BRIHD 17295 productive Homsap IGHV3-7*01 F 1009 95.89 210/219 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 11 8.8.11 [2.17.38.11] CAISSGYYSTGYW in-frame - ggctctggattcagctttagttactattggatgagctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaaggaagatggaagtgagagacactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgtgcgattagtagtggttattactcgacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [283] c74>g,A25>G(- + -)|c86>g,T29>S(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)|t196>c,Y66>H(+ - -)|a254>g,N85>S(- - -)|g303>a| c74>g,A25>G(- + -)| c86>g,T29>S(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)| c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)| t196>c,Y66>H(+ - -)|a254>g,N85>S(- - -)|g303>a| g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 316 (320) 223 (227) 214 9 (13) 1 (3) 8 (10) 2 (3) 2 (3) 0 1 0 0 1 (2) 0 0 3 0 (1) 0 78 6 5 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 36 24 21 3 0 3 0 1 0 0 0 0 1 0 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 1 0 0 117 114 111 3 1 2 1 1 0 1 0 0 0 0 0 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ggctct ggattcagctttagttactattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgattagtagtggttattactcgacgggctac AISSGYYSTGY IGHV3-7 IGHD3-22 IGHJ4 ggattcagctttagttactattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgattagtagtggttattactcgacgggctac IGA1 8 213 5 3 2 4 3 4 1 0 2 5 JY8QFUQ01BRQMG 17282 productive Homsap IGHV6-1*01 F, or Homsap IGHV6-1*02 F 970 91.34 211/231 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD1-14*01 ORF 3 10 9 19 10.9.19 [3.17.38.11] CARASRRDRQSDRNYYELDFW in-frame - catctacccggggacagtgtctctagcaacagtgccacttggaactggatcaggcagtccccaacgggaggccttgagtggctgggaaggacatcctacaggtccaaatggtatagtgattatgcggtgtctgtgaaaagtcgaataaccatcaacccagacacatccaagaaccagttctccctgcaattgaactccgttagtcccgaggacacggctgtgtattactgtgcaagagcttccagacgcgatcgtcagtctgatcggaactactatgagctggacttctggggccagggaatcttggtcgccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [319] g70>c,A24>H(- - -)|c71>a,A24>H(- - -)|c72>t,A24>H(- - -)|a73>c,I25>L(+ + +)|c75>a,I25>L(+ + +)|t76>c,S26>P(+ - -)|t111>c|g112>a,A38>T(- - -)|t139>a,S47>T(+ - +)|a142>g,R48>G(- - -)|a170>c,Y57>S(+ - -)|g186>a|a194>g,N65>S(- - -)|a204>g|a207>g|g270>a|c271>t|t279>c|g282>t|c284>g,T95>S(+ - +)| g70>c,A24>H(- - -)|c71>a,A24>H(- - -)|c72>t,A24>H(- - -)|a73>c,I25>L(+ + +)|c75>a,I25>L(+ + +)|t76>c,S26>P(+ - -)| t111>c|g112>a,A38>T(- - -)| t139>a,S47>T(+ - +)|a142>g,R48>G(- - -)| a170>c,Y57>S(+ - -)|g186>a|a194>g,N65>S(- - -)| a204>g|a207>g|g270>a|c271>t|t279>c|g282>t|c284>g,T95>S(+ - +)| a320>c| 319 (320) 238 (239) 218 20 (21) 8 (9) 12 4 3 2 3 2 (3) 2 0 1 1 1 1 0 78 9 3 6 0 6 0 0 1 1 1 2 0 0 1 0 0 0 36 30 28 2 1 1 0 1 0 1 0 0 0 0 0 0 0 0 51 51 49 2 0 2 1 0 0 0 0 0 0 1 0 0 0 0 30 27 24 3 1 2 1 1 0 0 1 0 0 0 0 0 0 0 117 114 107 7 6 1 2 1 1 1 0 0 0 0 0 1 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,5-6(FR1)|aa,40-41(FR1)|aa,103-104(CDR1)|aa,118-119(FR2)|aa,162-163(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,184-185(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|ta,206-207(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,227-228(FR3)|aa,235-236(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,5-6(FR1)|tt,114-115(CDR1)|tt,149-150(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|tt,198-199(FR3)|ta,206-207(FR3)|ta,227-228(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggta,3-6(FR1)|agct,8-11(FR1)|agca,14-17(FR1)|agca,100-103(CDR1)|ggca,128-131(FR2)|ggct,155-158(FR2)|ggta,188-191(CDR2)|agta,204-207(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tgcc,69-72(FR1)|tgct,108-111(CDR1)|aact,118-121(FR2)|tact,169-172(CDR2)|aacc,228-231(FR3)|aacc,235-238(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|aact,274-277(FR3)|tact,307-310(FR3)| catctaccc ggggacagtgtctctagcaacagtgccact tggaactggatcaggcagtccccaacgggaggccttgagtggctgggaagg acatcctacaggtccaaatggtatagt gattatgcggtgtctgtgaaaagtcgaataaccatcaacccagacacatccaagaaccagttctccctgcaattgaactccgttagtcccgaggacacggctgtgtattactgt gcaagagcttccagacgcgatcgtcagtctgatcggaactactatgagctggacttc ARASRRDRQSDRNYYELDF IGHV6-1 IGHD1-14 IGHJ4 ggggacagtgtctctagcaacagtgccact tggaactggatcaggcagtccccaacgggaggccttgagtggctgggaagg acatcctacaggtccaaatggtatagt gattatgcggtgtctgtgaaaagtcgaataaccatcaacccagacacatccaagaaccagttctccctgcaattgaactccgttagtcccgaggacacggctgtgtattactgt gcaagagcttccagacgcgatcgtcagtctgatcggaactactatgagctggacttc IGA1 14 222 10 4 4 6 6 8 6 2 3 3 JY8QFUQ01BRZIH 15189 productive (see comment) Homsap IGHV3-48*02 F (see comment) 883 89.5 196/219 nt 89.04 195/219 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-10*01 F 1 8 8 14 8.8.14 [2.17.38.11] FVTERGGGLLRYLDLW in-frame - Potentially, because of detected insertion/deletion Cys 104 is missing in the submitted sequence this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 62 to 62 in the user submitted sequence), (cause frameshift); ggctctggattcatgttcggtagttatagtctgaattgggtccgccaggctccagggaaggGggctggagtggatttcatatattagtagcagtagtcaaacgatttactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgcgagacgacgacacggccatttactactttgtgacagagaggggcgggggcctattacgatatttggacttatggggcctgggaactcaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [293] c74>g,A25>G(- + -)|c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|a103>g,S35>G(+ + -)|c108>t|c114>t|a115>c,M39>L(+ + -)|c120>t|g157>a,V53>I(+ - +)|c165>t|t174>c|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>a,S63>Q(- - -)|c192>g|a195>t|a283>c|g291>c,E97>D(+ - +)|t300>c|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|t306>c|g311>t,C104>F(+ - -)|c314>t,A105>V(+ - +)| c74>g,A25>G(- + -)| c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|a103>g,S35>G(+ + -)|c108>t|c114>t| a115>c,M39>L(+ + -)|c120>t|g157>a,V53>I(+ - +)|c165>t| t174>c|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>a,S63>Q(- - -)|c192>g|a195>t| a283>c|g291>c,E97>D(+ - +)|t300>c|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|t306>c|g311>t,C104>F(+ - -)| c314>t,A105>V(+ - +)|g317>c,R106>T(- - -)| 320 227 202 25 10 15 1 3 6 3 3 0 1 1 2 3 2 0 78 6 5 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 36 24 19 5 2 3 1 0 3 0 0 0 0 0 0 1 0 0 51 51 47 4 2 2 0 1 2 0 1 0 0 0 0 0 0 0 30 24 18 6 3 3 0 1 0 1 1 0 1 1 0 1 0 0 117 114 107 7 3 4 0 1 0 2 1 0 0 0 1 0 2 0 8 8 6 2 0 2 0 0 1 0 0 0 0 0 1 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ggctct ggattcatgttcggtagttatagt ctgaattgggtccgccaggctccagggaaggggctggagtggatttcatat attagtagcagtagtcaaacgatt tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgcgagacgacgacacggccatttactacttt gtgacagagaggggcgggggcctattacgatatttggactta VTERGGGLLRYLDL IGHV3-48 IGHD3-10 IGHJ4 ggattcatgttcggtagttatagt ctgaattgggtccgccaggctccagggaaggggctggagtggatttcatat attagtagcagtagtcaaacgatt tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgcgagacgacgacacggccatttactacttt gtgacagagaggggcgggggcctattacgatatttggactta IGA2 22 213 12 10 8 13 4 9 5 5 6 6 JY8QFUQ01BS2Q3 10367 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 837 87.56 190/217 nt NA Homsap IGHJ6*02 F 112 64.52 40/62 nt Homsap IGHD6-19*01 F 1 8 8 12 8.8.12 [1.17.38.11] CAGSGRDPYGINVW in-frame + ctctggattcaccttcaattcctataccatgatgtgggtccgccaggctccggggaagggactggagtgggtctcaaccattagtcctagtagtcagtacatatactatgcagactctgtggagggccgattcaccatctccagagtcgacgcccggagttcagtgtttctgcaaatgaacagcctgagagacgacgacacggctgtgtattactgtgcgggcagtggcagggatccgtacggtattaacgtctggggccaagggaccacggtcacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [283] g104>a,S35>N(- - -)|a106>t|g107>c|g113>c,S38>T(+ - +)|a119>t,N40>M(- - -)|c120>g,N40>M(- - -)|a138>g|g147>a|t163>a,S55>T(+ - +)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>g,S63>Q(- - -)|c201>t|a210>t|a214>g,K72>E(+ - -)|a242>t,D81>V(- - -)|a244>g,N82>D(+ + -)|a250>c,K84>R(+ + +)|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c287>a,A96>D(- - -)|g291>c,E97>D(+ - +)| g104>a,S35>N(- - -)|a106>t|g107>c|g113>c,S38>T(+ - +)| a119>t,N40>M(- - -)|c120>g,N40>M(- - -)|a138>g|g147>a|t163>a,S55>T(+ - +)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>Q(- - -)|g188>a,S63>Q(- - -)|t189>g,S63>Q(- - -)| c201>t|a210>t|a214>g,K72>E(+ - -)|a242>t,D81>V(- - -)|a244>g,N82>D(+ + -)|a250>c,K84>R(+ + +)|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c287>a,A96>D(- - -)|g291>c,E97>D(+ - +)| a316>g,R106>G(- - -)|a318>c,R106>G(- - -)|g319>a|a320>g| 315 (320) 220 (225) 193 (194) 27 (31) 6 (8) 21 (23) 5 (7) 3 (4) 2 0 3 (4) 1 5 1 4 2 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 1 0 0 0 0 1 0 2 0 0 0 51 51 46 5 2 3 1 1 0 0 0 0 1 1 0 1 0 0 30 24 19 5 0 5 0 1 0 0 2 0 0 0 1 0 0 1 117 114 101 13 2 11 4 0 2 0 1 1 3 0 1 1 0 0 3 (8) 3 3 (4) 0 (4) 0 (2) 0 (2) 0 (2) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcaattcctatacc atgatgtgggtccgccaggctccggggaagggactggagtgggtctcaacc attagtcctagtagtcagtacata tactatgcagactctgtggagggccgattcaccatctccagagtcgacgcccggagttcagtgtttctgcaaatgaacagcctgagagacgacgacacggctgtgtattactgt gcgggcagtggcagggatccgtacggtattaacgtc AGSGRDPYGINV IGHV3-21 IGHD6-19 IGHJ6 ggattcaccttcaattcctatacc atgatgtgggtccgccaggctccggggaagggactggagtgggtctcaacc attagtcctagtagtcagtacata tactatgcagactctgtggagggccgattcaccatctccagagtcgacgcccggagttcagtgtttctgcaaatgaacagcctgagagacgacgacacggctgtgtattactgt gcgggcagtggcagggatccgtacggtattaacgtc IGA2 27 213 10 17 5 12 5 15 4 2 14 7 JY8QFUQ01BS2WD 5106 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 932 92.2 201/218 nt 91.74 200/218 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 3 8 8 12 8.8.12 [1.17.38.11] CARDTGQWKDFDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 61 to 61 in the user submitted sequence), (cause frameshift); cctctggattcatcttcagtagctactggatgcactgggtccgccaagctccagggaaggGggctggtgtgggtctcacgtactaatacgggggggactgacacagcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacggtgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattattgtgcaagagatacggggcagtggaaggactttgactactggggccagggaaccctggtcaccgtctccccagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [286] c86>t,T29>I(- - -)|t167>c,I56>T(- - -)|g173>c,S58>T(+ - +)|t174>g,S58>T(+ - +)|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|c259>g,L87>V(+ - +)|g303>t|c309>t| c86>t,T29>I(- - -)| t167>c,I56>T(- - -)|g173>c,S58>T(+ - +)|t174>g,S58>T(+ - +)|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|c259>g,L87>V(+ - +)|g303>t|c309>t| 320 226 209 17 3 14 3 1 2 1 1 0 0 0 3 3 1 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 16 8 0 8 2 1 0 1 0 0 0 0 2 0 0 2 117 114 106 8 3 5 1 0 1 0 1 0 0 0 1 3 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt actaatacgggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacggtgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattattgt gcaagagatacggggcagtggaaggactttgactac ARDTGQWKDFDY IGHV3-74 IGHD6-19 IGHJ4 ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt actaatacgggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacggtgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattattgt gcaagagatacggggcagtggaaggactttgactac IGA1 17 213 7 10 3 10 4 7 3 0 5 9 JY8QFUQ01BSD92 6153 productive (see comment) Homsap IGHV4-61*02 F 986 94.95 207/218 nt 93.58 204/218 nt Homsap IGHJ6*02 F 202 80.65 50/62 nt Homsap IGHD3-10*01 F 2 9 7 13 9.7.13 [1.17.38.11] CARGSGTYYNPMDVW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 39 of V-REGION: 'ACTG' (4 nucleotides at position 34 to 37 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 64 of V-REGION: 'A' (1 nucleotides at position 105 to 105 in the user submitted sequence), (cause frameshift); in CDR1-IMGT, from codon 37 of V-REGION: 3 nucleotides (from position 30 in the user submitted sequence), (do not cause frameshift) tttctggtgcctccatcagcagtggtagttactACTGgggactggatccggcagcccgccgggaagggactggagtggattgggcgtatccataccagtgggggAcaccaactacaccccctccctcaagagtcgactcaccatatcagtagacgcgtccaagaaccaggtctccctgaggctgagctctgtgaccgccgcagacacggccgtgtattactgtgcgagaggttcggggacttattacaaccctatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaaggggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 94 [295] c75>t|g83>c,G28>A(- + -)|a118>g,S40>D(- - -)|g119>a,S40>D(- - -)|t169>c,Y57>H(+ - -)|a190>g,S64>G(+ + -)|a203>c,N68>T(- + -)|g226>c,V76>L(+ - +)|a244>g,T82>A(- - -)|t259>g,F87>V(+ - -)|a269>g,K90>R(+ + +)| c75>t| g83>c,G28>A(- + -)| a118>g,S40>D(- - -)|g119>a,S40>D(- - -)| t169>c,Y57>H(+ - -)|a190>g,S64>G(+ + -)| a203>c,N68>T(- + -)|g226>c,V76>L(+ - +)|a244>g,T82>A(- - -)|t259>g,F87>V(+ - -)|a269>g,K90>R(+ + +)| a320>g| 319 (320) 225 (226) 214 11 (12) 1 (2) 10 4 (5) 1 1 1 1 0 0 0 2 0 0 1 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 27 26 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 49 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 30 21 19 2 0 2 1 0 0 1 0 0 0 0 0 0 0 0 117 114 109 5 0 5 2 0 0 0 1 0 0 0 1 0 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tttct ggtgcctccatcagcagtggtagttac tgggactggatccggcagcccgccgggaagggactggagtggattgggcgt atccataccagtgggggcacc aactacaccccctccctcaagagtcgactcaccatatcagtagacgcgtccaagaaccaggtctccctgaggctgagctctgtgaccgccgcagacacggccgtgtattactgt gcgagaggttcggggacttattacaaccctatggacgtc ARGSGTYYNPMDV IGHV4-61 IGHD3-10 IGHJ6 ggtgcctccatcagcagtggtagttac tgggactggatccggcagcccgccgggaagggactggagtggattgggcgt atccataccagtgggggcacc aactacaccccctccctcaagagtcgactcaccatatcagtagacgcgtccaagaaccaggtctccctgaggctgagctctgtgaccgccgcagacacggccgtgtattactgt gcgagaggttcggggacttattacaaccctatggacgtc IGG2 10 213 6 4 1 3 5 7 0 0 7 3 JY8QFUQ01BSDSX 6826 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 994 95.83 207/216 nt NA Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [1.17.38.11] CAKDGARGGGSCFNSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) tctggattcacctttagaagctatgccatgagctgggtccgccaggttccagggaaggggctggagtgggtctcagctattagtggtaatggtgctaacacatactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgtgcgaaagatggcgctagaggtggcggcagttgctttaactcctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG1 100 100 [290] c105>a,S35>R(- - -)|c134>t,A45>V(+ - +)|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|g191>a,S64>N(- - -)|g225>a|c248>t,S83>F(- - -)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)| c105>a,S35>R(- - -)| c134>t,A45>V(+ - +)| g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|g191>a,S64>N(- - -)| g225>a|c248>t,S83>F(- - -)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)| 320 224 215 9 1 8 0 3 2 0 1 1 0 0 2 0 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 2 0 0 0 0 0 0 1 0 0 0 117 114 110 4 1 3 0 1 1 0 1 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcacctttagaagctatgcc atgagctgggtccgccaggttccagggaaggggctggagtgggtctcagct attagtggtaatggtgctaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtggcggcagttgctttaactcc AKDGARGGGSCFNS IGHV3-23 IGHD5-24 IGHJ4 ggattcacctttagaagctatgcc atgagctgggtccgccaggttccagggaaggggctggagtgggtctcagct attagtggtaatggtgctaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtggcggcagttgctttaactcc IGG1 9 213 5 4 5 8 0 1 1 0 4 4 JY8QFUQ01BSGWN 15661 productive Homsap IGHV3-48*02 F 939 93.46 200/214 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-16*01 F 3 8 8 13 8.8.13 [0.17.38.11] CARDMIIFGGALDNW in-frame - tggattcgccttcagtagttccagcatgaactgggtccgccagggtccagggaaggggctggagtggatttcacacattaggggtagtagtagtaccacccactacgcagactctgtgaagggccggttcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagacgaggacacggctgtctattactgtgcgagagatatgattatctttgggggagcccttgacaactggggccagggaaccctggtcaccgtctcctctgcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [284] a85>g,T29>A(- - -)|c108>t|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)|c134>g,A45>G(- + -)|g157>a,V53>I(+ - +)|t163>c,Y55>H(+ - -)|t171>g,S57>R(- - -)|a172>g,S58>G(+ + -)|t194>c,I65>T(- - -)|a195>c,I65>T(- - -)|t196>c,Y66>H(+ - -)|a225>g|g303>c| a85>g,T29>A(- - -)|c108>t|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)| c134>g,A45>G(- + -)|g157>a,V53>I(+ - +)|t163>c,Y55>H(+ - -)| t171>g,S57>R(- - -)|a172>g,S58>G(+ + -)|t194>c,I65>T(- - -)|a195>c,I65>T(- - -)| t196>c,Y66>H(+ - -)|a225>g|g303>c| 320 222 208 14 3 11 3 1 1 4 2 0 0 0 1 1 0 1 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 1 1 1 0 0 0 0 0 0 0 51 51 48 3 0 3 0 1 0 1 0 0 0 0 0 1 0 0 30 24 20 4 0 4 1 0 0 1 1 0 0 0 0 0 0 1 117 114 111 3 2 1 1 0 0 1 0 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggattcgccttcagtagttccagc atgaactgggtccgccagggtccagggaaggggctggagtggatttcacac attaggggtagtagtagtaccacc cactacgcagactctgtgaagggccggttcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagacgaggacacggctgtctattactgt gcgagagatatgattatctttgggggagcccttgacaac ARDMIIFGGALDN IGHV3-48 IGHD3-16 IGHJ4 ggattcgccttcagtagttccagc atgaactgggtccgccagggtccagggaaggggctggagtggatttcacac attaggggtagtagtagtaccacc cactacgcagactctgtgaagggccggttcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagacgaggacacggctgtctattactgt gcgagagatatgattatctttgggggagcccttgacaac IGA2 14 213 9 5 2 4 7 10 2 1 4 7 JY8QFUQ01BSOH5 1391 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 931 92.59 200/216 nt 92.13 199/216 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [1.16.38.11] CVRAGFGELSGFDCW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 55 of V-REGION: 1 nucleotides (from position 78 in the user submitted sequence), (cause frameshift) ctctggattcacctttggttattatggcatgagctgggtccgccaagctccggggaggggcgtggagtgggtctctgtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG2 93 88 [287] a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a138>g|a143>g,K48>R(+ + +)|g147>c|c148>g,L50>V(+ - +)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a138>g|a143>g,K48>R(+ + +)|g147>c|c148>g,L50>V(+ - +)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 223 (224) 206 17 (18) 4 (5) 13 3 3 1 1 1 (2) 0 2 0 3 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 50 46 4 2 2 2 0 0 0 0 0 0 0 1 1 0 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ctct ggattcacctttggttattatggc atgagctgggtccgccaagctccggggaggggcgtggagtgggtctctgt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgagctgggtccgccaagctccggggaggggcgtggagtgggtctctgt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG2 16 212 7 9 3 9 4 7 4 0 8 4 JY8QFUQ01BSRSP 18136 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 930 92.99 199/214 nt NA Homsap IGHJ5*02 F 183 84.31 43/51 nt Homsap IGHD4-11*01 ORF 2 8 7 17 8.7.17 [1.17.38.11] CARVGRSVGYSDSGWFDPW in-frame + ctctggtggctccatgagtagttactactggaactggattcggcagcccccagggaagggactggagtggattgggtatatctattacactgggatcaccaactacaatccctccctcaagagtcgagtcaccatgtcaatagacacgtccaggaagcagttctccctgaccctgacctctgtgaccgctgcggacacggccgtctatttctgtgcgagagtcggccggagtgtgggctacagtgactctgggtggttcgacccctggggccagggaaccctggtcaccgtctcctaccatccccgaccagccccaaggtcttccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [294, 295] c90>g,I30>M(+ + -)|g119>a,S40>N(- - -)|c126>t|g176>c,S59>T(+ - +)|g191>t,S64>I(- - -)|c204>t|a234>g,I78>M(+ + -)|g238>a,V80>I(+ - +)|a251>g,K84>R(+ + +)|c255>g,N85>K(+ - -)|a269>c,K90>T(- - -)|g270>c,K90>T(- - -)|g275>c,S92>T(+ - +)|g303>c|a308>t,Y103>F(- + -)| c90>g,I30>M(+ + -)| g119>a,S40>N(- - -)|c126>t| g176>c,S59>T(+ - +)|g191>t,S64>I(- - -)| c204>t|a234>g,I78>M(+ + -)|g238>a,V80>I(+ - +)|a251>g,K84>R(+ + +)|c255>g,N85>K(+ - -)|a269>c,K90>T(- - -)|g270>c,K90>T(- - -)|g275>c,S92>T(+ - +)|g303>c|a308>t,Y103>F(- + -)| a320>t| 319 (320) 221 (222) 206 15 (16) 3 (4) 12 2 2 2 0 1 0 1 (2) 0 4 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 49 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 30 21 19 2 0 2 0 0 0 0 0 0 0 0 1 0 1 0 117 114 104 10 2 8 2 1 1 0 1 0 1 0 3 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtggctccatgagtagttactac tggaactggattcggcagcccccagggaagggactggagtggattgggtat atctattacactgggatcacc aactacaatccctccctcaagagtcgagtcaccatgtcaatagacacgtccaggaagcagttctccctgaccctgacctctgtgaccgctgcggacacggccgtctatttctgt gcgagagtcggccggagtgtgggctacagtgactctgggtggttcgacccc ARVGRSVGYSDSGWFDP IGHV4-59 IGHD4-11 IGHJ5 ggtggctccatgagtagttactac tggaactggattcggcagcccccagggaagggactggagtggattgggtat atctattacactgggatcacc aactacaatccctccctcaagagtcgagtcaccatgtcaatagacacgtccaggaagcagttctccctgaccctgacctctgtgaccgctgcggacacggccgtctatttctgt gcgagagtcggccggagtgtgggctacagtgactctgggtggttcgacccc IGA1 15 210 6 9 4 11 2 4 3 0 9 3 JY8QFUQ01BSZDV 18685 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 942 92.27 203/220 nt NA Homsap IGHJ2*01 F 247 96.23 51/53 nt Homsap IGHD2-21*01 F 1 8 8 12 8.8.12 [2.17.38.11] CAKVVWSLWYFDLW in-frame - agcctctacattcgcctttagcaactatgccatgagctgggtccgccaggctccgagggaagggctggagtgggtgtcagatattagtgggagtggtgttagcacacactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgtgcgaaagtagtctggagcctctggtacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [288] g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)|a138>g|g139>a,G47>R(- - -)|a142>g,K48>E(+ - -)|g144>a,K48>E(+ - -)|c159>g|c164>a,A55>D(- - -)|t174>g|g188>t,G63>V(- - -)|t196>c,Y66>H(+ - -)|a204>g|a214>c,K72>Q(+ - -)|c249>g|a285>t,R95>S(- - -)| g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|a85>g,T29>A(- - -)|g107>a,S36>N(- - -)| a138>g|g139>a,G47>R(- - -)|a142>g,K48>E(+ - -)|g144>a,K48>E(+ - -)|c159>g|c164>a,A55>D(- - -)| t174>g|g188>t,G63>V(- - -)| t196>c,Y66>H(+ - -)|a204>g|a214>c,K72>Q(+ - -)|c249>g|a285>t,R95>S(- - -)| a320>t| 319 (320) 227 (228) 210 17 (18) 5 (6) 12 4 4 0 1 1 1 1 (2) 0 1 2 1 1 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 2 0 0 0 0 0 0 1 0 0 0 51 51 45 6 2 4 2 2 0 0 0 1 0 0 0 1 0 0 30 24 22 2 1 1 0 0 0 0 0 0 0 0 0 0 1 1 117 114 109 5 2 3 1 0 0 1 1 0 1 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct acattcgcctttagcaactatgcc atgagctgggtccgccaggctccgagggaagggctggagtgggtgtcagat attagtgggagtggtgttagcaca cactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgt gcgaaagtagtctggagcctctggtacttcgatctc AKVVWSLWYFDL IGHV3-23 IGHD2-21 IGHJ2 acattcgcctttagcaactatgcc atgagctgggtccgccaggctccgagggaagggctggagtgggtgtcagat attagtgggagtggtgttagcaca cactacgcggactccgtgcagggccggttcaccatctccagagacaattcgaagaacacgctgtatctgcaaatgaacagcctgagtgccgaggacacggccgtatattactgt gcgaaagtagtctggagcctctggtacttcgatctc IGG1 17 213 9 8 4 9 5 8 4 1 7 5 JY8QFUQ01BT5A6 2432 productive (see comment) Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 909 91.24 198/217 nt 90.78 197/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVRAGFGELSGFDCW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 53 of V-REGION: 'GTC' (3 nucleotides at position 72 to 74 in the user submitted sequence), (do not cause frameshift); ctctggattcacctttggttattatggcatgagctgggtccgccaagctccggggaggggctgggagtgggGTCagtctggtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [291] a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a138>g|a143>g,K48>R(+ + +)|g147>c|c148>t,L50>W(+ - -)|t149>g,L50>W(+ - -)|t158>a,V53>E(- + -)|c159>g,V53>E(- + -)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a138>g|a143>g,K48>R(+ + +)|g147>c|c148>t,L50>W(+ - -)|t149>g,L50>W(+ - -)|t158>a,V53>E(- + -)|c159>g,V53>E(- + -)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 224 (225) 204 20 (21) 4 (5) 16 3 3 2 1 1 (2) 0 2 1 3 2 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 51 44 7 2 5 2 0 1 0 0 0 0 1 1 1 0 1 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ctct ggattcacctttggttattatggc atgagctgggtccgccaagctccggggaggggctgggagtgggagtctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgagctgggtccgccaagctccggggaggggctgggagtgggagtctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 19 213 8 11 4 10 4 9 4 0 11 4 JY8QFUQ01BT5S9 11054 productive Homsap IGHV4-39*02 F 983 94.12 208/221 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 3 10 7 10 10.7.10 [1.17.38.11] CARIVVRQFDQW in-frame + tctctggtgactccatttccagtactagttattactggggctgggtccgccagcccccagggaaggggctggagtggattgggggtatctattctagtgggaccacctactacaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaacaactacttctccctgaagctgagttctgtgaccgccgcagacacggctgtgtattactgtgcgagaatagtggttagacagtttgaccagtggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [282] g83>a,G28>D(- - -)|c90>t|a91>t|g92>c|g104>c,S35>T(+ - +)|c111>t|a124>g,I42>V(+ - +)|a163>g,S55>G(+ + -)|a173>c,Y58>S(+ - -)|g191>c,S64>T(+ - +)|g252>c,K84>N(+ - -)|c256>t,H86>Y(+ - -)|c276>t| g83>a,G28>D(- - -)|c90>t|a91>t|g92>c|g104>c,S35>T(+ - +)|c111>t| a124>g,I42>V(+ - +)|a163>g,S55>G(+ + -)| a173>c,Y58>S(+ - -)|g191>c,S64>T(+ - +)| g252>c,K84>N(+ - -)|c256>t,H86>Y(+ - -)|c276>t| g319>a|a320>t| 318 (320) 227 (229) 214 13 (15) 5 (7) 8 2 1 (2) 4 0 1 0 1 (2) 0 4 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 4 2 0 1 2 0 0 0 1 0 2 0 0 0 51 51 49 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 30 21 19 2 0 2 0 0 0 0 1 0 0 0 1 0 0 0 117 114 111 3 1 2 0 0 2 0 0 0 0 0 1 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatttccagtactagttattac tggggctgggtccgccagcccccagggaaggggctggagtggattgggggt atctattctagtgggaccacc tactacaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaacaactacttctccctgaagctgagttctgtgaccgccgcagacacggctgtgtattactgt gcgagaatagtggttagacagtttgaccag ARIVVRQFDQ IGHV4-39 IGHD3-22 IGHJ4 ggtgactccatttccagtactagttattac tggggctgggtccgccagcccccagggaaggggctggagtggattgggggt atctattctagtgggaccacc tactacaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaacaactacttctccctgaagctgagttctgtgaccgccgcagacacggctgtgtattactgt gcgagaatagtggttagacagtttgaccag IGA1 13 216 7 6 5 9 2 4 1 4 4 4 JY8QFUQ01BTAHR 21423 productive (see comment) Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 878 89.45 195/218 nt 88.99 194/218 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARKRVPGGTFYGDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 61 to 61 in the user submitted sequence), (cause frameshift); cctctggattcacctttagtacctatggcatgctctgggtccgccatgttgcaggcaaggGggctggagtgggtggcaactatatcagctgatggacgaaataaatactatgcagattccgtgatgggccgattcgccctctccagagacaaatccaagaacacggattatctgcaaatgaacagcctgagaactgacgacacggctgtatattactgtgcgagaaagagagtccccggggggactttttatggggactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccccctggcgccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccgggtgacggtgtcgtggaactca NA IGG1 86 100 [294, 293] c90>t|g107>c,S36>T(+ - +)|a119>t,H40>L(- - -)|g132>t,Q44>H(- + -)|c134>t,A45>V(+ - +)|c136>g,P46>A(- - -)|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)|t172>g,Y58>A(- - -)|a173>c,Y58>A(- - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)|c207>t|a215>t,K72>M(- + -)|a229>g,T77>A(- - -)|a232>c,I78>L(+ + +)|t246>a,N82>K(+ - -)|c259>g,L87>D(- - -)|t260>a,L87>D(- - -)|g261>t,L87>D(- - -)|g286>a,A96>T(- - -)|g291>c,E97>D(+ - +)|g303>a| c90>t|g107>c,S36>T(+ - +)| a119>t,H40>L(- - -)|g132>t,Q44>H(- + -)|c134>t,A45>V(+ - +)|c136>g,P46>A(- - -)|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)| t172>g,Y58>A(- - -)|a173>c,Y58>A(- - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)| c207>t|a215>t,K72>M(- + -)|a229>g,T77>A(- - -)|a232>c,I78>L(+ + +)|t246>a,N82>K(+ - -)|c259>g,L87>D(- - -)|t260>a,L87>D(- - -)|g261>t,L87>D(- - -)|g286>a,A96>T(- - -)|g291>c,E97>D(+ - +)|g303>a| g319>a| 318 (320) 224 (226) 201 (202) 23 (24) 3 (4) 20 1 3 (4) 3 1 3 0 2 3 2 2 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 1 0 0 0 0 0 1 0 0 0 51 51 45 6 0 6 0 1 1 1 0 0 1 0 0 1 1 0 30 24 20 4 0 4 0 0 0 0 2 0 0 1 0 0 0 1 117 114 103 11 2 9 1 2 1 0 1 0 1 2 1 1 1 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagtacctatggc atgctctgggtccgccatgttgcaggcaaggggctggagtgggtggcaact atatcagctgatggacgaaataaa tactatgcagattccgtgatgggccgattcgccctctccagagacaaatccaagaacacggattatctgcaaatgaacagcctgagaactgacgacacggctgtatattactgt gcgagaaagagagtccccggggggactttttatggggactac ARKRVPGGTFYGDY IGHV3-30 IGHD3-10 IGHJ4 ggattcacctttagtacctatggc atgctctgggtccgccatgttgcaggcaaggggctggagtgggtggcaact atatcagctgatggacgaaataaa tactatgcagattccgtgatgggccgattcgccctctccagagacaaatccaagaacacggattatctgcaaatgaacagcctgagaactgacgacacggctgtatattactgt gcgagaaagagagtccccggggggactttttatggggactac IGG1 23 213 8 15 6 12 2 11 2 1 15 5 JY8QFUQ01BTCL3 4594 productive Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 999 95.85 208/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-16*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARGPAGGSGAYEYW in-frame + ctctggattcaagtttgatgattccggcatgagctgggtccgccaagctccagggaaggggctggagtgggtctctggtattaattggaatggtggtaagacaggttatggagactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatttgcaaatgaacagtctgagagccgaggacacggccttgtattactgtgcgagaggccccgccgggggatctggcgcatatgagtactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgcntgacgcctcgacagcacccccccaagatgggaacgtggtccgtcgcatgcctggtccagggcttcttcc NA IGA2 71 79 [287, 289, 290] c86>a,T29>K(- - -)|c87>g,T29>K(- - -)|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)|g191>a,S64>K(- - -)|c192>g,S64>K(- - -)|c203>g,A68>G(- + -)|c265>t|c307>t,H103>Y(+ - -)| c86>a,T29>K(- - -)|c87>g,T29>K(- - -)|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)| g191>a,S64>K(- - -)|c192>g,S64>K(- - -)| c203>g,A68>G(- + -)|c265>t|c307>t,H103>Y(+ - -)| a320>g| 319 (320) 224 (225) 215 9 (10) 1 (2) 8 0 (1) 1 2 1 1 1 0 0 0 3 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 1 1 1 0 0 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 0 1 0 0 117 114 111 3 1 2 0 0 2 0 0 0 0 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ctct ggattcaagtttgatgattccggc atgagctgggtccgccaagctccagggaaggggctggagtgggtctctggt attaattggaatggtggtaagaca ggttatggagactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatttgcaaatgaacagtctgagagccgaggacacggccttgtattactgt gcgagaggccccgccgggggatctggcgcatatgagtac ARGPAGGSGAYEY IGHV3-20 IGHD3-16 IGHJ4 ggattcaagtttgatgattccggc atgagctgggtccgccaagctccagggaaggggctggagtgggtctctggt attaattggaatggtggtaagaca ggttatggagactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatttgcaaatgaacagtctgagagccgaggacacggccttgtattactgt gcgagaggccccgccgggggatctggcgcatatgagtac IGA2 9 213 4 5 3 7 1 2 1 0 2 6 JY8QFUQ01BTIAT 11656 productive Homsap IGHV3-9*01 F 922 92.13 199/216 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 2 8 8 16 8.8.16 [1.17.38.11] CAKDGSAIPVTGTYFEYW in-frame + tctggattcacctttcatgattataccatgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagtattagttggaatagtggtaacatagactatgcggcctctgtgaggggccgattcaccgtctccagagacaacgccaataactccctgtctcttcagatgaatggtctgagatctgaggacacggccctctattactgtgcaaaagacgggtcggcgataccagtgactggtacctactttgaatactggggccagggagccctggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcag NA IGG3 100 88 [296] g103>c,D35>H(- - -)|g112>a,A38>T(- - -)|g163>a,G55>S(+ + -)|g191>a,S64>N(- - -)|g197>a,G66>D(- - -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|g252>t,K84>N(+ - -)|a263>c,Y88>S(+ - -)|g267>t|a270>g|c276>t|a277>g,S93>G(+ + -)|g286>t,A96>S(- + -)|t301>c|g303>c| g103>c,D35>H(- - -)|g112>a,A38>T(- - -)| g163>a,G55>S(+ + -)| g191>a,S64>N(- - -)| g197>a,G66>D(- - -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|g252>t,K84>N(+ - -)|a263>c,Y88>S(+ - -)|g267>t|a270>g|c276>t|a277>g,S93>G(+ + -)|g286>t,A96>S(- + -)|t301>c|g303>c| t321>c|a322>g| 320 (322) 224 (226) 207 17 (19) 5 (7) 12 4 (5) 4 1 1 (2) 2 0 0 0 2 0 3 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 117 114 101 13 5 8 4 1 1 1 2 0 0 0 1 0 3 0 8 (10) 8 8 0 (2) 0 (2) 0 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| tct ggattcacctttcatgattatacc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt attagttggaatagtggtaacata gactatgcggcctctgtgaggggccgattcaccgtctccagagacaacgccaataactccctgtctcttcagatgaatggtctgagatctgaggacacggccctctattactgt gcaaaagacgggtcggcgataccagtgactggtacctactttgaatac AKDGSAIPVTGTYFEY IGHV3-9 IGHD6-19 IGHJ4 ggattcacctttcatgattatacc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaagt attagttggaatagtggtaacata gactatgcggcctctgtgaggggccgattcaccgtctccagagacaacgccaataactccctgtctcttcagatgaatggtctgagatctgaggacacggccctctattactgt gcaaaagacgggtcggcgataccagtgactggtacctactttgaatac IGG3 17 213 10 7 5 10 5 7 5 0 9 3 JY8QFUQ01BTOR9 15731 productive (see comment) Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F or Homsap IGHV3-15*07 F 892 89.95 197/219 nt 89.5 196/219 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD5-24*01 ORF 1 8 10 10 8.10.10 [X.17.38.11] CTHLREEMSYEW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 95 of V-REGION: 'A' (1 nucleotides at position 190 to 190 in the user submitted sequence), (cause frameshift); tggagtcaattcagaaacgcctggatgaattgggtccgccaggctccagggaaggggctggagtgggttggccgtattaagagcaaagctgatggtgggacaacagactacgccacacccgtgagaggcagattcaccatctcaagagatgattcaaaaaacacgttttatctgcaaatgaatagcctaAaaaaccgaagacacagccgtctattactgtacccacttgcgagaagagatgtcttacgaatggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [281] g79>t,G27>W(- - -)|a81>g,G27>W(- - -)|t82>a,F28>S(- - -)|t83>g,F28>S(- - -)|c84>t,F28>S(- - -)|a85>c,T29>Q(- - -)|c86>a,T29>Q(- - -)|t87>a,T29>Q(- - -)|t105>a,S35>R(- - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a171>g|a178>g,T60>A(- - -)|t204>c|g205>a,A69>T(- - -)|a215>g,K72>R(+ + +)|c259>t,L87>F(+ - -)|g261>t,L87>F(+ - -)|c276>t|g282>a|g291>a|g303>c| g79>t,G27>W(- - -)|a81>g,G27>W(- - -)|t82>a,F28>S(- - -)|t83>g,F28>S(- - -)|c84>t,F28>S(- - -)|a85>c,T29>Q(- - -)|c86>a,T29>Q(- - -)|t87>a,T29>Q(- - -)|t105>a,S35>R(- - -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)| a171>g|a178>g,T60>A(- - -)| t204>c|g205>a,A69>T(- - -)|a215>g,K72>R(+ + +)|c259>t,L87>F(+ - -)|g261>t,L87>F(+ - -)|c276>t|g282>a|g291>a|g303>c| a316>c,T106>H(+ - -)|c317>a,T106>H(+ - -)|a318>c,T106>H(+ - -)|g319>t|a320>t| 315 (320) 222 (227) 200 22 (27) 6 (8) 16 (19) 4 4 4 1 1 (3) 1 (2) 0 (1) 3 1 0 2 (3) 1 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 15 9 0 9 1 0 1 0 1 1 0 3 0 0 1 1 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 30 28 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 117 114 105 9 5 4 1 3 2 1 0 0 0 0 1 0 1 0 3 (8) 3 3 0 (5) 0 (2) 0 (3) 0 0 0 0 0 (2) 0 (1) 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| tggagtcaattcagaaacgcctgg atgaattgggtccgccaggctccagggaaggggctggagtgggttggccgt attaagagcaaagctgatggtgggacaaca gactacgccacacccgtgagaggcagattcaccatctcaagagatgattcaaaaaacacgttttatctgcaaatgaatagcctaaaaaccgaagacacagccgtctattactgt acccacttgcgagaagagatgtcttacgaa THLREEMSYE IGHV3-15 IGHD5-24 IGHJ4 tggagtcaattcagaaacgcctgg atgaattgggtccgccaggctccagggaaggggctggagtgggttggccgt attaagagcaaagctgatggtgggacaaca gactacgccacacccgtgagaggcagattcaccatctcaagagatgattcaaaaaacacgttttatctgcaaatgaatagcctaaaaaccgaagacacagccgtctattactgt acccacttgcgagaagagatgtcttacgaa IGA2 22 219 13 9 8 12 5 10 5 1 6 10 JY8QFUQ01BU31Q 12273 productive (see comment) Homsap IGHV3-9*01 F 891 90.32 196/217 nt 89.86 195/217 nt Homsap IGHJ2*01 F 247 96.23 51/53 nt Homsap IGHD3-22*01 F 2 8 8 22 8.8.22 [1.17.38.11] CVRQAYYHDSSAYFFSRYWYFDLW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 60 to 60 in the user submitted sequence), (cause frameshift); ctctggattcacttttgatgactctgccatgcactgggtgcggcaagctccagggaaggGgcccggagtgggtcgcaggtattagtggaaatagtggaaatataggatatgcggactcagtgaagggccgatgcaccatctccagagacaacgccaagaagtccctgtttctgcaaattaaaagtctgagagttgaggacacggccttatattattgtgtgaggcaagcgtattaccatgatagcagtgcttacttcttctcccgctactggtacttcgatctctggggccgtggcaccccggtcactgtctcttcagcctccaccaaggggcccatcggtcttccccctggcaccctcctccaagagcacctctggggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 100 94 [317] c87>t|t108>c|a110>c,Y37>S(+ - -)|c126>g|t149>c,L50>P(- - -)|t160>g,S54>A(- + -)|t172>g,W58>G(- - -)|g174>a,W58>G(- - -)|t189>a|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)|c198>a|t210>a|t227>g,F76>C(+ - -)|c255>g,N85>K(+ - -)|a263>t,Y88>F(- + -)|g273>t,M91>I(+ + -)|c276>a,N92>K(+ - -)|c287>t,A96>V(+ - +)|g303>a|c309>t| c87>t|t108>c|a110>c,Y37>S(+ - -)| c126>g|t149>c,L50>P(- - -)|t160>g,S54>A(- + -)| t172>g,W58>G(- - -)|g174>a,W58>G(- - -)|t189>a|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)| c198>a|t210>a|t227>g,F76>C(+ - -)|c255>g,N85>K(+ - -)|a263>t,Y88>F(- + -)|g273>t,M91>I(+ + -)|c276>a,N92>K(+ - -)|c287>t,A96>V(+ - +)|g303>a|c309>t| c314>t,A105>V(+ - +)|a315>g,A105>V(+ - +)|a317>g,K106>R(+ + +)|a318>g,K106>R(+ + +)|g319>c,D107>Q(+ - -)|t321>a,D107>Q(+ - -)|a322>g| 313 (322) 218 (227) 197 (199) 21 (28) 8 (9) 13 (19) 0 (4) 3 4 (5) 2 1 2 1 2 (3) 0 (1) 2 1 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 1 1 1 0 0 0 0 0 0 0 51 51 48 3 1 2 0 0 0 1 0 0 0 0 0 1 0 1 30 24 19 5 1 4 0 2 1 0 0 0 0 1 0 0 0 1 117 114 104 10 4 6 0 1 2 0 0 2 1 1 0 1 1 1 1 (10) 1 1 (3) 0 (7) 0 (1) 0 (6) 0 (4) 0 0 (1) 0 0 0 0 0 (1) 0 (1) 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacttttgatgactctgcc atgcactgggtgcggcaagctccagggaagggcccggagtgggtcgcaggt attagtggaaatagtggaaatata ggatatgcggactcagtgaagggccgatgcaccatctccagagacaacgccaagaagtccctgtttctgcaaattaaaagtctgagagttgaggacacggccttatattattgt gtgaggcaagcgtattaccatgatagcagtgcttacttcttctcccgctactggtacttcgatctc VRQAYYHDSSAYFFSRYWYFDL IGHV3-9 IGHD3-22 IGHJ2 ggattcacttttgatgactctgcc atgcactgggtgcggcaagctccagggaagggcccggagtgggtcgcaggt attagtggaaatagtggaaatata ggatatgcggactcagtgaagggccgatgcaccatctccagagacaacgccaagaagtccctgtttctgcaaattaaaagtctgagagttgaggacacggccttatattattgt gtgaggcaagcgtattaccatgatagcagtgcttacttcttctcccgctactggtacttcgatctc IGG1 21 213 9 12 7 12 2 9 5 3 8 5 JY8QFUQ01BU3DY 12971 productive Homsap IGHV3-11*01 F 981 94.93 206/217 nt NA Homsap IGHJ6*01 F 252 88.89 56/63 nt Homsap IGHD1-26*01 F 3 8 8 15 8.8.15 [1.17.38.11] CVRYWGSYYYYNGLDVW in-frame + ctctggattcaccttcactgactactacatgacctggatccgccaggctccagggaaggggctggagtgggtttcacacattagtagtggtggtagaaccattgcctacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcagtgtttctgcaaatgaacagcctgagagccgaggacacggccgtgtattattgtgtgagatattggggcagctactattactataacggtctggacgtctgggggcaagggaccacggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcctgcctggtccagggcttcttccccca NA IGA2 86 100 [293] g104>c,S35>T(+ - +)|g119>c,S40>T(+ - +)|t163>c,Y55>H(+ - -)|a175>g,S59>G(+ + -)|t189>a,S63>R(- - -)|a195>t|t196>g,Y66>A(- - -)|a197>c,Y66>A(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c309>t|c314>t,A105>V(+ - +)| g104>c,S35>T(+ - +)| g119>c,S40>T(+ - +)|t163>c,Y55>H(+ - -)| a175>g,S59>G(+ + -)|t189>a,S63>R(- - -)|a195>t| t196>g,Y66>A(- - -)|a197>c,Y66>A(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c309>t| c314>t,A105>V(+ - +)|g319>t| 318 (320) 223 (225) 211 (212) 12 (13) 2 (3) 10 1 0 2 1 1 0 2 1 2 1 0 (1) 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 49 2 0 2 0 0 0 1 0 0 0 0 1 0 0 0 30 24 21 3 1 2 1 0 0 0 0 0 1 1 0 0 0 0 117 114 109 5 1 4 0 0 1 0 1 0 1 0 0 1 0 1 6 (8) 6 5 (6) 1 (2) 0 (1) 1 0 0 1 0 0 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcactgactactac atgacctggatccgccaggctccagggaaggggctggagtgggtttcacac attagtagtggtggtagaaccatt gcctacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcagtgtttctgcaaatgaacagcctgagagccgaggacacggccgtgtattattgt gtgagatattggggcagctactattactataacggtctggacgtc VRYWGSYYYYNGLDV IGHV3-11 IGHD1-26 IGHJ6 ggattcaccttcactgactactac atgacctggatccgccaggctccagggaaggggctggagtgggtttcacac attagtagtggtggtagaaccatt gcctacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcagtgtttctgcaaatgaacagcctgagagccgaggacacggccgtgtattattgt gtgagatattggggcagctactattactataacggtctggacgtc IGA2 11 213 3 8 1 4 2 7 1 1 6 3 JY8QFUQ01BU5NL 774 productive Homsap IGHV3-66*02 F 858 89.25 191/214 nt NA Homsap IGHJ1*01 F 201 86.54 45/52 nt Homsap IGHD6-19*01 F 2 8 7 13 8.7.13 [1.17.38.11] CAAVGVGSAVSFQYW in-frame + ctctggcttcaccgtcaataacaactacatgggctgggtccgccaggctccagggaaggggctggagtgggtctcgattatttattacggtggaaccacatattacgctgactccgtgaagggccgattcaccatctctagagacacctccaagaacacgttatttcttcaaatgaataccctaagaggtgaggacacggctgtgtactactgtgcggcagtcggagtgggaagtgcagtctccttccaatactggggccagggcaccctggtcaccgtctcctnagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcaccccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccaa NA IGA1 79 95 [284, 285] a81>c|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|a118>g,S40>G(+ + -)|a162>g|g163>a,V55>I(+ - +)|a172>t,S58>Y(+ - -)|g173>a,S58>Y(+ - -)|t189>a|g191>c,S64>T(+ - +)|c198>t|a204>t|c237>t|a245>c,N82>T(- + -)|t246>c,N82>T(- + -)|c259>t|g261>a|a263>t,Y88>F(- + -)|c276>t|g278>c,S93>T(+ - +)|g282>a|c287>g,A96>G(- + -)|t306>c| a81>c|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)| a118>g,S40>G(+ + -)|a162>g|g163>a,V55>I(+ - +)| a172>t,S58>Y(+ - -)|g173>a,S58>Y(+ - -)|t189>a|g191>c,S64>T(+ - +)| c198>t|a204>t|c237>t|a245>c,N82>T(- + -)|t246>c,N82>T(- + -)|c259>t|g261>a|a263>t,Y88>F(- + -)|c276>t|g278>c,S93>T(+ - +)|g282>a|c287>g,A96>G(- + -)|t306>c| a316>g,R106>A(- - -)|g317>c,R106>A(- - -)| 315 (318) 217 (220) 194 (195) 23 (25) 11 12 (14) 2 (3) 6 4 2 2 0 3 1 2 (3) 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 2 0 0 1 0 0 0 0 0 0 0 51 51 48 3 1 2 2 1 0 0 0 0 0 0 0 0 0 0 30 21 17 4 1 3 0 1 0 0 0 0 1 1 1 0 0 0 117 114 101 13 8 5 0 2 4 2 1 0 2 0 1 1 0 0 3 (6) 3 3 (4) 0 (2) 0 0 (2) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggcttcaccgtcaataacaactac atgggctgggtccgccaggctccagggaaggggctggagtgggtctcgatt atttattacggtggaaccaca tattacgctgactccgtgaagggccgattcaccatctctagagacacctccaagaacacgttatttcttcaaatgaataccctaagaggtgaggacacggctgtgtactactgt gcggcagtcggagtgggaagtgcagtctccttccaatac AAVGVGSAVSFQY IGHV3-66 IGHD6-19 IGHJ1 ggcttcaccgtcaataacaactac atgggctgggtccgccaggctccagggaaggggctggagtgggtctcgatt atttattacggtggaaccaca tattacgctgactccgtgaagggccgattcaccatctctagagacacctccaagaacacgttatttcttcaaatgaataccctaagaggtgaggacacggctgtgtactactgt gcggcagtcggagtgggaagtgcagtctccttccaatac IGA1 23 210 14 9 10 13 4 10 9 2 7 5 JY8QFUQ01BUAPX 19483 productive Homsap IGHV3-48*02 F, or Homsap IGHV3-48*04 F 801 85.71 186/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-21*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAKVGNLYFFEYW in-frame - ctctggattcagcttcaacagctacagcatgaactgggtccgccaggctccagggaagggactggaatggatctcatcaattagtaccgctggcaccaccataggctacgcagactctgtgaagggccgattcactatttccagagacaacgccaagaactcagtatctctgcagatggacagcctgagagacgaggacacggcggtatattattgtgccaaagtgggtaatttgtatttctttgaatactggggccggggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [281] c86>g,T29>S(+ - +)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|t111>c|g147>a|g153>a|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|a164>c,Y55>S(+ - -)|c165>a,Y55>S(+ - -)|g173>c,S58>T(+ - +)|t174>c,S58>T(+ - +)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|a184>g,S62>G(+ + -)|t186>c,S62>G(+ + -)|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)|t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c231>t|c234>t|t246>c|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a263>c,Y88>S(+ - -)|a270>g|a274>g,N92>D(+ + -)|t300>g|g303>a|c309>t|g315>c|g317>a,R106>K(+ + +)| c86>g,T29>S(+ - +)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|t111>c| g147>a|g153>a|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|a164>c,Y55>S(+ - -)|c165>a,Y55>S(+ - -)| g173>c,S58>T(+ - +)|t174>c,S58>T(+ - +)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|a184>g,S62>G(+ + -)|t186>c,S62>G(+ + -)|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)| t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c231>t|c234>t|t246>c|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a263>c,Y88>S(+ - -)|a270>g|a274>g,N92>D(+ + -)|t300>g|g303>a|c309>t| g315>c|g317>a,R106>K(+ + +)|a320>t| 319 (320) 224 (225) 191 33 (34) 11 (12) 22 5 7 3 7 2 1 0 (1) 0 4 2 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 0 2 0 0 0 0 0 1 0 0 51 51 45 6 2 4 0 3 0 1 1 1 0 0 0 0 0 0 30 24 16 8 0 8 2 0 0 3 0 0 0 0 3 0 0 0 117 114 101 13 7 6 3 2 3 1 1 0 0 0 0 1 0 2 7 (8) 7 5 2 (3) 1 (2) 1 0 1 0 0 0 0 0 (1) 0 1 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ctct ggattcagcttcaacagctacagc atgaactgggtccgccaggctccagggaagggactggaatggatctcatca attagtaccgctggcaccaccata ggctacgcagactctgtgaagggccgattcactatttccagagacaacgccaagaactcagtatctctgcagatggacagcctgagagacgaggacacggcggtatattattgt gccaaagtgggtaatttgtatttctttgaatac AKVGNLYFFEY IGHV3-48 IGHD2-21 IGHJ4 ggattcagcttcaacagctacagc atgaactgggtccgccaggctccagggaagggactggaatggatctcatca attagtaccgctggcaccaccata ggctacgcagactctgtgaagggccgattcactatttccagagacaacgccaagaactcagtatctctgcagatggacagcctgagagacgaggacacggcggtatattattgt gccaaagtgggtaatttgtatttctttgaatac IGA1 31 213 21 10 9 15 12 16 9 1 10 11 JY8QFUQ01BUG1S 16687 productive (see comment) Homsap IGHV1-2*02 F 930 92.99 199/214 nt 92.52 198/214 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-13*01 F 3 8 8 11 8.8.11 [0.17.38.11] CARGQQLRDFDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 57 of V-REGION: 'AACCCC' (6 nucleotides at position 80 to 85 in the user submitted sequence), (do not cause frameshift); tggatacaccttcaccgtctactatctattctgggtgcgacgggcccctggacaagggcttgagtggatgggatggatcAACCCCaaccctaagagtggtgacacacactatgcaccgaaattccagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggaactgaataggctgagatctgacgacacggccgtgtattactgtgcgaggggacagcagcttcgagactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] g107>t,G36>V(- - -)|a115>c,M39>L(+ + -)|g117>a,M39>L(+ + -)|c118>t,H40>F(- - -)|a119>t,H40>F(- - -)|a131>g,Q44>R(+ - -)|c177>g,N59>K(+ - -)|g191>a,G64>D(- - -)|a196>c,N66>H(- - -)|a206>c,Q69>P(- - -)|g210>a|t213>c|g270>a|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)| g107>t,G36>V(- - -)| a115>c,M39>L(+ + -)|g117>a,M39>L(+ + -)|c118>t,H40>F(- - -)|a119>t,H40>F(- - -)|a131>g,Q44>R(+ - -)| c177>g,N59>K(+ - -)|g191>a,G64>D(- - -)| a196>c,N66>H(- - -)|a206>c,Q69>P(- - -)|g210>a|t213>c|g270>a|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)| a318>g|a320>g| 317 (320) 219 (222) 204 (205) 15 (17) 3 (5) 12 1 (3) 5 2 1 3 0 1 0 0 1 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 51 51 46 5 0 5 1 1 1 0 1 0 1 0 0 0 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 0 1 0 0 117 114 107 7 3 4 0 3 1 1 2 0 0 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatacaccttcaccgtctactat ctattctgggtgcgacgggcccctggacaagggcttgagtggatgggatgg atcaaccctaagagtggtgacaca cactatgcaccgaaattccagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggaactgaataggctgagatctgacgacacggccgtgtattactgt gcgaggggacagcagcttcgagactttgactac ARGQQLRDFDY IGHV1-2 IGHD6-13 IGHJ4 ggatacaccttcaccgtctactat ctattctgggtgcgacgggcccctggacaagggcttgagtggatgggatgg atcaaccctaagagtggtgacaca cactatgcaccgaaattccagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggaactgaataggctgagatctgacgacacggccgtgtattactgt gcgaggggacagcagcttcgagactttgactac IGA1 15 213 9 6 7 9 2 6 3 0 9 3 JY8QFUQ01BUJ4F 15156 productive (see comment) Homsap IGHV3-66*02 F 858 89.25 191/214 nt 88.79 190/214 nt Homsap IGHJ1*01 F 206 88.46 46/52 nt Homsap IGHD6-19*01 F 2 8 7 13 8.7.13 [1.17.38.11] CAAVGVGSAVSFQYW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 63 of V-REGION: 'G' (1 nucleotides at position 93 to 93 in the user submitted sequence), (cause frameshift); ctctggcttcaccgtcaataacaactacatgggctgggtccgccaggctccagggaaggggctggagtgggtctcgattatttattacggtgGgaaccacatattacgctgactccgtgaagggccgattcaccatctctagagacacctccaagaacacgttatttcttcaaatgaataccctaagaggtgaggacacggctgtgtactactgtgcggcagtcggagtgggaagtgcagtctccttccaatactggggccagggcaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 89 [285, 284] a81>c|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|a118>g,S40>G(+ + -)|a162>g|g163>a,V55>I(+ - +)|a172>t,S58>Y(+ - -)|g173>a,S58>Y(+ - -)|t189>a|g191>c,S64>T(+ - +)|c198>t|a204>t|c237>t|a245>c,N82>T(- + -)|t246>c,N82>T(- + -)|c259>t|g261>a|a263>t,Y88>F(- + -)|c276>t|g278>c,S93>T(+ - +)|g282>a|c287>g,A96>G(- + -)|t306>c| a81>c|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)| a118>g,S40>G(+ + -)|a162>g|g163>a,V55>I(+ - +)| a172>t,S58>Y(+ - -)|g173>a,S58>Y(+ - -)|t189>a|g191>c,S64>T(+ - +)| c198>t|a204>t|c237>t|a245>c,N82>T(- + -)|t246>c,N82>T(- + -)|c259>t|g261>a|a263>t,Y88>F(- + -)|c276>t|g278>c,S93>T(+ - +)|g282>a|c287>g,A96>G(- + -)|t306>c| a316>g,R106>A(- - -)|g317>c,R106>A(- - -)| 315 (318) 217 (220) 194 (195) 23 (25) 11 12 (14) 2 (3) 6 4 2 2 0 3 1 2 (3) 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 2 0 0 1 0 0 0 0 0 0 0 51 51 48 3 1 2 2 1 0 0 0 0 0 0 0 0 0 0 30 21 17 4 1 3 0 1 0 0 0 0 1 1 1 0 0 0 117 114 101 13 8 5 0 2 4 2 1 0 2 0 1 1 0 0 3 (6) 3 3 (4) 0 (2) 0 0 (2) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggcttcaccgtcaataacaactac atgggctgggtccgccaggctccagggaaggggctggagtgggtctcgatt atttattacggtggaaccaca tattacgctgactccgtgaagggccgattcaccatctctagagacacctccaagaacacgttatttcttcaaatgaataccctaagaggtgaggacacggctgtgtactactgt gcggcagtcggagtgggaagtgcagtctccttccaatac AAVGVGSAVSFQY IGHV3-66 IGHD6-19 IGHJ1 ggcttcaccgtcaataacaactac atgggctgggtccgccaggctccagggaaggggctggagtgggtctcgatt atttattacggtggaaccaca tattacgctgactccgtgaagggccgattcaccatctctagagacacctccaagaacacgttatttcttcaaatgaataccctaagaggtgaggacacggctgtgtactactgt gcggcagtcggagtgggaagtgcagtctccttccaatac IGA2 23 210 14 9 10 13 4 10 9 2 7 5 JY8QFUQ01BUOZE 2124 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 863 89.3 192/215 nt 88.84 191/215 nt Homsap IGHJ3*01 F (see comment) 169 82 41/50 nt Homsap IGHD2-15*01 F 2 7 8 14 7.8.14 [1.17.38.11] CARGAGAEGKGGMDVW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT, from codon 36 of V-REGION: 1 nucleotides (from position 21 in the user submitted sequence), (cause frameshift) tctggattcaccttcaaaagtatggcatgaactggctccgccaggctccagggaaggggctggagtgggtcgcaaccattcgcagtagtggtacttccatacactatgccgactccgtgaagggccgattcactatcaccagagacaacgccaacaactcactgtatctgcaattgaacagcctgggagtcgaggactcggctgtgtatttctgtgcgagaggggccggcgcagaaggtaaaggtggtatggacgtctggggccaagggactacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccag NA IGA1 100 100 [288] g104>a,S35>K(- - -)|t105>a,S35>K(- - -)|a112>g,S38>G(+ + -)|g124>c,V42>L(+ - +)|t160>g,S54>A(- + -)|t163>a,S55>T(+ - +)|a169>c,S57>R(- - -)|t171>c,S57>R(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|a191>c,Y64>S(+ - -)|t196>c,Y66>H(+ - -)|c201>t|a204>c|a210>c|c231>t|t235>a,S79>T(+ - +)|g252>c,K84>N(+ - -)|a271>t,M91>L(+ + -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|a295>t,T99>S(+ - +)|a308>t,Y103>F(- + -)| g104>a,S35>K(- - -)|t105>a,S35>K(- - -)|a112>g,S38>G(+ + -)| g124>c,V42>L(+ - +)|t160>g,S54>A(- + -)|t163>a,S55>T(+ - +)| a169>c,S57>R(- - -)|t171>c,S57>R(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|a191>c,Y64>S(+ - -)| t196>c,Y66>H(+ - -)|c201>t|a204>c|a210>c|c231>t|t235>a,S79>T(+ - +)|g252>c,K84>N(+ - -)|a271>t,M91>L(+ + -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|a295>t,T99>S(+ - +)|a308>t,Y103>F(- + -)| a320>g| 319 (320) 222 (223) 199 23 (24) 4 (5) 19 3 (4) 1 3 2 4 0 3 3 3 0 0 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 20 3 0 3 1 1 0 0 0 0 0 1 0 0 0 0 51 51 48 3 0 3 0 0 0 0 0 0 0 1 1 0 0 1 30 24 19 5 0 5 1 0 0 1 2 0 0 0 1 0 0 0 117 114 102 12 4 8 1 0 3 1 2 0 3 1 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcaaaagtatggc atgaactggctccgccaggctccagggaaggggctggagtgggtcgcaacc attcgcagtagtggtacttccata cactatgccgactccgtgaagggccgattcactatcaccagagacaacgccaacaactcactgtatctgcaattgaacagcctgggagtcgaggactcggctgtgtatttctgt gcgagaggggccggcgcagaaggtaaaggtggtatggacgtc ARGAGAEGKGGMDV IGHV3-21 IGHD2-15 IGHJ3 ggattcaccttcaaaagtatggc atgaactggctccgccaggctccagggaaggggctggagtgggtcgcaacc attcgcagtagtggtacttccata cactatgccgactccgtgaagggccgattcactatcaccagagacaacgccaacaactcactgtatctgcaattgaacagcctgggagtcgaggactcggctgtgtatttctgt gcgagaggggccggcgcagaaggtaaaggtggtatggacgtc IGA1 23 212 9 14 4 7 5 16 4 0 11 8 JY8QFUQ01BV8FF 21810 productive Homsap IGHV3-13*01 F 885 90.65 194/214 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-12*01 F 3 8 7 10 8.7.10 [1.17.38.11] CATISDYRFEFW in-frame - ctctggaatcaccttcagtgactccgacatgcactgggtccgccaagctccaggagaaggtctggagtgggtcgcagctattggaactgctggtgatacatactatgcagactccgtgaagggccgattctccattaccagagagaatgccaagaactccttgtttcttcaaatgaacagcctgagagccgacgacacggctatttattactgtgcgaccattagtgactaccgatttgaattctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [275] t82>a,F28>I(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>c,Y37>S(+ - -)|a136>c,T46>P(+ + -)|a142>g,K48>E(+ - -)|t160>g,S54>A(- + -)|t171>a|c192>t|c202>g,P68>A(- - -)|g206>a,G69>D(- - -)|a229>t,T77>S(+ - +)|c234>t|t235>a,S79>T(+ - +)|a243>g|a263>t,Y88>F(- + -)|g290>a,G97>D(- - -)|g291>c,G97>D(- - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| t82>a,F28>I(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>c,Y37>S(+ - -)| a136>c,T46>P(+ + -)|a142>g,K48>E(+ - -)|t160>g,S54>A(- + -)| t171>a|c192>t| c202>g,P68>A(- - -)|g206>a,G69>D(- - -)|a229>t,T77>S(+ - +)|c234>t|t235>a,S79>T(+ - +)|a243>g|a263>t,Y88>F(- + -)|g290>a,G97>D(- - -)|g291>c,G97>D(- - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| a315>g|g317>c,R106>T(- - -)|a318>c,R106>T(- - -)|g319>a|a320>t| 314 (320) 216 (222) 196 (197) 20 (25) 4 (7) 16 (18) 3 (4) 4 (5) 2 0 2 (3) 0 2 (3) 3 1 (2) 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 0 0 1 0 0 1 0 0 0 0 51 51 48 3 0 3 1 0 0 0 1 0 0 0 0 0 0 1 30 21 19 2 2 0 0 0 1 0 0 0 0 1 0 0 0 0 117 114 103 11 2 9 1 3 1 0 0 0 2 1 1 1 1 0 2 (8) 2 2 (3) 0 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 (1) 0 0 (1) 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|ta,135-136(FR2)|aa,141-142(FR2)|aa,143-144(FR2)|ta,165-166(FR2-CDR2)|ta,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,242-243(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,135-136(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,259-260(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|agct,162-165(FR2)|ggta,169-172(CDR2)|ggct,205-208(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,162-165(FR2)|tact,171-174(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggaatcaccttcagtgactccgac atgcactgggtccgccaagctccaggagaaggtctggagtgggtcgcagct attggaactgctggtgataca tactatgcagactccgtgaagggccgattctccattaccagagagaatgccaagaactccttgtttcttcaaatgaacagcctgagagccgacgacacggctatttattactgt gcgaccattagtgactaccgatttgaattc ATISDYRFEF IGHV3-13 IGHD5-12 IGHJ4 ggaatcaccttcagtgactccgac atgcactgggtccgccaagctccaggagaaggtctggagtgggtcgcagct attggaactgctggtgataca tactatgcagactccgtgaagggccgattctccattaccagagagaatgccaagaactccttgtttcttcaaatgaacagcctgagagccgacgacacggctatttattactgt gcgaccattagtgactaccgatttgaattc IGA2 20 210 9 11 6 9 3 11 2 2 12 4 JY8QFUQ01BVBOA 5034 productive Homsap IGHV3-66*02 F 813 86.92 186/214 nt NA Homsap IGHJ3*02 F 196 88 44/50 nt Homsap IGHD3-10*01 F 1 8 7 15 8.7.15 [1.17.38.11] CARVRFGPSYLDVFDIW in-frame + ctctggattcaccgtcaatagttatcacatgagttgggttcgccaggctccagggaaggggccggagtgggtctcaattatttatccggatggtgacgcattctacgcagactccgtgaagggccgattcaccttctccagagacatttccaagaacacggtgtatctccaaattaacagagtgacaactgaggacacggctatgtactactgtgcgagagtccggttcgggccaagttatctggatgtctttgacatctggggccaagggacattggccaccgtctcttcagcatccccggaccagcccaaggtcttcccgctgagcctctgcagcaccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccga NA IGA1 79 79 [289, 290] g104>a,S35>N(- - -)|c108>t|a109>t,N37>Y(- - -)|c111>t,N37>Y(- - -)|t112>c,Y38>H(+ - -)|c120>t|c126>t|t149>c,L50>P(- - -)|g163>a,V55>I(+ - +)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>g,S58>P(+ - -)|g176>a,G59>D(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a193>g,T65>A(- - -)|a197>t,Y66>F(- + -)|a232>t,I78>F(+ - -)|a245>t,N82>I(- - -)|c259>g,L87>V(+ - +)|t267>c|g273>t,M91>I(+ + -)|c279>a,S93>R(- - -)|c280>g,L94>V(+ - +)|g284>c,R95>T(- - -)|g286>a,A96>T(- - -)|g301>a,V101>M(+ - -)|t306>c| g104>a,S35>N(- - -)|c108>t|a109>t,N37>Y(- - -)|c111>t,N37>Y(- - -)|t112>c,Y38>H(+ - -)| c120>t|c126>t|t149>c,L50>P(- - -)|g163>a,V55>I(+ - +)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>g,S58>P(+ - -)|g176>a,G59>D(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a193>g,T65>A(- - -)| a197>t,Y66>F(- + -)|a232>t,I78>F(+ - -)|a245>t,N82>I(- - -)|c259>g,L87>V(+ - +)|t267>c|g273>t,M91>I(+ + -)|c279>a,S93>R(- - -)|c280>g,L94>V(+ - +)|g284>c,R95>T(- - -)|g286>a,A96>T(- - -)|g301>a,V101>M(+ - -)|t306>c| 318 220 192 28 5 23 2 6 4 4 1 1 4 0 2 3 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 2 1 0 0 1 0 0 0 0 0 51 51 47 4 2 2 0 1 2 1 0 0 0 0 0 0 0 0 30 21 14 7 0 7 2 2 0 0 1 0 0 0 1 1 0 0 117 114 102 12 2 10 0 2 0 2 0 1 3 0 1 2 1 0 6 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccgtcaatagttatcac atgagttgggttcgccaggctccagggaaggggccggagtgggtctcaatt atttatccggatggtgacgca ttctacgcagactccgtgaagggccgattcaccttctccagagacatttccaagaacacggtgtatctccaaattaacagagtgacaactgaggacacggctatgtactactgt gcgagagtccggttcgggccaagttatctggatgtctttgacatc ARVRFGPSYLDVFDI IGHV3-66 IGHD3-10 IGHJ3 ggattcaccgtcaatagttatcac atgagttgggttcgccaggctccagggaaggggccggagtgggtctcaatt atttatccggatggtgacgca ttctacgcagactccgtgaagggccgattcaccttctccagagacatttccaagaacacggtgtatctccaaattaacagagtgacaactgaggacacggctatgtactactgt gcgagagtccggttcgggccaagttatctggatgtctttgacatc IGA1 28 210 16 12 10 17 6 11 4 1 12 11 JY8QFUQ01BVHS5 21308 productive (see comment) Homsap IGHV3-69-1*02 P 919 93.33 196/210 nt 92.38 194/210 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD1-20*01 F 3 8 7 10 8.7.10 [1.16.38.11] CARDDKWAFDYW in-frame - Potentially, because of detected insertion/deletion however the closest V is a pseudogene in FR2-IMGT at codon 41 of V-REGION: 'G' (1 nucleotides at position 35 to 35 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 55 of V-REGION: 3 nucleotides (from position 76 in the user submitted sequence), (do not cause frameshift) tctggattcaccttcagtgactacagcatgaactGgggtccgccaggctccagggcaggggctggagtgggtctcatatagtcgcggaagaaccacatactacgcagactctgtgcagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgacagccgaggacacggctgtttattactgtgcgagagatgataagtgggcatttgactactggggccagggaaccctggtcgtcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [272] t112>a,Y38>S(+ - -)|a113>g,Y38>S(+ - -)|a142>c,K48>Q(+ - -)|a166>t,I56>Y(- - -)|t167>a,I56>Y(- - -)|a172>c,S58>R(- - -)|t174>c,S58>R(- - -)|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|t189>a,S63>R(- - -)|t194>c,I65>T(- - -)|a214>c,K72>Q(+ - -)|c246>t|g284>c,R95>T(- - -)| t112>a,Y38>S(+ - -)|a113>g,Y38>S(+ - -)| a142>c,K48>Q(+ - -)| a166>t,I56>Y(- - -)|t167>a,I56>Y(- - -)|a172>c,S58>R(- - -)|t174>c,S58>R(- - -)|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|t189>a,S63>R(- - -)|t194>c,I65>T(- - -)| a214>c,K72>Q(+ - -)|c246>t|g284>c,R95>T(- - -)| 320 218 204 14 1 13 2 0 1 2 3 0 1 4 1 0 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 1 0 0 0 0 51 48 47 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 30 21 13 8 0 8 1 0 0 2 1 0 1 3 0 0 0 0 117 114 111 3 1 2 0 0 1 0 1 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcagtgactacagc atgaactgggtccgccaggctccagggcaggggctggagtgggtctca tatagtcgcggaagaaccaca tactacgcagactctgtgcagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgacagccgaggacacggctgtttattactgt gcgagagatgataagtgggcatttgactac ARDDKWAFDY IGHV3-69-1 IGHD1-20 IGHJ4 ggattcaccttcagtgactacagc atgaactgggtccgccaggctccagggcaggggctggagtgggtctca tatagtcgcggaagaaccaca tactacgcagactctgtgcagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgacagccgaggacacggctgtttattactgt gcgagagatgataagtgggcatttgactac IGA1 14 207 5 9 1 2 4 12 1 0 3 10 JY8QFUQ01BVMIC 14748 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F or Homsap IGHV5-51*04 F 938 91.86 203/221 nt 90.95 201/221 nt Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 196 88 44/50 nt Homsap IGHD5-18*01 F 1 8 8 12 8.8.12 [2.17.38.11] CARRDVGSHAFDLW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'T' (1 nucleotides at position 13 to 13 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 40 of V-REGION: 'G' (1 nucleotides at position 38 to 38 in the user submitted sequence), (cause frameshift); ggggttctggatTacaattttaactacgactggatcgGgctgggtgcgccagatgcccgggaaaggcctggagtggatggggatggtctatgttggtgactctgatgctagatacagcccgtcctccgaaggccaggtcaccatctcagccgacaaggccatcagtaccgcctacctgcagtggagtagcctgaaggcctcggacaccgccatgtattactgtgcgagacgggatgtgggttcacatgctttcgatctgtggggccaagggacaatggtcaccgtttcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [290] a71>g|g86>a,S29>N(- - -)|c87>t,S29>N(- - -)|c104>a,T35>N(- + -)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|t109>g,Y37>D(- - -)|c165>g,I55>M(+ + -)|a166>g,I56>V(+ - +)|c172>g,P58>V(- - -)|c173>t,P58>V(- - -)|a193>g,T65>A(- - -)|c195>t,T65>A(- - -)|t212>c,F71>S(- - -)|c214>g,Q72>E(+ + -)|t247>g,S83>A(- + -)|c255>t|c276>t| a71>g| g86>a,S29>N(- - -)|c87>t,S29>N(- - -)|c104>a,T35>N(- + -)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|t109>g,Y37>D(- - -)| c165>g,I55>M(+ + -)| a166>g,I56>V(+ - +)|c172>g,P58>V(- - -)|c173>t,P58>V(- - -)|a193>g,T65>A(- - -)|c195>t,T65>A(- - -)| t212>c,F71>S(- - -)|c214>g,Q72>E(+ + -)|t247>g,S83>A(- + -)|c255>t|c276>t| a320>g| 319 (320) 228 (229) 210 18 (19) 3 (4) 15 3 (4) 2 5 1 0 1 1 0 0 3 0 2 78 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 2 1 0 0 1 1 0 0 0 0 1 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 19 5 0 5 2 0 2 0 0 0 0 0 0 1 0 0 117 114 109 5 2 3 0 0 2 1 0 0 0 0 0 1 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ggggttct ggatacaattttaactacgactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatg gtctatgttggtgactctgatgct agatacagcccgtcctccgaaggccaggtcaccatctcagccgacaaggccatcagtaccgcctacctgcagtggagtagcctgaaggcctcggacaccgccatgtattactgt gcgagacgggatgtgggttcacatgctttcgatctg ARRDVGSHAFDL IGHV5-51 IGHD5-18 IGHJ3 ggatacaattttaactacgactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatg gtctatgttggtgactctgatgct agatacagcccgtcctccgaaggccaggtcaccatctcagccgacaaggccatcagtaccgcctacctgcagtggagtagcctgaaggcctcggacaccgccatgtattactgt gcgagacgggatgtgggttcacatgctttcgatctg IGA1 17 213 10 7 7 11 3 6 2 0 4 11 JY8QFUQ01BVRVR 10736 productive (see comment) Homsap IGHV3-66*01 F, or Homsap IGHV3-66*02 F or Homsap IGHV3-66*04 F 963 92.04 208/226 nt 91.59 207/226 nt Homsap IGHJ4*02 F 132 75 36/48 nt NA 8 7 9 8.7.9 [5.17.38.11] CARGGPHFYDS (see Functionality comment) in-frame - Potentially, because of detected insertion/deletion, IMGT/JunctionAnalysis gives no results for this JUNCTION in CDR1-IMGT at codon 29 of V-REGION: 'C' (1 nucleotides at position 23 to 23 in the user submitted sequence), (cause frameshift); gccgtctgcagcctctggattcCattgtcaatagcaactacatgagttgggtccgccaggctccagggaaggggctggactgcgtctcagttatttatcccggtggtagcacatactatgcagactccgtgaagggccgattcaccatctccagagacatctccaagaacacactgtatcttcaaatgaacagcctgagagccgaggacacggctgtatattactgtgcgagagggggcccccacttttatgatagtgttaagggaaccctggtcaccgtctcttcagcctccaccaaggggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 94 [287] c63>g|t64>c,S22>P(+ - -)|c66>g,S22>P(+ - -)|g68>c,C23>S(- - -)|c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g104>a,S35>N(- - -)|c120>t|g153>c,E51>D(+ - +)|g156>c,W52>C(+ - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c201>t|a223>c|a245>t,N82>I(- - -)|t246>c,N82>I(- - -)|g258>a|g303>a| c63>g|t64>c,S22>P(+ - -)|c66>g,S22>P(+ - -)|g68>c,C23>S(- - -)| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g104>a,S35>N(- - -)| c120>t|g153>c,E51>D(+ - +)|g156>c,W52>C(+ - -)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)| c201>t|a223>c|a245>t,N82>I(- - -)|t246>c,N82>I(- - -)|g258>a|g303>a| a320>g| 319 (320) 233 (234) 215 18 (19) 6 (7) 12 0 (1) 3 4 2 2 0 1 0 4 2 0 0 78 16 12 4 1 3 0 0 0 1 0 0 0 0 1 2 0 0 36 24 21 3 0 3 0 1 2 0 0 0 0 0 0 0 0 0 51 51 48 3 1 2 0 0 1 0 0 0 0 0 2 0 0 0 30 21 19 2 0 2 0 0 0 0 1 0 0 0 1 0 0 0 117 114 108 6 4 2 0 2 1 1 1 0 1 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|ggca,220-223(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gccgtctgcagcctct ggattcattgtcaatagcaactac atgagttgggtccgccaggctccagggaaggggctggactgcgtctcagtt atttatcccggtggtagcaca tactatgcagactccgtgaagggccgattcaccatctccagagacatctccaagaacacactgtatcttcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgagagggggcccccacttttatgat ARGGPHFYD IGHV3-66 IGHJ4 ggattcattgtcaatagcaactac atgagttgggtccgccaggctccagggaaggggctggactgcgtctcagtt atttatcccggtggtagcaca tactatgcagactccgtgaagggccgattcaccatctccagagacatctccaagaacacactgtatcttcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgagagggggcccccacttttatgat IGG2 14 210 8 6 7 10 1 4 5 0 4 5 JY8QFUQ01BWBZS 14237 productive (see comment) Homsap IGHV3-72*01 F 952 92.34 205/222 nt 91.89 204/222 nt Homsap IGHJ6*02 F 193 79.03 49/62 nt Homsap IGHD6-19*01 F 1 8 10 14 8.10.14 [1.16.38.11] CARLHYSGGWYGLDVW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 52 of V-REGION: 1 nucleotides (from position 70 in the user submitted sequence), (cause frameshift) ctctggattcaccttcagtgaccactacatagactgggtccgccaggctccaggaaaggggctggagtggttggccgtactcgaaataaagctaacggttacagtacagagtatgccgcgtctgtgaaaggcagattcaccgtctcaagagatgactcagagaacttagtgcatctgcaaatgaacagcctgaaaagcgaggacacggccctgtattactgtgctagactgcactacagtggtggctggtacggtctggacgtctggggccaagggaccacagtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [295] g117>a,M39>I(+ + -)|g141>a|a169>c|c174>t|a184>g,S62>G(+ + -)|c191>g,T64>S(+ - +)|c192>t,T64>S(+ - +)|a198>g|c201>t|a232>g,I78>V(+ - +)|t246>c|a250>g,K84>E(+ - -)|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)|c287>g,T96>S(+ - +)|g301>c,V101>L(+ - +)| g117>a,M39>I(+ + -)|g141>a| a169>c|c174>t|a184>g,S62>G(+ + -)|c191>g,T64>S(+ - +)|c192>t,T64>S(+ - +)| a198>g|c201>t|a232>g,I78>V(+ - +)|t246>c|a250>g,K84>E(+ - -)|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)|c287>g,T96>S(+ - +)|g301>c,V101>L(+ - +)| g319>c|a320>t| 318 (320) 228 (230) 211 17 (19) 6 (8) 11 4 2 4 2 1 0 0 (1) 0 1 (2) 3 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 50 48 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 30 30 25 5 2 3 1 0 2 0 1 0 0 0 0 1 0 0 117 114 104 10 3 7 3 0 2 2 0 0 0 0 1 2 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,165-168(FR2-CDR2)|agct,177-180(CDR2)|aact,253-256(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tgct,312-315(FR3)| ctct ggattcaccttcagtgaccactac atagactgggtccgccaggctccaggaaaggggctggagtggttggccgt actcgaaataaagctaacggttacagtaca gagtatgccgcgtctgtgaaaggcagattcaccgtctcaagagatgactcagagaacttagtgcatctgcaaatgaacagcctgaaaagcgaggacacggccctgtattactgt gctagactgcactacagtggtggctggtacggtctggacgtc ARLHYSGGWYGLDV IGHV3-72 IGHD6-19 IGHJ6 ggattcaccttcagtgaccactac atagactgggtccgccaggctccaggaaaggggctggagtggttggccgt actcgaaataaagctaacggttacagtaca gagtatgccgcgtctgtgaaaggcagattcaccgtctcaagagatgactcagagaacttagtgcatctgcaaatgaacagcctgaaaagcgaggacacggccctgtattactgt gctagactgcactacagtggtggctggtacggtctggacgtc IGA1 17 218 12 5 6 10 6 7 4 2 8 3 JY8QFUQ01BWD62 17595 productive (see comment) Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F 872 89.77 193/215 nt 88.84 191/215 nt Homsap IGHJ3*02 F 187 86 43/50 nt Homsap IGHD6-13*01 F 1 8 7 14 8.7.14 [1.17.38.11] CARPTYSSGRDAFDTW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 55 of V-REGION: 'GA' (2 nucleotides at position 79 to 80 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 64 of V-REGION: 'C' (1 nucleotides at position 99 to 99 in the user submitted sequence), (cause frameshift); tatatggtgggtccttcagtacttactactggacatggatccgccagcacccagagaagggactggagtggattggggGAaaatcaatcacagtggaaCgccccaactacagcccgtccctcaatagtcgagtcatcatatcgatagacacgtccaagaaccaggtctccctgaagctcttctctgtgaccgccgcggacacgggtgtgtactattgtgcgaggcccacgtatagtagcggccgggacgcctttgatacctggggccaagggacgacggtcaccgtctcatcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccga NA IGA1 100 100 [291] c75>a|g106>a,G36>T(+ - -)|g107>c,G36>T(+ - -)|g119>c,S40>T(+ - +)|c120>a,S40>T(+ - +)|c134>a,P45>H(+ - -)|g140>a,G47>E(- - -)|g147>a|t174>c|a193>c,T65>P(+ + -)|a203>g,N68>S(- - -)|g216>t,K72>N(+ - -)|c230>t,T77>I(- - -)|a237>g|g238>a,V80>I(+ - +)|t259>g,F87>V(+ - -)|g273>c|a274>t,S92>F(- - -)|g275>t,S92>F(- - -)|c299>g,A100>G(- + -)|t306>c|c309>t| c75>a| g106>a,G36>T(+ - -)|g107>c,G36>T(+ - -)| g119>c,S40>T(+ - +)|c120>a,S40>T(+ - +)|c134>a,P45>H(+ - -)|g140>a,G47>E(- - -)|g147>a| t174>c|a193>c,T65>P(+ + -)| a203>g,N68>S(- - -)|g216>t,K72>N(+ - -)|c230>t,T77>I(- - -)|a237>g|g238>a,V80>I(+ - +)|t259>g,F87>V(+ - -)|g273>c|a274>t,S92>F(- - -)|g275>t,S92>F(- - -)|c299>g,A100>G(- + -)|t306>c|c309>t| a318>g|g319>c|g320>c| 317 (320) 220 (223) 198 22 (25) 7 (10) 15 2 (3) 4 2 2 1 3 1 0 3 (5) 1 2 1 78 5 4 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 51 51 46 5 1 4 0 2 0 0 0 2 0 0 1 0 0 0 30 21 19 2 1 1 0 0 0 1 1 0 0 0 0 0 0 0 117 114 102 12 4 8 2 1 2 1 0 0 1 0 1 1 2 1 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 0 0 0 0 0 0 0 (2) 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tatat ggtgggtccttcagtacttactac tggacatggatccgccagcacccagagaagggactggagtggattggggaa atcaatcacagtggaagcccc aactacagcccgtccctcaatagtcgagtcatcatatcgatagacacgtccaagaaccaggtctccctgaagctcttctctgtgaccgccgcggacacgggtgtgtactattgt gcgaggcccacgtatagtagcggccgggacgcctttgatacc ARPTYSSGRDAFDT IGHV4-34 IGHD6-13 IGHJ3 ggtgggtccttcagtacttactac tggacatggatccgccagcacccagagaagggactggagtggattggggaa atcaatcacagtggaagcccc aactacagcccgtccctcaatagtcgagtcatcatatcgatagacacgtccaagaaccaggtctccctgaagctcttctctgtgaccgccgcggacacgggtgtgtactattgt gcgaggcccacgtatagtagcggccgggacgcctttgatacc IGA1 21 210 10 11 6 14 4 7 5 1 12 3 JY8QFUQ01BWIHJ 8251 productive (see comment) Homsap IGHV3-7*01 F 927 92.17 200/217 nt 91.71 199/217 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-3*02 F 2 8 8 14 8.8.14 [1.17.38.11] CARGSYWHYDTFGDYW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 65 of V-REGION: 'C' (1 nucleotides at position 103 to 103 in the user submitted sequence), (cause frameshift); ctctggatttacttttaacaactattggatgacctgggttcgccaggctccagggaaggggctggaatgggtggccaacataaaacaacatggaggtgaaaaCgtactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgtgcgagagggtcgtattggcattatgataccttcggcgactattggggcccgggagtcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [292] c84>t|c87>t|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|c126>t|g153>a|g171>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a195>g|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| c84>t|c87>t|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|c126>t|g153>a| g171>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a195>g| a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| a320>g| 319 (320) 224 (225) 207 17 (18) 8 (9) 9 3 (4) 5 3 1 1 0 0 0 3 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 2 2 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 0 1 0 0 0 30 24 19 5 3 2 2 2 0 0 0 0 0 0 1 0 0 0 117 114 110 4 1 3 1 0 0 0 1 0 0 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatttacttttaacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccaac ataaaacaacatggaggtgaaaag tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat ARGSYWHYDTFGDY IGHV3-7 IGHD3-3 IGHJ4 ggatttacttttaacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccaac ataaaacaacatggaggtgaaaag tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat IGG1 17 213 12 5 8 12 4 5 3 5 4 5 JY8QFUQ01BX0QN 4528 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD6-6*01 F 1 8 8 16 8.8.16 [1.17.38.11] CAREGGRGSSSEDYFDYW in-frame + ctctggattcaccttcagtagctatactatgaactgggtccgccaggctccagggaaggggctggagtacgtctcatccattagtagcaatggtgcttacatatactacgcagactcaatggagggccgattcaccatctccagagacaacgccaggaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtctattactgtgcgcgagagggggggcggggcagttcgtcggaagactactttgactactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [296, 295] g113>c,S38>T(+ - +)|c114>t,S38>T(+ - +)|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|t174>c|g176>a,S59>N(- - -)|a184>g,S62>G(+ + -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|g211>a,V71>M(+ - -)|a214>g,K72>E(+ - -)|a251>g,K84>R(+ + +)|c265>t|g303>c|a316>c| g113>c,S38>T(+ - +)|c114>t,S38>T(+ - +)| g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)| t174>c|g176>a,S59>N(- - -)|a184>g,S62>G(+ + -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)| g211>a,V71>M(+ - -)|a214>g,K72>E(+ - -)|a251>g,K84>R(+ + +)|c265>t|g303>c| a316>c| 320 225 210 15 4 11 4 3 2 1 1 0 0 0 4 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 1 0 0 0 0 0 1 0 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 30 24 19 5 1 4 2 1 0 1 0 0 0 0 1 0 0 0 117 114 109 5 2 3 2 1 1 0 0 0 0 0 1 0 0 0 8 8 7 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctatact atgaactgggtccgccaggctccagggaaggggctggagtacgtctcatcc attagtagcaatggtgcttacata tactacgcagactcaatggagggccgattcaccatctccagagacaacgccaggaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgcgagagggggggcggggcagttcgtcggaagactactttgactac AREGGRGSSSEDYFDY IGHV3-21 IGHD6-6 IGHJ4 ggattcaccttcagtagctatact atgaactgggtccgccaggctccagggaaggggctggagtacgtctcatcc attagtagcaatggtgcttacata tactacgcagactcaatggagggccgattcaccatctccagagacaacgccaggaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgcgagagggggggcggggcagttcgtcggaagactactttgactac IGA1 14 213 10 4 5 9 5 5 2 1 5 6 JY8QFUQ01BX1DY 9397 productive (see comment) Homsap IGHV3-30*04 F, or Homsap IGHV3-30-3*01 F or Homsap IGHV3-30-3*03 F 891 90.32 196/217 nt 89.86 195/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-19*01 F 1 8 8 10 8.8.10 [1.17.38.11] CVRGSSGRFDSW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 12 to 12 in the user submitted sequence), (cause frameshift); ctctggattcaGccttcagaaaatatgctatgcactgggtccgccaggctccaggcaaggggctggaatgggtggcgattatatcctatgatggaagaagtccatactacgcagactccgtgaggggccgattcaccatctccagagacaattccaggagtactctggatctgcagatgaacagcctgagacctgaggacacggctgtatattcctgtgtgagaggaagcagtggccgctttgactcctggggcccgggaacgctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [280] t105>a,S35>R(- - -)|g107>a,S36>K(- - -)|c108>a,S36>K(- - -)|g153>a|a162>g|g163>a,V55>I(+ - +)|a171>c|t189>a,S63>R(- - -)|a191>g,N64>S(- - -)|a193>c,K65>P(- - -)|a194>c,K65>P(- - -)|a215>g,K72>R(+ + +)|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>t|t262>g,Y88>D(- - -)|a270>g|g286>c,A96>P(- - -)|g303>a|a308>c,Y103>S(+ - -)|c314>t,A105>V(+ - +)| t105>a,S35>R(- - -)|g107>a,S36>K(- - -)|c108>a,S36>K(- - -)| g153>a|a162>g|g163>a,V55>I(+ - +)| a171>c|t189>a,S63>R(- - -)|a191>g,N64>S(- - -)|a193>c,K65>P(- - -)|a194>c,K65>P(- - -)| a215>g,K72>R(+ + +)|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>t|t262>g,Y88>D(- - -)|a270>g|g286>c,A96>P(- - -)|g303>a|a308>c,Y103>S(+ - -)| c314>t,A105>V(+ - +)|a320>g| 319 (320) 224 (225) 202 22 (23) 6 (7) 16 6 (7) 4 2 0 4 1 0 2 1 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 1 0 1 0 0 0 0 51 51 48 3 2 1 1 2 0 0 0 0 0 0 0 0 0 0 30 24 19 5 1 4 1 0 0 0 3 0 0 1 0 0 0 0 117 114 104 10 3 7 4 1 1 0 1 0 0 0 1 0 1 1 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagaaaatatgct atgcactgggtccgccaggctccaggcaaggggctggaatgggtggcgatt atatcctatgatggaagaagtcca tactacgcagactccgtgaggggccgattcaccatctccagagacaattccaggagtactctggatctgcagatgaacagcctgagacctgaggacacggctgtatattcctgt gtgagaggaagcagtggccgctttgactcc VRGSSGRFDS IGHV3-30 IGHD6-19 IGHJ4 ggattcaccttcagaaaatatgct atgcactgggtccgccaggctccaggcaaggggctggaatgggtggcgatt atatcctatgatggaagaagtcca tactacgcagactccgtgaggggccgattcaccatctccagagacaattccaggagtactctggatctgcagatgaacagcctgagacctgaggacacggctgtatattcctgt gtgagaggaagcagtggccgctttgactcc IGG1 21 213 11 10 5 8 6 13 5 1 8 7 JY8QFUQ01BX1X7 18514 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 825 86.36 190/220 nt 85 187/220 nt Homsap IGHJ6*02 F 187 79.66 47/59 nt Homsap IGHD2-21*01 F 2 8 8 11 8.8.11 [2.17.38.11] CARRDSDFRMDVW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'A' (1 nucleotides at position 25 to 25 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 61 to 61 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 91 of V-REGION: 'T' (1 nucleotides at position 181 to 181 in the user submitted sequence), (cause frameshift); ggtgtctggatatgactttagcagAattttggatcgcctgggtgcgccagatgcccgggaAaaggcctggagtggattggaatcatctatcctagtgactctgatacgagatacagtcccaccttccaaggccaggtcattatctcagccgacaagtccctcagtaccgcctacctacttTtttagcagtctgagggcctcggacaccgccatgtatttttgtgcgagacgtgattccgacttccgtatggacgtctggggccaggggaccacggtcaccgtgtctccaacatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] g74>t,G25>V(- - -)|t75>g,G25>V(- - -)|c84>t|a85>g,S29>D(- - -)|g86>a,S29>D(- - -)|c104>g,T35>S(+ - +)|c108>a,S36>R(- - -)|a110>t,Y37>F(- + -)|c111>t,Y37>F(- + -)|g119>c,G40>A(- + -)|g159>t,M53>I(+ + -)|g162>a|g175>a,G59>S(+ + -)|c195>g|c204>t|g207>c|t208>a,S70>T(+ - +)|c230>t,T77>I(- - -)|c231>t,T77>I(- - -)|a250>c,I84>L(+ + +)|c255>t|g267>a|a269>t,Q90>L(- - -)|g270>t,Q90>L(- - -)|g272>t,W91>F(+ + -)|g273>t,W91>F(+ + -)|c279>t|a284>g,K95>R(+ + +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g74>t,G25>V(- - -)|t75>g,G25>V(- - -)| c84>t|a85>g,S29>D(- - -)|g86>a,S29>D(- - -)|c104>g,T35>S(+ - +)|c108>a,S36>R(- - -)|a110>t,Y37>F(- + -)|c111>t,Y37>F(- + -)| g119>c,G40>A(- + -)|g159>t,M53>I(+ + -)|g162>a| g175>a,G59>S(+ + -)|c195>g| c204>t|g207>c|t208>a,S70>T(+ - +)|c230>t,T77>I(- - -)|c231>t,T77>I(- - -)|a250>c,I84>L(+ + +)|c255>t|g267>a|a269>t,Q90>L(- - -)|g270>t,Q90>L(- - -)|g272>t,W91>F(+ + -)|g273>t,W91>F(+ + -)|c279>t|a284>g,K95>R(+ + +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| a320>g| 319 (320) 227 (228) 197 30 (31) 8 (9) 22 2 (3) 4 8 0 1 1 3 1 2 2 5 1 78 7 5 2 0 2 0 0 0 0 0 0 0 0 0 0 1 1 36 24 17 7 1 6 1 1 2 0 0 1 1 0 0 1 0 0 51 51 48 3 1 2 0 1 0 0 0 0 0 0 1 0 1 0 30 24 22 2 1 1 0 1 0 0 0 0 0 0 0 1 0 0 117 114 98 16 5 11 1 1 6 0 1 0 2 1 1 0 3 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ggtgtct ggatatgactttagcagattttgg atcgcctgggtgcgccagatgcccgggaaaggcctggagtggattggaatc atctatcctagtgactctgatacg agatacagtcccaccttccaaggccaggtcattatctcagccgacaagtccctcagtaccgcctacctactttttagcagtctgagggcctcggacaccgccatgtatttttgt gcgagacgtgattccgacttccgtatggacgtc ARRDSDFRMDV IGHV5-51 IGHD2-21 IGHJ6 ggatatgactttagcagattttgg atcgcctgggtgcgccagatgcccgggaaaggcctggagtggattggaatc atctatcctagtgactctgatacg agatacagtcccaccttccaaggccaggtcattatctcagccgacaagtccctcagtaccgcctacctactttttagcagtctgagggcctcggacaccgccatgtatttttgt gcgagacgtgattccgacttccgtatggacgtc IGA1 28 213 14 14 12 21 2 7 6 2 13 7 JY8QFUQ01BX764 20739 productive (see comment) Homsap IGHV1-2*02 F 913 91.67 198/216 nt 90.74 196/216 nt Homsap IGHJ5*02 F (see comment) 174 82.35 42/51 nt Homsap IGHD3-3*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARDWGGKYAPLDNW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 45 of V-REGION: 'A' (1 nucleotides at position 47 to 47 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 57 to 57 in the user submitted sequence), (cause frameshift); tctggatacatcttcaccggctactatttgcactgggtgcgacaggAcccctggacAaagggcttgagtggatgggatacatcgacccttacactggtgacacaaattatgcacagaggtttcagggcagggtctccatgaccagggacacgtccatcagtacagcctacatggaactgaacaggctgatgtctgacgacacggccatgttttactgtgcgagagattggggtgggaagtacgcaccgctagacaactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccca NA IGA1 100 100 [288] c86>t,T29>I(- - -)|a115>t,M39>L(+ + -)|g164>a,W55>Y(- + -)|g165>c,W55>Y(- + -)|a169>g,N57>D(+ + -)|a175>t,N59>Y(- - -)|g185>c,S62>T(+ - +)|g191>a,G64>D(- - -)|c198>t|a209>g,K70>R(+ + +)|a229>t,T77>S(+ - +)|c255>t|g270>a|g275>a,S92>N(- - -)|g284>t,R95>M(- + -)|a285>g,R95>M(- + -)|g301>a,V101>M(+ - -)|a305>t,Y102>F(- + -)| c86>t,T29>I(- - -)| a115>t,M39>L(+ + -)|g164>a,W55>Y(- + -)|g165>c,W55>Y(- + -)| a169>g,N57>D(+ + -)|a175>t,N59>Y(- - -)|g185>c,S62>T(+ - +)|g191>a,G64>D(- - -)| c198>t|a209>g,K70>R(+ + +)|a229>t,T77>S(+ - +)|c255>t|g270>a|g275>a,S92>N(- - -)|g284>t,R95>M(- + -)|a285>g,R95>M(- + -)|g301>a,V101>M(+ - -)|a305>t,Y102>F(- + -)| 320 224 206 18 3 15 3 5 3 0 0 0 4 0 2 0 1 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 48 3 0 3 0 1 0 0 0 0 1 0 1 0 0 0 30 24 20 4 0 4 1 1 0 0 0 0 1 0 1 0 0 0 117 114 104 10 3 7 2 3 2 0 0 0 2 0 0 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| tct ggatacatcttcaccggctactat ttgcactgggtgcgacaggcccctggacaagggcttgagtggatgggatac atcgacccttacactggtgacaca aattatgcacagaggtttcagggcagggtctccatgaccagggacacgtccatcagtacagcctacatggaactgaacaggctgatgtctgacgacacggccatgttttactgt gcgagagattggggtgggaagtacgcaccgctagacaac ARDWGGKYAPLDN IGHV1-2 IGHD3-3 IGHJ5 ggatacatcttcaccggctactat ttgcactgggtgcgacaggcccctggacaagggcttgagtggatgggatac atcgacccttacactggtgacaca aattatgcacagaggtttcagggcagggtctccatgaccagggacacgtccatcagtacagcctacatggaactgaacaggctgatgtctgacgacacggccatgttttactgt gcgagagattggggtgggaagtacgcaccgctagacaac IGA1 18 213 11 7 8 11 3 7 3 0 10 5 JY8QFUQ01BXBPF 11396 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 932 92.2 201/218 nt NA Homsap IGHJ3*02 F 214 92 46/50 nt Homsap IGHD7-27*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARDQSLGMPEAFDIW in-frame + cctctggattcaccttcagtcgctatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcaactattagtagtcatagtatttacatatactatgcagactcagtggagggccgattcaccgtctccagagacaacgccgagaactcgctgtatctgcacatgaacaccctcagagccgacgacacggctatatattactgtgcgagagatcaaagtctggggatgcccgaggcttttgacatctggggccaagggacaatggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG3 93 100 [292] a106>c,S36>R(- - -)|t163>a,S55>T(+ - +)|c165>t,S55>T(+ - +)|a175>c,S59>H(+ - -)|g176>a,S59>H(+ - -)|g188>t,S63>I(- - -)|c201>t|a214>g,K72>E(+ - -)|a232>g,I78>V(+ - +)|a250>g,K84>E(+ - -)|a258>g|a270>c,Q90>H(- + -)|g278>c,S93>T(+ - +)|g282>c|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| a106>c,S36>R(- - -)| t163>a,S55>T(+ - +)|c165>t,S55>T(+ - +)| a175>c,S59>H(+ - -)|g176>a,S59>H(+ - -)|g188>t,S63>I(- - -)| c201>t|a214>g,K72>E(+ - -)|a232>g,I78>V(+ - +)|a250>g,K84>E(+ - -)|a258>g|a270>c,Q90>H(- + -)|g278>c,S93>T(+ - +)|g282>c|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 226 209 17 3 14 4 3 2 0 3 0 0 1 3 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 1 0 0 0 0 1 0 0 0 0 30 24 21 3 0 3 0 1 0 0 1 0 0 0 0 0 1 0 117 114 103 11 3 8 4 2 1 0 1 0 0 0 3 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtcgctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtagtcatagtatttacata tactatgcagactcagtggagggccgattcaccgtctccagagacaacgccgagaactcgctgtatctgcacatgaacaccctcagagccgacgacacggctatatattactgt gcgagagatcaaagtctggggatgcccgaggcttttgacatc ARDQSLGMPEAFDI IGHV3-21 IGHD7-27 IGHJ3 ggattcaccttcagtcgctatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtagtcatagtatttacata tactatgcagactcagtggagggccgattcaccgtctccagagacaacgccgagaactcgctgtatctgcacatgaacaccctcagagccgacgacacggctatatattactgt gcgagagatcaaagtctggggatgcccgaggcttttgacatc IGG3 17 213 9 8 5 9 4 8 3 0 10 4 JY8QFUQ01BXDPP 19216 productive Homsap IGHV3-48*01 F, or Homsap IGHV3-48*02 F 851 88.07 192/218 nt NA Homsap IGHJ6*02 F 229 85.48 53/62 nt Homsap IGHD2-2*01 F 3 8 8 19 8.8.19 [1.17.38.11] CAKDRTLHTPAQMSYYGMDVW in-frame - catctggattcaccttcagttcttatagtgtaaactgggtccgccaggctccagggaagggcctagagtttgtctcatacattgatagtagtggttctaccatatactacgcagactctgtgaagggccgattcaccatctctagagacaatgcccagaactcactgtttctgcaaatgaacaacctgcgagtcgacgacacggccgtatattactgtgcaaaagaccggacacttcacacacccgctcagatgtcttactatggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatctccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [306] c75>a|a106>t|g107>c|c108>t|c114>t|a115>g,M39>V(+ - -)|g117>a,M39>V(+ - -)|g147>c|g150>a|g155>t,W52>F(+ + -)|g156>t,W52>F(+ + -)|t159>c|a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a184>g,S62>G(+ + -)|a187>t|g188>c|c237>t|a250>c,K84>Q(+ - -)|a263>t,Y88>F(- + -)|g278>a,S93>N(- - -)|a283>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t300>c|g303>a|g315>a|g317>a,R106>K(+ + +)| c75>a| a106>t|g107>c|c108>t|c114>t| a115>g,M39>V(+ - -)|g117>a,M39>V(+ - -)|g147>c|g150>a|g155>t,W52>F(+ + -)|g156>t,W52>F(+ + -)|t159>c| a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a184>g,S62>G(+ + -)|a187>t|g188>c| c237>t|a250>c,K84>Q(+ - -)|a263>t,Y88>F(- + -)|g278>a,S93>N(- - -)|a283>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t300>c|g303>a| g315>a|g317>a,R106>K(+ + +)| 320 226 198 28 15 13 3 7 4 2 2 1 3 0 4 0 2 0 78 5 4 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 36 24 20 4 4 0 0 0 2 0 0 0 1 0 1 0 0 0 51 51 44 7 3 4 1 2 0 1 0 0 0 0 1 0 2 0 30 24 19 5 2 3 2 1 0 0 0 0 1 0 1 0 0 0 117 114 105 9 4 5 0 2 2 1 2 0 1 0 1 0 0 0 8 8 6 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| catct ggattcaccttcagttcttatagt gtaaactgggtccgccaggctccagggaagggcctagagtttgtctcatac attgatagtagtggttctaccata tactacgcagactctgtgaagggccgattcaccatctctagagacaatgcccagaactcactgtttctgcaaatgaacaacctgcgagtcgacgacacggccgtatattactgt gcaaaagaccggacacttcacacacccgctcagatgtcttactatggtatggacgtc AKDRTLHTPAQMSYYGMDV IGHV3-48 IGHD2-2 IGHJ6 ggattcaccttcagttcttatagt gtaaactgggtccgccaggctccagggaagggcctagagtttgtctcatac attgatagtagtggttctaccata tactacgcagactctgtgaagggccgattcaccatctctagagacaatgcccagaactcactgtttctgcaaatgaacaacctgcgagtcgacgacacggccgtatattactgt gcaaaagaccggacacttcacacacccgctcagatgtcttactatggtatggacgtc IGA1 25 213 14 11 9 15 5 10 7 6 9 3 JY8QFUQ01BXEXG 9600 productive (see comment) Homsap IGHV4-39*02 F 933 91.82 202/220 nt 91.36 201/220 nt Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD3-9*01 F 1 10 7 18 10.7.18 [1.17.38.11] CARLLRVYDWYSQRGYFDTW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 54 to 54 in the user submitted sequence), (cause frameshift); ctccgatggctccatcagcactagtaattactactggggctgggtccgccagcCccccagggaaggggctggagtggattggaagtatatattatagtgggagcaccgactacagctcgtccctcaggagtcgcgtcaccatatccagagacacgtccaagaaccacttctccctgaaggtgacctctgtgaccgccgcagacacggctatatattactgtgcgagactcttacgggtttatgactggtacagtcaaaggggttacttcgacacctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 86 94 [307] t78>c|g80>a,G27>D(- - -)|g101>c,S34>T(+ - +)|g107>a,S36>N(- - -)|a124>g,I42>V(+ - +)|g162>a|c168>a|t196>g,Y66>D(- - -)|a203>g,N68>S(- - -)|c205>t,P69>S(+ - -)|a215>g,K72>R(+ + +)|a225>c|g238>a,V80>R(- - -)|t239>g,V80>R(- - -)|c271>g,L91>V(+ - +)|g275>c,S92>T(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| t78>c| g80>a,G27>D(- - -)|g101>c,S34>T(+ - +)|g107>a,S36>N(- - -)| a124>g,I42>V(+ - +)|g162>a| c168>a| t196>g,Y66>D(- - -)|a203>g,N68>S(- - -)|c205>t,P69>S(+ - -)|a215>g,K72>R(+ + +)|a225>c|g238>a,V80>R(- - -)|t239>g,V80>R(- - -)|c271>g,L91>V(+ - +)|g275>c,S92>T(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g319>c|a320>t| 318 (320) 226 (228) 208 18 (20) 4 (6) 14 3 6 1 1 1 1 0 (1) 0 2 (3) 1 0 2 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 30 27 3 0 3 0 2 0 0 0 0 0 0 1 0 0 0 51 51 49 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 30 21 20 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 117 114 103 11 1 10 2 3 1 0 1 0 0 0 1 1 0 2 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctcc gatggctccatcagcactagtaattactac tggggctgggtccgccagcccccagggaaggggctggagtggattggaagt atatattatagtgggagcacc gactacagctcgtccctcaggagtcgcgtcaccatatccagagacacgtccaagaaccacttctccctgaaggtgacctctgtgaccgccgcagacacggctatatattactgt gcgagactcttacgggtttatgactggtacagtcaaaggggttacttcgacacc ARLLRVYDWYSQRGYFDT IGHV4-39 IGHD3-9 IGHJ4 gatggctccatcagcactagtaattactac tggggctgggtccgccagcccccagggaaggggctggagtggattggaagt atatattatagtgggagcacc gactacagctcgtccctcaggagtcgcgtcaccatatccagagacacgtccaagaaccacttctccctgaaggtgacctctgtgaccgccgcagacacggctatatattactgt gcgagactcttacgggtttatgactggtacagtcaaaggggttacttcgacacc IGG1 17 216 10 7 7 11 3 6 2 1 11 3 JY8QFUQ01BXNFF 8780 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 940 93.06 201/216 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD6-25*01 F 1 8 8 15 8.8.15 [1.17.38.11] CARHEGYNSRYYYFGYW in-frame + tctggatacagctttgccaccttctggatcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggaatgatctttcctggtgactccgataccagatacagcccgtccttccaaggccaggtcaccttctcagccgacaagtccatcaacaccgcctacctgcagttgaacagcctgacggcctcagacaccgccgtttattactgtgcgagacatgaaggatataacagccgttactactactttggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 100 100 [292] a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|g162>a|c165>g,I55>M(+ + -)|a170>t,Y57>F(- + -)|t189>c|a232>t,I78>F(+ - -)|g254>a,S85>N(- - -)|g272>t,W91>L(+ - -)|g275>a,S92>N(- - -)|a284>c,K95>T(- - -)|g291>a|a301>g,M101>V(+ - -)|g303>t,M101>V(+ - -)| a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)| g162>a|c165>g,I55>M(+ + -)| a170>t,Y57>F(- + -)|t189>c| a232>t,I78>F(+ - -)|g254>a,S85>N(- - -)|g272>t,W91>L(+ - -)|g275>a,S92>N(- - -)|a284>c,K95>T(- - -)|g291>a|a301>g,M101>V(+ - -)|g303>t,M101>V(+ - -)| 320 224 209 15 3 12 2 4 0 1 1 0 3 0 1 1 2 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 0 0 0 0 0 1 0 1 0 0 0 51 51 49 2 1 1 0 1 0 0 0 0 0 0 0 1 0 0 30 24 22 2 1 1 0 0 0 1 0 0 1 0 0 0 0 0 117 114 106 8 1 7 1 3 0 0 1 0 1 0 0 0 2 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| tct ggatacagctttgccaccttctgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggaatg atctttcctggtgactccgatacc agatacagcccgtccttccaaggccaggtcaccttctcagccgacaagtccatcaacaccgcctacctgcagttgaacagcctgacggcctcagacaccgccgtttattactgt gcgagacatgaaggatataacagccgttactactactttggctac ARHEGYNSRYYYFGY IGHV5-51 IGHD6-25 IGHJ4 ggatacagctttgccaccttctgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggaatg atctttcctggtgactccgatacc agatacagcccgtccttccaaggccaggtcaccttctcagccgacaagtccatcaacaccgcctacctgcagttgaacagcctgacggcctcagacaccgccgtttattactgt gcgagacatgaaggatataacagccgttactactactttggctac IGA2 15 213 7 8 4 8 3 7 2 1 8 4 JY8QFUQ01BXTDZ 14224 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 949 93.52 202/216 nt NA Homsap IGHJ4*02 F 141 77.08 37/48 nt Homsap IGHD6-13*01 F 3 8 7 12 8.7.12 [2.17.38.11] CARGVGQQLPPLDW in-frame - gtctctcgtgcctccatcaatatttcctactggagctggatccggcagcccccagggaggggactggagtggattggatatatctatgatagtgggagtaccaactacaacccctccctcaagagtcgagtcaccatatctatagacacgtccaagaaccagttctccctgaggctgaactctgtgaccgctgcggacacggccgtgtattactgtgcgaggggggtggggcagcaactgccccccttagactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [283] g79>c,G27>R(- - -)|g83>c,G28>A(- + -)|g104>a,S35>N(- - -)|g107>t,S36>I(- - -)|a110>c,Y37>S(+ - -)|a143>g,K48>R(+ + +)|g162>a|t172>g,Y58>D(- - -)|c174>t,Y58>D(- - -)|c192>t|a237>t|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>a,S92>N(- - -)| g79>c,G27>R(- - -)|g83>c,G28>A(- + -)|g104>a,S35>N(- - -)|g107>t,S36>I(- - -)|a110>c,Y37>S(+ - -)| a143>g,K48>R(+ + +)|g162>a| t172>g,Y58>D(- - -)|c174>t,Y58>D(- - -)|c192>t| a237>t|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>a,S92>N(- - -)| a318>g|a320>g| 317 (320) 221 (224) 207 (208) 14 (16) 3 (5) 11 2 (4) 4 2 0 1 0 1 0 2 0 1 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 1 0 0 1 0 0 0 2 0 1 0 51 51 49 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 30 21 18 3 1 2 0 0 2 0 0 0 0 0 0 0 0 1 117 114 110 4 1 3 1 2 0 0 0 0 1 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct cgtgcctccatcaatatttcctac tggagctggatccggcagcccccagggaggggactggagtggattggatat atctatgatagtgggagtacc aactacaacccctccctcaagagtcgagtcaccatatctatagacacgtccaagaaccagttctccctgaggctgaactctgtgaccgctgcggacacggccgtgtattactgt gcgaggggggtggggcagcaactgccccccttagac ARGVGQQLPPLD IGHV4-59 IGHD6-13 IGHJ4 cgtgcctccatcaatatttcctac tggagctggatccggcagcccccagggaggggactggagtggattggatat atctatgatagtgggagtacc aactacaacccctccctcaagagtcgagtcaccatatctatagacacgtccaagaaccagttctccctgaggctgaactctgtgaccgctgcggacacggccgtgtattactgt gcgaggggggtggggcagcaactgccccccttagac IGA1 14 210 8 6 6 9 2 5 2 1 4 7 JY8QFUQ01BXZRW 12150 productive Homsap IGHV3-48*02 F 828 87.1 189/217 nt NA Homsap IGHJ6*02 F 202 80.65 50/62 nt Homsap IGHD3-16*02 F 2 8 8 13 8.8.13 [1.17.38.11] CARGRYSYAHGMDVW in-frame + ctccggattcacctccatctcctatggcatgcactgggtccgccagattccaggaaaggggctggagtcggttgcatacattagtgatagtaatagcagcatatactatgcagactctgtgaagggccgattcaccatctcccgagacaaagacaagaagtcagtatatctgcaaatgagcagcctgagagacgaggacacggctatttattactgtgcgagggggcgatacagttatgctcacggaatggacgtctggggccaagggaccacggtcatcgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [288] t78>c|t89>c,F30>S(- - -)|g104>t,S35>I(- - -)|t105>c,S35>I(- - -)|a106>t|g107>c|a112>g,S38>G(+ + -)|a118>c,N40>H(- - -)|g133>a,A45>I(+ - +)|c134>t,A45>I(+ - +)|g141>a|g155>c,W52>S(- - -)|t160>g,S54>A(- + -)|a172>g,S58>D(- - -)|g173>a,S58>D(- - -)|g185>a,S62>N(- - -)|t189>c|c191>g,T64>S(+ - +)|c201>t|a238>c|t246>a,N82>K(+ - -)|c248>a,A83>D(- - -)|c255>g,N85>K(+ - -)|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a275>g,N92>S(- - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| t78>c| t89>c,F30>S(- - -)|g104>t,S35>I(- - -)|t105>c,S35>I(- - -)|a106>t|g107>c|a112>g,S38>G(+ + -)| a118>c,N40>H(- - -)|g133>a,A45>I(+ - +)|c134>t,A45>I(+ - +)|g141>a|g155>c,W52>S(- - -)|t160>g,S54>A(- + -)| a172>g,S58>D(- - -)|g173>a,S58>D(- - -)|g185>a,S62>N(- - -)|t189>c|c191>g,T64>S(+ - +)| c201>t|a238>c|t246>a,N82>K(+ - -)|c248>a,A83>D(- - -)|c255>g,N85>K(+ - -)|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a275>g,N92>S(- - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| a318>g|a320>g| 317 (320) 222 (225) 194 (195) 28 (30) 7 (9) 21 3 (5) 6 2 4 2 1 1 1 2 3 2 1 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 18 6 2 4 1 0 0 2 0 0 1 0 1 0 1 0 51 51 45 6 1 5 0 2 1 0 1 0 0 0 1 0 0 1 30 24 19 5 1 4 1 2 0 1 0 0 0 0 0 1 0 0 117 114 104 10 2 8 1 2 1 0 1 1 0 1 0 2 1 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ctcc ggattcacctccatctcctatggc atgcactgggtccgccagattccaggaaaggggctggagtcggttgcatac attagtgatagtaatagcagcata tactatgcagactctgtgaagggccgattcaccatctcccgagacaaagacaagaagtcagtatatctgcaaatgagcagcctgagagacgaggacacggctatttattactgt gcgagggggcgatacagttatgctcacggaatggacgtc ARGRYSYAHGMDV IGHV3-48 IGHD3-16 IGHJ6 ggattcacctccatctcctatggc atgcactgggtccgccagattccaggaaaggggctggagtcggttgcatac attagtgatagtaatagcagcata tactatgcagactctgtgaagggccgattcaccatctcccgagacaaagacaagaagtcagtatatctgcaaatgagcagcctgagagacgaggacacggctatttattactgt gcgagggggcgatacagttatgctcacggaatggacgtc IGG1 27 213 14 13 8 16 6 11 3 3 13 8 JY8QFUQ01BY0E2 4206 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 949 93.52 202/216 nt 92.59 200/216 nt Homsap IGHJ3*02 F 196 88 44/50 nt Homsap IGHD1-26*01 F 3 8 8 13 8.8.13 [1.17.38.11] CARFSGSYKSAFDIW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 57 to 57 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 55 of V-REGION: 'A' (1 nucleotides at position 78 to 78 in the user submitted sequence), (cause frameshift); tctggatacatctttaccgactattggatcggctgggtgcgccagacggccgggaaAaggcctggagtggatggggaAtcatctatcctggtgactctgacaccagttatggcccgtccttccaaggccaggtcaccatttcagccgaccagtccatcaccaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatatattactgtgcgagattcagtgggagttacaaatcggcttttgatatctggggccgagggacaatggtctccgtctcttcagcatcccgaccagccccaaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctgtccagggcttcttcccccgagg NA IGA1 100 84 [288] g86>t,S29>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c111>t|t134>c,M45>T(- - -)|c136>g,P46>A(- - -)|t192>c|a198>t,R66>S(- - -)|c201>t|a202>g,S68>G(+ + -)|c234>t|a244>c,K82>Q(+ - -)|g254>c,S85>T(+ - +)|g303>a,M101>I(+ + -)| g86>t,S29>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c111>t| t134>c,M45>T(- - -)|c136>g,P46>A(- - -)| t192>c| a198>t,R66>S(- - -)|c201>t|a202>g,S68>G(+ + -)|c234>t|a244>c,K82>Q(+ - -)|g254>c,S85>T(+ - +)|g303>a,M101>I(+ + -)| c319>t|a320>t| 318 (320) 222 (224) 208 14 (16) 4 (6) 10 2 2 3 (4) 2 1 0 1 (2) 0 1 1 1 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 1 0 0 0 0 0 0 0 1 0 51 51 49 2 0 2 0 0 0 1 0 0 0 0 0 1 0 0 30 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 107 7 2 5 1 1 2 0 1 0 1 0 1 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| tct ggatacatctttaccgactattgg atcggctgggtgcgccagacggccgggaaaggcctggagtggatggggatc atctatcctggtgactctgacacc agttatggcccgtccttccaaggccaggtcaccatttcagccgaccagtccatcaccaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatatattactgt gcgagattcagtgggagttacaaatcggcttttgatatc ARFSGSYKSAFDI IGHV5-51 IGHD1-26 IGHJ3 ggatacatctttaccgactattgg atcggctgggtgcgccagacggccgggaaaggcctggagtggatggggatc atctatcctggtgactctgacacc agttatggcccgtccttccaaggccaggtcaccatttcagccgaccagtccatcaccaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatatattactgt gcgagattcagtgggagttacaaatcggcttttgatatc IGA1 14 213 9 5 5 8 4 6 2 2 7 3 JY8QFUQ01BY2NW 14165 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 936 92.63 201/217 nt 92.17 200/217 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 3 8 8 12 8.8.12 [1.17.38.11] CARDTGQWKDFDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 41 of V-REGION: 'G' (1 nucleotides at position 36 to 36 in the user submitted sequence), (cause frameshift); ctctggattcatcttcagtagctactggatgcactGgggtccgccaagctccagggaaggggctggtgtgggtgtcacgtagtaatacgggggggactgacacagcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgtgcaagagatacggggcagtggaaggactttgactactggggccagggaaccctggtcaccgtctccttagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccag NA IGA2 100 100 [285] c86>t,T29>I(- - -)|c159>g|t167>g,I56>S(- - -)|g173>c,S58>T(+ - +)|t174>g,S58>T(+ - +)|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| c86>t,T29>I(- - -)| c159>g| t167>g,I56>S(- - -)|g173>c,S58>T(+ - +)|t174>g,S58>T(+ - +)|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| 320 225 209 16 3 13 3 1 1 0 1 0 0 0 3 3 1 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 30 24 16 8 0 8 2 1 0 0 0 0 0 0 2 0 0 3 117 114 108 6 2 4 1 0 0 0 1 0 0 0 1 2 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgt agtaatacgggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac ARDTGQWKDFDY IGHV3-74 IGHD6-19 IGHJ4 ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgt agtaatacgggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac IGA2 16 213 5 11 2 9 3 7 3 0 4 9 JY8QFUQ01BY2RR 10154 productive (see comment) Homsap IGHV3-9*01 F (see comment) 789 84.55 186/220 nt 84.09 185/220 nt Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 151 78 39/50 nt Homsap IGHD6-19*01 F 2 8 8 13 8.8.13 [2.17.38.11] CGRIAKPGLGALDLW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (84,55% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR1-IMGT at codon 36 of V-REGION: 'G' (1 nucleotides at position 24 to 24 in the user submitted sequence), (cause frameshift); gggcgttggattcacttttaatgGaacatggcatgcagtgggtccggcaagctccagggaagggcctggagtgggtcgcaggtatcagcggtaatggtgatgtcataggatatgcggactctgtgaagggccgagtcaccgtctccagagacaacgccaaagactctctatatttgcagatggacagtctgagagttaatgacacggccttatattattgtggccgcatagcgaagcctggtctgggcgccttagatctctggggccgtggaacaatggtcaccgtctctgaagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [291] a72>g|c74>g,A25>G(- + -)|t76>g,S26>V(- - -)|c77>t,S26>V(- - -)|c87>t|g103>a,D35>N(+ + -)|t108>a,D36>E(+ - +)|t109>c,Y37>H(+ - -)|c113>g,A38>G(- + -)|c120>g,H40>Q(- + -)|t160>g,S54>A(- + -)|t168>c|t171>c|t172>g,W58>G(- - -)|g174>t,W58>G(- - -)|a184>g,S62>G(+ + -)|g188>a,G63>D(- - -)|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|c198>a|t226>g,F76>V(+ - -)|a232>g,I78>V(+ - +)|g252>a|a253>g,N85>D(+ + -)|c258>t|g261>a|c265>t|a270>g|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g289>a,E97>N(+ - -)|g291>t,E97>N(+ - -)|g303>a|c309>t| a72>g|c74>g,A25>G(- + -)|t76>g,S26>V(- - -)|c77>t,S26>V(- - -)| c87>t|g103>a,D35>N(+ + -)|t108>a,D36>E(+ - +)|t109>c,Y37>H(+ - -)|c113>g,A38>G(- + -)| c120>g,H40>Q(- + -)|t160>g,S54>A(- + -)| t168>c|t171>c|t172>g,W58>G(- - -)|g174>t,W58>G(- - -)|a184>g,S62>G(+ + -)|g188>a,G63>D(- - -)|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)| c198>a|t226>g,F76>V(+ - -)|a232>g,I78>V(+ - +)|g252>a|a253>g,N85>D(+ + -)|c258>t|g261>a|c265>t|a270>g|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g289>a,E97>N(+ - -)|g291>t,E97>N(+ - -)|g303>a|c309>t| c314>g,A105>G(- + -)|a315>c,A105>G(- + -)|a316>c,K106>R(+ + +)|a317>g,K106>R(+ + +)|a318>c,K106>R(+ + +)|g319>a,D107>I(- - -)|a320>t,D107>I(- - -)|t321>a,D107>I(- - -)|a322>g| 313 (322) 221 (230) 187 34 (43) 12 (13) 22 (30) 7 (9) 6 (7) 6 3 0 (3) 1 0 (1) 1 (2) 0 3 (4) 3 4 78 7 3 4 1 3 1 0 1 0 0 0 0 0 0 1 0 1 36 24 19 5 1 4 0 1 1 1 0 0 0 1 0 1 0 0 51 51 49 2 0 2 0 0 0 0 0 0 0 0 0 1 0 1 30 24 16 8 2 6 2 1 0 2 0 0 0 0 0 0 2 1 117 114 99 15 8 7 4 4 4 0 0 1 0 0 0 0 1 1 1 (10) 1 1 0 (9) 0 (1) 0 (8) 0 (2) 0 (1) 0 0 0 (3) 0 0 (1) 0 (1) 0 0 (1) 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| gggcgtt ggattcacttttaatgaacatggc atgcagtgggtccggcaagctccagggaagggcctggagtgggtcgcaggt atcagcggtaatggtgatgtcata ggatatgcggactctgtgaagggccgagtcaccgtctccagagacaacgccaaagactctctatatttgcagatggacagtctgagagttaatgacacggccttatattattgt ggccgcatagcgaagcctggtctgggcgccttagatctc GRIAKPGLGALDL IGHV3-9 IGHD6-19 IGHJ3 ggattcacttttaatgaacatggc atgcagtgggtccggcaagctccagggaagggcctggagtgggtcgcaggt atcagcggtaatggtgatgtcata ggatatgcggactctgtgaagggccgagtcaccgtctccagagacaacgccaaagactctctatatttgcagatggacagtctgagagttaatgacacggccttatattattgt ggccgcatagcgaagcctggtctgggcgccttagatctc IGA2 30 213 20 10 11 17 9 13 8 3 9 10 JY8QFUQ01BY3HN 15171 productive Homsap IGHV3-30*01 F 878 89.45 195/218 nt NA Homsap IGHJ3*01 F 214 92 46/50 nt Homsap IGHD2-2*02 F 2 8 8 11 8.8.11 [1.17.38.11] CACGHMRDGYDVW in-frame + cctctggattcaccttcagtgcctttactatgcactgggtccgccaggctccaggcgagggactagagtgggtggcagctatatcatatgatggcagtaaaaaatactatgcggactttgtgaagggccgattcaccatctccagagacaatcccaagagtacactgtatctacaaatgaacggcctgggaggtgatgacacggctttgtattactgtgcgtgcggccatatgcgggatggttatgatgtctggggccaagggacaaaggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccag NA IGA2 93 100 [282, 283] a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a110>t,Y37>F(- + -)|g112>a,A38>T(- - -)|a142>g,K48>E(+ - -)|g147>a|t164>c,V55>A(+ - +)|a186>c|t192>a,N64>K(+ - -)|c201>t|a204>g|c209>t,S70>F(- - -)|c210>t,S70>F(- - -)|t247>c,S83>P(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|g267>a|a277>g,S93>G(+ + -)|a283>g,R95>G(- - -)|c287>g,A96>G(- + -)|g291>t,E97>D(+ - +)|g301>t,V101>L(+ - +)| a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a110>t,Y37>F(- + -)|g112>a,A38>T(- - -)| a142>g,K48>E(+ - -)|g147>a|t164>c,V55>A(+ - +)| a186>c|t192>a,N64>K(+ - -)| c201>t|a204>g|c209>t,S70>F(- - -)|c210>t,S70>F(- - -)|t247>c,S83>P(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|g267>a|a277>g,S93>G(+ + -)|a283>g,R95>G(- - -)|c287>g,A96>G(- + -)|g291>t,E97>D(+ - +)|g301>t,V101>L(+ - +)| a316>t,R106>C(- - -)|a318>c,R106>C(- - -)|a320>g| 315 (320) 221 (226) 198 (200) 23 (26) 6 (7) 17 (19) 6 (7) 4 4 2 1 (2) 0 1 (2) 1 1 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 0 0 0 0 1 0 1 0 0 0 51 51 48 3 1 2 1 1 0 1 0 0 0 0 0 0 0 0 30 24 22 2 1 1 0 0 0 0 1 0 0 1 0 0 0 0 117 114 100 14 4 10 4 2 4 1 0 0 0 0 0 1 2 0 3 (8) 3 3 (5) 0 (3) 0 (1) 0 (2) 0 (1) 0 0 0 0 (1) 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtgcctttact atgcactgggtccgccaggctccaggcgagggactagagtgggtggcagct atatcatatgatggcagtaaaaaa tactatgcggactttgtgaagggccgattcaccatctccagagacaatcccaagagtacactgtatctacaaatgaacggcctgggaggtgatgacacggctttgtattactgt gcgtgcggccatatgcgggatggttatgatgtc ACGHMRDGYDV IGHV3-30 IGHD2-2 IGHJ3 ggattcaccttcagtgcctttact atgcactgggtccgccaggctccaggcgagggactagagtgggtggcagct atatcatatgatggcagtaaaaaa tactatgcggactttgtgaagggccgattcaccatctccagagacaatcccaagagtacactgtatctacaaatgaacggcctgggaggtgatgacacggctttgtattactgt gcgtgcggccatatgcgggatggttatgatgtc IGA2 23 213 16 7 8 12 8 11 5 1 12 5 JY8QFUQ01BYDVO 17935 productive (see comment) Homsap IGHV3-11*01 F (see comment) 788 84.86 185/218 nt 84.4 184/218 nt Homsap IGHJ6*02 F (see comment) 220 83.87 52/62 nt Homsap IGHD1-26*01 F 3 8 8 15 8.8.15 [1.17.38.11] CARYRGSYYDYGGVDVW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (84,86% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) in FR3-IMGT at codon 81 of V-REGION: 'G' (1 nucleotides at position 147 to 147 in the user submitted sequence), (cause frameshift); cctctggcttcagattccgtgactactacatgacgtgggtccgccaggctccagggaagggtcttgagtggctttcctccatcagcagcggtagtaataccatccactactcagactcggtgaggggccgcttcaccatctccaggGgacaacaccaggaactcagtggatctgcaaatgaatagtctgagagccgaagacacggccgtctattattgtgcgaggtacagaggcagttactacgactatggcggtgtggacgtctggggccgtggcacctcggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcc NA IGA1 100 100 [295] a81>c|c86>g,T29>R(- - -)|c87>a,T29>R(- - -)|a103>c,S35>R(- - -)|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|a124>g,I42>V(+ - +)|g147>t|g150>t|g157>c,V53>L(+ - +)|a162>c|a164>c,Y55>S(+ - -)|t168>c|t171>c|t174>c|a175>g,S59>G(+ + -)|g184>a,G62>S(+ + -)|g188>a,S63>N(- - -)|a195>c|t196>c,Y66>H(+ - -)|g202>t,A68>S(- + -)|t210>g|a215>g,K72>R(+ + +)|a225>c|g247>a,A83>T(- - -)|a251>g,K84>R(+ + +)|c259>g,L87>V(+ - +)|t262>g,Y88>D(- - -)|c276>t|c279>t|g291>a|g303>c|c309>t| a81>c|c86>g,T29>R(- - -)|c87>a,T29>R(- - -)|a103>c,S35>R(- - -)| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|a124>g,I42>V(+ - +)|g147>t|g150>t|g157>c,V53>L(+ - +)|a162>c|a164>c,Y55>S(+ - -)| t168>c|t171>c|t174>c|a175>g,S59>G(+ + -)|g184>a,G62>S(+ + -)|g188>a,S63>N(- - -)|a195>c| t196>c,Y66>H(+ - -)|g202>t,A68>S(- + -)|t210>g|a215>g,K72>R(+ + +)|a225>c|g247>a,A83>T(- - -)|a251>g,K84>R(+ + +)|c259>g,L87>V(+ - +)|t262>g,Y88>D(- - -)|c276>t|c279>t|g291>a|g303>c|c309>t| a318>g|g319>t| 317 (320) 223 (226) 190 (191) 33 (35) 15 (17) 18 4 (5) 4 3 4 6 1 0 0 3 3 3 (4) 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 0 0 2 1 0 0 0 1 0 0 51 51 43 8 3 5 1 0 0 0 2 0 0 0 2 1 2 0 30 24 17 7 4 3 1 2 0 3 1 0 0 0 0 0 0 0 117 114 100 14 7 7 2 2 3 1 1 0 0 0 1 1 1 2 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggcttcagattccgtgactactac atgacgtgggtccgccaggctccagggaagggtcttgagtggctttcctcc atcagcagcggtagtaataccatc cactactcagactcggtgaggggccgcttcaccatctccagggacaacaccaggaactcagtggatctgcaaatgaatagtctgagagccgaagacacggccgtctattattgt gcgaggtacagaggcagttactacgactatggcggtgtggacgtc ARYRGSYYDYGGVDV IGHV3-11 IGHD1-26 IGHJ6 ggcttcagattccgtgactactac atgacgtgggtccgccaggctccagggaagggtcttgagtggctttcctcc atcagcagcggtagtaataccatc cactactcagactcggtgaggggccgcttcaccatctccagggacaacaccaggaactcagtggatctgcaaatgaatagtctgagagccgaagacacggccgtctattattgt gcgaggtacagaggcagttactacgactatggcggtgtggacgtc IGA1 33 213 15 18 7 17 8 16 10 5 12 6 JY8QFUQ01BYFAI 19655 productive (see comment) Homsap IGHV3-48*01 F 820 86.3 189/219 nt 85.84 188/219 nt Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD3-22*01 F 2 8 8 19 8.8.19 [2.17.38.11] CARASTFYYDGSGYRDVFDIW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 69 of V-REGION: 'G' (1 nucleotides at position 116 to 116 in the user submitted sequence), (cause frameshift); tctctggctttcagtttgagtacttataccatgaactgggtccgccaggctccagggaaggggctggagtgggtttcactcattagtaagactagtaatgtcatatactacgcggGactctgtgaagggccggttcaccatctccagagacaatgccgagaattcactgtttctgcaaatggacagcctgagtgccgaggacacgggtgtatattactgtgcgagagcttcgacgttctactacgatggcagtggttatcgtgatgtttttgatatatggggccaagggacaatggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [309] g73>t,A25>S(- + -)|c75>t,A25>S(- + -)|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)|g80>c,G27>A(- + -)|a81>t,G27>A(- + -)|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g113>c,S38>T(+ - +)|t163>c,Y55>L(- - -)|a164>t,Y55>L(- - -)|g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g176>c,S59>T(+ - +)|g188>a,S63>N(- - -)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)|a204>g|a225>g|a250>g,K84>E(+ - -)|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|a285>t,R95>S(- - -)|c299>g,A100>G(- + -)|g303>a| g73>t,A25>S(- + -)|c75>t,A25>S(- + -)|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)| g80>c,G27>A(- + -)|a81>t,G27>A(- + -)|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g113>c,S38>T(+ - +)| t163>c,Y55>L(- - -)|a164>t,Y55>L(- - -)| g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g176>c,S59>T(+ - +)|g188>a,S63>N(- - -)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)| a204>g|a225>g|a250>g,K84>E(+ - -)|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|a285>t,R95>S(- - -)|c299>g,A100>G(- + -)|g303>a| a320>c| 319 (320) 226 (227) 196 30 (31) 4 (5) 26 5 3 6 2 0 (1) 0 4 0 4 3 1 2 78 6 1 5 0 5 0 0 2 1 0 0 0 0 0 0 1 1 36 24 16 8 0 8 0 0 2 0 0 0 1 0 3 2 0 0 51 51 49 2 0 2 0 0 0 1 0 0 1 0 0 0 0 0 30 24 18 6 0 6 1 2 1 0 0 0 0 0 1 0 0 1 117 114 105 9 4 5 4 1 1 0 0 0 2 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tctctg gctttcagtttgagtacttatacc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcactc attagtaagactagtaatgtcata tactacgcggactctgtgaagggccggttcaccatctccagagacaatgccgagaattcactgtttctgcaaatggacagcctgagtgccgaggacacgggtgtatattactgt gcgagagcttcgacgttctactacgatggcagtggttatcgtgatgtttttgatata ARASTFYYDGSGYRDVFDI IGHV3-48 IGHD3-22 IGHJ3 gctttcagtttgagtacttatacc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcactc attagtaagactagtaatgtcata tactacgcggactctgtgaagggccggttcaccatctccagagacaatgccgagaattcactgtttctgcaaatggacagcctgagtgccgaggacacgggtgtatattactgt gcgagagcttcgacgttctactacgatggcagtggttatcgtgatgtttttgatata IGG1 25 213 13 12 7 14 6 11 4 0 7 14 JY8QFUQ01BYGMF 4580 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 945 93.09 202/217 nt NA Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 196 88 44/50 nt Homsap IGHD2-15*01 F 3 8 8 17 8.8.17 [1.17.38.11] CARDTLEVVSNMDDAFDLW in-frame + ctctggattccccttcagcacctatcccatgagctgggtccgccaggctccagggaaggggctggagtgggtctcatacattagtaagactactaatgacatatactatgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtatttctgtgcgagagatactctagaggtggtgtcgaatatggatgatgcttttgatttgtggggccaagggacagtggtcaatgtctcctcagcatccccgaccagcccctaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 89 [300, 299] a85>c,T29>P(+ + -)|t105>c|g107>c,S36>T(+ - +)|a112>c,S38>P(+ - -)|g113>c,S38>P(+ - -)|a119>g,N40>S(- - -)|c164>a,S55>Y(+ - -)|g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g176>c,S59>T(+ - +)|g185>c,S62>T(+ - +)|g188>a,S63>N(- - -)|t190>g,Y64>D(- - -)|c201>t|a308>t,Y103>F(- + -)| a85>c,T29>P(+ + -)|t105>c|g107>c,S36>T(+ - +)|a112>c,S38>P(+ - -)|g113>c,S38>P(+ - -)| a119>g,N40>S(- - -)|c164>a,S55>Y(+ - -)| g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g176>c,S59>T(+ - +)|g185>c,S62>T(+ - +)|g188>a,S63>N(- - -)|t190>g,Y64>D(- - -)| c201>t|a308>t,Y103>F(- + -)| 320 225 210 15 2 13 1 2 1 1 2 1 1 0 4 0 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 0 0 1 2 0 0 0 2 0 0 0 51 51 49 2 0 2 1 0 0 0 0 1 0 0 0 0 0 0 30 24 18 6 0 6 0 2 0 0 0 0 0 0 2 0 0 2 117 114 112 2 1 1 0 0 1 0 0 0 1 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattccccttcagcacctatccc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcatac attagtaagactactaatgacata tactatgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtatttctgt gcgagagatactctagaggtggtgtcgaatatggatgatgcttttgatttg ARDTLEVVSNMDDAFDL IGHV3-21 IGHD2-15 IGHJ3 ggattccccttcagcacctatccc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcatac attagtaagactactaatgacata tactatgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtatttctgt gcgagagatactctagaggtggtgtcgaatatggatgatgcttttgatttg IGA1 15 213 5 10 3 8 2 7 1 1 3 10 JY8QFUQ01BYOYM 12453 productive Homsap IGHV3-33*02 F 877 89.81 194/216 nt NA Homsap IGHJ1*01 F 197 86.54 45/52 nt Homsap IGHD6-6*01 F 3 8 8 14 8.8.14 [1.17.38.11] CARDRQDARAEHLQYW in-frame - tctggattcatcttcagtaattatggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtggctgttctgtggttcaatggaaatacgaagtattatgcagactccgtgaagggccgcttcaccatctccagagacacatccacgaacacgctgtttttgcaaatggacagcctgagagccgaggacacggctgtctattattgtgcaagagatcgtcaggacgcccgcgctgaacatctccaatactggggccagggcaccatggtcaccgtctcctcaacatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [289] c86>t,T29>I(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a162>t|a166>c,I56>L(+ + +)|a168>g,I56>L(+ + +)|a173>t,Y58>F(- + -)|t174>c,Y58>F(- + -)|g175>a,D59>N(+ + -)|g188>a,S63>N(- - -)|a191>c,N64>T(- + -)|t192>g,N64>T(- + -)|a195>g|c198>t|c212>t,A71>V(+ - +)|a225>c|a245>c,N82>T(- + -)|t246>a,N82>T(- + -)|c265>t|a274>g,N92>D(+ + -)|g303>c|c309>t|g315>a| c86>t,T29>I(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a162>t| a166>c,I56>L(+ + +)|a168>g,I56>L(+ + +)|a173>t,Y58>F(- + -)|t174>c,Y58>F(- + -)|g175>a,D59>N(+ + -)|g188>a,S63>N(- - -)|a191>c,N64>T(- + -)|t192>g,N64>T(- + -)|a195>g| c198>t|c212>t,A71>V(+ - +)|a225>c|a245>c,N82>T(- + -)|t246>a,N82>T(- + -)|c265>t|a274>g,N92>D(+ + -)|g303>c|c309>t| g315>a| 320 224 201 23 8 15 3 4 6 1 4 0 2 1 1 0 0 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 2 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 30 24 15 9 1 8 2 2 0 1 2 0 1 0 0 0 0 1 117 114 105 9 5 4 1 0 4 0 2 0 0 1 1 0 0 0 8 8 7 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 ta,5-6(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,253-254(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,5-6(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|tt,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggta,3-6(FR1)|agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcatcttcagtaattatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggctgtt ctgtggttcaatggaaatacgaag tattatgcagactccgtgaagggccgcttcaccatctccagagacacatccacgaacacgctgtttttgcaaatggacagcctgagagccgaggacacggctgtctattattgt gcaagagatcgtcaggacgcccgcgctgaacatctccaatac ARDRQDARAEHLQY IGHV3-33 IGHD6-6 IGHJ1 ggattcatcttcagtaattatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggctgtt ctgtggttcaatggaaatacgaag tattatgcagactccgtgaagggccgcttcaccatctccagagacacatccacgaacacgctgtttttgcaaatggacagcctgagagccgaggacacggctgtctattattgt gcaagagatcgtcaggacgcccgcgctgaacatctccaatac IGA1 22 213 13 9 9 10 4 12 6 1 4 11 JY8QFUQ01BYP0E 5855 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 990 95.39 207/217 nt NA Homsap IGHJ5*02 F 237 96.08 49/51 nt Homsap IGHD1-20*01 F 1 8 8 16 8.8.16 [1.17.38.11] CARDPVGISGSVNWFDPW in-frame + ctctggattcaccttcagcaaccataacatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatgtattggtagtagtagtagtgacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaagacacggctgtgtattactgtgcgagagatccggttgggataagtggaagtgtgaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [296] t105>c|g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)|g113>a,S38>N(- - -)|c164>g,S55>C(- - -)|c165>t,S55>C(- - -)|a169>g,S57>G(+ + -)|t190>g,Y64>D(- - -)|c287>t,A96>V(+ - +)|g291>a| t105>c|g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)|g113>a,S38>N(- - -)| c164>g,S55>C(- - -)|c165>t,S55>C(- - -)| a169>g,S57>G(+ + -)|t190>g,Y64>D(- - -)| c287>t,A96>V(+ - +)|g291>a| 320 225 215 10 2 8 1 3 2 2 0 0 0 0 0 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 2 0 2 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 1 0 0 0 0 0 0 1 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 0 1 117 114 112 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagcaaccataac atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatgt attggtagtagtagtagtgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaagacacggctgtgtattactgt gcgagagatccggttgggataagtggaagtgtgaactggttcgacccc ARDPVGISGSVNWFDP IGHV3-21 IGHD1-20 IGHJ5 ggattcaccttcagcaaccataac atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatgt attggtagtagtagtagtgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaagacacggctgtgtattactgt gcgagagatccggttgggataagtggaagtgtgaactggttcgacccc IGA1 10 213 8 2 5 6 3 4 1 1 3 5 JY8QFUQ01BYRXT 6756 productive Homsap IGHV3-21*04 F (see comment) 662 78.44 171/218 nt NA Homsap IGHJ4*02 F 105 68.75 33/48 nt Homsap IGHD5-12*01 F 3 8 8 13 8.8.13 [1.17.38.11] CVRLFTSDGVRGDYW in-frame - Low V-REGION identity (78,44% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release cctccggtttcatcttcagtcacttcagcatgaactgggtccgccaggctccaggaaagggcctggagtggatcgccgacatcagtagttcaagtgcatacatcacctatgcagattcagtcaggggccgattcgtcgtctccagggacgacgccaaggactccctgtacttgcaaatggacaacctgggagtcgacgatacggccacctattattgtgtgaggctcttcaccagcgatggagtgagaggcgactactggggccagggcgcccgggttctcgtctccgcagcttccaccaagggcccatcggtcttccccctggcgccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 79 100 [289] t78>c|a81>t|c86>t,T29>I(- - -)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a110>t,Y37>F(- + -)|t111>c,Y37>F(- + -)|g141>a|g147>c|g157>a,V53>I(+ - +)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)|t163>g,S55>D(- - -)|c164>a,S55>D(- - -)|t168>c|a175>t|g176>c|t177>a|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>a,S63>A(- + -)|a195>c|t196>a,Y66>T(+ - -)|a197>c,Y66>T(+ - -)|c201>t|c207>t|g213>c|a215>g,K72>R(+ + +)|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|a232>g,I78>V(+ - +)|a240>g|a244>g,N82>D(+ + -)|a253>g,N85>D(+ + -)|a258>c|t264>c|c265>t|a274>g,N92>D(+ + -)|g278>a,S93>N(- - -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c294>t|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>c,V101>T(- - -)|c309>t|c314>t,A105>V(+ - +)| t78>c| a81>t|c86>t,T29>I(- - -)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a110>t,Y37>F(- + -)|t111>c,Y37>F(- + -)| g141>a|g147>c|g157>a,V53>I(+ - +)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)|t163>g,S55>D(- - -)|c164>a,S55>D(- - -)| t168>c|a175>t|g176>c|t177>a|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>a,S63>A(- + -)|a195>c| t196>a,Y66>T(+ - -)|a197>c,Y66>T(+ - -)|c201>t|c207>t|g213>c|a215>g,K72>R(+ + +)|a229>g,T77>V(- - -)|c230>t,T77>V(- - -)|a232>g,I78>V(+ - +)|a240>g|a244>g,N82>D(+ + -)|a253>g,N85>D(+ + -)|a258>c|t264>c|c265>t|a274>g,N92>D(+ + -)|g278>a,S93>N(- - -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|c294>t|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>c,V101>T(- - -)|c309>t| c314>t,A105>V(+ - +)|a318>g|g319>c|a320>t| 317 (320) 223 (226) 175 48 (51) 18 (21) 30 9 (10) 5 9 5 5 1 3 (4) 3 6 (7) 0 0 2 78 5 4 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 18 6 1 5 0 1 1 1 1 0 2 0 0 0 0 0 51 51 44 7 2 5 0 2 0 0 1 1 0 0 1 0 0 2 30 24 16 8 5 3 1 0 0 1 1 0 1 2 2 0 0 0 117 114 89 25 9 16 8 2 7 2 2 0 0 1 3 0 0 0 5 (8) 5 4 1 (4) 0 (3) 1 0 (1) 0 1 0 0 0 0 (1) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctcc ggtttcatcttcagtcacttcagc atgaactgggtccgccaggctccaggaaagggcctggagtggatcgccgac atcagtagttcaagtgcatacatc acctatgcagattcagtcaggggccgattcgtcgtctccagggacgacgccaaggactccctgtacttgcaaatggacaacctgggagtcgacgatacggccacctattattgt gtgaggctcttcaccagcgatggagtgagaggcgactac VRLFTSDGVRGDY IGHV3-21 IGHD5-12 IGHJ4 ggtttcatcttcagtcacttcagc atgaactgggtccgccaggctccaggaaagggcctggagtggatcgccgac atcagtagttcaagtgcatacatc acctatgcagattcagtcaggggccgattcgtcgtctccagggacgacgccaaggactccctgtacttgcaaatggacaacctgggagtcgacgatacggccacctattattgt gtgaggctcttcaccagcgatggagtgagaggcgactac IGG1 46 213 26 20 13 20 13 26 11 6 21 8 JY8QFUQ01BYXJU 2407 productive Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 936 92.63 201/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVRAGFGELSGFDCW in-frame + ctctggattcacctttggttattatggcatgagctgggtccgccaagctccgggggaggggcgtgagtgggtctctggtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [288] a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)|a138>g|a142>g,K48>E(+ - -)|t149>g,L50>R(- + -)|g150>t,L50>R(- + -)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| a104>g,D35>G(- - -)|g106>t,D36>Y(- - -)| a138>g|a142>g,K48>E(+ - -)|t149>g,L50>R(- + -)|g150>t,L50>R(- + -)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 224 (225) 207 17 (18) 3 (4) 14 3 3 1 1 1 (2) 0 2 0 2 1 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 51 47 4 1 3 2 0 0 0 0 0 0 0 0 0 1 1 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ctct ggattcacctttggttattatggc atgagctgggtccgccaagctccgggggaggggcgtgagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggattcacctttggttattatggc atgagctgggtccgccaagctccgggggaggggcgtgagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 16 213 7 9 3 8 4 8 3 0 9 4 JY8QFUQ01BZEWU 11573 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F (see comment) 770 83.94 183/218 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-16*02 F 2 8 8 9 8.8.9 [1.17.38.11] CTGLTYPDDYW in-frame - Low V-REGION identity (83,94% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release gtctggattttaccttcagtaagttctggatgcattgggtccgccaagctccagggaaggggctgacttgggtctcacgtattaatcctgatgggactatcacgaactacacggactccgtgaggggccgattcatcacttccagagacaacgccaagaacacagtatatctgcagatgaacagtctgcgagtcgaggacacaggtgtatattactgtacaggactgacttatccagacgactactggggccagggatgtttggccaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [276] c74>g|c75>t|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)|g80>a,G27>D(- - -)|a81>t,G27>D(- - -)|c84>t|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a110>t,Y37>F(- + -)|c120>t|g151>a,V51>T(- - -)|t152>c,V51>T(- - -)|g153>t,V51>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g188>c,S63>T(+ - +)|g191>t,S64>I(- - -)|a195>g|g197>a,S66>N(- - -)|g202>a,A68>T(- - -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|t233>c,I78>T(- - -)|c234>t,I78>T(- - -)|g258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a270>g|a283>c|c287>t,A96>V(+ - +)|g297>a|c299>g,A100>G(- + -)|g303>a|g313>a,A105>T(- - -)|a316>g,R106>G(- - -)| c74>g|c75>t|t76>c,S26>L(- - -)|c77>t,S26>L(- - -)|t78>g,S26>L(- - -)| g80>a,G27>D(- - -)|a81>t,G27>D(- - -)|c84>t|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a110>t,Y37>F(- + -)| c120>t|g151>a,V51>T(- - -)|t152>c,V51>T(- - -)|g153>t,V51>T(- - -)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g188>c,S63>T(+ - +)|g191>t,S64>I(- - -)|a195>g| g197>a,S66>N(- - -)|g202>a,A68>T(- - -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|t233>c,I78>T(- - -)|c234>t,I78>T(- - -)|g258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a270>g|a283>c|c287>t,A96>V(+ - +)|g297>a|c299>g,A100>G(- + -)|g303>a| g313>a,A105>T(- - -)|a316>g,R106>G(- - -)|g319>c|a320>t| 318 (320) 224 (226) 187 37 (39) 10 (12) 27 4 10 7 3 2 0 2 (3) 0 2 (3) 4 2 1 78 5 0 5 2 3 0 0 2 1 0 0 0 0 0 1 0 1 36 24 18 6 1 5 0 2 1 0 0 0 2 0 0 1 0 0 51 51 47 4 1 3 0 1 1 1 0 0 0 0 0 0 1 0 30 24 19 5 1 4 1 0 0 0 1 0 0 0 2 0 1 0 117 114 99 15 5 10 2 6 3 1 1 0 0 0 0 2 0 0 6 (8) 6 4 2 (4) 0 (2) 2 1 1 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtctg gattttaccttcagtaagttctgg atgcattgggtccgccaagctccagggaaggggctgacttgggtctcacgt attaatcctgatgggactatcacg aactacacggactccgtgaggggccgattcatcacttccagagacaacgccaagaacacagtatatctgcagatgaacagtctgcgagtcgaggacacaggtgtatattactgt acaggactgacttatccagacgactac TGLTYPDDY IGHV3-74 IGHD3-16 IGHJ4 gattttaccttcagtaagttctgg atgcattgggtccgccaagctccagggaaggggctgacttgggtctcacgt attaatcctgatgggactatcacg aactacacggactccgtgaggggccgattcatcacttccagagacaacgccaagaacacagtatatctgcagatgaacagtctgcgagtcgaggacacaggtgtatattactgt acaggactgacttatccagacgactac IGA1 30 213 19 11 14 21 5 9 6 2 13 9 JY8QFUQ01C0J5I 22100 productive Homsap IGHV3-48*02 F, or Homsap IGHV3-48*04 F 949 93.52 202/216 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-18*01 F 3 8 8 13 8.8.13 [1.17.38.11] CARDRRGYSYEMDYW in-frame - tctggattcaccttcagtgtccatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcataccttagtagtggtagtgataccatatactacgcagactctgtgaggggccggttcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagtggcctgagagacgaggacacggctgtttattactgtgcgagagatcggcgtgggtatagttatgaaatggactactggggacagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcag NA IGG3 93 100 [288, 287] a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|t109>c,Y37>H(+ - -)|a166>c,I56>L(+ + +)|a175>g,S59>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a215>g,K72>R(+ + +)|a225>g|t246>c|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|a277>g,S93>G(+ + -)|g303>t| a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|t109>c,Y37>H(+ - -)| a166>c,I56>L(+ + +)|a175>g,S59>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)| a215>g,K72>R(+ + +)|a225>g|t246>c|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|a277>g,S93>G(+ + -)|g303>t| 320 224 210 14 3 11 7 1 1 2 1 0 0 0 0 0 2 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 0 0 1 0 0 0 0 0 0 1 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 2 1 0 0 1 0 0 0 0 0 0 0 117 114 107 7 3 4 4 0 1 1 0 0 0 0 0 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| tct ggattcaccttcagtgtccatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac cttagtagtggtagtgataccata tactacgcagactctgtgaggggccggttcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagtggcctgagagacgaggacacggctgtttattactgt gcgagagatcggcgtgggtatagttatgaaatggactac ARDRRGYSYEMDY IGHV3-48 IGHD5-18 IGHJ4 ggattcaccttcagtgtccatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac cttagtagtggtagtgataccata tactacgcagactctgtgaggggccggttcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagtggcctgagagacgaggacacggctgtttattactgt gcgagagatcggcgtgggtatagttatgaaatggactac IGG3 14 213 11 3 2 4 9 10 3 0 4 7 JY8QFUQ01C0LHV 11192 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 900 90.78 197/217 nt 90.32 196/217 nt Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD3-16*02 F 1 8 8 16 8.8.16 [1.17.38.11] CAKDPQQLLEDINWFDPW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 86 of V-REGION: 'A' (1 nucleotides at position 163 to 163 in the user submitted sequence), (cause frameshift); ctctggattcacctttcgcagctatgccatgagctgggtccgccaggctccaggaagggggctagagtgggtctcatctatcagtggtagtggtgataaaacaaagtacgcagactccgtgaagggccggttcaccatctccagagacaacgccaggaacacAtttttatctgcaaatggacagcctcagagccgaggacacggccgtctattactgtgcgaaagatcctcagcagttattggaagacataaactggttcgacccctggggccagggcacccaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] a103>c,S35>R(- - -)|g141>a|a143>g,K48>R(+ + +)|g150>a|g163>t,A55>S(- + -)|t168>c|g188>a,G63>D(- - -)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|t246>c|t247>g,S83>A(- + -)|a251>g,K84>R(+ + +)|g258>t|c259>t,L87>F(+ - -)|g261>t,L87>F(+ - -)|a274>g,N92>D(+ + -)|g282>c|a303>c| a103>c,S35>R(- - -)| g141>a|a143>g,K48>R(+ + +)|g150>a|g163>t,A55>S(- + -)| t168>c|g188>a,G63>D(- - -)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)| t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|t246>c|t247>g,S83>A(- + -)|a251>g,K84>R(+ + +)|g258>t|c259>t,L87>F(+ - -)|g261>t,L87>F(+ - -)|a274>g,N92>D(+ + -)|g282>c|a303>c| 320 225 205 20 7 13 3 4 1 2 2 1 0 1 1 1 3 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 51 51 47 4 2 2 1 2 0 0 0 0 0 0 0 0 1 0 30 24 20 4 1 3 0 2 0 1 0 1 0 0 0 0 0 0 117 114 103 11 4 7 2 0 1 1 1 0 0 1 1 1 2 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttcgcagctatgcc atgagctgggtccgccaggctccaggaagggggctagagtgggtctcatct atcagtggtagtggtgataaaaca aagtacgcagactccgtgaagggccggttcaccatctccagagacaacgccaggaacactttttatctgcaaatggacagcctcagagccgaggacacggccgtctattactgt gcgaaagatcctcagcagttattggaagacataaactggttcgacccc AKDPQQLLEDINWFDP IGHV3-23 IGHD3-16 IGHJ5 ggattcacctttcgcagctatgcc atgagctgggtccgccaggctccaggaagggggctagagtgggtctcatct atcagtggtagtggtgataaaaca aagtacgcagactccgtgaagggccggttcaccatctccagagacaacgccaggaacactttttatctgcaaatggacagcctcagagccgaggacacggccgtctattactgt gcgaaagatcctcagcagttattggaagacataaactggttcgacccc IGA1 20 213 10 10 5 11 5 9 6 1 9 4 JY8QFUQ01C0T0E 20992 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 923 91.74 200/218 nt NA Homsap IGHJ4*02 F (see comment) 159 81.25 39/48 nt Homsap IGHD1-26*01 F 1 8 8 15 8.8.15 [1.17.38.11] CGKPIVGATGREMPVSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) cctctggattcacctttagcaactatgccatgaactgggtccgccaggttccaggggaggggctggagtgggtctcagccattagtggcagtggtggtagcacattctacacagacgccttgcagggccgattcaccatctccagagacaattccaagaacacgttatatttgcaaatgaaaagcctgagagccggggacacggccgtgtattactgtgggaagccaatagtgggcgcaacaggtagagagatgcctgtttcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 93 100 [294, 295] g107>a,S36>N(- - -)|g119>a,S40>N(- - -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|t165>c|t174>c|a197>t,Y66>F(- + -)|g202>a,A68>T(- - -)|t208>g,S70>A(- + -)|g211>t,V71>L(+ - +)|a214>c,K72>Q(+ - -)|g225>a|c259>t|g261>a|c265>t|c276>a,N92>K(+ - -)|a290>g,E97>G(- - -)|a303>g|c314>g,A105>G(- + -)| g107>a,S36>N(- - -)| g119>a,S40>N(- - -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|t165>c| t174>c| a197>t,Y66>F(- + -)|g202>a,A68>T(- - -)|t208>g,S70>A(- + -)|g211>t,V71>L(+ - +)|a214>c,K72>Q(+ - -)|g225>a|c259>t|g261>a|c265>t|c276>a,N92>K(+ - -)|a290>g,E97>G(- - -)|a303>g| c314>g,A105>G(- + -)|a318>g|g319>c|a320>c| 317 (320) 223 (226) 204 19 (22) 7 (10) 12 3 (4) 5 3 2 1 (2) 1 1 0 0 (1) 1 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 47 4 1 3 1 1 1 1 0 0 0 0 0 0 0 0 30 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 102 12 5 7 2 3 2 0 1 1 1 0 0 0 1 1 5 (8) 5 4 1 (4) 0 (3) 1 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 1 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagcaactatgcc atgaactgggtccgccaggttccaggggaggggctggagtgggtctcagcc attagtggcagtggtggtagcaca ttctacacagacgccttgcagggccgattcaccatctccagagacaattccaagaacacgttatatttgcaaatgaaaagcctgagagccggggacacggccgtgtattactgt gggaagccaatagtgggcgcaacaggtagagagatgcctgtttcc GKPIVGATGREMPVS IGHV3-23 IGHD1-26 IGHJ4 ggattcacctttagcaactatgcc atgaactgggtccgccaggttccaggggaggggctggagtgggtctcagcc attagtggcagtggtggtagcaca ttctacacagacgccttgcagggccgattcaccatctccagagacaattccaagaacacgttatatttgcaaatgaaaagcctgagagccggggacacggccgtgtattactgt gggaagccaatagtgggcgcaacaggtagagagatgcctgtttcc IGA2 18 213 13 5 8 10 5 8 6 1 10 1 JY8QFUQ01C0V8V 13349 productive (see comment) Homsap IGHV3-7*01 F, or Homsap IGHV3-7*02 F 928 91.78 201/219 nt 91.32 200/219 nt Homsap IGHJ6*02 F 175 75.81 47/62 nt Homsap IGHD3-16*01 F 1 8 8 14 8.8.14 [2.16.38.11] CARAVGQRGYDGTDLW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 61 in the user submitted sequence), (cause frameshift) agcctctggattcatgttcagcagttattggatgagctgggtccgccaggatccagggaagggctggagtgggtggccaatataaacgaagaaggaagtgagaaatattatgtggactctgggaagggccgattcaggatctccagagacaacgccaagaattccgtgtatctgcaaatggacagcctgagagccgaggacacggctgtgtattactgtgcgagagcagtggggcagcgaggctacgacggtacagacctctggggccaagggaccacggtcatcgtctccacagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [293] c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|t90>c|t105>c|c108>t|c134>a,A45>D(- - -)|c165>t|g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|t177>a,D59>E(+ - +)|c198>t|t212>g,V71>G(- - -)|c230>g,T77>R(- - -)|c231>g,T77>R(- - -)|c255>t|a258>c|c259>g,L87>V(+ - +)|a274>g,N92>D(+ + -)| c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|t90>c|t105>c|c108>t| c134>a,A45>D(- - -)|c165>t| g171>c,K57>N(+ - -)|c172>g,Q58>E(+ + -)|t177>a,D59>E(+ - +)| c198>t|t212>g,V71>G(- - -)|c230>g,T77>R(- - -)|c231>g,T77>R(- - -)|c255>t|a258>c|c259>g,L87>V(+ - +)|a274>g,N92>D(+ + -)| a320>c| 319 (320) 226 (227) 208 18 (19) 7 (8) 11 1 0 5 2 1 (2) 1 0 1 1 5 0 1 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 3 2 0 0 2 2 0 0 0 0 0 1 0 0 51 50 48 2 1 1 0 0 1 0 0 1 0 0 0 0 0 0 30 24 21 3 0 3 0 0 0 0 0 0 0 1 1 1 0 0 117 114 106 8 3 5 1 0 2 0 1 0 0 0 0 3 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct ggattcatgttcagcagttattgg atgagctgggtccgccaggatccagggaagggctggagtgggtggccaat ataaacgaagaaggaagtgagaaa tattatgtggactctgggaagggccgattcaggatctccagagacaacgccaagaattccgtgtatctgcaaatggacagcctgagagccgaggacacggctgtgtattactgt gcgagagcagtggggcagcgaggctacgacggtacagacctc ARAVGQRGYDGTDL IGHV3-7 IGHD3-16 IGHJ6 ggattcatgttcagcagttattgg atgagctgggtccgccaggatccagggaagggctggagtgggtggccaat ataaacgaagaaggaagtgagaaa tattatgtggactctgggaagggccgattcaggatctccagagacaacgccaagaattccgtgtatctgcaaatggacagcctgagagccgaggacacggctgtgtattactgt gcgagagcagtggggcagcgaggctacgacggtacagacctc IGG1 18 212 8 10 5 12 3 6 4 3 6 5 JY8QFUQ01C0YWV 4423 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 922 90.22 203/225 nt NA Homsap IGHJ6*02 F 238 87.1 54/62 nt Homsap IGHD4-11*01 ORF 2 8 10 22 8.10.22 [2.17.38.11] CATDRPYYKNAAGRLWYYYGMDVW in-frame - tgctctggattcactttcactaacgcgtggatgagttgggtccgccaggctccagggaaggggctggagtgggttgcccgtgttaaaactaagactgacgatggggcaacagactacgctgcacccgtgaaaggcagattccttatctcaagagatgattcaaacaacatactgtatctgcaaatgaacagcctgagaaccgaggacacagccatgtactactgtgccacagatcggccttactacaaaaacgctgctggccgactctggtattactacggtatggacgtttggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG1 100 100 [323] g73>t,A25>C(+ - -)|c74>g,A25>C(+ - -)|g104>c,S35>T(+ - +)|c111>g|c120>t|g161>c,G54>A(- + -)|a166>g,I56>V(+ - +)|g173>c,S58>T(+ - +)|c174>t,S58>T(+ - +)|a177>g|t183>c|g185>a,G62>D(- - -)|a190>g,T64>A(- - -)|a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|c231>t,T77>L(- - -)|a252>c,K84>N(+ - -)|c257>t,T86>I(- - -)|g258>a,T86>I(- - -)|a284>g,K95>R(+ + +)|g301>a,V101>M(+ - -)|t306>c|a313>g,T105>A(- - -)| g73>t,A25>C(+ - -)|c74>g,A25>C(+ - -)| g104>c,S35>T(+ - +)|c111>g| c120>t|g161>c,G54>A(- + -)| a166>g,I56>V(+ - +)|g173>c,S58>T(+ - +)|c174>t,S58>T(+ - +)|a177>g|t183>c|g185>a,G62>D(- - -)|a190>g,T64>A(- - -)| a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|c231>t,T77>L(- - -)|a252>c,K84>N(+ - -)|c257>t,T86>I(- - -)|g258>a,T86>I(- - -)|a284>g,K95>R(+ + +)|g301>a,V101>M(+ - -)|t306>c| a313>g,T105>A(- - -)| 320 233 210 23 5 18 5 3 5 2 2 0 0 0 3 2 1 0 78 6 4 2 0 2 0 0 0 0 0 0 0 0 0 1 1 0 36 24 22 2 1 1 0 0 0 0 0 0 0 0 1 1 0 0 51 51 49 2 1 1 0 0 1 0 0 0 0 0 1 0 0 0 30 30 23 7 2 5 3 1 1 1 0 0 0 0 1 0 0 0 117 114 105 9 1 8 1 2 3 1 2 0 0 0 0 0 0 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| tgctct ggattcactttcactaacgcgtgg atgagttgggtccgccaggctccagggaaggggctggagtgggttgcccgt gttaaaactaagactgacgatggggcaaca gactacgctgcacccgtgaaaggcagattccttatctcaagagatgattcaaacaacatactgtatctgcaaatgaacagcctgagaaccgaggacacagccatgtactactgt gccacagatcggccttactacaaaaacgctgctggccgactctggtattactacggtatggacgtt ATDRPYYKNAAGRLWYYYGMDV IGHV3-15 IGHD4-11 IGHJ6 ggattcactttcactaacgcgtgg atgagttgggtccgccaggctccagggaaggggctggagtgggttgcccgt gttaaaactaagactgacgatggggcaaca gactacgctgcacccgtgaaaggcagattccttatctcaagagatgattcaaacaacatactgtatctgcaaatgaacagcctgagaaccgaggacacagccatgtactactgt gccacagatcggccttactacaaaaacgctgctggccgactctggtattactacggtatggacgtt IGG1 20 219 14 6 8 12 6 8 2 3 9 6 JY8QFUQ01C13EL 8697 productive Homsap IGHV1-8*01 F 912 92.06 197/214 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD1-26*01 F 1 8 8 11 8.8.11 [0.17.38.11] CAREGLLGSLGSW in-frame - aggatacaccttcatcagttatgatatcaattgggtgcgacaggccactggacaagggcttgagtggatgggatggatgaaccctaacagcggtaacacagggtttgcacagaggttccagggcagagtaaccatgaccaggaacatctccataaacacggcctacatggagctgaccaacctgacatctgatgacacggccgtatattattgtgcgagagaaggtctattgggcagcctaggatcttggggccagggaaccctggtcaccgtcgcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [278] t78>a|c104>t,T35>I(- - -)|c120>t|t186>c|c198>g|a200>t,Y67>F(- + -)|a209>g,K70>R(+ + +)|c228>a|c245>t,T82>I(- - -)|g254>a,S85>N(- - -)|a258>g|g275>c,S92>T(+ - +)|g278>a,S93>N(- - -)|g284>c,R95>T(- - -)|g291>t,E97>D(+ - +)|g303>a|c309>t| t78>a| c104>t,T35>I(- - -)| c120>t| t186>c| c198>g|a200>t,Y67>F(- + -)|a209>g,K70>R(+ + +)|c228>a|c245>t,T82>I(- - -)|g254>a,S85>N(- - -)|a258>g|g275>c,S92>T(+ - +)|g278>a,S93>N(- - -)|g284>c,R95>T(- - -)|g291>t,E97>D(+ - +)|g303>a|c309>t| g320>a| 319 (320) 221 (222) 204 17 (18) 8 (9) 9 2 3 (4) 4 1 0 1 1 1 2 1 1 0 78 1 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 101 13 5 8 2 3 2 0 0 1 1 0 2 1 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,118-119(FR2)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|aa,241-242(FR3)|ta,251-252(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,251-252(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|agtt,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,118-121(FR2)|aacc,169-172(CDR2)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| a ggatacaccttcatcagttatgat atcaattgggtgcgacaggccactggacaagggcttgagtggatgggatgg atgaaccctaacagcggtaacaca gggtttgcacagaggttccagggcagagtaaccatgaccaggaacatctccataaacacggcctacatggagctgaccaacctgacatctgatgacacggccgtatattattgt gcgagagaaggtctattgggcagcctaggatct AREGLLGSLGS IGHV1-8 IGHD1-26 IGHJ4 ggatacaccttcatcagttatgat atcaattgggtgcgacaggccactggacaagggcttgagtggatgggatgg atgaaccctaacagcggtaacaca gggtttgcacagaggttccagggcagagtaaccatgaccaggaacatctccataaacacggcctacatggagctgaccaacctgacatctgatgacacggccgtatattattgt gcgagagaaggtctattgggcagcctaggatct IGA1 16 213 10 6 7 12 3 4 6 1 8 1 JY8QFUQ01C160X 15519 productive (see comment) Homsap IGHV4-61*02 F 947 92.31 204/221 nt 91.86 203/221 nt Homsap IGHJ6*02 F 247 88.71 55/62 nt Homsap IGHD3-16*02 F 1 10 7 17 10.7.17 [1.17.38.11] CARGKWGDKPPFYYGMDVW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 65 of V-REGION: 'G' (1 nucleotides at position 105 to 105 in the user submitted sequence), (cause frameshift); tctctggtggctccatcaggagtggtagttactactggagctggatccggcagcccgccgggaagggactggagtggattgggcgtatatatagcagtgggagcGatcgcacgcaacccctccctcaagagccgagtcaccatatcaattgacacgtccaagaaccaggtctccctgaaactgggctctgtgaccgccgcagacacggccgtctattattgtgcgagagggaagtggggggacaaaccgcccttctactacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [305] c93>g,S31>R(- - -)|c168>a|c173>g,T58>S(+ - +)|c194>t,T65>I(- - -)|a196>g,N66>A(- - -)|a197>c,N66>A(- - -)|c198>a,N66>A(- - -)|t199>c,Y67>R(- - -)|a200>g,Y67>R(- - -)|t222>c|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|t259>g,F87>V(+ - -)|g270>a|a274>g,S92>G(+ + -)|g303>c|c309>t| c93>g,S31>R(- - -)| c168>a|c173>g,T58>S(+ - +)|c194>t,T65>I(- - -)| a196>g,N66>A(- - -)|a197>c,N66>A(- - -)|c198>a,N66>A(- - -)|t199>c,Y67>R(- - -)|a200>g,Y67>R(- - -)|t222>c|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|t259>g,F87>V(+ - -)|g270>a|a274>g,S92>G(+ + -)|g303>c|c309>t| a320>g| 319 (320) 228 (229) 211 17 (18) 5 (6) 12 3 (4) 2 2 2 1 2 1 0 1 2 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 29 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 18 3 1 2 0 0 1 0 0 1 0 0 0 1 0 0 117 114 101 13 4 9 3 2 1 2 1 1 1 0 1 0 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcaggagtggtagttactac tggagctggatccggcagcccgccgggaagggactggagtggattgggcgt atatatagcagtgggagcatc gcacgcaacccctccctcaagagccgagtcaccatatcaattgacacgtccaagaaccaggtctccctgaaactgggctctgtgaccgccgcagacacggccgtctattattgt gcgagagggaagtggggggacaaaccgcccttctactacggtatggacgtc ARGKWGDKPPFYYGMDV IGHV4-61 IGHD3-16 IGHJ6 ggtggctccatcaggagtggtagttactac tggagctggatccggcagcccgccgggaagggactggagtggattgggcgt atatatagcagtgggagcatc gcacgcaacccctccctcaagagccgagtcaccatatcaattgacacgtccaagaaccaggtctccctgaaactgggctctgtgaccgccgcagacacggccgtctattattgt gcgagagggaagtggggggacaaaccgcccttctactacggtatggacgtc IGG1 17 216 9 8 4 9 5 8 4 1 9 3 JY8QFUQ01C17HE 18006 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 819 86.64 188/217 nt 86.18 187/217 nt Homsap IGHJ6*02 F (see comment) 193 79.03 49/62 nt Homsap IGHD6-6*01 F 1 8 8 17 8.8.17 [1.17.38.11] CVRDSGPSGYARYESMDVW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) in FR3-IMGT at codon 72 of V-REGION: 'G' (1 nucleotides at position 123 to 123 in the user submitted sequence), (cause frameshift); ctctggattcagcctcacttcctatggcatgaactgggtccgccaggctccagggaggggcgtggagtgggtctcacacgttaatatgggtagtactcacatatactacgtaggctccgtgaGggggccgattcaccatctccagagacgacgccaagaactcagtgtatctgcagatgaacaacttgagagccgaggacacggctctatattactgtgtgagagatagtggcccgtccggctacgctaggtacgagtctatggacgtctggggccaagggaccgcggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG2 100 100 [301] c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|g104>c,S35>T(+ - +)|a106>t|g107>c|a112>g,S38>G(+ + -)|a143>g,K48>R(+ + +)|g147>c|c148>g,L50>V(+ - +)|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)|a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|g173>t,S58>M(- - -)|t174>g,S58>M(- - -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)|c203>t,A68>V(+ - +)|a206>g,D69>G(- - -)|a210>c|a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|c259>g,L87>V(+ - +)|a270>g|g278>a,S93>N(- - -)|c280>t|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)|c314>t,A105>V(+ - +)| c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|g104>c,S35>T(+ - +)|a106>t|g107>c|a112>g,S38>G(+ + -)| a143>g,K48>R(+ + +)|g147>c|c148>g,L50>V(+ - +)|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)| a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|g173>t,S58>M(- - -)|t174>g,S58>M(- - -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)| c203>t,A68>V(+ - +)|a206>g,D69>G(- - -)|a210>c|a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|c259>g,L87>V(+ - +)|a270>g|g278>a,S93>N(- - -)|c280>t|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| c314>t,A105>V(+ - +)| 320 225 195 30 6 24 8 3 3 3 1 1 1 0 5 3 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 2 4 1 0 0 1 0 0 1 0 2 1 0 0 51 51 46 5 1 4 1 0 0 1 0 1 0 0 1 1 0 0 30 24 17 7 0 7 2 1 0 1 0 0 0 0 1 0 1 1 117 114 103 11 3 8 4 2 2 0 1 0 0 0 1 1 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagcctcacttcctatggc atgaactgggtccgccaggctccagggaggggcgtggagtgggtctcacac gttaatatgggtagtactcacata tactacgtaggctccgtgaggggccgattcaccatctccagagacgacgccaagaactcagtgtatctgcagatgaacaacttgagagccgaggacacggctctatattactgt gtgagagatagtggcccgtccggctacgctaggtacgagtctatggacgtc VRDSGPSGYARYESMDV IGHV3-21 IGHD6-6 IGHJ6 ggattcagcctcacttcctatggc atgaactgggtccgccaggctccagggaggggcgtggagtgggtctcacac gttaatatgggtagtactcacata tactacgtaggctccgtgaggggccgattcaccatctccagagacgacgccaagaactcagtgtatctgcagatgaacaacttgagagccgaggacacggctctatattactgt gtgagagatagtggcccgtccggctacgctaggtacgagtctatggacgtc IGG2 29 213 16 13 5 15 11 14 4 2 12 11 JY8QFUQ01C185F 7819 productive (see comment) Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F or Homsap IGHV3-15*07 F 818 85.27 191/224 nt 84.82 190/224 nt Homsap IGHJ5*02 F 110 69.39 34/49 nt Homsap IGHD1-26*01 F 2 8 10 5 8.10.5 [1.17.38.11] CREAIAW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 58 to 58 in the user submitted sequence), (cause frameshift); cctctgggttcgtgttcggtaacttctttatgaattggttccgccagcctccgggaaAaggggctggagtgggttggccgcatcaaaaccaaagttgatggtgagacaacagactacgctgcagccgtgaaagacagattcatcatttcaagagatgattcaaaaaatacgatgtatctgcaaatgaacgacctgaagaccgaggacacggccgtatattattgtcgggaggccatcgcgtggggccagggaaccctggtcaaggtctcatcggcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [271] a81>g|a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|t87>g,T29>V(- - -)|a103>g,S35>G(+ + -)|g109>t,A37>F(+ - -)|c110>t,A37>F(+ - -)|g113>t,W38>F(+ + -)|g114>t,W38>F(+ + -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g124>t,V42>F(+ - -)|g133>c,A45>P(- - -)|a138>g|g141>a|t165>c|t168>c|g173>c,S58>T(+ - +)|a178>g,T60>V(- - -)|c179>t,T60>V(- - -)|g188>a,G63>E(- - -)|c208>g,P70>A(- - -)|g221>a,G74>D(- - -)|c230>t,T77>I(- - -)|c234>t|c255>t|c259>a,L87>M(+ + -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|a285>g|a297>g|g303>a|c309>t| a81>g|a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|t87>g,T29>V(- - -)|a103>g,S35>G(+ + -)|g109>t,A37>F(+ - -)|c110>t,A37>F(+ - -)|g113>t,W38>F(+ + -)|g114>t,W38>F(+ + -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g124>t,V42>F(+ - -)|g133>c,A45>P(- - -)|a138>g|g141>a|t165>c| t168>c|g173>c,S58>T(+ - +)|a178>g,T60>V(- - -)|c179>t,T60>V(- - -)|g188>a,G63>E(- - -)| c208>g,P70>A(- - -)|g221>a,G74>D(- - -)|c230>t,T77>I(- - -)|c234>t|c255>t|c259>a,L87>M(+ + -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|a285>g|a297>g|g303>a|c309>t| a313>c,T105>R(- - -)|c314>g,T105>R(- - -)|c315>g,T105>R(- - -)|a316>g,T106>E(- - -)|c317>a,T106>E(- - -)|a318>g,T106>E(- - -)|a320>c| 312 (320) 224 (232) 191 (192) 33 (40) 11 (12) 22 (28) 8 (10) 6 8 2 0 (2) 1 (2) 0 0 2 1 (3) 4 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 15 9 1 8 3 0 2 0 0 0 0 0 0 0 3 1 51 51 44 7 3 4 1 2 1 1 0 0 0 0 1 0 1 0 30 30 25 5 1 4 1 1 1 1 0 0 0 0 1 0 0 0 117 114 102 12 6 6 3 3 4 0 0 1 0 0 0 1 0 0 0 (8) 0 0 (1) 0 (7) 0 (1) 0 (6) 0 (2) 0 0 0 0 (2) 0 (1) 0 0 0 0 (2) 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| cctct gggttcgtgttcggtaacttcttt atgaattggttccgccagcctccgggaaaggggctggagtgggttggccgc atcaaaaccaaagttgatggtgagacaaca gactacgctgcagccgtgaaagacagattcatcatttcaagagatgattcaaaaaatacgatgtatctgcaaatgaacgacctgaagaccgaggacacggccgtatattattgt cgggaggccatcgcg REAIA IGHV3-15 IGHD1-26 IGHJ5 gggttcgtgttcggtaacttcttt atgaattggttccgccagcctccgggaaaggggctggagtgggttggccgc atcaaaaccaaagttgatggtgagacaaca gactacgctgcagccgtgaaagacagattcatcatttcaagagatgattcaaaaaatacgatgtatctgcaaatgaacgacctgaagaccgaggacacggccgtatattattgt cgggaggccatcgcg IGA1 33 219 24 9 14 22 10 11 9 2 10 12 JY8QFUQ01C1QUY 10113 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 855 88.48 192/217 nt NA Homsap IGHJ5*02 F 201 88.24 45/51 nt Homsap IGHD1-26*01 F 3 8 8 12 8.8.12 [1.17.38.11] CARDGGSYNRMDLW in-frame + gtctgggttcagtttcattgactatggcatacactgggtccgccaggctccaggcaaggggctggagtggatggcaattatatggtatgatgggaaaaataaatattatgaagagtccgtgaaggaccgattcaccgtctccagagacaattccaagaacacggtgtatttggaagtgaatagtctgagagtcgacgacacggctgtgtattactgtgcgagagacggtgggagctacaacaggatggacctttggggccagggaaccctggtcaccgtctcgtcagcatccccggaccagccccaaagggtcttcccgctgagcctcgacagcaccccccaaaggatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 74 [287, 289] a81>g|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>t,S35>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g117>a,M39>I(+ + -)|g157>a,V53>M(+ - -)|g163>a,V55>I(+ - +)|a186>g|g188>a,S63>K(- - -)|t189>a,S63>K(- - -)|c198>t|c203>a,A68>E(- - -)|c207>g,D69>E(+ - +)|g221>a,G74>D(- - -)|a232>g,I78>V(+ - +)|c259>g,L87>V(+ - +)|c265>t|c268>g,Q90>E(+ + -)|a271>g,M91>V(+ - -)|c276>t|c279>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| a81>g|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>t,S35>I(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g117>a,M39>I(+ + -)|g157>a,V53>M(+ - -)|g163>a,V55>I(+ - +)| a186>g|g188>a,S63>K(- - -)|t189>a,S63>K(- - -)| c198>t|c203>a,A68>E(- - -)|c207>g,D69>E(+ - +)|g221>a,G74>D(- - -)|a232>g,I78>V(+ - +)|c259>g,L87>V(+ - +)|c265>t|c268>g,Q90>E(+ + -)|a271>g,M91>V(+ - -)|c276>t|c279>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| 320 225 200 25 6 19 5 6 6 0 0 1 0 1 1 4 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 1 5 2 1 1 0 0 0 0 0 0 1 1 0 51 51 48 3 0 3 0 3 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 1 1 0 0 0 0 0 1 0 0 0 0 117 114 101 13 4 9 2 1 5 0 0 1 0 0 1 3 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct gggttcagtttcattgactatggc atacactgggtccgccaggctccaggcaaggggctggagtggatggcaatt atatggtatgatgggaaaaataaa tattatgaagagtccgtgaaggaccgattcaccgtctccagagacaattccaagaacacggtgtatttggaagtgaatagtctgagagtcgacgacacggctgtgtattactgt gcgagagacggtgggagctacaacaggatggacctt ARDGGSYNRMDL IGHV3-33 IGHD1-26 IGHJ5 gggttcagtttcattgactatggc atacactgggtccgccaggctccaggcaaggggctggagtggatggcaatt atatggtatgatgggaaaaataaa tattatgaagagtccgtgaaggaccgattcaccgtctccagagacaattccaagaacacggtgtatttggaagtgaatagtctgagagtcgacgacacggctgtgtattactgt gcgagagacggtgggagctacaacaggatggacctt IGA2 25 213 17 8 12 19 5 6 4 2 12 7 JY8QFUQ01C1UWI 3771 productive Homsap IGHV3-11*01 F 936 92.63 201/217 nt NA Homsap IGHJ4*02 F 141 77.08 37/48 nt Homsap IGHD5-18*01 F 3 8 8 10 8.8.10 [1.17.38.11] CANELGYCSGGG in-frame + ctctggattcaccttcagtgactactacatgaactggatccgccaggctccagggaaggggctggagtggctttcatacattagtggtagtggaactaccatatactacgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacactgtatttacaaatgtacagcctgagagtcgaggacacggccgtatattactgtgcgaacgaactcggatactgtagtggtgggggggcccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaagggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttc NA IGA1 100 95 [279, 278] g119>a,S40>N(- - -)|g157>c,V53>L(+ - +)|a172>g,S58>G(+ + -)|t186>a|g188>c,S63>T(+ - +)|t210>c|a225>c|g240>a|c246>t|g247>t,A83>S(- + -)|t256>a,S86>T(+ - +)|c265>t|g267>a|a274>t,N92>Y(- - -)|c287>t,A96>V(+ - +)|g303>a| g119>a,S40>N(- - -)|g157>c,V53>L(+ - +)| a172>g,S58>G(+ + -)|t186>a|g188>c,S63>T(+ - +)| t210>c|a225>c|g240>a|c246>t|g247>t,A83>S(- + -)|t256>a,S86>T(+ - +)|c265>t|g267>a|a274>t,N92>Y(- - -)|c287>t,A96>V(+ - +)|g303>a| g317>a,R106>N(+ - -)|a318>c,R106>N(+ - -)| 316 (320) 221 (225) 205 (207) 16 (18) 8 8 (10) 1 4 (5) 3 1 1 (2) 0 1 2 2 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 30 24 21 3 1 2 1 0 0 0 0 0 0 1 1 0 0 0 117 114 103 11 7 4 0 3 3 1 1 0 1 1 0 0 1 0 4 (8) 4 4 (6) 0 (2) 0 0 (2) 0 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgactactac atgaactggatccgccaggctccagggaaggggctggagtggctttcatac attagtggtagtggaactaccata tactacgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacactgtatttacaaatgtacagcctgagagtcgaggacacggccgtatattactgt gcgaacgaactcggatactgtagtggtggg ANELGYCSGG IGHV3-11 IGHD5-18 IGHJ4 ggattcaccttcagtgactactac atgaactggatccgccaggctccagggaaggggctggagtggctttcatac attagtggtagtggaactaccata tactacgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacactgtatttacaaatgtacagcctgagagtcgaggacacggccgtatattactgt gcgaacgaactcggatactgtagtggtggg IGA1 16 213 9 7 7 10 2 6 7 1 6 2 JY8QFUQ01C1WLX 17577 productive (see comment) Homsap IGHV1-8*01 F 948 93.93 201/214 nt 93.46 200/214 nt Homsap IGHJ1*01 F 188 84.62 44/52 nt Homsap IGHD4-23*01 ORF 2 8 8 13 8.8.13 [0.17.38.11] CARGSLDYGGLPQHW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 53 of V-REGION: 'A' (1 nucleotides at position 69 to 69 in the user submitted sequence), (cause frameshift); tggatacaccttcaccagttatgatatcaactgggtgcgacaggccgctggacaagggcttgagtggaAtgggatgggtgaaccctaatagtggtgccacaggctatgcacagaagttccagggcagagtcaccatgagcagggacacctccgaaagtacagcctacgtggagctgagcagcctgggatctgaggacacggccgtgtatttctgtgcgagaggctccctggactacggtggcctcccccagcactggggccagggcaccctggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [285, 284] a136>g,T46>A(- - -)|a166>g,M56>V(+ - -)|c177>t|a190>g,N64>A(- - -)|a191>c,N64>A(- - -)|c236>g,T79>S(+ - +)|a241>g,N81>D(+ + -)|a250>g,I84>E(- - -)|t251>a,I84>E(- - -)|c255>t|a265>g,M89>V(+ - -)|a283>g,R95>G(- - -)|a308>t,Y103>F(- + -)| a136>g,T46>A(- - -)| a166>g,M56>V(+ - -)|c177>t|a190>g,N64>A(- - -)|a191>c,N64>A(- - -)| c236>g,T79>S(+ - +)|a241>g,N81>D(+ + -)|a250>g,I84>E(- - -)|t251>a,I84>E(- - -)|c255>t|a265>g,M89>V(+ - -)|a283>g,R95>G(- - -)|a308>t,Y103>F(- + -)| 320 222 209 13 2 11 7 0 2 0 1 0 1 1 0 1 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 2 0 1 0 1 0 0 0 0 0 0 0 117 114 106 8 1 7 4 0 1 0 0 0 1 1 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,118-119(FR2)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|aa,241-242(FR3)|ta,251-252(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,251-252(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|agtt,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,118-121(FR2)|aacc,169-172(CDR2)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcaccagttatgat atcaactgggtgcgacaggccgctggacaagggcttgagtggatgggatgg gtgaaccctaatagtggtgccaca ggctatgcacagaagttccagggcagagtcaccatgagcagggacacctccgaaagtacagcctacgtggagctgagcagcctgggatctgaggacacggccgtgtatttctgt gcgagaggctccctggactacggtggcctcccccagcac ARGSLDYGGLPQH IGHV1-8 IGHD4-23 IGHJ1 ggatacaccttcaccagttatgat atcaactgggtgcgacaggccgctggacaagggcttgagtggatgggatgg gtgaaccctaatagtggtgccaca ggctatgcacagaagttccagggcagagtcaccatgagcagggacacctccgaaagtacagcctacgtggagctgagcagcctgggatctgaggacacggccgtgtatttctgt gcgagaggctccctggactacggtggcctcccccagcac IGA2 13 213 9 4 2 3 7 10 1 1 8 3 JY8QFUQ01C24ZT 1106 productive Homsap IGHV3-9*01 F 1026 97.24 211/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD1-20*01 F 3 8 8 13 8.8.13 [1.17.38.11] CAKDMGYTWKYIDHW in-frame + ctctggattcacctttgatgattatgccatgcactgggtccggcaagctccagggaagggcctggagtgggtctcaggtattacttggaacagtggtaggataggctatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgcgacctgaggacacggccttgtattattgtgcaaaagatatggggtatacctggaagtacattgaccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA1 71 100 [287] g170>c,S57>T(+ - +)|t177>c|c192>g,S64>R(- - -)|a283>c|g286>c,A96>P(- - -)|c309>t| g170>c,S57>T(+ - +)|t177>c|c192>g,S64>R(- - -)| a283>c|g286>c,A96>P(- - -)|c309>t| 322 227 221 6 3 3 0 0 1 1 1 0 0 0 2 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 0 0 0 1 0 0 0 0 1 1 0 0 117 114 111 3 2 1 0 0 1 0 1 0 0 0 1 0 0 0 10 10 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttgatgattatgcc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaggt attacttggaacagtggtaggata ggctatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgcgacctgaggacacggccttgtattattgt gcaaaagatatggggtatacctggaagtacattgaccac AKDMGYTWKYIDH IGHV3-9 IGHD1-20 IGHJ4 ggattcacctttgatgattatgcc atgcactgggtccggcaagctccagggaagggcctggagtgggtctcaggt attacttggaacagtggtaggata ggctatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgcgacctgaggacacggccttgtattattgt gcaaaagatatggggtatacctggaagtacattgaccac IGA1 6 213 2 4 1 4 1 2 2 1 1 2 JY8QFUQ01C2553 22092 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 873 89.4 194/217 nt NA Homsap IGHJ5*02 F 228 94.12 48/51 nt Homsap IGHD6-6*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARGRATGLVDWFDPW in-frame - ctctggattcaccttcaccaactacgccatgacctgggtccgccaggctccagggaaggggctggagtggatctcgactgttgtgggtggcggtggtaacacatactacgcagactccgtgaagggccggttcaccatctccagagacaattcccagaacacgctgtatttgcaaatgtacaatttgggagccgaggacacggccctatattactgtgcgagaggtagagcgactggtctggtggactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcctccaccaaggggcccatcggtcttcccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 94 [293, 292] t90>c|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|t111>c|g119>c,S40>T(+ - +)|g157>a,V53>I(+ - +)|a162>g|g163>a,A55>T(- - -)|a166>g,I56>V(+ - +)|a169>g,S57>V(- - -)|g170>t,S57>V(- - -)|t171>g,S57>V(- - -)|a175>g,S59>G(+ + -)|t177>c,S59>G(+ + -)|g191>a,S64>N(- - -)|a250>c,K84>Q(+ - -)|c265>t|a274>t,N92>Y(- - -)|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|c280>t|a283>g,R95>G(- - -)|g301>c,V101>L(+ - +)|a317>g,K106>R(+ + +)| t90>c|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|t111>c| g119>c,S40>T(+ - +)|g157>a,V53>I(+ - +)|a162>g|g163>a,A55>T(- - -)| a166>g,I56>V(+ - +)|a169>g,S57>V(- - -)|g170>t,S57>V(- - -)|t171>g,S57>V(- - -)|a175>g,S59>G(+ + -)|t177>c,S59>G(+ + -)|g191>a,S64>N(- - -)| a250>c,K84>Q(+ - -)|c265>t|a274>t,N92>Y(- - -)|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|c280>t|a283>g,R95>G(- - -)|g301>c,V101>L(+ - +)| a317>g,K106>R(+ + +)|a320>g| 319 (320) 224 (225) 200 24 (25) 5 (6) 19 6 (7) 5 3 3 1 0 1 0 3 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 1 0 2 0 0 0 0 1 0 0 0 51 51 47 4 1 3 1 2 0 0 0 0 0 0 1 0 0 0 30 24 17 7 0 7 3 1 0 1 0 0 0 0 0 0 1 1 117 114 106 8 2 6 1 1 3 0 1 0 1 0 1 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 1 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcaccaactacgcc atgacctgggtccgccaggctccagggaaggggctggagtggatctcgact gttgtgggtggcggtggtaacaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattcccagaacacgctgtatttgcaaatgtacaatttgggagccgaggacacggccctatattactgt gcgagaggtagagcgactggtctggtggactggttcgacccc ARGRATGLVDWFDP IGHV3-23 IGHD6-6 IGHJ5 ggattcaccttcaccaactacgcc atgacctgggtccgccaggctccagggaaggggctggagtggatctcgact gttgtgggtggcggtggtaacaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattcccagaacacgctgtatttgcaaatgtacaatttgggagccgaggacacggccctatattactgt gcgagaggtagagcgactggtctggtggactggttcgacccc IGG2 23 213 16 7 8 12 8 11 3 2 9 9 JY8QFUQ01C26ES 4458 productive (see comment) Homsap IGHV4-4*07 F 844 88.73 189/213 nt 87.79 187/213 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-19*01 F 1 8 7 11 8.7.11 [1.17.37.11] CARSGWDNQFDFW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'TGGCAC' (6 nucleotides at position 22 to 27 in the user submitted sequence), (do not cause frameshift); in FR3-IMGT, from codon 68 of V-REGION: 1 nucleotides (from position 109 in the user submitted sequence), (cause frameshift) ctctggtgtcgccaccagtagTGGCACttactactggagctggatccggcagtccgccggggcgggactagagtggattgggcgcatctataccggtcacaccaccatttacaaccctccctcaagggtcgagtcaccatgtcacttgacatgtccaagaaccagatctccctgaggctgacctctgtgaccgccgcagatacggccgtgtattactgtgcgcgaagtggctgggacaatcaatttgacttctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [284] g83>t,G28>V(- - -)|t85>g,S29>A(- + -)|t89>c,I30>T(- - -)|c133>t,P45>S(+ - -)|a142>g,K48>A(- - -)|a143>c,K48>A(- - -)|g150>a|t165>c|a175>g,S59>G(+ + -)|g187>c,G63>H(+ - -)|g188>a,G63>H(+ - -)|g189>c,G63>H(+ - -)|g191>c,S64>T(+ - +)|a197>t,N66>I(- - -)|c198>t,N66>I(- - -)|a220>g,S74>G(+ + -)|g238>c,V80>L(+ - +)|a240>t,V80>L(+ - +)|c245>t,T82>M(- - -)|t259>a,F87>I(+ - -)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|g291>a|c294>t|a316>c| g83>t,G28>V(- - -)|t85>g,S29>A(- + -)|t89>c,I30>T(- - -)| c133>t,P45>S(+ - -)|a142>g,K48>A(- - -)|a143>c,K48>A(- - -)|g150>a|t165>c| a175>g,S59>G(+ + -)|g187>c,G63>H(+ - -)|g188>a,G63>H(+ - -)|g189>c,G63>H(+ - -)|g191>c,S64>T(+ - +)| a197>t,N66>I(- - -)|c198>t,N66>I(- - -)|a220>g,S74>G(+ + -)|g238>c,V80>L(+ - +)|a240>t,V80>L(+ - +)|c245>t,T82>M(- - -)|t259>a,F87>I(+ - -)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|g291>a|c294>t| a316>c|g319>a|a320>g| 318 (320) 219 (221) 194 25 (27) 5 (7) 20 4 (5) 3 (4) 4 2 2 0 2 1 5 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 1 0 0 0 0 0 0 1 1 51 51 46 5 2 3 1 1 1 1 1 0 0 0 0 0 0 0 30 21 16 5 0 5 1 1 0 0 0 0 0 0 3 0 0 0 117 113 102 11 2 9 2 1 3 0 0 0 2 1 2 0 0 0 6 (8) 6 5 1 (3) 1 (3) 0 0 (1) 0 (1) 0 0 1 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtgtcgccaccagtagttactac tggagctggatccggcagtccgccggggcgggactagagtggattgggcgc atctataccggtcacaccacc atttacaaccctccctcaagggtcgagtcaccatgtcacttgacatgtccaagaaccagatctccctgaggctgacctctgtgaccgccgcagatacggccgtgtattactgt gcgcgaagtggctgggacaatcaatttgacttc ARSGWDNQFDF IGHV4-4 IGHD6-19 IGHJ4 ggtgtcgccaccagtagttactac tggagctggatccggcagtccgccggggcgggactagagtggattgggcgc atctataccggtcacaccacc atttacaaccctccctcaagggtcgagtcaccatgtcacttgacatgtccaagaaccagatctccctgaggctgacctctgtgaccgccgcagatacggccgtgtattactgt gcgcgaagtggctgggacaatcaatttgacttc IGG1 24 209 13 11 7 13 6 11 4 0 12 8 JY8QFUQ01C28D8 9034 productive Homsap IGHV1-8*01 F 939 93.46 200/214 nt NA Homsap IGHJ6*02 F 112 64.52 40/62 nt Homsap IGHD5-24*01 ORF 3 8 8 16 8.8.16 [0.17.38.11] CARGYGYPNYYHYAMDVW in-frame + tggataccccttcgacagttatgatatcagctgggtgcgacaggccactggacaagggcttgagtggatgggatggatgaaccctaacagtgggaatacagcctatgcacagaagttccagggccgagtcacgatgaccagggacacctccacaagcacagcctacatggaggtgagcagcctcagatctgaggacacggccgtctattactgtgcgcgcggttatggctacccaaactactaccactacgctatggacgtctggggccaagggttccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 100 [294] a85>c,T29>P(+ + -)|a103>g,T35>D(- + -)|c104>a,T35>D(- + -)|a119>g,N40>S(- - -)|t189>g|c192>t|g197>c,G66>A(- + -)|a223>c|c231>g|a241>g,N81>D(+ + -)|t251>c,I84>T(- - -)|c271>g,L91>V(+ - +)|g282>c|g303>c|a316>c|a318>c| a85>c,T29>P(+ + -)|a103>g,T35>D(- + -)|c104>a,T35>D(- + -)| a119>g,N40>S(- - -)| t189>g|c192>t| g197>c,G66>A(- + -)|a223>c|c231>g|a241>g,N81>D(+ + -)|t251>c,I84>T(- - -)|c271>g,L91>V(+ - +)|g282>c|g303>c| a316>c|a318>c| 320 222 206 16 8 8 3 0 1 1 4 1 0 0 3 2 0 1 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 0 0 0 1 1 0 0 0 0 0 0 51 51 50 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 2 0 0 0 1 0 0 0 0 0 0 0 0 1 117 114 106 8 4 4 1 0 0 1 1 0 0 0 3 2 0 0 8 8 6 2 2 0 0 0 0 0 2 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,118-119(FR2)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|aa,241-242(FR3)|ta,251-252(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,251-252(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|agtt,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,118-121(FR2)|aacc,169-172(CDR2)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggataccccttcgacagttatgat atcagctgggtgcgacaggccactggacaagggcttgagtggatgggatgg atgaaccctaacagtgggaataca gcctatgcacagaagttccagggccgagtcacgatgaccagggacacctccacaagcacagcctacatggaggtgagcagcctcagatctgaggacacggccgtctattactgt gcgcgcggttatggctacccaaactactaccactacgctatggacgtc ARGYGYPNYYHYAMDV IGHV1-8 IGHD5-24 IGHJ6 ggataccccttcgacagttatgat atcagctgggtgcgacaggccactggacaagggcttgagtggatgggatgg atgaaccctaacagtgggaataca gcctatgcacagaagttccagggccgagtcacgatgaccagggacacctccacaagcacagcctacatggaggtgagcagcctcagatctgaggacacggccgtctattactgt gcgcgcggttatggctacccaaactactaccactacgctatggacgtc IGA1 14 213 5 9 1 7 4 7 4 2 5 3 JY8QFUQ01C2A2E 7233 productive (see comment) Homsap IGHV3-13*01 F 904 91.2 197/216 nt 90.74 196/216 nt Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD4-11*01 ORF 3 8 7 17 8.7.17 [2.17.38.11] CAREVTRLTVPYYLYFDLW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 97 of V-REGION: 'G' (1 nucleotides at position 193 to 193 in the user submitted sequence), (cause frameshift); gtctctggaatcaccttcagtgactccgacatgcactgggtccgccaagctccaggagaaggtctggagtgggtcgcagctattggaactgctggtgatacatactatgcagactccgtgaagggccgattctccattaccagagagaatgccaagaactccttgtttcttcaaatgaacagcctgagagccGggggacacggctgtttattattgtgcaagagaggtgactagacttactgtcccctattacttatacttcgatctctggggccgtggcaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [299] c74>t,A25>V(+ - +)|t82>a,F28>I(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>c,Y37>S(+ - -)|a136>c,T46>P(+ + -)|a142>g,K48>E(+ - -)|t160>g,S54>A(- + -)|t171>a|c192>t|c202>g,P68>A(- - -)|g206>a,G69>D(- - -)|a229>t,T77>S(+ - +)|c234>t|t235>a,S79>T(+ - +)|a243>g|a263>t,Y88>F(- + -)|g303>t|c309>t| c74>t,A25>V(+ - +)| t82>a,F28>I(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>c,Y37>S(+ - -)| a136>c,T46>P(+ + -)|a142>g,K48>E(+ - -)|t160>g,S54>A(- + -)| t171>a|c192>t| c202>g,P68>A(- - -)|g206>a,G69>D(- - -)|a229>t,T77>S(+ - +)|c234>t|t235>a,S79>T(+ - +)|a243>g|a263>t,Y88>F(- + -)|g303>t|c309>t| 320 224 205 19 6 13 3 2 4 0 2 0 2 3 0 1 1 1 78 6 5 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 0 0 1 0 0 1 0 0 0 0 51 51 48 3 0 3 1 0 0 0 1 0 0 0 0 0 0 1 30 21 19 2 2 0 0 0 1 0 0 0 0 1 0 0 0 0 117 114 105 9 4 5 1 1 2 0 0 0 2 1 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|ta,135-136(FR2)|aa,141-142(FR2)|aa,143-144(FR2)|ta,165-166(FR2-CDR2)|ta,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,242-243(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,135-136(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,259-260(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|agct,162-165(FR2)|ggta,169-172(CDR2)|ggct,205-208(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,162-165(FR2)|tact,171-174(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtctct ggaatcaccttcagtgactccgac atgcactgggtccgccaagctccaggagaaggtctggagtgggtcgcagct attggaactgctggtgataca tactatgcagactccgtgaagggccgattctccattaccagagagaatgccaagaactccttgtttcttcaaatgaacagcctgagagccggggacacggctgtttattattgt gcaagagaggtgactagacttactgtcccctattacttatacttcgatctc AREVTRLTVPYYLYFDL IGHV3-13 IGHD4-11 IGHJ2 ggaatcaccttcagtgactccgac atgcactgggtccgccaagctccaggagaaggtctggagtgggtcgcagct attggaactgctggtgataca tactatgcagactccgtgaagggccgattctccattaccagagagaatgccaagaactccttgtttcttcaaatgaacagcctgagagccggggacacggctgtttattattgt gcaagagaggtgactagacttactgtcccctattacttatacttcgatctc IGA2 18 210 8 10 5 7 3 11 4 2 8 4 JY8QFUQ01C2JVO 21366 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 932 92.2 201/218 nt NA Homsap IGHJ6*02 F 202 80.65 50/62 nt Homsap IGHD3-16*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARHNYGNEKYIMDVW in-frame + gttttggatacaactttgccacctattggatcggctgggtgcgccacatgcccgggaaaggcctggaatggatggggatgatctttgctggtgactctgacaccagatacagtccgtccttccgaggccaggtcaccatgtcagccgacaagtccatcaacaccgcctacctgcagtggagcagcctgatggcctcggacaccgccatatattactgtgcgagacataattatggaaacgagaagtacattatggacgtctggggccaggggaccacggtcaccgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [291] c77>t,S26>F(- - -)|g86>a,S29>N(- - -)|a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)|c111>t|g132>c,Q44>H(- + -)|g153>a|c165>g,I55>M(+ + -)|a170>t,Y57>F(- + -)|c172>g,P58>A(- - -)|t192>c|c204>t|a215>g,Q72>R(+ - -)|c234>g,I78>M(+ + -)|g254>a,S85>N(- - -)|a284>t,K95>M(- + -)|g303>a,M101>I(+ + -)| c77>t,S26>F(- - -)| g86>a,S29>N(- - -)|a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)|c111>t| g132>c,Q44>H(- + -)|g153>a|c165>g,I55>M(+ + -)| a170>t,Y57>F(- + -)|c172>g,P58>A(- - -)|t192>c| c204>t|a215>g,Q72>R(+ - -)|c234>g,I78>M(+ + -)|g254>a,S85>N(- - -)|a284>t,K95>M(- + -)|g303>a,M101>I(+ + -)| 320 226 209 17 4 13 2 4 3 1 0 0 2 0 2 3 0 0 78 5 4 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 1 0 0 0 0 0 1 0 0 0 51 51 48 3 1 2 0 1 0 0 0 0 0 0 1 1 0 0 30 24 21 3 1 2 0 0 0 1 0 0 1 0 0 1 0 0 117 114 108 6 1 5 1 2 1 0 0 0 1 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| gtttt ggatacaactttgccacctattgg atcggctgggtgcgccacatgcccgggaaaggcctggaatggatggggatg atctttgctggtgactctgacacc agatacagtccgtccttccgaggccaggtcaccatgtcagccgacaagtccatcaacaccgcctacctgcagtggagcagcctgatggcctcggacaccgccatatattactgt gcgagacataattatggaaacgagaagtacattatggacgtc ARHNYGNEKYIMDV IGHV5-51 IGHD3-16 IGHJ6 ggatacaactttgccacctattgg atcggctgggtgcgccacatgcccgggaaaggcctggaatggatggggatg atctttgctggtgactctgacacc agatacagtccgtccttccgaggccaggtcaccatgtcagccgacaagtccatcaacaccgcctacctgcagtggagcagcctgatggcctcggacaccgccatatattactgt gcgagacataattatggaaacgagaagtacattatggacgtc IGA1 16 213 9 7 6 11 3 5 2 2 7 5 JY8QFUQ01C2XG5 16121 productive Homsap IGHV3-30*15 F 945 93.09 202/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD5-24*01 ORF 1 8 8 16 8.8.16 [1.17.38.11] CARDELPWDTIEYYFDYW in-frame + ctctggattcacctttagtagatattccatgcactgggtccgccaggctccaggcaaggggctagagtgggtggcacttatatcatacgatggaagtagaagaatctacgcagactccgtgaagggccgattcaccatctccagagacacttccaagaacacggtgtatctgcaaatgagtagcctgagacctgaggacacggctgtgtattactgtgcgagagatgagctgccttgggatacaatagaatactactttgactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 100 100 [296] c90>t|c108>a,S36>R(- - -)|g112>t,A38>S(- + -)|t114>c,A38>S(- + -)|g163>c,V55>L(+ - +)|t174>c|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)|a194>g,K65>R(+ + +)|t196>a,Y66>I(- - -)|a197>t,Y66>I(- - -)|a245>c,N82>T(- + -)|c259>g,L87>V(+ - +)|c276>t|g286>c,A96>P(- - -)| c90>t|c108>a,S36>R(- - -)|g112>t,A38>S(- + -)|t114>c,A38>S(- + -)| g163>c,V55>L(+ - +)| t174>c|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)|a194>g,K65>R(+ + +)| t196>a,Y66>I(- - -)|a197>t,Y66>I(- - -)|a245>c,N82>T(- + -)|c259>g,L87>V(+ - +)|c276>t|g286>c,A96>P(- - -)| 320 225 210 15 3 12 2 0 2 2 1 1 1 2 2 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 1 1 0 1 0 0 0 0 1 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 20 4 1 3 2 0 0 1 0 0 0 1 0 0 0 0 117 114 108 6 1 5 0 0 1 0 1 0 1 1 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agca,274-277(FR3)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtagatattcc atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcactt atatcatacgatggaagtagaaga atctacgcagactccgtgaagggccgattcaccatctccagagacacttccaagaacacggtgtatctgcaaatgagtagcctgagacctgaggacacggctgtgtattactgt gcgagagatgagctgccttgggatacaatagaatactactttgactat ARDELPWDTIEYYFDY IGHV3-30 IGHD5-24 IGHJ4 ggattcacctttagtagatattcc atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcactt atatcatacgatggaagtagaaga atctacgcagactccgtgaagggccgattcaccatctccagagacacttccaagaacacggtgtatctgcaaatgagtagcctgagacctgaggacacggctgtgtattactgt gcgagagatgagctgccttgggatacaatagaatactactttgactat IGA2 15 213 6 9 2 7 4 8 1 2 6 6 JY8QFUQ01C2XX8 9037 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 874 89.04 195/219 nt NA Homsap IGHJ4*01 F 177 85.42 41/48 nt Homsap IGHD3-16*02 F 2 8 8 17 8.8.17 [2.17.38.11] CVRDRDFLWGSYRLQLEYW in-frame - gactctggattcagatttagcagctatggcatgagctgggtccgccaggctccaaaaaaggggctggagtgggtctcaggaattagtgcgaatggtggtagtataaattatgcagactccgtgaagggccgattcatcatctccagagacaattccaagaacacattgtttctgcaaatgaatagcctgagagccgaagacacggccgtatattactgtgtgagagatcgcgatttcctgtgggggagttatcgtcttcaacttgaatattggggccaaggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [301] c74>a,A25>D(- - -)|c86>g,T29>R(- - -)|c87>a,T29>R(- - -)|c113>g,A38>G(- + -)|g139>a,G47>K(- - -)|g140>a,G47>K(- - -)|g141>a,G47>K(- - -)|c164>g,A55>G(- + -)|t165>a,A55>G(- + -)|g173>c,G58>A(- + -)|t174>g,G58>A(- + -)|g176>a,S59>N(- - -)|c192>t|c194>t,T65>I(- - -)|t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|c201>t|g225>a|c230>t,T77>I(- - -)|g258>a|c259>t|a263>t,Y88>F(- + -)|c276>t|g291>a|c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)| c74>a,A25>D(- - -)| c86>g,T29>R(- - -)|c87>a,T29>R(- - -)|c113>g,A38>G(- + -)| g139>a,G47>K(- - -)|g140>a,G47>K(- - -)|g141>a,G47>K(- - -)|c164>g,A55>G(- + -)|t165>a,A55>G(- + -)| g173>c,G58>A(- + -)|t174>g,G58>A(- + -)|g176>a,S59>N(- - -)|c192>t|c194>t,T65>I(- - -)| t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|c201>t|g225>a|c230>t,T77>I(- - -)|g258>a|c259>t|a263>t,Y88>F(- + -)|c276>t|g291>a| c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)| 320 227 201 26 7 19 1 7 8 0 0 2 1 2 1 3 0 1 78 6 5 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 0 0 1 0 0 0 2 0 0 51 51 46 5 0 5 0 3 0 0 0 0 0 1 0 1 0 0 30 24 19 5 1 4 0 1 2 0 0 0 0 0 1 0 0 1 117 114 104 10 6 4 0 3 5 0 0 0 1 1 0 0 0 0 8 8 6 2 0 2 1 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gactct ggattcagatttagcagctatggc atgagctgggtccgccaggctccaaaaaaggggctggagtgggtctcagga attagtgcgaatggtggtagtata aattatgcagactccgtgaagggccgattcatcatctccagagacaattccaagaacacattgtttctgcaaatgaatagcctgagagccgaagacacggccgtatattactgt gtgagagatcgcgatttcctgtgggggagttatcgtcttcaacttgaatat VRDRDFLWGSYRLQLEY IGHV3-23 IGHD3-16 IGHJ4 ggattcagatttagcagctatggc atgagctgggtccgccaggctccaaaaaaggggctggagtgggtctcagga attagtgcgaatggtggtagtata aattatgcagactccgtgaagggccgattcatcatctccagagacaattccaagaacacattgtttctgcaaatgaatagcctgagagccgaagacacggccgtatattactgt gtgagagatcgcgatttcctgtgggggagttatcgtcttcaacttgaatat IGA1 23 213 14 9 14 19 0 4 6 1 9 7 JY8QFUQ01C33GJ 15121 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 896 90.37 197/218 nt 88.99 194/218 nt Homsap IGHJ4*02 F 78 62.5 30/48 nt Homsap IGHD2-8*01 F 2 8 8 8 8.8.8 [1.17.38.11] CARDNYGDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 42 of V-REGION: 'G' (1 nucleotides at position 39 to 39 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 48 of V-REGION: 'G' (1 nucleotides at position 60 to 60 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 62 of V-REGION: 'G' (1 nucleotides at position 96 to 96 in the user submitted sequence), (cause frameshift); tctggagttcagccttcagtagctactggatgcactggGgtccgccaagctccagggaaGggggctggtttgggtctcacgtgttaatggtgatgGggggtaggacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgtgcaagagataattacggtgactactggggccagggaacccctggtcacagtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [277] c74>t|c77>g,S26>W(- - -)|t78>g,S26>W(- - -)|g79>a,G27>S(+ + -)|a81>t,G27>S(+ + -)|t83>c,F28>S(- - -)|c84>a,F28>S(- - -)|a85>g,T29>A(- - -)|g153>t|a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| c74>t|c77>g,S26>W(- - -)|t78>g,S26>W(- - -)| g79>a,G27>S(+ + -)|a81>t,G27>S(+ + -)|t83>c,F28>S(- - -)|c84>a,F28>S(- - -)|a85>g,T29>A(- - -)| g153>t| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 226 205 21 6 15 5 3 1 1 0 1 1 1 3 2 2 1 78 5 2 3 1 2 0 0 1 0 0 0 0 0 0 1 0 1 36 24 19 5 0 5 1 1 0 1 0 1 1 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 30 24 20 4 0 4 3 0 0 0 0 0 0 0 0 1 0 0 117 114 106 8 4 4 1 2 0 0 0 0 0 1 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tctgg agttcagccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgt gttaatggtgatgggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 agttcagccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgt gttaatggtgatgggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac IGA2 18 213 9 9 3 10 6 8 5 0 4 9 JY8QFUQ01C39LJ 13554 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30*19 F or Homsap IGHV3-30-5*01 F or Homsap IGHV3-33*05 F 927 92.17 200/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD5-18*01 F 1 8 8 16 8.8.16 [1.17.38.11] CARNELPWDTREYYFDYW in-frame - gtctggattcaccttcagtagctacggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagttgtctcatttgatggaattcttgaacactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtatatctccaaatgagtagcctgagacctgaggacacggctgtctattactgtgcgaggaatgagctgccttgggatacaagagaatactactttgactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [296] c75>g|t111>c|a166>g,I56>V(+ - +)|a168>c,I56>V(+ - +)|a173>t,Y58>F(- + -)|g188>t,S63>I(- - -)|a190>c,N64>L(- - -)|a191>t,N64>L(- - -)|a193>g,K65>E(+ - -)|t196>c,Y66>H(+ - -)|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|g267>c|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|g286>c,A96>P(- - -)|g303>c| c75>g| t111>c| a166>g,I56>V(+ - +)|a168>c,I56>V(+ - +)|a173>t,Y58>F(- + -)|g188>t,S63>I(- - -)|a190>c,N64>L(- - -)|a191>t,N64>L(- - -)|a193>g,K65>E(+ - -)| t196>c,Y66>H(+ - -)|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|g267>c|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|g286>c,A96>P(- - -)|g303>c| a318>g|g319>a| 317 (320) 222 (225) 205 (206) 17 (19) 4 (6) 13 3 (4) 1 (2) 1 2 2 0 2 0 3 2 1 0 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 17 7 0 7 2 0 0 0 2 0 2 0 0 0 1 0 117 114 106 8 2 6 1 1 1 1 0 0 0 0 3 1 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| gtct ggattcaccttcagtagctacggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt gtctcatttgatggaattcttgaa cactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtatatctccaaatgagtagcctgagacctgaggacacggctgtctattactgt gcgaggaatgagctgccttgggatacaagagaatactactttgactat ARNELPWDTREYYFDY IGHV3-30 IGHD5-18 IGHJ4 ggattcaccttcagtagctacggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt gtctcatttgatggaattcttgaa cactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtatatctccaaatgagtagcctgagacctgaggacacggctgtctattactgt gcgaggaatgagctgccttgggatacaagagaatactactttgactat IGA1 16 213 7 9 2 7 5 9 2 1 6 7 JY8QFUQ01C3FR9 20033 productive (see comment) Homsap IGHV4-31*02 F, or Homsap IGHV4-31*03 F or Homsap IGHV4-31*04 F 920 90.95 201/221 nt 90.5 200/221 nt Homsap IGHJ1*01 F 242 96.15 50/52 nt Homsap IGHD2-21*02 F 2 10 7 18 10.7.18 [1.17.38.11] CARSAYCGADCYSADYLQHW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'G' (1 nucleotides at position 17 to 17 in the user submitted sequence), (cause frameshift); tctctggtggctccgtGcagcagtaggggttactactggaactggatccgccagttcccagggaagggcctggagtggattgggaacatcttttacagtgggggcacctacgacaacccgtccctcaggagtcgaatttctatatcattagacacgtctaagaaccaattctccctgaagttgacctctatgaccgccgcggacacggccgtgtattactgtgcgagatcggcatactgtggtgctgactgctactcagctgattacctccagcactggggccagggcaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttccccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [308, 307] a88>g,I30>V(+ - +)|g103>a,G35>R(- - -)|t105>g,G35>R(- - -)|g119>a,S40>N(- - -)|c133>t,H45>F(- - -)|a134>t,H45>F(- - -)|t163>a,Y55>N(- - -)|a170>t,Y57>F(- + -)|a190>g,S64>G(+ + -)|t199>g,Y67>D(- - -)|a215>g,K72>R(+ + +)|g226>a,V76>I(+ - +)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|g238>t,V80>L(+ - +)|g258>a|c271>t|g275>c,S92>T(+ - +)|g280>a,V94>M(+ - -)|t285>c| a88>g,I30>V(+ - +)|g103>a,G35>R(- - -)|t105>g,G35>R(- - -)| g119>a,S40>N(- - -)|c133>t,H45>F(- - -)|a134>t,H45>F(- - -)|t163>a,Y55>N(- - -)| a170>t,Y57>F(- + -)|a190>g,S64>G(+ + -)| t199>g,Y67>D(- - -)|a215>g,K72>R(+ + +)|g226>a,V76>I(+ - +)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|g238>t,V80>L(+ - +)|g258>a|c271>t|g275>c,S92>T(+ - +)|g280>a,V94>M(+ - -)|t285>c| g319>t|a320>c| 318 (320) 227 (229) 207 20 (22) 3 (5) 17 3 5 3 1 0 (1) 0 3 1 1 0 1 (2) 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 1 1 0 0 0 0 0 0 0 0 0 1 51 51 47 4 0 4 0 1 1 0 0 0 1 1 0 0 0 0 30 21 19 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 117 114 103 11 3 8 1 3 2 1 0 0 1 0 1 0 1 1 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,228-229(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,69-70(FR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,227-228(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|agca,131-134(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agtt,225-228(FR3)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,69-72(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|tacc,228-231(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tgcc,285-288(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcagcagtaggggttactac tggaactggatccgccagttcccagggaagggcctggagtggattgggaac atcttttacagtgggggcacc tacgacaacccgtccctcaggagtcgaatttctatatcattagacacgtctaagaaccaattctccctgaagttgacctctatgaccgccgcggacacggccgtgtattactgt gcgagatcggcatactgtggtgctgactgctactcagctgattacctccagcac ARSAYCGADCYSADYLQH IGHV4-31 IGHD2-21 IGHJ1 ggtggctccgtcagcagtaggggttactac tggaactggatccgccagttcccagggaagggcctggagtggattgggaac atcttttacagtgggggcacc tacgacaacccgtccctcaggagtcgaatttctatatcattagacacgtctaagaaccaattctccctgaagttgacctctatgaccgccgcggacacggccgtgtattactgt gcgagatcggcatactgtggtgctgactgctactcagctgattacctccagcac IGA1 20 216 12 8 8 10 4 10 3 0 12 5 JY8QFUQ01C3HIA 13215 productive Homsap IGHV3-30*01 F 909 91.24 198/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD5-24*01 ORF 1 8 8 16 8.8.16 [1.17.38.11] CARDELPWDTIEYYFDYW in-frame + ctctggattcaacttcaggacctattctatgcactggctccgccaggctccaggcaagggactagagtgggtatcagttatttcatatgatggaactaagaagaattatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatctacaaatggacagcctgagacctgaggacacggctgtgtattactgtgcgagagatgaactgccttgggatacaatagagtattactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 100 [296] c86>a,T29>N(- + -)|t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|g112>t,A38>S(- + -)|g124>c,V42>L(+ - +)|g147>a|g159>a|g160>t,A54>S(- + -)|a168>t|g188>c,S63>T(+ - +)|t192>g,N64>K(+ - -)|a195>g|t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|c201>t|c259>g,L87>V(+ - +)|g267>a|a274>g,N92>D(+ + -)|g286>c,A96>P(- - -)| c86>a,T29>N(- + -)|t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|g112>t,A38>S(- + -)| g124>c,V42>L(+ - +)|g147>a|g159>a|g160>t,A54>S(- + -)| a168>t|g188>c,S63>T(+ - +)|t192>g,N64>K(+ - -)|a195>g| t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|c201>t|c259>g,L87>V(+ - +)|g267>a|a274>g,N92>D(+ + -)|g286>c,A96>P(- - -)| 320 225 206 19 6 13 2 3 2 0 0 1 1 1 4 1 2 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 0 0 1 0 0 1 0 1 1 51 51 47 4 2 2 0 2 0 0 0 0 0 0 1 0 1 0 30 24 20 4 2 2 1 0 0 0 0 0 1 0 1 0 0 1 117 114 107 7 2 5 1 1 2 0 0 0 0 1 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaacttcaggacctattct atgcactggctccgccaggctccaggcaagggactagagtgggtatcagtt atttcatatgatggaactaagaag aattatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatctacaaatggacagcctgagacctgaggacacggctgtgtattactgt gcgagagatgaactgccttgggatacaatagagtattactttgactac ARDELPWDTIEYYFDY IGHV3-30 IGHD5-24 IGHJ4 ggattcaacttcaggacctattct atgcactggctccgccaggctccaggcaagggactagagtgggtatcagtt atttcatatgatggaactaagaag aattatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatctacaaatggacagcctgagacctgaggacacggctgtgtattactgt gcgagagatgaactgccttgggatacaatagagtattactttgactac IGA1 19 213 7 12 5 13 2 6 4 2 7 6 JY8QFUQ01C3HRU 20211 productive Homsap IGHV3-9*01 F 855 88.48 192/217 nt NA Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD2-21*02 F 2 8 8 14 8.8.14 [1.17.38.11] CAKATTAWYSGGLDVW in-frame + ctctggattcaactttgatgattacggcatgcactgggtccggcaacgcccagggatgggcctggagtgggtcgcaggtattagttataataatggccacaaagaatatgcggactctgtgaggggccgattcaccatctctagagacaacgccaggaagtccctgtatctgcagatggacagtctgagagttgaggacacggccttgtattattgtgcaaaggctacaactgcctggtattcaggcggtctggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [290, 289] c86>a,T29>N(- + -)|t111>c|c113>g,A38>G(- + -)|g133>c,A45>R(- - -)|c134>g,A45>R(- - -)|t135>c,A45>R(- - -)|a143>t,K48>M(- + -)|t160>g,S54>A(- + -)|g173>a,W58>Y(- + -)|g174>t,W58>Y(- + -)|g185>a,S62>N(- - -)|t189>c|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|t194>a,I65>K(- + -)|g197>a,G66>E(- - -)|c198>a,G66>E(- - -)|a215>g,K72>R(+ + +)|c237>t|a251>g,K84>R(+ + +)|c255>g,N85>K(+ - -)|a270>g|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|c309>t|a318>g|a320>c,D107>A(- - -)| c86>a,T29>N(- + -)|t111>c|c113>g,A38>G(- + -)| g133>c,A45>R(- - -)|c134>g,A45>R(- - -)|t135>c,A45>R(- - -)|a143>t,K48>M(- + -)|t160>g,S54>A(- + -)| g173>a,W58>Y(- + -)|g174>t,W58>Y(- + -)|g185>a,S62>N(- - -)|t189>c|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|t194>a,I65>K(- + -)| g197>a,G66>E(- - -)|c198>a,G66>E(- - -)|a215>g,K72>R(+ + +)|c237>t|a251>g,K84>R(+ + +)|c255>g,N85>K(+ - -)|a270>g|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|c309>t| a318>g|a320>c,D107>A(- - -)| 322 227 200 27 6 21 5 4 3 3 2 2 1 1 1 3 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 0 1 0 1 0 0 0 1 0 0 51 51 46 5 0 5 0 0 0 1 0 0 1 0 1 1 0 1 30 24 17 7 1 6 0 3 0 1 1 0 0 1 0 0 1 0 117 114 104 10 3 7 4 1 3 0 0 1 0 0 0 1 0 0 10 10 8 2 1 1 1 0 0 0 1 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaactttgatgattacggc atgcactgggtccggcaacgcccagggatgggcctggagtgggtcgcaggt attagttataataatggccacaaa gaatatgcggactctgtgaggggccgattcaccatctctagagacaacgccaggaagtccctgtatctgcagatggacagtctgagagttgaggacacggccttgtattattgt gcaaaggctacaactgcctggtattcaggcggtctggacgtc AKATTAWYSGGLDV IGHV3-9 IGHD2-21 IGHJ6 ggattcaactttgatgattacggc atgcactgggtccggcaacgcccagggatgggcctggagtgggtcgcaggt attagttataataatggccacaaa gaatatgcggactctgtgaggggccgattcaccatctctagagacaacgccaggaagtccctgtatctgcagatggacagtctgagagttgaggacacggccttgtattattgt gcaaaggctacaactgcctggtattcaggcggtctggacgtc IGA1 25 213 14 11 7 14 7 11 3 2 12 8 JY8QFUQ01C3JTT 12466 productive (see comment) Homsap IGHV3-7*02 F 917 92.09 198/215 nt 91.16 196/215 nt Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD5-12*01 F 1 7 8 9 7.8.9 [1.16.38.11] CARDQVTEDYW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 29 of V-REGION: 1 nucleotides (from position 11 in the user submitted sequence), (cause frameshift)in FR2-IMGT, from codon 40 of V-REGION: 1 nucleotides (from position 33 in the user submitted sequence), (cause frameshift) ctctggattccttttagaacctattggatgagttggtccgccaggctccagggaaagggctggagtgggtggcccacataaaccaagatggacgtgaggcatactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgtgccagagatcaagtgactgaagactactggggccagggaaccctggtcagtgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaaactc NA IGG1 100 100 [274] c87>t|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|g122>t,W41>L(+ - -)|a163>c,N55>H(- - -)|g171>c,K57>N(+ - -)|a187>c,S63>R(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)|c248>g,A83>G(- + -)|c255>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|g297>c|g301>a,V101>M(+ - -)|c309>t|g315>c| c87>t|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)| g122>t,W41>L(+ - -)|a163>c,N55>H(- - -)| g171>c,K57>N(+ - -)|a187>c,S63>R(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)| c248>g,A83>G(- + -)|c255>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|g297>c|g301>a,V101>M(+ - -)|c309>t| g315>c| 318 221 203 18 6 12 1 2 4 0 3 0 0 1 4 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 20 3 1 2 0 0 1 0 0 0 0 1 1 0 0 0 51 50 48 2 0 2 0 0 0 0 1 0 0 0 0 0 1 0 30 24 20 4 0 4 1 0 0 0 2 0 0 0 1 0 0 0 117 114 106 8 4 4 0 2 3 0 0 0 0 0 1 2 0 0 6 6 5 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattccttttagaacctattgg atgagttggtccgccaggctccagggaaagggctggagtgggtggcccac ataaaccaagatggacgtgaggca tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac ARDQVTEDY IGHV3-7 IGHD5-12 IGHJ4 ggattccttttagaacctattgg atgagttggtccgccaggctccagggaaagggctggagtgggtggcccac ataaaccaagatggacgtgaggca tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac IGG1 17 211 7 10 6 12 1 5 4 1 6 6 JY8QFUQ01C3KAU 16188 productive (see comment) Homsap IGHV3-48*01 F, or Homsap IGHV3-48*02 F or Homsap IGHV3-48*04 F 874 89.04 195/219 nt 88.13 193/219 nt Homsap IGHJ6*02 F (see comment) 188 78.69 48/61 nt Homsap IGHD3-16*01 F 3 8 8 12 8.8.12 [2.17.38.11] CAREGGRGYDMDVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 54 of V-REGION: 'C' (1 nucleotides at position 78 to 78 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 59 of V-REGION: 'G' (1 nucleotides at position 93 to 93 in the user submitted sequence), (cause frameshift); gcctctggattcaccttcagtatctatgccatgacctgggtccgccaggctccagggaaggggctggagtggatttcCatttatcactgataGggggtagtacccaatactacgcagactctgtgaagggccgattcaccgtctccagggaccaagccaagaactcactgtatctacaaatgaacaacctgggagtcgaggacacggctgtgtattattgtgcgagagaggggggacgtggctacgatatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [288] g107>t,S36>I(- - -)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)|a119>c,N40>T(- + -)|g157>a,V53>I(+ - +)|a164>t,Y55>F(- + -)|c165>t,Y55>F(- + -)|t168>c|g170>c,S57>T(+ - +)|a172>g,S58>D(- - -)|g173>a,S58>D(- - -)|t177>g,S59>R(- - -)|a184>g,S62>G(+ + -)|a193>c,I65>Q(- - -)|t194>a,I65>Q(- - -)|a232>g,I78>V(+ - +)|a240>g|a244>c,N82>Q(+ - +)|t246>a,N82>Q(+ - +)|g267>a|g278>a,S93>N(- - -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|c309>t| g107>t,S36>I(- - -)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)| a119>c,N40>T(- + -)|g157>a,V53>I(+ - +)|a164>t,Y55>F(- + -)|c165>t,Y55>F(- + -)| t168>c|g170>c,S57>T(+ - +)|a172>g,S58>D(- - -)|g173>a,S58>D(- - -)|t177>g,S59>R(- - -)|a184>g,S62>G(+ + -)|a193>c,I65>Q(- - -)|t194>a,I65>Q(- - -)| a232>g,I78>V(+ - +)|a240>g|a244>c,N82>Q(+ - +)|t246>a,N82>Q(+ - +)|g267>a|g278>a,S93>N(- - -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|c309>t| 320 227 203 24 4 20 6 4 3 1 3 0 1 2 2 0 1 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 0 0 0 0 0 0 0 1 0 1 0 51 51 47 4 0 4 0 1 1 0 1 0 1 0 0 0 0 0 30 24 16 8 1 7 2 1 0 1 1 0 0 1 1 0 0 1 117 114 105 9 3 6 3 2 2 0 1 0 0 1 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcaccttcagtatctatgcc atgacctgggtccgccaggctccagggaaggggctggagtggatttcattt atcactgataggggtagtacccaa tactacgcagactctgtgaagggccgattcaccgtctccagggaccaagccaagaactcactgtatctacaaatgaacaacctgggagtcgaggacacggctgtgtattattgt gcgagagaggggggacgtggctacgatatggacgtc AREGGRGYDMDV IGHV3-48 IGHD3-16 IGHJ6 ggattcaccttcagtatctatgcc atgacctgggtccgccaggctccagggaaggggctggagtggatttcattt atcactgataggggtagtacccaa tactacgcagactctgtgaagggccgattcaccgtctccagggaccaagccaagaactcactgtatctacaaatgaacaacctgggagtcgaggacacggctgtgtattattgt gcgagagaggggggacgtggctacgatatggacgtc IGA2 24 213 14 10 7 10 7 14 3 1 10 10 JY8QFUQ01C4D0M 17050 productive Homsap IGHV3-66*01 F, or Homsap IGHV3-66*02 F or Homsap IGHV3-66*04 F 926 92.56 199/215 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 2 8 7 15 8.7.15 [1.17.38.11] CARDMGSGRYFEPYDYW in-frame + cctctggattcaccgtcagtagcagcttcatgacttgggtccgccaggctccaggaaagggactggagtgggtctcagtgctttatgtcggtggtaacacatactacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatttgcaactgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagatatgggttcgggaagatattttgaaccttatgactactggggccagggaaccccggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcacccttctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcag NA IGG1 79 88 [292] a110>g,N37>S(- - -)|a113>t,Y38>F(- + -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g141>a|g147>a|t165>g|a166>c,I56>L(+ + +)|a172>g,S58>V(- - -)|g173>t,S58>V(- - -)|g191>a,S64>N(- - -)|a223>c|c259>g,L87>V(+ - +)|c265>t|t267>g|a271>c,M91>L(+ + -)| a110>g,N37>S(- - -)|a113>t,Y38>F(- + -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g141>a|g147>a|t165>g| a166>c,I56>L(+ + +)|a172>g,S58>V(- - -)|g173>t,S58>V(- - -)|g191>a,S64>N(- - -)| a223>c|c259>g,L87>V(+ - +)|c265>t|t267>g|a271>c,M91>L(+ + -)| 320 223 207 16 6 10 2 3 2 0 3 0 1 0 1 1 1 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 51 51 46 5 3 2 0 2 1 0 0 0 0 0 1 0 0 1 30 21 17 4 0 4 1 1 0 0 1 0 0 0 0 0 1 0 117 114 109 5 3 2 0 0 1 0 2 0 0 0 0 1 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|ggca,220-223(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccgtcagtagcagcttc atgacttgggtccgccaggctccaggaaagggactggagtgggtctcagtg ctttatgtcggtggtaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatttgcaactgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagatatgggttcgggaagatattttgaaccttatgactac ARDMGSGRYFEPYDY IGHV3-66 IGHD3-10 IGHJ4 ggattcaccgtcagtagcagcttc atgacttgggtccgccaggctccaggaaagggactggagtgggtctcagtg ctttatgtcggtggtaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatttgcaactgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagatatgggttcgggaagatattttgaaccttatgactac IGG1 16 210 7 9 5 8 2 8 6 0 4 6 JY8QFUQ01C50HS 11701 productive Homsap IGHV4-61*02 F (see comment) 569 73.3 162/221 nt NA Homsap IGHJ4*02 F (see comment) 141 77.08 37/48 nt Homsap IGHD6-6*01 F 1 10 7 16 10.7.16 [1.17.38.11] CARECSNSKVGPRALDSW in-frame + Low V-REGION identity (73,30% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ5*01 (highest number of consecutive identical nucleotides) tttctggtggcccgatcaaaagtcctgattaccattggacgtggatccggcaggccgccgggaaggggctggagtgggtcgggcgtgtctatatgactggctatgtcgagaacaatccatccctctccgggcgtctctccatgtcgattgacacggcgaagaatcagttttctatgacattgacttctgtgaccgccgcagacacggccctttatttttgtgcgagggagtgtagtaattcgaaagtcggaccgagagcgcttgactcctggggcccgggaagtctggtcaccgtatcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [300] c75>t|t85>c,S29>P(+ - -)|c87>g,S29>P(+ - -)|g92>a,S31>K(- - -)|c93>a,S31>K(- - -)|g103>c,G35>P(+ - -)|g104>c,G35>P(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t112>c,Y38>H(+ - -)|c114>t,Y38>H(+ - -)|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>g,P45>A(- - -)|a147>g|a157>g,I53>V(+ - +)|t159>c,I53>V(+ - +)|a166>g,I56>V(+ - +)|c173>t,T58>M(- - -)|c174>g,T58>M(- - -)|g176>c,S59>T(+ - +)|g189>c|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|c192>t,S64>Y(+ - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|a196>g,N66>E(+ - -)|c198>g,N66>E(+ - -)|t199>a,Y67>N(- - -)|c204>t|c207>a|a214>t,K72>S(- - -)|a215>c,K72>S(- - -)|g216>c,K72>S(- - -)|a220>g,S74>G(+ + -)|t222>g,S74>G(+ + -)|a225>t|g226>c,V76>L(+ - +)|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|a237>g|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|t247>g,S83>A(- + -)|c249>g,S83>A(- + -)|c255>t|c261>t|c264>t|c265>a,L89>M(+ + -)|a269>c,K90>T(- - -)|g270>a,K90>T(- - -)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)|a318>g| c75>t| t85>c,S29>P(+ - -)|c87>g,S29>P(+ - -)|g92>a,S31>K(- - -)|c93>a,S31>K(- - -)|g103>c,G35>P(+ - -)|g104>c,G35>P(+ - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t112>c,Y38>H(+ - -)|c114>t,Y38>H(+ - -)| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>g,P45>A(- - -)|a147>g|a157>g,I53>V(+ - +)|t159>c,I53>V(+ - +)| a166>g,I56>V(+ - +)|c173>t,T58>M(- - -)|c174>g,T58>M(- - -)|g176>c,S59>T(+ - +)|g189>c|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|c192>t,S64>Y(+ - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)| a196>g,N66>E(+ - -)|c198>g,N66>E(+ - -)|t199>a,Y67>N(- - -)|c204>t|c207>a|a214>t,K72>S(- - -)|a215>c,K72>S(- - -)|g216>c,K72>S(- - -)|a220>g,S74>G(+ + -)|t222>g,S74>G(+ + -)|a225>t|g226>c,V76>L(+ - +)|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|a237>g|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|t247>g,S83>A(- + -)|c249>g,S83>A(- + -)|c255>t|c261>t|c264>t|c265>a,L89>M(+ + -)|a269>c,K90>T(- - -)|g270>a,K90>T(- - -)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g301>c,V101>L(+ - +)|g303>t,V101>L(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| a318>g| 320 229 169 60 12 48 10 5 12 3 2 3 6 1 9 6 1 2 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 30 20 10 0 10 1 2 1 2 0 1 0 0 2 1 0 0 51 51 45 6 1 5 2 0 0 1 0 0 0 0 1 2 0 0 30 21 11 10 1 9 2 1 3 0 0 0 1 0 2 1 0 0 117 114 82 32 8 24 4 2 7 0 2 2 5 1 4 2 1 2 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tttct ggtggcccgatcaaaagtcctgattaccat tggacgtggatccggcaggccgccgggaaggggctggagtgggtcgggcgt gtctatatgactggctatgtc gagaacaatccatccctctccgggcgtctctccatgtcgattgacacggcgaagaatcagttttctatgacattgacttctgtgaccgccgcagacacggccctttatttttgt gcgagggagtgtagtaattcgaaagtcggaccgagagcgcttgactcc ARECSNSKVGPRALDS IGHV4-61 IGHD6-6 IGHJ4 ggtggcccgatcaaaagtcctgattaccat tggacgtggatccggcaggccgccgggaaggggctggagtgggtcgggcgt gtctatatgactggctatgtc gagaacaatccatccctctccgggcgtctctccatgtcgattgacacggcgaagaatcagttttctatgacattgacttctgtgaccgccgcagacacggccctttatttttgt gcgagggagtgtagtaattcgaaagtcggaccgagagcgcttgactcc IGA1 58 216 28 30 16 35 12 23 9 1 29 19 JY8QFUQ01C5CP6 14222 productive (see comment) Homsap IGHV3-7*02 F 1004 95.87 209/218 nt 95.41 208/218 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSGYYSTGYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 61 to 61 in the user submitted sequence), (cause frameshift); cctctggattcacctttagtacctattggatgagctgggtccgccaggctccagggaaagGggctggagtgggtggccaacataaagcatgatgcaagtgagaaatactatgtggactctgtaaaaggccgattcaccatctccagagacaacgccaagaactcattgtatttacaaatgaacaacctgagagccgaggacacggctgtgtattactgtgctattagtagtggttattactcgacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [283] g107>c,S36>T(+ - +)|a174>t,Q58>H(- + -)|g185>c,G62>A(- + -)|g213>a|g216>a|c259>t|c265>t|g267>a|g278>a,S93>N(- - -)| g107>c,S36>T(+ - +)| a174>t,Q58>H(- + -)|g185>c,G62>A(- + -)| g213>a|g216>a|c259>t|c265>t|g267>a|g278>a,S93>N(- - -)| g315>t|g317>t,R106>I(- + -)|a318>t,R106>I(- + -)| 314 (318) 220 (224) 211 (212) 9 (12) 5 (6) 4 (6) 0 4 2 0 0 0 1 (2) 0 2 0 0 (2) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 1 0 1 0 0 0 117 114 108 6 5 1 0 4 2 0 0 0 0 0 0 0 0 0 2 (6) 2 2 (3) 0 (3) 0 (1) 0 (2) 0 0 0 0 0 0 0 (1) 0 0 0 0 (2) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagtacctattgg atgagctgggtccgccaggctccagggaaagggctggagtgggtggccaac ataaagcatgatgcaagtgagaaa tactatgtggactctgtaaaaggccgattcaccatctccagagacaacgccaagaactcattgtatttacaaatgaacaacctgagagccgaggacacggctgtgtattactgt gctattagtagtggttattactcgacgggctac AISSGYYSTGY IGHV3-7 IGHD3-22 IGHJ4 ggattcacctttagtacctattgg atgagctgggtccgccaggctccagggaaagggctggagtgggtggccaac ataaagcatgatgcaagtgagaaa tactatgtggactctgtaaaaggccgattcaccatctccagagacaacgccaagaactcattgtatttacaaatgaacaacctgagagccgaggacacggctgtgtattactgt gctattagtagtggttattactcgacgggctac IGA2 9 213 6 3 6 8 0 1 5 0 1 3 JY8QFUQ01C5MCI 19016 productive Homsap IGHV3-20*01 F, or Homsap IGHV3-20*02 ORF 891 90.32 196/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVRAGFGELSGFDCW in-frame - ctctggatcacctttggttattgatggcatgagctgggtccgccaagctccggggagggggctggagtgggtctctggtattaatcggaatggtgatagcacaggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgtgtgagagcagggttcggggagttatcgggctttgactgctggggccagggaaccctggtcaccgtgtcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [288] t83>c,F28>S(- - -)|c84>a,F28>S(- - -)|a85>c,T29>P(+ + -)|c87>t,T29>P(+ + -)|t90>g,F30>L(+ - -)|a104>t,D35>V(- - -)|g106>a,D36>I(- - -)|a107>t,D36>I(- - -)|t109>g,Y37>D(- - -)|a138>g|a143>g,K48>R(+ + +)|t172>c,W58>R(- - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a|c314>t,A105>V(+ - +)| t83>c,F28>S(- - -)|c84>a,F28>S(- - -)|a85>c,T29>P(+ + -)|c87>t,T29>P(+ + -)|t90>g,F30>L(+ - -)|a104>t,D35>V(- - -)|g106>a,D36>I(- - -)|a107>t,D36>I(- - -)|t109>g,Y37>D(- - -)| a138>g|a143>g,K48>R(+ + +)| t172>c,W58>R(- - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|g221>a,G74>D(- - -)|g267>c|c268>g,Q90>E(+ + -)|a274>t,N92>F(- - -)|a275>t,N92>F(- - -)|g284>c,R95>T(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>c| 319 (320) 224 (225) 202 22 (23) 3 (4) 19 2 4 2 2 2 (3) 1 4 0 2 1 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 15 9 0 9 0 1 1 1 1 1 2 0 0 0 0 2 51 51 49 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 117 114 106 8 2 6 0 2 0 0 1 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)| ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|tt,198-199(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)| agct,8-11(FR1)|ggta,36-39(FR1)|ggca,112-115(CDR1)|agct,118-121(FR2)|agct,132-135(FR2)|ggct,146-149(FR2)|ggta,163-166(FR2-CDR2)|ggta,187-190(CDR2)|ggtt,196-199(FR3)| agct,8-11(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|agct,132-135(FR2)|aact,253-256(FR3)|agcc,285-288(FR3)| ctct ggatcacctttggttattgatggc atgagctgggtccgccaagctccggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc VRAGFGELSGFDC IGHV3-20 IGHD3-10 IGHJ4 ggatcacctttggttattgatggc atgagctgggtccgccaagctccggggagggggctggagtgggtctctggt attaatcggaatggtgatagcaca ggttatgcagactctgtgcaggaccgattcaccatctccagagacaacgccaagaactccctgtatctcgaaatgttcagtctgacagccgaggacacggccttatatcactgt gtgagagcagggttcggggagttatcgggctttgactgc IGG1 21 213 9 12 5 9 4 12 3 0 7 11 JY8QFUQ01C6DDZ 5075 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 918 91.71 199/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD5-12*01 F 3 8 8 13 8.8.13 [1.17.38.11] CAKDMGGDYYIFHYW in-frame + ttctggattctcctttagcaactatgccctggcctgggtccgccaggctccagggaaggggctggagtgggtctcaattgttagtggaagtggtactggcacaggccacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaaggatatgggtggcgactattacatctttcactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] c75>t|a85>t,T29>S(+ - +)|g107>a,S36>N(- - -)|a115>c,M39>L(+ + -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g163>a,A55>I(+ - +)|c164>t,A55>I(+ - +)|a166>g,I56>V(+ - +)|t174>a|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|a190>g,S64>G(+ + -)|t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|g261>a|a318>g| c75>t| a85>t,T29>S(+ - +)|g107>a,S36>N(- - -)| a115>c,M39>L(+ + -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g163>a,A55>I(+ - +)|c164>t,A55>I(+ - +)| a166>g,I56>V(+ - +)|t174>a|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|a190>g,S64>G(+ + -)| t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|g261>a| a318>g| 320 225 206 19 4 15 6 4 2 1 1 0 1 1 2 0 0 1 78 4 3 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 1 0 0 0 0 0 51 51 46 5 0 5 1 1 1 0 1 0 0 0 1 0 0 0 30 24 19 5 1 4 2 1 0 0 0 0 0 1 1 0 0 0 117 114 109 5 1 4 2 1 0 1 0 0 0 0 0 0 0 1 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ttct ggattctcctttagcaactatgcc ctggcctgggtccgccaggctccagggaaggggctggagtgggtctcaatt gttagtggaagtggtactggcaca ggccacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaggatatgggtggcgactattacatctttcactac AKDMGGDYYIFHY IGHV3-23 IGHD5-12 IGHJ4 ggattctcctttagcaactatgcc ctggcctgggtccgccaggctccagggaaggggctggagtgggtctcaatt gttagtggaagtggtactggcaca ggccacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaggatatgggtggcgactattacatctttcactac IGA1 17 213 11 6 5 7 6 10 1 1 9 6 JY8QFUQ01C6GOS 15555 productive (see comment) Homsap IGHV4-39*03 F 924 91.32 200/219 nt 90.87 199/219 nt Homsap IGHJ3*01 F 232 96 48/50 nt Homsap IGHD3-22*01 F 3 10 7 17 10.7.17 [1.17.38.11] CARSITLTVVVMNDAFDVW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'A' (1 nucleotides at position 27 to 27 in the user submitted sequence), (cause frameshift); ctccggtggctccatcaacagtagaaAattattattggggctggatccgccagcccccagggaagggtttggagtggattggaaatatctattatagtgggaacacctactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgtgcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtctggggccaagggacattggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgaccggtgtcgtggaactccag NA IGG2 100 100 [304] t78>c|g92>a,S31>N(- - -)|t105>a,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t|c114>t|g147>t|c148>t|g162>a|g164>a,S55>N(- - -)|g191>a,S64>N(- - -)|c204>t|a232>g,I78>V(+ - +)|c245>g,T82>R(- - -)|c249>t|c261>g,F87>L(+ - -)|g275>c,S92>T(+ - +)|g280>c,V94>L(+ - +)|g303>a| t78>c| g92>a,S31>N(- - -)|t105>a,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t|c114>t| g147>t|c148>t|g162>a|g164>a,S55>N(- - -)| g191>a,S64>N(- - -)| c204>t|a232>g,I78>V(+ - +)|c245>g,T82>R(- - -)|c249>t|c261>g,F87>L(+ - -)|g275>c,S92>T(+ - +)|g280>c,V94>L(+ - +)|g303>a| 311 219 200 19 9 10 1 6 5 1 0 0 0 1 2 2 1 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 30 25 5 2 3 0 2 2 0 0 0 0 1 0 0 0 0 51 51 47 4 3 1 0 2 1 0 0 0 0 0 0 0 1 0 30 21 20 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 116 114 105 8 3 5 1 1 2 0 0 0 0 0 2 2 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctcc ggtggctccatcaacagtagaaattattat tggggctggatccgccagcccccagggaagggtttggagtggattggaaat atctattatagtgggaacacc tactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgt gcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtc ARSITLTVVVMNDAFDV IGHV4-39 IGHD3-22 IGHJ3 ggtggctccatcaacagtagaaattattat tggggctggatccgccagcccccagggaagggtttggagtggattggaaat atctattatagtgggaacacc tactacaatccgtccctcaagagtcgagtcaccgtatccgtagacaggtctaagaaccagttgtccctgaagctgacctctctgaccgccgcagacacggccgtatattactgt gcgaggagtattactctgacagtagtggttatgaatgatgcttttgatgtc IGG2 18 216 12 6 11 16 1 2 6 2 6 4 JY8QFUQ01C71OC 10124 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 896 90.37 197/218 nt 89.91 196/218 nt Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD5-24*01 ORF 3 8 8 16 8.8.16 [1.17.38.11] CVRYDYSRLHRSNYFGPW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'T' (1 nucleotides at position 49 to 49 in the user submitted sequence), (cause frameshift); tctctggattcaccttcatcagttatggcatgagttgggtccgccaggTttccagggaaggggctggagtgggtctcatctattagtgattatggtaataccgcattctacgcagactccgtgaagggccggttcaccatctccagagacaattccaacaacacgctgtttctgcaaatgagcagcctgagagccgaggacacggccgtttattattgtgtgagatatgactactcacgcctacacagatccaactacttcggcccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [298] c74>t|t90>c|g104>t,S35>I(- - -)|c108>t|c113>g,A38>G(- + -)|c120>t|c134>t,A45>V(+ - +)|g163>t,A55>S(- + -)|g173>a,G58>D(- - -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|g187>a,G63>N(- - -)|g188>a,G63>N(- - -)|g191>c,S64>T(+ - +)|a193>g,T65>A(- - -)|a197>t,Y66>F(- + -)|g252>c,K84>N(+ - -)|a263>t,Y88>F(- + -)|a275>g,N92>S(- - -)|a303>t|c309>t|c314>t,A105>V(+ - +)| c74>t| t90>c|g104>t,S35>I(- - -)|c108>t|c113>g,A38>G(- + -)| c120>t|c134>t,A45>V(+ - +)|g163>t,A55>S(- + -)| g173>a,G58>D(- - -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|g187>a,G63>N(- - -)|g188>a,G63>N(- - -)|g191>c,S64>T(+ - +)|a193>g,T65>A(- - -)| a197>t,Y66>F(- + -)|g252>c,K84>N(+ - -)|a263>t,Y88>F(- + -)|a275>g,N92>S(- - -)|a303>t|c309>t| c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)|g319>t| 316 (320) 222 (226) 200 (202) 22 (24) 6 (7) 16 (17) 2 (3) 4 6 1 0 0 4 0 2 1 2 (3) 0 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 0 1 1 0 0 0 0 0 1 1 0 51 51 48 3 1 2 0 0 2 0 0 0 0 0 0 0 1 0 30 24 17 7 0 7 1 4 0 0 0 0 1 0 1 0 0 0 117 114 108 6 2 4 1 0 1 0 0 0 3 0 1 0 0 0 4 (8) 4 3 (5) 1 (3) 0 (1) 1 (2) 0 (1) 0 1 0 0 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tctct ggattcaccttcatcagttatggc atgagttgggtccgccaggttccagggaaggggctggagtgggtctcatct attagtgattatggtaataccgca ttctacgcagactccgtgaagggccggttcaccatctccagagacaattccaacaacacgctgtttctgcaaatgagcagcctgagagccgaggacacggccgtttattattgt gtgagatatgactactcacgcctacacagatccaactacttcggcccc VRYDYSRLHRSNYFGP IGHV3-23 IGHD5-24 IGHJ5 ggattcaccttcatcagttatggc atgagttgggtccgccaggttccagggaaggggctggagtgggtctcatct attagtgattatggtaataccgca ttctacgcagactccgtgaagggccggttcaccatctccagagacaattccaacaacacgctgtttctgcaaatgagcagcctgagagccgaggacacggccgtttattattgt gtgagatatgactactcacgcctacacagatccaactacttcggcccc IGA2 20 213 11 9 8 13 3 7 3 2 6 9 JY8QFUQ01C73TY 14144 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 956 92.76 205/221 nt NA Homsap IGHJ5*02 F (see comment) 147 76.47 39/51 nt Homsap IGHD3-10*01 F 2 10 7 13 10.7.13 [1.17.38.11] CARRGSYGSGSYYPW in-frame + Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) tctctggtggcgccatcagcagtaatagttactactgggactggatccgccagcccccagggaaggggctggagtggattgggagtatgttttatactggggtcaccttctacaacccgtccctcaagagtcgagttaacatttccgtggacacgtccaagagccagttctccctgaggctgagctctgtgaccgccgcagacacggctgtgtatcactgtgcgagacgtggttcctatggttcggggagttattatccctggggccggggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [291] t85>g,S29>A(- + -)|g104>a,S35>N(- - -)|g119>a,G40>D(- - -)|c168>g,I56>M(+ + -)|a170>t,Y57>F(- + -)|g176>c,S59>T(+ - +)|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|a197>t,Y66>F(- + -)|c228>t|c230>a,T77>N(- + -)|a234>t|a240>g|a254>g,N85>S(- - -)|a269>g,K90>R(+ + +)|t307>c,Y103>H(+ - -)| t85>g,S29>A(- + -)|g104>a,S35>N(- - -)| g119>a,G40>D(- - -)| c168>g,I56>M(+ + -)|a170>t,Y57>F(- + -)|g176>c,S59>T(+ - +)|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)| a197>t,Y66>F(- + -)|c228>t|c230>a,T77>N(- + -)|a234>t|a240>g|a254>g,N85>S(- - -)|a269>g,K90>R(+ + +)|t307>c,Y103>H(+ - -)| a320>g| 319 (320) 228 (229) 212 16 (17) 3 (4) 13 4 (5) 2 1 1 0 1 3 0 1 1 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 21 16 5 0 5 1 0 0 0 0 0 1 0 1 1 1 0 117 114 106 8 3 5 3 0 1 1 0 1 2 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggcgccatcagcagtaatagttactac tgggactggatccgccagcccccagggaaggggctggagtggattgggagt atgttttatactggggtcacc ttctacaacccgtccctcaagagtcgagttaacatttccgtggacacgtccaagagccagttctccctgaggctgagctctgtgaccgccgcagacacggctgtgtatcactgt gcgagacgtggttcctatggttcggggagttattatccc ARRGSYGSGSYYP IGHV4-39 IGHD3-10 IGHJ5 ggtggcgccatcagcagtaatagttactac tgggactggatccgccagcccccagggaaggggctggagtggattgggagt atgttttatactggggtcacc ttctacaacccgtccctcaagagtcgagttaacatttccgtggacacgtccaagagccagttctccctgaggctgagctctgtgaccgccgcagacacggctgtgtatcactgt gcgagacgtggttcctatggttcggggagttattatccc IGA1 16 216 8 8 3 7 5 9 3 0 6 7 JY8QFUQ01C7540 7368 productive Homsap IGHV3-30*01 F 945 93.09 202/217 nt NA Homsap IGHJ4*02 F 240 100 48/48 nt Homsap IGHD3-10*02 F 1 8 8 16 8.8.16 [1.17.38.11] CVKNDLSWGTIEHYFDYW in-frame - ctctggattcatcttcagcaaccttgcgatgcactgggtccgccaggctccaggcaaggggctagagtgggtggcacacatatcatatgatggaaataagaaatactacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgacagctgaagacacggctatttattactgtgtgaaaaatgacctgtcttggggcacaatagagcactactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [296] c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)|t109>c,Y37>L(- - -)|a110>t,Y37>L(- - -)|t114>g|g163>c,V55>H(- + -)|t164>a,V55>H(- + -)|t165>c,V55>H(- + -)|g188>a,S63>N(- - -)|t192>g,N64>K(+ - -)|g284>c,R95>T(- - -)|g291>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|c314>t,A105>V(+ - +)| c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)|t109>c,Y37>L(- - -)|a110>t,Y37>L(- - -)|t114>g| g163>c,V55>H(- + -)|t164>a,V55>H(- + -)|t165>c,V55>H(- + -)| g188>a,S63>N(- - -)|t192>g,N64>K(+ - -)| g284>c,R95>T(- - -)|g291>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| c314>t,A105>V(+ - +)|g317>a,R106>K(+ + +)|g319>a| 316 (320) 221 (225) 205 (207) 16 (18) 3 (4) 13 (14) 0 4 (6) 2 3 0 0 1 1 2 0 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 2 4 0 1 1 2 0 0 1 0 0 0 0 1 51 51 48 3 0 3 0 0 0 1 0 0 0 1 1 0 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 117 114 110 4 1 3 0 2 0 0 0 0 0 0 1 0 1 0 4 (8) 4 3 (5) 1 (3) 0 (1) 1 (2) 0 0 (2) 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagcaaccttgcg atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcacac atatcatatgatggaaataagaaa tactacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgacagctgaagacacggctatttattactgt gtgaaaaatgacctgtcttggggcacaatagagcactactttgactac VKNDLSWGTIEHYFDY IGHV3-30 IGHD3-10 IGHJ4 ggattcatcttcagcaaccttgcg atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcacac atatcatatgatggaaataagaaa tactacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgacagctgaagacacggctatttattactgt gtgaaaaatgacctgtcttggggcacaatagagcactactttgactac IGA1 15 213 8 7 5 8 3 7 1 2 6 6 JY8QFUQ01C7QU9 11803 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 976 94.91 205/216 nt NA Homsap IGHJ5*02 F 246 98.04 50/51 nt Homsap IGHD2-8*01 F 2 8 8 16 8.8.16 [1.17.38.11] CARQSVPYTEYFNWFDPW in-frame - tctggattcaccttcagtacctatggcatgcactgggtccgccaggctccaggcagggggctggagtgggtggcaattatatggcatgatggaaccaataaatactatgcagactccgtgaagggccgattcaccgtctccagagacaattccaagaacacagtgtatctgcaaatgaatagcctgagagccgaggacacggctgtgtattattgtgcgagacagtctgtaccatataccgaatatttcaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [295] g107>c,S36>T(+ - +)|a143>g,K48>R(+ + +)|g163>a,V55>I(+ - +)|t172>c,Y58>H(+ - -)|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)|a232>g,I78>V(+ - +)|g258>a|c259>g,L87>V(+ - +)|c276>t|c309>t| g107>c,S36>T(+ - +)| a143>g,K48>R(+ + +)|g163>a,V55>I(+ - +)| t172>c,Y58>H(+ - -)|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)| a232>g,I78>V(+ - +)|g258>a|c259>g,L87>V(+ - +)|c276>t|c309>t| g319>c| 318 (320) 222 (224) 211 (212) 11 (12) 3 (4) 8 2 2 2 2 0 0 0 0 2 (3) 1 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 49 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 0 0 2 0 0 0 0 1 0 0 0 117 114 109 5 3 2 1 1 2 0 0 0 0 0 0 1 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcagtacctatggc atgcactgggtccgccaggctccaggcagggggctggagtgggtggcaatt atatggcatgatggaaccaataaa tactatgcagactccgtgaagggccgattcaccgtctccagagacaattccaagaacacagtgtatctgcaaatgaatagcctgagagccgaggacacggctgtgtattattgt gcgagacagtctgtaccatataccgaatatttcaactggttcgacccc ARQSVPYTEYFNWFDP IGHV3-33 IGHD2-8 IGHJ5 ggattcaccttcagtacctatggc atgcactgggtccgccaggctccaggcagggggctggagtgggtggcaatt atatggcatgatggaaccaataaa tactatgcagactccgtgaagggccgattcaccgtctccagagacaattccaagaacacagtgtatctgcaaatgaatagcctgagagccgaggacacggctgtgtattattgt gcgagacagtctgtaccatataccgaatatttcaactggttcgacccc IGA1 11 213 8 3 4 7 4 4 3 0 4 4 JY8QFUQ01C7ZZV 6593 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 909 91.24 198/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD1-14*01 ORF 3 8 8 9 8.8.9 [1.17.38.11] CAKEEVPNDYW in-frame + ctctggattcacctttagcaatgctgccatgacgtgggtccgccaggctccagggaaggggctagagtgggtctcaggtattagtattagtggtgatagaacatattacgcagactccgtgaagggccggttcaccatctctagagacaattccaagaataccgtgtatctgcaaatgaacagcctgagagccgaggacacggccatatattattgtgcgaaagaggaggtcccgaatgattattggggccagggaactctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtccgtcgcatgcctggtccagggcttcttcccccag NA IGA2 93 95 [275] g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>g,Y37>A(- - -)|a110>c,Y37>A(- - -)|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|g150>a|c164>g,A55>G(- + -)|g172>a,G58>I(- - -)|g173>t,G58>I(- - -)|g188>a,G63>D(- - -)|c192>a,S64>R(- - -)|c198>t|c237>t|c255>t|g258>c|c259>g,L87>V(+ - +)|g301>a,V101>I(+ - +)|c309>t| g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>g,Y37>A(- - -)|a110>c,Y37>A(- - -)| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|g150>a|c164>g,A55>G(- + -)| g172>a,G58>I(- - -)|g173>t,G58>I(- - -)|g188>a,G63>D(- - -)|c192>a,S64>R(- - -)| c198>t|c237>t|c255>t|g258>c|c259>g,L87>V(+ - +)|g301>a,V101>I(+ - +)|c309>t| 320 225 206 19 6 13 0 5 5 0 1 1 0 0 2 3 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 1 0 1 0 0 0 0 0 0 1 51 51 47 4 1 3 0 1 0 0 0 0 0 0 1 2 0 0 30 24 20 4 0 4 0 2 0 0 0 1 0 0 0 0 1 0 117 114 107 7 5 2 0 1 4 0 0 0 0 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcaatgctgcc atgacgtgggtccgccaggctccagggaaggggctagagtgggtctcaggt attagtattagtggtgatagaaca tattacgcagactccgtgaagggccggttcaccatctctagagacaattccaagaataccgtgtatctgcaaatgaacagcctgagagccgaggacacggccatatattattgt gcgaaagaggaggtcccgaatgattat AKEEVPNDY IGHV3-23 IGHD1-14 IGHJ4 ggattcacctttagcaatgctgcc atgacgtgggtccgccaggctccagggaaggggctagagtgggtctcaggt attagtattagtggtgatagaaca tattacgcagactccgtgaagggccggttcaccatctctagagacaattccaagaataccgtgtatctgcaaatgaacagcctgagagccgaggacacggccatatattattgt gcgaaagaggaggtcccgaatgattat IGA2 19 213 10 9 10 17 0 2 6 0 5 8 JY8QFUQ01C819B 13699 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD3-10*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKKKHYNSGTYLYNFDYW in-frame + ctctggattcacctttagcaactttgccatgacctgggtccgccaggctccagggaggggactggagtgggtctcaactattagtggtagtgatggtagcacatacttcgcagactccgtgaagggccgattcaccatctccagggacaatttcaagaacacgctgtatctgcaaatggacagcctgagagccgaggacacggccgtatattactgtgcgaaaaaaaagcattataattcggggacttatttatacaactttgactactggggccggggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [299] g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)|g119>c,S40>T(+ - +)|a143>g,K48>R(+ + +)|g147>a|g163>a,A55>T(- - -)|g185>a,G62>D(- - -)|a200>t,Y67>F(- + -)|g225>a|a240>g|c248>t,S83>F(- - -)|a274>g,N92>D(+ + -)| g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)| g119>c,S40>T(+ - +)|a143>g,K48>R(+ + +)|g147>a|g163>a,A55>T(- - -)| g185>a,G62>D(- - -)| a200>t,Y67>F(- + -)|g225>a|a240>g|c248>t,S83>F(- - -)|a274>g,N92>D(+ + -)| g319>a| 318 (320) 223 (225) 211 (212) 12 (13) 3 (4) 9 3 5 (6) 1 0 0 0 2 0 1 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 1 0 0 0 0 0 51 51 47 4 1 3 1 2 0 0 0 0 0 0 1 0 0 0 30 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 117 114 109 5 2 3 2 1 1 0 0 0 1 0 0 0 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcaactttgcc atgacctgggtccgccaggctccagggaggggactggagtgggtctcaact attagtggtagtgatggtagcaca tacttcgcagactccgtgaagggccgattcaccatctccagggacaatttcaagaacacgctgtatctgcaaatggacagcctgagagccgaggacacggccgtatattactgt gcgaaaaaaaagcattataattcggggacttatttatacaactttgactac AKKKHYNSGTYLYNFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcacctttagcaactttgcc atgacctgggtccgccaggctccagggaggggactggagtgggtctcaact attagtggtagtgatggtagcaca tacttcgcagactccgtgaagggccgattcaccatctccagggacaatttcaagaacacgctgtatctgcaaatggacagcctgagagccgaggacacggccgtatattactgt gcgaaaaaaaagcattataattcggggacttatttatacaactttgactac IGA1 12 213 9 3 6 7 3 5 3 0 6 3 JY8QFUQ01C847F 7284 productive (see comment) Homsap IGHV3-11*01 F 984 95.79 205/214 nt 95.33 204/214 nt Homsap IGHJ4*02 F 240 100 48/48 nt Homsap IGHD1-1*01 F 3 8 8 13 8.8.13 [1.17.37.11] CARVNTWNADYFDYW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 66 of V-REGION: 3 nucleotides (from position 104 in the user submitted sequence), (do not cause frameshift) ctctggattcaccttcagtgactactacatgatctggatccgccaggctccagggaaggggctggagtgggtttcatacattactagtcgtggtactatcatgtacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggccatttattactgtgcgagagtgaacacatggaacgccgactactttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [285] g119>t,S40>I(- - -)|g170>c,S57>T(+ - +)|a175>c,S59>R(- - -)|g188>c,S63>T(+ - +)|c191>t,T64>I(- - -)|a195>g,I65>M(+ + -)|a263>c,Y88>S(+ - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| g119>t,S40>I(- - -)| g170>c,S57>T(+ - +)|a175>c,S59>R(- - -)|g188>c,S63>T(+ - +)|c191>t,T64>I(- - -)|a195>g,I65>M(+ + -)| a263>c,Y88>S(+ - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| a320>t| 319 (320) 221 (222) 212 9 (10) 0 (1) 9 1 1 1 0 2 0 0 (1) 0 2 0 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 30 24 19 5 0 5 1 0 1 0 1 0 0 0 2 0 0 0 117 111 108 3 0 3 0 1 0 0 1 0 0 0 0 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgactactac atgatctggatccgccaggctccagggaaggggctggagtgggtttcatac attactagtcgtggtactatcatg tacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggccatttattactgt gcgagagtgaacacatggaacgccgactactttgactac ARVNTWNADYFDY IGHV3-11 IGHD1-1 IGHJ4 ggattcaccttcagtgactactac atgatctggatccgccaggctccagggaaggggctggagtgggtttcatac attactagtcgtggtactatcatg tacgcagactctgtgaagggccgattcaccatctccagggacaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggccatttattactgt gcgagagtgaacacatggaacgccgactactttgactac IGG1 9 210 3 6 2 6 1 3 0 0 4 5 JY8QFUQ01C8AA1 9548 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 934 93.43 199/213 nt NA Homsap IGHJ6*02 F (see comment) 175 75.81 47/62 nt Homsap IGHD6-13*01 F 2 8 8 14 8.8.14 [X.17.38.11] CARRPAAGSHFALDVW in-frame - Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) or Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ggatacaactttgacaccgattggatcgcctgggtgcgccagatgcccgggaaaggcctggagtggatggggatcatctatcctgctgactctgataccagatacagtccgtccttccaaggccaagtcaccatctcagccgacaagtccatcaacaccgcctacctgcagtggagcggcctgaaggcctcggacaccgccatctattattgtgcgaggcgtccagcagctggtagccacttcgctttggacgtctggggccaggggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [286] g86>a,S29>N(- - -)|a103>g,T35>D(- + -)|c104>a,T35>D(- + -)|g107>c,S36>T(+ - +)|t109>g,Y37>D(- - -)|c111>t,Y37>D(- - -)|g119>c,G40>A(- + -)|g176>c,G59>A(- + -)|c204>t|g225>a|g254>a,S85>N(- - -)|a277>g,S93>G(+ + -)|g303>c,M101>I(+ + -)|c309>t| g86>a,S29>N(- - -)|a103>g,T35>D(- + -)|c104>a,T35>D(- + -)|g107>c,S36>T(+ - +)|t109>g,Y37>D(- - -)|c111>t,Y37>D(- - -)| g119>c,G40>A(- + -)| g176>c,G59>A(- + -)| c204>t|g225>a|g254>a,S85>N(- - -)|a277>g,S93>G(+ + -)|g303>c,M101>I(+ + -)|c309>t| a318>g|a320>g| 317 (320) 218 (221) 204 (205) 14 (16) 3 (5) 11 2 (4) 3 3 0 0 1 0 0 4 0 0 1 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 1 1 0 0 1 0 0 1 0 0 1 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 117 114 108 6 3 3 1 2 2 0 0 0 0 0 1 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ggatacaactttgacaccgattgg atcgcctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctgctgactctgatacc agatacagtccgtccttccaaggccaagtcaccatctcagccgacaagtccatcaacaccgcctacctgcagtggagcggcctgaaggcctcggacaccgccatctattattgt gcgaggcgtccagcagctggtagccacttcgctttggacgtc ARRPAAGSHFALDV IGHV5-51 IGHD6-13 IGHJ6 ggatacaactttgacaccgattgg atcgcctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctgctgactctgatacc agatacagtccgtccttccaaggccaagtcaccatctcagccgacaagtccatcaacaccgcctacctgcagtggagcggcctgaaggcctcggacaccgccatctattattgt gcgaggcgtccagcagctggtagccacttcgctttggacgtc IGA1 14 213 8 6 6 11 2 3 3 0 4 7 JY8QFUQ01C8ASE 4479 productive Homsap IGHV3-48*03 F 792 85.25 185/217 nt NA Homsap IGHJ6*02 F 229 85.48 53/62 nt Homsap IGHD2-21*02 F 2 8 8 14 8.8.14 [1.17.38.11] CVRQTGDYYFFGLDVW in-frame - ctctggattcgccttcaaaagttttaacatgaattgggtccgccaggcttcagggaaggggccggagtggattgcatacattaatggaagagggactaacatctactatgcagactctgtgaagggccggttcaccatctccagagacaacgcccagaacgcagtgcatctgcagatggaccgcctgagagtcgaggacacggccctatattactgtgtgagacaaactggagactactatttcttcggtctggacgtctggggccaaggaaccacggtcaccgtctcttcatcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [290] a85>g,T29>A(- - -)|g104>a,S35>K(- - -)|t105>a,S35>K(- - -)|a110>t,Y37>F(- + -)|g112>a,E38>N(+ - -)|a114>c,E38>N(+ - -)|c120>t|c136>t,P46>S(+ - -)|t149>c,L50>P(- - -)|g157>a,V53>I(+ - +)|t160>g,S54>A(- + -)|g170>a,S57>N(- - -)|a172>g,S58>G(+ + -)|t174>a,S58>G(+ + -)|t177>a,S59>R(- - -)|t186>g|g188>c,S63>T(+ - +)|c191>a,T64>N(- + -)|a195>c|c201>t|a225>g|a250>c,K84>Q(+ - -)|t256>g,S86>A(- + -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)|a270>g|a274>g,N92>D(+ + -)|a277>c,S93>R(- - -)|c287>t,A96>V(+ - +)|t300>c|g301>c,V101>L(+ - +)|t303>a,V101>L(+ - +)|c314>t,A105>V(+ - +)| a85>g,T29>A(- - -)|g104>a,S35>K(- - -)|t105>a,S35>K(- - -)|a110>t,Y37>F(- + -)|g112>a,E38>N(+ - -)|a114>c,E38>N(+ - -)| c120>t|c136>t,P46>S(+ - -)|t149>c,L50>P(- - -)|g157>a,V53>I(+ - +)|t160>g,S54>A(- + -)| g170>a,S57>N(- - -)|a172>g,S58>G(+ + -)|t174>a,S58>G(+ + -)|t177>a,S59>R(- - -)|t186>g|g188>c,S63>T(+ - +)|c191>a,T64>N(- + -)|a195>c| c201>t|a225>g|a250>c,K84>Q(+ - -)|t256>g,S86>A(- + -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)|a270>g|a274>g,N92>D(+ + -)|a277>c,S93>R(- - -)|c287>t,A96>V(+ - +)|t300>c|g301>c,V101>L(+ - +)|t303>a,V101>L(+ - +)| c314>t,A105>V(+ - +)|g319>c| 318 (320) 223 (225) 190 (191) 33 (34) 7 (8) 26 5 4 5 3 4 1 1 4 2 (3) 1 0 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 2 0 0 1 0 1 1 0 0 0 0 51 51 46 5 1 4 0 1 2 1 0 0 0 0 0 0 0 1 30 24 16 8 2 6 1 1 0 0 1 1 0 2 1 0 0 1 117 114 101 13 4 9 3 0 2 2 2 0 0 1 1 1 0 1 6 (8) 6 5 (6) 1 (2) 0 (1) 1 0 0 1 0 0 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,113-114(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcgccttcaaaagttttaac atgaattgggtccgccaggcttcagggaaggggccggagtggattgcatac attaatggaagagggactaacatc tactatgcagactctgtgaagggccggttcaccatctccagagacaacgcccagaacgcagtgcatctgcagatggaccgcctgagagtcgaggacacggccctatattactgt gtgagacaaactggagactactatttcttcggtctggacgtc VRQTGDYYFFGLDV IGHV3-48 IGHD2-21 IGHJ6 ggattcgccttcaaaagttttaac atgaattgggtccgccaggcttcagggaaggggccggagtggattgcatac attaatggaagagggactaacatc tactatgcagactctgtgaagggccggttcaccatctccagagacaacgcccagaacgcagtgcatctgcagatggaccgcctgagagtcgaggacacggccctatattactgt gtgagacaaactggagactactatttcttcggtctggacgtc IGA1 32 213 16 16 8 12 8 20 5 2 13 12 JY8QFUQ01C8FEX 18512 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 981 94.93 206/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-10*01 F 2 8 8 15 8.8.15 [1.17.38.11] CARDSRHSASGSYSDYW in-frame - gtctggattcaccttcaatagccatggcatgcactgggtccgccaggcgccaggcaaggggctggagtgggtggctgctattcggtttgatggaagtaataaatactatgcagactccgtgaagggacgattcaccatctccagagacaattccaagaacacgttgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtactactgtgcgagagattcccggcactctgcctcggggagttattccgactactggggccagggaaccctggtcgccgtttcttcagcctccaccaagggcccatcggtcttcccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [295, 294] g104>a,S35>N(- - -)|t109>c,Y37>H(+ - -)|t135>g|a162>t|t164>c,V55>A(+ - +)|a168>t|t169>c,W57>R(- - -)|a173>t,Y58>F(- + -)|c222>a|c259>t|t306>c| g104>a,S35>N(- - -)|t109>c,Y37>H(+ - -)| t135>g|a162>t|t164>c,V55>A(+ - +)| a168>t|t169>c,W57>R(- - -)|a173>t,Y58>F(- + -)| c222>a|c259>t|t306>c| 320 225 214 11 6 5 0 1 1 4 0 1 3 0 0 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 0 0 1 0 0 1 0 0 0 0 1 30 24 21 3 1 2 0 0 0 1 0 0 2 0 0 0 0 0 117 114 111 3 3 0 0 0 1 1 0 1 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcaccttcaatagccatggc atgcactgggtccgccaggcgccaggcaaggggctggagtgggtggctgct attcggtttgatggaagtaataaa tactatgcagactccgtgaagggacgattcaccatctccagagacaattccaagaacacgttgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtactactgt gcgagagattcccggcactctgcctcggggagttattccgactac ARDSRHSASGSYSDY IGHV3-33 IGHD3-10 IGHJ4 ggattcaccttcaatagccatggc atgcactgggtccgccaggcgccaggcaaggggctggagtgggtggctgct attcggtttgatggaagtaataaa tactatgcagactccgtgaagggacgattcaccatctccagagacaattccaagaacacgttgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtactactgt gcgagagattcccggcactctgcctcggggagttattccgactac IGG1 11 213 6 5 2 3 4 8 5 1 1 4 JY8QFUQ01C8GT7 17868 productive Homsap IGHV3-48*01 F, or Homsap IGHV3-48*02 F 846 88.02 191/217 nt NA Homsap IGHJ6*02 F 229 85.48 53/62 nt Homsap IGHD2-2*01 F 3 8 8 19 8.8.19 [1.17.38.11] CAKDRTLHTPAQMFYYGMDVW in-frame - atctggattcaccttcagttcttatagtgtaaactgggtccgccaggctccagggaagggcctagagtttgtctcatacattgatagtagtggttctaccatatactacgcagactctgtgaagggccgattcaccatctctagagacaatgcccagaactcactgtttctgcaaatgaacaacctgcgagtcgacgacacggccgtatattactgtgcaaaagaccggacacttcacacacccgctcagatgttttactatggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatctccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 89 [305] c75>a|a106>t|g107>c|c108>t|c114>t|a115>g,M39>V(+ - -)|g117>a,M39>V(+ - -)|g147>c|g150>a|g155>t,W52>F(+ + -)|g156>t,W52>F(+ + -)|t159>c|a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a184>g,S62>G(+ + -)|a187>t|g188>c|c237>t|a250>c,K84>Q(+ - -)|a263>t,Y88>F(- + -)|g278>a,S93>N(- - -)|a283>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t300>c|g303>a|g315>a|g317>a,R106>K(+ + +)| c75>a| a106>t|g107>c|c108>t|c114>t| a115>g,M39>V(+ - -)|g117>a,M39>V(+ - -)|g147>c|g150>a|g155>t,W52>F(+ + -)|g156>t,W52>F(+ + -)|t159>c| a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a184>g,S62>G(+ + -)|a187>t|g188>c| c237>t|a250>c,K84>Q(+ - -)|a263>t,Y88>F(- + -)|g278>a,S93>N(- - -)|a283>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t300>c|g303>a| g315>a|g317>a,R106>K(+ + +)| 320 225 197 28 15 13 3 7 4 2 2 1 3 0 4 0 2 0 78 4 3 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 36 24 20 4 4 0 0 0 2 0 0 0 1 0 1 0 0 0 51 51 44 7 3 4 1 2 0 1 0 0 0 0 1 0 2 0 30 24 19 5 2 3 2 1 0 0 0 0 1 0 1 0 0 0 117 114 105 9 4 5 0 2 2 1 2 0 1 0 1 0 0 0 8 8 6 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| atct ggattcaccttcagttcttatagt gtaaactgggtccgccaggctccagggaagggcctagagtttgtctcatac attgatagtagtggttctaccata tactacgcagactctgtgaagggccgattcaccatctctagagacaatgcccagaactcactgtttctgcaaatgaacaacctgcgagtcgacgacacggccgtatattactgt gcaaaagaccggacacttcacacacccgctcagatgttttactatggtatggacgtc AKDRTLHTPAQMFYYGMDV IGHV3-48 IGHD2-2 IGHJ6 ggattcaccttcagttcttatagt gtaaactgggtccgccaggctccagggaagggcctagagtttgtctcatac attgatagtagtggttctaccata tactacgcagactctgtgaagggccgattcaccatctctagagacaatgcccagaactcactgtttctgcaaatgaacaacctgcgagtcgacgacacggccgtatattactgt gcaaaagaccggacacttcacacacccgctcagatgttttactatggtatggacgtc IGA1 25 213 14 11 9 15 5 10 7 6 9 3 JY8QFUQ01C8YUG 1422 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 809 86.51 186/215 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-15*01 F 2 8 7 10 8.7.10 [1.17.38.11] CATLRGGTFDYW in-frame + tctctggtgcctccatcaggagttattattggagttggatccggcagcccccaggaaagggactggagtggattggttatattaattatgttggggacaccgattacaacccctccctcaagagtcgagtctccatgtcagcagccacgtccaagaaccaggtcttcctgcagctgacctctgtgaccgctgcggacaccgcctattatttctgtgcgacactgagagggggtactttcgactactggggccggggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [277] g83>c,G28>A(- + -)|t105>g,S35>R(- - -)|c111>t|c114>t|c120>t|g141>a|g162>t|c168>t|t169>a,Y57>N(- - -)|c174>t|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a196>g,N66>D(+ + -)|c198>t,N66>D(+ + -)|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|t239>c,V80>A(+ - +)|a242>c,D81>A(- - -)|t259>g,F87>V(+ - -)|c263>t,S88>F(- - -)|a268>c,K90>Q(+ - -)|g275>c,S92>T(+ - +)|g297>c|g301>t,V101>Y(- - -)|t302>a,V101>Y(- - -)|g303>t,V101>Y(- - -)|a308>t,Y103>F(- + -)| g83>c,G28>A(- + -)|t105>g,S35>R(- - -)|c111>t|c114>t| c120>t|g141>a|g162>t| c168>t|t169>a,Y57>N(- - -)|c174>t|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a196>g,N66>D(+ + -)|c198>t,N66>D(+ + -)|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|t239>c,V80>A(+ - +)|a242>c,D81>A(- - -)|t259>g,F87>V(+ - -)|c263>t,S88>F(- - -)|a268>c,K90>Q(+ - -)|g275>c,S92>T(+ - +)|g297>c|g301>t,V101>Y(- - -)|t302>a,V101>Y(- - -)|g303>t,V101>Y(- - -)|a308>t,Y103>F(- + -)| g317>c,R106>T(- - -)|g319>c|a320>t| 316 (320) 219 (223) 190 (191) 29 (32) 8 (10) 21 (22) 4 2 7 1 2 0 2 (3) 2 3 (5) 0 4 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 0 2 0 0 0 0 0 1 0 0 1 51 51 48 3 3 0 0 1 1 0 0 0 0 0 0 0 1 0 30 21 14 7 2 5 2 1 2 0 0 0 0 1 0 0 1 0 117 114 99 15 1 14 2 0 2 1 2 0 2 1 2 0 2 1 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 0 0 0 0 0 0 (1) 0 0 (2) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgcctccatcaggagttattat tggagttggatccggcagcccccaggaaagggactggagtggattggttat attaattatgttggggacacc gattacaacccctccctcaagagtcgagtctccatgtcagcagccacgtccaagaaccaggtcttcctgcagctgacctctgtgaccgctgcggacaccgcctattatttctgt gcgacactgagagggggtactttcgactac ATLRGGTFDY IGHV4-59 IGHD2-15 IGHJ4 ggtgcctccatcaggagttattat tggagttggatccggcagcccccaggaaagggactggagtggattggttat attaattatgttggggacacc gattacaacccctccctcaagagtcgagtctccatgtcagcagccacgtccaagaaccaggtcttcctgcagctgacctctgtgaccgctgcggacaccgcctattatttctgt gcgacactgagagggggtactttcgactac IGG2 29 210 14 15 9 16 5 13 4 4 14 7 JY8QFUQ01C9KPR 2732 productive (see comment) Homsap IGHV3-30*10 F (see comment) 706 81.02 175/216 nt 80.56 174/216 nt Homsap IGHJ1*01 F 152 76.92 40/52 nt Homsap IGHD1-1*01 F 1 8 8 16 8.8.16 [1.17.37.11] CSRGTTGTTDDSRLFQHW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (81,02% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR3-IMGT, from codon 84 of V-REGION: 1 nucleotides (from position 157 in the user submitted sequence), (cause frameshift) ctctggattcagcttcggcaattacgccatgcactgggtccgccaggctccaggcaagggcctagagtgggtggctgtcataaataaggctgggaagactaaacattatatagactccgtgaagggccgattcaacgtctccagagacaactccgaaaaacactctatttggagatgaataacgtgggagttgaggacacggctgtatattattgttcgagaggtaccacggggacgacagacgactcgaggttgttccaacactggggccagggcgccctggtcaccgtttcgtcaacatccccgaccagcccccaaaggtcttcccgctgagccttgacagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 71 79 [297, 295] c86>g,T29>S(+ - +)|a103>g,S35>G(+ + -)|t105>c,S35>G(+ + -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t111>c|t114>c|g147>c|a162>t|t165>c|t169>a,S57>N(- - -)|c170>a,S57>N(- - -)|a171>t,S57>N(- - -)|t172>a,Y58>K(- - -)|t174>g,Y58>K(- - -)|a176>c,D59>A(- - -)|a186>g|g188>a,S63>K(- - -)|t189>g,S63>K(- - -)|a191>c,N64>T(- + -)|t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|c203>t,T68>I(- - -)|c230>a,T77>N(- + -)|a232>g,I78>V(+ - +)|t246>c|a250>g|c255>a,N85>K(+ - -)|g258>a|g261>c|c265>t|c268>g,Q90>E(+ + -)|a270>g,Q90>E(+ + -)|c276>t|g278>a,S93>N(- - -)|c280>g,L94>V(+ - +)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g303>a|c309>t|g313>t,A105>S(- + -)| c86>g,T29>S(+ - +)|a103>g,S35>G(+ + -)|t105>c,S35>G(+ + -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t111>c|t114>c| g147>c|a162>t|t165>c| t169>a,S57>N(- - -)|c170>a,S57>N(- - -)|a171>t,S57>N(- - -)|t172>a,Y58>K(- - -)|t174>g,Y58>K(- - -)|a176>c,D59>A(- - -)|a186>g|g188>a,S63>K(- - -)|t189>g,S63>K(- - -)|a191>c,N64>T(- + -)| t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|c203>t,T68>I(- - -)|c230>a,T77>N(- + -)|a232>g,I78>V(+ - +)|t246>c|a250>g|c255>a,N85>K(+ - -)|g258>a|g261>c|c265>t|c268>g,Q90>E(+ + -)|a270>g,Q90>E(+ + -)|c276>t|g278>a,S93>N(- - -)|c280>g,L94>V(+ - +)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g303>a|c309>t| g313>t,A105>S(- + -)|a320>g| 319 (320) 223 (224) 181 42 (43) 15 (16) 27 6 (7) 5 8 6 2 3 2 2 2 3 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 2 5 1 1 1 3 0 0 0 0 0 1 0 0 51 51 48 3 3 0 0 0 0 1 0 0 1 0 1 0 0 0 30 24 14 10 1 9 1 1 0 0 2 1 1 2 0 0 0 2 117 113 92 21 9 12 4 3 7 2 0 2 0 0 1 2 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 0 0 0 0 0 0 0 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagcttcggcaattacgcc atgcactgggtccgccaggctccaggcaagggcctagagtgggtggctgtc ataaataaggctgggaagactaaa cattatatagactccgtgaagggccgattcaacgtctccagagacaactccgaaaaacactctatttggagatgaataacgtgggagttgaggacacggctgtatattattgt tcgagaggtaccacggggacgacagacgactcgaggttgttccaacac SRGTTGTTDDSRLFQH IGHV3-30 IGHD1-1 IGHJ1 ggattcagcttcggcaattacgcc atgcactgggtccgccaggctccaggcaagggcctagagtgggtggctgtc ataaataaggctgggaagactaaa cattatatagactccgtgaagggccgattcaacgtctccagagacaactccgaaaaacactctatttggagatgaataacgtgggagttgaggacacggctgtatattattgt tcgagaggtaccacggggacgacagacgactcgaggttgttccaacac IGA1 41 212 25 16 13 21 12 20 12 3 12 14 JY8QFUQ01C9RTB 9590 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 1001 95.02 210/221 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD1-7*01 F 2 10 7 12 10.7.12 [1.17.38.11] CVRHRRLELLPDYW in-frame + tctctggtgactccatcagcagtaatagtttctactggggctggatccgccagcccccagggaaggggctggagtggattgggaatatctattatagtgggagcacctactacaacccgtccctcgagagccgagtcaccatatccgtagatacatccaggaaccagttctccctgaagctgaggtttgtgaccgccgcagacacggctgtgtattactgtgtgagacatagaagactggaactactcccagactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [288] g83>a,G28>D(- - -)|g104>a,S35>N(- - -)|a110>t,Y37>F(- + -)|g164>a,S55>N(- - -)|a214>g,K72>E(+ - -)|t222>c|c243>t|g246>a|a251>g,K84>R(+ + +)|c276>g,S92>R(- - -)|c278>t,S93>F(- - -)|c314>t,A105>V(+ - +)| g83>a,G28>D(- - -)|g104>a,S35>N(- - -)|a110>t,Y37>F(- + -)| g164>a,S55>N(- - -)| a214>g,K72>E(+ - -)|t222>c|c243>t|g246>a|a251>g,K84>R(+ + +)|c276>g,S92>R(- - -)|c278>t,S93>F(- - -)| c314>t,A105>V(+ - +)| 320 229 217 12 3 9 2 4 3 1 0 0 1 0 0 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 0 2 0 0 0 0 1 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 107 7 3 4 2 1 2 1 0 0 0 0 0 1 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccatcagcagtaatagtttctac tggggctggatccgccagcccccagggaaggggctggagtggattgggaat atctattatagtgggagcacc tactacaacccgtccctcgagagccgagtcaccatatccgtagatacatccaggaaccagttctccctgaagctgaggtttgtgaccgccgcagacacggctgtgtattactgt gtgagacatagaagactggaactactcccagactac VRHRRLELLPDY IGHV4-39 IGHD1-7 IGHJ4 ggtgactccatcagcagtaatagtttctac tggggctggatccgccagcccccagggaaggggctggagtggattgggaat atctattatagtgggagcacc tactacaacccgtccctcgagagccgagtcaccatatccgtagatacatccaggaaccagttctccctgaagctgaggtttgtgaccgccgcagacacggctgtgtattactgt gtgagacatagaagactggaactactcccagactac IGA1 11 216 9 2 6 7 3 4 3 0 5 3 JY8QFUQ01C9UFU 8438 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 999 95.85 208/217 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F 93 64.71 33/51 nt Homsap IGHD4-17*01 F 2 8 8 9 8.8.9 [1.17.38.11] CARDNYGDYWG in-frame + ctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtgttaatggtgatgggggtagcacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgtgcaagagataattacggtgactactgggggccaggggaacccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [275] a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| 320 225 216 9 4 5 4 1 0 0 0 0 0 0 3 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 3 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 4 2 1 1 0 0 0 0 0 0 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactactgg ARDNYGDYW IGHV3-74 IGHD4-17 IGHJ5 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactactgg IGA2 9 213 5 4 1 5 4 4 4 0 2 3 JY8QFUQ01CA1G6 9156 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 1007 96.74 208/215 nt 95.81 206/215 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD3-10*01 F 2 8 7 13 8.7.13 [1.17.38.11] CAREDGSGRRHFDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 44 of V-REGION: 'G' (1 nucleotides at position 47 to 47 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 57 of V-REGION: 'C' (1 nucleotides at position 85 to 85 in the user submitted sequence), (cause frameshift); tctctggtggctccatcagtagttactactggagctggatccggcaGgcccccaggaaagggactggagtggattgggtatatcCttttacactgggaccaccaactacaacccctccctcaagagtcgagtcaccatgtcaatagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtctattactgtgcgagagaggacgggtccggcagacgtcactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcc NA IGA1 100 100 [287] g141>a|a170>t,Y57>F(- + -)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)|a234>g,I78>M(+ + -)|g238>a,V80>I(+ - +)|g303>c| g141>a| a170>t,Y57>F(- + -)|g176>c,S59>T(+ - +)|g191>c,S64>T(+ - +)| a234>g,I78>M(+ + -)|g238>a,V80>I(+ - +)|g303>c| 320 223 216 7 2 5 1 2 0 0 0 0 1 0 3 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 21 18 3 0 3 0 0 0 0 0 0 1 0 2 0 0 0 117 114 111 3 1 2 1 1 0 0 0 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagtagttactac tggagctggatccggcagcccccaggaaagggactggagtggattgggtat atcttttacactgggaccacc aactacaacccctccctcaagagtcgagtcaccatgtcaatagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtctattactgt gcgagagaggacgggtccggcagacgtcactttgactac AREDGSGRRHFDY IGHV4-59 IGHD3-10 IGHJ4 ggtggctccatcagtagttactac tggagctggatccggcagcccccaggaaagggactggagtggattgggtat atcttttacactgggaccacc aactacaacccctccctcaagagtcgagtcaccatgtcaatagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtctattactgt gcgagagaggacgggtccggcagacgtcactttgactac IGA1 7 210 3 4 2 5 1 2 2 0 2 3 JY8QFUQ01CA2ZN 13228 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 967 94.44 204/216 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD6-19*01 F 1 8 8 13 8.8.13 [1.17.38.11] CAKGYSSGWYDFDCW in-frame - tctggattcacctttagcatctatgccatgagctgggtccgccaggctccagggaaggggctggaatgggtctcgactattagtggtagtggaaatagtacataccatgcagactccatgaagggccggttcaccctctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaaggggtatagcagtggctggtacgactttgactgctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [287] g107>t,S36>I(- - -)|g153>a|a162>g|g163>a,A55>T(- - -)|t186>a|g187>a,G63>N(- - -)|g188>a,G63>N(- - -)|c192>t|t199>c,Y67>H(+ - -)|c201>t,Y67>H(+ - -)|g211>a,V71>M(+ - -)|a232>c,I78>L(+ + +)| g107>t,S36>I(- - -)| g153>a|a162>g|g163>a,A55>T(- - -)| t186>a|g187>a,G63>N(- - -)|g188>a,G63>N(- - -)|c192>t| t199>c,Y67>H(+ - -)|c201>t,Y67>H(+ - -)|g211>a,V71>M(+ - -)|a232>c,I78>L(+ + +)| a318>g|a320>g| 317 (320) 221 (224) 209 (210) 12 (14) 4 (6) 8 1 (3) 5 2 1 1 0 0 1 0 0 1 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 51 51 48 3 2 1 1 2 0 0 0 0 0 0 0 0 0 0 30 24 20 4 2 2 0 2 1 0 0 0 0 1 0 0 0 0 117 114 110 4 0 4 0 1 1 1 1 0 0 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcacctttagcatctatgcc atgagctgggtccgccaggctccagggaaggggctggaatgggtctcgact attagtggtagtggaaatagtaca taccatgcagactccatgaagggccggttcaccctctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaggggtatagcagtggctggtacgactttgactgc AKGYSSGWYDFDC IGHV3-23 IGHD6-19 IGHJ4 ggattcacctttagcatctatgcc atgagctgggtccgccaggctccagggaaggggctggaatgggtctcgact attagtggtagtggaaatagtaca taccatgcagactccatgaagggccggttcaccctctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaggggtatagcagtggctggtacgactttgactgc IGG2 12 213 9 3 7 8 2 4 2 2 5 3 JY8QFUQ01CA830 19985 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 873 89.4 194/217 nt NA Homsap IGHJ5*02 F 237 96.08 49/51 nt Homsap IGHD6-19*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKSPLSVAGTRQNWFDPW in-frame + ctctgacttcacctttaatagctatgccatggcctgggtccgccaggctccagggaaggggctggagtgggtctcggctattggtgccagtggctacagcacatactacgcagactccgtcaagggccgcttcaccatctccagagaccattctaacagcacgctgcatctgcaaatgaacagcctgagagccgaagacacggccgtttattactgtgcgaaatcccctctatcagtggctggtacacgccaaaactggttcgacccctggggccagggaaccctggtcaccgtttcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [300] g80>a,G27>D(- - -)|a81>c,G27>D(- - -)|g104>a,S35>N(- - -)|c105>t,S35>N(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a162>g|a169>g,S57>G(+ + -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|t186>c|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|t189>c,G63>Y(+ - -)|g213>c|g225>c|a244>c,N82>H(- - -)|c249>t|g252>c,K84>N(+ - -)|a254>g,N85>S(- - -)|t262>c,Y88>H(+ - -)|g291>a|a303>t| g80>a,G27>D(- - -)|a81>c,G27>D(- - -)|g104>a,S35>N(- - -)|c105>t,S35>N(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a162>g| a169>g,S57>G(+ + -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|t186>c|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|t189>c,G63>Y(+ - -)| g213>c|g225>c|a244>c,N82>H(- - -)|c249>t|g252>c,K84>N(+ - -)|a254>g,N85>S(- - -)|t262>c,Y88>H(+ - -)|g291>a|a303>t| g319>t|a320>c| 318 (320) 223 (225) 200 23 (25) 7 (9) 16 4 4 2 4 2 (3) 0 1 0 5 0 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 2 1 0 1 0 0 0 0 0 0 0 51 51 48 3 1 2 2 0 0 0 0 0 0 0 1 0 0 0 30 24 17 7 1 6 1 1 0 3 0 0 0 0 1 0 1 0 117 114 105 9 5 4 1 1 1 1 1 0 1 0 3 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gacttcacctttaatagctatgcc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcggct attggtgccagtggctacagcaca tactacgcagactccgtcaagggccgcttcaccatctccagagaccattctaacagcacgctgcatctgcaaatgaacagcctgagagccgaagacacggccgtttattactgt gcgaaatcccctctatcagtggctggtacacgccaaaactggttcgacccc AKSPLSVAGTRQNWFDP IGHV3-23 IGHD6-19 IGHJ5 gacttcacctttaatagctatgcc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcggct attggtgccagtggctacagcaca tactacgcagactccgtcaagggccgcttcaccatctccagagaccattctaacagcacgctgcatctgcaaatgaacagcctgagagccgaagacacggccgtttattactgt gcgaaatcccctctatcagtggctggtacacgccaaaactggttcgacccc IGG1 23 213 14 9 6 12 8 11 6 1 6 10 JY8QFUQ01CABXM 16219 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 873 88.94 193/217 nt 88.48 192/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 15 8.8.15 [1.17.37.11] CAKDASRYYYNSYCDNW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 103 of V-REGION: 1 nucleotides (from position 214 in the user submitted sequence), (cause frameshift) cctctggattcacctttaccaactacgccatgagctgggttcgccaggttccagggaaggggctggagtgggtctcacttattagtgttcgtggcgatgacaccttctatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtatctgcaaatggacatcctgaaacccgaggacacggccgtttattttgcgcgaaagatgcgtctcgatattattataacagttattgtgacaattggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG2 93 100 [294] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|t111>c|c126>t|c134>t,A45>V(+ - +)|g163>c,A55>L(+ - +)|c164>t,A55>L(+ - +)|g173>t,G58>V(- - -)|a175>c,S59>R(- - -)|t186>c|g188>a,G63>D(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a195>c|a197>t,Y66>F(- + -)|c201>t|t246>c|a274>g,N92>D(+ + -)|g278>t,S93>I(- - -)|g284>a,R95>K(+ + +)|g286>c,A96>P(- - -)|a303>t|c309>t|t312>c| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|t111>c| c126>t|c134>t,A45>V(+ - +)|g163>c,A55>L(+ - +)|c164>t,A55>L(+ - +)| g173>t,G58>V(- - -)|a175>c,S59>R(- - -)|t186>c|g188>a,G63>D(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a195>c| a197>t,Y66>F(- + -)|c201>t|t246>c|a274>g,N92>D(+ + -)|g278>t,S93>I(- - -)|g284>a,R95>K(+ + +)|g286>c,A96>P(- - -)|a303>t|c309>t|t312>c| 320 225 201 24 9 15 2 4 5 4 2 0 2 0 3 0 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 0 1 0 0 0 0 1 0 0 0 51 51 47 4 1 3 0 0 3 0 0 0 0 0 1 0 0 0 30 24 17 7 2 5 1 2 0 1 2 0 0 0 0 0 1 0 117 113 103 10 5 5 1 1 2 2 0 0 2 0 1 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttaccaactacgcc atgagctgggttcgccaggttccagggaaggggctggagtgggtctcactt attagtgttcgtggcgatgacacc ttctatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtatctgcaaatggacatcctgaaacccgaggacacggccgtttattttgc gcgaaagatgcgtctcgatattattataacagttattgtgacaat AKDASRYYYNSYCDN IGHV3-23 IGHD3-22 IGHJ4 ggattcacctttaccaactacgcc atgagctgggttcgccaggttccagggaaggggctggagtgggtctcactt attagtgttcgtggcgatgacacc ttctatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtatctgcaaatggacatcctgaaacccgaggacacggccgtttattttgc gcgaaagatgcgtctcgatattattataacagttattgtgacaat IGG2 24 212 15 9 9 14 6 10 6 3 8 7 JY8QFUQ01CAI7Q 12956 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 889 88.74 197/222 nt 87.84 195/222 nt Homsap IGHJ3*01 F 205 90 45/50 nt Homsap IGHD6-19*01 F 2 10 7 16 10.7.16 [2.17.38.11] CTNDHSMAVSAKHAFDVW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'G' (1 nucleotides at position 16 to 16 in the user submitted sequence), (cause frameshift);in CDR1-IMGT at codon 34 of V-REGION: 'C' (1 nucleotides at position 24 to 24 in the user submitted sequence), (cause frameshift); gtctctggtgcctccGatgagcaCgtagtgcatactactggggctgggtccgccagacccccgggaaggggccggagtggattgggagtgtctactatggtgggagcacctactacaacccgtccctcaagagtcgggcctccatttctgtcgacacgtccaggaacgagttctccctgaggctgaactctgtgaccgccacagacacggctatgtattactgcacgaacgaccattcaatggcagtctctgctaagcatgcttttgatgtttggggtccagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [303] g83>c,G28>A(- + -)|c90>g,I30>M(+ + -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|t108>a,S36>A(- + -)|a124>g,I42>V(+ - +)|c133>a,P45>T(+ + -)|a138>c|t149>c,L50>P(- - -)|a166>g,I56>V(+ - +)|t171>c|a175>g,S59>G(+ + -)|a225>g|t227>c,V76>A(+ - +)|a229>t,T77>S(+ - +)|a234>t|c237>t|a240>c|a251>g,K84>R(+ + +)|c256>g,Q86>E(+ + -)|a269>g,K90>R(+ + +)|g275>a,S92>N(- - -)|g289>a,A97>T(- - -)|g301>a,V101>M(+ - -)|t312>c|g313>a,A105>T(- - -)| g83>c,G28>A(- + -)|c90>g,I30>M(+ + -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|t108>a,S36>A(- + -)| a124>g,I42>V(+ - +)|c133>a,P45>T(+ + -)|a138>c|t149>c,L50>P(- - -)| a166>g,I56>V(+ - +)|t171>c|a175>g,S59>G(+ + -)| a225>g|t227>c,V76>A(+ - +)|a229>t,T77>S(+ - +)|a234>t|c237>t|a240>c|a251>g,K84>R(+ + +)|c256>g,Q86>E(+ + -)|a269>g,K90>R(+ + +)|g275>a,S92>N(- - -)|g289>a,A97>T(- - -)|g301>a,V101>M(+ - -)|t312>c| g313>a,A105>T(- - -)|g317>a,R106>N(+ - -)|a318>c,R106>N(+ - -)|c319>g| 316 (320) 226 (230) 200 (201) 26 (29) 7 (8) 19 (21) 7 4 (5) 1 4 2 (3) 1 2 1 2 2 (3) 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 25 5 0 5 1 0 0 0 0 0 0 1 2 1 0 0 51 51 47 4 1 3 1 0 0 1 1 1 0 0 0 0 0 0 30 21 18 3 1 2 2 0 0 1 0 0 0 0 0 0 0 0 117 114 101 13 5 8 3 3 1 2 1 0 2 0 0 1 0 0 4 (8) 4 3 (4) 1 (4) 0 (1) 1 (3) 0 1 (2) 0 0 0 (1) 0 0 0 0 0 (1) 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct ggtgcctccatgagcagtagtgcatactac tggggctgggtccgccagacccccgggaaggggccggagtggattgggagt gtctactatggtgggagcacc tactacaacccgtccctcaagagtcgggcctccatttctgtcgacacgtccaggaacgagttctccctgaggctgaactctgtgaccgccacagacacggctatgtattactgc acgaacgaccattcaatggcagtctctgctaagcatgcttttgatgtt TNDHSMAVSAKHAFDV IGHV4-39 IGHD6-19 IGHJ3 ggtgcctccatgagcagtagtgcatactac tggggctgggtccgccagacccccgggaaggggccggagtggattgggagt gtctactatggtgggagcacc tactacaacccgtccctcaagagtcgggcctccatttctgtcgacacgtccaggaacgagttctccctgaggctgaactctgtgaccgccacagacacggctatgtattactgc acgaacgaccattcaatggcagtctctgctaagcatgcttttgatgtt IGA1 25 216 15 10 4 9 11 16 6 1 11 7 JY8QFUQ01CAN9N 3736 productive Homsap IGHV3-11*01 F 985 95.37 206/216 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD1-26*01 F 3 8 8 14 8.8.14 [1.17.38.11] CAKDRGKSGSYGLDHW in-frame + tctgggttcaccttcagtgactactacatgggctggatccgccaggctccagggaaggggctggagtggattgcgtacattagtggtagtggtgataccatatactacgcagactctgtgaagggccgattcaccatctccagggtcaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggccgtgtattactgtgcgaaagatcgggggaaaagtgggagctacggacttgaccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 89 [289, 288] a81>g|a118>g,S40>G(+ + -)|g157>a,V53>I(+ - +)|t160>g,S54>A(- + -)|a162>g,S54>A(- + -)|a172>g,S58>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a242>t,D81>V(- - -)|a263>c,Y88>S(+ - -)|g317>a,R106>K(+ + +)| a81>g| a118>g,S40>G(+ + -)|g157>a,V53>I(+ - +)|t160>g,S54>A(- + -)|a162>g,S54>A(- + -)| a172>g,S58>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)| a242>t,D81>V(- - -)|a263>c,Y88>S(+ - -)| g317>a,R106>K(+ + +)| 320 224 213 11 1 10 5 3 0 0 1 0 1 0 0 0 0 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 51 51 47 4 0 4 2 1 0 0 0 0 0 0 0 0 0 1 30 24 21 3 0 3 2 1 0 0 0 0 0 0 0 0 0 0 117 114 112 2 0 2 0 0 0 0 1 0 1 0 0 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct gggttcaccttcagtgactactac atgggctggatccgccaggctccagggaaggggctggagtggattgcgtac attagtggtagtggtgataccata tactacgcagactctgtgaagggccgattcaccatctccagggtcaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggccgtgtattactgt gcgaaagatcgggggaaaagtgggagctacggacttgaccac AKDRGKSGSYGLDH IGHV3-11 IGHD1-26 IGHJ4 gggttcaccttcagtgactactac atgggctggatccgccaggctccagggaaggggctggagtggattgcgtac attagtggtagtggtgataccata tactacgcagactctgtgaagggccgattcaccatctccagggtcaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggccgtgtattactgt gcgaaagatcgggggaaaagtgggagctacggacttgaccac IGA2 10 213 7 3 2 2 5 8 0 1 6 3 JY8QFUQ01CAR29 3371 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 993 96.26 206/214 nt 95.33 204/214 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 7 8 8.7.8 [1.16.38.11] CARDNYGDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 49 of V-REGION: 1 nucleotides (from position 59 in the user submitted sequence), (cause frameshift)in CDR2-IMGT, from codon 63 of V-REGION: 1 nucleotides (from position 93 in the user submitted sequence), (cause frameshift) tctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaagggctggtgtgggtctcacgtgttaatggtgatggggtagcacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccag NA IGA2 93 100 [269, 270] a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| 320 222 214 8 4 4 3 1 0 0 0 0 0 0 3 0 1 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 50 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 23 21 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 4 2 1 1 0 0 0 0 0 0 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaagggctggtgtgggtctcacgt gttaatggtgatggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaagggctggtgtgggtctcacgt gttaatggtgatggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac IGA2 8 211 4 4 1 5 3 3 4 0 2 2 JY8QFUQ01CB275 5924 productive (see comment) Homsap IGHV2-5*02 F 1165 97.94 238/243 nt 97.53 237/243 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD5-12*01 F 2 10 7 11 10.7.11 [9.16.38.11] CAHRRIRFGFDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 45 of V-REGION: 1 nucleotides (from position 79 in the user submitted sequence), (cause frameshift) gaccctcacgctgacctgcaccttctctgggttctcactcagcactggtggagtgggtgtgggctggatccgtcagccccaggaaaggccctggagtggcttgcactcatttattgggatgatgataagcgctacagcccatctctgaagagcagactcaccatcaccaaggacacgtccaagaaccaggtggtcctcacaatgaccaacatggaccctgtggacacagccacatattactgtgcacacaggaggatacgattcggctttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaagggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 94 [307, 308] a103>g,S35>G(+ + -)|g225>a|c246>g|a252>g|t267>c| a103>g,S35>G(+ + -)| g225>a|c246>g|a252>g|t267>c| a321>g|c322>a| 320 (322) 251 (253) 246 5 (7) 4 (6) 1 2 (3) 1 0 1 0 0 (1) 0 0 0 1 0 0 78 28 28 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 29 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 50 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 110 4 4 0 1 1 0 1 0 0 0 0 0 1 0 0 8 (10) 8 8 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 0 aa,13-14(FR1)|aa,40-41(FR1)|ta,102-103(CDR1)|aa,141-142(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,238-239(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,267-268(FR3)|aa,270-271(FR3)|aa,277-278(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,10-11(FR1)|tt,73-74(FR1)|tt,82-83(CDR1)|ta,102-103(CDR1)|tt,158-159(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,80-83(CDR1)|ggct,118-121(FR2)|ggct,155-158(FR2)|agca,220-223(FR3)|ggct,224-227(FR3)| aacc,41-44(FR1)|agcc,131-134(FR2)|agcc,202-205(FR3)|aacc,253-256(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)| gaccctcacgctgacctgcaccttctct gggttctcactcagcactggtggagtgggt gtgggctggatccgtcagccccaggaaaggccctggagtggcttgcactc atttattgggatgatgataag cgctacagcccatctctgaagagcagactcaccatcaccaaggacacgtccaagaaccaggtggtcctcacaatgaccaacatggaccctgtggacacagccacatattactgt gcacacaggaggatacgattcggctttgactac AHRRIRFGFDY IGHV2-5 IGHD5-12 IGHJ4 gggttctcactcagcactggtggagtgggt gtgggctggatccgtcagccccaggaaaggccctggagtggcttgcactc atttattgggatgatgataag cgctacagcccatctctgaagagcagactcaccatcaccaaggacacgtccaagaaccaggtggtcctcacaatgaccaacatggaccctgtggacacagccacatattactgt gcacacaggaggatacgattcggctttgactac IGG1 5 215 4 1 1 2 3 3 4 0 0 1 JY8QFUQ01CB2DX 12709 productive Homsap IGHV3-73*01 F, or Homsap IGHV3-73*02 F 1105 100 222/222 nt NA Homsap IGHJ6*02 F 283 95.16 59/62 nt Homsap IGHD6-6*01 F 2 8 10 16 8.10.16 [1.17.38.11] CTRRSIADNFYYYGMDVW in-frame - tctgggttcaccttcagtggctctgctatgcactgggtccgccaggcttccgggaaagggctggagtgggttggccgtattagaagcaaagctaacagttacgcgacagcatatgctgcgtcggtgaaaggcaggttcaccatctccagagatgattcaaagaacacggcgtatctgcaaatgaacagcctgaaaaccgaggacacggccgtgtattactgtactagacggagtatagcagataacttctactactacggtatggacgtctggggccaagggaccacagtcaccgtctcctcagcttccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 93 100 [302] a320>g| 319 (320) 229 (230) 229 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,58-59(FR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,114-115(CDR1)|tt,135-136(FR2)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|ggct,106-109(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|agca,195-198(CDR2)|ggca,220-223(FR3)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,59-62(FR1)|agcc,72-75(FR1)|tgct,111-114(CDR1)|agct,177-180(CDR2)|tgct,201-204(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| tct gggttcaccttcagtggctctgct atgcactgggtccgccaggcttccgggaaagggctggagtgggttggccgt attagaagcaaagctaacagttacgcgaca gcatatgctgcgtcggtgaaaggcaggttcaccatctccagagatgattcaaagaacacggcgtatctgcaaatgaacagcctgaaaaccgaggacacggccgtgtattactgt actagacggagtatagcagataacttctactactacggtatggacgtc TRRSIADNFYYYGMDV IGHV3-73 IGHD6-6 IGHJ6 gggttcaccttcagtggctctgct atgcactgggtccgccaggcttccgggaaagggctggagtgggttggccgt attagaagcaaagctaacagttacgcgaca gcatatgctgcgtcggtgaaaggcaggttcaccatctccagagatgattcaaagaacacggcgtatctgcaaatgaacagcctgaaaaccgaggacacggccgtgtattactgt actagacggagtatagcagataacttctactactacggtatggacgtc IGG2 0 219 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01CBC07 14783 productive (see comment) Homsap IGHV4-4*02 F, or Homsap IGHV4-4*03 F or Homsap IGHV4-4*04 F or Homsap IGHV4-4*05 F (see comment) 662 77.98 170/218 nt 77.06 168/218 nt Homsap IGHJ4*02 F (see comment) 168 83.33 40/48 nt Homsap IGHD3-3*02 F 3 9 7 12 9.7.12 [1.17.38.11] CAREDIIREGLDRW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (77,98% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ1*01 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 39 of V-REGION: 'C' (1 nucleotides at position 34 to 34 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 45 of V-REGION: 'A' (1 nucleotides at position 52 to 52 in the user submitted sequence), (cause frameshift); tgtctggtggttctactcgcactaaccattggtCggaattgggtccgccagAccccccgacaagggactggagtggattggagaagtctataagagtggacagaccaactacaacccgtcactccagagtcgcgttgacctcttcattgacaactccaggaatcagatctccctaaatatgagagatgtgaccgccgcggacacggccgtctattactgcgcgagagaagacattattcgggagggccttgaccgctggggccagggcaccctggtcaccgtgtcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] c75>g|c84>t|c87>t|t89>c,I30>T(- - -)|c90>t,I30>T(- - -)|a91>c,S31>R(- - -)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|t108>c,S36>N(- - -)|a109>c,N37>H(- - -)|c111>t,N37>H(- - -)|g119>a,S40>N(- - -)|a138>c|g140>a,G47>D(- - -)|g141>c,G47>D(- - -)|g147>a|g162>a|a166>g,I56>V(+ - +)|c172>a,H58>K(- - +)|t174>g,H58>K(- - +)|g189>a|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>g,S64>Q(- - -)|c210>a|a214>c,K72>Q(+ - -)|a225>c|c228>t|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|a232>c,I78>L(+ + +)|a234>c,I78>L(+ + +)|c236>t,S79>F(- - -)|a237>c,S79>F(- - -)|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|g246>c,K82>N(+ - -)|a251>g,K84>R(+ + +)|c255>t|t259>a,F87>I(+ - -)|g267>a|g270>t,K90>N(+ - -)|c271>a,L91>M(+ + -)|c276>a,S92>R(- - -)|t277>g,S93>D(- - -)|c278>a,S93>D(- - -)|g303>c|t312>c| c75>g| c84>t|c87>t|t89>c,I30>T(- - -)|c90>t,I30>T(- - -)|a91>c,S31>R(- - -)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|t108>c,S36>N(- - -)|a109>c,N37>H(- - -)|c111>t,N37>H(- - -)| g119>a,S40>N(- - -)|a138>c|g140>a,G47>D(- - -)|g141>c,G47>D(- - -)|g147>a|g162>a| a166>g,I56>V(+ - +)|c172>a,H58>K(- - +)|t174>g,H58>K(- - +)|g189>a|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>g,S64>Q(- - -)| c210>a|a214>c,K72>Q(+ - -)|a225>c|c228>t|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|a232>c,I78>L(+ + +)|a234>c,I78>L(+ + +)|c236>t,S79>F(- - -)|a237>c,S79>F(- - -)|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|g246>c,K82>N(+ - -)|a251>g,K84>R(+ + +)|c255>t|t259>a,F87>I(+ - -)|g267>a|g270>t,K90>N(+ - -)|c271>a,L91>M(+ + -)|c276>a,S92>R(- - -)|t277>g,S93>D(- - -)|c278>a,S93>D(- - -)|g303>c|t312>c| 320 226 178 48 14 34 3 9 7 3 9 6 1 1 4 2 1 2 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 27 17 10 2 8 0 1 4 2 2 0 0 0 1 0 0 0 51 51 45 6 3 3 0 4 0 0 1 0 0 0 1 0 0 0 30 21 14 7 1 6 1 2 0 0 1 1 0 0 0 1 0 1 117 114 90 24 7 17 2 2 3 1 5 5 1 1 2 0 1 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tgtct ggtggttctactcgcactaaccattgg tggaattgggtccgccagccccccgacaagggactggagtggattggagaa gtctataagagtggacagacc aactacaacccgtcactccagagtcgcgttgacctcttcattgacaactccaggaatcagatctccctaaatatgagagatgtgaccgccgcggacacggccgtctattactgc gcgagagaagacattattcgggagggccttgaccgc AREDIIREGLDR IGHV4-4 IGHD3-3 IGHJ4 ggtggttctactcgcactaaccattgg tggaattgggtccgccagccccccgacaagggactggagtggattggagaa gtctataagagtggacagacc aactacaacccgtcactccagagtcgcgttgacctcttcattgacaactccaggaatcagatctccctaaatatgagagatgtgaccgccgcggacacggccgtctattactgc gcgagagaagacattattcgggagggccttgaccgc IGA1 47 213 22 25 16 28 6 19 10 3 20 14 JY8QFUQ01CBLRN 22154 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 905 90.83 198/218 nt 90.37 197/218 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD5-24*01 ORF 1 8 8 12 8.8.12 [1.17.38.11] CARDRLTISALDYW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'T' (1 nucleotides at position 16 to 16 in the user submitted sequence), (cause frameshift); cctctggattcacctTttaacaactacgccatgtcctgggtccgccaggctccagggaaggggcttgagtgggtctcagctataactgatagcggtctttacacatactacgcagactccgtgaggggccggttcaccgtctccagagacacttccaagaacacgctgtttctgcaaatggacagcctgagagccgaggacacggccgtatatttctgtgcgagggatagactcacgatttcagcccttgattactggggccagggagccctggtcaccgtctcctcagcttccaccaagggcccatcggtcttcccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactc NA IGG3 100 100 [288, 287] g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|t111>c|a118>t|g119>c|g150>t|t168>a|g170>c,S57>T(+ - +)|g173>a,G58>D(- - -)|t177>c|g187>c,G63>L(- - -)|g188>t,G63>L(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|a245>c,N82>T(- + -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|a308>t,Y103>F(- + -)| g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|t111>c| a118>t|g119>c|g150>t| t168>a|g170>c,S57>T(+ - +)|g173>a,G58>D(- - -)|t177>c|g187>c,G63>L(- - -)|g188>t,G63>L(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)| a215>g,K72>R(+ + +)|a232>g,I78>V(+ - +)|a245>c,N82>T(- + -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|a308>t,Y103>F(- + -)| a317>g,K106>R(+ + +)|a318>g,K106>R(+ + +)| 316 (320) 222 (226) 202 (204) 20 (22) 6 14 (16) 3 (5) 4 0 2 1 0 4 1 3 0 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 2 0 1 0 0 0 0 0 0 0 0 51 51 48 3 3 0 0 0 0 0 0 0 1 0 1 0 1 0 30 24 16 8 2 6 0 2 0 1 0 0 1 1 2 0 1 0 117 114 108 6 0 6 3 0 0 0 1 0 2 0 0 0 0 0 4 (8) 4 4 (6) 0 (2) 0 0 (2) 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttaacaactacgcc atgtcctgggtccgccaggctccagggaaggggcttgagtgggtctcagct ataactgatagcggtctttacaca tactacgcagactccgtgaggggccggttcaccgtctccagagacacttccaagaacacgctgtttctgcaaatggacagcctgagagccgaggacacggccgtatatttctgt gcgagggatagactcacgatttcagcccttgattac ARDRLTISALDY IGHV3-23 IGHD5-24 IGHJ4 ggattcacctttaacaactacgcc atgtcctgggtccgccaggctccagggaaggggcttgagtgggtctcagct ataactgatagcggtctttacaca tactacgcagactccgtgaggggccggttcaccgtctccagagacacttccaagaacacgctgtttctgcaaatggacagcctgagagccgaggacacggccgtatatttctgt gcgagggatagactcacgatttcagcccttgattac IGG3 20 213 9 11 4 9 5 11 3 3 6 8 JY8QFUQ01CBORW 10923 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 860 88.53 193/218 nt 88.07 192/218 nt Homsap IGHJ6*02 F 193 79.03 49/62 nt Homsap IGHD6-6*01 F 1 8 8 17 8.8.17 [1.17.38.11] CARDSGSSDYTRYDAQDVW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 72 of V-REGION: 'G' (1 nucleotides at position 124 to 124 in the user submitted sequence), (cause frameshift); cctctggattcagttttagttcttatggcatgaactgggtccgccaggctccatggggggggctggagtgggtctcattcattaacagtgttagtagttacaaatactatgtggacccagtgaGggggccgattcaccatctccagagacaacgccaagaacgcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtttactactgtgcgagagatagcggctcgtccgactacactaggtacgacgcccaggacgtctggggccaagggaccgcggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [302] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>t|a106>t|g107>c|c108>t|a112>g,S38>G(+ + -)|g139>t,G47>W(- - -)|a142>g,K48>G(- - -)|a143>g,K48>G(- - -)|c164>t,S55>F(- - -)|g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|t194>a,I65>K(- + -)|c201>t|c203>t,A68>V(+ - +)|a204>g,A68>V(+ - +)|t208>c,S70>P(+ - -)|a215>g,K72>R(+ + +)|t256>g,S86>A(- + -)|c265>t|g303>t|t306>c| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>t|a106>t|g107>c|c108>t|a112>g,S38>G(+ + -)| g139>t,G47>W(- - -)|a142>g,K48>G(- - -)|a143>g,K48>G(- - -)|c164>t,S55>F(- - -)| g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|t194>a,I65>K(- + -)| c201>t|c203>t,A68>V(+ - +)|a204>g,A68>V(+ - +)|t208>c,S70>P(+ - -)|a215>g,K72>R(+ + +)|t256>g,S86>A(- + -)|c265>t|g303>t|t306>c| 320 226 201 25 8 17 6 1 7 3 0 0 1 1 1 1 3 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 4 3 1 0 3 0 0 0 1 0 1 1 0 0 51 51 47 4 0 4 2 0 1 0 0 0 0 0 0 0 1 0 30 24 19 5 0 5 1 1 0 1 0 0 0 1 0 0 1 0 117 114 105 9 4 5 2 0 3 2 0 0 0 0 0 0 1 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcagttttagttcttatggc atgaactgggtccgccaggctccatggggggggctggagtgggtctcattc attaacagtgttagtagttacaaa tactatgtggacccagtgaggggccgattcaccatctccagagacaacgccaagaacgcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtttactactgt gcgagagatagcggctcgtccgactacactaggtacgacgcccaggacgtc ARDSGSSDYTRYDAQDV IGHV3-21 IGHD6-6 IGHJ6 ggattcagttttagttcttatggc atgaactgggtccgccaggctccatggggggggctggagtgggtctcattc attaacagtgttagtagttacaaa tactatgtggacccagtgaggggccgattcaccatctccagagacaacgccaagaacgcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtttactactgt gcgagagatagcggctcgtccgactacactaggtacgacgcccaggacgtc IGG2 25 213 17 8 8 13 9 12 4 4 9 8 JY8QFUQ01CBST2 6180 productive Homsap IGHV3-11*01 F 929 91.4 202/221 nt NA Homsap IGHJ4*02 F (see comment) 186 87.5 42/48 nt Homsap IGHD6-13*01 F 1 8 8 15 8.8.15 [2.17.38.11] CARVKYSSSWSSILDSW in-frame - Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) cagcctctggattcaacttcggtgactactacatgaactggatccgccaggctccagggaaggggctggagtgggtttcattcattagtagtcctggtatcaggagtttctacgcagactctgtgaagggccgattcactatttccagggacaacgccaagaactcattgtatctgcaaatgaacagcctgagagtcgaggacacggccatatattactgtgcgagagtgaaatatagcagcagctggtcctcaatccttgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] c86>a,T29>N(- + -)|a103>g,S35>G(+ + -)|g119>a,S40>N(- - -)|a164>t,Y55>F(- + -)|a175>c,S59>P(+ - -)|g176>c,S59>P(+ - -)|g188>t,S63>I(- - -)|t189>c,S63>I(- - -)|c191>g,T64>R(- - -)|c192>g,T64>R(- - -)|t194>g,I65>S(- - -)|a195>t,I65>S(- - -)|a197>t,Y66>F(- + -)|c231>t|c234>t|c259>t|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| c86>a,T29>N(- + -)|a103>g,S35>G(+ + -)| g119>a,S40>N(- - -)|a164>t,Y55>F(- + -)| a175>c,S59>P(+ - -)|g176>c,S59>P(+ - -)|g188>t,S63>I(- - -)|t189>c,S63>I(- - -)|c191>g,T64>R(- - -)|c192>g,T64>R(- - -)|t194>g,I65>S(- - -)|a195>t,I65>S(- - -)| a197>t,Y66>F(- + -)|c231>t|c234>t|c259>t|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| a320>t| 319 (320) 228 (229) 209 19 (20) 3 (4) 16 1 3 4 1 1 1 3 (4) 0 1 2 1 1 78 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 1 0 0 0 0 0 0 51 51 49 2 0 2 0 1 0 0 0 0 1 0 0 0 0 0 30 24 16 8 0 8 0 0 0 1 1 0 1 0 1 2 1 1 117 114 107 7 3 4 0 2 4 0 0 0 1 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cagcctct ggattcaacttcggtgactactac atgaactggatccgccaggctccagggaaggggctggagtgggtttcattc attagtagtcctggtatcaggagt ttctacgcagactctgtgaagggccgattcactatttccagggacaacgccaagaactcattgtatctgcaaatgaacagcctgagagtcgaggacacggccatatattactgt gcgagagtgaaatatagcagcagctggtcctcaatccttgactcc ARVKYSSSWSSILDS IGHV3-11 IGHD6-13 IGHJ4 ggattcaacttcggtgactactac atgaactggatccgccaggctccagggaaggggctggagtgggtttcattc attagtagtcctggtatcaggagt ttctacgcagactctgtgaagggccgattcactatttccagggacaacgccaagaactcattgtatctgcaaatgaacagcctgagagtcgaggacacggccatatattactgt gcgagagtgaaatatagcagcagctggtcctcaatccttgactcc IGA1 19 213 9 10 7 12 2 7 3 0 6 10 JY8QFUQ01CBUHR 20878 productive Homsap IGHV4-61*02 F (see comment) 650 77.38 171/221 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-3*01 F 3 10 7 16 10.7.16 [1.17.38.11] CARERDRSIGGAAELDSW in-frame + Low V-REGION identity (77,38% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tctccaatgtacccatcagtcgtggaggttactactggaattggatccggcaggccgccgggaaggggctggagtggcttggacgtgttgacagtaatggattcgtcaggtacaattcttccctcaaaagtcgcctttctatgtcactagacacgtccaagaatcagatctccctgatattgaggtctgtgatcgccgcagacacggccgtatatttctgtgcgagggagcgtgataggtcgatcggtggagcggcagaacttgactcctggggccagggatccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [300] t78>c|g79>a,G27>N(- - -)|g80>a,G27>N(- - -)|g83>t,G28>V(- - -)|c84>a,G28>V(- - -)|t85>c,S29>P(+ - -)|c93>t|a100>c,S34>R(- - -)|t105>a|a106>g,S36>G(+ + -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|c133>g,P45>A(- - -)|a147>g|a157>c,I53>L(+ + +)|g162>a|a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|t169>g,Y57>D(- - -)|t171>c,Y57>D(- - -)|c173>g,T58>S(+ - +)|c174>t,T58>S(+ - +)|g176>a,S59>N(- - -)|g189>a|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|a197>g,N66>R(+ - -)|c198>g,N66>R(+ - -)|c204>t|c205>t,P69>S(+ - -)|c207>t,P69>S(+ - -)|g216>a|a225>c|g226>c,V76>L(+ - +)|c228>t,V76>L(+ - +)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|g238>c,V80>L(+ - +)|c255>t|t259>a,F87>I(+ - -)|a269>t,K90>I(- + -)|g270>a,K90>I(- + -)|c271>t|c276>g,S92>R(- - -)|c284>t,T95>I(- - -)|g303>a|a308>t,Y103>F(- + -)|a318>g| t78>c| g79>a,G27>N(- - -)|g80>a,G27>N(- - -)|g83>t,G28>V(- - -)|c84>a,G28>V(- - -)|t85>c,S29>P(+ - -)|c93>t|a100>c,S34>R(- - -)|t105>a|a106>g,S36>G(+ + -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|c133>g,P45>A(- - -)|a147>g|a157>c,I53>L(+ + +)|g162>a| a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|t169>g,Y57>D(- - -)|t171>c,Y57>D(- - -)|c173>g,T58>S(+ - +)|c174>t,T58>S(+ - +)|g176>a,S59>N(- - -)|g189>a|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)| a197>g,N66>R(+ - -)|c198>g,N66>R(+ - -)|c204>t|c205>t,P69>S(+ - -)|c207>t,P69>S(+ - -)|g216>a|a225>c|g226>c,V76>L(+ - +)|c228>t,V76>L(+ - +)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|g238>c,V80>L(+ - +)|c255>t|t259>a,F87>I(+ - -)|a269>t,K90>I(- + -)|g270>a,K90>I(- + -)|c271>t|c276>g,S92>R(- - -)|c284>t,T95>I(- - -)|g303>a|a308>t,Y103>F(- + -)| a318>g| 320 229 178 51 13 38 7 9 13 3 3 1 4 2 2 4 2 1 78 5 4 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 30 21 9 2 7 1 2 1 1 1 1 0 1 0 0 1 0 51 51 45 6 2 4 1 2 1 0 1 0 0 0 0 1 0 0 30 21 9 12 1 11 2 2 3 1 0 0 1 0 0 1 1 1 117 114 92 22 6 16 2 3 8 0 1 0 3 1 2 2 0 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctcc aatgtacccatcagtcgtggaggttactac tggaattggatccggcaggccgccgggaaggggctggagtggcttggacgt gttgacagtaatggattcgtc aggtacaattcttccctcaaaagtcgcctttctatgtcactagacacgtccaagaatcagatctccctgatattgaggtctgtgatcgccgcagacacggccgtatatttctgt gcgagggagcgtgataggtcgatcggtggagcggcagaacttgactcc ARERDRSIGGAAELDS IGHV4-61 IGHD3-3 IGHJ4 aatgtacccatcagtcgtggaggttactac tggaattggatccggcaggccgccgggaaggggctggagtggcttggacgt gttgacagtaatggattcgtc aggtacaattcttccctcaaaagtcgcctttctatgtcactagacacgtccaagaatcagatctccctgatattgaggtctgtgatcgccgcagacacggccgtatatttctgt gcgagggagcgtgataggtcgatcggtggagcggcagaacttgactcc IGA1 49 216 30 19 22 31 8 18 8 3 20 18 JY8QFUQ01CC9VV 8386 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [1.17.38.11] CTRDIGGRYSYW in-frame + ctccggattcacctttagtaattactggatgcactgggtccgccaagctccaggggagggactggtgtgggtctcacggactaatgaagatgggagtatcacaaactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtttctgcaaatgaacagtctcagagtcgaggacacggctgtttattactgtacaagagacatcggtgggcgctactcctactggggccagggagtcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [278] t78>c|c90>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a142>g,K48>E(+ - -)|g147>a|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|a263>t,Y88>F(- + -)|g282>c|c287>t,A96>V(+ - +)|g303>t|g313>a,A105>T(- - -)| t78>c| c90>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a142>g,K48>E(+ - -)|g147>a|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|a263>t,Y88>F(- + -)|g282>c|c287>t,A96>V(+ - +)|g303>t| g313>a,A105>T(- - -)| 320 225 204 21 6 15 3 6 3 2 0 0 1 1 1 1 2 1 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 2 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 1 1 0 0 0 0 0 0 0 0 0 1 30 24 19 5 0 5 1 1 0 1 0 0 0 1 0 0 1 0 117 114 106 8 2 6 1 2 1 0 0 0 1 0 1 1 1 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcacctttagtaattactgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtttctgcaaatgaacagtctcagagtcgaggacacggctgtttattactgt acaagagacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttagtaattactgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtttctgcaaatgaacagtctcagagtcgaggacacggctgtttattactgt acaagagacatcggtgggcgctactcctac IGA2 19 213 12 7 8 12 4 7 4 1 7 7 JY8QFUQ01CCN00 18156 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 955 93.15 204/219 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD4-23*01 ORF 2 8 8 13 8.8.13 [2.17.38.11] CARGGGGKSIPLDNW in-frame - gcgtctggcttcacgttcagtagttatggcatgcaatgggtccgccaggctccaggcaaggggctggagtgggtggcagttatttggtatgatggaagtaataaatattatgcagactccgtgaagggccgattcgacatctccagagacaattccaagaacacactatatctgcaaatgaacaacctgagagccgaggacacggctatgtaccattgtgcgagaggcggcggtggtaagtccattccacttgacaactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [289] a81>c|c87>g|c108>t|c120>a,H40>Q(- + -)|a168>t|c198>t|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|g258>a|g261>a|g278>a,S93>N(- - -)|g301>a,V101>M(+ - -)|t306>c|t307>c,Y103>H(+ - -)|c309>t,Y103>H(+ - -)| a81>c|c87>g|c108>t| c120>a,H40>Q(- + -)| a168>t| c198>t|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|g258>a|g261>a|g278>a,S93>N(- - -)|g301>a,V101>M(+ - -)|t306>c|t307>c,Y103>H(+ - -)|c309>t,Y103>H(+ - -)| a320>g| 319 (320) 226 (227) 211 15 (16) 8 (9) 7 1 (2) 4 3 2 1 2 1 0 0 1 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 3 0 0 0 1 0 1 0 0 0 0 1 0 0 51 51 50 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 30 24 23 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 117 114 104 10 4 6 1 4 2 2 0 1 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcgtct ggcttcacgttcagtagttatggc atgcaatgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atttggtatgatggaagtaataaa tattatgcagactccgtgaagggccgattcgacatctccagagacaattccaagaacacactatatctgcaaatgaacaacctgagagccgaggacacggctatgtaccattgt gcgagaggcggcggtggtaagtccattccacttgacaac ARGGGGKSIPLDN IGHV3-33 IGHD4-23 IGHJ4 ggcttcacgttcagtagttatggc atgcaatgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atttggtatgatggaagtaataaa tattatgcagactccgtgaagggccgattcgacatctccagagacaattccaagaacacactatatctgcaaatgaacaacctgagagccgaggacacggctatgtaccattgt gcgagaggcggcggtggtaagtccattccacttgacaac IGA1 15 213 10 5 7 10 3 5 4 4 7 0 JY8QFUQ01CCO0W 20127 productive Homsap IGHV3-30*01 F 891 90.32 196/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-16*01 F 1 8 8 9 8.8.9 [1.17.38.11] CAREGGGFDYW in-frame - ctctggattcagtttcagtagctatgcaatgcattgggtccgccaggctccaggcaaggggctagagtgggtgacagttatatcagctgatgcaactaccagtcactacgcagactccgtgaagggccgattcacccactccagagacaattccaagaacacgctgtctctgcaaatggacagcctgagacctgaagacacggctgtatattactgtgcgagagaggggggcggatttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggccccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 94 [277] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|t114>a|c120>t|g160>a,A54>T(- - -)|t172>g,Y58>A(- - -)|a173>c,Y58>A(- - -)|g185>c,G62>A(- + -)|g188>c,S63>T(+ - +)|a191>c,N64>T(- + -)|t192>c,N64>T(- + -)|a194>g,K65>S(- - -)|a195>t,K65>S(- - -)|t196>c,Y66>H(+ - -)|a232>c,I78>H(- - -)|t233>a,I78>H(- - -)|a263>c,Y88>S(+ - -)|a274>g,N92>D(+ + -)|g286>c,A96>P(- - -)|g291>a|g303>a| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|t114>a| c120>t|g160>a,A54>T(- - -)| t172>g,Y58>A(- - -)|a173>c,Y58>A(- - -)|g185>c,G62>A(- + -)|g188>c,S63>T(+ - +)|a191>c,N64>T(- + -)|t192>c,N64>T(- + -)|a194>g,K65>S(- - -)|a195>t,K65>S(- - -)| t196>c,Y66>H(+ - -)|a232>c,I78>H(- - -)|t233>a,I78>H(- - -)|a263>c,Y88>S(+ - -)|a274>g,N92>D(+ + -)|g286>c,A96>P(- - -)|g291>a|g303>a| 320 225 204 21 4 17 2 3 2 2 4 0 1 2 3 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 1 0 0 0 0 1 0 1 0 0 51 51 49 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 30 24 16 8 0 8 1 0 0 1 2 0 1 0 2 0 0 1 117 114 106 8 2 6 1 2 0 1 2 0 0 1 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagtttcagtagctatgca atgcattgggtccgccaggctccaggcaaggggctagagtgggtgacagtt atatcagctgatgcaactaccagt cactacgcagactccgtgaagggccgattcacccactccagagacaattccaagaacacgctgtctctgcaaatggacagcctgagacctgaagacacggctgtatattactgt gcgagagaggggggcggatttgactac AREGGGFDY IGHV3-30 IGHD3-16 IGHJ4 ggattcagtttcagtagctatgca atgcattgggtccgccaggctccaggcaaggggctagagtgggtgacagtt atatcagctgatgcaactaccagt cactacgcagactccgtgaagggccgattcacccactccagagacaattccaagaacacgctgtctctgcaaatggacagcctgagacctgaagacacggctgtatattactgt gcgagagaggggggcggatttgactac IGG1 21 213 9 12 5 9 4 12 3 1 7 10 JY8QFUQ01CCV7N 19783 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 775 84.02 184/219 nt 83.56 183/219 nt Homsap IGHJ3*02 F (see comment) 142 76 38/50 nt Homsap IGHD1-1*01 F 3 8 8 17 8.8.17 [2.16.38.11] CAKDIWAREWNDMAAFQMW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (84,02% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ1*01 and Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) in FR2-IMGT, from codon 45 of V-REGION: 1 nucleotides (from position 52 in the user submitted sequence), (cause frameshift) cgcccccggaatcacttttcccagctatgtcatgagttgggtccgccaggcccagggaaggggctggagtgggtcgcaagcattagtgccaggggcgacagttcatactacgcagactccgtgaagggccgattcaccatctccagggacaatcccaggaccacattgtatctggaaatgaacagtctgagagtcgaagacacggccacatattattgtgcgaaagatatctgggcgcgagagtggaacgacatggcggcttttcagatgtggggccaggggacacttgtcattgtctcgtcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [302] a72>c|t76>c,S26>P(+ - -)|t78>c,S26>P(+ - -)|t82>a,F28>I(+ - -)|c87>t|a103>c,S35>P(+ - -)|g104>c,S35>P(+ - -)|c113>t,A38>V(+ - +)|c120>t|t160>g,S54>A(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t165>c,A55>S(- + -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|t177>g,S59>R(- - -)|t186>c|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|c192>t|a193>t,T65>S(+ - +)|g225>a|a240>g|t247>c,S83>P(+ - -)|a251>g,K84>R(+ + +)|a254>c,N85>T(- + -)|g258>a|c259>t|c268>g,Q90>E(+ + -)|c279>t|c287>t,A96>V(+ - +)|g291>a|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|c309>t| a72>c|t76>c,S26>P(+ - -)|t78>c,S26>P(+ - -)| t82>a,F28>I(+ - -)|c87>t|a103>c,S35>P(+ - -)|g104>c,S35>P(+ - -)|c113>t,A38>V(+ - +)| c120>t|t160>g,S54>A(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t165>c,A55>S(- + -)| g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|t177>g,S59>R(- - -)|t186>c|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|c192>t|a193>t,T65>S(+ - +)| g225>a|a240>g|t247>c,S83>P(+ - -)|a251>g,K84>R(+ + +)|a254>c,N85>T(- + -)|g258>a|c259>t|c268>g,Q90>E(+ + -)|c279>t|c287>t,A96>V(+ - +)|g291>a|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|c309>t| 320 227 192 35 12 23 2 6 8 8 3 0 1 1 2 2 0 2 78 7 4 3 1 2 0 0 0 2 1 0 0 0 0 0 0 0 36 24 19 5 1 4 0 0 2 0 1 0 0 1 1 0 0 0 51 50 45 5 1 4 0 1 1 1 0 0 0 0 0 1 0 1 30 24 16 8 2 6 0 1 1 3 0 0 1 0 1 0 0 1 117 114 100 14 7 7 2 4 4 2 1 0 0 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cgccccc ggaatcacttttcccagctatgtc atgagttgggtccgccaggcccagggaaggggctggagtgggtcgcaagc attagtgccaggggcgacagttca tactacgcagactccgtgaagggccgattcaccatctccagggacaatcccaggaccacattgtatctggaaatgaacagtctgagagtcgaagacacggccacatattattgt gcgaaagatatctgggcgcgagagtggaacgacatggcggcttttcagatg AKDIWAREWNDMAAFQM IGHV3-23 IGHD1-1 IGHJ3 ggaatcacttttcccagctatgtc atgagttgggtccgccaggcccagggaaggggctggagtgggtcgcaagc attagtgccaggggcgacagttca tactacgcagactccgtgaagggccgattcaccatctccagggacaatcccaggaccacattgtatctggaaatgaacagtctgagagtcgaagacacggccacatattattgt gcgaaagatatctgggcgcgagagtggaacgacatggcggcttttcagatg IGG1 32 212 22 10 14 18 8 14 8 3 11 10 JY8QFUQ01CCYR6 3713 productive Homsap IGHV1-3*01 F 849 88.79 190/214 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 174 82.35 42/51 nt Homsap IGHD2-8*02 F 2 8 8 13 8.8.13 [0.17.38.11] CARDGYLPGNGADFW in-frame + Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) tggatacagcttcactaatcacattatccattgggtgcgccaggcccccggacaagggcttgagtgggtggggtcgatcaacgctggcaatggcaatacaagatattcacagaagttgcagggcagagtcaccatttccagggacacatccgcgagcatcgccaacatggagttgagcagcctgagatatgaagacacggctgtatattattgtgcgagagacggctatttgcctggcaacggggctgacttttggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)|g112>a,A38>I(+ - +)|c113>t,A38>I(+ - +)|g117>c,M39>I(+ + -)|a145>g,R49>G(- - -)|a157>g,M53>V(+ - -)|a162>g|g164>c,W55>S(- - -)|t189>c|c192>t|a197>g,K66>R(+ + +)|c213>g,F71>L(+ - -)|a235>t,T79>S(+ - +)|c257>t,T86>I(- - -)|a258>c,T86>I(- - -)|t262>a,Y88>N(- - -)|c271>t|c287>a,S96>Y(+ - -)|g303>a|c309>t| c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)|g112>a,A38>I(+ - +)|c113>t,A38>I(+ - +)| g117>c,M39>I(+ + -)|a145>g,R49>G(- - -)|a157>g,M53>V(+ - -)|a162>g|g164>c,W55>S(- - -)| t189>c|c192>t| a197>g,K66>R(+ + +)|c213>g,F71>L(+ - -)|a235>t,T79>S(+ - +)|c257>t,T86>I(- - -)|a258>c,T86>I(- - -)|t262>a,Y88>N(- - -)|c271>t|c287>a,S96>Y(+ - -)|g303>a|c309>t| 320 222 198 24 6 18 4 3 6 3 1 1 1 1 2 2 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 0 7 0 2 2 2 0 0 0 0 0 1 0 0 51 51 46 5 1 4 3 0 0 0 0 0 0 0 2 0 0 0 30 24 22 2 2 0 0 0 1 1 0 0 0 0 0 0 0 0 117 114 104 10 3 7 1 1 3 0 1 1 1 1 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|ta,262-263(FR3)|aa,290-291(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,11-12(FR1)|tt,62-63(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,120-121(FR2)|tt,149-150(FR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,201-202(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacagcttcactaatcacatt atccattgggtgcgccaggcccccggacaagggcttgagtgggtggggtcg atcaacgctggcaatggcaataca agatattcacagaagttgcagggcagagtcaccatttccagggacacatccgcgagcatcgccaacatggagttgagcagcctgagatatgaagacacggctgtatattattgt gcgagagacggctatttgcctggcaacggggctgacttt ARDGYLPGNGADF IGHV1-3 IGHD2-8 IGHJ5 ggatacagcttcactaatcacatt atccattgggtgcgccaggcccccggacaagggcttgagtgggtggggtcg atcaacgctggcaatggcaataca agatattcacagaagttgcagggcagagtcaccatttccagggacacatccgcgagcatcgccaacatggagttgagcagcctgagatatgaagacacggctgtatattattgt gcgagagacggctatttgcctggcaacggggctgacttt IGA1 24 213 16 8 9 14 7 10 4 2 11 7 JY8QFUQ01CCYZD 7124 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 892 89.95 197/219 nt 89.5 196/219 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-10*01 F 2 10 7 17 10.7.17 [1.16.38.11] CVRLDYYGSGSSYSGLDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 64 in the user submitted sequence), (cause frameshift) gtctggtggctccatcagtggtactccttactactggggctggatccgccagcccccaggtaagggctggagtggattgggcatgtatatcacagagggaccacctactacaacccgtccctcaagagtcgagtcgccatatccgtagacacgtccgagaaccggttctccctgcagttgaactctgtgaccgccgcagacacggctgtatattactgtgtgagactggattactatggttcgggaagttcttatagtggccttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 100 100 [301] c75>g|c93>t|a100>g,S34>G(+ + -)|g104>c,S35>T(+ - +)|a106>c,S36>P(+ - -)|g107>c,S36>P(+ - -)|g141>t|a163>c,S55>H(+ - -)|g164>a,S55>H(+ - -)|a166>g,I56>V(+ - +)|c168>a,I56>V(+ - +)|t172>c,Y58>H(+ - -)|t174>c,Y58>H(+ - -)|t177>a,S59>R(- - -)|g191>c,S64>T(+ - +)|a229>g,T77>A(- - -)|a250>g,K84>E(+ - -)|a257>g,Q86>R(+ - -)|a268>c,K90>Q(+ - -)|c271>t|g275>a,S92>N(- - -)|g303>a|c314>t,A105>V(+ - +)| c75>g| c93>t|a100>g,S34>G(+ + -)|g104>c,S35>T(+ - +)|a106>c,S36>P(+ - -)|g107>c,S36>P(+ - -)| g141>t|a163>c,S55>H(+ - -)|g164>a,S55>H(+ - -)| a166>g,I56>V(+ - +)|c168>a,I56>V(+ - +)|t172>c,Y58>H(+ - -)|t174>c,Y58>H(+ - -)|t177>a,S59>R(- - -)|g191>c,S64>T(+ - +)| a229>g,T77>A(- - -)|a250>g,K84>E(+ - -)|a257>g,Q86>R(+ - -)|a268>c,K90>Q(+ - -)|c271>t|g275>a,S92>N(- - -)|g303>a| c314>t,A105>V(+ - +)|a320>t| 319 (320) 226 (227) 203 23 (24) 5 (6) 18 5 3 3 2 3 1 0 (1) 1 3 1 1 0 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 30 25 5 1 4 1 0 1 0 1 0 0 0 2 0 0 0 51 50 47 3 1 2 0 1 0 0 1 0 0 0 0 0 1 0 30 21 15 6 0 6 1 0 0 2 0 1 0 1 1 0 0 0 117 114 107 7 2 5 3 2 1 0 1 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 0 1 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtct ggtggctccatcagtggtactccttactac tggggctggatccgccagcccccaggtaagggctggagtggattgggcat gtatatcacagagggaccacc tactacaacccgtccctcaagagtcgagtcgccatatccgtagacacgtccgagaaccggttctccctgcagttgaactctgtgaccgccgcagacacggctgtatattactgt gtgagactggattactatggttcgggaagttcttatagtggccttgactac VRLDYYGSGSSYSGLDY IGHV4-39 IGHD3-10 IGHJ4 ggtggctccatcagtggtactccttactac tggggctggatccgccagcccccaggtaagggctggagtggattgggcat gtatatcacagagggaccacc tactacaacccgtccctcaagagtcgagtcgccatatccgtagacacgtccgagaaccggttctccctgcagttgaactctgtgaccgccgcagacacggctgtatattactgt gtgagactggattactatggttcgggaagttcttatagtggccttgactac IGA2 21 215 12 9 5 10 7 11 3 1 7 10 JY8QFUQ01CCZ8L 11824 productive (see comment) Homsap IGHV3-7*01 F 953 93.95 202/215 nt 93.49 201/215 nt Homsap IGHJ5*02 F (see comment) 138 74.51 38/51 nt NA 8 8 9 8.8.9 [1.16.38.11] CTTTLVWFGEL (see Functionality comment) in-frame + Potentially, because of detected insertion/deletion, IMGT/JunctionAnalysis gives no results for this JUNCTION Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 56 in the user submitted sequence), (cause frameshift) tctggattcacgtttagagactattggatgagttgggtccgccaggctcctgggaggggctggagtgggtggccaacataaagcaagatgcaagtgaggaatactatgtggactctgtgaagggccggttcaccatctccagagacaacgccaagagctcactgcatttgcaaatgaacagcctgagagccgaggacacggctatgtattactgtacgaccacattagtatggttcggggagttaggccagggaacccaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [273] c87>g|t105>a,S35>R(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c120>t|a138>t|g185>c,G62>A(- + -)|a193>g,K65>E(+ - -)|a225>g|a254>g,N85>S(- - -)|t262>c,Y88>H(+ - -)|c265>t|g301>a,V101>M(+ - -)|g313>a,A105>T(- - -)| c87>g|t105>a,S35>R(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| c120>t|a138>t| g185>c,G62>A(- + -)|a193>g,K65>E(+ - -)| a225>g|a254>g,N85>S(- - -)|t262>c,Y88>H(+ - -)|c265>t|g301>a,V101>M(+ - -)| g313>a,A105>T(- - -)|g317>c,R106>T(- - -)|a318>c,R106>T(- - -)|g319>a|a320>c| 316 (320) 219 (223) 205 14 (18) 5 (7) 9 (11) 4 3 (4) 2 1 0 (2) 0 1 1 1 (2) 1 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 0 0 0 0 0 1 0 1 0 0 51 50 48 2 2 0 0 0 1 0 0 0 1 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 117 114 109 5 2 3 2 1 1 1 0 0 0 0 0 0 0 0 4 (8) 4 3 1 (5) 0 (2) 1 (3) 0 1 (2) 0 0 0 (2) 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcacgtttagagactattgg atgagttgggtccgccaggctcctgggaggggctggagtgggtggccaac ataaagcaagatgcaagtgaggaa tactatgtggactctgtgaagggccggttcaccatctccagagacaacgccaagagctcactgcatttgcaaatgaacagcctgagagccgaggacacggctatgtattactgt acgaccacattagtatggttcggggag TTTLVWFGE IGHV3-7 IGHJ5 ggattcacgtttagagactattgg atgagttgggtccgccaggctcctgggaggggctggagtgggtggccaac ataaagcaagatgcaagtgaggaa tactatgtggactctgtgaagggccggttcaccatctccagagacaacgccaagagctcactgcatttgcaaatgaacagcctgagagccgaggacacggctatgtattactgt acgaccacattagtatggttcggggag IGA1 13 212 9 4 4 6 5 7 4 1 3 5 JY8QFUQ01CD8PO 6781 productive (see comment) Homsap IGHV2-5*02 F 1165 97.94 238/243 nt 97.53 237/243 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD5-12*01 F 2 10 7 11 10.7.11 [9.16.38.11] CAHRRIRFGFDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 45 of V-REGION: 1 nucleotides (from position 79 in the user submitted sequence), (cause frameshift) gaccctcacgctgacctgcaccttctctgggttctcactcagcactggtggagtgggtgtgggctggatccgtcagccccaggaaaggccctggagtggcttgcactcatttattgggatgatgataagcgctacagcccatctctgaagagcagactcaccatcaccaaggacacgtccaagaaccaggtggtcctcacaatgaccaacatggaccctgtggacacagccacatattactgtgcacacaggaggatacgattcggctttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggctcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG2 100 94 [307, 308] a103>g,S35>G(+ + -)|g225>a|c246>g|a252>g|t267>c| a103>g,S35>G(+ + -)| g225>a|c246>g|a252>g|t267>c| a321>g|c322>a| 320 (322) 251 (253) 246 5 (7) 4 (6) 1 2 (3) 1 0 1 0 0 (1) 0 0 0 1 0 0 78 28 28 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 29 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 50 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 110 4 4 0 1 1 0 1 0 0 0 0 0 1 0 0 8 (10) 8 8 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 0 aa,13-14(FR1)|aa,40-41(FR1)|ta,102-103(CDR1)|aa,141-142(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,238-239(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,267-268(FR3)|aa,270-271(FR3)|aa,277-278(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,10-11(FR1)|tt,73-74(FR1)|tt,82-83(CDR1)|ta,102-103(CDR1)|tt,158-159(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,80-83(CDR1)|ggct,118-121(FR2)|ggct,155-158(FR2)|agca,220-223(FR3)|ggct,224-227(FR3)| aacc,41-44(FR1)|agcc,131-134(FR2)|agcc,202-205(FR3)|aacc,253-256(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)| gaccctcacgctgacctgcaccttctct gggttctcactcagcactggtggagtgggt gtgggctggatccgtcagccccaggaaaggccctggagtggcttgcactc atttattgggatgatgataag cgctacagcccatctctgaagagcagactcaccatcaccaaggacacgtccaagaaccaggtggtcctcacaatgaccaacatggaccctgtggacacagccacatattactgt gcacacaggaggatacgattcggctttgactac AHRRIRFGFDY IGHV2-5 IGHD5-12 IGHJ4 gggttctcactcagcactggtggagtgggt gtgggctggatccgtcagccccaggaaaggccctggagtggcttgcactc atttattgggatgatgataag cgctacagcccatctctgaagagcagactcaccatcaccaaggacacgtccaagaaccaggtggtcctcacaatgaccaacatggaccctgtggacacagccacatattactgt gcacacaggaggatacgattcggctttgactac IGG2 5 215 4 1 1 2 3 3 4 0 0 1 JY8QFUQ01CDA18 11071 productive Homsap IGHV4-61*02 F 830 86.43 191/221 nt NA Homsap IGHJ6*02 F 229 85.48 53/62 nt Homsap IGHD2-21*01 F 2 10 7 19 10.7.19 [1.17.38.11] CAREAPKTAGDSYGSLAMDVW in-frame + tctctggtggctccgtcagtagtggttcttactactggagctggacccggcagcccgccgggaagggactggagtggattgggcgtgtccacagcggtggcagttccgactacaacccgtccctcacgggtcgagccaccatcttagtggactcctccaagaatcagttctccctgaggctgacctctgtgacggccgcagacgcggccgagtatttctgtgcgagagaggcgcccaagacagcaggtgattcctacggctctctcgctatggacgtctggggccaagggaccacggtcatcgtctcctcagcatcccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccaggcttcttcccccc NA IGA1 100 95 [310] a88>g,I30>V(+ - +)|c93>t|a106>t|g107>c|t125>c,I42>T(- - -)|a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|t171>c,Y57>H(+ - -)|c173>g,T58>S(+ - +)|a175>g,S59>G(+ + -)|g189>c|c192>t|a193>t,T65>S(+ - +)|a196>g,N66>D(+ + -)|c207>g|a215>c,K72>T(- - -)|a220>g,S74>G(+ + -)|t227>c,V76>A(+ - +)|a234>c|c236>t,S79>L(- - -)|a240>g|a244>t,T82>S(+ - +)|g246>c,T82>S(+ - +)|c255>t|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c285>g|a295>g,T99>A(- - -)|t302>a,V101>E(- + -)|a308>t,Y103>F(- + -)| a88>g,I30>V(+ - +)|c93>t|a106>t|g107>c| t125>c,I42>T(- - -)| a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|t171>c,Y57>H(+ - -)|c173>g,T58>S(+ - +)|a175>g,S59>G(+ + -)|g189>c|c192>t|a193>t,T65>S(+ - +)| a196>g,N66>D(+ + -)|c207>g|a215>c,K72>T(- - -)|a220>g,S74>G(+ + -)|t227>c,V76>A(+ - +)|a234>c|c236>t,S79>L(- - -)|a240>g|a244>t,T82>S(+ - +)|g246>c,T82>S(+ - +)|c255>t|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c285>g|a295>g,T99>A(- - -)|t302>a,V101>E(- + -)|a308>t,Y103>F(- + -)| 320 229 199 30 10 20 8 0 4 4 2 0 4 1 4 3 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 3 1 1 0 1 0 0 0 1 0 1 0 0 0 51 51 50 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 30 21 13 8 2 6 2 0 1 2 0 0 1 0 1 1 0 0 117 114 97 17 5 12 5 0 2 1 2 0 2 1 2 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcagtagtggttcttactac tggagctggacccggcagcccgccgggaagggactggagtggattgggcgt gtccacagcggtggcagttcc gactacaacccgtccctcacgggtcgagccaccatcttagtggactcctccaagaatcagttctccctgaggctgacctctgtgacggccgcagacgcggccgagtatttctgt gcgagagaggcgcccaagacagcaggtgattcctacggctctctcgctatggacgtc AREAPKTAGDSYGSLAMDV IGHV4-61 IGHD2-21 IGHJ6 ggtggctccgtcagtagtggttcttactac tggagctggacccggcagcccgccgggaagggactggagtggattgggcgt gtccacagcggtggcagttcc gactacaacccgtccctcacgggtcgagccaccatcttagtggactcctccaagaatcagttctccctgaggctgacctctgtgacggccgcagacgcggccgagtatttctgt gcgagagaggcgcccaagacagcaggtgattcctacggctctctcgctatggacgtc IGA1 30 216 16 14 4 11 12 19 5 5 13 7 JY8QFUQ01CDA2Z 20264 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 842 87.61 191/218 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD2-8*01 F 1 8 8 13 8.8.13 [1.17.38.11] CAKDRIGSLYYFDHW in-frame - cctccggattcacctttagtagtttctggatgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacggactaatgagtatgggagtatcacaaactacgcggactccgtggagggccgattcaccatctccagagacaacagcaaaaactccctgtatctgcaaatgaacagtctgagaactgaggacaccgccttgtattactgtgcaaaagataggattgggtccttgtactactttgaccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [288] t78>c|c90>t|c108>t|a110>t,Y37>F(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g247>a,A83>S(- + -)|c248>g,A83>S(- + -)|g252>a|a256>t,T86>S(+ - +)|g258>c,T86>S(+ - +)|g286>a,A96>T(- - -)|c288>t,A96>T(- - -)|g297>c|t300>c|g301>t,V101>L(+ - +)|g317>a,R106>K(+ + +)| t78>c| c90>t|c108>t|a110>t,Y37>F(- + -)| c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g247>a,A83>S(- + -)|c248>g,A83>S(- + -)|g252>a|a256>t,T86>S(+ - +)|g258>c,T86>S(+ - +)|g286>a,A96>T(- - -)|c288>t,A96>T(- - -)|g297>c|t300>c|g301>t,V101>L(+ - +)| g317>a,R106>K(+ + +)| 320 226 198 28 8 20 3 7 4 3 0 0 2 0 2 1 3 3 78 5 4 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 2 0 0 0 1 0 0 0 0 0 51 51 46 5 2 3 1 1 1 0 0 0 0 0 0 0 0 2 30 24 18 6 0 6 1 1 0 1 0 0 0 0 0 0 2 1 117 114 102 12 3 9 1 4 1 1 0 0 1 0 2 1 1 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctcc ggattcacctttagtagtttctgg atgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggagggccgattcaccatctccagagacaacagcaaaaactccctgtatctgcaaatgaacagtctgagaactgaggacaccgccttgtattactgt gcaaaagataggattgggtccttgtactactttgaccac AKDRIGSLYYFDH IGHV3-74 IGHD2-8 IGHJ4 ggattcacctttagtagtttctgg atgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggagggccgattcaccatctccagagacaacagcaaaaactccctgtatctgcaaatgaacagtctgagaactgaggacaccgccttgtattactgt gcaaaagataggattgggtccttgtactactttgaccac IGA1 26 213 15 11 10 16 5 10 5 2 12 7 JY8QFUQ01CDCEV 21743 productive (see comment) Homsap IGHV3-66*02 F 898 91.55 195/213 nt 90.61 193/213 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-15*01 F 3 8 7 16 8.7.16 [1.16.38.11] CAKLADTGEWSNRIFYFW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 52 of V-REGION: 'A' (1 nucleotides at position 68 to 68 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 44 of V-REGION: 1 nucleotides (from position 46 in the user submitted sequence), (cause frameshift) ctctggattcaccgtcagtagcagcttcatgacttgggtccgccagctccaggaaagggactggagtAgggtctcagtgctttatgtcggtggtaacacatactacgcagactccgtgaagggccgattcaccacctccagagacaattccgagaacactctgtatcttcaaatgaacaacctgagacctgaggactcggctgtgtattattgtgcgaaattggcggatactggagagtggagcaaccgcatcttttatttctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcagg NA IGG2 100 100 [294] a110>g,N37>S(- - -)|a113>t,Y38>F(- + -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g141>a|g147>a|t165>g|a166>c,I56>L(+ + +)|a172>g,S58>V(- - -)|g173>t,S58>V(- - -)|g191>a,S64>N(- - -)|t233>c,I78>T(- - -)|a250>g,K84>E(+ - -)|g258>t|g278>a,S93>N(- - -)|g286>c,A96>P(- - -)|a295>t,T99>S(+ - +)|c309>t| a110>g,N37>S(- - -)|a113>t,Y38>F(- + -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g141>a|g147>a|t165>g| a166>c,I56>L(+ + +)|a172>g,S58>V(- - -)|g173>t,S58>V(- - -)|g191>a,S64>N(- - -)| t233>c,I78>T(- - -)|a250>g,K84>E(+ - -)|g258>t|g278>a,S93>N(- - -)|g286>c,A96>P(- - -)|a295>t,T99>S(+ - +)|c309>t| g317>a,R106>K(+ + +)| 316 (318) 217 (219) 199 (200) 18 (19) 5 13 (14) 3 4 (5) 2 1 1 0 2 0 2 0 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 51 50 45 5 3 2 0 2 1 0 0 0 0 0 1 0 0 1 30 21 17 4 0 4 1 1 0 0 1 0 0 0 0 0 1 0 117 114 107 7 2 5 1 1 1 1 0 0 1 0 1 0 1 0 4 (6) 4 4 (5) 0 (1) 0 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccgtcagtagcagcttc atgacttgggtccgccagctccaggaaagggactggagtgggtctcagtg ctttatgtcggtggtaacaca tactacgcagactccgtgaagggccgattcaccacctccagagacaattccgagaacactctgtatcttcaaatgaacaacctgagacctgaggactcggctgtgtattattgt gcgaaattggcggatactggagagtggagcaaccgcatcttttatttc AKLADTGEWSNRIFYF IGHV3-66 IGHD2-15 IGHJ4 ggattcaccgtcagtagcagcttc atgacttgggtccgccagctccaggaaagggactggagtgggtctcagtg ctttatgtcggtggtaacaca tactacgcagactccgtgaagggccgattcaccacctccagagacaattccgagaacactctgtatcttcaaatgaacaacctgagacctgaggactcggctgtgtattattgt gcgaaattggcggatactggagagtggagcaaccgcatcttttatttc IGG2 18 209 10 8 6 10 4 8 5 0 7 6 JY8QFUQ01CDHKA 20556 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 928 91.32 200/219 nt 90.41 198/219 nt Homsap IGHJ1*01 F (see comment) 142 76 38/50 nt Homsap IGHD5-18*01 F 3 8 8 6 8.8.6 [2.17.38.11] CASYGQWL in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 48 of V-REGION: 'G' (1 nucleotides at position 60 to 60 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 64 of V-REGION: 'ATTT' (4 nucleotides at position 102 to 105 in the user submitted sequence), (cause frameshift); gcctcgggactcatgtttagcagctatgccatgacctgggtccgccaggctccagggaaGggggctggagtgggtctcaagtgtcagtagtagtactggttATTTtcacatactacacagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtatattattgcgctagttacgggcagtggctgggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcagg NA IGG2 100 100 [274] t78>g|t82>c,F28>L(+ - -)|c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|g119>c,S40>T(+ - +)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g172>a,G58>S(+ + -)|g184>a,G62>T(+ - -)|g185>c,G62>T(+ - -)|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)|g202>a,A68>T(- - -)|a275>g,N92>S(- - -)|g291>a|c309>t|t312>c| t78>g| t82>c,F28>L(+ - -)|c86>t,T29>M(- - -)|c87>g,T29>M(- - -)| g119>c,S40>T(+ - +)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g172>a,G58>S(+ + -)|g184>a,G62>T(+ - -)|g185>c,G62>T(+ - -)|a190>t,S64>F(- - -)|g191>t,S64>F(- - -)| g202>a,A68>T(- - -)|a275>g,N92>S(- - -)|g291>a|c309>t|t312>c| g315>t|a317>g,K106>S(- - -)|a318>t,K106>S(- - -)|g319>t| 314 (320) 221 (227) 202 (204) 19 (23) 4 (6) 15 (17) 2 (3) 5 2 3 0 0 1 (2) 0 2 2 1 (3) 1 78 6 5 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 36 24 21 3 0 3 0 0 1 1 0 0 0 0 0 1 0 0 51 51 48 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 30 24 17 7 0 7 1 2 0 1 0 0 1 0 1 0 1 0 117 114 109 5 3 2 1 2 1 1 0 0 0 0 0 0 0 0 2 (8) 2 2 (4) 0 (4) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (2) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctcg ggactcatgtttagcagctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcaagt gtcagtagtagtactggtttcaca tactacacagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtatattattgc gctagttacgggcagtgg ASYGQW IGHV3-23 IGHD5-18 IGHJ1 ggactcatgtttagcagctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcaagt gtcagtagtagtactggtttcaca tactacacagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgagcagcctgagagccgaagacacggccgtatattattgc gctagttacgggcagtgg IGG2 18 213 12 6 7 12 5 6 3 0 5 10 JY8QFUQ01CDYHQ 17761 productive Homsap IGHV3-66*02 F 908 91.63 197/215 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-15*01 F 3 8 7 16 8.7.16 [1.17.38.11] CAKLADTGEWSNRIFYFW in-frame + cctctggattcaccgtcagtagcagcttcatgacttgggtccgccaggctccaggaaagggactggagtgggtctcagtgctttatgtcggtggtaacacatactacgcagactccgtgaagggccgattcaccacctccagagacaattccgagaacactctgtatcttcaaatgaacaacctgagacctgaggactcggctgtgtattattgtgcgaaattggcggatactggagagtggagcaaccgcatcttttatttctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcctcctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 88 [295] a110>g,N37>S(- - -)|a113>t,Y38>F(- + -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g141>a|g147>a|t165>g|a166>c,I56>L(+ + +)|a172>g,S58>V(- - -)|g173>t,S58>V(- - -)|g191>a,S64>N(- - -)|t233>c,I78>T(- - -)|a250>g,K84>E(+ - -)|g258>t|g278>a,S93>N(- - -)|g286>c,A96>P(- - -)|a295>t,T99>S(+ - +)|c309>t| a110>g,N37>S(- - -)|a113>t,Y38>F(- + -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g141>a|g147>a|t165>g| a166>c,I56>L(+ + +)|a172>g,S58>V(- - -)|g173>t,S58>V(- - -)|g191>a,S64>N(- - -)| t233>c,I78>T(- - -)|a250>g,K84>E(+ - -)|g258>t|g278>a,S93>N(- - -)|g286>c,A96>P(- - -)|a295>t,T99>S(+ - +)|c309>t| g317>a,R106>K(+ + +)| 316 (318) 219 (221) 201 (202) 18 (19) 5 13 (14) 3 4 (5) 2 1 1 0 2 0 2 0 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 51 51 46 5 3 2 0 2 1 0 0 0 0 0 1 0 0 1 30 21 17 4 0 4 1 1 0 0 1 0 0 0 0 0 1 0 117 114 107 7 2 5 1 1 1 1 0 0 1 0 1 0 1 0 4 (6) 4 4 (5) 0 (1) 0 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccgtcagtagcagcttc atgacttgggtccgccaggctccaggaaagggactggagtgggtctcagtg ctttatgtcggtggtaacaca tactacgcagactccgtgaagggccgattcaccacctccagagacaattccgagaacactctgtatcttcaaatgaacaacctgagacctgaggactcggctgtgtattattgt gcgaaattggcggatactggagagtggagcaaccgcatcttttatttc AKLADTGEWSNRIFYF IGHV3-66 IGHD2-15 IGHJ4 ggattcaccgtcagtagcagcttc atgacttgggtccgccaggctccaggaaagggactggagtgggtctcagtg ctttatgtcggtggtaacaca tactacgcagactccgtgaagggccgattcaccacctccagagacaattccgagaacactctgtatcttcaaatgaacaacctgagacctgaggactcggctgtgtattattgt gcgaaattggcggatactggagagtggagcaaccgcatcttttatttc IGG1 18 210 10 8 6 10 4 8 5 0 7 6 JY8QFUQ01CE2OJ 16402 productive Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F or Homsap IGHV4-34*12 F (see comment) 781 84.98 181/213 nt NA Homsap IGHJ5*02 F 192 86.27 44/51 nt Homsap IGHD2-15*01 F 2 8 7 18 8.7.18 [1.17.38.11] CARGEECRSGSCYSVWFDPW in-frame + Low V-REGION identity (84,98% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tctggtgggtccttaagtgactcctactgggcctggatccgccagcccccagggaagggcctggagtacattggggagatcagtcatgatggtagaaccatgttcaattggtccctcaagagtcgactcaccatctcagtagacacgtccaagaatcaattctccctgagattgacctctgtgaccgccgcggacacggctgtttattactgcgcgagaggcgaggaatgtcggagtggaagttgctactccgtttggttcgacccctggggccagggagcccaagtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [298] a77>c,Y26>S(+ - -)|c90>a,F30>L(+ - -)|g107>a,G36>D(- - -)|t108>c,G36>D(- - -)|a110>c,Y37>S(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g147>c|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|a165>g|a170>g,N57>S(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|a189>t|c192>a,S64>R(- - -)|a197>t,N66>M(- - -)|c198>g,N66>M(- - -)|a200>t,Y67>F(- + -)|c204>t|c205>t,P69>W(- - -)|c206>g,P69>W(- - -)|g226>c,V76>L(+ - +)|a234>c|c255>t|g258>a|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c271>t|g275>c,S92>T(+ - +)|g303>t|t312>c| a77>c,Y26>S(+ - -)| c90>a,F30>L(+ - -)|g107>a,G36>D(- - -)|t108>c,G36>D(- - -)|a110>c,Y37>S(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g147>c|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|a165>g| a170>g,N57>S(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|a189>t|c192>a,S64>R(- - -)| a197>t,N66>M(- - -)|c198>g,N66>M(- - -)|a200>t,Y67>F(- + -)|c204>t|c205>t,P69>W(- - -)|c206>g,P69>W(- - -)|g226>c,V76>L(+ - +)|a234>c|c255>t|g258>a|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c271>t|g275>c,S92>T(+ - +)|g303>t|t312>c| 320 221 189 32 10 22 5 5 4 2 3 2 3 0 5 2 1 0 78 3 2 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 0 1 1 1 0 0 0 0 0 0 51 51 45 6 2 4 2 1 0 0 0 0 0 0 3 0 0 0 30 21 16 5 1 4 2 1 0 0 0 1 1 0 0 0 0 0 117 114 98 16 7 9 1 2 4 1 1 0 2 0 2 2 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tct ggtgggtccttaagtgactcctac tgggcctggatccgccagcccccagggaagggcctggagtacattggggag atcagtcatgatggtagaacc atgttcaattggtccctcaagagtcgactcaccatctcagtagacacgtccaagaatcaattctccctgagattgacctctgtgaccgccgcggacacggctgtttattactgc gcgagaggcgaggaatgtcggagtggaagttgctactccgtttggttcgacccc ARGEECRSGSCYSVWFDP IGHV4-34 IGHD2-15 IGHJ5 ggtgggtccttaagtgactcctac tgggcctggatccgccagcccccagggaagggcctggagtacattggggag atcagtcatgatggtagaacc atgttcaattggtccctcaagagtcgactcaccatctcagtagacacgtccaagaatcaattctccctgagattgacctctgtgaccgccgcggacacggctgtttattactgc gcgagaggcgaggaatgtcggagtggaagttgctactccgtttggttcgacccc IGA1 31 210 16 15 9 19 7 12 9 1 13 8 JY8QFUQ01CE4MI 11480 productive (see comment) Homsap IGHV3-73*01 F, or Homsap IGHV3-73*02 F 1105 100 222/222 nt 99.55 221/222 nt Homsap IGHJ6*02 F 283 95.16 59/62 nt Homsap IGHD6-6*01 F 2 8 10 16 8.10.16 [1.17.38.11] CTRRSIADNFYYYGMDVW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 72 of V-REGION: 'A' (1 nucleotides at position 127 to 127 in the user submitted sequence), (cause frameshift); tctgggttcaccttcagtggctctgctatgcactgggtccgccaggcttccgggaaagggctggagtgggttggccgtattagaagcaaagctaacagttacgcgacagcatatgctgcgtcggtgAaaaggcaggttcaccatctccagagatgattcaaagaacacggcgtatctgcaaatgaacagcctgaaaaccgaggacacggccgtgtattactgtactagacggagtatagcagataacttctactactacggtatggacgtctggggccaagggaccacagtcaccgtctcctcagcttccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG3 100 100 [303] a320>g| 319 (320) 229 (230) 229 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,58-59(FR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,114-115(CDR1)|tt,135-136(FR2)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|ggct,106-109(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|agca,195-198(CDR2)|ggca,220-223(FR3)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,59-62(FR1)|agcc,72-75(FR1)|tgct,111-114(CDR1)|agct,177-180(CDR2)|tgct,201-204(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| tct gggttcaccttcagtggctctgct atgcactgggtccgccaggcttccgggaaagggctggagtgggttggccgt attagaagcaaagctaacagttacgcgaca gcatatgctgcgtcggtgaaaggcaggttcaccatctccagagatgattcaaagaacacggcgtatctgcaaatgaacagcctgaaaaccgaggacacggccgtgtattactgt actagacggagtatagcagataacttctactactacggtatggacgtc TRRSIADNFYYYGMDV IGHV3-73 IGHD6-6 IGHJ6 gggttcaccttcagtggctctgct atgcactgggtccgccaggcttccgggaaagggctggagtgggttggccgt attagaagcaaagctaacagttacgcgaca gcatatgctgcgtcggtgaaaggcaggttcaccatctccagagatgattcaaagaacacggcgtatctgcaaatgaacagcctgaaaaccgaggacacggccgtgtattactgt actagacggagtatagcagataacttctactactacggtatggacgtc IGG3 0 219 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01CEBF1 11439 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 954 93.55 203/217 nt NA Homsap IGHJ2*01 F 220 90.57 48/53 nt Homsap IGHD1-26*01 F 3 8 8 12 8.8.12 [1.17.38.11] CARPFSGRWCFDLW in-frame + ctctggattcaccttcagttactcctggatgcactgggtccgccaagttccaggaaaggggccggtgtgggtctcacaaattaaaagtgatgggagtaccccaagttacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagtcgaggacacggctgtttattactgtgcaagaccgtttagtgggaggtggtgcttcgatctctggggccgtggcaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a110>c,Y37>S(+ - -)|c134>t,A45>V(+ - +)|g141>a|t149>c,L50>P(- - -)|g164>a,R55>Q(+ - -)|t165>a,R55>Q(+ - -)|t171>a,N57>K(+ - -)|g191>c,S64>T(+ - +)|a193>c,T65>P(+ + -)|c198>t|c287>t,A96>V(+ - +)|g303>t| a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a110>c,Y37>S(+ - -)| c134>t,A45>V(+ - +)|g141>a|t149>c,L50>P(- - -)|g164>a,R55>Q(+ - -)|t165>a,R55>Q(+ - -)| t171>a,N57>K(+ - -)|g191>c,S64>T(+ - +)|a193>c,T65>P(+ + -)| c198>t|c287>t,A96>V(+ - +)|g303>t| g319>c|a320>c| 318 (320) 223 (225) 209 14 (16) 3 (5) 11 0 3 3 1 2 (3) 0 1 2 1 (2) 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 1 0 1 0 0 0 0 0 51 51 46 5 1 4 0 2 1 1 0 0 0 1 0 0 0 0 30 24 21 3 0 3 0 0 0 0 1 0 0 1 1 0 0 0 117 114 111 3 2 1 0 0 2 0 0 0 0 0 0 0 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagttactcctgg atgcactgggtccgccaagttccaggaaaggggccggtgtgggtctcacaa attaaaagtgatgggagtacccca agttacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagtcgaggacacggctgtttattactgt gcaagaccgtttagtgggaggtggtgcttcgatctc ARPFSGRWCFDL IGHV3-74 IGHD1-26 IGHJ2 ggattcaccttcagttactcctgg atgcactgggtccgccaagttccaggaaaggggccggtgtgggtctcacaa attaaaagtgatgggagtacccca agttacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagtcgaggacacggctgtttattactgt gcaagaccgtttagtgggaggtggtgcttcgatctc IGA1 14 213 7 7 6 8 1 6 3 0 5 6 JY8QFUQ01CEEUJ 14875 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*05 F or Homsap IGHV3-33*06 F 846 88.02 191/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-22*01 F 2 8 8 15 8.8.15 [1.17.38.11] CVRDDDTSGHYSLFKYW in-frame + gtctggattctcctttagtgcatatggcatacactgggtccgccagactccaggcaaggggctggagtgggtggctgttatgtattttgatggagttagaacattttatgcagactccgtgaagggccgattcaccctctccaaagactattccaagaacacggtgcatctgcaaatgaacagcctgcgagccgaggacacggctgtatattactgtgtgagagatgatgataccagtggtcactactctctgtttaaatattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [293] a85>t,T29>S(+ - +)|c90>t|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|c108>a,S36>A(- + -)|g117>a,M39>I(+ + -)|g133>a,A45>T(- - -)|a162>t|a168>g,I56>M(+ + -)|g170>a,W57>Y(- + -)|g171>t,W57>Y(- + -)|a173>t,Y58>F(- + -)|a187>g,S63>V(- - -)|g188>t,S63>V(- - -)|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)|a194>c,K65>T(- - -)|a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|a232>c,I78>L(+ + +)|g239>a,R80>K(+ + +)|a244>t,N82>Y(- - -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)|a283>c|g303>a|c314>t,A105>V(+ - +)| a85>t,T29>S(+ - +)|c90>t|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|c108>a,S36>A(- + -)| g117>a,M39>I(+ + -)|g133>a,A45>T(- - -)|a162>t| a168>g,I56>M(+ + -)|g170>a,W57>Y(- + -)|g171>t,W57>Y(- + -)|a173>t,Y58>F(- + -)|a187>g,S63>V(- - -)|g188>t,S63>V(- - -)|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)|a194>c,K65>T(- - -)| a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|a232>c,I78>L(+ + +)|g239>a,R80>K(+ + +)|a244>t,N82>Y(- - -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)|a283>c|g303>a| c314>t,A105>V(+ - +)| 320 225 198 27 4 23 4 5 3 1 3 1 5 1 1 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 1 0 1 0 0 1 1 0 1 0 0 0 51 51 48 3 1 2 0 2 0 0 0 0 1 0 0 0 0 0 30 24 15 9 0 9 3 1 0 0 1 0 1 1 0 0 2 0 117 114 105 9 2 7 0 2 1 1 2 0 2 0 0 1 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattctcctttagtgcatatggc atacactgggtccgccagactccaggcaaggggctggagtgggtggctgtt atgtattttgatggagttagaaca ttttatgcagactccgtgaagggccgattcaccctctccaaagactattccaagaacacggtgcatctgcaaatgaacagcctgcgagccgaggacacggctgtatattactgt gtgagagatgatgataccagtggtcactactctctgtttaaatat VRDDDTSGHYSLFKY IGHV3-33 IGHD3-22 IGHJ4 ggattctcctttagtgcatatggc atacactgggtccgccagactccaggcaaggggctggagtgggtggctgtt atgtattttgatggagttagaaca ttttatgcagactccgtgaagggccgattcaccctctccaaagactattccaagaacacggtgcatctgcaaatgaacagcctgcgagccgaggacacggctgtatattactgt gtgagagatgatgataccagtggtcactactctctgtttaaatat IGA2 26 213 12 14 7 12 5 14 3 1 9 13 JY8QFUQ01CEH7I 10608 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 918 91.24 198/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD5-12*01 F 3 8 8 13 8.8.13 [1.17.38.11] CAKGRGYNDYGLDYW in-frame + ctctggattcaccttcagtgactatggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtgacagttattttatatgatggaagtagaaaatactatgcagactccgtgaagggccgattcgccatctccagagacgtttcgaggaacacgttgtatctgcagatgaatagcctgagacctgaggacacggctgtatactactgcgcgaaaggacgtggatataatgactacggcctcgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [288] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g160>a,A54>T(- - -)|a168>t|c170>t,S57>L(- - -)|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)|a229>g,T77>A(- - -)|a244>g,N82>V(- - -)|a245>t,N82>V(- - -)|c249>g|a251>g,K84>R(+ + +)|c259>t|a270>g|c276>t|g286>c,A96>P(- - -)|g303>a|t306>c|t312>c|g317>a,R106>K(+ + +)| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g160>a,A54>T(- - -)| a168>t|c170>t,S57>L(- - -)|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)| a229>g,T77>A(- - -)|a244>g,N82>V(- - -)|a245>t,N82>V(- - -)|c249>g|a251>g,K84>R(+ + +)|c259>t|a270>g|c276>t|g286>c,A96>P(- - -)|g303>a|t306>c|t312>c| g317>a,R106>K(+ + +)|a320>g| 319 (320) 224 (225) 204 20 (21) 8 (9) 12 6 (7) 4 3 2 0 0 2 1 1 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 1 0 1 0 0 0 1 1 0 0 0 0 117 114 102 12 7 5 4 1 2 2 0 0 1 0 1 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgactatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtgacagtt attttatatgatggaagtagaaaa tactatgcagactccgtgaagggccgattcgccatctccagagacgtttcgaggaacacgttgtatctgcagatgaatagcctgagacctgaggacacggctgtatactactgc gcgaaaggacgtggatataatgactacggcctcgactac AKGRGYNDYGLDY IGHV3-30 IGHD5-12 IGHJ4 ggattcaccttcagtgactatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtgacagtt attttatatgatggaagtagaaaa tactatgcagactccgtgaagggccgattcgccatctccagagacgtttcgaggaacacgttgtatctgcagatgaatagcctgagacctgaggacacggctgtatactactgc gcgaaaggacgtggatataatgactacggcctcgactac IGG1 19 213 14 5 6 8 8 11 7 1 6 5 JY8QFUQ01CF2KP 13212 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 855 88.48 192/217 nt NA Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD3-22*01 F 3 8 8 15 8.8.15 [1.17.38.11] CARRSNPNAMMAAFDIW in-frame + ttctggattcagtcctaccgatttttggatcggctgggttcgccagctgcccggcaaaggcctggagtggatgggcctcatttatcctggtgactctgagaccagattcaacccgtccttccaaggccaggtcaccatctcagccaacaagtccataaataccgcctacctacagtggagcagcctgaaggcctcggacactgccgtgtattactgtgcgaggcgatccaaccctaatgctatgatggcggcgtttgatatctggggccaagggacaatggtcaccgtttcttcagcctccaccaaggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 88 [293] a83>t,Y28>F(- + -)|c87>t|t88>c,F30>P(- - -)|t89>c,F30>P(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|a110>t,Y37>F(- + -)|c111>t,Y37>F(- + -)|g126>t|a133>c,M45>L(+ + -)|g141>c|g162>c|a163>c,I55>L(+ + +)|c168>t|t192>g,D64>E(+ - +)|a200>t,Y67>F(- + -)|g203>a,S68>N(- - -)|g241>a,D81>N(+ + -)|c252>a|g254>a,S85>N(- - -)|c255>t,S85>N(- - -)|g267>a|c297>t|a301>g,M101>V(+ - -)| a83>t,Y28>F(- + -)|c87>t|t88>c,F30>P(- - -)|t89>c,F30>P(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|a110>t,Y37>F(- + -)|c111>t,Y37>F(- + -)| g126>t|a133>c,M45>L(+ + -)|g141>c|g162>c|a163>c,I55>L(+ + +)| c168>t|t192>g,D64>E(+ - +)| a200>t,Y67>F(- + -)|g203>a,S68>N(- - -)|g241>a,D81>N(+ + -)|c252>a|g254>a,S85>N(- - -)|c255>t,S85>N(- - -)|g267>a|c297>t|a301>g,M101>V(+ - -)| a318>g|a320>g| 317 (320) 222 (225) 197 (198) 25 (27) 8 (10) 17 2 (4) 5 6 2 2 1 3 0 2 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 15 9 1 8 1 1 3 2 0 0 2 0 0 0 0 0 51 51 46 5 3 2 0 0 0 0 2 0 0 0 2 0 1 0 30 24 22 2 1 1 0 0 1 0 0 0 0 0 0 0 0 1 117 114 105 9 3 6 1 4 2 0 0 1 1 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ttct ggattcagtcctaccgatttttgg atcggctgggttcgccagctgcccggcaaaggcctggagtggatgggcctc atttatcctggtgactctgagacc agattcaacccgtccttccaaggccaggtcaccatctcagccaacaagtccataaataccgcctacctacagtggagcagcctgaaggcctcggacactgccgtgtattactgt gcgaggcgatccaaccctaatgctatgatggcggcgtttgatatc ARRSNPNAMMAAFDI IGHV5-51 IGHD3-22 IGHJ3 ggattcagtcctaccgatttttgg atcggctgggttcgccagctgcccggcaaaggcctggagtggatgggcctc atttatcctggtgactctgagacc agattcaacccgtccttccaaggccaggtcaccatctcagccaacaagtccataaataccgcctacctacagtggagcagcctgaaggcctcggacactgccgtgtattactgt gcgaggcgatccaaccctaatgctatgatggcggcgtttgatatc IGG1 25 213 15 10 11 15 4 10 6 2 8 9 JY8QFUQ01CF6ST 9960 productive Homsap IGHV3-7*01 F 828 87.1 189/217 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 147 76.47 39/51 nt Homsap IGHD5-12*01 F 3 8 8 13 8.8.13 [1.17.38.11] CGRDERGADLPNDSW in-frame - Other possibilities: Homsap_IGHJ5*02 and Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctggattcaccgttagtaggtattggatgagctgggtccgccagtctccagggaagggactggagtggctggcccacataggaggagatggaagtgaggctggttatgtggactctgtgaggggccgattcttcatctccagagacaacgccaagagctccctctatctgcagatgaacagcctgagccccgaggacacgggtgtgtattattgtggcagagatgaaaggggcgccgacttgccgaatgattcctggggcctgggaaccctggtcaccgtctcctcaccatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [287] t88>g,F30>V(+ - -)|c108>g,S36>R(- - -)|g133>t,A45>S(- + -)|g147>a|g157>c,V53>L(+ - +)|a163>c,N55>H(- - -)|a169>g,K57>G(- - -)|a170>g,K57>G(- - -)|g171>a,K57>G(- - -)|c172>g,Q58>G(- - -)|a173>g,Q58>G(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)|a195>t,K65>A(- - -)|t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>t,Y66>G(+ - -)|a215>g,K72>R(+ + +)|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|a254>g,N85>S(- - -)|a258>c|g261>c|a270>g|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|c299>g,A100>G(- + -)|c309>t|c314>g,A105>G(- + -)|g315>c,A105>G(- + -)| t88>g,F30>V(+ - -)|c108>g,S36>R(- - -)| g133>t,A45>S(- + -)|g147>a|g157>c,V53>L(+ - +)|a163>c,N55>H(- - -)| a169>g,K57>G(- - -)|a170>g,K57>G(- - -)|g171>a,K57>G(- - -)|c172>g,Q58>G(- - -)|a173>g,Q58>G(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)|a195>t,K65>A(- - -)| t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>t,Y66>G(+ - -)|a215>g,K72>R(+ + +)|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|a254>g,N85>S(- - -)|a258>c|g261>c|a270>g|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|c299>g,A100>G(- + -)|c309>t| c314>g,A105>G(- + -)|g315>c,A105>G(- + -)| 320 225 195 30 5 25 8 2 3 0 4 0 2 0 4 4 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 0 0 0 1 0 1 51 51 47 4 1 3 0 1 0 0 1 0 0 0 1 0 1 0 30 24 16 8 0 8 4 1 0 0 1 0 1 0 0 1 0 0 117 114 100 14 4 10 4 0 3 0 2 0 1 0 2 1 0 1 8 8 6 2 0 2 0 0 0 0 0 0 0 0 1 1 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccgttagtaggtattgg atgagctgggtccgccagtctccagggaagggactggagtggctggcccac ataggaggagatggaagtgaggct ggttatgtggactctgtgaggggccgattcttcatctccagagacaacgccaagagctccctctatctgcagatgaacagcctgagccccgaggacacgggtgtgtattattgt ggcagagatgaaaggggcgccgacttgccgaatgattcc GRDERGADLPNDS IGHV3-7 IGHD5-12 IGHJ5 ggattcaccgttagtaggtattgg atgagctgggtccgccagtctccagggaagggactggagtggctggcccac ataggaggagatggaagtgaggct ggttatgtggactctgtgaggggccgattcttcatctccagagacaacgccaagagctccctctatctgcagatgaacagcctgagccccgaggacacgggtgtgtattattgt ggcagagatgaaaggggcgccgacttgccgaatgattcc IGA2 28 213 13 15 5 12 8 16 5 0 13 10 JY8QFUQ01CF885 15739 productive (see comment) Homsap IGHV3-30*10 F (see comment) 706 81.02 175/216 nt 80.56 174/216 nt Homsap IGHJ1*01 F 152 76.92 40/52 nt Homsap IGHD1-1*01 F 1 8 8 16 8.8.16 [1.17.37.11] CSRGTTGTTDDSRLFQHW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (81,02% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR3-IMGT, from codon 84 of V-REGION: 1 nucleotides (from position 157 in the user submitted sequence), (cause frameshift) ctctggattcagcttcggcaattacgccatgcactgggtccgccaggctccaggcaagggcctagagtgggtggctgtcataaataaggctgggaagactaaacattatatagactccgtgaagggccgattcaacgtctccagagacaactccgaaaaacactctatttggagatgaataacgtgggagttgaggacacggctgtatattattgttcgagaggtaccacggggacgacagacgactcgaggttgttccaacactggggccagggcgccctggtcaccgtttcgtcaacatccccgaccagcccccaaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttc NA IGA2 79 84 [296, 297, 295] c86>g,T29>S(+ - +)|a103>g,S35>G(+ + -)|t105>c,S35>G(+ + -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t111>c|t114>c|g147>c|a162>t|t165>c|t169>a,S57>N(- - -)|c170>a,S57>N(- - -)|a171>t,S57>N(- - -)|t172>a,Y58>K(- - -)|t174>g,Y58>K(- - -)|a176>c,D59>A(- - -)|a186>g|g188>a,S63>K(- - -)|t189>g,S63>K(- - -)|a191>c,N64>T(- + -)|t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|c203>t,T68>I(- - -)|c230>a,T77>N(- + -)|a232>g,I78>V(+ - +)|t246>c|a250>g|c255>a,N85>K(+ - -)|g258>a|g261>c|c265>t|c268>g,Q90>E(+ + -)|a270>g,Q90>E(+ + -)|c276>t|g278>a,S93>N(- - -)|c280>g,L94>V(+ - +)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g303>a|c309>t|g313>t,A105>S(- + -)| c86>g,T29>S(+ - +)|a103>g,S35>G(+ + -)|t105>c,S35>G(+ + -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|t111>c|t114>c| g147>c|a162>t|t165>c| t169>a,S57>N(- - -)|c170>a,S57>N(- - -)|a171>t,S57>N(- - -)|t172>a,Y58>K(- - -)|t174>g,Y58>K(- - -)|a176>c,D59>A(- - -)|a186>g|g188>a,S63>K(- - -)|t189>g,S63>K(- - -)|a191>c,N64>T(- + -)| t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|c203>t,T68>I(- - -)|c230>a,T77>N(- + -)|a232>g,I78>V(+ - +)|t246>c|a250>g|c255>a,N85>K(+ - -)|g258>a|g261>c|c265>t|c268>g,Q90>E(+ + -)|a270>g,Q90>E(+ + -)|c276>t|g278>a,S93>N(- - -)|c280>g,L94>V(+ - +)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g303>a|c309>t| g313>t,A105>S(- + -)|a320>g| 319 (320) 223 (224) 181 42 (43) 15 (16) 27 6 (7) 5 8 6 2 3 2 2 2 3 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 2 5 1 1 1 3 0 0 0 0 0 1 0 0 51 51 48 3 3 0 0 0 0 1 0 0 1 0 1 0 0 0 30 24 14 10 1 9 1 1 0 0 2 1 1 2 0 0 0 2 117 113 92 21 9 12 4 3 7 2 0 2 0 0 1 2 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 0 0 0 0 0 0 0 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagcttcggcaattacgcc atgcactgggtccgccaggctccaggcaagggcctagagtgggtggctgtc ataaataaggctgggaagactaaa cattatatagactccgtgaagggccgattcaacgtctccagagacaactccgaaaaacactctatttggagatgaataacgtgggagttgaggacacggctgtatattattgt tcgagaggtaccacggggacgacagacgactcgaggttgttccaacac SRGTTGTTDDSRLFQH IGHV3-30 IGHD1-1 IGHJ1 ggattcagcttcggcaattacgcc atgcactgggtccgccaggctccaggcaagggcctagagtgggtggctgtc ataaataaggctgggaagactaaa cattatatagactccgtgaagggccgattcaacgtctccagagacaactccgaaaaacactctatttggagatgaataacgtgggagttgaggacacggctgtatattattgt tcgagaggtaccacggggacgacagacgactcgaggttgttccaacac IGA2 41 212 25 16 13 21 12 20 12 3 12 14 JY8QFUQ01CGAYM 3499 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 837 87.56 190/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD3-22*01 F 2 8 8 13 8.8.13 [1.17.38.11] CAKQKHSYGYYFDQW in-frame - ctctggattcacgtttaaaacacatgccatgagctgggtccgccaggctccaggaaaggggctggagtgggtctcaaatatcagtggcagtggtggcacaacaaattacgcggagtccgtgaagggccggttcaccatctccagagacaatgacaagaatatcctgtatctacaaatgaacaccctgagagtcgaggacacggccatatattactgtgcgaaacaaaaacatagttatggatactactttgaccagtggggccagggaacccgggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] c87>g|g104>a,S35>K(- - -)|c105>a,S35>K(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|t109>c,Y37>H(+ - -)|g141>a|g163>a,A55>N(- - -)|c164>a,A55>N(- - -)|t168>c|t174>c|t189>c|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)|t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|t247>g,S83>D(- - -)|c248>a,S83>D(- - -)|c255>t|c257>t,T86>I(- - -)|g258>c,T86>I(- - -)|g267>a|g278>c,S93>T(+ - +)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)| c87>g|g104>a,S35>K(- - -)|c105>a,S35>K(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|t109>c,Y37>H(+ - -)| g141>a|g163>a,A55>N(- - -)|c164>a,A55>N(- - -)| t168>c|t174>c|t189>c|g191>c,S64>T(+ - +)|c192>a,S64>T(+ - +)| t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|t247>g,S83>D(- - -)|c248>a,S83>D(- - -)|c255>t|c257>t,T86>I(- - -)|g258>c,T86>I(- - -)|g267>a|g278>c,S93>T(+ - +)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)| g319>c| 318 (320) 223 (225) 196 (197) 27 (28) 8 (9) 19 1 5 4 4 0 5 0 1 4 (5) 2 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 1 5 0 1 0 1 0 2 0 0 1 1 0 0 51 51 48 3 1 2 0 2 0 0 0 1 0 0 0 0 0 0 30 24 19 5 3 2 0 0 0 3 0 1 0 0 1 0 0 0 117 114 101 13 3 10 1 2 4 0 0 1 0 1 2 1 0 1 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgtttaaaacacatgcc atgagctgggtccgccaggctccaggaaaggggctggagtgggtctcaaat atcagtggcagtggtggcacaaca aattacgcggagtccgtgaagggccggttcaccatctccagagacaatgacaagaatatcctgtatctacaaatgaacaccctgagagtcgaggacacggccatatattactgt gcgaaacaaaaacatagttatggatactactttgaccag AKQKHSYGYYFDQ IGHV3-23 IGHD3-22 IGHJ4 ggattcacgtttaaaacacatgcc atgagctgggtccgccaggctccaggaaaggggctggagtgggtctcaaat atcagtggcagtggtggcacaaca aattacgcggagtccgtgaagggccggttcaccatctccagagacaatgacaagaatatcctgtatctacaaatgaacaccctgagagtcgaggacacggccatatattactgt gcgaaacaaaaacatagttatggatactactttgaccag IGA1 27 213 14 13 9 20 5 7 4 4 12 7 JY8QFUQ01CGPNS 14958 productive Homsap IGHV4-61*02 F 857 87.78 194/221 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-19*01 F 1 10 7 11 10.7.11 [1.17.38.11] CARSGWDNQFDFW in-frame - tctctggtgtcgccaccagtagtggcacttactactggagctggatccggcagtccgccggggcgggactagagtggattgggcgcatctataccggtcacaccaccatttacaacccctccctcaagggtcgagtcaccatgtcacttgacatgtccaagaaccagatctccctgaggctgacctctgtgaccgccgcagatacggccgtgtattactgtgcgcgaagtggctgggacaatcaatttgacttctggggccagggaaccctggtcaccgtctcctcagcttccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 93 100 [286] g83>t,G28>V(- - -)|t85>g,S29>A(- + -)|t89>c,I30>T(- - -)|c93>t|t105>c|g107>c,S36>T(+ - +)|c133>t,P45>S(+ - -)|a142>g,K48>A(- - -)|a143>c,K48>A(- - -)|g150>a|t165>c|a175>g,S59>G(+ + -)|g187>c,G63>H(+ - -)|g188>a,G63>H(+ - -)|g189>c,G63>H(+ - -)|g191>c,S64>T(+ - +)|a197>t,N66>I(- - -)|c198>t,N66>I(- - -)|a220>g,S74>G(+ + -)|a234>g,I78>M(+ + -)|g238>c,V80>L(+ - +)|a240>t,V80>L(+ - +)|c245>t,T82>M(- - -)|t259>a,F87>I(+ - -)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c294>t|a316>c| g83>t,G28>V(- - -)|t85>g,S29>A(- + -)|t89>c,I30>T(- - -)|c93>t|t105>c|g107>c,S36>T(+ - +)| c133>t,P45>S(+ - -)|a142>g,K48>A(- - -)|a143>c,K48>A(- - -)|g150>a|t165>c| a175>g,S59>G(+ + -)|g187>c,G63>H(+ - -)|g188>a,G63>H(+ - -)|g189>c,G63>H(+ - -)|g191>c,S64>T(+ - +)| a197>t,N66>I(- - -)|c198>t,N66>I(- - -)|a220>g,S74>G(+ + -)|a234>g,I78>M(+ + -)|g238>c,V80>L(+ - +)|a240>t,V80>L(+ - +)|c245>t,T82>M(- - -)|t259>a,F87>I(+ - -)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c294>t| a316>c|g319>a|a320>g| 318 (320) 227 (229) 199 28 (30) 6 (8) 22 5 (6) 2 (3) 5 3 2 0 2 1 6 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 2 4 0 0 1 2 0 0 0 0 1 0 1 1 51 51 46 5 2 3 1 1 1 1 1 0 0 0 0 0 0 0 30 21 16 5 0 5 1 1 0 0 0 0 0 0 3 0 0 0 117 114 103 11 1 10 3 0 3 0 0 0 2 1 2 0 0 0 6 (8) 6 5 1 (3) 1 (3) 0 0 (1) 0 (1) 0 0 1 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgtcgccaccagtagtggcacttactac tggagctggatccggcagtccgccggggcgggactagagtggattgggcgc atctataccggtcacaccacc atttacaacccctccctcaagggtcgagtcaccatgtcacttgacatgtccaagaaccagatctccctgaggctgacctctgtgaccgccgcagatacggccgtgtattactgt gcgcgaagtggctgggacaatcaatttgacttc ARSGWDNQFDF IGHV4-61 IGHD6-19 IGHJ4 ggtgtcgccaccagtagtggcacttactac tggagctggatccggcagtccgccggggcgggactagagtggattgggcgc atctataccggtcacaccacc atttacaacccctccctcaagggtcgagtcaccatgtcacttgacatgtccaagaaccagatctccctgaggctgacctctgtgaccgccgcagatacggccgtgtattactgt gcgcgaagtggctgggacaatcaatttgacttc IGG2 27 216 15 12 7 14 8 13 3 2 13 9 JY8QFUQ01CGQAI 18168 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 792 85.25 185/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-10*01 F 2 8 8 15 8.8.15 [1.17.38.11] CVRVKRFGSGNVLFDYW in-frame - ctccggtttcacgtttgacaactatgccatgacttgggtccgccagactccagggaaggggctgcagtggctctcaactattactgcttatgggactctcacatactacgctgcctccgtgaagggccggttcaccctctccagggacaactccaacaacacggtgtatctgcaaatggacagtctgagagccgaagacacggccgtattttactgtgtgagagttaaacggttcggttcggggaatgtactctttgactactggggccagggaaccctggtcaccgtctcgtcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [293] t78>c|a81>t|c87>g|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g133>a,A45>T(- - -)|g151>c,E51>Q(+ + -)|g157>c,V53>L(+ - +)|g163>a,A55>T(- - -)|g170>c,S57>T(+ - +)|g173>c,G58>A(- + -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|t186>g|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|a190>c,S64>L(- - -)|g191>t,S64>L(- - -)|a204>t|a206>c,D69>A(- - -)|a232>c,I78>L(+ + +)|a240>g|t246>c|g252>c,K84>N(+ - -)|c259>g,L87>V(+ - +)|a274>g,N92>D(+ + -)|c279>t|g291>a|a305>t,Y102>F(- + -)|c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)| t78>c| a81>t|c87>g|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g133>a,A45>T(- - -)|g151>c,E51>Q(+ + -)|g157>c,V53>L(+ - +)|g163>a,A55>T(- - -)| g170>c,S57>T(+ - +)|g173>c,G58>A(- + -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|t186>g|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|a190>c,S64>L(- - -)|g191>t,S64>L(- - -)| a204>t|a206>c,D69>A(- - -)|a232>c,I78>L(+ + +)|a240>g|t246>c|g252>c,K84>N(+ - -)|c259>g,L87>V(+ - +)|a274>g,N92>D(+ + -)|c279>t|g291>a|a305>t,Y102>F(- + -)| c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)|a320>t| 319 (320) 224 (225) 190 34 (35) 9 (10) 25 4 7 3 2 3 0 4 (5) 0 7 2 1 1 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 19 5 2 3 1 2 0 0 0 0 1 0 0 1 0 0 51 51 45 6 0 6 0 2 1 0 0 0 0 0 3 0 0 0 30 24 15 9 1 8 0 2 0 0 1 0 1 0 3 0 1 1 117 114 103 11 5 6 2 1 1 1 2 0 2 0 1 1 0 0 7 (8) 7 5 2 (3) 0 (1) 2 1 0 1 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggtttcacgtttgacaactatgcc atgacttgggtccgccagactccagggaaggggctgcagtggctctcaact attactgcttatgggactctcaca tactacgctgcctccgtgaagggccggttcaccctctccagggacaactccaacaacacggtgtatctgcaaatggacagtctgagagccgaagacacggccgtattttactgt gtgagagttaaacggttcggttcggggaatgtactctttgactac VRVKRFGSGNVLFDY IGHV3-23 IGHD3-10 IGHJ4 ggtttcacgtttgacaactatgcc atgacttgggtccgccagactccagggaaggggctgcagtggctctcaact attactgcttatgggactctcaca tactacgctgcctccgtgaagggccggttcaccctctccagggacaactccaacaacacggtgtatctgcaaatggacagtctgagagccgaagacacggccgtattttactgt gtgagagttaaacggttcggttcggggaatgtactctttgactac IGA1 31 213 13 18 9 19 4 12 5 3 12 11 JY8QFUQ01CGQES 15853 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 959 93.58 204/218 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD4-23*01 ORF 2 8 8 18 8.8.18 [1.17.38.11] CVRESGDYGGKYSKSYFDYW in-frame + cctctggattcactttcagtagttatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcaattactagaagttctagttatatgtcctacgcagactcagtgaagggccgattcaccatctccagagacaatgccgagaattcactgtatctgcaaatggacagcctgagagccgaggacacggctgtgtattactgtgtgagagaatcgggtgactacggtggtaaatatagcaagtcatactttgactattggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 94 [303, 304] c87>t|c108>t|c165>a|g170>c,S57>T(+ - +)|t174>a,S58>R(- - -)|a184>t|g185>c|c192>t|a195>g,I65>M(+ + -)|a197>c,Y66>S(+ - -)|c246>t|a250>g,K84>E(+ - -)|c255>t|a274>g,N92>D(+ + -)|c314>t,A105>V(+ - +)| c87>t|c108>t| c165>a| g170>c,S57>T(+ - +)|t174>a,S58>R(- - -)|a184>t|g185>c|c192>t|a195>g,I65>M(+ + -)| a197>c,Y66>S(+ - -)|c246>t|a250>g,K84>E(+ - -)|c255>t|a274>g,N92>D(+ + -)| c314>t,A105>V(+ - +)| 320 226 211 15 8 7 3 0 6 0 1 1 1 1 2 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 2 0 0 0 2 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 30 24 18 6 3 3 1 0 1 0 0 0 1 1 2 0 0 0 117 114 109 5 2 3 2 0 2 0 1 0 0 0 0 0 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcactttcagtagttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatca attactagaagttctagttatatg tcctacgcagactcagtgaagggccgattcaccatctccagagacaatgccgagaattcactgtatctgcaaatggacagcctgagagccgaggacacggctgtgtattactgt gtgagagaatcgggtgactacggtggtaaatatagcaagtcatactttgactat VRESGDYGGKYSKSYFDY IGHV3-21 IGHD4-23 IGHJ4 ggattcactttcagtagttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatca attactagaagttctagttatatg tcctacgcagactcagtgaagggccgattcaccatctccagagacaatgccgagaattcactgtatctgcaaatggacagcctgagagccgaggacacggctgtgtattactgt gtgagagaatcgggtgactacggtggtaaatatagcaagtcatactttgactat IGG1 14 213 8 6 5 8 3 6 3 5 3 3 JY8QFUQ01CH326 15831 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 1056 99.53 213/214 nt NA Homsap IGHJ2*01 F 256 98.11 52/53 nt Homsap IGHD2-15*01 F 2 8 8 19 8.8.19 [0.17.38.11] CARLHGYCGGGYCYDWYFDLW in-frame + tggatacagctttaccagctactggatcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatcatctatcctggtgactctgattccagatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcagcaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatgtattactgtgcgagactccatgggtattgtggtggtggttattgctacgactggtacttcgatctctggggccgtggcaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 100 100 [302] a193>t,T65>S(+ - +)| a193>t,T65>S(+ - +)| a320>t| 319 (320) 221 (222) 220 1 (2) 0 (1) 1 0 0 0 0 0 0 1 (2) 0 0 0 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 23 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacagctttaccagctactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgattcc agatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcagcaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatgtattactgt gcgagactccatgggtattgtggtggtggttattgctacgactggtacttcgatctc ARLHGYCGGGYCYDWYFDL IGHV5-51 IGHD2-15 IGHJ2 ggatacagctttaccagctactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgattcc agatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcagcaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccatgtattactgt gcgagactccatgggtattgtggtggtggttattgctacgactggtacttcgatctc IGA2 1 213 0 1 0 0 0 1 0 0 0 1 JY8QFUQ01CH338 12613 productive (see comment) Homsap IGHV3-64*02 F 967 94.44 204/216 nt 93.52 202/216 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-22*01 F 2 7 8 10 7.8.10 [1.17.38.11] CVTCGDYSYEYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 59 to 59 in the user submitted sequence), (cause frameshift); in CDR1-IMGT, from codon 29 of V-REGION: 1 nucleotides (from position 12 in the user submitted sequence), (cause frameshift) ctctggattcatttcagtacttatcctatgcactgggtccgccaggctccagggaaggGgactggaatatgtttcagctattagtcgtaatggggataacgcatattatgcagactctgtgaagggcagattcaccatgtccagagacaattccaagagcacactgtatcttcagatgggcagcctgagagctgaggacatggctgtgtattactgtgtgacatgtggtgattacagctatgagtactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [278] c87>t|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>c,A38>P(- - -)|a172>c,S58>R(- - -)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|a193>g,T65>A(- - -)|c234>g,I78>M(+ + -)|a254>g,N85>S(- - -)|g258>a|a270>g|c314>t,A105>V(+ - +)| c87>t|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>c,A38>P(- - -)| a172>c,S58>R(- - -)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|a193>g,T65>A(- - -)| c234>g,I78>M(+ + -)|a254>g,N85>S(- - -)|g258>a|a270>g| c314>t,A105>V(+ - +)|g317>c,R106>T(- - -)|g319>t|a320>g| 316 (320) 220 (224) 207 (208) 13 (16) 3 (5) 10 (11) 3 (4) 3 3 0 1 0 0 0 2 (3) 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 19 4 1 3 0 0 2 0 0 0 0 0 2 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 2 0 0 1 0 0 0 0 0 0 0 117 114 110 4 2 2 2 1 0 0 0 0 0 0 0 1 0 0 4 (8) 4 3 (4) 1 (4) 0 (2) 1 (2) 0 (1) 0 1 0 0 0 0 0 0 (1) 0 0 (1) 0 aa,26-27(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|aa,152-153(FR2)|ta,154-155(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,154-155(FR2)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|tt,198-199(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|agct,162-165(FR2)|agta,169-172(CDR2)|ggta,187-190(CDR2)|ggca,220-223(FR3)|ggca,274-277(FR3)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|agct,162-165(FR2)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatttcagtacttatcct atgcactgggtccgccaggctccagggaagggactggaatatgtttcagct attagtcgtaatggggataacgca tattatgcagactctgtgaagggcagattcaccatgtccagagacaattccaagagcacactgtatcttcagatgggcagcctgagagctgaggacatggctgtgtattactgt gtgacatgtggtgattacagctatgagtac VTCGDYSYEY IGHV3-64 IGHD3-22 IGHJ4 ggattcatttcagtacttatcct atgcactgggtccgccaggctccagggaagggactggaatatgtttcagct attagtcgtaatggggataacgca tattatgcagactctgtgaagggcagattcaccatgtccagagacaattccaagagcacactgtatcttcagatgggcagcctgagagctgaggacatggctgtgtattactgt gtgacatgtggtgattacagctatgagtac IGA1 12 212 8 4 5 8 3 4 2 1 2 7 JY8QFUQ01CHBJS 17089 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23*05 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 896 90.37 197/218 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD3-3*01 F 2 8 8 13 8.8.13 [1.17.38.11] CAKRRGGYYIDFDYW in-frame + cctctggattcacctttactagttacagtttcaattgggtccgccaggctccagggaaggggctggagtgggtctcaactatcaatactagtggtagtagcacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacgaggtgtatctgcaaatgaacagcctgagagccgaggacacggccatatattactgtgcgaagcggaggggtggttattacatcgactttgactactggggccagggaacgctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [288] g104>c,S35>T(+ - +)|c105>t,S35>T(+ - +)|c108>t|t111>c|g112>a,A38>S(- + -)|c113>g,A38>S(- + -)|c114>t,A38>S(- + -)|a115>t,M39>F(+ - -)|g117>c,M39>F(+ - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g163>a,A55>T(- - -)|t168>c|g170>a,S57>N(- - -)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|g187>a,G63>S(+ + -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|c259>g,L87>V(+ - +)|g301>a,V101>I(+ - +)| g104>c,S35>T(+ - +)|c105>t,S35>T(+ - +)|c108>t|t111>c|g112>a,A38>S(- + -)|c113>g,A38>S(- + -)|c114>t,A38>S(- + -)| a115>t,M39>F(+ - -)|g117>c,M39>F(+ - -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g163>a,A55>T(- - -)| t168>c|g170>a,S57>N(- - -)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|g187>a,G63>S(+ + -)| a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|c259>g,L87>V(+ - +)|g301>a,V101>I(+ - +)| a318>g|g319>c|a320>g| 317 (320) 223 (226) 202 21 (24) 3 (6) 18 1 (3) 7 4 2 0 1 1 0 3 (4) 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 2 5 0 1 3 1 0 0 0 0 1 1 0 0 51 51 46 5 0 5 0 2 1 0 0 0 1 0 1 0 0 0 30 24 19 5 1 4 0 3 0 1 0 0 0 0 1 0 0 0 117 114 110 4 0 4 1 1 0 0 0 1 0 0 0 1 0 0 5 (8) 5 5 0 (3) 0 (3) 0 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttactagttacagt ttcaattgggtccgccaggctccagggaaggggctggagtgggtctcaact atcaatactagtggtagtagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacgaggtgtatctgcaaatgaacagcctgagagccgaggacacggccatatattactgt gcgaagcggaggggtggttattacatcgactttgactac AKRRGGYYIDFDY IGHV3-23 IGHD3-3 IGHJ4 ggattcacctttactagttacagt ttcaattgggtccgccaggctccagggaaggggctggagtgggtctcaact atcaatactagtggtagtagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacgaggtgtatctgcaaatgaacagcctgagagccgaggacacggccatatattactgt gcgaagcggaggggtggttattacatcgactttgactac IGA1 21 213 14 7 11 17 3 4 0 3 9 9 JY8QFUQ01CHFYS 9182 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 707 80.73 176/218 nt 80.28 175/218 nt Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD2-21*02 F 2 8 8 15 8.8.15 [1.17.38.11] CARYRVGDSLYYGLDGW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (80,73% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR2-IMGT at codon 59 of V-REGION: 'ACT' (3 nucleotides at position 90 to 92 in the user submitted sequence), (do not cause frameshift); cctctggattcattttagaaaattttgccatgagttggctccgccaggcaccagggaaggggctggaatgggtctcgactatcagcagcACTagtggtgacacggcatattactcagactccgtgaggggccgcttttccatctccagagacaactccaagagcactctgttcttgcagatgaacagcctgagtgccgaagacacggccatttactactgtgcgagataccgtgtgggtgacagtctctactacggtttggacggctggggccaagggaccacggtcaccgtctcatcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [297] c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|t90>a,F30>L(+ - -)|a103>g,S35>E(- - -)|g104>a,S35>E(- - -)|c105>a,S35>E(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a110>t,Y37>F(- + -)|c120>t|g124>c,V42>L(+ - +)|t135>a|g153>a|a162>g|g163>a,A55>T(- - -)|t168>c|t171>c|g172>a,G58>S(+ + -)|t174>c,G58>S(+ + -)|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|g191>c,S64>T(+ - +)|c192>g,S64>T(+ - +)|a193>g,T65>A(- - -)|c198>t|g202>t,A68>S(- + -)|a215>g,K72>R(+ + +)|g225>c|c228>t|a229>t,T77>S(+ - +)|t246>c|a254>g,N85>S(- - -)|g258>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|a270>g|a285>t,R95>S(- - -)|g291>a|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)|t306>c| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|t90>a,F30>L(+ - -)|a103>g,S35>E(- - -)|g104>a,S35>E(- - -)|c105>a,S35>E(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a110>t,Y37>F(- + -)| c120>t|g124>c,V42>L(+ - +)|t135>a|g153>a|a162>g|g163>a,A55>T(- - -)| t168>c|t171>c|g172>a,G58>S(+ + -)|t174>c,G58>S(+ + -)|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|g191>c,S64>T(+ - +)|c192>g,S64>T(+ - +)|a193>g,T65>A(- - -)| c198>t|g202>t,A68>S(- + -)|a215>g,K72>R(+ + +)|g225>c|c228>t|a229>t,T77>S(+ - +)|t246>c|a254>g,N85>S(- - -)|g258>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|a270>g|a285>t,R95>S(- - -)|g291>a|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)|t306>c| a317>g,K106>R(+ + +)|g319>t| 316 (320) 222 (226) 180 (182) 42 (44) 15 (16) 27 (28) 6 (7) 8 7 7 0 1 5 2 3 1 2 (3) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 15 9 0 9 1 2 3 0 0 1 1 1 0 0 0 0 51 51 45 6 4 2 1 2 1 0 0 0 0 1 1 0 0 0 30 24 15 9 2 7 1 2 0 4 0 0 0 0 1 1 0 0 117 114 96 18 9 9 3 2 3 3 0 0 4 0 1 0 2 0 4 (8) 4 4 (6) 0 (2) 0 (1) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcattttagaaaattttgcc atgagttggctccgccaggcaccagggaaggggctggaatgggtctcgact atcagcagcagtggtgacacggca tattactcagactccgtgaggggccgcttttccatctccagagacaactccaagagcactctgttcttgcagatgaacagcctgagtgccgaagacacggccatttactactgt gcgagataccgtgtgggtgacagtctctactacggtttggacggc ARYRVGDSLYYGLDG IGHV3-23 IGHD2-21 IGHJ6 ggattcattttagaaaattttgcc atgagttggctccgccaggcaccagggaaggggctggaatgggtctcgact atcagcagcagtggtgacacggca tattactcagactccgtgaggggccgcttttccatctccagagacaactccaagagcactctgttcttgcagatgaacagcctgagtgccgaagacacggccatttactactgt gcgagataccgtgtgggtgacagtctctactacggtttggacggc IGA2 42 213 28 14 15 22 13 20 13 2 11 16 JY8QFUQ01CHKDD 13329 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 964 93.61 205/219 nt 93.15 204/219 nt Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD3-10*01 F 2 8 8 17 8.8.17 [2.17.38.11] CAKKYHYGSGTYYYHFDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'G' (1 nucleotides at position 16 to 16 in the user submitted sequence), (cause frameshift); gcctctggattcaccGtttagcaactatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtcgcaactattatttatagtggcgatagtacatactacgcagattccgtgaggggccggttcaccatctccagagacaattccaagaacactctgcatctgcatatgaacagcctgagagccgaggacacggccgtatattactgtgcgaaaaagtatcactatggttcggggacttattattaccacttcgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [302] g107>a,S36>N(- - -)|t160>g,S54>A(- + -)|g163>a,A55>T(- - -)|g170>t,S57>I(- - -)|g172>t,G58>Y(+ - -)|g173>a,G58>Y(+ - -)|t186>c|g188>a,G63>D(- - -)|c192>t|c207>t|a215>g,K72>R(+ + +)|g258>t|t262>c,Y88>H(+ - -)|a270>t,Q90>H(- + -)| g107>a,S36>N(- - -)| t160>g,S54>A(- + -)|g163>a,A55>T(- - -)| g170>t,S57>I(- - -)|g172>t,G58>Y(+ - -)|g173>a,G58>Y(+ - -)|t186>c|g188>a,G63>D(- - -)|c192>t| c207>t|a215>g,K72>R(+ + +)|g258>t|t262>c,Y88>H(+ - -)|a270>t,Q90>H(- + -)| g319>a| 318 (320) 225 (227) 211 (212) 14 (15) 4 (5) 10 1 4 (5) 2 2 0 0 1 0 0 0 3 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 30 24 18 6 2 4 0 2 1 1 0 0 0 0 0 0 2 0 117 114 109 5 2 3 1 0 1 1 0 0 1 0 0 0 1 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtcgcaact attatttatagtggcgatagtaca tactacgcagattccgtgaggggccggttcaccatctccagagacaattccaagaacactctgcatctgcatatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaaaagtatcactatggttcggggacttattattaccacttcgactac AKKYHYGSGTYYYHFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcacctttagcaactatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtcgcaact attatttatagtggcgatagtaca tactacgcagattccgtgaggggccggttcaccatctccagagacaattccaagaacactctgcatctgcatatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaaaagtatcactatggttcggggacttattattaccacttcgactac IGA2 14 213 9 5 6 9 3 5 2 2 5 5 JY8QFUQ01CHVJ1 11338 productive (see comment) Homsap IGHV3-7*02 F 917 92.09 198/215 nt 91.63 197/215 nt Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD5-12*01 F 1 7 8 9 7.8.9 [1.17.38.11] CARDQVTEDYW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 29 of V-REGION: 1 nucleotides (from position 10 in the user submitted sequence), (cause frameshift) tctggattccttttagaacctattggatgagttgggtccgccaggctccagggaaagggctggagtgggtggcccacataaaccaagatggacgtgaggcatactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgtgccagagatcaagtgactgaagactactggggccagggaaccctggtcagtgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG2 100 100 [274] c87>t|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|c120>t|a163>c,N55>H(- - -)|g171>c,K57>N(+ - -)|a187>c,S63>R(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)|c248>g,A83>G(- + -)|c255>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|g297>c|g301>a,V101>M(+ - -)|c309>t|g315>c| c87>t|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)| c120>t|a163>c,N55>H(- - -)| g171>c,K57>N(+ - -)|a187>c,S63>R(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)| c248>g,A83>G(- + -)|c255>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|g297>c|g301>a,V101>M(+ - -)|c309>t| g315>c| 318 221 203 18 7 11 1 2 5 0 3 0 0 1 4 2 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 20 3 1 2 0 0 1 0 0 0 0 1 1 0 0 0 51 51 49 2 1 1 0 0 1 0 1 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 0 0 2 0 0 0 1 0 0 0 117 114 106 8 4 4 0 2 3 0 0 0 0 0 1 2 0 0 6 6 5 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattccttttagaacctattgg atgagttgggtccgccaggctccagggaaagggctggagtgggtggcccac ataaaccaagatggacgtgaggca tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac ARDQVTEDY IGHV3-7 IGHD5-12 IGHJ4 ggattccttttagaacctattgg atgagttgggtccgccaggctccagggaaagggctggagtgggtggcccac ataaaccaagatggacgtgaggca tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac IGG2 17 212 8 9 7 12 1 5 5 1 5 6 JY8QFUQ01CI12B 18802 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 1008 98.56 205/208 nt NA Homsap IGHJ6*02 F 229 85.48 53/62 nt Homsap IGHD4-17*01 F 2 6 8 16 6.8.16 [X.17.38.11] CARQLDYGDYSRHGLDVW in-frame + caactttaccggctactggatcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatcatctatcctggtgactctgataccagatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcagcaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccgtgtattactgtgcgagacaattagactacggtgactactctaggcacggtttggacgtctggggccaagggaccacggtcaccgtctcctcagcatcccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatggggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 100 89 [288, 289] g86>a,S29>N(- - -)|a106>g,S36>G(+ + -)|a301>g,M101>V(+ - -)| g86>a,S29>N(- - -)|a106>g,S36>G(+ + -)| a301>g,M101>V(+ - -)| 320 216 213 3 0 3 2 1 0 0 0 0 0 0 0 0 0 0 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 19 17 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 113 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| caactttaccggctactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgatacc agatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcagcaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccgtgtattactgt gcgagacaattagactacggtgactactctaggcacggtttggacgtc ARQLDYGDYSRHGLDV IGHV5-51 IGHD4-17 IGHJ6 caactttaccggctactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgatacc agatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcagcaccgcctacctgcagtggagcagcctgaaggcctcggacaccgccgtgtattactgt gcgagacaattagactacggtgactactctaggcacggtttggacgtc IGA1 3 208 3 0 1 1 2 2 0 0 1 2 JY8QFUQ01CIMAM 19491 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 899 91.16 196/215 nt NA Homsap IGHJ3*02 F 214 92 46/50 nt Homsap IGHD2-21*01 F 2 8 7 15 8.7.15 [1.17.38.11] CTRELRLAAARDGVDIW in-frame - tttctgggttcaccgtcagtagcaagttcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaattacttatcctgatggtactacatattatggagactccgtgaagggccgattcaccatctccagagacaattccaagaacacactggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgtacgagagaacttaggctggctgctgcgcgtgatggtgttgacatctggggccaggggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [291] c74>t|c75>t|c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)|g163>a,V55>I(+ - +)|t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|c198>t|c201>t|c203>g,A68>G(- + -)|g258>a|t262>g,Y88>D(- - -)|g303>t|c309>t|g313>a,A105>T(- - -)| c74>t|c75>t| c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)| g163>a,V55>I(+ - +)| t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)| c198>t|c201>t|c203>g,A68>G(- + -)|g258>a|t262>g,Y88>D(- - -)|g303>t|c309>t| g313>a,A105>T(- - -)| 320 223 203 20 7 13 0 4 7 1 1 0 1 0 2 2 1 1 78 5 3 2 2 0 0 0 2 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 21 14 7 0 7 0 1 2 1 1 0 0 0 2 0 0 0 117 114 107 7 5 2 0 1 3 0 0 0 0 0 0 1 1 1 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tttct gggttcaccgtcagtagcaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaatt acttatcctgatggtactaca tattatggagactccgtgaagggccgattcaccatctccagagacaattccaagaacacactggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttaggctggctgctgcgcgtgatggtgttgacatc TRELRLAAARDGVDI IGHV3-53 IGHD2-21 IGHJ3 gggttcaccgtcagtagcaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaatt acttatcctgatggtactaca tattatggagactccgtgaagggccgattcaccatctccagagacaattccaagaacacactggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttaggctggctgctgcgcgtgatggtgttgacatc IGA1 17 210 9 8 8 13 1 4 5 0 3 9 JY8QFUQ01CIOY1 10543 productive (see comment) Homsap IGHV3-48*02 F 973 94.06 206/219 nt 93.61 205/219 nt Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD3-3*01 F 3 8 8 20 8.8.20 [2.17.38.11] CARARRTISGVVFIEHYGMDVW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 66 of V-REGION: 'A' (1 nucleotides at position 106 to 106 in the user submitted sequence), (cause frameshift); gcctctggattcaccttcaggacccatagcatgaactgggtccgccaggctccagggaaggggctggagtggatttcattcattagtagtagtagtggtaccataAttttatgcagactctgtgaagggccggttcaccatctccagagacaatgccaagaactcactttatctgcaaatgaacagcctgagagacgaggatgcggctgtgtattactgtgcgagagcccgtaggacgatttctggagtggtttttattgagcactacggtatggacgtctggggcccagggaccacggtctccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [311] t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|t109>c,Y37>H(+ - -)|g157>a,V53>I(+ - +)|a164>t,Y55>F(- + -)|a187>g,S63>G(+ + -)|a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|c201>t|a225>g|g261>t|c294>t|a295>g,T99>A(- - -)| t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|t109>c,Y37>H(+ - -)| g157>a,V53>I(+ - +)|a164>t,Y55>F(- + -)| a187>g,S63>G(+ + -)| a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|c201>t|a225>g|g261>t|c294>t|a295>g,T99>A(- - -)| a320>c| 319 (320) 226 (227) 213 13 (14) 4 (5) 9 3 1 3 1 0 (1) 0 2 0 1 0 1 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 1 0 0 0 0 1 0 0 1 51 51 49 2 0 2 0 1 0 0 0 0 1 0 0 0 0 0 30 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 117 114 107 7 4 3 2 0 3 0 0 0 1 0 0 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| gcctct ggattcaccttcaggacccatagc atgaactgggtccgccaggctccagggaaggggctggagtggatttcattc attagtagtagtagtggtaccata ttttatgcagactctgtgaagggccggttcaccatctccagagacaatgccaagaactcactttatctgcaaatgaacagcctgagagacgaggatgcggctgtgtattactgt gcgagagcccgtaggacgatttctggagtggtttttattgagcactacggtatggacgtc ARARRTISGVVFIEHYGMDV IGHV3-48 IGHD3-3 IGHJ6 ggattcaccttcaggacccatagc atgaactgggtccgccaggctccagggaaggggctggagtggatttcattc attagtagtagtagtggtaccata ttttatgcagactctgtgaagggccggttcaccatctccagagacaatgccaagaactcactttatctgcaaatgaacagcctgagagacgaggatgcggctgtgtattactgt gcgagagcccgtaggacgatttctggagtggtttttattgagcactacggtatggacgtc IGA1 13 213 8 5 4 6 4 7 4 0 5 4 JY8QFUQ01CIQUI 19472 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 999 95.85 208/217 nt NA Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [1.17.38.11] CAKDGARGGGSCFNSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcacctttagaagctatgccatgagctgggtccgccaggttccagggaaggggctggagtgggtctcagctattagtggtaatggtgctaacacatactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgtgcgaaagatggcgctagaggtggcggcagttgctttaactcctggggccagggaaccctggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG4 100 88 [291] c105>a,S35>R(- - -)|c134>t,A45>V(+ - +)|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|g191>a,S64>N(- - -)|g225>a|c248>t,S83>F(- - -)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)| c105>a,S35>R(- - -)| c134>t,A45>V(+ - +)| g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|g191>a,S64>N(- - -)| g225>a|c248>t,S83>F(- - -)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)| 320 225 216 9 1 8 0 3 2 0 1 1 0 0 2 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 2 0 0 0 0 0 0 1 0 0 0 117 114 110 4 1 3 0 1 1 0 1 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagaagctatgcc atgagctgggtccgccaggttccagggaaggggctggagtgggtctcagct attagtggtaatggtgctaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtggcggcagttgctttaactcc AKDGARGGGSCFNS IGHV3-23 IGHD5-24 IGHJ4 ggattcacctttagaagctatgcc atgagctgggtccgccaggttccagggaaggggctggagtgggtctcagct attagtggtaatggtgctaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtggcggcagttgctttaactcc IGG4 9 213 5 4 5 8 0 1 1 0 4 4 JY8QFUQ01CJ5TF 16182 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 2 8 8 15 8.8.15 [1.17.38.11] CARDMGSGRYFEPYDYW in-frame - gtctggattcatcttcttgaaatatggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaggtatatggtttgatggaagtaatacatactatgcggactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatttgcaactgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagatatgggttcgggaagatattttgaaccttatgactactggggccagggaaccccggtcaccgtctcctcagcttccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG3 100 100 [294] c86>t,T29>I(- - -)|a103>t,S35>L(- - -)|g104>t,S35>L(- - -)|t105>g,S35>L(- - -)|g107>a,S36>K(- - -)|c108>a,S36>K(- - -)|t164>g,V55>G(- - -)|a173>t,Y58>F(- + -)|a194>c,K65>T(- - -)|a204>g|c259>g,L87>V(+ - +)|c265>t|a271>c,M91>L(+ + -)| c86>t,T29>I(- - -)|a103>t,S35>L(- - -)|g104>t,S35>L(- - -)|t105>g,S35>L(- - -)|g107>a,S36>K(- - -)|c108>a,S36>K(- - -)| t164>g,V55>G(- - -)| a173>t,Y58>F(- + -)|a194>c,K65>T(- - -)| a204>g|c259>g,L87>V(+ - +)|c265>t|a271>c,M91>L(+ + -)| 320 225 212 13 2 11 1 1 2 0 2 1 2 0 0 1 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 1 1 0 0 1 1 0 0 0 1 1 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 30 24 22 2 0 2 0 0 0 0 1 0 1 0 0 0 0 0 117 114 110 4 2 2 1 0 1 0 1 0 0 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcatcttcttgaaatatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaggt atatggtttgatggaagtaataca tactatgcggactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatttgcaactgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagatatgggttcgggaagatattttgaaccttatgactac ARDMGSGRYFEPYDY IGHV3-33 IGHD3-10 IGHJ4 ggattcatcttcttgaaatatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaggt atatggtttgatggaagtaataca tactatgcggactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatttgcaactgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagatatgggttcgggaagatattttgaaccttatgactac IGG3 13 213 4 9 3 6 1 7 2 0 3 8 JY8QFUQ01CJ990 10437 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 1003 96.3 208/216 nt 95.83 207/216 nt Homsap IGHJ3*01 F 241 98 49/50 nt Homsap IGHD2-15*01 F 3 8 8 17 8.8.17 [1.16.38.11] CARDTLEVVSNMDDAFDVW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 58 in the user submitted sequence), (cause frameshift) ctctggattcaccttcagtcgttatagcatgaactgggtccgccaggctccagggaagggctggagtgggtctcatacattagtaggagtagtactgacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacggcaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtatttctgtgcgagagatactctagaggtagtgtctaatatggatgatgcttttgatgtctggggccaagggacaatggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [298] a106>c,S36>R(- - -)|c108>t,S36>R(- - -)|c164>a,S55>Y(+ - -)|t174>g,S58>R(- - -)|g188>c,S63>T(+ - +)|t190>g,Y64>D(- - -)|c248>g,A83>G(- + -)|a308>t,Y103>F(- + -)| a106>c,S36>R(- - -)|c108>t,S36>R(- - -)| c164>a,S55>Y(+ - -)| t174>g,S58>R(- - -)|g188>c,S63>T(+ - +)|t190>g,Y64>D(- - -)| c248>g,A83>G(- + -)|a308>t,Y103>F(- + -)| 320 224 216 8 0 8 0 0 1 0 1 1 1 0 1 1 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 1 0 1 0 0 0 0 0 0 0 51 50 49 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 30 24 21 3 0 3 0 0 0 0 0 0 0 0 1 0 0 2 117 114 112 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtcgttatagc atgaactgggtccgccaggctccagggaagggctggagtgggtctcatac attagtaggagtagtactgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacggcaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtatttctgt gcgagagatactctagaggtagtgtctaatatggatgatgcttttgatgtc ARDTLEVVSNMDDAFDV IGHV3-21 IGHD2-15 IGHJ3 ggattcaccttcagtcgttatagc atgaactgggtccgccaggctccagggaagggctggagtgggtctcatac attagtaggagtagtactgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacggcaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtatttctgt gcgagagatactctagaggtagtgtctaatatggatgatgcttttgatgtc IGA1 8 212 1 7 1 4 0 4 0 0 3 5 JY8QFUQ01CJG1W 11588 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 801 85.71 186/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-3*01 F 2 8 8 18 8.8.18 [1.17.38.11] CARDGVRGLGAYYIYYFDSW in-frame + ctctggattcacctttggcctctatgccatgaactgggtccgccagactccagggaaggggccggagtggctcgcaactatcagtggtagtggaagtaggtcattctacgcagactccctcaggggccgtttcaccatctccagagacaattccaagggcacggtgtacctggacatgaccaccctgggagccgacgactcggccgtgtattactgtgccagagatggagtccgtggccttggtgcttattatatctactattttgactcatggggccagggaaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtcc NA IGA1 100 100 [302] a103>g,S35>G(+ + -)|a106>c,S36>L(- - -)|g107>t,S36>L(- - -)|g119>a,S40>N(- - -)|g133>a,A45>T(- - -)|t149>c,L50>P(- - -)|g157>c,V53>L(+ - +)|t160>g,S54>A(- + -)|g163>a,A55>T(- - -)|t168>c|t186>a|g187>a,G63>S(+ + -)|c192>g,S64>R(- - -)|a193>t,T65>S(+ - +)|a197>t,Y66>F(- + -)|g211>c,V71>L(+ - +)|g213>c,V71>L(+ - +)|a215>g,K72>R(+ + +)|g225>t|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c259>g,L87>V(+ - +)|t264>c|c268>g,Q90>D(+ - -)|a270>c,Q90>D(+ - -)|a275>c,N92>T(- + -)|g278>c,S93>T(+ - +)|a283>g,R95>G(- - -)|g291>c,E97>D(+ - +)|a295>t,T99>S(+ - +)|a303>g|g315>c|a317>g,K106>R(+ + +)| a103>g,S35>G(+ + -)|a106>c,S36>L(- - -)|g107>t,S36>L(- - -)| g119>a,S40>N(- - -)|g133>a,A45>T(- - -)|t149>c,L50>P(- - -)|g157>c,V53>L(+ - +)|t160>g,S54>A(- + -)|g163>a,A55>T(- - -)| t168>c|t186>a|g187>a,G63>S(+ + -)|c192>g,S64>R(- - -)|a193>t,T65>S(+ - +)| a197>t,Y66>F(- + -)|g211>c,V71>L(+ - +)|g213>c,V71>L(+ - +)|a215>g,K72>R(+ + +)|g225>t|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c259>g,L87>V(+ - +)|t264>c|c268>g,Q90>D(+ - -)|a270>c,Q90>D(+ - -)|a275>c,N92>T(- + -)|g278>c,S93>T(+ - +)|a283>g,R95>G(- - -)|g291>c,E97>D(+ - +)|a295>t,T99>S(+ - +)|a303>g| g315>c|a317>g,K106>R(+ + +)| 320 225 192 33 6 27 7 4 0 3 3 0 3 1 6 3 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 0 0 0 1 0 0 0 0 0 1 0 51 51 45 6 0 6 0 3 0 1 0 0 0 0 1 0 0 1 30 24 19 5 2 3 0 1 0 1 0 0 1 1 0 1 0 0 117 114 97 17 3 14 5 0 0 1 2 0 2 0 4 2 1 0 8 8 6 2 1 1 1 0 0 0 0 0 0 0 1 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttggcctctatgcc atgaactgggtccgccagactccagggaaggggccggagtggctcgcaact atcagtggtagtggaagtaggtca ttctacgcagactccctcaggggccgtttcaccatctccagagacaattccaagggcacggtgtacctggacatgaccaccctgggagccgacgactcggccgtgtattactgt gccagagatggagtccgtggccttggtgcttattatatctactattttgactca ARDGVRGLGAYYIYYFDS IGHV3-23 IGHD3-3 IGHJ4 ggattcacctttggcctctatgcc atgaactgggtccgccagactccagggaaggggccggagtggctcgcaact atcagtggtagtggaagtaggtca ttctacgcagactccctcaggggccgtttcaccatctccagagacaattccaagggcacggtgtacctggacatgaccaccctgggagccgacgactcggccgtgtattactgt gccagagatggagtccgtggccttggtgcttattatatctactattttgactca IGA1 31 213 13 18 4 14 9 17 3 2 20 6 JY8QFUQ01CJV6U 19302 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 809 86.51 186/215 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-10*02 F 3 8 8 12 8.8.12 [0.17.38.11] CVKDISRSGCFEYW in-frame - ctggattccagtttagcaactatgccatgagctgggtccgtcaggctcctgggaaggggctggagtgggtctcaactattagtaaagacggtgtttacacctactaccccgactccgcgaagggccgggtcaccatctccagagacaattccaagaatacaatttatttgcaaatgaacagcctgacagccgaggacacggccagatattactgtgtgaaagacatttccaggtcaggctgctttgagtattggggtcagggaaccctgatcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [282] a85>c,T29>Q(- - -)|c86>a,T29>Q(- - -)|c87>g,T29>Q(- - -)|g107>a,S36>N(- - -)|c129>t|a138>t|g163>a,A55>T(- - -)|g172>a,G58>K(- - -)|g173>a,G58>K(- - -)|t174>a,G58>K(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|t177>c,S59>D(- - -)|g188>t,G63>V(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a195>c|g202>c,A68>P(- - -)|a204>c,A68>P(- - -)|t212>c,V71>A(+ - +)|t226>g,F76>V(+ - -)|c255>t|g258>a|c259>a,L87>I(+ + +)|g261>t,L87>I(+ + +)|c265>t|g284>c,R95>T(- - -)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)|c314>t,A105>V(+ - +)| a85>c,T29>Q(- - -)|c86>a,T29>Q(- - -)|c87>g,T29>Q(- - -)|g107>a,S36>N(- - -)| c129>t|a138>t|g163>a,A55>T(- - -)| g172>a,G58>K(- - -)|g173>a,G58>K(- - -)|t174>a,G58>K(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|t177>c,S59>D(- - -)|g188>t,G63>V(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a195>c| g202>c,A68>P(- - -)|a204>c,A68>P(- - -)|t212>c,V71>A(+ - +)|t226>g,F76>V(+ - -)|c255>t|g258>a|c259>a,L87>I(+ + +)|g261>t,L87>I(+ + +)|c265>t|g284>c,R95>T(- - -)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)| c314>t,A105>V(+ - +)| 320 223 193 30 6 24 1 8 4 2 3 2 2 1 2 1 2 2 78 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 0 0 1 1 0 0 0 1 0 0 51 51 48 3 2 1 0 1 1 0 0 0 1 0 0 0 0 0 30 24 14 10 1 9 1 4 0 1 1 0 1 1 0 0 1 0 117 114 102 12 3 9 0 2 2 1 1 1 0 0 2 0 1 2 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ct ggattccagtttagcaactatgcc atgagctgggtccgtcaggctcctgggaaggggctggagtgggtctcaact attagtaaagacggtgtttacacc tactaccccgactccgcgaagggccgggtcaccatctccagagacaattccaagaatacaatttatttgcaaatgaacagcctgacagccgaggacacggccagatattactgt gtgaaagacatttccaggtcaggctgctttgagtat VKDISRSGCFEY IGHV3-23 IGHD3-10 IGHJ4 ggattccagtttagcaactatgcc atgagctgggtccgtcaggctcctgggaaggggctggagtgggtctcaact attagtaaagacggtgtttacacc tactaccccgactccgcgaagggccgggtcaccatctccagagacaattccaagaatacaatttatttgcaaatgaacagcctgacagccgaggacacggccagatattactgt gtgaaagacatttccaggtcaggctgctttgagtat IGA2 29 213 14 15 11 18 3 11 5 1 10 13 JY8QFUQ01CJYGN 12749 productive (see comment) Homsap IGHV3-7*01 F 945 93.09 202/217 nt 92.17 200/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARETLPLGVGGFDNW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'GA' (2 nucleotides at position 20 to 21 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 46 of V-REGION: 'G' (1 nucleotides at position 54 to 54 in the user submitted sequence), (cause frameshift); ctctggattcacatttagtGAattcattggatgatctgggtccgccaggctccGagggaaggggctggagtgggtggccaacataaaccaagatggaggtgacatggcctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactctctgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgtgcgagagagacactcccccttggtgttgggggttttgacaactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [294] c87>a|g107>t,S36>I(- - -)|c108>t,S36>I(- - -)|t109>c,Y37>H(+ - -)|g119>t,S40>I(- - -)|g171>c,K57>N(+ - -)|a187>g,S63>G(+ + -)|g192>c,E64>D(+ - +)|a194>t,K65>M(- + -)|a195>g,K65>M(- + -)|t196>g,Y66>A(- - -)|a197>c,Y66>A(- - -)|a258>t|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| c87>a|g107>t,S36>I(- - -)|c108>t,S36>I(- - -)|t109>c,Y37>H(+ - -)| g119>t,S40>I(- - -)| g171>c,K57>N(+ - -)|a187>g,S63>G(+ + -)|g192>c,E64>D(+ - +)|a194>t,K65>M(- + -)|a195>g,K65>M(- + -)| t196>g,Y66>A(- - -)|a197>c,Y66>A(- - -)|a258>t|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 225 210 15 2 13 2 2 1 1 1 1 2 0 2 0 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 1 1 0 1 0 0 0 0 1 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 30 24 19 5 0 5 2 0 0 0 0 0 1 0 2 0 0 0 117 114 109 5 1 4 0 2 0 0 1 0 1 0 0 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacatttagtattcattgg atgatctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaccaagatggaggtgacatg gcctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactctctgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgt gcgagagagacactcccccttggtgttgggggttttgacaac ARETLPLGVGGFDN IGHV3-7 IGHD3-10 IGHJ4 ggattcacatttagtattcattgg atgatctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaccaagatggaggtgacatg gcctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactctctgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgt gcgagagagacactcccccttggtgttgggggttttgacaac IGG2 15 213 6 9 3 8 3 7 1 1 5 8 JY8QFUQ01CK280 12834 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 924 91.36 201/220 nt 90.91 200/220 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 2 10 7 14 10.7.14 [1.17.38.11] CARRALDSSGHYIDIW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 54 to 54 in the user submitted sequence), (cause frameshift); ctctggtggctccatgagcagtggtaattactgctggggctggggccgccagcCccccaggaaaggggctggagtggattggaagtatgtgttatggtgggagcacctactacagcttgtcccccaagggtcgagtcaccatatccatagactcgtcgaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggctgtgtattactgtgcgagacgcgcattagacagtagtggtcattacattgacatctggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtgtcatcgctgcctggtccagggcttcttccc NA IGA1 71 79 [294] c90>g,I30>M(+ + -)|a103>g,S35>G(+ + -)|g107>a,S36>N(- - -)|a113>g,Y38>C(- - -)|a124>g,I42>G(- - -)|t125>g,I42>G(- - -)|g141>a|g162>a|c168>g,I56>M(+ + -)|a170>g,Y57>C(- - -)|a175>g,S59>G(+ + -)|a203>g,N68>S(- - -)|c205>t,P69>L(- - -)|c206>t,P69>L(- - -)|t212>c,L71>P(- - -)|a220>g,S74>G(+ + -)|g238>a,V80>I(+ - +)|a244>t,T82>S(+ - +)|c249>g| c90>g,I30>M(+ + -)|a103>g,S35>G(+ + -)|g107>a,S36>N(- - -)|a113>g,Y38>C(- - -)| a124>g,I42>G(- - -)|t125>g,I42>G(- - -)|g141>a|g162>a| c168>g,I56>M(+ + -)|a170>g,Y57>C(- - -)|a175>g,S59>G(+ + -)| a203>g,N68>S(- - -)|c205>t,P69>L(- - -)|c206>t,P69>L(- - -)|t212>c,L71>P(- - -)|a220>g,S74>G(+ + -)|g238>a,V80>I(+ - +)|a244>t,T82>S(+ - +)|c249>g| a320>g| 319 (320) 227 (228) 208 19 (20) 3 (4) 16 7 (8) 4 2 1 0 0 1 0 0 3 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 0 4 2 1 0 0 0 0 0 0 0 1 0 0 51 51 47 4 2 2 1 2 0 0 0 0 0 0 0 0 0 1 30 21 18 3 0 3 2 0 0 0 0 0 0 0 0 1 0 0 117 114 106 8 1 7 2 1 2 1 0 0 1 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtggctccatgagcagtggtaattactgc tggggctggggccgccagcccccaggaaaggggctggagtggattggaagt atgtgttatggtgggagcacc tactacagcttgtcccccaagggtcgagtcaccatatccatagactcgtcgaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggctgtgtattactgt gcgagacgcgcattagacagtagtggtcattacattgacatc ARRALDSSGHYIDI IGHV4-39 IGHD3-22 IGHJ4 ggtggctccatgagcagtggtaattactgc tggggctggggccgccagcccccaggaaaggggctggagtggattggaagt atgtgttatggtgggagcacc tactacagcttgtcccccaagggtcgagtcaccatatccatagactcgtcgaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggctgtgtattactgt gcgagacgcgcattagacagtagtggtcattacattgacatc IGA1 19 216 14 5 6 9 8 10 3 0 9 7 JY8QFUQ01CKFL6 18538 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 882 89.86 195/217 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-9*01 F 2 8 8 10 8.8.10 [1.17.38.11] CVSDLAGQKGSW in-frame + ctcaggattcaccttcagtaggtactggatgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgtattaatgaagacggcagccacacagatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagttgtatttgcaaatgaacagtctgagaggcgaggacacggctgtctattattgtgtaagcgatttggctggtcagaaaggttcgtggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [278] t78>a|c108>g,S36>R(- - -)|c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|c259>t|c265>t|c287>g,A96>G(- + -)|g303>c|c309>t|c314>t,A105>V(+ - +)|a318>c,R106>S(- - -)| t78>a| c108>g,S36>R(- - -)| c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|c259>t|c265>t|c287>g,A96>G(- + -)|g303>c|c309>t| c314>t,A105>V(+ - +)|a318>c,R106>S(- - -)| 320 225 201 24 8 16 2 3 6 4 2 1 0 2 2 2 0 0 78 4 3 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 49 2 0 2 0 0 1 1 0 0 0 0 0 0 0 0 30 24 16 8 3 5 1 2 0 2 1 0 0 1 1 0 0 0 117 114 104 10 4 6 1 1 4 1 0 1 0 0 1 1 0 0 8 8 6 2 0 2 0 0 1 0 1 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctca ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagccacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagttgtatttgcaaatgaacagtctgagaggcgaggacacggctgtctattattgt gtaagcgatttggctggtcagaaaggttcg VSDLAGQKGS IGHV3-74 IGHD3-9 IGHJ4 ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagccacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagttgtatttgcaaatgaacagtctgagaggcgaggacacggctgtctattattgt gtaagcgatttggctggtcagaaaggttcg IGA1 21 213 14 7 8 13 6 8 4 3 8 6 JY8QFUQ01CKI1Y 9935 productive Homsap IGHV1-2*02 F 1029 98.13 210/214 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 192 86.27 44/51 nt Homsap IGHD3-3*01 F 2 8 8 16 8.8.16 [0.17.38.11] CARAIDFGSGDKRGFDSW in-frame - Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) tggatacaccttcaccggctactctatacactgggtgcgacaggcccctggacaagggcttgagtggatgggatggatcaaccctaacagtggtggcacaaactatgcacagaaatttcagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggagctgagcaggctgacatctgacgacacggccgtgtattactgtgcgagagccatcgattttgggagtggtgacaaacgggggtttgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [293, 292] a113>c,Y38>S(+ - -)|g117>a,M39>I(+ + -)|g210>a|g284>c,R95>T(- - -)| a113>c,Y38>S(+ - -)| g117>a,M39>I(+ + -)| g210>a|g284>c,R95>T(- - -)| a320>c| 319 (320) 221 (222) 217 4 (5) 1 (2) 3 0 2 0 0 1 (2) 0 0 0 1 0 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 112 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatacaccttcaccggctactct atacactgggtgcgacaggcccctggacaagggcttgagtggatgggatgg atcaaccctaacagtggtggcaca aactatgcacagaaatttcagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggagctgagcaggctgacatctgacgacacggccgtgtattactgt gcgagagccatcgattttgggagtggtgacaaacgggggtttgactcc ARAIDFGSGDKRGFDS IGHV1-2 IGHD3-3 IGHJ5 ggatacaccttcaccggctactct atacactgggtgcgacaggcccctggacaagggcttgagtggatgggatgg atcaaccctaacagtggtggcaca aactatgcacagaaatttcagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggagctgagcaggctgacatctgacgacacggccgtgtattactgt gcgagagccatcgattttgggagtggtgacaaacgggggtttgactcc IGA2 4 213 2 2 2 3 0 1 1 0 2 1 JY8QFUQ01CKO1P 10760 productive (see comment) Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F (see comment) 809 84.82 190/224 nt 83.93 188/224 nt Homsap IGHJ5*02 F 156 78.43 40/51 nt Homsap IGHD3-16*01 F 2 8 10 13 8.10.13 [2.16.38.11] CSTGGPNGYESTYPW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (84,82% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR1-IMGT at codon 28 of V-REGION: 'G' (1 nucleotides at position 12 to 12 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 46 of V-REGION: 1 nucleotides (from position 54 in the user submitted sequence), (cause frameshift) gcctctggattGcaccttcaatatcaagtggatgagttgggtccgccaggctccgggaaggggctggagtgggttggccgcatcaagagcacctctgatggtgggacaacagactccattgcacacgtcaaagacagattcatcatctcaagagatgattcaagaaatacactgtacttacaaatgaacaacctgagagtcgaggatacaggcgtctattattgttccacaggagggccaaatggttatgagtctacctatccctggggcccgggaaccctggtcaccgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [295] t87>c|g104>a,S35>N(- - -)|a107>t,N36>I(- - -)|g109>a,A37>K(- - -)|c110>a,A37>K(- - -)|c111>g,A37>K(- - -)|c120>t|t165>c|t168>c|a171>g|a176>c,K59>T(- - -)|a177>c,K59>T(- - -)|a178>t,T60>S(+ - +)|a200>c,Y67>S(+ - -)|g202>a,A68>I(+ - +)|c203>t,A68>I(+ - +)|c209>a,P70>H(+ - -)|g213>c|g221>a,G74>D(- - -)|c230>t,T77>I(- - -)|a251>g,K84>R(+ + +)|c255>t|g258>a|t264>c|c265>t|g267>a|g278>a,S93>N(- - -)|a284>g,K95>R(+ + +)|a286>g,T96>V(- - -)|c287>t,T96>V(- - -)|c294>t|c299>g,A100>G(- + -)|g303>c|c309>t|a313>t,T105>S(+ - +)| t87>c|g104>a,S35>N(- - -)|a107>t,N36>I(- - -)|g109>a,A37>K(- - -)|c110>a,A37>K(- - -)|c111>g,A37>K(- - -)| c120>t|t165>c| t168>c|a171>g|a176>c,K59>T(- - -)|a177>c,K59>T(- - -)|a178>t,T60>S(+ - +)| a200>c,Y67>S(+ - -)|g202>a,A68>I(+ - +)|c203>t,A68>I(+ - +)|c209>a,P70>H(+ - -)|g213>c|g221>a,G74>D(- - -)|c230>t,T77>I(- - -)|a251>g,K84>R(+ + +)|c255>t|g258>a|t264>c|c265>t|g267>a|g278>a,S93>N(- - -)|a284>g,K95>R(+ + +)|a286>g,T96>V(- - -)|c287>t,T96>V(- - -)|c294>t|c299>g,A100>G(- + -)|g303>c|c309>t| a313>t,T105>S(+ - +)|a320>g| 319 (320) 231 (232) 196 35 (36) 14 (15) 21 4 (5) 7 8 4 3 2 3 0 2 2 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 1 5 0 2 0 1 0 1 1 0 0 1 0 0 51 50 48 2 2 0 0 0 1 1 0 0 0 0 0 0 0 0 30 30 25 5 2 3 1 0 0 1 2 0 1 0 0 0 0 0 117 114 93 21 9 12 3 5 7 1 1 1 0 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 0 0 0 0 1 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| gcctct ggattcaccttcaatatcaagtgg atgagttgggtccgccaggctccgggaaggggctggagtgggttggccgc atcaagagcacctctgatggtgggacaaca gactccattgcacacgtcaaagacagattcatcatctcaagagatgattcaagaaatacactgtacttacaaatgaacaacctgagagtcgaggatacaggcgtctattattgt tccacaggagggccaaatggttatgagtctacctatccc STGGPNGYESTYP IGHV3-15 IGHD3-16 IGHJ5 ggattcaccttcaatatcaagtgg atgagttgggtccgccaggctccgggaaggggctggagtgggttggccgc atcaagagcacctctgatggtgggacaaca gactccattgcacacgtcaaagacagattcatcatctcaagagatgattcaagaaatacactgtacttacaaatgaacaacctgagagtcgaggatacaggcgtctattattgt tccacaggagggccaaatggttatgagtctacctatccc IGA1 34 218 23 11 15 21 8 13 11 3 12 8 JY8QFUQ01CKPJN 6060 productive Homsap IGHV3-9*01 F 990 95.39 207/217 nt NA Homsap IGHJ4*03 F 213 93.75 45/48 nt Homsap IGHD1-26*01 F 1 8 8 11 8.8.11 [1.17.38.11] CAKEGNLGYFDDW in-frame + ctctggattcaggtttgatgattatgccatgcactgggtccggcaagctccagggaagggcctggagtggatctcaggtattagctggaatagtggtagtatagggtatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaaagaaggtaatctgggctactttgacgactggggtcaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [281] c86>g,T29>R(- - -)|c87>g,T29>R(- - -)|g157>a,V53>I(+ - +)|t171>c|c192>t|c198>g|t279>c|t288>c|t301>g,L101>V(+ - +)|g303>a,L101>V(+ - +)|a315>g| c86>g,T29>R(- - -)|c87>g,T29>R(- - -)| g157>a,V53>I(+ - +)| t171>c|c192>t| c198>g|t279>c|t288>c|t301>g,L101>V(+ - +)|g303>a,L101>V(+ - +)| a315>g|t321>a,D107>E(+ - +)|a322>g| 320 (322) 225 (227) 214 11 (13) 6 (7) 5 (6) 1 (2) 2 1 3 0 0 0 0 (1) 0 3 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 0 0 0 2 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 22 2 2 0 0 0 1 1 0 0 0 0 0 0 0 0 117 114 109 5 3 2 0 1 0 2 0 0 0 0 0 1 0 1 8 (10) 8 7 1 (3) 1 (2) 0 (1) 1 (2) 0 0 0 0 0 0 0 (1) 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaggtttgatgattatgcc atgcactgggtccggcaagctccagggaagggcctggagtggatctcaggt attagctggaatagtggtagtata gggtatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaagaaggtaatctgggctactttgacgac AKEGNLGYFDD IGHV3-9 IGHD1-26 IGHJ4 ggattcaggtttgatgattatgcc atgcactgggtccggcaagctccagggaagggcctggagtggatctcaggt attagctggaatagtggtagtata gggtatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaagaaggtaatctgggctactttgacgac IGA1 10 213 6 4 3 6 3 4 3 2 3 2 JY8QFUQ01CKX6X 20303 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 889 91.08 194/213 nt 90.61 193/213 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD4-23*01 ORF 3 8 6 12 8.6.12 [1.17.38.11] CASSVFSPGGFDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 56 of V-REGION: 1 nucleotides (from position 80 in the user submitted sequence), (cause frameshift) ttcggggttcaccgtcagtagcaagtacatgacctgggtccgccaggctccggggaagggactggagtctgtctctgtttttatagcggtgatcaaacatactacgcagactccgtgaggggccgattcaccatctccatagacaattccaagaacacactgtatcttcaaatgaacggcctgcgagccgaggacacggccgtgtattattgtgcgagctccgtgtttagtcccggaggctttgactactggggccagggaaccctggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcgtcgcatgc NA IGA2 86 95 [280, 281] c75>t|t78>g|c111>g,N37>K(+ - -)|g119>c,S40>T(+ - +)|a138>g|g147>a|g155>c,W52>S(- - -)|g156>t,W52>S(- - -)|a162>t|g188>a,G63>D(- - -)|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>a,S64>Q(- - -)|a215>g,K72>R(+ + +)|g239>t,R80>I(- + -)|g258>a|a277>g,S93>G(+ + -)|a283>c|c309>t| c75>t|t78>g| c111>g,N37>K(+ - -)| g119>c,S40>T(+ - +)|a138>g|g147>a|g155>c,W52>S(- - -)|g156>t,W52>S(- - -)|a162>t| g188>a,G63>D(- - -)|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>a,S64>Q(- - -)| a215>g,K72>R(+ + +)|g239>t,R80>I(- + -)|g258>a|a277>g,S93>G(+ + -)|a283>c|c309>t| a318>c,R106>S(- - -)|g319>t|a320>c| 317 (320) 218 (221) 199 19 (22) 8 (10) 11 (12) 3 4 2 0 2 (4) 1 1 0 2 1 2 (3) 1 78 4 2 2 2 0 0 0 1 0 0 0 0 0 0 0 0 1 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 45 6 3 3 1 1 0 0 0 0 1 0 2 0 1 0 30 20 16 4 0 4 0 2 0 0 1 1 0 0 0 0 0 0 117 114 108 6 3 3 2 1 1 0 1 0 0 0 0 0 1 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 0 0 0 0 (2) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ttcg gggttcaccgtcagtagcaagtac atgacctgggtccgccaggctccggggaagggactggagtctgtctctgtt tttatagcggtgatcaaaca tactacgcagactccgtgaggggccgattcaccatctccatagacaattccaagaacacactgtatcttcaaatgaacggcctgcgagccgaggacacggccgtgtattattgt gcgagctccgtgtttagtcccggaggctttgactac ASSVFSPGGFDY IGHV3-53 IGHD4-23 IGHJ4 gggttcaccgtcagtagcaagtac atgacctgggtccgccaggctccggggaagggactggagtctgtctctgtt tttatagcggtgatcaaaca tactacgcagactccgtgaggggccgattcaccatctccatagacaattccaagaacacactgtatcttcaaatgaacggcctgcgagccgaggacacggccgtgtattattgt gcgagctccgtgtttagtcccggaggctttgactac IGA2 17 209 8 9 5 11 3 6 6 0 6 5 JY8QFUQ01CL7VE 10341 productive (see comment) Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F 858 89.25 191/214 nt 88.79 190/214 nt Homsap IGHJ3*02 F (see comment) 196 88 44/50 nt Homsap IGHD6-13*01 F 1 8 7 14 8.7.14 [1.16.38.11] CARPTYSSGRDAFDTW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 59 in the user submitted sequence), (cause frameshift) tctatggtgggtccttcagtacttactactggacatggatccgccagcacccagagaaggactggagtggattggggaaatcaatcacagtggaagccccaactacagcccgtccctcaagagtcgagtcctcatatcgatagacacgtccaagaatcaggtctccctcaacctcttctctgtgaccgccgcggacacgggtgtgtattattgtgcgaggcccacatatagcagcggccgggacgcttttgatacctggggccaagggacgacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [287] g106>a,G36>T(+ - -)|g107>c,G36>T(+ - -)|g119>c,S40>T(+ - +)|c120>a,S40>T(+ - +)|c134>a,P45>H(+ - -)|g140>a,G47>E(- - -)|g147>a|t174>c|a193>c,T65>P(+ + -)|a203>g,N68>S(- - -)|a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|a237>g|g238>a,V80>I(+ - +)|c255>t|t259>g,F87>V(+ - -)|g267>c|g270>c,K90>N(+ - -)|g273>c|a274>t,S92>F(- - -)|g275>t,S92>F(- - -)|c299>g,A100>G(- + -)|c309>t| g106>a,G36>T(+ - -)|g107>c,G36>T(+ - -)| g119>c,S40>T(+ - +)|c120>a,S40>T(+ - +)|c134>a,P45>H(+ - -)|g140>a,G47>E(- - -)|g147>a| t174>c|a193>c,T65>P(+ + -)| a203>g,N68>S(- - -)|a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|a237>g|g238>a,V80>I(+ - +)|c255>t|t259>g,F87>V(+ - -)|g267>c|g270>c,K90>N(+ - -)|g273>c|a274>t,S92>F(- - -)|g275>t,S92>F(- - -)|c299>g,A100>G(- + -)|c309>t| a318>g|g319>c|g320>c| 317 (320) 219 (222) 196 23 (26) 7 (10) 16 2 (3) 4 3 1 2 2 1 0 5 (7) 1 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 51 50 45 5 1 4 0 2 0 0 0 2 0 0 1 0 0 0 30 21 19 2 1 1 0 0 0 1 1 0 0 0 0 0 0 0 117 114 100 14 5 9 2 1 3 0 1 0 1 0 3 1 1 1 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 0 0 0 0 0 0 0 (2) 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctat ggtgggtccttcagtacttactac tggacatggatccgccagcacccagagaaggactggagtggattggggaa atcaatcacagtggaagcccc aactacagcccgtccctcaagagtcgagtcctcatatcgatagacacgtccaagaatcaggtctccctcaacctcttctctgtgaccgccgcggacacgggtgtgtattattgt gcgaggcccacatatagcagcggccgggacgcttttgatacc ARPTYSSGRDAFDT IGHV4-34 IGHD6-13 IGHJ3 ggtgggtccttcagtacttactac tggacatggatccgccagcacccagagaaggactggagtggattggggaa atcaatcacagtggaagcccc aactacagcccgtccctcaagagtcgagtcctcatatcgatagacacgtccaagaatcaggtctccctcaacctcttctctgtgaccgccgcggacacgggtgtgtattattgt gcgaggcccacatatagcagcggccgggacgcttttgatacc IGA1 23 209 10 13 7 16 3 7 6 1 13 3 JY8QFUQ01CLEAH 6208 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 882 89.86 195/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 2 8 8 17 8.8.17 [1.17.38.11] CARGEFYYETSGRRGFDYW in-frame - ctctggattcaccttcactacctcctggatgcactgggtccgccaagctccagggaaggggctaatgtgggtcgcacgtattaataaggatggcagtagtacaagttatgaggactccgtgaagggccgattcaccatctccagagacaacgccaagaccacactgtacttggaaatggacagtctgagagtcgaggacacggctatgtattattgtgcaagaggggaattttactatgaaactagtggtcgaaggggttttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [299, 298] g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|g150>a|g151>a,V51>M(+ - -)|t160>g,S54>A(- + -)|g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g186>c|c192>t|c198>t|c201>t|c203>a,A68>E(- - -)|a254>c,N85>T(- + -)|g258>a|t264>c|c265>t|c268>g,Q90>E(+ + -)|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g301>a,V101>M(+ - -)|c309>t| g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)| g150>a|g151>a,V51>M(+ - -)|t160>g,S54>A(- + -)| g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g186>c|c192>t| c198>t|c201>t|c203>a,A68>E(- - -)|a254>c,N85>T(- + -)|g258>a|t264>c|c265>t|c268>g,Q90>E(+ + -)|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g301>a,V101>M(+ - -)|c309>t| a320>g| 319 (320) 224 (225) 202 22 (23) 9 (10) 13 1 (2) 5 6 1 2 1 0 0 3 1 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 0 1 0 0 0 2 0 0 0 51 51 48 3 1 2 0 2 0 0 0 0 0 0 0 0 0 1 30 24 20 4 2 2 0 1 1 0 0 0 0 0 1 0 0 1 117 114 102 12 6 6 1 2 5 1 1 1 0 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcactacctcctgg atgcactgggtccgccaagctccagggaaggggctaatgtgggtcgcacgt attaataaggatggcagtagtaca agttatgaggactccgtgaagggccgattcaccatctccagagacaacgccaagaccacactgtacttggaaatggacagtctgagagtcgaggacacggctatgtattattgt gcaagaggggaattttactatgaaactagtggtcgaaggggttttgactac ARGEFYYETSGRRGFDY IGHV3-74 IGHD3-22 IGHJ4 ggattcaccttcactacctcctgg atgcactgggtccgccaagctccagggaaggggctaatgtgggtcgcacgt attaataaggatggcagtagtaca agttatgaggactccgtgaagggccgattcaccatctccagagacaacgccaagaccacactgtacttggaaatggacagtctgagagtcgaggacacggctatgtattattgt gcaagaggggaattttactatgaaactagtggtcgaaggggttttgactac IGA2 22 213 13 9 11 16 2 6 7 2 8 5 JY8QFUQ01CLIT1 13006 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 827 87.44 188/215 nt 86.98 187/215 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-21*02 F 3 8 7 15 8.7.15 [1.17.38.11] CARGRTVVTVIDYIDYW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'A' (1 nucleotides at position 11 to 11 in the user submitted sequence), (cause frameshift); tctctgggggActccattagtggttactattggacgtggatccggcagaccccagggaagggactggagtggattggaaatgtccattacactgggagtaccaagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgtgcgagaggacgaacggtggtgactgttattgactacattgactattggggtcagggagccctggtcagcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [292] t81>g|c90>t|a106>g,S36>G(+ + -)|c114>t|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>a,P45>T(+ + -)|g162>a|t163>a,Y55>N(- - -)|a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|c192>t|c198>g,N66>K(+ - -)|a203>g,N68>S(- - -)|a234>g,I78>M(+ + -)|a240>t|c245>t,T82>M(- - -)|a251>g,K84>R(+ + +)|t262>a,S88>T(+ - +)|g267>c|g270>a|c271>t|g275>c,S92>T(+ - +)|g282>a|a283>g,T95>A(- - -)|g303>c| t81>g|c90>t|a106>g,S36>G(+ + -)|c114>t| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>a,P45>T(+ + -)|g162>a|t163>a,Y55>N(- - -)| a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|c192>t| c198>g,N66>K(+ - -)|a203>g,N68>S(- - -)|a234>g,I78>M(+ + -)|a240>t|c245>t,T82>M(- - -)|a251>g,K84>R(+ + +)|t262>a,S88>T(+ - +)|g267>c|g270>a|c271>t|g275>c,S92>T(+ - +)|g282>a|a283>g,T95>A(- - -)|g303>c| a320>g| 319 (320) 222 (223) 195 27 (28) 11 (12) 16 6 (7) 3 5 1 0 1 1 2 5 2 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 3 1 1 0 2 0 0 0 0 0 0 0 0 1 51 51 46 5 1 4 0 1 0 0 0 1 0 1 1 1 0 0 30 21 17 4 1 3 1 0 1 1 0 0 0 0 1 0 0 0 117 114 100 14 6 8 4 2 2 0 0 0 1 1 3 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct gggggctccattagtggttactat tggacgtggatccggcagaccccagggaagggactggagtggattggaaat gtccattacactgggagtacc aagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttattgactacattgactat ARGRTVVTVIDYIDY IGHV4-59 IGHD2-21 IGHJ4 gggggctccattagtggttactat tggacgtggatccggcagaccccagggaagggactggagtggattggaaat gtccattacactgggagtacc aagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttattgactacattgactat IGA1 27 210 15 12 8 16 7 11 7 4 12 4 JY8QFUQ01CLMWX 7199 productive (see comment) Homsap IGHV3-9*01 F 896 90.37 197/218 nt 89.91 196/218 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-22*01 F 2 8 8 13 8.8.13 [1.17.38.11] CAKDGDSGWDYYDHW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'T' (1 nucleotides at position 10 to 10 in the user submitted sequence), (cause frameshift); cctctggatTtaacctttgatcaatatgccatgtattgggtccggcaagctccagggaagggcctggagtgggtctccggtatcactgggaatagtggttccataggctatgcggactctgtgaggggccgattcaccatctccagagacaacgccaagaagtcactatatttggaaatgaatagtctgagtgttgaggacacggccttgtatttctgtgcaaaggatggcgacagcggttgggattattatgaccactggggcccgggaactctggtcaccgtctcgtcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG4 100 88 [290] c84>a,F28>L(+ - -)|g106>c,D36>Q(+ - -)|t108>a,D36>Q(+ - -)|c118>t,H40>Y(+ - -)|c120>t,H40>Y(+ - -)|a162>c|t168>c|g170>c,S57>T(+ - +)|t172>g,W58>G(- - -)|a190>t|g191>c|a215>g,K72>R(+ + +)|c255>g,N85>K(+ - -)|c258>a|g261>a|c265>t|c268>g,Q90>E(+ + -)|c276>t|a285>t,R95>S(- - -)|c287>t,A96>V(+ - +)|a308>t,Y103>F(- + -)|a318>g| c84>a,F28>L(+ - -)|g106>c,D36>Q(+ - -)|t108>a,D36>Q(+ - -)| c118>t,H40>Y(+ - -)|c120>t,H40>Y(+ - -)|a162>c| t168>c|g170>c,S57>T(+ - +)|t172>g,W58>G(- - -)|a190>t|g191>c| a215>g,K72>R(+ + +)|c255>g,N85>K(+ - -)|c258>a|g261>a|c265>t|c268>g,Q90>E(+ + -)|c276>t|a285>t,R95>S(- - -)|c287>t,A96>V(+ - +)|a308>t,Y103>F(- + -)| a318>g|a322>g| 321 (322) 227 (228) 205 22 (23) 9 (10) 13 2 (3) 1 5 1 1 2 3 1 3 2 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 0 0 1 0 1 1 0 0 0 51 51 48 3 1 2 0 0 2 0 1 0 0 0 0 0 0 0 30 24 19 5 3 2 0 0 0 1 0 0 1 0 2 0 0 1 117 114 104 10 4 6 1 1 3 0 0 1 2 0 0 2 0 0 9 (10) 9 8 1 (2) 1 (2) 0 1 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattaacctttgatcaatatgcc atgtattgggtccggcaagctccagggaagggcctggagtgggtctccggt atcactgggaatagtggttccata ggctatgcggactctgtgaggggccgattcaccatctccagagacaacgccaagaagtcactatatttggaaatgaatagtctgagtgttgaggacacggccttgtatttctgt gcaaaggatggcgacagcggttgggattattatgaccac AKDGDSGWDYYDH IGHV3-9 IGHD3-22 IGHJ4 ggattaacctttgatcaatatgcc atgtattgggtccggcaagctccagggaagggcctggagtgggtctccggt atcactgggaatagtggttccata ggctatgcggactctgtgaggggccgattcaccatctccagagacaacgccaagaagtcactatatttggaaatgaatagtctgagtgttgaggacacggccttgtatttctgt gcaaaggatggcgacagcggttgggattattatgaccac IGG4 21 213 8 13 6 13 2 8 5 3 8 5 JY8QFUQ01CLR99 22222 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 948 93.93 201/214 nt 93.46 200/214 nt Homsap IGHJ4*03 F 213 93.75 45/48 nt Homsap IGHD1-26*01 F 1 8 7 11 8.7.11 [1.17.38.11] CAKEGNLGYFDDW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 57 of V-REGION: 3 nucleotides (from position 83 in the user submitted sequence), (do not cause frameshift) ctctggattcacctttagcagccatcccatgagctgggtccgccaggctccgggaaaagggctggagtggatctcagctttcgttcgtagtggtaacacatactacgtagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaaagaaggtaatctgggctactttgacgactggggtcaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [278] t109>c,Y37>H(+ - -)|g112>c,A38>P(- - -)|a138>g|g141>a|g144>a|g157>a,V53>I(+ - +)|a166>t,I56>F(+ - -)|t168>c,I56>F(+ - -)|g173>t,G58>V(- - -)|a175>c,S59>R(- - -)|g184>a,G62>S(+ + -)|g191>a,S64>N(- - -)|c203>t,A68>V(+ - +)| t109>c,Y37>H(+ - -)|g112>c,A38>P(- - -)| a138>g|g141>a|g144>a|g157>a,V53>I(+ - +)| a166>t,I56>F(+ - -)|t168>c,I56>F(+ - -)|g173>t,G58>V(- - -)|a175>c,S59>R(- - -)|g184>a,G62>S(+ + -)|g191>a,S64>N(- - -)| c203>t,A68>V(+ - +)| 320 222 209 13 3 10 1 5 1 2 1 0 1 0 1 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 1 0 0 0 0 1 0 0 0 51 51 47 4 3 1 1 3 0 0 0 0 0 0 0 0 0 0 30 21 15 6 0 6 0 2 0 1 1 0 1 0 0 0 1 0 117 114 113 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcagccatccc atgagctgggtccgccaggctccgggaaaagggctggagtggatctcagct ttcgttcgtagtggtaacaca tactacgtagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaagaaggtaatctgggctactttgacgac AKEGNLGYFDD IGHV3-23 IGHD1-26 IGHJ4 ggattcacctttagcagccatccc atgagctgggtccgccaggctccgggaaaagggctggagtggatctcagct ttcgttcgtagtggtaacaca tactacgtagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaagaaggtaatctgggctactttgacgac IGA1 13 210 9 4 6 8 3 5 3 0 2 8 JY8QFUQ01CLXOH 8097 productive Homsap IGHV3-11*01 F 968 94.04 205/218 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 174 82.35 42/51 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [1.17.38.11] CARDPHRGDGCTYDSW in-frame - Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) cctctggattcaccttcagtgactaccacatgggctggatccgccaggctccagggaaggggctggagtgcgtttcatacattagcactagtggtcgtgacatatacaacgcagactctgtgaagggccgattcaccatctccagggacaacgcccagaagtcactgtatctgcaaatgaacagcctgagagccgaggacacagccgtgtatttctgtgcgagggatccccatcgtggagatggctgcacttacgactcctggggccagggaaccctggtcaccgtctcctccgcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [291] t112>c,Y38>H(+ - -)|a118>g,S40>G(+ + -)|g156>c,W52>C(+ - -)|t171>c|g173>c,S58>T(+ - +)|a187>c,S63>R(- - -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|t199>a,Y67>N(- - -)|a250>c,K84>Q(+ - -)|c255>g,N85>K(+ - -)|g297>a|a308>t,Y103>F(- + -)|a318>g| t112>c,Y38>H(+ - -)| a118>g,S40>G(+ + -)|g156>c,W52>C(+ - -)| t171>c|g173>c,S58>T(+ - +)|a187>c,S63>R(- - -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)| t199>a,Y67>N(- - -)|a250>c,K84>Q(+ - -)|c255>g,N85>K(+ - -)|g297>a|a308>t,Y103>F(- + -)| a318>g| 320 226 212 14 3 11 3 1 0 2 2 1 1 1 2 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 51 51 49 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 30 24 19 5 1 4 1 0 0 1 1 1 0 0 1 0 0 0 117 114 109 5 1 4 0 1 0 0 1 0 1 1 0 1 0 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtgactaccac atgggctggatccgccaggctccagggaaggggctggagtgcgtttcatac attagcactagtggtcgtgacata tacaacgcagactctgtgaagggccgattcaccatctccagggacaacgcccagaagtcactgtatctgcaaatgaacagcctgagagccgaggacacagccgtgtatttctgt gcgagggatccccatcgtggagatggctgcacttacgactcc ARDPHRGDGCTYDS IGHV3-11 IGHD5-24 IGHJ5 ggattcaccttcagtgactaccac atgggctggatccgccaggctccagggaaggggctggagtgcgtttcatac attagcactagtggtcgtgacata tacaacgcagactctgtgaagggccgattcaccatctccagggacaacgcccagaagtcactgtatctgcaaatgaacagcctgagagccgaggacacagccgtgtatttctgt gcgagggatccccatcgtggagatggctgcacttacgactcc IGA2 13 213 5 8 1 5 4 8 1 1 6 5 JY8QFUQ01CM33P 13316 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 979 93.69 208/222 nt 93.24 207/222 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-19*01 F 1 10 7 13 10.7.13 [2.17.38.11] CARHGSGWLQELDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 68 to 68 in the user submitted sequence), (cause frameshift); gtctctggtggctccatcatcagagacagtgcctactggggctggatccgccagcccccagggaaggGggctggagtggcttgggagcatctattatagtgggagtacctactacaatccctccctcaagagtcgagtcaccatatccgtagacacgtccaagaagcagttctccctgaagctgagctctgtgaccgccgcagacacggctgtatattactgtgcgagacatggcagtggctggctccaggaacttgactactggggccagggaaccctggtcaccgtctccacagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [293] g92>t,S31>I(- - -)|t102>a,S34>R(- - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|t105>c,S35>D(- - -)|t109>g,Y37>A(- - -)|a110>c,Y37>A(- - -)|a157>c,I53>L(+ + +)|t165>c|c192>t|c204>t|g207>c|c255>g,N85>K(+ - -)|g303>a| g92>t,S31>I(- - -)|t102>a,S34>R(- - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|t105>c,S35>D(- - -)|t109>g,Y37>A(- - -)|a110>c,Y37>A(- - -)| a157>c,I53>L(+ + +)|t165>c| c192>t| c204>t|g207>c|c255>g,N85>K(+ - -)|g303>a| 320 230 216 14 5 9 1 2 2 2 2 0 0 1 1 1 1 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 23 7 0 7 1 1 0 1 1 0 0 1 0 0 1 1 51 51 49 2 1 1 0 0 0 1 1 0 0 0 0 0 0 0 30 21 20 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 117 114 110 4 3 1 0 1 1 0 0 0 0 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct ggtggctccatcatcagagacagtgcctac tggggctggatccgccagcccccagggaaggggctggagtggcttgggagc atctattatagtgggagtacc tactacaatccctccctcaagagtcgagtcaccatatccgtagacacgtccaagaagcagttctccctgaagctgagctctgtgaccgccgcagacacggctgtatattactgt gcgagacatggcagtggctggctccaggaacttgactac ARHGSGWLQELDY IGHV4-39 IGHD6-19 IGHJ4 ggtggctccatcatcagagacagtgcctac tggggctggatccgccagcccccagggaaggggctggagtggcttgggagc atctattatagtgggagtacc tactacaatccctccctcaagagtcgagtcaccatatccgtagacacgtccaagaagcagttctccctgaagctgagctctgtgaccgccgcagacacggctgtatattactgt gcgagacatggcagtggctggctccaggaacttgactac IGA2 14 216 7 7 4 7 3 7 4 1 2 7 JY8QFUQ01CM5UQ 8282 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 990 95.39 207/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 8 18 8.8.18 [1.17.38.11] CARAGYCNNGVCYSSYFDFW in-frame + ctctggattcaccttcagtagctactggatgcactgggtccgccaagctccggggaaggggctggtgtgggtctcacgtatgaatagtgatggcagtgacataaggtacgcggactccgtgaggggccgattcaccatctccagagacaacaccaagaacacgctgtatctacaaatgaacagtctgagagccgaggacacggctgtgtattactgtgcaagagcgggatattgtaataatggtgtatgctactccagttactttgacttctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtcagggcttcttccc NA IGA1 100 100 [302] a138>g|t168>g,I56>M(+ + -)|g186>c|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>t,T65>I(- - -)|c198>g,S66>R(- - -)|a215>g,K72>R(+ + +)|g247>a,A83>T(- - -)|g267>a| a138>g| t168>g,I56>M(+ + -)|g186>c|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>t,T65>I(- - -)| c198>g,S66>R(- - -)|a215>g,K72>R(+ + +)|g247>a,A83>T(- - -)|g267>a| a320>c| 319 (320) 224 (225) 214 10 (11) 3 (4) 7 3 3 1 0 0 (1) 0 0 0 1 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 30 24 19 5 1 4 1 1 1 0 0 0 0 0 1 0 0 1 117 114 110 4 1 3 1 2 0 0 0 0 0 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccggggaaggggctggtgtgggtctcacgt atgaatagtgatggcagtgacata aggtacgcggactccgtgaggggccgattcaccatctccagagacaacaccaagaacacgctgtatctacaaatgaacagtctgagagccgaggacacggctgtgtattactgt gcaagagcgggatattgtaataatggtgtatgctactccagttactttgacttc ARAGYCNNGVCYSSYFDF IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccggggaaggggctggtgtgggtctcacgt atgaatagtgatggcagtgacata aggtacgcggactccgtgaggggccgattcaccatctccagagacaacaccaagaacacgctgtatctacaaatgaacagtctgagagccgaggacacggctgtgtattactgt gcaagagcgggatattgtaataatggtgtatgctactccagttactttgacttc IGA1 10 213 7 3 4 6 3 4 2 1 3 4 JY8QFUQ01CN7M5 16779 productive Homsap IGHV3-11*04 F, or Homsap IGHV3-48*01 F or Homsap IGHV3-48*02 F 972 92.48 209/226 nt NA Homsap IGHJ6*02 F 256 90.32 56/62 nt Homsap IGHD4-23*01 ORF 2 8 8 20 8.8.20 [4.17.38.11] CVRGDYGGAPGHYYDPYGLDVW in-frame + ctctgcagcctctggattcattgtcaatagcaactacatgagttgggtccgccaggctccagggaaggggctggagtggatttcatacattagtagtagtggtattatcatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgtgtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtctggggccaagggaccacggtcaaagtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttgccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [318] g68>c,C23>S(- - -)|c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|t88>g,F30>V(+ - -)|g104>a,S35>N(- - -)|g106>a,D36>S(- - -)|a107>g,D36>S(- - -)|t109>a,Y37>N(- - -)|c120>t|a124>g,I42>V(+ - +)|g157>a,V53>I(+ - +)|g188>t,S63>I(- - -)|c191>t,T64>I(- - -)|g240>a|c246>t|c287>t,A96>V(+ - +)|c309>t|c314>t,A105>V(+ - +)| g68>c,C23>S(- - -)| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|t88>g,F30>V(+ - -)|g104>a,S35>N(- - -)|g106>a,D36>S(- - -)|a107>g,D36>S(- - -)|t109>a,Y37>N(- - -)| c120>t|a124>g,I42>V(+ - +)|g157>a,V53>I(+ - +)| g188>t,S63>I(- - -)|c191>t,T64>I(- - -)| g240>a|c246>t|c287>t,A96>V(+ - +)|c309>t| c314>t,A105>V(+ - +)|a320>g| 319 (320) 233 (234) 215 18 (19) 4 (5) 14 2 (3) 4 8 0 0 0 0 1 1 0 1 1 78 13 12 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 36 24 17 7 0 7 1 2 2 0 0 0 0 1 0 0 0 1 51 51 48 3 1 2 1 1 1 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 1 0 0 0 0 0 0 0 1 0 117 114 110 4 3 1 0 1 3 0 0 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctctgcagcctct ggattcattgtcaatagcaactac atgagttgggtccgccaggctccagggaaggggctggagtggatttcatac attagtagtagtggtattatcata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgt gtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtc VRGDYGGAPGHYYDPYGLDV IGHV3-11 IGHD4-23 IGHJ6 ggattcattgtcaatagcaactac atgagttgggtccgccaggctccagggaaggggctggagtggatttcatac attagtagtagtggtattatcata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgt gtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtc IGG1 16 213 13 3 11 12 2 4 4 0 3 9 JY8QFUQ01CNEDF 10865 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD4-17*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAGHLLGDPFDYW in-frame + ttctggagacaactttagcagatactggatcggctgggtccgccagatgcccgggaaaggcctggagtggatggggatcatctatcctggtgactctgacaccagatacagtccgtccttccaaggccaggtcaccatctcagccgacaagtccaccagtaccgcctacctgcagtggagcagtctgaaggtctcggacaccgccacgtattactgtgcgggacatcttctcggtgacccttttgactactggggccagggaaccccggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [282] t82>g,Y28>D(- - -)|g86>a,S29>N(- - -)|c104>g,T35>S(+ - +)|c108>a,S36>R(- - -)|g126>c|t192>c|c204>t|t251>c,I84>T(- - -)|c255>t|c279>t|c287>t,A96>V(+ - +)|t302>c,M101>T(- - -)|a316>g,R106>G(- - -)| t82>g,Y28>D(- - -)|g86>a,S29>N(- - -)|c104>g,T35>S(+ - +)|c108>a,S36>R(- - -)| g126>c| t192>c| c204>t|t251>c,I84>T(- - -)|c255>t|c279>t|c287>t,A96>V(+ - +)|t302>c,M101>T(- - -)| a316>g,R106>G(- - -)| 320 225 212 13 5 8 1 1 4 3 0 1 0 0 1 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 0 0 0 1 0 0 0 1 0 1 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 108 6 3 3 0 0 4 2 0 0 0 0 0 0 0 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ttct ggagacaactttagcagatactgg atcggctgggtccgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgacacc agatacagtccgtccttccaaggccaggtcaccatctcagccgacaagtccaccagtaccgcctacctgcagtggagcagtctgaaggtctcggacaccgccacgtattactgt gcgggacatcttctcggtgacccttttgactac AGHLLGDPFDY IGHV5-51 IGHD4-17 IGHJ4 ggagacaactttagcagatactgg atcggctgggtccgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgacacc agatacagtccgtccttccaaggccaggtcaccatctcagccgacaagtccaccagtaccgcctacctgcagtggagcagtctgaaggtctcggacaccgccacgtattactgt gcgggacatcttctcggtgacccttttgactac IGG2 12 213 8 4 5 8 3 4 4 1 3 4 JY8QFUQ01CNH24 10270 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 930 92.99 199/214 nt 91.59 196/214 nt Homsap IGHJ3*02 F 196 88 44/50 nt Homsap IGHD3-22*01 F 2 8 8 15 8.8.15 [0.17.38.11] CAARIDSGHYWNAFDIW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 41 of V-REGION: 'C' (1 nucleotides at position 33 to 33 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 46 of V-REGION: 'A' (1 nucleotides at position 48 to 48 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 74 of V-REGION: 'G' (1 nucleotides at position 125 to 125 in the user submitted sequence), (cause frameshift); tggatacagtttcaccaactactggatcggctCgggtgcgccagatgAcccgggaaaggcctggagtggatgggtatcatctatcctggtgactctgatacccggtacaacccgtccttccaagGgccaggtcaccttctccgtcgacaagtccatcaacaccgcctacctgcagtgggacagcctgaagacctcggacaccgccaagtattactgtgcggcgagaattgatagtggtcattactggaatgctttcgatatatggggccaagggacgatggtcaccgtcgcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [293] c87>t|t90>c|g107>a,S36>N(- - -)|g162>t|a196>c|a198>g|g203>a,S68>N(- - -)|a232>t,I78>F(+ - -)|a237>c|c239>t,A80>V(+ - +)|g254>a,S85>N(- - -)|a274>g,S92>D(- - -)|g275>a,S92>D(- - -)|g286>a,A96>T(- - -)|t302>a,M101>K(- + -)| c87>t|t90>c|g107>a,S36>N(- - -)| g162>t| a196>c|a198>g|g203>a,S68>N(- - -)|a232>t,I78>F(+ - -)|a237>c|c239>t,A80>V(+ - +)|g254>a,S85>N(- - -)|a274>g,S92>D(- - -)|g275>a,S92>D(- - -)|g286>a,A96>T(- - -)|t302>a,M101>K(- + -)| a316>g,R106>A(- - -)|g317>c,R106>A(- - -)|a318>g,R106>A(- - -)|c319>a|a320>g| 315 (320) 217 (222) 202 15 (20) 6 (8) 9 (12) 2 (5) 5 2 1 2 0 (1) 1 1 0 (1) 0 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 1 1 1 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 103 11 3 8 2 4 1 0 2 0 1 1 0 0 0 0 3 (8) 3 3 0 (5) 0 (2) 0 (3) 0 (3) 0 0 0 0 0 (1) 0 0 0 (1) 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacagtttcaccaactactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggtatc atctatcctggtgactctgatacc cggtacaacccgtccttccaaggccaggtcaccttctccgtcgacaagtccatcaacaccgcctacctgcagtgggacagcctgaagacctcggacaccgccaagtattactgt gcggcgagaattgatagtggtcattactggaatgctttcgatata AARIDSGHYWNAFDI IGHV5-51 IGHD3-22 IGHJ3 ggatacagtttcaccaactactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatgggtatc atctatcctggtgactctgatacc cggtacaacccgtccttccaaggccaggtcaccttctccgtcgacaagtccatcaacaccgcctacctgcagtgggacagcctgaagacctcggacaccgccaagtattactgt gcggcgagaattgatagtggtcattactggaatgctttcgatata IGA2 15 213 10 5 7 8 3 7 4 2 8 1 JY8QFUQ01CNLWR 19585 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 801 85.71 186/217 nt 85.25 185/217 nt Homsap IGHJ6*02 F (see comment) 193 79.03 49/62 nt Homsap IGHD6-19*01 F 1 8 8 17 8.8.17 [1.17.38.11] CVRDSDSSGYARYDSMDVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) in CDR2-IMGT at codon 58 of V-REGION: 'C' (1 nucleotides at position 86 to 86 in the user submitted sequence), (cause frameshift); ctctggattcaacctcaatacctttggcatgaactgggtccgccaggcgccagggaagggactggagtgggtctcacacgtcaatCgggggtagtactcacatatactacgcaggctcagtgaggggccggttcaccatctccagagacgacgccgggaactcagtctatctgcaaatgaatagcctgagagccgaggacacgggtttatattattgtgtgagagacagtgactcgtccggctacgcgaggtacgactctatggacgtctggggccaagggaccgcggtcaccgtctcatcagcctccaccaaggggcccatcggtcttcccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 94 [303, 302] c86>a,T29>N(- + -)|t88>c,F30>L(+ - -)|g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|a112>g,S38>G(+ + -)|t135>g|g147>a|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>a,S57>N(- - -)|a172>g,S58>G(+ + -)|t174>g,S58>G(+ + -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)|a206>g,D69>G(- - -)|a215>g,K72>R(+ + +)|a225>g|a244>g,N82>D(+ + -)|a250>g,K84>G(- - -)|a251>g,K84>G(- - -)|c259>g,L87>V(+ - +)|g261>c,L87>V(+ - +)|c276>t|c299>g,A100>G(- + -)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|c309>t|c314>t,A105>V(+ - +)| c86>a,T29>N(- + -)|t88>c,F30>L(+ - -)|g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|a112>g,S38>G(+ + -)| t135>g|g147>a|t163>c,S55>H(+ - -)|c164>a,S55>H(+ - -)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>a,S57>N(- - -)|a172>g,S58>G(+ + -)|t174>g,S58>G(+ + -)|a175>g,S59>G(+ + -)|g188>c,S63>T(+ - +)|t190>c,Y64>H(+ - -)| a206>g,D69>G(- - -)|a215>g,K72>R(+ + +)|a225>g|a244>g,N82>D(+ + -)|a250>g,K84>G(- - -)|a251>g,K84>G(- - -)|c259>g,L87>V(+ - +)|g261>c,L87>V(+ - +)|c276>t|c299>g,A100>G(- + -)|g301>t,V101>L(+ - +)|g303>a,V101>L(+ - +)|c309>t| c314>t,A105>V(+ - +)| 320 225 193 32 5 27 10 4 3 4 0 2 1 0 3 2 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 1 0 1 0 1 1 0 1 0 0 0 51 51 47 4 2 2 0 1 0 1 0 1 0 0 0 0 0 1 30 24 16 8 0 8 3 1 0 2 0 0 0 0 1 0 0 1 117 114 101 13 3 10 6 1 2 0 0 0 0 0 1 2 1 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaacctcaatacctttggc atgaactgggtccgccaggcgccagggaagggactggagtgggtctcacac gtcaatgggggtagtactcacata tactacgcaggctcagtgaggggccggttcaccatctccagagacgacgccgggaactcagtctatctgcaaatgaatagcctgagagccgaggacacgggtttatattattgt gtgagagacagtgactcgtccggctacgcgaggtacgactctatggacgtc VRDSDSSGYARYDSMDV IGHV3-21 IGHD6-19 IGHJ6 ggattcaacctcaatacctttggc atgaactgggtccgccaggcgccagggaagggactggagtgggtctcacac gtcaatgggggtagtactcacata tactacgcaggctcagtgaggggccggttcaccatctccagagacgacgccgggaactcagtctatctgcaaatgaatagcctgagagccgaggacacgggtttatattattgt gtgagagacagtgactcgtccggctacgcgaggtacgactctatggacgtc IGG2 31 213 20 11 6 14 14 17 5 0 12 14 JY8QFUQ01CNYY7 19200 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 810 86.18 187/217 nt 85.71 186/217 nt Homsap IGHJ3*01 F 169 82 41/50 nt Homsap IGHD3-16*01 F 3 8 7 12 8.7.12 [1.17.38.11] CARDDGGHMTLDVW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 62 of V-REGION: 1 nucleotides (from position 95 in the user submitted sequence), (cause frameshift) tgtctggattcacatttagaagctattccatgaattgggtccgccaggctccagggaaggggctggagtgggtctcagctgtcagtggtggtggggcgccacaaactacgcggagtccgtgaagggccggttcaccatctccagagacaattccagggggacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgtgcgagagatgacggcggacacatgactttagatgtctggggccaagggacaatggtcagcgtctctccggcctccaccaagggcccatcggtnttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactcanttccccgaaccggtgacggtgtgcgtggaactca NA IGG1 100 88 [285] c74>t|c75>g|c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a175>g,S59>G(+ + -)|t189>c|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|t196>a,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|a251>g,K84>R(+ + +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|c287>t,A96>V(+ - +)|g291>a|g297>a|g301>t,V101>L(+ - +)|c309>t|a317>g,K106>R(+ + +)| c74>t|c75>g| c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a175>g,S59>G(+ + -)|t189>c|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| t196>a,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|a251>g,K84>R(+ + +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c255>g,N85>G(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|c287>t,A96>V(+ - +)|g291>a|g297>a|g301>t,V101>L(+ - +)|c309>t| a317>g,K106>R(+ + +)| 320 225 194 31 10 21 8 4 5 3 0 2 1 1 1 4 2 0 78 5 3 2 2 0 0 0 1 0 0 0 0 0 0 1 0 0 36 24 21 3 1 2 0 0 0 0 0 2 0 0 0 0 1 0 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 23 17 6 1 5 3 0 0 2 0 0 0 0 1 0 0 0 117 114 97 17 6 11 4 3 3 1 0 0 1 1 0 3 1 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tgtct ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagct gtcagtggtggtggggcgccaca aactacgcggagtccgtgaagggccggttcaccatctccagagacaattccagggggacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgt gcgagagatgacggcggacacatgactttagatgtc ARDDGGHMTLDV IGHV3-23 IGHD3-16 IGHJ3 ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagct gtcagtggtggtggggcgccaca aactacgcggagtccgtgaagggccggttcaccatctccagagacaattccagggggacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgt gcgagagatgacggcggacacatgactttagatgtc IGG1 28 212 18 10 8 16 10 12 6 2 13 7 JY8QFUQ01CO06K 21756 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 911 90.5 200/221 nt 90.05 199/221 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 2 10 7 12 10.7.12 [1.17.38.11] CARHSRLVRYFEFW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'T' (1 nucleotides at position 54 to 54 in the user submitted sequence), (cause frameshift); tctctggtggctccatcaacagtggtagttatcactgggcctggatccgccagTcccccagggaaggggctggagtggattgggagcgtctcctatggtgggaacacctactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgtgcgagacattcgagactggtacggtactttgaattctggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacacgcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 86 89 [289, 290] g92>a,S31>N(- - -)|a103>g,S35>G(+ + -)|c111>t|t112>c,Y38>H(+ - -)|g119>c,G40>A(- + -)|t165>c|a166>g,I56>V(+ - +)|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a175>g,S59>G(+ + -)|g191>a,S64>N(- - -)|a215>t,K72>M(- + -)|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|c236>t,S79>F(- - -)|a240>c|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c261>g,F87>L(+ - -)|c271>g,L91>V(+ - +)|a291>g| g92>a,S31>N(- - -)|a103>g,S35>G(+ + -)|c111>t|t112>c,Y38>H(+ - -)| g119>c,G40>A(- + -)|t165>c| a166>g,I56>V(+ - +)|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a175>g,S59>G(+ + -)|g191>a,S64>N(- - -)| a215>t,K72>M(- + -)|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|c236>t,S79>F(- - -)|a240>c|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c261>g,F87>L(+ - -)|c271>g,L91>V(+ - +)|a291>g| 320 229 208 21 4 17 6 2 3 3 2 1 1 0 1 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 1 3 1 1 1 1 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 0 0 1 0 0 0 0 1 0 0 0 30 21 16 5 0 5 2 1 0 1 1 0 0 0 0 0 0 0 117 114 104 10 2 8 3 0 2 0 1 1 1 0 0 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcaacagtggtagttatcac tgggcctggatccgccagcccccagggaaggggctggagtggattgggagc gtctcctatggtgggaacacc tactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgt gcgagacattcgagactggtacggtactttgaattc ARHSRLVRYFEF IGHV4-39 IGHD5-18 IGHJ4 ggtggctccatcaacagtggtagttatcac tgggcctggatccgccagcccccagggaaggggctggagtggattgggagc gtctcctatggtgggaacacc tactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgt gcgagacattcgagactggtacggtactttgaattc IGA2 21 216 14 7 5 9 9 12 3 1 9 8 JY8QFUQ01CO7ZE 5992 productive Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F 872 89.77 193/215 nt NA Homsap IGHJ3*02 F 187 86 43/50 nt Homsap IGHD6-13*01 F 1 8 7 14 8.7.14 [1.17.38.11] CARPTYSSGRDAFDTW in-frame + tatatggtgggtccttcagtacttactactggacatggatccgccagcacccagagaagggactggagtggattggggaaatcaatcacagtggaagccccaactacagcccgtccctcaatagtcgagtcatcatatcgatagacacgtccaagaaccaggtctccctgaagctcttctctgtgaccgccgcggacacgggtgtgtactattgtgcgaggcccacgtatagtagcggccgggacgcctttgatacctggggccaagggacgacggtcaccgtctcatcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 100 100 [288] c75>a|g106>a,G36>T(+ - -)|g107>c,G36>T(+ - -)|g119>c,S40>T(+ - +)|c120>a,S40>T(+ - +)|c134>a,P45>H(+ - -)|g140>a,G47>E(- - -)|g147>a|t174>c|a193>c,T65>P(+ + -)|a203>g,N68>S(- - -)|g216>t,K72>N(+ - -)|c230>t,T77>I(- - -)|a237>g|g238>a,V80>I(+ - +)|t259>g,F87>V(+ - -)|g273>c|a274>t,S92>F(- - -)|g275>t,S92>F(- - -)|c299>g,A100>G(- + -)|t306>c|c309>t| c75>a| g106>a,G36>T(+ - -)|g107>c,G36>T(+ - -)| g119>c,S40>T(+ - +)|c120>a,S40>T(+ - +)|c134>a,P45>H(+ - -)|g140>a,G47>E(- - -)|g147>a| t174>c|a193>c,T65>P(+ + -)| a203>g,N68>S(- - -)|g216>t,K72>N(+ - -)|c230>t,T77>I(- - -)|a237>g|g238>a,V80>I(+ - +)|t259>g,F87>V(+ - -)|g273>c|a274>t,S92>F(- - -)|g275>t,S92>F(- - -)|c299>g,A100>G(- + -)|t306>c|c309>t| a318>g|g319>c|g320>c| 317 (320) 220 (223) 198 22 (25) 7 (10) 15 2 (3) 4 2 2 1 3 1 0 3 (5) 1 2 1 78 5 4 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 51 51 46 5 1 4 0 2 0 0 0 2 0 0 1 0 0 0 30 21 19 2 1 1 0 0 0 1 1 0 0 0 0 0 0 0 117 114 102 12 4 8 2 1 2 1 0 0 1 0 1 1 2 1 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 0 0 0 0 0 0 0 (2) 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tatat ggtgggtccttcagtacttactac tggacatggatccgccagcacccagagaagggactggagtggattggggaa atcaatcacagtggaagcccc aactacagcccgtccctcaatagtcgagtcatcatatcgatagacacgtccaagaaccaggtctccctgaagctcttctctgtgaccgccgcggacacgggtgtgtactattgt gcgaggcccacgtatagtagcggccgggacgcctttgatacc ARPTYSSGRDAFDT IGHV4-34 IGHD6-13 IGHJ3 ggtgggtccttcagtacttactac tggacatggatccgccagcacccagagaagggactggagtggattggggaa atcaatcacagtggaagcccc aactacagcccgtccctcaatagtcgagtcatcatatcgatagacacgtccaagaaccaggtctccctgaagctcttctctgtgaccgccgcggacacgggtgtgtactattgt gcgaggcccacgtatagtagcggccgggacgcctttgatacc IGA2 21 210 10 11 6 14 4 7 5 1 12 3 JY8QFUQ01COIZ4 13980 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 1008 96.31 209/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD1-26*01 F 1 8 8 13 8.8.13 [1.17.38.11] CARPGVGASSFIDFW in-frame + ttctggatacagctttaccgcctactggatcggctgggtgcgccagatgcccgggaaaggcctggagtgggtggcgatcatctatcctggtgactctgaaaccagatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcaccaccgcctacctgcattggaccagcctgaaggcctcggacaccgccatgtattactgtgcgagaccaggagtgggggcctcttctttcattgacttctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 100 100 [287] a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a157>g,M53>V(+ - -)|g161>c,G54>A(- + -)|t192>a,D64>E(+ - +)|g254>c,S85>T(+ - +)|g270>t,Q90>H(- + -)|g275>c,S92>T(+ - +)| a106>g,S36>A(- + -)|g107>c,S36>A(- + -)| a157>g,M53>V(+ - -)|g161>c,G54>A(- + -)| t192>a,D64>E(+ - +)| g254>c,S85>T(+ - +)|g270>t,Q90>H(- + -)|g275>c,S92>T(+ - +)| a320>c| 319 (320) 224 (225) 216 8 (9) 0 (1) 8 2 0 0 0 0 (1) 0 0 1 4 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 51 51 49 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 30 24 23 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 117 114 111 3 0 3 0 0 0 0 0 0 0 0 2 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ttct ggatacagctttaccgcctactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtgggtggcgatc atctatcctggtgactctgaaacc agatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcaccaccgcctacctgcattggaccagcctgaaggcctcggacaccgccatgtattactgt gcgagaccaggagtgggggcctcttctttcattgacttc ARPGVGASSFIDF IGHV5-51 IGHD1-26 IGHJ4 ggatacagctttaccgcctactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtgggtggcgatc atctatcctggtgactctgaaacc agatacagcccgtccttccaaggccaggtcaccatctcagccgacaagtccatcaccaccgcctacctgcattggaccagcctgaaggcctcggacaccgccatgtattactgt gcgagaccaggagtgggggcctcttctttcattgacttc IGA2 8 213 2 6 0 5 2 3 0 0 5 3 JY8QFUQ01COT7A 13255 productive Homsap IGHV3-7*02 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD5-12*01 F 1 8 8 9 8.8.9 [1.17.38.11] CARDQVTEDYW in-frame + ctctggattcctttttagaacctattggatgagttgggtctgccaggctccagggaaagggctggagtgggtggcccacataaaccaagatggacgtgaggcatactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgtgccagagatcaagtgactgaagactactggggccagggaaccctggtcagtgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [276] a85>c,T29>L(- - -)|c86>t,T29>L(- - -)|c87>t,T29>L(- - -)|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|c120>t|c127>t,R43>C(- - -)|a163>c,N55>H(- - -)|g171>c,K57>N(+ - -)|a187>c,S63>R(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)|c248>g,A83>G(- + -)|c255>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|g297>c|g301>a,V101>M(+ - -)|c309>t|g315>c| a85>c,T29>L(- - -)|c86>t,T29>L(- - -)|c87>t,T29>L(- - -)|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)| c120>t|c127>t,R43>C(- - -)|a163>c,N55>H(- - -)| g171>c,K57>N(+ - -)|a187>c,S63>R(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)| c248>g,A83>G(- + -)|c255>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|g297>c|g301>a,V101>M(+ - -)|c309>t| g315>c| 318 223 202 21 6 15 1 2 7 0 4 0 0 1 4 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 0 2 0 1 0 0 1 1 0 0 0 51 51 48 3 1 2 0 0 2 0 1 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 0 0 2 0 0 0 1 0 0 0 117 114 106 8 4 4 0 2 3 0 0 0 0 0 1 2 0 0 6 6 5 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcctttttagaacctattgg atgagttgggtctgccaggctccagggaaagggctggagtgggtggcccac ataaaccaagatggacgtgaggca tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac ARDQVTEDY IGHV3-7 IGHD5-12 IGHJ4 ggattcctttttagaacctattgg atgagttgggtctgccaggctccagggaaagggctggagtgggtggcccac ataaaccaagatggacgtgaggca tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac IGG1 20 213 10 10 9 14 1 6 5 0 6 9 JY8QFUQ01COULV 16546 productive Homsap IGHV4-61*02 F 888 89.55 197/220 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-19*01 F 1 10 7 13 10.7.13 [1.17.38.11] CARQGDSSGPWNDYW in-frame + ctccggtggctccatcagtactggttattactactggagctggatccggcagtccgccgggaagggactggaatggattgggcgcatgtctgccagaggggacagcaactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgtgcgagacaaggggatagtagtggaccctggaatgactactggggccagggaatcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG1 100 100 [291] t78>c|c93>t|g101>c,S34>T(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c133>t,P45>S(+ - -)|g153>a|t165>c|c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>g,T65>S(+ - +)|a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| t78>c| c93>t|g101>c,S34>T(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)| c133>t,P45>S(+ - -)|g153>a|t165>c| c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>g,T65>S(+ - +)| a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g319>c| 318 (320) 226 (228) 203 (204) 23 (24) 5 (6) 18 3 4 3 2 3 0 2 1 2 (3) 3 0 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 30 26 4 1 3 0 1 1 0 0 0 1 0 1 0 0 0 51 51 48 3 2 1 0 1 1 1 0 0 0 0 0 0 0 0 30 21 14 7 0 7 2 1 0 0 1 0 0 1 0 2 0 0 117 114 106 8 1 7 1 1 1 0 2 0 1 0 1 1 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctcc ggtggctccatcagtactggttattactac tggagctggatccggcagtccgccgggaagggactggaatggattgggcgc atgtctgccagaggggacagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac ARQGDSSGPWNDY IGHV4-61 IGHD6-19 IGHJ4 ggtggctccatcagtactggttattactac tggagctggatccggcagtccgccgggaagggactggaatggattgggcgc atgtctgccagaggggacagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac IGG1 22 216 11 11 7 12 4 10 3 1 8 10 JY8QFUQ01CP6A0 16373 productive (see comment) Homsap IGHV3-73*01 F, or Homsap IGHV3-73*02 F 1007 94.64 212/224 nt 93.75 210/224 nt Homsap IGHJ6*01 F (see comment) 180 76.19 48/63 nt Homsap IGHD4-11*01 ORF 3 8 10 18 8.10.18 [1.17.38.11] CVRHEGTPVSDVGGYGMDVW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*01 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 59 to 59 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 52 of V-REGION: 'G' (1 nucleotides at position 70 to 70 in the user submitted sequence), (cause frameshift); cctctggattcaccttcagtggctctgctatgcactgggtccgccaggtttccgggaaAagggctggagGtgggttggccgtattagaagcaaagcttacaattccgcgacagcatatgctgcgtcggtgaaaggcaggttcaccatctccagagatgattcaaagaacacggcgtatttggaaatgaacagtttgaagagggaggacacggccgtgtattactgtgtgagacatgaagggactccagtaagtgatgtaggtggatacggcatggacgtctggggtcaagggtccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 100 100 [311] g81>a|c134>t,A45>V(+ - +)|a181>t,N61>Y(- - -)|g185>a,S62>N(- - -)|a188>c,Y63>S(+ - -)|c265>t|c268>g,Q90>E(+ + -)|c279>t|c280>t|a285>g|c287>g,T96>R(- - -)|c288>g,T96>R(- - -)| g81>a| c134>t,A45>V(+ - +)| a181>t,N61>Y(- - -)|g185>a,S62>N(- - -)|a188>c,Y63>S(+ - -)| c265>t|c268>g,Q90>E(+ + -)|c279>t|c280>t|a285>g|c287>g,T96>R(- - -)|c288>g,T96>R(- - -)| a313>g,T105>V(- - -)|c314>t,T105>V(- - -)|t315>g,T105>V(- - -)| 320 232 217 15 5 10 2 2 5 0 1 0 1 0 0 3 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 30 27 3 0 3 0 1 0 0 1 0 1 0 0 0 0 0 117 114 107 7 4 3 1 0 3 0 0 0 0 0 0 3 0 0 8 8 5 3 0 3 1 0 1 0 0 0 0 0 0 0 0 1 aa,58-59(FR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,114-115(CDR1)|tt,135-136(FR2)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|ggct,106-109(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|agca,195-198(CDR2)|ggca,220-223(FR3)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,59-62(FR1)|agcc,72-75(FR1)|tgct,111-114(CDR1)|agct,177-180(CDR2)|tgct,201-204(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| cctct ggattcaccttcagtggctctgct atgcactgggtccgccaggtttccgggaaagggctggagtgggttggccgt attagaagcaaagcttacaattccgcgaca gcatatgctgcgtcggtgaaaggcaggttcaccatctccagagatgattcaaagaacacggcgtatttggaaatgaacagtttgaagagggaggacacggccgtgtattactgt gtgagacatgaagggactccagtaagtgatgtaggtggatacggcatggacgtc VRHEGTPVSDVGGYGMDV IGHV3-73 IGHD4-11 IGHJ6 ggattcaccttcagtggctctgct atgcactgggtccgccaggtttccgggaaagggctggagtgggttggccgt attagaagcaaagcttacaattccgcgaca gcatatgctgcgtcggtgaaaggcaggttcaccatctccagagatgattcaaagaacacggcgtatttggaaatgaacagtttgaagagggaggacacggccgtgtattactgt gtgagacatgaagggactccagtaagtgatgtaggtggatacggcatggacgtc IGA2 12 219 7 5 6 9 1 3 4 1 4 3 JY8QFUQ01CPEX7 21686 productive (see comment) Homsap IGHV4-4*02 F, or Homsap IGHV4-4*03 F or Homsap IGHV4-4*04 F or Homsap IGHV4-4*05 F or Homsap IGHV4-55*03 P 815 86.24 188/218 nt 85.32 186/218 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-22*01 F 2 9 7 15 9.7.15 [1.17.38.11] CTSREHAFSGQFTGDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'TCA' (3 nucleotides at position 25 to 27 in the user submitted sequence), (do not cause frameshift);in CDR2-IMGT at codon 58 of V-REGION: 'T' (1 nucleotides at position 94 to 94 in the user submitted sequence), (cause frameshift); tgtctggtgtctccgtcaccagcgTCAgtcactggtggacctgggtccgccagcccccagggaagggactggagtggattggagaaatctattTattatggcatcaccaatttcaacccgtccctcaagagtcgaatcagcatgtcagtggacgagtccaagaaccagttctccctgagactgacttctgttaccgccgcggacacggccgtttattattgtacgagtcgcgaacatgcttttagtggtcaattcacgggtgactactggggccagggaaccctgatgaccgtcacctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [299] c75>g|g83>t,G28>V(- - -)|a88>g,I30>V(+ - +)|g92>c,S31>T(+ - +)|t105>c|a106>g,S36>G(+ + -)|a109>c,N37>H(- - -)|g119>c,S40>T(+ - +)|t120>c,S40>T(+ - +)|g147>a|g162>a|c172>t,H58>Y(+ - -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|g189>c|g191>t,S64>I(- - -)|c198>t|a200>t,Y67>F(- + -)|g226>a,V76>I(+ - +)|c230>g,T77>S(+ - +)|a234>g,I78>M(+ + -)|a240>g|a244>g,K82>E(+ - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g282>t|g303>t|c309>t|g313>a,A105>T(- - -)| c75>g| g83>t,G28>V(- - -)|a88>g,I30>V(+ - +)|g92>c,S31>T(+ - +)|t105>c|a106>g,S36>G(+ + -)|a109>c,N37>H(- - -)| g119>c,S40>T(+ - +)|t120>c,S40>T(+ - +)|g147>a|g162>a| c172>t,H58>Y(+ - -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|g189>c|g191>t,S64>I(- - -)| c198>t|a200>t,Y67>F(- + -)|g226>a,V76>I(+ - +)|c230>g,T77>S(+ - +)|a234>g,I78>M(+ + -)|a240>g|a244>g,K82>E(+ - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g282>t|g303>t|c309>t| g313>a,A105>T(- - -)|a318>t,R106>S(- - -)|g319>c|a320>g| 317 (320) 223 (226) 192 31 (34) 10 (12) 21 (22) 6 (7) 6 4 2 1 0 2 (3) 0 4 (5) 2 4 0 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 27 21 6 1 5 2 0 0 1 1 0 0 0 1 0 1 0 51 51 47 4 2 2 0 2 0 1 0 0 0 0 1 0 0 0 30 21 16 5 1 4 0 1 1 0 0 0 1 0 1 0 1 0 117 114 100 14 5 9 4 2 3 0 0 0 1 0 1 1 2 0 5 (8) 5 4 1 (4) 0 (2) 1 (2) 0 (1) 1 0 0 0 0 0 (1) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tgtct ggtgtctccgtcaccagcggtcactgg tggacctgggtccgccagcccccagggaagggactggagtggattggagaa atctattattatggcatcacc aatttcaacccgtccctcaagagtcgaatcagcatgtcagtggacgagtccaagaaccagttctccctgagactgacttctgttaccgccgcggacacggccgtttattattgt acgagtcgcgaacatgcttttagtggtcaattcacgggtgactac TSREHAFSGQFTGDY IGHV4-4 IGHD3-22 IGHJ4 ggtgtctccgtcaccagcggtcactgg tggacctgggtccgccagcccccagggaagggactggagtggattggagaa atctattattatggcatcacc aatttcaacccgtccctcaagagtcgaatcagcatgtcagtggacgagtccaagaaccagttctccctgagactgacttctgttaccgccgcggacacggccgtttattattgt acgagtcgcgaacatgcttttagtggtcaattcacgggtgactac IGG2 29 213 17 12 9 18 8 11 7 2 11 9 JY8QFUQ01CPKFW 15434 productive Homsap IGHV3-72*01 F 884 89.14 197/221 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD2-15*01 F 3 8 10 9 8.10.9 [0.17.38.11] CAVAAPPYDGW in-frame - ctggattcaccttaagtgacttctacatggactggctccgccaggctccagggaaggggctggagtgggttggtcgcagtagaaacaaagctaatggttacagtacacaattcgccgcatctgtgatgggcagattcaccatctcaagagatgactcaaagaatttactatatctacaaatgagcagcctgaaaaccgaggacacggccatttattactgtgctgtcgcagcccccccttatgacggctggggccagggaaccctggtcatcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [279] c90>a,F30>L(+ - -)|c109>t,H37>F(- - -)|a110>t,H37>F(- - -)|g124>c,V42>L(+ - +)|c162>t|t165>c|c167>g,T56>S(+ - +)|c183>t|a184>g,S62>G(+ + -)|c191>g,T64>S(+ - +)|c192>t,T64>S(+ - +)|g196>c,E66>Q(+ + -)|a200>t,Y67>F(- + -)|g207>a|a215>t,K72>M(- + -)|a216>g,K72>M(- + -)|t246>c|c255>t|c257>t,S86>L(- - -)|g261>a|g267>a|a275>g,N92>S(- - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| c90>a,F30>L(+ - -)|c109>t,H37>F(- - -)|a110>t,H37>F(- - -)| g124>c,V42>L(+ - +)|c162>t|t165>c| c167>g,T56>S(+ - +)|c183>t|a184>g,S62>G(+ + -)|c191>g,T64>S(+ - +)|c192>t,T64>S(+ - +)| g196>c,E66>Q(+ + -)|a200>t,Y67>F(- + -)|g207>a|a215>t,K72>M(- + -)|a216>g,K72>M(- + -)|t246>c|c255>t|c257>t,S86>L(- - -)|g261>a|g267>a|a275>g,N92>S(- - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| a316>g,R106>V(- - -)|g317>t,R106>V(- - -)|a318>c,R106>V(- - -)|a320>c| 315 (320) 224 (229) 200 (201) 24 (28) 8 (9) 16 (19) 3 (4) 4 6 2 0 (2) 1 3 0 2 2 1 (2) 0 78 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 1 0 0 1 1 0 0 0 0 0 51 51 48 3 2 1 0 0 1 1 0 0 0 0 1 0 0 0 30 30 25 5 1 4 1 0 2 0 0 0 0 0 0 2 0 0 117 114 101 13 5 8 2 4 2 1 0 0 2 0 1 0 1 0 3 (8) 3 3 (4) 0 (4) 0 (1) 0 (3) 0 (1) 0 0 0 0 (2) 0 0 0 0 0 0 (1) 0 ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,165-168(FR2-CDR2)|agct,177-180(CDR2)|aact,253-256(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tgct,312-315(FR3)| ct ggattcaccttaagtgacttctac atggactggctccgccaggctccagggaaggggctggagtgggttggtcgc agtagaaacaaagctaatggttacagtaca caattcgccgcatctgtgatgggcagattcaccatctcaagagatgactcaaagaatttactatatctacaaatgagcagcctgaaaaccgaggacacggccatttattactgt gctgtcgcagcccccccttatgacggc AVAAPPYDG IGHV3-72 IGHD2-15 IGHJ4 ggattcaccttaagtgacttctac atggactggctccgccaggctccagggaaggggctggagtgggttggtcgc agtagaaacaaagctaatggttacagtaca caattcgccgcatctgtgatgggcagattcaccatctcaagagatgactcaaagaatttactatatctacaaatgagcagcctgaaaaccgaggacacggccatttattactgt gctgtcgcagcccccccttatgacggc IGA2 24 219 15 9 10 16 5 8 7 1 9 7 JY8QFUQ01CQ2DI 12704 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 914 91.28 199/218 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD1-26*01 F 1 8 8 9 8.8.9 [1.17.38.11] CTREVGATDYW in-frame - cctctcgattcacctttagcagctatgtcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcacgtattcatagtgatgggactaccacatactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaggaacacgttgtatctgcaattgaacagtctgagagccgaggacacggctgtgtattattgtacaagagaggtgggagctaccgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccca NA IGA2 79 95 [276, 275] g79>c,G27>R(- - -)|c90>t|t105>c|c111>t|t112>g,W38>V(+ - -)|g113>t,W38>V(+ - -)|g114>c,W38>V(+ - -)|c118>a,H40>N(- - -)|a132>g|t152>a,V51>E(- + -)|a169>c,N57>H(- - -)|g188>c,S63>T(+ - +)|g191>c,S64>T(+ - +)|a196>t,S66>Y(+ - -)|g197>a,S66>Y(+ - -)|a251>g,K84>R(+ + +)|c259>t|a271>t,M91>L(+ + -)|c309>t|g313>a,A105>T(- - -)| g79>c,G27>R(- - -)|c90>t|t105>c|c111>t|t112>g,W38>V(+ - -)|g113>t,W38>V(+ - -)|g114>c,W38>V(+ - -)| c118>a,H40>N(- - -)|a132>g|t152>a,V51>E(- + -)| a169>c,N57>H(- - -)|g188>c,S63>T(+ - +)|g191>c,S64>T(+ - +)| a196>t,S66>Y(+ - -)|g197>a,S66>Y(+ - -)|a251>g,K84>R(+ + +)|c259>t|a271>t,M91>L(+ + -)|c309>t| g313>a,A105>T(- - -)| 320 226 206 20 6 14 2 2 4 1 1 1 2 1 4 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 3 4 0 0 2 1 0 0 0 0 2 0 1 1 51 51 48 3 1 2 1 0 0 0 0 1 0 1 0 0 0 0 30 24 21 3 0 3 0 0 0 0 1 0 0 0 2 0 0 0 117 114 108 6 2 4 1 1 2 0 0 0 2 0 0 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct cgattcacctttagcagctatgtc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcacgt attcatagtgatgggactaccaca tactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaggaacacgttgtatctgcaattgaacagtctgagagccgaggacacggctgtgtattattgt acaagagaggtgggagctaccgactac TREVGATDY IGHV3-74 IGHD1-26 IGHJ4 cgattcacctttagcagctatgtc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcacgt attcatagtgatgggactaccaca tactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaggaacacgttgtatctgcaattgaacagtctgagagccgaggacacggctgtgtattattgt acaagagaggtgggagctaccgactac IGA2 19 213 8 11 5 11 3 8 3 3 6 7 JY8QFUQ01CQFLG 7063 productive Homsap IGHV4-31*02 F, or Homsap IGHV4-31*03 F or Homsap IGHV4-31*04 F 920 90.95 201/221 nt NA Homsap IGHJ1*01 F 242 96.15 50/52 nt Homsap IGHD2-21*02 F 2 10 7 18 10.7.18 [1.17.38.11] CARSAYCGADCYSADYLQHW in-frame + tctctggtggctccgtcagcagtaggggttactactggaactggatccgccagttcccagggaagggcctggagtggattgggaacatcttttacagtgggggcacctacgacaacccgtccctcaggagtcgaatttctatatcattagacacgtctaagaaccaattctccctgaagttgacctctatgaccgccgcggacacggccgtgtattactgtgcgagatcggcatactgtggtgctgactgctactcagctgattacctccagcactggggccagggcaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgntgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 100 89 [306] a88>g,I30>V(+ - +)|g103>a,G35>R(- - -)|t105>g,G35>R(- - -)|g119>a,S40>N(- - -)|c133>t,H45>F(- - -)|a134>t,H45>F(- - -)|t163>a,Y55>N(- - -)|a170>t,Y57>F(- + -)|a190>g,S64>G(+ + -)|t199>g,Y67>D(- - -)|a215>g,K72>R(+ + +)|g226>a,V76>I(+ - +)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|g238>t,V80>L(+ - +)|g258>a|c271>t|g275>c,S92>T(+ - +)|g280>a,V94>M(+ - -)|t285>c| a88>g,I30>V(+ - +)|g103>a,G35>R(- - -)|t105>g,G35>R(- - -)| g119>a,S40>N(- - -)|c133>t,H45>F(- - -)|a134>t,H45>F(- - -)|t163>a,Y55>N(- - -)| a170>t,Y57>F(- + -)|a190>g,S64>G(+ + -)| t199>g,Y67>D(- - -)|a215>g,K72>R(+ + +)|g226>a,V76>I(+ - +)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|g238>t,V80>L(+ - +)|g258>a|c271>t|g275>c,S92>T(+ - +)|g280>a,V94>M(+ - -)|t285>c| g319>t|a320>c| 318 (320) 227 (229) 207 20 (22) 3 (5) 17 3 5 3 1 0 (1) 0 3 1 1 0 1 (2) 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 1 1 0 0 0 0 0 0 0 0 0 1 51 51 47 4 0 4 0 1 1 0 0 0 1 1 0 0 0 0 30 21 19 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 117 114 103 11 3 8 1 3 2 1 0 0 1 0 1 0 1 1 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,228-229(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,69-70(FR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,227-228(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|agca,131-134(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agtt,225-228(FR3)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,69-72(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|tacc,228-231(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tgcc,285-288(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcagcagtaggggttactac tggaactggatccgccagttcccagggaagggcctggagtggattgggaac atcttttacagtgggggcacc tacgacaacccgtccctcaggagtcgaatttctatatcattagacacgtctaagaaccaattctccctgaagttgacctctatgaccgccgcggacacggccgtgtattactgt gcgagatcggcatactgtggtgctgactgctactcagctgattacctccagcac ARSAYCGADCYSADYLQH IGHV4-31 IGHD2-21 IGHJ1 ggtggctccgtcagcagtaggggttactac tggaactggatccgccagttcccagggaagggcctggagtggattgggaac atcttttacagtgggggcacc tacgacaacccgtccctcaggagtcgaatttctatatcattagacacgtctaagaaccaattctccctgaagttgacctctatgaccgccgcggacacggccgtgtattactgt gcgagatcggcatactgtggtgctgactgctactcagctgattacctccagcac IGA2 20 216 12 8 8 10 4 10 3 0 12 5 JY8QFUQ01CQHUH 13871 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 882 89.86 195/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [1.17.38.11] CTRDIGGRYSYW in-frame + ctccggattcacctttactaattattggatgcactgggtccgccaagctccaggggagggactggtgtgggtctcacggactaatgaagatgggagtatcacaaactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctcagagtcgaggacacggctatatattactgtacaagagacatcggtgggcgctactcctactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [278] t78>c|c90>t|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|c111>t|a142>g,K48>E(+ - -)|g147>a|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|g282>c|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|g313>a,A105>T(- - -)| t78>c| c90>t|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|c111>t| a142>g,K48>E(+ - -)|g147>a|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|g282>c|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g313>a,A105>T(- - -)| 320 225 202 23 6 17 3 8 4 2 0 0 0 1 2 1 1 1 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 1 3 0 0 0 0 0 1 0 0 0 51 51 48 3 2 1 1 1 0 0 0 0 0 0 0 0 0 1 30 24 19 5 0 5 1 1 0 1 0 0 0 1 0 0 1 0 117 114 106 8 1 7 1 4 1 0 0 0 0 0 1 1 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcacctttactaattattgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctcagagtcgaggacacggctatatattactgt acaagagacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttactaattattgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctcagagtcgaggacacggctatatattactgt acaagagacatcggtgggcgctactcctac IGA1 21 213 15 6 11 15 4 6 3 2 8 8 JY8QFUQ01CQKI9 20701 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 945 93.09 202/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-25*01 F 2 8 8 16 8.8.16 [1.17.38.11] CAKDLLGIAPAGHSLDHW in-frame + ctctggattcacctttggcaactatgccatgacctgggtccgccaggctccagggaaggggctggagtgggtctcagctattagtggtagaggtgaccacacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccaggaacacactgtttctgcaaatgaatagcctgggagtcgaggacacgaccgtatattactgtgcgaaggatcttctagggattgcgccggcgggccattcccttgaccactggggccagggaaccctggtcgccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcaccccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 79 95 [296, 297] a103>g,S35>G(+ + -)|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|t177>a,S59>R(- - -)|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|a251>g,K84>R(+ + +)|g258>a|a263>t,Y88>F(- + -)|c276>t|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g298>a,A100>T(- - -)|a318>g| a103>g,S35>G(+ + -)|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)| t177>a,S59>R(- - -)|g188>a,G63>D(- - -)|t189>c,G63>D(- - -)|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)| a251>g,K84>R(+ + +)|g258>a|a263>t,Y88>F(- + -)|c276>t|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|g298>a,A100>T(- - -)| a318>g| 320 225 209 16 3 13 4 5 2 1 1 0 1 1 1 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 19 5 0 5 0 2 0 1 1 0 0 1 0 0 0 0 117 114 107 7 2 5 2 2 2 0 0 0 1 0 0 0 0 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttggcaactatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcagct attagtggtagaggtgaccacaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaggaacacactgtttctgcaaatgaatagcctgggagtcgaggacacgaccgtatattactgt gcgaaggatcttctagggattgcgccggcgggccattcccttgaccac AKDLLGIAPAGHSLDH IGHV3-23 IGHD6-25 IGHJ4 ggattcacctttggcaactatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcagct attagtggtagaggtgaccacaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaggaacacactgtttctgcaaatgaatagcctgggagtcgaggacacgaccgtatattactgt gcgaaggatcttctagggattgcgccggcgggccattcccttgaccac IGA1 15 213 11 4 7 8 4 7 2 0 6 7 JY8QFUQ01CQOIV 3832 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 738 82.49 179/217 nt NA Homsap IGHJ3*01 F 187 86 43/50 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [1.17.38.11] CVKDKAGFTLHAFDVW in-frame + Low V-REGION identity (82,49% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctggattcgactttgccagccacgccatggcttgggtccgccggactccaaggaagggcctggagtgggtctcaggcataagtagtagtggtggaaccacgtattacgcagacttcgtgaagggccgcgccactgtctccagagacaattccgagaacacagtgtctctggaactccacagcctgagagccgatgacacggccatatattattgtgtgaaggacaaagcgggcttcaccctccatgcttttgatgtctggggccgagggacggtggtcactgtttcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [290] a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c120>t,S40>A(- + -)|a131>g,Q44>R(+ - -)|g133>a,A45>T(- - -)|g139>a,G47>R(- - -)|g147>c|c164>g,A55>G(- + -)|t165>c,A55>G(- + -)|t168>a|g172>a,G58>S(+ + -)|t189>a|g191>c,S64>T(+ - +)|a195>g|c198>t|c209>t,S70>F(- - -)|g225>c|t226>g,F76>A(+ - -)|t227>c,F76>A(+ - -)|c231>t|a232>g,I78>V(+ - +)|a250>g,K84>E(+ - -)|g258>a|c259>g,L87>V(+ - +)|a263>c,Y88>S(+ - -)|c268>g,Q90>E(+ + -)|a271>c,M91>L(+ + -)|g273>c,M91>L(+ + -)|a274>c,N92>H(- - -)|g291>t,E97>D(+ - +)|g301>a,V101>I(+ - +)|c309>t|c314>t,A105>V(+ - +)|a318>g| a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c120>t,S40>A(- + -)|a131>g,Q44>R(+ - -)|g133>a,A45>T(- - -)|g139>a,G47>R(- - -)|g147>c|c164>g,A55>G(- + -)|t165>c,A55>G(- + -)| t168>a|g172>a,G58>S(+ + -)|t189>a|g191>c,S64>T(+ - +)|a195>g| c198>t|c209>t,S70>F(- - -)|g225>c|t226>g,F76>A(+ - -)|t227>c,F76>A(+ - -)|c231>t|a232>g,I78>V(+ - +)|a250>g,K84>E(+ - -)|g258>a|c259>g,L87>V(+ - +)|a263>c,Y88>S(+ - -)|c268>g,Q90>E(+ + -)|a271>c,M91>L(+ + -)|g273>c,M91>L(+ + -)|a274>c,N92>H(- - -)|g291>t,E97>D(+ - +)|g301>a,V101>I(+ - +)|c309>t| c314>t,A105>V(+ - +)|a318>g| 320 225 185 40 10 30 8 5 6 4 3 1 0 2 6 3 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 2 0 0 2 0 1 0 0 1 0 0 0 51 51 42 9 1 8 2 2 1 1 0 0 0 0 2 1 0 0 30 24 19 5 3 2 1 1 0 0 0 0 0 2 1 0 0 0 117 114 96 18 5 13 2 2 4 1 3 0 0 0 2 2 1 1 8 8 6 2 1 1 1 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcgactttgccagccacgcc atggcttgggtccgccggactccaaggaagggcctggagtgggtctcaggc ataagtagtagtggtggaaccacg tattacgcagacttcgtgaagggccgcgccactgtctccagagacaattccgagaacacagtgtctctggaactccacagcctgagagccgatgacacggccatatattattgt gtgaaggacaaagcgggcttcaccctccatgcttttgatgtc VKDKAGFTLHAFDV IGHV3-23 IGHD5-24 IGHJ3 ggattcgactttgccagccacgcc atggcttgggtccgccggactccaaggaagggcctggagtgggtctcaggc ataagtagtagtggtggaaccacg tattacgcagacttcgtgaagggccgcgccactgtctccagagacaattccgagaacacagtgtctctggaactccacagcctgagagccgatgacacggccatatattattgt gtgaaggacaaagcgggcttcaccctccatgcttttgatgtc IGA1 38 213 21 17 10 21 11 17 6 3 21 8 JY8QFUQ01CQRVK 2151 productive (see comment) Homsap IGHV4-4*02 F, or Homsap IGHV4-4*03 F or Homsap IGHV4-4*04 F or Homsap IGHV4-4*05 F 976 94.91 205/216 nt 93.98 203/216 nt Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD2-21*02 F 3 9 7 13 9.7.13 [1.16.38.11] CARGEMTAEYYFDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 37 of V-REGION: 'C' (1 nucleotides at position 26 to 26 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 54 of V-REGION: 1 nucleotides (from position 79 in the user submitted sequence), (cause frameshift) ctctggtggctccatcagcagtgttCaactggtggagttgggtccgccagcccccagggaaggggctggagtggattgggagatctctcacagtgggaacaccaactacagcccgtccctcaagggtcgagtcaccatatcaataaacaagtccaagaaccaattctccctgaagctgagctctgtgaccgccgcggacacggccgtgtattactgtgcgagaggggaaatgactgccgagtactactttgattactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [287, 286] a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|a165>g|a170>c,Y57>S(+ - -)|t174>c|g191>a,S64>N(- - -)|a203>g,N68>S(- - -)|a220>g,S74>G(+ + -)|g238>a,V80>I(+ - +)|g241>a,D81>N(+ + -)|g258>a| a106>g,S36>V(- - -)|g107>t,S36>V(- - -)| a165>g| a170>c,Y57>S(+ - -)|t174>c|g191>a,S64>N(- - -)| a203>g,N68>S(- - -)|a220>g,S74>G(+ + -)|g238>a,V80>I(+ - +)|g241>a,D81>N(+ + -)|g258>a| a320>g| 319 (320) 223 (224) 212 11 (12) 3 (4) 8 4 (5) 4 0 1 1 0 0 0 0 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 27 25 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 51 50 49 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 30 21 18 3 1 2 0 1 0 1 1 0 0 0 0 0 0 0 117 114 109 5 1 4 2 3 0 0 0 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtggctccatcagcagtgttaactgg tggagttgggtccgccagcccccagggaaggggctggagtggattgggag atctctcacagtgggaacacc aactacagcccgtccctcaagggtcgagtcaccatatcaataaacaagtccaagaaccaattctccctgaagctgagctctgtgaccgccgcggacacggccgtgtattactgt gcgagaggggaaatgactgccgagtactactttgattac ARGEMTAEYYFDY IGHV4-4 IGHD2-21 IGHJ4 ggtggctccatcagcagtgttaactgg tggagttgggtccgccagcccccagggaaggggctggagtggattgggag atctctcacagtgggaacacc aactacagcccgtccctcaagggtcgagtcaccatatcaataaacaagtccaagaaccaattctccctgaagctgagctctgtgaccgccgcggacacggccgtgtattactgt gcgagaggggaaatgactgccgagtactactttgattac IGA2 11 212 9 2 4 5 5 6 2 1 4 4 JY8QFUQ01CQSBL 9336 productive (see comment) Homsap IGHV4-61*02 F 875 90.57 192/212 nt 89.62 190/212 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-19*01 F 1 8 7 13 8.7.13 [0.17.38.11] CARQGDSSGPWNDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'CTGGTT' (6 nucleotides at position 19 to 24 in the user submitted sequence), (do not cause frameshift);in CDR2-IMGT at codon 64 of V-REGION: 'A' (1 nucleotides at position 101 to 101 in the user submitted sequence), (cause frameshift); ccggtggctccatcagtaCTGGTTattactactggagctggatccggcagtccgccgggaagggactggaatggattgggcgcatgtctgccagagggggAcagcaactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgtgcgagacaaggggatagtagtggaccctggaatgactactggggccagggaatcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [290] t78>c|g103>a,G35>S(+ + -)|g107>a,S36>N(- - -)|c133>t,P45>S(+ - -)|g153>a|t165>c|c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>G(+ + -)|c194>g,T65>S(+ - +)|a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| t78>c| g103>a,G35>S(+ + -)|g107>a,S36>N(- - -)| c133>t,P45>S(+ - -)|g153>a|t165>c| c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>G(+ + -)|c194>g,T65>S(+ - +)| a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g319>c| 318 (320) 218 (220) 198 (199) 20 (21) 4 (5) 16 3 4 2 2 3 0 1 1 1 (2) 3 0 0 78 2 1 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 2 0 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 1 0 0 0 0 0 0 0 0 30 21 15 6 0 6 2 0 0 0 1 0 0 1 0 2 0 0 117 114 106 8 1 7 1 1 1 0 2 0 1 0 1 1 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| cc ggtggctccatcagtaattactac tggagctggatccggcagtccgccgggaagggactggaatggattgggcgc atgtctgccagagggggcagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac ARQGDSSGPWNDY IGHV4-61 IGHD6-19 IGHJ4 ggtggctccatcagtaattactac tggagctggatccggcagtccgccgggaagggactggaatggattgggcgc atgtctgccagagggggcagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac IGG1 19 210 10 9 6 10 4 9 3 0 8 8 JY8QFUQ01CQWKF 3933 productive (see comment) Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 836 87.91 189/215 nt 87.44 188/215 nt Homsap IGHJ6*01 F (see comment) 171 74.6 47/63 nt Homsap IGHD2-2*01 F 1 7 8 13 7.8.13 [1.17.38.11] CVSDRELHYQHLDVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) in CDR1-IMGT, from codon 30 of V-REGION: 1 nucleotides (from position 14 in the user submitted sequence), (cause frameshift) tctggattcaactcaatagttttggcatgcactgggtccgccaggctccgggcaagggactggagtgggtggcaaacatatggtatgatggaggtagtcaacactatgcagacctcgtgaagggccgattcaccatctctagagacaattccaagaacatcttgttcctgcaaatgagcgacctgagagccgaggacacggctgtttattattgtgtgagcgatagagaactccattaccagcatttggacgtctggggtcaagggaccacggtcatcgtctcatcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [286] c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|c108>t|a110>t,Y37>F(- + -)|a138>g|g147>a|g163>a,V55>N(- - -)|t164>a,V55>N(- - -)|t165>c,V55>N(- - -)|a187>g,S63>G(+ + -)|a191>g,N64>S(- - -)|a193>c,K65>Q(+ - -)|t196>c,Y66>H(+ - -)|t208>c,S70>L(- - -)|c209>t,S70>L(- - -)|c237>t|c257>t,T86>I(- - -)|g258>c,T86>I(- - -)|c259>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|a275>g,N92>S(- - -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g303>t|c309>t|c314>t,A105>V(+ - +)|a318>c,R106>S(- - -)| c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|c108>t|a110>t,Y37>F(- + -)| a138>g|g147>a|g163>a,V55>N(- - -)|t164>a,V55>N(- - -)|t165>c,V55>N(- - -)| a187>g,S63>G(+ + -)|a191>g,N64>S(- - -)|a193>c,K65>Q(+ - -)| t196>c,Y66>H(+ - -)|t208>c,S70>L(- - -)|c209>t,S70>L(- - -)|c237>t|c257>t,T86>I(- - -)|g258>c,T86>I(- - -)|c259>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|a275>g,N92>S(- - -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g303>t|c309>t| c314>t,A105>V(+ - +)|a318>c,R106>S(- - -)| 320 223 195 28 7 21 5 4 7 4 2 1 2 1 1 0 1 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 19 4 1 3 0 1 1 0 0 1 1 0 0 0 0 0 51 51 46 5 2 3 1 2 0 1 0 0 0 1 0 0 0 0 30 24 21 3 0 3 2 0 0 0 1 0 0 0 0 0 0 0 117 114 100 14 4 10 2 1 5 3 0 0 1 0 1 0 1 0 8 8 6 2 0 2 0 0 1 0 1 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcaactcaatagttttggc atgcactgggtccgccaggctccgggcaagggactggagtgggtggcaaac atatggtatgatggaggtagtcaa cactatgcagacctcgtgaagggccgattcaccatctctagagacaattccaagaacatcttgttcctgcaaatgagcgacctgagagccgaggacacggctgtttattattgt gtgagcgatagagaactccattaccagcatttggacgtc VSDRELHYQHLDV IGHV3-33 IGHD2-2 IGHJ6 ggattcaactcaatagttttggc atgcactgggtccgccaggctccgggcaagggactggagtgggtggcaaac atatggtatgatggaggtagtcaa cactatgcagacctcgtgaagggccgattcaccatctctagagacaattccaagaacatcttgttcctgcaaatgagcgacctgagagccgaggacacggctgtttattattgt gtgagcgatagagaactccattaccagcatttggacgtc IGG1 26 212 19 7 10 13 9 13 6 1 13 6 JY8QFUQ01CR76J 19839 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*02 F or Homsap IGHV4-39*05 F (see comment) 785 84.16 186/221 nt 83.71 185/221 nt Homsap IGHJ4*02 F (see comment) 204 91.67 44/48 nt Homsap IGHD3-3*01 F 3 10 7 16 10.7.16 [1.17.38.11] CARHRSFGVIIVNYFESW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (84,16% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) in CDR2-IMGT at codon 59 of V-REGION: 'C' (1 nucleotides at position 96 to 96 in the user submitted sequence), (cause frameshift); tttccggtgactccatcaccagtactaattattactggggctgggtccgccagtccccagggaagggtctggagtggattggaagtgtctattatCggggggacccagtacctcaacccgtctctccacaatcgagtcaccatatccattggcacgtccaagacccaattctccatgagactgacctctgtgaccgccgcagacacggctgtatatttctgtgcgagacatcggtcttttggagtgataattgttaactactttgagtcctggggacaggggaccctggtcaccgtctcctcagcttccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 93 100 [302] c75>t|t78>c|g83>a,G28>D(- - -)|g92>c,S31>T(+ - +)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c111>t|a124>g,I42>V(+ - +)|c133>t,P45>S(+ - -)|g147>t|g162>a|a166>g,I56>V(+ - +)|a175>g,S59>G(+ + -)|t177>g,S59>G(+ + -)|g191>c,S64>T(+ - +)|a193>c,T65>Q(- - -)|c194>a,T65>Q(- - -)|c195>g,T65>Q(- - -)|t199>c,Y67>L(- - -)|a200>t,Y67>L(- - -)|c210>t|a214>c,K72>H(- - +)|g216>c,K72>H(- - +)|g221>a,S74>N(- - -)|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|a242>g,D81>G(- - -)|a254>c,N85>T(- + -)|g258>a|c265>a,L89>M(+ + -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|g275>c,S92>T(+ - +)|g303>a|a308>t,Y103>F(- + -)| c75>t|t78>c| g83>a,G28>D(- - -)|g92>c,S31>T(+ - +)|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c111>t| a124>g,I42>V(+ - +)|c133>t,P45>S(+ - -)|g147>t|g162>a| a166>g,I56>V(+ - +)|a175>g,S59>G(+ + -)|t177>g,S59>G(+ + -)|g191>c,S64>T(+ - +)|a193>c,T65>Q(- - -)|c194>a,T65>Q(- - -)|c195>g,T65>Q(- - -)| t199>c,Y67>L(- - -)|a200>t,Y67>L(- - -)|c210>t|a214>c,K72>H(- - +)|g216>c,K72>H(- - +)|g221>a,S74>N(- - -)|g238>a,V80>I(+ - +)|a240>t,V80>I(+ - +)|a242>g,D81>G(- - -)|a254>c,N85>T(- + -)|g258>a|c265>a,L89>M(+ + -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|g275>c,S92>T(+ - +)|g303>a|a308>t,Y103>F(- + -)| 320 229 194 35 8 27 5 8 4 2 3 2 3 0 5 1 1 1 78 5 3 2 2 0 0 0 1 1 0 0 0 0 0 0 0 0 36 30 25 5 1 4 0 2 1 0 0 0 0 0 2 0 0 0 51 51 47 4 2 2 1 1 1 0 0 0 0 0 0 0 1 0 30 21 14 7 0 7 2 0 0 0 1 1 0 0 1 1 0 1 117 114 97 17 3 14 2 5 1 1 2 1 3 0 2 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tttcc ggtgactccatcaccagtactaattattac tggggctgggtccgccagtccccagggaagggtctggagtggattggaagt gtctattatggggggacccag tacctcaacccgtctctccacaatcgagtcaccatatccattggcacgtccaagacccaattctccatgagactgacctctgtgaccgccgcagacacggctgtatatttctgt gcgagacatcggtcttttggagtgataattgttaactactttgagtcc ARHRSFGVIIVNYFES IGHV4-39 IGHD3-3 IGHJ4 ggtgactccatcaccagtactaattattac tggggctgggtccgccagtccccagggaagggtctggagtggattggaagt gtctattatggggggacccag tacctcaacccgtctctccacaatcgagtcaccatatccattggcacgtccaagacccaattctccatgagactgacctctgtgaccgccgcagacacggctgtatatttctgt gcgagacatcggtcttttggagtgataattgttaactactttgagtcc IGG1 33 216 17 16 11 20 6 13 5 1 16 11 JY8QFUQ01CR7U0 7008 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 822 87.38 187/214 nt 86.45 185/214 nt Homsap IGHJ4*02 F (see comment) 204 91.67 44/48 nt Homsap IGHD3-10*01 F 3 8 7 16 8.7.16 [1.17.37.11] CAKESLVRGVISYYFDDW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) in CDR2-IMGT, from codon 58 of V-REGION: 3 nucleotides (from position 87 in the user submitted sequence), (do not cause frameshift)in FR3-IMGT, from codon 76 of V-REGION: 1 nucleotides (from position 131 in the user submitted sequence), (cause frameshift) cctctggattcaactttggcatctataccatggcctgggtccgccaggctccagggaaggggctggagtgggtctcagctattcgtgatcatgatagcacatactacgcagactccgtgcagggccggttttcatctcgagagacaatttcaataatacattgtatctgcaaatggatggcctgcgagccgacgacacggccgtctattactgtgcgaaagagtctctggttcggggagtcatctcatactactttgatgactggggccaggggaccctggtcaccgtctcctccgcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [293, 292] c86>a,T29>N(- + -)|a103>g,S35>G(+ + -)|g107>t,S36>I(- - -)|g112>a,A38>T(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a169>c,S57>R(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g184>c,G62>H(+ - -)|g185>a,G62>H(+ - -)|g188>a,G63>D(- - -)|a214>c,K72>Q(+ - -)|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|c237>g|c248>t,S83>F(- - -)|g252>t,K84>N(+ - -)|c255>t|g258>a|c259>t|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|a277>g,S93>G(+ + -)|a283>c|g291>c,E97>D(+ - +)|a303>c| c86>a,T29>N(- + -)|a103>g,S35>G(+ + -)|g107>t,S36>I(- - -)|g112>a,A38>T(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)| a169>c,S57>R(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g184>c,G62>H(+ - -)|g185>a,G62>H(+ - -)|g188>a,G63>D(- - -)| a214>c,K72>Q(+ - -)|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|c237>g|c248>t,S83>F(- - -)|g252>t,K84>N(+ - -)|c255>t|g258>a|c259>t|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|a277>g,S93>G(+ + -)|a283>c|g291>c,E97>D(+ - +)|a303>c| 320 222 195 27 6 21 5 5 5 0 4 1 1 0 3 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 0 0 0 1 0 0 0 0 1 0 51 51 49 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 30 21 15 6 0 6 1 3 0 0 1 0 0 0 1 0 0 0 117 113 98 15 6 9 2 1 5 0 3 0 1 0 1 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaactttggcatctatacc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcagct attcgtgatcatgatagcaca tactacgcagactccgtgcagggccggttttcatctcgagagacaatttcaataatacattgtatctgcaaatggatggcctgcgagccgacgacacggccgtctattactgt gcgaaagagtctctggttcggggagtcatctcatactactttgatgac AKESLVRGVISYYFDD IGHV3-23 IGHD3-10 IGHJ4 ggattcaactttggcatctatacc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcagct attcgtgatcatgatagcaca tactacgcagactccgtgcagggccggttttcatctcgagagacaatttcaataatacattgtatctgcaaatggatggcctgcgagccgacgacacggccgtctattactgt gcgaaagagtctctggttcggggagtcatctcatactactttgatgac IGA2 27 209 15 12 10 17 5 10 6 0 11 10 JY8QFUQ01CR8IO 17362 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 775 84.02 184/219 nt NA Homsap IGHJ6*02 F (see comment) 184 77.42 48/62 nt Homsap IGHD2-8*01 F 3 8 8 20 8.8.20 [2.17.38.11] CAKGASTAVMVSGTDCYGLDVW in-frame - Low V-REGION identity (84,02% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) or Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) gcctctggattcgccttcagtacatatatcatgaattgggtccgccaggctccagggaggggactggagtgggtcgcaactataagtggtggaggttatagtatatatgacgcagactccgtgaagggcaggttcaccatctccagagacaactccaagaccaccttgtttctggaaatgaaaagtctgagagtcgatgatacggccgtctattactgtgcgaaaggggcctcgactgcagtaatggtgtctggaacagattgttacggtttggacgtctggggccacgggaccgcggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [310] a85>g,T29>A(- - -)|t90>c|c105>t|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|g112>a,A38>I(+ - +)|c113>t,A38>I(+ - +)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a143>g,K48>R(+ + +)|g147>a|t160>g,S54>A(- + -)|g163>a,A55>T(- - -)|t168>a|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|c192>t|c194>t,T65>I(- - -)|c198>t|t199>g,Y67>D(- - -)|c223>a|t246>c|a254>c,N85>T(- + -)|g258>c|c259>t|a263>t,Y88>F(- + -)|c268>g,Q90>E(+ + -)|c276>a,N92>K(+ - -)|c279>t|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)|c294>t|a303>c| a85>g,T29>A(- - -)|t90>c|c105>t|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|g112>a,A38>I(+ - +)|c113>t,A38>I(+ - +)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a143>g,K48>R(+ + +)|g147>a|t160>g,S54>A(- + -)|g163>a,A55>T(- - -)| t168>a|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|c192>t|c194>t,T65>I(- - -)| c198>t|t199>g,Y67>D(- - -)|c223>a|t246>c|a254>c,N85>T(- + -)|g258>c|c259>t|a263>t,Y88>F(- + -)|c268>g,Q90>E(+ + -)|c276>a,N92>K(+ - -)|c279>t|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)|c294>t|a303>c| a320>g| 319 (320) 226 (227) 191 35 (36) 13 (14) 22 3 (4) 5 10 2 2 3 1 2 2 1 2 2 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 2 5 1 1 2 1 0 1 0 0 1 0 0 0 51 51 45 6 1 5 1 3 1 0 0 0 0 0 0 0 0 1 30 24 17 7 2 5 1 1 2 0 0 0 0 2 0 0 1 0 117 114 99 15 8 7 0 0 5 1 2 2 1 0 1 1 1 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcgccttcagtacatatatc atgaattgggtccgccaggctccagggaggggactggagtgggtcgcaact ataagtggtggaggttatagtata tatgacgcagactccgtgaagggcaggttcaccatctccagagacaactccaagaccaccttgtttctggaaatgaaaagtctgagagtcgatgatacggccgtctattactgt gcgaaaggggcctcgactgcagtaatggtgtctggaacagattgttacggtttggacgtc AKGASTAVMVSGTDCYGLDV IGHV3-23 IGHD2-8 IGHJ6 ggattcgccttcagtacatatatc atgaattgggtccgccaggctccagggaggggactggagtgggtcgcaact ataagtggtggaggttatagtata tatgacgcagactccgtgaagggcaggttcaccatctccagagacaactccaagaccaccttgtttctggaaatgaaaagtctgagagtcgatgatacggccgtctattactgt gcgaaaggggcctcgactgcagtaatggtgtctggaacagattgttacggtttggacgtc IGA1 35 213 20 15 15 23 5 12 9 4 12 10 JY8QFUQ01CRBJ3 16755 productive (see comment) Homsap IGHV4-39*02 F 839 86.88 192/221 nt 85.97 190/221 nt Homsap IGHJ4*02 F (see comment) 168 83.33 40/48 nt Homsap IGHD5-12*01 F 3 10 7 19 10.7.19 [1.17.38.11] CARHVVLDNRYSVWPRILDSW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 39 of V-REGION: 'G' (1 nucleotides at position 38 to 38 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 56 to 56 in the user submitted sequence), (cause frameshift); tctctggtgaccccatcggcaacactgcttactcctgGgggctggatccgccagcCccccagggaaggggctggagtggatcgcgactgtacattatgctggcagcacctactacaacccgtccctcaggagtcgagtcaccatctctgtggacacgtccaagaatcacttctccctgaagctgaattctgtgaccgcctcagacacggctgtatacttctgtgcgagacatgtggttttggacaatagatacagtgtctggcctcgtatccttgattcctggggccagggaacccttgtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 100 100 [311] g83>a,G28>D(- - -)|t85>c,S29>P(+ - -)|a91>g,S31>G(+ + -)|g101>a,S34>N(- - -)|t102>c,S34>N(- - -)|g104>c,S35>T(+ - +)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a113>c,Y38>S(+ - -)|t159>c|g161>c,G54>A(- + -)|g164>c,S55>T(+ - +)|a166>g,I56>V(+ - +)|c168>a,I56>V(+ - +)|t169>c,Y57>H(+ - -)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|g189>c|a215>g,K72>R(+ + +)|a234>c|c237>t|a240>g|c255>t|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)|g289>t,A97>S(- + -)|g303>a|t306>c|a308>t,Y103>F(- + -)| g83>a,G28>D(- - -)|t85>c,S29>P(+ - -)|a91>g,S31>G(+ + -)|g101>a,S34>N(- - -)|t102>c,S34>N(- - -)|g104>c,S35>T(+ - +)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a113>c,Y38>S(+ - -)| t159>c|g161>c,G54>A(- + -)|g164>c,S55>T(+ - +)| a166>g,I56>V(+ - +)|c168>a,I56>V(+ - +)|t169>c,Y57>H(+ - -)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|g189>c| a215>g,K72>R(+ + +)|a234>c|c237>t|a240>g|c255>t|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)|g289>t,A97>S(- + -)|g303>a|t306>c|a308>t,Y103>F(- + -)| g319>c| 318 (320) 227 (229) 198 (199) 29 (30) 8 (9) 21 6 4 3 5 2 1 1 0 6 (7) 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 21 9 0 9 2 2 0 2 1 0 0 0 2 0 0 0 51 51 48 3 1 2 0 0 0 1 0 0 0 0 2 0 0 0 30 21 15 6 1 5 2 0 0 1 0 1 0 0 2 0 0 0 117 114 103 11 6 5 2 2 3 1 1 0 1 0 0 0 1 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgaccccatcggcaacactgcttactcc tggggctggatccgccagcccccagggaaggggctggagtggatcgcgact gtacattatgctggcagcacc tactacaacccgtccctcaggagtcgagtcaccatctctgtggacacgtccaagaatcacttctccctgaagctgaattctgtgaccgcctcagacacggctgtatacttctgt gcgagacatgtggttttggacaatagatacagtgtctggcctcgtatccttgattcc ARHVVLDNRYSVWPRILDS IGHV4-39 IGHD5-12 IGHJ4 ggtgaccccatcggcaacactgcttactcc tggggctggatccgccagcccccagggaaggggctggagtggatcgcgact gtacattatgctggcagcacc tactacaacccgtccctcaggagtcgagtcaccatctctgtggacacgtccaagaatcacttctccctgaagctgaattctgtgaccgcctcagacacggctgtatacttctgt gcgagacatgtggttttggacaatagatacagtgtctggcctcgtatccttgattcc IGA2 29 216 18 11 7 15 11 14 7 1 7 14 JY8QFUQ01CRNW3 14074 productive Homsap IGHV3-11*01 F 855 88.48 192/217 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 201 88.24 45/51 nt Homsap IGHD4-17*01 F 2 8 8 17 8.8.17 [1.17.38.11] CARDGDADYGDLPIPFDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctgaattcagtttcactgaccaccacatgagctggatccgccgggctccagggaaggggctggagtgggtgtcatacattagtcctacaggtagtgccatattttacgcagactctgtgaaggcccgtttcaccatctctagggacaacgccaagaatttactatatctacaaatgaacagcctgagacccgaggacacggccatctattactgtgcgagagatggggacgcggactacggtgacttgcccatccctttcgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [299] g80>a,G27>E(- - -)|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>c,S35>T(+ - +)|t109>c,Y37>H(+ - -)|t112>c,Y38>H(+ - -)|a131>g,Q44>R(+ - -)|t159>g|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g176>c,S59>T(+ - +)|t177>a,S59>T(+ - +)|a190>g,T64>A(- - -)|a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|g221>c,G74>A(- + -)|a225>t|c237>t|c255>t|c257>t,S86>L(- - -)|g261>a|g267>a|g286>c,A96>P(- - -)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)| g80>a,G27>E(- - -)|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>c,S35>T(+ - +)|t109>c,Y37>H(+ - -)|t112>c,Y38>H(+ - -)| a131>g,Q44>R(+ - -)|t159>g| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g176>c,S59>T(+ - +)|t177>a,S59>T(+ - +)|a190>g,T64>A(- - -)| a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|g221>c,G74>A(- + -)|a225>t|c237>t|c255>t|c257>t,S86>L(- - -)|g261>a|g267>a|g286>c,A96>P(- - -)|g301>a,V101>I(+ - +)|g303>c,V101>I(+ - +)| 320 225 200 25 6 19 2 4 5 2 1 0 2 1 6 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 1 1 2 0 0 0 0 1 1 0 0 51 51 49 2 1 1 1 0 0 0 0 0 0 0 0 0 0 1 30 24 19 5 0 5 1 0 0 0 1 0 0 1 2 0 0 0 117 114 102 12 5 7 0 3 4 0 0 0 2 0 3 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gaattcagtttcactgaccaccac atgagctggatccgccgggctccagggaaggggctggagtgggtgtcatac attagtcctacaggtagtgccata ttttacgcagactctgtgaaggcccgtttcaccatctctagggacaacgccaagaatttactatatctacaaatgaacagcctgagacccgaggacacggccatctattactgt gcgagagatggggacgcggactacggtgacttgcccatccctttcgactcc ARDGDADYGDLPIPFDS IGHV3-11 IGHD4-17 IGHJ5 gaattcagtttcactgaccaccac atgagctggatccgccgggctccagggaaggggctggagtgggtgtcatac attagtcctacaggtagtgccata ttttacgcagactctgtgaaggcccgtttcaccatctctagggacaacgccaagaatttactatatctacaaatgaacagcctgagacccgaggacacggccatctattactgt gcgagagatggggacgcggactacggtgacttgcccatccctttcgactcc IGA1 25 213 13 12 9 16 4 9 6 0 8 11 JY8QFUQ01CRPMT 9811 productive (see comment) Homsap IGHV3-7*01 F 878 89.45 195/218 nt 88.99 194/218 nt Homsap IGHJ3*01 F (see comment) 151 78 39/50 nt Homsap IGHD3-16*01 F 2 8 8 8 8.8.8 [1.17.38.11] CARATNADVW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) in CDR1-IMGT at codon 35 of V-REGION: 'G' (1 nucleotides at position 19 to 19 in the user submitted sequence), (cause frameshift); cctctggattcacctttaGgggggtactggatgacctgggtccgccaggctccagggaaggggctggagtgggtggccaccataaatcacgatggaagtcaaatttactatatggaccttgtgaagggccggttcaccatctccagagacaacgccgagacctcactctatttgcaaatgaacaccctgagagccgaagacacggctgtctattactgtgcgagggcgactaatgccgacgtctggggccaagggaccacggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [274] t105>g,S35>R(- - -)|a106>g,S36>G(+ + -)|c108>g,S36>G(+ + -)|t111>c|g119>c,S40>T(+ - +)|a164>c,N55>T(- + -)|g171>t,K57>N(+ - -)|a174>c,Q58>H(- + -)|g190>c,E64>Q(+ + -)|g192>a,E64>Q(+ + -)|a194>t,K65>I(- + -)|a195>t,K65>I(- + -)|g202>a,V68>M(+ - -)|t208>c,S70>L(- - -)|c209>t,S70>L(- - -)|a225>g|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|g261>c|c265>t|g278>c,S93>T(+ - +)|g291>a|g303>c| t105>g,S35>R(- - -)|a106>g,S36>G(+ + -)|c108>g,S36>G(+ + -)|t111>c| g119>c,S40>T(+ - +)|a164>c,N55>T(- + -)| g171>t,K57>N(+ - -)|a174>c,Q58>H(- + -)|g190>c,E64>Q(+ + -)|g192>a,E64>Q(+ + -)|a194>t,K65>I(- + -)|a195>t,K65>I(- + -)| g202>a,V68>M(+ - -)|t208>c,S70>L(- - -)|c209>t,S70>L(- - -)|a225>g|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|g261>c|c265>t|g278>c,S93>T(+ - +)|g291>a|g303>c| a318>g|a320>c| 317 (320) 223 (226) 200 (201) 23 (25) 6 (8) 17 3 (4) 3 2 2 3 (4) 0 2 0 5 1 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 0 1 0 0 0 0 0 1 0 1 51 51 49 2 0 2 0 0 0 0 1 0 0 0 1 0 0 0 30 24 18 6 0 6 0 1 0 0 1 0 2 0 1 0 1 0 117 114 103 11 5 6 2 2 2 1 1 0 0 0 3 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagggggtactgg atgacctgggtccgccaggctccagggaaggggctggagtgggtggccacc ataaatcacgatggaagtcaaatt tactatatggaccttgtgaagggccggttcaccatctccagagacaacgccgagacctcactctatttgcaaatgaacaccctgagagccgaagacacggctgtctattactgt gcgagggcgactaatgccgacgtc ARATNADV IGHV3-7 IGHD3-16 IGHJ3 ggattcacctttagggggtactgg atgacctgggtccgccaggctccagggaaggggctggagtgggtggccacc ataaatcacgatggaagtcaaatt tactatatggaccttgtgaagggccggttcaccatctccagagacaacgccgagacctcactctatttgcaaatgaacaccctgagagccgaagacacggctgtctattactgt gcgagggcgactaatgccgacgtc IGA1 23 213 10 13 5 12 5 11 5 1 8 9 JY8QFUQ01CRXKV 9675 productive Homsap IGHV4-61*02 F 798 85 187/220 nt NA Homsap IGHJ4*02 F (see comment) 177 85.42 41/48 nt Homsap IGHD3-16*01 F 3 10 7 16 10.7.16 [1.17.38.11] CAREHSSSIGGERELDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) gtctggtggccccatcagcagtagtggcttcgcctggacctggatccggcagaccgccgggaagggactggagtggatcggacgtgtttatggtagtgggaataccaattacagtccttccctccagagtcgagtcaccatatcagtagagacgtccaagaatcagttctccctgaggttgacttctgtgaccgccgcagacacgggcctatattactgtgcgagagaacatagtagttcgattggcggagagagagaacttgactcctggggccagggaacccaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [299] c75>g|t85>c,S29>P(+ - -)|g103>a,G35>S(+ + -)|a106>g,S36>G(+ + -)|t108>c,S36>G(+ + -)|a110>t,Y37>F(- + -)|t112>g,Y38>A(- - -)|a113>c,Y38>A(- - -)|g119>c,S40>T(+ - +)|c133>a,P45>T(+ + -)|t159>c|g162>a|a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|a172>g,T58>G(+ - -)|c173>g,T58>G(+ - -)|c174>t,T58>G(+ - -)|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)|c198>t|a203>g,N68>S(- - -)|c204>t,N68>S(- - -)|c207>t|a214>c,K72>Q(+ - -)|c243>g,D81>E(+ - +)|c255>t|a269>g,K90>R(+ + +)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|c299>g,A100>G(- + -)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| c75>g| t85>c,S29>P(+ - -)|g103>a,G35>S(+ + -)|a106>g,S36>G(+ + -)|t108>c,S36>G(+ + -)|a110>t,Y37>F(- + -)|t112>g,Y38>A(- - -)|a113>c,Y38>A(- - -)| g119>c,S40>T(+ - +)|c133>a,P45>T(+ + -)|t159>c|g162>a| a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|a172>g,T58>G(+ - -)|c173>g,T58>G(+ - -)|c174>t,T58>G(+ - -)|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)| c198>t|a203>g,N68>S(- - -)|c204>t,N68>S(- - -)|c207>t|a214>c,K72>Q(+ - -)|c243>g,D81>E(+ - +)|c255>t|a269>g,K90>R(+ + +)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|c299>g,A100>G(- + -)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| 320 228 195 33 7 26 5 4 9 3 2 1 1 0 3 4 0 1 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 30 23 7 0 7 1 1 0 2 1 0 1 0 0 0 0 1 51 51 47 4 2 2 0 1 0 1 0 1 0 0 1 0 0 0 30 21 14 7 0 7 2 1 3 0 0 0 0 0 0 1 0 0 117 114 100 14 4 10 2 1 6 0 1 0 0 0 2 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtct ggtggccccatcagcagtagtggcttcgcc tggacctggatccggcagaccgccgggaagggactggagtggatcggacgt gtttatggtagtgggaatacc aattacagtccttccctccagagtcgagtcaccatatcagtagagacgtccaagaatcagttctccctgaggttgacttctgtgaccgccgcagacacgggcctatattactgt gcgagagaacatagtagttcgattggcggagagagagaacttgactcc AREHSSSIGGERELDS IGHV4-61 IGHD3-16 IGHJ4 ggtggccccatcagcagtagtggcttcgcc tggacctggatccggcagaccgccgggaagggactggagtggatcggacgt gtttatggtagtgggaatacc aattacagtccttccctccagagtcgagtcaccatatcagtagagacgtccaagaatcagttctccctgaggttgacttctgtgaccgccgcagacacgggcctatattactgt gcgagagaacatagtagttcgattggcggagagagagaacttgactcc IGA1 32 216 21 11 13 20 8 12 6 0 12 14 JY8QFUQ01CRXS1 3459 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 867 89.72 192/214 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 183 84.31 43/51 nt Homsap IGHD5-24*01 ORF 2 8 7 11 8.7.11 [1.17.38.11] CARDRQSWGIDSW in-frame - Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctggattcaccgtcagtaacaactacatgacctgggtccgccaggctccagggaaggggctggagtgggtctcagttctttatggcggtggtgacacatactacgcagactccgtgaagggccgattcaccatctccagagacaacgccaagaatacactttttcttcaaatgacttccctgagagtcgaagatacggccatttattactgtgcgagagatcgtcagagctgggggattgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [278] g81>a|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|a166>c,I56>L(+ + +)|a172>g,S58>G(+ + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|t246>c|t247>g,S83>A(- + -)|c255>t|g258>a|g261>t|a263>t,Y88>F(- + -)|a275>c,N92>T(- + -)|c276>t,N92>T(- + -)|a277>t|g278>c|c287>t,A96>V(+ - +)|g291>a|c294>t|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| g81>a|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)| a166>c,I56>L(+ + +)|a172>g,S58>G(+ + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| t246>c|t247>g,S83>A(- + -)|c255>t|g258>a|g261>t|a263>t,Y88>F(- + -)|a275>c,N92>T(- + -)|c276>t,N92>T(- + -)|a277>t|g278>c|c287>t,A96>V(+ - +)|g291>a|c294>t|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| 320 222 200 22 9 13 2 6 4 1 2 0 2 0 2 0 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 21 17 4 0 4 2 1 0 0 1 0 0 0 0 0 0 0 117 114 99 15 8 7 0 3 4 1 1 0 2 0 1 0 2 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccgtcagtaacaactac atgacctgggtccgccaggctccagggaaggggctggagtgggtctcagtt ctttatggcggtggtgacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaacgccaagaatacactttttcttcaaatgacttccctgagagtcgaagatacggccatttattactgt gcgagagatcgtcagagctgggggattgactcc ARDRQSWGIDS IGHV3-53 IGHD5-24 IGHJ5 ggattcaccgtcagtaacaactac atgacctgggtccgccaggctccagggaaggggctggagtgggtctcagtt ctttatggcggtggtgacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaacgccaagaatacactttttcttcaaatgacttccctgagagtcgaagatacggccatttattactgt gcgagagatcgtcagagctgggggattgactcc IGA1 22 210 13 9 10 14 3 8 8 1 8 5 JY8QFUQ01CS8O3 17983 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 994 95.83 207/216 nt 95.37 206/216 nt Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD1-26*01 F 3 8 7 12 8.7.12 [2.17.38.11] CARWRSRSYDFDYW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 57 of V-REGION: 3 nucleotides (from position 85 in the user submitted sequence), (do not cause frameshift) gcctctggattcacctttagcaactatgccatgacctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattacaagtggtggtagcacatactacgcaaactccgtgaagggccggttcaccgtctccagagacagttccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgagatggcgtagtcggagctacgactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [283] g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|c164>g,A55>G(- + -)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|t174>a,G58>T(+ - -)|g205>a,D69>N(+ + -)|a232>g,I78>V(+ - +)|a245>g,N82>S(- - -)| g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|c164>g,A55>G(- + -)| g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|t174>a,G58>T(+ - -)| g205>a,D69>N(+ + -)|a232>g,I78>V(+ - +)|a245>g,N82>S(- - -)| a317>g,K106>R(+ + +)|g319>t|a320>g| 316 (320) 220 (224) 211 (212) 9 (12) 0 (2) 9 (10) 2 (4) 3 0 0 0 0 0 1 2 1 0 (1) 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 0 0 0 0 0 0 1 1 0 0 30 21 18 3 0 3 0 1 0 0 0 0 0 1 1 0 0 0 117 114 111 3 0 3 2 1 0 0 0 0 0 0 0 0 0 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (2) 0 0 0 0 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacctttagcaactatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attacaagtggtggtagcaca tactacgcaaactccgtgaagggccggttcaccgtctccagagacagttccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagatggcgtagtcggagctacgactttgactac ARWRSRSYDFDY IGHV3-23 IGHD1-26 IGHJ4 ggattcacctttagcaactatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attacaagtggtggtagcaca tactacgcaaactccgtgaagggccggttcaccgtctccagagacagttccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagatggcgtagtcggagctacgactttgactac IGA2 9 210 5 4 3 6 2 3 0 0 5 4 JY8QFUQ01CSGBR 20350 productive (see comment) Homsap IGHV3-7*01 F 918 91.71 199/217 nt 91.24 198/217 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-3*02 F 2 8 8 14 8.8.14 [1.16.38.11] CARGSYWHYDTFGDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 55 of V-REGION: 1 nucleotides (from position 79 in the user submitted sequence), (cause frameshift) cctctggatttacttttaacaactattggatgacctgggttcgccaggctccagggaaggggctggaatgggtggccacataaaacaacatggaggtgaaacgtactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgtgcgagagggtcgtattggcattatgataccttcggcgactattggggcccgggagtcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [291] c84>t|c87>t|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|c126>t|g153>a|g171>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| c84>t|c87>t|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|c126>t|g153>a| g171>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)| a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| a320>g| 319 (320) 224 (225) 206 18 (19) 7 (8) 11 3 (4) 5 3 1 2 0 0 0 3 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 2 2 1 0 0 0 0 0 0 0 0 51 50 47 3 2 1 0 1 1 0 0 0 0 0 1 0 0 0 30 24 18 6 2 4 2 2 0 0 1 0 0 0 1 0 0 0 117 114 110 4 1 3 1 0 0 0 1 0 0 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggatttacttttaacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccac ataaaacaacatggaggtgaaacg tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat ARGSYWHYDTFGDY IGHV3-7 IGHD3-3 IGHJ4 ggatttacttttaacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccac ataaaacaacatggaggtgaaacg tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat IGG1 18 212 12 6 8 12 4 6 3 4 4 7 JY8QFUQ01CSVFI 11351 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F or Homsap IGHV3-NL1*01 F 844 88.73 189/213 nt 88.26 188/213 nt Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD2-15*01 F 3 8 7 15 8.7.15 [1.17.38.11] CTRELRVAAARDGVDIW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 59 to 59 in the user submitted sequence), (cause frameshift); tctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggGggctggagtgggtctcagtaatttatcctgatggtactacacactatggagcctccgtgaggggccggttcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgtacgagagaacttcgggtggctgctgcgcgtgatggtgttgacatctggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [290] g81>a|g88>t,V30>F(+ - -)|a109>t,N37>Y(- - -)|a113>g,Y38>W(- + -)|c114>g,Y38>W(- + -)|a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)|g132>a|t165>a|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|t196>c,Y66>H(+ - -)|c201>t|c203>g,A68>G(- + -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a225>g|t262>g,Y88>D(- - -)|g303>t|c309>t|g313>a,A105>T(- - -)| g81>a|g88>t,V30>F(+ - -)|a109>t,N37>Y(- - -)|a113>g,Y38>W(- + -)|c114>g,Y38>W(- + -)| a118>c,S40>H(+ - -)|g119>a,S40>H(+ - -)|g132>a|t165>a| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)| t196>c,Y66>H(+ - -)|c201>t|c203>g,A68>G(- + -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a225>g|t262>g,Y88>D(- - -)|g303>t|c309>t| g313>a,A105>T(- - -)| 320 221 196 25 7 18 3 5 4 1 3 0 1 1 2 2 2 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 1 1 0 0 0 0 1 0 0 1 1 0 51 51 47 4 2 2 0 2 0 0 1 0 0 1 0 0 0 0 30 21 15 6 0 6 0 1 2 0 1 0 0 0 2 0 0 0 117 114 105 9 4 5 2 0 2 1 1 0 0 0 0 1 1 1 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggagtgggtctcagta atttatcctgatggtactaca cactatggagcctccgtgaggggccggttcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttcgggtggctgctgcgcgtgatggtgttgacatc TRELRVAAARDGVDI IGHV3-53 IGHD2-15 IGHJ3 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggagtgggtctcagta atttatcctgatggtactaca cactatggagcctccgtgaggggccggttcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttcgggtggctgctgcgcgtgatggtgttgacatc IGA2 24 210 12 12 8 14 4 10 6 1 7 10 JY8QFUQ01CSWQD 12539 productive Homsap IGHV3-7*01 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD2-15*01 F 2 8 8 15 8.8.15 [1.17.38.11] CARDPRSVSRYSLFDSW in-frame - Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcacctttagtgactattggatgagctgggtccgccaggctccagggaaggggctagagtgggtggccaacataaatagagatggaagtgagcaacactatgtggactctgtgaagggccgattcaccatctccagagacgacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacatggctatgtatttttgtgcgagagatcctcgttccgtgagcaggtactctttatttgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [293] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g150>a|g171>t,K57>N(+ - -)|c172>a,Q58>R(+ - -)|a173>g,Q58>R(+ - -)|a193>c,K65>Q(+ - -)|t196>c,Y66>H(+ - -)|a244>g,N82>D(+ + -)|a254>g,N85>S(- - -)|c296>t,T99>M(- - -)|g301>a,V101>M(+ - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g150>a| g171>t,K57>N(+ - -)|c172>a,Q58>R(+ - -)|a173>g,Q58>R(+ - -)|a193>c,K65>Q(+ - -)| t196>c,Y66>H(+ - -)|a244>g,N82>D(+ + -)|a254>g,N85>S(- - -)|c296>t,T99>M(- - -)|g301>a,V101>M(+ - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| 320 225 211 14 1 13 4 3 2 1 1 1 1 0 0 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 0 0 1 1 0 0 0 0 1 0 117 114 107 7 0 7 2 1 2 1 0 0 1 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtgactattgg atgagctgggtccgccaggctccagggaaggggctagagtgggtggccaac ataaatagagatggaagtgagcaa cactatgtggactctgtgaagggccgattcaccatctccagagacgacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacatggctatgtatttttgt gcgagagatcctcgttccgtgagcaggtactctttatttgactcc ARDPRSVSRYSLFDS IGHV3-7 IGHD2-15 IGHJ4 ggattcacctttagtgactattgg atgagctgggtccgccaggctccagggaaggggctagagtgggtggccaac ataaatagagatggaagtgagcaa cactatgtggactctgtgaagggccgattcaccatctccagagacgacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacatggctatgtatttttgt gcgagagatcctcgttccgtgagcaggtactctttatttgactcc IGA2 14 213 10 4 5 7 5 7 1 0 7 6 JY8QFUQ01CT0HD 1472 productive (see comment) Homsap IGHV3-7*02 F 917 92.09 198/215 nt 91.16 196/215 nt Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD5-12*01 F 1 7 8 9 7.8.9 [1.16.38.11] CARDQVTEDYW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT, from codon 29 of V-REGION: 1 nucleotides (from position 11 in the user submitted sequence), (cause frameshift)in FR2-IMGT, from codon 40 of V-REGION: 1 nucleotides (from position 33 in the user submitted sequence), (cause frameshift) ctctggattccttttagaacctattggatgagttggtccgccaggctccagggaaagggctggagtgggtggcccacataaaccaagatggacgtgaggcatactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgtgccagagatcaagtgactgaagactactggggccagggagccctggtcaccgtcttctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [274] c87>t|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|g122>t,W41>L(+ - -)|a163>c,N55>H(- - -)|g171>c,K57>N(+ - -)|a187>c,S63>R(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)|c248>g,A83>G(- + -)|c255>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|g297>c|g301>a,V101>M(+ - -)|c309>t|g315>c| c87>t|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)| g122>t,W41>L(+ - -)|a163>c,N55>H(- - -)| g171>c,K57>N(+ - -)|a187>c,S63>R(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)| c248>g,A83>G(- + -)|c255>t|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c279>t|g297>c|g301>a,V101>M(+ - -)|c309>t| g315>c| 318 221 203 18 6 12 1 2 4 0 3 0 0 1 4 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 23 20 3 1 2 0 0 1 0 0 0 0 1 1 0 0 0 51 50 48 2 0 2 0 0 0 0 1 0 0 0 0 0 1 0 30 24 20 4 0 4 1 0 0 0 2 0 0 0 1 0 0 0 117 114 106 8 4 4 0 2 3 0 0 0 0 0 1 2 0 0 6 6 5 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattccttttagaacctattgg atgagttggtccgccaggctccagggaaagggctggagtgggtggcccac ataaaccaagatggacgtgaggca tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac ARDQVTEDY IGHV3-7 IGHD5-12 IGHJ4 ggattccttttagaacctattgg atgagttggtccgccaggctccagggaaagggctggagtgggtggcccac ataaaccaagatggacgtgaggca tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaattcagtatatctgcaaatgaacagtctgagagccgaggacaccgctatgtattattgt gccagagatcaagtgactgaagactac IGG2 17 211 7 10 6 12 1 5 4 1 6 6 JY8QFUQ01CT3CN 6889 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 890 90.7 195/215 nt 90.23 194/215 nt Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-9*01 F 2 8 8 10 8.8.10 [1.17.37.11] CVSDLAGQKGFW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 88 of V-REGION: 1 nucleotides (from position 167 in the user submitted sequence), (cause frameshift) tcaggattcaccttcagtaggtactggatgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgtattaatgaagacggcagctacacagatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgttttgcaaatgaacagtctgagaggcgaggacacggctgtctattactgtgttagcgatttggctggtcagaaaggtttctggggccagggagtcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [276] t78>a|c108>g,S36>R(- - -)|c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|c265>t|c287>g,A96>G(- + -)|g303>c| t78>a| c108>g,S36>R(- - -)| c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|c265>t|c287>g,A96>G(- + -)|g303>c| c314>t,A105>V(+ - +)|a315>t,A105>V(+ - +)|a318>c,R106>S(- - -)| 320 223 200 23 6 17 2 3 4 4 1 1 2 2 2 2 0 0 78 3 2 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 49 2 0 2 0 0 1 1 0 0 0 0 0 0 0 0 30 24 16 8 3 5 1 2 0 2 0 0 1 1 1 0 0 0 117 113 105 8 2 6 1 1 2 1 0 1 0 0 1 1 0 0 8 8 5 3 0 3 0 0 1 0 1 0 1 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tca ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagctacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgttttgcaaatgaacagtctgagaggcgaggacacggctgtctattactgt gttagcgatttggctggtcagaaaggtttc VSDLAGQKGF IGHV3-74 IGHD3-9 IGHJ4 ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagctacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgttttgcaaatgaacagtctgagaggcgaggacacggctgtctattactgt gttagcgatttggctggtcagaaaggtttc IGA1 19 212 12 7 6 11 6 8 2 3 8 6 JY8QFUQ01CTI25 16991 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 999 95.85 208/217 nt NA Homsap IGHJ5*02 F 237 96.08 49/51 nt Homsap IGHD6-6*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVRSEYSSSNWFDPW in-frame - ctctggattcaccttcagtagctatagaatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattgatagtagtagtcacaacatatactacagagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgtgagatccgagtatagcagttcaaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [287] c114>a,S38>R(- - -)|a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a187>c,S63>H(+ - -)|g188>a,S63>H(+ - -)|t189>c,S63>H(+ - -)|t190>a,Y64>N(- - -)|g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|c314>t,A105>V(+ - +)| c114>a,S38>R(- - -)| a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a187>c,S63>H(+ - -)|g188>a,S63>H(+ - -)|t189>c,S63>H(+ - -)|t190>a,Y64>N(- - -)| g202>a,A68>R(- - -)|c203>g,A68>R(- - -)| c314>t,A105>V(+ - +)|g319>t|a320>c| 318 (320) 223 (225) 213 10 (12) 0 (2) 10 1 3 1 1 1 (2) 1 0 1 0 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 18 6 0 6 1 2 0 1 1 0 0 1 0 0 0 0 117 114 112 2 0 2 0 1 0 0 0 0 0 0 0 1 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 0 1 0 0 (1) 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctataga atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attgatagtagtagtcacaacata tactacagagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gtgagatccgagtatagcagttcaaactggttcgacccc VRSEYSSSNWFDP IGHV3-21 IGHD6-6 IGHJ5 ggattcaccttcagtagctataga atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attgatagtagtagtcacaacata tactacagagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gtgagatccgagtatagcagttcaaactggttcgacccc IGA1 9 213 5 4 3 5 2 4 0 0 2 7 JY8QFUQ01CTJ46 8058 productive Homsap IGHV3-30*01 F 815 86.24 188/218 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 156 78.43 40/51 nt Homsap IGHD5-12*01 F 3 8 8 12 8.8.12 [1.17.38.11] CARDFDTDDVGTYW in-frame - Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) tctctggattcatcttcgatagttatgctctgcactgggtccgccaggctccaggcaaggggctagagtgggtggcacttgtttcatatgatggaaaatataagcaatatgcagattctgtggagggccgattcaccatctccagagacaactccaagaacacaggatatctgcaaatgaacagcctgacatctgacgatacggctgtgtatttttgtgcgagagacttcgatactgacgacgtgggcacctactggggccggggaaccgtggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [285, 284] c74>t|c86>t,T29>I(- - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c108>t|a115>c,M39>L(+ + -)|g163>c,V55>L(+ - +)|a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|g188>a,S63>K(- - -)|t189>a,S63>K(- - -)|a190>t,N64>Y(- - -)|a195>g|t196>c,Y66>Q(- - -)|c198>a,Y66>Q(- - -)|c201>t|c207>t|c210>t|a214>g,K72>E(+ - -)|t246>c|g258>a|c259>g,L87>G(- - -)|t260>g,L87>G(- - -)|g261>a,L87>G(- - -)|g284>c,R95>T(- - -)|g286>t,A96>S(- + -)|g291>c,E97>D(+ - +)|c294>t|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| c74>t| c86>t,T29>I(- - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c108>t| a115>c,M39>L(+ + -)|g163>c,V55>L(+ - +)| a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|g188>a,S63>K(- - -)|t189>a,S63>K(- - -)|a190>t,N64>Y(- - -)|a195>g| t196>c,Y66>Q(- - -)|c198>a,Y66>Q(- - -)|c201>t|c207>t|c210>t|a214>g,K72>E(+ - -)|t246>c|g258>a|c259>g,L87>G(- - -)|t260>g,L87>G(- - -)|g261>a,L87>G(- - -)|g284>c,R95>T(- - -)|g286>t,A96>S(- + -)|g291>c,E97>D(+ - +)|c294>t|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| 320 226 196 30 9 21 4 4 8 2 1 1 3 1 3 1 1 1 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 2 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 0 0 1 0 0 0 1 0 0 0 30 24 18 6 1 5 2 1 0 0 0 0 2 1 0 0 0 0 117 114 97 17 6 11 1 2 5 2 0 1 1 0 2 1 1 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| tctct ggattcatcttcgatagttatgct ctgcactgggtccgccaggctccaggcaaggggctagagtgggtggcactt gtttcatatgatggaaaatataag caatatgcagattctgtggagggccgattcaccatctccagagacaactccaagaacacaggatatctgcaaatgaacagcctgacatctgacgatacggctgtgtatttttgt gcgagagacttcgatactgacgacgtgggcacctac ARDFDTDDVGTY IGHV3-30 IGHD5-12 IGHJ5 ggattcatcttcgatagttatgct ctgcactgggtccgccaggctccaggcaaggggctagagtgggtggcactt gtttcatatgatggaaaatataag caatatgcagattctgtggagggccgattcaccatctccagagacaactccaagaacacaggatatctgcaaatgaacagcctgacatctgacgatacggctgtgtatttttgt gcgagagacttcgatactgacgacgtgggcacctac IGA2 29 213 17 12 11 17 6 12 6 2 13 8 JY8QFUQ01CU8BS 6127 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 935 93.02 200/215 nt 92.09 198/215 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 3 8 7 12 8.7.12 [1.16.38.11] CARDTGQWKDFDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 49 of V-REGION: 1 nucleotides (from position 60 in the user submitted sequence), (cause frameshift)in CDR2-IMGT, from codon 58 of V-REGION: 1 nucleotides (from position 86 in the user submitted sequence), (cause frameshift) ctctggattcatcttcagtagctactggatgcactgggtccgccaagctccagggaagggctggtgtgggtgtcacgtagtaatacggggggactgacacagcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgtgcaagagatacggggcagtggaaggactttgactactggggccagggaaccctggtcaccgtctccttagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtcagggcttcttccc NA IGA1 100 100 [282] c86>t,T29>I(- - -)|c159>g|t167>g,I56>S(- - -)|t174>c|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| c86>t,T29>I(- - -)| c159>g| t167>g,I56>S(- - -)|t174>c|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| 320 223 208 15 4 11 3 1 1 1 1 0 0 0 2 3 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 50 49 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 30 23 16 7 1 6 2 1 0 1 0 0 0 0 1 0 0 2 117 114 108 6 2 4 1 0 0 0 1 0 0 0 1 2 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaagggctggtgtgggtgtcacgt agtaatacggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac ARDTGQWKDFDY IGHV3-74 IGHD6-19 IGHJ4 ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaagggctggtgtgggtgtcacgt agtaatacggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac IGA1 15 211 6 9 2 8 4 7 3 1 4 7 JY8QFUQ01CU8RS 18255 productive (see comment) Homsap IGHV3-66*01 F, or Homsap IGHV3-66*02 F or Homsap IGHV3-66*04 F 948 93.93 201/214 nt 93.46 200/214 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD4-23*01 ORF 2 8 7 10 8.7.10 [1.17.38.11] CARGDGVKDDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'G' (1 nucleotides at position 21 to 21 in the user submitted sequence), (cause frameshift); ctctggattcaccgtcaataGgaaactacatgagctgggtccgccaggctccagggaagggactggagtgggtctcagttatttccagcggtggttccacatactacgcaaactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtatatcttcaaatgaacagcctgagagtcgacgacacggctgtgtattactgtgcgagaggggacggggttaaggatgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [277] g104>a,S35>N(- - -)|c108>a,S36>R(- - -)|g147>a|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a190>t|g191>c|g205>a,D69>N(+ + -)|a223>c|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| g104>a,S35>N(- - -)|c108>a,S36>R(- - -)| g147>a| a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a190>t|g191>c| g205>a,D69>N(+ + -)|a223>c|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| a320>g| 319 (320) 221 (222) 208 13 (14) 4 (5) 9 0 (1) 4 1 1 2 1 1 0 2 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 1 0 0 0 0 0 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 21 17 4 2 2 0 0 0 1 1 0 1 0 1 0 0 0 117 114 108 6 1 5 0 2 1 0 1 0 0 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|ggca,220-223(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccgtcaatagaaactac atgagctgggtccgccaggctccagggaagggactggagtgggtctcagtt atttccagcggtggttccaca tactacgcaaactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtatatcttcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaggggacggggttaaggatgactac ARGDGVKDDY IGHV3-66 IGHD4-23 IGHJ4 ggattcaccgtcaatagaaactac atgagctgggtccgccaggctccagggaagggactggagtgggtctcagtt atttccagcggtggttccaca tactacgcaaactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtatatcttcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaggggacggggttaaggatgactac IGG2 13 210 6 7 5 9 1 4 2 2 5 4 JY8QFUQ01CVKGA 6970 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-3*01 F 1 8 8 10 8.8.10 [1.17.38.11] CARGGLEPVDYW in-frame + ctctggattcaccttcagtacatactggatgcactgggtccaccaagctccagggaaggggctggtgtgggtctcccgtatcaatcctgatgggcgaatcacaaactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggactcggctgtgtactactgtgcaaggggtggtttggagcccgtagattattggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctggctgcctggtcaaggactacttccccgaaccgtgacggtgtcgtggaactcag NA IGG2 100 100 [279] g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|g128>a,R43>H(- - +)|a162>c|t168>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|g216>t,K72>N(+ - -)|a295>t,T99>S(+ - +)|t306>c| g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)| g128>a,R43>H(- - +)|a162>c| t168>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|a187>c,S63>R(- - -)|t189>a,S63>R(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|g216>t,K72>N(+ - -)|a295>t,T99>S(+ - +)|t306>c| a318>g|a320>g| 317 (320) 222 (225) 208 (209) 14 (16) 3 (5) 11 0 (2) 2 0 2 3 1 1 1 2 0 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 1 0 0 1 0 0 0 51 51 49 2 1 1 0 1 0 0 1 0 0 0 0 0 0 0 30 24 18 6 1 5 0 0 0 1 2 0 0 1 1 0 1 0 117 114 110 4 1 3 0 1 0 1 0 0 1 0 0 0 1 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtacatactgg atgcactgggtccaccaagctccagggaaggggctggtgtgggtctcccgt atcaatcctgatgggcgaatcaca aactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggactcggctgtgtactactgt gcaaggggtggtttggagcccgtagattat ARGGLEPVDY IGHV3-74 IGHD3-3 IGHJ4 ggattcaccttcagtacatactgg atgcactgggtccaccaagctccagggaaggggctggtgtgggtctcccgt atcaatcctgatgggcgaatcaca aactacgcggactccgtgaatggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagccgaggactcggctgtgtactactgt gcaaggggtggtttggagcccgtagattat IGG2 14 213 4 10 2 7 2 7 2 1 4 7 JY8QFUQ01CVRND 6070 productive (see comment) Homsap IGHV3-33*05 F (see comment) 762 83.18 183/220 nt 82.73 182/220 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-12*01 F 3 8 8 13 8.8.13 [2.17.38.11] CVKGRGYNDYGLDYW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (83,18% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR2-IMGT at codon 62 of V-REGION: 'A' (1 nucleotides at position 95 to 95 in the user submitted sequence), (cause frameshift); agcgtctggattctccttcagcaattatgccatccactgggtccgccaggctccaggcaaggggctggagtgggtggcgaccatttcatatgatAttaatgaaaagatattatgcagagtccgtgaggggccgattcaccctctccagagacaattccaagaacactctcgatctgctcatggatacccttcggttcgacgacacggctgtctattattgtgttaaaggacgtggatacaatgactacggcctcgactactggggcccgggaaccctgctcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [292] a85>t,T29>S(+ - +)|t105>c|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g113>c,G38>A(- + -)|g117>c,M39>I(+ + -)|a162>g|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)|t165>c,V55>T(- - -)|a168>t|g184>t,G62>L(- - -)|g185>t,G62>L(- - -)|g188>t,S63>M(- - -)|t189>g,S63>M(- - -)|t192>a,N64>K(+ - -)|a194>g,K65>R(+ + +)|c198>t|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|a232>c,I78>L(+ + +)|g258>t|g261>c|t262>g,Y88>D(- - -)|a269>t,Q90>L(- - -)|a270>c,Q90>L(- - -)|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|g278>c,S93>T(+ - +)|g282>t|a283>c|a285>g|g286>t,A96>F(+ - -)|c287>t,A96>F(+ - -)|g291>c,E97>D(+ - +)|g303>c|c309>t| a85>t,T29>S(+ - +)|t105>c|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g113>c,G38>A(- + -)| g117>c,M39>I(+ + -)|a162>g|g163>a,V55>T(- - -)|t164>c,V55>T(- - -)|t165>c,V55>T(- - -)| a168>t|g184>t,G62>L(- - -)|g185>t,G62>L(- - -)|g188>t,S63>M(- - -)|t189>g,S63>M(- - -)|t192>a,N64>K(+ - -)|a194>g,K65>R(+ + +)| c198>t|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|a232>c,I78>L(+ + +)|g258>t|g261>c|t262>g,Y88>D(- - -)|a269>t,Q90>L(- - -)|a270>c,Q90>L(- - -)|a274>g,N92>D(+ + -)|c276>t,N92>D(+ + -)|g278>c,S93>T(+ - +)|g282>t|a283>c|a285>g|g286>t,A96>F(+ - -)|c287>t,A96>F(+ - -)|g291>c,E97>D(+ - +)|g303>c|c309>t| c314>t,A105>V(+ - +)|g315>t,A105>V(+ - +)|g317>a,R106>K(+ + +)|a320>g| 319 (320) 227 (228) 187 40 (41) 11 (12) 29 5 (6) 3 6 3 3 0 3 1 6 1 7 2 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 1 1 0 0 1 0 1 0 0 0 51 51 46 5 1 4 1 1 0 2 0 0 0 0 1 0 0 0 30 24 17 7 1 6 1 0 0 0 0 0 1 1 0 0 3 1 117 114 94 20 8 12 3 0 4 0 3 0 1 0 4 1 3 1 7 (8) 7 4 3 (4) 0 (1) 3 0 (1) 1 1 0 0 0 0 0 0 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcgtct ggattctccttcagcaattatgcc atccactgggtccgccaggctccaggcaaggggctggagtgggtggcgacc atttcatatgatttaatgaaaaga tattatgcagagtccgtgaggggccgattcaccctctccagagacaattccaagaacactctcgatctgctcatggatacccttcggttcgacgacacggctgtctattattgt gttaaaggacgtggatacaatgactacggcctcgactac VKGRGYNDYGLDY IGHV3-33 IGHD5-12 IGHJ4 ggattctccttcagcaattatgcc atccactgggtccgccaggctccaggcaaggggctggagtgggtggcgacc atttcatatgatttaatgaaaaga tattatgcagagtccgtgaggggccgattcaccctctccagagacaattccaagaacactctcgatctgctcatggatacccttcggttcgacgacacggctgtctattattgt gttaaaggacgtggatacaatgactacggcctcgactac IGG1 37 213 15 22 7 20 8 17 9 2 16 10 JY8QFUQ01CVY8N 14178 productive Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F or Homsap IGHV4-34*07 F 845 88.37 190/215 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD6-13*01 F 2 8 7 8 8.7.8 [1.17.38.11] CARISAFDQW in-frame + tacacggcgggtccttcaaaggttactattggagctggatccgccagcccccaggaaaggggctggagtggattggagaaatcgaccatagtggaaacaccaactacaacccgtccctcaagagtcgagtcaccgtgacagttgacacatccaagaaccaaatttccctgaatttgacctctgtgaccgccgcggacacggctatttattactgtgcgcgtatttcagcctttgaccagtggggccagggaaccccggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccg NA IGA1 100 100 [270] c75>a|t76>c,Y26>H(+ - -)|t78>c,Y26>H(+ - -)|t81>c|g104>a,S35>K(- - -)|t105>a,S35>K(- - -)|c114>t|g141>a|g162>a|a169>g,N57>D(+ + -)|t171>c,N57>D(+ + -)|g191>a,S64>N(- - -)|a232>g,I78>V(+ - +)|a234>g,I78>V(+ - +)|t235>a,S79>T(+ - +)|a240>t|g246>a|g258>a|t259>a,F87>I(+ - -)|c261>t,F87>I(+ - -)|g270>t,K90>N(+ - -)|c271>t|g275>c,S92>T(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| c75>a|t76>c,Y26>H(+ - -)|t78>c,Y26>H(+ - -)| t81>c|g104>a,S35>K(- - -)|t105>a,S35>K(- - -)|c114>t| g141>a|g162>a| a169>g,N57>D(+ + -)|t171>c,N57>D(+ + -)|g191>a,S64>N(- - -)| a232>g,I78>V(+ - +)|a234>g,I78>V(+ - +)|t235>a,S79>T(+ - +)|a240>t|g246>a|g258>a|t259>a,F87>I(+ - -)|c261>t,F87>I(+ - -)|g270>t,K90>N(+ - -)|c271>t|g275>c,S92>T(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| a316>c|a318>t|g319>a|g320>t| 315 (320) 218 (223) 193 (194) 25 (29) 9 (13) 16 3 7 (8) 3 4 0 (1) 1 1 (2) 3 1 0 2 (3) 0 78 5 2 3 1 2 0 0 0 2 0 1 0 0 0 0 0 0 36 24 20 4 2 2 0 1 1 1 0 0 0 1 0 0 0 0 51 51 49 2 2 0 0 2 0 0 0 0 0 0 0 0 0 0 30 21 18 3 0 3 1 1 0 1 0 0 0 0 0 0 0 0 117 114 101 13 4 9 2 3 2 0 0 0 1 2 1 0 2 0 3 (8) 3 3 (4) 0 (4) 0 (4) 0 0 0 (1) 0 0 0 (1) 0 0 (1) 0 0 0 0 (1) 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tacac ggcgggtccttcaaaggttactat tggagctggatccgccagcccccaggaaaggggctggagtggattggagaa atcgaccatagtggaaacacc aactacaacccgtccctcaagagtcgagtcaccgtgacagttgacacatccaagaaccaaatttccctgaatttgacctctgtgaccgccgcggacacggctatttattactgt gcgcgtatttcagcctttgaccag ARISAFDQ IGHV4-34 IGHD6-13 IGHJ4 ggcgggtccttcaaaggttactat tggagctggatccgccagcccccaggaaaggggctggagtggattggagaa atcgaccatagtggaaacacc aactacaacccgtccctcaagagtcgagtcaccgtgacagttgacacatccaagaaccaaatttccctgaatttgacctctgtgaccgccgcggacacggctatttattactgt gcgcgtatttcagcctttgaccag IGA1 22 210 15 7 10 13 5 9 6 2 9 5 JY8QFUQ01CW65U 7561 productive Homsap IGHV3-7*01 F 949 93.52 202/216 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD5-18*01 F 3 8 8 15 8.8.15 [1.17.38.11] CARVGYGYRGIYFFDYW in-frame + tctacattcacgtttagtcgatattggatgagctgggtccgccaggctccagggaagggcctggagtgggtggccaacataaaggaagacggaagtgagagatattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgtgcgagagtgggctatggttaccgaggaatatacttctttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG2 100 100 [293] g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|c87>g|a106>c,S36>R(- - -)|c108>a,S36>R(- - -)|g147>c|c172>g,Q58>E(+ + -)|t177>c|a194>g,K65>R(+ + +)|c198>t|c246>t|a258>t|a271>t,M91>L(+ + -)|c280>t| g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|c87>g|a106>c,S36>R(- - -)|c108>a,S36>R(- - -)| g147>c| c172>g,Q58>E(+ + -)|t177>c|a194>g,K65>R(+ + +)| c198>t|c246>t|a258>t|a271>t,M91>L(+ + -)|c280>t| a320>t| 319 (320) 223 (224) 209 14 (15) 7 (8) 7 1 1 3 1 1 1 2 (3) 0 2 2 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 0 0 1 1 0 0 1 1 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 21 3 1 2 1 0 0 1 0 0 0 0 0 1 0 0 117 114 109 5 4 1 0 0 3 0 0 0 2 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct acattcacgtttagtcgatattgg atgagctgggtccgccaggctccagggaagggcctggagtgggtggccaac ataaaggaagacggaagtgagaga tattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgt gcgagagtgggctatggttaccgaggaatatacttctttgactac ARVGYGYRGIYFFDY IGHV3-7 IGHD5-18 IGHJ4 acattcacgtttagtcgatattgg atgagctgggtccgccaggctccagggaagggcctggagtgggtggccaac ataaaggaagacggaagtgagaga tattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgt gcgagagtgggctatggttaccgaggaatatacttctttgactac IGG2 14 213 6 8 4 9 2 5 5 2 1 6 JY8QFUQ01CWEVR 17175 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-8*01 F 2 8 8 16 8.8.16 [1.17.38.11] CARDRGCGNGACHVADYW in-frame + ctctggattcaccttcagtaactatagtatgaactgggtccgccaggctccagggaagggcctggagtgggtctcatccatcagtagtggtggtagtttcaaacaccacgcagactcagcgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattattgtgcgagagatcggggttgtggtaatggtgcatgccatgtcgccgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccc NA IGA1 71 100 [296] g107>a,S36>N(- - -)|c114>t|g147>c|t168>c|a175>g,S59>G(+ + -)|a184>g,S62>G(+ + -)|a191>t,Y64>F(- + -)|t194>a,I65>K(- + -)|t196>c,Y66>H(+ - -)|t199>c,Y67>H(+ - -)|t212>c,V71>A(+ - +)|c309>t| g107>a,S36>N(- - -)|c114>t| g147>c| t168>c|a175>g,S59>G(+ + -)|a184>g,S62>G(+ + -)|a191>t,Y64>F(- + -)|t194>a,I65>K(- + -)| t196>c,Y66>H(+ - -)|t199>c,Y67>H(+ - -)|t212>c,V71>A(+ - +)|c309>t| 320 225 213 12 4 8 2 1 2 4 0 0 1 1 1 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 19 5 1 4 2 0 0 1 0 0 1 1 0 0 0 0 117 114 110 4 1 3 0 0 1 3 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtaactatagt atgaactgggtccgccaggctccagggaagggcctggagtgggtctcatcc atcagtagtggtggtagtttcaaa caccacgcagactcagcgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattattgt gcgagagatcggggttgtggtaatggtgcatgccatgtcgccgactac ARDRGCGNGACHVADY IGHV3-21 IGHD2-8 IGHJ4 ggattcaccttcagtaactatagt atgaactgggtccgccaggctccagggaagggcctggagtgggtctcatcc atcagtagtggtggtagtttcaaa caccacgcagactcagcgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattattgt gcgagagatcggggttgtggtaatggtgcatgccatgtcgccgactac IGA1 12 213 9 3 3 4 6 8 2 2 3 5 JY8QFUQ01CWPJP 18212 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 866 88.24 195/221 nt 87.33 193/221 nt Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 192 86.27 44/51 nt Homsap IGHD3-22*01 F 2 10 7 13 10.7.13 [1.17.38.11] CARQLSGDYASFDSW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 64 to 64 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 87 of V-REGION: 'G' (1 nucleotides at position 169 to 169 in the user submitted sequence), (cause frameshift); tctctggtgcctccatcaccagtggtactttttactgggcctggatccgccagcccccagggaAaggggctggagtgggttggcaatatctattctagtggtgtcgcctattacaacccgtccctcaagagtcgagtcaccatgtccgtcgacacgtccaagaatgagGttttccctgacactgacctctgtgaccgccgcagacacggctgtatatttctgtgcgagacagctcagtggtgactacgcatcgtttgactcctggggccagggaaccctggtcgccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [293] g83>c,G28>A(- + -)|g92>c,S31>T(+ - +)|a103>g,S35>G(+ + -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|c111>t,Y37>F(- + -)|g119>c,G40>A(- + -)|a157>g,I53>V(+ - +)|g162>c|g164>a,S55>N(- - -)|a173>c,Y58>S(+ - -)|g189>t|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|a193>g,T65>A(- - -)|c198>t|a234>g,I78>M(+ + -)|a240>c|c255>t|c256>g,Q86>E(+ + -)|c261>t|a269>c,K90>T(- - -)|g270>a,K90>T(- - -)|g275>c,S92>T(+ - +)|g303>a|a308>t,Y103>F(- + -)| g83>c,G28>A(- + -)|g92>c,S31>T(+ - +)|a103>g,S35>G(+ + -)|g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|c111>t,Y37>F(- + -)| g119>c,G40>A(- + -)|a157>g,I53>V(+ - +)|g162>c|g164>a,S55>N(- - -)| a173>c,Y58>S(+ - -)|g189>t|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|a193>g,T65>A(- - -)| c198>t|a234>g,I78>M(+ + -)|a240>c|c255>t|c256>g,Q86>E(+ + -)|c261>t|a269>c,K90>T(- - -)|g270>a,K90>T(- - -)|g275>c,S92>T(+ - +)|g303>a|a308>t,Y103>F(- + -)| 320 229 203 26 7 19 5 3 4 0 3 0 2 0 6 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 0 6 1 0 1 0 0 0 1 0 3 0 0 0 51 51 47 4 1 3 1 1 0 0 0 0 0 0 2 0 0 0 30 21 16 5 1 4 2 0 0 0 1 0 0 0 0 0 2 0 117 114 103 11 5 6 1 2 3 0 2 0 1 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgcctccatcaccagtggtactttttac tgggcctggatccgccagcccccagggaaggggctggagtgggttggcaat atctattctagtggtgtcgcc tattacaacccgtccctcaagagtcgagtcaccatgtccgtcgacacgtccaagaatgagttttccctgacactgacctctgtgaccgccgcagacacggctgtatatttctgt gcgagacagctcagtggtgactacgcatcgtttgactcc ARQLSGDYASFDS IGHV4-39 IGHD3-22 IGHJ5 ggtgcctccatcaccagtggtactttttac tgggcctggatccgccagcccccagggaaggggctggagtgggttggcaat atctattctagtggtgtcgcc tattacaacccgtccctcaagagtcgagtcaccatgtccgtcgacacgtccaagaatgagttttccctgacactgacctctgtgaccgccgcagacacggctgtatatttctgt gcgagacagctcagtggtgactacgcatcgtttgactcc IGA1 26 216 12 14 7 16 5 10 6 1 9 10 JY8QFUQ01CWQZU 17450 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 963 94.01 204/217 nt NA Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD2-21*01 F 3 8 8 16 8.8.16 [1.17.38.11] CARGRIVSVGMSGWFDPW in-frame + ctctggattcactttcagtaactactggatgtactgggtccgccaagctccagggaaggggctggagtgggtctcacgtattaatggtgatggaagtagtacaagttacgtggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtttattactgtgcgagaggccgaatagtgtcggttggtatgtcaggctggttcgacccctggggccagggaaccctggtctccgtctcctcagcatccccgaccagccccaaggtcttccccgctgagccctcgacagcaccccccaagatgggaacgtggtccgtcgcatgcctggtccagggcttcttcccc NA IGA2 79 84 [298, 296] c87>t|g107>a,S36>N(- - -)|c118>t,H40>Y(+ - -)|t152>a,V51>E(- + -)|a172>g,S58>G(+ + -)|g186>a|c192>t|c198>t|c203>t,A68>V(+ - +)|g258>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>t|a315>g| c87>t|g107>a,S36>N(- - -)| c118>t,H40>Y(+ - -)|t152>a,V51>E(- + -)| a172>g,S58>G(+ + -)|g186>a|c192>t| c198>t|c203>t,A68>V(+ - +)|g258>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>t| a315>g|a320>g| 319 (320) 224 (225) 210 14 (15) 7 (8) 7 2 (3) 2 6 0 0 0 0 1 2 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 1 0 0 0 0 1 0 0 0 0 30 24 21 3 2 1 1 1 1 0 0 0 0 0 0 0 0 0 117 114 108 6 3 3 0 0 3 0 0 0 0 0 2 0 1 0 7 (8) 7 6 1 (2) 1 (2) 0 1 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcactttcagtaactactgg atgtactgggtccgccaagctccagggaaggggctggagtgggtctcacgt attaatggtgatggaagtagtaca agttacgtggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtttattactgt gcgagaggccgaatagtgtcggttggtatgtcaggctggttcgacccc ARGRIVSVGMSGWFDP IGHV3-74 IGHD2-21 IGHJ5 ggattcactttcagtaactactgg atgtactgggtccgccaagctccagggaaggggctggagtgggtctcacgt attaatggtgatggaagtagtaca agttacgtggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtttattactgt gcgagaggccgaatagtgtcggttggtatgtcaggctggttcgacccc IGA2 13 213 9 4 8 11 1 2 3 3 5 2 JY8QFUQ01CWYA5 7710 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F (see comment) 687 80.37 172/214 nt 79.91 171/214 nt Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD6-6*01 F 3 8 7 9 8.7.9 [1.17.38.11] CARLDGHFDLW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (80,37% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR3-IMGT at codon 67 of V-REGION: 'C' (1 nucleotides at position 106 to 106 in the user submitted sequence), (cause frameshift); ctctgggttcaccatcagtcactactccatggcctgggtccgccaggctccgggaaaggggctggagtgggtctcattaatttatcccggcgggagcacattccaCggcagactccgtgagggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgtgcgagactcgacgggcacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [274] g88>a,V30>I(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g|a197>t,Y66>F(- + -)|t199>c,Y67>Q(- - -)|c201>g,Y67>Q(- - -)|a215>g,K72>R(+ + +)|c222>g|a225>g|t226>c,F76>L(+ - -)|c228>t,F76>L(+ - -)|c230>t,T77>I(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| g88>a,V30>I(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g| a197>t,Y66>F(- + -)|t199>c,Y67>Q(- - -)|c201>g,Y67>Q(- - -)|a215>g,K72>R(+ + +)|c222>g|a225>g|t226>c,F76>L(+ - -)|c228>t,F76>L(+ - -)|c230>t,T77>I(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| g319>c|a320>t| 318 (320) 220 (222) 178 42 (44) 9 (11) 33 9 8 5 3 3 2 2 (3) 2 2 (3) 4 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 2 0 0 2 0 1 0 0 0 0 0 51 51 45 6 2 4 2 1 0 0 0 0 0 1 1 0 1 0 30 21 17 4 2 2 0 0 0 1 1 0 0 0 1 0 0 1 117 114 87 27 5 22 7 5 5 2 0 2 1 1 0 4 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcaccatcagtcactactcc atggcctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccaggcagactccgtgagggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc ARLDGHFDL IGHV3-53 IGHD6-6 IGHJ2 gggttcaccatcagtcactactcc atggcctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccaggcagactccgtgagggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc IGG1 42 210 25 17 13 22 12 20 7 2 26 7 JY8QFUQ01CXAGM 20894 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 864 88.94 193/217 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD6-19*01 F 1 8 8 10 8.8.10 [1.17.38.11] CMNELGYCSGGG in-frame - ttctggattcacttttagcagtcatatgatgagttgggtccgccaggctccagggaagggactggaatgggtctcaagtattcgtgccagtggtgataggacacactacgcagactccgtgaagggccgcttcaccatctccagagacaactccaagaacacgctgtatttgcaaatgtacagcctgagagtcgaggacacggccttatattactgtatgaacgaactcggatattgtagtggtggggggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA1 71 100 [278] c75>t|c87>t|c108>t|t109>c,Y37>H(+ - -)|g112>a,A38>M(+ - -)|c113>t,A38>M(+ - -)|c114>g,A38>M(+ - -)|c120>t|g147>a|g153>a|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|a169>c,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g188>a,G63>D(- - -)|c192>g,S64>R(- - -)|t196>c,Y66>H(+ - -)|g225>c|t246>c|c265>t|a274>t,N92>Y(- - -)|c287>t,A96>V(+ - +)|g301>t,V101>L(+ - +)|g313>a,A105>M(+ - -)|c314>t,A105>M(+ - -)| c75>t| c87>t|c108>t|t109>c,Y37>H(+ - -)|g112>a,A38>M(+ - -)|c113>t,A38>M(+ - -)|c114>g,A38>M(+ - -)| c120>t|g147>a|g153>a|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| a169>c,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g188>a,G63>D(- - -)|c192>g,S64>R(- - -)| t196>c,Y66>H(+ - -)|g225>c|t246>c|c265>t|a274>t,N92>Y(- - -)|c287>t,A96>V(+ - +)|g301>t,V101>L(+ - +)| g313>a,A105>M(+ - -)|c314>t,A105>M(+ - -)|a318>c,K106>N(+ - -)| 320 225 198 27 9 18 0 6 8 4 2 0 1 0 2 3 1 0 78 4 3 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 18 6 2 4 0 1 3 1 0 0 0 0 0 1 0 0 51 51 46 5 3 2 0 3 1 0 0 0 0 0 0 1 0 0 30 24 19 5 0 5 0 1 0 1 1 0 0 0 1 1 0 0 117 114 107 7 3 4 0 0 2 2 0 0 1 0 1 0 1 0 8 8 5 3 0 3 0 1 1 0 1 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ttct ggattcacttttagcagtcatatg atgagttgggtccgccaggctccagggaagggactggaatgggtctcaagt attcgtgccagtggtgataggaca cactacgcagactccgtgaagggccgcttcaccatctccagagacaactccaagaacacgctgtatttgcaaatgtacagcctgagagtcgaggacacggccttatattactgt atgaacgaactcggatattgtagtggtggg MNELGYCSGG IGHV3-23 IGHD6-19 IGHJ4 ggattcacttttagcagtcatatg atgagttgggtccgccaggctccagggaagggactggaatgggtctcaagt attcgtgccagtggtgataggaca cactacgcagactccgtgaagggccgcttcaccatctccagagacaactccaagaacacgctgtatttgcaaatgtacagcctgagagtcgaggacacggccttatattactgt atgaacgaactcggatattgtagtggtggg IGA1 23 213 15 8 11 17 4 6 6 2 6 9 JY8QFUQ01CXD17 16050 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 828 87.1 189/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-25*01 F 1 8 8 12 8.8.12 [1.17.38.11] CVRGTREWSGIDYW in-frame - ctctggattcattttcagtaactactggatgcactgggtccgccaagctccagggaaggggctgctctgggtctcagtcattaatagtgacggcagtgaaattcactacgcggactccgtgaggggccgattcaccatctcccgagacaacgccaagaacacgctgtatttgcaaatggacaatctcagaggcgacgacacggctctatattattgtgtaagagggacaagggaatggagcgggattgactactggggtcagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [284] c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g107>a,S36>N(- - -)|g151>c,V51>L(+ - +)|g153>c,V51>L(+ - +)|c163>g,R55>V(- - -)|g164>t,R55>V(- - -)|t165>c,R55>V(- - -)|t177>c|g186>c|a190>g,S64>E(- - -)|g191>a,S64>E(- - -)|c192>a,S64>E(- - -)|c194>t,T65>I(- - -)|a195>t,T65>I(- - -)|a196>c,S66>H(+ - -)|g197>a,S66>H(+ - -)|a215>g,K72>R(+ + +)|a238>c|c265>t|a274>g,N92>D(+ + -)|g278>a,S93>N(- - -)|g282>c|c287>g,A96>G(- + -)|g291>c,E97>D(+ - +)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)|c309>t|c314>t,A105>V(+ - +)| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g107>a,S36>N(- - -)| g151>c,V51>L(+ - +)|g153>c,V51>L(+ - +)|c163>g,R55>V(- - -)|g164>t,R55>V(- - -)|t165>c,R55>V(- - -)| t177>c|g186>c|a190>g,S64>E(- - -)|g191>a,S64>E(- - -)|c192>a,S64>E(- - -)|c194>t,T65>I(- - -)|a195>t,T65>I(- - -)| a196>c,S66>H(+ - -)|g197>a,S66>H(+ - -)|a215>g,K72>R(+ + +)|a238>c|c265>t|a274>g,N92>D(+ + -)|g278>a,S93>N(- - -)|g282>c|c287>g,A96>G(- + -)|g291>c,E97>D(+ - +)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)|c309>t| c314>t,A105>V(+ - +)|a320>g| 319 (320) 224 (225) 195 29 (30) 6 (7) 23 3 (4) 5 6 2 2 1 1 0 6 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 2 0 0 0 0 0 0 0 0 0 51 51 46 5 0 5 0 0 0 1 0 0 0 0 2 1 1 0 30 24 17 7 2 5 1 1 1 1 0 1 1 0 1 0 0 0 117 114 101 13 4 9 2 3 2 0 2 0 0 0 3 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcattttcagtaactactgg atgcactgggtccgccaagctccagggaaggggctgctctgggtctcagtc attaatagtgacggcagtgaaatt cactacgcggactccgtgaggggccgattcaccatctcccgagacaacgccaagaacacgctgtatttgcaaatggacaatctcagaggcgacgacacggctctatattattgt gtaagagggacaagggaatggagcgggattgactac VRGTREWSGIDY IGHV3-74 IGHD6-25 IGHJ4 ggattcattttcagtaactactgg atgcactgggtccgccaagctccagggaaggggctgctctgggtctcagtc attaatagtgacggcagtgaaatt cactacgcggactccgtgaggggccgattcaccatctcccgagacaacgccaagaacacgctgtatttgcaaatggacaatctcagaggcgacgacacggctctatattattgt gtaagagggacaagggaatggagcgggattgactac IGA1 28 213 15 13 10 20 5 8 4 2 14 8 JY8QFUQ01CXIGS 6740 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*04 F or Homsap IGHV3-33*06 F 815 86.24 188/218 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARPHHNSGYSFDHW in-frame + cgtctgatttcaacgtcggtgactttgacatgcactgggtccgccagactccagacaaggggctggagtgggtggcacttttttggtatgacggaaagaggaaatattatgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacaccctgtatctgcaaatggacagcctgagagccgacgacacggctacctacttttgtgcgaggcctcatcataatagtggttattcctttgaccactggggccagggaacccaggtcaccgtctcctctgcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 89 [288, 287] g80>a,G27>D(- - -)|a81>t,G27>D(- - -)|c86>a,T29>N(- + -)|t88>g,F30>V(+ - -)|a103>g,S35>G(+ + -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>t,Y37>F(- + -)|g113>a,G38>D(- - -)|g133>a,A45>T(- - -)|g140>a,G47>D(- - -)|g163>c,V55>L(+ - +)|a166>t,I56>F(+ - -)|a168>t,I56>F(+ - -)|t177>c|g188>a,S63>K(- - -)|t189>g,S63>K(- - -)|a191>g,N64>R(+ - -)|t192>g,N64>R(+ - -)|c198>t|a225>g|g258>c|a274>g,N92>D(+ + -)|g291>c,E97>D(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>c,V101>T(- - -)|t306>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g80>a,G27>D(- - -)|a81>t,G27>D(- - -)|c86>a,T29>N(- + -)|t88>g,F30>V(+ - -)|a103>g,S35>G(+ + -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a110>t,Y37>F(- + -)|g113>a,G38>D(- - -)| g133>a,A45>T(- - -)|g140>a,G47>D(- - -)|g163>c,V55>L(+ - +)| a166>t,I56>F(+ - -)|a168>t,I56>F(+ - -)|t177>c|g188>a,S63>K(- - -)|t189>g,S63>K(- - -)|a191>g,N64>R(+ - -)|t192>g,N64>R(+ - -)| c198>t|a225>g|g258>c|a274>g,N92>D(+ + -)|g291>c,E97>D(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>c,V101>T(- - -)|t306>c|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| a318>g|g319>c|a320>c| 317 (320) 223 (226) 193 30 (33) 5 (8) 25 5 (6) 7 2 3 0 (1) 1 5 0 4 (5) 0 0 3 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 15 9 0 9 2 3 0 0 0 1 2 0 0 0 0 1 51 51 48 3 0 3 0 2 0 0 0 0 0 0 1 0 0 0 30 24 17 7 1 6 1 1 0 1 0 0 2 0 0 0 0 2 117 114 103 11 4 7 2 1 2 2 0 0 1 0 3 0 0 0 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cgtct gatttcaacgtcggtgactttgac atgcactgggtccgccagactccagacaaggggctggagtgggtggcactt ttttggtatgacggaaagaggaaa tattatgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacaccctgtatctgcaaatggacagcctgagagccgacgacacggctacctacttttgt gcgaggcctcatcataatagtggttattcctttgaccac ARPHHNSGYSFDH IGHV3-33 IGHD3-22 IGHJ4 gatttcaacgtcggtgactttgac atgcactgggtccgccagactccagacaaggggctggagtgggtggcactt ttttggtatgacggaaagaggaaa tattatgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacaccctgtatctgcaaatggacagcctgagagccgacgacacggctacctacttttgt gcgaggcctcatcataatagtggttattcctttgaccac IGA2 30 213 17 13 9 14 8 16 4 1 10 15 JY8QFUQ01CXM4M 16324 productive (see comment) Homsap IGHV6-1*01 F, or Homsap IGHV6-1*02 F 1003 94.22 212/225 nt 93.78 211/225 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD1-14*01 ORF 3 10 9 19 10.9.19 [1.16.38.11] CARASRRDRQSDRNYYELDFW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 62 in the user submitted sequence), (cause frameshift) ctccggggacagtgtctctagcaacagtgccacttggaactggatcaggcagtccccaacggaggccttgagtggctgggaaggacatcctacaggtccaaatggtatagtgattatgcggtgtctgtgaaaagtcgaataaccatcaacccagacacatccaagaaccagttctccctgcaattgaactccgttagtcccgaggacacggctgtgtattactgtgcaagagcttccagacgcgatcgtcagtctgatcggaactactatgagctggacttctggggccagggaatcttggtcgccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [313] t111>c|g112>a,A38>T(- - -)|t139>a,S47>T(+ - +)|a170>c,Y57>S(+ - -)|g186>a|a194>g,N65>S(- - -)|a204>g|a207>g|g270>a|c271>t|t279>c|g282>t|c284>g,T95>S(+ - +)| t111>c|g112>a,A38>T(- - -)| t139>a,S47>T(+ - +)| a170>c,Y57>S(+ - -)|g186>a|a194>g,N65>S(- - -)| a204>g|a207>g|g270>a|c271>t|t279>c|g282>t|c284>g,T95>S(+ - +)| a320>c| 319 (320) 232 (233) 219 13 (14) 8 (9) 5 3 3 1 2 1 (2) 0 0 1 0 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 1 1 0 1 0 1 0 0 0 0 0 0 0 0 51 50 49 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 30 27 24 3 1 2 1 1 0 0 1 0 0 0 0 0 0 0 117 114 107 7 6 1 2 1 1 1 0 0 0 0 0 1 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,5-6(FR1)|aa,40-41(FR1)|aa,103-104(CDR1)|aa,118-119(FR2)|aa,162-163(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,184-185(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|ta,206-207(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,227-228(FR3)|aa,235-236(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,5-6(FR1)|tt,114-115(CDR1)|tt,149-150(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|tt,198-199(FR3)|ta,206-207(FR3)|ta,227-228(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggta,3-6(FR1)|agct,8-11(FR1)|agca,14-17(FR1)|agca,100-103(CDR1)|ggca,128-131(FR2)|ggct,155-158(FR2)|ggta,188-191(CDR2)|agta,204-207(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tgcc,69-72(FR1)|tgct,108-111(CDR1)|aact,118-121(FR2)|tact,169-172(CDR2)|aacc,228-231(FR3)|aacc,235-238(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|aact,274-277(FR3)|tact,307-310(FR3)| ctcc ggggacagtgtctctagcaacagtgccact tggaactggatcaggcagtccccaacggaggccttgagtggctgggaagg acatcctacaggtccaaatggtatagt gattatgcggtgtctgtgaaaagtcgaataaccatcaacccagacacatccaagaaccagttctccctgcaattgaactccgttagtcccgaggacacggctgtgtattactgt gcaagagcttccagacgcgatcgtcagtctgatcggaactactatgagctggacttc ARASRRDRQSDRNYYELDF IGHV6-1 IGHD1-14 IGHJ4 ggggacagtgtctctagcaacagtgccact tggaactggatcaggcagtccccaacggaggccttgagtggctgggaagg acatcctacaggtccaaatggtatagt gattatgcggtgtctgtgaaaagtcgaataaccatcaacccagacacatccaagaaccagttctccctgcaattgaactccgttagtcccgaggacacggctgtgtattactgt gcaagagcttccagacgcgatcgtcagtctgatcggaactactatgagctggacttc IGA2 13 221 9 4 4 6 5 7 6 2 2 3 JY8QFUQ01CY2HZ 13288 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 1035 97.7 212/217 nt NA Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD2-21*02 F 2 8 8 17 8.8.17 [1.17.38.11] CARPYCGGDCHNRGAFDIW in-frame + gtctggattcaccttcagtagctatggcatacactgggtccgccaggctccaggcaaggggctggagtgggtggcagttatatggtatgatggaagtgaaatatactatgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgaggccatattgtggtggtgactgccacaatcgaggggcttttgatatctggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [299] g117>a,M39>I(+ + -)|a190>g,N64>E(+ - -)|t192>a,N64>E(+ - -)|a194>t,K65>I(- + -)|a225>c| g117>a,M39>I(+ + -)| a190>g,N64>E(+ - -)|t192>a,N64>E(+ - -)|a194>t,K65>I(- + -)| a225>c| a318>g|g319>c|a320>c| 317 (320) 222 (225) 217 5 (8) 1 (4) 4 1 (2) 1 0 0 1 (2) 0 1 1 0 (1) 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 1 1 0 0 0 0 117 114 113 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcaccttcagtagctatggc atacactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatggtatgatggaagtgaaata tactatgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgaggccatattgtggtggtgactgccacaatcgaggggcttttgatatc ARPYCGGDCHNRGAFDI IGHV3-33 IGHD2-21 IGHJ3 ggattcaccttcagtagctatggc atacactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatggtatgatggaagtgaaata tactatgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgaggccatattgtggtggtgactgccacaatcgaggggcttttgatatc IGA1 5 213 2 3 1 1 1 4 1 0 1 3 JY8QFUQ01CY3ZT 12889 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 877 89.81 194/216 nt 89.35 193/216 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-10*01 F 3 8 8 10 8.8.10 [1.16.38.11] CSKDTFGREDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 2 nucleotides (from position 58 in the user submitted sequence), (cause frameshift) cctctggattctccgtcagtaattactggatgcactgggtccgccaggctccagggagggctggagtgggtctcacgtattaatgaagatggtagtcggacagactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgtagtaaagacacatttgggcgagaagactactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccgcaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 71 95 [277, 278] a85>t,T29>S(+ - +)|t88>g,F30>V(+ - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a132>g|t152>a,V51>E(- + -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>t|a190>c,S64>R(- - -)|c192>g,S64>R(- - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c234>t|c236>a,S79>Y(+ - -)|a245>g,N82>S(- - -)|g258>a|c287>t,A96>V(+ - +)|g291>a|g303>c|c309>t| a85>t,T29>S(+ - +)|t88>g,F30>V(+ - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a132>g|t152>a,V51>E(- + -)| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>t|a190>c,S64>R(- - -)|c192>g,S64>R(- - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c234>t|c236>a,S79>Y(+ - -)|a245>g,N82>S(- - -)|g258>a|c287>t,A96>V(+ - +)|g291>a|g303>c|c309>t| g313>a,A105>S(- + -)|c314>g,A105>S(- + -)|a315>t,A105>S(- + -)|g317>a,R106>K(+ + +)| 320 224 198 26 7 19 4 7 4 0 1 1 2 2 1 2 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 1 0 0 0 1 0 0 0 0 1 51 49 47 2 1 1 1 0 0 0 0 0 0 1 0 0 0 0 30 24 18 6 1 5 1 1 0 0 1 0 0 1 0 1 1 0 117 114 104 10 5 5 2 3 3 0 0 1 0 0 1 0 0 0 8 8 4 4 0 4 0 2 0 0 0 0 1 0 0 1 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattctccgtcagtaattactgg atgcactgggtccgccaggctccagggagggctggagtgggtctcacgt attaatgaagatggtagtcggaca gactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgt agtaaagacacatttgggcgagaagactac SKDTFGREDY IGHV3-74 IGHD3-10 IGHJ4 ggattctccgtcagtaattactgg atgcactgggtccgccaggctccagggagggctggagtgggtctcacgt attaatgaagatggtagtcggaca gactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgt agtaaagacacatttgggcgagaagactac IGA2 22 211 13 9 9 13 4 9 6 1 6 9 JY8QFUQ01CYT8I 1145 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 990 95.39 207/217 nt NA Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 133 74 37/50 nt Homsap IGHD2-15*01 F 3 8 8 17 8.8.17 [1.17.38.11] CARDTLEVVSNMDDAFDFW in-frame + ctctggattcaccttcagtcgttatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatacattagtaggactactactgacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatatttctgtgcgagagatactctagaggtggtgtctaatatggatgatgcttttgatttctggggccaagggacagtggtaatgtctcctagcatcccgaccagcccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccc NA IGA2 79 84 [294, 295] a106>c,S36>R(- - -)|c108>t,S36>R(- - -)|c164>a,S55>Y(+ - -)|t174>g,S58>R(- - -)|g176>c,S59>T(+ - +)|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t190>g,Y64>D(- - -)|g303>a|a308>t,Y103>F(- + -)| a106>c,S36>R(- - -)|c108>t,S36>R(- - -)| c164>a,S55>Y(+ - -)| t174>g,S58>R(- - -)|g176>c,S59>T(+ - +)|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t190>g,Y64>D(- - -)| g303>a|a308>t,Y103>F(- + -)| 320 225 215 10 1 9 0 1 1 0 1 1 1 0 3 0 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 1 0 1 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 30 24 19 5 0 5 0 0 0 0 0 0 0 0 3 0 0 2 117 114 112 2 1 1 0 1 0 0 0 0 1 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtcgttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatac attagtaggactactactgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatatttctgt gcgagagatactctagaggtggtgtctaatatggatgatgcttttgatttc ARDTLEVVSNMDDAFDF IGHV3-21 IGHD2-15 IGHJ3 ggattcaccttcagtcgttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatac attagtaggactactactgacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatatttctgt gcgagagatactctagaggtggtgtctaatatggatgatgcttttgatttc IGA2 10 213 2 8 2 6 0 4 1 0 2 7 JY8QFUQ01CYTAI 12508 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F (see comment) 705 81.31 174/214 nt 79.91 171/214 nt Homsap IGHJ2*01 F 238 94.34 50/53 nt Homsap IGHD6-6*01 F 3 8 7 9 8.7.9 [1.17.38.11] CARLDGHFDLW in-frame - Potentially, because of detected insertion/deletion Low V-REGION identity (81,31% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR2-IMGT at codon 41 of V-REGION: 'C' (1 nucleotides at position 35 to 35 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 68 of V-REGION: 'G' (1 nucleotides at position 109 to 109 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 72 of V-REGION: 'G' (1 nucleotides at position 122 to 122 in the user submitted sequence), (cause frameshift); ctctgggttcaccatcagtcactactccatgggcCtgggtccgccaggctccgggaaaggggctggagtgggtctcattaatttatcccggcgggagcacattccacgGcagactccgtgaGgggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgtgcgagactcgacgggcacttcgatctctggggccgtggcaccctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggagctcag NA IGG1 100 100 [276] g88>a,V30>I(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)|a118>g,S40>G(+ + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g|a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c222>g|a225>g|t226>c,F76>L(+ - -)|c228>t,F76>L(+ - -)|c230>t,T77>I(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| g88>a,V30>I(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a109>t,N37>Y(- - -)|a113>c,Y38>S(+ - -)| a118>g,S40>G(+ + -)|a138>g|g141>a|g163>t,V55>L(+ - +)|t165>a,V55>L(+ - +)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|t177>c|t189>g| a197>t,Y66>F(- + -)|t199>c,Y67>H(+ - -)|a215>g,K72>R(+ + +)|c222>g|a225>g|t226>c,F76>L(+ - -)|c228>t,F76>L(+ - -)|c230>t,T77>I(- - -)|a244>g,N82>E(+ - -)|t246>a,N82>E(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a256>g,T86>E(- - -)|c257>a,T86>E(- - -)|g258>a,T86>E(- - -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>g,N92>K(+ - -)|g278>a,S93>K(- - -)|c279>a,S93>K(- - -)|c280>g,L94>V(+ - +)|g284>a,R95>K(+ + +)|c287>t,A96>V(+ - +)|g291>a|g303>a|c309>t| g319>c|a320>t| 318 (320) 220 (222) 180 40 (42) 9 (11) 31 9 8 5 3 3 2 2 (3) 2 1 (2) 3 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 2 0 0 2 0 1 0 0 0 0 0 51 51 46 5 2 3 2 1 0 0 0 0 0 1 0 0 1 0 30 21 17 4 2 2 0 0 0 1 1 0 0 0 1 0 0 1 117 114 88 26 5 21 7 5 5 2 0 2 1 1 0 3 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcaccatcagtcactactcc atgggctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgagggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc ARLDGHFDL IGHV3-53 IGHD6-6 IGHJ2 gggttcaccatcagtcactactcc atgggctgggtccgccaggctccgggaaaggggctggagtgggtctcatta atttatcccggcgggagcaca ttccacgcagactccgtgagggggcggcttatcatctccagagacgaatccaagagtgaactgtatcttcggatgaagaaagtgaaagtcgaagacacggccgtatattattgt gcgagactcgacgggcacttcgatctc IGG1 40 210 25 15 13 20 12 20 7 2 24 7 JY8QFUQ01CYU3K 8881 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 963 94.01 204/217 nt NA Homsap IGHJ6*02 F 265 91.94 57/62 nt Homsap IGHD1-20*01 F 1 8 8 19 8.8.19 [1.17.38.11] CANASPRITGTYFYYYGMDVW in-frame + ctctggattcatcttcagtagctatgccatgaattgggtccgccagactccagggaaggggctggagtgggtctcagccattagtggtagtggtggtaacacatactacgcagactccgtgaagggccggttcaccgtctccagagacaattccaacaacacgctgtatctgcaattggacagcctgcgagccgaggacacggccgtatattactgtgcgaatgcctccccccgtataactggaacgtatttctactactacggtatggacgtctggggccaagggaccacggtcagcgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA IGG3 93 100 [306] c86>t,T29>I(- - -)|t90>c|c105>t|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g133>a,A45>T(- - -)|t165>c|g191>a,S64>N(- - -)|a232>g,I78>V(+ - +)|g252>c,K84>N(+ - -)|a271>t,M91>L(+ + -)|a274>g,N92>D(+ + -)|a283>c| c86>t,T29>I(- - -)|t90>c|c105>t| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|g133>a,A45>T(- - -)|t165>c| g191>a,S64>N(- - -)| a232>g,I78>V(+ - +)|g252>c,K84>N(+ - -)|a271>t,M91>L(+ + -)|a274>g,N92>D(+ + -)|a283>c| a318>t,K106>N(+ - -)|a320>c| 317 (320) 222 (225) 209 (210) 13 (15) 4 (5) 9 (10) 2 3 3 2 1 (2) 0 1 (2) 0 1 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 2 1 0 0 0 0 0 0 0 0 51 51 47 4 1 3 0 2 1 1 0 0 0 0 0 0 0 0 30 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 117 114 109 5 1 4 2 0 0 0 1 0 1 0 1 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagtagctatgcc atgaattgggtccgccagactccagggaaggggctggagtgggtctcagcc attagtggtagtggtggtaacaca tactacgcagactccgtgaagggccggttcaccgtctccagagacaattccaacaacacgctgtatctgcaattggacagcctgcgagccgaggacacggccgtatattactgt gcgaatgcctccccccgtataactggaacgtatttctactactacggtatggacgtc ANASPRITGTYFYYYGMDV IGHV3-23 IGHD1-20 IGHJ6 ggattcatcttcagtagctatgcc atgaattgggtccgccagactccagggaaggggctggagtgggtctcagcc attagtggtagtggtggtaacaca tactacgcagactccgtgaagggccggttcaccgtctccagagacaattccaacaacacgctgtatctgcaattggacagcctgcgagccgaggacacggccgtatattactgt gcgaatgcctccccccgtataactggaacgtatttctactactacggtatggacgtc IGG3 13 213 10 3 6 7 4 6 2 2 7 2 JY8QFUQ01CYX7E 16385 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 875 88.69 196/221 nt 87.78 194/221 nt Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD6-6*01 F 2 10 7 16 10.7.16 [1.17.38.11] CTNDRSMAVRATHAFHIW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 39 of V-REGION: 'G' (1 nucleotides at position 37 to 37 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 79 of V-REGION: 'T' (1 nucleotides at position 145 to 145 in the user submitted sequence), (cause frameshift); tctctggtgactccattggtagcagtgcctactactGggggctgggtccgccagccccccgggaaggggctggagtggattggaagtatctattatggtggcaacacctactacaacccgtccctcaggagtcgagtcagcattTtccgcagacacgtccaagaaccagttctccctgcatctctactccgtgaccgccgcagacacggctctgtattactgtacgaatgaccgttcgatggccgttcgtgcaactcatgcttttcatatctggggcctagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [302, 301] g83>a,G28>D(- - -)|c90>t|a91>g,S31>G(+ + -)|c93>t,S31>G(+ + -)|t102>c|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|t108>c,S36>A(- + -)|a124>g,I42>V(+ - +)|a138>c|g162>a|a175>g,S59>G(+ + -)|g189>c|g191>a,S64>N(- - -)|a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|a234>t|t239>c,V80>A(+ - +)|a268>c,K90>H(- - +)|g270>t,K90>H(- - +)|g273>c|a274>t,S92>Y(+ - -)|g275>a,S92>Y(+ - -)|t279>c|g301>c,V101>L(+ - +)|g313>a,A105>T(- - -)| g83>a,G28>D(- - -)|c90>t|a91>g,S31>G(+ + -)|c93>t,S31>G(+ + -)|t102>c|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|t108>c,S36>A(- + -)| a124>g,I42>V(+ - +)|a138>c|g162>a| a175>g,S59>G(+ + -)|g189>c|g191>a,S64>N(- - -)| a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|a234>t|t239>c,V80>A(+ - +)|a268>c,K90>H(- - +)|g270>t,K90>H(- - +)|g273>c|a274>t,S92>Y(+ - -)|g275>a,S92>Y(+ - -)|t279>c|g301>c,V101>L(+ - +)| g313>a,A105>T(- - -)|g317>a,R106>N(+ - -)|a318>t,R106>N(+ - -)|c319>g| 316 (320) 225 (229) 199 (200) 26 (29) 8 (9) 18 (20) 5 5 (6) 2 4 2 0 2 (3) 0 4 1 (2) 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 22 8 2 6 2 1 2 2 0 0 0 0 1 0 0 0 51 51 48 3 2 1 1 1 0 0 1 0 0 0 0 0 0 0 30 21 18 3 1 2 1 1 0 0 0 0 0 0 1 0 0 0 117 114 103 11 3 8 1 1 0 2 1 0 2 0 2 1 1 0 4 (8) 4 3 (4) 1 (4) 0 (1) 1 (3) 0 1 (2) 0 0 0 0 0 (1) 0 0 0 (1) 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgactccattggtagcagtgcctactac tggggctgggtccgccagccccccgggaaggggctggagtggattggaagt atctattatggtggcaacacc tactacaacccgtccctcaggagtcgagtcagcatttccgcagacacgtccaagaaccagttctccctgcatctctactccgtgaccgccgcagacacggctctgtattactgt acgaatgaccgttcgatggccgttcgtgcaactcatgcttttcatatc TNDRSMAVRATHAFHI IGHV4-39 IGHD6-6 IGHJ3 ggtgactccattggtagcagtgcctactac tggggctgggtccgccagccccccgggaaggggctggagtggattggaagt atctattatggtggcaacacc tactacaacccgtccctcaggagtcgagtcagcatttccgcagacacgtccaagaaccagttctccctgcatctctactccgtgaccgccgcagacacggctctgtattactgt acgaatgaccgttcgatggccgttcgtgcaactcatgcttttcatatc IGA2 25 216 15 10 6 12 9 13 5 3 9 8 JY8QFUQ01CZ1IL 18012 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 927 92.17 200/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-16*02 F 1 8 8 14 8.8.14 [1.17.38.11] CAKVGLMSARDRIDYW in-frame + ctctggattcacctttagcaactatgccatgagttgggtccgccaggctcaagggaaggggctggactgggtctcagatattagtaatagtggtggtgacacattctacgcaggctccgtgaagggccgcttcaccatctccagagacaattccaggaacactctatatctgcaaatggacagcctgagagccgaggacacggccgtgtattactgtgcgaaagtggggctgatgtccgctcgagaccgcattgactactggggccggggaaccctggtctccgtctcctcaccatccccgaccagccccaaggtcttcccgctgagcctctgcagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 71 100 [290] g107>a,S36>N(- - -)|c120>t|c137>a,P46>Q(- - -)|g153>c,E51>D(+ - +)|c164>a,A55>D(- - -)|g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a197>t,Y66>F(- + -)|a206>g,D69>G(- - -)|g225>c|a251>g,K84>R(+ + +)|g258>t|g261>a|a274>g,N92>D(+ + -)|a303>g| g107>a,S36>N(- - -)| c120>t|c137>a,P46>Q(- - -)|g153>c,E51>D(+ - +)|c164>a,A55>D(- - -)| g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a197>t,Y66>F(- + -)|a206>g,D69>G(- - -)|g225>c|a251>g,K84>R(+ + +)|g258>t|g261>a|a274>g,N92>D(+ + -)|a303>g| a320>t| 319 (320) 224 (225) 207 17 (18) 5 (6) 12 5 5 1 0 0 2 1 (2) 0 2 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 47 4 1 3 0 0 1 0 0 2 0 0 1 0 0 0 30 24 20 4 0 4 1 3 0 0 0 0 0 0 0 0 0 0 117 114 106 8 4 4 4 1 0 0 0 0 1 0 1 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcaactatgcc atgagttgggtccgccaggctcaagggaaggggctggactgggtctcagat attagtaatagtggtggtgacaca ttctacgcaggctccgtgaagggccgcttcaccatctccagagacaattccaggaacactctatatctgcaaatggacagcctgagagccgaggacacggccgtgtattactgt gcgaaagtggggctgatgtccgctcgagaccgcattgactac AKVGLMSARDRIDY IGHV3-23 IGHD3-16 IGHJ4 ggattcacctttagcaactatgcc atgagttgggtccgccaggctcaagggaaggggctggactgggtctcagat attagtaatagtggtggtgacaca ttctacgcaggctccgtgaagggccgcttcaccatctccagagacaattccaggaacactctatatctgcaaatggacagcctgagagccgaggacacggccgtgtattactgt gcgaaagtggggctgatgtccgctcgagaccgcattgactac IGA2 17 213 11 6 6 11 5 6 5 0 7 5 JY8QFUQ01CZDE0 13373 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 815 86.24 188/218 nt NA Homsap IGHJ5*02 F 228 94.12 48/51 nt Homsap IGHD2-21*01 F 3 8 8 16 8.8.16 [1.17.38.11] CAKDPQQILAEVNWFDPW in-frame + cctctggattcacctttcgcagctacgccatgagttgggtccgccaggctccaggaagggggctggagtgggtctcctcaatcagtggcagtggtgataaaacaaaatatgcagactccgtgaagggccgattcaccatctccagagacaatgcgaggaacactttttatctgcatatgaacagcctcagagccgcggacacgggcgtctactattgtgcgaaagatcctcagcagatactggcagaagtaaactggttcgacccctggggccagggaacccaggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] a103>c,S35>R(- - -)|t111>c|c120>t|g141>a|a143>g,K48>R(+ + +)|a162>c|g163>t,A55>S(- + -)|t165>a,A55>S(- + -)|t168>c|t174>c|g188>a,G63>D(- - -)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|t196>a,Y66>K(- - -)|c198>a,Y66>K(- - -)|c201>t|g225>a|t247>g,S83>A(- + -)|c249>g,S83>A(- + -)|a251>g,K84>R(+ + +)|g258>t|c259>t,L87>F(+ - -)|g261>t,L87>F(+ - -)|a270>t,Q90>H(- + -)|g282>c|a290>c,E97>A(- - -)|c299>g,A100>G(- + -)|a303>c|t306>c|c309>t| a103>c,S35>R(- - -)|t111>c| c120>t|g141>a|a143>g,K48>R(+ + +)|a162>c|g163>t,A55>S(- + -)|t165>a,A55>S(- + -)| t168>c|t174>c|g188>a,G63>D(- - -)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)| t196>a,Y66>K(- - -)|c198>a,Y66>K(- - -)|c201>t|g225>a|t247>g,S83>A(- + -)|c249>g,S83>A(- + -)|a251>g,K84>R(+ + +)|g258>t|c259>t,L87>F(+ - -)|g261>t,L87>F(+ - -)|a270>t,Q90>H(- + -)|g282>c|a290>c,E97>A(- - -)|c299>g,A100>G(- + -)|a303>c|t306>c|c309>t| 320 226 196 30 13 17 2 4 4 4 4 2 1 2 1 2 3 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 0 1 1 0 0 0 0 0 0 0 51 51 45 6 3 3 1 1 1 0 1 0 0 1 0 0 1 0 30 24 19 5 2 3 0 2 0 2 0 1 0 0 0 0 0 0 117 114 97 17 7 10 1 1 3 1 2 1 1 1 1 2 2 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttcgcagctacgcc atgagttgggtccgccaggctccaggaagggggctggagtgggtctcctca atcagtggcagtggtgataaaaca aaatatgcagactccgtgaagggccgattcaccatctccagagacaatgcgaggaacactttttatctgcatatgaacagcctcagagccgcggacacgggcgtctactattgt gcgaaagatcctcagcagatactggcagaagtaaactggttcgacccc AKDPQQILAEVNWFDP IGHV3-23 IGHD2-21 IGHJ5 ggattcacctttcgcagctacgcc atgagttgggtccgccaggctccaggaagggggctggagtgggtctcctca atcagtggcagtggtgataaaaca aaatatgcagactccgtgaagggccgattcaccatctccagagacaatgcgaggaacactttttatctgcatatgaacagcctcagagccgcggacacgggcgtctactattgt gcgaaagatcctcagcagatactggcagaagtaaactggttcgacccc IGA1 30 213 14 16 8 16 6 14 10 3 13 4 JY8QFUQ01CZH0L 6549 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 931 92.59 200/216 nt 91.67 198/216 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-10*01 F 2 8 8 13 8.8.13 [1.16.38.11] CANWLSGTHSKFDYW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 59 of V-REGION: 'A' (1 nucleotides at position 89 to 89 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 58 in the user submitted sequence), (cause frameshift) ctctggattcaccttcagcaagtatgccatgagctgggtccgccaggctccaggggagggctgcagtgggtctcagcaattagtggaaAatggtgctgatatatactacgcagactccgtgaacggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgtgcgaattggctttcggggacacattctaagtttgactactggggcaagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [287, 286] t90>c|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a142>g|g151>c,E51>Q(+ + -)|t165>a|t174>a|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c192>t,S64>D(- - -)|c194>t,T65>I(- - -)|g216>c,K72>N(+ - -)|g261>a|t306>c| t90>c|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)| a142>g|g151>c,E51>Q(+ + -)|t165>a| t174>a|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c192>t,S64>D(- - -)|c194>t,T65>I(- - -)| g216>c,K72>N(+ - -)|g261>a|t306>c| a318>t,K106>N(+ - -)|g319>t|a320>g| 317 (320) 221 (224) 205 16 (19) 6 (8) 10 (11) 2 (3) 4 2 2 0 0 0 (1) 2 3 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 0 1 0 0 0 0 0 1 0 0 51 50 47 3 2 1 1 0 0 0 0 0 0 1 1 0 0 0 30 24 17 7 1 6 1 2 2 0 0 0 0 1 1 0 0 0 117 114 111 3 2 1 0 1 0 1 0 0 0 0 1 0 0 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagcaagtatgcc atgagctgggtccgccaggctccaggggagggctgcagtgggtctcagca attagtggaaatggtgctgatata tactacgcagactccgtgaacggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgt gcgaattggctttcggggacacattctaagtttgactac ANWLSGTHSKFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcaccttcagcaagtatgcc atgagctgggtccgccaggctccaggggagggctgcagtgggtctcagca attagtggaaatggtgctgatata tactacgcagactccgtgaacggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgt gcgaattggctttcggggacacattctaagtttgactac IGA2 16 212 10 6 6 10 4 6 4 2 2 8 JY8QFUQ01DABGE 6896 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD4-23*01 ORF 2 8 8 11 8.8.11 [1.17.38.11] CNLHPYGDSSDYW in-frame + ctctggactcaccttcagtcgcctctggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtatagatagtgatgggaataacataatctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaaaacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtatattactgtaaccttcatccctacggtgactcctccgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [282] t82>c,F28>L(+ - -)|a106>c,S36>R(- - -)|t109>c,Y37>L(- - -)|a110>t,Y37>L(- - -)|t168>a|a169>g,N57>D(+ + -)|g188>a,S63>N(- - -)|g191>a,S64>N(- - -)|c194>t,T65>I(- - -)|g197>t,S66>I(- - -)|c255>a,N85>K(+ - -)|g303>a| t82>c,F28>L(+ - -)|a106>c,S36>R(- - -)|t109>c,Y37>L(- - -)|a110>t,Y37>L(- - -)| t168>a|a169>g,N57>D(+ + -)|g188>a,S63>N(- - -)|g191>a,S64>N(- - -)|c194>t,T65>I(- - -)| g197>t,S66>I(- - -)|c255>a,N85>K(+ - -)|g303>a| g313>a,A105>N(- - -)|c314>a,A105>N(- - -)|a315>c,A105>N(- - -)|a316>c,R106>L(- + -)|g317>t,R106>L(- + -)|a318>t,R106>L(- + -)|g319>c| 312 (320) 217 (225) 205 (206) 12 (19) 2 (3) 10 (16) 1 3 (4) 1 2 1 (3) 1 (2) 1 (2) 1 0 (1) 0 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 2 1 0 1 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 19 5 1 4 1 2 1 0 0 0 0 1 0 0 0 0 117 114 111 3 1 2 0 1 0 0 0 1 0 0 0 0 1 0 0 (8) 0 0 (1) 0 (7) 0 (1) 0 (6) 0 0 (1) 0 0 0 (2) 0 (1) 0 (1) 0 0 (1) 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggactcaccttcagtcgcctctgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt atagatagtgatgggaataacata atctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaaaacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtatattactgt aaccttcatccctacggtgactcctccgactac NLHPYGDSSDY IGHV3-74 IGHD4-23 IGHJ4 ggactcaccttcagtcgcctctgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt atagatagtgatgggaataacata atctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaaaacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtatattactgt aaccttcatccctacggtgactcctccgactac IGG2 12 213 7 5 4 6 3 6 1 1 2 8 JY8QFUQ01DARQJ 8850 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 945 93.09 202/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD4-23*01 ORF 2 8 8 13 8.8.13 [1.17.38.11] CARGGGGKSIPLDNW in-frame + gtctggcttcacgttcagtagttatggcatgcaatgggtccgccaggctccaggcaaggggctggagtgggtggcagttatttggtatgatggaagtaataaatattatgcagactccgtgaagggccgattcgacatctccagagacaattccaagaacacactatatctgcaaatgaacaacctgagagccgaggacacggctatgtaccattgtgcgagaggcggcggtggtaagtccattccacttgacaactggggccagggaaccctggtcaccgtctcctcagcatccccggaccagccccaaggtcttcccgctgagcctcgacagcaccccccctaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccag NA IGA2 71 89 [288, 290] a81>c|c87>g|c108>t|c120>a,H40>Q(- + -)|a168>t|c198>t|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|g258>a|g261>a|g278>a,S93>N(- - -)|g301>a,V101>M(+ - -)|t306>c|t307>c,Y103>H(+ - -)|c309>t,Y103>H(+ - -)| a81>c|c87>g|c108>t| c120>a,H40>Q(- + -)| a168>t| c198>t|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|g258>a|g261>a|g278>a,S93>N(- - -)|g301>a,V101>M(+ - -)|t306>c|t307>c,Y103>H(+ - -)|c309>t,Y103>H(+ - -)| a320>g| 319 (320) 224 (225) 209 15 (16) 8 (9) 7 1 (2) 4 3 2 1 2 1 0 0 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 3 0 0 0 1 0 1 0 0 0 0 1 0 0 51 51 50 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 30 24 23 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 117 114 104 10 4 6 1 4 2 2 0 1 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggcttcacgttcagtagttatggc atgcaatgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atttggtatgatggaagtaataaa tattatgcagactccgtgaagggccgattcgacatctccagagacaattccaagaacacactatatctgcaaatgaacaacctgagagccgaggacacggctatgtaccattgt gcgagaggcggcggtggtaagtccattccacttgacaac ARGGGGKSIPLDN IGHV3-33 IGHD4-23 IGHJ4 ggcttcacgttcagtagttatggc atgcaatgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atttggtatgatggaagtaataaa tattatgcagactccgtgaagggccgattcgacatctccagagacaattccaagaacacactatatctgcaaatgaacaacctgagagccgaggacacggctatgtaccattgt gcgagaggcggcggtggtaagtccattccacttgacaac IGA2 15 213 10 5 7 10 3 5 4 4 7 0 JY8QFUQ01DDQ8A 10866 productive (see comment) Homsap IGHV3-7*02 F 960 93.18 205/220 nt 92.27 203/220 nt Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD5-18*01 F 3 8 8 15 8.8.15 [2.17.38.11] CARVGYGYRGIYFFDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 42 of V-REGION: 'A' (1 nucleotides at position 43 to 43 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 50 of V-REGION: 'CT' (2 nucleotides at position 67 to 68 in the user submitted sequence), (cause frameshift); agcctctacattcacgtttagtcggtattggatgagctgggtAccgccaggctccaggggaagggcCTgggagtgggtggccaacataaaggaagacggaagtgagagatattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgtgcgagagtgggctatggttaccgaggaatatacttctttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [301, 300] g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|c87>g|a106>c,S36>R(- - -)|c108>g,S36>R(- - -)|a142>g,K48>E(+ - -)|t149>g,L50>R(- + -)|c172>g,Q58>E(+ + -)|t177>c|a194>g,K65>R(+ + +)|c198>t|c246>t|a258>t|a271>t,M91>L(+ + -)|c280>t| g79>a,G27>T(+ - -)|g80>c,G27>T(+ - -)|c87>g|a106>c,S36>R(- - -)|c108>g,S36>R(- - -)| a142>g,K48>E(+ - -)|t149>g,L50>R(- + -)| c172>g,Q58>E(+ + -)|t177>c|a194>g,K65>R(+ + +)| c198>t|c246>t|a258>t|a271>t,M91>L(+ + -)|c280>t| 318 226 211 15 6 9 2 1 3 1 1 0 2 0 1 3 0 1 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 0 0 1 0 0 0 1 2 0 0 51 51 49 2 0 2 1 0 0 0 0 0 0 0 0 0 0 1 30 24 21 3 1 2 1 0 0 1 0 0 0 0 0 1 0 0 117 114 109 5 4 1 0 0 3 0 0 0 2 0 0 0 0 0 6 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct acattcacgtttagtcggtattgg atgagctgggtccgccaggctccaggggaagggcgggagtgggtggccaac ataaaggaagacggaagtgagaga tattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgt gcgagagtgggctatggttaccgaggaatatacttctttgactac ARVGYGYRGIYFFDY IGHV3-7 IGHD5-18 IGHJ4 acattcacgtttagtcggtattgg atgagctgggtccgccaggctccaggggaagggcgggagtgggtggccaac ataaaggaagacggaagtgagaga tattatgtggactctgtgaagggccgattcaccatctccagagacaatgccaagaactctctgtatctgcaattgaacagcttgagagccgaggacacggctgtgtattactgt gcgagagtgggctatggttaccgaggaatatacttctttgactac IGG2 15 213 7 8 4 8 3 7 4 2 3 6 JY8QFUQ01DECT5 11173 productive Homsap IGHV3-33*04 F (see comment) 693 80.18 174/217 nt NA Homsap IGHJ4*02 F 190 89.36 42/47 nt Homsap IGHD2-21*01 F 3 8 8 7 8.8.7 [1.17.38.11] CARDILDYW in-frame + Low V-REGION identity (80,18% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release atttggattccctttcagcgactatggcatgcactgggtccgccagactccagacaagggactagaaattgtggccattatctggcatgacggaagtcagcaattctatgcagactccgtgctgggtcgattcaccgtctccagagacaattccgacaacactctccagttgcagctgagcaggttgacagccgaagacacggctatttattattgtgcgagagatattttggactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtccgtggaactcag NA IGG2 100 94 [269, 270] g75>a|c77>t,S26>F(- - -)|a85>c,T29>P(+ + -)|c87>t,T29>P(+ + -)|t105>c|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g133>a,A45>T(- - -)|g140>a,G47>D(- - -)|g147>a|g153>a|t154>a,W52>I(+ - -)|g155>t,W52>I(+ - -)|g156>t,W52>I(+ - -)|a162>c|g163>a,V55>I(+ - +)|a168>c|t172>c,Y58>H(+ - -)|a190>c,N64>Q(+ - +)|t192>g,N64>Q(+ - +)|a193>c,K65>Q(+ - -)|a197>t,Y66>F(- + -)|a214>c,K72>L(- + -)|a215>t,K72>L(- + -)|c222>t|a232>g,I78>V(+ - +)|a250>g,K84>D(+ - -)|g252>c,K84>D(+ - -)|g258>t|g261>c|t262>c,Y88>Q(- - -)|t264>g,Y88>Q(- - -)|c265>t|a270>g|a271>c,M91>L(+ + -)|a275>g,N92>S(- - -)|c279>g,S93>R(- - -)|c280>t|g284>c,R95>T(- - -)|g291>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|c309>t| g75>a|c77>t,S26>F(- - -)| a85>c,T29>P(+ + -)|c87>t,T29>P(+ + -)|t105>c|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g133>a,A45>T(- - -)|g140>a,G47>D(- - -)|g147>a|g153>a|t154>a,W52>I(+ - -)|g155>t,W52>I(+ - -)|g156>t,W52>I(+ - -)|a162>c|g163>a,V55>I(+ - +)| a168>c|t172>c,Y58>H(+ - -)|a190>c,N64>Q(+ - +)|t192>g,N64>Q(+ - +)|a193>c,K65>Q(+ - -)| a197>t,Y66>F(- + -)|a214>c,K72>L(- + -)|a215>t,K72>L(- + -)|c222>t|a232>g,I78>V(+ - +)|a250>g,K84>D(+ - -)|g252>c,K84>D(+ - -)|g258>t|g261>c|t262>c,Y88>Q(- - -)|t264>g,Y88>Q(- - -)|c265>t|a270>g|a271>c,M91>L(+ + -)|a275>g,N92>S(- - -)|c279>g,S93>R(- - -)|c280>t|g284>c,R95>T(- - -)|g291>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|c309>t| 320 225 182 43 14 29 5 9 6 3 7 0 2 1 3 1 4 2 78 4 2 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 1 1 1 1 1 0 0 0 0 0 0 0 51 51 42 9 3 6 0 5 0 0 1 0 0 1 0 0 2 0 30 24 19 5 1 4 0 0 0 1 3 0 0 0 0 0 0 1 117 114 92 22 8 14 4 2 4 1 2 0 2 0 3 1 2 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| attt ggattccctttcagcgactatggc atgcactgggtccgccagactccagacaagggactagaaattgtggccatt atctggcatgacggaagtcagcaa ttctatgcagactccgtgctgggtcgattcaccgtctccagagacaattccgacaacactctccagttgcagctgagcaggttgacagccgaagacacggctatttattattgt gcgagagatattttggactac ARDILDY IGHV3-33 IGHD2-21 IGHJ4 ggattccctttcagcgactatggc atgcactgggtccgccagactccagacaagggactagaaattgtggccatt atctggcatgacggaagtcagcaa ttctatgcagactccgtgctgggtcgattcaccgtctccagagacaattccgacaacactctccagttgcagctgagcaggttgacagccgaagacacggctatttattattgt gcgagagatattttggactac IGG2 41 213 21 20 13 21 8 20 11 2 20 8 JY8QFUQ01DEK3I 18542 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 977 94.5 206/218 nt 94.04 205/218 nt Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD6-25*01 F 2 8 8 15 8.8.15 [1.17.38.11] CVRRGMVAGRYDAFDIW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 38 of V-REGION: 'G' (1 nucleotides at position 28 to 28 in the user submitted sequence), (cause frameshift); cctctggattcaccttcagtagttataGgcatgaactgggtccgcctggctccagggaaggggctggagtgggtctcggccattagtattactagtagttccacatattacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagacctcactgtttctgcaaatgaacagcctgagagccgaggacacggctctgtattactgtgtgagaagggggatggtagcgggcagatatgatgcttttgatatctggggccgagggactatggtcaccgtctctccagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctggggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG3 93 100 [296] c108>t|a131>t,Q44>L(- - -)|a162>g|t163>g,S55>A(- + -)|g173>t,S58>I(- - -)|g176>c,S59>T(+ - +)|a191>c,Y64>S(+ - -)|t194>c,I65>T(- - -)|c198>t|a254>c,N85>T(- + -)|a263>t,Y88>F(- + -)|g301>c,V101>L(+ - +)|c314>t,A105>V(+ - +)| c108>t| a131>t,Q44>L(- - -)|a162>g|t163>g,S55>A(- + -)| g173>t,S58>I(- - -)|g176>c,S59>T(+ - +)|a191>c,Y64>S(+ - -)|t194>c,I65>T(- - -)| c198>t|a254>c,N85>T(- + -)|a263>t,Y88>F(- + -)|g301>c,V101>L(+ - +)| c314>t,A105>V(+ - +)|g319>a|a320>g| 318 (320) 224 (226) 211 13 (15) 3 (5) 10 1 (2) 0 (1) 3 1 2 0 2 0 2 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 48 3 1 2 1 0 0 0 0 0 1 0 0 0 0 1 30 24 20 4 0 4 0 0 0 1 1 0 0 0 1 0 1 0 117 114 110 4 1 3 0 0 1 0 1 0 1 0 1 0 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 (1) 0 (1) 1 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtagttatagc atgaactgggtccgcctggctccagggaaggggctggagtgggtctcggcc attagtattactagtagttccaca tattacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagacctcactgtttctgcaaatgaacagcctgagagccgaggacacggctctgtattactgt gtgagaagggggatggtagcgggcagatatgatgcttttgatatc VRRGMVAGRYDAFDI IGHV3-21 IGHD6-25 IGHJ3 ggattcaccttcagtagttatagc atgaactgggtccgcctggctccagggaaggggctggagtgggtctcggcc attagtattactagtagttccaca tattacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagacctcactgtttctgcaaatgaacagcctgagagccgaggacacggctctgtattactgt gtgagaagggggatggtagcgggcagatatgatgcttttgatatc IGG3 12 213 4 8 2 5 2 7 2 1 5 4 JY8QFUQ01DEX6Z 13880 productive (see comment) Homsap IGHV4-61*02 F 873 89.4 194/217 nt 88.48 192/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD6-19*01 F 1 10 7 13 10.7.13 [0.16.38.11] CARQGDSSGPWNDYW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 102 of V-REGION: 'C' (1 nucleotides at position 211 to 211 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 50 of V-REGION: 1 nucleotides (from position 68 in the user submitted sequence), (cause frameshift) ccggtggctccatcagtactggttattactactggagctggatccggcagtccgccgggaagggactgaatggattgggcgcatgtctgccagaggggacagcaactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtaCtttttgtgcgagacaaggggatagtagtggaccctggaatgactactggggccagggaatcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [289] t78>c|c93>t|g101>c,S34>T(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c133>t,P45>S(+ - -)|g153>a|t165>c|c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>g,T65>S(+ - +)|a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| t78>c| c93>t|g101>c,S34>T(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)| c133>t,P45>S(+ - -)|g153>a|t165>c| c168>g,I56>M(+ + -)|a170>c,Y57>S(+ - -)|a172>g,T58>A(- - -)|t177>a,S59>R(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>g,T65>S(+ - +)| a214>c,K72>Q(+ - -)|a237>c|g238>a,V80>I(+ - +)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|c284>g,T95>S(+ - +)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g319>c| 318 (320) 223 (225) 200 (201) 23 (24) 5 (6) 18 3 4 3 2 3 0 2 1 2 (3) 3 0 0 78 2 1 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 30 26 4 1 3 0 1 1 0 0 0 1 0 1 0 0 0 51 50 47 3 2 1 0 1 1 1 0 0 0 0 0 0 0 0 30 21 14 7 0 7 2 1 0 0 1 0 0 1 0 2 0 0 117 114 106 8 1 7 1 1 1 0 2 0 1 0 1 1 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| cc ggtggctccatcagtactggttattactac tggagctggatccggcagtccgccgggaagggactgaatggattgggcgc atgtctgccagaggggacagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac ARQGDSSGPWNDY IGHV4-61 IGHD6-19 IGHJ4 ggtggctccatcagtactggttattactac tggagctggatccggcagtccgccgggaagggactgaatggattgggcgc atgtctgccagaggggacagc aactacaacccctccctccagagtcgagtcaccatatccatagacacgtccaagaaccagttctccctgaggctgacctctgtgagcgccgcagacacggccgtgtatttttgt gcgagacaaggggatagtagtggaccctggaatgactac IGG1 22 215 11 11 7 12 4 10 3 1 8 10 JY8QFUQ01DFG5Q 14218 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 836 87.91 189/215 nt 86.98 187/215 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-21*02 F 3 8 7 15 8.7.15 [1.17.38.11] CARGRTVVTVIDYIDYW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'A' (1 nucleotides at position 11 to 11 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 45 of V-REGION: 'A' (1 nucleotides at position 49 to 49 in the user submitted sequence), (cause frameshift); tctctgggggActccattagtggttactattggacgtggatccggcagAcccccagggaagggactggagtggattggaaatgtccattacactgggagtaccaagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgtgcgagaggacgaacggtggtgactgttattgactacattgactattggggtcagggagccctggtcagcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [293] t81>g|c90>t|a106>g,S36>G(+ + -)|c114>t|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|g162>a|t163>a,Y55>N(- - -)|a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|c192>t|c198>g,N66>K(+ - -)|a203>g,N68>S(- - -)|a234>g,I78>M(+ + -)|a240>t|c245>t,T82>M(- - -)|a251>g,K84>R(+ + +)|t262>a,S88>T(+ - +)|g267>c|g270>a|c271>t|g275>c,S92>T(+ - +)|g282>a|a283>g,T95>A(- - -)|g303>c| t81>g|c90>t|a106>g,S36>G(+ + -)|c114>t| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|g162>a|t163>a,Y55>N(- - -)| a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|c192>t| c198>g,N66>K(+ - -)|a203>g,N68>S(- - -)|a234>g,I78>M(+ + -)|a240>t|c245>t,T82>M(- - -)|a251>g,K84>R(+ + +)|t262>a,S88>T(+ - +)|g267>c|g270>a|c271>t|g275>c,S92>T(+ - +)|g282>a|a283>g,T95>A(- - -)|g303>c| a320>g| 319 (320) 222 (223) 196 26 (27) 11 (12) 15 6 (7) 3 5 1 0 0 1 2 5 2 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 3 1 1 0 2 0 0 0 0 0 0 0 0 1 51 51 47 4 1 3 0 1 0 0 0 0 0 1 1 1 0 0 30 21 17 4 1 3 1 0 1 1 0 0 0 0 1 0 0 0 117 114 100 14 6 8 4 2 2 0 0 0 1 1 3 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct gggggctccattagtggttactat tggacgtggatccggcagcccccagggaagggactggagtggattggaaat gtccattacactgggagtacc aagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttattgactacattgactat ARGRTVVTVIDYIDY IGHV4-59 IGHD2-21 IGHJ4 gggggctccattagtggttactat tggacgtggatccggcagcccccagggaagggactggagtggattggaaat gtccattacactgggagtacc aagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttattgactacattgactat IGA1 26 210 15 11 8 15 7 11 7 4 11 4 JY8QFUQ01DFJK6 15984 productive Homsap IGHV3-48*02 F, or Homsap IGHV3-48*04 F 944 93.49 201/215 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-18*01 F 3 8 8 13 8.8.13 [0.17.38.11] CARDRRGYSYEMDYW in-frame - ctggattcaccttcagtgtccatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcataccttagtagtggtagtgataccatatactacgcagactctgtgaggggccggttcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagtggcctgagagacgaggacacggctgtttattactgtgcgagagatcggcgtgggtatagttatgaaatggactactggggacagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [286] a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|t109>c,Y37>H(+ - -)|a166>c,I56>L(+ + +)|a175>g,S59>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a215>g,K72>R(+ + +)|a225>g|t246>c|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|a277>g,S93>G(+ + -)|g303>t| a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|t109>c,Y37>H(+ - -)| a166>c,I56>L(+ + +)|a175>g,S59>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)| a215>g,K72>R(+ + +)|a225>g|t246>c|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|a277>g,S93>G(+ + -)|g303>t| 320 223 209 14 3 11 7 1 1 2 1 0 0 0 0 0 2 0 78 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 0 0 1 0 0 0 0 0 0 1 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 2 1 0 0 1 0 0 0 0 0 0 0 117 114 107 7 3 4 4 0 1 1 0 0 0 0 0 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ct ggattcaccttcagtgtccatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac cttagtagtggtagtgataccata tactacgcagactctgtgaggggccggttcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagtggcctgagagacgaggacacggctgtttattactgt gcgagagatcggcgtgggtatagttatgaaatggactac ARDRRGYSYEMDY IGHV3-48 IGHD5-18 IGHJ4 ggattcaccttcagtgtccatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac cttagtagtggtagtgataccata tactacgcagactctgtgaggggccggttcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagtggcctgagagacgaggacacggctgtttattactgt gcgagagatcggcgtgggtatagttatgaaatggactac IGG2 14 213 11 3 2 4 9 10 3 0 4 7 JY8QFUQ01DFMQ1 13317 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 984 95.79 205/214 nt 95.33 204/214 nt Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD1-26*01 F 3 8 7 12 8.7.12 [1.17.38.11] CARWRSRSYDFDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 57 of V-REGION: 3 nucleotides (from position 83 in the user submitted sequence), (do not cause frameshift) ctctggattcacctttagcaactatgccatgacctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattacaagtggtggtagcacatactacgcaaactccgtgaagggccggttcaccgtctccagagacagttccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgagatggcgtagtcggagctacgactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [281] g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|c164>g,A55>G(- + -)|g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|t174>a,G58>T(+ - -)|g205>a,D69>N(+ + -)|a232>g,I78>V(+ - +)|a245>g,N82>S(- - -)| g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|c164>g,A55>G(- + -)| g172>a,G58>T(+ - -)|g173>c,G58>T(+ - -)|t174>a,G58>T(+ - -)| g205>a,D69>N(+ + -)|a232>g,I78>V(+ - +)|a245>g,N82>S(- - -)| a317>g,K106>R(+ + +)|g319>t|a320>g| 316 (320) 218 (222) 209 (210) 9 (12) 0 (2) 9 (10) 2 (4) 3 0 0 0 0 0 1 2 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 0 0 0 0 0 0 1 1 0 0 30 21 18 3 0 3 0 1 0 0 0 0 0 1 1 0 0 0 117 114 111 3 0 3 2 1 0 0 0 0 0 0 0 0 0 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (2) 0 0 0 0 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcaactatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attacaagtggtggtagcaca tactacgcaaactccgtgaagggccggttcaccgtctccagagacagttccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagatggcgtagtcggagctacgactttgactac ARWRSRSYDFDY IGHV3-23 IGHD1-26 IGHJ4 ggattcacctttagcaactatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attacaagtggtggtagcaca tactacgcaaactccgtgaagggccggttcaccgtctccagagacagttccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagatggcgtagtcggagctacgactttgactac IGA1 9 210 5 4 3 6 2 3 0 0 5 4 JY8QFUQ01DFNKY 9320 productive Homsap IGHV3-72*01 F 1088 98.66 221/224 nt NA Homsap IGHJ5*02 F (see comment) 183 84.31 43/51 nt Homsap IGHD2-15*01 F 1 8 10 8 8.10.8 [1.17.38.11] CLRPPRYDPW in-frame - Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) cctctggattaaccctcagtgaccactacatggactgggtccgccaggctccagggaaggggctggagtgggttggccgtactagaaacaaagctaacagttacaccacagaatacgccgcgtctgtgaagggcagattcaccatctcaagagatgattcaaagaactcactgtatctgcaaatgaacagcctgaaaaccgaggacacggccgtgtattactgtcttaggccgccgaggtacgacccctggggccaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [279] c84>a,F28>L(+ - -)|t88>c,F30>L(+ - -)|a216>g|g313>c,A105>L(+ - +)|c314>t,A105>L(+ - +)| c84>a,F28>L(+ - -)|t88>c,F30>L(+ - -)| a216>g| g313>c,A105>L(+ - +)|c314>t,A105>L(+ - +)|a318>g|g319>c|a320>c| 317 (320) 229 (232) 224 5 (8) 1 (4) 4 1 (2) 0 1 1 0 (1) 1 0 0 1 (2) 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 1 0 1 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 113 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 5 (8) 5 3 2 (5) 0 (3) 2 0 (1) 0 1 0 0 (1) 0 0 0 1 (2) 0 0 0 ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,165-168(FR2-CDR2)|agct,177-180(CDR2)|aact,253-256(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tgct,312-315(FR3)| cctct ggattaaccctcagtgaccactac atggactgggtccgccaggctccagggaaggggctggagtgggttggccgt actagaaacaaagctaacagttacaccaca gaatacgccgcgtctgtgaagggcagattcaccatctcaagagatgattcaaagaactcactgtatctgcaaatgaacagcctgaaaaccgaggacacggccgtgtattactgt cttaggccgccgaggtacgacccc LRPPRYDP IGHV3-72 IGHD2-15 IGHJ5 ggattaaccctcagtgaccactac atggactgggtccgccaggctccagggaaggggctggagtgggttggccgt actagaaacaaagctaacagttacaccaca gaatacgccgcgtctgtgaagggcagattcaccatctcaagagatgattcaaagaactcactgtatctgcaaatgaacagcctgaaaaccgaggacacggccgtgtattactgt cttaggccgccgaggtacgacccc IGA1 3 219 2 1 0 1 2 2 1 0 0 2 JY8QFUQ01DFY56 18673 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 860 88.53 193/218 nt NA Homsap IGHJ3*01 F (see comment) 169 82 41/50 nt Homsap IGHD2-15*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARGAGAEGKGGMDVW in-frame - Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) cctctggattcaccttcaaaaagtatggcatgaactggctccgccaggctccagggaaggggctggagtgggtcgcaaccattcgcagtagtggtacttccatacactatgccgactccgtgaagggccgattcactatcaccagagacaacgccaacaactcactgtatctgcaattgaacagcctgggagtcgaggactcggctgtgtatttctgtgcgagaggggccggcgcagaaggtaaaggtggtatggacgtctggggccaagggactacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA1 71 100 [291] g104>a,S35>K(- - -)|t105>a,S35>K(- - -)|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a112>g,S38>G(+ + -)|g124>c,V42>L(+ - +)|t160>g,S54>A(- + -)|t163>a,S55>T(+ - +)|a169>c,S57>R(- - -)|t171>c,S57>R(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|a191>c,Y64>S(+ - -)|t196>c,Y66>H(+ - -)|c201>t|a204>c|a210>c|c231>t|t235>a,S79>T(+ - +)|g252>c,K84>N(+ - -)|a271>t,M91>L(+ + -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|a295>t,T99>S(+ - +)|a308>t,Y103>F(- + -)| g104>a,S35>K(- - -)|t105>a,S35>K(- - -)|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a112>g,S38>G(+ + -)| g124>c,V42>L(+ - +)|t160>g,S54>A(- + -)|t163>a,S55>T(+ - +)| a169>c,S57>R(- - -)|t171>c,S57>R(- - -)|a184>g,S62>G(+ + -)|g188>c,S63>T(+ - +)|a191>c,Y64>S(+ - -)| t196>c,Y66>H(+ - -)|c201>t|a204>c|a210>c|c231>t|t235>a,S79>T(+ - +)|g252>c,K84>N(+ - -)|a271>t,M91>L(+ + -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|a295>t,T99>S(+ - +)|a308>t,Y103>F(- + -)| a320>g| 319 (320) 225 (226) 200 25 (26) 4 (5) 21 3 (4) 2 3 2 4 0 3 3 3 1 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 2 0 0 0 0 0 1 0 1 0 0 51 51 48 3 0 3 0 0 0 0 0 0 0 1 1 0 0 1 30 24 19 5 0 5 1 0 0 1 2 0 0 0 1 0 0 0 117 114 102 12 4 8 1 0 3 1 2 0 3 1 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcaaaaagtatggc atgaactggctccgccaggctccagggaaggggctggagtgggtcgcaacc attcgcagtagtggtacttccata cactatgccgactccgtgaagggccgattcactatcaccagagacaacgccaacaactcactgtatctgcaattgaacagcctgggagtcgaggactcggctgtgtatttctgt gcgagaggggccggcgcagaaggtaaaggtggtatggacgtc ARGAGAEGKGGMDV IGHV3-21 IGHD2-15 IGHJ3 ggattcaccttcaaaaagtatggc atgaactggctccgccaggctccagggaaggggctggagtgggtcgcaacc attcgcagtagtggtacttccata cactatgccgactccgtgaagggccgattcactatcaccagagacaacgccaacaactcactgtatctgcaattgaacagcctgggagtcgaggactcggctgtgtatttctgt gcgagaggggccggcgcagaaggtaaaggtggtatggacgtc IGA1 25 213 10 15 5 9 5 16 4 0 11 10 JY8QFUQ01DG3GX 3558 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 982 94.52 207/219 nt 94.06 206/219 nt Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD6-13*01 F 2 8 8 16 8.8.16 [2.17.38.11] CARGRIVSVGMSGWFDPW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'AG' (2 nucleotides at position 60 to 61 in the user submitted sequence), (cause frameshift); gcctctggattcaccttcagtcagtactggatgtactgggtccgccaagctccagggaaAGggggctggtgtgggtctcacgtattaatggtgatggaagtagcacaagctatgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtatattattgtgcaagaggccgtatagtatcagttgggatgtcaggctggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [300, 299] a106>c,S36>Q(- - -)|g107>a,S36>Q(- - -)|c108>g,S36>Q(- - -)|c118>t,H40>Y(+ - -)|a172>g,S58>G(+ + -)|g186>a|c201>t|g258>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>a|c309>t| a106>c,S36>Q(- - -)|g107>a,S36>Q(- - -)|c108>g,S36>Q(- - -)| c118>t,H40>Y(+ - -)| a172>g,S58>G(+ + -)|g186>a| c201>t|g258>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>a|c309>t| a320>g| 319 (320) 226 (227) 214 12 (13) 5 (6) 7 1 (2) 3 4 0 1 0 0 0 2 1 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 1 0 0 0 0 1 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 22 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 117 114 108 6 4 2 0 1 3 0 0 0 0 0 2 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcaccttcagtcagtactgg atgtactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attaatggtgatggaagtagcaca agctatgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtatattattgt gcaagaggccgtatagtatcagttgggatgtcaggctggttcgacccc ARGRIVSVGMSGWFDP IGHV3-74 IGHD6-13 IGHJ5 ggattcaccttcagtcagtactgg atgtactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attaatggtgatggaagtagcaca agctatgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtatattattgt gcaagaggccgtatagtatcagttgggatgtcaggctggttcgacccc IGA2 12 213 8 4 7 10 1 2 4 1 3 4 JY8QFUQ01DG853 13675 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 927 92.17 200/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-16*02 F 1 8 8 14 8.8.14 [1.17.38.11] CAKVGLMSARDRIDYW in-frame + ctctggattcacctttagcaactatgccatgagttgggtccgccaggctcaagggaaggggctggactgggtctcagatattagtaatagtggtggtgacacattctacgcaggctccgtgaagggccgcttcaccatctccagagacaattccaggaacactctatatctgcaaatggacagcctgagagccgaggacacggccgtgtattactgtgcgaaagtggggctgatgtccgctcgagaccgcattgactactggggccggggaaccctggtctccgtctcctcaccatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA IGA1 100 100 [290] g107>a,S36>N(- - -)|c120>t|c137>a,P46>Q(- - -)|g153>c,E51>D(+ - +)|c164>a,A55>D(- - -)|g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a197>t,Y66>F(- + -)|a206>g,D69>G(- - -)|g225>c|a251>g,K84>R(+ + +)|g258>t|g261>a|a274>g,N92>D(+ + -)|a303>g| g107>a,S36>N(- - -)| c120>t|c137>a,P46>Q(- - -)|g153>c,E51>D(+ - +)|c164>a,A55>D(- - -)| g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a197>t,Y66>F(- + -)|a206>g,D69>G(- - -)|g225>c|a251>g,K84>R(+ + +)|g258>t|g261>a|a274>g,N92>D(+ + -)|a303>g| a320>t| 319 (320) 224 (225) 207 17 (18) 5 (6) 12 5 5 1 0 0 2 1 (2) 0 2 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 47 4 1 3 0 0 1 0 0 2 0 0 1 0 0 0 30 24 20 4 0 4 1 3 0 0 0 0 0 0 0 0 0 0 117 114 106 8 4 4 4 1 0 0 0 0 1 0 1 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcaactatgcc atgagttgggtccgccaggctcaagggaaggggctggactgggtctcagat attagtaatagtggtggtgacaca ttctacgcaggctccgtgaagggccgcttcaccatctccagagacaattccaggaacactctatatctgcaaatggacagcctgagagccgaggacacggccgtgtattactgt gcgaaagtggggctgatgtccgctcgagaccgcattgactac AKVGLMSARDRIDY IGHV3-23 IGHD3-16 IGHJ4 ggattcacctttagcaactatgcc atgagttgggtccgccaggctcaagggaaggggctggactgggtctcagat attagtaatagtggtggtgacaca ttctacgcaggctccgtgaagggccgcttcaccatctccagagacaattccaggaacactctatatctgcaaatggacagcctgagagccgaggacacggccgtgtattactgt gcgaaagtggggctgatgtccgctcgagaccgcattgactac IGA1 17 213 11 6 6 11 5 6 5 0 7 5 JY8QFUQ01DGFLY 1370 productive Homsap IGHV1-69*08 F 930 92.99 199/214 nt NA Homsap IGHJ4*03 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 3 8 8 14 8.8.14 [0.17.38.11] CARDSNGYSSGYIDCW in-frame + tggaggctccttcggcagctacactatcacctgggtgcgacaggcccctggacaagggcttgagtggatgggaaggatcacccctatccttggttcaacaagctactcacagaagttccagggcagagtcacgattaccgcggacacattcacgggcacagcctacatggagctgagcagcctgacatctgaagacacggccgtatattactgtgcgcgagactcgaatggatacagttctggttatattgactgctggggccaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 79 95 [287, 286] a85>t,T29>S(+ - +)|a103>g,S35>G(+ + -)|t111>c|g119>c,S40>T(+ - +)|t170>c,I57>T(- - -)|a190>t,T64>S(+ - +)|g193>a,A65>T(- - -)|a197>g,N66>S(- - -)|g202>t,A68>S(- + -)|a245>c,K82>T(- - -)|c248>t,S83>F(- - -)|a253>g,S85>G(+ + -)|g284>c,R95>T(- - -)|g291>a|g303>a|a316>c| a85>t,T29>S(+ - +)|a103>g,S35>G(+ + -)|t111>c| g119>c,S40>T(+ - +)| t170>c,I57>T(- - -)|a190>t,T64>S(+ - +)|g193>a,A65>T(- - -)| a197>g,N66>S(- - -)|g202>t,A68>S(- + -)|a245>c,K82>T(- - -)|c248>t,S83>F(- - -)|a253>g,S85>G(+ + -)|g284>c,R95>T(- - -)|g291>a|g303>a| a316>c| 320 222 206 16 4 12 3 3 1 2 2 0 2 0 2 0 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 1 0 0 1 0 0 1 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 21 3 0 3 0 1 0 1 0 0 1 0 0 0 0 0 117 114 106 8 2 6 2 2 1 0 1 0 0 0 1 0 1 0 8 8 7 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 aa,17-18(FR1)|aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,162-163(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|tt,185-186(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggca,82-85(CDR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agca,192-195(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,111-114(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggaggctccttcggcagctacact atcacctgggtgcgacaggcccctggacaagggcttgagtggatgggaagg atcacccctatccttggttcaaca agctactcacagaagttccagggcagagtcacgattaccgcggacacattcacgggcacagcctacatggagctgagcagcctgacatctgaagacacggccgtatattactgt gcgcgagactcgaatggatacagttctggttatattgactgc ARDSNGYSSGYIDC IGHV1-69 IGHD5-18 IGHJ4 ggaggctccttcggcagctacact atcacctgggtgcgacaggcccctggacaagggcttgagtggatgggaagg atcacccctatccttggttcaaca agctactcacagaagttccagggcagagtcacgattaccgcggacacattcacgggcacagcctacatggagctgagcagcctgacatctgaagacacggccgtatattactgt gcgcgagactcgaatggatacagttctggttatattgactgc IGA2 15 213 9 6 4 7 5 8 2 1 7 5 JY8QFUQ01DGFY7 4099 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 990 95.39 207/217 nt NA Homsap IGHJ3*02 F 232 96 48/50 nt Homsap IGHD2-21*02 F 2 8 8 15 8.8.15 [1.17.38.11] CARLCGNDCRNDVFDIW in-frame - ctctggattcaccttcagtacctactggatgcactgggtccgccaagttccagggaaggggctggtgtgggtctcacgtgttgatagtgatgggactagcacagtctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctatatctgcaaatgaacagtctgaaagccgaggacacggctgtatattactgtgcaagattatgtggtaatgactgccgcaatgatgtttttgatatctggggccaagggacattggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [293] g107>c,S36>T(+ - +)|c134>t,A45>V(+ - +)|a166>g,I56>V(+ - +)|a169>g,N57>D(+ + -)|g188>c,S63>T(+ - +)|a196>g,S66>V(- - -)|g197>t,S66>V(- - -)|g261>a|g284>a,R95>K(+ + +)|g303>a| g107>c,S36>T(+ - +)| c134>t,A45>V(+ - +)| a166>g,I56>V(+ - +)|a169>g,N57>D(+ + -)|g188>c,S63>T(+ - +)| a196>g,S66>V(- - -)|g197>t,S66>V(- - -)|g261>a|g284>a,R95>K(+ + +)|g303>a| g319>t|a320>t| 318 (320) 223 (225) 213 10 (12) 2 (4) 8 3 3 1 0 0 0 0 (1) 0 2 0 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 2 0 0 0 0 0 0 0 1 0 0 0 117 114 109 5 2 3 1 3 0 0 0 0 0 0 0 0 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtacctactgg atgcactgggtccgccaagttccagggaaggggctggtgtgggtctcacgt gttgatagtgatgggactagcaca gtctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctatatctgcaaatgaacagtctgaaagccgaggacacggctgtatattactgt gcaagattatgtggtaatgactgccgcaatgatgtttttgatatc ARLCGNDCRNDVFDI IGHV3-74 IGHD2-21 IGHJ3 ggattcaccttcagtacctactgg atgcactgggtccgccaagttccagggaaggggctggtgtgggtctcacgt gttgatagtgatgggactagcaca gtctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctatatctgcaaatgaacagtctgaaagccgaggacacggctgtatattactgt gcaagattatgtggtaatgactgccgcaatgatgtttttgatatc IGA1 10 213 7 3 4 7 3 3 2 0 4 4 JY8QFUQ01DH08T 11721 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 908 91.63 197/215 nt NA Homsap IGHJ3*02 F 214 92 46/50 nt Homsap IGHD2-21*01 F 2 8 7 15 8.7.15 [1.17.38.11] CTRELRLAAARDGVDIW in-frame - cctctgggttcaccgtcagtagcaagttcatgagctgggtccgccagggtccagggaaggggctggagtgggtctcagttacttatcctgatggtactacacattatagagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgtacgagagaacttaggctggctgctgcgcgtgatggtgttgacatttggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [291] c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)|c134>g,A45>G(- + -)|t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|t262>g,Y88>D(- - -)|g303>t|c309>t|g313>a,A105>T(- - -)| c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)| c134>g,A45>G(- + -)| t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)| t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|t262>g,Y88>D(- - -)|g303>t|c309>t| g313>a,A105>T(- - -)| 320 223 204 19 3 16 0 3 5 2 1 0 1 0 2 3 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 21 14 7 0 7 0 1 2 1 1 0 0 0 2 0 0 0 117 114 106 8 3 5 0 1 3 1 0 0 0 0 0 1 1 1 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct gggttcaccgtcagtagcaagttc atgagctgggtccgccagggtccagggaaggggctggagtgggtctcagtt acttatcctgatggtactaca cattatagagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttaggctggctgctgcgcgtgatggtgttgacatt TRELRLAAARDGVDI IGHV3-53 IGHD2-21 IGHJ3 gggttcaccgtcagtagcaagttc atgagctgggtccgccagggtccagggaaggggctggagtgggtctcagtt acttatcctgatggtactaca cattatagagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttaggctggctgctgcgcgtgatggtgttgacatt IGA1 18 210 9 9 7 13 2 5 3 0 6 9 JY8QFUQ01DH0MW 5431 productive Homsap IGHV3-74*03 F 1031 97.25 212/218 nt NA Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD3-16*01 F 2 8 8 10 8.8.10 [1.17.38.11] CATGPDYAYAMW in-frame - cctctggattcaccttcagtcgctactggatgcactgggtccgccaagctccagggaagggcctggtgtgggtctcacgtattaaaagtgatgggattagcacaacgtacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacggtgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtactactgtgcaaccgggcccgattatgcgtatgctatgtggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 79 95 [279, 278] a106>c,S36>R(- - -)|g147>c|t171>a,N57>K(+ - -)|g188>t,S63>I(- - -)|c259>g,L87>V(+ - +)|t306>c| a106>c,S36>R(- - -)| g147>c| t171>a,N57>K(+ - -)|g188>t,S63>I(- - -)| c259>g,L87>V(+ - +)|t306>c| g317>c,R106>T(- - -)|a318>c,R106>T(- - -)|a320>g| 316 (320) 222 (226) 216 (217) 6 (9) 2 (3) 4 (6) 0 (1) 0 0 1 1 (2) 0 0 1 1 (2) 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 0 1 0 0 1 0 117 114 112 2 1 1 0 0 0 1 0 0 0 0 0 1 0 0 4 (8) 4 4 (5) 0 (3) 0 (1) 0 (2) 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtcgctactgg atgcactgggtccgccaagctccagggaagggcctggtgtgggtctcacgt attaaaagtgatgggattagcaca acgtacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacggtgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtactactgt gcaaccgggcccgattatgcgtatgctatg ATGPDYAYAM IGHV3-74 IGHD3-16 IGHJ3 ggattcaccttcagtcgctactgg atgcactgggtccgccaagctccagggaagggcctggtgtgggtctcacgt attaaaagtgatgggattagcaca acgtacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacggtgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtactactgt gcaaccgggcccgattatgcgtatgctatg IGA2 6 213 1 5 0 3 1 3 2 0 1 3 JY8QFUQ01DHC55 6219 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 855 88.48 192/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-15*01 F 3 8 8 14 8.8.14 [1.17.38.11] CAKEEGATGVYYVDHW in-frame + ctctggattcacctttaccacctccgccatggcctgggtccgccaggttccagggaaggggctggagtgggtctcaactattagacctagtggtgagagaacctactacgcagagtccgtgaggggccgcttcaccatctccagagacaattccgagaacacgttgtatctacaactgaacaacctgagagtcgaggacacggccatatattactgtgcgaaggaggaaggtgctactggagtctattatgttgaccactggggccagggaacccgggtcaccgtctcctcatcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 86 94 [291] g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|g163>a,A55>T(- - -)|t171>a,S57>R(- - -)|g172>c,G58>P(+ - -)|g173>c,G58>P(+ - -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)|a195>c|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|g225>c|a250>g,K84>E(+ - -)|c259>t|g267>a|a271>c,M91>L(+ + -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|a318>g| g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|g163>a,A55>T(- - -)| t171>a,S57>R(- - -)|g172>c,G58>P(+ - -)|g173>c,G58>P(+ - -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)|a195>c| c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|g225>c|a250>g,K84>E(+ - -)|c259>t|g267>a|a271>c,M91>L(+ + -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)| a318>g| 320 225 199 26 5 21 4 5 3 1 3 1 0 1 6 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 1 1 0 0 0 2 0 0 0 51 51 47 4 0 4 1 1 1 0 0 0 0 0 1 0 0 0 30 24 17 7 1 6 0 1 0 0 1 1 0 1 2 0 0 1 117 114 104 10 3 7 2 3 2 0 1 0 0 0 1 1 0 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaccacctccgcc atggcctgggtccgccaggttccagggaaggggctggagtgggtctcaact attagacctagtggtgagagaacc tactacgcagagtccgtgaggggccgcttcaccatctccagagacaattccgagaacacgttgtatctacaactgaacaacctgagagtcgaggacacggccatatattactgt gcgaaggaggaaggtgctactggagtctattatgttgaccac AKEEGATGVYYVDH IGHV3-23 IGHD2-15 IGHJ4 ggattcacctttaccacctccgcc atggcctgggtccgccaggttccagggaaggggctggagtgggtctcaact attagacctagtggtgagagaacc tactacgcagagtccgtgaggggccgcttcaccatctccagagacaattccgagaacacgttgtatctacaactgaacaacctgagagtcgaggacacggccatatattactgt gcgaaggaggaaggtgctactggagtctattatgttgaccac IGG1 25 213 12 13 8 16 4 9 3 1 11 10 JY8QFUQ01DHH0D 5192 productive Homsap IGHV3-72*01 F 966 92.83 207/223 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-3*01 F 3 8 10 11 8.10.11 [1.17.38.11] CITSFGGRSLAYW in-frame + ctctggattcaccttaagtgatcactacatggactgggtccgccaggctccagggaaggggctggagtgggttggccgtactaaaaacaaagctaacggttacactacacactacgccgcgtctgtgagaggcagattcattctttcaagagacgattcaaagaactcagtgtatctgcaaatgaacagcctgaaaatcgaggacacggccgtctattactgtattacttcttttggtggaagatccctggcgtactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG3 79 94 [288] c90>a,F30>L(+ - -)|c108>t|g170>a,R57>K(+ + +)|a184>g,S62>G(+ + -)|c192>t|g196>c,E66>H(- + -)|a198>c,E66>H(- + -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|c231>t,T77>I(- - -)|a232>c,I78>L(+ + +)|c234>t,I78>L(+ + +)|t243>c|c259>g,L87>V(+ - +)|c287>t,T96>I(- - -)|g303>c| c90>a,F30>L(+ - -)|c108>t| g170>a,R57>K(+ + +)|a184>g,S62>G(+ + -)|c192>t| g196>c,E66>H(- + -)|a198>c,E66>H(- + -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|c231>t,T77>I(- - -)|a232>c,I78>L(+ + +)|c234>t,I78>L(+ + +)|t243>c|c259>g,L87>V(+ - +)|c287>t,T96>I(- - -)|g303>c| g313>a,A105>I(+ - +)|c314>t,A105>I(+ - +)|g317>c,R106>T(- - -)|a318>t,R106>T(- - -)|g319>t|a320>c| 312 (320) 223 (231) 207 (209) 16 (22) 4 (6) 12 (16) 2 1 (2) 6 (7) 1 2 (3) 1 0 (1) 0 2 (3) 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 1 0 0 1 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 27 3 1 2 1 1 1 0 0 0 0 0 0 0 0 0 117 114 103 11 2 9 1 0 4 1 2 0 0 0 2 1 0 0 0 (8) 0 0 (2) 0 (6) 0 (2) 0 (4) 0 0 (1) 0 (1) 0 0 (1) 0 0 (1) 0 0 (1) 0 0 (1) 0 ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,180-181(CDR2)|ta,187-188(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,180-181(CDR2)|tt,186-187(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agct,177-180(CDR2)|agtt,184-187(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,165-168(FR2-CDR2)|agct,177-180(CDR2)|aact,253-256(FR3)|agcc,277-280(FR3)|aacc,285-288(FR3)|tact,307-310(FR3)|tgct,312-315(FR3)| ctct ggattcaccttaagtgatcactac atggactgggtccgccaggctccagggaaggggctggagtgggttggccgt actaaaaacaaagctaacggttacactaca cactacgccgcgtctgtgagaggcagattcattctttcaagagacgattcaaagaactcagtgtatctgcaaatgaacagcctgaaaatcgaggacacggccgtctattactgt attacttcttttggtggaagatccctggcgtac ITSFGGRSLAY IGHV3-72 IGHD3-3 IGHJ4 ggattcaccttaagtgatcactac atggactgggtccgccaggctccagggaaggggctggagtgggttggccgt actaaaaacaaagctaacggttacactaca cactacgccgcgtctgtgagaggcagattcattctttcaagagacgattcaaagaactcagtgtatctgcaaatgaacagcctgaaaatcgaggacacggccgtctattactgt attacttcttttggtggaagatccctggcgtac IGG3 16 219 10 6 7 11 3 5 2 2 9 3 JY8QFUQ01DHJ30 12471 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 1065 97.76 218/223 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD4-17*01 F 3 8 10 15 8.10.15 [1.17.38.11] CTTFGLTTVTKGYFDFW in-frame + ctctggattcactttcaataacgcctggatgagctgggtccgccaggctccagggaaggggctggagtgggttggccgtattaaaagcaaaactgatggtgggacaacagagtacgctgcacccgtgaaaggcagatttaccatctcaagagatgattcaaaaaacacgctttatctgcaaatgaacagcctgaaaatcgaggacacagccgtgtattactgtaccacattcggcctgactacggtgactaaaggctactttgacttctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA IGA2 100 100 [299] g104>a,S35>N(- - -)|c198>g,D66>E(+ - +)|c228>t|g261>t|c287>t,T96>I(- - -)| g104>a,S35>N(- - -)| c198>g,D66>E(+ - +)|c228>t|g261>t|c287>t,T96>I(- - -)| g319>t|a320>t| 318 (320) 229 (231) 224 5 (7) 2 (4) 3 0 1 2 0 0 0 0 (1) 0 0 1 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 110 4 2 2 0 0 2 0 0 0 0 0 0 1 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| ctct ggattcactttcaataacgcctgg atgagctgggtccgccaggctccagggaaggggctggagtgggttggccgt attaaaagcaaaactgatggtgggacaaca gagtacgctgcacccgtgaaaggcagatttaccatctcaagagatgattcaaaaaacacgctttatctgcaaatgaacagcctgaaaatcgaggacacagccgtgtattactgt accacattcggcctgactacggtgactaaaggctactttgacttc TTFGLTTVTKGYFDF IGHV3-15 IGHD4-17 IGHJ4 ggattcactttcaataacgcctgg atgagctgggtccgccaggctccagggaaggggctggagtgggttggccgt attaaaagcaaaactgatggtgggacaaca gagtacgctgcacccgtgaaaggcagatttaccatctcaagagatgattcaaaaaacacgctttatctgcaaatgaacagcctgaaaatcgaggacacagccgtgtattactgt accacattcggcctgactacggtgactaaaggctactttgacttc IGA2 5 219 3 2 3 5 0 0 2 0 2 1 JY8QFUQ01DI98R 16666 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 870 88.64 195/220 nt 88.18 194/220 nt Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD1-26*01 F 1 8 8 16 8.8.16 [2.17.38.11] CAKGIVGASMQADWFHPW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 72 of V-REGION: 'AG' (2 nucleotides at position 127 to 128 in the user submitted sequence), (cause frameshift); agcctctggattcgtctttggcgactatgcgatgagctgggtccggcaggctccagggagggggctggagtgggtctcaagtattagtggtagtggtgtcagcacatactacgtgggctccgtgaaAGgggccgcttcaccatctccagagacaattccaagaatgtgttgtatctgcaaatgaacggcctgagagtcgaggacacggccacatatcactgtgcgaaaggtatagtgggagcttctatgcaagccgactggttccacccctggggccaggggaccctgctcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [301] a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|a103>g,S35>G(+ + -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c114>g|c129>g|a143>g,K48>R(+ + +)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|g188>t,G63>V(- - -)|t189>c,G63>V(- - -)|c203>t,A68>V(+ - +)|a204>g,A68>V(+ - +)|a206>g,D69>G(- - -)|g225>c|c255>t|a256>g,T86>V(- - -)|c257>t,T86>V(- - -)|c259>t|a277>g,S93>G(+ + -)|c287>t,A96>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|t307>c,Y103>H(+ - -)| a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|a103>g,S35>G(+ + -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c114>g| c129>g|a143>g,K48>R(+ + +)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| g188>t,G63>V(- - -)|t189>c,G63>V(- - -)| c203>t,A68>V(+ - +)|a204>g,A68>V(+ - +)|a206>g,D69>G(- - -)|g225>c|c255>t|a256>g,T86>V(- - -)|c257>t,T86>V(- - -)|c259>t|a277>g,S93>G(+ + -)|c287>t,A96>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|t307>c,Y103>H(+ - -)| a320>g| 319 (320) 227 (228) 202 25 (26) 5 (6) 20 8 (9) 3 6 3 0 0 0 0 1 3 1 0 78 7 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 1 5 3 1 1 0 0 0 0 0 0 1 0 0 51 51 47 4 1 3 1 1 0 0 0 0 0 0 0 2 0 0 30 24 22 2 0 2 0 0 0 1 0 0 0 0 0 0 1 0 117 114 101 13 3 10 4 1 5 2 0 0 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| agcctct ggattcgtctttggcgactatgcg atgagctgggtccggcaggctccagggagggggctggagtgggtctcaagt attagtggtagtggtgtcagcaca tactacgtgggctccgtgaagggccgcttcaccatctccagagacaattccaagaatgtgttgtatctgcaaatgaacggcctgagagtcgaggacacggccacatatcactgt gcgaaaggtatagtgggagcttctatgcaagccgactggttccacccc AKGIVGASMQADWFHP IGHV3-23 IGHD1-26 IGHJ5 ggattcgtctttggcgactatgcg atgagctgggtccggcaggctccagggagggggctggagtgggtctcaagt attagtggtagtggtgtcagcaca tactacgtgggctccgtgaagggccgcttcaccatctccagagacaattccaagaatgtgttgtatctgcaaatgaacggcctgagagtcgaggacacggccacatatcactgt gcgaaaggtatagtgggagcttctatgcaagccgactggttccacccc IGA2 25 213 20 5 9 14 11 11 4 1 13 7 JY8QFUQ01DIWKI 1135 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 1009 95.89 210/219 nt NA Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [2.17.38.11] CAKDGARGGGSCFNSW in-frame - Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) gcctctggattcacctttagaagctatgccatgagctgggtccgccaggttccagggaaggggctggagtgggtctcagctattagtggtaatggtgctaacacatactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgtgcgaaagatggcgctagaggtggcggcagttgctttaactcctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG3 93 100 [293] c105>a,S35>R(- - -)|c134>t,A45>V(+ - +)|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|g191>a,S64>N(- - -)|g225>a|c248>t,S83>F(- - -)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)| c105>a,S35>R(- - -)| c134>t,A45>V(+ - +)| g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|g191>a,S64>N(- - -)| g225>a|c248>t,S83>F(- - -)|a263>c,Y88>S(+ - -)|g301>c,V101>L(+ - +)| 320 227 218 9 1 8 0 3 2 0 1 1 0 0 2 0 0 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 2 0 0 0 0 0 0 1 0 0 0 117 114 110 4 1 3 0 1 1 0 1 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcacctttagaagctatgcc atgagctgggtccgccaggttccagggaaggggctggagtgggtctcagct attagtggtaatggtgctaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtggcggcagttgctttaactcc AKDGARGGGSCFNS IGHV3-23 IGHD5-24 IGHJ4 ggattcacctttagaagctatgcc atgagctgggtccgccaggttccagggaaggggctggagtgggtctcagct attagtggtaatggtgctaacaca tactacgcagactccgtgaagggccgattcaccatctccagagacaatttcaagaacacgctgtctctgcaaatgaacagcctgagagccgaggacacggccctatattactgt gcgaaagatggcgctagaggtggcggcagttgctttaactcc IGG3 9 213 5 4 5 8 0 1 1 0 4 4 JY8QFUQ01DJ168 2126 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 944 93.49 201/215 nt 93.02 200/215 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 3 8 8 13 8.8.13 [1.17.37.11] CARGLAVILGGFDYW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 84 of V-REGION: 1 nucleotides (from position 154 in the user submitted sequence), (cause frameshift) tctggattcaccttcagtagttatagcatgcactgggtccgccaggctccagggaagggactggagtgggtctcctccattagtagtaatagtgcctacacagaccacgcagactcagtgaagggccgattcaccatctccagagacaacgacagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagggctagcagtgattcttggaggatttgactactggggccagggaaacctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [286] c108>t|a118>c,N40>H(- - -)|g147>a|a162>c|g176>a,S59>N(- - -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>c,S63>A(- + -)|t194>c,I65>T(- - -)|t196>g,Y66>D(- - -)|t199>c,Y67>H(+ - -)|c248>a,A83>D(- - -)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)| c108>t| a118>c,N40>H(- - -)|g147>a|a162>c| g176>a,S59>N(- - -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>c,S63>A(- + -)|t194>c,I65>T(- - -)| t196>g,Y66>D(- - -)|t199>c,Y67>H(+ - -)|c248>a,A83>D(- - -)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)| a320>g| 319 (320) 222 (223) 208 14 (15) 3 (4) 11 2 (3) 2 1 3 2 1 0 0 1 1 0 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 0 0 2 0 0 0 0 0 0 0 30 24 19 5 0 5 1 1 0 2 0 0 0 0 1 0 0 0 117 113 108 5 0 5 1 0 0 1 0 1 0 0 0 1 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcagtagttatagc atgcactgggtccgccaggctccagggaagggactggagtgggtctcctcc attagtagtaatagtgcctacaca gaccacgcagactcagtgaagggccgattcaccatctccagagacaacgacagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggctagcagtgattcttggaggatttgactac ARGLAVILGGFDY IGHV3-21 IGHD3-22 IGHJ4 ggattcaccttcagtagttatagc atgcactgggtccgccaggctccagggaagggactggagtgggtctcctcc attagtagtaatagtgcctacaca gaccacgcagactcagtgaagggccgattcaccatctccagagacaacgacagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggctagcagtgattcttggaggatttgactac IGG1 14 212 8 6 3 6 5 8 2 1 6 5 JY8QFUQ01DJCKC 3929 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD1-26*01 F 1 8 8 14 8.8.14 [1.17.38.11] CVKDLGLVGATGLDYW in-frame + ctctggattcacctttcgcagatatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattaataatagtggtggtagcacatactacgcagactccgtgcagggccggttcaccatctccagagacaattccaagaacacggtgtatctgcaactgagcagcctgagagccgaggacacggccatgtatttctgtgtgaaagatctgggactagtgggcgctactgggctggactactggggccagggaacccgggtcaccgtctccgcagcatccccgaccagccccaaggtcttcccggctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 95 [291, 290] a103>c,S35>R(- - -)|c108>a,S36>R(- - -)|c164>g,A55>G(- + -)|g170>a,S57>N(- - -)|g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|a214>c,K72>Q(+ - -)|c259>g,L87>V(+ - +)|a271>c,M91>L(+ + -)|a275>g,N92>S(- - -)|g301>a,V101>M(+ - -)|a303>g,V101>M(+ - -)|a308>t,Y103>F(- + -)|c314>t,A105>V(+ - +)| a103>c,S35>R(- - -)|c108>a,S36>R(- - -)| c164>g,A55>G(- + -)| g170>a,S57>N(- - -)|g172>a,G58>N(- - -)|g173>a,G58>N(- - -)| a214>c,K72>Q(+ - -)|c259>g,L87>V(+ - +)|a271>c,M91>L(+ + -)|a275>g,N92>S(- - -)|g301>a,V101>M(+ - -)|a303>g,V101>M(+ - -)|a308>t,Y103>F(- + -)| c314>t,A105>V(+ - +)| 320 225 211 14 0 14 2 4 1 0 3 1 1 0 0 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 1 1 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 21 3 0 3 0 3 0 0 0 0 0 0 0 0 0 0 117 114 107 7 0 7 2 1 0 0 2 0 1 0 0 1 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttcgcagatatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attaataatagtggtggtagcaca tactacgcagactccgtgcagggccggttcaccatctccagagacaattccaagaacacggtgtatctgcaactgagcagcctgagagccgaggacacggccatgtatttctgt gtgaaagatctgggactagtgggcgctactgggctggactac VKDLGLVGATGLDY IGHV3-23 IGHD1-26 IGHJ4 ggattcacctttcgcagatatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attaataatagtggtggtagcaca tactacgcagactccgtgcagggccggttcaccatctccagagacaattccaagaacacggtgtatctgcaactgagcagcctgagagccgaggacacggccatgtatttctgt gtgaaagatctgggactagtgggcgctactgggctggactac IGA2 13 213 6 7 4 7 2 6 0 0 8 5 JY8QFUQ01DJQ7I 17917 productive Homsap IGHV3-7*01 F 968 94.04 205/218 nt NA Homsap IGHJ4*02 F 176 86.96 40/46 nt Homsap IGHD7-27*01 F 1 8 8 6 8.8.6 [1.17.38.11] CSRLLEEW in-frame + cctctggtttcacctttagtaacgattggatggactgggtccgccaggctccagggaaggggctggagtgggtggccaatataaagggagatggaagtgagaaaaactatgtagactctgcgaagggccgattcatcatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgttcgagactccttgaggaatggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtcccagggctnttcccc NA IGA1 100 100 [267] a81>t|g107>a,S36>N(- - -)|t109>g,Y37>D(- - -)|a118>g,S40>D(- - -)|g119>a,S40>D(- - -)|c165>t|c172>g,Q58>G(- - -)|a173>g,Q58>G(- - -)|t196>a,Y66>N(- - -)|g204>a|t212>c,V71>A(+ - +)|c230>t,T77>I(- - -)|c287>t,A96>V(+ - +)|g313>t,A105>S(- + -)| a81>t|g107>a,S36>N(- - -)|t109>g,Y37>D(- - -)| a118>g,S40>D(- - -)|g119>a,S40>D(- - -)|c165>t| c172>g,Q58>G(- - -)|a173>g,Q58>G(- - -)| t196>a,Y66>N(- - -)|g204>a|t212>c,V71>A(+ - +)|c230>t,T77>I(- - -)|c287>t,A96>V(+ - +)| g313>t,A105>S(- + -)|g319>c|a320>t| 318 (320) 224 (226) 210 14 (16) 3 (5) 11 2 3 3 1 0 0 1 (2) 1 0 (1) 1 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 0 0 0 0 1 0 0 0 0 1 51 51 48 3 1 2 1 1 1 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 1 0 0 117 114 109 5 1 4 0 1 2 1 0 0 0 1 0 0 0 0 6 (8) 6 5 1 (3) 0 (2) 1 0 0 0 0 0 0 0 (1) 0 0 (1) 0 1 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggtttcacctttagtaacgattgg atggactgggtccgccaggctccagggaaggggctggagtgggtggccaat ataaagggagatggaagtgagaaa aactatgtagactctgcgaagggccgattcatcatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgt tcgagactccttgaggaa SRLLEE IGHV3-7 IGHD7-27 IGHJ4 ggtttcacctttagtaacgattgg atggactgggtccgccaggctccagggaaggggctggagtgggtggccaat ataaagggagatggaagtgagaaa aactatgtagactctgcgaagggccgattcatcatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgt tcgagactccttgaggaa IGA1 13 213 9 4 6 7 3 6 2 1 6 4 JY8QFUQ01DJU2K 21989 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 800 86.05 185/215 nt 84.65 182/215 nt Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F 105 68.75 33/48 nt Homsap IGHD3-10*01 F 1 8 7 13 8.7.13 [1.17.38.11] CATVRHLGERIFDFW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'C' (1 nucleotides at position 12 to 12 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 45 of V-REGION: 'T' (1 nucleotides at position 49 to 49 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 63 of V-REGION: 'G' (1 nucleotides at position 96 to 96 in the user submitted sequence), (cause frameshift); tctctgggggcCaccatgagtagtttctactggagctgggttcggcagTcccccagggaggggactggagtggattggatttgtttcttacagtgGggcccaccaactacagcccctccctcaagagtcgagtcaacttatcactggacgcggccaacaaacagttctctttgcagctgcgttctgtgaccgctgcggacacggccatttattactgtgcgacagtacgacatctcggggaacgaatattcgatttctggggccagggtatcctggtcaccgtccccctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgccctggtccagggcttcttcccccc NA IGA1 93 95 [289] t81>g|t85>a,S29>T(+ - +)|c90>g,I30>M(+ + -)|a110>t,Y37>F(- + -)|a124>g,I42>V(+ - +)|c126>t,I42>V(+ - +)|a143>g,K48>R(+ + +)|g162>a|a164>t,Y55>F(- + -)|a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|a170>c,Y57>S(+ - -)|a190>c,S64>P(+ - -)|g191>c,S64>P(+ - -)|a203>g,N68>S(- - -)|c230>a,T77>N(- + -)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|a244>g,T82>A(- - -)|t247>g,S83>A(- + -)|g252>c,K84>N(+ - -)|c255>a,N85>K(+ - -)|c264>t|c265>t|a268>c,K90>Q(+ - -)|a274>c,S92>R(- - -)|c276>t,S92>R(- - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|g317>c,R106>T(- - -)| t81>g|t85>a,S29>T(+ - +)|c90>g,I30>M(+ + -)|a110>t,Y37>F(- + -)| a124>g,I42>V(+ - +)|c126>t,I42>V(+ - +)|a143>g,K48>R(+ + +)|g162>a|a164>t,Y55>F(- + -)| a166>g,I56>V(+ - +)|c168>t,I56>V(+ - +)|a170>c,Y57>S(+ - -)|a190>c,S64>P(+ - -)|g191>c,S64>P(+ - -)| a203>g,N68>S(- - -)|c230>a,T77>N(- + -)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|a240>g,V80>L(+ - +)|a244>g,T82>A(- - -)|t247>g,S83>A(- + -)|g252>c,K84>N(+ - -)|c255>a,N85>K(+ - -)|c264>t|c265>t|a268>c,K90>Q(+ - -)|a274>c,S92>R(- - -)|c276>t,S92>R(- - -)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| g317>c,R106>T(- - -)|a320>t| 319 (320) 222 (223) 191 31 (32) 4 (5) 27 6 2 5 0 4 2 3 (4) 1 4 1 1 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 0 0 0 0 1 1 0 1 0 1 51 51 46 5 1 4 2 1 1 0 0 0 1 0 0 0 0 0 30 21 16 5 0 5 1 0 1 0 2 0 0 0 1 0 0 0 117 114 98 16 2 14 3 1 3 0 2 2 1 0 2 0 1 1 7 (8) 7 6 1 (2) 0 (1) 1 0 0 0 0 0 0 0 (1) 0 1 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct gggggcaccatgagtagtttctac tggagctgggttcggcagcccccagggaggggactggagtggattggattt gtttcttacagtgggcccacc aactacagcccctccctcaagagtcgagtcaacttatcactggacgcggccaacaaacagttctctttgcagctgcgttctgtgaccgctgcggacacggccatttattactgt gcgacagtacgacatctcggggaacgaatattcgatttc ATVRHLGERIFDF IGHV4-59 IGHD3-10 IGHJ4 gggggcaccatgagtagtttctac tggagctgggttcggcagcccccagggaggggactggagtggattggattt gtttcttacagtgggcccacc aactacagcccctccctcaagagtcgagtcaacttatcactggacgcggccaacaaacagttctctttgcagctgcgttctgtgaccgctgcggacacggccatttattactgt gcgacagtacgacatctcggggaacgaatattcgatttc IGA1 30 210 13 17 7 14 6 16 3 1 18 8 JY8QFUQ01DKCDX 11117 productive Homsap IGHV3-7*01 F 918 91.71 199/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-3*02 F 2 8 8 14 8.8.14 [1.17.38.11] CARGSYWHYDTFGDYW in-frame + ctctggatttacttttaacaactattggatgacctgggttcgccaggctccagggaaggggctggaatgggtggccaacataaaacaacatggaggtgaaacgtactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgtgcgagagggtcgtattggcattatgataccttcggcgactattggggcccgggagtcctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccccctggcgccctgctccaggagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG3 93 100 [292, 291] c84>t|c87>t|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|c126>t|g153>a|g171>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| c84>t|c87>t|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|c126>t|g153>a| g171>a|g175>c,D59>H(- - -)|a187>g,S63>G(+ + -)|g192>a|a194>c,K65>T(- - -)|a195>g,K65>T(- - -)| a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|c259>g,L87>V(+ - +)|g303>c| a320>g| 319 (320) 224 (225) 206 18 (19) 7 (8) 11 3 (4) 5 3 1 2 0 0 0 3 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 0 2 2 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 0 1 0 0 0 30 24 18 6 2 4 2 2 0 0 1 0 0 0 1 0 0 0 117 114 110 4 1 3 1 0 0 0 1 0 0 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatttacttttaacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccaac ataaaacaacatggaggtgaaacg tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat ARGSYWHYDTFGDY IGHV3-7 IGHD3-3 IGHJ4 ggatttacttttaacaactattgg atgacctgggttcgccaggctccagggaaggggctggaatgggtggccaac ataaaacaacatggaggtgaaacg tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccgagacctcagtgtatctgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgagagggtcgtattggcattatgataccttcggcgactat IGG3 18 213 12 6 8 12 4 6 3 4 4 7 JY8QFUQ01DKKJ0 19003 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 797 85.32 186/218 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD5-18*01 F 2 8 8 16 8.8.16 [1.17.38.11] CAKVWIELWSAGDHFDNW in-frame + cctctggattcatatttactagatatgccatgacctgggtccgccaggctccagggaagggtctggagtgggtcgcttctatcagtggtagtgggattagtaaaaagtacgcagacggcgtggagggccgattcaccatctccagagacagttccgagagaacactgtatctacaaatgaacagcctgagagtcgaggacacggccacatattattgtgcgaaagtatggatagagttatggtcggctggggaccactttgacaactggggccagggaaccctggtcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] c86>t,T29>I(- - -)|c87>a,T29>I(- - -)|g104>c,S35>T(+ - +)|c105>t,S35>T(+ - +)|c108>a,S36>R(- - -)|g119>c,S40>T(+ - +)|g147>t|t160>g,S54>A(- + -)|a162>t,S54>A(- + -)|g163>t,A55>S(- + -)|t168>c|t186>g|g187>a,G63>I(- - -)|g188>t,G63>I(- - -)|c192>t|c194>a,T65>K(- - -)|t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|t208>g,S70>G(+ + -)|c209>g,S70>G(+ + -)|a214>g,K72>E(+ - -)|g225>a|a245>g,N82>S(- - -)|a250>g,K84>E(+ - -)|a254>g,N85>R(+ - -)|c255>a,N85>R(+ - -)|g258>a|g267>a|c287>t,A96>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|c309>t| c86>t,T29>I(- - -)|c87>a,T29>I(- - -)|g104>c,S35>T(+ - +)|c105>t,S35>T(+ - +)|c108>a,S36>R(- - -)| g119>c,S40>T(+ - +)|g147>t|t160>g,S54>A(- + -)|a162>t,S54>A(- + -)|g163>t,A55>S(- + -)| t168>c|t186>g|g187>a,G63>I(- - -)|g188>t,G63>I(- - -)|c192>t|c194>a,T65>K(- - -)| t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|t208>g,S70>G(+ + -)|c209>g,S70>G(+ + -)|a214>g,K72>E(+ - -)|g225>a|a245>g,N82>S(- - -)|a250>g,K84>E(+ - -)|a254>g,N85>R(+ - -)|c255>a,N85>R(+ - -)|g258>a|g267>a|c287>t,A96>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|c309>t| a320>t| 319 (320) 225 (226) 193 32 (33) 8 (9) 24 4 5 5 2 0 4 1 (2) 1 2 2 3 3 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 0 2 0 0 2 0 0 1 0 0 0 51 51 46 5 1 4 0 0 0 0 0 0 1 0 1 0 2 1 30 24 18 6 3 3 0 1 1 1 0 1 0 0 0 0 1 1 117 114 98 16 4 12 4 4 2 1 0 1 0 1 0 2 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcatatttactagatatgcc atgacctgggtccgccaggctccagggaagggtctggagtgggtcgcttct atcagtggtagtgggattagtaaa aagtacgcagacggcgtggagggccgattcaccatctccagagacagttccgagagaacactgtatctacaaatgaacagcctgagagtcgaggacacggccacatattattgt gcgaaagtatggatagagttatggtcggctggggaccactttgacaac AKVWIELWSAGDHFDN IGHV3-23 IGHD5-18 IGHJ4 ggattcatatttactagatatgcc atgacctgggtccgccaggctccagggaagggtctggagtgggtcgcttct atcagtggtagtgggattagtaaa aagtacgcagacggcgtggagggccgattcaccatctccagagacagttccgagagaacactgtatctacaaatgaacagcctgagagtcgaggacacggccacatattattgt gcgaaagtatggatagagttatggtcggctggggaccactttgacaac IGA1 32 213 16 16 10 21 6 11 5 3 16 8 JY8QFUQ01DLA1D 5903 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 1030 97.69 211/216 nt 97.22 210/216 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD6-25*01 F 1 8 8 13 8.8.13 [1.16.38.11] CARDLTGYSSERNYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 58 in the user submitted sequence), (cause frameshift) ctctggattcaccttcagtaactatagcatgaactgggtccgccaggctccagggaagggctggagtgggtctcatccattagtagtagtgctaggtacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgcgagacctgaccgggtatagcagcgaacgaaactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [286] g107>a,S36>N(- - -)|a184>g,S62>A(- + -)|g185>c,S62>A(- + -)|t189>g,S63>R(- - -)|a263>c,Y88>S(+ - -)|a316>c| g107>a,S36>N(- - -)| a184>g,S62>A(- + -)|g185>c,S62>A(- + -)|t189>g,S63>R(- - -)| a263>c,Y88>S(+ - -)| a316>c| 320 224 218 6 1 5 1 1 0 0 2 0 0 0 1 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 50 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 1 0 0 1 117 114 113 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 8 8 7 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtaactatagc atgaactgggtccgccaggctccagggaagggctggagtgggtctcatcc attagtagtagtgctaggtacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgcgagacctgaccgggtatagcagcgaacgaaactac ARDLTGYSSERNY IGHV3-21 IGHD6-25 IGHJ4 ggattcaccttcagtaactatagc atgaactgggtccgccaggctccagggaagggctggagtgggtctcatcc attagtagtagtgctaggtacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgcgagacctgaccgggtatagcagcgaacgaaactac IGA1 5 212 2 3 1 2 1 3 0 0 1 4 JY8QFUQ01DM6TL 7551 productive Homsap IGHV3-7*01 F 873 89.4 194/217 nt NA Homsap IGHJ3*01 F (see comment) 151 78 39/50 nt Homsap IGHD3-16*01 F 2 8 8 8 8.8.8 [1.17.38.11] CARATNADVW in-frame + Other possibilities: Homsap_IGHJ6*02 (highest number of consecutive identical nucleotides) ctctggattcacctttagggggtactggatgacctgggtccgccaggctccagggaaggggctggagtgggtggccaccataaatcacgatggaagtcaaatttactatatggaccttgtgaagggccggttcaccatctccagagacaacgccgagacctcactctatttgcaaatgaacaccctgagagccgaagacacggctgtctattactgtgcgagggcgactaatgccgacgtctggggccaagggaccacggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 100 100 [272] t105>g,S35>R(- - -)|a106>g,S36>G(+ + -)|c108>g,S36>G(+ + -)|t111>c|g119>c,S40>T(+ - +)|a164>c,N55>T(- + -)|g171>t,K57>N(+ - -)|a174>c,Q58>H(- + -)|g190>c,E64>Q(+ + -)|g192>a,E64>Q(+ + -)|a194>t,K65>I(- + -)|a195>t,K65>I(- + -)|g202>a,V68>M(+ - -)|t208>c,S70>L(- - -)|c209>t,S70>L(- - -)|a225>g|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|g261>c|c265>t|g278>c,S93>T(+ - +)|g291>a|g303>c| t105>g,S35>R(- - -)|a106>g,S36>G(+ + -)|c108>g,S36>G(+ + -)|t111>c| g119>c,S40>T(+ - +)|a164>c,N55>T(- + -)| g171>t,K57>N(+ - -)|a174>c,Q58>H(- + -)|g190>c,E64>Q(+ + -)|g192>a,E64>Q(+ + -)|a194>t,K65>I(- + -)|a195>t,K65>I(- + -)| g202>a,V68>M(+ - -)|t208>c,S70>L(- - -)|c209>t,S70>L(- - -)|a225>g|a250>g,K84>E(+ - -)|a254>c,N85>T(- + -)|g261>c|c265>t|g278>c,S93>T(+ - +)|g291>a|g303>c| a318>g|a320>c| 317 (320) 222 (225) 199 (200) 23 (25) 6 (8) 17 3 (4) 3 2 2 3 (4) 0 2 0 5 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 0 1 0 0 0 0 0 1 0 1 51 51 49 2 0 2 0 0 0 0 1 0 0 0 1 0 0 0 30 24 18 6 0 6 0 1 0 0 1 0 2 0 1 0 1 0 117 114 103 11 5 6 2 2 2 1 1 0 0 0 3 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagggggtactgg atgacctgggtccgccaggctccagggaaggggctggagtgggtggccacc ataaatcacgatggaagtcaaatt tactatatggaccttgtgaagggccggttcaccatctccagagacaacgccgagacctcactctatttgcaaatgaacaccctgagagccgaagacacggctgtctattactgt gcgagggcgactaatgccgacgtc ARATNADV IGHV3-7 IGHD3-16 IGHJ3 ggattcacctttagggggtactgg atgacctgggtccgccaggctccagggaaggggctggagtgggtggccacc ataaatcacgatggaagtcaaatt tactatatggaccttgtgaagggccggttcaccatctccagagacaacgccgagacctcactctatttgcaaatgaacaccctgagagccgaagacacggctgtctattactgt gcgagggcgactaatgccgacgtc IGA2 23 213 10 13 5 12 5 11 5 1 8 9 JY8QFUQ01DN21P 11830 productive Homsap IGHV3-48*02 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-22*01 F 2 8 8 14 8.8.14 [1.17.38.11] CAKDRGGSAYPIFDYW in-frame + ctctggattcaccttcggcagttataggatgagctgggtccgccaggctccagggaaggggctggagtggatttcatacattagtagtagtagtagtgccatctattacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcagtgtatctgcaaatgaacagcctgagagacgaggacacggctatatattactgtgcgaaagataggggcggtagtgcttatcccatctttgactactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatggaacgtggtcatcgcctgcctggtccagggcttcttcccc NA IGA1 100 89 [290, 289] a103>g,S35>G(+ + -)|t105>c,S35>G(+ + -)|c108>t|c114>g,S38>R(- - -)|a119>g,N40>S(- - -)|g157>a,V53>I(+ - +)|a190>g,T64>A(- - -)|a195>c|c198>t|c259>g,L87>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|g317>a,R106>K(+ + +)| a103>g,S35>G(+ + -)|t105>c,S35>G(+ + -)|c108>t|c114>g,S38>R(- - -)| a119>g,N40>S(- - -)|g157>a,V53>I(+ - +)| a190>g,T64>A(- - -)|a195>c| c198>t|c259>g,L87>V(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g317>a,R106>K(+ + +)| 320 225 212 13 3 10 3 4 2 1 1 0 0 0 0 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 1 1 0 0 0 0 0 1 0 0 51 51 49 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 30 24 22 2 1 1 1 0 0 0 1 0 0 0 0 0 0 0 117 114 110 4 1 3 0 2 1 0 0 0 0 0 0 1 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ctct ggattcaccttcggcagttatagg atgagctgggtccgccaggctccagggaaggggctggagtggatttcatac attagtagtagtagtagtgccatc tattacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcagtgtatctgcaaatgaacagcctgagagacgaggacacggctatatattactgt gcgaaagataggggcggtagtgcttatcccatctttgactac AKDRGGSAYPIFDY IGHV3-48 IGHD3-22 IGHJ4 ggattcaccttcggcagttatagg atgagctgggtccgccaggctccagggaaggggctggagtggatttcatac attagtagtagtagtagtgccatc tattacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcagtgtatctgcaaatgaacagcctgagagacgaggacacggctatatattactgt gcgaaagataggggcggtagtgcttatcccatctttgactac IGA1 12 213 9 3 5 7 4 5 1 2 5 4 JY8QFUQ01DNADY 22315 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 811 85.84 188/219 nt 85.39 187/219 nt Homsap IGHJ3*01 F 142 76 38/50 nt Homsap IGHD3-16*01 F 3 8 8 12 8.8.12 [2.17.38.11] CARDDGGHMTLDVW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 85 of V-REGION: 'GG' (2 nucleotides at position 160 to 161 in the user submitted sequence), (cause frameshift); atgtctggattcacatttagaagctattccatgaattgggtccgccaggctccagggaaggggctggagtgggtctcagctgtcagtggtggtgggggcgccacaaactacgcggagtccgtgaagggccggttcaccatctccagagacaattccaggGGggcacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgtgcgagagatgacggcggacacatgactttagatgtctggggccaagggacaatggtcagcgtgctctccggcctccaccaagggcccatcggtcttccccccgtggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggtgtcgtggaactcc NA IGG2 93 88 [292, 290] g73>a,A25>M(+ - -)|c74>t,A25>M(+ - -)|c75>g,A25>M(+ - -)|c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a175>g,S59>G(+ + -)|t186>g|t189>c|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|t196>a,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|a251>g,K84>R(+ + +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|c287>t,A96>V(+ - +)|g291>a|g297>a|g301>t,V101>L(+ - +)|c309>t|a317>g,K106>R(+ + +)| g73>a,A25>M(+ - -)|c74>t,A25>M(+ - -)|c75>g,A25>M(+ - -)| c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a175>g,S59>G(+ + -)|t186>g|t189>c|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| t196>a,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|a251>g,K84>R(+ + +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|c287>t,A96>V(+ - +)|g291>a|g297>a|g301>t,V101>L(+ - +)|c309>t| a317>g,K106>R(+ + +)| 320 227 195 32 9 23 8 5 5 3 0 2 1 1 1 3 2 1 78 6 3 3 0 3 0 1 1 0 0 0 0 0 0 1 0 0 36 24 21 3 1 2 0 0 0 0 0 2 0 0 0 0 1 0 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 24 17 7 2 5 3 0 0 2 0 0 0 0 1 0 0 1 117 114 98 16 6 10 4 3 3 1 0 0 1 1 0 2 1 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| atgtct ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagct gtcagtggtggtgggggcgccaca aactacgcggagtccgtgaagggccggttcaccatctccagagacaattccaggggcacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgt gcgagagatgacggcggacacatgactttagatgtc ARDDGGHMTLDV IGHV3-23 IGHD3-16 IGHJ3 ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagct gtcagtggtggtgggggcgccaca aactacgcggagtccgtgaagggccggttcaccatctccagagacaattccaggggcacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgt gcgagagatgacggcggacacatgactttagatgtc IGG2 28 213 18 10 8 15 10 13 6 3 12 7 JY8QFUQ01DO347 10989 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 945 93.09 202/217 nt NA Homsap IGHJ3*02 F 232 96 48/50 nt Homsap IGHD3-22*01 F 2 8 8 19 8.8.19 [1.17.38.11] CAKDREDSSGYYFFRDGFDIW in-frame + ctctggattcacgtttaggacctatgccatgacctgggtccgccaggctccagggaaggggctggagtgggtctccggtattggtggtagtggtcgaaccacacactacgcagactccgtgcagggccggttcaccatctccagagacaattccaagaacacggtggatctgcaaatgaacagcctgagagccgaggacacggccatatattactgtgcgaaagatcgtgaagatagtagtggttattacttcttccgtgatggttttgatatttggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 71 95 [305, 307] c87>g|c105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|g119>c,S40>T(+ - +)|a162>c|c164>g,A55>G(- + -)|a169>g,S57>G(+ + -)|g187>c,G63>R(- - -)|t189>a,G63>R(- - -)|g191>c,S64>T(+ - +)|t196>c,Y66>H(+ - -)|a214>c,K72>Q(+ - -)|c259>g,L87>V(+ - +)|t262>g,Y88>D(- - -)|g301>a,V101>I(+ - +)| c87>g|c105>g,S35>R(- - -)|g107>c,S36>T(+ - +)| g119>c,S40>T(+ - +)|a162>c|c164>g,A55>G(- + -)| a169>g,S57>G(+ + -)|g187>c,G63>R(- - -)|t189>a,G63>R(- - -)|g191>c,S64>T(+ - +)| t196>c,Y66>H(+ - -)|a214>c,K72>Q(+ - -)|c259>g,L87>V(+ - +)|t262>g,Y88>D(- - -)|g301>a,V101>I(+ - +)| 320 225 210 15 2 13 1 1 0 1 2 0 0 1 4 4 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 0 0 0 0 0 0 1 2 0 0 51 51 48 3 1 2 0 0 0 0 1 0 0 0 1 1 0 0 30 24 20 4 0 4 1 0 0 0 0 0 0 1 2 0 0 0 117 114 109 5 0 5 0 1 0 1 1 0 0 0 0 1 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgtttaggacctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctccggt attggtggtagtggtcgaaccaca cactacgcagactccgtgcagggccggttcaccatctccagagacaattccaagaacacggtggatctgcaaatgaacagcctgagagccgaggacacggccatatattactgt gcgaaagatcgtgaagatagtagtggttattacttcttccgtgatggttttgatatt AKDREDSSGYYFFRDGFDI IGHV3-23 IGHD3-22 IGHJ3 ggattcacgtttaggacctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctccggt attggtggtagtggtcgaaccaca cactacgcagactccgtgcagggccggttcaccatctccagagacaattccaagaacacggtggatctgcaaatgaacagcctgagagccgaggacacggccatatattactgt gcgaaagatcgtgaagatagtagtggttattacttcttccgtgatggttttgatatt IGA2 15 213 3 12 1 9 2 6 1 1 7 6 JY8QFUQ01DP55Y 7053 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 911 90.5 200/221 nt 89.59 198/221 nt Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 187 86 43/50 nt Homsap IGHD1-14*01 ORF 3 10 7 13 10.7.13 [1.17.38.11] CARHLQYNRHGFDFW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 64 to 64 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 89 of V-REGION: 'C' (1 nucleotides at position 175 to 175 in the user submitted sequence), (cause frameshift); tctctggtggctccgtcagcagtagtcattactactggggctggatccgccagaccccaggaaAaggggctggagtggcttgggacaatctattatagcggaagcgcctacatcaacccgtccctcaagagtcgagtcaccatttccgttgacatatctaagaaccagttctccCctgaggctgacctctgtgaccgccgcagacacggctgtctattactgtgcgagacacctgcaatataatcgacatggttttgatttctggggccaggggacaacggtcaccgtctcttcaacctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG1 100 100 [294] a88>g,I30>V(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|c133>a,P45>T(+ + -)|g141>a|a157>c,I53>L(+ + +)|g164>c,S55>T(+ - +)|t165>a,S55>T(+ - +)|t177>c|g189>a|a193>g,T65>A(- - -)|t199>a,Y67>I(- - -)|a200>t,Y67>I(- - -)|a234>t|a240>t|c245>t,T82>I(- - -)|g246>a,T82>I(- - -)|c249>t|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|g303>c| a88>g,I30>V(+ - +)|a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)| c133>a,P45>T(+ + -)|g141>a|a157>c,I53>L(+ + +)|g164>c,S55>T(+ - +)|t165>a,S55>T(+ - +)| t177>c|g189>a|a193>g,T65>A(- - -)| t199>a,Y67>I(- - -)|a200>t,Y67>I(- - -)|a234>t|a240>t|c245>t,T82>I(- - -)|g246>a,T82>I(- - -)|c249>t|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|g303>c| 320 229 208 21 7 14 3 4 2 1 2 1 3 2 3 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 1 1 0 0 1 0 0 0 0 0 0 0 51 51 46 5 1 4 0 1 0 0 1 1 0 1 1 0 0 0 30 21 18 3 2 1 1 1 0 1 0 0 0 0 0 0 0 0 117 114 104 10 4 6 1 1 2 0 0 0 3 1 2 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcagcagtagtcattactac tggggctggatccgccagaccccaggaaaggggctggagtggcttgggaca atctattatagcggaagcgcc tacatcaacccgtccctcaagagtcgagtcaccatttccgttgacatatctaagaaccagttctccctgaggctgacctctgtgaccgccgcagacacggctgtctattactgt gcgagacacctgcaatataatcgacatggttttgatttc ARHLQYNRHGFDF IGHV4-39 IGHD1-14 IGHJ3 ggtggctccgtcagcagtagtcattactac tggggctggatccgccagaccccaggaaaggggctggagtggcttgggaca atctattatagcggaagcgcc tacatcaacccgtccctcaagagtcgagtcaccatttccgttgacatatctaagaaccagttctccctgaggctgacctctgtgaccgccgcagacacggctgtctattactgt gcgagacacctgcaatataatcgacatggttttgatttc IGG1 21 216 10 11 6 10 4 11 5 2 10 4 JY8QFUQ01DP8YH 5080 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 882 89.86 195/217 nt 89.4 194/217 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD1-26*01 F 1 8 8 15 8.8.15 [1.17.38.11] CAKDQSCIVGPCGFDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 52 of V-REGION: 'G' (1 nucleotides at position 69 to 69 in the user submitted sequence), (cause frameshift); ctccgaattcatccttgacagttatgccatgagttgggtccgccaggccccagggaaggggctggagtGgggtctcggctattagtggaagtggtgcaaccacatactacgcagactccgtgaagggccggttcgccatctccagagacaattccaagaacacgctatatctacaaatgaacaacctaggggccgaggacacggccgtttattactgtgcgaaagatcagtcctgtatagtgggaccttgtggttttgactactggggccagggagccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA IGG2 86 100 [295] t78>c|g80>a,G27>E(- - -)|c86>t,T29>I(- - -)|t88>c,F30>L(+ - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c108>t|c120>t|t135>c|a162>g|t174>a|g188>c,G63>A(- + -)|t189>a,G63>A(- + -)|g191>c,S64>T(+ - +)|a229>g,T77>A(- - -)|g261>a|g267>a|g278>a,S93>N(- - -)|g282>a|a283>g,R95>G(- - -)|a285>g,R95>G(- - -)|a303>t| t78>c| g80>a,G27>E(- - -)|c86>t,T29>I(- - -)|t88>c,F30>L(+ - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c108>t| c120>t|t135>c|a162>g| t174>a|g188>c,G63>A(- + -)|t189>a,G63>A(- + -)|g191>c,S64>T(+ - +)| a229>g,T77>A(- - -)|g261>a|g267>a|g278>a,S93>N(- - -)|g282>a|a283>g,R95>G(- - -)|a285>g,R95>G(- - -)|a303>t| 320 225 203 22 10 12 5 6 3 3 0 0 1 2 2 0 0 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 18 6 1 5 1 2 2 1 0 0 0 0 0 0 0 0 51 51 48 3 3 0 1 0 1 1 0 0 0 0 0 0 0 0 30 24 20 4 1 3 0 0 0 0 0 0 0 2 2 0 0 0 117 114 106 8 4 4 3 4 0 0 0 0 1 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc gaattcatccttgacagttatgcc atgagttgggtccgccaggccccagggaaggggctggagtgggtctcggct attagtggaagtggtgcaaccaca tactacgcagactccgtgaagggccggttcgccatctccagagacaattccaagaacacgctatatctacaaatgaacaacctaggggccgaggacacggccgtttattactgt gcgaaagatcagtcctgtatagtgggaccttgtggttttgactac AKDQSCIVGPCGFDY IGHV3-23 IGHD1-26 IGHJ4 gaattcatccttgacagttatgcc atgagttgggtccgccaggccccagggaaggggctggagtgggtctcggct attagtggaagtggtgcaaccaca tactacgcagactccgtgaagggccggttcgccatctccagagacaattccaagaacacgctatatctacaaatgaacaacctaggggccgaggacacggccgtttattactgt gcgaaagatcagtcctgtatagtgggaccttgtggttttgactac IGG2 21 213 16 5 9 11 7 10 7 2 4 8 JY8QFUQ01DPFIO 11384 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 973 94.06 206/219 nt 93.15 204/219 nt Homsap IGHJ4*02 F 240 100 48/48 nt Homsap IGHD3-22*01 F 2 8 8 15 8.8.15 [2.17.38.11] CAKGDSGGYTHNYFDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 60 to 60 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 62 of V-REGION: 'A' (1 nucleotides at position 95 to 95 in the user submitted sequence), (cause frameshift); gcctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaAggggctggtgtgggcctcacgtgttaatggtgatAgggggtagcacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgcgacctgaggacacggccttgtattactgtgcaaaaggggatagtggtggttatacccacaactactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 100 [297] t158>c,V53>A(+ - +)|a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a256>t,T86>S(+ - +)|g258>c,T86>S(+ - +)|a283>c|g286>c,A96>P(- - -)|c288>t,A96>P(- - -)|t300>c|g301>t,V101>L(+ - +)|g317>a,R106>K(+ + +)| t158>c,V53>A(+ - +)| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a256>t,T86>S(+ - +)|g258>c,T86>S(+ - +)|a283>c|g286>c,A96>P(- - -)|c288>t,A96>P(- - -)|t300>c|g301>t,V101>L(+ - +)| g317>a,R106>K(+ + +)|a320>g| 319 (320) 226 (227) 212 14 (15) 2 (3) 12 4 (5) 1 1 2 1 0 1 0 3 0 1 0 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 30 24 21 3 0 3 3 0 0 0 0 0 0 0 0 0 0 0 117 114 105 9 2 7 1 0 1 1 1 0 1 0 3 0 1 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gcctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggcctcacgt gttaatggtgatgggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgcgacctgaggacacggccttgtattactgt gcaaaaggggatagtggtggttatacccacaactactttgactac AKGDSGGYTHNYFDY IGHV3-74 IGHD3-22 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggcctcacgt gttaatggtgatgggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaactccctgtatctgcaaatgaacagtctgcgacctgaggacacggccttgtattactgt gcaaaaggggatagtggtggttatacccacaactactttgactac IGA1 13 213 7 6 1 5 6 8 2 0 8 3 JY8QFUQ01DQV12 19625 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 941 92.66 202/218 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-2*01 F 3 8 8 11 8.8.11 [1.17.38.11] CARFGPLTRPDYW in-frame - cctctggattcacctttaccaactatggcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtggtgaaaccacataccacgcagaatccgtgcagggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgtgcgagattcggaccacttactcgacctgactactggggccagggaaccctggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG4 100 88 [283] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)|c164>g,A55>G(- + -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>c,S64>T(+ - +)|t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a214>c,K72>Q(+ - -)|t246>c|c257>a,T86>N(- + -)|g258>t,T86>N(- + -)|c279>t|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)| c164>g,A55>G(- + -)| g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>c,S64>T(+ - +)| t199>c,Y67>H(+ - -)|c207>a,D69>E(+ - +)|a214>c,K72>Q(+ - -)|t246>c|c257>a,T86>N(- + -)|g258>t,T86>N(- + -)|c279>t|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| a317>g,K106>R(+ + +)|g319>t|a320>t| 316 (320) 222 (226) 206 (207) 16 (19) 2 (4) 14 (15) 0 (1) 3 1 2 1 2 1 (2) 1 2 2 1 (2) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 21 3 0 3 0 1 0 0 0 0 0 1 1 0 0 0 117 114 105 9 2 7 0 1 1 2 1 2 1 0 0 0 1 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgaaaccaca taccacgcagaatccgtgcagggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgt gcgagattcggaccacttactcgacctgactac ARFGPLTRPDY IGHV3-23 IGHD2-2 IGHJ4 ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgaaaccaca taccacgcagaatccgtgcagggccggttcaccatctccagagacaactccaagaacaatctgtatctgcaaatgaacagtctgagagccgaggacacggccatttattactgt gcgagattcggaccacttactcgacctgactac IGG4 16 213 6 10 4 11 2 5 2 0 8 6 JY8QFUQ01DQVYX 13503 productive Homsap IGHV3-48*02 F 891 90.32 196/217 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 210 90.2 46/51 nt Homsap IGHD5-24*01 ORF 2 8 8 15 8.8.15 [1.17.38.11] CARGQKWLQVLRWFDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcaccttcgacagatacagtatgaactgggtccgccaggctccagggaggggactggagtggatttcatacataagtactactactagtaacagatactacgcagacgctgtgaagggccgattcaccatctccagagacaatgccaagaactcgctgtatttgcaaatggacagcctgagagacgaggacacggctgtatattattgtgcgagaggtcaaaaatggctacaagtgcttcgctggttcgactcctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA IGG2 100 100 [294] a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|t105>c,S35>D(- - -)|c108>a,S36>R(- - -)|t111>c|c114>t|a143>g,K48>R(+ + +)|g147>a|g157>a,V53>I(+ - +)|t168>a|g173>c,S58>T(+ - +)|g176>c,S59>T(+ - +)|g185>c,S62>T(+ - +)|c191>a,T64>N(- + -)|t194>g,I65>R(- + -)|t208>g,S70>A(- + -)|a258>g|c265>t|a274>g,N92>D(+ + -)|g303>a|c309>t| a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|t105>c,S35>D(- - -)|c108>a,S36>R(- - -)|t111>c|c114>t| a143>g,K48>R(+ + +)|g147>a|g157>a,V53>I(+ - +)| t168>a|g173>c,S58>T(+ - +)|g176>c,S59>T(+ - +)|g185>c,S62>T(+ - +)|c191>a,T64>N(- + -)|t194>g,I65>R(- + -)| t208>g,S70>A(- + -)|a258>g|c265>t|a274>g,N92>D(+ + -)|g303>a|c309>t| a320>g| 319 (320) 224 (225) 203 21 (22) 8 (9) 13 4 (5) 4 3 2 0 2 0 1 3 0 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 2 4 1 1 1 2 0 1 0 0 0 0 0 0 51 51 48 3 1 2 1 2 0 0 0 0 0 0 0 0 0 0 30 24 18 6 1 5 0 0 0 0 0 1 0 1 3 0 0 1 117 114 108 6 4 2 2 1 2 0 0 0 0 0 0 0 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ctct ggattcaccttcgacagatacagt atgaactgggtccgccaggctccagggaggggactggagtggatttcatac ataagtactactactagtaacaga tactacgcagacgctgtgaagggccgattcaccatctccagagacaatgccaagaactcgctgtatttgcaaatggacagcctgagagacgaggacacggctgtatattattgt gcgagaggtcaaaaatggctacaagtgcttcgctggttcgactcc ARGQKWLQVLRWFDS IGHV3-48 IGHD5-24 IGHJ5 ggattcaccttcgacagatacagt atgaactgggtccgccaggctccagggaggggactggagtggatttcatac ataagtactactactagtaacaga tactacgcagacgctgtgaagggccgattcaccatctccagagacaatgccaagaactcgctgtatttgcaaatggacagcctgagagacgaggacacggctgtatattattgt gcgagaggtcaaaaatggctacaagtgcttcgctggttcgactcc IGG2 21 213 13 8 7 12 6 9 5 3 4 9 JY8QFUQ01DRFXI 7276 productive (see comment) Homsap IGHV4-61*02 F 874 89.04 195/219 nt 88.58 194/219 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-24*01 ORF 3 10 7 13 10.7.13 [1.17.37.11] CARDFGGSYNLVAYW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT, from codon 68 of V-REGION: 1 nucleotides (from position 114 in the user submitted sequence), (cause frameshift) ctctggtgactccatcagtagtgattctcactactggagttggatccggcagcccgccgggaagggactggagtggattgggcgtgtctacgccagtgggaccaccaattacagccctccctcaagagtcgagtcaccatttcagtggacacgtccaggaatcaattctccctgaagttgaattctgtgaccgccgctgacacggccgtttatttctgtgcgagagattttggaggttcctacaatttagtggcctactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA IGG2 100 100 [290] g83>a,G28>D(- - -)|c93>t|g104>a,G35>D(- - -)|a106>t|g107>c|t109>c,Y37>H(+ - -)|c120>t|a166>g,I56>V(+ - +)|t171>c|a172>g,T58>A(- - -)|g191>c,S64>T(+ - +)|c198>t|c204>g|a234>t|a240>g|a251>g,K84>R(+ + +)|c255>t|g258>a|c271>t|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)|a291>t|g303>t|a308>t,Y103>F(- + -)| g83>a,G28>D(- - -)|c93>t|g104>a,G35>D(- - -)|a106>t|g107>c|t109>c,Y37>H(+ - -)| c120>t| a166>g,I56>V(+ - +)|t171>c|a172>g,T58>A(- - -)|g191>c,S64>T(+ - +)| c198>t|c204>g|a234>t|a240>g|a251>g,K84>R(+ + +)|c255>t|g258>a|c271>t|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)|a291>t|g303>t|a308>t,Y103>F(- + -)| 320 227 203 24 14 10 4 4 6 2 0 0 4 0 2 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 3 3 0 2 1 1 0 0 1 0 1 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 21 17 4 1 3 2 0 0 1 0 0 0 0 1 0 0 0 117 113 100 13 9 4 2 2 4 0 0 0 3 0 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtgactccatcagtagtgattctcactac tggagttggatccggcagcccgccgggaagggactggagtggattgggcgt gtctacgccagtgggaccacc aattacagccctccctcaagagtcgagtcaccatttcagtggacacgtccaggaatcaattctccctgaagttgaattctgtgaccgccgctgacacggccgtttatttctgt gcgagagattttggaggttcctacaatttagtggcctac ARDFGGSYNLVAY IGHV4-61 IGHD5-24 IGHJ4 ggtgactccatcagtagtgattctcactac tggagttggatccggcagcccgccgggaagggactggagtggattgggcgt gtctacgccagtgggaccacc aattacagccctccctcaagagtcgagtcaccatttcagtggacacgtccaggaatcaattctccctgaagttgaattctgtgaccgccgctgacacggccgtttatttctgt gcgagagattttggaggttcctacaatttagtggcctac IGG2 24 215 16 8 10 14 6 10 10 4 4 6 JY8QFUQ01DSBEO 12375 productive Homsap IGHV3-48*02 F, or Homsap IGHV3-48*04 F 810 86.18 187/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-21*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAKVGNLYFFEYW in-frame + ctctggattcagcttcaacagctacagcatgaactgggtccgccaggctccagggaagggactggaatggatctcatcaattagtaccgctggcaccaccataggctacgcagactctgtgaagggccgattcactatttccagagacaacgccaagaactcagtatctctgcagatggacagcctgagagacgaggacacggcggtatattactgtgccaaagtgggtaatttgtatttctttgaatactggggccggggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA IGA2 100 100 [281] c86>g,T29>S(+ - +)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|t111>c|g147>a|g153>a|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|a164>c,Y55>S(+ - -)|c165>a,Y55>S(+ - -)|g173>c,S58>T(+ - +)|t174>c,S58>T(+ - +)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|a184>g,S62>G(+ + -)|t186>c,S62>G(+ + -)|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)|t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c231>t|c234>t|t246>c|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a263>c,Y88>S(+ - -)|a270>g|a274>g,N92>D(+ + -)|t300>g|g303>a|g315>c|g317>a,R106>K(+ + +)| c86>g,T29>S(+ - +)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|t111>c| g147>a|g153>a|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|a164>c,Y55>S(+ - -)|c165>a,Y55>S(+ - -)| g173>c,S58>T(+ - +)|t174>c,S58>T(+ - +)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|a184>g,S62>G(+ + -)|t186>c,S62>G(+ + -)|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)| t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c231>t|c234>t|t246>c|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a263>c,Y88>S(+ - -)|a270>g|a274>g,N92>D(+ + -)|t300>g|g303>a| g315>c|g317>a,R106>K(+ + +)|a320>t| 319 (320) 224 (225) 192 32 (33) 10 (11) 22 5 7 2 7 2 1 0 (1) 0 4 2 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 0 2 0 0 0 0 0 1 0 0 51 51 45 6 2 4 0 3 0 1 1 1 0 0 0 0 0 0 30 24 16 8 0 8 2 0 0 3 0 0 0 0 3 0 0 0 117 114 102 12 6 6 3 2 2 1 1 0 0 0 0 1 0 2 7 (8) 7 5 2 (3) 1 (2) 1 0 1 0 0 0 0 0 (1) 0 1 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ctct ggattcagcttcaacagctacagc atgaactgggtccgccaggctccagggaagggactggaatggatctcatca attagtaccgctggcaccaccata ggctacgcagactctgtgaagggccgattcactatttccagagacaacgccaagaactcagtatctctgcagatggacagcctgagagacgaggacacggcggtatattactgt gccaaagtgggtaatttgtatttctttgaatac AKVGNLYFFEY IGHV3-48 IGHD2-21 IGHJ4 ggattcagcttcaacagctacagc atgaactgggtccgccaggctccagggaagggactggaatggatctcatca attagtaccgctggcaccaccata ggctacgcagactctgtgaagggccgattcactatttccagagacaacgccaagaactcagtatctctgcagatggacagcctgagagacgaggacacggcggtatattactgt gccaaagtgggtaatttgtatttctttgaatac IGA2 30 213 20 10 8 14 12 16 8 1 10 11 JY8QFUQ01DU1MH 10062 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 1034 98.14 211/215 nt NA Homsap IGHJ1*01 F 242 96.15 50/52 nt Homsap IGHD2-2*02 F 2 8 7 15 8.7.15 [1.17.38.11] CARGVGVSWHTEYFQHW in-frame + cctctgggttcaccgtcagtagcaactacatgagctgggtccgccaggctccagggaaggggctggagtgggtctcagttatttatagcggtggtaccacattctacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgcatcttcaaatgaacagcctgagagccgaggacacggccgtgtattactgtgcgagaggggtcggcgtcagctggcatactgaatacttccagcactggggccagggcaccctggtcatcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA IGA2 100 100 [291] g191>c,S64>T(+ - +)|a197>t,Y66>F(- + -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)| g191>c,S64>T(+ - +)| a197>t,Y66>F(- + -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)| a320>g| 319 (320) 222 (223) 218 4 (5) 0 (1) 4 0 (1) 0 0 1 0 0 1 0 1 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 20 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 117 114 111 3 0 3 0 0 0 1 0 0 1 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct gggttcaccgtcagtagcaactac atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagtt atttatagcggtggtaccaca ttctacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgcatcttcaaatgaacagcctgagagccgaggacacggccgtgtattactgt gcgagaggggtcggcgtcagctggcatactgaatacttccagcac ARGVGVSWHTEYFQH IGHV3-53 IGHD2-2 IGHJ1 gggttcaccgtcagtagcaactac atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagtt atttatagcggtggtaccaca ttctacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgcatcttcaaatgaacagcctgagagccgaggacacggccgtgtattactgt gcgagaggggtcggcgtcagctggcatactgaatacttccagcac IGA2 4 210 1 3 0 2 1 2 0 0 3 1 JY8QFUQ01DUKA9 4257 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 815 86.24 188/218 nt 85.78 187/218 nt Homsap IGHJ3*01 F 169 82 41/50 nt Homsap IGHD3-16*01 F 3 8 8 12 8.8.12 [1.17.38.11] CARDDGGHMTLDVW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 85 of V-REGION: 'G' (1 nucleotides at position 159 to 159 in the user submitted sequence), (cause frameshift); tgtctggattcacatttagaagctattccatgaattgggtccgccaggctccagggaaggggctggagtgggtctcagctgtcagtggtggtgggggcgccacaaactacgcggagtccgtgaagggccggttcaccatctccagagacaattccaggGggcacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgtgcgagagatgacggcggacacatgactttagatgtctggggccaagggacaatggtcagcgtctctccggcctccaccaagggcccatcggtcttcccccgtggcaccctcctccaagagcacctctgggggccacagcggccctgggctncctggtcaaggactcanttccccgaaccggtgacggtcgtgcgtggaactcag NA IGG1 79 81 [287, 288] c74>t|c75>g|c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a175>g,S59>G(+ + -)|t186>g|t189>c|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|t196>a,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|a251>g,K84>R(+ + +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|c287>t,A96>V(+ - +)|g291>a|g297>a|g301>t,V101>L(+ - +)|c309>t|a317>g,K106>R(+ + +)| c74>t|c75>g| c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|a175>g,S59>G(+ + -)|t186>g|t189>c|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| t196>a,Y66>N(- - -)|a204>g|c207>g,D69>E(+ - +)|a251>g,K84>R(+ + +)|a253>g,N85>G(- - -)|a254>g,N85>G(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|c265>t|g267>a|c287>t,A96>V(+ - +)|g291>a|g297>a|g301>t,V101>L(+ - +)|c309>t| a317>g,K106>R(+ + +)| 320 226 195 31 11 20 8 4 5 3 0 2 1 1 1 3 2 1 78 5 3 2 2 0 0 0 1 0 0 0 0 0 0 1 0 0 36 24 21 3 1 2 0 0 0 0 0 2 0 0 0 0 1 0 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 24 17 7 2 5 3 0 0 2 0 0 0 0 1 0 0 1 117 114 98 16 6 10 4 3 3 1 0 0 1 1 0 2 1 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tgtct ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagct gtcagtggtggtgggggcgccaca aactacgcggagtccgtgaagggccggttcaccatctccagagacaattccaggggcacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgt gcgagagatgacggcggacacatgactttagatgtc ARDDGGHMTLDV IGHV3-23 IGHD3-16 IGHJ3 ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagct gtcagtggtggtgggggcgccaca aactacgcggagtccgtgaagggccggttcaccatctccagagacaattccaggggcacggtgttcttacaaatgaacagcctgagagtcgaagacacagccttatattattgt gcgagagatgacggcggacacatgactttagatgtc IGG1 28 213 18 10 8 15 10 13 6 3 12 7 JY8QFUQ01DVTFY 1887 productive Homsap IGHV3-48*01 F, or Homsap IGHV3-48*02 F or Homsap IGHV3-48*04 F 864 88.94 193/217 nt NA Homsap IGHJ6*02 F (see comment) 188 78.69 48/61 nt Homsap IGHD3-16*01 F 3 8 8 12 8.8.12 [1.17.38.11] CAREGGRGYDMDVW in-frame + Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) ctctggattcaccttcagtatctatgccatgacctgggtccgccaggctccagggaaggggctggagtggatttcatttatcactgataggggtagtacccaatactacgcagactctgtgaagggccgattcaccgtctccagggaccaagccaagaactcactgtatctacaaatgaacaacctgggagtcgaggacacggctgtgtattattgtgcgagagaggggggacgtggctacgatatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagcccaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA IGA1 100 95 [283, 284] g107>t,S36>I(- - -)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)|a119>c,N40>T(- + -)|g157>a,V53>I(+ - +)|a164>t,Y55>F(- + -)|c165>t,Y55>F(- + -)|t168>c|g170>c,S57>T(+ - +)|a172>g,S58>D(- - -)|g173>a,S58>D(- - -)|t177>g,S59>R(- - -)|a184>g,S62>G(+ + -)|a193>c,I65>Q(- - -)|t194>a,I65>Q(- - -)|a232>g,I78>V(+ - +)|a240>g|a244>c,N82>Q(+ - +)|t246>a,N82>Q(+ - +)|g267>a|g278>a,S93>N(- - -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|c309>t| g107>t,S36>I(- - -)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)| a119>c,N40>T(- + -)|g157>a,V53>I(+ - +)|a164>t,Y55>F(- + -)|c165>t,Y55>F(- + -)| t168>c|g170>c,S57>T(+ - +)|a172>g,S58>D(- - -)|g173>a,S58>D(- - -)|t177>g,S59>R(- - -)|a184>g,S62>G(+ + -)|a193>c,I65>Q(- - -)|t194>a,I65>Q(- - -)| a232>g,I78>V(+ - +)|a240>g|a244>c,N82>Q(+ - +)|t246>a,N82>Q(+ - +)|g267>a|g278>a,S93>N(- - -)|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)|c309>t| 320 225 201 24 4 20 6 4 3 1 3 0 1 2 2 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 0 0 0 0 0 0 0 1 0 1 0 51 51 47 4 0 4 0 1 1 0 1 0 1 0 0 0 0 0 30 24 16 8 1 7 2 1 0 1 1 0 0 1 1 0 0 1 117 114 105 9 3 6 3 2 2 0 1 0 0 1 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtatctatgcc atgacctgggtccgccaggctccagggaaggggctggagtggatttcattt atcactgataggggtagtacccaa tactacgcagactctgtgaagggccgattcaccgtctccagggaccaagccaagaactcactgtatctacaaatgaacaacctgggagtcgaggacacggctgtgtattattgt gcgagagaggggggacgtggctacgatatggacgtc AREGGRGYDMDV IGHV3-48 IGHD3-16 IGHJ6 ggattcaccttcagtatctatgcc atgacctgggtccgccaggctccagggaaggggctggagtggatttcattt atcactgataggggtagtacccaa tactacgcagactctgtgaagggccgattcaccgtctccagggaccaagccaagaactcactgtatctacaaatgaacaacctgggagtcgaggacacggctgtgtattattgt gcgagagaggggggacgtggctacgatatggacgtc IGA1 24 213 14 10 7 10 7 14 3 1 10 10 JY8QFUQ01A0JN8 19999 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 887 89.91 196/218 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-9*01 F 2 8 8 10 8.8.10 [1.17.38.11] CVSDLAGQKGSW in-frame + cctcaggattcaccttcagtaggtactggatgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgtattaatgaagacggcagccacacagatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagttgtatttgcaaatgaacagtctgagaggcgaggacacggctgtctattattgtgtaagcgatttggctggtcagaaaggttcgtggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [279, 280] t78>a|c108>g,S36>R(- - -)|c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|c259>t|c265>t|c287>g,A96>G(- + -)|g303>c|c309>t|c314>t,A105>V(+ - +)|a318>c,R106>S(- - -)| t78>a| c108>g,S36>R(- - -)| c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|c259>t|c265>t|c287>g,A96>G(- + -)|g303>c|c309>t| c314>t,A105>V(+ - +)|a318>c,R106>S(- - -)| 320 226 202 24 8 16 2 3 6 4 2 1 0 2 2 2 0 0 78 5 4 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 49 2 0 2 0 0 1 1 0 0 0 0 0 0 0 0 30 24 16 8 3 5 1 2 0 2 1 0 0 1 1 0 0 0 117 114 104 10 4 6 1 1 4 1 0 1 0 0 1 1 0 0 8 8 6 2 0 2 0 0 1 0 1 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctca ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagccacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagttgtatttgcaaatgaacagtctgagaggcgaggacacggctgtctattattgt gtaagcgatttggctggtcagaaaggttcg VSDLAGQKGS IGHV3-74 IGHD3-9 IGHJ4 ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagccacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagttgtatttgcaaatgaacagtctgagaggcgaggacacggctgtctattattgt gtaagcgatttggctggtcagaaaggttcg unmatched 21 213 14 7 8 13 6 8 4 3 8 6 JY8QFUQ01A0KG1 7579 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-10*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAIEHTLPSGQGLGIEYFW in-frame + ctctggattcaactttaacagctttgccatgagctgggtccgccaggttccagggatggggctggagtgggtctcagccattagtggtagtggcgggagcacattctacgcagactccgtgaagggccggttcaccatctccagagacaactccaacaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccttatattattgtgcgatcgagcatacccttccgtccggacagggattagggattgaatatttctggggccagggaaccctggtcatcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccgaagatgggaacgtggtcgtcgcctgcctggtccagggcttcttccccca NA unmatched, IGA2 57 95 [299, 301] c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|a110>t,Y37>F(- + -)|c134>t,A45>V(+ - +)|a143>t,K48>M(- + -)|t165>c|t186>c|t189>g|a197>t,Y66>F(- + -)|t246>c|g252>c,K84>N(+ - -)|g301>t,V101>L(+ - +)|c309>t| c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|a110>t,Y37>F(- + -)| c134>t,A45>V(+ - +)|a143>t,K48>M(- + -)|t165>c| t186>c|t189>g| a197>t,Y66>F(- + -)|t246>c|g252>c,K84>N(+ - -)|g301>t,V101>L(+ - +)|c309>t| a317>t,K106>I(- + -)|a318>c,K106>I(- + -)| 316 (320) 221 (225) 208 (210) 13 (15) 5 8 (10) 0 1 2 3 0 (1) 1 3 (4) 0 1 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 1 1 0 0 0 0 0 51 51 48 3 1 2 0 0 1 1 0 0 1 0 0 0 0 0 30 24 22 2 2 0 0 0 0 1 0 0 0 0 0 0 0 1 117 114 109 5 2 3 0 0 1 1 0 0 1 0 1 0 1 0 4 (8) 4 4 (6) 0 (2) 0 0 (2) 0 0 0 0 0 (1) 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaactttaacagctttgcc atgagctgggtccgccaggttccagggatggggctggagtgggtctcagcc attagtggtagtggcgggagcaca ttctacgcagactccgtgaagggccggttcaccatctccagagacaactccaacaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccttatattattgt gcgatcgagcatacccttccgtccggacagggattagggattgaatatttc AIEHTLPSGQGLGIEYF IGHV3-23 IGHD3-10 IGHJ4 ggattcaactttaacagctttgcc atgagctgggtccgccaggttccagggatggggctggagtgggtctcagcc attagtggtagtggcgggagcaca ttctacgcagactccgtgaagggccggttcaccatctccagagacaactccaacaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccttatattattgt gcgatcgagcatacccttccgtccggacagggattagggattgaatatttc unmatched 13 213 6 7 3 6 3 7 3 2 5 3 JY8QFUQ01A0XAA 14657 productive Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F 840 88.32 189/214 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-16*02 F 1 8 7 13 8.7.13 [1.17.38.11] CTRGRSDAWELSDHW in-frame + cgacgatgggtcctgcagaaactgcttctggagttggatccgccagtccccagggaaggggctggagtggattggggaggtcaatgatagaggaggcatcgactacaacccgtccctcaagagtcgagtcaccatatcattagacacgtccaacaaccaagtctccctgaggttgagctctgtgaccgccgcggacacggctgtgtattactgtacgcgagggcgatccgacgcgtgggaattatcggaccactggggccagggaaccgtggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA unmatched, IGA2 57 89 [284] t76>g,Y26>D(- - -)|t78>c,Y26>D(- - -)|g80>a,G27>D(- - -)|t89>g,F30>C(+ - -)|t105>a,S35>R(- - -)|g106>a,G36>N(- - -)|g107>a,G36>N(- - -)|t108>c,G36>N(- - -)|a110>g,Y37>C(- - -)|a113>t,Y38>F(- + -)|c120>t|c133>t,P45>S(+ - -)|a165>g|a166>g,I56>V(+ - +)|c172>g,H58>D(- - -)|t177>a,S59>R(- - -)|a190>g,S64>G(+ + -)|c194>t,T65>I(- - -)|a196>g,N66>D(+ + -)|g238>t,V80>L(+ - +)|g252>c,K84>N(+ - -)|g258>a|t259>g,F87>V(+ - -)|a269>g,K90>R(+ + +)|c271>t|g313>a,A105>T(- - -)|a316>c| t76>g,Y26>D(- - -)|t78>c,Y26>D(- - -)| g80>a,G27>D(- - -)|t89>g,F30>C(+ - -)|t105>a,S35>R(- - -)|g106>a,G36>N(- - -)|g107>a,G36>N(- - -)|t108>c,G36>N(- - -)|a110>g,Y37>C(- - -)|a113>t,Y38>F(- + -)| c120>t|c133>t,P45>S(+ - -)|a165>g| a166>g,I56>V(+ - +)|c172>g,H58>D(- - -)|t177>a,S59>R(- - -)|a190>g,S64>G(+ + -)|c194>t,T65>I(- - -)| a196>g,N66>D(+ + -)|g238>t,V80>L(+ - +)|g252>c,K84>N(+ - -)|g258>a|t259>g,F87>V(+ - -)|a269>g,K90>R(+ + +)|c271>t| g313>a,A105>T(- - -)|a316>c| 320 222 195 27 5 22 6 5 4 2 1 0 1 2 1 1 1 3 78 4 2 2 0 2 0 0 0 1 0 0 0 0 0 0 0 1 36 24 16 8 0 8 1 3 0 1 0 0 1 1 0 0 0 1 51 51 48 3 2 1 1 0 2 0 0 0 0 0 0 0 0 0 30 21 16 5 0 5 2 0 1 0 0 0 0 1 0 1 0 0 117 114 107 7 2 5 2 1 1 0 0 0 0 0 1 0 1 1 8 8 6 2 1 1 0 1 0 0 1 0 0 0 0 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| cgac gatgggtcctgcagaaactgcttc tggagttggatccgccagtccccagggaaggggctggagtggattggggag gtcaatgatagaggaggcatc gactacaacccgtccctcaagagtcgagtcaccatatcattagacacgtccaacaaccaagtctccctgaggttgagctctgtgaccgccgcggacacggctgtgtattactgt acgcgagggcgatccgacgcgtgggaattatcggaccac TRGRSDAWELSDH IGHV4-34 IGHD3-16 IGHJ4 gatgggtcctgcagaaactgcttc tggagttggatccgccagtccccagggaaggggctggagtggattggggag gtcaatgatagaggaggcatc gactacaacccgtccctcaagagtcgagtcaccatatcattagacacgtccaacaaccaagtctccctgaggttgagctctgtgaccgccgcggacacggctgtgtattactgt acgcgagggcgatccgacgcgtgggaattatcggaccac unmatched 23 210 15 8 8 11 7 12 4 0 6 13 JY8QFUQ01A1201 20086 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 995 95.41 208/218 nt NA Homsap IGHJ3*02 F 232 96 48/50 nt Homsap IGHD2-21*02 F 2 8 8 15 8.8.15 [1.17.38.11] CARLCGNDCRNDVFDIW in-frame + cctctggattcaccttcagtacctactggatgcactgggtccgccaagttccagggaaggggctggtgtgggtctcacgtgttgatagtgatgggactagcacagtctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctatatctgcaaatgaacagtctgaaagccgaggacacggctgtatattactgtgcaagattatgtggtaatgactgccgcaatgatgtttttgatatctggggccaagggacattggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccc NA unmatched, IGA2 57 89 [294, 295] g107>c,S36>T(+ - +)|c134>t,A45>V(+ - +)|a166>g,I56>V(+ - +)|a169>g,N57>D(+ + -)|g188>c,S63>T(+ - +)|a196>g,S66>V(- - -)|g197>t,S66>V(- - -)|g261>a|g284>a,R95>K(+ + +)|g303>a| g107>c,S36>T(+ - +)| c134>t,A45>V(+ - +)| a166>g,I56>V(+ - +)|a169>g,N57>D(+ + -)|g188>c,S63>T(+ - +)| a196>g,S66>V(- - -)|g197>t,S66>V(- - -)|g261>a|g284>a,R95>K(+ + +)|g303>a| g319>t|a320>t| 318 (320) 224 (226) 214 10 (12) 2 (4) 8 3 3 1 0 0 0 0 (1) 0 2 0 1 (2) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 2 0 0 0 0 0 0 0 1 0 0 0 117 114 109 5 2 3 1 3 0 0 0 0 0 0 0 0 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtacctactgg atgcactgggtccgccaagttccagggaaggggctggtgtgggtctcacgt gttgatagtgatgggactagcaca gtctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctatatctgcaaatgaacagtctgaaagccgaggacacggctgtatattactgt gcaagattatgtggtaatgactgccgcaatgatgtttttgatatc ARLCGNDCRNDVFDI IGHV3-74 IGHD2-21 IGHJ3 ggattcaccttcagtacctactgg atgcactgggtccgccaagttccagggaaggggctggtgtgggtctcacgt gttgatagtgatgggactagcaca gtctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctatatctgcaaatgaacagtctgaaagccgaggacacggctgtatattactgt gcaagattatgtggtaatgactgccgcaatgatgtttttgatatc unmatched 10 213 7 3 4 7 3 3 2 0 4 4 JY8QFUQ01A1J69 10647 productive Homsap IGHV3-7*01 F 954 93.55 203/217 nt NA Homsap IGHJ5*02 F (see comment) 183 84.31 43/51 nt Homsap IGHD3-22*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSDFYSTGHW in-frame + Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctggattcaacttcagaacctattggatgggctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagcacgatggaagtgacaagtactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcattgtttttgcaaatggacagcctgagagccgaggacacggctgtgtactactgtgcgattagcagtgatttctactcgacgggccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [281, 282] c86>a,T29>N(- + -)|t90>c|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|a118>g,S40>G(+ + -)|a174>c,Q58>H(- + -)|g192>c,E64>D(+ - +)|a195>g|a232>g,I78>V(+ - +)|c259>t|a263>t,Y88>F(- + -)|c265>t|a274>g,N92>D(+ + -)|t306>c| c86>a,T29>N(- + -)|t90>c|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)| a118>g,S40>G(+ + -)| a174>c,Q58>H(- + -)|g192>c,E64>D(+ - +)|a195>g| a232>g,I78>V(+ - +)|c259>t|a263>t,Y88>F(- + -)|c265>t|a274>g,N92>D(+ + -)|t306>c| g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 316 (320) 221 (225) 207 14 (18) 5 (7) 9 (11) 4 (5) 0 (1) 2 2 1 1 1 (2) 1 2 0 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 0 1 0 1 0 1 1 0 0 0 51 51 50 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 1 0 0 0 1 0 0 0 1 0 0 0 117 114 108 6 3 3 2 0 2 1 0 0 1 0 0 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaacttcagaacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcacgatggaagtgacaag tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcattgtttttgcaaatggacagcctgagagccgaggacacggctgtgtactactgt gcgattagcagtgatttctactcgacgggccac AISSDFYSTGH IGHV3-7 IGHD3-22 IGHJ5 ggattcaacttcagaacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcacgatggaagtgacaag tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcattgtttttgcaaatggacagcctgagagccgaggacacggctgtgtactactgt gcgattagcagtgatttctactcgacgggccac unmatched 14 213 8 6 2 5 6 9 3 2 4 5 JY8QFUQ01A2Y02 7481 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 821 85.97 190/221 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 192 86.27 44/51 nt Homsap IGHD3-22*01 F 2 10 7 13 10.7.13 [1.17.38.11] CARQLSGDYASFDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) tctctggtgaccccatcggcaacactgcttactcctggggctggatccgccagcccccagggaaggggctggagtggatcgcgactgtacattatgctggcagcacctactacaacccgtccctcaggagtcgagtcaccatctctgtggacacgtccaagaatgagttttccctgacactgacctctatgaccgccgcagacacggctgtatatttctgtgcgagacagctcagtggtgactacgcatcgtttgactcctggggccagggaaccctggtcgccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA unmatched, IGA2 57 89 [291] g83>a,G28>D(- - -)|t85>c,S29>P(+ - -)|a91>g,S31>G(+ + -)|g101>a,S34>N(- - -)|t102>c,S34>N(- - -)|g104>c,S35>T(+ - +)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a113>c,Y38>S(+ - -)|t159>c|g161>c,G54>A(- + -)|g164>c,S55>T(+ - +)|a166>g,I56>V(+ - +)|c168>a,I56>V(+ - +)|t169>c,Y57>H(+ - -)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|g189>c|a215>g,K72>R(+ + +)|a234>c|c237>t|a240>g|c255>t|c256>g,Q86>E(+ + -)|c261>t|a269>c,K90>T(- - -)|g270>a,K90>T(- - -)|g275>c,S92>T(+ - +)|g280>a,V94>M(+ - -)|g303>a|a308>t,Y103>F(- + -)| g83>a,G28>D(- - -)|t85>c,S29>P(+ - -)|a91>g,S31>G(+ + -)|g101>a,S34>N(- - -)|t102>c,S34>N(- - -)|g104>c,S35>T(+ - +)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a113>c,Y38>S(+ - -)| t159>c|g161>c,G54>A(- + -)|g164>c,S55>T(+ - +)| a166>g,I56>V(+ - +)|c168>a,I56>V(+ - +)|t169>c,Y57>H(+ - -)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|g189>c| a215>g,K72>R(+ + +)|a234>c|c237>t|a240>g|c255>t|c256>g,Q86>E(+ + -)|c261>t|a269>c,K90>T(- - -)|g270>a,K90>T(- - -)|g275>c,S92>T(+ - +)|g280>a,V94>M(+ - -)|g303>a|a308>t,Y103>F(- + -)| 320 229 198 31 8 23 6 5 3 4 3 1 1 0 7 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 21 9 0 9 2 2 0 2 1 0 0 0 2 0 0 0 51 51 48 3 1 2 0 0 0 1 0 0 0 0 2 0 0 0 30 21 15 6 1 5 2 0 0 1 0 1 0 0 2 0 0 0 117 114 101 13 6 7 2 3 3 0 2 0 1 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtgaccccatcggcaacactgcttactcc tggggctggatccgccagcccccagggaaggggctggagtggatcgcgact gtacattatgctggcagcacc tactacaacccgtccctcaggagtcgagtcaccatctctgtggacacgtccaagaatgagttttccctgacactgacctctatgaccgccgcagacacggctgtatatttctgt gcgagacagctcagtggtgactacgcatcgtttgactcc ARQLSGDYASFDS IGHV4-39 IGHD3-22 IGHJ5 ggtgaccccatcggcaacactgcttactcc tggggctggatccgccagcccccagggaaggggctggagtggatcgcgact gtacattatgctggcagcacc tactacaacccgtccctcaggagtcgagtcaccatctctgtggacacgtccaagaatgagttttccctgacactgacctctatgaccgccgcagacacggctgtatatttctgt gcgagacagctcagtggtgactacgcatcgtttgactcc unmatched 31 216 18 13 8 17 10 14 7 1 9 14 JY8QFUQ01A2ZKE 21998 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 842 87.61 191/218 nt NA Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD6-19*01 F 1 8 8 17 8.8.17 [1.17.38.11] CARDSASSGYARYDAMDVW in-frame + cctctggattcagttttagtacacatggcatgaactgggtccgccaggctccagggaaggggccggaatgggtctcattcgttaatagtggaagtagttacatctactacgcagactcagtgaggggccgattcaccatctccagagacgacgccaggaattcactgtatctgcaaatgcaccgcctgcgagtcgaggacacggctctctactattgtgcgagagacagtgcctcgtccggctacgctaggtacgacgctatggacgtctggggccaagggaccgcggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccccctggcgccctgctccaggagcacctccgaaagcacagcggccctgggctgcctggtcaaggactacttcccccgaaccggtgacggctgtcgtggaact NA unmatched, IGG2 64 88 [301, 302] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>t|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|t109>c,Y37>H(+ - -)|a112>g,S38>G(+ + -)|t149>c,L50>P(- - -)|g153>a|c164>t,S55>F(- - -)|a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|a195>c|a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|a251>g,K84>R(+ + +)|c255>t|a274>c,N92>H(- - -)|a277>c,S93>R(- - -)|a283>c|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t306>c|c309>t| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>t|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|t109>c,Y37>H(+ - -)|a112>g,S38>G(+ + -)| t149>c,L50>P(- - -)|g153>a|c164>t,S55>F(- - -)| a166>g,I56>V(+ - +)|g170>a,S57>N(- - -)|a175>g,S59>G(+ + -)|t177>a,S59>G(+ + -)|a195>c| a215>g,K72>R(+ + +)|a244>g,N82>D(+ + -)|a251>g,K84>R(+ + +)|c255>t|a274>c,N92>H(- - -)|a277>c,S93>R(- - -)|a283>c|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)|t306>c|c309>t| 320 226 199 27 7 20 6 2 6 3 4 1 0 1 3 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 1 6 1 0 2 1 0 1 0 0 1 1 0 0 51 51 48 3 1 2 0 1 1 1 0 0 0 0 0 0 0 0 30 24 19 5 1 4 2 1 0 0 1 0 0 1 0 0 0 0 117 114 102 12 4 8 3 0 3 1 3 0 0 0 2 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcagttttagtacacatggc atgaactgggtccgccaggctccagggaaggggccggaatgggtctcattc gttaatagtggaagtagttacatc tactacgcagactcagtgaggggccgattcaccatctccagagacgacgccaggaattcactgtatctgcaaatgcaccgcctgcgagtcgaggacacggctctctactattgt gcgagagacagtgcctcgtccggctacgctaggtacgacgctatggacgtc ARDSASSGYARYDAMDV IGHV3-21 IGHD6-19 IGHJ6 ggattcagttttagtacacatggc atgaactgggtccgccaggctccagggaaggggccggaatgggtctcattc gttaatagtggaagtagttacatc tactacgcagactcagtgaggggccgattcaccatctccagagacgacgccaggaattcactgtatctgcaaatgcaccgcctgcgagtcgaggacacggctctctactattgt gcgagagacagtgcctcgtccggctacgctaggtacgacgctatggacgtc unmatched 27 213 17 10 8 13 9 14 5 2 10 10 JY8QFUQ01A36OR 8840 productive Homsap IGHV3-21*04 F 990 95.39 207/217 nt NA Homsap IGHJ6*02 F, or Homsap IGHJ6*03 F 172 78.57 44/56 nt Homsap IGHD6-13*01 F 3 8 8 10 8.8.10 [1.17.38.11] CARAVSNQVDVW in-frame + ctctggattcgccttcagtacatatatcatgaattgggtccgccaggctccagggaaggggctggagtgggtctcatccattagttttagtagtgattacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgacgacacggccgtgtattactgtgcgagagccgtttcgaatcaggtggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [278, 279] a85>g,T29>A(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|g113>t,S38>I(- - -)|c120>t|a172>t,S58>F(- - -)|g173>t,S58>F(- - -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|g291>c,E97>D(+ - +)| a85>g,T29>A(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|g113>t,S38>I(- - -)| c120>t| a172>t,S58>F(- - -)|g173>t,S58>F(- - -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)| g291>c,E97>D(+ - +)| a320>c| 319 (320) 224 (225) 214 10 (11) 1 (2) 9 2 1 1 0 0 (1) 1 1 0 2 0 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 0 0 0 0 1 0 0 1 0 1 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 1 0 0 0 0 1 0 0 0 1 0 117 114 113 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcgccttcagtacatatatc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagttttagtagtgattacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgacgacacggccgtgtattactgt gcgagagccgtttcgaatcaggtggacgtc ARAVSNQVDV IGHV3-21 IGHD6-13 IGHJ6 ggattcgccttcagtacatatatc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagttttagtagtgattacata tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgacgacacggccgtgtattactgt gcgagagccgtttcgaatcaggtggacgtc unmatched 10 213 4 6 2 7 2 3 1 0 1 8 JY8QFUQ01A3PZ4 10071 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 936 92.63 201/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-10*01 F 2 8 8 13 8.8.13 [1.17.38.11] CANWLSGTHSKFDYW in-frame + ctctggattcaccttcagcaagtatgccatgagctgggtccgccaggctccaggggaggggctgcagtgggtctcagcaattagtggaaatggtgctgatatatactacgcagactccgtgaacggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgtgcgaattggctttcggggacacattctaagtttgactactggggcaagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [287, 288] t90>c|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a142>g,K48>E(+ - -)|g151>c,E51>Q(+ + -)|t165>a|t174>a|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c192>t,S64>D(- - -)|c194>t,T65>I(- - -)|g216>c,K72>N(+ - -)|g261>a|t306>c| t90>c|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)| a142>g,K48>E(+ - -)|g151>c,E51>Q(+ + -)|t165>a| t174>a|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c192>t,S64>D(- - -)|c194>t,T65>I(- - -)| g216>c,K72>N(+ - -)|g261>a|t306>c| a318>t,K106>N(+ - -)|g319>t|a320>g| 317 (320) 222 (225) 206 16 (19) 5 (7) 11 (12) 2 (3) 4 2 2 0 0 0 (1) 2 3 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 0 1 0 0 0 0 0 1 0 0 51 51 48 3 1 2 1 0 0 0 0 0 0 1 1 0 0 0 30 24 17 7 1 6 1 2 2 0 0 0 0 1 1 0 0 0 117 114 111 3 2 1 0 1 0 1 0 0 0 0 1 0 0 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagcaagtatgcc atgagctgggtccgccaggctccaggggaggggctgcagtgggtctcagca attagtggaaatggtgctgatata tactacgcagactccgtgaacggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgt gcgaattggctttcggggacacattctaagtttgactac ANWLSGTHSKFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcaccttcagcaagtatgcc atgagctgggtccgccaggctccaggggaggggctgcagtgggtctcagca attagtggaaatggtgctgatata tactacgcagactccgtgaacggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgt gcgaattggctttcggggacacattctaagtttgactac unmatched 16 213 10 6 6 10 4 6 3 2 3 8 JY8QFUQ01A3U1I 7049 productive Homsap IGHV3-11*01 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD4-17*01 F 2 8 8 15 8.8.15 [1.17.38.11] CARRRDYGDYGDGFDYW in-frame + ctctggattcatcttcagtgactactacatgacctggatccgccaggctccagggaaggggctggagtgggtttcatacattcgtagtaatgggagtcccatatacaacgcagactctgggaggggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaatagtctgagagtcgaggacacggccgtgtattactgtgcgagacggcgggactacggtgactacggtgacggctttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaagggttcttcccgctgagcctcgacagcacccccgaagatgggaacgtggtcgtcgcaatgcctggtccagggcttcttcccc NA unmatched, IGA2 36 63 [296, 293] c86>t,T29>I(- - -)|g119>c,S40>T(+ - +)|a169>c,S57>R(- - -)|g176>a,S59>N(- - -)|t186>g|a190>c,T64>P(+ + -)|t199>a,Y67>N(- - -)|t212>g,V71>G(- - -)|a215>g,K72>R(+ + +)|c276>t|c279>t|c287>t,A96>V(+ - +)| c86>t,T29>I(- - -)| g119>c,S40>T(+ - +)| a169>c,S57>R(- - -)|g176>a,S59>N(- - -)|t186>g|a190>c,T64>P(+ + -)| t199>a,Y67>N(- - -)|t212>g,V71>G(- - -)|a215>g,K72>R(+ + +)|c276>t|c279>t|c287>t,A96>V(+ - +)| g319>c|a320>g| 318 (320) 223 (225) 211 12 (14) 3 (5) 9 1 (2) 1 4 0 2 0 0 1 1 (2) 0 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 20 4 1 3 0 1 0 0 2 0 0 0 0 0 0 1 117 114 108 6 2 4 1 0 3 0 0 0 0 1 0 0 0 1 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagtgactactac atgacctggatccgccaggctccagggaaggggctggagtgggtttcatac attcgtagtaatgggagtcccata tacaacgcagactctgggaggggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaatagtctgagagtcgaggacacggccgtgtattactgt gcgagacggcgggactacggtgactacggtgacggctttgactac ARRRDYGDYGDGFDY IGHV3-11 IGHD4-17 IGHJ4 ggattcatcttcagtgactactac atgacctggatccgccaggctccagggaaggggctggagtgggtttcatac attcgtagtaatgggagtcccata tacaacgcagactctgggaggggccgattcaccatctccagggacaacgccaagaactcactgtatctgcaaatgaatagtctgagagtcgaggacacggccgtgtattactgt gcgagacggcgggactacggtgactacggtgacggctttgactac unmatched 12 213 6 6 5 6 1 6 2 1 5 4 JY8QFUQ01A4H9C 17896 productive Homsap IGHV3-48*02 F 896 90.37 197/218 nt NA Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 187 86 43/50 nt Homsap IGHD1-1*01 F 3 8 8 10 8.8.10 [1.17.38.11] CARDHIWAFDFW in-frame + cctctggattcaccttcagtgactatagcatgaactgggtccgccagactccagggaagggggtggagtggatttcatacatcggccgtggtggtgatgggatatactacgcagactctgtgaagggccgaatcaccatctccagagacaatgccaagaactcactttttctgcaaatgaacaccctgagagacgacgacacggctgtgtattactgtgcgagagaccacatttgggcttttgatttctggggccaagggacaaaggttaccgtctcgtcagcatcccccgaccagccccaaggtcttcccgctgagcctcgaagcaccccccaaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 64 79 [280] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g133>a,A45>T(- - -)|c148>g,L50>V(+ - +)|g157>a,V53>I(+ - +)|t168>c|a169>g,S57>G(+ + -)|t171>c,S57>G(+ + -)|a172>c,S58>R(- - -)|a175>g,S59>G(+ + -)|a184>g,S62>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a190>g,T64>G(+ - -)|c191>g,T64>G(+ - -)|c192>g,T64>G(+ - -)|t226>a,F76>I(+ - -)|g261>t|a263>t,Y88>F(- + -)|g278>c,S93>T(+ - +)|g291>c,E97>D(+ - +)| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g133>a,A45>T(- - -)|c148>g,L50>V(+ - +)|g157>a,V53>I(+ - +)| t168>c|a169>g,S57>G(+ + -)|t171>c,S57>G(+ + -)|a172>c,S58>R(- - -)|a175>g,S59>G(+ + -)|a184>g,S62>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a190>g,T64>G(+ - -)|c191>g,T64>G(+ - -)|c192>g,T64>G(+ - -)| t226>a,F76>I(+ - -)|g261>t|a263>t,Y88>F(- + -)|g278>c,S93>T(+ - +)|g291>c,E97>D(+ - +)| 320 226 205 21 2 19 6 4 0 2 1 0 1 1 2 3 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 48 3 0 3 0 2 0 0 0 0 0 0 0 1 0 0 30 24 13 11 1 10 5 1 0 2 1 0 0 0 0 2 0 0 117 114 109 5 1 4 0 0 0 0 0 0 1 1 2 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtgactatagc atgaactgggtccgccagactccagggaagggggtggagtggatttcatac atcggccgtggtggtgatgggata tactacgcagactctgtgaagggccgaatcaccatctccagagacaatgccaagaactcactttttctgcaaatgaacaccctgagagacgacgacacggctgtgtattactgt gcgagagaccacatttgggcttttgatttc ARDHIWAFDF IGHV3-48 IGHD1-1 IGHJ3 ggattcaccttcagtgactatagc atgaactgggtccgccagactccagggaagggggtggagtggatttcatac atcggccgtggtggtgatgggata tactacgcagactctgtgaagggccgaatcaccatctccagagacaatgccaagaactcactttttctgcaaatgaacaccctgagagacgacgacacggctgtgtattactgt gcgagagaccacatttgggcttttgatttc unmatched 21 213 12 9 4 10 8 11 1 1 7 12 JY8QFUQ01A55QU 6647 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 804 86.45 185/214 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-15*01 F 2 8 7 10 8.7.10 [1.17.38.11] CATLRGGTFDYW in-frame + ctctggtgcctccatcaggagttattattggagttggatccggcagcccccaggaaagggactggagtggattggttatattaattatgttggggacaccgattacaacccctccctcaagagtcgagtctccatgtcagcagccacgtccaagaaccaggtcttcctgcagctgacctctgtgaccgctgcggacaccgcctattatttctgtgcgacactgagagggggtactttcgactactggggccggggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG1 64 88 [276] g83>c,G28>A(- + -)|t105>g,S35>R(- - -)|c111>t|c114>t|c120>t|g141>a|g162>t|c168>t|t169>a,Y57>N(- - -)|c174>t|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a196>g,N66>D(+ + -)|c198>t,N66>D(+ + -)|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|t239>c,V80>A(+ - +)|a242>c,D81>A(- - -)|t259>g,F87>V(+ - -)|c263>t,S88>F(- - -)|a268>c,K90>Q(+ - -)|g275>c,S92>T(+ - +)|g297>c|g301>t,V101>Y(- - -)|t302>a,V101>Y(- - -)|g303>t,V101>Y(- - -)|a308>t,Y103>F(- + -)| g83>c,G28>A(- + -)|t105>g,S35>R(- - -)|c111>t|c114>t| c120>t|g141>a|g162>t| c168>t|t169>a,Y57>N(- - -)|c174>t|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a196>g,N66>D(+ + -)|c198>t,N66>D(+ + -)|a229>t,T77>S(+ - +)|a234>g,I78>M(+ + -)|t239>c,V80>A(+ - +)|a242>c,D81>A(- - -)|t259>g,F87>V(+ - -)|c263>t,S88>F(- - -)|a268>c,K90>Q(+ - -)|g275>c,S92>T(+ - +)|g297>c|g301>t,V101>Y(- - -)|t302>a,V101>Y(- - -)|g303>t,V101>Y(- - -)|a308>t,Y103>F(- + -)| g317>c,R106>T(- - -)|g319>c|a320>t| 316 (320) 218 (222) 189 (190) 29 (32) 8 (10) 21 (22) 4 2 7 1 2 0 2 (3) 2 3 (5) 0 4 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 0 2 0 0 0 0 0 1 0 0 1 51 51 48 3 3 0 0 1 1 0 0 0 0 0 0 0 1 0 30 21 14 7 2 5 2 1 2 0 0 0 0 1 0 0 1 0 117 114 99 15 1 14 2 0 2 1 2 0 2 1 2 0 2 1 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 0 0 0 0 0 0 (1) 0 0 (2) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtgcctccatcaggagttattat tggagttggatccggcagcccccaggaaagggactggagtggattggttat attaattatgttggggacacc gattacaacccctccctcaagagtcgagtctccatgtcagcagccacgtccaagaaccaggtcttcctgcagctgacctctgtgaccgctgcggacaccgcctattatttctgt gcgacactgagagggggtactttcgactac ATLRGGTFDY IGHV4-59 IGHD2-15 IGHJ4 ggtgcctccatcaggagttattat tggagttggatccggcagcccccaggaaagggactggagtggattggttat attaattatgttggggacacc gattacaacccctccctcaagagtcgagtctccatgtcagcagccacgtccaagaaccaggtcttcctgcagctgacctctgtgaccgctgcggacaccgcctattatttctgt gcgacactgagagggggtactttcgactac unmatched 29 210 14 15 9 16 5 13 4 4 14 7 JY8QFUQ01A56OJ 11387 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 981 94.93 206/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-8*01 F 2 8 8 8 8.8.8 [1.17.38.11] CARDNYGDYW in-frame + ctctggattcactttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtgttaatggtgatgggggtaggacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcacagtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [272, 273] c87>t|a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| c87>t| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|c192>g,S64>R(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| 320 225 214 11 5 6 4 1 1 0 0 0 0 0 3 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 3 0 0 0 0 0 0 0 0 1 0 0 117 114 108 6 4 2 1 1 0 0 0 0 0 0 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcactttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcactttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac unmatched 11 213 6 5 2 7 4 4 4 1 2 4 JY8QFUQ01A5K3B 9687 productive (see comment) Homsap IGHV1-3*01 F 930 92.99 199/214 nt 92.52 198/214 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 2 8 8 14 8.8.14 [0.17.38.11] CARGRYDISGRGLDYW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 37 of V-REGION: 'T' (1 nucleotides at position 21 to 21 in the user submitted sequence), (cause frameshift); tggatacaccttcactagttTttagtatacattgggtgcgccaggcccccggacagaggcttgagtggatgggatggatcaacgcaggcaacggtcacacaaaatattcacagaagttccaggacagagtcaccattataagggacacatccgcgagcacagcctacatggacctgagcaccctgagatctgaagacacggctgtctattactgtgcgagagggcgctatgatattagtggtcgtggccttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagcccccaaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA unmatched, IGA2 57 79 [290, 291, 288] c108>t|a110>t,Y37>F(- + -)|g112>a,A38>S(- + -)|c113>g,A38>S(- + -)|g117>a,M39>I(+ + -)|a144>g|t174>a|t186>c|a190>c,N64>H(- - -)|g221>a,G74>D(- - -)|c236>t,T79>I(- - -)|c237>a,T79>I(- - -)|g270>c,E90>D(+ - +)|g278>c,S93>T(+ - +)|g303>c| c108>t|a110>t,Y37>F(- + -)|g112>a,A38>S(- + -)|c113>g,A38>S(- + -)| g117>a,M39>I(+ + -)|a144>g| t174>a|t186>c|a190>c,N64>H(- - -)| g221>a,G74>D(- - -)|c236>t,T79>I(- - -)|c237>a,T79>I(- - -)|g270>c,E90>D(+ - +)|g278>c,S93>T(+ - +)|g303>c| a320>g| 319 (320) 221 (222) 206 15 (16) 5 (6) 10 1 (2) 3 2 1 1 1 1 1 3 1 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 1 0 0 0 1 0 0 1 0 0 51 51 49 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 2 1 0 0 0 1 1 0 0 1 0 0 0 0 117 114 108 6 1 5 0 1 1 0 0 1 0 0 3 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|ta,262-263(FR3)|aa,290-291(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,11-12(FR1)|tt,62-63(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,120-121(FR2)|tt,149-150(FR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,201-202(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcactagttttagt atacattgggtgcgccaggcccccggacagaggcttgagtggatgggatgg atcaacgcaggcaacggtcacaca aaatattcacagaagttccaggacagagtcaccattataagggacacatccgcgagcacagcctacatggacctgagcaccctgagatctgaagacacggctgtctattactgt gcgagagggcgctatgatattagtggtcgtggccttgactac ARGRYDISGRGLDY IGHV1-3 IGHD3-22 IGHJ4 ggatacaccttcactagttttagt atacattgggtgcgccaggcccccggacagaggcttgagtggatgggatgg atcaacgcaggcaacggtcacaca aaatattcacagaagttccaggacagagtcaccattataagggacacatccgcgagcacagcctacatggacctgagcaccctgagatctgaagacacggctgtctattactgt gcgagagggcgctatgatattagtggtcgtggccttgactac unmatched 15 213 7 8 5 10 2 5 2 3 6 4 JY8QFUQ01A6DSA 6052 productive (see comment) Homsap IGHV3-48*01 F (see comment) 759 84.11 180/214 nt 83.18 178/214 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-12*01 F 3 8 7 12 8.7.12 [1.17.38.11] CSRDGRRGYDLDSW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (84,11% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 57 to 57 in the user submitted sequence), (cause frameshift); in CDR2-IMGT, from codon 57 of V-REGION: 3 nucleotides (from position 83 in the user submitted sequence), (do not cause frameshift) ctctggattcaccttcggaacctatgccatgacgtgggtccgcctgactcctgggaAaagggctggagtgggtttcatggattagtgatatcggtgacacacgctatgcagattctgtgaagggccgattcaccatctccagagacaatgccaagaattcactgtttctgcaaatggacagtctcagagccgacgacacggctatatattattgttcgagagatggccggaggggctacgaccttgactcctggggccagggagccctggtcaccgtctcctcatcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [282, 283] a103>g,S35>G(+ + -)|t105>a,S35>G(+ + -)|g107>c,S36>T(+ - +)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)|a119>c,N40>T(- + -)|c120>g,N40>T(- + -)|a131>t,Q44>L(- - -)|g133>a,A45>T(- - -)|a138>t|g144>a|a164>g,Y55>W(- + -)|c165>g,Y55>W(- + -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g185>t,S62>I(- - -)|t186>c,S62>I(- - -)|a187>g,S63>G(+ + -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|t194>c,I65>T(- - -)|t196>c,Y66>R(- - -)|a197>g,Y66>R(- - -)|c201>t|c207>t|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|g282>c|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t|g313>t,A105>S(- + -)| a103>g,S35>G(+ + -)|t105>a,S35>G(+ + -)|g107>c,S36>T(+ - +)|a112>g,S38>A(- + -)|g113>c,S38>A(- + -)| a119>c,N40>T(- + -)|c120>g,N40>T(- + -)|a131>t,Q44>L(- - -)|g133>a,A45>T(- - -)|a138>t|g144>a|a164>g,Y55>W(- + -)|c165>g,Y55>W(- + -)| a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g185>t,S62>I(- - -)|t186>c,S62>I(- - -)|a187>g,S63>G(+ + -)|a190>g,T64>D(- + -)|c191>a,T64>D(- + -)|t194>c,I65>T(- - -)| t196>c,Y66>R(- - -)|a197>g,Y66>R(- - -)|c201>t|c207>t|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|g282>c|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| g313>t,A105>S(- + -)| 320 222 187 35 8 27 8 5 5 3 1 1 3 1 4 2 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 2 0 0 0 0 0 0 1 2 0 0 0 51 51 43 8 2 6 1 2 0 0 1 0 2 0 0 2 0 0 30 21 13 8 0 8 3 1 0 2 0 1 0 0 0 0 1 0 117 114 101 13 6 7 2 2 5 1 0 0 1 0 2 0 0 0 8 8 7 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcggaacctatgcc atgacgtgggtccgcctgactcctgggaaagggctggagtgggtttcatgg attagtgatatcggtgacaca cgctatgcagattctgtgaagggccgattcaccatctccagagacaatgccaagaattcactgtttctgcaaatggacagtctcagagccgacgacacggctatatattattgt tcgagagatggccggaggggctacgaccttgactcc SRDGRRGYDLDS IGHV3-48 IGHD5-12 IGHJ4 ggattcaccttcggaacctatgcc atgacgtgggtccgcctgactcctgggaaagggctggagtgggtttcatgg attagtgatatcggtgacaca cgctatgcagattctgtgaagggccgattcaccatctccagagacaatgccaagaattcactgtttctgcaaatggacagtctcagagccgacgacacggctatatattattgt tcgagagatggccggaggggctacgaccttgactcc unmatched 34 210 21 13 10 18 11 16 8 0 13 13 JY8QFUQ01A6FSC 18432 productive Homsap IGHV3-7*01 F 909 91.24 198/217 nt NA Homsap IGHJ5*02 F 102 66.67 34/51 nt Homsap IGHD2-2*01 F 3 8 8 10 8.8.10 [1.17.38.11] CARDSPSAYDPW in-frame + ctctggattcacctttagttatcactggatgagttgggtccgccaggctccagggaaggggctggagtgggtggccctcataaggcaagatggaagtgaggaatactatgtggactctgtgaggggccgattcagcatctccagagacaacgccaagaattcagtgtacttggaaatgaacaacctgagagccgaggacacggctgtttattactgtgcgagagattccccctcagcctacgatccctggggccagggaaccctggtcaccgctctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgacgagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG2 64 88 [280] a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c108>t,S36>Y(+ - -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)|c120>t|a163>c,N55>L(- - -)|a164>t,N55>L(- - -)|a170>g,K57>R(+ + +)|a193>g,K65>E(+ - -)|a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|c255>t|c259>g,L87>V(+ - +)|t264>c|c265>t|c268>g,Q90>E(+ + -)|g278>a,S93>N(- - -)|g303>t| a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c108>t,S36>Y(+ - -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)| c120>t|a163>c,N55>L(- - -)|a164>t,N55>L(- - -)| a170>g,K57>R(+ + +)|a193>g,K65>E(+ - -)| a215>g,K72>R(+ + +)|c230>g,T77>S(+ - +)|c255>t|c259>g,L87>V(+ - +)|t264>c|c265>t|c268>g,Q90>E(+ + -)|g278>a,S93>N(- - -)|g303>t| 320 225 206 19 5 14 3 2 4 3 1 0 2 0 0 3 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 1 1 2 0 0 1 0 0 0 0 0 51 51 48 3 1 2 0 0 1 0 1 0 1 0 0 0 0 0 30 24 22 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 117 114 105 9 4 5 1 1 2 1 0 0 0 0 0 3 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagttatcactgg atgagttgggtccgccaggctccagggaaggggctggagtgggtggccctc ataaggcaagatggaagtgaggaa tactatgtggactctgtgaggggccgattcagcatctccagagacaacgccaagaattcagtgtacttggaaatgaacaacctgagagccgaggacacggctgtttattactgt gcgagagattccccctcagcctacgatccc ARDSPSAYDP IGHV3-7 IGHD2-2 IGHJ5 ggattcacctttagttatcactgg atgagttgggtccgccaggctccagggaaggggctggagtgggtggccctc ataaggcaagatggaagtgaggaa tactatgtggactctgtgaggggccgattcagcatctccagagacaacgccaagaattcagtgtacttggaaatgaacaacctgagagccgaggacacggctgtttattactgt gcgagagattccccctcagcctacgatccc unmatched 19 213 12 7 6 10 6 9 5 0 7 7 JY8QFUQ01A6GAE 1496 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 810 86.18 187/217 nt 85.71 186/217 nt Homsap IGHJ2*01 F (see comment) 130 71.7 38/53 nt Homsap IGHD3-10*01 F 2 8 8 14 8.8.14 [1.17.38.11] CVKEDHDSGLRVLLEW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 and Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 51 of V-REGION: 'AG' (2 nucleotides at position 67 to 68 in the user submitted sequence), (cause frameshift); ctctggattcacgtttggcatctatgccatgagttgggtccgccaggctccagggaggggcgtggaAGgtgggtcgcaagcatgggtaatagtgctggcagtacatactacgcaggctccgtgaagggtcgcttcaacatctccagagacaattccaagaaaaccctgtatcttcaaatggacagcctgagagtcgacgacacggccagatattactgtgtgaaagaagaccatgattcgggcctcagggtcctccttgagtggggccggggaaccctggtgactgtctccgcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccctaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [292, 293] c87>g|a103>g,S35>G(+ + -)|g107>t,S36>I(- - -)|c120>t|a143>g,K48>R(+ + +)|g147>c|c148>g,L50>V(+ - +)|t160>g,S54>A(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t165>c,A55>S(- + -)|t168>g,I56>M(+ + -)|a169>g,S57>G(+ + -)|g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|g185>c,G62>A(- + -)|t189>c|c192>t|a206>g,D69>G(- - -)|c222>t|g225>c|c230>a,T77>N(- + -)|c255>a,N85>K(+ - -)|g258>c|g267>t|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)|c314>t,A105>V(+ - +)| c87>g|a103>g,S35>G(+ + -)|g107>t,S36>I(- - -)| c120>t|a143>g,K48>R(+ + +)|g147>c|c148>g,L50>V(+ - +)|t160>g,S54>A(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t165>c,A55>S(- + -)| t168>g,I56>M(+ + -)|a169>g,S57>G(+ + -)|g172>a,G58>N(- - -)|g173>a,G58>N(- - -)|g185>c,G62>A(- + -)|t189>c|c192>t| a206>g,D69>G(- - -)|c222>t|g225>c|c230>a,T77>N(- + -)|c255>a,N85>K(+ - -)|g258>c|g267>t|a274>g,N92>D(+ + -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)| c314>t,A105>V(+ - +)| 320 225 194 31 9 22 5 4 5 2 0 2 0 0 5 3 2 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 1 0 0 0 0 0 0 0 0 1 1 0 51 51 43 8 2 6 1 1 1 1 0 0 0 0 1 2 0 1 30 24 17 7 2 5 1 2 1 1 0 0 0 0 1 0 0 1 117 114 102 12 4 8 2 1 2 0 0 2 0 0 3 0 1 1 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgtttggcatctatgcc atgagttgggtccgccaggctccagggaggggcgtggagtgggtcgcaagc atgggtaatagtgctggcagtaca tactacgcaggctccgtgaagggtcgcttcaacatctccagagacaattccaagaaaaccctgtatcttcaaatggacagcctgagagtcgacgacacggccagatattactgt gtgaaagaagaccatgattcgggcctcagggtcctccttgag VKEDHDSGLRVLLE IGHV3-23 IGHD3-10 IGHJ2 ggattcacgtttggcatctatgcc atgagttgggtccgccaggctccagggaggggcgtggagtgggtcgcaagc atgggtaatagtgctggcagtaca tactacgcaggctccgtgaagggtcgcttcaacatctccagagacaattccaagaaaaccctgtatcttcaaatggacagcctgagagtcgacgacacggccagatattactgt gtgaaagaagaccatgattcgggcctcagggtcctccttgag unmatched 30 213 15 15 8 20 7 10 6 3 14 7 JY8QFUQ01A72DW 16654 productive Homsap IGHV3-7*01 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD3-10*01 F 2 8 8 18 8.8.18 [1.17.38.11] CAKARIYFYGSGSYSLFDYW in-frame + ctctggaatcaccttgagtccctattggatgacctgggtccgccaggctcccgggaaggggctggagtgggtggccaacataaaccaagatggaggtgagagaaattatgtggcctctgtgaggggccggttcaccatctccagagacaacgccaggaattcactgtatctgcaaatgaacagcctgagagtcgacgacacggctgtatattattgtgcaaaagccaggatctatttttatggttcggggagttattccctctttgactactggggccagggagccctggtcaccgtctcctcagcctccaccaaggggcccatcggtcttccccctggccaccctcctccaagagcacctctggggccacagcggccctgggctgcctggtcaaggactacttcccgaaccggtgacgtgtcgtggaactca NA unmatched, IGG1 57 75 [305, 304] t82>a,F28>I(+ - -)|t90>g,F30>L(+ - -)|a106>c,S36>P(+ - -)|g107>c,S36>P(+ - -)|g119>c,S40>T(+ - +)|a138>c|g171>c,K57>N(+ - -)|a187>g,S63>G(+ + -)|a194>g,K65>R(+ + +)|t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a225>g|a251>g,K84>R(+ + +)|c255>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>a|c309>t|g315>a|g317>a,R106>K(+ + +)| t82>a,F28>I(+ - -)|t90>g,F30>L(+ - -)|a106>c,S36>P(+ - -)|g107>c,S36>P(+ - -)| g119>c,S40>T(+ - +)|a138>c| g171>c,K57>N(+ - -)|a187>g,S63>G(+ + -)|a194>g,K65>R(+ + +)| t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a225>g|a251>g,K84>R(+ + +)|c255>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>a|c309>t| g315>a|g317>a,R106>K(+ + +)|a320>c| 319 (320) 224 (225) 202 22 (23) 6 (7) 16 5 3 4 0 3 (4) 0 0 2 4 0 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 0 1 0 0 1 1 0 0 1 51 51 49 2 1 1 0 0 0 0 1 0 0 0 1 0 0 0 30 24 21 3 0 3 2 0 0 0 0 0 0 0 1 0 0 0 117 114 103 11 4 7 3 1 4 0 1 0 0 1 1 0 0 0 7 (8) 7 5 2 (3) 1 (2) 1 0 2 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggaatcaccttgagtccctattgg atgacctgggtccgccaggctcccgggaaggggctggagtgggtggccaac ataaaccaagatggaggtgagaga aattatgtggcctctgtgaggggccggttcaccatctccagagacaacgccaggaattcactgtatctgcaaatgaacagcctgagagtcgacgacacggctgtatattattgt gcaaaagccaggatctatttttatggttcggggagttattccctctttgactac AKARIYFYGSGSYSLFDY IGHV3-7 IGHD3-10 IGHJ4 ggaatcaccttgagtccctattgg atgacctgggtccgccaggctcccgggaaggggctggagtgggtggccaac ataaaccaagatggaggtgagaga aattatgtggcctctgtgaggggccggttcaccatctccagagacaacgccaggaattcactgtatctgcaaatgaacagcctgagagtcgacgacacggctgtatattattgt gcaaaagccaggatctatttttatggttcggggagttattccctctttgactac unmatched 20 213 10 10 5 9 5 11 5 0 8 7 JY8QFUQ01A75AZ 9357 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 999 95.85 208/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 8 8 8.8.8 [1.17.38.11] CARDNYGDYW in-frame + ctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtgttaatggtgatgggggtagcacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [272, 273] a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| 320 225 216 9 4 5 4 1 0 0 0 0 0 0 3 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 3 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 4 2 1 1 0 0 0 0 0 0 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac unmatched 9 213 5 4 1 5 4 4 4 0 2 3 JY8QFUQ01A7UAO 20362 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 855 88.48 192/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-15*01 F 3 8 8 14 8.8.14 [1.17.38.11] CAKEEGATGVYYVDHW in-frame + ctctggattcacctttaccacctccgccatggcctgggtccgccaggttccagggaaggggctggagtgggtctcaactattagacctagtggtgagagaacctactacgcagagtccgtgaggggccgcttcaccatctccagagacaattccgagaacacgttgtatctacaactgaacaacctgagagtcgaggacacggccatatattactgtgcgaaggaggaaggtgctactggagtctattatgttgaccactggggccagggaacccgggtcaccgtctcctcatcctccaccgaagggcccagtcggtccttcccctggcgccctgctccaggagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG2 93 62 [293] g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|g163>a,A55>T(- - -)|t171>a,S57>R(- - -)|g172>c,G58>P(+ - -)|g173>c,G58>P(+ - -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)|a195>c|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|g225>c|a250>g,K84>E(+ - -)|c259>t|g267>a|a271>c,M91>L(+ + -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)|a318>g| g104>c,S35>T(+ - +)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|t111>c,Y37>S(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|g163>a,A55>T(- - -)| t171>a,S57>R(- - -)|g172>c,G58>P(+ - -)|g173>c,G58>P(+ - -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)|a195>c| c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|g225>c|a250>g,K84>E(+ - -)|c259>t|g267>a|a271>c,M91>L(+ + -)|g278>a,S93>N(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>I(+ - +)| a318>g| 320 225 199 26 5 21 4 5 3 1 3 1 0 1 6 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 1 1 0 0 0 2 0 0 0 51 51 47 4 0 4 1 1 1 0 0 0 0 0 1 0 0 0 30 24 17 7 1 6 0 1 0 0 1 1 0 1 2 0 0 1 117 114 104 10 3 7 2 3 2 0 1 0 0 0 1 1 0 0 8 8 7 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaccacctccgcc atggcctgggtccgccaggttccagggaaggggctggagtgggtctcaact attagacctagtggtgagagaacc tactacgcagagtccgtgaggggccgcttcaccatctccagagacaattccgagaacacgttgtatctacaactgaacaacctgagagtcgaggacacggccatatattactgt gcgaaggaggaaggtgctactggagtctattatgttgaccac AKEEGATGVYYVDH IGHV3-23 IGHD2-15 IGHJ4 ggattcacctttaccacctccgcc atggcctgggtccgccaggttccagggaaggggctggagtgggtctcaact attagacctagtggtgagagaacc tactacgcagagtccgtgaggggccgcttcaccatctccagagacaattccgagaacacgttgtatctacaactgaacaacctgagagtcgaggacacggccatatattactgt gcgaaggaggaaggtgctactggagtctattatgttgaccac unmatched 25 213 12 13 8 16 4 9 3 1 11 10 JY8QFUQ01A9CSP 5522 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F (see comment) 765 83.87 182/217 nt NA Homsap IGHJ6*02 F 265 91.94 57/62 nt Homsap IGHD6-25*01 F 1 8 8 22 8.8.22 [1.17.38.11] CARYQGDTSGYQDVFYAHYGMDVW in-frame + Low V-REGION identity (83,87% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctggattcacttttaggggctactggatgcactgggtccgtcaggttccaggtaaggcgccggagtggctcgcacgtctgaatactgatggagatagtacaagttatgcggactccgtgaagggccgcttcaccatctccagagacaacgccaggagcacattgttcctgcaaatgagcagtctgagagtcgaagacacggccatttattactgtgcacggtatcagggcgataccagtggctaccaggacgttttctacgcccactacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgccctggtccagggcttcttccc NA unmatched, IGA2 50 84 [314, 315] c87>t|c90>t|t105>g,S35>R(- - -)|a106>g,S36>G(+ + -)|c129>t|a132>g|c134>t,A45>V(+ - +)|g141>t|g146>c,G49>A(- + -)|t149>c,L50>P(- - -)|t152>a,V51>E(- + -)|g157>c,V53>L(+ - +)|t160>g,S54>A(- + -)|a166>c,I56>L(+ + +)|t168>g,I56>L(+ + +)|g173>c,S58>T(+ - +)|g186>a|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|c192>t|c198>t|c201>t|a225>c|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|g258>a|c259>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|g291>a|t300>c|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| c87>t|c90>t|t105>g,S35>R(- - -)|a106>g,S36>G(+ + -)| c129>t|a132>g|c134>t,A45>V(+ - +)|g141>t|g146>c,G49>A(- + -)|t149>c,L50>P(- - -)|t152>a,V51>E(- + -)|g157>c,V53>L(+ - +)|t160>g,S54>A(- + -)| a166>c,I56>L(+ + +)|t168>g,I56>L(+ + +)|g173>c,S58>T(+ - +)|g186>a|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|c192>t| c198>t|c201>t|a225>c|a251>g,K84>R(+ + +)|a254>g,N85>S(- - -)|g258>a|c259>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|g291>a|t300>c|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| a316>c|a318>g|g319>t| 315 (320) 220 (225) 185 (187) 35 (38) 14 (17) 21 6 (7) 5 9 3 2 (3) 0 1 1 3 0 2 (3) 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 1 0 2 0 0 0 0 0 0 0 0 1 51 51 42 9 3 6 1 0 2 1 0 0 0 1 2 0 1 1 30 24 17 7 2 5 1 2 1 0 1 0 0 0 1 0 0 1 117 114 99 15 7 8 3 3 4 2 1 0 1 0 0 0 1 0 3 (8) 3 3 (5) 0 (3) 0 (3) 0 0 (1) 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacttttaggggctactgg atgcactgggtccgtcaggttccaggtaaggcgccggagtggctcgcacgt ctgaatactgatggagatagtaca agttatgcggactccgtgaagggccgcttcaccatctccagagacaacgccaggagcacattgttcctgcaaatgagcagtctgagagtcgaagacacggccatttattactgt gcacggtatcagggcgataccagtggctaccaggacgttttctacgcccactacggtatggacgtc ARYQGDTSGYQDVFYAHYGMDV IGHV3-74 IGHD6-25 IGHJ6 ggattcacttttaggggctactgg atgcactgggtccgtcaggttccaggtaaggcgccggagtggctcgcacgt ctgaatactgatggagatagtaca agttatgcggactccgtgaagggccgcttcaccatctccagagacaacgccaggagcacattgttcctgcaaatgagcagtctgagagtcgaagacacggccatttattactgt gcacggtatcagggcgataccagtggctaccaggacgttttctacgcccactacggtatggacgtc unmatched 35 213 23 12 14 19 9 16 10 4 14 7 JY8QFUQ01A9KBS 5733 productive (see comment) Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 929 91.4 202/221 nt 90.5 200/221 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 2 10 7 14 10.7.14 [1.17.38.11] CARRALDSSGHYIDIW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 56 to 56 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 70 of V-REGION: 'C' (1 nucleotides at position 122 to 122 in the user submitted sequence), (cause frameshift); tctctggtggctccatgagcagtggtaattactgctggggctggggccgccagccCcccaggaaaggggctggagtggattggaagtatgtgttatggtgggagcacctactacagcttgtCcccccaagggtcgagtcaccatatccatagactcgtcgaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggctgtgtattactgtgcgagacgcgcattagacagtagtggtcattacattgacatctggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggttcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA unmatched, IGA2 57 79 [298, 297, 296] c90>g,I30>M(+ + -)|a103>g,S35>G(+ + -)|g107>a,S36>N(- - -)|a113>g,Y38>C(- - -)|a124>g,I42>G(- - -)|t125>g,I42>G(- - -)|g141>a|g162>a|c168>g,I56>M(+ + -)|a170>g,Y57>C(- - -)|a175>g,S59>G(+ + -)|a203>g,N68>S(- - -)|c205>t,P69>L(- - -)|c206>t,P69>L(- - -)|t212>c,L71>P(- - -)|a220>g,S74>G(+ + -)|g238>a,V80>I(+ - +)|a244>t,T82>S(+ - +)|c249>g| c90>g,I30>M(+ + -)|a103>g,S35>G(+ + -)|g107>a,S36>N(- - -)|a113>g,Y38>C(- - -)| a124>g,I42>G(- - -)|t125>g,I42>G(- - -)|g141>a|g162>a| c168>g,I56>M(+ + -)|a170>g,Y57>C(- - -)|a175>g,S59>G(+ + -)| a203>g,N68>S(- - -)|c205>t,P69>L(- - -)|c206>t,P69>L(- - -)|t212>c,L71>P(- - -)|a220>g,S74>G(+ + -)|g238>a,V80>I(+ - +)|a244>t,T82>S(+ - +)|c249>g| a320>g| 319 (320) 228 (229) 209 19 (20) 3 (4) 16 7 (8) 4 2 1 0 0 1 0 0 3 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 0 4 2 1 0 0 0 0 0 0 0 1 0 0 51 51 47 4 2 2 1 2 0 0 0 0 0 0 0 0 0 1 30 21 18 3 0 3 2 0 0 0 0 0 0 0 0 1 0 0 117 114 106 8 1 7 2 1 2 1 0 0 1 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatgagcagtggtaattactgc tggggctggggccgccagcccccaggaaaggggctggagtggattggaagt atgtgttatggtgggagcacc tactacagcttgtcccccaagggtcgagtcaccatatccatagactcgtcgaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggctgtgtattactgt gcgagacgcgcattagacagtagtggtcattacattgacatc ARRALDSSGHYIDI IGHV4-39 IGHD3-22 IGHJ4 ggtggctccatgagcagtggtaattactgc tggggctggggccgccagcccccaggaaaggggctggagtggattggaagt atgtgttatggtgggagcacc tactacagcttgtcccccaagggtcgagtcaccatatccatagactcgtcgaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggctgtgtattactgt gcgagacgcgcattagacagtagtggtcattacattgacatc unmatched 19 216 14 5 6 9 8 10 3 0 9 7 JY8QFUQ01A9XMX 12391 productive Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 1065 97.76 218/223 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD4-17*01 F 3 8 10 15 8.10.15 [1.17.38.11] CTTFGLTTVTKGYFDFW in-frame + ctctggattcactttcaataacgcctggatgagctgggtccgccaggctccagggaaggggctggagtgggttggccgtattaaaagcaaaactgatggtgggacaacagagtacgctgcacccgtgaaaggcagatttaccatctcaagagatgattcaaaaaacacgctttatctgcaaatgaacagcctgaaaatcgaggacacagccgtgtattactgtaccacattcggcctgactacggtgactaaaggctactttgacttctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcaccccagcccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA unmatched, IGA1 57 89 [299, 301] g104>a,S35>N(- - -)|c198>g,D66>E(+ - +)|c228>t|g261>t|c287>t,T96>I(- - -)| g104>a,S35>N(- - -)| c198>g,D66>E(+ - +)|c228>t|g261>t|c287>t,T96>I(- - -)| g319>t|a320>t| 318 (320) 229 (231) 224 5 (7) 2 (4) 3 0 1 2 0 0 0 0 (1) 0 0 1 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 110 4 2 2 0 0 2 0 0 0 0 0 0 1 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| ctct ggattcactttcaataacgcctgg atgagctgggtccgccaggctccagggaaggggctggagtgggttggccgt attaaaagcaaaactgatggtgggacaaca gagtacgctgcacccgtgaaaggcagatttaccatctcaagagatgattcaaaaaacacgctttatctgcaaatgaacagcctgaaaatcgaggacacagccgtgtattactgt accacattcggcctgactacggtgactaaaggctactttgacttc TTFGLTTVTKGYFDF IGHV3-15 IGHD4-17 IGHJ4 ggattcactttcaataacgcctgg atgagctgggtccgccaggctccagggaaggggctggagtgggttggccgt attaaaagcaaaactgatggtgggacaaca gagtacgctgcacccgtgaaaggcagatttaccatctcaagagatgattcaaaaaacacgctttatctgcaaatgaacagcctgaaaatcgaggacacagccgtgtattactgt accacattcggcctgactacggtgactaaaggctactttgacttc unmatched 5 219 3 2 3 5 0 0 2 0 2 1 JY8QFUQ01AAWJ6 20374 productive Homsap IGHV3-48*01 F 995 95.41 208/218 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 3 8 8 13 8.8.13 [1.17.38.11] CARERGNSYGEIDYW in-frame + cctctggtttcaccttcaggagctctggcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagcagtagtactactaccaaatactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagaacgtggaaacagctatggagagattgactactggggccagggaaccctggtcaccgtctcctcagcttccaccaagggcccaggagcacctccgagagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG4 64 50 [261, 289] a81>t|t105>g,S35>R(- - -)|a110>c,Y37>S(+ - -)|a112>g,S38>G(+ + -)|t171>c|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t194>a,I65>K(- + -)|a200>g,Y67>C(- - -)|a254>g,N85>S(- - -)| a81>t|t105>g,S35>R(- - -)|a110>c,Y37>S(+ - -)|a112>g,S38>G(+ + -)| t171>c|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|t194>a,I65>K(- + -)| a200>g,Y67>C(- - -)|a254>g,N85>S(- - -)| 320 226 216 10 2 8 3 0 0 1 1 0 1 1 2 0 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 0 0 1 0 1 0 0 0 0 1 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 0 0 0 1 0 0 0 1 2 0 0 0 117 114 112 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggtttcaccttcaggagctctggc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagcagtagtactactaccaaa tactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagaacgtggaaacagctatggagagattgactac ARERGNSYGEIDY IGHV3-48 IGHD5-18 IGHJ4 ggtttcaccttcaggagctctggc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagcagtagtactactaccaaa tactgcgcagactctgtgaagggccgattcaccatctccagagacaatgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagaacgtggaaacagctatggagagattgactac unmatched 10 213 4 6 0 2 4 8 0 2 2 6 JY8QFUQ01AB8R5 10698 productive Homsap IGHV3-64*02 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 2 8 8 10 8.8.10 [1.17.38.11] CVTCGDYSYEYW in-frame + ctctggattcattttcagtacttatcctatgcactgggtccgccaggctccagggaagggactggaatatgtttcagctattagtcgtaatggggataacgcatattatgcagactctgtgaagggcagattcaccatgtccagagacaattccaagagcacactgtatcttcagatgggcagcctgagagctgaggacatggctgtgtattactgtgtgacatgtggtgattacagctatgagtactggggccagggaaccttggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [278, 279] c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>c,A38>P(- - -)|a172>c,S58>R(- - -)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|a193>g,T65>A(- - -)|c234>g,I78>M(+ + -)|a254>g,N85>S(- - -)|g258>a|a270>g|c314>t,A105>V(+ - +)| c86>t,T29>I(- - -)|c87>t,T29>I(- - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>c,A38>P(- - -)| a172>c,S58>R(- - -)|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|a193>g,T65>A(- - -)| c234>g,I78>M(+ + -)|a254>g,N85>S(- - -)|g258>a|a270>g| c314>t,A105>V(+ - +)|g317>c,R106>T(- - -)|g319>t|a320>g| 316 (320) 221 (225) 207 (208) 14 (17) 2 (4) 12 (13) 3 (4) 3 4 0 1 0 0 0 2 (3) 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 0 3 0 0 0 0 0 2 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 2 0 0 1 0 0 0 0 0 0 0 117 114 110 4 2 2 2 1 0 0 0 0 0 0 0 1 0 0 4 (8) 4 3 (4) 1 (4) 0 (2) 1 (2) 0 (1) 0 1 0 0 0 0 0 0 (1) 0 0 (1) 0 aa,26-27(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|aa,152-153(FR2)|ta,154-155(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,154-155(FR2)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|tt,198-199(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|agct,162-165(FR2)|agta,169-172(CDR2)|ggta,187-190(CDR2)|ggca,220-223(FR3)|ggca,274-277(FR3)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|agct,162-165(FR2)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcattttcagtacttatcct atgcactgggtccgccaggctccagggaagggactggaatatgtttcagct attagtcgtaatggggataacgca tattatgcagactctgtgaagggcagattcaccatgtccagagacaattccaagagcacactgtatcttcagatgggcagcctgagagctgaggacatggctgtgtattactgt gtgacatgtggtgattacagctatgagtac VTCGDYSYEY IGHV3-64 IGHD3-22 IGHJ4 ggattcattttcagtacttatcct atgcactgggtccgccaggctccagggaagggactggaatatgtttcagct attagtcgtaatggggataacgca tattatgcagactctgtgaagggcagattcaccatgtccagagacaattccaagagcacactgtatcttcagatgggcagcctgagagctgaggacatggctgtgtattactgt gtgacatgtggtgattacagctatgagtac unmatched 13 213 9 4 6 9 3 4 2 0 2 9 JY8QFUQ01ABJSU 10834 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 936 92.63 201/217 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [1.17.38.11] CTRDIGGRYSYW in-frame + ctccggattcacctttagtagttactggatgcactgggtccgccaaactccaggggagggactggtgtgggtctcacggactaatgaagatgggagtatcacaaactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgtacaagagacatcggtgggcggtactcctactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [278, 279] t78>c|c90>t|c108>t|g133>a,A45>T(- - -)|a142>g,K48>E(+ - -)|g147>a|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|g313>a,A105>T(- - -)| t78>c| c90>t|c108>t| g133>a,A45>T(- - -)|a142>g,K48>E(+ - -)|g147>a|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)| g313>a,A105>T(- - -)| 320 225 208 17 5 12 3 6 2 2 0 0 0 1 0 1 1 1 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 22 2 2 0 0 0 2 0 0 0 0 0 0 0 0 0 51 51 47 4 2 2 1 2 0 0 0 0 0 0 0 0 0 1 30 24 19 5 0 5 1 1 0 1 0 0 0 1 0 0 1 0 117 114 110 4 0 4 1 2 0 0 0 0 0 0 0 1 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcacctttagtagttactgg atgcactgggtccgccaaactccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt acaagagacatcggtgggcggtactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttagtagttactgg atgcactgggtccgccaaactccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt acaagagacatcggtgggcggtactcctac unmatched 15 213 11 4 7 9 4 6 2 2 6 5 JY8QFUQ01ACF7S 894 productive Homsap IGHV3-7*01 F 949 93.52 202/216 nt NA Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD3-10*01 F 3 8 8 13 8.8.13 [1.17.38.11] CARAIGRPFYGMDVW in-frame + tctggattctcctttgatatatattggatgagatgggtccgcctggctccagggaaggggctggagtgtgtggccgacataaagcaagatggaagtgagaagtactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtttctgcaaatgaacagcctgagagtcgaggacacgggtgtgtatttctgtgcgagagcgatcggacggcccttctacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagcatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 84 [286] a85>t,T29>S(+ - +)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>t,S36>I(- - -)|c108>a,S36>I(- - -)|c120>a,S40>R(- - -)|a131>t,Q44>L(- - -)|g156>t,W52>C(+ - -)|a163>g,N55>D(+ + -)|a195>g|a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|c299>g,A100>G(- + -)|a308>t,Y103>F(- + -)| a85>t,T29>S(+ - +)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>t,S36>I(- - -)|c108>a,S36>I(- - -)| c120>a,S40>R(- - -)|a131>t,Q44>L(- - -)|g156>t,W52>C(+ - -)|a163>g,N55>D(+ + -)| a195>g| a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|c299>g,A100>G(- + -)|a308>t,Y103>F(- + -)| a320>c| 319 (320) 223 (224) 209 14 (15) 1 (2) 13 3 1 1 0 0 (1) 2 4 0 0 1 2 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 1 0 0 0 1 1 0 0 0 1 0 51 51 47 4 0 4 1 0 0 0 0 1 1 0 0 0 1 0 30 24 23 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 117 114 110 4 0 4 0 0 1 0 0 0 2 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattctcctttgatatatattgg atgagatgggtccgcctggctccagggaaggggctggagtgtgtggccgac ataaagcaagatggaagtgagaag tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtttctgcaaatgaacagcctgagagtcgaggacacgggtgtgtatttctgt gcgagagcgatcggacggcccttctacggtatggacgtc ARAIGRPFYGMDV IGHV3-7 IGHD3-10 IGHJ6 ggattctcctttgatatatattgg atgagatgggtccgcctggctccagggaaggggctggagtgtgtggccgac ataaagcaagatggaagtgagaag tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtttctgcaaatgaacagcctgagagtcgaggacacgggtgtgtatttctgt gcgagagcgatcggacggcccttctacggtatggacgtc unmatched 14 213 5 9 2 7 3 7 0 1 8 5 JY8QFUQ01ACGK8 7777 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 977 94.5 206/218 nt NA Homsap IGHJ5*02 F 219 92.16 47/51 nt Homsap IGHD6-13*01 F 2 8 8 16 8.8.16 [1.17.38.11] CARGRIVSVGMSGWFDPW in-frame + cctctggattcaccttcagtcagtactggatgtactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtattaatggtgatggaagtagcacaagctatgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtatattattgtgcaagaggccgtatagtatcagttgggatgtcaggctggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatcccgaccagccccaaggtctcccgctgagctctgcagcaccccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccgag NA unmatched, IGA1 64 74 [295, 296] a106>c,S36>Q(- - -)|g107>a,S36>Q(- - -)|c108>g,S36>Q(- - -)|c118>t,H40>Y(+ - -)|a172>g,S58>G(+ + -)|g186>a|c201>t|g258>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>a|c309>t| a106>c,S36>Q(- - -)|g107>a,S36>Q(- - -)|c108>g,S36>Q(- - -)| c118>t,H40>Y(+ - -)| a172>g,S58>G(+ + -)|g186>a| c201>t|g258>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|g303>a|c309>t| a320>g| 319 (320) 225 (226) 213 12 (13) 5 (6) 7 1 (2) 3 4 0 1 0 0 0 2 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 1 0 0 0 0 1 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 22 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 117 114 108 6 4 2 0 1 3 0 0 0 0 0 2 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtcagtactgg atgtactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attaatggtgatggaagtagcaca agctatgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtatattattgt gcaagaggccgtatagtatcagttgggatgtcaggctggttcgacccc ARGRIVSVGMSGWFDP IGHV3-74 IGHD6-13 IGHJ5 ggattcaccttcagtcagtactgg atgtactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attaatggtgatggaagtagcaca agctatgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaccctgtatctgcaaatgaacagtctgagagtcgacgacacggctgtatattattgt gcaagaggccgtatagtatcagttgggatgtcaggctggttcgacccc unmatched 12 213 8 4 7 10 1 2 4 1 3 4 JY8QFUQ01ACMHQ 9666 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 908 91.63 197/215 nt NA Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD3-3*01 F 2 8 8 21 8.8.21 [0.17.38.11] CVRVGAPYYDFWSGYQTNWFDPW in-frame + ctggattcagcatcagtagttatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtctcatccattagtgcaagtactacttccatacattatgcagactcagtgaagggccgattcaccatctccagagacgacgccaagagttccctgtatttgcaaatgaacagcctgagagccgaggacacggctgtatattactgtgtaagagtgggggcgccatattacgatttctggagtggctatcagactaactggttcgacccctggggccagggaactctggtcaccgttacctcagcatccccgaccagccccaaggtcttcccgctgacgcctctgcagcaccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccccag NA unmatched, IGA1 64 79 [309] c86>g,T29>S(+ - +)|t88>a,F30>I(+ - -)|c108>t|a172>g,S58>A(- + -)|g173>c,S58>A(- + -)|t174>a,S58>A(- + -)|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|a191>c,Y64>S(+ - -)|t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|a244>g,N82>D(+ + -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a258>c|c265>t|g303>a|c314>t,A105>V(+ - +)|g315>a,A105>V(+ - +)| c86>g,T29>S(+ - +)|t88>a,F30>I(+ - -)|c108>t| a172>g,S58>A(- + -)|g173>c,S58>A(- + -)|t174>a,S58>A(- + -)|g185>c,S62>T(+ - +)|g188>c,S63>T(+ - +)|a191>c,Y64>S(+ - -)| t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|a244>g,N82>D(+ + -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a258>c|c265>t|g303>a| c314>t,A105>V(+ - +)|g315>a,A105>V(+ - +)|a320>t| 319 (320) 222 (223) 202 20 (21) 5 (6) 15 3 2 6 1 2 0 0 (1) 2 3 1 0 0 78 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 1 0 0 0 0 1 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 18 6 0 6 1 0 0 0 1 0 0 1 3 0 0 0 117 114 105 9 4 5 2 1 4 1 1 0 0 0 0 0 0 0 7 (8) 7 5 2 (3) 0 (1) 2 0 1 1 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ct ggattcagcatcagtagttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtgcaagtactacttccata cattatgcagactcagtgaagggccgattcaccatctccagagacgacgccaagagttccctgtatttgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gtaagagtgggggcgccatattacgatttctggagtggctatcagactaactggttcgacccc VRVGAPYYDFWSGYQTNWFDP IGHV3-21 IGHD3-3 IGHJ5 ggattcagcatcagtagttatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtctcatcc attagtgcaagtactacttccata cattatgcagactcagtgaagggccgattcaccatctccagagacgacgccaagagttccctgtatttgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gtaagagtgggggcgccatattacgatttctggagtggctatcagactaactggttcgacccc unmatched 18 213 10 8 6 10 4 8 4 1 5 8 JY8QFUQ01AD6VN 11552 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-3*01 F 3 8 8 11 8.8.11 [1.17.38.11] CARFGPLTRPDYW in-frame + ctctggattcacctttaccaactatggcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtggtgataccacataccacgcagactccgtgcagggccgattcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagtcgaggacacggccgtttattactgtgcgagattcggcccacttactcgacctgactactggggccagggaactttggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [281, 282] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)|c164>g,A55>G(- + -)|g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)|t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|g225>a|t246>c|g258>t|c279>t|c287>t,A96>V(+ - +)|a303>t| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)| c164>g,A55>G(- + -)| g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)| t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|g225>a|t246>c|g258>t|c279>t|c287>t,A96>V(+ - +)|a303>t| a317>g,K106>R(+ + +)|g319>t|a320>t| 316 (320) 221 (225) 207 (208) 14 (17) 5 (7) 9 (10) 0 (1) 3 2 2 1 0 1 (2) 0 2 2 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 117 114 106 8 5 3 0 1 2 2 1 0 1 0 0 0 1 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgataccaca taccacgcagactccgtgcagggccgattcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagtcgaggacacggccgtttattactgt gcgagattcggcccacttactcgacctgactac ARFGPLTRPDY IGHV3-23 IGHD3-3 IGHJ4 ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgataccaca taccacgcagactccgtgcagggccgattcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagtcgaggacacggccgtttattactgt gcgagattcggcccacttactcgacctgactac unmatched 14 213 7 7 5 10 2 4 5 0 4 5 JY8QFUQ01ADNPS 4354 productive Homsap IGHV3-7*01 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD2-15*01 F 2 8 8 15 8.8.15 [1.17.38.11] CARDPRSVSRYSLFDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcacctttagtgactattggatgagctgggtccgccaggctccagggaaggggctagagtgggtggccaacataaatagagatggaagtgagcaacactatgtggactctgtgaagggccgattcaccatctccagagacgacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacatggctatgtatttttgtgcgagagatcctcgttccgtgagcaggtactctttatttgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [293, 294] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g150>a|g171>t,K57>N(+ - -)|c172>a,Q58>R(+ - -)|a173>g,Q58>R(+ - -)|a193>c,K65>Q(+ - -)|t196>c,Y66>H(+ - -)|a244>g,N82>D(+ + -)|a254>g,N85>S(- - -)|c296>t,T99>M(- - -)|g301>a,V101>M(+ - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g150>a| g171>t,K57>N(+ - -)|c172>a,Q58>R(+ - -)|a173>g,Q58>R(+ - -)|a193>c,K65>Q(+ - -)| t196>c,Y66>H(+ - -)|a244>g,N82>D(+ + -)|a254>g,N85>S(- - -)|c296>t,T99>M(- - -)|g301>a,V101>M(+ - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| 320 225 211 14 1 13 4 3 2 1 1 1 1 0 0 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 0 0 1 1 0 0 0 0 1 0 117 114 107 7 0 7 2 1 2 1 0 0 1 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtgactattgg atgagctgggtccgccaggctccagggaaggggctagagtgggtggccaac ataaatagagatggaagtgagcaa cactatgtggactctgtgaagggccgattcaccatctccagagacgacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacatggctatgtatttttgt gcgagagatcctcgttccgtgagcaggtactctttatttgactcc ARDPRSVSRYSLFDS IGHV3-7 IGHD2-15 IGHJ4 ggattcacctttagtgactattgg atgagctgggtccgccaggctccagggaaggggctagagtgggtggccaac ataaatagagatggaagtgagcaa cactatgtggactctgtgaagggccgattcaccatctccagagacgacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacatggctatgtatttttgt gcgagagatcctcgttccgtgagcaggtactctttatttgactcc unmatched 14 213 10 4 5 7 5 7 1 0 7 6 JY8QFUQ01ADX4W 8877 productive (see comment) Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 1040 97.71 213/218 nt 97.25 212/218 nt Homsap IGHJ3*02 F 88 64 32/50 nt Homsap IGHD2-21*02 F 2 8 8 17 8.8.17 [1.17.38.11] CARPYCGGDCHNRGAFDIW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 61 to 61 in the user submitted sequence), (cause frameshift); cgtctggattcaccttcagtagctatggcatacactgggtccgccaggctccaggcaaggGggctggagtgggtggcagttatatggtatgatggaagtgaaatatactatgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgaggccatattgtggtggtgactgccacaatcgaggggcttttgatatctggggccaagggacaagtggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA unmatched, IGA2 57 89 [302, 303] g117>a,M39>I(+ + -)|a190>g,N64>E(+ - -)|t192>a,N64>E(+ - -)|a194>t,K65>I(- + -)|a225>c| g117>a,M39>I(+ + -)| a190>g,N64>E(+ - -)|t192>a,N64>E(+ - -)|a194>t,K65>I(- + -)| a225>c| a318>g|g319>c|a320>c| 317 (320) 223 (226) 218 5 (8) 1 (4) 4 1 (2) 1 0 0 1 (2) 0 1 1 0 (1) 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 1 1 0 0 0 0 117 114 113 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 5 (8) 5 5 0 (3) 0 (3) 0 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cgtct ggattcaccttcagtagctatggc atacactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatggtatgatggaagtgaaata tactatgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgaggccatattgtggtggtgactgccacaatcgaggggcttttgatatc ARPYCGGDCHNRGAFDI IGHV3-33 IGHD2-21 IGHJ3 ggattcaccttcagtagctatggc atacactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatggtatgatggaagtgaaata tactatgcagactccgtgaagggccgcttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgaggccatattgtggtggtgactgccacaatcgaggggcttttgatatc unmatched 5 213 2 3 1 1 1 4 1 0 1 3 JY8QFUQ01AEXEP 5531 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 931 92.59 200/216 nt 92.13 199/216 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-10*01 F 2 8 8 13 8.8.13 [1.16.38.11] CANWLSGTHSKFDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 1 nucleotides (from position 58 in the user submitted sequence), (cause frameshift) ctctggattcaccttcagcaagtatgccatgagctgggtccgccaggctccaggggagggctgcagtgggtctcagcaattagtggaaatggtgctgatatatactacgcagactccgtgaacggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgtgcgaattggctttcggggacacattctaagtttgactactggggcaagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttc NA unmatched, IGA2 57 89 [286, 287] t90>c|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a142>g|g151>c,E51>Q(+ + -)|t165>a|t174>a|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c192>t,S64>D(- - -)|c194>t,T65>I(- - -)|g216>c,K72>N(+ - -)|g261>a|t306>c| t90>c|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)| a142>g|g151>c,E51>Q(+ + -)|t165>a| t174>a|g176>a,S59>N(- - -)|g188>c,G63>A(- + -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c192>t,S64>D(- - -)|c194>t,T65>I(- - -)| g216>c,K72>N(+ - -)|g261>a|t306>c| a318>t,K106>N(+ - -)|g319>t|a320>g| 317 (320) 221 (224) 205 16 (19) 6 (8) 10 (11) 2 (3) 4 2 2 0 0 0 (1) 2 3 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 0 1 0 0 0 0 0 1 0 0 51 50 47 3 2 1 1 0 0 0 0 0 0 1 1 0 0 0 30 24 17 7 1 6 1 2 2 0 0 0 0 1 1 0 0 0 117 114 111 3 2 1 0 1 0 1 0 0 0 0 1 0 0 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagcaagtatgcc atgagctgggtccgccaggctccaggggagggctgcagtgggtctcagca attagtggaaatggtgctgatata tactacgcagactccgtgaacggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgt gcgaattggctttcggggacacattctaagtttgactac ANWLSGTHSKFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcaccttcagcaagtatgcc atgagctgggtccgccaggctccaggggagggctgcagtgggtctcagca attagtggaaatggtgctgatata tactacgcagactccgtgaacggccggttcaccatctccagagacaattccaagaacacgctatatctgcaaatgaacagcctgagagccgaggacacggccgtatactactgt gcgaattggctttcggggacacattctaagtttgactac unmatched 16 212 10 6 6 10 4 6 4 2 2 8 JY8QFUQ01AFU74 7032 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 903 91.59 196/214 nt NA Homsap IGHJ3*02 F 214 92 46/50 nt Homsap IGHD2-21*01 F 2 8 7 15 8.7.15 [1.17.38.11] CTRELRLAAARDGVDIW in-frame + ttctgggttcaccgtcagtagcaagttcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaattacttatcctgatggtactacatattatggagactccgtgaagggccgattcaccatctccagagacaattccaagaacacactggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgtacgagagaacttaggctggctgctgcgcgtgatggtgttgacatctggggccaggggacaatggtcaccgtctcttcagcatcccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 84 [289, 290] c75>t|c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)|g163>a,V55>I(+ - +)|t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|c198>t|c201>t|c203>g,A68>G(- + -)|g258>a|t262>g,Y88>D(- - -)|g303>t|c309>t|g313>a,A105>T(- - -)| c75>t| c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)| g163>a,V55>I(+ - +)| t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)| c198>t|c201>t|c203>g,A68>G(- + -)|g258>a|t262>g,Y88>D(- - -)|g303>t|c309>t| g313>a,A105>T(- - -)| 320 222 203 19 6 13 0 4 6 1 1 0 1 0 2 2 1 1 78 4 3 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 21 14 7 0 7 0 1 2 1 1 0 0 0 2 0 0 0 117 114 107 7 5 2 0 1 3 0 0 0 0 0 0 1 1 1 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ttct gggttcaccgtcagtagcaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaatt acttatcctgatggtactaca tattatggagactccgtgaagggccgattcaccatctccagagacaattccaagaacacactggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttaggctggctgctgcgcgtgatggtgttgacatc TRELRLAAARDGVDI IGHV3-53 IGHD2-21 IGHJ3 gggttcaccgtcagtagcaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaatt acttatcctgatggtactaca tattatggagactccgtgaagggccgattcaccatctccagagacaattccaagaacacactggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttaggctggctgctgcgcgtgatggtgttgacatc unmatched 17 210 9 8 8 13 1 4 5 0 3 9 JY8QFUQ01AG0BX 6003 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-2*01 F 3 8 8 11 8.8.11 [1.17.38.11] CARFGPLTRPDYW in-frame + ctctggattcacctttaccaactatggcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtggtgatagaacataccacgcagactccgtgcagggccggttcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagccgacgacgcgggcgtatattactgtgcgagattcggaccacttactcgacctgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtctcccctcggcgccctgctccaggcagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA unmatched, IGG2 79 62 [282] g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)|c164>g,A55>G(- + -)|g188>a,G63>D(- - -)|c192>a,S64>R(- - -)|t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|t246>c|g258>t|c279>t|g291>c,E97>D(+ - +)|a295>g,T99>A(- - -)|c299>g,A100>G(- + -)| g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)| c164>g,A55>G(- + -)| g188>a,G63>D(- - -)|c192>a,S64>R(- - -)| t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|t246>c|g258>t|c279>t|g291>c,E97>D(+ - +)|a295>g,T99>A(- - -)|c299>g,A100>G(- + -)| a317>g,K106>R(+ + +)|g319>t|a320>t| 316 (320) 221 (225) 207 (208) 14 (17) 3 (5) 11 (12) 1 (2) 2 1 2 1 1 0 (1) 0 2 3 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 22 2 0 2 0 1 0 0 0 1 0 0 0 0 0 0 117 114 106 8 3 5 1 0 1 2 1 0 0 0 1 1 1 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgatagaaca taccacgcagactccgtgcagggccggttcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagccgacgacgcgggcgtatattactgt gcgagattcggaccacttactcgacctgactac ARFGPLTRPDY IGHV3-23 IGHD2-2 IGHJ4 ggattcacctttaccaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgatagaaca taccacgcagactccgtgcagggccggttcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagccgacgacgcgggcgtatattactgt gcgagattcggaccacttactcgacctgactac unmatched 14 213 6 8 3 10 3 4 3 0 6 5 JY8QFUQ01AGTXI 14347 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 932 91.74 200/218 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD3-10*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKKYHYGSGTYLYYFDYW in-frame + cctctggattcacctttagccactttgccgtgacctgggtccgccaggctccagggaagggtctggaatgggtctcaactattagcggtagtgatggtagcaagtactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacaccctatatctgcaaatgaccagcctgagagccgaggacacggccgtatatttctgcgcgaagaagtatcactatggttcggggacttacttgtactattttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [300, 301] a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a110>t,Y37>F(- + -)|a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|g147>t|g153>a|g163>a,A55>T(- - -)|t171>c|g185>a,G62>D(- - -)|c194>a,T65>K(- - -)|a195>g,T65>K(- - -)|a215>g,K72>R(+ + +)|g258>c|g261>a|a275>c,N92>T(- + -)|a308>t,Y103>F(- + -)|t312>c| a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a110>t,Y37>F(- + -)| a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|g147>t|g153>a|g163>a,A55>T(- - -)| t171>c|g185>a,G62>D(- - -)|c194>a,T65>K(- - -)|a195>g,T65>K(- - -)| a215>g,K72>R(+ + +)|g258>c|g261>a|a275>c,N92>T(- + -)|a308>t,Y103>F(- + -)|t312>c| a318>g|g319>a| 317 (320) 223 (226) 205 (206) 18 (20) 6 (8) 12 3 (4) 5 (6) 0 2 2 1 2 0 2 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 1 0 1 0 0 0 0 0 51 51 46 5 2 3 1 2 0 0 0 0 0 0 1 0 1 0 30 24 20 4 1 3 1 1 0 1 0 1 0 0 0 0 0 0 117 114 108 6 3 3 1 1 0 1 1 0 1 0 1 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagccactttgcc gtgacctgggtccgccaggctccagggaagggtctggaatgggtctcaact attagcggtagtgatggtagcaag tactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacaccctatatctgcaaatgaccagcctgagagccgaggacacggccgtatatttctgc gcgaagaagtatcactatggttcggggacttacttgtactattttgactac AKKYHYGSGTYLYYFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcacctttagccactttgcc gtgacctgggtccgccaggctccagggaagggtctggaatgggtctcaact attagcggtagtgatggtagcaag tactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacaccctatatctgcaaatgaccagcctgagagccgaggacacggccgtatatttctgc gcgaagaagtatcactatggttcggggacttacttgtactattttgactac unmatched 18 213 10 8 5 9 5 9 5 1 6 6 JY8QFUQ01AHQN6 16004 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F (see comment) 771 83.64 184/220 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-22*01 F 2 10 7 20 10.7.20 [1.17.38.11] CARQSEPANNFNDPSRYFFDTW in-frame + Low V-REGION identity (83,64% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctcccgtgtctccatttccattaatgattactactggggctggatccgccagcccccaggaaagccgctggagtggattgggactgtccattcccttgggtacaattacaacaacccgtccctcaagagtcgactcaccatttccgcagacacgtccaggaatcagatctccctgaaactgacgtctgtgaccgccgcagacacggctgtctatttctgtgcgagacagagcgagcccgccaataacttcaatgacccaagtcgttatttctttgacacttggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [311, 312] t78>c|g79>c,G27>R(- - -)|g83>t,G28>V(- - -)|c90>t|a91>t|g92>c|g101>t,S34>I(- - -)|g104>a,S35>N(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g141>a|g145>c,G49>P(+ - -)|g146>c,G49>P(+ - -)|g164>c,S55>T(+ - +)|a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|a173>c,Y58>S(+ - -)|t174>c,Y58>S(+ - -)|a175>c,S59>L(- - -)|g176>t,S59>L(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|c194>a,T65>N(- + -)|c195>t,T65>N(- + -)|t199>a,Y67>N(- - -)|g226>c,V76>L(+ - +)|a234>t|t239>c,V80>A(+ - +)|a251>g,K84>R(+ + +)|c255>t|t259>a,F87>I(+ - -)|g270>a|g275>c,S92>T(+ - +)|c276>g,S92>T(+ - +)|g303>c|a308>t,Y103>F(- + -)| t78>c| g79>c,G27>R(- - -)|g83>t,G28>V(- - -)|c90>t|a91>t|g92>c|g101>t,S34>I(- - -)|g104>a,S35>N(- - -)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g141>a|g145>c,G49>P(+ - -)|g146>c,G49>P(+ - -)|g164>c,S55>T(+ - +)| a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|a173>c,Y58>S(+ - -)|t174>c,Y58>S(+ - -)|a175>c,S59>L(- - -)|g176>t,S59>L(- - -)|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|c194>a,T65>N(- + -)|c195>t,T65>N(- + -)| t199>a,Y67>N(- - -)|g226>c,V76>L(+ - +)|a234>t|t239>c,V80>A(+ - +)|a251>g,K84>R(+ + +)|c255>t|t259>a,F87>I(+ - -)|g270>a|g275>c,S92>T(+ - +)|c276>g,S92>T(+ - +)|g303>c|a308>t,Y103>F(- + -)| 320 228 192 36 9 27 3 5 3 4 2 1 4 2 8 1 3 0 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 30 21 9 3 6 1 2 1 0 0 0 1 0 2 0 2 0 51 51 47 4 1 3 0 1 0 0 0 0 0 0 3 0 0 0 30 21 11 10 0 10 1 1 1 2 2 1 1 0 0 0 1 0 117 114 102 12 4 8 1 1 1 1 0 0 2 2 3 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctcc cgtgtctccatttccattaatgattactac tggggctggatccgccagcccccaggaaagccgctggagtggattgggact gtccattcccttgggtacaat tacaacaacccgtccctcaagagtcgactcaccatttccgcagacacgtccaggaatcagatctccctgaaactgacgtctgtgaccgccgcagacacggctgtctatttctgt gcgagacagagcgagcccgccaataacttcaatgacccaagtcgttatttctttgacact ARQSEPANNFNDPSRYFFDT IGHV4-39 IGHD3-22 IGHJ4 cgtgtctccatttccattaatgattactac tggggctggatccgccagcccccaggaaagccgctggagtggattgggact gtccattcccttgggtacaat tacaacaacccgtccctcaagagtcgactcaccatttccgcagacacgtccaggaatcagatctccctgaaactgacgtctgtgaccgccgcagacacggctgtctatttctgt gcgagacagagcgagcccgccaataacttcaatgacccaagtcgttatttctttgacact unmatched 35 216 14 21 8 21 6 14 5 3 11 16 JY8QFUQ01AIE5M 10927 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 1080 100 217/217 nt NA Homsap IGHJ5*02 F 255 100 51/51 nt Homsap IGHD2-2*01 F 3 8 8 18 8.8.18 [1.17.38.11] CAKFMGVVVVPAAHNWFDPW in-frame + ctctggattcacctttagcagctatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtctcagctattagtggtagtggtggtagcacatactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaaattcatgggagttgtagtagtaccagctgcccacaactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgcctgctccaggagcaccctccgagagcacagcggccctgggctgcctggtcaaggactcacttccccgaaccgtgacggtgtcgtggaactcagg NA unmatched, IGG2 57 75 [303] g319>t|a320>t| 318 (320) 223 (225) 223 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcagctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagct attagtggtagtggtggtagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaattcatgggagttgtagtagtaccagctgcccacaactggttcgacccc AKFMGVVVVPAAHNWFDP IGHV3-23 IGHD2-2 IGHJ5 ggattcacctttagcagctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagct attagtggtagtggtggtagcaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaaattcatgggagttgtagtagtaccagctgcccacaactggttcgacccc unmatched 0 213 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01AIYO5 18163 productive Homsap IGHV3-7*01 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F (see comment) 213 93.75 45/48 nt Homsap IGHD2-15*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARVRGYYGGQYFDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcacctttagtagttattggatgacctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaaggaagatgggaatgataaatactatgtcgactctgtgaggggccggttcaccatctccagagacaacgccaagagctcactgtttctgcaagtgaacagcctgagagccgacgacacggctgtttattactgtgcgagagttcgaggttattacggtgggcagtactttgattcctggggccagggaaccctggtcaccgtctcctcagcatcccccgaccancccctcaaggtttcccgctgagcctctgccagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA unmatched, IGA1 86 68 [293, 292] c108>t|g119>c,S40>T(+ - +)|c172>g,Q58>E(+ + -)|a186>g|g188>a,S63>N(- - -)|g192>t,E64>D(+ - +)|g204>c|a215>g,K72>R(+ + +)|a225>g|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|a271>g,M91>V(+ - -)|g291>c,E97>D(+ - +)|g303>t| c108>t| g119>c,S40>T(+ - +)| c172>g,Q58>E(+ + -)|a186>g|g188>a,S63>N(- - -)|g192>t,E64>D(+ - +)| g204>c|a215>g,K72>R(+ + +)|a225>g|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|a271>g,M91>V(+ - -)|g291>c,E97>D(+ - +)|g303>t| a320>t| 319 (320) 224 (225) 210 14 (15) 5 (6) 9 5 1 1 0 0 0 1 (2) 0 3 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 20 4 1 3 1 1 0 0 0 0 0 0 0 1 1 0 117 114 106 8 3 5 4 0 0 0 0 0 1 0 2 0 1 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtagttattgg atgacctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatgggaatgataaa tactatgtcgactctgtgaggggccggttcaccatctccagagacaacgccaagagctcactgtttctgcaagtgaacagcctgagagccgacgacacggctgtttattactgt gcgagagttcgaggttattacggtgggcagtactttgattcc ARVRGYYGGQYFDS IGHV3-7 IGHD2-15 IGHJ4 ggattcacctttagtagttattgg atgacctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatgggaatgataaa tactatgtcgactctgtgaggggccggttcaccatctccagagacaacgccaagagctcactgtttctgcaagtgaacagcctgagagccgacgacacggctgtttattactgt gcgagagttcgaggttattacggtgggcagtactttgattcc unmatched 14 213 7 7 2 8 5 6 3 2 6 3 JY8QFUQ01AJFOA 12085 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 873 89.4 194/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-10*01 F 3 8 8 10 8.8.10 [1.17.38.11] CSKDTFGREDYW in-frame + ctctggattctccgtcagtaattactggatgcactgggtccgccaggctccaggggaggggctggagtgggtctcacgtattaatgaagatggtagtcggacagactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgtagtaaagacacatttgggcgagaagactactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [278] a85>t,T29>S(+ - +)|t88>g,F30>V(+ - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a132>g|a142>g,K48>E(+ - -)|t152>a,V51>E(- + -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>t|a190>c,S64>R(- - -)|c192>g,S64>R(- - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c234>t|c236>a,S79>Y(+ - -)|a245>g,N82>S(- - -)|g258>a|c287>t,A96>V(+ - +)|g291>a|g303>c|c309>t| a85>t,T29>S(+ - +)|t88>g,F30>V(+ - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a132>g|a142>g,K48>E(+ - -)|t152>a,V51>E(- + -)| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>t|a190>c,S64>R(- - -)|c192>g,S64>R(- - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c234>t|c236>a,S79>Y(+ - -)|a245>g,N82>S(- - -)|g258>a|c287>t,A96>V(+ - +)|g291>a|g303>c|c309>t| g313>a,A105>S(- + -)|c314>g,A105>S(- + -)|a315>t,A105>S(- + -)|g317>a,R106>K(+ + +)| 320 225 198 27 7 20 5 7 4 0 1 1 2 2 1 2 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 1 0 0 0 1 0 0 0 0 1 51 51 48 3 1 2 2 0 0 0 0 0 0 1 0 0 0 0 30 24 18 6 1 5 1 1 0 0 1 0 0 1 0 1 1 0 117 114 104 10 5 5 2 3 3 0 0 1 0 0 1 0 0 0 8 8 4 4 0 4 0 2 0 0 0 0 1 0 0 1 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattctccgtcagtaattactgg atgcactgggtccgccaggctccaggggaggggctggagtgggtctcacgt attaatgaagatggtagtcggaca gactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgt agtaaagacacatttgggcgagaagactac SKDTFGREDY IGHV3-74 IGHD3-10 IGHJ4 ggattctccgtcagtaattactgg atgcactgggtccgccaggctccaggggaggggctggagtgggtctcacgt attaatgaagatggtagtcggaca gactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgt agtaaagacacatttgggcgagaagactac unmatched 23 213 14 9 9 13 5 10 6 1 7 9 JY8QFUQ01AJTH3 5198 productive Homsap IGHV4-39*02 F 951 92.73 204/220 nt NA Homsap IGHJ4*02 F 141 77.08 37/48 nt Homsap IGHD4-17*01 F 3 10 7 12 10.7.12 [1.17.38.11] CASVTTVSMRMKYW in-frame + ctctggtggctccatcagcagtgatagttactactggggctggatccgccagtccccagggaaggggctggagtggattgggaatatctattatcgtgggagcacctattacaacccatccctcgagagtcggctcaccatgtcggtagacacgtccaggaacctcttctccctgaggctgagctctgtgaccgccgcagacacggctgtatattactgtgcgagcgtgactacggtgtctatgaggatgaaatactggggccggggaaccccggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA unmatched, IGA2 64 100 [287, 288] a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c133>t,P45>S(+ - -)|g164>a,S55>N(- - -)|a175>c,S59>R(- - -)|c198>t|g207>a|a214>g,K72>E(+ - -)|a225>g|g226>c,V76>L(+ - +)|a234>g,I78>M(+ + -)|c237>g|a251>g,K84>R(+ + +)|a257>t,H86>L(- - -)|a269>g,K90>R(+ + +)|g303>a| a103>g,S35>D(- - -)|g104>a,S35>D(- - -)| c133>t,P45>S(+ - -)|g164>a,S55>N(- - -)| a175>c,S59>R(- - -)| c198>t|g207>a|a214>g,K72>E(+ - -)|a225>g|g226>c,V76>L(+ - +)|a234>g,I78>M(+ + -)|c237>g|a251>g,K84>R(+ + +)|a257>t,H86>L(- - -)|a269>g,K90>R(+ + +)|g303>a| a318>c,R106>S(- - -)|a320>t| 317 (320) 225 (228) 209 (210) 16 (18) 5 (6) 11 (12) 6 4 2 0 1 (2) 0 1 (2) 0 1 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 30 21 20 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 117 114 103 11 5 6 5 2 1 0 0 0 1 0 1 1 0 0 5 (8) 5 5 (6) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtggctccatcagcagtgatagttactac tggggctggatccgccagtccccagggaaggggctggagtggattgggaat atctattatcgtgggagcacc tattacaacccatccctcgagagtcggctcaccatgtcggtagacacgtccaggaacctcttctccctgaggctgagctctgtgaccgccgcagacacggctgtatattactgt gcgagcgtgactacggtgtctatgaggatgaaatac ASVTTVSMRMKY IGHV4-39 IGHD4-17 IGHJ4 ggtggctccatcagcagtgatagttactac tggggctggatccgccagtccccagggaaggggctggagtggattgggaat atctattatcgtgggagcacc tattacaacccatccctcgagagtcggctcaccatgtcggtagacacgtccaggaacctcttctccctgaggctgagctctgtgaccgccgcagacacggctgtatattactgt gcgagcgtgactacggtgtctatgaggatgaaatac unmatched 16 216 12 4 6 8 6 8 5 0 8 3 JY8QFUQ01AMBVG 16774 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 819 86.64 188/217 nt NA Homsap IGHJ4*02 F 105 68.75 33/48 nt Homsap IGHD2-15*01 F 2 8 8 19 8.8.19 [1.17.38.11] CAKDYGDCRGGRCYAEPLDYW in-frame + ctctggattcagcctcgccacttatagtatgagttgggtccgccaggctccaggaaaggggctggagtgggtctcaggtattagtgatcatggtattgacatatactatgcagactccgtgaggggccggtttaccatctccagagacatttccaagaacacggtgtatctacaaatgaacagcctgggagtcgaggacacggccgtatattactgtgcgaaagattacggggactgtcgtggtggtagatgctacgccgagcccctcgactactggggccagggaaccctggtcacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagccacccagccaggatgggaagcntggtccatgcgctgcctgggtccaggcttcttcccc NA unmatched, IGA1 50 58 [304] c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|t90>c,F30>L(+ - -)|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>a,A38>S(- + -)|c113>g,A38>S(- + -)|c114>t,A38>S(- + -)|c120>t|g141>a|c164>g,A55>G(- + -)|g173>a,G58>D(- - -)|a175>c,S59>H(+ - -)|g176>a,S59>H(+ - -)|g187>a,G63>I(- - -)|g188>t,G63>I(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>t,T65>I(- - -)|c201>t|a215>g,K72>R(+ + +)|c228>t|a245>t,N82>I(- - -)|c259>g,L87>V(+ - +)|g267>a|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)| c86>g,T29>S(+ - +)|t88>c,F30>L(+ - -)|t90>c,F30>L(+ - -)|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g112>a,A38>S(- + -)|c113>g,A38>S(- + -)|c114>t,A38>S(- + -)| c120>t|g141>a|c164>g,A55>G(- + -)| g173>a,G58>D(- - -)|a175>c,S59>H(+ - -)|g176>a,S59>H(+ - -)|g187>a,G63>I(- - -)|g188>t,G63>I(- - -)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>t,T65>I(- - -)| c201>t|a215>g,K72>R(+ + +)|c228>t|a245>t,N82>I(- - -)|c259>g,L87>V(+ - +)|g267>a|a283>g,R95>G(- - -)|c287>t,A96>V(+ - +)| 320 225 196 29 5 24 4 7 7 2 1 0 1 0 2 4 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 14 10 0 10 1 1 2 2 0 0 0 0 2 2 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 0 0 1 0 0 30 24 16 8 0 8 1 4 1 0 1 0 0 0 0 0 1 0 117 114 106 8 3 5 2 1 3 0 0 0 1 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagcctcgccacttatagt atgagttgggtccgccaggctccaggaaaggggctggagtgggtctcaggt attagtgatcatggtattgacata tactatgcagactccgtgaggggccggtttaccatctccagagacatttccaagaacacggtgtatctacaaatgaacagcctgggagtcgaggacacggccgtatattactgt gcgaaagattacggggactgtcgtggtggtagatgctacgccgagcccctcgactac AKDYGDCRGGRCYAEPLDY IGHV3-23 IGHD2-15 IGHJ4 ggattcagcctcgccacttatagt atgagttgggtccgccaggctccaggaaaggggctggagtgggtctcaggt attagtgatcatggtattgacata tactatgcagactccgtgaggggccggtttaccatctccagagacatttccaagaacacggtgtatctacaaatgaacagcctgggagtcgaggacacggccgtatattactgt gcgaaagattacggggactgtcgtggtggtagatgctacgccgagcccctcgactac unmatched 29 213 20 9 14 21 6 8 5 0 6 18 JY8QFUQ01AMG29 14619 productive (see comment) Homsap IGHV3-48*02 F, or Homsap IGHV3-48*04 F 939 93.46 200/214 nt 92.99 199/214 nt Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD5-18*01 F 3 8 8 13 8.8.13 [0.17.38.11] CARDRRGYSYEMDYW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 52 of V-REGION: 'G' (1 nucleotides at position 66 to 66 in the user submitted sequence), (cause frameshift); tggattcaccttcagtgtccatagcatgaactgggtccgccaggctccagggaaggggctggagtGgggtttcataccttagtagtggtagtgataccatatactacgcagactctgtgaggggccggttcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagtggcctgagagacgaggacacggctgtttattactgtgcgagagatcggcgtgggtatagttatgaaatggactactggggacagggaaccctggtcaccgtctcctcagcctcctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGA1 0 0 [] a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|t109>c,Y37>H(+ - -)|a166>c,I56>L(+ + +)|a175>g,S59>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a215>g,K72>R(+ + +)|a225>g|t246>c|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|a277>g,S93>G(+ + -)|g303>t| a106>g,S36>V(- - -)|g107>t,S36>V(- - -)|t109>c,Y37>H(+ - -)| a166>c,I56>L(+ + +)|a175>g,S59>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)| a215>g,K72>R(+ + +)|a225>g|t246>c|a275>g,N92>S(- - -)|c276>t,N92>S(- - -)|a277>g,S93>G(+ + -)|g303>t| 320 222 208 14 3 11 7 1 1 2 1 0 0 0 0 0 2 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 0 0 1 0 0 0 0 0 0 1 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 2 1 0 0 1 0 0 0 0 0 0 0 117 114 107 7 3 4 4 0 1 1 0 0 0 0 0 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggattcaccttcagtgtccatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac cttagtagtggtagtgataccata tactacgcagactctgtgaggggccggttcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagtggcctgagagacgaggacacggctgtttattactgt gcgagagatcggcgtgggtatagttatgaaatggactac ARDRRGYSYEMDY IGHV3-48 IGHD5-18 IGHJ4 ggattcaccttcagtgtccatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac cttagtagtggtagtgataccata tactacgcagactctgtgaggggccggttcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagtggcctgagagacgaggacacggctgtttattactgt gcgagagatcggcgtgggtatagttatgaaatggactac unmatched 14 213 11 3 2 4 9 10 3 0 4 7 JY8QFUQ01AMV93 14634 productive Homsap IGHV3-7*01 F 950 93.12 203/218 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-12*01 F 3 8 8 10 8.8.10 [1.17.38.11] CATISDYRFEFW in-frame + cctctggattcacctttagtggctattggatgagttgggtccgccaggctccggggaagggtctggagtgggtggccaacatagagaaagatggaagtgacataaagtatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaattcactgtctctgcaaatgaacagcctgagagccgacgacacggctatttattactgtgcgaccattagtgactaccgatttgaattctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA unmatched, IGA2 64 100 [279, 280] a106>g,S36>G(+ + -)|c120>t|a138>g|g147>t|a169>g,K57>E(+ - -)|c172>a,Q58>K(+ - -)|g192>c,E64>D(+ - +)|a194>t,K65>I(- + -)|t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|c255>t|a263>c,Y88>S(+ - -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| a106>g,S36>G(+ + -)| c120>t|a138>g|g147>t| a169>g,K57>E(+ - -)|c172>a,Q58>K(+ - -)|g192>c,E64>D(+ - +)|a194>t,K65>I(- + -)| t196>a,Y66>K(- - -)|c198>g,Y66>K(- - -)|c255>t|a263>c,Y88>S(+ - -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| g317>c,R106>T(- - -)|a318>c,R106>T(- - -)|g319>a|a320>t| 316 (320) 222 (226) 207 15 (19) 4 (6) 11 (13) 3 1 (2) 2 0 1 (2) 1 1 (2) 1 2 (3) 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 51 51 48 3 3 0 1 0 1 0 0 0 0 0 0 0 1 0 30 24 20 4 0 4 1 0 0 0 0 1 1 0 1 0 0 0 117 114 107 7 1 6 0 1 1 0 1 0 0 1 1 1 1 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 0 (1) 0 0 0 (1) 0 0 (1) 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagtggctattgg atgagttgggtccgccaggctccggggaagggtctggagtgggtggccaac atagagaaagatggaagtgacata aagtatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaattcactgtctctgcaaatgaacagcctgagagccgacgacacggctatttattactgt gcgaccattagtgactaccgatttgaattc ATISDYRFEF IGHV3-7 IGHD5-12 IGHJ4 ggattcacctttagtggctattgg atgagttgggtccgccaggctccggggaagggtctggagtgggtggccaac atagagaaagatggaagtgacata aagtatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaattcactgtctctgcaaatgaacagcctgagagccgacgacacggctatttattactgt gcgaccattagtgactaccgatttgaattc unmatched 15 213 6 9 3 9 3 6 4 0 6 5 JY8QFUQ01ANFJ6 11724 productive (see comment) Homsap IGHV4-59*03 F 940 93.06 201/216 nt 92.59 200/216 nt Homsap IGHJ5*02 F (see comment) 201 88.24 45/51 nt Homsap IGHD3-22*01 F 2 8 7 16 8.7.16 [2.17.38.11] CARGHFDSSGYSNPLDHW in-frame - Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in CDR2-IMGT at codon 59 of V-REGION: 'AGCGGG' (6 nucleotides at position 91 to 96 in the user submitted sequence), (do not cause frameshift); gtctctggtgtctccatcagtagtttctactggagttggatgaggcagcccccagggaggggactggagtggattggatatgtccatggcAGCGGGagtgggagcaccaactccaacccctccctcaagagtcgagtcaccatgtcagtggacacgtccaagaaccaattctccctgaagctgggctctgtgaccgctgcggacacggccgtgtattactgtgcgagaggacattttgatagtagtggttattccaaccctttagaccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccctcaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [301] g83>t,G28>V(- - -)|a110>t,Y37>F(- + -)|c120>t|c126>g,I42>M(+ + -)|c127>a|a143>g,K48>R(+ + +)|g162>a|a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|t172>g,Y58>G(+ - -)|a173>g,Y58>G(+ - -)|a200>c,Y67>S(+ - -)|a234>g,I78>M(+ + -)|a240>g|a274>g,S92>G(+ + -)| g83>t,G28>V(- - -)|a110>t,Y37>F(- + -)| c120>t|c126>g,I42>M(+ + -)|c127>a|a143>g,K48>R(+ + +)|g162>a| a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|t172>g,Y58>G(+ - -)|a173>g,Y58>G(+ - -)| a200>c,Y67>S(+ - -)|a234>g,I78>M(+ + -)|a240>g|a274>g,S92>G(+ + -)| 315 219 204 15 4 11 6 1 1 1 1 1 1 0 0 1 1 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 0 0 1 0 51 51 46 5 3 2 1 1 1 0 0 1 0 0 0 1 0 0 30 21 17 4 0 4 2 0 0 1 0 0 0 0 0 0 0 1 117 114 110 4 1 3 3 0 0 0 1 0 0 0 0 0 0 0 3 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,257-258(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| gtctct ggtgtctccatcagtagtttctac tggagttggatgaggcagcccccagggaggggactggagtggattggatat gtccatggcagtgggagcacc aactccaacccctccctcaagagtcgagtcaccatgtcagtggacacgtccaagaaccaattctccctgaagctgggctctgtgaccgctgcggacacggccgtgtattactgt gcgagaggacattttgatagtagtggttattccaaccctttagaccac ARGHFDSSGYSNPLDH IGHV4-59 IGHD3-22 IGHJ5 ggtgtctccatcagtagtttctac tggagttggatgaggcagcccccagggaggggactggagtggattggatat gtccatggcagtgggagcacc aactccaacccctccctcaagagtcgagtcaccatgtcagtggacacgtccaagaaccaattctccctgaagctgggctctgtgaccgctgcggacacggccgtgtattactgt gcgagaggacattttgatagtagtggttattccaaccctttagaccac unmatched 15 210 9 6 2 5 7 10 4 0 5 6 JY8QFUQ01ANN07 4265 productive (see comment) Homsap IGHV4-4*07 F 844 88.73 189/213 nt 87.32 186/213 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-19*01 F 1 8 7 11 8.7.11 [1.17.37.11] CARSGWDNQFDFW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'TGGCAC' (6 nucleotides at position 22 to 27 in the user submitted sequence), (do not cause frameshift);in FR3-IMGT at codon 103 of V-REGION: 'T' (1 nucleotides at position 214 to 214 in the user submitted sequence), (cause frameshift); in FR3-IMGT, from codon 68 of V-REGION: 1 nucleotides (from position 109 in the user submitted sequence), (cause frameshift) ctctggtgtcgccaccagtagTGGCACttactactggagctggatccggcagtccgccggggcgggactagagtggattgggcgcatctataccggtcacaccaccatttacaaccctccctcaagggtcgagtcaccatgtcacttgacatgtccaagaaccagatctccctgaggctgacctctgtgaccgccgcagatacggccgtgtatTtactgtgcgcgaagtggctgggacaatcaatttgacttctggggccagggaaccctggtcaccgtctcctcagcttccaccaagggcccatccgtcttccccctggcgccctgctcaggagcacctccgagancacagccgccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG4 50 62 [285, 277] g83>t,G28>V(- - -)|t85>g,S29>A(- + -)|t89>c,I30>T(- - -)|c133>t,P45>S(+ - -)|a142>g,K48>A(- - -)|a143>c,K48>A(- - -)|g150>a|t165>c|a175>g,S59>G(+ + -)|g187>c,G63>H(+ - -)|g188>a,G63>H(+ - -)|g189>c,G63>H(+ - -)|g191>c,S64>T(+ - +)|a197>t,N66>I(- - -)|c198>t,N66>I(- - -)|a220>g,S74>G(+ + -)|g238>c,V80>L(+ - +)|a240>t,V80>L(+ - +)|c245>t,T82>M(- - -)|t259>a,F87>I(+ - -)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|g291>a|c294>t|a316>c| g83>t,G28>V(- - -)|t85>g,S29>A(- + -)|t89>c,I30>T(- - -)| c133>t,P45>S(+ - -)|a142>g,K48>A(- - -)|a143>c,K48>A(- - -)|g150>a|t165>c| a175>g,S59>G(+ + -)|g187>c,G63>H(+ - -)|g188>a,G63>H(+ - -)|g189>c,G63>H(+ - -)|g191>c,S64>T(+ - +)| a197>t,N66>I(- - -)|c198>t,N66>I(- - -)|a220>g,S74>G(+ + -)|g238>c,V80>L(+ - +)|a240>t,V80>L(+ - +)|c245>t,T82>M(- - -)|t259>a,F87>I(+ - -)|a269>g,K90>R(+ + +)|g275>c,S92>T(+ - +)|g291>a|c294>t| a316>c|g319>a|a320>g| 318 (320) 219 (221) 194 25 (27) 5 (7) 20 4 (5) 3 (4) 4 2 2 0 2 1 5 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 1 0 0 0 0 0 0 1 1 51 51 46 5 2 3 1 1 1 1 1 0 0 0 0 0 0 0 30 21 16 5 0 5 1 1 0 0 0 0 0 0 3 0 0 0 117 113 102 11 2 9 2 1 3 0 0 0 2 1 2 0 0 0 6 (8) 6 5 1 (3) 1 (3) 0 0 (1) 0 (1) 0 0 1 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtgtcgccaccagtagttactac tggagctggatccggcagtccgccggggcgggactagagtggattgggcgc atctataccggtcacaccacc atttacaaccctccctcaagggtcgagtcaccatgtcacttgacatgtccaagaaccagatctccctgaggctgacctctgtgaccgccgcagatacggccgtgtattactgt gcgcgaagtggctgggacaatcaatttgacttc ARSGWDNQFDF IGHV4-4 IGHD6-19 IGHJ4 ggtgtcgccaccagtagttactac tggagctggatccggcagtccgccggggcgggactagagtggattgggcgc atctataccggtcacaccacc atttacaaccctccctcaagggtcgagtcaccatgtcacttgacatgtccaagaaccagatctccctgaggctgacctctgtgaccgccgcagatacggccgtgtattactgt gcgcgaagtggctgggacaatcaatttgacttc unmatched 24 209 13 11 7 13 6 11 4 0 12 8 JY8QFUQ01ANOBA 18469 productive (see comment) Homsap IGHV3-23*03 F, or Homsap IGHV3-23*05 F (see comment) 683 80 172/215 nt 79.53 171/215 nt Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD3-16*01 F 3 8 7 17 8.7.17 [1.17.38.11] CVRDAETTVSGVAGWFDPW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (80,00% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in CDR2-IMGT, from codon 64 of V-REGION: 3 nucleotides (from position 99 in the user submitted sequence), (do not cause frameshift) cctctggattcagctttagcgattttgccatgagttgggtccgccaacctccaggaaaggggctggagtgggtcgcaagtgttgacagaggtggcactacatactatgcaggctccatgaagggccggctcgccgtctctagagacgatgtcgacaagacagtgagtctgcagatgaacaatctgacagtcgaggacacggccacatatcactgtgtgagagacgccgagacgacggtgtcgggagtcgctggctggttcgacccctggggccagggaaccctggtcaccgtctccgcagcttccaccaagggcccatccgtcttcccccctggcgccctgctccaggagcacctccgacgagcacagccgccctgggctgcctggtcaaggactacttccccgaaccggctgacggtgtcgtggaactca NA unmatched, IGG4 64 75 [299, 298] c86>g,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|a110>t,Y37>F(- + -)|c120>t|g132>a|g133>c,A45>P(- - -)|g141>a|t160>g,S54>A(- + -)|g163>a,V55>S(- - -)|t164>g,V55>S(- - -)|a166>g,I56>V(+ - +)|t169>g,Y57>D(- - -)|t171>c,Y57>D(- - -)|c174>a,S58>R(- - -)|t186>c|g188>c,S63>T(+ - +)|a206>g,D69>G(- - -)|g211>a,V71>M(+ - -)|t226>c,F76>L(+ - -)|a229>g,T77>A(- - -)|a232>g,I78>V(+ - +)|c237>t|t243>c|a244>g,N82>D(+ + -)|t247>g,S83>V(- - -)|c248>t,S83>V(- - -)|a250>g,K84>D(+ - -)|g252>c,K84>D(+ - -)|c255>g,N85>K(+ - -)|g258>a|c259>g,L87>V(+ - +)|t262>a,Y88>S(+ - -)|a263>g,Y88>S(+ - -)|a270>g|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|t307>c,Y103>H(+ - -)|c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)| c86>g,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|a110>t,Y37>F(- + -)| c120>t|g132>a|g133>c,A45>P(- - -)|g141>a|t160>g,S54>A(- + -)|g163>a,V55>S(- - -)|t164>g,V55>S(- - -)| a166>g,I56>V(+ - +)|t169>g,Y57>D(- - -)|t171>c,Y57>D(- - -)|c174>a,S58>R(- - -)|t186>c|g188>c,S63>T(+ - +)| a206>g,D69>G(- - -)|g211>a,V71>M(+ - -)|t226>c,F76>L(+ - -)|a229>g,T77>A(- - -)|a232>g,I78>V(+ - +)|c237>t|t243>c|a244>g,N82>D(+ + -)|t247>g,S83>V(- - -)|c248>t,S83>V(- - -)|a250>g,K84>D(+ - -)|g252>c,K84>D(+ - -)|c255>g,N85>K(+ - -)|g258>a|c259>g,L87>V(+ - +)|t262>a,Y88>S(+ - -)|a263>g,Y88>S(+ - -)|a270>g|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|t307>c,Y103>H(+ - -)| c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)| 320 223 178 45 8 37 10 8 7 6 0 1 1 1 4 3 0 4 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 1 1 0 0 0 1 0 0 1 0 0 51 51 44 7 3 4 0 3 1 0 0 0 0 0 1 0 0 2 30 21 15 6 1 5 1 0 0 2 0 1 0 0 1 0 0 1 117 114 89 25 4 21 7 4 4 4 0 0 0 1 2 2 0 1 8 8 6 2 0 2 1 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|ta,243-244(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,243-244(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,184-187(CDR2)|agca,190-193(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcagctttagcgattttgcc atgagttgggtccgccaacctccaggaaaggggctggagtgggtcgcaagt gttgacagaggtggcactaca tactatgcaggctccatgaagggccggctcgccgtctctagagacgatgtcgacaagacagtgagtctgcagatgaacaatctgacagtcgaggacacggccacatatcactgt gtgagagacgccgagacgacggtgtcgggagtcgctggctggttcgacccc VRDAETTVSGVAGWFDP IGHV3-23 IGHD3-16 IGHJ5 ggattcagctttagcgattttgcc atgagttgggtccgccaacctccaggaaaggggctggagtgggtcgcaagt gttgacagaggtggcactaca tactatgcaggctccatgaagggccggctcgccgtctctagagacgatgtcgacaagacagtgagtctgcagatgaacaatctgacagtcgaggacacggccacatatcactgt gtgagagacgccgagacgacggtgtcgggagtcgctggctggttcgacccc unmatched 43 210 29 14 14 22 15 21 7 1 25 10 JY8QFUQ01ANT01 11238 productive (see comment) Homsap IGHV3-33*03 F 904 91.2 197/216 nt 90.74 196/216 nt Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD6-19*01 F 1 8 8 12 8.8.12 [1.17.37.11] CARAASHGWYLDSW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR3-IMGT, from codon 95 of V-REGION: 1 nucleotides (from position 190 in the user submitted sequence), (cause frameshift) gtctggattcatcttcagtggctatggcatgcactgggtccgccaggtcccaggcaagggcctggagtgggtggcggttattcggtatgatggaactaatgatgactatgcagactccgtgaagggccgattcaccatctccaaagacacctccaagaacactctctatctgcaaatgaacagcctgaggccgaggacacggctatatattattgtgcgagagcggctagccatgggtggtacctggactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [283, 284] c86>t,T29>I(- - -)|a106>g,S36>G(+ + -)|c134>t,A45>V(+ - +)|t135>c,A45>V(+ - +)|g147>c|a162>g|a168>t|t169>c,W57>R(- - -)|g188>c,S63>T(+ - +)|a193>g,K65>D(+ - -)|a195>t,K65>D(+ - -)|t196>g,Y66>D(- - -)|g239>a,R80>K(+ + +)|a245>c,N82>T(- + -)|g258>t|g261>c|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t|a317>g,K106>R(+ + +)| c86>t,T29>I(- - -)|a106>g,S36>G(+ + -)| c134>t,A45>V(+ - +)|t135>c,A45>V(+ - +)|g147>c|a162>g| a168>t|t169>c,W57>R(- - -)|g188>c,S63>T(+ - +)|a193>g,K65>D(+ - -)|a195>t,K65>D(+ - -)| t196>g,Y66>D(- - -)|g239>a,R80>K(+ + +)|a245>c,N82>T(- + -)|g258>t|g261>c|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)|c309>t| a317>g,K106>R(+ + +)|a320>c| 319 (320) 223 (224) 203 20 (21) 6 (7) 14 4 3 3 2 1 (2) 0 2 0 3 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 0 1 0 0 0 0 0 0 0 0 0 51 51 47 4 2 2 1 0 1 1 0 0 0 0 1 0 0 0 30 24 19 5 1 4 1 0 0 1 0 0 2 0 1 0 0 0 117 113 105 8 3 5 0 3 1 0 1 0 0 0 1 0 1 1 7 (8) 7 6 1 (2) 0 (1) 1 1 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|aact,244-247(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcatcttcagtggctatggc atgcactgggtccgccaggtcccaggcaagggcctggagtgggtggcggtt attcggtatgatggaactaatgat gactatgcagactccgtgaagggccgattcaccatctccaaagacacctccaagaacactctctatctgcaaatgaacagcctgaggccgaggacacggctatatattattgt gcgagagcggctagccatgggtggtacctggactcc ARAASHGWYLDS IGHV3-33 IGHD6-19 IGHJ4 ggattcatcttcagtggctatggc atgcactgggtccgccaggtcccaggcaagggcctggagtgggtggcggtt attcggtatgatggaactaatgat gactatgcagactccgtgaagggccgattcaccatctccaaagacacctccaagaacactctctatctgcaaatgaacagcctgaggccgaggacacggctatatattattgt gcgagagcggctagccatgggtggtacctggactcc unmatched 19 212 11 8 6 10 5 9 5 1 7 6 JY8QFUQ01ANU9C 17555 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 1004 95.87 209/218 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F 102 66.67 34/51 nt Homsap IGHD4-17*01 F 2 8 8 9 8.8.9 [1.17.38.11] CARDNYGDYWG in-frame + cctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtgttaatggtgatgggggtagcacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgtgcaagagataattacggtgactactgggggcccagggaacccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [276, 277] a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a187>g,S63>G(+ + -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| 320 226 217 9 4 5 4 1 0 0 0 0 0 0 3 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 3 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 4 2 1 1 0 0 0 0 0 0 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactactgg ARDNYGDYW IGHV3-74 IGHD4-17 IGHJ5 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatgggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactactgg unmatched 9 213 5 4 1 5 4 4 4 0 2 3 JY8QFUQ01AP4OP 13603 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 936 92.63 201/217 nt NA Homsap IGHJ6*02 F 256 90.32 56/62 nt Homsap IGHD1-26*01 F 1 8 8 18 8.8.18 [1.17.38.11] CAKVLAGATRDAYHYGMDVW in-frame + ctctggatttactttcagtaggtctgccatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggcattagtggtggtggtcttagcacatactacacagactccgtgaagggccggttcaccatctccagagacatttccaagaacacgctgtttctgcaaatgaacagcctgagagccgaggacacggccacatattactgtgcgaaagtcctggcgggagctacgagggacgcctaccactacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacacgcaccccccaagcatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 64 79 [302, 304] c84>t|c87>t|t90>c|c105>t|c108>g,S36>R(- - -)|a110>c,Y37>S(+ - -)|c164>g,A55>G(- + -)|t165>c,A55>G(- + -)|a175>g,S59>G(+ + -)|g187>c,G63>L(- - -)|g188>t,G63>L(- - -)|g202>a,A68>T(- - -)|a245>t,N82>I(- - -)|a263>t,Y88>F(- + -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)| c84>t|c87>t|t90>c|c105>t|c108>g,S36>R(- - -)|a110>c,Y37>S(+ - -)| c164>g,A55>G(- + -)|t165>c,A55>G(- + -)| a175>g,S59>G(+ + -)|g187>c,G63>L(- - -)|g188>t,G63>L(- - -)| g202>a,A68>T(- - -)|a245>t,N82>I(- - -)|a263>t,Y88>F(- + -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)| a320>t| 319 (320) 224 (225) 208 16 (17) 4 (5) 12 1 2 3 3 1 0 2 (3) 0 1 2 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 4 2 0 0 3 1 1 0 0 0 0 1 0 0 51 51 49 2 0 2 0 0 0 1 0 0 0 0 0 1 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 1 0 1 0 117 114 109 5 0 5 0 2 0 1 0 0 2 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatttactttcagtaggtctgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggc attagtggtggtggtcttagcaca tactacacagactccgtgaagggccggttcaccatctccagagacatttccaagaacacgctgtttctgcaaatgaacagcctgagagccgaggacacggccacatattactgt gcgaaagtcctggcgggagctacgagggacgcctaccactacggtatggacgtc AKVLAGATRDAYHYGMDV IGHV3-23 IGHD1-26 IGHJ6 ggatttactttcagtaggtctgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggc attagtggtggtggtcttagcaca tactacacagactccgtgaagggccggttcaccatctccagagacatttccaagaacacgctgtttctgcaaatgaacagcctgagagccgaggacacggccacatattactgt gcgaaagtcctggcgggagctacgagggacgcctaccactacggtatggacgtc unmatched 16 213 9 7 5 9 4 7 0 4 7 5 JY8QFUQ01APLX8 11642 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 855 88.48 192/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-3*01 F 3 8 8 10 8.8.10 [1.17.38.11] CASFRVLESGYW in-frame + ctctggattcacattcggtagttttatgatgaactgggtccgccaggctccagggaagggactggagtgggtcgcatcgattagccctactagtactttcatagactacgcagactcagtgaggggccggttcaccatctccagagataacgccgagaacttactgtatctgcaaatgaacggcctgagagtcgaagacacggctgtctattactgtgcgagttttcgtgtcctggaaagtggctactggggccagggaaccttggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [278, 279] c87>a|a103>g,S35>G(+ + -)|c108>t|a110>t,Y37>F(- + -)|g113>t,S38>M(- - -)|c114>g,S38>M(- - -)|g147>a|t160>g,S54>A(- + -)|c165>g|t171>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g176>c,S59>T(+ - +)|g188>c,S63>T(+ - +)|a191>t,Y64>F(- + -)|t196>g,Y66>D(- - -)|a215>g,K72>R(+ + +)|a225>g|c243>t|a250>g,K84>E(+ - -)|c257>t,S86>L(- - -)|a277>g,S93>G(+ + -)|c287>t,A96>V(+ - +)|g291>a|g303>c| c87>a|a103>g,S35>G(+ + -)|c108>t|a110>t,Y37>F(- + -)|g113>t,S38>M(- - -)|c114>g,S38>M(- - -)| g147>a|t160>g,S54>A(- + -)|c165>g| t171>c|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g176>c,S59>T(+ - +)|g188>c,S63>T(+ - +)|a191>t,Y64>F(- + -)| t196>g,Y66>D(- - -)|a215>g,K72>R(+ + +)|a225>g|c243>t|a250>g,K84>E(+ - -)|c257>t,S86>L(- - -)|a277>g,S93>G(+ + -)|c287>t,A96>V(+ - +)|g291>a|g303>c| a318>t,R106>S(- - -)|g319>t|a320>t| 317 (320) 222 (225) 197 25 (28) 9 (11) 16 (17) 5 2 4 1 1 1 2 (4) 0 4 2 1 (2) 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 2 4 1 0 1 0 0 1 1 0 0 1 1 0 51 51 48 3 2 1 0 1 0 0 0 0 0 0 0 1 0 1 30 24 18 6 1 5 0 0 0 1 1 0 1 0 3 0 0 0 117 114 104 10 4 6 4 1 3 0 0 0 0 0 1 0 0 1 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 0 0 0 0 0 0 (2) 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacattcggtagttttatg atgaactgggtccgccaggctccagggaagggactggagtgggtcgcatcg attagccctactagtactttcata gactacgcagactcagtgaggggccggttcaccatctccagagataacgccgagaacttactgtatctgcaaatgaacggcctgagagtcgaagacacggctgtctattactgt gcgagttttcgtgtcctggaaagtggctac ASFRVLESGY IGHV3-21 IGHD3-3 IGHJ4 ggattcacattcggtagttttatg atgaactgggtccgccaggctccagggaagggactggagtgggtcgcatcg attagccctactagtactttcata gactacgcagactcagtgaggggccggttcaccatctccagagataacgccgagaacttactgtatctgcaaatgaacggcctgagagtcgaagacacggctgtctattactgt gcgagttttcgtgtcctggaaagtggctac unmatched 25 213 12 13 6 14 6 11 6 3 7 9 JY8QFUQ01APOB5 16335 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 885 90.65 194/214 nt NA Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD2-15*01 F 3 8 7 15 8.7.15 [1.17.38.11] CTRELRVAAARDGVDIW in-frame + ctctggattcaccgtcagtaccaagttcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcagtaatttatcctgatggtactacacactatggagcctccgtgaggggccggttcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgtacgagagaacttcgggtggctgctgcgcgtgatggtgttgacatctggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [290, 291] g81>a|g107>c,S36>T(+ - +)|c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)|t165>a|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|t196>c,Y66>H(+ - -)|c201>t|c203>g,A68>G(- + -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a225>g|t262>g,Y88>D(- - -)|g303>t|c309>t|g313>a,A105>T(- - -)| g81>a|g107>c,S36>T(+ - +)|c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)| t165>a| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)| t196>c,Y66>H(+ - -)|c201>t|c203>g,A68>G(- + -)|a206>c,D69>A(- - -)|a215>g,K72>R(+ + +)|a225>g|t262>g,Y88>D(- - -)|g303>t|c309>t| g313>a,A105>T(- - -)| 320 222 201 21 6 15 2 3 4 1 2 0 1 1 3 2 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 0 0 0 0 1 0 1 1 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 21 15 6 0 6 0 1 2 0 1 0 0 0 2 0 0 0 117 114 105 9 4 5 2 0 2 1 1 0 0 0 0 1 1 1 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccgtcagtaccaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagta atttatcctgatggtactaca cactatggagcctccgtgaggggccggttcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttcgggtggctgctgcgcgtgatggtgttgacatc TRELRVAAARDGVDI IGHV3-53 IGHD2-15 IGHJ3 ggattcaccgtcagtaccaagttc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagta atttatcctgatggtactaca cactatggagcctccgtgaggggccggttcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttcgggtggctgctgcgcgtgatggtgttgacatc unmatched 20 210 9 11 6 12 3 8 5 1 5 9 JY8QFUQ01APXHX 20484 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 954 93.55 203/217 nt 93.09 202/217 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD6-13*01 F 3 8 8 11 8.8.11 [1.17.38.11] CVRDRSGQAVGYW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 74 of V-REGION: 'G' (1 nucleotides at position 125 to 125 in the user submitted sequence), (cause frameshift); ctctggattcaccttcagtagttactggatgcactgggtccgccaagttccagggaagggactggtgtgggtctcacgaattaatactgatgggagtgccacaagttacgcggactccgtgaggGggccgattcaccatctccagagacaacgccaagaacacgctatatcttcaaatgaacagtctgagagtcgaagacacggctgtctattactgtgtaagagatcggtccggtcaggcggtaggatactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccggagagccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA unmatched, IGG2 64 88 [283] c108>t|c134>t,A45>V(+ - +)|g147>a|t165>a|g173>c,S58>T(+ - +)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|c198>t|a215>g,K72>R(+ + +)|g261>a|g267>t|c287>t,A96>V(+ - +)|g291>a|g303>c|c314>t,A105>V(+ - +)| c108>t| c134>t,A45>V(+ - +)|g147>a|t165>a| g173>c,S58>T(+ - +)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| c198>t|a215>g,K72>R(+ + +)|g261>a|g267>t|c287>t,A96>V(+ - +)|g291>a|g303>c| c314>t,A105>V(+ - +)| 320 225 210 15 8 7 2 3 5 0 0 0 0 1 3 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 1 0 0 0 0 30 24 21 3 0 3 1 0 0 0 0 0 0 0 2 0 0 0 117 114 107 7 5 2 1 2 2 0 0 0 0 0 1 0 1 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagttactgg atgcactgggtccgccaagttccagggaagggactggtgtgggtctcacga attaatactgatgggagtgccaca agttacgcggactccgtgaggggccgattcaccatctccagagacaacgccaagaacacgctatatcttcaaatgaacagtctgagagtcgaagacacggctgtctattactgt gtaagagatcggtccggtcaggcggtaggatac VRDRSGQAVGY IGHV3-74 IGHD6-13 IGHJ4 ggattcaccttcagtagttactgg atgcactgggtccgccaagttccagggaagggactggtgtgggtctcacga attaatactgatgggagtgccaca agttacgcggactccgtgaggggccgattcaccatctccagagacaacgccaagaacacgctatatcttcaaatgaacagtctgagagtcgaagacacggctgtctattactgt gtaagagatcggtccggtcaggcggtaggatac unmatched 14 213 9 5 7 11 2 3 7 1 3 3 JY8QFUQ01AQ36T 6218 productive Homsap IGHV3-7*01 F 968 94.04 205/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSGFYSTGYW in-frame + gctctggattcagctttagttattattggatgagctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaaggaagatggaagtgagagacactatgtggactctgtgatgggccggttcaccatctccagagacaacgccaagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgtgcgattagtagtggtttttactcgacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [282, 283] c74>g|c86>g,T29>S(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c108>t,S36>Y(+ - -)|c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)|t196>c,Y66>H(+ - -)|a215>t,K72>M(- + -)|a225>g|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)|g303>a| c74>g| c86>g,T29>S(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c108>t,S36>Y(+ - -)| c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)| t196>c,Y66>H(+ - -)|a215>t,K72>M(- + -)|a225>g|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)|g303>a| g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 316 (320) 222 (226) 209 13 (17) 3 (5) 10 (12) 3 (4) 2 (3) 1 1 0 0 2 (3) 0 0 4 0 (1) 0 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 20 4 0 4 0 1 1 0 0 0 1 0 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 1 0 0 117 114 108 6 2 4 2 1 0 1 0 0 1 0 0 1 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gctct ggattcagctttagttattattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagaga cactatgtggactctgtgatgggccggttcaccatctccagagacaacgccaagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgattagtagtggtttttactcgacgggctac AISSGFYSTGY IGHV3-7 IGHD3-22 IGHJ4 ggattcagctttagttattattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagaga cactatgtggactctgtgatgggccggttcaccatctccagagacaacgccaagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgattagtagtggtttttactcgacgggctac unmatched 12 213 7 5 3 6 4 6 2 0 4 6 JY8QFUQ01AQ6RF 17835 productive (see comment) Homsap IGHV3-30*01 F, or Homsap IGHV3-30*17 F 954 93.55 203/217 nt 92.63 201/217 nt Homsap IGHJ4*02 F (see comment) 177 85.42 41/48 nt Homsap IGHD2-21*01 F 2 8 8 14 8.8.14 [1.17.38.11] CATDRKYSGVRGDDSW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) in FR2-IMGT at codon 41 of V-REGION: 'G' (1 nucleotides at position 37 to 37 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 61 to 61 in the user submitted sequence), (cause frameshift); ctctggattcaccttcaggaactatgctatgcactgGggtccgccaggctcctggcaaggGggctagaatgggtggcttttatatcatatgatggaagtagtcaatactacgcagactccgtgaagggccgattcaccatctccagagacaactccaagaacacactttatctgcaaatgaacagcctgagaggtgacgacacggctgtgttttactgtgcgacggaccggaaatatagtggtgtacgaggcgatgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccgaagatgggaaccgtggtccgtcgcatgcctggtccagggcttcttcccc NA unmatched, IGA2 64 79 [292] t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|a138>t|g153>a|a162>t|g163>t,V55>F(+ - -)|a191>g,N64>S(- - -)|a193>c,K65>Q(+ - -)|t246>c|g258>a|g261>t|c287>g,A96>G(- + -)|g291>c,E97>D(+ - +)|a305>t,Y102>F(- + -)| t105>g,S35>R(- - -)|g107>a,S36>N(- - -)| a138>t|g153>a|a162>t|g163>t,V55>F(+ - -)| a191>g,N64>S(- - -)|a193>c,K65>Q(+ - -)| t246>c|g258>a|g261>t|c287>g,A96>G(- + -)|g291>c,E97>D(+ - +)|a305>t,Y102>F(- + -)| g317>c,R106>T(- - -)|a318>g,R106>T(- - -)| 316 (320) 221 (225) 207 (209) 14 (16) 6 8 (10) 1 (2) 3 0 1 1 0 3 0 1 (2) 1 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 51 51 47 4 3 1 0 1 0 0 0 0 2 0 0 0 1 0 30 24 22 2 0 2 1 0 0 0 1 0 0 0 0 0 0 0 117 114 108 6 3 3 0 1 0 1 0 0 1 0 1 1 1 0 4 (8) 4 4 (6) 0 (2) 0 0 (2) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcaggaactatgct atgcactgggtccgccaggctcctggcaaggggctagaatgggtggctttt atatcatatgatggaagtagtcaa tactacgcagactccgtgaagggccgattcaccatctccagagacaactccaagaacacactttatctgcaaatgaacagcctgagaggtgacgacacggctgtgttttactgt gcgacggaccggaaatatagtggtgtacgaggcgatgactcc ATDRKYSGVRGDDS IGHV3-30 IGHD2-21 IGHJ4 ggattcaccttcaggaactatgct atgcactgggtccgccaggctcctggcaaggggctagaatgggtggctttt atatcatatgatggaagtagtcaa tactacgcagactccgtgaagggccgattcaccatctccagagacaactccaagaacacactttatctgcaaatgaacagcctgagaggtgacgacacggctgtgttttactgt gcgacggaccggaaatatagtggtgtacgaggcgatgactcc unmatched 14 213 5 9 3 7 2 7 6 0 4 4 JY8QFUQ01AQQ9J 3119 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 945 93.09 202/217 nt NA Homsap IGHJ3*02 F 232 96 48/50 nt Homsap IGHD3-22*01 F 2 8 8 19 8.8.19 [1.17.38.11] CAKDREDSSGYYFFRDGFDIW in-frame + ctctggattcacgtttaggacctatgccatgacctgggtccgccaggctccagggaaggggctggagtgggtctccggtattggtggtagtggtcgaaccacacactacgcagactccgtgcagggccggttcaccatctccagagacaattccaagaacacggtggatctgcaaatgaacagcctgagagccgaggacacggccatatattactgtgcgaaagatcgtgaagatagtagtggttattacttcttccgtgatggttttgatatttggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [305, 306] c87>g|c105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|g119>c,S40>T(+ - +)|a162>c|c164>g,A55>G(- + -)|a169>g,S57>G(+ + -)|g187>c,G63>R(- - -)|t189>a,G63>R(- - -)|g191>c,S64>T(+ - +)|t196>c,Y66>H(+ - -)|a214>c,K72>Q(+ - -)|c259>g,L87>V(+ - +)|t262>g,Y88>D(- - -)|g301>a,V101>I(+ - +)| c87>g|c105>g,S35>R(- - -)|g107>c,S36>T(+ - +)| g119>c,S40>T(+ - +)|a162>c|c164>g,A55>G(- + -)| a169>g,S57>G(+ + -)|g187>c,G63>R(- - -)|t189>a,G63>R(- - -)|g191>c,S64>T(+ - +)| t196>c,Y66>H(+ - -)|a214>c,K72>Q(+ - -)|c259>g,L87>V(+ - +)|t262>g,Y88>D(- - -)|g301>a,V101>I(+ - +)| 320 225 210 15 2 13 1 1 0 1 2 0 0 1 4 4 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 0 0 0 0 0 0 1 2 0 0 51 51 48 3 1 2 0 0 0 0 1 0 0 0 1 1 0 0 30 24 20 4 0 4 1 0 0 0 0 0 0 1 2 0 0 0 117 114 109 5 0 5 0 1 0 1 1 0 0 0 0 1 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgtttaggacctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctccggt attggtggtagtggtcgaaccaca cactacgcagactccgtgcagggccggttcaccatctccagagacaattccaagaacacggtggatctgcaaatgaacagcctgagagccgaggacacggccatatattactgt gcgaaagatcgtgaagatagtagtggttattacttcttccgtgatggttttgatatt AKDREDSSGYYFFRDGFDI IGHV3-23 IGHD3-22 IGHJ3 ggattcacgtttaggacctatgcc atgacctgggtccgccaggctccagggaaggggctggagtgggtctccggt attggtggtagtggtcgaaccaca cactacgcagactccgtgcagggccggttcaccatctccagagacaattccaagaacacggtggatctgcaaatgaacagcctgagagccgaggacacggccatatattactgt gcgaaagatcgtgaagatagtagtggttattacttcttccgtgatggttttgatatt unmatched 15 213 3 12 1 9 2 6 1 1 7 6 JY8QFUQ01AR5SV 5451 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 3 8 8 13 8.8.13 [1.17.38.11] CARGLAVILGGFDYW in-frame + ctctggattcaccttcagtagttatagcatgcactgggtccgccaggctccagggaagggactggagtgggtctcctccattagtagtaatagtgcctacacagaccacgcagactcagtgaagggccgattcaccatctccagagacaacgacaagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagggctagcagtgattcttggaggatttgactactggggccagggaaacctggtcaccgtctcctcagcctccacaagggcccatcggtcttcccctggcacctcctccaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA unmatched, IGG1 64 69 [285, 287] c108>t|a118>c,N40>H(- - -)|g147>a|a162>c|g176>a,S59>N(- - -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>c,S63>A(- + -)|t194>c,I65>T(- - -)|t196>g,Y66>D(- - -)|t199>c,Y67>H(+ - -)|c248>a,A83>D(- - -)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)| c108>t| a118>c,N40>H(- - -)|g147>a|a162>c| g176>a,S59>N(- - -)|a187>g,S63>A(- + -)|g188>c,S63>A(- + -)|t189>c,S63>A(- + -)|t194>c,I65>T(- - -)| t196>g,Y66>D(- - -)|t199>c,Y67>H(+ - -)|c248>a,A83>D(- - -)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)| a320>g| 319 (320) 224 (225) 210 14 (15) 3 (4) 11 2 (3) 2 1 3 2 1 0 0 1 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 0 0 2 0 0 0 0 0 0 0 30 24 19 5 0 5 1 1 0 2 0 0 0 0 1 0 0 0 117 114 109 5 0 5 1 0 0 1 0 1 0 0 0 1 0 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagttatagc atgcactgggtccgccaggctccagggaagggactggagtgggtctcctcc attagtagtaatagtgcctacaca gaccacgcagactcagtgaagggccgattcaccatctccagagacaacgacaagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggctagcagtgattcttggaggatttgactac ARGLAVILGGFDY IGHV3-21 IGHD3-22 IGHJ4 ggattcaccttcagtagttatagc atgcactgggtccgccaggctccagggaagggactggagtgggtctcctcc attagtagtaatagtgcctacaca gaccacgcagactcagtgaagggccgattcaccatctccagagacaacgacaagaggtcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagggctagcagtgattcttggaggatttgactac unmatched 14 213 8 6 3 6 5 8 2 1 6 5 JY8QFUQ01ARLVF 10888 productive Homsap IGHV3-7*01 F 837 87.56 190/217 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 147 76.47 39/51 nt Homsap IGHD5-12*01 F 3 8 8 13 8.8.13 [1.17.38.11] CGRDERGADLPNDSW in-frame + Other possibilities: Homsap_IGHJ5*02 and Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctggattcacctttagtaggtattggatgagctgggtccgccagtctccagggaagggactggagtggctggcccacataggaggagatggaagtgaggctggttatgtggactctgtgaggggccgattcttcatctccagagacaacgccaagagctccctctatctgcagatgaacagcctgagccccgaggacacgggtgtgtattattgtggcagagatgaaaggggcgccgacttgccgaatgattcctggggcctgggaaccctggtcaccgtctcctcaccatcccctgacccagccccaaggtcttccgctgcagctcgacagcaccccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 86 63 [288] c108>g,S36>R(- - -)|g133>t,A45>S(- + -)|g147>a|g157>c,V53>L(+ - +)|a163>c,N55>H(- - -)|a169>g,K57>G(- - -)|a170>g,K57>G(- - -)|g171>a,K57>G(- - -)|c172>g,Q58>G(- - -)|a173>g,Q58>G(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)|a195>t,K65>A(- - -)|t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>t,Y66>G(+ - -)|a215>g,K72>R(+ + +)|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|a254>g,N85>S(- - -)|a258>c|g261>c|a270>g|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|c299>g,A100>G(- + -)|c309>t|c314>g,A105>G(- + -)|g315>c,A105>G(- + -)| c108>g,S36>R(- - -)| g133>t,A45>S(- + -)|g147>a|g157>c,V53>L(+ - +)|a163>c,N55>H(- - -)| a169>g,K57>G(- - -)|a170>g,K57>G(- - -)|g171>a,K57>G(- - -)|c172>g,Q58>G(- - -)|a173>g,Q58>G(- - -)|a193>g,K65>A(- - -)|a194>c,K65>A(- - -)|a195>t,K65>A(- - -)| t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>t,Y66>G(+ - -)|a215>g,K72>R(+ + +)|a229>t,T77>F(- - -)|c230>t,T77>F(- - -)|a254>g,N85>S(- - -)|a258>c|g261>c|a270>g|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|c299>g,A100>G(- + -)|c309>t| c314>g,A105>G(- + -)|g315>c,A105>G(- + -)| 320 225 196 29 5 24 8 2 3 0 4 0 2 0 4 4 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 47 4 1 3 0 1 0 0 1 0 0 0 1 0 1 0 30 24 16 8 0 8 4 1 0 0 1 0 1 0 0 1 0 0 117 114 100 14 4 10 4 0 3 0 2 0 1 0 2 1 0 1 8 8 6 2 0 2 0 0 0 0 0 0 0 0 1 1 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtaggtattgg atgagctgggtccgccagtctccagggaagggactggagtggctggcccac ataggaggagatggaagtgaggct ggttatgtggactctgtgaggggccgattcttcatctccagagacaacgccaagagctccctctatctgcagatgaacagcctgagccccgaggacacgggtgtgtattattgt ggcagagatgaaaggggcgccgacttgccgaatgattcc GRDERGADLPNDS IGHV3-7 IGHD5-12 IGHJ5 ggattcacctttagtaggtattgg atgagctgggtccgccagtctccagggaagggactggagtggctggcccac ataggaggagatggaagtgaggct ggttatgtggactctgtgaggggccgattcttcatctccagagacaacgccaagagctccctctatctgcagatgaacagcctgagccccgaggacacgggtgtgtattattgt ggcagagatgaaaggggcgccgacttgccgaatgattcc unmatched 27 213 13 14 5 12 8 15 5 0 13 9 JY8QFUQ01ARS0N 16033 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 896 90.37 197/218 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-9*01 F 2 8 8 10 8.8.10 [1.17.38.11] CVSDLAGQKGFW in-frame + cctcaggattcaccttcagtaggtactggatgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgtattaatgaagacggcagctacacagatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtttttgcaaatgaacagtctgagaggcgaggacacggctgtctattactgtgttagcgatttggctggtcagaaaggtttctggggccagggagtcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [279, 280] t78>a|c108>g,S36>R(- - -)|c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|a263>t,Y88>F(- + -)|c265>t|c287>g,A96>G(- + -)|g303>c| t78>a| c108>g,S36>R(- - -)| c134>t,A45>V(+ - +)|t149>c,L50>P(- - -)| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t177>c|g186>c|t189>c|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c198>t,S66>D(- - -)|t199>c,Y67>H(+ - -)|c257>a,T86>K(- - -)|a263>t,Y88>F(- + -)|c265>t|c287>g,A96>G(- + -)|g303>c| c314>t,A105>V(+ - +)|a315>t,A105>V(+ - +)|a318>c,R106>S(- - -)| 320 226 202 24 6 18 2 3 4 4 1 1 3 2 2 2 0 0 78 5 4 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 49 2 0 2 0 0 1 1 0 0 0 0 0 0 0 0 30 24 16 8 3 5 1 2 0 2 0 0 1 1 1 0 0 0 117 114 105 9 2 7 1 1 2 1 0 1 1 0 1 1 0 0 8 8 5 3 0 3 0 0 1 0 1 0 1 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctca ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagctacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtttttgcaaatgaacagtctgagaggcgaggacacggctgtctattactgt gttagcgatttggctggtcagaaaggtttc VSDLAGQKGF IGHV3-74 IGHD3-9 IGHJ4 ggattcaccttcagtaggtactgg atgcactgggtccgccaagttccagggaaggggccggtgtgggtctcacgt attaatgaagacggcagctacaca gatcacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtttttgcaaatgaacagtctgagaggcgaggacacggctgtctattactgt gttagcgatttggctggtcagaaaggtttc unmatched 20 213 12 8 6 11 6 9 2 3 9 6 JY8QFUQ01AT43H 1611 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 872 89.77 193/215 nt 89.3 192/215 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-10*01 F 3 8 8 10 8.8.10 [1.16.38.11] CSKDTFGREDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT, from codon 48 of V-REGION: 2 nucleotides (from position 57 in the user submitted sequence), (cause frameshift) ctctggattctccgtcagtaattactggatgcactgggtccgccaggctccagggagggctggagtgggtctcacgtattaatgaagatggtagtcggacagactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgtagtaaagacacatttgggcgagaagactactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaacgatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 84 [276, 278] a85>t,T29>S(+ - +)|t88>g,F30>V(+ - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a132>g|t152>a,V51>E(- + -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>t|a190>c,S64>R(- - -)|c192>g,S64>R(- - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c234>t|c236>a,S79>Y(+ - -)|a245>g,N82>S(- - -)|g258>a|c287>t,A96>V(+ - +)|g291>a|g303>c|c309>t| a85>t,T29>S(+ - +)|t88>g,F30>V(+ - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a132>g|t152>a,V51>E(- + -)| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>t|a190>c,S64>R(- - -)|c192>g,S64>R(- - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c234>t|c236>a,S79>Y(+ - -)|a245>g,N82>S(- - -)|g258>a|c287>t,A96>V(+ - +)|g291>a|g303>c|c309>t| g313>a,A105>S(- + -)|c314>g,A105>S(- + -)|a315>t,A105>S(- + -)|g317>a,R106>K(+ + +)| 320 223 197 26 7 19 4 7 4 0 1 1 2 2 1 2 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 1 0 0 0 1 0 0 0 0 1 51 49 47 2 1 1 1 0 0 0 0 0 0 1 0 0 0 0 30 24 18 6 1 5 1 1 0 0 1 0 0 1 0 1 1 0 117 114 104 10 5 5 2 3 3 0 0 1 0 0 1 0 0 0 8 8 4 4 0 4 0 2 0 0 0 0 1 0 0 1 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattctccgtcagtaattactgg atgcactgggtccgccaggctccagggagggctggagtgggtctcacgt attaatgaagatggtagtcggaca gactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgt agtaaagacacatttgggcgagaagactac SKDTFGREDY IGHV3-74 IGHD3-10 IGHJ4 ggattctccgtcagtaattactgg atgcactgggtccgccaggctccagggagggctggagtgggtctcacgt attaatgaagatggtagtcggaca gactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgt agtaaagacacatttgggcgagaagactac unmatched 22 211 13 9 9 13 4 9 6 1 6 9 JY8QFUQ01AT8N2 21858 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 882 89.86 195/217 nt 88.94 193/217 nt Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [1.17.38.11] CTRDIGGRYSYW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'T' (1 nucleotides at position 16 to 16 in the user submitted sequence), (cause frameshift);in FR2-IMGT at codon 47 of V-REGION: 'G' (1 nucleotides at position 55 to 55 in the user submitted sequence), (cause frameshift); ctccggattcaccttTtagtagtttctggatgcactgggtccgccaagttccagGgggagggactggtgtgggtcgcacggactaatgagtatgggagtatcacaaactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgtacaagggacatcggtgggcgctactcctactggggccagggagtcctggtctccgtctcatcagcatcccccgaccagccccaaggtcttccccgctgagccctcgacagcaccccccccaaagatgggaacgtggtcgtcgcatgcctggtcccaggggcttcttcccccca NA unmatched, IGA2 57 79 [286, 281, 283] t78>c|c90>t|c108>t|a110>t,Y37>F(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g247>a,A83>T(- - -)|c257>g,T86>R(- - -)|g261>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|g313>a,A105>T(- - -)|a318>g| t78>c| c90>t|c108>t|a110>t,Y37>F(- + -)| c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g147>a|t160>g,S54>A(- + -)|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>g,S58>E(- - -)|g175>t,D59>Y(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g247>a,A83>T(- - -)|c257>g,T86>R(- - -)|g261>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| g313>a,A105>T(- - -)|a318>g| 320 225 201 24 7 17 4 7 3 2 0 0 1 0 0 1 3 3 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 2 0 0 0 1 0 0 0 0 0 51 51 46 5 2 3 1 1 1 0 0 0 0 0 0 0 0 2 30 24 18 6 0 6 1 1 0 1 0 0 0 0 0 0 2 1 117 114 107 7 1 6 1 4 0 0 0 0 0 0 0 1 1 0 8 8 6 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcacctttagtagtttctgg atgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgt acaagggacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttagtagtttctgg atgcactgggtccgccaagttccaggggagggactggtgtgggtcgcacgg actaatgagtatgggagtatcaca aactacgcggactccgtggagggccgattcaccatctccagagacaacaccaagaacaggctatatctgcaaatgaacagtctgagagccgaggacacggctatttattactgt acaagggacatcggtgggcgctactcctac unmatched 21 213 13 8 9 13 4 8 3 2 9 7 JY8QFUQ01ATKPT 12689 productive Homsap IGHV3-48*01 F 864 88.94 193/217 nt NA Homsap IGHJ3*02 F 223 94 47/50 nt Homsap IGHD3-22*01 F 2 8 8 19 8.8.19 [1.17.38.11] CARASTFYYDGSGYRDVFDIW in-frame + ctctggcttcagtttgagtacttataccatgaactgggtccgccaggctccagggaaggggctggagtgggtttcactcattagtaagactagtaatgtcatatactacgcggactctgtgaagggccggttcaccatctccagagacaatgccgagaattcactgtttctgcaaatggacagcctgagtgccgaggacacgggtgtatattactgtgcgagagcttcgacgttctactacgatggcagtggttatcgtgatgtttttgatatatggggccaagggacaatggtcaccgtctcctcagcctccaccaagggccctatcggtcttccccgtggcaccctcctccaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG1 79 69 [307] a81>c|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g113>c,S38>T(+ - +)|t163>c,Y55>L(- - -)|a164>t,Y55>L(- - -)|g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g176>c,S59>T(+ - +)|g188>a,S63>N(- - -)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)|a204>g|a225>g|a250>g,K84>E(+ - -)|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|a285>t,R95>S(- - -)|c299>g,A100>G(- + -)|g303>a| a81>c|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>g,F30>L(+ - -)|g107>c,S36>T(+ - +)|c108>t,S36>T(+ - +)|g113>c,S38>T(+ - +)| t163>c,Y55>L(- - -)|a164>t,Y55>L(- - -)| g173>a,S58>K(- - -)|t174>g,S58>K(- - -)|g176>c,S59>T(+ - +)|g188>a,S63>N(- - -)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)| a204>g|a225>g|a250>g,K84>E(+ - -)|c255>t|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|a285>t,R95>S(- - -)|c299>g,A100>G(- + -)|g303>a| a320>c| 319 (320) 224 (225) 200 24 (25) 5 (6) 19 5 3 4 1 1 (2) 0 3 0 3 3 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 1 6 0 0 2 0 1 0 0 0 2 2 0 0 51 51 49 2 0 2 0 0 0 1 0 0 1 0 0 0 0 0 30 24 18 6 0 6 1 2 1 0 0 0 0 0 1 0 0 1 117 114 105 9 4 5 4 1 1 0 0 0 2 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggcttcagtttgagtacttatacc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcactc attagtaagactagtaatgtcata tactacgcggactctgtgaagggccggttcaccatctccagagacaatgccgagaattcactgtttctgcaaatggacagcctgagtgccgaggacacgggtgtatattactgt gcgagagcttcgacgttctactacgatggcagtggttatcgtgatgtttttgatata ARASTFYYDGSGYRDVFDI IGHV3-48 IGHD3-22 IGHJ3 ggcttcagtttgagtacttatacc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcactc attagtaagactagtaatgtcata tactacgcggactctgtgaagggccggttcaccatctccagagacaatgccgagaattcactgtttctgcaaatggacagcctgagtgccgaggacacgggtgtatattactgt gcgagagcttcgacgttctactacgatggcagtggttatcgtgatgtttttgatata unmatched 24 213 13 11 7 13 6 11 4 1 7 12 JY8QFUQ01AU2BA 14154 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 963 94.01 204/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 2 8 8 15 8.8.15 [1.17.38.11] CARDMGSGRYFEPYDYW in-frame + gtctggattcatcttcttgaaatatggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaggtatatggtttgatggaagtaatacatactatgcggactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatttgcaactgaacagcctgagagccgaggacacggctgtgtattactgtgcgagagatatgggttcgggaagatattttgaaccttatgactactggggccagggaaccccggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG1 64 88 [294] c86>t,T29>I(- - -)|a103>t,S35>L(- - -)|g104>t,S35>L(- - -)|t105>g,S35>L(- - -)|g107>a,S36>K(- - -)|c108>a,S36>K(- - -)|t164>g,V55>G(- - -)|a173>t,Y58>F(- + -)|a194>c,K65>T(- - -)|a204>g|c259>g,L87>V(+ - +)|c265>t|a271>c,M91>L(+ + -)| c86>t,T29>I(- - -)|a103>t,S35>L(- - -)|g104>t,S35>L(- - -)|t105>g,S35>L(- - -)|g107>a,S36>K(- - -)|c108>a,S36>K(- - -)| t164>g,V55>G(- - -)| a173>t,Y58>F(- + -)|a194>c,K65>T(- - -)| a204>g|c259>g,L87>V(+ - +)|c265>t|a271>c,M91>L(+ + -)| 320 225 212 13 2 11 1 1 2 0 2 1 2 0 0 1 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 1 1 0 0 1 1 0 0 0 1 1 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 30 24 22 2 0 2 0 0 0 0 1 0 1 0 0 0 0 0 117 114 110 4 2 2 1 0 1 0 1 0 0 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcatcttcttgaaatatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaggt atatggtttgatggaagtaataca tactatgcggactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatttgcaactgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagatatgggttcgggaagatattttgaaccttatgactac ARDMGSGRYFEPYDY IGHV3-33 IGHD3-10 IGHJ4 ggattcatcttcttgaaatatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaggt atatggtttgatggaagtaataca tactatgcggactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtgtatttgcaactgaacagcctgagagccgaggacacggctgtgtattactgt gcgagagatatgggttcgggaagatattttgaaccttatgactac unmatched 13 213 4 9 3 6 1 7 2 0 3 8 JY8QFUQ01AUBMD 9861 productive Homsap IGHV1-18*01 F 948 93.93 201/214 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt NA 8 8 8 8.8.8 [0.17.38.11] CARGDYFDHW in-frame + tggttacacctttaccagctatggtctcagctgggtgcgacaggcccctggccaagggcttgagtggatgggatggatcttcgtttttaacggtaacacaaaatatgcacagcacctccagggcagagtcaccatgaccacagacacatccacggacacagcctacatggagctgaggagcctgagatctgacgacacggccgtgtattactgtgcgagaggtgactactttgaccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagcagatgggaacgtggtcatccgcctgcctggtccagggcttcttccccca NA unmatched, IGA1 50 84 [269, 268] a115>c,I39>L(+ + +)|a141>c|a169>t,S57>F(- - -)|g170>t,S57>F(- - -)|c173>t,A58>V(+ - +)|a176>t,Y59>F(- + -)|c177>t,Y59>F(- + -)|t186>c|c198>a,N66>K(+ - -)|a208>c,K70>H(- - +)|g210>c,K70>H(- - +)|a253>g,S85>D(- - -)|g254>a,S85>D(- - -)| a115>c,I39>L(+ + +)|a141>c| a169>t,S57>F(- - -)|g170>t,S57>F(- - -)|c173>t,A58>V(+ - +)|a176>t,Y59>F(- + -)|c177>t,Y59>F(- + -)|t186>c| c198>a,N66>K(+ - -)|a208>c,K70>H(- - +)|g210>c,K70>H(- - +)|a253>g,S85>D(- - -)|g254>a,S85>D(- - -)| a320>g| 319 (320) 221 (222) 208 13 (14) 2 (3) 11 1 (2) 1 2 1 3 1 2 0 1 0 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 0 0 0 2 0 0 0 0 0 0 0 30 24 18 6 1 5 0 0 2 1 0 0 2 0 0 0 1 0 117 114 109 5 0 5 1 1 0 0 1 1 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|ta,175-176(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,5-6(FR1)|tt,75-76(FR1)|tt,81-82(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|tt,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,3-6(FR1)|agct,8-11(FR1)|agct,24-27(FR1)|ggct,72-75(FR1)|ggtt,79-82(CDR1)|agct,106-109(CDR1)|ggta,112-115(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)| agct,8-11(FR1)|agct,24-27(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|agct,209-212(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggttacacctttaccagctatggt ctcagctgggtgcgacaggcccctggccaagggcttgagtggatgggatgg atcttcgtttttaacggtaacaca aaatatgcacagcacctccagggcagagtcaccatgaccacagacacatccacggacacagcctacatggagctgaggagcctgagatctgacgacacggccgtgtattactgt gcgagaggtgactactttgaccac ARGDYFDH IGHV1-18 IGHJ4 ggttacacctttaccagctatggt ctcagctgggtgcgacaggcccctggccaagggcttgagtggatgggatgg atcttcgtttttaacggtaacaca aaatatgcacagcacctccagggcagagtcaccatgaccacagacacatccacggacacagcctacatggagctgaggagcctgagatctgacgacacggccgtgtattactgt gcgagaggtgactactttgaccac unmatched 13 213 5 8 3 6 2 7 1 1 6 5 JY8QFUQ01AVBV0 8229 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 858 89.25 191/214 nt 88.32 189/214 nt Homsap IGHJ3*02 F 232 96 48/50 nt Homsap IGHD2-15*01 F 2 8 7 20 8.7.20 [1.16.38.11] CARDPRDYCSGGYCFVGAFDIW in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 77 of V-REGION: 'C' (1 nucleotides at position 132 to 132 in the user submitted sequence), (cause frameshift); in FR2-IMGT, from codon 47 of V-REGION: 1 nucleotides (from position 56 in the user submitted sequence), (cause frameshift) tctctggtggctccatgaggaattattactggagctggatccggcagtccccaggaagggactggagttgatagggactgtctattacactgggcgcacggagtacaacccctccctcaagagtcgactcaCccttatcactagacacgtccaagaaccagttctccctaaagctgggctctgtgaccgctgcggactcggccatttattactgtgcgagagacccccgagactattgtagtggtggttactgttttgttggtgcttttgatatctggggccaagggacaaaggtcaccgtctcttcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG1 57 100 [307] c90>g,I30>M(+ + -)|t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t|c133>t,P45>S(+ - -)|g155>t,W52>L(+ - -)|t159>a|t163>a,Y55>T(+ - -)|a164>c,Y55>T(+ - -)|a166>g,I56>V(+ - +)|g176>c,S59>T(+ - +)|a190>c,S64>R(- - -)|c195>g|a196>g,N66>E(+ - -)|c198>g,N66>E(+ - -)|g226>c,V76>L(+ - +)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|g267>a|a274>g,S92>G(+ + -)|a295>t,T99>S(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| c90>g,I30>M(+ + -)|t105>g,S35>R(- - -)|g107>a,S36>N(- - -)|c111>t| c133>t,P45>S(+ - -)|g155>t,W52>L(+ - -)|t159>a|t163>a,Y55>T(+ - -)|a164>c,Y55>T(+ - -)| a166>g,I56>V(+ - +)|g176>c,S59>T(+ - +)|a190>c,S64>R(- - -)|c195>g| a196>g,N66>E(+ - -)|c198>g,N66>E(+ - -)|g226>c,V76>L(+ - +)|a232>t,I78>L(+ + +)|g238>c,V80>L(+ - +)|g267>a|a274>g,S92>G(+ + -)|a295>t,T99>S(+ - +)|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| 320 222 199 23 4 19 3 3 2 0 2 0 2 2 3 3 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 1 0 0 0 0 0 0 1 0 1 51 50 45 5 1 4 0 0 1 0 1 0 0 2 0 0 1 0 30 21 17 4 1 3 1 0 0 0 1 0 0 0 1 1 0 0 117 114 104 10 1 9 2 2 0 0 0 0 2 0 2 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatgaggaattattac tggagctggatccggcagtccccaggaagggactggagttgatagggact gtctattacactgggcgcacg gagtacaacccctccctcaagagtcgactcaccttatcactagacacgtccaagaaccagttctccctaaagctgggctctgtgaccgctgcggactcggccatttattactgt gcgagagacccccgagactattgtagtggtggttactgttttgttggtgcttttgatatc ARDPRDYCSGGYCFVGAFDI IGHV4-59 IGHD2-15 IGHJ3 ggtggctccatgaggaattattac tggagctggatccggcagtccccaggaagggactggagttgatagggact gtctattacactgggcgcacg gagtacaacccctccctcaagagtcgactcaccttatcactagacacgtccaagaaccagttctccctaaagctgggctctgtgaccgctgcggactcggccatttattactgt gcgagagacccccgagactattgtagtggtggttactgttttgttggtgcttttgatatc unmatched 23 209 8 15 5 13 3 10 2 2 13 6 JY8QFUQ01AVKTX 17138 productive (see comment) Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 872 89.77 193/215 nt 89.3 192/215 nt Homsap IGHJ4*02 F 123 72.92 35/48 nt Homsap IGHD6-19*01 F 1 8 8 10 8.8.10 [0.17.38.11] CMNELGYCSGGG in-frame - Potentially, because of detected insertion/deletion in FR3-IMGT at codon 74 of V-REGION: 'G' (1 nucleotides at position 123 to 123 in the user submitted sequence), (cause frameshift); ctggattcacttttagcagccatatgatgagttgggtccgccaggctccagggaaggggctggaatgggtctcaactattcgtgccagtggtgataggacacactatgcagactccgtgaggGggccgcttcaccatctccagagacaattccaagaacacgctgcatttgcaaatgtacagcctgagagtcgaggacacggccgtatactactgtatgaacgaactcggatattgtagtggtggggggggccagggagccctggtctccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcaccccggccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA unmatched, IGA1 57 89 [277, 278] c87>t|t109>c,Y37>H(+ - -)|g112>a,A38>M(+ - -)|c113>t,A38>M(+ - -)|c114>g,A38>M(+ - -)|c120>t|g153>a|g163>a,A55>T(- - -)|a169>c,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g188>a,G63>D(- - -)|c192>g,S64>R(- - -)|t196>c,Y66>H(+ - -)|c201>t|a215>g,K72>R(+ + +)|g225>c|t262>c,Y88>H(+ - -)|c265>t|a274>t,N92>Y(- - -)|c287>t,A96>V(+ - +)|t306>c|g313>a,A105>M(+ - -)|c314>t,A105>M(+ - -)| c87>t|t109>c,Y37>H(+ - -)|g112>a,A38>M(+ - -)|c113>t,A38>M(+ - -)|c114>g,A38>M(+ - -)| c120>t|g153>a|g163>a,A55>T(- - -)| a169>c,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g188>a,G63>D(- - -)|c192>g,S64>R(- - -)| t196>c,Y66>H(+ - -)|c201>t|a215>g,K72>R(+ + +)|g225>c|t262>c,Y88>H(+ - -)|c265>t|a274>t,N92>Y(- - -)|c287>t,A96>V(+ - +)|t306>c| g313>a,A105>M(+ - -)|c314>t,A105>M(+ - -)|a318>c,K106>N(+ - -)| 320 223 198 25 7 18 1 5 7 5 2 0 1 0 2 2 0 0 78 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 2 1 0 0 0 0 0 1 0 0 51 51 48 3 2 1 0 2 1 0 0 0 0 0 0 0 0 0 30 24 19 5 0 5 0 1 0 1 1 0 0 0 1 1 0 0 117 114 105 9 4 5 1 0 3 3 0 0 1 0 1 0 0 0 8 8 5 3 0 3 0 1 1 0 1 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ct ggattcacttttagcagccatatg atgagttgggtccgccaggctccagggaaggggctggaatgggtctcaact attcgtgccagtggtgataggaca cactatgcagactccgtgaggggccgcttcaccatctccagagacaattccaagaacacgctgcatttgcaaatgtacagcctgagagtcgaggacacggccgtatactactgt atgaacgaactcggatattgtagtggtggg MNELGYCSGG IGHV3-23 IGHD6-19 IGHJ4 ggattcacttttagcagccatatg atgagttgggtccgccaggctccagggaaggggctggaatgggtctcaact attcgtgccagtggtgataggaca cactatgcagactccgtgaggggccgcttcaccatctccagagacaattccaagaacacgctgcatttgcaaatgtacagcctgagagtcgaggacacggccgtatactactgt atgaacgaactcggatattgtagtggtggg unmatched 22 213 16 6 10 14 6 8 6 1 6 9 JY8QFUQ01AW6B3 14028 productive Homsap IGHV3-7*01 F 959 93.58 204/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARETLPLGVGGFDNW in-frame + cctctggattcacatttagtaattattggatgatctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaaccaagatggaggtgacatggcctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactctctgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgtgcgagagagacactcccccttggtgttgggggttttgacaactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccgatcggtcttccccctggcgccctggctcccaggagcacctccgagagccacagcggccctgggcctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG2 57 62 [293, 295] c87>a|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g119>t,S40>I(- - -)|g171>c,K57>N(+ - -)|a187>g,S63>G(+ + -)|g192>c,E64>D(+ - +)|a194>t,K65>M(- + -)|a195>g,K65>M(- + -)|t196>g,Y66>A(- - -)|a197>c,Y66>A(- - -)|a258>t|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| c87>a|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g119>t,S40>I(- - -)| g171>c,K57>N(+ - -)|a187>g,S63>G(+ + -)|g192>c,E64>D(+ - +)|a194>t,K65>M(- + -)|a195>g,K65>M(- + -)| t196>g,Y66>A(- - -)|a197>c,Y66>A(- - -)|a258>t|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 226 212 14 2 12 2 3 1 0 1 1 2 0 2 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 0 0 1 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 30 24 19 5 0 5 2 0 0 0 0 0 1 0 2 0 0 0 117 114 109 5 1 4 0 2 0 0 1 0 1 0 0 0 0 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacatttagtaattattgg atgatctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaccaagatggaggtgacatg gcctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactctctgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgt gcgagagagacactcccccttggtgttgggggttttgacaac ARETLPLGVGGFDN IGHV3-7 IGHD3-10 IGHJ4 ggattcacatttagtaattattgg atgatctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaccaagatggaggtgacatg gcctatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactctctgtatctgcaaatgaacagcctgagagccgaggacacggctatatattactgt gcgagagagacactcccccttggtgttgggggttttgacaac unmatched 14 213 6 8 4 8 2 6 1 1 5 7 JY8QFUQ01AWTQR 6264 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F (see comment) 890 90.7 195/215 nt NA Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD5-18*01 F 3 10 7 17 10.7.17 [1.17.38.11] CAGEQMIDAVSHNYAMDVW in-frame + The submitted sequence and the closest germline V-GENE allele show different CDR1-IMGT amino acid lengths (10 AA in JY8QFUQ01AWTQR ; 8 AA in Homsap_IGHV4-59*01) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tctctggtggctccatcagcatcaatacttacttctggagttggatccggcagcccccagggaagggactggagtggattgggtatatctctcacagtgggagtgccaactacaacccctccctcgagagtcgagtcaccatcttaagagacacgtccaagaaccagttctctctgaggctgagggctgtaaccgcggcggacacggccgtgtatttctgtgcgggggaacagatgattgacgcagtctcccacaactatgccatggacgtctggggccaagggaccacggtcatcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA unmatched, IGA2 57 89 [303] g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)|a113>t,Y38>F(- + -)|c120>t|a170>c,Y57>S(+ - -)|t172>c,Y58>H(+ - -)|c192>t|a193>g,T65>A(- - -)|a214>g,K72>E(+ - -)|a234>c|c236>t,S79>L(- - -)|g238>a,V80>R(- - -)|t239>g,V80>R(- - -)|c264>t|a269>g,K90>R(+ + +)|c276>g,S92>R(- - -)|t277>g,S93>A(- + -)|g282>a|t288>g|a308>t,Y103>F(- + -)|a316>g,R106>G(- - -)|a318>g,R106>G(- - -)| g104>a,S35>N(- - -)|g107>c,S36>T(+ - +)|a113>t,Y38>F(- + -)| c120>t| a170>c,Y57>S(+ - -)|t172>c,Y58>H(+ - -)|c192>t|a193>g,T65>A(- - -)| a214>g,K72>E(+ - -)|a234>c|c236>t,S79>L(- - -)|g238>a,V80>R(- - -)|t239>g,V80>R(- - -)|c264>t|a269>g,K90>R(+ + +)|c276>g,S92>R(- - -)|t277>g,S93>A(- + -)|g282>a|t288>g|a308>t,Y103>F(- + -)| a316>g,R106>G(- - -)|a318>g,R106>G(- - -)| 320 229 207 22 6 16 5 3 4 1 2 0 2 0 1 1 0 3 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 0 1 0 0 0 0 1 0 1 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 21 17 4 1 3 1 0 1 1 1 0 0 0 0 0 0 0 117 114 102 12 4 8 2 2 2 0 1 0 1 0 0 1 0 3 8 8 6 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcatcaatacttacttc tggagttggatccggcagcccccagggaagggactggagtggattgggtat atctctcacagtgggagtgcc aactacaacccctccctcgagagtcgagtcaccatcttaagagacacgtccaagaaccagttctctctgaggctgagggctgtaaccgcggcggacacggccgtgtatttctgt gcgggggaacagatgattgacgcagtctcccacaactatgccatggacgtc AGEQMIDAVSHNYAMDV IGHV4-59 IGHD5-18 IGHJ6 ggtggctccatcagcatcaatacttacttc tggagttggatccggcagcccccagggaagggactggagtggattgggtat atctctcacagtgggagtgcc aactacaacccctccctcgagagtcgagtcaccatcttaagagacacgtccaagaaccagttctctctgaggctgagggctgtaaccgcggcggacacggccgtgtatttctgt gcgggggaacagatgattgacgcagtctcccacaactatgccatggacgtc unmatched 20 216 11 9 7 9 4 11 5 1 8 6 JY8QFUQ01AY4JM 6231 productive Homsap IGHV3-66*01 F, or Homsap IGHV3-66*02 F or Homsap IGHV3-66*04 F 948 93.93 201/214 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD4-23*01 ORF 2 8 7 10 8.7.10 [1.17.38.11] CARGDGVKDDYW in-frame + ctctggattcaccgtcaatagaaactacatgagctgggtccgccaggctccagggaagggactggagtgggtctcagttatttccagcggtggttccacatactacgcaaactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtatatcttcaaatgaacagcctgagagtcgacgacacggctgtgtattactgtgcgagaggggacggggttaaggatgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccccatggcaccctcctccaagagcacctctggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG1 57 75 [276, 277] g104>a,S35>N(- - -)|c108>a,S36>R(- - -)|g147>a|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a190>t|g191>c|g205>a,D69>N(+ + -)|a223>c|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| g104>a,S35>N(- - -)|c108>a,S36>R(- - -)| g147>a| a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a190>t|g191>c| g205>a,D69>N(+ + -)|a223>c|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| a320>g| 319 (320) 221 (222) 208 13 (14) 4 (5) 9 0 (1) 4 1 1 2 1 1 0 2 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 0 1 0 0 0 0 0 0 51 51 50 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 30 21 17 4 2 2 0 0 0 1 1 0 1 0 1 0 0 0 117 114 108 6 1 5 0 2 1 0 1 0 0 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|ggca,220-223(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccgtcaatagaaactac atgagctgggtccgccaggctccagggaagggactggagtgggtctcagtt atttccagcggtggttccaca tactacgcaaactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtatatcttcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaggggacggggttaaggatgactac ARGDGVKDDY IGHV3-66 IGHD4-23 IGHJ4 ggattcaccgtcaatagaaactac atgagctgggtccgccaggctccagggaagggactggagtgggtctcagtt atttccagcggtggttccaca tactacgcaaactccgtgaagggccgattcaccatctccagagacaattccaagaacacggtatatcttcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaggggacggggttaaggatgactac unmatched 13 210 6 7 5 9 1 4 2 2 5 4 JY8QFUQ01B0222 14955 productive Homsap IGHV4-34*01 F, or Homsap IGHV4-34*02 F or Homsap IGHV4-34*12 F (see comment) 782 84.65 182/215 nt NA Homsap IGHJ5*02 F 192 86.27 44/51 nt Homsap IGHD2-15*01 F 2 8 7 18 8.7.18 [1.17.38.11] CARGEECRSGSCYSVWFDPW in-frame + Low V-REGION identity (84,65% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tgtctggtgggtccttaagtgactcctactgggcctggatccgccagcccccagggaagggcctggagtacattggggagatcagtcatgatggtagaaccatgttcaattggtccctcaagagtcgactcaccatctcagtagacacgtccaagaatcaattctccctgagattgacctctgtgaccgccgcggacacggctgtttattactgcgcgagaggcgaggaatgtcggagtggaagttgctactccgtttggttcgacccctggggccagggagcccaagtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [300, 301] c75>g|a77>c,Y26>S(+ - -)|c90>a,F30>L(+ - -)|g107>a,G36>D(- - -)|t108>c,G36>D(- - -)|a110>c,Y37>S(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g147>c|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|a165>g|a170>g,N57>S(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|a189>t|c192>a,S64>R(- - -)|a197>t,N66>M(- - -)|c198>g,N66>M(- - -)|a200>t,Y67>F(- + -)|c204>t|c205>t,P69>W(- - -)|c206>g,P69>W(- - -)|g226>c,V76>L(+ - +)|a234>c|c255>t|g258>a|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c271>t|g275>c,S92>T(+ - +)|g303>t|t312>c| c75>g|a77>c,Y26>S(+ - -)| c90>a,F30>L(+ - -)|g107>a,G36>D(- - -)|t108>c,G36>D(- - -)|a110>c,Y37>S(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g147>c|g155>a,W52>Y(- + -)|g156>c,W52>Y(- + -)|a165>g| a170>g,N57>S(- - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|a189>t|c192>a,S64>R(- - -)| a197>t,N66>M(- - -)|c198>g,N66>M(- - -)|a200>t,Y67>F(- + -)|c204>t|c205>t,P69>W(- - -)|c206>g,P69>W(- - -)|g226>c,V76>L(+ - +)|a234>c|c255>t|g258>a|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|c271>t|g275>c,S92>T(+ - +)|g303>t|t312>c| 320 223 190 33 11 22 5 5 4 2 3 2 3 0 5 3 1 0 78 5 3 2 1 1 0 0 0 0 1 0 0 0 0 1 0 0 36 24 20 4 0 4 0 1 0 1 1 1 0 0 0 0 0 0 51 51 45 6 2 4 2 1 0 0 0 0 0 0 3 0 0 0 30 21 16 5 1 4 2 1 0 0 0 1 1 0 0 0 0 0 117 114 98 16 7 9 1 2 4 1 1 0 2 0 2 2 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,11-12(FR1)|aa,40-41(FR1)|ta,76-77(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,189-190(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,11-12(FR1)|tt,37-38(FR1)|tt,45-46(FR1)|ta,76-77(FR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,14-17(FR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tgtct ggtgggtccttaagtgactcctac tgggcctggatccgccagcccccagggaagggcctggagtacattggggag atcagtcatgatggtagaacc atgttcaattggtccctcaagagtcgactcaccatctcagtagacacgtccaagaatcaattctccctgagattgacctctgtgaccgccgcggacacggctgtttattactgc gcgagaggcgaggaatgtcggagtggaagttgctactccgtttggttcgacccc ARGEECRSGSCYSVWFDP IGHV4-34 IGHD2-15 IGHJ5 ggtgggtccttaagtgactcctac tgggcctggatccgccagcccccagggaagggcctggagtacattggggag atcagtcatgatggtagaacc atgttcaattggtccctcaagagtcgactcaccatctcagtagacacgtccaagaatcaattctccctgagattgacctctgtgaccgccgcggacacggctgtttattactgc gcgagaggcgaggaatgtcggagtggaagttgctactccgtttggttcgacccc unmatched 31 210 16 15 9 19 7 12 9 1 13 8 JY8QFUQ01B19RC 8013 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 990 95.39 207/217 nt NA Homsap IGHJ4*02 F (see comment) 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 8 19 8.8.19 [1.17.38.11] CAKDGQRYCSNGVCSSFFDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcacctacagcagctatgccatgagctgggtccgccaggctccagggaaggggctggagtgggtctcagcaattagtggtggtggtgctagtacataccacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatttgcaaatgaacagcctgagagccgacgacacggccgtatattactgtgcgaaagatggacaacgatattgctctaatggtgtatgctcgtccttctttgactcctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggccccatcggtcttccccctggcaccctctccaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccggaaccggtgacggtgtcgtggaactc NA unmatched, IGG1 64 75 [306, 307, 302] t89>a,F30>Y(- + -)|t90>c,F30>Y(- + -)|t165>a|a175>g,S59>G(+ + -)|g188>c,G63>A(- + -)|c192>t|t199>c,Y67>H(+ - -)|g225>a|c265>t|g291>c,E97>D(+ - +)| t89>a,F30>Y(- + -)|t90>c,F30>Y(- + -)| t165>a| a175>g,S59>G(+ + -)|g188>c,G63>A(- + -)|c192>t| t199>c,Y67>H(+ - -)|g225>a|c265>t|g291>c,E97>D(+ - +)| 320 225 215 10 4 6 1 1 2 2 0 0 0 2 2 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 1 0 0 0 1 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 24 21 3 1 2 1 0 1 0 0 0 0 0 1 0 0 0 117 114 110 4 2 2 0 1 1 1 0 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctacagcagctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagca attagtggtggtggtgctagtaca taccacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatttgcaaatgaacagcctgagagccgacgacacggccgtatattactgt gcgaaagatggacaacgatattgctctaatggtgtatgctcgtccttctttgactcc AKDGQRYCSNGVCSSFFDS IGHV3-23 IGHD2-8 IGHJ4 ggattcacctacagcagctatgcc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagca attagtggtggtggtgctagtaca taccacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatttgcaaatgaacagcctgagagccgacgacacggccgtatattactgt gcgaaagatggacaacgatattgctctaatggtgtatgctcgtccttctttgactcc unmatched 10 213 6 4 3 5 3 5 3 1 2 4 JY8QFUQ01B1HC5 18434 productive Homsap IGHV4-4*02 F, or Homsap IGHV4-4*03 F or Homsap IGHV4-4*04 F or Homsap IGHV4-4*05 F 950 93.12 203/218 nt NA Homsap IGHJ1*01 F 224 92.31 48/52 nt Homsap IGHD4-23*01 ORF 2 9 7 10 9.7.10 [1.17.38.11] CASHGGKYFPQW in-frame + tctctggtggctccatcagcagtagtaactggtggagttgggtccgccagcccccagggaaggggctggagtggattggacaaatccatcatggtgggggcaccaattacaacccgtccctcgagagtcgagtcactatatcagtagacaagtccaagaaccacctctccctgaccctgaactctgtgaccgccgcggacacggccgtttatcactgtgcgagccacggtggtaaatacttcccgcagtggggccagggcaccctggtcaccgtctcctcagcatccccggaccagccccaaggtcttcccgctgagcctcgacagcacccccctaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccgag NA unmatched, IGA2 57 84 [280, 281] g162>a|g163>c,E55>Q(+ + -)|t169>c,Y57>H(+ - -)|a175>g,S59>G(+ + -)|a190>g,S64>G(+ + -)|c198>t|a214>g,K72>E(+ - -)|c231>t|g258>c,Q86>H(- + -)|t259>c,F87>L(+ - -)|a269>c,K90>T(- - -)|g270>c,K90>T(- - -)|g275>a,S92>N(- - -)|g303>t|t307>c,Y103>H(+ - -)| g162>a|g163>c,E55>Q(+ + -)| t169>c,Y57>H(+ - -)|a175>g,S59>G(+ + -)|a190>g,S64>G(+ + -)| c198>t|a214>g,K72>E(+ - -)|c231>t|g258>c,Q86>H(- + -)|t259>c,F87>L(+ - -)|a269>c,K90>T(- - -)|g270>c,K90>T(- - -)|g275>a,S92>N(- - -)|g303>t|t307>c,Y103>H(+ - -)| a318>c,R106>S(- - -)|g319>c| 317 (320) 223 (226) 208 (209) 15 (17) 4 (5) 11 (12) 3 2 2 3 1 (2) 0 0 0 3 (4) 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 27 27 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 30 21 18 3 0 3 2 0 0 1 0 0 0 0 0 0 0 0 117 114 104 10 3 7 1 1 2 2 1 0 0 0 2 0 1 0 5 (8) 5 5 (6) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcagtagtaactgg tggagttgggtccgccagcccccagggaaggggctggagtggattggacaa atccatcatggtgggggcacc aattacaacccgtccctcgagagtcgagtcactatatcagtagacaagtccaagaaccacctctccctgaccctgaactctgtgaccgccgcggacacggccgtttatcactgt gcgagccacggtggtaaatacttcccgcag ASHGGKYFPQ IGHV4-4 IGHD4-23 IGHJ1 ggtggctccatcagcagtagtaactgg tggagttgggtccgccagcccccagggaaggggctggagtggattggacaa atccatcatggtgggggcacc aattacaacccgtccctcgagagtcgagtcactatatcagtagacaagtccaagaaccacctctccctgaccctgaactctgtgaccgccgcggacacggccgtttatcactgt gcgagccacggtggtaaatacttcccgcag unmatched 15 213 10 5 4 8 6 7 4 0 8 3 JY8QFUQ01B1QV9 14376 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 783 84.79 184/217 nt NA Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD3-10*02 F 2 8 8 20 8.8.20 [1.17.38.11] CTKTRVVLGITFPFYIYGLDVW in-frame + Low V-REGION identity (84,79% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctggattcacgttcgacaactatgccatgagctgggtccgccaggcaccaggaaaggggctggagtgggtctccagtattagtggtaatggagaaattgtacaccacgcagacgccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtttttgcaaatgaatggagtgagagacgacgacacggccatttactactgtacgaaaacacgcgttgtgttgggaattacatttccgttctatatctatggtctggacgtctggggccaagggaccacggtcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccc NA unmatched, IGA2 57 89 [308, 309] c87>g|t90>c|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)|t135>a|g141>a|a162>c|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|g176>a,S59>N(- - -)|t186>a|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>t,S64>I(- - -)|c192>t,S64>I(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|t196>c,Y66>H(+ - -)|t199>c,Y67>H(+ - -)|t208>g,S70>A(- + -)|t246>c|a263>t,Y88>F(- + -)|c265>t|c276>t|a277>g,S93>G(+ + -)|c279>a,S93>G(+ + -)|c280>g,L94>V(+ - +)|c287>a,A96>D(- - -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)|t306>c|g313>a,A105>T(- - -)| c87>g|t90>c|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)| t135>a|g141>a|a162>c|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| g176>a,S59>N(- - -)|t186>a|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|g191>t,S64>I(- - -)|c192>t,S64>I(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)| t196>c,Y66>H(+ - -)|t199>c,Y67>H(+ - -)|t208>g,S70>A(- + -)|t246>c|a263>t,Y88>F(- + -)|c265>t|c276>t|a277>g,S93>G(+ + -)|c279>a,S93>G(+ + -)|c280>g,L94>V(+ - +)|c287>a,A96>D(- - -)|g291>c,E97>D(+ - +)|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)|t306>c| g313>a,A105>T(- - -)|g319>a|a320>c| 318 (320) 223 (225) 189 34 (36) 10 (12) 24 3 8 (9) 4 5 1 (2) 2 2 3 1 3 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 1 2 0 1 0 0 0 0 0 1 0 0 51 51 46 5 3 2 0 2 0 0 1 0 0 1 0 1 0 0 30 24 16 8 1 7 1 2 2 0 0 0 0 2 0 0 1 0 117 114 99 15 4 11 1 1 2 4 0 2 2 0 1 1 0 1 6 (8) 6 5 1 (3) 0 (2) 1 0 1 (2) 0 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacgttcgacaactatgcc atgagctgggtccgccaggcaccaggaaaggggctggagtgggtctccagt attagtggtaatggagaaattgta caccacgcagacgccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtttttgcaaatgaatggagtgagagacgacgacacggccatttactactgt acgaaaacacgcgttgtgttgggaattacatttccgttctatatctatggtctggacgtc TKTRVVLGITFPFYIYGLDV IGHV3-23 IGHD3-10 IGHJ6 ggattcacgttcgacaactatgcc atgagctgggtccgccaggcaccaggaaaggggctggagtgggtctccagt attagtggtaatggagaaattgta caccacgcagacgccgtgaagggccggttcaccatctccagagacaactccaagaacacgctgtttttgcaaatgaatggagtgagagacgacgacacggccatttactactgt acgaaaacacgcgttgtgttgggaattacatttccgttctatatctatggtctggacgtc unmatched 33 213 19 14 11 18 8 15 7 3 13 10 JY8QFUQ01B1S6W 18603 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 935 93.02 200/215 nt NA Homsap IGHJ5*02 F 111 68.63 35/51 nt Homsap IGHD4-11*01 ORF 2 8 7 17 8.7.17 [1.17.38.11] CARVGRSVGYSDSGWFDPW in-frame + tctctggtggctccatgagtagttactactggaactggattcggcagcccccagggaagggactggagtggattgggtatatctattacactgggatcaccaactacaatccctccctcaagagtcgagtcaccatgtcaatagacacgtccaggaagcagttctccctgaccctgacctctgtgaccgctgcggacacggccgtctatttctgtgcgagagtcggccggagtgtgggctacagtgactctgggtggttcgacccctggggccagggaaccctggtaccgtctcctacccatcccgaccagccccaaggtcttcccgctgagccctctgcagcacccgagccagatgggaacgtggtcatcgcctgcctggtccagggccttcttcccccag NA unmatched, IGA1 64 84 [295, 297] c90>g,I30>M(+ + -)|g119>a,S40>N(- - -)|c126>t|g176>c,S59>T(+ - +)|g191>t,S64>I(- - -)|c204>t|a234>g,I78>M(+ + -)|g238>a,V80>I(+ - +)|a251>g,K84>R(+ + +)|c255>g,N85>K(+ - -)|a269>c,K90>T(- - -)|g270>c,K90>T(- - -)|g275>c,S92>T(+ - +)|g303>c|a308>t,Y103>F(- + -)| c90>g,I30>M(+ + -)| g119>a,S40>N(- - -)|c126>t| g176>c,S59>T(+ - +)|g191>t,S64>I(- - -)| c204>t|a234>g,I78>M(+ + -)|g238>a,V80>I(+ - +)|a251>g,K84>R(+ + +)|c255>g,N85>K(+ - -)|a269>c,K90>T(- - -)|g270>c,K90>T(- - -)|g275>c,S92>T(+ - +)|g303>c|a308>t,Y103>F(- + -)| a320>t| 319 (320) 222 (223) 207 15 (16) 3 (4) 12 2 2 2 0 1 0 1 (2) 0 4 2 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 49 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 30 21 19 2 0 2 0 0 0 0 0 0 0 0 1 0 1 0 117 114 104 10 2 8 2 1 1 0 1 0 1 0 3 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatgagtagttactac tggaactggattcggcagcccccagggaagggactggagtggattgggtat atctattacactgggatcacc aactacaatccctccctcaagagtcgagtcaccatgtcaatagacacgtccaggaagcagttctccctgaccctgacctctgtgaccgctgcggacacggccgtctatttctgt gcgagagtcggccggagtgtgggctacagtgactctgggtggttcgacccc ARVGRSVGYSDSGWFDP IGHV4-59 IGHD4-11 IGHJ5 ggtggctccatgagtagttactac tggaactggattcggcagcccccagggaagggactggagtggattgggtat atctattacactgggatcacc aactacaatccctccctcaagagtcgagtcaccatgtcaatagacacgtccaggaagcagttctccctgaccctgacctctgtgaccgctgcggacacggccgtctatttctgt gcgagagtcggccggagtgtgggctacagtgactctgggtggttcgacccc unmatched 15 210 6 9 4 11 2 4 3 0 9 3 JY8QFUQ01B2ACS 13976 productive Homsap IGHV3-11*01 F 1008 96.31 209/217 nt NA Homsap IGHJ5*02 F (see comment) 165 80.39 41/51 nt Homsap IGHD2-2*01 F 2 8 8 12 8.8.12 [1.17.38.11] CAVDCTSISCNGDW in-frame + Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctgggttcaccttcagtgactactacatgagctggatccgccaggctccagggaaggggctggagtggctttcatatattagtagtgctggtagtaccatatactacgcagactctgtgaggggccgattcaccatctccagggacaacaccaagaactcactgtatctgcaaatgaacagcctgagggccgaggacacggccgtgtattactgtgcggtagattgtactagtatcagctgcaacggagactggggccagggaaccctggtcaccgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctcgacacgcacccccaagcatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 43 74 [284, 285] a81>g|g157>c,V53>L(+ - +)|c165>t|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|a215>g,K72>R(+ + +)|g247>a,A83>T(- - -)|a285>g|a316>g,R106>V(- - -)|g317>t,R106>V(- - -)| a81>g| g157>c,V53>L(+ - +)|c165>t| a175>g,S59>A(- + -)|g176>c,S59>A(- + -)| a215>g,K72>R(+ + +)|g247>a,A83>T(- - -)|a285>g| a316>g,R106>V(- - -)|g317>t,R106>V(- - -)| 320 225 215 10 3 7 5 1 1 0 0 0 0 0 2 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 0 1 0 0 0 0 0 1 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 1 0 0 0 117 114 111 3 1 2 2 1 0 0 0 0 0 0 0 0 0 0 8 8 6 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcaccttcagtgactactac atgagctggatccgccaggctccagggaaggggctggagtggctttcatat attagtagtgctggtagtaccata tactacgcagactctgtgaggggccgattcaccatctccagggacaacaccaagaactcactgtatctgcaaatgaacagcctgagggccgaggacacggccgtgtattactgt gcggtagattgtactagtatcagctgcaacggagac AVDCTSISCNGD IGHV3-11 IGHD2-2 IGHJ5 gggttcaccttcagtgactactac atgagctggatccgccaggctccagggaaggggctggagtggctttcatat attagtagtgctggtagtaccata tactacgcagactctgtgaggggccgattcaccatctccagggacaacaccaagaactcactgtatctgcaaatgaacagcctgagggccgaggacacggccgtgtattactgt gcggtagattgtactagtatcagctgcaacggagac unmatched 8 213 6 2 2 4 4 4 2 1 3 2 JY8QFUQ01B2E25 4828 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 962 94.42 203/215 nt 93.95 202/215 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 7 8 8.7.8 [1.17.38.11] CARDNYGDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 63 of V-REGION: 1 nucleotides (from position 94 in the user submitted sequence), (cause frameshift) tctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgtgttaatggtgatggggtaggacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccaagcatgggaacgtggtccgtcgcctgcctggtccagggcttcttccccca NA unmatched, IGA2 57 84 [270] g153>t|a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|c192>g,S64>R(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| g153>t| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|c192>g,S64>R(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|t300>a|g301>a,V101>I(+ - +)|g303>a,V101>I(+ - +)| 320 223 211 12 5 7 3 2 0 0 0 0 0 1 3 1 2 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 30 23 20 3 0 3 2 0 0 0 0 0 0 0 0 1 0 0 117 114 106 8 4 4 1 2 0 0 0 0 0 1 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgt gttaatggtgatggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtttgggtctcacgt gttaatggtgatggggtaggaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggcaatatattactgt gcaagagataattacggtgactac unmatched 12 212 5 7 2 8 3 4 5 0 4 3 JY8QFUQ01B2GL4 12972 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 902 90.05 199/221 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-18*01 F 2 10 7 12 10.7.12 [1.17.38.11] CARHSRLVRYFEFW in-frame + tctctggtggctccatcaacagtggtagttatcactgggcctggatccgccagtccccagggaaggggctggagtggattgggagcgtctcctatggtgggaacacctactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgtgcgagacattcgagactggtacggtactttgaattctggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacacgcacccccaagactgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA unmatched, IGA2 43 74 [288, 289] g92>a,S31>N(- - -)|a103>g,S35>G(+ + -)|c111>t|t112>c,Y38>H(+ - -)|g119>c,G40>A(- + -)|c133>t,P45>S(+ - -)|t165>c|a166>g,I56>V(+ - +)|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a175>g,S59>G(+ + -)|g191>a,S64>N(- - -)|a215>t,K72>M(- + -)|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|c236>t,S79>F(- - -)|a240>c|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c261>g,F87>L(+ - -)|c271>g,L91>V(+ - +)|a291>g| g92>a,S31>N(- - -)|a103>g,S35>G(+ + -)|c111>t|t112>c,Y38>H(+ - -)| g119>c,G40>A(- + -)|c133>t,P45>S(+ - -)|t165>c| a166>g,I56>V(+ - +)|a170>c,Y57>S(+ - -)|t171>c,Y57>S(+ - -)|a175>g,S59>G(+ + -)|g191>a,S64>N(- - -)| a215>t,K72>M(- + -)|a229>g,T77>D(- + -)|c230>a,T77>D(- + -)|c236>t,S79>F(- - -)|a240>c|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|c261>g,F87>L(+ - -)|c271>g,L91>V(+ - +)|a291>g| 320 229 207 22 4 18 6 2 4 3 2 1 1 0 1 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 1 3 1 1 1 1 0 0 0 0 0 0 0 0 51 51 48 3 1 2 0 0 1 1 0 0 0 0 1 0 0 0 30 21 16 5 0 5 2 1 0 1 1 0 0 0 0 0 0 0 117 114 104 10 2 8 3 0 2 0 1 1 1 0 0 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcaacagtggtagttatcac tgggcctggatccgccagtccccagggaaggggctggagtggattgggagc gtctcctatggtgggaacacc tactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgt gcgagacattcgagactggtacggtactttgaattc ARHSRLVRYFEF IGHV4-39 IGHD5-18 IGHJ4 ggtggctccatcaacagtggtagttatcac tgggcctggatccgccagtccccagggaaggggctggagtggattgggagc gtctcctatggtgggaacacc tactacaacccgtccctcatgagtcgagtcgacatattcgtcgacacgtccaagagtcagttgtccctgaaggtgagctctgtgaccgccgcggacacggctgtgtattactgt gcgagacattcgagactggtacggtactttgaattc unmatched 22 216 15 7 6 10 9 12 3 1 10 8 JY8QFUQ01B2XE6 21333 productive (see comment) Homsap IGHV4-4*02 F, or Homsap IGHV4-4*03 F or Homsap IGHV4-4*04 F or Homsap IGHV4-4*05 F or Homsap IGHV4-55*03 P 815 86.24 188/218 nt 85.32 186/218 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-22*01 F 2 9 7 15 9.7.15 [1.17.38.11] CTSREHAFSGQFTGDYW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'TCA' (3 nucleotides at position 25 to 27 in the user submitted sequence), (do not cause frameshift);in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 55 to 55 in the user submitted sequence), (cause frameshift); tgtctggtgtctccgtcaccagcgTCAgtcactggtggacctgggtccgccagcCccccagggaagggactggagtggattggagaaatctattattatggcatcaccaatttcaacccgtccctcaagagtcgaatcagcatgtcagtggacgagtccaagaaccagttctccctgagactgacttctgttaccgccgcggacacggccgtttattattgtacgagtcgcgaacatgcttttagtggtcaattcacgggtgactactggggccagggaaccctgatgaccgtcacctcagcctccaccaagggccccatcggtcttcccccctggcgccctgctccaggagcacctccgagacgcacagcggcccctggggctgccctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA unmatched, IGG2 64 81 [301, 300] c75>g|g83>t,G28>V(- - -)|a88>g,I30>V(+ - +)|g92>c,S31>T(+ - +)|t105>c|a106>g,S36>G(+ + -)|a109>c,N37>H(- - -)|g119>c,S40>T(+ - +)|t120>c,S40>T(+ - +)|g147>a|g162>a|c172>t,H58>Y(+ - -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|g189>c|g191>t,S64>I(- - -)|c198>t|a200>t,Y67>F(- + -)|g226>a,V76>I(+ - +)|c230>g,T77>S(+ - +)|a234>g,I78>M(+ + -)|a240>g|a244>g,K82>E(+ - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g282>t|g303>t|c309>t|g313>a,A105>T(- - -)| c75>g| g83>t,G28>V(- - -)|a88>g,I30>V(+ - +)|g92>c,S31>T(+ - +)|t105>c|a106>g,S36>G(+ + -)|a109>c,N37>H(- - -)| g119>c,S40>T(+ - +)|t120>c,S40>T(+ - +)|g147>a|g162>a| c172>t,H58>Y(+ - -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|g189>c|g191>t,S64>I(- - -)| c198>t|a200>t,Y67>F(- + -)|g226>a,V76>I(+ - +)|c230>g,T77>S(+ - +)|a234>g,I78>M(+ + -)|a240>g|a244>g,K82>E(+ - -)|a269>g,K90>R(+ + +)|g270>a,K90>R(+ + +)|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g282>t|g303>t|c309>t| g313>a,A105>T(- - -)|a318>t,R106>S(- - -)|g319>c|a320>g| 317 (320) 223 (226) 192 31 (34) 10 (12) 21 (22) 6 (7) 6 4 2 1 0 2 (3) 0 4 (5) 2 4 0 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 27 21 6 1 5 2 0 0 1 1 0 0 0 1 0 1 0 51 51 47 4 2 2 0 2 0 1 0 0 0 0 1 0 0 0 30 21 16 5 1 4 0 1 1 0 0 0 1 0 1 0 1 0 117 114 100 14 5 9 4 2 3 0 0 0 1 0 1 1 2 0 5 (8) 5 4 1 (4) 0 (2) 1 (2) 0 (1) 1 0 0 0 0 0 (1) 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tgtct ggtgtctccgtcaccagcggtcactgg tggacctgggtccgccagcccccagggaagggactggagtggattggagaa atctattattatggcatcacc aatttcaacccgtccctcaagagtcgaatcagcatgtcagtggacgagtccaagaaccagttctccctgagactgacttctgttaccgccgcggacacggccgtttattattgt acgagtcgcgaacatgcttttagtggtcaattcacgggtgactac TSREHAFSGQFTGDY IGHV4-4 IGHD3-22 IGHJ4 ggtgtctccgtcaccagcggtcactgg tggacctgggtccgccagcccccagggaagggactggagtggattggagaa atctattattatggcatcacc aatttcaacccgtccctcaagagtcgaatcagcatgtcagtggacgagtccaagaaccagttctccctgagactgacttctgttaccgccgcggacacggccgtttattattgt acgagtcgcgaacatgcttttagtggtcaattcacgggtgactac unmatched 29 213 17 12 9 18 8 11 7 2 11 9 JY8QFUQ01B2XKD 7200 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 882 89.86 195/217 nt 89.4 194/217 nt Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-10*01 F 3 8 8 10 8.8.10 [1.17.38.11] CSKDTFGREDYW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 36 of V-REGION: 'A' (1 nucleotides at position 21 to 21 in the user submitted sequence), (cause frameshift); ctctggattctccgtcagtaAgttactggatgcactgggtccgccaggctccaggggaggggctggagtgggtctcacgtattaatgaagatggtagtcggacagactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgtagtaaagacacatttgggcgagaagactactggggccagggagccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagatgggaacgtggtcgtcgcctgcctggtccagggcttcttccccca NA unmatched, IGA2 64 95 [279, 278] a85>t,T29>S(+ - +)|t88>g,F30>V(+ - -)|c108>t|a132>g|a142>g,K48>E(+ - -)|t152>a,V51>E(- + -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>t|a190>c,S64>R(- - -)|c192>g,S64>R(- - -)|a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c234>t|c236>a,S79>Y(+ - -)|a245>g,N82>S(- - -)|g258>a|c287>t,A96>V(+ - +)|g291>a|g303>c|c309>t| a85>t,T29>S(+ - +)|t88>g,F30>V(+ - -)|c108>t| a132>g|a142>g,K48>E(+ - -)|t152>a,V51>E(- + -)| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>t|a190>c,S64>R(- - -)|c192>g,S64>R(- - -)| a196>g,S66>D(- - -)|g197>a,S66>D(- - -)|c234>t|c236>a,S79>Y(+ - -)|a245>g,N82>S(- - -)|g258>a|c287>t,A96>V(+ - +)|g291>a|g303>c|c309>t| g313>a,A105>S(- + -)|c314>g,A105>S(- + -)|a315>t,A105>S(- + -)|g317>a,R106>K(+ + +)| 320 225 199 26 8 18 5 6 4 0 1 1 2 2 1 2 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 1 0 0 0 1 0 0 0 0 1 51 51 48 3 1 2 2 0 0 0 0 0 0 1 0 0 0 0 30 24 18 6 1 5 1 1 0 0 1 0 0 1 0 1 1 0 117 114 104 10 5 5 2 3 3 0 0 1 0 0 1 0 0 0 8 8 4 4 0 4 0 2 0 0 0 0 1 0 0 1 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattctccgtcagtagttactgg atgcactgggtccgccaggctccaggggaggggctggagtgggtctcacgt attaatgaagatggtagtcggaca gactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgt agtaaagacacatttgggcgagaagactac SKDTFGREDY IGHV3-74 IGHD3-10 IGHJ4 ggattctccgtcagtagttactgg atgcactgggtccgccaggctccaggggaggggctggagtgggtctcacgt attaatgaagatggtagtcggaca gactacgcggactccgtgaagggccgattcaccatttacagagacagcgccaagaacacactgtatctgcaaatgaacagtctgagagtcgaagacacggctgtctattattgt agtaaagacacatttgggcgagaagactac unmatched 22 213 13 9 8 12 5 10 6 2 7 7 JY8QFUQ01B37IT 8649 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 855 88.48 192/217 nt NA Homsap IGHJ4*02 F 141 77.08 37/48 nt Homsap IGHD3-10*01 F 2 8 8 15 8.8.15 [1.17.38.11] CARDNARSYYSGVPDFW in-frame + gtctggattcaccttcagtgaccatggcatgcactgggtccgccaggctccagggaagggtctgcagtgggtggcagttgtttggcatactggagacaataaatattatgcagagtccgtgaggggccgattcaccatctccagggacaattccaagaacacactgtatctgcaaatggacgacctgagaggcgaggacacggctatgtattattgtgcgagagataatgcccggagttattattcgggcgtgcccgacttctggggccagggaaacttggtcaccgtttcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact NA unmatched, IGG1 57 100 [294] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t109>c,Y37>H(+ - -)|c141>g|g147>t|g151>c,E51>Q(+ + -)|a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|t172>c,Y58>H(+ - -)|g175>a,D59>T(- + -)|a176>c,D59>T(- + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|t189>c,S63>D(- - -)|c198>t|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|a240>g|g258>a|a274>g,N92>D(+ + -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|c287>g,A96>G(- + -)|g301>a,V101>M(+ - -)|c309>t| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|t109>c,Y37>H(+ - -)| c141>g|g147>t|g151>c,E51>Q(+ + -)| a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|t172>c,Y58>H(+ - -)|g175>a,D59>T(- + -)|a176>c,D59>T(- + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|t189>c,S63>D(- - -)| c198>t|c207>g,D69>E(+ - +)|a215>g,K72>R(+ + +)|a240>g|g258>a|a274>g,N92>D(+ + -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|c287>g,A96>G(- + -)|g301>a,V101>M(+ - -)|c309>t| 320 225 200 25 6 19 7 6 2 3 1 0 1 0 1 3 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 1 0 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 0 0 0 0 0 0 0 1 1 1 0 30 24 16 8 0 8 2 2 0 2 1 0 1 0 0 0 0 0 117 114 103 11 4 7 4 3 2 0 0 0 0 0 0 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattcaccttcagtgaccatggc atgcactgggtccgccaggctccagggaagggtctgcagtgggtggcagtt gtttggcatactggagacaataaa tattatgcagagtccgtgaggggccgattcaccatctccagggacaattccaagaacacactgtatctgcaaatggacgacctgagaggcgaggacacggctatgtattattgt gcgagagataatgcccggagttattattcgggcgtgcccgacttc ARDNARSYYSGVPDF IGHV3-33 IGHD3-10 IGHJ4 ggattcaccttcagtgaccatggc atgcactgggtccgccaggctccagggaagggtctgcagtgggtggcagtt gtttggcatactggagacaataaa tattatgcagagtccgtgaggggccgattcaccatctccagggacaattccaagaacacactgtatctgcaaatggacgacctgagaggcgaggacacggctatgtattattgt gcgagagataatgcccggagttattattcgggcgtgcccgacttc unmatched 25 213 18 7 8 13 10 12 6 0 8 11 JY8QFUQ01B5KCJ 16526 productive Homsap IGHV3-11*01 F (see comment) 788 84.86 185/218 nt NA Homsap IGHJ6*02 F 247 88.71 55/62 nt Homsap IGHD1-26*01 F 3 8 8 15 8.8.15 [1.17.38.11] CARYRGSYYDYGGVDVW in-frame + Low V-REGION identity (84,86% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release cctctggcttcagattccgtgactactacatgacgtgggtccgccaggctccagggaagggtcttgagtggctttcctccatcagcagcggtagtaataccatccactactcagactcggtgaggggccgcttcaccatctccagggacaacaccaggaactcagtggatctgcaaatgaatagtctgagagccgaagacacggccgtctattattgtgcgaggtacagaggcagttactacgactatggcggtgtggacgtctggggccaagggaccacggtcaccgtctccccagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA unmatched, IGA2 57 89 [294, 295] a81>c|c86>g,T29>R(- - -)|c87>a,T29>R(- - -)|a103>c,S35>R(- - -)|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|a124>g,I42>V(+ - +)|g147>t|g150>t|g157>c,V53>L(+ - +)|a162>c|a164>c,Y55>S(+ - -)|t168>c|t171>c|t174>c|a175>g,S59>G(+ + -)|g184>a,G62>S(+ + -)|g188>a,S63>N(- - -)|a195>c|t196>c,Y66>H(+ - -)|g202>t,A68>S(- + -)|t210>g|a215>g,K72>R(+ + +)|a225>c|g247>a,A83>T(- - -)|a251>g,K84>R(+ + +)|c259>g,L87>V(+ - +)|t262>g,Y88>D(- - -)|c276>t|c279>t|g291>a|g303>c|c309>t| a81>c|c86>g,T29>R(- - -)|c87>a,T29>R(- - -)|a103>c,S35>R(- - -)| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|a124>g,I42>V(+ - +)|g147>t|g150>t|g157>c,V53>L(+ - +)|a162>c|a164>c,Y55>S(+ - -)| t168>c|t171>c|t174>c|a175>g,S59>G(+ + -)|g184>a,G62>S(+ + -)|g188>a,S63>N(- - -)|a195>c| t196>c,Y66>H(+ - -)|g202>t,A68>S(- + -)|t210>g|a215>g,K72>R(+ + +)|a225>c|g247>a,A83>T(- - -)|a251>g,K84>R(+ + +)|c259>g,L87>V(+ - +)|t262>g,Y88>D(- - -)|c276>t|c279>t|g291>a|g303>c|c309>t| a318>g|g319>t| 317 (320) 223 (226) 190 (191) 33 (35) 15 (17) 18 4 (5) 4 3 4 6 1 0 0 3 3 3 (4) 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 0 0 2 1 0 0 0 1 0 0 51 51 43 8 3 5 1 0 0 0 2 0 0 0 2 1 2 0 30 24 17 7 4 3 1 2 0 3 1 0 0 0 0 0 0 0 117 114 100 14 7 7 2 2 3 1 1 0 0 0 1 1 1 2 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggcttcagattccgtgactactac atgacgtgggtccgccaggctccagggaagggtcttgagtggctttcctcc atcagcagcggtagtaataccatc cactactcagactcggtgaggggccgcttcaccatctccagggacaacaccaggaactcagtggatctgcaaatgaatagtctgagagccgaagacacggccgtctattattgt gcgaggtacagaggcagttactacgactatggcggtgtggacgtc ARYRGSYYDYGGVDV IGHV3-11 IGHD1-26 IGHJ6 ggcttcagattccgtgactactac atgacgtgggtccgccaggctccagggaagggtcttgagtggctttcctcc atcagcagcggtagtaataccatc cactactcagactcggtgaggggccgcttcaccatctccagggacaacaccaggaactcagtggatctgcaaatgaatagtctgagagccgaagacacggccgtctattattgt gcgaggtacagaggcagttactacgactatggcggtgtggacgtc unmatched 33 213 15 18 7 17 8 16 10 5 12 6 JY8QFUQ01B5XDW 9311 productive Homsap IGHV3-30*01 F 945 93.09 202/217 nt NA Homsap IGHJ4*01 F, or Homsap IGHJ4*03 F (see comment) 114 70.83 34/48 nt Homsap IGHD3-10*02 F 1 8 8 16 8.8.16 [1.17.38.11] CVKNDLSWGTIEHYFDYW in-frame - Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcatcttcagcaaccttgcgatgcactgggtccgccaggctccaggcaaggggctagagtgggtggcacacatatcatatgatggaaataagaaatactacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgacagctgaagacacggctatttattactgtgtgaaaaatgacctgtcttggggcacaatagagcactactttgactactgggggccagggaaccctggtcacgtctccctcagcatccccgaccagcccccaaggtcttcccgctgagcctcgcagcaccccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA unmatched, IGA1 50 79 [298, 293, 297] c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)|t109>c,Y37>L(- - -)|a110>t,Y37>L(- - -)|t114>g|g163>c,V55>H(- + -)|t164>a,V55>H(- + -)|t165>c,V55>H(- + -)|g188>a,S63>N(- - -)|t192>g,N64>K(+ - -)|g284>c,R95>T(- - -)|g291>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)|c314>t,A105>V(+ - +)| c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)|t109>c,Y37>L(- - -)|a110>t,Y37>L(- - -)|t114>g| g163>c,V55>H(- + -)|t164>a,V55>H(- + -)|t165>c,V55>H(- + -)| g188>a,S63>N(- - -)|t192>g,N64>K(+ - -)| g284>c,R95>T(- - -)|g291>a|g301>a,V101>I(+ - +)|g303>t,V101>I(+ - +)| c314>t,A105>V(+ - +)|g317>a,R106>K(+ + +)|g319>a| 316 (320) 221 (225) 205 (207) 16 (18) 3 (4) 13 (14) 0 4 (6) 2 3 0 0 1 1 2 0 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 2 4 0 1 1 2 0 0 1 0 0 0 0 1 51 51 48 3 0 3 0 0 0 1 0 0 0 1 1 0 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 117 114 110 4 1 3 0 2 0 0 0 0 0 0 1 0 1 0 4 (8) 4 3 (5) 1 (3) 0 (1) 1 (2) 0 0 (2) 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagcaaccttgcg atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcacac atatcatatgatggaaataagaaa tactacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgacagctgaagacacggctatttattactgt gtgaaaaatgacctgtcttggggcacaatagagcactactttgactac VKNDLSWGTIEHYFDY IGHV3-30 IGHD3-10 IGHJ4 ggattcatcttcagcaaccttgcg atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcacac atatcatatgatggaaataagaaa tactacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgacagctgaagacacggctatttattactgt gtgaaaaatgacctgtcttggggcacaatagagcactactttgactac unmatched 15 213 8 7 5 8 3 7 1 2 6 6 JY8QFUQ01B5ZV4 18682 productive Homsap IGHV4-61*02 F 956 92.76 205/221 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-19*01 F 1 10 7 14 10.7.14 [1.17.38.11] CAREPQSNAWYYFDHW in-frame + tctctggtggctccgtcaacagtggtaatttctactggagctggatccggcagcccgccgggaagggactggagtggatagggcgtatctatgccagtgggagcaccaactacaacccctccctcaagagtcgaatcaccatatcagcagacacatccaagaatcagttctccctgaggctgagttctgtgaccgccgcagacacaggcgtttattattgtgcgagagaaccacaaagcaatgcctggtactactttgaccactggggccggggagccctggtcaccgtctccacagcctcctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG2 7 6 [] a88>g,I30>V(+ - +)|g92>a,S31>N(- - -)|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)|t159>a|a172>g,T58>A(- - -)|g226>a,V76>I(+ - +)|t239>c,V80>A(+ - +)|g246>a|c255>t|a269>g,K90>R(+ + +)|c276>t|g297>a|c299>g,A100>G(- + -)|g303>t|c309>t| a88>g,I30>V(+ - +)|g92>a,S31>N(- - -)|g107>a,S36>N(- - -)|a110>t,Y37>F(- + -)| t159>a| a172>g,T58>A(- - -)| g226>a,V76>I(+ - +)|t239>c,V80>A(+ - +)|g246>a|c255>t|a269>g,K90>R(+ + +)|c276>t|g297>a|c299>g,A100>G(- + -)|g303>t|c309>t| 320 229 213 16 7 9 3 5 3 1 0 0 1 1 0 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 26 4 0 4 1 2 0 0 0 0 1 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 21 20 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 117 114 104 10 6 4 1 3 3 1 0 0 0 0 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcaacagtggtaatttctac tggagctggatccggcagcccgccgggaagggactggagtggatagggcgt atctatgccagtgggagcacc aactacaacccctccctcaagagtcgaatcaccatatcagcagacacatccaagaatcagttctccctgaggctgagttctgtgaccgccgcagacacaggcgtttattattgt gcgagagaaccacaaagcaatgcctggtactactttgaccac AREPQSNAWYYFDH IGHV4-61 IGHD6-19 IGHJ4 ggtggctccgtcaacagtggtaatttctac tggagctggatccggcagcccgccgggaagggactggagtggatagggcgt atctatgccagtgggagcacc aactacaacccctccctcaagagtcgaatcaccatatcagcagacacatccaagaatcagttctccctgaggctgagttctgtgaccgccgcagacacaggcgtttattattgt gcgagagaaccacaaagcaatgcctggtactactttgaccac unmatched 16 216 12 4 8 10 4 6 7 0 4 5 JY8QFUQ01B6IHX 19671 productive Homsap IGHV3-48*01 F, or Homsap IGHV3-48*02 F 851 88.07 192/218 nt NA Homsap IGHJ6*02 F 229 85.48 53/62 nt Homsap IGHD2-2*01 F 3 8 8 19 8.8.19 [1.17.38.11] CAKDRTLHTPAQMFYYGMDVW in-frame + catctggattcaccttcagttcttatagtgtaaactgggtccgccaggctccagggaagggcctagagtttgtctcatacattgatagtagtggttctaccatatactacgcagactctgtgaagggccgattcaccatctctagagacaatgcccagaactcactgtttctgcaaatgaacaacctgcgagtcgacgacacggccgtatattactgtgcaaaagaccggacacttcacacacccgctcagatgttttactatggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatctccgaccagccccaaggtcttcccgctgagcctctgcagcacccagctagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA unmatched, IGA1 57 79 [306] c75>a|a106>t|g107>c|c108>t|c114>t|a115>g,M39>V(+ - -)|g117>a,M39>V(+ - -)|g147>c|g150>a|g155>t,W52>F(+ + -)|g156>t,W52>F(+ + -)|t159>c|a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a184>g,S62>G(+ + -)|a187>t|g188>c|c237>t|a250>c,K84>Q(+ - -)|a263>t,Y88>F(- + -)|g278>a,S93>N(- - -)|a283>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t300>c|g303>a|g315>a|g317>a,R106>K(+ + +)| c75>a| a106>t|g107>c|c108>t|c114>t| a115>g,M39>V(+ - -)|g117>a,M39>V(+ - -)|g147>c|g150>a|g155>t,W52>F(+ + -)|g156>t,W52>F(+ + -)|t159>c| a169>g,S57>D(- - -)|g170>a,S57>D(- - -)|a184>g,S62>G(+ + -)|a187>t|g188>c| c237>t|a250>c,K84>Q(+ - -)|a263>t,Y88>F(- + -)|g278>a,S93>N(- - -)|a283>c|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t300>c|g303>a| g315>a|g317>a,R106>K(+ + +)| 320 226 198 28 15 13 3 7 4 2 2 1 3 0 4 0 2 0 78 5 4 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 36 24 20 4 4 0 0 0 2 0 0 0 1 0 1 0 0 0 51 51 44 7 3 4 1 2 0 1 0 0 0 0 1 0 2 0 30 24 19 5 2 3 2 1 0 0 0 0 1 0 1 0 0 0 117 114 105 9 4 5 0 2 2 1 2 0 1 0 1 0 0 0 8 8 6 2 1 1 0 2 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| catct ggattcaccttcagttcttatagt gtaaactgggtccgccaggctccagggaagggcctagagtttgtctcatac attgatagtagtggttctaccata tactacgcagactctgtgaagggccgattcaccatctctagagacaatgcccagaactcactgtttctgcaaatgaacaacctgcgagtcgacgacacggccgtatattactgt gcaaaagaccggacacttcacacacccgctcagatgttttactatggtatggacgtc AKDRTLHTPAQMFYYGMDV IGHV3-48 IGHD2-2 IGHJ6 ggattcaccttcagttcttatagt gtaaactgggtccgccaggctccagggaagggcctagagtttgtctcatac attgatagtagtggttctaccata tactacgcagactctgtgaagggccgattcaccatctctagagacaatgcccagaactcactgtttctgcaaatgaacaacctgcgagtcgacgacacggccgtatattactgt gcaaaagaccggacacttcacacacccgctcagatgttttactatggtatggacgtc unmatched 25 213 14 11 9 15 5 10 7 6 9 3 JY8QFUQ01B8YFN 19239 productive (see comment) Homsap IGHV3-7*01 F 887 89.91 196/218 nt 89.45 195/218 nt Homsap IGHJ3*02 F 196 88 44/50 nt Homsap IGHD1-1*01 F 3 8 8 10 8.8.10 [1.17.38.11] CARVNKDSTDMW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'G' (1 nucleotides at position 58 to 58 in the user submitted sequence), (cause frameshift); cctctggattcacctttagtgactattggatgaggtggttccgccagcctccaggaaGgggggctggagtgggtggccagcataaaagaagatggaagtgagaaaggttatgtggactctgtgaagggccgcttcaccatcgccagagacaacgcccagaaatcactgtttttgcagatgaacagcctgagaggcgaggacacggctgtgtatttctgtgcgagagtcaacaaggactctactgatatgtggggccaagggacaatggtcaccgtctcttcagcatccccggaccagccccgaaggtcttcccgctgagcctctgcagcacccagccagatgggaacgtgtccatcgcctgccctggtccagggcttcttcccccc NA unmatched, IGA1 86 68 [282] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c120>g,S40>R(- - -)|g124>t,V42>F(+ - -)|g133>c,A45>P(- - -)|g141>a|a143>g,K48>R(+ + +)|a164>g,N55>S(- - -)|g171>a|c172>g,Q58>E(+ + -)|t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>t,Y66>G(+ - -)|a225>c|t235>g,S79>A(- + -)|a250>c,K84>Q(+ - -)|c255>a,N85>K(+ - -)|a263>t,Y88>F(- + -)|c265>t|a270>g|c287>g,A96>G(- + -)|a308>t,Y103>F(- + -)| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| c120>g,S40>R(- - -)|g124>t,V42>F(+ - -)|g133>c,A45>P(- - -)|g141>a|a143>g,K48>R(+ + +)|a164>g,N55>S(- - -)| g171>a|c172>g,Q58>E(+ + -)| t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>t,Y66>G(+ - -)|a225>c|t235>g,S79>A(- + -)|a250>c,K84>Q(+ - -)|c255>a,N85>K(+ - -)|a263>t,Y88>F(- + -)|c265>t|a270>g|c287>g,A96>G(- + -)|a308>t,Y103>F(- + -)| a320>t| 319 (320) 225 (226) 203 22 (23) 5 (6) 17 5 3 2 0 2 1 2 (3) 0 1 3 1 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 45 6 1 5 2 1 0 0 0 0 0 0 1 1 1 0 30 24 22 2 1 1 0 1 0 0 0 0 0 0 0 1 0 0 117 114 102 12 3 9 2 0 2 0 2 1 2 0 0 1 0 2 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagtgactattgg atgaggtggttccgccagcctccaggaagggggctggagtgggtggccagc ataaaagaagatggaagtgagaaa ggttatgtggactctgtgaagggccgcttcaccatcgccagagacaacgcccagaaatcactgtttttgcagatgaacagcctgagaggcgaggacacggctgtgtatttctgt gcgagagtcaacaaggactctactgatatg ARVNKDSTDM IGHV3-7 IGHD1-1 IGHJ3 ggattcacctttagtgactattgg atgaggtggttccgccagcctccaggaagggggctggagtgggtggccagc ataaaagaagatggaagtgagaaa ggttatgtggactctgtgaagggccgcttcaccatcgccagagacaacgcccagaaatcactgtttttgcagatgaacagcctgagaggcgaggacacggctgtgtatttctgt gcgagagtcaacaaggactctactgatatg unmatched 22 213 10 12 5 11 5 11 4 1 14 3 JY8QFUQ01B9KF0 10215 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 792 85.25 185/217 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD3-10*01 F 2 8 8 15 8.8.15 [1.17.38.11] CVRVKRFGSGNVLFDYW in-frame + ctccggtttcacgtttgacaactatgccatgacttgggtccgccagactccagggaaggggctgcagtggctctcaactattactgcttatgggactctcacatactacgctgcctccgtgaagggccggttcaccctctccagggacaactccaacaacacggtgtatctgcaaatggacagtctgagagccgaagacacggccgtattttactgtgtgagagttaaacggttcggttcggggaatgtactctttgactactggggccagggaaccctggtcaccgtctcgtcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaaccgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 79 [293, 295] t78>c|a81>t|c87>g|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g133>a,A45>T(- - -)|g151>c,E51>Q(+ + -)|g157>c,V53>L(+ - +)|g163>a,A55>T(- - -)|g170>c,S57>T(+ - +)|g173>c,G58>A(- + -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|t186>g|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|a190>c,S64>L(- - -)|g191>t,S64>L(- - -)|a204>t|a206>c,D69>A(- - -)|a232>c,I78>L(+ + +)|a240>g|t246>c|g252>c,K84>N(+ - -)|c259>g,L87>V(+ - +)|a274>g,N92>D(+ + -)|c279>t|g291>a|a305>t,Y102>F(- + -)|c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)| t78>c| a81>t|c87>g|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g133>a,A45>T(- - -)|g151>c,E51>Q(+ + -)|g157>c,V53>L(+ - +)|g163>a,A55>T(- - -)| g170>c,S57>T(+ - +)|g173>c,G58>A(- + -)|a175>t,S59>Y(+ - -)|g176>a,S59>Y(+ - -)|t186>g|g187>a,G63>T(+ - -)|g188>c,G63>T(+ - -)|a190>c,S64>L(- - -)|g191>t,S64>L(- - -)| a204>t|a206>c,D69>A(- - -)|a232>c,I78>L(+ + +)|a240>g|t246>c|g252>c,K84>N(+ - -)|c259>g,L87>V(+ - +)|a274>g,N92>D(+ + -)|c279>t|g291>a|a305>t,Y102>F(- + -)| c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)|a320>t| 319 (320) 224 (225) 190 34 (35) 9 (10) 25 4 7 3 2 3 0 4 (5) 0 7 2 1 1 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 19 5 2 3 1 2 0 0 0 0 1 0 0 1 0 0 51 51 45 6 0 6 0 2 1 0 0 0 0 0 3 0 0 0 30 24 15 9 1 8 0 2 0 0 1 0 1 0 3 0 1 1 117 114 103 11 5 6 2 1 1 1 2 0 2 0 1 1 0 0 7 (8) 7 5 2 (3) 0 (1) 2 1 0 1 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggtttcacgtttgacaactatgcc atgacttgggtccgccagactccagggaaggggctgcagtggctctcaact attactgcttatgggactctcaca tactacgctgcctccgtgaagggccggttcaccctctccagggacaactccaacaacacggtgtatctgcaaatggacagtctgagagccgaagacacggccgtattttactgt gtgagagttaaacggttcggttcggggaatgtactctttgactac VRVKRFGSGNVLFDY IGHV3-23 IGHD3-10 IGHJ4 ggtttcacgtttgacaactatgcc atgacttgggtccgccagactccagggaaggggctgcagtggctctcaact attactgcttatgggactctcaca tactacgctgcctccgtgaagggccggttcaccctctccagggacaactccaacaacacggtgtatctgcaaatggacagtctgagagccgaagacacggccgtattttactgt gtgagagttaaacggttcggttcggggaatgtactctttgactac unmatched 31 213 13 18 9 19 4 12 5 3 12 11 JY8QFUQ01BAG1V 17531 productive Homsap IGHV1-3*01 F 822 87.38 187/214 nt NA Homsap IGHJ5*02 F 201 88.24 45/51 nt Homsap IGHD6-6*01 F 1 8 8 13 8.8.13 [0.17.38.11] CVRGRSPPGDWFDPW in-frame + tggatacaccttcactaattatgctctgcaatgggtgcgccgggcccccggacaaacttttgagtggctgggatggatcaactctgccaatggcaacacaaaatattctcagaagtttcagggcagagtcgccattaccagggacacatccgcgaggacaacttacatggaattgagcagtctgacatctgaagacacggcgacatattattgtgtgagaggaagaagtccgcccggcgactggttcgacccctggggccagggaaccctggtcaccgtctccgtcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [285, 286] g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a115>c,M39>L(+ + -)|t120>a,H40>Q(- + -)|a131>g,Q44>R(+ - -)|g146>c,R49>T(- - -)|g147>t,R49>T(- - -)|c148>t,L50>F(+ - -)|a157>c,M53>L(+ + -)|g172>t,A58>S(- + -)|g176>c,G59>A(- + -)|t189>c|a204>t|c213>t|a229>g,T77>A(- - -)|c255>g,S85>R(- - -)|g259>a,A87>T(- - -)|c261>t,A87>T(- - -)|g270>a|c271>t|c279>t|g284>c,R95>T(- - -)|t300>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)|c309>t|c314>t,A105>V(+ - +)| g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a115>c,M39>L(+ + -)|t120>a,H40>Q(- + -)|a131>g,Q44>R(+ - -)|g146>c,R49>T(- - -)|g147>t,R49>T(- - -)|c148>t,L50>F(+ - -)|a157>c,M53>L(+ + -)| g172>t,A58>S(- + -)|g176>c,G59>A(- + -)|t189>c| a204>t|c213>t|a229>g,T77>A(- - -)|c255>g,S85>R(- - -)|g259>a,A87>T(- - -)|c261>t,A87>T(- - -)|g270>a|c271>t|c279>t|g284>c,R95>T(- - -)|t300>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)|c309>t| c314>t,A105>V(+ - +)|a320>g| 319 (320) 221 (222) 193 28 (29) 8 (9) 20 2 (3) 5 8 2 2 0 1 1 3 1 2 1 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 51 51 44 7 0 7 1 0 1 0 2 0 0 1 1 0 1 0 30 24 21 3 1 2 0 0 0 1 0 0 0 0 1 0 1 0 117 114 99 15 7 8 1 4 5 1 0 0 1 0 1 1 0 1 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|ta,262-263(FR3)|aa,290-291(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,11-12(FR1)|tt,62-63(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,120-121(FR2)|tt,149-150(FR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,201-202(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcactaattatgct ctgcaatgggtgcgccgggcccccggacaaacttttgagtggctgggatgg atcaactctgccaatggcaacaca aaatattctcagaagtttcagggcagagtcgccattaccagggacacatccgcgaggacaacttacatggaattgagcagtctgacatctgaagacacggcgacatattattgt gtgagaggaagaagtccgcccggcgactggttcgacccc VRGRSPPGDWFDP IGHV1-3 IGHD6-6 IGHJ5 ggatacaccttcactaattatgct ctgcaatgggtgcgccgggcccccggacaaacttttgagtggctgggatgg atcaactctgccaatggcaacaca aaatattctcagaagtttcagggcagagtcgccattaccagggacacatccgcgaggacaacttacatggaattgagcagtctgacatctgaagacacggcgacatattattgt gtgagaggaagaagtccgcccggcgactggttcgacccc unmatched 27 213 16 11 12 18 4 9 7 1 15 4 JY8QFUQ01BANLS 14190 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 804 86.45 185/214 nt NA Homsap IGHJ3*02 F 214 92 46/50 nt Homsap IGHD2-2*01 F 2 8 7 14 8.7.14 [1.17.38.11] CGTSSRAWGADAFDIW in-frame + ctctgggctcagcgtcagtaactaccgcatgggctgggtccgccaggctccagggaaggggctggagtgggtctcagttatttatagagatgatagtacagatcatgtagattccgtgaagggccgattcaccgtttcccgagacaattccaagaacacattgtaccttcagatgaacagtgtgacagccgaggacacggccgtttattattgtggaactagttcccgcgcctggggcgctgatgcctttgatatctggggccaggggacactggtcaccgtctcgtcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccctagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [287] t82>c,F28>L(+ - -)|c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|a109>t,N37>Y(- - -)|t112>c,Y38>R(- - -)|a113>g,Y38>R(- - -)|a118>g,S40>G(+ + -)|c174>a,S58>R(- - -)|g176>a,G59>D(- - -)|g188>a,G63>D(- - -)|c192>t|t196>g,Y66>D(- - -)|c198>t,Y66>D(- - -)|t199>c,Y67>H(+ - -)|c201>t,Y67>H(+ - -)|c203>t,A68>V(+ - +)|c207>t|a232>g,I78>V(+ - +)|c234>t,I78>V(+ - +)|a238>c|g258>a|c259>t|t264>c|a270>g|c279>t|c280>g,L94>V(+ - +)|g284>c,R95>T(- - -)|g303>t|c309>t| t82>c,F28>L(+ - -)|c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|a109>t,N37>Y(- - -)|t112>c,Y38>R(- - -)|a113>g,Y38>R(- - -)| a118>g,S40>G(+ + -)| c174>a,S58>R(- - -)|g176>a,G59>D(- - -)|g188>a,G63>D(- - -)|c192>t| t196>g,Y66>D(- - -)|c198>t,Y66>D(- - -)|t199>c,Y67>H(+ - -)|c201>t,Y67>H(+ - -)|c203>t,A68>V(+ - +)|c207>t|a232>g,I78>V(+ - +)|c234>t,I78>V(+ - +)|a238>c|g258>a|c259>t|t264>c|a270>g|c279>t|c280>g,L94>V(+ - +)|g284>c,R95>T(- - -)|g303>t|c309>t| c314>g,A105>G(- + -)|g315>a,A105>G(- + -)|g317>c,R106>T(- - -)|a318>t,R106>T(- - -)|g319>a|a320>g| 313 (320) 215 (222) 186 (187) 29 (35) 10 (12) 19 (23) 4 (5) 4 (6) 9 4 1 1 1 (2) 0 1 (2) 2 (3) 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 1 1 0 2 0 0 1 0 0 1 0 0 51 51 50 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 30 21 17 4 1 3 0 2 1 0 0 1 0 0 0 0 0 0 117 114 96 18 9 9 2 1 8 2 1 0 0 0 1 1 1 1 1 (8) 1 1 (2) 0 (6) 0 (2) 0 (4) 0 (1) 0 (2) 0 0 0 0 0 (1) 0 0 (1) 0 (1) 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggctcagcgtcagtaactaccgc atgggctgggtccgccaggctccagggaaggggctggagtgggtctcagtt atttatagagatgatagtaca gatcatgtagattccgtgaagggccgattcaccgtttcccgagacaattccaagaacacattgtaccttcagatgaacagtgtgacagccgaggacacggccgtttattattgt ggaactagttcccgcgcctggggcgctgatgcctttgatatc GTSSRAWGADAFDI IGHV3-53 IGHD2-2 IGHJ3 gggctcagcgtcagtaactaccgc atgggctgggtccgccaggctccagggaaggggctggagtgggtctcagtt atttatagagatgatagtaca gatcatgtagattccgtgaagggccgattcaccgtttcccgagacaattccaagaacacattgtaccttcagatgaacagtgtgacagccgaggacacggccgtttattattgt ggaactagttcccgcgcctggggcgctgatgcctttgatatc unmatched 29 210 21 8 13 18 8 11 9 1 10 9 JY8QFUQ01BAR2U 3577 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 873 89.4 194/217 nt NA Homsap IGHJ5*02 F 228 94.12 48/51 nt Homsap IGHD6-6*01 F 2 8 8 14 8.8.14 [1.17.38.11] CARGRATGLVDWFDPW in-frame + ctctggattcaccttcaccaactacgccatgacctgggtccgccaggctccagggaaggggctggagtggatctcgactgttgtgggtggcggtggtaacacatactacgcagactccgtgaagggccggttcaccatctccagagacaattcccagaacacgctgtatttgcaaatgtacaatttgggagccgaggacacggccctatattactgtgcgagaggtagagcgactggtctggtggactggttcgacccctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgagcagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG2 64 88 [291] t90>c|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|t111>c|g119>c,S40>T(+ - +)|g157>a,V53>I(+ - +)|a162>g|g163>a,A55>T(- - -)|a166>g,I56>V(+ - +)|a169>g,S57>V(- - -)|g170>t,S57>V(- - -)|t171>g,S57>V(- - -)|a175>g,S59>G(+ + -)|t177>c,S59>G(+ + -)|g191>a,S64>N(- - -)|a250>c,K84>Q(+ - -)|c265>t|a274>t,N92>Y(- - -)|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|c280>t|a283>g,R95>G(- - -)|g301>c,V101>L(+ - +)|a317>g,K106>R(+ + +)| t90>c|g104>c,S35>T(+ - +)|g107>a,S36>N(- - -)|t111>c| g119>c,S40>T(+ - +)|g157>a,V53>I(+ - +)|a162>g|g163>a,A55>T(- - -)| a166>g,I56>V(+ - +)|a169>g,S57>V(- - -)|g170>t,S57>V(- - -)|t171>g,S57>V(- - -)|a175>g,S59>G(+ + -)|t177>c,S59>G(+ + -)|g191>a,S64>N(- - -)| a250>c,K84>Q(+ - -)|c265>t|a274>t,N92>Y(- - -)|g278>a,S93>N(- - -)|c279>t,S93>N(- - -)|c280>t|a283>g,R95>G(- - -)|g301>c,V101>L(+ - +)| a317>g,K106>R(+ + +)|a320>g| 319 (320) 224 (225) 200 24 (25) 5 (6) 19 6 (7) 5 3 3 1 0 1 0 3 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 1 0 2 0 0 0 0 1 0 0 0 51 51 47 4 1 3 1 2 0 0 0 0 0 0 1 0 0 0 30 24 17 7 0 7 3 1 0 1 0 0 0 0 0 0 1 1 117 114 106 8 2 6 1 1 3 0 1 0 1 0 1 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 1 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcaccaactacgcc atgacctgggtccgccaggctccagggaaggggctggagtggatctcgact gttgtgggtggcggtggtaacaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattcccagaacacgctgtatttgcaaatgtacaatttgggagccgaggacacggccctatattactgt gcgagaggtagagcgactggtctggtggactggttcgacccc ARGRATGLVDWFDP IGHV3-23 IGHD6-6 IGHJ5 ggattcaccttcaccaactacgcc atgacctgggtccgccaggctccagggaaggggctggagtggatctcgact gttgtgggtggcggtggtaacaca tactacgcagactccgtgaagggccggttcaccatctccagagacaattcccagaacacgctgtatttgcaaatgtacaatttgggagccgaggacacggccctatattactgt gcgagaggtagagcgactggtctggtggactggttcgacccc unmatched 23 213 16 7 8 12 8 11 3 2 9 9 JY8QFUQ01BBGFU 16896 productive Homsap IGHV3-7*01 F 941 92.66 202/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-2*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSAFFSTGYW in-frame + gctctagattcacctttaggacatattggatgagttgggtccgccaagctccagggaaggggctggagtgggtggccaacataaaggaagatggaagtgagatacactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtttctccaaatgaacagcttgagagtcgatgacacggctgtgtattactgtgcgatcagtagtgccttcttctccacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [282, 283] c74>g|g79>a,G27>R(- - -)|t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)|c120>t|g132>a|c172>g,Q58>E(+ + -)|a194>t,K65>I(- + -)|t196>c,Y66>H(+ - -)|t256>a,S86>T(+ - +)|a263>t,Y88>F(- + -)|g267>c|c280>t|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)| c74>g| g79>a,G27>R(- - -)|t105>g,S35>R(- - -)|g107>c,S36>T(+ - +)|c108>a,S36>T(+ - +)| c120>t|g132>a| c172>g,Q58>E(+ + -)|a194>t,K65>I(- + -)| t196>c,Y66>H(+ - -)|t256>a,S86>T(+ - +)|a263>t,Y88>F(- + -)|g267>c|c280>t|c287>t,A96>V(+ - +)|g291>t,E97>D(+ - +)| g317>t,R106>I(- + -)|a318>c,R106>I(- + -)|g319>a|a320>g| 316 (320) 222 (226) 206 16 (20) 5 (7) 11 (13) 0 (1) 2 (3) 3 1 0 (1) 1 2 1 2 2 1 (2) 1 78 5 4 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 20 4 0 4 0 1 0 0 0 1 0 0 1 0 0 1 51 51 49 2 2 0 0 1 1 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 117 114 107 7 2 5 0 0 2 1 0 0 1 1 1 0 1 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gctct agattcacctttaggacatattgg atgagttgggtccgccaagctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagata cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtttctccaaatgaacagcttgagagtcgatgacacggctgtgtattactgt gcgatcagtagtgccttcttctccacgggctac AISSAFFSTGY IGHV3-7 IGHD2-2 IGHJ4 agattcacctttaggacatattgg atgagttgggtccgccaagctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagata cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaacacactgtttctccaaatgaacagcttgagagtcgatgacacggctgtgtattactgt gcgatcagtagtgccttcttctccacgggctac unmatched 15 213 6 9 5 10 1 5 4 0 5 6 JY8QFUQ01BCZ6T 18317 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 908 91.63 197/215 nt NA Homsap IGHJ3*02 F 214 92 46/50 nt Homsap IGHD2-21*01 F 2 8 7 15 8.7.15 [1.17.38.11] CTRELRLAAARDGVDIW in-frame + cctctgggttcaccgtcagtagcaagttcatgagctgggtccgccagggtccagggaaggggctggagtgggtctcagttacttatcctgatggtactacacattatagagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgtacgagagaacttaggctggctgctgcgcgtgatggtgttgacatttggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [291, 292] c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)|c134>g,A45>G(- + -)|t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)|t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|t262>g,Y88>D(- - -)|g303>t|c309>t|g313>a,A105>T(- - -)| c111>g,N37>K(+ - -)|a113>t,Y38>F(- + -)| c134>g,A45>G(- + -)| t167>c,I56>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|c174>t,S58>P(+ - -)|g176>a,G59>D(- - -)|g191>c,S64>T(+ - +)|c192>t,S64>T(+ - +)| t196>c,Y66>H(+ - -)|c198>t,Y66>H(+ - -)|c201>t|g202>a,A68>R(- - -)|c203>g,A68>R(- - -)|t262>g,Y88>D(- - -)|g303>t|c309>t| g313>a,A105>T(- - -)| 320 223 204 19 3 16 0 3 5 2 1 0 1 0 2 3 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 0 1 0 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 21 14 7 0 7 0 1 2 1 1 0 0 0 2 0 0 0 117 114 106 8 3 5 0 1 3 1 0 0 0 0 0 1 1 1 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct gggttcaccgtcagtagcaagttc atgagctgggtccgccagggtccagggaaggggctggagtgggtctcagtt acttatcctgatggtactaca cattatagagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttaggctggctgctgcgcgtgatggtgttgacatt TRELRLAAARDGVDI IGHV3-53 IGHD2-21 IGHJ3 gggttcaccgtcagtagcaagttc atgagctgggtccgccagggtccagggaaggggctggagtgggtctcagtt acttatcctgatggtactaca cattatagagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctggatcttcaaatgaacagcctgagagccgaggacacggccgtttattattgt acgagagaacttaggctggctgctgcgcgtgatggtgttgacatt unmatched 18 210 9 9 7 13 2 5 3 0 6 9 JY8QFUQ01BDHSG 18933 productive (see comment) Homsap IGHV1-18*01 F 957 94.39 202/214 nt 93.93 201/214 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD1-26*01 F 1 8 8 12 8.8.12 [0.17.38.11] CATSDVGARKFDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 45 of V-REGION: 'C' (1 nucleotides at position 45 to 45 in the user submitted sequence), (cause frameshift); tggttacacctttaccagatatggtattagttgggtgcgacaggCcccctggacaagggcttgagtggatgggatggatcagcgtttccaatggtgacacaaactatgcacagaagctccagggcagagtcaccatgaccgcagacccatccacgagcacagcctacttggaactgaggagcctgacatctgacgacacggccgtatattactgtgcgacatctgacgttggagctcgaaagtttgactactggggccagggaacccaggtcaccgtctcctcagcatcccccgaccagccccaaggtcttcccgctgagcctccgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 29 74 [283, 285] c108>a,S36>R(- - -)|c117>t|c120>t|c173>t,A58>V(+ - +)|a176>c,Y59>S(+ - -)|a190>g,N64>D(+ + -)|a238>g,T80>A(- - -)|a244>c,T82>P(+ + -)|a265>t,M89>L(+ + -)|g270>a|g284>c,R95>T(- - -)|g303>a| c108>a,S36>R(- - -)| c117>t|c120>t| c173>t,A58>V(+ - +)|a176>c,Y59>S(+ - -)|a190>g,N64>D(+ + -)| a238>g,T80>A(- - -)|a244>c,T82>P(+ + -)|a265>t,M89>L(+ + -)|g270>a|g284>c,R95>T(- - -)|g303>a| g317>c,R106>T(- - -)|g319>t|a320>c| 316 (320) 218 (222) 206 (207) 12 (15) 4 (6) 8 (9) 2 2 3 0 2 (3) 1 1 0 1 (2) 0 0 (1) 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 49 2 2 0 0 0 2 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 1 0 1 0 0 0 0 0 0 0 117 114 108 6 2 4 1 2 0 0 1 0 1 0 1 0 0 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 (1) 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|ta,175-176(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,5-6(FR1)|tt,75-76(FR1)|tt,81-82(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|tt,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,3-6(FR1)|agct,8-11(FR1)|agct,24-27(FR1)|ggct,72-75(FR1)|ggtt,79-82(CDR1)|agct,106-109(CDR1)|ggta,112-115(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)| agct,8-11(FR1)|agct,24-27(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|agct,209-212(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggttacacctttaccagatatggt attagttgggtgcgacaggcccctggacaagggcttgagtggatgggatgg atcagcgtttccaatggtgacaca aactatgcacagaagctccagggcagagtcaccatgaccgcagacccatccacgagcacagcctacttggaactgaggagcctgacatctgacgacacggccgtatattactgt gcgacatctgacgttggagctcgaaagtttgactac ATSDVGARKFDY IGHV1-18 IGHD1-26 IGHJ4 ggttacacctttaccagatatggt attagttgggtgcgacaggcccctggacaagggcttgagtggatgggatgg atcagcgtttccaatggtgacaca aactatgcacagaagctccagggcagagtcaccatgaccgcagacccatccacgagcacagcctacttggaactgaggagcctgacatctgacgacacggccgtatattactgt gcgacatctgacgttggagctcgaaagtttgactac unmatched 12 213 7 5 5 7 2 5 4 0 4 4 JY8QFUQ01BF6IL 10129 productive Homsap IGHV3-7*01 F 990 95.39 207/217 nt NA Homsap IGHJ4*02 F 87 64.58 31/48 nt Homsap IGHD2-15*01 F 2 8 8 12 8.8.12 [1.17.38.11] CAKGGGNGDLDYWG in-frame + ctctggattcacctttagtaaccattggatgaactgggtccgccaggctccagggaaggggctggagtgggtggccaacataatgccagatggaggtgagaaattctatgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgaaggggggtggtaacggcgaccttgactactgggggccaggggaacccctggtcaccgtctcctcagcatcccccgaccagccccaaggttcttgcccgctgagcctcgacagnaccccccaagatgggaacgtggtccgtcgcatgcctggtccagggcttcttcccc NA unmatched, IGA2 79 63 [287, 285] g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)|g119>a,S40>N(- - -)|a170>t,K57>M(- + -)|a173>c,Q58>P(- - -)|a187>g,S63>G(+ + -)|a197>t,Y66>F(- + -)|a225>g|a245>g,N82>S(- - -)|c265>t| g107>a,S36>N(- - -)|t109>c,Y37>H(+ - -)| g119>a,S40>N(- - -)| a170>t,K57>M(- + -)|a173>c,Q58>P(- - -)|a187>g,S63>G(+ + -)| a197>t,Y66>F(- + -)|a225>g|a245>g,N82>S(- - -)|c265>t| g317>a,R106>K(+ + +)|a318>g,R106>K(+ + +)|a320>g| 316 (320) 221 (225) 211 (212) 10 (13) 2 (3) 8 (10) 3 (5) 2 (3) 1 1 1 0 2 0 0 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 0 0 1 0 1 0 0 0 0 0 117 114 110 4 2 2 2 0 1 0 0 0 1 0 0 0 0 0 4 (8) 4 4 (5) 0 (3) 0 (1) 0 (2) 0 (2) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtaaccattgg atgaactgggtccgccaggctccagggaaggggctggagtgggtggccaac ataatgccagatggaggtgagaaa ttctatgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgaaggggggtggtaacggcgaccttgactactgg AKGGGNGDLDYW IGHV3-7 IGHD2-15 IGHJ4 ggattcacctttagtaaccattgg atgaactgggtccgccaggctccagggaaggggctggagtgggtggccaac ataatgccagatggaggtgagaaa ttctatgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt gcgaaggggggtggtaacggcgaccttgactactgg unmatched 10 213 7 3 3 3 4 7 2 0 3 5 JY8QFUQ01BG2KZ 11596 productive Homsap IGHV3-7*01 F 932 92.2 201/218 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [1.17.38.11] CVRGRDGVNFGEMDYW in-frame + cctctgggttcatctttagtagacattggatggcctgggtccgccaggctccagggaagaggctggagtgggtggccaacataaaacaagatggaagtctgagatactttgtggactctgtgaagggccgattcaccatctccagagacaacgccgagagctcactgtttctgcaaatggacagcctgagaggcgaggacacggctgtgtattactgtgtgaggggtagagatggtgtcaattttggcgaaatggactactggggccagggaatcctggtcagcgtctcctcggcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaaaggatgggaacgtggtcgtcgcatgctggtcccaggggcttcttccccc NA unmatched, IGA2 64 84 [291, 294] a81>g|c86>t,T29>I(- - -)|c108>a,S36>R(- - -)|t109>c,Y37>H(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g145>a,G49>R(- - -)|g171>a|g190>c,E64>L(- - -)|a191>t,E64>L(- - -)|a194>g,K65>R(+ + +)|a200>t,Y67>F(- + -)|a250>g,K84>E(+ - -)|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c287>g,A96>G(- + -)|c314>t,A105>V(+ - +)| a81>g|c86>t,T29>I(- - -)|c108>a,S36>R(- - -)|t109>c,Y37>H(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|g145>a,G49>R(- - -)| g171>a|g190>c,E64>L(- - -)|a191>t,E64>L(- - -)|a194>g,K65>R(+ + +)| a200>t,Y67>F(- + -)|a250>g,K84>E(+ - -)|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c287>g,A96>G(- + -)| c314>t,A105>V(+ - +)|a318>g|a320>g| 317 (320) 223 (226) 205 (206) 18 (20) 2 (4) 16 6 (8) 2 2 1 0 1 3 0 2 1 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 0 1 1 0 1 0 0 0 0 0 0 51 51 48 3 0 3 1 1 0 0 0 0 0 0 1 0 0 0 30 24 20 4 1 3 1 1 0 0 0 0 1 0 1 0 0 0 117 114 108 6 0 6 3 0 0 0 0 0 2 0 0 1 0 0 5 (8) 5 4 (5) 1 (3) 0 (2) 1 0 (2) 0 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct gggttcatctttagtagacattgg atggcctgggtccgccaggctccagggaagaggctggagtgggtggccaac ataaaacaagatggaagtctgaga tactttgtggactctgtgaagggccgattcaccatctccagagacaacgccgagagctcactgtttctgcaaatggacagcctgagaggcgaggacacggctgtgtattactgt gtgaggggtagagatggtgtcaattttggcgaaatggactac VRGRDGVNFGEMDY IGHV3-7 IGHD5-24 IGHJ4 gggttcatctttagtagacattgg atggcctgggtccgccaggctccagggaagaggctggagtgggtggccaac ataaaacaagatggaagtctgaga tactttgtggactctgtgaagggccgattcaccatctccagagacaacgccgagagctcactgtttctgcaaatggacagcctgagaggcgaggacacggctgtgtattactgt gtgaggggtagagatggtgtcaattttggcgaaatggactac unmatched 17 213 10 7 3 7 7 10 0 2 9 6 JY8QFUQ01BG9LH 18701 productive Homsap IGHV3-7*01 F 914 91.28 199/218 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD4-17*01 F 3 8 8 14 8.8.14 [1.17.38.11] CATSPFTVTTGKDAYW in-frame + cctctagattcacctttaatggttactggatgagttgggtccgccaggctccaggaaaggggctggagtggctggccaacataaagccggatggaaatgagaaatgctatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagagttcgctgtttctgcaaatgaacagcctgagagccgaggacacggctgtatatttctgtgcgacctccccctttacggtgactactgggaaggacgcctactggggccagggaaccctggtcctcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccaagatgggaacgtggtcgtcccatgctggtccaggggcttcttccccctag NA unmatched, IGA2 64 84 [291, 292] g79>a,G27>R(- - -)|g104>a,S35>N(- - -)|a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)|t111>c|c120>t|g141>a|g157>c,V53>L(+ - +)|a173>c,Q58>P(- - -)|a174>g,Q58>P(- - -)|g188>a,S63>N(- - -)|a197>g,Y66>C(- - -)|t203>c,V68>A(+ - +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a258>g|a263>t,Y88>F(- + -)|g303>a|a308>t,Y103>F(- + -)| g79>a,G27>R(- - -)|g104>a,S35>N(- - -)|a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)|t111>c| c120>t|g141>a|g157>c,V53>L(+ - +)| a173>c,Q58>P(- - -)|a174>g,Q58>P(- - -)|g188>a,S63>N(- - -)| a197>g,Y66>C(- - -)|t203>c,V68>A(+ - +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a258>g|a263>t,Y88>F(- + -)|g303>a|a308>t,Y103>F(- + -)| g317>c,R106>T(- - -)|a318>c,R106>T(- - -)|g319>t|a320>c| 316 (320) 222 (226) 203 19 (23) 5 (7) 14 (16) 5 5 3 2 1 (3) 0 2 0 1 (2) 0 0 (1) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 1 2 1 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 1 1 0 0 0 0 0 1 0 0 0 30 24 21 3 0 3 1 1 0 0 1 0 0 0 0 0 0 0 117 114 106 8 2 6 3 1 1 1 0 0 2 0 0 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 0 0 0 0 (2) 0 0 0 0 (1) 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct agattcacctttaatggttactgg atgagttgggtccgccaggctccaggaaaggggctggagtggctggccaac ataaagccggatggaaatgagaaa tgctatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagagttcgctgtttctgcaaatgaacagcctgagagccgaggacacggctgtatatttctgt gcgacctccccctttacggtgactactgggaaggacgcctac ATSPFTVTTGKDAY IGHV3-7 IGHD4-17 IGHJ4 agattcacctttaatggttactgg atgagttgggtccgccaggctccaggaaaggggctggagtggctggccaac ataaagccggatggaaatgagaaa tgctatgcggactctgtgaagggccgattcaccatctccagagacaacgccaagagttcgctgtttctgcaaatgaacagcctgagagccgaggacacggctgtatatttctgt gcgacctccccctttacggtgactactgggaaggacgcctac unmatched 19 213 15 4 8 9 7 10 4 1 7 7 JY8QFUQ01BGU0C 9570 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 850 88.43 191/216 nt NA Homsap IGHJ6*02 F (see comment) 202 80.65 50/62 nt Homsap IGHD2-21*02 F 3 8 7 16 8.7.16 [2.17.38.11] CAREFRAPSLARYGMDVW in-frame + Other possibilities: Homsap_IGHJ6*01 and Homsap_IGHJ6*04 (highest number of consecutive identical nucleotides) cctcgggggttcagcgtcagtaataacttcatgacctgggtccgccaggttccagggaaggggctggagtgggtctcagttatttatagcaatggtgaaacaatctacgcagactccgtgaagggccgattcactatgtccagagacaattccaagaacacactgtttcttcaaatgaacagcctgagaggcgaggacacggccgtgtaccactgtgcgagagaatttcgggcgccttctttggccagatacggtatggatgtctggggccacgggaccacggtcaccgtctcctcagcatcccctgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 79 [296, 297] g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>R(- - -)|c77>g,S26>R(- - -)|t78>g,S26>R(- - -)|c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a113>t,Y38>F(- + -)|g119>c,S40>T(+ - +)|c134>t,A45>V(+ - +)|g175>a,G59>N(- - -)|g176>a,G59>N(- - -)|a190>g,S64>E(- - -)|g191>a,S64>E(- - -)|c192>a,S64>E(- - -)|t196>a,Y66>I(- - -)|a197>t,Y66>I(- - -)|c231>t|c234>g,I78>M(+ + -)|g258>a|a263>t,Y88>F(- + -)|c287>g,A96>G(- + -)|t306>c|t307>c,Y103>H(+ - -)| g73>c,A25>P(- - -)|c75>t,A25>P(- - -)|t76>c,S26>R(- - -)|c77>g,S26>R(- - -)|t78>g,S26>R(- - -)| c86>g,T29>S(+ - +)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a113>t,Y38>F(- + -)| g119>c,S40>T(+ - +)|c134>t,A45>V(+ - +)| g175>a,G59>N(- - -)|g176>a,G59>N(- - -)|a190>g,S64>E(- - -)|g191>a,S64>E(- - -)|c192>a,S64>E(- - -)| t196>a,Y66>I(- - -)|a197>t,Y66>I(- - -)|c231>t|c234>g,I78>M(+ + -)|g258>a|a263>t,Y88>F(- + -)|c287>g,A96>G(- + -)|t306>c|t307>c,Y103>H(+ - -)| 320 224 199 25 3 22 1 5 4 3 0 1 3 1 2 4 0 1 78 6 1 5 0 5 0 0 1 1 0 0 0 0 1 1 0 1 36 24 20 4 0 4 0 1 1 0 0 0 1 0 0 1 0 0 51 51 49 2 0 2 0 0 1 0 0 0 0 0 1 0 0 0 30 21 16 5 0 5 1 3 0 0 0 1 0 0 0 0 0 0 117 114 105 9 3 6 0 1 1 2 0 0 2 1 0 2 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctcgg gggttcagcgtcagtaataacttc atgacctgggtccgccaggttccagggaaggggctggagtgggtctcagtt atttatagcaatggtgaaaca atctacgcagactccgtgaagggccgattcactatgtccagagacaattccaagaacacactgtttcttcaaatgaacagcctgagaggcgaggacacggccgtgtaccactgt gcgagagaatttcgggcgccttctttggccagatacggtatggatgtc AREFRAPSLARYGMDV IGHV3-53 IGHD2-21 IGHJ6 gggttcagcgtcagtaataacttc atgacctgggtccgccaggttccagggaaggggctggagtgggtctcagtt atttatagcaatggtgaaaca atctacgcagactccgtgaagggccgattcactatgtccagagacaattccaagaacacactgtttcttcaaatgaacagcctgagaggcgaggacacggccgtgtaccactgt gcgagagaatttcgggcgccttctttggccagatacggtatggatgtc unmatched 20 210 11 9 8 13 3 7 3 0 8 9 JY8QFUQ01BHYPA 15369 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 1004 95.87 209/218 nt NA Homsap IGHJ4*02 F 240 100 48/48 nt Homsap IGHD3-10*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKKYHYGSGTYFYYFDYW in-frame + cctctggattcacctttagccactatgccgtgacctgggtccgccaggctccagggaagggtctggagtgggtctcaactattagtggtagtgatggtagcacgtactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgaagaagtatcactatggttcggggacttacttctactactttgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagcccccaaggttcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA unmatched, IGA2 57 74 [303, 302, 300] a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)|a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|g147>t|g163>a,A55>T(- - -)|g185>a,G62>D(- - -)|a195>g|a215>g,K72>R(+ + +)| a106>c,S36>H(+ - -)|g107>a,S36>H(+ - -)| a115>g,M39>V(+ - -)|g119>c,S40>T(+ - +)|g147>t|g163>a,A55>T(- - -)| g185>a,G62>D(- - -)|a195>g| a215>g,K72>R(+ + +)| a318>g|g319>a| 317 (320) 223 (226) 214 (215) 9 (11) 2 (4) 7 3 (4) 3 (4) 0 0 1 0 0 0 1 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 0 1 0 0 0 0 0 0 0 51 51 47 4 1 3 1 1 0 0 0 0 0 0 1 0 1 0 30 24 22 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 117 114 113 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagccactatgcc gtgacctgggtccgccaggctccagggaagggtctggagtgggtctcaact attagtggtagtgatggtagcacg tactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaagaagtatcactatggttcggggacttacttctactactttgactac AKKYHYGSGTYFYYFDY IGHV3-23 IGHD3-10 IGHJ4 ggattcacctttagccactatgcc gtgacctgggtccgccaggctccagggaagggtctggagtgggtctcaact attagtggtagtgatggtagcacg tactacgcagactccgtgaggggccggttcaccatctccagagacaattccaagaacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgaagaagtatcactatggttcggggacttacttctactactttgactac unmatched 9 213 6 3 3 5 3 4 1 1 4 3 JY8QFUQ01BJC1Y 9517 productive Homsap IGHV3-7*01 F 1008 96.31 209/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSGYYSTGYW in-frame + ctctggattcagctttagttactattggatgagctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaaggaagatggaagtgagagacactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgtgcgattagtagtggttattactcgacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcctcccgctgagcctccgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 29 68 [283, 281, 273] c86>g,T29>S(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)|t196>c,Y66>H(+ - -)|a254>g,N85>S(- - -)|g303>a| c86>g,T29>S(+ - +)|a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)| c172>g,Q58>E(+ + -)|a194>g,K65>R(+ + +)| t196>c,Y66>H(+ - -)|a254>g,N85>S(- - -)|g303>a| g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 316 (320) 221 (225) 213 8 (12) 1 (3) 7 (9) 2 (3) 2 (3) 0 1 0 0 1 (2) 0 0 2 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 1 0 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 1 0 0 117 114 111 3 1 2 1 1 0 1 0 0 0 0 0 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagctttagttactattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgattagtagtggttattactcgacgggctac AISSGYYSTGY IGHV3-7 IGHD3-22 IGHJ4 ggattcagctttagttactattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaggaagatggaagtgagaga cactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagagctcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtatattactgt gcgattagtagtggttattactcgacgggctac unmatched 8 213 5 3 2 4 3 4 1 0 2 5 JY8QFUQ01BKLR9 21246 productive (see comment) Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 896 90.37 197/218 nt 89.91 196/218 nt Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD4-17*01 F 2 8 8 20 8.8.20 [1.17.38.11] CTRTQFDPDYGDYETGYGMDVW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 49 of V-REGION: 'G' (1 nucleotides at position 61 to 61 in the user submitted sequence), (cause frameshift); cggctggattcaacttccgatcttatgccatgtactgggtccgccaggccccaggcaaggGggctggactgggtggcagttatttggcatgatggcagtaatcaatactatgcagattccgtgaagggccgattcaccatctccagagacaattccaagaacacattgtttctgcaaatgaacagcctgagagtcgaggacacggctgtctattactgtacgaggacgcaatttgaccctgactacggtgactacgagacggggtacggtatggacgtctggggccaagggacaacggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgccatggcctggtccagggcttcttccc NA unmatched, IGA2 36 79 [310, 311] t76>g,S26>A(- + -)|c86>a,T29>N(- + -)|a103>c,S35>R(- - -)|t105>a,S35>R(- - -)|a106>t|g107>c|c108>t|g113>c,G38>A(- + -)|c118>t,H40>Y(+ - -)|t135>c|g153>c,E51>D(+ - +)|a168>t|t172>c,Y58>H(+ - -)|a186>c|a193>c,K65>Q(+ - -)|c207>t|g258>a|c259>t|a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|g303>c|g313>a,A105>T(- - -)| t76>g,S26>A(- + -)| c86>a,T29>N(- + -)|a103>c,S35>R(- - -)|t105>a,S35>R(- - -)|a106>t|g107>c|c108>t|g113>c,G38>A(- + -)| c118>t,H40>Y(+ - -)|t135>c|g153>c,E51>D(+ - +)| a168>t|t172>c,Y58>H(+ - -)|a186>c|a193>c,K65>Q(+ - -)| c207>t|g258>a|c259>t|a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|g303>c| g313>a,A105>T(- - -)|a318>g|g319>a|a320>c| 317 (320) 223 (226) 201 22 (25) 10 (13) 12 0 (1) 2 (3) 5 2 3 (4) 1 3 1 4 0 0 1 78 5 4 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 36 24 17 7 3 4 0 0 1 0 1 1 1 1 2 0 0 0 51 51 48 3 1 2 0 0 1 1 0 0 0 0 1 0 0 0 30 24 20 4 2 2 0 0 0 1 2 0 1 0 0 0 0 0 117 114 108 6 4 2 0 1 3 0 0 0 1 0 1 0 0 0 5 (8) 5 4 1 (4) 0 (3) 1 0 (1) 1 (2) 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cggct ggattcaacttccgatcttatgcc atgtactgggtccgccaggccccaggcaaggggctggactgggtggcagtt atttggcatgatggcagtaatcaa tactatgcagattccgtgaagggccgattcaccatctccagagacaattccaagaacacattgtttctgcaaatgaacagcctgagagtcgaggacacggctgtctattactgt acgaggacgcaatttgaccctgactacggtgactacgagacggggtacggtatggacgtc TRTQFDPDYGDYETGYGMDV IGHV3-33 IGHD4-17 IGHJ6 ggattcaacttccgatcttatgcc atgtactgggtccgccaggccccaggcaaggggctggactgggtggcagtt atttggcatgatggcagtaatcaa tactatgcagattccgtgaagggccgattcaccatctccagagacaattccaagaacacattgtttctgcaaatgaacagcctgagagtcgaggacacggctgtctattactgt acgaggacgcaatttgaccctgactacggtgactacgagacggggtacggtatggacgtc unmatched 20 213 8 12 6 11 2 9 5 5 4 6 JY8QFUQ01BLF36 9065 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 733 82.41 178/216 nt NA Homsap IGHJ3*01 F 83 62 31/50 nt Homsap IGHD5-24*01 ORF 3 8 8 14 8.8.14 [1.17.38.11] CVKDKAGFTLHAFDVX in-frame + Low V-REGION identity (82,41% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release tctggattcgactttgccagccacgccatggcttgggtccgccggactccaaggaagggcctggagtgggtctcaggcataagtagtagtggtggaaccacgtattacgcagacttcgtgaagggccgcgccactgtctccagagacaattccgagaacacagtgtctctggaactccacagcctgagagccgatgacacggccatatattattgtgtgaaggacaaagcgggcttcaccctccatgcttttgatgtcnggggccgagggacggtgtcactgtttcttcaacatccccgaccagccccaaggctcttcccgctgagcctctgcagcaccctagccagatgggaacgctggtcgatcgccctgcctggnccagggcttcttcccccatgga NA unmatched, IGA1 57 63 [289, 288] a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c120>t,S40>A(- + -)|a131>g,Q44>R(+ - -)|g133>a,A45>T(- - -)|g139>a,G47>R(- - -)|g147>c|c164>g,A55>G(- + -)|t165>c,A55>G(- + -)|t168>a|g172>a,G58>S(+ + -)|t189>a|g191>c,S64>T(+ - +)|a195>g|c198>t|c209>t,S70>F(- - -)|g225>c|t226>g,F76>A(+ - -)|t227>c,F76>A(+ - -)|c231>t|a232>g,I78>V(+ - +)|a250>g,K84>E(+ - -)|g258>a|c259>g,L87>V(+ - +)|a263>c,Y88>S(+ - -)|c268>g,Q90>E(+ + -)|a271>c,M91>L(+ + -)|g273>c,M91>L(+ + -)|a274>c,N92>H(- - -)|g291>t,E97>D(+ - +)|g301>a,V101>I(+ - +)|c309>t|c314>t,A105>V(+ - +)|a318>g| a85>g,T29>D(- + -)|c86>a,T29>D(- + -)|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|t109>c,Y37>H(+ - -)|t111>c,Y37>H(+ - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c120>t,S40>A(- + -)|a131>g,Q44>R(+ - -)|g133>a,A45>T(- - -)|g139>a,G47>R(- - -)|g147>c|c164>g,A55>G(- + -)|t165>c,A55>G(- + -)| t168>a|g172>a,G58>S(+ + -)|t189>a|g191>c,S64>T(+ - +)|a195>g| c198>t|c209>t,S70>F(- - -)|g225>c|t226>g,F76>A(+ - -)|t227>c,F76>A(+ - -)|c231>t|a232>g,I78>V(+ - +)|a250>g,K84>E(+ - -)|g258>a|c259>g,L87>V(+ - +)|a263>c,Y88>S(+ - -)|c268>g,Q90>E(+ + -)|a271>c,M91>L(+ + -)|g273>c,M91>L(+ + -)|a274>c,N92>H(- - -)|g291>t,E97>D(+ - +)|g301>a,V101>I(+ - +)|c309>t| c314>t,A105>V(+ - +)|a318>g| 320 224 184 40 10 30 8 5 6 4 3 1 0 2 6 3 1 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 2 0 0 2 0 1 0 0 1 0 0 0 51 51 42 9 1 8 2 2 1 1 0 0 0 0 2 1 0 0 30 24 19 5 3 2 1 1 0 0 0 0 0 2 1 0 0 0 117 114 96 18 5 13 2 2 4 1 3 0 0 0 2 2 1 1 8 8 6 2 1 1 1 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcgactttgccagccacgcc atggcttgggtccgccggactccaaggaagggcctggagtgggtctcaggc ataagtagtagtggtggaaccacg tattacgcagacttcgtgaagggccgcgccactgtctccagagacaattccgagaacacagtgtctctggaactccacagcctgagagccgatgacacggccatatattattgt gtgaaggacaaagcgggcttcaccctccatgcttttgatgtc VKDKAGFTLHAFDV IGHV3-23 IGHD5-24 IGHJ3 ggattcgactttgccagccacgcc atggcttgggtccgccggactccaaggaagggcctggagtgggtctcaggc ataagtagtagtggtggaaccacg tattacgcagacttcgtgaagggccgcgccactgtctccagagacaattccgagaacacagtgtctctggaactccacagcctgagagccgatgacacggccatatattattgt gtgaaggacaaagcgggcttcaccctccatgcttttgatgtc unmatched 38 213 21 17 10 21 11 17 6 3 21 8 JY8QFUQ01BLJYE 12627 productive (see comment) Homsap IGHV3-11*01 F (see comment) 765 83.87 182/217 nt 83.41 181/217 nt Homsap IGHJ3*01 F, or Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD2-15*01 F 2 8 8 15 8.8.15 [1.17.38.11] CARVRCSGNTCGAFDLW in-frame + Potentially, because of detected insertion/deletion Low V-REGION identity (83,87% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR3-IMGT at codon 88 of V-REGION: 'T' (1 nucleotides at position 167 to 167 in the user submitted sequence), (cause frameshift); ctctggattcagcttaagtgactactacatgacctgggtccgccaggccccagggaagggactggagtggctcgcctacattagtcgaactgatgattccgtatattccgcagagtctgtggtgggccgattcaccgtctccagggacaacgtccaaaactcactgTtttttgcagatgattggcctgagagacgaggacacggccgtatattactgtgcgagagtccgctgtagtggtaatacctgcggggcttttgatttgtggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [294, 295] c86>g,T29>S(+ - +)|c90>a,F30>L(+ - -)|g119>c,S40>T(+ - +)|a124>g,I42>V(+ - +)|t135>c|g147>a|g157>c,V53>L(+ - +)|t159>c,V53>L(+ - +)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)|a172>c,S58>R(- - -)|t174>a,S58>R(- - -)|g176>c,S59>T(+ - +)|g185>a,G62>D(- - -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a190>t,T64>S(+ - +)|a193>g,I65>V(+ - +)|c198>t|a200>c,Y67>S(+ - -)|c207>g,D69>E(+ - +)|a214>g,K72>V(- - -)|a215>t,K72>V(- - -)|a232>g,I78>V(+ - +)|c248>t,A83>V(+ - +)|a250>c,K84>Q(+ - -)|g252>a,K84>Q(+ - -)|a263>t,Y88>F(- + -)|c265>t|a270>g|a275>t,N92>I(- - -)|c276>t,N92>I(- - -)|a277>g,S93>G(+ + -)|c287>a,A96>D(- - -)|g303>a| c86>g,T29>S(+ - +)|c90>a,F30>L(+ - -)| g119>c,S40>T(+ - +)|a124>g,I42>V(+ - +)|t135>c|g147>a|g157>c,V53>L(+ - +)|t159>c,V53>L(+ - +)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)| a172>c,S58>R(- - -)|t174>a,S58>R(- - -)|g176>c,S59>T(+ - +)|g185>a,G62>D(- - -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a190>t,T64>S(+ - +)|a193>g,I65>V(+ - +)| c198>t|a200>c,Y67>S(+ - -)|c207>g,D69>E(+ - +)|a214>g,K72>V(- - -)|a215>t,K72>V(- - -)|a232>g,I78>V(+ - +)|c248>t,A83>V(+ - +)|a250>c,K84>Q(+ - -)|g252>a,K84>Q(+ - -)|a263>t,Y88>F(- + -)|c265>t|a270>g|a275>t,N92>I(- - -)|c276>t,N92>I(- - -)|a277>g,S93>G(+ + -)|c287>a,A96>D(- - -)|g303>a| a320>t| 319 (320) 224 (225) 189 35 (36) 6 (7) 29 7 5 4 2 4 2 4 (5) 1 3 2 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 1 0 0 0 1 0 0 51 51 43 8 2 6 1 1 0 2 1 0 0 0 2 0 0 1 30 24 16 8 0 8 2 2 0 0 1 0 1 1 1 0 0 0 117 114 97 17 4 13 4 2 4 0 2 1 3 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagcttaagtgactactac atgacctgggtccgccaggccccagggaagggactggagtggctcgcctac attagtcgaactgatgattccgta tattccgcagagtctgtggtgggccgattcaccgtctccagggacaacgtccaaaactcactgtttttgcagatgattggcctgagagacgaggacacggccgtatattactgt gcgagagtccgctgtagtggtaatacctgcggggcttttgatttg ARVRCSGNTCGAFDL IGHV3-11 IGHD2-15 IGHJ3 ggattcagcttaagtgactactac atgacctgggtccgccaggccccagggaagggactggagtggctcgcctac attagtcgaactgatgattccgta tattccgcagagtctgtggtgggccgattcaccgtctccagggacaacgtccaaaactcactgtttttgcagatgattggcctgagagacgaggacacggccgtatattactgt gcgagagtccgctgtagtggtaatacctgcggggcttttgatttg unmatched 35 213 18 17 9 16 9 19 6 0 19 10 JY8QFUQ01BLLRQ 7222 productive Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 977 94.5 206/218 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD2-8*01 F 2 8 8 16 8.8.16 [1.17.38.11] CARDRGCGNGACHVADYW in-frame + cctctggattcaccttcagtaactatagtatgaactgggtccgccaggctccagggaagggcctggagtgggtctcatccatcagtagtggtggtagtttcaaacaccacgcagactcagcgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattattgtgcgagagatcggggttgtggtaatggtgcatgccatgtcgccgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccag NA unmatched, IGA2 57 89 [297, 298] g107>a,S36>N(- - -)|c114>t|g147>c|t168>c|a175>g,S59>G(+ + -)|a184>g,S62>G(+ + -)|a191>t,Y64>F(- + -)|t194>a,I65>K(- + -)|t196>c,Y66>H(+ - -)|t199>c,Y67>H(+ - -)|t212>c,V71>A(+ - +)|c309>t| g107>a,S36>N(- - -)|c114>t| g147>c| t168>c|a175>g,S59>G(+ + -)|a184>g,S62>G(+ + -)|a191>t,Y64>F(- + -)|t194>a,I65>K(- + -)| t196>c,Y66>H(+ - -)|t199>c,Y67>H(+ - -)|t212>c,V71>A(+ - +)|c309>t| 320 226 214 12 4 8 2 1 2 4 0 0 1 1 1 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 19 5 1 4 2 0 0 1 0 0 1 1 0 0 0 0 117 114 110 4 1 3 0 0 1 3 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtaactatagt atgaactgggtccgccaggctccagggaagggcctggagtgggtctcatcc atcagtagtggtggtagtttcaaa caccacgcagactcagcgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattattgt gcgagagatcggggttgtggtaatggtgcatgccatgtcgccgactac ARDRGCGNGACHVADY IGHV3-21 IGHD2-8 IGHJ4 ggattcaccttcagtaactatagt atgaactgggtccgccaggctccagggaagggcctggagtgggtctcatcc atcagtagtggtggtagtttcaaa caccacgcagactcagcgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattattgt gcgagagatcggggttgtggtaatggtgcatgccatgtcgccgactac unmatched 12 213 9 3 3 4 6 8 2 2 3 5 JY8QFUQ01BM2SX 19631 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 851 88.07 192/218 nt NA Homsap IGHJ6*01 F (see comment) 171 74.6 47/63 nt Homsap IGHD2-2*01 F 1 8 8 13 8.8.13 [1.17.38.11] CVSDRELHYQHLDVW in-frame + Other possibilities: Homsap_IGHJ3*01 (shorter alignment but highest percentage of identity) cgtctggattcaacttcaatagttttggcatgcactgggtccgccaggctccgggcaagggactggagtgggtggcaaacatatggtatgatggaggtagtcaacactatgcagacctcgtgaagggccgattcaccatctctagagacaattccaagaacatcttgttcctgcaaatgagcgacctgagagccgaggacacggctgtttattattgtgtgagcgatagagaactccattaccagcatttggacgtctggggtcaagggaccacggtcatcgtctcatcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG1 64 88 [289] c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|c108>t|a110>t,Y37>F(- + -)|a138>g|g147>a|g163>a,V55>N(- - -)|t164>a,V55>N(- - -)|t165>c,V55>N(- - -)|a187>g,S63>G(+ + -)|a191>g,N64>S(- - -)|a193>c,K65>Q(+ - -)|t196>c,Y66>H(+ - -)|t208>c,S70>L(- - -)|c209>t,S70>L(- - -)|c237>t|c257>t,T86>I(- - -)|g258>c,T86>I(- - -)|c259>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|a275>g,N92>S(- - -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g303>t|c309>t|c314>t,A105>V(+ - +)|a318>c,R106>S(- - -)| c86>a,T29>N(- + -)|g104>a,S35>N(- - -)|c108>t|a110>t,Y37>F(- + -)| a138>g|g147>a|g163>a,V55>N(- - -)|t164>a,V55>N(- - -)|t165>c,V55>N(- - -)| a187>g,S63>G(+ + -)|a191>g,N64>S(- - -)|a193>c,K65>Q(+ - -)| t196>c,Y66>H(+ - -)|t208>c,S70>L(- - -)|c209>t,S70>L(- - -)|c237>t|c257>t,T86>I(- - -)|g258>c,T86>I(- - -)|c259>t|a263>t,Y88>F(- + -)|t264>c,Y88>F(- + -)|a275>g,N92>S(- - -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g303>t|c309>t| c314>t,A105>V(+ - +)|a318>c,R106>S(- - -)| 320 226 198 28 7 21 5 4 7 4 2 1 2 1 1 0 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 1 0 0 1 1 0 0 0 0 0 51 51 46 5 2 3 1 2 0 1 0 0 0 1 0 0 0 0 30 24 21 3 0 3 2 0 0 0 1 0 0 0 0 0 0 0 117 114 100 14 4 10 2 1 5 3 0 0 1 0 1 0 1 0 8 8 6 2 0 2 0 0 1 0 1 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cgtct ggattcaacttcaatagttttggc atgcactgggtccgccaggctccgggcaagggactggagtgggtggcaaac atatggtatgatggaggtagtcaa cactatgcagacctcgtgaagggccgattcaccatctctagagacaattccaagaacatcttgttcctgcaaatgagcgacctgagagccgaggacacggctgtttattattgt gtgagcgatagagaactccattaccagcatttggacgtc VSDRELHYQHLDV IGHV3-33 IGHD2-2 IGHJ6 ggattcaacttcaatagttttggc atgcactgggtccgccaggctccgggcaagggactggagtgggtggcaaac atatggtatgatggaggtagtcaa cactatgcagacctcgtgaagggccgattcaccatctctagagacaattccaagaacatcttgttcctgcaaatgagcgacctgagagccgaggacacggctgtttattattgt gtgagcgatagagaactccattaccagcatttggacgtc unmatched 26 213 19 7 10 13 9 13 6 1 13 6 JY8QFUQ01BM631 13315 productive Homsap IGHV3-11*01 F 995 95.41 208/218 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD1-26*01 F 3 8 8 14 8.8.14 [1.17.38.11] CAKDRGKSGSYGLDHW in-frame + cctctgggttcaccttcagtgactactacatgggctggatccgccaggctccagggaaggggctggagtggattgcgtacattagtggtagtggtgataccatatactacgcagactctgtgaagggccgattcaccatctccagggtcaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggccgtgtattactgtgcgaaagatcgggggaaaagtgggagctacggacttgaccactggggccagggaaccctggtcaccgtctcctcagcatccccggaccagccccaaggtcttccgctgagcctcgacagcaccccccaagcatgggaacgtggtccgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 71 68 [291, 292] a81>g|a118>g,S40>G(+ + -)|g157>a,V53>I(+ - +)|t160>g,S54>A(- + -)|a162>g,S54>A(- + -)|a172>g,S58>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)|a242>t,D81>V(- - -)|a263>c,Y88>S(+ - -)|g317>a,R106>K(+ + +)| a81>g| a118>g,S40>G(+ + -)|g157>a,V53>I(+ - +)|t160>g,S54>A(- + -)|a162>g,S54>A(- + -)| a172>g,S58>G(+ + -)|a187>g,S63>D(- - -)|g188>a,S63>D(- - -)| a242>t,D81>V(- - -)|a263>c,Y88>S(+ - -)| g317>a,R106>K(+ + +)| 320 226 215 11 1 10 5 3 0 0 1 0 1 0 0 0 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 51 51 47 4 0 4 2 1 0 0 0 0 0 0 0 0 0 1 30 24 21 3 0 3 2 1 0 0 0 0 0 0 0 0 0 0 117 114 112 2 0 2 0 0 0 0 1 0 1 0 0 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct gggttcaccttcagtgactactac atgggctggatccgccaggctccagggaaggggctggagtggattgcgtac attagtggtagtggtgataccata tactacgcagactctgtgaagggccgattcaccatctccagggtcaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggccgtgtattactgt gcgaaagatcgggggaaaagtgggagctacggacttgaccac AKDRGKSGSYGLDH IGHV3-11 IGHD1-26 IGHJ4 gggttcaccttcagtgactactac atgggctggatccgccaggctccagggaaggggctggagtggattgcgtac attagtggtagtggtgataccata tactacgcagactctgtgaagggccgattcaccatctccagggtcaacgccaagaactcactgtctctgcaaatgaacagcctgagagccgaggacacggccgtgtattactgt gcgaaagatcgggggaaaagtgggagctacggacttgaccac unmatched 10 213 7 3 2 2 5 8 0 1 6 3 JY8QFUQ01BMPYC 14206 productive Homsap IGHV3-74*03 F 909 91.24 198/217 nt NA Homsap IGHJ5*02 F 147 76.47 39/51 nt Homsap IGHD6-19*01 F 1 8 8 14 8.8.14 [1.17.38.11] CAKVDSDGWHGTIDRW in-frame + ctctggattcaccttcagtaattactggatgtactgggtccgccaagttccagggaaggggctggtgtgggtcgcccgtattaataacgatgggagtagcaaaacttacgcagactccgtgaggggccgattcaccatctccagagacaacgccaagaacacactgtttctgcaaatgaacagtctgagaggcgaggacacggcttcatattattgtgcaaaagtggatagcgatggctggcacggaacaatagaccgctggggccagggagtcctggtcaccgtctccgcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [290, 291] g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|c118>t,H40>Y(+ - -)|c134>t,A45>V(+ - +)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)|g173>a,S58>N(- - -)|t174>c,S58>N(- - -)|c194>a,T65>K(- - -)|g198>t|g204>a|a215>g,K72>R(+ + +)|g258>a|a263>t,Y88>F(- + -)|c287>g,A96>G(- + -)|g301>t,V101>S(- - -)|t302>c,V101>S(- - -)|g303>a,V101>S(- - -)|c309>t|g317>a,R106>K(+ + +)| g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| c118>t,H40>Y(+ - -)|c134>t,A45>V(+ - +)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)| g173>a,S58>N(- - -)|t174>c,S58>N(- - -)|c194>a,T65>K(- - -)| g198>t|g204>a|a215>g,K72>R(+ + +)|g258>a|a263>t,Y88>F(- + -)|c287>g,A96>G(- + -)|g301>t,V101>S(- - -)|t302>c,V101>S(- - -)|g303>a,V101>S(- - -)|c309>t| g317>a,R106>K(+ + +)|a320>t| 319 (320) 224 (225) 204 20 (21) 4 (5) 16 1 6 4 2 1 1 1 (2) 0 0 1 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 51 51 47 4 0 4 0 0 2 0 1 0 0 0 0 0 0 1 30 24 21 3 0 3 0 1 0 1 0 1 0 0 0 0 0 0 117 114 104 10 4 6 1 3 1 1 0 0 1 0 0 1 2 0 7 (8) 7 6 1 (2) 0 (1) 1 0 1 0 0 0 0 0 (1) 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtaattactgg atgtactgggtccgccaagttccagggaaggggctggtgtgggtcgcccgt attaataacgatgggagtagcaaa acttacgcagactccgtgaggggccgattcaccatctccagagacaacgccaagaacacactgtttctgcaaatgaacagtctgagaggcgaggacacggcttcatattattgt gcaaaagtggatagcgatggctggcacggaacaatagaccgc AKVDSDGWHGTIDR IGHV3-74 IGHD6-19 IGHJ5 ggattcaccttcagtaattactgg atgtactgggtccgccaagttccagggaaggggctggtgtgggtcgcccgt attaataacgatgggagtagcaaa acttacgcagactccgtgaggggccgattcaccatctccagagacaacgccaagaacacactgtttctgcaaatgaacagtctgagaggcgaggacacggcttcatattattgt gcaaaagtggatagcgatggctggcacggaacaatagaccgc unmatched 19 213 12 7 9 13 3 6 4 0 10 5 JY8QFUQ01BMULR 12865 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 968 94.04 205/218 nt NA Homsap IGHJ4*02 F 222 95.83 46/48 nt Homsap IGHD6-6*01 F 2 8 8 16 8.8.16 [1.17.38.11] CAFSRALTGKWIDYLDHW in-frame + cctctggattcatcttcagcaactactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtattactggtgatgggagtaacccaatctacgcggaccccgtgaagggtcgattcaccatctccagagacaacgccaagaacacactatatctgcaaatgaacagtctgagagtcgaggacacggctgtgtattactgtgcctttagtagagcactcacggggaagtggatcgactaccttgaccactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgacgcctctgcagcacccagccagatgggaacgtgtcatcgctgcctggtccagggcttcttccc NA unmatched, IGA1 57 68 [297, 298] c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)|a170>c,N57>T(- + -)|a172>g,S58>G(+ + -)|g191>a,S64>N(- - -)|a193>c,T65>P(+ + -)|g197>t,S66>I(- - -)|t208>c,S70>P(+ - -)|c222>t|g258>a|g261>a|c287>t,A96>V(+ - +)| c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)| a170>c,N57>T(- + -)|a172>g,S58>G(+ + -)|g191>a,S64>N(- - -)|a193>c,T65>P(+ + -)| g197>t,S66>I(- - -)|t208>c,S70>P(+ - -)|c222>t|g258>a|g261>a|c287>t,A96>V(+ - +)| a315>c|a316>t,R106>F(- - -)|g317>t,R106>F(- - -)|a318>t,R106>F(- - -)|g319>a|a320>g| 314 (320) 220 (226) 207 13 (19) 4 (7) 9 (12) 1 (2) 4 (5) 3 2 2 (3) 0 0 (2) 0 0 0 1 (2) 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 1 1 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 1 0 0 2 0 0 0 0 0 0 0 117 114 108 6 3 3 0 2 2 1 0 0 0 0 0 0 1 0 2 (8) 2 2 0 (6) 0 (3) 0 (3) 0 (1) 0 (1) 0 0 0 (1) 0 0 (2) 0 0 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcatcttcagcaactactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attactggtgatgggagtaaccca atctacgcggaccccgtgaagggtcgattcaccatctccagagacaacgccaagaacacactatatctgcaaatgaacagtctgagagtcgaggacacggctgtgtattactgt gcctttagtagagcactcacggggaagtggatcgactaccttgaccac AFSRALTGKWIDYLDH IGHV3-74 IGHD6-6 IGHJ4 ggattcatcttcagcaactactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attactggtgatgggagtaaccca atctacgcggaccccgtgaagggtcgattcaccatctccagagacaacgccaagaacacactatatctgcaaatgaacagtctgagagtcgaggacacggctgtgtattactgt gcctttagtagagcactcacggggaagtggatcgactaccttgaccac unmatched 13 213 10 3 7 8 3 5 3 1 3 6 JY8QFUQ01BNJBB 16471 productive (see comment) Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 827 87.44 188/215 nt 86.98 187/215 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD2-21*02 F 3 8 7 15 8.7.15 [1.17.38.11] CARGRTVVTVIDYIDYW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 28 of V-REGION: 'A' (1 nucleotides at position 11 to 11 in the user submitted sequence), (cause frameshift); tctctgggggActccattagtggttactattggacgtggatccggcagaccccagggaagggactggagtggattggaaatgtccattacactgggagtaccaagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgtgcgagaggacgaacggtggtgactgttattgactacattgactattggggtcagggagccctggtcagcgtctcctcagcatcccctgaccagccccaaggtcttcccgctgagcctctgcagcacccccctaagatgggaaccgtggtcgtcgcatgcctggctccagggcttcttcccccag NA unmatched, IGA1 29 68 [293, 295] t81>g|c90>t|a106>g,S36>G(+ + -)|c114>t|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>a,P45>T(+ + -)|g162>a|t163>a,Y55>N(- - -)|a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|c192>t|c198>g,N66>K(+ - -)|a203>g,N68>S(- - -)|a234>g,I78>M(+ + -)|a240>t|c245>t,T82>M(- - -)|a251>g,K84>R(+ + +)|t262>a,S88>T(+ - +)|g267>c|g270>a|c271>t|g275>c,S92>T(+ - +)|g282>a|a283>g,T95>A(- - -)|g303>c| t81>g|c90>t|a106>g,S36>G(+ + -)|c114>t| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c133>a,P45>T(+ + -)|g162>a|t163>a,Y55>N(- - -)| a166>g,I56>V(+ - +)|t169>c,Y57>H(+ - -)|g176>c,S59>T(+ - +)|c192>t| c198>g,N66>K(+ - -)|a203>g,N68>S(- - -)|a234>g,I78>M(+ + -)|a240>t|c245>t,T82>M(- - -)|a251>g,K84>R(+ + +)|t262>a,S88>T(+ - +)|g267>c|g270>a|c271>t|g275>c,S92>T(+ - +)|g282>a|a283>g,T95>A(- - -)|g303>c| a320>g| 319 (320) 222 (223) 195 27 (28) 11 (12) 16 6 (7) 3 5 1 0 1 1 2 5 2 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 3 1 1 0 2 0 0 0 0 0 0 0 0 1 51 51 46 5 1 4 0 1 0 0 0 1 0 1 1 1 0 0 30 21 17 4 1 3 1 0 1 1 0 0 0 0 1 0 0 0 117 114 100 14 6 8 4 2 2 0 0 0 1 1 3 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct gggggctccattagtggttactat tggacgtggatccggcagaccccagggaagggactggagtggattggaaat gtccattacactgggagtacc aagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttattgactacattgactat ARGRTVVTVIDYIDY IGHV4-59 IGHD2-21 IGHJ4 gggggctccattagtggttactat tggacgtggatccggcagaccccagggaagggactggagtggattggaaat gtccattacactgggagtacc aagtacagcccctccctcaagagtcgagtcaccatgtcagttgacatgtccaggaaccagttcaccctcaaattgacctctgtagccgctgcggacacggccgtctattactgt gcgagaggacgaacggtggtgactgttattgactacattgactat unmatched 27 210 15 12 8 16 7 11 7 4 12 4 JY8QFUQ01BNJGF 7332 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 819 86.64 188/217 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-16*02 F 2 8 8 9 8.8.9 [1.17.38.11] CTGLTYPDDYW in-frame + gtctggatttaccttcagtaagttctggatgcattgggtccgccaagctccagggaaggggctgacttgggtctcacgtattaatcctgatgggactatcacgaactacacggactccgtgaggggccgattcatcacttccagagacaacgccaagaacacagtatatctgcagatgaacagtctgcgagtcgaggacacaggtgtatattactgtacaggactgacttatccagacgactactggggccagggatgtttggccaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [275, 276] c75>g|c84>t|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a110>t,Y37>F(- + -)|c120>t|g151>a,V51>T(- - -)|t152>c,V51>T(- - -)|g153>t,V51>T(- - -)|a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g188>c,S63>T(+ - +)|g191>t,S64>I(- - -)|a195>g|g197>a,S66>N(- - -)|g202>a,A68>T(- - -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|t233>c,I78>T(- - -)|c234>t,I78>T(- - -)|g258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a270>g|a283>c|c287>t,A96>V(+ - +)|g297>a|c299>g,A100>G(- + -)|g303>a|g313>a,A105>T(- - -)|a316>g,R106>G(- - -)| c75>g| c84>t|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a110>t,Y37>F(- + -)| c120>t|g151>a,V51>T(- - -)|t152>c,V51>T(- - -)|g153>t,V51>T(- - -)| a172>c,S58>P(+ - -)|g173>c,S58>P(+ - -)|g188>c,S63>T(+ - +)|g191>t,S64>I(- - -)|a195>g| g197>a,S66>N(- - -)|g202>a,A68>T(- - -)|a215>g,K72>R(+ + +)|c230>t,T77>I(- - -)|t233>c,I78>T(- - -)|c234>t,I78>T(- - -)|g258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a270>g|a283>c|c287>t,A96>V(+ - +)|g297>a|c299>g,A100>G(- + -)|g303>a| g313>a,A105>T(- - -)|a316>g,R106>G(- - -)|g319>c|a320>t| 318 (320) 223 (225) 192 31 (33) 9 (11) 22 4 9 5 2 2 0 1 (2) 0 2 (3) 4 2 0 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 20 4 1 3 0 1 1 0 0 0 1 0 0 1 0 0 51 51 47 4 1 3 0 1 1 1 0 0 0 0 0 0 1 0 30 24 19 5 1 4 1 0 0 0 1 0 0 0 2 0 1 0 117 114 99 15 5 10 2 6 3 1 1 0 0 0 0 2 0 0 6 (8) 6 4 2 (4) 0 (2) 2 1 1 0 0 0 0 0 (1) 0 0 (1) 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggatttaccttcagtaagttctgg atgcattgggtccgccaagctccagggaaggggctgacttgggtctcacgt attaatcctgatgggactatcacg aactacacggactccgtgaggggccgattcatcacttccagagacaacgccaagaacacagtatatctgcagatgaacagtctgcgagtcgaggacacaggtgtatattactgt acaggactgacttatccagacgactac TGLTYPDDY IGHV3-74 IGHD3-16 IGHJ4 ggatttaccttcagtaagttctgg atgcattgggtccgccaagctccagggaaggggctgacttgggtctcacgt attaatcctgatgggactatcacg aactacacggactccgtgaggggccgattcatcacttccagagacaacgccaagaacacagtatatctgcagatgaacagtctgcgagtcgaggacacaggtgtatattactgt acaggactgacttatccagacgactac unmatched 28 213 18 10 13 20 5 8 6 2 13 7 JY8QFUQ01BP3M1 17853 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 950 93.12 203/218 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [1.17.38.11] CTRDIGGRYSYW in-frame + cctccggattcacctttagtagttactggatgcactgggtccgccaagctccaggggagggactggtgtgggtctcacggactaatgaagatgggagtatcacaaactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgtacaagagacatcggtgggcgctactcctactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacacgcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccag NA unmatched, IGA2 64 84 [279] t78>c|c90>t|c108>t|a142>g,K48>E(+ - -)|g147>a|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|g313>a,A105>T(- - -)| t78>c| c90>t|c108>t| a142>g,K48>E(+ - -)|g147>a|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)| g313>a,A105>T(- - -)| 320 226 210 16 5 11 3 5 2 2 0 0 0 1 0 1 1 1 78 5 4 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 22 2 2 0 0 0 2 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 1 1 0 0 0 0 0 0 0 0 0 1 30 24 19 5 0 5 1 1 0 1 0 0 0 1 0 0 1 0 117 114 110 4 0 4 1 2 0 0 0 0 0 0 0 1 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctcc ggattcacctttagtagttactgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt acaagagacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttagtagttactgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtattactgt acaagagacatcggtgggcgctactcctac unmatched 14 213 10 4 6 8 4 6 2 2 5 5 JY8QFUQ01BPT8C 9512 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 951 92.73 204/220 nt NA Homsap IGHJ5*02 F (see comment) 138 74.51 38/51 nt Homsap IGHD3-10*01 F 2 10 7 13 10.7.13 [1.17.38.11] CARRGSYGSGSYYPW in-frame + Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctggtggcgccatcagcagtaatagttactactgggactggatccgccagcccccagggaaggggctggagtggattgggagtatgttttatactggggtcaccttctacaacccgtccctcaagagtcgagttaacatttccgtggacacgtccaagagccagttctccctgaggctgagctctgtgaccgccgcagacacggctgtgtatcactgtgcgagacgtggttcctatggttcggggagttattatccctggggccggggaaccctggtcatcgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [290, 291] t85>g,S29>A(- + -)|g104>a,S35>N(- - -)|g119>a,G40>D(- - -)|c168>g,I56>M(+ + -)|a170>t,Y57>F(- + -)|g176>c,S59>T(+ - +)|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)|a197>t,Y66>F(- + -)|c228>t|c230>a,T77>N(- + -)|a234>t|a240>g|a254>g,N85>S(- - -)|a269>g,K90>R(+ + +)|t307>c,Y103>H(+ - -)| t85>g,S29>A(- + -)|g104>a,S35>N(- - -)| g119>a,G40>D(- - -)| c168>g,I56>M(+ + -)|a170>t,Y57>F(- + -)|g176>c,S59>T(+ - +)|a190>g,S64>V(- - -)|g191>t,S64>V(- - -)| a197>t,Y66>F(- + -)|c228>t|c230>a,T77>N(- + -)|a234>t|a240>g|a254>g,N85>S(- - -)|a269>g,K90>R(+ + +)|t307>c,Y103>H(+ - -)| a320>g| 319 (320) 227 (228) 211 16 (17) 3 (4) 13 4 (5) 2 1 1 0 1 3 0 1 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 28 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 21 16 5 0 5 1 0 0 0 0 0 1 0 1 1 1 0 117 114 106 8 3 5 3 0 1 1 0 1 2 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtggcgccatcagcagtaatagttactac tgggactggatccgccagcccccagggaaggggctggagtggattgggagt atgttttatactggggtcacc ttctacaacccgtccctcaagagtcgagttaacatttccgtggacacgtccaagagccagttctccctgaggctgagctctgtgaccgccgcagacacggctgtgtatcactgt gcgagacgtggttcctatggttcggggagttattatccc ARRGSYGSGSYYP IGHV4-39 IGHD3-10 IGHJ5 ggtggcgccatcagcagtaatagttactac tgggactggatccgccagcccccagggaaggggctggagtggattgggagt atgttttatactggggtcacc ttctacaacccgtccctcaagagtcgagttaacatttccgtggacacgtccaagagccagttctccctgaggctgagctctgtgaccgccgcagacacggctgtgtatcactgt gcgagacgtggttcctatggttcggggagttattatccc unmatched 16 216 8 8 3 7 5 9 3 0 6 7 JY8QFUQ01BPXZS 7838 productive Homsap IGHV4-61*02 F 812 85.52 189/221 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-9*01 F 2 10 7 15 10.7.15 [1.17.38.11] CARCPNYNIGTRGFDYW in-frame + tttctggtgtctccatgagcaatgagtcctattactggacgtggatccggcagcccgtcgggaagggaccggagtggattgggcgcatctacaccagtgggagcaccaattataatccttccctcaagagtcgagtcaccatgtccttagacacgtccaagaggcagttctccctgaagttgacctctatgaccgccgcagacacggccacatatttctgtgcgaggtgtcccaattacaacattgggacccgggggtttgactactggggccagggaatcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [297, 298] c75>t|g83>t,G28>V(- - -)|c90>g,I30>M(+ + -)|g101>a,S34>N(- - -)|g104>a,G35>E(- - -)|t105>g,G35>E(- - -)|a106>t|g107>c|t108>c|c111>t|g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c137>t,A46>V(+ - +)|t149>c,L50>P(- - -)|t165>c|t171>c|c198>t|c201>t|c204>t|c207>t|a234>g,I78>M(+ + -)|a237>c|g238>t,V80>L(+ - +)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)|c271>t|g275>c,S92>T(+ - +)|g280>a,V94>M(+ - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)|a308>t,Y103>F(- + -)| c75>t| g83>t,G28>V(- - -)|c90>g,I30>M(+ + -)|g101>a,S34>N(- - -)|g104>a,G35>E(- - -)|t105>g,G35>E(- - -)|a106>t|g107>c|t108>c|c111>t| g119>c,S40>T(+ - +)|c120>g,S40>T(+ - +)|c137>t,A46>V(+ - +)|t149>c,L50>P(- - -)|t165>c| t171>c| c198>t|c201>t|c204>t|c207>t|a234>g,I78>M(+ + -)|a237>c|g238>t,V80>L(+ - +)|a254>g,N85>R(+ - -)|c255>g,N85>R(+ - -)|c271>t|g275>c,S92>T(+ - +)|g280>a,V94>M(+ - -)|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>a,V101>T(- - -)|a308>t,Y103>F(- + -)| a318>g|g319>t|a320>g| 317 (320) 226 (229) 194 32 (35) 13 (16) 19 2 (4) 5 8 5 1 0 2 0 3 3 2 (3) 1 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 30 21 9 4 5 0 2 1 1 0 0 1 0 1 1 1 1 51 51 46 5 1 4 0 0 1 2 0 0 0 0 1 1 0 0 30 21 20 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 98 16 6 10 2 3 5 1 1 0 1 0 1 1 1 0 5 (8) 5 5 0 (3) 0 (3) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|tacc,171-174(CDR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tttct ggtgtctccatgagcaatgagtcctattac tggacgtggatccggcagcccgtcgggaagggaccggagtggattgggcgc atctacaccagtgggagcacc aattataatccttccctcaagagtcgagtcaccatgtccttagacacgtccaagaggcagttctccctgaagttgacctctatgaccgccgcagacacggccacatatttctgt gcgaggtgtcccaattacaacattgggacccgggggtttgactac ARCPNYNIGTRGFDY IGHV4-61 IGHD3-9 IGHJ4 ggtgtctccatgagcaatgagtcctattac tggacgtggatccggcagcccgtcgggaagggaccggagtggattgggcgc atctacaccagtgggagcacc aattataatccttccctcaagagtcgagtcaccatgtccttagacacgtccaagaggcagttctccctgaagttgacctctatgaccgccgcagacacggccacatatttctgt gcgaggtgtcccaattacaacattgggacccgggggtttgactac unmatched 31 216 19 12 12 20 7 11 7 5 14 5 JY8QFUQ01BR9V1 13064 productive Homsap IGHV3-11*01 F 1008 96.31 209/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD5-18*01 F 3 8 8 18 8.8.18 [1.17.38.11] CVRRVWGYSYGSVSYYFEYW in-frame + ctctggattcaccttcagtgactactacatgaactggatccgccaggctccagggaaggggctggagtggctttcatacattagtggtagtggaactaccatatcctacgcagactctgtgaagggccgattcaccatctccagggacaacgccaggaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtgtattattgtgtgaggcgggtgtggggatatagttatggttccgtttcgtactactttgagtactggggccagggaaccctggtcaccgtctcctcagcatcccgaccagccccaaggtcttcccgctgagcctcgacagcacccccaagcatggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 74 [301, 300] g119>a,S40>N(- - -)|g157>c,V53>L(+ - +)|a172>g,S58>G(+ + -)|t186>a|g188>c,S63>T(+ - +)|a197>c,Y66>S(+ - -)|a251>g,K84>R(+ + +)|c309>t|c314>t,A105>V(+ - +)| g119>a,S40>N(- - -)|g157>c,V53>L(+ - +)| a172>g,S58>G(+ + -)|t186>a|g188>c,S63>T(+ - +)| a197>c,Y66>S(+ - -)|a251>g,K84>R(+ + +)|c309>t| c314>t,A105>V(+ - +)|a318>g|g319>c|a320>g| 317 (320) 222 (225) 213 9 (12) 2 (5) 7 2 (4) 1 2 0 1 0 0 1 2 (3) 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 30 24 21 3 1 2 1 0 0 0 0 0 0 1 1 0 0 0 117 114 111 3 1 2 1 0 1 0 1 0 0 0 0 0 0 0 5 (8) 5 4 1 (4) 0 (3) 1 0 (2) 0 1 0 0 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgactactac atgaactggatccgccaggctccagggaaggggctggagtggctttcatac attagtggtagtggaactaccata tcctacgcagactctgtgaagggccgattcaccatctccagggacaacgccaggaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtgtattattgt gtgaggcgggtgtggggatatagttatggttccgtttcgtactactttgagtac VRRVWGYSYGSVSYYFEY IGHV3-11 IGHD5-18 IGHJ4 ggattcaccttcagtgactactac atgaactggatccgccaggctccagggaaggggctggagtggctttcatac attagtggtagtggaactaccata tcctacgcagactctgtgaagggccgattcaccatctccagggacaacgccaggaactcactgtatctgcaaatgaacagcctgagagccgaggacacggccgtgtattattgt gtgaggcgggtgtggggatatagttatggttccgtttcgtactactttgagtac unmatched 8 213 4 4 2 4 2 4 1 1 4 2 JY8QFUQ01BRGSI 7034 productive Homsap IGHV3-48*01 F 999 95.85 208/217 nt NA Homsap IGHJ3*01 F 196 88 44/50 nt Homsap IGHD3-16*01 F 3 8 8 11 8.8.11 [1.17.38.11] CARVGATLSFDVW in-frame + ctctggattcaccctcagtagctataacatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagtattagtagtggtaccatatactatgcagactctgtgaagggccgattcaccatctccagggacaatgccgagaactcactgtatctgcaaatgagcagcctgagagccgacgacacggctgtgtattactgtgcgagagtgggagcgacgctttcttttgatgtctggggccaagggacaacggtcaccgtctcctcagcatccccggaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA unmatched, IGA2 57 84 [282, 283] t88>c,F30>L(+ - -)|g113>a,S38>N(- - -)|g173>t,S58>I(- - -)|a187>g,S63>G(+ + -)|c201>t|a240>g|a250>g,K84>E(+ - -)|a275>g,N92>S(- - -)|g291>c,E97>D(+ - +)| t88>c,F30>L(+ - -)|g113>a,S38>N(- - -)| g173>t,S58>I(- - -)|a187>g,S63>G(+ + -)| c201>t|a240>g|a250>g,K84>E(+ - -)|a275>g,N92>S(- - -)|g291>c,E97>D(+ - +)| a320>t| 319 (320) 224 (225) 215 9 (10) 2 (3) 7 4 1 1 1 0 0 0 (1) 0 1 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 1 0 1 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 1 0 0 0 0 0 0 0 0 0 1 0 117 114 109 5 2 3 3 0 1 0 0 0 0 0 1 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccctcagtagctataac atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtattagtagtggtaccata tactatgcagactctgtgaagggccgattcaccatctccagggacaatgccgagaactcactgtatctgcaaatgagcagcctgagagccgacgacacggctgtgtattactgt gcgagagtgggagcgacgctttcttttgatgtc ARVGATLSFDV IGHV3-48 IGHD3-16 IGHJ3 ggattcaccctcagtagctataac atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtattagtagtggtaccata tactatgcagactctgtgaagggccgattcaccatctccagggacaatgccgagaactcactgtatctgcaaatgagcagcctgagagccgacgacacggctgtgtattactgt gcgagagtgggagcgacgctttcttttgatgtc unmatched 9 213 7 2 2 4 5 5 2 0 3 4 JY8QFUQ01BRNFF 6021 productive Homsap IGHV4-4*02 F, or Homsap IGHV4-4*03 F or Homsap IGHV4-4*04 F or Homsap IGHV4-4*05 F 927 92.17 200/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD5-12*01 F 2 9 7 11 9.7.11 [1.17.38.11] CARASTLRYLDRW in-frame + ctctggtggctccatcagcagtgataattggtggagttgggtccgccagcccccagggaagggactggaatggattggggaaatatatcatagtgggagcacctactacaacccgtccctcaagagtcgagtcaccatatccctagacaagtccaagagtcaattcttcctggagctgaggtctgtgaccgccgcggacacggccgtatattattgtgcgagagcctccacactacgatatcttgaccgctggggccagggaaccccggtcaccgtctcctcagcatccccgaccagccccgaaggtcttcccgctgagcctctgcagcacccanccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA unmatched, IGA1 57 79 [282, 281] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c111>t|g147>a|g153>a|c168>a|a196>t,N66>Y(- - -)|a237>c|g238>c,V80>L(+ - +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|c263>t,S88>F(- - -)|a268>g,K90>E(+ - -)|c276>g,S92>R(- - -)|g303>a|c309>t| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c111>t| g147>a|g153>a| c168>a| a196>t,N66>Y(- - -)|a237>c|g238>c,V80>L(+ - +)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|c263>t,S88>F(- - -)|a268>g,K90>E(+ - -)|c276>g,S92>R(- - -)|g303>a|c309>t| a320>c| 319 (320) 224 (225) 207 17 (18) 8 (9) 9 3 5 4 0 1 (2) 1 1 0 1 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 27 24 3 1 2 1 1 1 0 0 0 0 0 0 0 0 0 51 51 49 2 2 0 0 2 0 0 0 0 0 0 0 0 0 0 30 21 20 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 117 114 103 11 4 7 2 2 3 0 1 0 1 0 1 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|aa,142-143(FR2)|aa,164-165(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|ta,108-109(CDR1)|tt,120-121(FR2)|tt,158-159(FR2)|ta,169-170(CDR2)|ta,174-175(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agtt,118-121(FR2)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggtggctccatcagcagtgataattgg tggagttgggtccgccagcccccagggaagggactggaatggattggggaa atatatcatagtgggagcacc tactacaacccgtccctcaagagtcgagtcaccatatccctagacaagtccaagagtcaattcttcctggagctgaggtctgtgaccgccgcggacacggccgtatattattgt gcgagagcctccacactacgatatcttgaccgc ARASTLRYLDR IGHV4-4 IGHD5-12 IGHJ4 ggtggctccatcagcagtgataattgg tggagttgggtccgccagcccccagggaagggactggaatggattggggaa atatatcatagtgggagcacc tactacaacccgtccctcaagagtcgagtcaccatatccctagacaagtccaagagtcaattcttcctggagctgaggtctgtgaccgccgcggacacggccgtatattattgt gcgagagcctccacactacgatatcttgaccgc unmatched 17 213 12 5 9 12 3 5 6 2 7 2 JY8QFUQ01BSGO4 14686 productive Homsap IGHV3-7*01 F 981 94.93 206/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-15*01 F 2 8 8 11 8.8.11 [1.17.38.11] CARSGSGSHLDYW in-frame + ctctagattcacctttagtaggttttggatgacctgggtccgccagggtccagggaaggggctggagtgggtggccaacataaagcaagttggaaatgagagatactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcattgtatctgcaaatgaacagcctgagagtcgacgacacggctgtgtattactgtgcgagaagtggtagtgggagccacttagattactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcc NA unmatched, IGA1 0 26 [281] g79>a,G27>R(- - -)|c108>g,S36>R(- - -)|a110>t,Y37>F(- + -)|g119>c,S40>T(+ - +)|c134>g,A45>G(- + -)|a176>t,D59>V(- - -)|g188>a,S63>N(- - -)|a194>g,K65>R(+ + +)|c259>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| g79>a,G27>R(- - -)|c108>g,S36>R(- - -)|a110>t,Y37>F(- + -)| g119>c,S40>T(+ - +)|c134>g,A45>G(- + -)| a176>t,D59>V(- - -)|g188>a,S63>N(- - -)|a194>g,K65>R(+ + +)| c259>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| g319>a|a320>g| 318 (320) 223 (225) 212 11 (13) 1 (3) 10 1 (2) 2 (3) 2 0 0 0 2 0 2 2 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 1 0 0 1 0 0 51 51 49 2 0 2 0 0 0 0 0 0 0 0 1 1 0 0 30 24 21 3 0 3 1 1 0 0 0 0 1 0 0 0 0 0 117 114 111 3 1 2 0 0 2 0 0 0 0 0 1 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct agattcacctttagtaggttttgg atgacctgggtccgccagggtccagggaaggggctggagtgggtggccaac ataaagcaagttggaaatgagaga tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcattgtatctgcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaagtggtagtgggagccacttagattac ARSGSGSHLDY IGHV3-7 IGHD2-15 IGHJ4 agattcacctttagtaggttttgg atgacctgggtccgccagggtccagggaaggggctggagtgggtggccaac ataaagcaagttggaaatgagaga tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcattgtatctgcaaatgaacagcctgagagtcgacgacacggctgtgtattactgt gcgagaagtggtagtgggagccacttagattac unmatched 11 213 5 6 4 8 1 3 1 0 4 6 JY8QFUQ01BT0O2 20754 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 150 79.17 38/48 nt Homsap IGHD1-26*01 F 3 8 8 10 8.8.10 [1.17.38.11] CTRDIGGRYSYW in-frame + ctccggattcacctttagtaattactggatgcactgggtccgccaagctccaggggagggactggtgtgggtctcacggactaatgaagatgggagtatcacaaactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtttctgcaaatgaacagtctcagagtcgaggacacggctgtttattactgtacaagagacatcggtgggcgctactcctactggggccagggagtcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [278, 279] t78>c|c90>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|a142>g,K48>E(+ - -)|g147>a|t165>g|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)|g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|a263>t,Y88>F(- + -)|g282>c|c287>t,A96>V(+ - +)|g303>t|g313>a,A105>T(- - -)| t78>c| c90>t|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| a142>g,K48>E(+ - -)|g147>a|t165>g| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g191>t,S64>I(- - -)| g197>a,S66>N(- - -)|a214>g,K72>E(+ - -)|g221>a,G74>D(- - -)|c257>g,T86>R(- - -)|a263>t,Y88>F(- + -)|g282>c|c287>t,A96>V(+ - +)|g303>t| g313>a,A105>T(- - -)| 320 225 204 21 6 15 3 6 3 2 0 0 1 1 1 1 2 1 78 4 3 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 1 2 0 0 0 0 0 0 0 0 0 51 51 48 3 2 1 1 1 0 0 0 0 0 0 0 0 0 1 30 24 19 5 0 5 1 1 0 1 0 0 0 1 0 0 1 0 117 114 106 8 2 6 1 2 1 0 0 0 1 0 1 1 1 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctcc ggattcacctttagtaattactgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtttctgcaaatgaacagtctcagagtcgaggacacggctgtttattactgt acaagagacatcggtgggcgctactcctac TRDIGGRYSY IGHV3-74 IGHD1-26 IGHJ4 ggattcacctttagtaattactgg atgcactgggtccgccaagctccaggggagggactggtgtgggtctcacgg actaatgaagatgggagtatcaca aactacgcggactccgtggaggaccgattcaccatctccagagacaacgccaagaacaggctgtttctgcaaatgaacagtctcagagtcgaggacacggctgtttattactgt acaagagacatcggtgggcgctactcctac unmatched 19 213 12 7 8 12 4 7 4 1 7 7 JY8QFUQ01BT4AX 11224 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 986 94.95 207/218 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD5-12*01 F 1 8 8 14 8.8.14 [1.17.38.11] CAKDLFQVSTTYFDYW in-frame + cctctggattcaccttcagtgactatcacatgtactgggtccgccaggctccaggcaaggggctggagtgggtggcagttatatcatatgatggaagtaataaatactatgtagactccgtgaagggccgattcaccatctccagagacaattccaagaatgcgctgtttctgcagatgaacagcctgagagctgacgacacggctgtgtattactgtgcgaaagacctctttcaggtgtctacgacttactttgactactggggccggggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctctccaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccggaaccggtgacggtgtcgtggaactc NA unmatched, IGG1 64 81 [292, 291] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g112>c,G38>H(+ - -)|g113>a,G38>H(+ - -)|c118>t,H40>Y(+ - -)|c203>t,A68>V(+ - +)|c255>t|a256>g,T86>A(- - -)|a263>t,Y88>F(- + -)|a270>g|g291>c,E97>D(+ - +)|g317>a,R106>K(+ + +)| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g112>c,G38>H(+ - -)|g113>a,G38>H(+ - -)| c118>t,H40>Y(+ - -)| c203>t,A68>V(+ - +)|c255>t|a256>g,T86>A(- - -)|a263>t,Y88>F(- + -)|a270>g|g291>c,E97>D(+ - +)| g317>a,R106>K(+ + +)| 320 226 214 12 2 10 3 3 3 0 0 0 1 0 2 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 2 0 0 0 0 0 0 1 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 2 4 2 0 2 0 0 0 1 0 1 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtgactatcac atgtactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcatatgatggaagtaataaa tactatgtagactccgtgaagggccgattcaccatctccagagacaattccaagaatgcgctgtttctgcagatgaacagcctgagagctgacgacacggctgtgtattactgt gcgaaagacctctttcaggtgtctacgacttactttgactac AKDLFQVSTTYFDY IGHV3-30 IGHD5-12 IGHJ4 ggattcaccttcagtgactatcac atgtactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcatatgatggaagtaataaa tactatgtagactccgtgaagggccgattcaccatctccagagacaattccaagaatgcgctgtttctgcagatgaacagcctgagagctgacgacacggctgtgtattactgt gcgaaagacctctttcaggtgtctacgacttactttgactac unmatched 11 213 8 3 5 7 3 4 2 0 5 4 JY8QFUQ01BT86M 16690 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 990 95.39 207/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 8 18 8.8.18 [1.17.38.11] CARAGYCNNGVCYSSYFDFW in-frame + ctctggattcaccttcagtagctactggatgcactgggtccgccaagctccggggaaggggctggtgtgggtctcacgtatgaatagtgatggcagtgacataaggtacgcggactccgtgaggggccgattcaccatctccagagacaacaccaagaacacgctgtatctacaaatgaacagtctgagagccgaggacacggctgtgtattactgtgcaagagcgggatattgtaataatggtgtatgctactccagttactttgacttctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA unmatched, IGA2 57 89 [302] a138>g|t168>g,I56>M(+ + -)|g186>c|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>t,T65>I(- - -)|c198>g,S66>R(- - -)|a215>g,K72>R(+ + +)|g247>a,A83>T(- - -)|g267>a| a138>g| t168>g,I56>M(+ + -)|g186>c|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|c194>t,T65>I(- - -)| c198>g,S66>R(- - -)|a215>g,K72>R(+ + +)|g247>a,A83>T(- - -)|g267>a| a320>c| 319 (320) 224 (225) 214 10 (11) 3 (4) 7 3 3 1 0 0 (1) 0 0 0 1 1 0 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 30 24 19 5 1 4 1 1 1 0 0 0 0 0 1 0 0 1 117 114 110 4 1 3 1 2 0 0 0 0 0 0 0 1 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccggggaaggggctggtgtgggtctcacgt atgaatagtgatggcagtgacata aggtacgcggactccgtgaggggccgattcaccatctccagagacaacaccaagaacacgctgtatctacaaatgaacagtctgagagccgaggacacggctgtgtattactgt gcaagagcgggatattgtaataatggtgtatgctactccagttactttgacttc ARAGYCNNGVCYSSYFDF IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccggggaaggggctggtgtgggtctcacgt atgaatagtgatggcagtgacata aggtacgcggactccgtgaggggccgattcaccatctccagagacaacaccaagaacacgctgtatctacaaatgaacagtctgagagccgaggacacggctgtgtattactgt gcaagagcgggatattgtaataatggtgtatgctactccagttactttgacttc unmatched 10 213 7 3 4 6 3 4 2 1 3 4 JY8QFUQ01BTQAH 21401 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*05 F or Homsap IGHV3-33*06 F 851 88.07 192/218 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD3-22*01 F 2 8 8 15 8.8.15 [1.17.38.11] CVRDDDTSGHYSLFKYW in-frame + cgtctggattctcctttagtgcatatggcatacactgggtccgccagactccaggcaaggggctggagtgggtggctgttatgtattttgatggagttagaacattttatgcagactccgtgaagggccgattcaccctctccaaagactattccaagaacacggtgcatctgcaaatgaacagcctgcgagccgaggacacggctgtatattactgtgtgagagatgatgataccagtggtcactactctctgtttaaatattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [294, 295] a85>t,T29>S(+ - +)|c90>t|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|c108>a,S36>A(- + -)|g117>a,M39>I(+ + -)|g133>a,A45>T(- - -)|a162>t|a168>g,I56>M(+ + -)|g170>a,W57>Y(- + -)|g171>t,W57>Y(- + -)|a173>t,Y58>F(- + -)|a187>g,S63>V(- - -)|g188>t,S63>V(- - -)|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)|a194>c,K65>T(- - -)|a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|a232>c,I78>L(+ + +)|g239>a,R80>K(+ + +)|a244>t,N82>Y(- - -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)|a283>c|g303>a|c314>t,A105>V(+ - +)| a85>t,T29>S(+ - +)|c90>t|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|c108>a,S36>A(- + -)| g117>a,M39>I(+ + -)|g133>a,A45>T(- - -)|a162>t| a168>g,I56>M(+ + -)|g170>a,W57>Y(- + -)|g171>t,W57>Y(- + -)|a173>t,Y58>F(- + -)|a187>g,S63>V(- - -)|g188>t,S63>V(- - -)|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)|a194>c,K65>T(- - -)| a197>t,Y66>F(- + -)|c198>t,Y66>F(- + -)|a232>c,I78>L(+ + +)|g239>a,R80>K(+ + +)|a244>t,N82>Y(- - -)|c259>g,L87>V(+ - +)|t262>c,Y88>H(+ - -)|a283>c|g303>a| c314>t,A105>V(+ - +)| 320 226 199 27 4 23 4 5 3 1 3 1 5 1 1 1 2 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 1 0 1 0 0 1 1 0 1 0 0 0 51 51 48 3 1 2 0 2 0 0 0 0 1 0 0 0 0 0 30 24 15 9 0 9 3 1 0 0 1 0 1 1 0 0 2 0 117 114 105 9 2 7 0 2 1 1 2 0 2 0 0 1 0 0 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cgtct ggattctcctttagtgcatatggc atacactgggtccgccagactccaggcaaggggctggagtgggtggctgtt atgtattttgatggagttagaaca ttttatgcagactccgtgaagggccgattcaccctctccaaagactattccaagaacacggtgcatctgcaaatgaacagcctgcgagccgaggacacggctgtatattactgt gtgagagatgatgataccagtggtcactactctctgtttaaatat VRDDDTSGHYSLFKY IGHV3-33 IGHD3-22 IGHJ4 ggattctcctttagtgcatatggc atacactgggtccgccagactccaggcaaggggctggagtgggtggctgtt atgtattttgatggagttagaaca ttttatgcagactccgtgaagggccgattcaccctctccaaagactattccaagaacacggtgcatctgcaaatgaacagcctgcgagccgaggacacggctgtatattactgt gtgagagatgatgataccagtggtcactactctctgtttaaatat unmatched 26 213 12 14 7 12 5 14 3 1 9 13 JY8QFUQ01BURMR 18488 productive Homsap IGHV3-11*01 F (see comment) 756 83.41 181/217 nt NA Homsap IGHJ5*02 F (see comment) 129 72.55 37/51 nt Homsap IGHD2-8*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARGRGRTNSPVDLW in-frame + Low V-REGION identity (83,41% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctggattcaccctcagtgactacagtatgagttgggtccgccaggctccagggaaggggctggagtgggtctcatacatcagccgaagtggaagtaatgtggaaactgcggactctgtgaggggccgattcaccgcctccagggacaccgccaataattcactgtttctgcggatgaatagcctgacagtcgaggacacggccctctattactgtgcgagaggtagggggcgcactaatagtccagttgacctctggggccagggaacccaggtcaccgtctccgtcagcatccccgaccagccccaaggtcttcccgctgagcctcctgcagcacccagccagatgggaacgtggtcatcgcctgcctagtccagggcttcttccccca NA unmatched, IGA1 64 84 [288, 289] t88>c,F30>L(+ - -)|t112>a,Y38>S(+ - -)|a113>g,Y38>S(+ - -)|c114>t,Y38>S(+ - -)|c120>t|a124>g,I42>V(+ - +)|t159>c|t168>c|t171>c|a172>c,S58>R(- - -)|t174>a,S58>R(- - -)|t186>a|c191>a,T64>N(- + -)|c192>t,T64>N(- + -)|a193>g,I65>V(+ - +)|a195>g,I65>V(+ - +)|t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|t199>a,Y67>T(+ - -)|a200>c,Y67>T(+ - -)|c201>t,Y67>T(+ - -)|a204>g|a215>g,K72>R(+ + +)|a232>g,I78>A(+ - +)|t233>c,I78>A(+ - +)|a245>c,N82>T(- + -)|g252>t,K84>N(+ - -)|c255>t|a263>t,Y88>F(- + -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>t|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)| t88>c,F30>L(+ - -)|t112>a,Y38>S(+ - -)|a113>g,Y38>S(+ - -)|c114>t,Y38>S(+ - -)| c120>t|a124>g,I42>V(+ - +)|t159>c| t168>c|t171>c|a172>c,S58>R(- - -)|t174>a,S58>R(- - -)|t186>a|c191>a,T64>N(- + -)|c192>t,T64>N(- + -)|a193>g,I65>V(+ - +)|a195>g,I65>V(+ - +)| t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|t199>a,Y67>T(+ - -)|a200>c,Y67>T(+ - -)|c201>t,Y67>T(+ - -)|a204>g|a215>g,K72>R(+ + +)|a232>g,I78>A(+ - +)|t233>c,I78>A(+ - +)|a245>c,N82>T(- + -)|g252>t,K84>N(+ - -)|c255>t|a263>t,Y88>F(- + -)|a269>g,Q90>R(+ - -)|a270>g,Q90>R(+ - -)|c276>t|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|g301>c,V101>L(+ - +)|g303>c,V101>L(+ - +)| a320>g| 319 (320) 224 (225) 188 36 (37) 8 (9) 28 9 (10) 0 7 5 3 2 1 4 3 0 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 0 1 1 0 0 0 1 0 0 0 0 51 51 48 3 2 1 1 0 1 1 0 0 0 0 0 0 0 0 30 24 15 9 3 6 2 0 1 2 1 1 0 2 0 0 0 0 117 114 94 20 3 17 5 0 4 1 2 1 1 1 3 0 1 1 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccctcagtgactacagt atgagttgggtccgccaggctccagggaaggggctggagtgggtctcatac atcagccgaagtggaagtaatgtg gaaactgcggactctgtgaggggccgattcaccgcctccagggacaccgccaataattcactgtttctgcggatgaatagcctgacagtcgaggacacggccctctattactgt gcgagaggtagggggcgcactaatagtccagttgacctc ARGRGRTNSPVDL IGHV3-11 IGHD2-8 IGHJ5 ggattcaccctcagtgactacagt atgagttgggtccgccaggctccagggaaggggctggagtgggtctcatac atcagccgaagtggaagtaatgtg gaaactgcggactctgtgaggggccgattcaccgcctccagggacaccgccaataattcactgtttctgcggatgaatagcctgacagtcgaggacacggccctctattactgt gcgagaggtagggggcgcactaatagtccagttgacctc unmatched 36 213 21 15 7 13 14 23 5 3 18 10 JY8QFUQ01BV9YG 13195 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 882 89.86 195/217 nt NA Homsap IGHJ5*02 F 237 96.08 49/51 nt Homsap IGHD6-19*01 F 2 8 8 17 8.8.17 [1.17.38.11] CAKSPLSVAGTRQNWFDPW in-frame + ctctgacttcacctttaatagctatgccatggcctgggtccgccaggctccagggaaggggctggagtgggtctcggctattggtgccagtggctacagcacatactacgcagactccgtcaagggccgcttcaccatctccagagaccattccaacagcacgctgcatctgcaaatgaacagcctgagagccgaagacacggccgtttattactgtgcgaaatcccctctatcagtggctggtacacgccaaaactggttcgacccctggggccagggaaccctggtcaccgtttcctcagcctccatcaagggccccatcggtcttccccccttggcaccctcctccaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA unmatched, IGG1 71 62 [303, 301] g80>a,G27>D(- - -)|a81>c,G27>D(- - -)|g104>a,S35>N(- - -)|c105>t,S35>N(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a162>g|a169>g,S57>G(+ + -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|t186>c|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|t189>c,G63>Y(+ - -)|g213>c|g225>c|a244>c,N82>H(- - -)|g252>c,K84>N(+ - -)|a254>g,N85>S(- - -)|t262>c,Y88>H(+ - -)|g291>a|a303>t| g80>a,G27>D(- - -)|a81>c,G27>D(- - -)|g104>a,S35>N(- - -)|c105>t,S35>N(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a162>g| a169>g,S57>G(+ + -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|t186>c|g187>t,G63>Y(+ - -)|g188>a,G63>Y(+ - -)|t189>c,G63>Y(+ - -)| g213>c|g225>c|a244>c,N82>H(- - -)|g252>c,K84>N(+ - -)|a254>g,N85>S(- - -)|t262>c,Y88>H(+ - -)|g291>a|a303>t| g319>t|a320>c| 318 (320) 223 (225) 201 22 (24) 6 (8) 16 4 4 1 4 2 (3) 0 1 0 5 0 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 2 1 0 1 0 0 0 0 0 0 0 51 51 48 3 1 2 2 0 0 0 0 0 0 0 1 0 0 0 30 24 17 7 1 6 1 1 0 3 0 0 0 0 1 0 1 0 117 114 106 8 4 4 1 1 0 1 1 0 1 0 3 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gacttcacctttaatagctatgcc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcggct attggtgccagtggctacagcaca tactacgcagactccgtcaagggccgcttcaccatctccagagaccattccaacagcacgctgcatctgcaaatgaacagcctgagagccgaagacacggccgtttattactgt gcgaaatcccctctatcagtggctggtacacgccaaaactggttcgacccc AKSPLSVAGTRQNWFDP IGHV3-23 IGHD6-19 IGHJ5 gacttcacctttaatagctatgcc atggcctgggtccgccaggctccagggaaggggctggagtgggtctcggct attggtgccagtggctacagcaca tactacgcagactccgtcaagggccgcttcaccatctccagagaccattccaacagcacgctgcatctgcaaatgaacagcctgagagccgaagacacggccgtttattactgt gcgaaatcccctctatcagtggctggtacacgccaaaactggttcgacccc unmatched 22 213 13 9 5 11 8 11 5 1 6 10 JY8QFUQ01BW9QL 17420 productive Homsap IGHV3-48*01 F, or Homsap IGHV3-48*02 F 999 95.85 208/217 nt NA Homsap IGHJ6*02 F 256 90.32 56/62 nt Homsap IGHD4-23*01 ORF 2 8 8 20 8.8.20 [1.17.38.11] CVRGDYGGAPGHYYDPYGLDVW in-frame - ctctggattcaccttcaggagttatatcatgaactgggtccgccaggctccagggaaggggctggagtggatttcatacattagtagtagtggtattatcatatactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgtgtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtctggggccaagggaccacggtcaaagtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG1 57 100 [309] t105>g,S35>R(- - -)|c108>t|g113>t,S38>I(- - -)|g157>a,V53>I(+ - +)|a184>g,S62>G(+ + -)|g188>t,S63>I(- - -)|c191>t,T64>I(- - -)|c287>t,A96>V(+ - +)|c309>t|c314>t,A105>V(+ - +)| t105>g,S35>R(- - -)|c108>t|g113>t,S38>I(- - -)| g157>a,V53>I(+ - +)| a184>g,S62>G(+ + -)|g188>t,S63>I(- - -)|c191>t,T64>I(- - -)| c287>t,A96>V(+ - +)|c309>t| c314>t,A105>V(+ - +)|a320>g| 319 (320) 224 (225) 214 10 (11) 2 (3) 8 1 (2) 1 5 0 0 0 0 0 0 0 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 0 1 0 0 0 0 0 0 0 1 1 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 1 0 1 0 0 0 0 0 0 0 1 0 117 114 112 2 1 1 0 0 2 0 0 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcaggagttatatc atgaactgggtccgccaggctccagggaaggggctggagtggatttcatac attagtagtagtggtattatcata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgt gtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtc VRGDYGGAPGHYYDPYGLDV IGHV3-48 IGHD4-23 IGHJ6 ggattcaccttcaggagttatatc atgaactgggtccgccaggctccagggaaggggctggagtggatttcatac attagtagtagtggtattatcata tactacgcagactctgtgaagggccgattcaccatctccagagacaatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattattgt gtgagaggggactacggtggcgcccccggacattactacgacccctacggtttggacgtc unmatched 9 213 6 3 5 7 1 2 1 1 2 5 JY8QFUQ01BWI2D 4664 productive Homsap IGHV3-30*01 F 819 86.64 188/217 nt NA Homsap IGHJ5*01 F, or Homsap IGHJ5*02 F (see comment) 156 78.43 40/51 nt Homsap IGHD5-12*01 F 3 8 8 12 8.8.12 [1.17.38.11] CARDFDTDDVGTYW in-frame + Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) ctctggattcatcttcgatagttatgctctgcactgggtccgccaggctccaggcaaggggctagagtgggtggcacttgtttcatatgatggaaaatataagcaatatgcagattctgtggagggccgattcaccatctccagagacaactccaagaacacaggatatctgcaaatgaacagcctgacatctgacgatacggctgtgtatttttgtgcgagagacttcgatactgacgacgtgggcacctactggggccggggaaccgtggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [284, 285] c86>t,T29>I(- - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c108>t|a115>c,M39>L(+ + -)|g163>c,V55>L(+ - +)|a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|g188>a,S63>K(- - -)|t189>a,S63>K(- - -)|a190>t,N64>Y(- - -)|a195>g|t196>c,Y66>Q(- - -)|c198>a,Y66>Q(- - -)|c201>t|c207>t|c210>t|a214>g,K72>E(+ - -)|t246>c|g258>a|c259>g,L87>G(- - -)|t260>g,L87>G(- - -)|g261>a,L87>G(- - -)|g284>c,R95>T(- - -)|g286>t,A96>S(- + -)|g291>c,E97>D(+ - +)|c294>t|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| c86>t,T29>I(- - -)|a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|c108>t| a115>c,M39>L(+ + -)|g163>c,V55>L(+ - +)| a166>g,I56>V(+ - +)|a168>t,I56>V(+ - +)|g188>a,S63>K(- - -)|t189>a,S63>K(- - -)|a190>t,N64>Y(- - -)|a195>g| t196>c,Y66>Q(- - -)|c198>a,Y66>Q(- - -)|c201>t|c207>t|c210>t|a214>g,K72>E(+ - -)|t246>c|g258>a|c259>g,L87>G(- - -)|t260>g,L87>G(- - -)|g261>a,L87>G(- - -)|g284>c,R95>T(- - -)|g286>t,A96>S(- + -)|g291>c,E97>D(+ - +)|c294>t|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| 320 225 196 29 8 21 4 4 7 2 1 1 3 1 3 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 2 0 0 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 0 0 1 0 0 0 1 0 0 0 30 24 18 6 1 5 2 1 0 0 0 0 2 1 0 0 0 0 117 114 97 17 6 11 1 2 5 2 0 1 1 0 2 1 1 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcgatagttatgct ctgcactgggtccgccaggctccaggcaaggggctagagtgggtggcactt gtttcatatgatggaaaatataag caatatgcagattctgtggagggccgattcaccatctccagagacaactccaagaacacaggatatctgcaaatgaacagcctgacatctgacgatacggctgtgtatttttgt gcgagagacttcgatactgacgacgtgggcacctac ARDFDTDDVGTY IGHV3-30 IGHD5-12 IGHJ5 ggattcatcttcgatagttatgct ctgcactgggtccgccaggctccaggcaaggggctagagtgggtggcactt gtttcatatgatggaaaatataag caatatgcagattctgtggagggccgattcaccatctccagagacaactccaagaacacaggatatctgcaaatgaacagcctgacatctgacgatacggctgtgtatttttgt gcgagagacttcgatactgacgacgtgggcacctac unmatched 29 213 17 12 11 17 6 12 6 2 13 8 JY8QFUQ01BXYLF 2040 productive (see comment) Homsap IGHV1-2*02 F 930 92.99 199/214 nt 92.52 198/214 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-19*01 F 1 8 8 12 8.8.12 [0.17.38.11] CARGPVSGWYTDYW in-frame - Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 57 of V-REGION: 'AACCCC' (6 nucleotides at position 80 to 85 in the user submitted sequence), (do not cause frameshift); tggatacaccttcaccgtctactatctattctgggtgcgacgggcccctggacaagggcttgagtggatgggatggatcAACCCCaaccctaagagtggtgacacacactatgcaccgaaattccagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggaactgaataggctgagatctgacgacacggccgtgtattactgtgcgagaggccctgtcagtggctggtacactgactactggggccagggaatcctggtcaccgtctcctcagcatcccgaccagcccaaggtcttcccgctgagcctcgacaacacccccaagtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 43 53 [285, 275] g107>t,G36>V(- - -)|a115>c,M39>L(+ + -)|g117>a,M39>L(+ + -)|c118>t,H40>F(- - -)|a119>t,H40>F(- - -)|a131>g,Q44>R(+ - -)|c177>g,N59>K(+ - -)|g191>a,G64>D(- - -)|a196>c,N66>H(- - -)|a206>c,Q69>P(- - -)|g210>a|t213>c|g270>a|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)| g107>t,G36>V(- - -)| a115>c,M39>L(+ + -)|g117>a,M39>L(+ + -)|c118>t,H40>F(- - -)|a119>t,H40>F(- - -)|a131>g,Q44>R(+ - -)| c177>g,N59>K(+ - -)|g191>a,G64>D(- - -)| a196>c,N66>H(- - -)|a206>c,Q69>P(- - -)|g210>a|t213>c|g270>a|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)| a320>g| 319 (320) 221 (222) 206 15 (16) 3 (4) 12 1 (2) 5 2 1 3 0 1 0 0 1 1 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 51 51 46 5 0 5 1 1 1 0 1 0 1 0 0 0 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 0 1 0 0 117 114 107 7 3 4 0 3 1 1 2 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,174-175(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggct,72-75(FR1)|ggct,106-109(CDR1)|ggct,146-149(FR2)|ggca,190-193(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,278-281(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|aacc,169-172(CDR2)|aact,196-199(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|tact,307-310(FR3)| t ggatacaccttcaccgtctactat ctattctgggtgcgacgggcccctggacaagggcttgagtggatgggatgg atcaaccctaagagtggtgacaca cactatgcaccgaaattccagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggaactgaataggctgagatctgacgacacggccgtgtattactgt gcgagaggccctgtcagtggctggtacactgactac ARGPVSGWYTDY IGHV1-2 IGHD6-19 IGHJ4 ggatacaccttcaccgtctactat ctattctgggtgcgacgggcccctggacaagggcttgagtggatgggatgg atcaaccctaagagtggtgacaca cactatgcaccgaaattccagggcagggtcaccatgaccagggacacgtccatcagcacagcctacatggaactgaataggctgagatctgacgacacggccgtgtattactgt gcgagaggccctgtcagtggctggtacactgactac unmatched 15 213 9 6 7 9 2 6 3 0 9 3 JY8QFUQ01BY231 19892 productive (see comment) Homsap IGHV4-31*02 F, or Homsap IGHV4-31*03 F or Homsap IGHV4-31*04 F 929 91.4 202/221 nt 90.95 201/221 nt Homsap IGHJ1*01 F 242 96.15 50/52 nt Homsap IGHD2-21*02 F 2 10 7 18 10.7.18 [1.17.38.11] CARSAYCGADCYSADYLQHW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 63 of V-REGION: 'G' (1 nucleotides at position 100 to 100 in the user submitted sequence), (cause frameshift); tctctggtggctccgtcagcagtaggggttactactggaactggatccgccagttcccagggaagggcctggagtggattgggaacatcttttacagtgGggggcacctacgacaacccgtccctcaggagtcgaatttctatatcattagacacgtctaagaaccaattctccctgaagttgacctctgtgaccgccgcggacacggccgtgtattactgtgcgagatcggcatactgtggtgctgactgctactcagctgattacctccagcactggggccagggcaccctggtcaccgtctcctcagcatccccgaccagccccaaaggtcttcccgctgagcctctgcacgcacccagccagatgggaacgtgtcatcgcctgctggtccagggcttcttccc NA unmatched, IGA1 71 63 [308, 309, 307] a88>g,I30>V(+ - +)|g103>a,G35>R(- - -)|t105>g,G35>R(- - -)|g119>a,S40>N(- - -)|c133>t,H45>F(- - -)|a134>t,H45>F(- - -)|t163>a,Y55>N(- - -)|a170>t,Y57>F(- + -)|a190>g,S64>G(+ + -)|t199>g,Y67>D(- - -)|a215>g,K72>R(+ + +)|g226>a,V76>I(+ - +)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|g238>t,V80>L(+ - +)|g258>a|c271>t|g275>c,S92>T(+ - +)|t285>c| a88>g,I30>V(+ - +)|g103>a,G35>R(- - -)|t105>g,G35>R(- - -)| g119>a,S40>N(- - -)|c133>t,H45>F(- - -)|a134>t,H45>F(- - -)|t163>a,Y55>N(- - -)| a170>t,Y57>F(- + -)|a190>g,S64>G(+ + -)| t199>g,Y67>D(- - -)|a215>g,K72>R(+ + +)|g226>a,V76>I(+ - +)|a229>t,T77>S(+ - +)|c231>t,T77>S(+ - +)|g238>t,V80>L(+ - +)|g258>a|c271>t|g275>c,S92>T(+ - +)|t285>c| g319>t|a320>c| 318 (320) 227 (229) 208 19 (21) 3 (5) 16 3 4 3 1 0 (1) 0 3 1 1 0 1 (2) 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 1 1 0 0 0 0 0 0 0 0 0 1 51 51 47 4 0 4 0 1 1 0 0 0 1 1 0 0 0 0 30 21 19 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 117 114 104 10 3 7 1 2 2 1 0 0 1 0 1 0 1 1 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 (1) 0 aa,40-41(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,228-229(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,69-70(FR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,227-228(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|ta,249-250(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggtt,106-109(CDR1)|agct,118-121(FR2)|agca,131-134(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agtt,225-228(FR3)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,69-72(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|tacc,228-231(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tgcc,285-288(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcagcagtaggggttactac tggaactggatccgccagttcccagggaagggcctggagtggattgggaac atcttttacagtgggggcacc tacgacaacccgtccctcaggagtcgaatttctatatcattagacacgtctaagaaccaattctccctgaagttgacctctgtgaccgccgcggacacggccgtgtattactgt gcgagatcggcatactgtggtgctgactgctactcagctgattacctccagcac ARSAYCGADCYSADYLQH IGHV4-31 IGHD2-21 IGHJ1 ggtggctccgtcagcagtaggggttactac tggaactggatccgccagttcccagggaagggcctggagtggattgggaac atcttttacagtgggggcacc tacgacaacccgtccctcaggagtcgaatttctatatcattagacacgtctaagaaccaattctccctgaagttgacctctgtgaccgccgcggacacggccgtgtattactgt gcgagatcggcatactgtggtgctgactgctactcagctgattacctccagcac unmatched 19 216 11 8 7 9 4 10 3 0 11 5 JY8QFUQ01BYGN8 15958 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 900 90.78 197/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD2-21*02 F 3 8 8 17 8.8.17 [1.17.38.11] CAKDLVVTSIGPQGIHGHW in-frame + ctctgggttcacctttagcagctatgccatgacttgggtccgccaggctccagggaaggggctggagtgggtctcaagtatcagttttagtggtgagagaacatattatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacagtacatttgcaaatggacagcctgagagccgaggacacggccgtatattactgtgcgaaagatctggtggtgactagtattggcccccagggaatccatggccactggggccagggaaccctggtcaccgtctcctcagcatcccccgaccagccccaaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA unmatched, IGA2 57 74 [301, 302] a81>g|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t168>c|g172>t,G58>F(- - -)|g173>t,G58>F(- - -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)|c198>t|c201>t|t246>c|g258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t262>c,Y88>H(+ - -)|c265>t|a274>g,N92>D(+ + -)| a81>g| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| t168>c|g172>t,G58>F(- - -)|g173>t,G58>F(- - -)|g188>a,G63>E(- - -)|t189>g,G63>E(- - -)|c192>a,S64>R(- - -)| c198>t|c201>t|t246>c|g258>a|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|t262>c,Y88>H(+ - -)|c265>t|a274>g,N92>D(+ + -)| 320 225 205 20 7 13 2 4 4 3 0 1 0 0 1 2 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 51 51 47 4 0 4 0 1 1 0 0 0 0 0 1 1 0 0 30 24 18 6 1 5 0 1 0 1 0 1 0 0 0 0 2 1 117 114 105 9 5 4 1 2 3 2 0 0 0 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct gggttcacctttagcagctatgcc atgacttgggtccgccaggctccagggaaggggctggagtgggtctcaagt atcagttttagtggtgagagaaca tattatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacagtacatttgcaaatggacagcctgagagccgaggacacggccgtatattactgt gcgaaagatctggtggtgactagtattggcccccagggaatccatggccac AKDLVVTSIGPQGIHGH IGHV3-23 IGHD2-21 IGHJ4 gggttcacctttagcagctatgcc atgacttgggtccgccaggctccagggaaggggctggagtgggtctcaagt atcagttttagtggtgagagaaca tattatgcagactccgtgaagggccggttcaccatctccagagacaactccaagaacacagtacatttgcaaatggacagcctgagagccgaggacacggccgtatattactgt gcgaaagatctggtggtgactagtattggcccccagggaatccatggccac unmatched 20 213 13 7 8 14 5 6 5 2 8 5 JY8QFUQ01C2NGE 8252 productive Homsap IGHV4-59*01 F, or Homsap IGHV4-59*07 F 930 92.99 199/214 nt NA Homsap IGHJ5*02 F 183 84.31 43/51 nt Homsap IGHD5-24*01 ORF 1 8 7 12 8.7.12 [1.17.38.11] CARAPRTATLLDPW in-frame + ctctggcgactccatcagtggtcactactggagctggatcaggcagcccccagggaagggactgcagtggattggttacatctatcacagtgggagcaccaactacaacccctccctcgagagtcgagtctccatttcagtagacacgtccaagaaccagttctccctgaggttgagttctgtgaccgctgcggacacggccgtgtattactgtgcgagagccccgaggacggctacacttctcgacccctggggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctctccaagagcacctctgggggccacagcggccctggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA unmatched, IGG1 64 81 [282, 281] t81>c|g83>a,G28>D(- - -)|a106>g,S36>G(+ + -)|t109>c,Y37>H(+ - -)|c127>a|g151>c,E51>Q(+ + -)|g162>t|t165>c|t172>c,Y58>H(+ - -)|a214>g,K72>E(+ - -)|a229>t,T77>S(+ - +)|a234>t|a269>g,K90>R(+ + +)|c271>t|c276>t| t81>c|g83>a,G28>D(- - -)|a106>g,S36>G(+ + -)|t109>c,Y37>H(+ - -)| c127>a|g151>c,E51>Q(+ + -)|g162>t|t165>c| t172>c,Y58>H(+ - -)| a214>g,K72>E(+ - -)|a229>t,T77>S(+ - +)|a234>t|a269>g,K90>R(+ + +)|c271>t|c276>t| a320>c| 319 (320) 221 (222) 206 15 (16) 7 (8) 8 3 1 2 4 0 (1) 1 2 0 1 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 1 1 0 2 0 0 0 0 0 0 0 0 51 51 47 4 3 1 0 0 0 1 0 1 0 0 1 0 1 0 30 21 20 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 117 114 108 6 3 3 2 0 2 0 0 0 2 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| ctct ggcgactccatcagtggtcactac tggagctggatcaggcagcccccagggaagggactgcagtggattggttac atctatcacagtgggagcacc aactacaacccctccctcgagagtcgagtctccatttcagtagacacgtccaagaaccagttctccctgaggttgagttctgtgaccgctgcggacacggccgtgtattactgt gcgagagccccgaggacggctacacttctcgacccc ARAPRTATLLDP IGHV4-59 IGHD5-24 IGHJ5 ggcgactccatcagtggtcactac tggagctggatcaggcagcccccagggaagggactgcagtggattggttac atctatcacagtgggagcacc aactacaacccctccctcgagagtcgagtctccatttcagtagacacgtccaagaaccagttctccctgaggttgagttctgtgaccgctgcggacacggccgtgtattactgt gcgagagccccgaggacggctacacttctcgacccc unmatched 15 210 10 5 3 6 7 9 6 1 4 4 JY8QFUQ01C2ROO 15773 productive Homsap IGHV3-7*01 F 1017 96.77 210/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSGFYSTGYW in-frame + ctctggattcacctttagtacctattggatgggctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaaacaagatggaagtgacaaatactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtctattactgtgcgattagtagtggtttttactcgacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagccctcgacacgcaccccccaagatgggaacgtggtcgtcgcagtgcctggtccccagggctcttcccccagga NA unmatched, IGA2 50 74 [281, 283] g107>c,S36>T(+ - +)|a118>g,S40>G(+ + -)|g171>a|g192>c,E64>D(+ - +)|c248>g,A83>G(- + -)|c265>t|g303>c| g107>c,S36>T(+ - +)| a118>g,S40>G(+ + -)| g171>a|g192>c,E64>D(+ - +)| c248>g,A83>G(- + -)|c265>t|g303>c| g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 316 (320) 221 (225) 214 7 (11) 3 (5) 4 (6) 1 (2) 1 (2) 1 0 0 0 0 (1) 0 3 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 51 51 50 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 117 114 111 3 2 1 0 0 1 0 0 0 0 0 1 1 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaacaagatggaagtgacaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgattagtagtggtttttactcgacgggctac AISSGFYSTGY IGHV3-7 IGHD3-22 IGHJ4 ggattcacctttagtacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaaacaagatggaagtgacaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacggcaagaactcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtctattactgt gcgattagtagtggtttttactcgacgggctac unmatched 7 213 3 4 2 6 1 1 2 1 2 2 JY8QFUQ01C3QHH 21700 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 968 94.04 205/218 nt 93.12 203/218 nt Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD2-8*02 F 2 8 8 20 8.8.20 [1.17.38.11] CARDLWLCSGGECYHRGGFENW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 41 of V-REGION: 'G' (1 nucleotides at position 37 to 37 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 85 of V-REGION: 'T' (1 nucleotides at position 162 to 162 in the user submitted sequence), (cause frameshift); cctctgggttcaccttcagtaactcctggatgcactGgggtccgccaagctccagggaaggggccggagtgggtctcacgtattaatagtgatgggagtaatacaatctacgcggactccgtgaagggccgactcaccatctccagagacaacgccaaaaaTcacggtgtatctgcaattgaacagtctgagagccgaggacacggctgtgtactactgtgcaagagatctgtggctttgtagtggtggtgaatgctatcatcggggtggttttgagaactggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccc NA unmatched, IGA2 57 89 [311, 312] a81>g|g107>a,S36>N(- - -)|a110>c,Y37>S(+ - -)|t149>c,L50>P(- - -)|t152>a,V51>E(- + -)|g191>a,S64>N(- - -)|c192>t,S64>N(- - -)|g197>t,S66>I(- - -)|t226>c,F76>L(+ - -)|g252>a|c259>g,L87>V(+ - +)|a271>t,M91>L(+ + -)|t306>c| a81>g|g107>a,S36>N(- - -)|a110>c,Y37>S(+ - -)| t149>c,L50>P(- - -)|t152>a,V51>E(- + -)| g191>a,S64>N(- - -)|c192>t,S64>N(- - -)| g197>t,S66>I(- - -)|t226>c,F76>L(+ - -)|g252>a|c259>g,L87>V(+ - +)|a271>t,M91>L(+ + -)|t306>c| 320 226 213 13 3 10 1 3 1 3 1 0 1 1 0 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 1 1 0 0 1 0 0 0 0 0 0 0 51 51 49 2 0 2 0 0 0 1 0 0 0 1 0 0 0 0 30 24 22 2 0 2 0 1 1 0 0 0 0 0 0 0 0 0 117 114 108 6 2 4 0 1 0 2 0 0 1 0 0 1 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct gggttcaccttcagtaactcctgg atgcactgggtccgccaagctccagggaaggggccggagtgggtctcacgt attaatagtgatgggagtaataca atctacgcggactccgtgaagggccgactcaccatctccagagacaacgccaaaaacacggtgtatctgcaattgaacagtctgagagccgaggacacggctgtgtactactgt gcaagagatctgtggctttgtagtggtggtgaatgctatcatcggggtggttttgagaac ARDLWLCSGGECYHRGGFEN IGHV3-74 IGHD2-8 IGHJ3 gggttcaccttcagtaactcctgg atgcactgggtccgccaagctccagggaaggggccggagtgggtctcacgt attaatagtgatgggagtaataca atctacgcggactccgtgaagggccgactcaccatctccagagacaacgccaaaaacacggtgtatctgcaattgaacagtctgagagccgaggacacggctgtgtactactgt gcaagagatctgtggctttgtagtggtggtgaatgctatcatcggggtggttttgagaac unmatched 13 213 8 5 4 6 4 7 2 1 6 4 JY8QFUQ01C4MHW 6619 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 981 94.93 206/217 nt 94.47 205/217 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD1-26*01 F 1 8 8 9 8.8.9 [1.17.38.11] CTREVGATDYW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 69 of V-REGION: 'A' (1 nucleotides at position 114 to 114 in the user submitted sequence), (cause frameshift); ctctggattcaccttcagtagctactggatgcattgggtccgccaagctccagggaaggggctggagtgggtctcacgtattcatagtgatgggactaccacatactacgcggAactccgtgaagggccgattcaccatctccagagacaacgccaggaacacgttgtatctgcaattgaacagtctgagagccgaggacacggctgtgtattattgtacaagagaggtgggagctaccgactactggggccagggaaccctggtcaccgtctcctcagcatcccctgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 79 [277, 278] c120>t|t152>a,V51>E(- + -)|a169>c,N57>H(- - -)|g188>c,S63>T(+ - +)|g191>c,S64>T(+ - +)|a196>t,S66>Y(+ - -)|g197>a,S66>Y(+ - -)|a251>g,K84>R(+ + +)|c259>t|a271>t,M91>L(+ + -)|c309>t|g313>a,A105>T(- - -)| c120>t|t152>a,V51>E(- + -)| a169>c,N57>H(- - -)|g188>c,S63>T(+ - +)|g191>c,S64>T(+ - +)| a196>t,S66>Y(+ - -)|g197>a,S66>Y(+ - -)|a251>g,K84>R(+ + +)|c259>t|a271>t,M91>L(+ + -)|c309>t| g313>a,A105>T(- - -)| 320 225 213 12 3 9 1 2 3 0 1 0 2 1 2 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 0 0 1 0 0 0 0 1 0 0 0 0 30 24 21 3 0 3 0 0 0 0 1 0 0 0 2 0 0 0 117 114 108 6 2 4 1 1 2 0 0 0 2 0 0 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcattgggtccgccaagctccagggaaggggctggagtgggtctcacgt attcatagtgatgggactaccaca tactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaggaacacgttgtatctgcaattgaacagtctgagagccgaggacacggctgtgtattattgt acaagagaggtgggagctaccgactac TREVGATDY IGHV3-74 IGHD1-26 IGHJ4 ggattcaccttcagtagctactgg atgcattgggtccgccaagctccagggaaggggctggagtgggtctcacgt attcatagtgatgggactaccaca tactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaggaacacgttgtatctgcaattgaacagtctgagagccgaggacacggctgtgtattattgt acaagagaggtgggagctaccgactac unmatched 11 213 5 6 4 6 1 5 3 0 5 3 JY8QFUQ01C5Q2O 11946 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 931 92.59 200/216 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 3 8 8 12 8.8.12 [1.17.38.11] CARDTGQWKDFDYW in-frame + tctggattcatcttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgtagtaatacgggggggactgacacagcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgtgcaagagatacggggcagtggaaggactttgactactggggccagggaaccctggtcaccgtctccttagcatcccccgaccagccccaaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA unmatched, IGA2 57 74 [285, 286] c86>t,T29>I(- - -)|c159>g|t167>g,I56>S(- - -)|g173>c,S58>T(+ - +)|t174>g,S58>T(+ - +)|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| c86>t,T29>I(- - -)| c159>g| t167>g,I56>S(- - -)|g173>c,S58>T(+ - +)|t174>g,S58>T(+ - +)|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| 320 224 208 16 3 13 3 1 1 0 1 0 0 0 3 3 1 3 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 30 24 16 8 0 8 2 1 0 0 0 0 0 0 2 0 0 3 117 114 108 6 2 4 1 0 0 0 1 0 0 0 1 2 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgt agtaatacgggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac ARDTGQWKDFDY IGHV3-74 IGHD6-19 IGHJ4 ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgt agtaatacgggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac unmatched 16 213 5 11 2 9 3 7 3 0 4 9 JY8QFUQ01C8QWZ 5146 productive Homsap IGHV1-3*01 F 966 94.86 203/214 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-8*01 F 2 8 8 9 8.8.9 [0.17.38.11] CARWNNDLDYW in-frame + tggatacaccttcagtacctatactatgaattgggtgcgccaggcccccggacaaaggcttgagtggatgggatggatcaacgctgaccttggcaacacaaaatattcacagaagttccagggcagactcaccattaccagggacacatccgcgaacacagcctacatggagctgagcagcctgacatctgaagacacggctgtgtattactgtgcgagatggaacaatgaccttgactattggggccagggaaccctagtcactgtctcttcagcatcccctgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggctccagggcttcttccccc NA unmatched, IGA2 57 79 [273, 274] c104>g,T35>S(+ - +)|g107>c,S36>T(+ - +)|g112>a,A38>T(- - -)|c118>a,H40>N(- - -)|g176>a,G59>D(- - -)|a184>c,N62>L(- - -)|a185>t,N62>L(- - -)|t189>c|g226>c,V76>L(+ - +)|g254>a,S85>N(- - -)|g284>c,R95>T(- - -)| c104>g,T35>S(+ - +)|g107>c,S36>T(+ - +)|g112>a,A38>T(- - -)| c118>a,H40>N(- - -)| g176>a,G59>D(- - -)|a184>c,N62>L(- - -)|a185>t,N62>L(- - -)|t189>c| g226>c,V76>L(+ - +)|g254>a,S85>N(- - -)|g284>c,R95>T(- - -)| g319>t|a320>g| 318 (320) 220 (222) 209 11 (13) 1 (3) 10 0 (1) 3 0 1 1 1 1 0 3 1 0 (1) 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 30 24 20 4 1 3 0 1 0 1 1 0 1 0 0 0 0 0 117 114 111 3 0 3 0 1 0 0 0 0 0 0 2 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|ta,262-263(FR3)|aa,290-291(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,11-12(FR1)|tt,62-63(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,120-121(FR2)|tt,149-150(FR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,201-202(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcagtacctatact atgaattgggtgcgccaggcccccggacaaaggcttgagtggatgggatgg atcaacgctgaccttggcaacaca aaatattcacagaagttccagggcagactcaccattaccagggacacatccgcgaacacagcctacatggagctgagcagcctgacatctgaagacacggctgtgtattactgt gcgagatggaacaatgaccttgactat ARWNNDLDY IGHV1-3 IGHD2-8 IGHJ4 ggatacaccttcagtacctatact atgaattgggtgcgccaggcccccggacaaaggcttgagtggatgggatgg atcaacgctgaccttggcaacaca aaatattcacagaagttccagggcagactcaccattaccagggacacatccgcgaacacagcctacatggagctgagcagcctgacatctgaagacacggctgtgtattactgt gcgagatggaacaatgaccttgactat unmatched 11 213 4 7 3 8 1 3 0 1 4 6 JY8QFUQ01C92F8 9845 productive (see comment) Homsap IGHV6-1*01 F, or Homsap IGHV6-1*02 F 1003 94.22 212/225 nt 93.78 211/225 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-22*01 F 2 10 9 14 10.9.14 [1.17.38.11] CARGGNYYDSGGLDYW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 38 of V-REGION: 'T' (1 nucleotides at position 32 to 32 in the user submitted sequence), (cause frameshift); tccggggacagtgtctctagcagcagtgttgTtttggaactggatcaggcagtccccattgagaggccttgagtggctgggaaggacattctacaggtccaggtggtataatgattattcattatctgtgaaaggtcgaataactatcaagccagacgcatccaagaaccagttctccctgcagctgaactctgtgactcccgaggacacggctgtatattactgtgcaagagggggtaattactatgatagtggtggtcttgactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagcccagatgggaacgtggtctngcctgcctggtccagggcttcttccccca NA unmatched, IGA1 57 84 [299, 300] a104>g,N35>S(- - -)|c110>t,A37>V(+ - +)|c113>t,A38>V(+ - +)|c140>t,S47>L(- - -)|a170>t,Y57>F(- + -)|a185>g,K62>R(+ + +)|g202>t,A68>S(- + -)|g205>t,V69>L(+ - +)|a220>g,S74>G(+ + -)|c231>t|c237>g,N79>K(+ - -)|a244>g,T82>A(- - -)|g303>a| a104>g,N35>S(- - -)|c110>t,A37>V(+ - +)|c113>t,A38>V(+ - +)| c140>t,S47>L(- - -)| a170>t,Y57>F(- + -)|a185>g,K62>R(+ + +)| g202>t,A68>S(- + -)|g205>t,V69>L(+ - +)|a220>g,S74>G(+ + -)|c231>t|c237>g,N79>K(+ - -)|a244>g,T82>A(- - -)|g303>a| a320>g| 319 (320) 232 (233) 219 13 (14) 2 (3) 11 4 (5) 1 4 0 0 0 1 0 0 1 2 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 27 3 0 3 1 0 2 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 30 27 25 2 0 2 1 0 0 0 0 0 1 0 0 0 0 0 117 114 107 7 2 5 2 1 1 0 0 0 0 0 0 1 2 0 7 (8) 7 7 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0 ta,5-6(FR1)|aa,40-41(FR1)|aa,103-104(CDR1)|aa,118-119(FR2)|aa,162-163(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,184-185(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|ta,206-207(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,227-228(FR3)|aa,235-236(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,5-6(FR1)|tt,114-115(CDR1)|tt,149-150(FR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,190-191(CDR2)|ta,192-193(CDR2)|tt,198-199(FR3)|ta,206-207(FR3)|ta,227-228(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggta,3-6(FR1)|agct,8-11(FR1)|agca,14-17(FR1)|agca,100-103(CDR1)|ggca,128-131(FR2)|ggct,155-158(FR2)|ggta,188-191(CDR2)|agta,204-207(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tgcc,69-72(FR1)|tgct,108-111(CDR1)|aact,118-121(FR2)|tact,169-172(CDR2)|aacc,228-231(FR3)|aacc,235-238(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|aact,274-277(FR3)|tact,307-310(FR3)| tcc ggggacagtgtctctagcagcagtgttgtt tggaactggatcaggcagtccccattgagaggccttgagtggctgggaagg acattctacaggtccaggtggtataat gattattcattatctgtgaaaggtcgaataactatcaagccagacgcatccaagaaccagttctccctgcagctgaactctgtgactcccgaggacacggctgtatattactgt gcaagagggggtaattactatgatagtggtggtcttgactat ARGGNYYDSGGLDY IGHV6-1 IGHD3-22 IGHJ4 ggggacagtgtctctagcagcagtgttgtt tggaactggatcaggcagtccccattgagaggccttgagtggctgggaagg acattctacaggtccaggtggtataat gattattcattatctgtgaaaggtcgaataactatcaagccagacgcatccaagaaccagttctccctgcagctgaactctgtgactcccgaggacacggctgtatattactgt gcaagagggggtaattactatgatagtggtggtcttgactat unmatched 13 222 9 4 5 8 4 5 2 0 6 5 JY8QFUQ01C966Y 5935 productive Homsap IGHV3-7*01 F 990 95.39 207/217 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-22*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAISSDFYSTGYW in-frame + ctctggattcagctttagaacctattggatgggctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagcacgatggaagtgacaaatactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcactgtttttgcaaatgaacagcctgagagccgaggacacggctgtgtactactgtgcgattagtagtgatttttactcgacgggctactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [281, 282] c86>g,T29>S(+ - +)|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)|a118>g,S40>G(+ + -)|a174>c,Q58>H(- + -)|g192>c,E64>D(+ - +)|a232>g,I78>V(+ - +)|a263>t,Y88>F(- + -)|c265>t|t306>c| c86>g,T29>S(+ - +)|t105>a,S35>R(- - -)|g107>c,S36>T(+ - +)| a118>g,S40>G(+ + -)| a174>c,Q58>H(- + -)|g192>c,E64>D(+ - +)| a232>g,I78>V(+ - +)|a263>t,Y88>F(- + -)|c265>t|t306>c| g317>t,R106>I(- + -)|a318>t,R106>I(- + -)|g319>a|a320>g| 316 (320) 221 (225) 211 10 (14) 2 (4) 8 (10) 2 (3) 0 (1) 1 1 1 0 1 (2) 1 2 1 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 0 0 0 0 1 1 1 0 0 51 51 50 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 1 0 0 0 1 0 0 0 117 114 110 4 2 2 1 0 1 1 0 0 1 0 0 0 0 0 4 (8) 4 4 0 (4) 0 (2) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagctttagaacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcacgatggaagtgacaaa tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcactgtttttgcaaatgaacagcctgagagccgaggacacggctgtgtactactgt gcgattagtagtgatttttactcgacgggctac AISSDFYSTGY IGHV3-7 IGHD3-22 IGHJ4 ggattcagctttagaacctattgg atgggctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcacgatggaagtgacaaa tactatgtggactctgtgaagggccgattcaccgtctccagagacaacgccaagaactcactgtttttgcaaatgaacagcctgagagccgaggacacggctgtgtactactgt gcgattagtagtgatttttactcgacgggctac unmatched 10 213 4 6 1 4 3 6 2 0 3 5 JY8QFUQ01C98A9 1689 productive Homsap IGHV1-18*01 F 966 94.86 203/214 nt NA Homsap IGHJ5*02 F (see comment) 174 82.35 42/51 nt Homsap IGHD3-10*01 F 2 8 8 16 8.8.16 [0.17.38.11] CARENYYTSGIHSGFRHW in-frame + Other possibilities: Homsap_IGHJ4*02 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) tggttacatctttaccacctatggtatcagttgggtgcgacaggcccctggacaagggcttgagtggatgggatggatcaacacttacaatggtaacacgaactatggacagaggatccagggcagagtcaccatgaccacagacacatccacgagcacagcctacatggagctgaggagcctgagatctgacgacacggccgtatattattgtgcgagagaaaactactatacttcgggaattcattccggcttcaggcactggggccagggaaccctggtcaccgtctcctcagctccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 84 [292, 293] c86>t,T29>I(- - -)|g107>c,S36>T(+ - +)|c120>t|g170>a,S57>N(- - -)|g172>a,A58>T(- - -)|a195>g|c203>g,A68>G(- + -)|a209>g,K70>R(+ + +)|c211>a,L71>I(+ + +)|g303>a|c309>t| c86>t,T29>I(- - -)|g107>c,S36>T(+ - +)| c120>t| g170>a,S57>N(- - -)|g172>a,A58>T(- - -)|a195>g| c203>g,A68>G(- + -)|a209>g,K70>R(+ + +)|c211>a,L71>I(+ + +)|g303>a|c309>t| 320 222 211 11 4 7 2 3 3 0 0 1 0 0 1 1 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 1 0 0 0 0 0 1 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 1 2 0 0 0 0 0 0 0 0 0 0 117 114 109 5 2 3 1 1 1 0 0 1 0 0 0 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|ta,175-176(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,5-6(FR1)|tt,75-76(FR1)|tt,81-82(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|tt,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| ggtt,3-6(FR1)|agct,8-11(FR1)|agct,24-27(FR1)|ggct,72-75(FR1)|ggtt,79-82(CDR1)|agct,106-109(CDR1)|ggta,112-115(CDR1)|agct,118-121(FR2)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)| agct,8-11(FR1)|agct,24-27(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|aact,196-199(FR3)|agct,209-212(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggttacatctttaccacctatggt atcagttgggtgcgacaggcccctggacaagggcttgagtggatgggatgg atcaacacttacaatggtaacacg aactatggacagaggatccagggcagagtcaccatgaccacagacacatccacgagcacagcctacatggagctgaggagcctgagatctgacgacacggccgtatattattgt gcgagagaaaactactatacttcgggaattcattccggcttcaggcac ARENYYTSGIHSGFRH IGHV1-18 IGHD3-10 IGHJ5 ggttacatctttaccacctatggt atcagttgggtgcgacaggcccctggacaagggcttgagtggatgggatgg atcaacacttacaatggtaacacg aactatggacagaggatccagggcagagtcaccatgaccacagacacatccacgagcacagcctacatggagctgaggagcctgagatctgacgacacggccgtatattattgt gcgagagaaaactactatacttcgggaattcattccggcttcaggcac unmatched 11 213 8 3 6 9 2 2 3 1 3 4 JY8QFUQ01CANL1 17456 productive Homsap IGHV3-9*02 F 896 90.37 197/218 nt NA Homsap IGHJ6*02 F 109 66.07 37/56 nt Homsap IGHD1-14*01 ORF 2 8 8 11 8.8.11 [1.17.38.11] CGKDMRPGGMDVW in-frame + cctccggattcacctctggaaagtatgccatgcactgggtccggcaagctccagggaaggacctggagtgggtctcaggcttgggtttggataatggtaggatagactacgcggactctgtgaagggccgattcaccatctccaaagacaacgccaagaattccctgtatctgcaaatgaacagcctgagagttgaggacacggccatgtattactgtggaaaagatatgcgaccgggcggtatggacgtctggggccaggggatcacggtcaccggtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagnacccccccaaggatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccga NA unmatched, IGA2 57 79 [283, 285, 275] t78>c|a104>g,D35>G(- - -)|t105>a,D35>G(- - -)|g106>a,D36>K(+ - -)|t108>g,D36>K(+ - -)|g146>a,G49>D(- - -)|t165>c|a166>t,I56>L(+ + +)|t168>g,I56>L(+ + +)|a169>g,S57>G(+ + -)|g173>t,W58>L(+ - -)|a175>g,N59>D(+ + -)|g185>a,S62>N(- - -)|c192>g,S64>R(- - -)|g197>a,G66>D(- - -)|t201>c|g239>a,R80>K(+ + +)|c255>t|t279>c|c287>t,A96>V(+ - +)|t301>a,L101>M(+ + -)|c314>g,A105>G(- + -)| t78>c| a104>g,D35>G(- - -)|t105>a,D35>G(- - -)|g106>a,D36>K(+ - -)|t108>g,D36>K(+ - -)| g146>a,G49>D(- - -)|t165>c| a166>t,I56>L(+ + +)|t168>g,I56>L(+ + +)|a169>g,S57>G(+ + -)|g173>t,W58>L(+ - -)|a175>g,N59>D(+ + -)|g185>a,S62>N(- - -)|c192>g,S64>R(- - -)| g197>a,G66>D(- - -)|t201>c|g239>a,R80>K(+ + +)|c255>t|t279>c|c287>t,A96>V(+ - +)|t301>a,L101>M(+ + -)| c314>g,A105>G(- + -)| 322 228 206 22 5 17 3 5 2 4 0 0 1 2 0 2 1 2 78 5 4 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 0 0 0 0 0 1 0 0 0 1 51 51 49 2 1 1 0 1 0 1 0 0 0 0 0 0 0 0 30 24 17 7 0 7 2 1 0 0 0 0 1 0 0 1 1 1 117 114 107 7 3 4 0 2 2 2 0 0 0 1 0 0 0 0 10 10 9 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 aa,2-3(FR1)|ta,38-39(FR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,175-176(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)|aa,317-318(CDR3)|ta,321-322(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,108-109(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|tt,171-172(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,301-302(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,321-322(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|ggca,46-49(FR1)|ggca,128-131(FR2)|agct,132-135(FR2)|ggta,163-166(FR2-CDR2)|agtt,169-172(CDR2)|ggta,187-190(CDR2)|ggct,196-199(FR3)|agct,285-288(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tgcc,111-114(CDR1)|agct,132-135(FR2)|aact,253-256(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctcc ggattcacctctggaaagtatgcc atgcactgggtccggcaagctccagggaaggacctggagtgggtctcaggc ttgggtttggataatggtaggata gactacgcggactctgtgaagggccgattcaccatctccaaagacaacgccaagaattccctgtatctgcaaatgaacagcctgagagttgaggacacggccatgtattactgt ggaaaagatatgcgaccgggcggtatggacgtc GKDMRPGGMDV IGHV3-9 IGHD1-14 IGHJ6 ggattcacctctggaaagtatgcc atgcactgggtccggcaagctccagggaaggacctggagtgggtctcaggc ttgggtttggataatggtaggata gactacgcggactctgtgaagggccgattcaccatctccaaagacaacgccaagaattccctgtatctgcaaatgaacagcctgagagttgaggacacggccatgtattactgt ggaaaagatatgcgaccgggcggtatggacgtc unmatched 20 213 13 7 7 9 6 11 4 0 5 11 JY8QFUQ01CD8ZK 20091 productive Homsap IGHV3-48*02 F, or Homsap IGHV3-48*04 F 815 86.24 188/218 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD2-21*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAKVGNLYFFEYW in-frame + cctctggattcagcttcaacagctacagcatgaactgggtccgccaggctccagggaagggactggaatggatctcatcaattagtaccgctggcaccaccataggctacgcagactctgtgaagggccgattcactatttccagagacaacgccaagaactcagtatctctgcagatggacagcctgagagacgaggacacggcggtatattactgtgccaaagtgggtaatttgtatttctttgaatactggggccggggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacaacaccccccaagtggtcgtcgcatgcctggtccagggcttcttcccccag NA unmatched, IGA2 64 68 [282, 273] c86>g,T29>S(+ - +)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|t111>c|g147>a|g153>a|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|a164>c,Y55>S(+ - -)|c165>a,Y55>S(+ - -)|g173>c,S58>T(+ - +)|t174>c,S58>T(+ - +)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|a184>g,S62>G(+ + -)|t186>c,S62>G(+ + -)|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)|t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c231>t|c234>t|t246>c|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a263>c,Y88>S(+ - -)|a270>g|a274>g,N92>D(+ + -)|t300>g|g303>a|g315>c|g317>a,R106>K(+ + +)| c86>g,T29>S(+ - +)|g104>a,S35>N(- - -)|t105>c,S35>N(- - -)|t111>c| g147>a|g153>a|g157>a,V53>I(+ - +)|t159>c,V53>I(+ - +)|a164>c,Y55>S(+ - -)|c165>a,Y55>S(+ - -)| g173>c,S58>T(+ - +)|t174>c,S58>T(+ - +)|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|a184>g,S62>G(+ + -)|t186>c,S62>G(+ + -)|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)| t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c231>t|c234>t|t246>c|c259>g,L87>V(+ - +)|g261>a,L87>V(+ - +)|a263>c,Y88>S(+ - -)|a270>g|a274>g,N92>D(+ + -)|t300>g|g303>a| g315>c|g317>a,R106>K(+ + +)|a320>t| 319 (320) 225 (226) 193 32 (33) 10 (11) 22 5 7 2 7 2 1 0 (1) 0 4 2 0 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 1 0 2 0 0 0 0 0 1 0 0 51 51 45 6 2 4 0 3 0 1 1 1 0 0 0 0 0 0 30 24 16 8 0 8 2 0 0 3 0 0 0 0 3 0 0 0 117 114 102 12 6 6 3 2 2 1 1 0 0 0 0 1 0 2 7 (8) 7 5 2 (3) 1 (2) 1 0 1 0 0 0 0 0 (1) 0 1 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| cctct ggattcagcttcaacagctacagc atgaactgggtccgccaggctccagggaagggactggaatggatctcatca attagtaccgctggcaccaccata ggctacgcagactctgtgaagggccgattcactatttccagagacaacgccaagaactcagtatctctgcagatggacagcctgagagacgaggacacggcggtatattactgt gccaaagtgggtaatttgtatttctttgaatac AKVGNLYFFEY IGHV3-48 IGHD2-21 IGHJ4 ggattcagcttcaacagctacagc atgaactgggtccgccaggctccagggaagggactggaatggatctcatca attagtaccgctggcaccaccata ggctacgcagactctgtgaagggccgattcactatttccagagacaacgccaagaactcagtatctctgcagatggacagcctgagagacgaggacacggcggtatattactgt gccaaagtgggtaatttgtatttctttgaatac unmatched 30 213 20 10 8 14 12 16 8 1 10 11 JY8QFUQ01CD9VK 15608 productive (see comment) Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 955 93.15 204/219 nt 92.69 203/219 nt Homsap IGHJ6*02 F 121 66.13 41/62 nt Homsap IGHD3-16*01 F 2 8 8 16 8.8.16 [2.17.38.11] CARRLNNYDSGEYAMDVW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 46 of V-REGION: 'C' (1 nucleotides at position 53 to 53 in the user submitted sequence), (cause frameshift); ggttctggatacacgtttatgaattactggatcggctgggtgcgccagatgcCccgggaaaggcctggagtggatggggatcatctatcctggtacctctgagaccagatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgcctacgtgcagtggagcagcctgaaggccccggacaccgccatatattactgtgcgaggcgactcaataattacgactcgggagagtacgctatggacgtctggggccaagggaccacggccaagggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGA1 14 5 [] g86>c,S29>T(+ - +)|c87>g,S29>T(+ - +)|c104>t,T35>M(- - -)|c105>g,T35>M(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)|g184>a,D62>T(- + -)|a185>c,D62>T(- + -)|t192>g,D64>E(+ - +)|c213>t|c234>t|t251>c,I84>T(- - -)|c265>g,L89>V(+ - +)|t289>c,S97>P(+ - -)|g303>a,M101>I(+ + -)| g86>c,S29>T(+ - +)|c87>g,S29>T(+ - +)|c104>t,T35>M(- - -)|c105>g,T35>M(- - -)|g107>a,S36>N(- - -)|c108>t,S36>N(- - -)| g184>a,D62>T(- + -)|a185>c,D62>T(- + -)|t192>g,D64>E(+ - +)| c213>t|c234>t|t251>c,I84>T(- - -)|c265>g,L89>V(+ - +)|t289>c,S97>P(+ - -)|g303>a,M101>I(+ + -)| a318>g|a320>g| 317 (320) 224 (227) 209 (210) 15 (17) 2 (4) 13 0 (2) 3 4 2 1 0 0 0 1 3 0 1 78 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 18 6 0 6 0 1 2 0 0 0 0 0 1 2 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 0 1 0 0 1 0 0 0 0 0 0 1 117 114 108 6 2 4 0 1 2 2 0 0 0 0 0 1 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| ggttct ggatacacgtttatgaattactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtacctctgagacc agatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgcctacgtgcagtggagcagcctgaaggccccggacaccgccatatattactgt gcgaggcgactcaataattacgactcgggagagtacgctatggacgtc ARRLNNYDSGEYAMDV IGHV5-51 IGHD3-16 IGHJ6 ggatacacgtttatgaattactgg atcggctgggtgcgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtacctctgagacc agatacagcccgtcctttcaaggccaggtcaccatttcagccgacaagtccaccagcaccgcctacgtgcagtggagcagcctgaaggccccggacaccgccatatattactgt gcgaggcgactcaataattacgactcgggagagtacgctatggacgtc unmatched 15 213 9 6 7 11 2 4 2 0 4 9 JY8QFUQ01CDZ5R 18206 productive (see comment) Homsap IGHV3-30*01 F 868 89.35 193/216 nt 88.89 192/216 nt Homsap IGHJ3*01 F 97 66 33/50 nt Homsap IGHD2-2*02 F 2 8 7 11 8.7.11 [1.17.38.11] CACGHMRDGYDVW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 64 of V-REGION: 1 nucleotides (from position 98 in the user submitted sequence), (cause frameshift) ctctggattcaccttcagtgcctttactatgcactgggtccgccaggctccaggcgagggactagagtgggtggcagctatatcatatgatggcagtaaaaatactatgcggactttgtgaagggccgattcaccatctccagagacaatcccaagagtacactgtatctacaaatgaacggcctgggaggtgatgacacggctttgtattactgtgcgtgcggccatatgcgggatggttatgatgtctggggcccaagggacaaaggtcaccgctcttcatcatgcccgatcccgacccaaggtccagttcctgccgtctgagcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA unmatched, IGA1 100 58 [288] a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a110>t,Y37>F(- + -)|g112>a,A38>T(- - -)|a142>g,K48>E(+ - -)|g147>a|t164>c,V55>A(+ - +)|a186>c|t192>a|c201>t|a204>g|c209>t,S70>F(- - -)|c210>t,S70>F(- - -)|t247>c,S83>P(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|g267>a|a277>g,S93>G(+ + -)|a283>g,R95>G(- - -)|c287>g,A96>G(- + -)|g291>t,E97>D(+ - +)|g301>t,V101>L(+ - +)| a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a110>t,Y37>F(- + -)|g112>a,A38>T(- - -)| a142>g,K48>E(+ - -)|g147>a|t164>c,V55>A(+ - +)| a186>c|t192>a| c201>t|a204>g|c209>t,S70>F(- - -)|c210>t,S70>F(- - -)|t247>c,S83>P(+ - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|g258>a|g267>a|a277>g,S93>G(+ + -)|a283>g,R95>G(- - -)|c287>g,A96>G(- + -)|g291>t,E97>D(+ - +)|g301>t,V101>L(+ - +)| a316>t,R106>C(- - -)|a318>c,R106>C(- - -)|a320>g| 315 (320) 219 (224) 196 (198) 23 (26) 7 (8) 16 (18) 6 (7) 4 4 2 1 (2) 0 1 (2) 1 1 1 2 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 0 0 0 0 1 0 1 0 0 0 51 51 48 3 1 2 1 1 0 1 0 0 0 0 0 0 0 0 30 23 21 2 2 0 0 0 0 0 1 0 0 1 0 0 0 0 117 114 100 14 4 10 4 2 4 1 0 0 0 0 0 1 2 0 3 (8) 3 3 (5) 0 (3) 0 (1) 0 (2) 0 (1) 0 0 0 0 (1) 0 0 (1) 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgcctttact atgcactgggtccgccaggctccaggcgagggactagagtgggtggcagct atatcatatgatggcagtaaaaa tactatgcggactttgtgaagggccgattcaccatctccagagacaatcccaagagtacactgtatctacaaatgaacggcctgggaggtgatgacacggctttgtattactgt gcgtgcggccatatgcgggatggttatgatgtc ACGHMRDGYDV IGHV3-30 IGHD2-2 IGHJ3 ggattcaccttcagtgcctttact atgcactgggtccgccaggctccaggcgagggactagagtgggtggcagct atatcatatgatggcagtaaaaa tactatgcggactttgtgaagggccgattcaccatctccagagacaatcccaagagtacactgtatctacaaatgaacggcctgggaggtgatgacacggctttgtattactgt gcgtgcggccatatgcgggatggttatgatgtc unmatched 23 212 16 7 8 12 8 11 5 2 12 4 JY8QFUQ01CE8P9 7842 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 918 91.71 199/217 nt NA Homsap IGHJ4*02 F (see comment) 159 81.25 39/48 nt Homsap IGHD1-26*01 F 1 8 8 15 8.8.15 [1.17.38.11] CGKPIVGATGREMPVSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) ctctggattcacctttagcaactatgccatgaactgggtccgccaggttccaggggaggggctggagtgggtctcagccattagtggcagtggtggtagcacattctacacagacgccttgcagggccgattcaccatctccagagacaattccaagaacacgttatatttgcaaatgaaaagcctgagagccggggacacggccgtgtattactgtgggaagccaatagtgggcgcaacaggtagagagatgcctgtttcctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [293, 294] g107>a,S36>N(- - -)|g119>a,S40>N(- - -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|t165>c|t174>c|a197>t,Y66>F(- + -)|g202>a,A68>T(- - -)|t208>g,S70>A(- + -)|g211>t,V71>L(+ - +)|a214>c,K72>Q(+ - -)|g225>a|c259>t|g261>a|c265>t|c276>a,N92>K(+ - -)|a290>g,E97>G(- - -)|a303>g|c314>g,A105>G(- + -)| g107>a,S36>N(- - -)| g119>a,S40>N(- - -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|t165>c| t174>c| a197>t,Y66>F(- + -)|g202>a,A68>T(- - -)|t208>g,S70>A(- + -)|g211>t,V71>L(+ - +)|a214>c,K72>Q(+ - -)|g225>a|c259>t|g261>a|c265>t|c276>a,N92>K(+ - -)|a290>g,E97>G(- - -)|a303>g| c314>g,A105>G(- + -)|a318>g|g319>c|a320>c| 317 (320) 222 (225) 203 19 (22) 7 (10) 12 3 (4) 5 3 2 1 (2) 1 1 0 0 (1) 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 47 4 1 3 1 1 1 1 0 0 0 0 0 0 0 0 30 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 102 12 5 7 2 3 2 0 1 1 1 0 0 0 1 1 5 (8) 5 4 1 (4) 0 (3) 1 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 1 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagcaactatgcc atgaactgggtccgccaggttccaggggaggggctggagtgggtctcagcc attagtggcagtggtggtagcaca ttctacacagacgccttgcagggccgattcaccatctccagagacaattccaagaacacgttatatttgcaaatgaaaagcctgagagccggggacacggccgtgtattactgt gggaagccaatagtgggcgcaacaggtagagagatgcctgtttcc GKPIVGATGREMPVS IGHV3-23 IGHD1-26 IGHJ4 ggattcacctttagcaactatgcc atgaactgggtccgccaggttccaggggaggggctggagtgggtctcagcc attagtggcagtggtggtagcaca ttctacacagacgccttgcagggccgattcaccatctccagagacaattccaagaacacgttatatttgcaaatgaaaagcctgagagccggggacacggccgtgtattactgt gggaagccaatagtgggcgcaacaggtagagagatgcctgtttcc unmatched 18 213 13 5 8 10 5 8 6 1 10 1 JY8QFUQ01CF06T 7655 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 977 94.5 206/218 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD4-17*01 F 2 8 8 11 8.8.11 [1.17.38.11] CAGHLLGDPFDYW in-frame + gttctggagacaactttagcagatactggatcggctgggtccgccagatgcccgggaaaggcctggagtggatggggatcatctatcctggtgactctgacaccagatacagtccgtccttccaaggccaggtcaccatctcagccgacaagtccaccagtaccgcctacctgcagtggagcagtctgaaggtctcggacaccgccacgtattactgtgcgggacatcttctcggtgacccttttgactactggggccagggaaccccggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggcaactcag NA unmatched, IGG1 64 88 [283] t82>g,Y28>D(- - -)|g86>a,S29>N(- - -)|c104>g,T35>S(+ - +)|c108>a,S36>R(- - -)|g126>c|t192>c|c204>t|t251>c,I84>T(- - -)|c255>t|c279>t|c287>t,A96>V(+ - +)|t302>c,M101>T(- - -)|a316>g,R106>G(- - -)| t82>g,Y28>D(- - -)|g86>a,S29>N(- - -)|c104>g,T35>S(+ - +)|c108>a,S36>R(- - -)| g126>c| t192>c| c204>t|t251>c,I84>T(- - -)|c255>t|c279>t|c287>t,A96>V(+ - +)|t302>c,M101>T(- - -)| a316>g,R106>G(- - -)| 320 226 213 13 5 8 1 1 4 3 0 1 0 0 1 1 0 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 1 0 0 0 1 0 0 0 1 0 1 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 23 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 117 114 108 6 3 3 0 0 4 2 0 0 0 0 0 0 0 0 8 8 7 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| gttct ggagacaactttagcagatactgg atcggctgggtccgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgacacc agatacagtccgtccttccaaggccaggtcaccatctcagccgacaagtccaccagtaccgcctacctgcagtggagcagtctgaaggtctcggacaccgccacgtattactgt gcgggacatcttctcggtgacccttttgactac AGHLLGDPFDY IGHV5-51 IGHD4-17 IGHJ4 ggagacaactttagcagatactgg atcggctgggtccgccagatgcccgggaaaggcctggagtggatggggatc atctatcctggtgactctgacacc agatacagtccgtccttccaaggccaggtcaccatctcagccgacaagtccaccagtaccgcctacctgcagtggagcagtctgaaggtctcggacaccgccacgtattactgt gcgggacatcttctcggtgacccttttgactac unmatched 12 213 8 4 5 8 3 4 4 1 3 4 JY8QFUQ01CG8U2 15783 productive (see comment) Homsap IGHV3-66*02 F 908 91.63 197/215 nt 91.16 196/215 nt Homsap IGHJ4*02 F (see comment) 186 87.5 42/48 nt Homsap IGHD1-26*01 F 1 8 7 13 8.7.13 [1.17.38.11] CASVGVGATVAFQYW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ1*01 (highest number of consecutive identical nucleotides) in CDR2-IMGT at codon 58 of V-REGION: 'G' (1 nucleotides at position 88 to 88 in the user submitted sequence), (cause frameshift); cctctggattcaccgtcagtgatagttacatgagctgggtccgccaggctccagggaaggggctggagtgggtctcgattatttataGggggaggtaccacatattatgccgactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctatatcttcaaatgaacaccctgagaggtgaggacacggctctatattactgtgcgagcgtcggagtgggagctacagtcgcctttcaatactggggccagggcaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccgaagatgggaacgtggtcgtcgcctgcctggtccagggcttcttcccccaa NA unmatched, IGA2 57 95 [286, 288] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|a110>g,N37>S(- - -)|c111>t,N37>S(- - -)|a162>g|g163>a,V55>I(+ - +)|c174>g,S58>R(- - -)|t177>a|g191>c,S64>T(+ - +)|c198>t|c201>t|a204>c|g261>a|g278>c,S93>T(+ - +)|c287>g,A96>G(- + -)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|c108>t,S36>D(- - -)|a110>g,N37>S(- - -)|c111>t,N37>S(- - -)| a162>g|g163>a,V55>I(+ - +)| c174>g,S58>R(- - -)|t177>a|g191>c,S64>T(+ - +)| c198>t|c201>t|a204>c|g261>a|g278>c,S93>T(+ - +)|c287>g,A96>G(- + -)|g301>c,V101>L(+ - +)|g303>a,V101>L(+ - +)| a318>c,R106>S(- - -)| 317 (318) 220 (221) 202 18 (19) 6 12 (13) 3 4 4 0 1 (2) 0 0 1 3 2 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 2 1 2 0 0 0 0 0 0 0 0 0 51 51 49 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 30 21 18 3 1 2 0 0 0 0 0 0 0 1 1 1 0 0 117 114 106 8 4 4 0 2 2 0 1 0 0 0 2 1 0 0 5 (6) 5 5 0 (1) 0 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccgtcagtgatagttac atgagctgggtccgccaggctccagggaaggggctggagtgggtctcgatt atttataggggaggtaccaca tattatgccgactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctatatcttcaaatgaacaccctgagaggtgaggacacggctctatattactgt gcgagcgtcggagtgggagctacagtcgcctttcaatac ASVGVGATVAFQY IGHV3-66 IGHD1-26 IGHJ4 ggattcaccgtcagtgatagttac atgagctgggtccgccaggctccagggaaggggctggagtgggtctcgatt atttataggggaggtaccaca tattatgccgactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctatatcttcaaatgaacaccctgagaggtgaggacacggctctatattactgt gcgagcgtcggagtgggagctacagtcgcctttcaatac unmatched 18 210 11 7 8 13 3 5 5 1 5 7 JY8QFUQ01CGLTX 21930 productive (see comment) Homsap IGHV3-49*03 F, or Homsap IGHV3-49*05 F 967 92.44 208/225 nt 92 207/225 nt Homsap IGHJ3*01 F 232 96 48/50 nt Homsap IGHD1-26*01 F 3 8 10 14 8.10.14 [2.17.38.11] CTRDLSGSYSGAFDVW in-frame + Potentially, because of detected insertion/deletion in FR3-IMGT at codon 84 of V-REGION: 'A' (1 nucleotides at position 163 to 163 in the user submitted sequence), (cause frameshift); ggatctggattcacctttggtgactttgctatgagctggtttcgccaggctccagggaaggggctggagtggctaggtttcattagaagcaaaatttatggtgggacaccagaatacgccgcgtctgtgaaaggcagatgtcccatctcaagagatgattccAaaaaacatcgcctatctgcaaataaacggcctgaaaaccgaggacacagccatgtatttctgtaccagagatctcagtgggagctactccggtgcttttgatgtctggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccagcagatgggaacgtggtcatcgcctgcctcgtccaggctcttccccca NA unmatched, IGA1 64 89 [299, 298] c74>g,A25>G(- + -)|t75>a,A25>G(- + -)|t108>c|a110>t,Y37>F(- + -)|c126>t|g157>c,V53>L(+ - +)|g178>a,A60>I(+ - +)|c179>t,A60>I(+ - +)|a193>c,T65>P(+ + -)|t227>g,F76>C(+ - -)|c228>t,F76>C(+ - -)|a229>c,T77>P(+ + -)|g254>a,S85>N(- - -)|g273>a,M91>I(+ + -)|a277>g,S93>G(+ + -)|g301>a,V101>M(+ - -)|a308>t,Y103>F(- + -)|t315>c| c74>g,A25>G(- + -)|t75>a,A25>G(- + -)| t108>c|a110>t,Y37>F(- + -)| c126>t|g157>c,V53>L(+ - +)| g178>a,A60>I(+ - +)|c179>t,A60>I(+ - +)|a193>c,T65>P(+ + -)| t227>g,F76>C(+ - -)|c228>t,F76>C(+ - -)|a229>c,T77>P(+ + -)|g254>a,S85>N(- - -)|g273>a,M91>I(+ + -)|a277>g,S93>G(+ + -)|g301>a,V101>M(+ - -)|a308>t,Y103>F(- + -)| t315>c| 320 233 215 18 3 15 1 4 3 2 2 0 2 1 1 1 0 1 78 6 4 2 0 2 0 0 0 0 0 0 0 1 0 1 0 0 36 24 22 2 1 1 0 0 0 1 0 0 1 0 0 0 0 0 51 51 49 2 1 1 0 0 1 0 0 0 0 0 1 0 0 0 30 30 27 3 0 3 0 1 1 0 1 0 0 0 0 0 0 0 117 114 106 8 0 8 1 3 1 0 1 0 1 0 0 0 0 1 8 8 7 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,69-70(FR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,158-159(FR2)|ta,168-169(CDR2)|aa,171-172(CDR2)|aa,175-176(CDR2)|ta,181-182(CDR2)|aa,192-193(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,250-251(FR3)|aa,252-253(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| tt,34-35(FR1)|ta,38-39(FR1)|ta,69-70(FR1)|tt,75-76(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|tt,108-109(CDR1)|ta,114-115(CDR1)|tt,124-125(FR2)|ta,158-159(FR2)|tt,162-163(FR2)|tt,167-168(CDR2)|tt,180-181(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)|ta,315-316(CDR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agct,72-75(FR1)|agct,118-121(FR2)|ggtt,122-125(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggta,156-159(FR2)|ggtt,160-163(FR2)|agca,172-175(CDR2)|agct,177-180(CDR2)|ggca,220-223(FR3)|agca,253-256(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,72-75(FR1)|tgct,111-114(CDR1)|agct,118-121(FR2)|agct,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tact,312-315(FR3)| ggatct ggattcacctttggtgactttgct atgagctggtttcgccaggctccagggaaggggctggagtggctaggtttc attagaagcaaaatttatggtgggacacca gaatacgccgcgtctgtgaaaggcagatgtcccatctcaagagatgattccaaaaacatcgcctatctgcaaataaacggcctgaaaaccgaggacacagccatgtatttctgt accagagatctcagtgggagctactccggtgcttttgatgtc TRDLSGSYSGAFDV IGHV3-49 IGHD1-26 IGHJ3 ggattcacctttggtgactttgct atgagctggtttcgccaggctccagggaaggggctggagtggctaggtttc attagaagcaaaatttatggtgggacacca gaatacgccgcgtctgtgaaaggcagatgtcccatctcaagagatgattccaaaaacatcgcctatctgcaaataaacggcctgaaaaccgaggacacagccatgtatttctgt accagagatctcagtgggagctactccggtgcttttgatgtc unmatched 15 219 9 6 7 8 2 7 1 1 9 4 JY8QFUQ01CGQFF 3373 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 891 90.32 196/217 nt NA Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD3-10*01 F 1 8 8 14 8.8.14 [1.17.38.11] CVRQLGFGDLFRFDPW in-frame + ctctggatacagttttaacagttatgccatgacttgggtccgccaggctccagggaaggggctggagtgggtctcaactattagtggcactggtggtaaccaatactacgcagactccgtgaggggccggctcaccatctccagagacaattccaagaacacactatttctgcagatgagcagcctgagagccgaggacacggccgtttattactgtgtgagacaattgggattcggggacttattccggttcgacccctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccctaagcatgggaacgtggtcatcgcctgcctggtccagggcttcttccccca NA unmatched, IGA1 29 84 [290, 292] t83>a,F28>Y(- + -)|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>a,S35>N(- - -)|c108>t|g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g163>a,A55>T(- - -)|t174>c|g176>c,S59>T(+ - +)|g191>a,S64>N(- - -)|a193>c,T65>Q(- - -)|c194>a,T65>Q(- - -)|a215>g,K72>R(+ + +)|t226>c,F76>L(+ - -)|g258>a|g261>a|a263>t,Y88>F(- + -)|a270>g|a275>g,N92>S(- - -)|a303>t|c314>t,A105>V(+ - +)| t83>a,F28>Y(- + -)|c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>a,S35>N(- - -)|c108>t| g119>c,S40>T(+ - +)|c120>t,S40>T(+ - +)|g163>a,A55>T(- - -)| t174>c|g176>c,S59>T(+ - +)|g191>a,S64>N(- - -)|a193>c,T65>Q(- - -)|c194>a,T65>Q(- - -)| a215>g,K72>R(+ + +)|t226>c,F76>L(+ - -)|g258>a|g261>a|a263>t,Y88>F(- + -)|a270>g|a275>g,N92>S(- - -)|a303>t| c314>t,A105>V(+ - +)|a317>g,K106>R(+ + +)|g319>c| 316 (320) 221 (225) 199 (201) 22 (24) 6 (7) 16 (17) 3 (4) 5 4 2 1 1 2 1 2 (3) 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 1 2 0 0 0 0 1 0 1 0 0 51 51 48 3 0 3 0 1 1 0 0 0 0 0 1 0 0 0 30 24 19 5 1 4 0 1 0 1 1 1 0 0 1 0 0 0 117 114 106 8 4 4 3 2 0 1 0 0 2 0 0 0 0 0 4 (8) 4 3 (5) 1 (3) 0 (1) 1 (2) 0 (1) 0 1 0 0 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatacagttttaacagttatgcc atgacttgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtggcactggtggtaaccaa tactacgcagactccgtgaggggccggctcaccatctccagagacaattccaagaacacactatttctgcagatgagcagcctgagagccgaggacacggccgtttattactgt gtgagacaattgggattcggggacttattccggttcgacccc VRQLGFGDLFRFDP IGHV3-23 IGHD3-10 IGHJ5 ggatacagttttaacagttatgcc atgacttgggtccgccaggctccagggaaggggctggagtgggtctcaact attagtggcactggtggtaaccaa tactacgcagactccgtgaggggccggctcaccatctccagagacaattccaagaacacactatttctgcagatgagcagcctgagagccgaggacacggccgtttattactgt gtgagacaattgggattcggggacttattccggttcgacccc unmatched 21 213 13 8 8 12 5 9 4 2 7 8 JY8QFUQ01CHDDF 19489 productive (see comment) Homsap IGHV3-15*01 F, or Homsap IGHV3-15*02 F 899 89.29 200/224 nt 87.95 197/224 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-6*01 F 2 8 10 12 8.10.12 [1.17.38.11] CTITVRPDDYHDYW in-frame + Potentially, because of detected insertion/deletion in FR2-IMGT at codon 53 of V-REGION: 'G' (1 nucleotides at position 72 to 72 in the user submitted sequence), (cause frameshift);in CDR2-IMGT at codon 57 of V-REGION: 'A' (1 nucleotides at position 87 to 87 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 85 of V-REGION: 'T' (1 nucleotides at position 169 to 169 in the user submitted sequence), (cause frameshift); tctctggagtcactttcactaacgtgtggatgagttgggtccggcaggctccagggaaggggccggagtggGgttggccgtattaaAaagggagactgagggggggacaatagactacgctgcacccgtgacagcaagattcaccatgtcaaaagatgattcaaaaaaTcacactatatctgcaaatgaacaacctgaaaatggaggacacagccgtgtattactgtaccataacagtccgaccggatgattaccatgactactggggccagggaaccctggtgaccgtctccccagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggctccagg NA unmatched, IGA2 57 89 [294, 295] c74>t|t82>g,F28>V(+ - -)|g104>c,S35>T(+ - +)|c110>t,A37>V(+ - +)|c111>g,A37>V(+ - +)|c120>t|c129>g|t149>c,L50>P(- - -)|c174>g,S58>R(- - -)|a175>g,K59>E(+ - -)|a177>g,K59>E(+ - -)|t183>g,D61>E(+ - +)|t186>g|c194>t,T65>I(- - -)|a215>c,K72>T(- - -)|g221>c,G74>A(- + -)|c222>a,G74>A(- + -)|c234>g,I78>M(+ + -)|g239>a,R80>K(+ + +)|g258>a|g261>a|g278>a,S93>N(- - -)|c287>t,T96>M(- - -)|c288>g,T96>M(- - -)| c74>t| t82>g,F28>V(+ - -)|g104>c,S35>T(+ - +)|c110>t,A37>V(+ - +)|c111>g,A37>V(+ - +)| c120>t|c129>g|t149>c,L50>P(- - -)| c174>g,S58>R(- - -)|a175>g,K59>E(+ - -)|a177>g,K59>E(+ - -)|t183>g,D61>E(+ - +)|t186>g|c194>t,T65>I(- - -)| a215>c,K72>T(- - -)|g221>c,G74>A(- + -)|c222>a,G74>A(- + -)|c234>g,I78>M(+ + -)|g239>a,R80>K(+ + +)|g258>a|g261>a|g278>a,S93>N(- - -)|c287>t,T96>M(- - -)|c288>g,T96>M(- - -)| c317>t,T106>I(- - -)|g319>a|a320>c| 316 (320) 228 (232) 204 (205) 24 (27) 6 (8) 18 (19) 2 4 (5) 5 (6) 1 1 (2) 1 0 0 2 5 0 3 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 1 0 0 0 0 0 1 1 0 1 51 51 48 3 2 1 0 0 1 1 0 0 0 0 0 1 0 0 30 30 24 6 1 5 2 0 1 0 0 0 0 0 0 1 0 2 117 114 104 10 2 8 0 4 1 0 1 1 0 0 1 2 0 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 0 (1) 0 (1) 0 0 (1) 0 0 0 0 0 0 0 ta,38-39(FR1)|aa,40-41(FR1)|ta,57-58(FR1)|ta,105-106(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|aa,170-171(CDR2)|aa,175-176(CDR2)|aa,177-178(CDR2)|aa,192-193(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,237-238(FR3)|aa,249-250(FR3)|aa,251-252(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|aa,283-284(FR3)|aa,285-286(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|ta,312-313(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,56-57(FR1)|tt,82-83(CDR1)|tt,87-88(CDR1)|ta,105-106(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)|ta,312-313(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agca,172-175(CDR2)|ggca,220-223(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,118-121(FR2)|aact,177-180(CDR2)|agcc,277-280(FR3)|aacc,285-288(FR3)|agcc,297-300(FR3)|tact,307-310(FR3)|tacc,312-315(FR3)| tctct ggagtcactttcactaacgtgtgg atgagttgggtccggcaggctccagggaaggggccggagtgggttggccgt attaaaagggagactgagggggggacaata gactacgctgcacccgtgacagcaagattcaccatgtcaaaagatgattcaaaaaacacactatatctgcaaatgaacaacctgaaaatggaggacacagccgtgtattactgt accataacagtccgaccggatgattaccatgactac TITVRPDDYHDY IGHV3-15 IGHD6-6 IGHJ4 ggagtcactttcactaacgtgtgg atgagttgggtccggcaggctccagggaaggggccggagtgggttggccgt attaaaagggagactgagggggggacaata gactacgctgcacccgtgacagcaagattcaccatgtcaaaagatgattcaaaaaacacactatatctgcaaatgaacaacctgaaaatggaggacacagccgtgtattactgt accataacagtccgaccggatgattaccatgactac unmatched 23 219 11 12 8 16 3 7 4 1 9 9 JY8QFUQ01CHKLB 16344 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 1008 96.31 209/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD3-22*01 F 2 8 8 15 8.8.15 [1.17.38.11] CILLYYDSRGVFSTDYW in-frame + ctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggcgtgggtctcacgtattaaaagtgatggcagtggcacaaactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagagcacgctgtttctgcaaatgaacagtctgagagccgaggacacggctgtatattactgtatactgctttactatgatagtagaggtgtcttttcaaccgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacacgcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggctt NA unmatched, IGA2 64 84 [293] t152>c,V51>A(+ - +)|t171>a,N57>K(+ - -)|g186>c|a190>g,S64>G(+ + -)|g197>a,S66>N(- - -)|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|g303>a| t152>c,V51>A(+ - +)| t171>a,N57>K(+ - -)|g186>c|a190>g,S64>G(+ + -)| g197>a,S66>N(- - -)|a254>g,N85>S(- - -)|a263>t,Y88>F(- + -)|g303>a| g313>a,A105>I(+ - +)|c314>t,A105>I(+ - +)|a316>c,R106>L(- + -)|g317>t,R106>L(- + -)|a318>g,R106>L(- + -)|g319>c|a320>t| 312 (320) 217 (225) 209 (210) 8 (15) 2 (4) 6 (11) 2 (3) 2 (3) 0 (1) 1 0 (1) 0 1 (2) 1 1 (2) 0 0 (1) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 30 24 21 3 1 2 1 0 0 0 0 0 0 1 1 0 0 0 117 114 110 4 1 3 1 2 0 0 0 0 1 0 0 0 0 0 0 (8) 0 0 (1) 0 (7) 0 (2) 0 (5) 0 (1) 0 (1) 0 (1) 0 0 (1) 0 0 (1) 0 0 (1) 0 0 (1) 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggcgtgggtctcacgt attaaaagtgatggcagtggcaca aactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagagcacgctgtttctgcaaatgaacagtctgagagccgaggacacggctgtatattactgt atactgctttactatgatagtagaggtgtcttttcaaccgactac ILLYYDSRGVFSTDY IGHV3-74 IGHD3-22 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggcgtgggtctcacgt attaaaagtgatggcagtggcaca aactacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagagcacgctgtttctgcaaatgaacagtctgagagccgaggacacggctgtatattactgt atactgctttactatgatagtagaggtgtcttttcaaccgactac unmatched 8 213 5 3 2 3 3 5 1 1 4 2 JY8QFUQ01CHW93 15801 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 950 93.12 203/218 nt NA Homsap IGHJ4*02 F (see comment) 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 8 19 8.8.19 [1.17.38.11] CAKDGQRYCSNGVCSSFFDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) tgtctggattcacatttagaagctattccatgaattgggtccgccaggctccagggaaggggctggagtgggtctcagcaattagtggtggtggtgctagtacataccacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatttgcaaatgaacagcctgagagccgacgacacggccgtatattactgtgcgaaagatggacaacgatattgctctaatggtgtatgctcgtccttctttgactcctggggccagggaaccctggtcaccgtctcctcagcctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca NA unmatched, IGA1 0 0 [] c74>t|c75>g|c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|t165>a|a175>g,S59>G(+ + -)|g188>c,G63>A(- + -)|c192>t|t199>c,Y67>H(+ - -)|g225>a|c265>t|g291>c,E97>D(+ - +)| c74>t|c75>g| c87>a|c105>a,S35>R(- - -)|g112>t,A38>S(- + -)| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|t165>a| a175>g,S59>G(+ + -)|g188>c,G63>A(- + -)|c192>t| t199>c,Y67>H(+ - -)|g225>a|c265>t|g291>c,E97>D(+ - +)| 320 226 211 15 7 8 1 2 4 1 0 2 0 1 2 1 1 0 78 5 3 2 2 0 0 0 1 0 0 0 0 0 0 1 0 0 36 24 21 3 1 2 0 0 0 0 0 2 0 0 0 0 1 0 51 51 48 3 1 2 0 1 1 0 0 0 0 1 0 0 0 0 30 24 21 3 1 2 1 0 1 0 0 0 0 0 1 0 0 0 117 114 110 4 2 2 0 1 1 1 0 0 0 0 1 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tgtct ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagca attagtggtggtggtgctagtaca taccacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatttgcaaatgaacagcctgagagccgacgacacggccgtatattactgt gcgaaagatggacaacgatattgctctaatggtgtatgctcgtccttctttgactcc AKDGQRYCSNGVCSSFFDS IGHV3-23 IGHD2-8 IGHJ4 ggattcacatttagaagctattcc atgaattgggtccgccaggctccagggaaggggctggagtgggtctcagca attagtggtggtggtgctagtaca taccacgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatttgcaaatgaacagcctgagagccgacgacacggccgtatattactgt gcgaaagatggacaacgatattgctctaatggtgtatgctcgtccttctttgactcc unmatched 13 213 7 6 5 10 2 3 3 2 4 4 JY8QFUQ01CII5W 15315 productive Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F (see comment) 773 84.65 182/215 nt NA Homsap IGHJ6*02 F (see comment) 175 75.81 47/62 nt Homsap IGHD3-10*01 F 2 8 7 14 8.7.14 [1.17.38.11] CARERGSGPPTGMDVW in-frame + Low V-REGION identity (84,65% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) or Homsap_IGHJ4*03 (shorter alignment but highest percentage of identity) gttatgggttgaccgtcggtgccgaccacatgtactgggtccgccaggctccagggaaggggctgaagtgggtctcagttctttatggcggtggcaccttggactacgcagactccgtgaagggccgattcaccatctccagagacaactcgaggaacactgtgtatcttcagatggagagactgagccccgaggacacggccgtctactactgtgcgagagaaagaggttcgggcccccccacaggcatggacgtctggggcccagggaccatggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG1 64 88 [289] c74>g|c75>t|c77>a,S26>Y(+ - -)|c84>g,F28>L(+ - -)|a103>g,S35>G(+ + -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a109>g,N37>D(+ + -)|t112>c,Y38>H(+ - -)|a118>t,S40>Y(+ - -)|g119>a,S40>Y(+ - -)|g151>a,E51>K(+ - -)|a166>c,I56>L(+ + +)|a172>g,S58>G(+ + -)|t189>c|g191>c,S64>T(+ - +)|a193>t,T65>L(- - -)|c194>t,T65>L(- - -)|a195>g,T65>L(- - -)|t196>g,Y66>D(- - -)|t246>c|c249>g|a251>g,K84>R(+ + +)|g258>t|c259>g,L87>V(+ - +)|a270>g|a274>g,N92>E(+ - -)|c276>g,N92>E(+ - -)|c279>a,S93>R(- - -)|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|g303>c|t306>c| c74>g|c75>t|c77>a,S26>Y(+ - -)| c84>g,F28>L(+ - -)|a103>g,S35>G(+ + -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|a109>g,N37>D(+ + -)|t112>c,Y38>H(+ - -)| a118>t,S40>Y(+ - -)|g119>a,S40>Y(+ - -)|g151>a,E51>K(+ - -)| a166>c,I56>L(+ + +)|a172>g,S58>G(+ + -)|t189>c|g191>c,S64>T(+ - +)|a193>t,T65>L(- - -)|c194>t,T65>L(- - -)|a195>g,T65>L(- - -)| t196>g,Y66>D(- - -)|t246>c|c249>g|a251>g,K84>R(+ + +)|g258>t|c259>g,L87>V(+ - +)|a270>g|a274>g,N92>E(+ - -)|c276>g,N92>E(+ - -)|c279>a,S93>R(- - -)|a285>c,R95>S(- - -)|g286>c,A96>P(- - -)|g303>c|t306>c| 320 223 190 33 9 24 8 2 2 4 2 2 2 0 4 5 1 1 78 5 2 3 2 1 0 0 1 0 0 1 0 0 0 1 0 0 36 24 18 6 0 6 3 0 0 1 0 0 0 0 1 1 0 0 51 51 48 3 0 3 0 2 0 0 0 0 1 0 0 0 0 0 30 21 14 7 1 6 2 0 1 1 1 0 1 0 1 0 0 0 117 114 100 14 6 8 3 0 0 2 1 1 0 0 2 3 1 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gttat gggttgaccgtcggtgccgaccac atgtactgggtccgccaggctccagggaaggggctgaagtgggtctcagtt ctttatggcggtggcaccttg gactacgcagactccgtgaagggccgattcaccatctccagagacaactcgaggaacactgtgtatcttcagatggagagactgagccccgaggacacggccgtctactactgt gcgagagaaagaggttcgggcccccccacaggcatggacgtc ARERGSGPPTGMDV IGHV3-53 IGHD3-10 IGHJ6 gggttgaccgtcggtgccgaccac atgtactgggtccgccaggctccagggaaggggctgaagtgggtctcagtt ctttatggcggtggcaccttg gactacgcagactccgtgaagggccgattcaccatctccagagacaactcgaggaacactgtgtatcttcagatggagagactgagccccgaggacacggccgtctactactgt gcgagagaaagaggttcgggcccccccacaggcatggacgtc unmatched 30 210 15 15 3 13 12 17 6 1 11 12 JY8QFUQ01CINZT 21162 productive Homsap IGHV3-7*01 F 1035 97.7 212/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD4-23*01 ORF 2 8 8 12 8.8.12 [1.17.38.11] CVSSPYFYDSNGYW in-frame + ctctggattcaggtttagcatctattggatgagctgggtccgccaggctccagggaaggggctggagtgggtggccaacataaagcaagatggaagtgagaaatactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagcagcctgagagccgaggacacggctgtgtattactgtgtgagttcaccgtatttctatgatagtaatggttactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacacgcacccccaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 64 84 [284] c86>g,T29>R(- - -)|c87>g,T29>R(- - -)|t105>c|g107>t,S36>I(- - -)|a275>g,N92>S(- - -)|c314>t,A105>V(+ - +)| c86>g,T29>R(- - -)|c87>g,T29>R(- - -)|t105>c|g107>t,S36>I(- - -)| a275>g,N92>S(- - -)| c314>t,A105>V(+ - +)|a318>t,R106>S(- - -)|g319>t|a320>c| 317 (320) 222 (225) 216 6 (9) 1 (3) 5 (6) 1 0 1 1 0 (1) 0 0 (1) 0 0 2 1 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 0 1 0 0 0 0 0 2 1 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 113 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 5 (8) 5 4 1 (4) 0 (2) 1 (2) 0 0 1 0 0 (1) 0 0 (1) 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaggtttagcatctattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaagtgagaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagcagcctgagagccgaggacacggctgtgtattactgt gtgagttcaccgtatttctatgatagtaatggttac VSSPYFYDSNGY IGHV3-7 IGHD4-23 IGHJ4 ggattcaggtttagcatctattgg atgagctgggtccgccaggctccagggaaggggctggagtgggtggccaac ataaagcaagatggaagtgagaaa tactatgtggactctgtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgagcagcctgagagccgaggacacggctgtgtattactgt gtgagttcaccgtatttctatgatagtaatggttac unmatched 5 213 2 3 0 3 2 2 0 1 1 3 JY8QFUQ01CJLXK 7439 productive Homsap IGHV3-33*01 F, or Homsap IGHV3-33*06 F 882 89.86 195/217 nt NA Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD6-19*01 F 1 8 8 13 8.8.13 [1.17.38.11] CARDPSGSGWAFESW in-frame + gtctggattccctttcagtagagatgccatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatggtatggtatgatggaagtaatacacaccatgcagattccgtgaagggccgattcatcatttccagagacaattccaagaataaagtgtatctgcaaatgaacagtctgagagacgaggacacggctgtctattattgtgcgagagatccgagcggcagtggctgggcctttgagtcctggggccagggagccctggtcaccgtctcctcagcatccccgaccagcccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 84 [287, 286] a85>c,T29>P(+ + -)|c87>t,T29>P(+ + -)|c108>a,S36>R(- - -)|t109>g,Y37>D(- - -)|g113>c,G38>A(- + -)|g163>a,V55>M(+ - -)|t165>g,V55>M(+ - -)|a166>g,I56>V(+ - +)|a194>c,K65>T(- - -)|t196>c,Y66>H(+ - -)|t199>c,Y67>H(+ - -)|c207>t|c230>t,T77>I(- - -)|c234>t|c255>t|c257>a,T86>K(- - -)|g258>a,T86>K(- - -)|c259>g,L87>V(+ - +)|c279>t|c287>a,A96>D(- - -)|g303>c|c309>t| a85>c,T29>P(+ + -)|c87>t,T29>P(+ + -)|c108>a,S36>R(- - -)|t109>g,Y37>D(- - -)|g113>c,G38>A(- + -)| g163>a,V55>M(+ - -)|t165>g,V55>M(+ - -)| a166>g,I56>V(+ - +)|a194>c,K65>T(- - -)| t196>c,Y66>H(+ - -)|t199>c,Y67>H(+ - -)|c207>t|c230>t,T77>I(- - -)|c234>t|c255>t|c257>a,T86>K(- - -)|g258>a,T86>K(- - -)|c259>g,L87>V(+ - +)|c279>t|c287>a,A96>D(- - -)|g303>c|c309>t| 320 225 203 22 6 16 1 2 7 2 2 3 0 0 2 1 0 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 0 0 1 0 1 1 0 0 1 0 0 1 51 51 49 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 30 24 22 2 0 2 1 0 0 0 1 0 0 0 0 0 0 0 117 114 101 13 6 7 0 1 6 2 0 2 0 0 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|ggta,170-173(CDR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| gtct ggattccctttcagtagagatgcc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatg gtatggtatgatggaagtaataca caccatgcagattccgtgaagggccgattcatcatttccagagacaattccaagaataaagtgtatctgcaaatgaacagtctgagagacgaggacacggctgtctattattgt gcgagagatccgagcggcagtggctgggcctttgagtcc ARDPSGSGWAFES IGHV3-33 IGHD6-19 IGHJ4 ggattccctttcagtagagatgcc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatg gtatggtatgatggaagtaataca caccatgcagattccgtgaagggccgattcatcatttccagagacaattccaagaataaagtgtatctgcaaatgaacagtctgagagacgaggacacggctgtctattattgt gcgagagatccgagcggcagtggctgggcctttgagtcc unmatched 22 213 12 10 9 15 3 7 6 0 9 7 JY8QFUQ01CJT9B 3792 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 801 85.71 186/217 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD1-26*01 F 1 8 8 13 8.8.13 [1.17.38.11] CAKDAVGLTRLFGDW in-frame + ctctggatttgtctttagtagatatgccatggcctgggtccgccaggctccagggcaggggctggagtgggtcgccagtattggcgggagtggtgataacacatactacgcggactccgtgaagggccggttcaccatctccagagacaactccaataacaaactgtttctgcaaatggacagtttgcgagccggggacacggccagatatttctgtgcgaaagatgcggtgggacttacccgactctttggtgactggggccagggaaccgtggtcagcgccgcctctgcatccccgacagccccaaaggtcttcccgctgagcctcgacagcacccccctcaaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA unmatched, IGA2 57 68 [287, 290] c84>t|a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|c105>t|c108>a,S36>R(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a142>c,K48>Q(+ - -)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|a169>g,S57>G(+ + -)|t171>c,S57>G(+ + -)|t174>g|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)|a204>g|t246>c|g252>t,K84>N(+ - -)|c257>a,T86>K(- - -)|g258>a,T86>K(- - -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|c280>t|a283>c|a290>g,E97>G(- - -)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)|a308>t,Y103>F(- + -)| c84>t|a85>g,T29>V(- - -)|c86>t,T29>V(- - -)|c105>t|c108>a,S36>R(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a142>c,K48>Q(+ - -)|t160>g,S54>A(- + -)|a162>c,S54>A(- + -)|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| a169>g,S57>G(+ + -)|t171>c,S57>G(+ + -)|t174>g|g188>a,G63>D(- - -)|g191>a,S64>N(- - -)| a204>g|t246>c|g252>t,K84>N(+ - -)|c257>a,T86>K(- - -)|g258>a,T86>K(- - -)|a263>t,Y88>F(- + -)|a274>g,N92>D(+ + -)|c279>t|c280>t|a283>c|a290>g,E97>G(- - -)|g301>a,V101>R(- - -)|t302>g,V101>R(- - -)|a308>t,Y103>F(- + -)| 320 225 194 31 8 23 6 5 5 2 3 2 2 0 1 1 1 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 2 3 1 0 3 0 0 1 0 0 0 0 0 0 51 51 44 7 0 7 1 1 0 0 2 0 0 0 1 1 0 1 30 24 19 5 1 4 1 2 0 1 0 0 0 0 0 0 0 1 117 114 100 14 5 9 3 2 2 1 1 1 2 0 0 0 1 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggatttgtctttagtagatatgcc atggcctgggtccgccaggctccagggcaggggctggagtgggtcgccagt attggcgggagtggtgataacaca tactacgcggactccgtgaagggccggttcaccatctccagagacaactccaataacaaactgtttctgcaaatggacagtttgcgagccggggacacggccagatatttctgt gcgaaagatgcggtgggacttacccgactctttggtgac AKDAVGLTRLFGD IGHV3-23 IGHD1-26 IGHJ4 ggatttgtctttagtagatatgcc atggcctgggtccgccaggctccagggcaggggctggagtgggtcgccagt attggcgggagtggtgataacaca tactacgcggactccgtgaagggccggttcaccatctccagagacaactccaataacaaactgtttctgcaaatggacagtttgcgagccggggacacggccagatatttctgt gcgaaagatgcggtgggacttacccgactctttggtgac unmatched 31 213 18 13 10 15 8 16 5 3 16 7 JY8QFUQ01CK1VY 9216 productive Homsap IGHV4-61*01 F, or Homsap IGHV4-61*03 F 848 87.33 193/221 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD6-19*01 F 1 10 7 12 10.7.12 [1.17.38.11] CARGGGWTPYFDSW in-frame + tctctggtggctccgtcagcagtggtaattactactggaactggatccgccaacccccagggaagggactggagtggattggatatatctactatgctggggccaccaacgtcgccccctccctcaagaaccgagtcaccataacgagagacacgtccaagaaccaattttccctgaggttgacttctgtgaccgctgcggacacggccgtatattactgtgcgaggggcggtggctggaccccctactttgactcctggggccagggaaatctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccccctaagatcgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA1 29 84 [288, 290] g107>a,S36>N(- - -)|g119>a,S40>N(- - -)|g129>c|g132>a|g162>a|t171>c|c174>t|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|t199>g,Y67>V(- - -)|a200>t,Y67>V(- - -)|a202>g,N68>A(- - -)|a203>c,N68>A(- - -)|g221>a,S74>N(- - -)|t222>c,S74>N(- - -)|t235>a,S79>T(+ - +)|a237>g,S79>T(+ - +)|g238>a,V80>R(- - -)|t239>g,V80>R(- - -)|g258>a|c261>t|a269>g,K90>R(+ + +)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g303>a| g107>a,S36>N(- - -)| g119>a,S40>N(- - -)|g129>c|g132>a|g162>a| t171>c|c174>t|a175>g,S59>A(- + -)|g176>c,S59>A(- + -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| t199>g,Y67>V(- - -)|a200>t,Y67>V(- - -)|a202>g,N68>A(- - -)|a203>c,N68>A(- - -)|g221>a,S74>N(- - -)|t222>c,S74>N(- - -)|t235>a,S79>T(+ - +)|a237>g,S79>T(+ - +)|g238>a,V80>R(- - -)|t239>g,V80>R(- - -)|g258>a|c261>t|a269>g,K90>R(+ + +)|c271>t|g275>c,S92>T(+ - +)|c276>t,S92>T(+ - +)|g303>a| a318>g|a320>g| 317 (320) 226 (229) 198 (199) 28 (30) 9 (11) 19 5 (7) 8 4 2 1 0 1 1 4 0 0 2 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 29 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 51 51 47 4 3 1 0 3 0 0 0 0 0 0 1 0 0 0 30 21 15 6 2 4 2 0 1 1 0 0 0 0 2 0 0 0 117 114 97 17 4 13 3 4 3 1 1 0 1 1 1 0 0 2 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcagcagtggtaattactac tggaactggatccgccaacccccagggaagggactggagtggattggatat atctactatgctggggccacc aacgtcgccccctccctcaagaaccgagtcaccataacgagagacacgtccaagaaccaattttccctgaggttgacttctgtgaccgctgcggacacggccgtatattactgt gcgaggggcggtggctggaccccctactttgactcc ARGGGWTPYFDS IGHV4-61 IGHD6-19 IGHJ4 ggtggctccgtcagcagtggtaattactac tggaactggatccgccaacccccagggaagggactggagtggattggatat atctactatgctggggccacc aacgtcgccccctccctcaagaaccgagtcaccataacgagagacacgtccaagaaccaattttccctgaggttgacttctgtgaccgctgcggacacggccgtatattactgt gcgaggggcggtggctggaccccctactttgactcc unmatched 28 216 19 9 12 16 7 12 7 2 14 5 JY8QFUQ01CKN3U 12807 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F 860 88.53 193/218 nt 87.61 191/218 nt Homsap IGHJ6*02 F 193 79.03 49/62 nt Homsap IGHD6-6*01 F 1 8 8 17 8.8.17 [1.17.38.11] CARDSGSSDYTRYDAQDVW in-frame - Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 29 of V-REGION: 'G' (1 nucleotides at position 14 to 14 in the user submitted sequence), (cause frameshift);in CDR1-IMGT at codon 38 of V-REGION: 'G' (1 nucleotides at position 29 to 29 in the user submitted sequence), (cause frameshift); cctctggattcagGttttagttcttatgGgcatgaactgggtccgccaggctccatggggggggctggagtgggtctcattcattaacagtgttagtagttacaaatactatgtggacccagtgaggggccgattcaccatctccagagacaacgccaagaacgcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtttactactgtgcgagagatagcggctcgtccgactacactaggtacgacgcccaggacgtctggggccaagggaccgcggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgaaagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaac NA unmatched, IGG2 64 88 [303] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>t|a106>t|g107>c|c108>t|a112>g,S38>G(+ + -)|g139>t,G47>W(- - -)|a142>g,K48>G(- - -)|a143>g,K48>G(- - -)|c164>t,S55>F(- - -)|g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|t194>a,I65>K(- + -)|c201>t|c203>t,A68>V(+ - +)|a204>g,A68>V(+ - +)|t208>c,S70>P(+ - -)|a215>g,K72>R(+ + +)|t256>g,S86>A(- + -)|c265>t|g303>t|t306>c| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|c90>t|a106>t|g107>c|c108>t|a112>g,S38>G(+ + -)| g139>t,G47>W(- - -)|a142>g,K48>G(- - -)|a143>g,K48>G(- - -)|c164>t,S55>F(- - -)| g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|a175>g,S59>V(- - -)|g176>t,S59>V(- - -)|t194>a,I65>K(- + -)| c201>t|c203>t,A68>V(+ - +)|a204>g,A68>V(+ - +)|t208>c,S70>P(+ - -)|a215>g,K72>R(+ + +)|t256>g,S86>A(- + -)|c265>t|g303>t|t306>c| 320 226 201 25 8 17 6 1 7 3 0 0 1 1 1 1 3 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 4 3 1 0 3 0 0 0 1 0 1 1 0 0 51 51 47 4 0 4 2 0 1 0 0 0 0 0 0 0 1 0 30 24 19 5 0 5 1 1 0 1 0 0 0 1 0 0 1 0 117 114 105 9 4 5 2 0 3 2 0 0 0 0 0 0 1 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcagttttagttcttatggc atgaactgggtccgccaggctccatggggggggctggagtgggtctcattc attaacagtgttagtagttacaaa tactatgtggacccagtgaggggccgattcaccatctccagagacaacgccaagaacgcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtttactactgt gcgagagatagcggctcgtccgactacactaggtacgacgcccaggacgtc ARDSGSSDYTRYDAQDV IGHV3-21 IGHD6-6 IGHJ6 ggattcagttttagttcttatggc atgaactgggtccgccaggctccatggggggggctggagtgggtctcattc attaacagtgttagtagttacaaa tactatgtggacccagtgaggggccgattcaccatctccagagacaacgccaagaacgcactgtatttgcaaatgaacagcctgagagccgaggacacggctgtttactactgt gcgagagatagcggctcgtccgactacactaggtacgacgcccaggacgtc unmatched 25 213 17 8 8 13 9 12 4 4 9 8 JY8QFUQ01CLP4K 15879 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 954 93.55 203/217 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD3-3*01 F 3 8 8 11 8.8.11 [1.17.38.11] CARFGPLTRPDYW in-frame + ctctggattcacctttatcaactatggcatgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggtattagtggtagtggtgataccacataccacgcagactccgtgcagggccgattcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagtcgaggacacggccgtttattactgtgcgagattcggcccacttactcgacctgactactggggccagggaactttggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [281, 282] g104>t,S35>I(- - -)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)|c164>g,A55>G(- + -)|g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)|t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|g225>a|t246>c|g258>t|c279>t|c287>t,A96>V(+ - +)|a303>t| g104>t,S35>I(- - -)|g107>a,S36>N(- - -)|c113>g,A38>G(- + -)| c164>g,A55>G(- + -)| g188>a,G63>D(- - -)|g191>c,S64>T(+ - +)| t199>c,Y67>H(+ - -)|a214>c,K72>Q(+ - -)|g225>a|t246>c|g258>t|c279>t|c287>t,A96>V(+ - +)|a303>t| a317>g,K106>R(+ + +)|g319>t|a320>t| 316 (320) 221 (225) 207 (208) 14 (17) 5 (7) 9 (10) 0 (1) 3 2 2 1 0 1 (2) 0 1 2 2 (3) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 0 1 1 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 1 0 0 0 117 114 106 8 5 3 0 1 2 2 1 0 1 0 0 0 1 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttatcaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgataccaca taccacgcagactccgtgcagggccgattcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagtcgaggacacggccgtttattactgt gcgagattcggcccacttactcgacctgactac ARFGPLTRPDY IGHV3-23 IGHD3-3 IGHJ4 ggattcacctttatcaactatggc atgagctgggtccgccaggctccagggaaggggctggagtgggtctcaggt attagtggtagtggtgataccaca taccacgcagactccgtgcagggccgattcaccatctccagagacaactccaagaacactctgtatctgcaaatgaacagtctgagagtcgaggacacggccgtttattactgt gcgagattcggcccacttactcgacctgactac unmatched 14 213 7 7 5 10 2 4 5 0 4 5 JY8QFUQ01CNCW4 12906 productive (see comment) Homsap IGHV1-24*01 F 970 95.31 203/213 nt 94.84 202/213 nt Homsap IGHJ5*02 F 141 77.08 37/48 nt Homsap IGHD5-18*01 F 1 8 8 7 8.8.7 [X.17.38.11] CAIGAMATW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 57 of V-REGION: 'C' (1 nucleotides at position 80 to 80 in the user submitted sequence), (cause frameshift); cggaaaaccctcactgaagtatccatgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggaggatttgCatcctgaagatggtgaaataatctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgtgcaataggagctatggccacctggggccagggaaccctggtcaccgtcgactcagcatccccgaccagcccaaggtcttcccgctgagcctctgcaagcaccccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttcccc NA unmatched, IGA1 64 79 [267, 265, 266] g79>c,G27>R(- - -)|a81>g,G27>R(- - -)|t82>a,Y28>K(- - -)|c84>a,Y28>K(- - -)|t109>g,L37>V(+ - +)|t165>a|c194>t,T65>I(- - -)|g226>a,V76>I(+ - +)|a232>g,M78>V(+ - -)|g291>a|c317>t,T106>I(- - -)| g79>c,G27>R(- - -)|a81>g,G27>R(- - -)|t82>a,Y28>K(- - -)|c84>a,Y28>K(- - -)|t109>g,L37>V(+ - +)| t165>a| c194>t,T65>I(- - -)| g226>a,V76>I(+ - +)|a232>g,M78>V(+ - -)|g291>a| c317>t,T106>I(- - -)|a320>g| 319 (320) 220 (221) 209 11 (12) 2 (3) 9 2 (3) 2 2 0 0 1 0 2 1 0 0 1 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 0 5 1 0 0 0 0 1 0 1 1 0 0 1 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 117 114 111 3 1 2 1 2 0 0 0 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,14-15(FR1)|aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|aa,107-108(CDR1)|ta,110-111(CDR1)|aa,141-142(FR2)|aa,143-144(FR2)|aa,176-177(CDR2)|aa,191-192(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,249-250(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,14-15(FR1)|tt,74-75(FR1)|ta,82-83(CDR1)|tt,109-110(CDR1)|tt,149-150(FR2)|tt,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,249-250(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggta,12-15(FR1)|ggct,24-27(FR1)|ggtt,72-75(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,163-166(FR2-CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| cggaaaaccctcactgaagtatcc atgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggagga tttgatcctgaagatggtgaaata atctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcaataggagctatggccacc AIGAMAT IGHV1-24 IGHD5-18 IGHJ5 cggaaaaccctcactgaagtatcc atgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggagga tttgatcctgaagatggtgaaata atctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcaataggagctatggccacc unmatched 10 213 5 5 3 5 2 5 2 0 2 6 JY8QFUQ01CO019 10768 productive Homsap IGHV3-11*01 F 931 92.59 200/216 nt NA Homsap IGHJ4*02 F 186 87.5 42/48 nt Homsap IGHD3-10*01 F 3 8 8 14 8.8.14 [1.17.38.11] CTRDDRSVRGVTSDYW in-frame + tctggattcaccttcagtgaccacttcatgagttggatccgccaggctccagggaaggggctggagtgggtttcatacattagtggcagtggtagtataatatattacgcagactctgtgaggggccgattcaccatctccagggacaacgccaagaattccctctatctgcaaatggacagcctgagagacgaggacacggccgtgtatttttgtacgagagatgatcgctcggttcggggagtcacctcagactactggggccagggaaccctggtcaccgtctcctccgcctcctggcaccctcctccaagagcacctctggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggcaactcagg NA unmatched, IGG1 71 50 [265] t109>c,Y37>H(+ - -)|a113>t,Y38>F(- + -)|c120>t|a172>g,S58>G(+ + -)|t174>c,S58>G(+ + -)|c191>t,T64>I(- - -)|c192>a,T64>I(- - -)|c198>t|a215>g,K72>R(+ + +)|c255>t|a258>c|g261>c|a274>g,N92>D(+ + -)|c287>a,A96>D(- - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)|g313>a,A105>T(- - -)| t109>c,Y37>H(+ - -)|a113>t,Y38>F(- + -)| c120>t| a172>g,S58>G(+ + -)|t174>c,S58>G(+ + -)|c191>t,T64>I(- - -)|c192>a,T64>I(- - -)| c198>t|a215>g,K72>R(+ + +)|c255>t|a258>c|g261>c|a274>g,N92>D(+ + -)|c287>a,A96>D(- - -)|a308>t,Y103>F(- + -)|c309>t,Y103>F(- + -)| g313>a,A105>T(- - -)| 320 224 207 17 5 12 3 1 5 2 1 2 2 0 1 0 0 0 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 1 0 0 1 0 0 0 0 0 51 51 50 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 1 1 0 1 0 0 0 0 0 0 117 114 105 9 4 5 2 0 3 0 1 1 1 0 1 0 0 0 8 8 7 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcaccttcagtgaccacttc atgagttggatccgccaggctccagggaaggggctggagtgggtttcatac attagtggcagtggtagtataata tattacgcagactctgtgaggggccgattcaccatctccagggacaacgccaagaattccctctatctgcaaatggacagcctgagagacgaggacacggccgtgtatttttgt acgagagatgatcgctcggttcggggagtcacctcagactac TRDDRSVRGVTSDY IGHV3-11 IGHD3-10 IGHJ4 ggattcaccttcagtgaccacttc atgagttggatccgccaggctccagggaaggggctggagtgggtttcatac attagtggcagtggtagtataata tattacgcagactctgtgaggggccgattcaccatctccagggacaacgccaagaattccctctatctgcaaatggacagcctgagagacgaggacacggccgtgtatttttgt acgagagatgatcgctcggttcggggagtcacctcagactac unmatched 16 213 10 6 5 8 5 8 5 0 5 6 JY8QFUQ01CPVUP 19417 productive Homsap IGHV3-48*03 F 855 88.48 192/217 nt NA Homsap IGHJ6*02 F 211 82.26 51/62 nt Homsap IGHD3-3*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARDKGWSGYGMDVW in-frame + ctctggattcaccttcagttcttatgccatgaactgggtccgcctggttccaggcaaggggctggaatggctttcatttattggtaatactggtagtgtcatatactacgcagactctgtgaaggggcgattcaccatctccagagacaatgccaagaactcaatgtctctacaaatgagcagcctgagagccgaggacacggctctatattattgtgcgagagataaaggttggagcggatacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcatccccggaccagccccaaggtcttcccgctgagcctcgacagcacccccccaaaggatgggaacgtggtcatcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 84 [288, 291] a106>t|g107>c|a113>c,E38>A(- - -)|a114>c,E38>A(- - -)|a131>t,Q44>L(- - -)|c134>t,A45>V(+ - +)|g141>c|g153>a|g157>c,V53>L(+ - +)|a164>t,Y55>F(- + -)|c165>t,Y55>F(- + -)|a169>g,S57>G(+ + -)|g173>a,S58>N(- - -)|g176>c,S59>T(+ - +)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)|c222>g|c246>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|a275>g,N92>S(- - -)|g301>c,V101>L(+ - +)|t303>a,V101>L(+ - +)|c309>t| a106>t|g107>c|a113>c,E38>A(- - -)|a114>c,E38>A(- - -)| a131>t,Q44>L(- - -)|c134>t,A45>V(+ - +)|g141>c|g153>a|g157>c,V53>L(+ - +)|a164>t,Y55>F(- + -)|c165>t,Y55>F(- + -)| a169>g,S57>G(+ + -)|g173>a,S58>N(- - -)|g176>c,S59>T(+ - +)|a190>g,T64>V(- - -)|c191>t,T64>V(- - -)| c222>g|c246>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|a275>g,N92>S(- - -)|g301>c,V101>L(+ - +)|t303>a,V101>L(+ - +)|c309>t| 320 225 200 25 8 17 3 3 5 0 3 1 3 1 5 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 2 2 0 0 0 0 2 0 1 0 1 0 0 0 51 51 44 7 2 5 0 1 2 0 0 0 2 0 2 0 0 0 30 24 19 5 0 5 2 1 1 0 0 0 0 0 1 0 0 0 117 114 105 9 4 5 1 1 2 0 1 1 0 1 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,113-114(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|tt,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|ggta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagttcttatgcc atgaactgggtccgcctggttccaggcaaggggctggaatggctttcattt attggtaatactggtagtgtcata tactacgcagactctgtgaaggggcgattcaccatctccagagacaatgccaagaactcaatgtctctacaaatgagcagcctgagagccgaggacacggctctatattattgt gcgagagataaaggttggagcggatacggtatggacgtc ARDKGWSGYGMDV IGHV3-48 IGHD3-3 IGHJ6 ggattcaccttcagttcttatgcc atgaactgggtccgcctggttccaggcaaggggctggaatggctttcattt attggtaatactggtagtgtcata tactacgcagactctgtgaaggggcgattcaccatctccagagacaatgccaagaactcaatgtctctacaaatgagcagcctgagagccgaggacacggctctatattattgt gcgagagataaaggttggagcggatacggtatggacgtc unmatched 25 213 11 14 8 15 3 10 6 2 10 7 JY8QFUQ01CPYJ0 13333 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F 954 93.55 203/217 nt NA Homsap IGHJ2*01 F 220 90.57 48/53 nt Homsap IGHD1-26*01 F 3 8 8 12 8.8.12 [1.17.38.11] CARPFSGRWCFDLW in-frame + ctctggattcaccttcagttactcctggatgcactgggtccgccaagttccaggaaaggggccggtgtgggtctcacaaattaaaagtgatgggagtaccccaagttacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagtcgaggacacggctgtttattactgtgcaagaccgtttagtgggaggtggtgcttcgatctctggggccgtggcaccctggtcactgtctcctcagcatccccgaccagccccaaggtcttccccgctgagccctcgacagcaccccccaaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 64 84 [284, 287, 286] a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a110>c,Y37>S(+ - -)|c134>t,A45>V(+ - +)|g141>a|t149>c,L50>P(- - -)|g164>a,R55>Q(+ - -)|t165>a,R55>Q(+ - -)|t171>a,N57>K(+ - -)|g191>c,S64>T(+ - +)|a193>c,T65>P(+ + -)|c198>t|c287>t,A96>V(+ - +)|g303>t| a106>t,S36>Y(+ - -)|g107>a,S36>Y(+ - -)|a110>c,Y37>S(+ - -)| c134>t,A45>V(+ - +)|g141>a|t149>c,L50>P(- - -)|g164>a,R55>Q(+ - -)|t165>a,R55>Q(+ - -)| t171>a,N57>K(+ - -)|g191>c,S64>T(+ - +)|a193>c,T65>P(+ + -)| c198>t|c287>t,A96>V(+ - +)|g303>t| g319>c|a320>c| 318 (320) 223 (225) 209 14 (16) 3 (5) 11 0 3 3 1 2 (3) 0 1 2 1 (2) 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 1 0 1 0 0 0 0 0 51 51 46 5 1 4 0 2 1 1 0 0 0 1 0 0 0 0 30 24 21 3 0 3 0 0 0 0 1 0 0 1 1 0 0 0 117 114 111 3 2 1 0 0 2 0 0 0 0 0 0 0 1 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagttactcctgg atgcactgggtccgccaagttccaggaaaggggccggtgtgggtctcacaa attaaaagtgatgggagtacccca agttacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagtcgaggacacggctgtttattactgt gcaagaccgtttagtgggaggtggtgcttcgatctc ARPFSGRWCFDL IGHV3-74 IGHD1-26 IGHJ2 ggattcaccttcagttactcctgg atgcactgggtccgccaagttccaggaaaggggccggtgtgggtctcacaa attaaaagtgatgggagtacccca agttacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgagagtcgaggacacggctgtttattactgt gcaagaccgtttagtgggaggtggtgcttcgatctc unmatched 14 213 7 7 6 8 1 6 3 0 5 6 JY8QFUQ01CQH14 17914 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 954 93.55 203/217 nt NA Homsap IGHJ6*02 F 220 83.87 52/62 nt Homsap IGHD5-12*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARGPRSRGYGMDVW in-frame + ctctggattcaccttcaacaactatgccatgagttgggtccgccaggctccagggaaggggctggaatgggtctcaactattactagtggtggtggtagtacattgtacgcagactccgtgaagggccggttcaccatctccagagacaatttcaaggacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgcgagagggcctcgatcgcgcggctacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctctaagagcacctctgggggccaccagcggccctgggctgcctggtcaaggactcttccccggaaccggtgacggtgtcgtggaactcagg NA unmatched, IGG1 64 75 [288] t90>c|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)|c120>t|g153>a|g163>a,A55>T(- - -)|g170>c,S57>T(+ - +)|g172>a,G58>S(+ + -)|a175>g,S59>G(+ + -)|c192>t|a197>t,Y66>L(- - -)|c198>g,Y66>L(- - -)|c248>t,S83>F(- - -)|a253>g,N85>D(+ + -)|a317>g,K106>R(+ + +)| t90>c|g104>a,S35>N(- - -)|g107>a,S36>N(- - -)| c120>t|g153>a|g163>a,A55>T(- - -)| g170>c,S57>T(+ - +)|g172>a,G58>S(+ + -)|a175>g,S59>G(+ + -)|c192>t| a197>t,Y66>L(- - -)|c198>g,Y66>L(- - -)|c248>t,S83>F(- - -)|a253>g,N85>D(+ + -)| a317>g,K106>R(+ + +)|a320>g| 319 (320) 224 (225) 209 15 (16) 4 (5) 11 3 (4) 5 3 1 0 0 1 0 1 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 1 2 0 2 0 1 0 0 0 0 0 0 0 0 51 51 48 3 2 1 0 2 1 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 1 1 1 0 0 0 0 0 1 0 0 0 117 114 110 4 0 4 1 0 1 0 0 0 1 0 0 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 1 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcaacaactatgcc atgagttgggtccgccaggctccagggaaggggctggaatgggtctcaact attactagtggtggtggtagtaca ttgtacgcagactccgtgaagggccggttcaccatctccagagacaatttcaaggacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagagggcctcgatcgcgcggctacggtatggacgtc ARGPRSRGYGMDV IGHV3-23 IGHD5-12 IGHJ6 ggattcaccttcaacaactatgcc atgagttgggtccgccaggctccagggaaggggctggaatgggtctcaact attactagtggtggtggtagtaca ttgtacgcagactccgtgaagggccggttcaccatctccagagacaatttcaaggacacgctgtatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgt gcgagagggcctcgatcgcgcggctacggtatggacgtc unmatched 14 213 11 3 8 10 3 4 2 2 5 5 JY8QFUQ01CST8T 5448 productive Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 918 91.71 199/217 nt NA Homsap IGHJ4*02 F 132 75 36/48 nt Homsap IGHD3-3*01 F 2 8 8 10 8.8.10 [1.17.38.11] CASDLGGQKSYW in-frame + ctcaggattcaccttcagtagatactggatgcactgggtccgccaagctccagggaaggggccggtgtgggtctcacgtactaatgaagatggcacccacataaattacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtatttgcaaatgaacagtctgagagccgaggacacggctgtctattactgtgcaagcgatttgggtggtcagaaaagttactggggccagggagtcctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcacccccccgaagatgggaacgtagtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 64 84 [278, 280] t78>a|c108>a,S36>R(- - -)|t149>c,L50>P(- - -)|t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>c|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|c194>t,T65>I(- - -)|g197>a,S66>N(- - -)|c198>t,S66>N(- - -)|c257>a,T86>K(- - -)|c265>t|g303>c|a318>c,R106>S(- - -)| t78>a| c108>a,S36>R(- - -)| t149>c,L50>P(- - -)| t167>c,I56>T(- - -)|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|g186>c|g188>c,S63>T(+ - +)|t189>c,S63>T(+ - +)|a190>c,S64>H(+ - -)|g191>a,S64>H(+ - -)|c194>t,T65>I(- - -)| g197>a,S66>N(- - -)|c198>t,S66>N(- - -)|c257>a,T86>K(- - -)|c265>t|g303>c| a318>c,R106>S(- - -)| 320 225 206 19 4 15 1 3 3 3 2 2 0 2 3 0 0 0 78 4 3 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 36 24 23 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 51 51 50 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 30 24 14 10 1 9 1 2 1 2 1 0 0 1 2 0 0 0 117 114 109 5 2 3 0 1 2 0 0 1 0 0 1 0 0 0 8 8 7 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctca ggattcaccttcagtagatactgg atgcactgggtccgccaagctccagggaaggggccggtgtgggtctcacgt actaatgaagatggcacccacata aattacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtatttgcaaatgaacagtctgagagccgaggacacggctgtctattactgt gcaagcgatttgggtggtcagaaaagttac ASDLGGQKSY IGHV3-74 IGHD3-3 IGHJ4 ggattcaccttcagtagatactgg atgcactgggtccgccaagctccagggaaggggccggtgtgggtctcacgt actaatgaagatggcacccacata aattacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacaagctgtatttgcaaatgaacagtctgagagccgaggacacggctgtctattactgt gcaagcgatttgggtggtcagaaaagttac unmatched 17 213 10 7 6 11 4 6 2 1 4 10 JY8QFUQ01CU1XI 2464 productive (see comment) Homsap IGHV3-53*01 F, or Homsap IGHV3-53*02 F 889 91.08 194/213 nt 90.61 193/213 nt Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD4-23*01 ORF 3 8 6 12 8.6.12 [1.17.38.11] CASSVFSPGGFDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 56 of V-REGION: 1 nucleotides (from position 80 in the user submitted sequence), (cause frameshift) ttcggggttcaccgtcagtagcaagtacatgacctgggtccgccaggctccggggaagggactggagtctgtctctgtttttatagcggtgatcaaacatactacgcagactccgtgaggggccgattcaccatctccatagacaattccaagaacacactgtatcttcaaatgaacggcctgcgagccgaggacacggccgtgtattattgtgcgagctccgtgtttagtcccggaggctttgactactggggccagggaaccctggtcaccgtctcctcagcatccccggaccagccccaaggtcttcccgctgagcctcgacagcacccccctaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccag NA unmatched, IGA2 57 84 [281, 282] c75>t|t78>g|c111>g,N37>K(+ - -)|g119>c,S40>T(+ - +)|a138>g|g147>a|g155>c,W52>S(- - -)|g156>t,W52>S(- - -)|a162>t|g188>a,G63>D(- - -)|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>a,S64>Q(- - -)|a215>g,K72>R(+ + +)|g239>t,R80>I(- + -)|g258>a|a277>g,S93>G(+ + -)|a283>c|c309>t| c75>t|t78>g| c111>g,N37>K(+ - -)| g119>c,S40>T(+ - +)|a138>g|g147>a|g155>c,W52>S(- - -)|g156>t,W52>S(- - -)|a162>t| g188>a,G63>D(- - -)|a190>c,S64>Q(- - -)|g191>a,S64>Q(- - -)|c192>a,S64>Q(- - -)| a215>g,K72>R(+ + +)|g239>t,R80>I(- + -)|g258>a|a277>g,S93>G(+ + -)|a283>c|c309>t| a318>c,R106>S(- - -)|g319>t|a320>c| 317 (320) 218 (221) 199 19 (22) 8 (10) 11 (12) 3 4 2 0 2 (4) 1 1 0 2 1 2 (3) 1 78 4 2 2 2 0 0 0 1 0 0 0 0 0 0 0 0 1 36 24 23 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 51 51 45 6 3 3 1 1 0 0 0 0 1 0 2 0 1 0 30 20 16 4 0 4 0 2 0 0 1 1 0 0 0 0 0 0 117 114 108 6 3 3 2 1 1 0 1 0 0 0 0 0 1 0 5 (8) 5 5 0 (3) 0 (2) 0 (1) 0 0 0 0 0 (2) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggtt,80-83(CDR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ttcg gggttcaccgtcagtagcaagtac atgacctgggtccgccaggctccggggaagggactggagtctgtctctgtt tttatagcggtgatcaaaca tactacgcagactccgtgaggggccgattcaccatctccatagacaattccaagaacacactgtatcttcaaatgaacggcctgcgagccgaggacacggccgtgtattattgt gcgagctccgtgtttagtcccggaggctttgactac ASSVFSPGGFDY IGHV3-53 IGHD4-23 IGHJ4 gggttcaccgtcagtagcaagtac atgacctgggtccgccaggctccggggaagggactggagtctgtctctgtt tttatagcggtgatcaaaca tactacgcagactccgtgaggggccgattcaccatctccatagacaattccaagaacacactgtatcttcaaatgaacggcctgcgagccgaggacacggccgtgtattattgt gcgagctccgtgtttagtcccggaggctttgactac unmatched 17 209 8 9 5 11 3 6 6 0 6 5 JY8QFUQ01CU5CB 21097 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 905 90.83 198/218 nt 89.91 196/218 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD5-12*01 F 3 8 8 10 8.8.10 [1.17.38.11] CARDLYGADDLW in-frame + Potentially, because of detected insertion/deletion in CDR1-IMGT at codon 30 of V-REGION: 'T' (1 nucleotides at position 16 to 16 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 89 of V-REGION: 'T' (1 nucleotides at position 172 to 172 in the user submitted sequence), (cause frameshift); cctctggattcacctTttagtagatattggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtattaatgaagatgggaggaccacaacctacgcggactccgtgaatggccgattcctcatctccagagacaacgccaagaatacgttgtatTctgcagatgagcagtctgagagtcgaggacacggccatgtattattgtgcaagagatctatatggtgccgatgacctctggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgncagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccca NA unmatched, IGA2 29 79 [281, 282, 273] c90>t|c108>a,S36>R(- - -)|c111>t|a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t189>g,S63>R(- - -)|g191>c,S64>T(+ - +)|g197>c,S66>T(+ - +)|g216>t,K72>N(+ - -)|a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|c255>t|c259>t|a270>g|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|t300>c|g301>a,V101>M(+ - -)|c309>t| c90>t|c108>a,S36>R(- - -)|c111>t| a172>g,S58>E(- - -)|g173>a,S58>E(- - -)|t174>a,S58>E(- - -)|t189>g,S63>R(- - -)|g191>c,S64>T(+ - +)| g197>c,S66>T(+ - +)|g216>t,K72>N(+ - -)|a229>c,T77>L(- - -)|c230>t,T77>L(- - -)|c255>t|c259>t|a270>g|a275>g,N92>S(- - -)|c287>t,A96>V(+ - +)|t300>c|g301>a,V101>M(+ - -)|c309>t| 320 226 206 20 7 13 3 2 7 1 1 1 0 1 2 0 1 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 2 0 0 1 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 19 5 0 5 1 1 0 0 0 0 0 1 1 0 0 1 117 114 102 12 5 7 2 1 5 1 1 0 0 0 1 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcacctttagtagatattgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attaatgaagatgggaggaccaca acctacgcggactccgtgaatggccgattcctcatctccagagacaacgccaagaatacgttgtatctgcagatgagcagtctgagagtcgaggacacggccatgtattattgt gcaagagatctatatggtgccgatgacctc ARDLYGADDL IGHV3-74 IGHD5-12 IGHJ4 ggattcacctttagtagatattgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt attaatgaagatgggaggaccaca acctacgcggactccgtgaatggccgattcctcatctccagagacaacgccaagaatacgttgtatctgcagatgagcagtctgagagtcgaggacacggccatgtattattgt gcaagagatctatatggtgccgatgacctc unmatched 20 213 13 7 9 13 4 7 5 2 7 6 JY8QFUQ01CUGFD 16670 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23*05 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 806 85.78 187/218 nt NA Homsap IGHJ4*02 F 159 81.25 39/48 nt Homsap IGHD1-20*01 F 1 8 8 14 8.8.14 [1.17.38.11] CARGLTTMTGNGLEHW in-frame + tctctggattcacccttcgcagatatggcatggcgtgggtccgccaggctccggggaaggggctggagtgggtctcatcttctaacagtagtgatgaatccacatactatgcagactccgtgaagggccgcttcaccatttccagagaccattccaagaacacggtgtttttgcaaatgtacagcctgagagccgaagacacggccctctattactgtgcgagagggctaacaacaatgacagggaatggtcttgagcactggggccagggagccctggtcaccgtctcctcaccatccccggaccagccccaagggtcttcccgctgagcctcgacagcacccccccaaagatgggaacgtggtngtcgcatgcctcgtcccagggcttcttcccccag NA unmatched, IGA2 57 68 [293, 287] c74>t|t88>c,F30>L(+ - -)|a103>c,S35>R(- - -)|c108>a,S36>R(- - -)|c113>g,A38>G(- + -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c120>g,S40>A(- + -)|a138>g|g163>t,A55>S(- + -)|a166>t,I56>S(- - -)|t167>c,I56>S(- - -)|g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|g172>a,G58>S(+ + -)|g185>a,G62>D(- - -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|a190>t|g191>c|c201>t|g225>c|c234>t|a244>c,N82>H(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c265>t|a274>t,N92>Y(- - -)|g291>a|g301>c,V101>L(+ - +)|a303>c,V101>L(+ - +)|a317>g,K106>R(+ + +)| c74>t| t88>c,F30>L(+ - -)|a103>c,S35>R(- - -)|c108>a,S36>R(- - -)|c113>g,A38>G(- + -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c120>g,S40>A(- + -)|a138>g|g163>t,A55>S(- + -)| a166>t,I56>S(- - -)|t167>c,I56>S(- - -)|g170>a,S57>N(- - -)|t171>c,S57>N(- - -)|g172>a,G58>S(+ + -)|g185>a,G62>D(- - -)|g188>a,G63>E(- - -)|t189>a,G63>E(- - -)|a190>t|g191>c| c201>t|g225>c|c234>t|a244>c,N82>H(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c265>t|a274>t,N92>Y(- - -)|g291>a|g301>c,V101>L(+ - +)|a303>c,V101>L(+ - +)| a317>g,K106>R(+ + +)|a320>g| 319 (320) 225 (226) 193 32 (33) 9 (10) 23 3 (4) 5 4 3 3 1 4 1 4 3 1 0 78 5 4 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 0 0 0 1 1 1 0 0 0 1 0 0 51 51 46 5 1 4 2 0 0 0 0 0 0 0 1 1 1 0 30 24 14 10 2 8 0 4 0 2 0 0 2 1 1 0 0 0 117 114 103 11 5 6 0 1 3 0 2 0 2 0 2 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 1 (2) 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tctct ggattcacccttcgcagatatggc atggcgtgggtccgccaggctccggggaaggggctggagtgggtctcatct tctaacagtagtgatgaatccaca tactatgcagactccgtgaagggccgcttcaccatttccagagaccattccaagaacacggtgtttttgcaaatgtacagcctgagagccgaagacacggccctctattactgt gcgagagggctaacaacaatgacagggaatggtcttgagcac ARGLTTMTGNGLEH IGHV3-23 IGHD1-20 IGHJ4 ggattcacccttcgcagatatggc atggcgtgggtccgccaggctccggggaaggggctggagtgggtctcatct tctaacagtagtgatgaatccaca tactatgcagactccgtgaagggccgcttcaccatttccagagaccattccaagaacacggtgtttttgcaaatgtacagcctgagagccgaagacacggccctctattactgt gcgagagggctaacaacaatgacagggaatggtcttgagcac unmatched 30 213 13 17 8 17 5 13 6 2 10 12 JY8QFUQ01CURPS 4763 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*02 F or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 787 85.19 184/216 nt NA Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F (see comment) 159 81.25 39/48 nt Homsap IGHD3-16*02 F 2 8 8 17 8.8.17 [1.17.38.11] CVKDRDFLWGSYRLQLESW in-frame + Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) tctggattcacctttagtagctatggcatgagttgggtccgccagtctccaaataagggactggagtgggtcgcaggcattagtgcaaatggtggcagtataaattatctggacgccgtgaagggccggtttatcatctctagagacaattccaagaacacgttgtatctgcaaatggacagcctgacagtcgaggacacggccgtttattactgtgtgaaagatcgtgattttctttgggggagctatcgtcttcaacttgaatcttggggccaggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacacgcacccccaagcatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 43 74 [298, 299] c105>t|c113>g,A38>G(- + -)|c120>t|g133>t,A45>S(- + -)|g139>a,G47>N(- - -)|g140>a,G47>N(- - -)|g141>t,G47>N(- - -)|g147>a|t160>g,S54>A(- + -)|c164>g,A55>G(- + -)|t165>c,A55>G(- + -)|g173>c,G58>A(- + -)|t174>a,G58>A(- + -)|g176>a,S59>N(- - -)|t189>c|c192>t|c194>t,T65>I(- - -)|t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|c201>t|g202>c,A68>L(+ - +)|c203>t,A68>L(+ - +)|a204>g,A68>L(+ - +)|t208>g,S70>A(- + -)|c228>t|c230>t,T77>I(- - -)|c237>t|c259>t|a274>g,N92>D(+ + -)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|a303>t|c314>t,A105>V(+ - +)| c105>t|c113>g,A38>G(- + -)| c120>t|g133>t,A45>S(- + -)|g139>a,G47>N(- - -)|g140>a,G47>N(- - -)|g141>t,G47>N(- - -)|g147>a|t160>g,S54>A(- + -)|c164>g,A55>G(- + -)|t165>c,A55>G(- + -)| g173>c,G58>A(- + -)|t174>a,G58>A(- + -)|g176>a,S59>N(- - -)|t189>c|c192>t|c194>t,T65>I(- - -)| t196>a,Y66>N(- - -)|c198>t,Y66>N(- - -)|c201>t|g202>c,A68>L(+ - +)|c203>t,A68>L(+ - +)|a204>g,A68>L(+ - +)|t208>g,S70>A(- + -)|c228>t|c230>t,T77>I(- - -)|c237>t|c259>t|a274>g,N92>D(+ + -)|g284>c,R95>T(- - -)|c287>t,A96>V(+ - +)|a303>t| c314>t,A105>V(+ - +)| 320 224 191 33 10 23 2 4 13 2 0 0 1 2 3 2 2 2 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 1 1 0 0 1 0 0 0 0 0 0 1 0 0 51 51 42 9 2 7 0 3 1 1 0 0 0 0 0 1 2 1 30 24 18 6 2 4 0 1 2 1 0 0 0 1 1 0 0 0 117 114 99 15 5 10 2 0 8 0 0 0 1 1 2 0 0 1 8 8 7 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcacctttagtagctatggc atgagttgggtccgccagtctccaaataagggactggagtgggtcgcaggc attagtgcaaatggtggcagtata aattatctggacgccgtgaagggccggtttatcatctctagagacaattccaagaacacgttgtatctgcaaatggacagcctgacagtcgaggacacggccgtttattactgt gtgaaagatcgtgattttctttgggggagctatcgtcttcaacttgaatct VKDRDFLWGSYRLQLES IGHV3-23 IGHD3-16 IGHJ4 ggattcacctttagtagctatggc atgagttgggtccgccagtctccaaataagggactggagtgggtcgcaggc attagtgcaaatggtggcagtata aattatctggacgccgtgaagggccggtttatcatctctagagacaattccaagaacacgttgtatctgcaaatggacagcctgacagtcgaggacacggccgtttattactgt gtgaaagatcgtgattttctttgggggagctatcgtcttcaacttgaatct unmatched 32 213 20 12 16 23 4 9 7 3 17 5 JY8QFUQ01CY2WW 7665 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30*19 F or Homsap IGHV3-30-5*01 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD1-26*01 F 3 8 8 13 8.8.13 [1.17.38.11] CAKSPSQVGYYLDCW in-frame + ctctggattcaccttcagtacctttggcatgcactgggtccgccaggctcccggcaaggggctggagtgggtggcaatcatatcaaatgatggaagtaagaaatactacgcagactccgtgaagggccgattcaccatttccagagaaaattccgagaacacgctgtatctgcaaatgagcagcctgagagctgaggacacggctgtgtattactgtgcgaaatcaccctcccaagtgggctactacttagactgttggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaacgatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 84 [287, 289] g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)|a138>c|g163>a,V55>I(+ - +)|t165>c,V55>I(+ - +)|t172>a,Y58>N(- - -)|t192>g,N64>K(+ - -)|t201>c|c234>t|c243>a,D81>E(+ - +)|a250>g,K84>E(+ - -)|a275>g,N92>S(- - -)| g107>c,S36>T(+ - +)|a110>t,Y37>F(- + -)| a138>c|g163>a,V55>I(+ - +)|t165>c,V55>I(+ - +)| t172>a,Y58>N(- - -)|t192>g,N64>K(+ - -)| t201>c|c234>t|c243>a,D81>E(+ - +)|a250>g,K84>E(+ - -)|a275>g,N92>S(- - -)| g317>a,R106>K(+ + +)|g319>t|a320>c| 316 (320) 221 (225) 209 (210) 12 (15) 3 (5) 9 (10) 2 1 (2) 1 2 1 (2) 1 1 1 1 0 0 (1) 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 0 0 0 0 0 0 1 0 1 0 0 0 51 51 48 3 1 2 0 1 0 1 1 0 0 0 0 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 0 1 0 0 0 1 117 114 109 5 2 3 2 0 1 1 0 1 0 0 0 0 0 0 4 (8) 4 4 (5) 0 (3) 0 (2) 0 (1) 0 0 (1) 0 0 0 (1) 0 0 0 0 0 0 (1) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtacctttggc atgcactgggtccgccaggctcccggcaaggggctggagtgggtggcaatc atatcaaatgatggaagtaagaaa tactacgcagactccgtgaagggccgattcaccatttccagagaaaattccgagaacacgctgtatctgcaaatgagcagcctgagagctgaggacacggctgtgtattactgt gcgaaatcaccctcccaagtgggctactacttagactgt AKSPSQVGYYLDC IGHV3-30 IGHD1-26 IGHJ4 ggattcaccttcagtacctttggc atgcactgggtccgccaggctcccggcaaggggctggagtgggtggcaatc atatcaaatgatggaagtaagaaa tactacgcagactccgtgaagggccgattcaccatttccagagaaaattccgagaacacgctgtatctgcaaatgagcagcctgagagctgaggacacggctgtgtattactgt gcgaaatcaccctcccaagtgggctactacttagactgt unmatched 12 213 6 6 2 4 4 8 3 0 5 4 JY8QFUQ01CY6MC 7313 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 936 92.63 201/217 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD4-17*01 F 2 8 8 13 8.8.13 [1.17.38.11] CARPLRATYGDYVNW in-frame + ctctggattcaccttcgataactatggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagttatatcaaaggatggaagtattgaatactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacaatttatctgcaaatgaacattgtgagggttgaggacacggctatgtattactgtgcgagaccccttcgggcaacttacggtgactacgtgaactggggccagggaaccctggtcaccgtctcctcagcatcccctgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccag NA unmatched, IGA2 57 79 [288, 289] a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)|t172>a,Y58>K(- - -)|t174>g,Y58>K(- - -)|a191>t,N64>I(- - -)|a193>g,K65>E(+ - -)|g258>a|c259>a,L87>I(+ + +)|g261>t,L87>I(+ + +)|g278>t,S93>I(- - -)|c279>t,S93>I(- - -)|c280>g,L94>V(+ - +)|a285>g|c287>t,A96>V(+ - +)|g301>a,V101>M(+ - -)| a103>g,S35>D(- - -)|g104>a,S35>D(- - -)|g107>a,S36>N(- - -)| t172>a,Y58>K(- - -)|t174>g,Y58>K(- - -)|a191>t,N64>I(- - -)|a193>g,K65>E(+ - -)| g258>a|c259>a,L87>I(+ + +)|g261>t,L87>I(+ + +)|g278>t,S93>I(- - -)|c279>t,S93>I(- - -)|c280>g,L94>V(+ - +)|a285>g|c287>t,A96>V(+ - +)|g301>a,V101>M(+ - -)| g319>c|a320>c| 318 (320) 223 (225) 207 16 (18) 2 (4) 14 3 4 2 0 0 (1) 1 1 1 0 (1) 1 2 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 1 2 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 20 4 0 4 1 0 0 0 0 0 1 1 0 0 0 1 117 114 105 9 2 7 1 2 2 0 0 1 0 0 0 1 2 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcgataactatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcaaaggatggaagtattgaa tactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacaatttatctgcaaatgaacattgtgagggttgaggacacggctatgtattactgt gcgagaccccttcgggcaacttacggtgactacgtgaac ARPLRATYGDYVN IGHV3-30 IGHD4-17 IGHJ4 ggattcaccttcgataactatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcagtt atatcaaaggatggaagtattgaa tactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacaatttatctgcaaatgaacattgtgagggttgaggacacggctatgtattactgt gcgagaccccttcgggcaacttacggtgactacgtgaac unmatched 16 213 9 7 6 10 3 6 2 0 7 7 JY8QFUQ01CYWC2 11517 productive Homsap IGHV3-48*01 F, or Homsap IGHV3-48*02 F 972 94.47 205/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD1-7*01 F 3 8 8 10 8.8.10 [1.17.38.11] CARDRNYAFDYW in-frame + ctctggattcaccttcagcagctttagtatgaactgggtccgccaggctccagggaagggactggagtggctttcatacattagtaatactggtagtaacaaatactacgcagactctgtgaagggccgattcaccatctccagagacgatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgtgcgagagatcggaactacgctttcgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [278, 279] t105>c|a110>t,Y37>F(- + -)|c114>t|g147>a|g157>c,V53>L(+ - +)|g173>a,S58>N(- - -)|g176>c,S59>T(+ - +)|a184>g,S62>G(+ + -)|c191>a,T64>N(- + -)|t194>a,I65>K(- + -)|a244>g,N82>D(+ + -)|c287>t,A96>V(+ - +)| t105>c|a110>t,Y37>F(- + -)|c114>t| g147>a|g157>c,V53>L(+ - +)| g173>a,S58>N(- - -)|g176>c,S59>T(+ - +)|a184>g,S62>G(+ + -)|c191>a,T64>N(- + -)|t194>a,I65>K(- + -)| a244>g,N82>D(+ + -)|c287>t,A96>V(+ - +)| 320 225 213 12 3 9 2 2 2 1 0 1 1 1 2 0 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 2 1 0 0 1 1 0 0 1 0 0 0 0 0 51 51 49 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 30 24 19 5 0 5 1 1 0 0 0 1 0 1 1 0 0 0 117 114 112 2 0 2 1 0 1 0 0 0 0 0 0 0 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagcagctttagt atgaactgggtccgccaggctccagggaagggactggagtggctttcatac attagtaatactggtagtaacaaa tactacgcagactctgtgaagggccgattcaccatctccagagacgatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgt gcgagagatcggaactacgctttcgactac ARDRNYAFDY IGHV3-48 IGHD1-7 IGHJ4 ggattcaccttcagcagctttagt atgaactgggtccgccaggctccagggaagggactggagtggctttcatac attagtaatactggtagtaacaaa tactacgcagactctgtgaagggccgattcaccatctccagagacgatgccaagaactcactgtatctgcaaatgaacagcctgagagtcgaggacacggctgtgtattactgt gcgagagatcggaactacgctttcgactac unmatched 12 213 7 5 4 7 3 5 1 2 3 6 JY8QFUQ01DA9FW 14098 productive Homsap IGHV5-51*01 F, or Homsap IGHV5-51*03 F 959 93.58 204/218 nt NA Homsap IGHJ4*02 F (see comment) 177 85.42 41/48 nt Homsap IGHD5-12*01 F 1 8 8 9 8.8.9 [1.17.38.11] CARLPNTIDSW in-frame + Other possibilities: Homsap_IGHJ5*01 (highest number of consecutive identical nucleotides) cttctggatatagttttgccacctactggatcggctgggtgcgccagaggcccgggaagggcctggagtggatgggggtcatctatcctggtgactctgataccagatacagcccgtccttccaaggccaggtcaccatttcagccgacaagtccctcagtatcgcctacctgcagtggagcagcctgcaggcctcggacaccgccatatattactgtgcgagactgcctaacactattgactcctggggccaggggaccctggtcaacgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [276, 277] g74>c|c84>t|c87>t|a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)|t134>g,M45>R(- + -)|a144>g|a163>g,I55>V(+ - +)|c234>t|a250>c,I84>L(+ + +)|c255>t|c257>t,T86>I(- - -)|a283>c,K95>Q(+ - -)|g303>a,M101>I(+ + -)| g74>c| c84>t|c87>t|a103>g,T35>A(- - -)|g107>c,S36>T(+ - +)| t134>g,M45>R(- + -)|a144>g|a163>g,I55>V(+ - +)| c234>t|a250>c,I84>L(+ + +)|c255>t|c257>t,T86>I(- - -)|a283>c,K95>Q(+ - -)|g303>a,M101>I(+ + -)| a320>t| 319 (320) 225 (226) 211 14 (15) 6 (7) 8 3 1 5 0 2 0 0 (1) 0 2 0 0 1 78 5 4 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 36 24 20 4 2 2 1 0 2 0 0 0 0 0 1 0 0 0 51 51 48 3 1 2 2 0 0 0 0 0 0 0 0 0 0 1 30 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 2 4 0 1 3 0 2 0 0 0 0 0 0 0 7 (8) 7 7 0 (1) 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,37-38(FR1)|aa,39-40(FR1)|aa,58-59(FR1)|ta,69-70(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|aa,215-216(FR3)|aa,244-245(FR3)|ta,262-263(FR3)|aa,283-284(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| ta,69-70(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,169-170(CDR2)|ta,192-193(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agca,24-27(FR1)|ggtt,73-76(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|ggct,118-121(FR2)|agca,253-256(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,85-88(CDR1)|agct,106-109(CDR1)|tgcc,134-137(FR2)|tacc,192-195(CDR2)|agcc,202-205(FR3)|agcc,237-240(FR3)|tacc,262-265(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| cttct ggatatagttttgccacctactgg atcggctgggtgcgccagaggcccgggaagggcctggagtggatgggggtc atctatcctggtgactctgatacc agatacagcccgtccttccaaggccaggtcaccatttcagccgacaagtccctcagtatcgcctacctgcagtggagcagcctgcaggcctcggacaccgccatatattactgt gcgagactgcctaacactattgactcc ARLPNTIDS IGHV5-51 IGHD5-12 IGHJ4 ggatatagttttgccacctactgg atcggctgggtgcgccagaggcccgggaagggcctggagtggatgggggtc atctatcctggtgactctgatacc agatacagcccgtccttccaaggccaggtcaccatttcagccgacaagtccctcagtatcgcctacctgcagtggagcagcctgcaggcctcggacaccgccatatattactgt gcgagactgcctaacactattgactcc unmatched 13 213 9 4 6 7 3 6 3 2 6 2 JY8QFUQ01DA9S4 15943 productive Homsap IGHV4-39*01 F, or Homsap IGHV4-39*05 F 1100 100 221/221 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD5-18*01 F 3 10 7 12 10.7.12 [1.17.38.11] CARSRTFYGSANYW in-frame + tctctggtggctccatcagcagtagtagttactactggggctggatccgccagcccccagggaaggggctggagtggattgggagtatctattatagtgggagcacctactacaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggctgtgtattactgtgcgagaagtagaaccttctatggttcggccaactactggggccagggaaccctggtcaccgtctcctcagcctccacaagggcccatcggtctcccctggcaccctctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactc NA unmatched, IGG1 71 56 [285] c319>a|a320>g| 318 (320) 227 (229) 227 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 (1) 0 0 0 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccatcagcagtagtagttactac tggggctggatccgccagcccccagggaaggggctggagtggattgggagt atctattatagtgggagcacc tactacaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggctgtgtattactgt gcgagaagtagaaccttctatggttcggccaactac ARSRTFYGSANY IGHV4-39 IGHD5-18 IGHJ4 ggtggctccatcagcagtagtagttactac tggggctggatccgccagcccccagggaaggggctggagtggattgggagt atctattatagtgggagcacc tactacaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgccgcagacacggctgtgtattactgt gcgagaagtagaaccttctatggttcggccaactac unmatched 0 216 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01DC4QI 1765 productive Homsap IGHV3-48*01 F 891 90.32 196/217 nt NA Homsap IGHJ4*02 F, or Homsap IGHJ4*03 F (see comment) 150 79.17 38/48 nt Homsap IGHD2-21*01 F 1 8 8 12 8.8.12 [1.17.38.11] CARESTWEFTPDSW in-frame + Other possibilities: Homsap_IGHJ4*03 (highest number of consecutive identical nucleotides) ctctggattcagtttcagtgactatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagtagttctagtagtaccctatattatgcagactctgtgaagggccgattcaccgtctccagagacaatgacaagagttctctgtatctgcaaatgaccggcctgagagccgaagacacggcgacttattactgtgcgagagagtcgacgtgggaatttactcctgactcctggggcccggggaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcacccaggcccagcatgggaacgtggtcatcgcctgcctggtccagggcttcttcccccaag NA unmatched, IGA1 57 84 [284, 287] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|a175>t|g176>c|a193>c,I65>L(+ + +)|c198>t|c201>t|a232>g,I78>V(+ - +)|c248>a,A83>D(- - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a258>t|a275>c,N92>T(- + -)|a277>g,S93>G(+ + -)|g291>a|t300>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| a175>t|g176>c|a193>c,I65>L(+ + +)| c198>t|c201>t|a232>g,I78>V(+ - +)|c248>a,A83>D(- - -)|a254>g,N85>S(- - -)|c255>t,N85>S(- - -)|a258>t|a275>c,N92>T(- + -)|a277>g,S93>G(+ + -)|g291>a|t300>g|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)| 320 225 204 21 7 14 4 3 4 1 2 1 2 0 1 1 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 1 1 0 0 0 0 0 0 1 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 24 21 3 2 1 0 0 0 0 1 0 1 0 1 0 0 0 117 114 100 14 5 9 3 2 3 1 1 1 1 0 0 0 1 1 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,163-164(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,156-159(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tacc,189-192(CDR2)|tact,196-199(FR3)|tgcc,246-249(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcagtttcagtgactatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtagttctagtagtacccta tattatgcagactctgtgaagggccgattcaccgtctccagagacaatgacaagagttctctgtatctgcaaatgaccggcctgagagccgaagacacggcgacttattactgt gcgagagagtcgacgtgggaatttactcctgactcc ARESTWEFTPDS IGHV3-48 IGHD2-21 IGHJ4 ggattcagtttcagtgactatagc atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac attagtagttctagtagtacccta tattatgcagactctgtgaagggccgattcaccgtctccagagacaatgacaagagttctctgtatctgcaaatgaccggcctgagagccgaagacacggcgacttattactgt gcgagagagtcgacgtgggaatttactcctgactcc unmatched 21 213 12 9 7 11 5 10 5 2 9 5 JY8QFUQ01DC8OC 19366 productive (see comment) Homsap IGHV3-23*05 F 826 87.79 187/213 nt 87.32 186/213 nt Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD1-14*01 ORF 1 8 7 10 8.7.10 [1.17.38.11] CAKRPPGTIDRW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 63 of V-REGION: 3 nucleotides (from position 94 in the user submitted sequence), (do not cause frameshift) tctggattcacctttggcacctctgacatggcctgggtccgccaggttccaggggaggggctggagtgggtctcacacattgatatcagaggtgccacacagtataaagactccgtgaagggccggttcaccatctccagagacaattccaagagcactctatatctgcaaatgaacaccttgcgagccgaggacacggccgtatattactgtgcgaaacgcccgcccggcaccatagatcgctggggccagggaaccctggtcaccgtctcctcagcatcccctgaccagccccaaggtcttcccgctgngcctcgacagcaccccccgaagactgggaacgtggtcgtcgcatgcctggtccagggcttcttccccc NA unmatched, IGA2 43 63 [275, 277] a103>g,S35>G(+ + -)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|c113>a,A38>D(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g163>c,A55>H(- - -)|c164>a,A55>H(- - -)|t165>c,A55>H(- - -)|t169>g,Y57>D(- - -)|g173>t,S58>I(- - -)|t177>a,S59>R(- - -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|t196>c,Y66>Q(- - -)|c198>g,Y66>Q(- - -)|g202>a,A68>K(- - -)|c203>a,A68>K(- - -)|a254>g,N85>S(- - -)|g258>t|g261>a|g278>c,S93>T(+ - +)|c280>t|a283>c| a103>g,S35>G(+ + -)|g107>c,S36>T(+ - +)|a110>c,Y37>S(+ - -)|c113>a,A38>D(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|c134>t,A45>V(+ - +)|a142>g,K48>E(+ - -)|g163>c,A55>H(- - -)|c164>a,A55>H(- - -)|t165>c,A55>H(- - -)| t169>g,Y57>D(- - -)|g173>t,S58>I(- - -)|t177>a,S59>R(- - -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| t196>c,Y66>Q(- - -)|c198>g,Y66>Q(- - -)|g202>a,A68>K(- - -)|c203>a,A68>K(- - -)|a254>g,N85>S(- - -)|g258>t|g261>a|g278>c,S93>T(+ - +)|c280>t|a283>c| 318 219 193 26 4 22 5 2 2 2 2 3 0 1 5 1 2 1 78 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 0 4 1 0 0 0 1 1 0 0 1 0 0 0 51 51 44 7 0 7 2 0 1 1 0 1 0 0 2 0 0 0 30 21 16 5 0 5 1 0 0 0 0 0 0 1 1 0 1 1 117 114 104 10 4 6 1 2 1 1 1 1 0 0 1 1 1 0 6 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,186-187(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|agca,172-175(CDR2)|ggta,184-187(CDR2)|agca,190-193(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| tct ggattcacctttggcacctctgac atggcctgggtccgccaggttccaggggaggggctggagtgggtctcacac attgatatcagaggtgccaca cagtataaagactccgtgaagggccggttcaccatctccagagacaattccaagagcactctatatctgcaaatgaacaccttgcgagccgaggacacggccgtatattactgt gcgaaacgcccgcccggcaccatagatcgc AKRPPGTIDR IGHV3-23 IGHD1-14 IGHJ4 ggattcacctttggcacctctgac atggcctgggtccgccaggttccaggggaggggctggagtgggtctcacac attgatatcagaggtgccaca cagtataaagactccgtgaagggccggttcaccatctccagagacaattccaagagcactctatatctgcaaatgaacaccttgcgagccgaggacacggccgtatattactgt gcgaaacgcccgcccggcaccatagatcgc unmatched 26 210 11 15 4 15 7 11 4 0 13 9 JY8QFUQ01DCPGQ 4237 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*18 F or Homsap IGHV3-30-5*01 F 918 91.24 198/217 nt NA Homsap IGHJ4*02 F 204 91.67 44/48 nt Homsap IGHD5-12*01 F 3 8 8 13 8.8.13 [1.17.38.11] CAKGRGYNDYGLDYW in-frame + ctctggattcaccttcagtgactatggcatgcactgggtccgccaggctccaggcaaggggctggagtgggtgacagttattttatatgatggaagtagaaaatactatgcagactccgtgaagggccgattcgccatctccagagacgtttcgaggaacacgttgtatctgcagatgaatagcctgagacctgaggacacggctgtatactactgcgcgaaaggacgtggatataatgactacggcctcgactactggggccagggaaccctggtcaccgtctcctcagcctccacaagggcccatcggtctcccctggcacctcctccaagagcacctctgggggccacagcggccctgggctgcctggtcaaggactacttccccggaaccggtgacggtgtcgtggaactc NA unmatched, IGG1 64 56 [284] a106>g,S36>D(- - -)|g107>a,S36>D(- - -)|g160>a,A54>T(- - -)|a168>t|c170>t,S57>L(- - -)|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)|a229>g,T77>A(- - -)|a244>g,N82>V(- - -)|a245>t,N82>V(- - -)|c249>g|a251>g,K84>R(+ + +)|c259>t|a270>g|c276>t|g286>c,A96>P(- - -)|g303>a|t306>c|t312>c|g317>a,R106>K(+ + +)| a106>g,S36>D(- - -)|g107>a,S36>D(- - -)| g160>a,A54>T(- - -)| a168>t|c170>t,S57>L(- - -)|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)| a229>g,T77>A(- - -)|a244>g,N82>V(- - -)|a245>t,N82>V(- - -)|c249>g|a251>g,K84>R(+ + +)|c259>t|a270>g|c276>t|g286>c,A96>P(- - -)|g303>a|t306>c|t312>c| g317>a,R106>K(+ + +)|a320>g| 319 (320) 224 (225) 204 20 (21) 8 (9) 12 6 (7) 4 3 2 0 0 2 1 1 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 22 2 0 2 1 1 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 20 4 1 3 1 0 1 0 0 0 1 1 0 0 0 0 117 114 102 12 7 5 4 1 2 2 0 0 1 0 1 1 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 1 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtgactatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtgacagtt attttatatgatggaagtagaaaa tactatgcagactccgtgaagggccgattcgccatctccagagacgtttcgaggaacacgttgtatctgcagatgaatagcctgagacctgaggacacggctgtatactactgc gcgaaaggacgtggatataatgactacggcctcgactac AKGRGYNDYGLDY IGHV3-30 IGHD5-12 IGHJ4 ggattcaccttcagtgactatggc atgcactgggtccgccaggctccaggcaaggggctggagtgggtgacagtt attttatatgatggaagtagaaaa tactatgcagactccgtgaagggccgattcgccatctccagagacgtttcgaggaacacgttgtatctgcagatgaatagcctgagacctgaggacacggctgtatactactgc gcgaaaggacgtggatataatgactacggcctcgactac unmatched 19 213 14 5 6 8 8 11 7 1 6 5 JY8QFUQ01DEKWC 6425 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 927 92.17 200/217 nt NA Homsap IGHJ6*02 F 121 66.13 41/62 nt Homsap IGHD3-3*01 F 3 8 8 15 8.8.15 [1.17.38.11] CAKQTIFDYYYYGLDVW in-frame + ctctggattcatgtttagtagctttcccatggcctgggtccgccaggctccagggaaggggctggagtgggtctctagtattagtggtaggggtggtaacacatacttcgcagactccgtgaagggccggttcaacatctccagagacaattccaagaacacgatgtatttgcaaatgaacagcctgagagccgaggacacggccttatattactgtgcgaagcagacgattttcgactactactactacggtttggacgtctggggccaaggggaccacggtcaccgtctcctcagcatccccggaccagccccaaggtcttcctgctgagcctcgacagcaccccccgaagatgggaacgtggtccgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 50 68 [295, 287, 296] c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|c105>t|a110>t,Y37>F(- + -)|g112>c,A38>P(- - -)|a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a162>t|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|t177>g,S59>R(- - -)|g191>a,S64>N(- - -)|a200>t,Y67>F(- + -)|c230>a,T77>N(- + -)|c259>a,L87>M(+ + -)|c265>t|g301>t,V101>L(+ - +)| c86>t,T29>M(- - -)|c87>g,T29>M(- - -)|c105>t|a110>t,Y37>F(- + -)|g112>c,A38>P(- - -)| a118>g,S40>A(- + -)|g119>c,S40>A(- + -)|a162>t|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| t177>g,S59>R(- - -)|g191>a,S64>N(- - -)| a200>t,Y67>F(- + -)|c230>a,T77>N(- + -)|c259>a,L87>M(+ + -)|c265>t|g301>t,V101>L(+ - +)| a318>g|g319>c| 317 (320) 222 (225) 205 (206) 17 (19) 3 (5) 14 1 (2) 2 3 0 0 2 3 0 2 (3) 2 1 1 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 0 2 0 0 0 1 0 1 1 0 0 51 51 46 5 1 4 1 1 0 0 0 0 1 0 1 1 0 0 30 24 22 2 0 2 0 1 0 0 0 0 0 0 0 0 0 1 117 114 109 5 1 4 0 0 1 0 0 2 1 0 0 0 1 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 (1) 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatgtttagtagctttccc atggcctgggtccgccaggctccagggaaggggctggagtgggtctctagt attagtggtaggggtggtaacaca tacttcgcagactccgtgaagggccggttcaacatctccagagacaattccaagaacacgatgtatttgcaaatgaacagcctgagagccgaggacacggccttatattactgt gcgaagcagacgattttcgactactactactacggtttggacgtc AKQTIFDYYYYGLDV IGHV3-23 IGHD3-3 IGHJ6 ggattcatgtttagtagctttccc atggcctgggtccgccaggctccagggaaggggctggagtgggtctctagt attagtggtaggggtggtaacaca tacttcgcagactccgtgaagggccggttcaacatctccagagacaattccaagaacacgatgtatttgcaaatgaacagcctgagagccgaggacacggccttatattactgt gcgaagcagacgattttcgactactactactacggtttggacgtc unmatched 17 213 6 11 5 12 1 5 2 1 8 6 JY8QFUQ01DG2P7 6072 productive (see comment) Homsap IGHV1-3*01 F 956 94.81 201/212 nt 94.34 200/212 nt Homsap IGHJ4*02 F 177 85.42 41/48 nt Homsap IGHD2-8*01 F 2 8 7 9 8.7.9 [0.17.38.11] CARWNNDLDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 64 of V-REGION: 2 nucleotides (from position 96 in the user submitted sequence), (cause frameshift) tggatacaccttcagtacctatactatgaattgggtgcgccaggcccccggacaaaggcttgagtggatgggatggatcaacgctgaccttggcaacaaaatattcacagaagttccagggcagactcaccattaccagggacacatccgcgaacacagcctacatggagctgagcagcctgacatctgaagacacggctgtgtattactgtgcgagatggaacaatgaccttgactattggggccagggaaccctagtcactgtctcttcagcatcccctgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 79 [271, 272] c104>g,T35>S(+ - +)|g107>c,S36>T(+ - +)|g112>a,A38>T(- - -)|c118>a,H40>N(- - -)|g176>a,G59>D(- - -)|a184>c,N62>L(- - -)|a185>t,N62>L(- - -)|t189>c|g226>c,V76>L(+ - +)|g254>a,S85>N(- - -)|g284>c,R95>T(- - -)| c104>g,T35>S(+ - +)|g107>c,S36>T(+ - +)|g112>a,A38>T(- - -)| c118>a,H40>N(- - -)| g176>a,G59>D(- - -)|a184>c,N62>L(- - -)|a185>t,N62>L(- - -)|t189>c| g226>c,V76>L(+ - +)|g254>a,S85>N(- - -)|g284>c,R95>T(- - -)| g319>t|a320>g| 318 (320) 218 (220) 207 11 (13) 1 (3) 10 0 (1) 3 0 1 1 1 1 0 3 1 0 (1) 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 1 0 0 0 0 0 0 1 1 0 0 51 51 50 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 30 22 18 4 1 3 0 1 0 1 1 0 1 0 0 0 0 0 117 114 111 3 0 3 0 1 0 0 0 0 0 0 2 0 0 0 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 0 0 0 0 (1) 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,169-170(CDR2)|aa,184-185(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|aa,197-198(FR3)|ta,199-200(FR3)|aa,208-209(FR3)|ta,234-235(FR3)|ta,262-263(FR3)|aa,290-291(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,11-12(FR1)|tt,62-63(FR1)|tt,75-76(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|tt,120-121(FR2)|tt,149-150(FR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,201-202(FR3)|tt,211-212(FR3)|tt,233-234(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggct,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tacc,234-237(FR3)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcagtacctatact atgaattgggtgcgccaggcccccggacaaaggcttgagtggatgggatgg atcaacgctgaccttggcaaca aaatattcacagaagttccagggcagactcaccattaccagggacacatccgcgaacacagcctacatggagctgagcagcctgacatctgaagacacggctgtgtattactgt gcgagatggaacaatgaccttgactat ARWNNDLDY IGHV1-3 IGHD2-8 IGHJ4 ggatacaccttcagtacctatact atgaattgggtgcgccaggcccccggacaaaggcttgagtggatgggatgg atcaacgctgaccttggcaaca aaatattcacagaagttccagggcagactcaccattaccagggacacatccgcgaacacagcctacatggagctgagcagcctgacatctgaagacacggctgtgtattactgt gcgagatggaacaatgaccttgactat unmatched 11 211 4 7 3 8 1 3 0 1 4 6 JY8QFUQ01DG5KX 8750 productive Homsap IGHV3-66*02 F, or Homsap IGHV3-66*03 F 867 89.72 192/214 nt NA Homsap IGHJ4*02 F (see comment) 195 89.58 43/48 nt Homsap IGHD6-25*01 F 1 8 7 11 8.7.11 [1.17.38.11] CARDGRGGDLDSW in-frame + Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) gtctgggttctccgtcagtttcaactacatgagctgggtccgccaggctccagggaaggggctggagtgggtctcagttatctatgccgatggaagtacattctatgcagactccgtgaagggccgattcatcatctccagagacaattcaaagaacacgctcaatcttcaaatgaatagtttgagagttgacgacacggctgtgtattactgtgcgagagatgggcgcggcggtgaccttgactcctggggccagggaaccctggtcaccgtctcctcagcatccccgcagcagcccctaaggtcttccgctgagcctcgacacgcacccccaagcatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 43 42 [280] c75>g|a81>g|a85>t,T29>S(+ - +)|a106>t,S36>F(- - -)|g107>t,S36>F(- - -)|t168>c|a172>g,S58>A(- + -)|g173>c,S58>A(- + -)|g176>a,G59>D(- - -)|t189>a|c192>t|a197>t,Y66>F(- + -)|c201>t|c230>t,T77>I(- - -)|c249>a|g261>c|t262>a,Y88>N(- - -)|c276>t|c279>t|c280>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| c75>g| a81>g|a85>t,T29>S(+ - +)|a106>t,S36>F(- - -)|g107>t,S36>F(- - -)| t168>c|a172>g,S58>A(- + -)|g173>c,S58>A(- + -)|g176>a,G59>D(- - -)|t189>a|c192>t| a197>t,Y66>F(- + -)|c201>t|c230>t,T77>I(- - -)|c249>a|g261>c|t262>a,Y88>N(- - -)|c276>t|c279>t|c280>t|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)| 318 220 198 22 11 11 2 1 7 1 0 1 3 2 3 1 1 0 78 4 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 36 24 20 4 1 3 1 0 0 0 0 0 2 0 0 0 1 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 15 6 3 3 1 1 1 1 0 0 0 1 1 0 0 0 117 114 103 11 6 5 0 0 6 0 0 1 1 1 2 0 0 0 6 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|aa,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|ta,105-106(CDR1)|ta,112-113(CDR1)|tt,164-165(FR2)|tt,167-168(CDR2)|ta,169-170(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|tt,266-267(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agtt,162-165(FR2)|ggta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|aact,109-112(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| gtct gggttctccgtcagtttcaactac atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagtt atctatgccgatggaagtaca ttctatgcagactccgtgaagggccgattcatcatctccagagacaattcaaagaacacgctcaatcttcaaatgaatagtttgagagttgacgacacggctgtgtattactgt gcgagagatgggcgcggcggtgaccttgactcc ARDGRGGDLDS IGHV3-66 IGHD6-25 IGHJ4 gggttctccgtcagtttcaactac atgagctgggtccgccaggctccagggaaggggctggagtgggtctcagtt atctatgccgatggaagtaca ttctatgcagactccgtgaagggccgattcatcatctccagagacaattcaaagaacacgctcaatcttcaaatgaatagtttgagagttgacgacacggctgtgtattactgt gcgagagatgggcgcggcggtgaccttgactcc unmatched 21 210 11 10 8 13 3 8 6 4 5 6 JY8QFUQ01DG6GC 13953 productive Homsap IGHV3-30*15 F 945 93.09 202/217 nt NA Homsap IGHJ4*02 F 231 97.92 47/48 nt Homsap IGHD5-24*01 ORF 1 8 8 16 8.8.16 [1.17.38.11] CARDELPWDTIEYYFDYW in-frame + ctctggattcacctttagtagatattccatgcactgggtccgccaggctccaggcaaggggctagagtgggtggcacttatatcatacgatggaagtagaagaatctacgcagactccgtgaagggccgattcaccatctccagagacacttccaagaacacggtgtatctgcaaatgagtagcctgagacctgaggacacggctgtgtattactgtgcgagagatgagctgccttgggatacaatagaatactactttgactattggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA unmatched, IGA2 57 89 [296, 297] c90>t|c108>a,S36>R(- - -)|g112>t,A38>S(- + -)|t114>c,A38>S(- + -)|g163>c,V55>L(+ - +)|t174>c|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)|a194>g,K65>R(+ + +)|t196>a,Y66>I(- - -)|a197>t,Y66>I(- - -)|a245>c,N82>T(- + -)|c259>g,L87>V(+ - +)|c276>t|g286>c,A96>P(- - -)| c90>t|c108>a,S36>R(- - -)|g112>t,A38>S(- + -)|t114>c,A38>S(- + -)| g163>c,V55>L(+ - +)| t174>c|a191>g,N64>R(+ - -)|t192>a,N64>R(+ - -)|a194>g,K65>R(+ + +)| t196>a,Y66>I(- - -)|a197>t,Y66>I(- - -)|a245>c,N82>T(- + -)|c259>g,L87>V(+ - +)|c276>t|g286>c,A96>P(- - -)| 320 225 210 15 3 12 2 0 2 2 1 1 1 2 2 1 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 20 4 1 3 0 0 1 1 0 1 0 0 0 0 1 0 51 51 50 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 30 24 20 4 1 3 2 0 0 1 0 0 0 1 0 0 0 0 117 114 108 6 1 5 0 0 1 0 1 0 1 1 1 1 0 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|aa,142-143(FR2)|ta,149-150(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,114-115(CDR1)|ta,149-150(FR2)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agca,274-277(FR3)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgct,111-114(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctttagtagatattcc atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcactt atatcatacgatggaagtagaaga atctacgcagactccgtgaagggccgattcaccatctccagagacacttccaagaacacggtgtatctgcaaatgagtagcctgagacctgaggacacggctgtgtattactgt gcgagagatgagctgccttgggatacaatagaatactactttgactat ARDELPWDTIEYYFDY IGHV3-30 IGHD5-24 IGHJ4 ggattcacctttagtagatattcc atgcactgggtccgccaggctccaggcaaggggctagagtgggtggcactt atatcatacgatggaagtagaaga atctacgcagactccgtgaagggccgattcaccatctccagagacacttccaagaacacggtgtatctgcaaatgagtagcctgagacctgaggacacggctgtgtattactgt gcgagagatgagctgccttgggatacaatagaatactactttgactat unmatched 15 213 6 9 2 7 4 8 1 2 6 6 JY8QFUQ01DHXHT 21460 productive Homsap IGHV4-39*02 F 920 90.95 201/221 nt NA Homsap IGHJ4*02 F 168 83.33 40/48 nt Homsap IGHD4-17*01 F 3 10 7 14 10.7.14 [1.17.38.11] CARPGARETNNAWVDW in-frame + tctctggtggctccgtcagtaggagtgcctactactggggctggatccgccagcccccagggaaggggctggagtggattgggaccatctattatagtgggaccacatactccaatccgtccctcaagactcgagtcaccatgtccttggacacgtccaagaaccacatctccctgaagctgaattctgtgaccgccgcagacacggctgtttattactgtgcgagacctggggctagggagactaataacgcctgggtcgactggggccagggcaccctggtcaccgtctcctcggcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggccacagcgccctgggctgcctggtcaaggactcacttccccgaaccggtgacggtgtgcgtgaactca NA unmatched, IGG1 57 81 [295] a88>g,I30>V(+ - +)|c93>t|t102>g,S34>R(- - -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|t108>c,S36>A(- + -)|g164>c,S55>T(+ - +)|t165>c,S55>T(+ - +)|g191>c,S64>T(+ - +)|c195>a|a200>c,Y67>S(+ - -)|c204>t|g221>c,S74>T(+ - +)|a234>g,I78>M(+ + -)|g238>t,V80>L(+ - +)|a240>g,V80>L(+ - +)|t259>a,F87>I(+ - -)|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)|g303>t| a88>g,I30>V(+ - +)|c93>t|t102>g,S34>R(- - -)|a106>g,S36>A(- + -)|g107>c,S36>A(- + -)|t108>c,S36>A(- + -)| g164>c,S55>T(+ - +)|t165>c,S55>T(+ - +)| g191>c,S64>T(+ - +)|c195>a| a200>c,Y67>S(+ - -)|c204>t|g221>c,S74>T(+ - +)|a234>g,I78>M(+ + -)|g238>t,V80>L(+ - +)|a240>g,V80>L(+ - +)|t259>a,F87>I(+ - -)|g275>a,S92>N(- - -)|c276>t,S92>N(- - -)|g303>t| g319>c|a320>c| 318 (320) 227 (229) 207 20 (22) 4 (6) 16 4 1 3 2 1 (2) 1 0 1 4 (5) 0 2 1 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 24 6 1 5 2 0 1 1 0 0 0 0 1 0 0 1 51 51 49 2 0 2 0 0 0 1 0 0 0 0 1 0 0 0 30 21 19 2 1 1 0 0 0 0 0 1 0 0 1 0 0 0 117 114 104 10 2 8 2 1 2 0 1 0 0 1 1 0 2 0 6 (8) 6 6 0 (2) 0 (2) 0 0 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 aa,40-41(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,102-103(CDR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|ggct,82-85(CDR1)|agta,100-103(CDR1)|agtt,106-109(CDR1)|ggct,118-121(FR2)|ggct,146-149(FR2)|agta,163-166(FR2-CDR2)|agca,190-193(CDR2)|agct,269-272(FR3)|agct,274-277(FR3)|ggct,297-300(FR3)| agct,2-5(FR1)|agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcagtaggagtgcctactac tggggctggatccgccagcccccagggaaggggctggagtggattgggacc atctattatagtgggaccaca tactccaatccgtccctcaagactcgagtcaccatgtccttggacacgtccaagaaccacatctccctgaagctgaattctgtgaccgccgcagacacggctgtttattactgt gcgagacctggggctagggagactaataacgcctgggtcgac ARPGARETNNAWVD IGHV4-39 IGHD4-17 IGHJ4 ggtggctccgtcagtaggagtgcctactac tggggctggatccgccagcccccagggaaggggctggagtggattgggacc atctattatagtgggaccaca tactccaatccgtccctcaagactcgagtcaccatgtccttggacacgtccaagaaccacatctccctgaagctgaattctgtgaccgccgcagacacggctgtttattactgt gcgagacctggggctagggagactaataacgcctgggtcgac unmatched 20 216 10 10 4 11 6 9 2 2 10 6 JY8QFUQ01DI39D 20906 productive (see comment) Homsap IGHV1-24*01 F 993 96.26 206/214 nt 95.79 205/214 nt Homsap IGHJ5*02 F 141 77.08 37/48 nt Homsap IGHD5-18*01 F 1 8 8 7 8.8.7 [0.17.38.11] CAIGAMATW in-frame - Potentially, because of detected insertion/deletion in FR2-IMGT at codon 48 of V-REGION: 'A' (1 nucleotides at position 54 to 54 in the user submitted sequence), (cause frameshift); cggaaaaaccctcactgaagtatccatgcactgggtgcgacaggctcctggaaAaagggcttgagtggatgggaggatttgatcctgaagatggtgaaataatctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgtgcaataggagctatggccacctggggccagggaaccctggtcaccgtcgactcagcatccccgaccagccccaaggtcttcccgctgagcctctgcagcaccccagccagatggggaacgtggtcatcgccctgcctggtccagggcttcttccccccag NA unmatched, IGA1 64 84 [267, 269] t82>a,Y28>K(- - -)|c84>a,Y28>K(- - -)|t109>g,L37>V(+ - +)|t165>a|c194>t,T65>I(- - -)|g226>a,V76>I(+ - +)|a232>g,M78>V(+ - -)|g291>a|c317>t,T106>I(- - -)| t82>a,Y28>K(- - -)|c84>a,Y28>K(- - -)|t109>g,L37>V(+ - +)| t165>a| c194>t,T65>I(- - -)| g226>a,V76>I(+ - +)|a232>g,M78>V(+ - -)|g291>a| c317>t,T106>I(- - -)|a320>g| 319 (320) 221 (222) 212 9 (10) 2 (3) 7 1 (2) 2 2 0 0 1 0 2 0 0 0 1 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 21 3 0 3 0 0 0 0 0 1 0 1 0 0 0 1 51 51 50 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 30 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 117 114 111 3 1 2 1 2 0 0 0 0 0 0 0 0 0 0 7 (8) 7 6 1 (2) 0 (1) 1 0 (1) 0 1 0 0 0 0 0 0 0 0 0 ta,14-15(FR1)|aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|aa,107-108(CDR1)|ta,110-111(CDR1)|aa,141-142(FR2)|aa,143-144(FR2)|aa,176-177(CDR2)|aa,191-192(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,249-250(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| ta,14-15(FR1)|tt,74-75(FR1)|ta,82-83(CDR1)|tt,109-110(CDR1)|tt,149-150(FR2)|tt,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,249-250(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggta,12-15(FR1)|ggct,24-27(FR1)|ggtt,72-75(FR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|ggtt,163-166(FR2-CDR2)|agtt,209-212(FR3)|ggca,220-223(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,258-261(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| c ggaaaaaccctcactgaagtatcc atgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggagga tttgatcctgaagatggtgaaata atctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcaataggagctatggccacc AIGAMAT IGHV1-24 IGHD5-18 IGHJ5 ggaaaaaccctcactgaagtatcc atgcactgggtgcgacaggctcctggaaaagggcttgagtggatgggagga tttgatcctgaagatggtgaaata atctacgcacagaagttccagggcagaatcaccgtgaccgaggacacatctacagacacagcctacatggagctgagcagcctgagatctgaagacacggccgtgtattactgt gcaataggagctatggccacc unmatched 8 213 4 4 3 4 1 4 2 0 2 4 JY8QFUQ01DIBNC 10197 productive (see comment) Homsap IGHV4-61*01 F 1100 100 221/221 nt 99.1 219/221 nt Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD5-12*01 F 3 10 7 20 10.7.20 [1.17.38.11] CARVSGYAYGRYGDYLRGVDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT at codon 63 of V-REGION: 'G' (1 nucleotides at position 99 to 99 in the user submitted sequence), (cause frameshift);in FR3-IMGT at codon 68 of V-REGION: 'C' (1 nucleotides at position 117 to 117 in the user submitted sequence), (cause frameshift); tctctggtggctccgtcagcagtggtagttactactggagctggatccggcagcccccagggaagggactggagtggattgggtatatctattacagtGgggagcaccaactacaaCcccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgtgcgagggttagtggctacgcgtacggacgctacggtgactatttgcgcggggttgactactggggccagggaaccctggtcaccgtctcctcagcctccaccaaggggcccctatcggtcttcccccgtggcaccctcctccaagagcacctctgggggccacagcggcccctgggctgcctggtcaaggactacttccccagaaccggtgacggtgtcgtggaactcag NA unmatched, IGG1 79 69 [319, 315] a318>g|a320>t| 317 (320) 226 (229) 226 (227) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 21 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117 114 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (1) 0 0 0 0 0 0 (1) 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|aa,142-143(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|ta,172-173(CDR2)|aa,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,105-106(CDR1)|tt,108-109(CDR1)|ta,112-113(CDR1)|tt,158-159(FR2)|ta,163-164(FR2)|ta,165-166(FR2-CDR2)|ta,169-170(CDR2)|tt,171-172(CDR2)|ta,199-200(FR3)|ta,233-234(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,82-85(CDR1)|ggta,103-106(CDR1)|agct,118-121(FR2)|ggca,128-131(FR2)|ggta,161-164(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|tact,109-112(CDR1)|agct,118-121(FR2)|agcc,131-134(FR2)|aact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct ggtggctccgtcagcagtggtagttactac tggagctggatccggcagcccccagggaagggactggagtggattgggtat atctattacagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgt gcgagggttagtggctacgcgtacggacgctacggtgactatttgcgcggggttgactac ARVSGYAYGRYGDYLRGVDY IGHV4-61 IGHD5-12 IGHJ4 ggtggctccgtcagcagtggtagttactac tggagctggatccggcagcccccagggaagggactggagtggattgggtat atctattacagtgggagcacc aactacaacccctccctcaagagtcgagtcaccatatcagtagacacgtccaagaaccagttctccctgaagctgagctctgtgaccgctgcggacacggccgtgtattactgt gcgagggttagtggctacgcgtacggacgctacggtgactatttgcgcggggttgactac unmatched 0 216 0 0 0 0 0 0 0 0 0 0 JY8QFUQ01DJFIZ 9776 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23*05 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F (see comment) 693 80.18 174/217 nt NA Homsap IGHJ5*02 F 210 90.2 46/51 nt Homsap IGHD5-12*01 F 3 8 8 15 8.8.15 [1.17.38.11] CAKSPDSGYDSNWLDPW in-frame + Low V-REGION identity (80,18% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release ctctggattcaacttggcgaagttcgccatgagctgggtccgccaggctcctgggaaggggctggagtgggtctcagagatcagtggctccggtagtaaagtcggatatgcggagtccgtgaagggccgattcaccatctccaaagacaattccaagaacacattgtacttgcaaatgaccgacctgagacccggcgacacggccatttattactgtgcgaaaagtcctgatagtggctacgattctaactggctcgacccctggggcctgggaaccctggtcaccgtctcgtcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggggccacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcag NA unmatched, IGG1 64 88 [294] c86>a,T29>N(- + -)|t90>g,F30>L(+ - -)|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|c105>g,S35>A(- + -)|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a110>t,Y37>F(- + -)|t111>c,Y37>F(- + -)|a138>t|c164>a,A55>E(- - -)|t165>g,A55>E(- - -)|t168>c|t174>c|a175>t|g176>c|t177>c|g187>a,G63>S(+ + -)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|a195>c,T65>V(- - -)|t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>a,Y66>G(+ - -)|c201>t|a204>g|c207>g,D69>E(+ - +)|g225>a|g239>a,R80>K(+ + +)|g258>a|c259>t|t264>c|c265>t|a275>c,N92>T(- + -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g286>c,A96>P(- - -)|a290>g,E97>G(- - -)|g291>c,E97>G(- - -)|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| c86>a,T29>N(- + -)|t90>g,F30>L(+ - -)|a103>g,S35>A(- + -)|g104>c,S35>A(- + -)|c105>g,S35>A(- + -)|g107>a,S36>K(- - -)|c108>g,S36>K(- - -)|a110>t,Y37>F(- + -)|t111>c,Y37>F(- + -)| a138>t|c164>a,A55>E(- - -)|t165>g,A55>E(- - -)| t168>c|t174>c|a175>t|g176>c|t177>c|g187>a,G63>S(+ + -)|g191>a,S64>K(- - -)|c192>a,S64>K(- - -)|a193>g,T65>V(- - -)|c194>t,T65>V(- - -)|a195>c,T65>V(- - -)| t196>g,Y66>G(+ - -)|a197>g,Y66>G(+ - -)|c198>a,Y66>G(+ - -)|c201>t|a204>g|c207>g,D69>E(+ - +)|g225>a|g239>a,R80>K(+ + +)|g258>a|c259>t|t264>c|c265>t|a275>c,N92>T(- + -)|a277>g,S93>D(- - -)|g278>a,S93>D(- - -)|g286>c,A96>P(- - -)|a290>g,E97>G(- - -)|g291>c,E97>G(- - -)|g301>a,V101>I(+ - +)|a303>t,V101>I(+ - +)| g319>a|a320>g| 318 (320) 223 (225) 180 43 (45) 13 (15) 30 6 (7) 8 (9) 4 5 2 4 4 0 4 3 0 3 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 15 9 0 9 1 1 0 1 0 1 1 0 1 2 0 1 51 51 48 3 1 2 0 0 0 0 0 1 1 0 0 0 0 1 30 24 13 11 5 6 1 2 1 3 1 1 1 0 1 0 0 0 117 114 94 20 7 13 4 5 3 1 1 1 1 0 2 1 0 1 6 (8) 6 6 0 (2) 0 (2) 0 0 (1) 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaacttggcgaagttcgcc atgagctgggtccgccaggctcctgggaaggggctggagtgggtctcagag atcagtggctccggtagtaaagtc ggatatgcggagtccgtgaagggccgattcaccatctccaaagacaattccaagaacacattgtacttgcaaatgaccgacctgagacccggcgacacggccatttattactgt gcgaaaagtcctgatagtggctacgattctaactggctcgacccc AKSPDSGYDSNWLDP IGHV3-23 IGHD5-12 IGHJ5 ggattcaacttggcgaagttcgcc atgagctgggtccgccaggctcctgggaaggggctggagtgggtctcagag atcagtggctccggtagtaaagtc ggatatgcggagtccgtgaagggccgattcaccatctccaaagacaattccaagaacacattgtacttgcaaatgaccgacctgagacccggcgacacggccatttattactgt gcgaaaagtcctgatagtggctacgattctaactggctcgacccc unmatched 43 213 23 20 12 23 11 20 8 5 15 15 JY8QFUQ01DLDLD 6702 productive Homsap IGHV1-46*01 F, or Homsap IGHV1-46*03 F 1002 96.73 207/214 nt NA Homsap IGHJ4*02 F 195 89.58 43/48 nt Homsap IGHD6-13*01 F 3 8 8 11 8.8.11 [0.17.38.11] CARGQQLRDFYYW in-frame + tggatacaccttcaccagctactatatacactgggtgcgacaggcccctggacaagggcttgagtggatgggaataatcgaccctagtggtggtgccacaagctacgcacagcagttccagggcagagtcaccatgaccagggacacgtccacgagcacagtctatatggagctgagcagcctgagatctgacgacacggccgtgtattactgtgcgaggggacagcagcttcgagacttttactactggggccagggaaccctggtcaccgtcccctcagcatcccctgaccagcccctaaggtcttcccgctgacgcctctgcagcacccagccagatgggaacgtggtcatcgcctgcctggtccagggcttcttccccc NA unmatched, IGA1 86 68 [281] g117>a,M39>I(+ + -)|a169>g,N57>D(+ + -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)|a208>c,K70>Q(+ - -)|c264>t|g291>c,E97>D(+ - +)| g117>a,M39>I(+ + -)| a169>g,N57>D(+ + -)|a190>g,S64>A(- + -)|g191>c,S64>A(- + -)| a208>c,K70>Q(+ - -)|c264>t|g291>c,E97>D(+ - +)| a318>g|a320>g| 317 (320) 219 (222) 212 (213) 7 (9) 1 (3) 6 2 (4) 1 1 0 1 0 0 0 2 0 0 0 78 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 0 3 2 0 0 0 0 0 0 0 1 0 0 0 117 114 111 3 1 2 0 0 1 0 1 0 0 0 1 0 0 0 5 (8) 5 5 (6) 0 (2) 0 (2) 0 0 (2) 0 0 0 0 0 0 0 0 0 0 0 aa,37-38(FR1)|aa,40-41(FR1)|aa,58-59(FR1)|aa,70-71(FR1)|ta,82-83(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|aa,143-144(FR2)|aa,162-163(FR2)|ta,164-165(FR2)|aa,169-170(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,208-209(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,62-63(FR1)|ta,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,112-113(CDR1)|ta,114-115(CDR1)|tt,149-150(FR2)|ta,164-165(FR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,211-212(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,24-27(FR1)|ggtt,60-63(FR1)|ggca,72-75(FR1)|agct,106-109(CDR1)|ggct,146-149(FR2)|ggta,187-190(CDR2)|agct,196-199(FR3)|agtt,209-212(FR3)|ggca,220-223(FR3)|agca,253-256(FR3)|agct,269-272(FR3)|agca,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agct,106-109(CDR1)|aacc,169-172(CDR2)|agct,196-199(FR3)|agct,269-272(FR3)|agcc,277-280(FR3)|tact,307-310(FR3)| t ggatacaccttcaccagctactat atacactgggtgcgacaggcccctggacaagggcttgagtggatgggaata atcgaccctagtggtggtgccaca agctacgcacagcagttccagggcagagtcaccatgaccagggacacgtccacgagcacagtctatatggagctgagcagcctgagatctgacgacacggccgtgtattactgt gcgaggggacagcagcttcgagacttttactac ARGQQLRDFYY IGHV1-46 IGHD6-13 IGHJ4 ggatacaccttcaccagctactat atacactgggtgcgacaggcccctggacaagggcttgagtggatgggaata atcgaccctagtggtggtgccaca agctacgcacagcagttccagggcagagtcaccatgaccagggacacgtccacgagcacagtctatatggagctgagcagcctgagatctgacgacacggccgtgtattactgt gcgaggggacagcagcttcgagacttttactac unmatched 7 213 4 3 2 4 2 3 1 0 3 3 JY8QFUQ01DMF0A 6101 productive (see comment) Homsap IGHV4-55*03 P (see comment) 608 75.58 164/217 nt NA Homsap IGHJ4*02 F (see comment) 141 77.08 37/48 nt Homsap IGHD3-16*01 F 3 10 7 16 10.7.16 [1.17.38.11] CARESRNSFGGPTGLDSW in-frame + however the closest V is a pseudogene The submitted sequence and the closest germline V-GENE allele show different CDR1-IMGT amino acid lengths (10 AA in JY8QFUQ01DMF0A ; 9 AA in Homsap_IGHV4-55*03), and low V-REGION identity (75,58% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release Other possibilities: Homsap_IGHJ5*02 (highest number of consecutive identical nucleotides) tctctgttgacgccataagcgacctcggttatttctgggcctgggtccgccagcccgccgcgaagggactggagtggatcggacatgcccttggtgatggatataccgaatacaaccccgccctagagagtcgaatcaccgtgtcagtggacaagtccaagaaccagttttccctgacgttggagtccgtgaccgccgcagacacggccacttatttctgtgcgagggaaagtcggaattcatttggcgggccgacaggccttgattcctggggccagggaattgtggtcatcgtctcctcagcatccccggaccagccccaaggtcttcccgctgagcctctgcagcacccagccagatggacgtgtcctcgcctgcctggtccagggcttctccc NA unmatched, IGA1 79 68 [301] g80>t,G27>V(- - -)|t85>g,S29>A(- + -)|c90>a|a103>c,S35>L(- - -)|g104>t,S35>L(- - -)|t105>c,S35>L(- - -)|a109>t,N37>Y(- - -)|c111>t,N37>Y(- - -)|g113>t,W38>F(+ + -)|g114>c,W38>F(+ + -)|a117>g,*39>W(- - -)|a118>g,I40>A(+ - +)|t119>c,I40>A(+ - +)|c136>g,P46>A(- - -)|a138>c,P46>A(- - -)|g140>c,G47>A(- + -)|g147>a|t159>c|g162>a|g163>c,E55>H(- + -)|a165>t,E55>H(- + -)|a166>g,I56>A(+ - +)|t167>c,I56>A(+ - +)|a170>t,H57>L(- - -)|c172>g,H58>G(+ - -)|a173>g,H58>G(+ - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g189>a|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|c192>t,S64>Y(+ - -)|t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|g207>c|t208>g,S70>A(- + -)|c213>a|a214>g,K72>E(+ - -)|a232>g,M78>V(+ - -)|a240>g|c245>a,T82>K(- - -)|c261>t|a269>c,K90>T(- - -)|c271>t|a274>g,S92>E(- - -)|g275>a,S92>E(- - -)|c276>g,S92>E(- - -)|t279>c|g291>a|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|a308>t,Y103>F(- + -)| g80>t,G27>V(- - -)|t85>g,S29>A(- + -)|c90>a|a103>c,S35>L(- - -)|g104>t,S35>L(- - -)|t105>c,S35>L(- - -)|a109>t,N37>Y(- - -)|c111>t,N37>Y(- - -)|g113>t,W38>F(+ + -)|g114>c,W38>F(+ + -)| a117>g,*39>W(- - -)|a118>g,I40>A(+ - +)|t119>c,I40>A(+ - +)|c136>g,P46>A(- - -)|a138>c,P46>A(- - -)|g140>c,G47>A(- + -)|g147>a|t159>c|g162>a|g163>c,E55>H(- + -)|a165>t,E55>H(- + -)| a166>g,I56>A(+ - +)|t167>c,I56>A(+ - +)|a170>t,H57>L(- - -)|c172>g,H58>G(+ - -)|a173>g,H58>G(+ - -)|a175>g,S59>D(- - -)|g176>a,S59>D(- - -)|g189>a|a190>t,S64>Y(+ - -)|g191>a,S64>Y(+ - -)|c192>t,S64>Y(+ - -)| t196>g,Y66>E(- - -)|c198>a,Y66>E(- - -)|g207>c|t208>g,S70>A(- + -)|c213>a|a214>g,K72>E(+ - -)|a232>g,M78>V(+ - -)|a240>g|c245>a,T82>K(- - -)|c261>t|a269>c,K90>T(- - -)|c271>t|a274>g,S92>E(- - -)|g275>a,S92>E(- - -)|c276>g,S92>E(- - -)|t279>c|g291>a|g301>a,V101>T(- - -)|t302>c,V101>T(- - -)|g303>t,V101>T(- - -)|a308>t,Y103>F(- + -)| 311 220 167 53 12 41 9 8 4 6 3 4 5 0 4 3 4 3 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 30 20 10 1 9 0 0 1 1 1 1 1 0 1 0 3 1 51 51 40 11 3 8 2 2 0 2 1 0 1 0 2 1 0 0 30 21 10 11 1 10 3 3 1 1 0 0 2 0 0 1 0 0 116 114 92 21 7 14 4 3 2 2 1 3 1 0 1 1 1 2 0 aa,40-41(FR1)|ta,108-109(CDR1)|aa,117-118(FR2)|aa,142-143(FR2)|aa,164-165(FR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,202-203(FR3)|aa,214-215(FR3)|aa,225-226(FR3)|ta,239-240(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|aa,268-269(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,45-46(FR1)|ta,108-109(CDR1)|tt,158-159(FR2)|ta,174-175(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|ta,239-240(FR3)|tt,259-260(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggta,106-109(CDR1)|ggct,146-149(FR2)|agca,190-193(CDR2)|agta,237-240(FR3)|agtt,257-260(FR3)|agct,269-272(FR3)|agct,274-277(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|aact,109-112(CDR1)|agcc,131-134(FR2)|tact,196-199(FR3)|aacc,202-205(FR3)|aacc,253-256(FR3)|agct,269-272(FR3)|agct,274-277(FR3)|tact,307-310(FR3)| tctct gttgacgccataagcgacctcggttatttc tgggcctgggtccgccagcccgccgcgaagggactggagtggatcggacat gcccttggtgatggatatacc gaatacaaccccgccctagagagtcgaatcaccgtgtcagtggacaagtccaagaaccagttttccctgacgttggagtccgtgaccgccgcagacacggccacttatttctgt gcgagggaaagtcggaattcatttggcgggccgacaggccttgattcc ARESRNSFGGPTGLDS IGHV4-55 IGHD3-16 IGHJ4 gttgacgccataagcgacctcggttatttc tgggcctgggtccgccagcccgccgcgaagggactggagtggatcggacat gcccttggtgatggatatacc gaatacaaccccgccctagagagtcgaatcaccgtgtcagtggacaagtccaagaaccagttttccctgacgttggagtccgtgaccgccgcagacacggccacttatttctgt gcgagggaaagtcggaattcatttggcgggccgacaggccttgattcc unmatched 53 216 27 26 12 27 15 26 10 2 22 19 JY8QFUQ01DOVL5 8007 productive Homsap IGHV3-30*03 F, or Homsap IGHV3-30*04 F or Homsap IGHV3-30*14 F or Homsap IGHV3-30*18 F or Homsap IGHV3-30-3*03 F or Homsap IGHV3-30-5*01 F 945 93.09 202/217 nt NA Homsap IGHJ6*02 F (see comment) 193 79.03 49/62 nt Homsap IGHD5-24*01 ORF 3 8 8 18 8.8.18 [1.17.38.11] CARKGPRRDGSLQQCDMDVW in-frame + Other possibilities: Homsap_IGHJ4*03 (shorter alignment but highest percentage of identity) ctctggattcatcttcagcaaccttgcgatgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaattatatcatatgatggaggtattaagtactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctacaaatgaacaacctgagacttgaggacacggctgtgtattactgtgcgagaaaaggccccagaagagatggctccctacagcaatgcgatatggacgtctggggccaagggaccacgatcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 89 [302, 303] c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)|t109>c,Y37>L(- - -)|a110>t,Y37>L(- - -)|g113>c,G38>A(- + -)|c114>g,G38>A(- + -)|g163>a,V55>I(+ - +)|a187>g,S63>G(+ + -)|a191>t,N64>I(- - -)|a195>g|g267>a|g278>a,S93>N(- - -)|g286>c,A96>L(+ - +)|c287>t,A96>L(+ - +)| c86>t,T29>I(- - -)|t105>c|g107>a,S36>N(- - -)|t109>c,Y37>L(- - -)|a110>t,Y37>L(- - -)|g113>c,G38>A(- + -)|c114>g,G38>A(- + -)| g163>a,V55>I(+ - +)| a187>g,S63>G(+ + -)|a191>t,N64>I(- - -)|a195>g| g267>a|g278>a,S93>N(- - -)|g286>c,A96>L(+ - +)|c287>t,A96>L(+ - +)| g319>a| 318 (320) 223 (225) 208 (209) 15 (16) 3 (4) 12 2 4 (5) 2 2 0 0 2 0 2 1 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 1 6 0 1 1 2 0 0 1 0 1 1 0 0 51 51 50 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 30 24 21 3 1 2 2 0 0 0 0 0 1 0 0 0 0 0 117 114 110 4 1 3 0 2 1 0 0 0 0 0 1 0 0 0 6 (8) 6 6 (7) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|aa,186-187(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|aa,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,164-165(FR2)|ta,167-168(CDR2)|ta,172-173(CDR2)|ta,189-190(CDR2)|ta,192-193(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|ggca,112-115(CDR1)|ggct,132-135(FR2)|ggca,139-142(FR2)|ggct,146-149(FR2)|ggca,159-162(FR2)|agta,187-190(CDR2)|agct,285-288(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tact,196-199(FR3)|agcc,277-280(FR3)|agct,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagcaaccttgcg atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatt atatcatatgatggaggtattaag tactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctacaaatgaacaacctgagacttgaggacacggctgtgtattactgt gcgagaaaaggccccagaagagatggctccctacagcaatgcgatatggacgtc ARKGPRRDGSLQQCDMDV IGHV3-30 IGHD5-24 IGHJ6 ggattcatcttcagcaaccttgcg atgcactgggtccgccaggctccaggcaaggggctggagtgggtggcaatt atatcatatgatggaggtattaag tactatgcagactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctacaaatgaacaacctgagacttgaggacacggctgtgtattactgt gcgagaaaaggccccagaagagatggctccctacagcaatgcgatatggacgtc unmatched 15 213 10 5 6 9 4 6 1 2 4 8 JY8QFUQ01DPT8R 12376 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 1003 96.3 208/216 nt 95.83 207/216 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD2-8*01 F 2 8 7 8 8.7.8 [1.17.38.11] CARDNYGDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 63 of V-REGION: 1 nucleotides (from position 95 in the user submitted sequence), (cause frameshift) ctctggattcaccttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgtgttaatggtgatggggtagcacagcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgtgcaagagataattacggtgactactggggccagggaaccctggtcaccgtctcctcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaacgatgggaacgtggtcgtcgcatgcctggtccagggcttcttccccca NA unmatched, IGA2 57 84 [271, 273] a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| a166>g,I56>V(+ - +)|a172>g,S58>G(+ + -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|g258>t|g261>c|g267>c|g303>a| 320 224 216 8 4 4 3 1 0 0 0 0 0 0 3 0 1 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 51 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 23 21 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 117 114 108 6 4 2 1 1 0 0 0 0 0 0 3 0 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac ARDNYGDY IGHV3-74 IGHD2-8 IGHJ4 ggattcaccttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtctcacgt gttaatggtgatggggtagcaca gcctacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacactctctatctccaaatgaacagtctgagagccgaggacacggctgtatattactgt gcaagagataattacggtgactac unmatched 8 212 4 4 1 5 3 3 4 0 2 2 JY8QFUQ01DUD3U 2023 productive Homsap IGHV3-23*01 F, or Homsap IGHV3-23*04 F or Homsap IGHV3-23D*01 F or Homsap IGHV3-23D*02 F 819 86.64 188/217 nt NA Homsap IGHJ4*02 F (see comment) 69 60.42 29/48 nt Homsap IGHD6-19*01 F 1 8 8 10 8.8.10 [1.17.38.11] CMNELGYCSGGG in-frame + Other possibilities: Homsap_IGHJ4*03 (shorter alignment but highest percentage of identity) ctctggattcacttttaggagtcatatgatgagttgggtccgccagactccagggaaggggctggaatgggtctcaagtattcgagccagtggtgataggacacactatgcagactccgtgaggggccgcttcaccatctccagagacaactccaagaacacgatgtatttgcaaatgcacagcctgagagtcgacgacacggccgtatactactgtatgaacgaactcggatattgtagtggtgggggggcccagggagccctggtctccgtctgcctcagcatccccgaccagccccaaggtcttcccgctgaccctcgncagcaccccccaagatgggaacgtggtcgtcgcatgccctggtccaggggcttcttccccca NA unmatched, IGA2 64 79 [279] c87>t|c105>g,S35>R(- - -)|c108>t|t109>c,Y37>H(+ - -)|g112>a,A38>M(+ - -)|c113>t,A38>M(+ - -)|c114>g,A38>M(+ - -)|c120>t|g133>a,A45>T(- - -)|g153>a|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)|a169>c,S57>R(- - -)|t171>a,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g188>a,G63>D(- - -)|c192>g,S64>R(- - -)|t196>c,Y66>H(+ - -)|c201>t|a215>g,K72>R(+ + +)|g225>c|t246>c|c259>a,L87>M(+ + -)|c265>t|a274>c,N92>H(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t306>c|g313>a,A105>M(+ - -)|c314>t,A105>M(+ - -)| c87>t|c105>g,S35>R(- - -)|c108>t|t109>c,Y37>H(+ - -)|g112>a,A38>M(+ - -)|c113>t,A38>M(+ - -)|c114>g,A38>M(+ - -)| c120>t|g133>a,A45>T(- - -)|g153>a|g163>a,A55>S(- + -)|c164>g,A55>S(- + -)| a169>c,S57>R(- - -)|t171>a,S57>R(- - -)|g173>c,G58>A(- + -)|t174>c,G58>A(- + -)|g188>a,G63>D(- - -)|c192>g,S64>R(- - -)| t196>c,Y66>H(+ - -)|c201>t|a215>g,K72>R(+ + +)|g225>c|t246>c|c259>a,L87>M(+ + -)|c265>t|a274>c,N92>H(- - -)|c287>t,A96>V(+ - +)|g291>c,E97>D(+ - +)|t306>c| g313>a,A105>M(+ - -)|c314>t,A105>M(+ - -)|a318>c,K106>N(+ - -)| 320 225 193 32 9 23 1 6 8 5 3 1 0 1 3 4 0 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 2 5 0 1 3 1 0 0 0 0 0 2 0 0 51 51 46 5 2 3 0 3 1 0 0 0 0 0 0 1 0 0 30 24 18 6 0 6 0 1 0 1 1 0 0 1 1 1 0 0 117 114 103 11 5 6 1 0 3 3 1 1 0 0 2 0 0 0 8 8 5 3 0 3 0 1 1 0 1 0 0 0 0 0 0 0 ta,38-39(FR1)|ta,109-110(CDR1)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,316-317(CDR3)| tt,13-14(FR1)|tt,34-35(FR1)|ta,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,174-175(CDR2)|ta,189-190(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|tt,246-247(FR3)|ta,262-263(FR3)|ta,302-303(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|ggta,36-39(FR1)|agca,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agct,162-165(FR2)|ggta,172-175(CDR2)|ggta,187-190(CDR2)|ggtt,224-227(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|tgcc,111-114(CDR1)|agct,118-121(FR2)|agct,162-165(FR2)|tact,196-199(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacttttaggagtcatatg atgagttgggtccgccagactccagggaaggggctggaatgggtctcaagt attcgagccagtggtgataggaca cactatgcagactccgtgaggggccgcttcaccatctccagagacaactccaagaacacgatgtatttgcaaatgcacagcctgagagtcgacgacacggccgtatactactgt atgaacgaactcggatattgtagtggtggg MNELGYCSGG IGHV3-23 IGHD6-19 IGHJ4 ggattcacttttaggagtcatatg atgagttgggtccgccagactccagggaaggggctggaatgggtctcaagt attcgagccagtggtgataggaca cactatgcagactccgtgaggggccgcttcaccatctccagagacaactccaagaacacgatgtatttgcaaatgcacagcctgagagtcgacgacacggccgtatactactgt atgaacgaactcggatattgtagtggtggg unmatched 29 213 18 11 12 20 6 9 7 2 9 11 JY8QFUQ01DV4HU 6768 productive (see comment) Homsap IGHV3-7*01 F 882 89.4 194/217 nt 88.94 193/217 nt Homsap IGHJ1*01 F (see comment) 161 78.85 41/52 nt Homsap IGHD6-19*01 F 1 8 8 10 8.8.10 [1.17.38.11] CVVSSGWLFHYW in-frame + Potentially, because of detected insertion/deletion Other possibilities: Homsap_IGHJ4*02 (shorter alignment but highest percentage of identity) in FR3-IMGT at codon 92 of V-REGION: 'N' (1 nucleotides at position 179 to 179 in the user submitted sequence), (cause frameshift); ctctggattcacctctcctagatactggatgaattgggtccgccaggcttccgggaaggggctggagtgggtggccaacataaagcaagacggaagtgaggaaaactttgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaattcaatgtctctacaaatgNaacagcctgagagtcgaggacacggctgtatattattgcgttgttagcagtggctggttgttccactactggggccagggctcccgggtcaccgtctcctcagcctccaccaagggccagtcggtcttccccctgcgccctnctccaggagcacctccgagagcacagcggccctggctgccctggtcaaggatacttccccgaaccggtgacggtgtcgtggaactca NA unmatched, IGG2 71 62 [279, 280] t89>c,F30>S(- - -)|a103>c,S35>P(+ - -)|g104>c,S35>P(+ - -)|c108>a,S36>R(- - -)|t111>c|g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|c136>t,P46>S(+ - -)|a138>c,P46>S(+ - -)|t177>c|a193>g,K65>E(+ - -)|t196>a,Y66>N(- - -)|a200>t,Y67>F(- + -)|a225>g|a245>g,N82>S(- - -)|c255>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|c287>t,A96>V(+ - +)|g303>a|c309>t| t89>c,F30>S(- - -)|a103>c,S35>P(+ - -)|g104>c,S35>P(+ - -)|c108>a,S36>R(- - -)|t111>c| g119>a,S40>N(- - -)|c120>t,S40>N(- - -)|c136>t,P46>S(+ - -)|a138>c,P46>S(+ - -)| t177>c|a193>g,K65>E(+ - -)| t196>a,Y66>N(- - -)|a200>t,Y67>F(- + -)|a225>g|a245>g,N82>S(- - -)|c255>t|c259>a,L87>M(+ + -)|a263>c,Y88>S(+ - -)|g267>a|c287>t,A96>V(+ - +)|g303>a|c309>t|t312>c| c314>t,A105>V(+ - +)|g315>t,A105>V(+ - +)|a316>g,R106>V(- - -)|g317>t,R106>V(- - -)|a318>t,R106>V(- - -)|g319>a|a320>g| 311 (320) 216 (225) 194 (195) 22 (30) 7 (10) 15 (20) 3 (5) 3 (4) 5 (6) 3 (4) 3 2 1 (2) 1 1 0 0 (2) 0 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 19 5 1 4 0 0 0 2 1 1 0 0 1 0 0 0 51 51 47 4 0 4 0 1 2 0 1 0 0 0 0 0 0 0 30 24 22 2 1 1 1 0 0 1 0 0 0 0 0 0 0 0 116 (117) 114 102 11 5 6 2 2 3 0 1 1 1 1 0 0 0 0 0 (8) 0 0 (1) 0 (7) 0 (2) 0 (5) 0 (2) 0 (1) 0 (1) 0 (0) 0 0 0 (1) 0 0 0 0 (2) 0 ta,105-106(CDR1)|ta,109-110(CDR1)|aa,142-143(FR2)|aa,163-164(FR2)|ta,167-168(CDR2)|aa,169-170(CDR2)|aa,173-174(CDR2)|aa,186-187(CDR2)|aa,193-194(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,34-35(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|tt,111-112(CDR1)|ta,167-168(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agta,103-106(CDR1)|agct,118-121(FR2)|ggct,132-135(FR2)|ggct,146-149(FR2)|agca,170-173(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcacctctcctagatactgg atgaattgggtccgccaggcttccgggaaggggctggagtgggtggccaac ataaagcaagacggaagtgaggaa aactttgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaattcaatgtctctacaaatgaacagcctgagagtcgaggacacggctgtatattattgc gttgttagcagtggctggttgttccactac VVSSGWLFHY IGHV3-7 IGHD6-19 IGHJ1 ggattcacctctcctagatactgg atgaattgggtccgccaggcttccgggaaggggctggagtgggtggccaac ataaagcaagacggaagtgaggaa aactttgtggactctgtgaagggccggttcaccatctccagagacagcgccaagaattcaatgtctctacaaatgaacagcctgagagtcgaggacacggctgtatattattgc gttgttagcagtggctggttgttccactac unmatched 22 213 14 8 8 11 6 11 5 2 10 5 JY8QFUQ01DV8LF 10599 productive Homsap IGHV3-74*03 F 1031 97.25 212/218 nt NA Homsap IGHJ3*02 F 205 90 45/50 nt Homsap IGHD3-16*01 F 2 8 8 10 8.8.10 [1.17.38.11] CATGPDYAYAMW in-frame + cctctggattcaccttcagtcgctactggatgcactgggtccgccaagctccagggaagggcctggtgtgggtctcacgtattaaaagtgatgggattagcacaacgtacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacggtgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtactactgtgcaaccgggcccgattatgcgtatgctatgtggggccaagggacaatggtcaccgtctcttcagcatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggctccagggcttcttccccca NA unmatched, IGA2 57 89 [279, 280] a106>c,S36>R(- - -)|g147>c|t171>a,N57>K(+ - -)|g188>t,S63>I(- - -)|c259>g,L87>V(+ - +)|t306>c| a106>c,S36>R(- - -)| g147>c| t171>a,N57>K(+ - -)|g188>t,S63>I(- - -)| c259>g,L87>V(+ - +)|t306>c| g317>c,R106>T(- - -)|a318>c,R106>T(- - -)|a320>g| 316 (320) 222 (226) 216 (217) 6 (9) 2 (3) 4 (6) 0 (1) 0 0 1 1 (2) 0 0 1 1 (2) 1 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 30 24 22 2 0 2 0 0 0 0 0 0 0 1 0 0 1 0 117 114 112 2 1 1 0 0 0 1 0 0 0 0 0 1 0 0 4 (8) 4 4 (5) 0 (3) 0 (1) 0 (2) 0 (1) 0 0 0 0 (1) 0 0 0 0 (1) 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcaccttcagtcgctactgg atgcactgggtccgccaagctccagggaagggcctggtgtgggtctcacgt attaaaagtgatgggattagcaca acgtacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacggtgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtactactgt gcaaccgggcccgattatgcgtatgctatg ATGPDYAYAM IGHV3-74 IGHD3-16 IGHJ3 ggattcaccttcagtcgctactgg atgcactgggtccgccaagctccagggaagggcctggtgtgggtctcacgt attaaaagtgatgggattagcaca acgtacgcggactccgtgaagggccgattcaccatctccagagacaacgccaagaacacggtgtatctgcaaatgaacagtctgagagccgaggacacggctgtgtactactgt gcaaccgggcccgattatgcgtatgctatg unmatched 6 213 1 5 0 3 1 3 2 0 1 3 JY8QFUQ01DVBU0 5305 productive (see comment) Homsap IGHV3-74*01 F, or Homsap IGHV3-74*02 F or Homsap IGHV3-74*03 F 940 93.06 201/216 nt 92.59 200/216 nt Homsap IGHJ4*02 F 213 93.75 45/48 nt Homsap IGHD6-19*01 F 3 8 7 12 8.7.12 [1.17.38.11] CARDTGQWKDFDYW in-frame + Potentially, because of detected insertion/deletion in CDR2-IMGT, from codon 58 of V-REGION: 1 nucleotides (from position 87 in the user submitted sequence), (cause frameshift) ctctggattcatcttcagtagctactggatgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgtagtaatacggggggactgacacagcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgtgcaagagatacggggcagtggaaggactttgactactggggccagggaaccctggtcaccgtctccttagcatccccgaccagccccaaaggttcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttccc NA unmatched, IGA2 57 74 [286, 285, 283] c86>t,T29>I(- - -)|c159>g|t167>g,I56>S(- - -)|t174>c|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)|a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| c86>t,T29>I(- - -)| c159>g| t167>g,I56>S(- - -)|t174>c|a176>g,D59>G(- - -)|t177>g,D59>G(- - -)|g188>c,S63>T(+ - +)|a190>g,S64>D(- - -)|g191>a,S64>D(- - -)| a196>g,S66>A(- + -)|g197>c,S66>A(- + -)|a238>c|c248>g,A83>G(- + -)|c249>g,A83>G(- + -)|g303>t| 320 224 209 15 4 11 3 1 1 1 1 0 0 0 2 3 1 2 78 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 23 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 51 51 50 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 30 23 16 7 1 6 2 1 0 1 0 0 0 0 1 0 0 2 117 114 108 6 2 4 1 0 0 0 1 0 0 0 1 2 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ta,35-36(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|aa,131-132(FR2)|aa,142-143(FR2)|ta,165-166(FR2-CDR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|aa,195-196(CDR2)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)|aa,315-316(CDR3)| tt,34-35(FR1)|tt,38-39(FR1)|tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,165-166(FR2-CDR2)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,189-190(CDR2)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|ggct,31-34(FR1)|agtt,36-39(FR1)|agta,103-106(CDR1)|agct,132-135(FR2)|ggct,146-149(FR2)|agta,187-190(CDR2)|agct,196-199(FR3)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|agct,132-135(FR2)|agct,196-199(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| ctct ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgt agtaatacggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac ARDTGQWKDFDY IGHV3-74 IGHD6-19 IGHJ4 ggattcatcttcagtagctactgg atgcactgggtccgccaagctccagggaaggggctggtgtgggtgtcacgt agtaatacggggggactgacaca gcctacgcggactccgtgaagggccgattcaccatctcccgagacaacgggaagaacacgctgtatctgcaaatgaacagtctgagagccgaggacacggctgtttattactgt gcaagagatacggggcagtggaaggactttgactac unmatched 15 212 6 9 2 8 4 7 3 1 4 7 JY8QFUQ01DXDOM 21713 productive (see comment) Homsap IGHV3-21*01 F, or Homsap IGHV3-21*02 F or Homsap IGHV3-21*03 F (see comment) 746 83.26 179/215 nt 82.79 178/215 nt Homsap IGHJ6*02 F 179 77.05 47/61 nt Homsap IGHD2-15*01 F 3 8 7 12 8.7.12 [1.17.38.11] CARDKVISAGRDVW in-frame + Potentially, because of detected insertion/deletion The submitted sequence and the closest germline V-GENE allele show different CDR2-IMGT amino acid lengths (7 AA in JY8QFUQ01DXDOM ; 8 AA in Homsap_IGHV3-21*01), and low V-REGION identity (83,26% ) this may indicate potential nucleotide insertion(s) and/or deletion(s) which are not dealt in this release in FR3-IMGT at codon 81 of V-REGION: 'G' (1 nucleotides at position 144 to 144 in the user submitted sequence), (cause frameshift); cctctggattcagtttcactggttttaccgtgatctgggtccgccaggctccaaggaaggggctggaatggatctcatccgtcactactaatggtctcacgtactacgcagactcagtagagggccgattcaacatctccaggGgacaacgccaacaatttagtgtttctgcaaatgaacagcctgagagtcgaggacactggtgtatattattgtgcgagagataaagtgatctctgccggacgggacgtctggggccaagggaccacggtcaccgtctcctcaacatccccgaccagccccaaggtcttcccgctgagcctcgacagcaccccccgaagatgggaacgtggtcgtcgcatgcctggtccagggcttcttcccccc NA unmatched, IGA2 57 89 [283, 284] c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>c,S35>T(+ - +)|a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)|a110>t,Y37>F(- + -)|g113>c,S38>T(+ - +)|a115>g,M39>V(+ - -)|a119>t,N40>I(- - -)|g139>a,G47>R(- - -)|g153>a|g157>a,V53>I(+ - +)|a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>c,S57>T(+ - +)|g173>c,S58>T(+ - +)|g176>a,S59>N(- - -)|a187>g,S63>G(+ + -)|t190>c,Y64>L(- - -)|a191>t,Y64>L(- - -)|t194>c,I65>T(- - -)|a195>g,I65>T(- - -)|g213>a|a214>g,K72>E(+ - -)|c230>a,T77>N(- + -)|a240>g|g252>c,K84>N(+ - -)|c255>t|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|g297>t|c299>g,A100>G(- + -)|g303>a|c309>t| c86>g,T29>S(+ - +)|c87>t,T29>S(+ - +)|g104>c,S35>T(+ - +)|a106>g,S36>G(+ + -)|c108>t,S36>G(+ + -)|a110>t,Y37>F(- + -)|g113>c,S38>T(+ - +)| a115>g,M39>V(+ - -)|a119>t,N40>I(- - -)|g139>a,G47>R(- - -)|g153>a|g157>a,V53>I(+ - +)| a166>g,I56>V(+ - +)|t168>c,I56>V(+ - +)|g170>c,S57>T(+ - +)|g173>c,S58>T(+ - +)|g176>a,S59>N(- - -)|a187>g,S63>G(+ + -)|t190>c,Y64>L(- - -)|a191>t,Y64>L(- - -)|t194>c,I65>T(- - -)|a195>g,I65>T(- - -)| g213>a|a214>g,K72>E(+ - -)|c230>a,T77>N(- + -)|a240>g|g252>c,K84>N(+ - -)|c255>t|c257>t,S86>L(- - -)|c259>g,L87>V(+ - +)|a263>t,Y88>F(- + -)|c287>t,A96>V(+ - +)|g297>t|c299>g,A100>G(- + -)|g303>a|c309>t| 320 223 187 36 7 29 7 6 6 3 0 1 4 0 5 3 1 0 78 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 24 17 7 0 7 1 0 2 0 0 0 1 0 2 1 0 0 51 51 46 5 1 4 1 3 0 0 0 0 1 0 0 0 0 0 30 21 11 10 0 10 3 1 0 3 0 0 1 0 2 0 0 0 117 114 100 14 6 8 2 2 4 0 0 1 1 0 1 2 1 0 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 aa,40-41(FR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|aa,118-119(FR2)|aa,142-143(FR2)|ta,168-169(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|ta,190-191(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|aa,214-215(FR3)|aa,244-245(FR3)|aa,250-251(FR3)|aa,253-254(FR3)|ta,262-263(FR3)|aa,269-270(FR3)|aa,274-275(FR3)|ta,304-305(FR3)|ta,307-308(FR3)| tt,82-83(CDR1)|tt,88-89(CDR1)|ta,105-106(CDR1)|ta,109-110(CDR1)|ta,111-112(CDR1)|tt,167-168(CDR2)|ta,171-172(CDR2)|ta,174-175(CDR2)|ta,186-187(CDR2)|tt,189-190(CDR2)|ta,194-195(CDR2)|ta,196-197(FR3)|ta,199-200(FR3)|tt,226-227(FR3)|ta,262-263(FR3)|ta,304-305(FR3)|tt,306-307(FR3)| agct,8-11(FR1)|agta,103-106(CDR1)|agca,112-115(CDR1)|ggct,132-135(FR2)|ggct,146-149(FR2)|agta,169-172(CDR2)|agta,184-187(CDR2)|ggct,297-300(FR3)| agct,8-11(FR1)|agcc,41-44(FR1)|agcc,72-75(FR1)|agct,106-109(CDR1)|aact,118-121(FR2)|tact,196-199(FR3)|aact,253-256(FR3)|agcc,277-280(FR3)|agcc,285-288(FR3)|tact,307-310(FR3)| cctct ggattcagtttcactggttttacc gtgatctgggtccgccaggctccaaggaaggggctggaatggatctcatcc gtcactactaatggtctcacg tactacgcagactcagtagagggccgattcaacatctccagggacaacgccaacaatttagtgtttctgcaaatgaacagcctgagagtcgaggacactggtgtatattattgt gcgagagataaagtgatctctgccggacgggacgtc ARDKVISAGRDV IGHV3-21 IGHD2-15 IGHJ6 ggattcagtttcactggttttacc gtgatctgggtccgccaggctccaaggaaggggctggaatggatctcatcc gtcactactaatggtctcacg tactacgcagactcagtagagggccgattcaacatctccagggacaacgccaacaatttagtgtttctgcaaatgaacagcctgagagtcgaggacactggtgtatattattgt gcgagagataaagtgatctctgccggacgggacgtc unmatched 36 210 22 14 12 22 10 14 7 0 12 17