Mercurial > repos > dereeper > sniplay
diff MDSplot/mdsplot.xml @ 3:345f88a8f483 draft
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author | dereeper |
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date | Fri, 10 Jul 2015 10:38:43 -0400 |
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children | 10627af23f10 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/MDSplot/mdsplot.xml Fri Jul 10 10:38:43 2015 -0400 @@ -0,0 +1,232 @@ +<tool id="sniplay_mdsplot" name="MDS plot" version="1.1.1"> + + <!-- [REQUIRED] Tool description displayed after the tool name --> + <description> IBS matrix / multi-dimensional scaling</description> + + <!-- [OPTIONAL] 3rd party tools, binaries, modules... required for the tool to work --> + <requirements> + <requirement type="binary">perl</requirement> + <requirement type="package" version="1.07">plink</requirement> + </requirements> + + <!-- [OPTIONAL] Command to be executed to get the tool's version string --> + <version_command> +<!-- + tool_binary -v +--> + </version_command> + + <!-- [REQUIRED] The command to execute --> + <command interpreter="bash"> + mdsplot.sh $fileped $filemap $fileout_label $fileout_matrix $fileout_plot $fileout_log + </command> + + <!-- [REQUIRED] Input files and tool parameters --> + <inputs> + <param name="fileped" type="data" format="txt" optional="false" label="PED input" /> + <param name="filemap" type="data" format="txt" optional="false" label="MAP input" help="4 columns tabular file: chromosome, snp id, genetic distance, bp position"/> + <param name="fileout_label" type="text" value="analyse" label="Output name" help="Output name for tabular files" /> + </inputs> + + <!-- [REQUIRED] Output files --> + <outputs> + <data name="fileout_matrix" type="data" format="tabular" label="${fileout_label}.ibs_matrix.txt" /> + <data name="fileout_plot" type="data" format="tabular" label="${fileout_label}.mds_plot.txt" /> + <data name="fileout_log" type="data" format="txt" label="${fileout_label}.log" /> + </outputs> + + <!-- [STRONGLY RECOMMANDED] Exit code rules --> + <stdio> + <!-- [HELP] If no exit code rule is defined, the tool will stop if anything is written to STDERR --> + <exit_code range="1:" level="fatal" /> + </stdio> + + <!-- [OPTIONAL] Tests to be run manually by the Galaxy admin --> + <tests> + <!-- [HELP] Test files have to be in the ~/test-data directory --> + + <test> + <param name="fileped" value="input.ped" /> + <param name="filemap" value="input.map" /> + <output name="fileout_matrix" file="output.ibs_matrix.txt" /> + <output name="fileout_plot" file="output.mds_plot.txt" /> + <output name="fileout_log" file="output.log" /> + </test> + + <!-- [HELP] Multiple tests can be defined with different parameters --> +<!-- + <test> + </test> +--> + </tests> + + <!-- [OPTIONAL] Help displayed in Galaxy --> + <help> + + +.. class:: infomark + +**Authors** plink_ + +.. _plink: http://pngu.mgh.harvard.edu/purcell/plink/ + + | "PLINK: a toolset for whole-genome association and population-based linkage analysis.", **Purcell S, Neale B, Todd-Brown K, Thomas L, Ferreira MAR, Bender D, Maller J, Sklar P, de Bakker PIW, Daly MJ, Sham PC.**, American Journal of Human Genetics, 81, 2007. + + +.. class:: infomark + +**Galaxy integration** Andres Gwendoline, Institut Français de Bioinformatique. + +.. class:: infomark + +**Support** For any questions about Galaxy integration, please send an e-mail to support.abims@sb-roscoff.fr + +.. class:: infomark + +**Please cite** "SNiPlay3: a web-based application for exploration and large scale analyses of genomic variations", **Dereeper A. et al.**, Nucl. Acids Res. (1 july 2015) 43 (W1). + +--------------------------------------------------- + + + +======== +MDS plot +======== + +----------- +Description +----------- + + Compute an IBS matrix and a multi-dimensional scaling. + + +----------------- +Workflow position +----------------- + +**Upstream tool** + +=============== ========================== =============== +Name output file(s) format +=============== ========================== =============== +VCFtools Filter PED and MAP file tabular and MAP +=============== ========================== =============== + + + +---------- +Input file +---------- + +PED file + +MAP file + 4 columns tabular file: chromosome, snp id, genetic distance, bp position + + +---------- +Parameters +---------- + +Output name + Output base name for the ouput files + + +------------ +Output files +------------ + +Output_name.ibs_matrix.txt + Tabular file with IBS matrix + +Output_name.mds_plot.txt + File to construct mds plot + +Output_name.log + Log file + +------------ +Dependencies +------------ +plink + version 1.07 + +--------------------------------------------------- + +--------------- +Working example +--------------- + +Input files +=========== + +PED file +----------- + +:: + + IRAT112 1 0 0 1 1 1 1 4 4 ... + IAC25 1 0 0 1 1 1 1 4 4 ... + CIRAD409 1 0 0 1 1 3 3 1 1 ... + + +MAP file +----------- + +:: + + Chr1 Chr1:4299 0 4299 + Chr1 Chr1:26710 0 26710 + Chr1 Chr1:56184 0 56184 + Chr1 Chr1:93272 0 93272 + + + +Parameters +========== + +Output name -> densities + + +Output files +============ + +densities.ibs_matrix.txt +------------------------ + +:: + + Individuals IRAT112 IAC25 IAC165 KARASUKARASURANKASU DOURADOPRECOCE ... + IRAT112 1 0.93691 0.937407 0.734724 0.943368 ... + IAC25 0.93691 1 0.958768 0.723299 0.965723 ... + + +densities.mds_plot.txt +---------------------- + +:: + + IRAT112 -0.0969382 0.0376036 + IAC25 -0.0918126 0.0501177 + + + + </help> + <citations> + <!-- [HELP] As DOI or BibTex entry --> + <citation type="bibtex">@article{Dereeper03062015, +author = {Dereeper, Alexis and Homa, Felix and Andres, Gwendoline and Sempere, Guilhem and Sarah, Gautier and Hueber, Yann and Dufayard, Jean-François and Ruiz, Manuel}, +title = {SNiPlay3: a web-based application for exploration and large scale analyses of genomic variations}, +year = {2015}, +doi = {10.1093/nar/gkv351}, +abstract ={SNiPlay is a web-based tool for detection, management and analysis of genetic variants including both single nucleotide polymorphisms (SNPs) and InDels. Version 3 now extends functionalities in order to easily manage and exploit SNPs derived from next generation sequencing technologies, such as GBS (genotyping by sequencing), WGRS (whole gre-sequencing) and RNA-Seq technologies. Based on the standard VCF (variant call format) format, the application offers an intuitive interface for filtering and comparing polymorphisms using user-defined sets of individuals and then establishing a reliable genotyping data matrix for further analyses. Namely, in addition to the various scaled-up analyses allowed by the application (genomic annotation of SNP, diversity analysis, haplotype reconstruction and network, linkage disequilibrium), SNiPlay3 proposes new modules for GWAS (genome-wide association studies), population stratification, distance tree analysis and visualization of SNP density. Additionally, we developed a suite of Galaxy wrappers for each step of the SNiPlay3 process, so that the complete pipeline can also be deployed on a Galaxy instance using the Galaxy ToolShed procedure and then be computed as a Galaxy workflow. SNiPlay is accessible at http://sniplay.southgreen.fr.}, +URL = {http://nar.oxfordjournals.org/content/early/2015/06/03/nar.gkv351.abstract}, +eprint = {http://nar.oxfordjournals.org/content/early/2015/06/03/nar.gkv351.full.pdf+html}, +journal = {Nucleic Acids Research} +} + + </citation> + + </citations> + +</tool>