Mercurial > repos > dereeper > sniplay
diff check_gwas_inputs/CheckGWASInputs.xml @ 3:345f88a8f483 draft
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author | dereeper |
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date | Fri, 10 Jul 2015 10:38:43 -0400 |
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children | 10627af23f10 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/check_gwas_inputs/CheckGWASInputs.xml Fri Jul 10 10:38:43 2015 -0400 @@ -0,0 +1,184 @@ +<tool id="check_GWAS_inputs" name="Check GWAS Inputs" version="1.1"> + <description>checks concordance between input files for GWAS analysis</description> + <command interpreter="bash">./CheckGWASInputs.sh $hapmap $trait $out_hapmap $out_trait $stats + </command> + <inputs> + <param format="txt" name="hapmap" type="data" label="Allelic file in Hapmap format" help="Allelic file in Hapmap format"/> + <param format="txt" name="trait" type="data" label="Trait file" help="Phenotypic file"/> + </inputs> + <outputs> + <data format="txt" name="out_hapmap" label="Hapmap output"/> + <data format="txt" name="out_trait" label="Trait output"/> + <data format="txt" name="stats" label="Logfile and statistics"/> + </outputs> + <!-- [STRONGLY RECOMMANDED] Exit code rules --> + <stdio> + <!-- [HELP] If no exit code rule is defined, the tool will stop if anything is written to STDERR --> + <exit_code range="1:" level="fatal" /> + </stdio> + + <!-- [OPTIONAL] Tests to be run manually by the Galaxy admin --> + <tests> + <!-- [HELP] Test files have to be in the ~/test-data directory --> + <!-- [HELP] Multiple tests can be defined with different parameters --> +<!-- + <test> + </test> +--> + </tests> + <help> + + <![CDATA[ + + +.. class:: infomark + +**Authors** South Green + + | **Please cite** "SNiPlay3: a web-based application for exploration and large scale analyses of genomic variations", **Dereeper A. et al.**, Nucl. Acids Res. (1 july 2015) 43 (W1). + +.. class:: infomark + +**Galaxy integration** South Green. + +--------------------------------------------------- + + +=============== +CheckGWASInputs +=============== + +----------- +Description +----------- + + | CheckGWASInputs checks concordance between input files for GWAS analysis. + + +----------------- +Workflow position +----------------- + +**Upstream tool** + +=============== ====================== =========== +Name output file(s) format +=============== ====================== =========== +VCF to Hapmap Hapmap file hapmap +=============== ====================== =========== + + + + +---------- +Input file +---------- + +Hapmap file + Allelic file in Hapmap format + +Trait file + Phenotypic file + +------------ +Output files +------------ + +Hapmap output + +Trait output + +Logfile and statistics + +--------------------------------------------------- + +--------------- +Working example +--------------- + +Input files +=========== + +Hapmap file +----------- + +:: + + rs# alleles chrom pos strand assembly# center protLSID assayLSID panel QCcode Ind1 Ind2 + SNP1 A/T 1 3102 + assembly NA NA NA speciesname NA AA AA AA + SNP2 A/T 1 4648 + assembly NA NA NA speciesname NA AA AA AA + +Trait file +---------- + +:: + + <Trait> Test + Ind1 -2.9985936006411 + Ind2 -2.68669426456267 + +Output files +============ + +Hapmap output +------------- + +:: + + rs# alleles chrom pos strand assembly# center protLSID assayLSID panel QCcode Ind1 Ind2 Ind3 Ind4 + SNP1 A/T 1 3102 + assembly NA NA NA speciesname NA AA AA AA AA AA + SNP2 A/T 1 4648 + assembly NA NA NA speciesname NA AA AA AA AA TT + + +Trait output +------------ + +:: + + <Trait> Test + Ind429 -26.2142525264157 + Ind373 12.0306115988504 + Ind81 1.98118654229534 + +Logfile and statistics +---------------------- + +:: + + + ============================================== + Individuals + ============================================== + Individuals in hapmap file: 500 + Individuals in trait file: 500 + Individuals found in both files: 500 + ============================================== + Markers + ============================================== + Discarded markers: + Monomorphic: 0 + Not biallelic: 0 + Modified markers: + Difference in variation: 0 + + ]]> + + + </help> + <citations> + <!-- [HELP] As DOI or BibTex entry --> + <citation type="bibtex">@article{Dereeper03062015, +author = {Dereeper, Alexis and Homa, Felix and Andres, Gwendoline and Sempere, Guilhem and Sarah, Gautier and Hueber, Yann and Dufayard, Jean-François and Ruiz, Manuel}, +title = {SNiPlay3: a web-based application for exploration and large scale analyses of genomic variations}, +year = {2015}, +doi = {10.1093/nar/gkv351}, +abstract ={SNiPlay is a web-based tool for detection, management and analysis of genetic variants including both single nucleotide polymorphisms (SNPs) and InDels. Version 3 now extends functionalities in order to easily manage and exploit SNPs derived from next generation sequencing technologies, such as GBS (genotyping by sequencing), WGRS (whole gre-sequencing) and RNA-Seq technologies. Based on the standard VCF (variant call format) format, the application offers an intuitive interface for filtering and comparing polymorphisms using user-defined sets of individuals and then establishing a reliable genotyping data matrix for further analyses. Namely, in addition to the various scaled-up analyses allowed by the application (genomic annotation of SNP, diversity analysis, haplotype reconstruction and network, linkage disequilibrium), SNiPlay3 proposes new modules for GWAS (genome-wide association studies), population stratification, distance tree analysis and visualization of SNP density. Additionally, we developed a suite of Galaxy wrappers for each step of the SNiPlay3 process, so that the complete pipeline can also be deployed on a Galaxy instance using the Galaxy ToolShed procedure and then be computed as a Galaxy workflow. SNiPlay is accessible at http://sniplay.southgreen.fr.}, +URL = {http://nar.oxfordjournals.org/content/early/2015/06/03/nar.gkv351.abstract}, +eprint = {http://nar.oxfordjournals.org/content/early/2015/06/03/nar.gkv351.full.pdf+html}, +journal = {Nucleic Acids Research} +} + + </citation> + + </citations> +</tool>