Mercurial > repos > dereeper > sniplay
diff GetHaplotypesFromPhasedVCF/getHaplotypesFromPhasedVCF.xml @ 6:ebb0ac9b6fa9 draft
planemo upload
author | gandres |
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date | Mon, 23 May 2016 17:49:17 -0400 |
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children | c6640c49fd01 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/GetHaplotypesFromPhasedVCF/getHaplotypesFromPhasedVCF.xml Mon May 23 17:49:17 2016 -0400 @@ -0,0 +1,171 @@ +<tool id="getHaplotypesFromPhasedVCF" name="Get Haplotypes From Phased VCF" version="1.0.0"> + <requirements> + <requirement type="binary">perl</requirement> + </requirements> + <stdio> + <exit_code range="1:" /> + </stdio> + <command interpreter="perl"> + GetHaplotypesFromPhasedVCF.pl $input $output_label && mv ${output_label}.distinct_haplotypes.txt $output_distinct && mv ${output_label}.haplo.fas $output_haplo + </command> + <inputs> + <param type="data" name="input" format="vcf" label="Phased VCF" /> + <param type="text" name="output_label" label="Output_label" value='Haplotypes' /> + </inputs> + <outputs> + <data name="output_distinct" format="txt" label="${output_label}.distinct_haplotypes.txt"/> + <data name="output_haplo" format="fasta" label="${output_label}.haplo.fas"/> + </outputs> + <tests> + <test> + <param name="input" value="getHaplotypesFromPhasedVCF-input.vcf"/> + <output name="output_distinct" file="getHaplotypesFromPhasedVCF-result.distinct_haplotypes.txt"/> + <output name="output_haplo" file="getHaplotypesFromPhasedVCF-result.haplo.fas"/> + </test> + </tests> + <help><![CDATA[ + +.. class:: infomark + +**Authors** Dereeper Alexis (alexis.dereeper@ird.fr), IRD, South Green platform + + | **Please cite** "SNiPlay3: a web-based application for exploration and large scale analyses of genomic variations", **Dereeper A. et al.**, Nucl. Acids Res. (1 july 2015) 43 (W1). + +.. class:: infomark + +**Galaxy integration** Andres Gwendoline, Institut Français de Bioinformatique. + +.. class:: infomark + +**Support** For any questions, please send an e-mail to support.abims@sb-roscoff.fr + +--------------------------------------------------- + +============================== +Get Haplotypes From Phased VCF +============================== + +----------- +Description +----------- + + | Get Haplotype from phased VCF + +----------------- +Workflow position +----------------- + +**Upstream tool** + +=============== ========================== ======= +Name output file(s) format +=============== ========================== ======= +Beagle Phased VCF file VCF +=============== ========================== ======= + + +**Downstream tool** + +=============== ========================== =========== +Name input file(s) format +=============== ========================== =========== +=============== ========================== =========== + + +---------- +Input file +---------- + +VCF file + Phased VCF file + +---------- +Parameters +---------- + +Output file basename + Prefix for the output VCF file + +------------ +Output files +------------ + + +Text file + File describing haplotypes + +Fasta file + Fasta file with haplotypes + +--------------------------------------------------- + +--------------- +Working example +--------------- + +Input files +=========== + +VCF file +--------- + +:: + + #fileformat=VCFv4.1 + #FILTER=<ID=LowQual,Description="Low quality"> + #FORMAT=<ID=AD,Number=.,Type=Integer,Description="Allelic depths for the ref and alt alleles in the order listed"> + [...] + CHROM POS ID REF ALT QUAL FILTER INFO FORMAT AZUCENA + Chr1 4299 . G A . PASS AR2=1;DR2=1;AF=0.168 GT:DS:GP 0|0:0:1,0,0 + + +Parameters +========== + +Output name -> haplotypes + + +Output files +============ + +haplotypes.distinct_haplotypes.txt +---------------------------------- + +:: + + ===Chr10=== + haplo1:2:CIRAD403_1,CIRAD403_2, + TTTAAGAAATTCCTATATAGGTCTTCTAAGCGTATCTATTAACAT + haplo2:2:MAHAE_1,MAHAE_2, + TAAATCTTGGTGCTGATCTGATATTTAATGCGT + + +haplotypes.haplo.fas +-------------------- + +:: + + >Chr10_AZUCENA_1 + TTTAAGAAATTCCTATATAGGTCTTCTAAGCGTATCTATTAACAT + >Chr10_AZUCENA_2 + TAAATCTTGGTGCTGATCTGATATTTAATGCGT + + ]]></help> + <citations> + <!-- [HELP] As DOI or BibTex entry --> + <citation type="bibtex">@article{Dereeper03062015, + author = {Dereeper, Alexis and Homa, Felix and Andres, Gwendoline and Sempere, Guilhem and Sarah, Gautier and Hueber, Yann and Dufayard, Jean-François and Ruiz, Manuel}, + title = {SNiPlay3: a web-based application for exploration and large scale analyses of genomic variations}, + year = {2015}, + doi = {10.1093/nar/gkv351}, + abstract ={SNiPlay is a web-based tool for detection, management and analysis of genetic variants including both single nucleotide polymorphisms (SNPs) and InDels. Version 3 now extends functionalities in order to easily manage and exploit SNPs derived from next generation sequencing technologies, such as GBS (genotyping by sequencing), WGRS (whole gre-sequencing) and RNA-Seq technologies. Based on the standard VCF (variant call format) format, the application offers an intuitive interface for filtering and comparing polymorphisms using user-defined sets of individuals and then establishing a reliable genotyping data matrix for further analyses. Namely, in addition to the various scaled-up analyses allowed by the application (genomic annotation of SNP, diversity analysis, haplotype reconstruction and network, linkage disequilibrium), SNiPlay3 proposes new modules for GWAS (genome-wide association studies), population stratification, distance tree analysis and visualization of SNP density. Additionally, we developed a suite of Galaxy wrappers for each step of the SNiPlay3 process, so that the complete pipeline can also be deployed on a Galaxy instance using the Galaxy ToolShed procedure and then be computed as a Galaxy workflow. SNiPlay is accessible at http://sniplay.southgreen.fr.}, + URL = {http://nar.oxfordjournals.org/content/early/2015/06/03/nar.gkv351.abstract}, + eprint = {http://nar.oxfordjournals.org/content/early/2015/06/03/nar.gkv351.full.pdf+html}, + journal = {Nucleic Acids Research} + } + + }</citation> + + </citations> + +</tool>