Mercurial > repos > devteam > blat_mapping
diff blat_mapping.xml @ 0:807e3e50845a draft default tip
Imported from capsule None
author | devteam |
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date | Mon, 19 May 2014 12:33:35 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/blat_mapping.xml Mon May 19 12:33:35 2014 -0400 @@ -0,0 +1,42 @@ +<tool id="blat2wig" name="Coverage of the Reads" version="1.0.0"> + <description>in wiggle format</description> + <command interpreter="python">blat_mapping.py $input1 $output1</command> + <inputs> + <param name="input1" type="data" format="tabular" label="Alignment result"/> + </inputs> + <outputs> + <data name="output1" format="wig"/> + </outputs> + <tests> + <test> + <param name="input1" value="blat_mapping_test1.txt" ftype="tabular" /> + <output name="output1" file="blat_mapping_test1.out" /> + </test> + </tests> + <help> + +.. class:: warningmark + + To generate acceptable files, please use alignment program **BLAT** with option **-out=pslx**. + +.. class:: warningmark + + Please edit the database information by click on the pencil icon next to your dataset. Select the corresponding genome build. + +----- + +**What it does** + + This tool takes **BLAT pslx** output and returns a wig-like file showing the number of reads (coverage) mapped at each chromosome location. Use **Graph/Display Data --> Build custom track** tool to show the coverage mapping in UCSC Genome Browser. + +----- + +**Example** + + Showing reads coverage on human chromosome 22 (partial result) in UCSC Genome Browser Custom Track: + + .. image:: blat_mapping_example.png + :width: 600 + + </help> +</tool>