Mercurial > repos > devteam > blat_mapping
view blat_mapping.xml @ 0:807e3e50845a draft default tip
Imported from capsule None
author | devteam |
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date | Mon, 19 May 2014 12:33:35 -0400 |
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<tool id="blat2wig" name="Coverage of the Reads" version="1.0.0"> <description>in wiggle format</description> <command interpreter="python">blat_mapping.py $input1 $output1</command> <inputs> <param name="input1" type="data" format="tabular" label="Alignment result"/> </inputs> <outputs> <data name="output1" format="wig"/> </outputs> <tests> <test> <param name="input1" value="blat_mapping_test1.txt" ftype="tabular" /> <output name="output1" file="blat_mapping_test1.out" /> </test> </tests> <help> .. class:: warningmark To generate acceptable files, please use alignment program **BLAT** with option **-out=pslx**. .. class:: warningmark Please edit the database information by click on the pencil icon next to your dataset. Select the corresponding genome build. ----- **What it does** This tool takes **BLAT pslx** output and returns a wig-like file showing the number of reads (coverage) mapped at each chromosome location. Use **Graph/Display Data --> Build custom track** tool to show the coverage mapping in UCSC Genome Browser. ----- **Example** Showing reads coverage on human chromosome 22 (partial result) in UCSC Genome Browser Custom Track: .. image:: blat_mapping_example.png :width: 600 </help> </tool>