Mercurial > repos > devteam > bwa
annotate bwa.xml @ 26:2477830927ec draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 6e9628b1d92fdb358b79959ad54a456cfa46fa33
author | iuc |
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date | Fri, 17 May 2024 21:09:07 +0000 |
parents | e188dc7a68e6 |
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rev | line source |
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0 | 1 <?xml version="1.0"?> |
26
2477830927ec
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 6e9628b1d92fdb358b79959ad54a456cfa46fa33
iuc
parents:
25
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changeset
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2 <tool id="bwa" name="Map with BWA" version="@TOOL_VERSION@" profile="22.05"> |
18
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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changeset
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3 <description>- map short reads (< 100 bp) against reference genome</description> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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changeset
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4 <macros> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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5 <import>read_group_macros.xml</import> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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6 <import>bwa_macros.xml</import> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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7 <token name="@command_options@"> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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changeset
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8 #if str( $analysis_type.analysis_type_selector ) == "full": |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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9 -n ${analysis_type.n} |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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changeset
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10 -o ${analysis_type.o} |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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changeset
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11 -e ${analysis_type.e} |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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changeset
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12 -i ${analysis_type.i} |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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changeset
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13 -d ${analysis_type.d} |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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14 -l ${analysis_type.l} |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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15 -k ${analysis_type.k} |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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changeset
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16 -m ${analysis_type.m} |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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17 -M ${analysis_type.M} |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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18 -O ${analysis_type.O} |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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19 -E ${analysis_type.E} |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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20 -R ${analysis_type.R} |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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21 -q ${analysis_type.q} |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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22 #if str( $analysis_type.B ): |
0 | 23 -B ${analysis_type.B} |
18
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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24 #end if |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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25 #if str( $analysis_type.L ): |
4
ac30bfd3e2a8
planemo upload commit a50a3947aebc8a1d11bac39599f4efd8ed9a3bd5
devteam
parents:
3
diff
changeset
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26 -L ${analysis_type.L} |
2 | 27 #end if |
18
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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28 #end if |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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29 </token> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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30 <token name="@read_group_options@"> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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changeset
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31 #if $use_rg: |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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changeset
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32 @set_rg_string@ |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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33 -r '$rg_string' |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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34 #end if |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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35 </token> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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36 <xml name="advanced_pe_options"> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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37 <param name="adv_pe_options_selector" type="select" label="Set advanced paired end options?" |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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38 help="Provides additional controls"> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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changeset
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39 <option value="set">Set</option> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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40 <option value="do_not_set" selected="True">Do not set</option> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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41 </param> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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42 <when value="set"> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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changeset
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43 <param name="a" type="integer" value="500" |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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changeset
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44 label="Maximum insert size for a read pair to be considered being mapped properly." |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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changeset
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45 help="sampe -a; This option is only used when there are not enough good alignment to infer the distribution of insert sizes; default=500"/> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
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46 <param name="o" type="integer" value="100000" |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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47 label="Maximum occurrences of a read for pairing. A read with more occurrences will be treated as a single-end read." |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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48 help="sampe -o; Reducing this parameter helps faster pairing; default=100000"/> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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changeset
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49 <param name="n" type="integer" value="3" |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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50 label="Maximum number of alignments to output in the XA tag for reads paired properly." |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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51 help="sampe -n; If a read has more than this many hits, the XA tag will not be written; default=3"/> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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52 <param name="N" type="integer" value="10" |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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53 label="Maximum number of alignments to output in the XA tag for disconcordant read pairs (excluding singletons)." |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
diff
changeset
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54 help="sampe -N; If a read has more than this many hits, the XA tag will not be written; default=10"/> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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55 <param name="c" type="float" value="0.00005" label="Prior of chimeric rate (lower bound)" |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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56 help="sampe -c"/> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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57 </when> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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diff
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58 <when value="do_not_set"> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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59 <!-- do nothing --> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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60 </when> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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61 </xml> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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62 <xml name="advanced_se_options"> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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63 <param name="adv_se_options_selector" type="select" label="Set advanced single end options?" |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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64 help="Provides additional controls"> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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65 <option value="set">Set</option> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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66 <option value="do_not_set" selected="True">Do not set</option> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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67 </param> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
14
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68 <when value="set"> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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diff
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69 <param name="n" type="integer" value="3" label="Maximum number of alignments to output in the XA tag." |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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70 help="-n; If a read has more than this many hits, the XA tag will not be written; default=3"/> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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71 </when> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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72 <when value="do_not_set"> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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73 <!-- do nothing --> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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74 </when> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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75 </xml> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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76 </macros> |
26
2477830927ec
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 6e9628b1d92fdb358b79959ad54a456cfa46fa33
iuc
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77 <expand macro="bio_tools"/> |
18
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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78 <expand macro="requirements"/> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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79 <expand macro="stdio"/> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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80 <command> |
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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81 <![CDATA[ |
21
8d2a528a9513
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 9bbf35d7f47e5ab6b78248c9907b71446d4e7b0c
iuc
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82 @pipefail@ |
18
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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83 @set_reference_fasta_filename@ |
2 | 84 |
18
48f306c57611
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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85 ## setup vars for rg handling... |
48f306c57611
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86 @define_read_group_helpers@ |
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87 #if str( $input_type.input_type_selector ) == "paired": |
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88 #set $rg_auto_name = $read_group_name_default($input_type.fastq_input1, $input_type.fastq_input2) |
48f306c57611
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89 #elif str( $input_type.input_type_selector ) in ["single_bam", "paired_bam"]: |
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90 #set $rg_auto_name = $read_group_name_default($input_type.bam_input) |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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91 #else |
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92 #set $rg_auto_name = $read_group_name_default($input_type.fastq_input1) |
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93 #end if |
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94 @set_use_rg_var@ |
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95 @set_read_group_vars@ |
2 | 96 |
18
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97 ## Begin bwa command line |
2 | 98 |
99 ####### Fastq paired | |
100 | |
18
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101 #if str( $input_type.input_type_selector ) == "paired" or str( $input_type.input_type_selector ) == "paired_collection": |
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102 bwa aln |
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103 -t "\${GALAXY_SLOTS:-1}" |
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104 @command_options@ |
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105 '$reference_fasta_filename' |
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106 #if str( $input_type.input_type_selector ) == "paired_collection": |
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107 '${input_type.fastq_input1.forward}' |
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108 #else |
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109 '${input_type.fastq_input1}' |
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110 #end if |
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111 > first.sai && |
2 | 112 |
18
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113 bwa aln |
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114 -t "\${GALAXY_SLOTS:-1}" |
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115 @command_options@ |
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116 '${reference_fasta_filename}' |
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117 #if str( $input_type.input_type_selector ) == "paired_collection": |
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118 '${input_type.fastq_input1.reverse}' |
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119 #else |
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120 '${input_type.fastq_input2}' |
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121 #end if |
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122 > second.sai && |
2 | 123 |
18
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124 bwa sampe |
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125 #if str( $input_type.adv_pe_options.adv_pe_options_selector) == "True": |
2 | 126 -a ${$input_type.adv_pe_options.a} |
127 -o ${$input_type.adv_pe_options.o} | |
128 -n ${$input_type.adv_pe_options.n} | |
129 -N ${$input_type.adv_pe_options.N} | |
25
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130 -c ${$input_type.adv_pe_options.c} |
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131 #end if |
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132 @read_group_options@ |
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133 #if str( $input_type.input_type_selector ) == "paired_collection": |
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134 '${reference_fasta_filename}' first.sai second.sai '${input_type.fastq_input1.forward}' '${input_type.fastq_input1.reverse}' |
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135 #else: |
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136 '${reference_fasta_filename}' first.sai second.sai '${input_type.fastq_input1}' '${input_type.fastq_input2}' |
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137 #end if |
2 | 138 |
18
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139 ## Fastq single |
2 | 140 |
18
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141 #elif str( $input_type.input_type_selector ) == "single": |
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142 bwa aln |
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143 -t "\${GALAXY_SLOTS:-1}" |
2 | 144 |
18
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145 @command_options@ |
2 | 146 |
18
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147 '${reference_fasta_filename}' |
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148 '${input_type.fastq_input1}' |
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149 > first.sai && |
2 | 150 |
18
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151 bwa samse |
2 | 152 |
18
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153 #if str( $input_type.adv_se_options.adv_se_options_selector) == "True": |
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154 -n ${$input_type.adv_se_options.n} |
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155 #end if |
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156 @read_group_options@ |
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157 '${reference_fasta_filename}' first.sai '${input_type.fastq_input1}' |
2 | 158 |
159 ####### BAM paired | |
160 | |
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161 #elif str( $input_type.input_type_selector ) == "paired_bam": |
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162 bwa aln |
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163 -t "\${GALAXY_SLOTS:-1}" |
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164 -b |
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165 -1 |
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166 @command_options@ |
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167 '${reference_fasta_filename}' |
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168 '${input_type.bam_input}' |
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169 > first.sai && |
2 | 170 |
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171 bwa aln |
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172 -t "\${GALAXY_SLOTS:-1}" |
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173 -b |
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174 -2 |
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175 @command_options@ |
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176 '${reference_fasta_filename}' |
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177 '${input_type.bam_input}' |
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178 > second.sai && |
2 | 179 |
18
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180 bwa sampe |
2 | 181 |
18
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182 #if str( $input_type.adv_bam_pe_options.adv_pe_options_selector) == "True": |
2 | 183 -a ${$input_type.adv_bam_pe_options.a} |
184 -o ${$input_type.adv_bam_pe_options.o} | |
185 -n ${$input_type.adv_bam_pe_options.n} | |
186 -N ${$input_type.adv_bam_pe_options.N} | |
18
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187 #end if |
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188 @read_group_options@ |
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189 '${reference_fasta_filename}' first.sai second.sai '${input_type.bam_input}' '${input_type.bam_input}' |
2 | 190 |
191 ####### Fastq single ------------ to do next | |
192 | |
18
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193 #elif str( $input_type.input_type_selector ) == "single_bam": |
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194 bwa aln |
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195 -t "\${GALAXY_SLOTS:-1}" |
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196 -b |
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197 -0 |
2 | 198 |
18
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199 @command_options@ |
2 | 200 |
18
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201 '${reference_fasta_filename}' |
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202 '${input_type.bam_input}' |
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203 > first.sai && |
2 | 204 |
18
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205 bwa samse |
2 | 206 |
18
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207 #if str( $input_type.adv_bam_se_options.adv_se_options_selector) == "True": |
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208 -n ${$input_type.adv_bam_se_options.n} |
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209 #end if |
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210 @read_group_options@ |
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211 '${reference_fasta_filename}' first.sai '${input_type.bam_input}' |
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212 #end if |
2 | 213 |
23
3fe632431b68
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 777181bd04849dab271ee2cc95e45c182947d308"
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214 | samtools sort -@\${GALAXY_SLOTS:-2} -T "\${TMPDIR:-.}" -O bam -o '$bam_output' |
18
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215 ]]> |
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216 </command> |
2 | 217 |
18
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218 <inputs> |
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219 <expand macro="reference_source_conditional"/> |
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220 <conditional name="input_type"> |
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221 <param name="input_type_selector" type="select" label="Select input type" |
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222 help="Select between fastq and bam datasets and between paired and single end data"> |
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223 <option value="paired">Paired fastq</option> |
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224 <option value="paired_collection">Paired fastq collection</option> |
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225 <option value="single">Single fastq</option> |
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226 <option value="paired_bam">Paired BAM</option> |
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227 <option value="single_bam">Single BAM</option> |
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228 </param> |
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229 <when value="paired"> |
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230 <param name="fastq_input1" type="data" format="fastqsanger,fastqsanger.gz,fasta" |
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231 label="Select first set of reads" help="Specify dataset with forward reads"/> |
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232 <param name="fastq_input2" type="data" format="fastqsanger,fastqsanger.gz,fasta" |
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233 label="Select second set of reads" help="Specify dataset with reverse reads"/> |
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234 <conditional name="adv_pe_options"> |
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235 <expand macro="advanced_pe_options"/> |
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236 </conditional> |
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237 </when> |
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238 <when value="paired_collection"> |
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239 <param name="fastq_input1" format="fastqsanger,fastqsanger.gz" type="data_collection" collection_type="paired" label="Select a paired collection" |
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240 help="See help section for an explanation of dataset collections"/> |
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241 <conditional name="adv_pe_options"> |
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242 <expand macro="advanced_pe_options"/> |
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243 </conditional> |
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244 </when> |
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245 <when value="single"> |
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246 <param name="fastq_input1" type="data" format="fastqsanger,fastqsanger.gz,fasta" |
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247 label="Select fastq dataset" help="Specify dataset with single reads"/> |
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248 <conditional name="adv_se_options"> |
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249 <expand macro="advanced_se_options"/> |
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250 </conditional> |
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251 </when> |
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252 <!-- the difference between single and paired bams is in the <command> tag portion and realated to -0, -1, and -2 options --> |
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253 <when value="paired_bam"> |
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254 <param name="bam_input" type="data" format="bam" label="Select BAM dataset" |
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255 help="Specify BAM dataset with paired reads"/> |
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256 <conditional name="adv_bam_pe_options"> |
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257 <expand macro="advanced_pe_options"/> |
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258 </conditional> |
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259 </when> |
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260 <when value="single_bam"> |
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261 <param name="bam_input" type="data" format="bam" label="Select BAM dataset" |
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262 help="Specify BAM dataset with single reads"/> |
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263 <conditional name="adv_bam_se_options"> |
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264 <expand macro="advanced_se_options"/> |
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265 </conditional> |
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266 </when> |
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267 </conditional> |
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268 <expand macro="read_group_conditional"/> |
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269 <conditional name="analysis_type"> |
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270 <param name="analysis_type_selector" type="select" label="Select analysis mode"> |
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271 <option value="illumina">1.Simple Illumina mode</option> |
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272 <option value="full">2.Full list of options</option> |
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273 </param> |
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274 <when value="illumina"> |
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275 <!-- do nothing --> |
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276 </when> |
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277 <when value="full"> |
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278 <param name="n" type="text" value="0.04" |
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279 label="maximum edit distance if the value is integer, or the fraction of missing alignments given 2% uniform base error rate if float. In the latter case, the maximum edit distance is automatically chosen for different read lengths." |
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280 help="aln -n; default=0.04"/> |
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281 <param name="o" type="integer" value="1" label="maximum number or gap openings" |
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282 help="aln -o; default=1"/> |
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283 <param name="e" type="integer" value="-1" label="maximum number of gap extensions" |
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284 help="aln -e; -1 disables long gaps and invokes k-difference mode; default=-1"/> |
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285 <param name="i" type="integer" value="5" |
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286 label="do not put an indel within this many bp towards the ends" help="aln -i; default=5"/> |
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287 <param name="d" type="integer" value="10" label="maximum occurrences for extending a long deletion" |
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288 help="aln -d; default=10"/> |
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289 <param name="l" type="integer" value="32" label="seed length" help="aln -l; default=32"/> |
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290 <param name="k" type="integer" value="2" label="maximum differences in the seed" |
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291 help="aln -k; default=2"/> |
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292 <param name="m" type="integer" value="2000000" label="maximum entries in the queue" |
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293 help="aln -m; default=2000000"/> |
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294 <param name="M" type="integer" value="3" label="mismatch penalty" help="aln -M; default=3"/> |
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295 <param name="O" type="integer" value="11" label="gap open penalty" help="aln -O; default=11"/> |
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296 <param name="E" type="integer" value="4" label="gap extension penalty" help="aln -E; default=4"/> |
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297 <param name="R" type="integer" value="30" |
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298 label="stop searching when there are more than this value of equally best hits" |
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299 help="aln -R; default=30"/> |
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300 <param name="q" type="integer" value="0" label="quality threshold for read trimming down to 35bp" |
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301 help="aln -q; default=0"/> |
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302 <param name="B" type="integer" optional="True" label="length of barcode" |
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303 help="aln -B; optional parameter"/> |
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304 <param name="L" type="float" optional="True" label="log-scaled gap penalty for long deletions" |
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305 help="aln -L; optional parameter"/> |
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306 </when> |
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307 </conditional> |
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308 </inputs> |
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309 <outputs> |
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310 <data format="bam" name="bam_output" label="${tool.name} on ${on_string} (mapped reads in BAM format)"> |
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311 <expand macro="dbKeyActionsBwaMem"/> |
18
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312 </data> |
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313 </outputs> |
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314 <tests> |
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315 <!-- `samtools sort` in the new update adds PG lines to the output so the lines_diff is changed from "2" to "4" --> |
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316 <test expect_num_outputs="1"> |
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317 <param name="reference_source_selector" value="history"/> |
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318 <param name="ref_file" ftype="fasta" value="bwa-mem-mt-genome.fa"/> |
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319 <param name="input_type_selector" value="single"/> |
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320 <param name="fastq_input1" ftype="fasta" value="bwa-mem-fasta1.fa"/> |
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321 <param name="analysis_type_selector" value="illumina"/> |
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322 <output name="bam_output" ftype="bam" file="bwa-aln-test1-fasta.bam" lines_diff="4"/> |
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323 </test> |
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324 <test expect_num_outputs="1"> |
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325 <param name="reference_source_selector" value="history"/> |
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326 <param name="ref_file" ftype="fasta" value="bwa-mem-mt-genome.fa"/> |
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327 <param name="input_type_selector" value="paired"/> |
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328 <param name="fastq_input1" ftype="fastqsanger" value="bwa-mem-fastq1.fq"/> |
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329 <param name="fastq_input2" ftype="fastqsanger" value="bwa-mem-fastq2.fq"/> |
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330 <param name="analysis_type_selector" value="illumina"/> |
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331 <output name="bam_output" ftype="bam" file="bwa-aln-test1.bam" lines_diff="4"/> |
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332 </test> |
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333 <test expect_num_outputs="1"> |
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334 <param name="reference_source_selector" value="history"/> |
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335 <param name="ref_file" ftype="fasta" value="bwa-mem-mt-genome.fa"/> |
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336 <param name="input_type_selector" value="paired"/> |
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337 <param name="fastq_input1" ftype="fastqsanger.gz" value="bwa-mem-fastq1.fq.gz"/> |
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338 <param name="fastq_input2" ftype="fastqsanger" value="bwa-mem-fastq2.fq"/> |
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339 <param name="analysis_type_selector" value="illumina"/> |
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340 <output name="bam_output" ftype="bam" file="bwa-aln-test1.bam" lines_diff="4"/> |
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341 </test> |
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342 <test expect_num_outputs="1"> |
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343 <param name="reference_source_selector" value="history"/> |
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344 <param name="ref_file" ftype="fasta" value="bwa-mem-mt-genome.fa"/> |
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345 <param name="input_type_selector" value="paired_bam"/> |
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346 <param name="bam_input" ftype="bam" value="bwa-aln-bam-input.bam"/> |
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347 <param name="analysis_type_selector" value="illumina"/> |
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348 <output name="bam_output" ftype="bam" file="bwa-aln-test2.bam" lines_diff="4"/> |
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349 </test> |
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350 <test expect_num_outputs="1"> |
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351 <param name="reference_source_selector" value="history"/> |
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352 <param name="ref_file" ftype="fasta" value="bwa-mem-mt-genome.fa"/> |
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353 <param name="input_type_selector" value="paired"/> |
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354 <param name="fastq_input1" ftype="fastqsanger" value="bwa-mem-fastq1.fq"/> |
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355 <param name="fastq_input2" ftype="fastqsanger" value="bwa-mem-fastq2.fq"/> |
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356 <param name="rg_selector" value="set"/> |
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357 <param name="ID" value="rg1"/> |
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358 <param name="PL" value="CAPILLARY"/> |
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359 <param name="analysis_type_selector" value="illumina"/> |
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360 <output name="bam_output" ftype="bam" file="bwa-aln-test3.bam" lines_diff="5"/> |
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361 </test> |
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362 </tests> |
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363 <help><![CDATA[ |
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364 **What it does** |
0 | 365 |
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366 BWA_ is a software package for mapping low-divergent sequences against a large reference genome, such as the human genome. |
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367 The bwa-aln algorithm is designed for Illumina sequence reads up to 100bp. For longer reads use the separate BWA-MEM Galaxy tool. |
0 | 368 |
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369 This Galaxy tool wraps the bwa-aln, bwa-samse and -sampe modules of the BWA read mapping tool: |
0 | 370 |
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371 - **bwa aln** - actual mapper placing reads onto the reference sequence |
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372 - **bwa samse** - post-processor converting suffix array coordinates into genome coordinates in SAM format for |
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373 single reads |
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374 - **bam sampe** - post-processor for paired reads |
2 | 375 |
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376 For more details about the different modules of the BWA package see the `BWA manual`_. |
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377 |
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378 The Galaxy implementation takes fastq or BAM (unaligned BAM) datasets as input and produces output in BAM format, |
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379 which can be further processed using various BAM utilities existing in Galaxy (BAMTools, SAMTools, Picard). |
0 | 380 |
381 ----- | |
382 | |
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383 @ref_genomes@ |
0 | 384 |
385 @RG@ | |
386 | |
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387 @links@ |
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388 ]]></help> |
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389 <citations> |
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390 <citation type="doi">10.1093/bioinformatics/btp324</citation> |
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391 <citation type="doi">10.1093/bioinformatics/btp698</citation> |
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392 </citations> |
0 | 393 </tool> |