# HG changeset patch # User devteam # Date 1487524325 18000 # Node ID e66b9b5b8580df969f96c418daff7c68134501c5 # Parent 1322b73ffe4485cf16e859dd61e22f2b136401a9 planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/cufflinks/cuffcompare commit 82ee6fc860c52c531b7a57bbb346ab1a67a434a5 diff -r 1322b73ffe44 -r e66b9b5b8580 cuff_macros.xml --- a/cuff_macros.xml Tue Feb 07 18:38:41 2017 -0500 +++ b/cuff_macros.xml Sun Feb 19 12:12:05 2017 -0500 @@ -8,14 +8,6 @@ - - - - - - - - diff -r 1322b73ffe44 -r e66b9b5b8580 cuffcompare_wrapper.py --- a/cuffcompare_wrapper.py Tue Feb 07 18:38:41 2017 -0500 +++ b/cuffcompare_wrapper.py Sun Feb 19 12:12:05 2017 -0500 @@ -38,23 +38,6 @@ (options, args) = parser.parse_args() - # output version # of tool - try: - with tempfile.NamedTemporaryFile() as tmp_stdout: - returncode = subprocess.call(args='cuffcompare 2>&1', stdout=tmp_stdout, shell=True) - stdout = None - with open(tmp_stdout.name) as tmp_stdout2: - for line in tmp_stdout2: - if line.lower().find('cuffcompare v') >= 0: - stdout = line.strip() - break - if stdout: - sys.stdout.write('%s\n' % stdout) - else: - raise Exception - except: - sys.stdout.write('Could not determine Cuffcompare version\n') - # Set/link to sequence file. if options.use_seq_data: if options.ref_file: diff -r 1322b73ffe44 -r e66b9b5b8580 cuffcompare_wrapper.xml --- a/cuffcompare_wrapper.xml Tue Feb 07 18:38:41 2017 -0500 +++ b/cuffcompare_wrapper.xml Sun Feb 19 12:12:05 2017 -0500 @@ -1,12 +1,11 @@ - + compare assembled transcripts to a reference annotation and track Cufflinks transcripts across multiple experiments cuff_macros.xml - cuffcompare 2>&1 | head -n 1 - + + ]]> @@ -60,7 +59,7 @@ - @@ -91,29 +90,29 @@ - - - - - annotation['use_ref_annotation'] == 'Yes' @HAS_MULTIPLE_INPUTS@ - annotation['use_ref_annotation'] == 'Yes' and @HAS_MULTIPLE_INPUTS@ @@ -124,7 +123,7 @@ - @@ -173,7 +172,7 @@ Cuffcompare takes Cufflinks' GTF output as input, and optionally can take a "reference" annotation (such as from Ensembl_) -.. _Ensembl: http://www.ensembl.org +.. _Ensembl: http://www.ensembl.org ------ @@ -187,7 +186,7 @@ Transcripts Combined File: -Cuffcompare reports a GTF file containing the "union" of all transfrags in each sample. If a transfrag is present in both samples, it is thus reported once in the combined gtf. +Cuffcompare reports a GTF file containing the "union" of all transfrags in each sample. If a transfrag is present in both samples, it is thus reported once in the combined gtf. Transcripts Tracking File: @@ -209,7 +208,7 @@ 3 Reference gene id Tcea The gene_name attribute of the reference GTF record for this transcript, or '-' if no reference transcript overlaps this Cufflinks transcript 4 Reference transcript id uc007afj.1 The transcript_id attribute of the reference GTF record for this transcript, or '-' if no reference transcript overlaps this Cufflinks transcript 5 Class code c The type of match between the Cufflinks transcripts in column 6 and the reference transcript. See class codes - + Each of the columns after the fifth have the following format: qJ:gene_id|transcript_id|FMI|FPKM|conf_lo|conf_hi @@ -222,18 +221,18 @@ Priority Code Description --------------------------------- 1 = Match - 2 c Contained - 3 j New isoform - 4 e A single exon transcript overlapping a reference exon and at least 10 bp of a reference intron, indicating a possible pre-mRNA fragment. + 2 c Contained + 3 j New isoform + 4 e A single exon transcript overlapping a reference exon and at least 10 bp of a reference intron, indicating a possible pre-mRNA fragment. 5 i A single exon transcript falling entirely with a reference intron 6 o Generic exonic overlap with a reference transcript - 7 p Possible polymerase run-on fragment - 8 r Repeat. Currently determined by looking at the soft-masked reference sequence and applied to transcripts where at least 50% of the bases are lower case + 7 p Possible polymerase run-on fragment + 8 r Repeat. Currently determined by looking at the soft-masked reference sequence and applied to transcripts where at least 50% of the bases are lower case 9 u Unknown, intergenic transcript 10 x Exonic overlap with reference on the opposite strand 11 s An intron of the transfrag overlaps a reference intron on the opposite strand (likely due to read mapping errors) 12 . (.tracking file only, indicates multiple classifications) - + ------- **Settings** diff -r 1322b73ffe44 -r e66b9b5b8580 tool_dependencies.xml --- a/tool_dependencies.xml Tue Feb 07 18:38:41 2017 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,6 +0,0 @@ - - - - - -