Mercurial > repos > devteam > cuffmerge
diff cuffmerge_wrapper.py @ 2:5b285b6e4ee3
Update to the new data table specification.
author | Dave Bouvier <dave@bx.psu.edu> |
---|---|
date | Wed, 04 Dec 2013 13:24:59 -0500 |
parents | dbbd37e013aa |
children | b6e3849293b1 |
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--- a/cuffmerge_wrapper.py Tue Oct 01 15:13:24 2013 -0400 +++ b/cuffmerge_wrapper.py Wed Dec 04 13:24:59 2013 -0500 @@ -8,20 +8,6 @@ sys.stderr.write( '%s\n' % msg ) sys.exit() -# Copied from sam_to_bam.py: -def check_seq_file( dbkey, cached_seqs_pointer_file ): - seq_path = '' - for line in open( cached_seqs_pointer_file ): - line = line.rstrip( '\r\n' ) - if line and not line.startswith( '#' ) and line.startswith( 'index' ): - fields = line.split( '\t' ) - if len( fields ) < 3: - continue - if fields[1] == dbkey: - seq_path = fields[2].strip() - break - return seq_path - def __main__(): #Parse Command Line parser = optparse.OptionParser() @@ -31,8 +17,7 @@ # Wrapper / Galaxy options. - parser.add_option( '', '--dbkey', dest='dbkey', help='The build of the reference dataset' ) - parser.add_option( '', '--index_dir', dest='index_dir', help='GALAXY_DATA_INDEX_DIR' ) + parser.add_option( '', '--index', dest='index', help='The path of the reference genome' ) parser.add_option( '', '--ref_file', dest='ref_file', help='The reference dataset from the history' ) # Outputs. @@ -61,21 +46,15 @@ # Set/link to sequence file. if options.use_seq_data: - if options.ref_file != 'None': + if options.ref_file: # Sequence data from history. # Create symbolic link to ref_file so that index will be created in working directory. seq_path = "ref.fa" os.symlink( options.ref_file, seq_path ) else: - # Sequence data from loc file. - cached_seqs_pointer_file = os.path.join( options.index_dir, 'sam_fa_indices.loc' ) - if not os.path.exists( cached_seqs_pointer_file ): - stop_err( 'The required file (%s) does not exist.' % cached_seqs_pointer_file ) - # If found for the dbkey, seq_path will look something like /galaxy/data/equCab2/sam_index/equCab2.fa, - # and the equCab2.fa file will contain fasta sequences. - seq_path = check_seq_file( options.dbkey, cached_seqs_pointer_file ) - if seq_path == '': - stop_err( 'No sequence data found for dbkey %s, so sequence data cannot be used.' % options.dbkey ) + if not os.path.exists( options.index ): + stop_err( 'Reference genome %s not present, request it by reporting this error.' % options.index ) + seq_path = options.index # Build command.