Mercurial > repos > devteam > cummerbund
diff test-data/heatmap.txt @ 6:c3b54a4b7741 draft default tip
"planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/cummerbund commit f929353ffb0623f2218d7dec459c7da62f3b0d24"
author | devteam |
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date | Mon, 06 Jul 2020 20:25:47 -0400 |
parents | 78fcfc04fcfe |
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--- a/test-data/heatmap.txt Thu Feb 23 20:24:03 2017 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,311 +0,0 @@ -> get_features <- function(myGenes, f="gene") { -+ if (f == "isoforms") -+ return(isoforms(myGenes)) -+ else if (f == "tss") -+ return(TSS(myGenes)) -+ else if (f == "cds") -+ return(CDS(myGenes)) -+ else -+ return(myGenes) -+ } -> -> ## Main Function ## -> -> library(argparse) -Loading required package: proto -> -> parser <- ArgumentParser(description='Create a plot with cummeRbund') -> -> parser$add_argument('--type', dest='plotType', default='Density', required=TRUE) -> parser$add_argument('--height', dest='height', type='integer', default=960, required=TRUE) -> parser$add_argument('--width', dest='width', type='integer', default=1280, required=TRUE) -> parser$add_argument('--outfile', dest='filename', default="plot-unknown-0.png", required=TRUE) -> parser$add_argument('--input', dest='input_database', default="cuffData.db", required=TRUE) -> parser$add_argument('--smooth', dest='smooth', action="store_true", default=FALSE) -> parser$add_argument('--gene_selector', dest='gene_selector', action="store_true", default=FALSE) -> parser$add_argument('--replicates', dest='replicates', action="store_true", default=FALSE) -> parser$add_argument('--labcol', dest='labcol', action="store_true", default=FALSE) -> parser$add_argument('--labrow', dest='labrow', action="store_true", default=FALSE) -> parser$add_argument('--border', dest='border', action="store_true", default=FALSE) -> parser$add_argument('--summary', dest='summary', action="store_true", default=FALSE) -> parser$add_argument('--count', dest='count', action="store_true", default=FALSE) -> parser$add_argument('--error_bars', dest='error_bars', action="store_true", default=FALSE) -> parser$add_argument('--log10', dest='log10', action="store_true", default=FALSE) -> parser$add_argument('--features', dest='features', action="store", default="genes") -> parser$add_argument('--clustering', dest='clustering', action="store", default="both") -> parser$add_argument('--iter_max', dest='iter_max', action="store") -> parser$add_argument('--genes', dest='genes', action="append") -> parser$add_argument('--k', dest='k', action="store") -> parser$add_argument('--x', dest='x', action="store") -> parser$add_argument('--y', dest='y', action="store") -> -> args <- parser$parse_args() -> -> ## Load cummeRbund library -> library("cummeRbund") -Loading required package: BiocGenerics -Loading required package: methods -Loading required package: parallel - -Attaching package: ‘BiocGenerics’ - -The following objects are masked from ‘package:parallel’: - - clusterApply, clusterApplyLB, clusterCall, clusterEvalQ, - clusterExport, clusterMap, parApply, parCapply, parLapply, - parLapplyLB, parRapply, parSapply, parSapplyLB - -The following objects are masked from ‘package:stats’: - - IQR, mad, xtabs - -The following objects are masked from ‘package:base’: - - anyDuplicated, append, as.data.frame, cbind, colnames, do.call, - duplicated, eval, evalq, Filter, Find, get, grep, grepl, intersect, - is.unsorted, lapply, lengths, Map, mapply, match, mget, order, - paste, pmax, pmax.int, pmin, pmin.int, Position, rank, rbind, - Reduce, rownames, sapply, setdiff, sort, table, tapply, union, - unique, unsplit, which, which.max, which.min - -Loading required package: RSQLite -Loading required package: DBI -Loading required package: ggplot2 -Loading required package: reshape2 -Loading required package: fastcluster - -Attaching package: ‘fastcluster’ - -The following object is masked from ‘package:stats’: - - hclust - -Loading required package: rtracklayer -Loading required package: GenomicRanges -Loading required package: stats4 -Loading required package: S4Vectors - -Attaching package: ‘S4Vectors’ - -The following objects are masked from ‘package:base’: - - colMeans, colSums, expand.grid, rowMeans, rowSums - -Loading required package: IRanges -Loading required package: GenomeInfoDb -Loading required package: Gviz -Loading required package: grid -Warning: namespace ‘AnnotationHub’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘AnnotationHub’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘AnnotationHub’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘AnnotationHub’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘AnnotationHub’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘AnnotationHub’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘AnnotationHub’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘AnnotationHub’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘AnnotationHub’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘AnnotationHub’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘ensembldb’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘AnnotationHub’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ -Warning: namespace ‘AnnotationHub’ is not available and has been replaced -by .GlobalEnv when processing object ‘plot.index’ - -Attaching package: ‘cummeRbund’ - -The following object is masked from ‘package:GenomicRanges’: - - promoters - -The following object is masked from ‘package:IRanges’: - - promoters - -The following object is masked from ‘package:BiocGenerics’: - - conditions - -> -> ## Initialize cuff object -> cuff <- readCufflinks(dir = "", dbFile = args$input_database, rebuild = FALSE) -> -> ## Print out info -> print(cuff) -CuffSet instance with: - 2 samples - 87 genes - 90 isoforms - 88 TSS - 0 CDS - 87 promoters - 88 splicing - 0 relCDS -> sink("cuffdb_info.txt") -> print(cuff) -> print("SAMPLES:") -> samples(cuff) -> print("REPLICATES:") -> replicates(cuff) -> print("FEATURES:") -> print(annotation(genes(cuff))) -> cat(annotation(genes(cuff))[[1]],sep=",") -> sink() -> -> png(filename = args$filename, width = args$width, height = args$height, type=c('cairo-png')) -> tryCatch({ -+ if (args$plotType == 'density') { -+ csDensity(genes(cuff), replicates=args$replicates, logMode=args$log10) -+ } -+ else if (args$plotType == 'boxplot') { -+ csBoxplot(genes(cuff), replicates=args$replicates, logMode=args$log10) -+ } -+ else if (args$plotType == 'mds') { -+ MDSplot(genes(cuff), replicates=args$replicates) -+ } -+ else if (args$plotType == 'pca') { -+ PCAplot(genes(cuff), "PC1", "PC2", replicates=args$replicates) -+ } -+ else if (args$plotType == 'dendrogram') { -+ csDendro(genes(cuff), replicates=args$replicates) -+ } -+ else if (args$plotType == 'scatter') { -+ if (args$gene_selector) { -+ myGenes <- getGenes(cuff, args$genes) -+ csScatter(get_features(myGenes, args$features), args$x, args$y, smooth=args$smooth, logMode=args$log10) -+ } -+ else { -+ csScatter(genes(cuff), args$x, args$y, smooth=args$smooth, logMode=args$log10) -+ } -+ } -+ else if (args$plotType == 'volcano') { -+ if (args$gene_selector) { -+ myGenes <- get_features(getGenes(cuff, args$genes), args$features) -+ } -+ else { -+ myGenes <- genes(cuff) -+ } -+ csVolcano(myGenes, args$x, args$y) -+ } -+ else if (args$plotType == 'heatmap') { -+ if (args$gene_selector) { -+ myGenes <- getGenes(cuff, args$genes) -+ } -+ else { -+ myGenes <- getGenes(cuff,annotation(genes(cuff))[[1]]) -+ } -+ csHeatmap(get_features(myGenes, args$features), clustering=args$clustering, labCol=args$labcol, labRow=args$labrow, border=args$border, logMode=args$log10) -+ } -+ else if (args$plotType == 'cluster') { -+ myGenes <- getGenes(cuff, args$genes) -+ csCluster(get_features(myGenes, args$features), k=args$k) -+ } -+ else if (args$plotType == 'dispersion') { -+ dispersionPlot(genes(cuff)) -+ } -+ else if (args$plotType == 'fpkmSCV') { -+ fpkmSCVPlot(genes(cuff)) -+ } -+ else if (args$plotType == 'scatterMatrix') { -+ csScatterMatrix(genes(cuff)) -+ } -+ else if (args$plotType == 'expressionplot') { -+ myGenes <- getGenes(cuff, args$genes) -+ expressionPlot(get_features(myGenes, args$features), drawSummary=args$summary, showErrorbars=args$error_bars, replicates=args$replicates) -+ } -+ else if (args$plotType == 'expressionbarplot') { -+ myGeneId <- args$genes -+ myGenes <- getGenes(cuff, myGeneId) -+ expressionBarplot(get_features(myGenes, args$features), showErrorbars=args$error_bars, replicates=args$replicates) -+ } -+ else if (args$plotType == 'mds') { -+ MDSplot(genes(cuff),replicates=args$replicates) -+ } -+ else if (args$plotType == 'pca') { -+ PCAplot(genes(cuff),"PC1","PC2", replicates=args$replicates) -+ } -+ else if (args$plotType == 'maplot') { -+ MAplot(genes(cuff), args$x, args$y, useCount=args$count) -+ } -+ else if (args$plotType == 'genetrack') { -+ myGene <- getGene(cuff, args$genes) -+ plotTracks(makeGeneRegionTrack(myGene)) -+ } -+ },error = function(e) { -+ write(paste("Failed:", e, sep=" "), stderr()) -+ q("no", 1, TRUE) -+ }) -Getting gene information: - FPKM - Differential Expression Data - Annotation Data - Replicate FPKMs - Counts -Getting isoforms information: - FPKM - Differential Expression Data - Annotation Data - Replicate FPKMs - Counts -Getting CDS information: - FPKM - Differential Expression Data - Annotation Data - Replicate FPKMs - Counts -Getting TSS information: - FPKM - Differential Expression Data - Annotation Data - Replicate FPKMs - Counts -Getting promoter information: - distData -Getting splicing information: - distData -Getting relCDS information: - distData -Using tracking_id, sample_name as id variables -No id variables; using all as measure variables -> devname = dev.off() ->