Mercurial > repos > devteam > data_manager_bowtie2_index_builder
view data_manager/bowtie2_index_builder.py @ 10:9dd107db92c2 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/data_managers/data_manager_bowtie2_index_builder commit 096286097ed5cdf189a1b68c3fc34d10f4142e54
author | iuc |
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date | Sun, 16 Apr 2023 08:29:03 +0000 |
parents | 1586b111d2a3 |
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#!/usr/bin/env python # Dan Blankenberg from __future__ import print_function import json import optparse import os import subprocess import sys DEFAULT_DATA_TABLE_NAMES = ["bowtie2_indexes"] def get_id_name(params, dbkey, fasta_description=None): # TODO: ensure sequence_id is unique and does not already appear in location file sequence_id = params['param_dict']['sequence_id'] if not sequence_id: sequence_id = dbkey sequence_name = params['param_dict']['sequence_name'] if not sequence_name: sequence_name = fasta_description if not sequence_name: sequence_name = dbkey return sequence_id, sequence_name def build_bowtie2_index(data_manager_dict, fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_names=DEFAULT_DATA_TABLE_NAMES): # TODO: allow multiple FASTA input files fasta_base_name = os.path.split(fasta_filename)[-1] sym_linked_fasta_filename = os.path.join(target_directory, fasta_base_name) os.symlink(fasta_filename, sym_linked_fasta_filename) args = ['bowtie2-build', sym_linked_fasta_filename, sequence_id] threads = os.environ.get('GALAXY_SLOTS') if threads: args.extend(['--threads', threads]) proc = subprocess.Popen(args=args, shell=False, cwd=target_directory) return_code = proc.wait() if return_code: print("Error building index.", file=sys.stderr) sys.exit(return_code) data_table_entry = dict(value=sequence_id, dbkey=dbkey, name=sequence_name, path=sequence_id) for data_table_name in data_table_names: _add_data_table_entry(data_manager_dict, data_table_name, data_table_entry) def _add_data_table_entry(data_manager_dict, data_table_name, data_table_entry): data_manager_dict['data_tables'] = data_manager_dict.get('data_tables', {}) data_manager_dict['data_tables'][data_table_name] = data_manager_dict['data_tables'].get(data_table_name, []) data_manager_dict['data_tables'][data_table_name].append(data_table_entry) return data_manager_dict def main(): parser = optparse.OptionParser() parser.add_option('-f', '--fasta_filename', dest='fasta_filename', action='store', type="string", default=None, help='fasta_filename') parser.add_option('-d', '--fasta_dbkey', dest='fasta_dbkey', action='store', type="string", default=None, help='fasta_dbkey') parser.add_option('-t', '--fasta_description', dest='fasta_description', action='store', type="string", default=None, help='fasta_description') parser.add_option('-n', '--data_table_name', dest='data_table_name', action='append', type="string", default=None, help='data_table_name') (options, args) = parser.parse_args() filename = args[0] with open(filename) as fh: params = json.load(fh) target_directory = params['output_data'][0]['extra_files_path'] os.mkdir(target_directory) data_manager_dict = {} dbkey = options.fasta_dbkey if dbkey in [None, '', '?']: raise Exception('"%s" is not a valid dbkey. You must specify a valid dbkey.' % (dbkey)) sequence_id, sequence_name = get_id_name(params, dbkey=dbkey, fasta_description=options.fasta_description) # build the index build_bowtie2_index(data_manager_dict, options.fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_names=options.data_table_name or DEFAULT_DATA_TABLE_NAMES) # save info to json file with open(filename, 'w') as fh: json.dump(data_manager_dict, fh, sort_keys=True) if __name__ == "__main__": main()