Mercurial > repos > devteam > draw_stacked_barplots

comparison draw_stacked_barplots.pl @ 0:063ecbe59faf draft

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author devteam
date Wed, 25 Sep 2013 10:29:15 -0400
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1 #!/usr/bin/perl -w
2
3 # This program draws, in a pdf file, a stacked bars plot for different categories of data and for
4 # different criteria. For each criterion a stacked bar is drawn, such that the height of each stacked
5 # sub-bar represents the number of elements in each category satisfying that criterion.
6 # The input consists of a TABULAR format file, where the left column represents the names of categories
7 # and the other columns are headed by the names of criteria, such that each data value in the file
8 # represents the number of elements in a certain category satisfying a certain criterion.
9 # The output is a PDF file containing a stacked bars plot representing the number of elements in each
10 # category satisfying each criterion. The drawing is done using R code.
11
12
13 use strict;
14 use warnings;
15
16 my $criterion;
17 my @criteriaArray = ();
18 my $criteriaNumber = 0;
19 my $lineCounter = 0;
20
21 #variable to store the names of R script file
22 my $r_script;
23
24 # check to make sure having correct files
25 my $usage = "usage: draw_stacked_bar_plot.pl [TABULAR.in] [PDF.out] \n";
26 die $usage unless @ARGV == 2;
27
28 my $categoriesInputFile = $ARGV[0];
29
30 my $categories_criteria_bars_plot_outputFile = $ARGV[1];
31
32 #open the input file
33 open (INPUT, "<", $categoriesInputFile) || die("Could not open file $categoriesInputFile \n");
34 open (OUTPUT, ">", $categories_criteria_bars_plot_outputFile) || die("Could not open file $categories_criteria_bars_plot_outputFile \n");
35
36 # R script to implement the drawing of a stacked bar plot representing thes significant motifs in each category of motifs
37 #construct an R script file
38 $r_script = "motif_significance_bar_plot.r";
39 open(Rcmd,">", $r_script) or die "Cannot open $r_script \n\n";
40 print Rcmd "
41 #store the table content of the first file into a matrix
42 categoriesTable <- read.table(\"$categoriesInputFile\", header = TRUE);
43 categoriesMatrix <- as.matrix(categoriesTable);
44
45
46 #compute the sum of elements in the column with the maximum sum in each matrix
47 columnSumsVector <- colSums(categoriesMatrix);
48 maxColumn <- max (columnSumsVector);
49
50 if (maxColumn %% 10 != 0){
51 maxColumn <- maxColumn + 10;
52 }
53
54 plotHeight = maxColumn/8;
55 criteriaVector <- names(categoriesTable);
56
57 pdf(file = \"$categories_criteria_bars_plot_outputFile\", width = length(criteriaVector), height = plotHeight, family = \"Times\", pointsize = 12, onefile = TRUE);
58
59
60
61 #draw the first barplot
62 barplot(categoriesMatrix, ylab = \"No. of elements in each category\", xlab = \"Criteria\", ylim = range(0, maxColumn), col = \"black\", density = c(10, 20, 30, 40, 50, 60, 70, 80), angle = c(45, 90, 135), names.arg = criteriaVector);
63
64 #draw the legend
65 legendX = 0.2;
66 legendY = maxColumn;
67
68 legend (legendX, legendY, legend = rownames(categoriesMatrix), density = c(10, 20, 30, 40, 50, 60, 70, 80), angle = c(45, 90, 135));
69
70 dev.off();
71
72 #eof\n";
73 close Rcmd;
74 system("R --no-restore --no-save --no-readline < $r_script > $r_script.out");
75
76 #close the input files
77 close(OUTPUT);
78 close(INPUT);