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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tool_collections/galaxy_sequence_utils/fastq_filter commit a5766d27dcddd1891766476a913d0eae1ec7a3c9
author iuc
date Sun, 23 Nov 2025 17:50:05 +0000
parents 144275714590
children
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144275714590 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/galaxy_sequence_utils/fastq_filter commit bb5df9e62585e12f08dfc0a9f86eec8e205b4845
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1 <tool id="fastq_filter" name="Filter FASTQ" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
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2 <description>reads by quality score and length</description>
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81a9090d6df3 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/galaxy_sequence_utils/fastq_filter commit d4ced60a941c4c4a2fe95de9c09a10086810b387"
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3 <macros>
81a9090d6df3 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/galaxy_sequence_utils/fastq_filter commit d4ced60a941c4c4a2fe95de9c09a10086810b387"
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4 <import>macros.xml</import>
81a9090d6df3 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/galaxy_sequence_utils/fastq_filter commit d4ced60a941c4c4a2fe95de9c09a10086810b387"
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5 </macros>
3
10b75444feae "planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/galaxy_sequence_utils/fastq_filter commit 31edb920789fbd080260f853bc856be72fa7cfa8"
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6 <edam_topics>
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7 <edam_topic>topic_0622</edam_topic>
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8 </edam_topics>
10b75444feae "planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/galaxy_sequence_utils/fastq_filter commit 31edb920789fbd080260f853bc856be72fa7cfa8"
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9 <edam_operations>
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10 <edam_operation>operation_3695</edam_operation>
10b75444feae "planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/galaxy_sequence_utils/fastq_filter commit 31edb920789fbd080260f853bc856be72fa7cfa8"
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11 </edam_operations>
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12 <expand macro="requirements"/>
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13 <command><![CDATA[
06934412f56d planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/galaxy_sequence_utils/fastq_filter commit f2582539542b33240234e8ea6093e25d0aee9b6a
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14 gx-fastq-filter '$input_file' '$fastq_filter_file' '$output_file' '$output_file.files_path' '${input_file.extension[len( 'fastq' ):]}'
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15 ]]></command>
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16 <configfiles>
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17 <configfile name="fastq_filter_file"><![CDATA[
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18 def fastq_read_pass_filter(fastq_read):
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20 def mean(score_list):
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21 return float(sum(score_list)) / float(len(score_list))
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22
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23 if len(fastq_read) < $min_size:
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24 return False
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25 if $max_size > 0 and len(fastq_read) > $max_size:
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26 return False
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27 num_deviates = $max_num_deviants
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28 qual_scores = fastq_read.get_decimal_quality_scores()
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29 for qual_score in qual_scores:
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30 if qual_score < $min_quality or ($max_quality > 0 and qual_score > $max_quality):
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31 if num_deviates == 0:
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32 return False
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33 else:
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34 num_deviates -= 1
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35 #if not $paired_end:
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36 qual_scores_split = [qual_scores]
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37 #else:
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38 qual_scores_split = [qual_scores[0:int(len(qual_scores) / 2)], qual_scores[int(len(qual_scores) / 2): ]]
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39 #end if
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40 #for $fastq_filter in $fastq_filters:
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41 for split_scores in qual_scores_split:
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42 left_column_offset = $fastq_filter['offset_type']['left_column_offset']
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43 right_column_offset = $fastq_filter['offset_type']['right_column_offset']
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44 #if $fastq_filter['offset_type']['base_offset_type'] == 'offsets_percent':
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45 left_column_offset = int(round(float(left_column_offset) / 100.0 * float(len(split_scores))))
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46 right_column_offset = int(round(float(right_column_offset) / 100.0 * float(len(split_scores))))
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47 #end if
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48 if right_column_offset > 0:
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49 split_scores = split_scores[left_column_offset:-right_column_offset]
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50 else:
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51 split_scores = split_scores[left_column_offset:]
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52 if split_scores: ##if a read doesn't have enough columns, it passes by default
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53 if not (${fastq_filter['score_operation']}(split_scores) $fastq_filter['score_comparison'] $fastq_filter['score']):
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54 return False
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55 #end for
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56 return True
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57 ]]></configfile>
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58 </configfiles>
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59 <inputs>
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60 <param name="input_file" type="data" format="fastqsanger,fastqcssanger,fastqsanger.gz,fastqcssanger.gz,fastqsanger.bz2,fastqcssanger.bz2" label="FASTQ File" help="Requires groomed data: if your data does not appear here try using the FASTQ groomer"/>
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61 <param name="min_size" type="integer" min="0" value="0" label="Minimum size" />
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62 <param name="max_size" type="integer" value="0" label="Maximum size" help="A maximum size less than 1 indicates no limit"/>
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63 <param name="min_quality" type="float" value="0" label="Minimum quality"/>
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64 <param name="max_quality" type="float" value="0" label="Maximum quality" help="A maximum quality less than 1 indicates no limit"/>
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65 <param name="max_num_deviants" type="integer" min="0" value="0" label="Maximum number of bases allowed outside of quality range" />
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66 <param name="paired_end" type="boolean" truevalue="paired_end" falsevalue="single_end" checked="false" label="This is paired end data" />
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67 <repeat name="fastq_filters" title="Quality Filter on a Range of Bases" help="The above settings do not apply to these filters">
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68 <conditional name="offset_type">
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69 <param name="base_offset_type" type="select" label="Define Base Offsets as" help="Use Absolute for fixed length reads (Illumina, SOLiD)&lt;br&gt;Use Percentage for variable length reads (Roche/454)">
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70 <option value="offsets_absolute" selected="true">Absolute Values</option>
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71 <option value="offsets_percent">Percentage of Read Length</option>
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72 </param>
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73 <when value="offsets_absolute">
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74 <param name="left_column_offset" type="integer" min="0" value="0" label="Offset from 5' end" help="Values start at 0, increasing from the left" />
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75 <param name="right_column_offset" type="integer" min="0" value="0" label="Offset from 3' end" help="Values start at 0, increasing from the right" />
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76 </when>
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77 <when value="offsets_percent">
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78 <param name="left_column_offset" type="float" min="0" max="100" value="0" label="Offset from 5' end" />
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79 <param name="right_column_offset" type="float" min="0" max="100" value="0" label="Offset from 3' end" />
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80 </when>
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81 </conditional>
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82 <param name="score_operation" type="select" label="Aggregate read score for specified range">
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83 <option value="min" selected="true">min score</option>
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84 <option value="max">max score</option>
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85 <option value="sum">sum of scores</option>
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86 <option value="mean">mean of scores</option>
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87 </param>
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88 <param name="score_comparison" type="select" label="Keep read when aggregate score is">
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89 <option value="&gt;">&gt;</option>
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90 <option value="&gt;=" selected="true">&gt;=</option>
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91 <option value="==">==</option>
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92 <option value="&lt;">&lt;</option>
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93 <option value="&lt;=">&lt;=</option>
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94 <sanitizer sanitize="false"/>
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95 </param>
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96 <param name="score" type="float" value="0" label="Quality score" />
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97 </repeat>
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98 </inputs>
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99 <outputs>
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100 <data name="output_file" format_source="input_file" />
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101 </outputs>
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102 <tests>
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103 <!-- Do nothing filter -->
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104 <test>
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105 <param name="input_file" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger"/>
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106 <param name="min_size" value="0"/>
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107 <param name="max_size" value="0"/>
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108 <param name="min_quality" value="0"/>
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109 <param name="max_quality" value="0"/>
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110 <param name="max_num_deviants" value="0"/>
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111 <param name="paired_end" value="false"/>
6
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112 <repeat name="fastq_filters">
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113 <conditional name="offset_type">
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114 <param name="base_offset_type" value="offsets_absolute"/>
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115 <param name="left_column_offset" value="0"/>
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116 <param name="right_column_offset" value="0"/>
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117 </conditional>
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118 <param name="score_operation" value="min"/>
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119 <param name="score_comparison" value="&gt;="/>
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120 <param name="score" value="0"/>
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121 </repeat>
2
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122 <output name="output_file" file="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" />
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123 </test>
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124 <!-- Do nothing filter compressed -->
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125 <test>
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126 <param name="input_file" value="sanger_full_range_original_sanger.fastqsanger.gz" ftype="fastqsanger.gz"/>
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127 <param name="min_size" value="0"/>
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128 <param name="max_size" value="0"/>
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129 <param name="min_quality" value="0"/>
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130 <param name="max_quality" value="0"/>
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131 <param name="max_num_deviants" value="0"/>
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132 <param name="paired_end" value="false"/>
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133 <repeat name="fastq_filters">
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134 <conditional name="offset_type">
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135 <param name="base_offset_type" value="offsets_absolute"/>
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136 <param name="left_column_offset" value="0"/>
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137 <param name="right_column_offset" value="0"/>
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138 </conditional>
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139 <param name="score_operation" value="min"/>
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140 <param name="score_comparison" value="&gt;="/>
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141 <param name="score" value="0"/>
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142 </repeat>
2
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143 <output name="output_file" file="sanger_full_range_original_sanger.fastqsanger.gz" ftype="fastqsanger.gz" decompress="true"/>
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144 </test>
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145 <!-- crippled input types prevent this test <test>
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146 <param name="input_file" value="solexa_full_range_original_solexa.fastqsolexa" ftype="fastqsolexa"/>
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147 <param name="min_size" value="0"/>
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148 <param name="max_size" value="0"/>
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149 <param name="min_quality" value="-5"/>
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150 <param name="max_quality" value="0"/>
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151 <param name="max_num_deviants" value="0"/>
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152 <param name="paired_end" value="false"/>
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153 <param name="base_offset_type" value="offsets_absolute"/>
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154 <param name="left_column_offset" value="0"/>
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155 <param name="right_column_offset" value="0"/>
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156 <param name="score_operation" value="min"/>
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157 <param name="score_comparison" value="&gt;="/>
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158 <param name="score" value="-5"/>
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159 <output name="output_file" file="solexa_full_range_original_solexa.fastqsolexa" ftype="fastqsolexa"/>
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160 </test> -->
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161 <!-- No trim, so does not remove Adapter from cssanger -->
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162 <test>
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163 <param name="input_file" value="sanger_full_range_as_cssanger.fastqcssanger" ftype="fastqcssanger"/>
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164 <param name="min_size" value="0"/>
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165 <param name="max_size" value="0"/>
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166 <param name="min_quality" value="0"/>
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167 <param name="max_quality" value="0"/>
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168 <param name="max_num_deviants" value="0"/>
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169 <param name="paired_end" value="false"/>
6
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170 <repeat name="fastq_filters">
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171 <conditional name="offset_type">
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172 <param name="base_offset_type" value="offsets_absolute"/>
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173 <param name="left_column_offset" value="0"/>
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174 <param name="right_column_offset" value="0"/>
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175 </conditional>
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176 <param name="score_operation" value="min"/>
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177 <param name="score_comparison" value="&gt;="/>
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178 <param name="score" value="0"/>
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179 </repeat>
2
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180 <output name="output_file" file="sanger_full_range_as_cssanger.fastqcssanger" ftype="fastqcssanger" />
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181 </test>
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182 <!-- Remove all Filter -->
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183 <test>
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184 <param name="input_file" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger"/>
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185 <param name="min_size" value="0"/>
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186 <param name="max_size" value="0"/>
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187 <param name="min_quality" value="1"/>
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188 <param name="max_quality" value="0"/>
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189 <param name="max_num_deviants" value="0"/>
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190 <param name="paired_end" value="false"/>
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191 <repeat name="fastq_filters">
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192 <conditional name="offset_type">
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193 <param name="base_offset_type" value="offsets_absolute"/>
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194 <param name="left_column_offset" value="0"/>
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195 <param name="right_column_offset" value="0"/>
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196 </conditional>
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197 <param name="score_operation" value="min"/>
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198 <param name="score_comparison" value="&gt;="/>
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199 <param name="score" value="0"/>
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200 </repeat>
2
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201 <output name="output_file" file="empty_file.dat" ftype="fastqsanger" />
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202 </test>
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203 <!-- crippled input types prevent this test <test>
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204 <param name="input_file" value="solexa_full_range_original_solexa.fastqsolexa" ftype="fastqsolexa"/>
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205 <param name="min_size" value="0"/>
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206 <param name="max_size" value="0"/>
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207 <param name="min_quality" value="-4"/>
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208 <param name="max_quality" value="0"/>
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209 <param name="max_num_deviants" value="0"/>
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210 <param name="paired_end" value="false"/>
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211 <param name="base_offset_type" value="offsets_absolute"/>
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212 <param name="left_column_offset" value="0"/>
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213 <param name="right_column_offset" value="0"/>
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214 <param name="score_operation" value="min"/>
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215 <param name="score_comparison" value="&gt;="/>
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216 <param name="score" value="-5"/>
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217 <output name="output_file" file="empty_file.dat" ftype="fastqsolexa" />
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218 </test> -->
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219 <!-- Keep all by allowing 1 deviant -->
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220 <test>
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221 <param name="input_file" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger"/>
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222 <param name="min_size" value="0"/>
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223 <param name="max_size" value="0"/>
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224 <param name="min_quality" value="1"/>
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225 <param name="max_quality" value="0"/>
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226 <param name="max_num_deviants" value="1"/>
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227 <param name="paired_end" value="false"/>
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228 <repeat name="fastq_filters">
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229 <conditional name="offset_type">
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230 <param name="base_offset_type" value="offsets_absolute"/>
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231 <param name="left_column_offset" value="0"/>
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232 <param name="right_column_offset" value="0"/>
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233 </conditional>
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234 <param name="score_operation" value="min"/>
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235 <param name="score_comparison" value="&gt;="/>
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236 <param name="score" value="0"/>
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237 </repeat>
2
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238 <output name="output_file" file="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" />
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239 </test>
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240 <!-- crippled input types prevent this test<test>
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241 <param name="input_file" value="solexa_full_range_original_solexa.fastqsolexa" ftype="fastqsolexa"/>
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242 <param name="min_size" value="0"/>
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243 <param name="max_size" value="0"/>
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244 <param name="min_quality" value="-5"/>
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245 <param name="max_quality" value="61"/>
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246 <param name="max_num_deviants" value="1"/>
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247 <param name="paired_end" value="false"/>
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248 <param name="base_offset_type" value="offsets_absolute"/>
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249 <param name="left_column_offset" value="0"/>
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250 <param name="right_column_offset" value="0"/>
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251 <param name="score_operation" value="min"/>
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252 <param name="score_comparison" value="&gt;="/>
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253 <param name="score" value="-5"/>
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254 <output name="output_file" file="solexa_full_range_original_solexa.fastqsolexa" ftype="fastqsolexa" />
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255 </test> -->
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256 <!-- Filter inner range -->
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257 <test>
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258 <param name="input_file" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger"/>
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259 <param name="min_size" value="0"/>
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260 <param name="max_size" value="0"/>
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261 <param name="min_quality" value="0"/>
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262 <param name="max_quality" value="0"/>
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263 <param name="max_num_deviants" value="0"/>
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264 <param name="paired_end" value="false"/>
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265 <repeat name="fastq_filters">
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266 <conditional name="offset_type">
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267 <param name="base_offset_type" value="offsets_absolute"/>
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268 <param name="left_column_offset" value="1"/>
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269 <param name="right_column_offset" value="1"/>
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270 </conditional>
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271 <param name="score_operation" value="min"/>
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272 <param name="score_comparison" value="&gt;="/>
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273 <param name="score" value="1"/>
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274 </repeat>
3
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275 <output name="output_file" file="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" />
2
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276 </test>
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277 <test>
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278 <param name="input_file" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger"/>
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279 <param name="min_size" value="0"/>
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280 <param name="max_size" value="0"/>
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281 <param name="min_quality" value="0"/>
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282 <param name="max_quality" value="0"/>
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283 <param name="max_num_deviants" value="0"/>
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284 <param name="paired_end" value="false"/>
6
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285 <repeat name="fastq_filters">
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286 <conditional name="offset_type">
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287 <param name="base_offset_type" value="offsets_absolute"/>
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288 <param name="left_column_offset" value="1"/>
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289 <param name="right_column_offset" value="1"/>
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290 </conditional>
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291 <param name="score_operation" value="max"/>
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292 <param name="score_comparison" value="&lt;="/>
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293 <param name="score" value="92"/>
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294 </repeat>
2
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295 <output name="output_file" file="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" />
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296 </test>
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297 <!-- percent based offsets -->
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298 <test>
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299 <param name="input_file" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger"/>
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300 <param name="min_size" value="0"/>
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301 <param name="max_size" value="0"/>
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302 <param name="min_quality" value="0"/>
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303 <param name="max_quality" value="0"/>
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304 <param name="max_num_deviants" value="0"/>
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305 <param name="paired_end" value="false"/>
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306 <repeat name="fastq_filters">
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307 <conditional name="offset_type">
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308 <param name="base_offset_type" value="offsets_percent"/>
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309 <param name="left_column_offset" value="1.075"/>
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310 <param name="right_column_offset" value="1.075"/>
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311 </conditional>
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312 <param name="score_operation" value="min"/>
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313 <param name="score_comparison" value="&gt;="/>
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314 <param name="score" value="1"/>
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315 </repeat>
2
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316 <output name="output_file" file="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" />
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317 </test>
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318 <test>
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319 <param name="input_file" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger"/>
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320 <param name="min_size" value="0"/>
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321 <param name="max_size" value="0"/>
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322 <param name="min_quality" value="0"/>
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323 <param name="max_quality" value="0"/>
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324 <param name="max_num_deviants" value="0"/>
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325 <param name="paired_end" value="true"/>
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326 <repeat name="fastq_filters">
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327 <conditional name="offset_type">
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328 <param name="base_offset_type" value="offsets_percent"/>
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329 <param name="left_column_offset" value="1"/>
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330 <param name="right_column_offset" value="1"/>
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331 </conditional>
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332 <param name="score_operation" value="min"/>
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333 <param name="score_comparison" value="&gt;="/>
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334 <param name="score" value="1"/>
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335 </repeat>
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336 <output name="output_file" file="empty_file.dat" ftype="fastqsanger" />
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337 </test>
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338 </tests>
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339 <help><![CDATA[
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340 This tool allows you to build complex filters to be applied to each read in a FASTQ file.
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341
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342 **Basic Options:**
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343 * You can specify a minimum and maximum read lengths.
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344 * You can specify minimum and maximum per base quality scores, with optionally specifying the number of bases that are allowed to deviate from this range (default of 0 deviant bases).
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345 * If your data is paired-end, select the proper checkbox; this will cause each read to be internally split down the middle and filters applied to each half using the offsets specified.
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346
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347 **Advance Options:**
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348 * You can specify any number of advanced filters.
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349 * 5' and 3' offsets are defined, starting at zero, increasing from the respective end of the reads. For example, a quality string of "ABCDEFG", with 5' and 3' offsets of 1 and 1, respectively, specified will yield "BCDEF".
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350 * You can specify either absolute offset values, or percentage offset values. *Absolute Values* based offsets are useful for fixed length reads (e.g. Illumina or SOLiD data). *Percentage of Read Length* based offsets are useful for variable length reads (e.g. 454 data). When using the percent-based method, offsets are rounded to the nearest integer.
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351 * The user specifies the aggregating action (min, max, sum, mean) to perform on the quality score values found between the specified offsets to be used with the user defined comparison operation and comparison value.
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352 * If a set of offsets is specified that causes the remaining quality score list to be of length zero, then the read will **pass** the quality filter unless the size range filter is used to remove these reads.
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353
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354 -----
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355
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356 .. class:: warningmark
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357
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358 Adapter bases in color space reads are excluded from filtering.
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359 ]]></help>
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360 <citations>
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361 <citation type="doi">10.1093/bioinformatics/btq281</citation>
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362 </citations>
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363 </tool>