Mercurial > repos > devteam > fastq_paired_end_joiner
diff fastq_paired_end_joiner.xml @ 0:2793d1d765b9 draft
Imported from capsule None
author | devteam |
---|---|
date | Mon, 27 Jan 2014 09:25:44 -0500 |
parents | |
children | 270a8ed8a300 |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/fastq_paired_end_joiner.xml Mon Jan 27 09:25:44 2014 -0500 @@ -0,0 +1,65 @@ +<tool id="fastq_paired_end_joiner" name="FASTQ joiner" version="1.0.0"> + <description>on paired end reads</description> + <requirements> + <requirement type="package" version="1.0.0">galaxy_sequence_utils</requirement> + </requirements> + <command interpreter="python">fastq_paired_end_joiner.py '$input1_file' '${input1_file.extension[len( 'fastq' ):]}' '$input2_file' '${input2_file.extension[len( 'fastq' ):]}' '$output_file'</command> + <inputs> + <param name="input1_file" type="data" format="fastqsanger,fastqcssanger" label="Left-hand Reads" /> + <param name="input2_file" type="data" format="fastqsanger,fastqcssanger" label="Right-hand Reads" /> + </inputs> + <outputs> + <data name="output_file" format="input" /> + </outputs> + <tests> + <test> + <param name="input1_file" value="split_pair_reads_1.fastqsanger" ftype="fastqsanger" /> + <param name="input2_file" value="split_pair_reads_2.fastqsanger" ftype="fastqsanger" /> + <output name="output_file" file="3.fastqsanger" /> + </test> + </tests> + <help> +**What it does** + +This tool joins paired end FASTQ reads from two separate files into a single read in one file. The join is performed using sequence identifiers, allowing the two files to contain differing ordering. If a sequence identifier does not appear in both files, it is excluded from the output. + +Sequence identifiers with /1 and /2 appended override the left-hand and right-hand designation; i.e. if the reads end with /1 and /2, the read containing /1 will be used as the left-hand read and the read containing /2 will be used as the right-hand read. Sequences without this designation will follow the left-hand and right-hand settings set by the user. + +----- + +**Input formats** + +Left-hand Read:: + + @HWI-EAS91_1_30788AAXX:7:21:1542:1758/1 + GTCAATTGTACTGGTCAATACTAAAAGAATAGGATC + +HWI-EAS91_1_30788AAXX:7:21:1542:1758/1 + hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh + +Right-hand Read:: + + @HWI-EAS91_1_30788AAXX:7:21:1542:1758/2 + GCTCCTAGCATCTGGAGTCTCTATCACCTGAGCCCA + +HWI-EAS91_1_30788AAXX:7:21:1542:1758/2 + hhhhhhhhhhhhhhhhhhhhhhhh`hfhhVZSWehR + +----- + +**Output** + +A multiple-fastq file, for example:: + + @HWI-EAS91_1_30788AAXX:7:21:1542:1758 + GTCAATTGTACTGGTCAATACTAAAAGAATAGGATCGCTCCTAGCATCTGGAGTCTCTATCACCTGAGCCCA + +HWI-EAS91_1_30788AAXX:7:21:1542:1758 + hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh`hfhhVZSWehR + +------ + +**Citation** + +If you use this tool, please cite `Blankenberg D, Gordon A, Von Kuster G, Coraor N, Taylor J, Nekrutenko A; Galaxy Team. Manipulation of FASTQ data with Galaxy. Bioinformatics. 2010 Jul 15;26(14):1783-5. <http://www.ncbi.nlm.nih.gov/pubmed/20562416>`_ + + + </help> +</tool>