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1
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1 ##FastQC 0.11.2
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2 >>Basic Statistics pass
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3 #Measure Value
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4 Filename 1000gsample.fastq
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5 File type Conventional base calls
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6 Encoding Sanger / Illumina 1.9
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7 Total Sequences 5000
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8 Sequences flagged as poor quality 0
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9 Sequence length 54
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10 %GC 43
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11 >>END_MODULE
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12 >>Per base sequence quality warn
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13 #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile
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14 1 28.9946 31.0 27.0 33.0 22.0 34.0
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15 2 28.486 30.0 26.0 32.0 21.0 33.0
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16 3 28.5272 30.0 26.0 32.0 21.0 34.0
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17 4 28.6252 30.0 26.0 32.0 22.0 33.0
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|
18 5 28.4804 30.0 26.0 32.0 21.0 33.0
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|
19 6 28.5128 30.0 26.0 32.0 22.0 33.0
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|
20 7 28.4102 30.0 26.0 32.0 21.0 33.0
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|
21 8 28.4674 30.0 26.0 32.0 22.0 33.0
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|
22 9 28.182 30.0 26.0 32.0 21.0 33.0
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23 10 28.0908 30.0 26.0 32.0 21.0 33.0
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|
24 11 28.1876 30.0 26.0 32.0 21.0 33.0
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|
25 12 28.1742 30.0 26.0 32.0 21.0 33.0
|
|
|
26 13 27.923 30.0 25.0 32.0 20.0 33.0
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|
27 14 27.8984 29.0 25.0 32.0 21.0 33.0
|
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|
28 15 27.6112 29.0 25.0 32.0 20.0 33.0
|
|
|
29 16 27.6638 29.0 25.0 32.0 20.0 33.0
|
|
|
30 17 27.5734 29.0 25.0 32.0 20.0 33.0
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|
31 18 27.6348 29.0 25.0 32.0 20.0 33.0
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|
|
32 19 27.6526 29.0 25.0 32.0 20.0 33.0
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|
33 20 27.2716 29.0 24.0 32.0 19.0 33.0
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|
34 21 27.2642 29.0 25.0 31.0 19.0 33.0
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|
35 22 26.881 28.0 24.0 31.0 19.0 33.0
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|
36 23 26.8124 28.0 24.0 31.0 19.0 33.0
|
|
|
37 24 26.8204 28.0 24.0 31.0 19.0 33.0
|
|
|
38 25 26.6606 28.0 24.0 31.0 18.0 33.0
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|
39 26 26.3806 28.0 23.0 31.0 18.0 33.0
|
|
|
40 27 25.947 28.0 23.0 31.0 17.0 33.0
|
|
|
41 28 25.7446 27.0 22.0 31.0 16.0 33.0
|
|
|
42 29 25.6856 27.0 22.0 31.0 16.0 33.0
|
|
|
43 30 25.5228 27.0 22.0 31.0 16.0 33.0
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|
44 31 25.354 27.0 22.0 30.0 16.0 32.0
|
|
|
45 32 25.1656 27.0 22.0 30.0 16.0 32.0
|
|
|
46 33 25.0164 26.0 21.0 30.0 15.0 32.0
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|
47 34 24.75 26.0 21.0 30.0 15.0 32.0
|
|
|
48 35 24.3926 26.0 21.0 30.0 15.0 32.0
|
|
|
49 36 24.1122 25.0 20.0 30.0 14.0 32.0
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|
|
50 37 23.8612 25.0 20.0 29.0 13.0 32.0
|
|
|
51 38 23.7544 25.0 20.0 29.0 13.0 32.0
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|
|
52 39 23.485 25.0 19.0 29.0 13.0 32.0
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|
|
53 40 23.1984 24.0 19.0 29.0 13.0 32.0
|
|
|
54 41 23.0976 24.0 19.0 29.0 13.0 32.0
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|
|
55 42 22.9212 24.0 19.0 28.0 13.0 31.0
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|
|
56 43 22.5172 24.0 18.0 28.0 12.0 31.0
|
|
|
57 44 22.3234 23.0 18.0 28.0 12.0 31.0
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|
|
58 45 22.1372 23.0 18.0 28.0 11.0 31.0
|
|
|
59 46 22.2166 23.0 18.0 28.0 11.0 31.0
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|
|
60 47 22.075 23.0 18.0 28.0 11.0 31.0
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|
61 48 21.4592 22.0 17.0 27.0 11.0 31.0
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|
62 49 21.0954 22.0 16.0 27.0 10.0 30.0
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|
63 50 20.9356 22.0 16.0 27.0 10.0 30.0
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|
64 51 20.6152 21.0 15.0 27.0 9.0 30.0
|
|
|
65 52 20.6588 21.0 16.0 27.0 9.0 30.0
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66 53 19.5852 20.0 14.0 25.0 9.0 29.0
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67 54 19.9424 21.0 14.0 26.0 7.0 30.0
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68 >>END_MODULE
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69 >>Per tile sequence quality pass
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70 #Tile Base Mean
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|
71 1 1 0.4542485249902164
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|
72 1 2 0.02493171505959424
|
|
|
73 1 3 0.004493134032774293
|
|
|
74 1 4 0.6119404948560927
|
|
|
75 1 5 0.5283275008594686
|
|
|
76 1 6 0.3741933003058371
|
|
|
77 1 7 -0.2985554485298749
|
|
|
78 1 8 0.6040181642739206
|
|
|
79 1 9 0.43795745033596845
|
|
|
80 1 10 0.05327706110314523
|
|
|
81 1 11 0.8097461135057031
|
|
|
82 1 12 0.9171964750225641
|
|
|
83 1 13 1.0716973449965757
|
|
|
84 1 14 0.562649938864773
|
|
|
85 1 15 0.5112535943737946
|
|
|
86 1 16 0.7098461441939676
|
|
|
87 1 17 0.3989886413671577
|
|
|
88 1 18 0.2351551176870892
|
|
|
89 1 19 0.002716666680861124
|
|
|
90 1 20 -0.5270840838615776
|
|
|
91 1 21 0.08959510509382795
|
|
|
92 1 22 -0.6322238602545553
|
|
|
93 1 23 -0.8566085860715056
|
|
|
94 1 24 -0.8167218970288026
|
|
|
95 1 25 0.7728199138173544
|
|
|
96 1 26 -0.26898877195552373
|
|
|
97 1 27 -0.7734079986509634
|
|
|
98 1 28 0.46313879073469266
|
|
|
99 1 29 -1.0803714983510417
|
|
|
100 1 30 -0.9107347156963463
|
|
|
101 1 31 -1.1279249650605188
|
|
|
102 1 32 0.03588141895047414
|
|
|
103 1 33 -0.3276409211703317
|
|
|
104 1 34 0.07833890179158587
|
|
|
105 1 35 -0.8393131051954583
|
|
|
106 1 36 -1.024889600311596
|
|
|
107 1 37 -1.4177418858492992
|
|
|
108 1 38 -1.912918888443187
|
|
|
109 1 39 -1.880569234686881
|
|
|
110 1 40 -0.9901560786471286
|
|
|
111 1 41 -1.9499635516515283
|
|
|
112 1 42 -2.3754765893384793
|
|
|
113 1 43 -1.6682056704307335
|
|
|
114 1 44 -2.2432919652101226
|
|
|
115 1 45 -2.1214550638335794
|
|
|
116 1 46 -1.6941624871292404
|
|
|
117 1 47 -2.261740586778952
|
|
|
118 1 48 -0.47246064535067234
|
|
|
119 1 49 -1.345180096970381
|
|
|
120 1 50 -1.3012642855865373
|
|
|
121 1 51 -1.6748103331735038
|
|
|
122 1 52 -1.4936238782530324
|
|
|
123 1 53 -0.9900326328970834
|
|
|
124 1 54 -0.9042724595665739
|
|
|
125 2 1 0.27305194379363584
|
|
|
126 2 2 -0.40803531790744074
|
|
|
127 2 3 -1.054114924575284
|
|
|
128 2 4 0.02463890755450393
|
|
|
129 2 5 -1.0899875174555511
|
|
|
130 2 6 -0.7630472369346997
|
|
|
131 2 7 -1.2563088062832328
|
|
|
132 2 8 0.09022085047660511
|
|
|
133 2 9 -0.8821890698105506
|
|
|
134 2 10 -1.0053309975049132
|
|
|
135 2 11 -1.2879339841743942
|
|
|
136 2 12 -2.149225991399902
|
|
|
137 2 13 -1.2225639492647176
|
|
|
138 2 14 -0.7472401710253394
|
|
|
139 2 15 -0.9942409111207091
|
|
|
140 2 16 -1.07061783627001
|
|
|
141 2 17 -1.0825742401957257
|
|
|
142 2 18 -1.0554431729112004
|
|
|
143 2 19 -1.374084310120118
|
|
|
144 2 20 -0.25260300938050406
|
|
|
145 2 21 -0.7384879229891972
|
|
|
146 2 22 -0.5973032253339206
|
|
|
147 2 23 -1.926205655668575
|
|
|
148 2 24 -1.8018256821325878
|
|
|
149 2 25 -1.2574609164634758
|
|
|
150 2 26 -2.588402691369442
|
|
|
151 2 27 -1.909427534670499
|
|
|
152 2 28 -2.213051685455781
|
|
|
153 2 29 -3.343130961110507
|
|
|
154 2 30 -3.447731052692685
|
|
|
155 2 31 -3.261502498638052
|
|
|
156 2 32 -2.596108813039759
|
|
|
157 2 33 -2.173306366835778
|
|
|
158 2 34 -2.968059144606464
|
|
|
159 2 35 -3.110863776746129
|
|
|
160 2 36 -3.6207381961601897
|
|
|
161 2 37 -2.8287308968383122
|
|
|
162 2 38 -2.3431997187240157
|
|
|
163 2 39 -2.7157340698517167
|
|
|
164 2 40 -2.8763587648498152
|
|
|
165 2 41 -3.221758423446399
|
|
|
166 2 42 -3.735916149778042
|
|
|
167 2 43 -2.8279126301376927
|
|
|
168 2 44 -2.034012355930514
|
|
|
169 2 45 -3.745875088253605
|
|
|
170 2 46 -3.8848828778496305
|
|
|
171 2 47 -3.6639383889767565
|
|
|
172 2 48 -3.0197133926034176
|
|
|
173 2 49 -3.1119688637591487
|
|
|
174 2 50 -2.7486391329613866
|
|
|
175 2 51 -2.4279238862870542
|
|
|
176 2 52 -3.3607789454081
|
|
|
177 2 53 -4.239849482713934
|
|
|
178 2 54 -3.3794861347802474
|
|
|
179 3 1 0.4228103978999158
|
|
|
180 3 2 1.0474512245356244
|
|
|
181 3 3 0.4013716178677811
|
|
|
182 3 4 -0.7648710993468057
|
|
|
183 3 5 1.642931737202833
|
|
|
184 3 6 0.42190790454218075
|
|
|
185 3 7 0.5808202482371243
|
|
|
186 3 8 0.22341615758495692
|
|
|
187 3 9 0.06605109582092084
|
|
|
188 3 10 1.203778733343949
|
|
|
189 3 11 -0.3886931284117985
|
|
|
190 3 12 0.31385199341721304
|
|
|
191 3 13 0.7829570997345918
|
|
|
192 3 14 -0.2772608749590866
|
|
|
193 3 15 -0.2613216564623251
|
|
|
194 3 16 -0.6475674566978924
|
|
|
195 3 17 -1.0549689951991752
|
|
|
196 3 18 -1.3066509023798005
|
|
|
197 3 19 -0.22225546263909735
|
|
|
198 3 20 -1.028310393783542
|
|
|
199 3 21 -0.3112967566675984
|
|
|
200 3 22 -0.2177311066313692
|
|
|
201 3 23 1.415409251163723
|
|
|
202 3 24 1.0256415366389788
|
|
|
203 3 25 -0.5776817584234486
|
|
|
204 3 26 0.41573809538003914
|
|
|
205 3 27 1.098163907703551
|
|
|
206 3 28 0.5095156023288965
|
|
|
207 3 29 0.6313341872676865
|
|
|
208 3 30 0.4032006243259474
|
|
|
209 3 31 -0.21733410664357322
|
|
|
210 3 32 -0.1723683023427256
|
|
|
211 3 33 -0.8558460493754616
|
|
|
212 3 34 0.4094425807213504
|
|
|
213 3 35 1.0478663819840293
|
|
|
214 3 36 2.193616531238014
|
|
|
215 3 37 0.2458032646523698
|
|
|
216 3 38 0.5829562509102146
|
|
|
217 3 39 -0.23954359366124223
|
|
|
218 3 40 -0.6762207386248349
|
|
|
219 3 41 1.2420096924956283
|
|
|
220 3 42 1.0798188398699935
|
|
|
221 3 43 0.9132881980196572
|
|
|
222 3 44 0.7368641105981268
|
|
|
223 3 45 0.004297444527622929
|
|
|
224 3 46 1.1033848930268384
|
|
|
225 3 47 0.008939457814136631
|
|
|
226 3 48 -1.3137092518166682
|
|
|
227 3 49 -0.5681455373271262
|
|
|
228 3 50 1.1098839864312993
|
|
|
229 3 51 -0.835791381111072
|
|
|
230 3 52 -0.5478044802597211
|
|
|
231 3 53 -0.49036708105762017
|
|
|
232 3 54 0.06495830966419547
|
|
|
233 4 1 0.05082972157141441
|
|
|
234 4 2 0.528472618600496
|
|
|
235 4 3 -0.06998794044829992
|
|
|
236 4 4 0.9770198599354565
|
|
|
237 4 5 1.1004886730206387
|
|
|
238 4 6 0.1575876837002177
|
|
|
239 4 7 0.6801991302247039
|
|
|
240 4 8 0.5346652949210515
|
|
|
241 4 9 -0.2155224031438827
|
|
|
242 4 10 -0.29104528321919787
|
|
|
243 4 11 -0.7165054127458248
|
|
|
244 4 12 -1.561924404098317
|
|
|
245 4 13 -0.5241512508520181
|
|
|
246 4 14 -0.19168461546978222
|
|
|
247 4 15 -0.91487583175563
|
|
|
248 4 16 0.42144565579348026
|
|
|
249 4 17 0.7586956010741162
|
|
|
250 4 18 -0.26179237926040955
|
|
|
251 4 19 0.2449633089275025
|
|
|
252 4 20 0.5093017525242587
|
|
|
253 4 21 -0.3575355420368176
|
|
|
254 4 22 -1.5020651300958257
|
|
|
255 4 23 -0.19604692550984382
|
|
|
256 4 24 -0.008174888481793374
|
|
|
257 4 25 1.0917454327428722
|
|
|
258 4 26 0.7131846102178585
|
|
|
259 4 27 0.2493026240596592
|
|
|
260 4 28 0.9774245050204087
|
|
|
261 4 29 -0.45424207222161783
|
|
|
262 4 30 -1.4953501003117324
|
|
|
263 4 31 0.2146879775524262
|
|
|
264 4 32 0.9277007107697663
|
|
|
265 4 33 -0.26854446207387284
|
|
|
266 4 34 -0.30139247793979607
|
|
|
267 4 35 0.5240568581745073
|
|
|
268 4 36 -0.5572461326681264
|
|
|
269 4 37 0.3934913253839092
|
|
|
270 4 38 0.4345780590537629
|
|
|
271 4 39 1.0779167237990777
|
|
|
272 4 40 0.3299904414993904
|
|
|
273 4 41 0.39728919560121767
|
|
|
274 4 42 -0.5454399593018522
|
|
|
275 4 43 -2.6850554872805503
|
|
|
276 4 44 0.7913844694663119
|
|
|
277 4 45 0.8255534831749678
|
|
|
278 4 46 0.5436885507218001
|
|
|
279 4 47 -0.7591764842148514
|
|
|
280 4 48 0.5040961312061043
|
|
|
281 4 49 -0.5087942605845441
|
|
|
282 4 50 -1.3359407202629718
|
|
|
283 4 51 -0.2057016640648328
|
|
|
284 4 52 0.6868401022109474
|
|
|
285 4 53 0.26808702522257377
|
|
|
286 4 54 -0.23662899192310505
|
|
|
287 5 1 0.6850177557594499
|
|
|
288 5 2 0.48647017659805414
|
|
|
289 5 3 0.7275700571096984
|
|
|
290 5 4 0.38117126408686275
|
|
|
291 5 5 1.0514044239363898
|
|
|
292 5 6 1.066500992613527
|
|
|
293 5 7 0.9475983976239704
|
|
|
294 5 8 -0.05752029726454211
|
|
|
295 5 9 1.0994959118744312
|
|
|
296 5 10 0.6532770611031467
|
|
|
297 5 11 0.17897688273647105
|
|
|
298 5 12 0.1325810904071787
|
|
|
299 5 13 -0.05137957808034699
|
|
|
300 5 14 0.1318807080955402
|
|
|
301 5 15 -0.6425925594723587
|
|
|
302 5 16 0.15599999034781575
|
|
|
303 5 17 0.5220655644440804
|
|
|
304 5 18 0.9890012715332439
|
|
|
305 5 19 0.23348589745009107
|
|
|
306 5 20 0.10368514690765096
|
|
|
307 5 21 0.766518182016906
|
|
|
308 5 22 0.7523915243608279
|
|
|
309 5 23 -0.3643008937638115
|
|
|
310 5 24 0.2755857952788894
|
|
|
311 5 25 0.5112814522788938
|
|
|
312 5 26 -0.2997580027247544
|
|
|
313 5 27 -0.6964849217278868
|
|
|
314 5 28 0.6477541753500766
|
|
|
315 5 29 1.1350131170335729
|
|
|
316 5 30 0.6431114381498055
|
|
|
317 5 31 0.8413058041702506
|
|
|
318 5 32 -0.11796473489567916
|
|
|
319 5 33 -1.5430255365549463
|
|
|
320 5 34 0.0014158248685056662
|
|
|
321 5 35 -0.17777464365699558
|
|
|
322 5 36 0.29818732276532955
|
|
|
323 5 37 -0.1254341935416079
|
|
|
324 5 38 0.7332349577106605
|
|
|
325 5 39 0.011738457620811715
|
|
|
326 5 40 0.24061315212210133
|
|
|
327 5 41 -1.4576558593438378
|
|
|
328 5 42 0.10144648758459596
|
|
|
329 5 43 0.3933327911077278
|
|
|
330 5 44 -1.027907349825508
|
|
|
331 5 45 0.7708526284741133
|
|
|
332 5 46 -0.5557009486676989
|
|
|
333 5 47 0.2459517209133537
|
|
|
334 5 48 -0.9955375684275936
|
|
|
335 5 49 -0.11441086620115115
|
|
|
336 5 50 -0.37818736250961393
|
|
|
337 5 51 -0.22865648701965569
|
|
|
338 5 52 0.4756068909777369
|
|
|
339 5 53 0.2868904440259925
|
|
|
340 5 54 -1.0119647672588812
|
|
|
341 6 1 0.9498196205613141
|
|
|
342 6 2 -1.07903098890311
|
|
|
343 6 3 0.2142833438229843
|
|
|
344 6 4 -0.327453444537845
|
|
|
345 6 5 0.5204020929340594
|
|
|
346 6 6 -0.7269722008596666
|
|
|
347 6 7 -0.06871861869304396
|
|
|
348 6 8 -0.9992452389894844
|
|
|
349 6 9 -0.3410635286850088
|
|
|
350 6 10 0.020643028469113034
|
|
|
351 6 11 -0.022421718662130274
|
|
|
352 6 12 1.7367768946029827
|
|
|
353 6 13 -1.8257385524393221
|
|
|
354 6 14 0.8241884004032336
|
|
|
355 6 15 0.4646335477537491
|
|
|
356 6 16 0.47627971062753716
|
|
|
357 6 17 -0.18935634697783144
|
|
|
358 6 18 0.41930430183627365
|
|
|
359 6 19 1.0689171328813245
|
|
|
360 6 20 1.1297923730148796
|
|
|
361 6 21 0.6439074594061829
|
|
|
362 6 22 1.1011094730787754
|
|
|
363 6 23 -1.3461190755819956
|
|
|
364 6 24 -0.6041344844413885
|
|
|
365 6 25 0.27212061311805513
|
|
|
366 6 26 0.12588302291626974
|
|
|
367 6 27 1.011207385964422
|
|
|
368 6 28 -0.4035278759319745
|
|
|
369 6 29 1.075339457359913
|
|
|
370 6 30 2.019801414839783
|
|
|
371 6 31 0.45855522141966887
|
|
|
372 6 32 1.3375131205821766
|
|
|
373 6 33 3.2956691021396907
|
|
|
374 6 34 1.691392514845198
|
|
|
375 6 35 0.7448360789537283
|
|
|
376 6 36 1.7443411689191741
|
|
|
377 6 37 2.1193210512136353
|
|
|
378 6 38 1.125775750251453
|
|
|
379 6 39 -0.36075571487336333
|
|
|
380 6 40 0.7888649003738486
|
|
|
381 6 41 0.7479385462505697
|
|
|
382 6 42 -0.3246607385226312
|
|
|
383 6 43 1.5746847724597082
|
|
|
384 6 44 0.9068245849064276
|
|
|
385 6 45 1.3811090387305214
|
|
|
386 6 46 2.146863153896401
|
|
|
387 6 47 1.4529447279063632
|
|
|
388 6 48 0.023576650686624845
|
|
|
389 6 49 0.9601812083909245
|
|
|
390 6 50 -1.057441441763693
|
|
|
391 6 51 -0.19415765252082196
|
|
|
392 6 52 0.897518312889158
|
|
|
393 6 53 -1.4692867121511632
|
|
|
394 6 54 0.7619280066338909
|
|
|
395 7 1 -2.6562409854992914
|
|
|
396 7 2 -1.866909776781899
|
|
|
397 7 3 -0.24026211072246895
|
|
|
398 7 4 -0.20624132332572387
|
|
|
399 7 5 0.247674820206786
|
|
|
400 7 6 -0.4239418978293621
|
|
|
401 7 7 -0.765688315662743
|
|
|
402 7 8 -0.18106342080766424
|
|
|
403 7 9 0.8407546531331711
|
|
|
404 7 10 0.4448854527115387
|
|
|
405 7 11 -0.47696717320758353
|
|
|
406 7 12 -1.7177685599424706
|
|
|
407 7 13 -0.795435522136291
|
|
|
408 7 14 -0.7515691753543408
|
|
|
409 7 15 0.16160324472344456
|
|
|
410 7 16 -0.5843263499785252
|
|
|
411 7 17 0.5379163802948952
|
|
|
412 7 18 0.41930430183627365
|
|
|
413 7 19 -1.5068404428762499
|
|
|
414 7 20 -1.5065712633487571
|
|
|
415 7 21 0.674210489709214
|
|
|
416 7 22 0.9192912912605955
|
|
|
417 7 23 1.3811536516907346
|
|
|
418 7 24 0.9110170307101235
|
|
|
419 7 25 -1.4248490838516439
|
|
|
420 7 26 0.6410345380677853
|
|
|
421 7 27 -0.3221259473689102
|
|
|
422 7 28 -1.130800603204701
|
|
|
423 7 29 0.4692788512993076
|
|
|
424 7 30 -0.7680773730390058
|
|
|
425 7 31 -1.1778084149439678
|
|
|
426 7 32 -1.1473353642663078
|
|
|
427 7 33 -0.5225127160421259
|
|
|
428 7 34 -1.217698394245712
|
|
|
429 7 35 -0.43698210286445516
|
|
|
430 7 36 -0.8617194371414314
|
|
|
431 7 37 -0.8503759184833335
|
|
|
432 7 38 -1.4196787952030938
|
|
|
433 7 39 0.7301533760357302
|
|
|
434 7 40 0.7585618700708174
|
|
|
435 7 41 1.7479385462505697
|
|
|
436 7 42 1.0389756251137356
|
|
|
437 7 43 1.2716544694294072
|
|
|
438 7 44 0.8462185243003688
|
|
|
439 7 45 0.5023211599426425
|
|
|
440 7 46 -1.2167732097399622
|
|
|
441 7 47 0.9074901824518165
|
|
|
442 7 48 1.1750918022017771
|
|
|
443 7 49 0.56624181445153
|
|
|
444 7 50 0.6698312855090336
|
|
|
445 7 51 2.2300847717216037
|
|
|
446 7 52 0.4429728583437047
|
|
|
447 7 53 2.561016318151868
|
|
|
448 7 54 1.5498067945126799
|
|
|
449 8 1 -0.014529755552768364
|
|
|
450 8 2 0.3398638417564257
|
|
|
451 8 3 -0.884647137460437
|
|
|
452 8 4 -0.06453009337920079
|
|
|
453 8 5 -0.7006317751586337
|
|
|
454 8 6 -0.567435658970183
|
|
|
455 8 7 -0.3940305616520483
|
|
|
456 8 8 -0.3584252746401084
|
|
|
457 8 9 -0.09507422387217446
|
|
|
458 8 10 -1.100569092743008
|
|
|
459 8 11 -0.5250955154535717
|
|
|
460 8 12 0.009504167330256053
|
|
|
461 8 13 1.1938692906979327
|
|
|
462 8 14 1.000658988638527
|
|
|
463 8 15 0.6402128703918954
|
|
|
464 8 16 1.2944615288093537
|
|
|
465 8 17 -0.04942764822560264
|
|
|
466 8 18 1.183118918592065
|
|
|
467 8 19 0.842535671205745
|
|
|
468 8 20 0.47382089351398804
|
|
|
469 8 21 -0.23755421617313743
|
|
|
470 8 22 -0.6145768014310278
|
|
|
471 8 23 -0.7231244231755767
|
|
|
472 8 24 -0.6094820780242785
|
|
|
473 8 25 -0.5968633441011981
|
|
|
474 8 26 0.4984320425241151
|
|
|
475 8 27 0.27591326831736396
|
|
|
476 8 28 -0.3545082680888356
|
|
|
477 8 29 0.7847868726896827
|
|
|
478 8 30 1.3575910761588545
|
|
|
479 8 31 2.432708519102377
|
|
|
480 8 32 0.17886784428984015
|
|
|
481 8 33 1.3402323819970903
|
|
|
482 8 34 0.3045832456829878
|
|
|
483 8 35 -0.4031140101728319
|
|
|
484 8 36 0.3619882277427031
|
|
|
485 8 37 0.4401766661868969
|
|
|
486 8 38 1.317397853638262
|
|
|
487 8 39 0.5447701318289546
|
|
|
488 8 40 -0.6924185220860437
|
|
|
489 8 41 1.582163145181049
|
|
|
490 8 42 1.4489577998017893
|
|
|
491 8 43 0.8919753250443812
|
|
|
492 8 44 1.0173415189527724
|
|
|
493 8 45 1.1654227642207182
|
|
|
494 8 46 1.5194121735042465
|
|
|
495 8 47 2.5545490059812295
|
|
|
496 8 48 1.6777655989932185
|
|
|
497 8 49 0.3380778215816562
|
|
|
498 8 50 1.5263375243682127
|
|
|
499 8 51 -0.8929098806848152
|
|
|
500 8 52 1.233525443013935
|
|
|
501 8 53 0.5262569598630975
|
|
|
502 8 54 2.859075956723018
|
|
|
503 9 1 -1.2602813895396956
|
|
|
504 9 2 -0.5699400798122021
|
|
|
505 9 3 -0.4160196864800447
|
|
|
506 9 4 -0.2769483940327966
|
|
|
507 9 5 -1.8391938666619012
|
|
|
508 9 6 0.16393689004942402
|
|
|
509 9 7 0.004008654034226566
|
|
|
510 9 8 -0.611366451110694
|
|
|
511 9 9 -0.7996493872708683
|
|
|
512 9 10 -0.9450135371874531
|
|
|
513 9 11 1.1088914126510012
|
|
|
514 9 12 1.7428375006635903
|
|
|
515 9 13 0.1964836697829
|
|
|
516 9 14 -1.0424782662634335
|
|
|
517 9 15 0.3676638507840515
|
|
|
518 9 16 -1.349982915635092
|
|
|
519 9 17 0.45710829948681564
|
|
|
520 9 18 -0.5443320618000911
|
|
|
521 9 19 -0.16138589742170595
|
|
|
522 9 20 0.4045398477623543
|
|
|
523 9 21 -0.03690062140189809
|
|
|
524 9 22 0.6566650286343325
|
|
|
525 9 23 1.2801435506806307
|
|
|
526 9 24 1.0140473337404288
|
|
|
527 9 25 1.0155549565523962
|
|
|
528 9 26 -0.4963391993059503
|
|
|
529 9 27 -1.144348169591133
|
|
|
530 9 28 -0.29241676482086376
|
|
|
531 9 29 0.018773800794257056
|
|
|
532 9 30 0.5935387885771561
|
|
|
533 9 31 -0.04245487959043359
|
|
|
534 9 32 1.076907059976115
|
|
|
535 9 33 1.2108206172912048
|
|
|
536 9 34 0.16209958555226933
|
|
|
537 9 35 -0.3299113957937472
|
|
|
538 9 36 0.6554522800302856
|
|
|
539 9 37 0.16174529363787826
|
|
|
540 9 38 -0.29240606793036505
|
|
|
541 9 39 -0.46176581588346366
|
|
|
542 9 40 -0.17477146326251614
|
|
|
543 9 41 -0.3995362012241799
|
|
|
544 9 42 0.40059178672989404
|
|
|
545 9 43 -0.7929919952170579
|
|
|
546 9 44 -0.9578218797400382
|
|
|
547 9 45 0.6699979276194128
|
|
|
548 9 46 0.124640931674179
|
|
|
549 9 47 0.2408235157851486
|
|
|
550 9 48 -0.37844355133357865
|
|
|
551 9 49 0.3419993902091072
|
|
|
552 9 50 -0.8756232599455132
|
|
|
553 9 51 0.44826658990341883
|
|
|
554 9 52 0.34398295935380574
|
|
|
555 9 53 0.5347536918892395
|
|
|
556 9 54 -1.9350416903358045
|
|
|
557 10 1 1.0952741660158587
|
|
|
558 10 2 1.4967265868544644
|
|
|
559 10 3 1.317313646853286
|
|
|
560 10 4 -0.3547261718105723
|
|
|
561 10 5 -1.4614160888841212
|
|
|
562 10 6 0.29727022338276043
|
|
|
563 10 7 0.570675320700893
|
|
|
564 10 8 0.7553002155559696
|
|
|
565 10 9 -0.11076049838197832
|
|
|
566 10 10 0.9660975739236584
|
|
|
567 10 11 1.3200025237621134
|
|
|
568 10 12 0.5761708339969225
|
|
|
569 10 13 1.1742614475606779
|
|
|
570 10 14 0.49085506706990145
|
|
|
571 10 15 0.6676638507840522
|
|
|
572 10 16 0.5944615288093544
|
|
|
573 10 17 -0.2984472560687408
|
|
|
574 10 18 -0.07766539513342252
|
|
|
575 10 19 0.871947435911629
|
|
|
576 10 20 0.6934287366512422
|
|
|
577 10 21 -0.4924561769574538
|
|
|
578 10 22 0.13444280641211037
|
|
|
579 10 23 1.3356991062361878
|
|
|
580 10 24 0.6140473337404266
|
|
|
581 10 25 0.1933327343301734
|
|
|
582 10 26 1.2592163562496026
|
|
|
583 10 27 2.2112073859644212
|
|
|
584 10 28 1.7964721240680284
|
|
|
585 10 29 0.7632182452387006
|
|
|
586 10 30 1.604649899688269
|
|
|
587 10 31 1.8797673426317907
|
|
|
588 10 32 0.47690705997611715
|
|
|
589 10 33 -0.15584604937545876
|
|
|
590 10 34 1.8398773633300465
|
|
|
591 10 35 2.981199715317363
|
|
|
592 10 36 0.8110078355858406
|
|
|
593 10 37 1.8617452936378776
|
|
|
594 10 38 1.7742605987363014
|
|
|
595 10 39 3.2937897396720928
|
|
|
596 10 40 3.2918952034041524
|
|
|
597 10 41 1.3115749098869323
|
|
|
598 10 42 2.911702897841007
|
|
|
599 10 43 2.9292302270051636
|
|
|
600 10 44 1.964400342482186
|
|
|
601 10 45 0.5477757053971892
|
|
|
602 10 46 1.9135298205630704
|
|
|
603 10 47 1.2741568491184836
|
|
|
604 10 48 2.799334226444202
|
|
|
605 10 49 3.441999390209105
|
|
|
606 10 50 4.391043406721156
|
|
|
607 10 51 3.7815999232367545
|
|
|
608 10 52 1.3217607371315836
|
|
|
609 10 53 3.0125314696670173
|
|
|
610 10 54 2.2316249763308633
|
|
|
611 >>END_MODULE
|
|
|
612 >>Per sequence quality scores warn
|
|
|
613 #Quality Count
|
|
|
614 8 4.0
|
|
|
615 9 4.0
|
|
|
616 10 2.0
|
|
|
617 11 2.0
|
|
|
618 12 4.0
|
|
|
619 13 6.0
|
|
|
620 14 4.0
|
|
|
621 15 11.0
|
|
|
622 16 20.0
|
|
|
623 17 50.0
|
|
|
624 18 98.0
|
|
|
625 19 149.0
|
|
|
626 20 210.0
|
|
|
627 21 275.0
|
|
|
628 22 367.0
|
|
|
629 23 425.0
|
|
|
630 24 518.0
|
|
|
631 25 540.0
|
|
|
632 26 539.0
|
|
|
633 27 532.0
|
|
|
634 28 468.0
|
|
|
635 29 360.0
|
|
|
636 30 234.0
|
|
|
637 31 122.0
|
|
|
638 32 51.0
|
|
|
639 33 5.0
|
|
|
640 >>END_MODULE
|
|
|
641 >>Per base sequence content warn
|
|
|
642 #Base G A T C
|
|
|
643 1 21.5 32.64 25.919999999999998 19.939999999999998
|
|
|
644 2 24.04 31.72 26.540000000000003 17.7
|
|
|
645 3 21.58 28.860000000000003 29.42 20.14
|
|
|
646 4 21.58 28.28 28.9 21.240000000000002
|
|
|
647 5 21.16 29.720000000000002 28.32 20.8
|
|
|
648 6 22.38 29.24 25.72 22.66
|
|
|
649 7 21.34 30.320000000000004 28.24 20.1
|
|
|
650 8 21.58 29.060000000000002 27.939999999999998 21.42
|
|
|
651 9 20.3 28.48 28.58 22.64
|
|
|
652 10 21.2 30.84 26.56 21.4
|
|
|
653 11 21.14 28.64 27.279999999999998 22.939999999999998
|
|
|
654 12 21.32 28.88 28.999999999999996 20.8
|
|
|
655 13 20.36 29.220000000000002 28.939999999999998 21.48
|
|
|
656 14 20.02 29.2 29.220000000000002 21.560000000000002
|
|
|
657 15 20.200000000000003 29.220000000000002 28.38 22.2
|
|
|
658 16 20.34 29.38 29.299999999999997 20.979999999999997
|
|
|
659 17 20.599999999999998 29.14 28.499999999999996 21.759999999999998
|
|
|
660 18 20.599999999999998 27.92 29.4 22.08
|
|
|
661 19 21.66 28.46 28.58 21.3
|
|
|
662 20 20.0 29.24 28.96 21.8
|
|
|
663 21 21.72 28.499999999999996 28.18 21.6
|
|
|
664 22 20.52 30.880000000000003 27.3 21.3
|
|
|
665 23 20.72 28.9 29.720000000000002 20.66
|
|
|
666 24 21.12 28.62 27.58 22.68
|
|
|
667 25 21.099999999999998 29.060000000000002 29.160000000000004 20.68
|
|
|
668 26 22.18887555022009 28.391356542617046 28.131252501000397 21.288515406162464
|
|
|
669 27 21.344268853770753 28.525705141028208 28.765753150630125 21.364272854570913
|
|
|
670 28 19.34 30.54 27.46 22.66
|
|
|
671 29 20.90418083616723 29.12582516503301 29.045809161832363 20.924184836967395
|
|
|
672 30 21.099999999999998 29.4 27.439999999999998 22.06
|
|
|
673 31 20.84 28.04 28.660000000000004 22.46
|
|
|
674 32 19.847939175670266 28.371348539415763 28.7515006002401 23.02921168467387
|
|
|
675 33 22.084416883376676 28.62572514502901 27.265453090618124 22.024404880976196
|
|
|
676 34 21.304260852170433 29.285857171434287 27.265453090618124 22.144428885777156
|
|
|
677 35 21.084216843368676 29.425885177035404 28.225645129025807 21.264252850570113
|
|
|
678 36 21.404280856171233 28.665733146629325 28.805761152230446 21.124224844968996
|
|
|
679 37 20.888355342136855 29.43177270908363 28.53141256502601 21.1484593837535
|
|
|
680 38 21.864372874574915 29.02580516103221 28.065613122624526 21.044208841768352
|
|
|
681 39 21.928771508603443 28.251300520208083 27.050820328131252 22.769107643057225
|
|
|
682 40 21.32 28.499999999999996 27.82 22.36
|
|
|
683 41 21.673003802281368 28.116870122073244 27.61656994196518 22.59355613368021
|
|
|
684 42 22.29337602561537 28.036822093255953 27.71662997798679 21.953171903141886
|
|
|
685 43 21.244248849769953 28.425685137027408 27.825565113022606 22.504500900180034
|
|
|
686 44 21.404280856171233 28.985797159431886 27.545509101820365 22.064412882576516
|
|
|
687 45 21.86 29.28 26.740000000000002 22.12
|
|
|
688 46 21.26 27.54 27.74 23.46
|
|
|
689 47 21.628651460584233 28.271308523409367 28.051220488195277 22.048819527811126
|
|
|
690 48 21.279999999999998 28.439999999999998 26.400000000000002 23.880000000000003
|
|
|
691 49 21.044208841768352 29.045809161832363 27.185437087417487 22.724544908981798
|
|
|
692 50 21.82873149259704 28.15126050420168 26.810724289715886 23.209283713485394
|
|
|
693 51 21.028411364545818 27.73109243697479 28.011204481792717 23.229291716686674
|
|
|
694 52 21.824364872974595 30.146029205841167 25.94518903780756 22.084416883376676
|
|
|
695 53 21.164232846569313 28.325665133026607 28.045609121824366 22.464492898579717
|
|
|
696 54 21.53907976692787 27.024311834438418 26.90375728350412 24.5328511151296
|
|
|
697 >>END_MODULE
|
|
|
698 >>Per sequence GC content warn
|
|
|
699 #GC Content Count
|
|
|
700 0 3.0
|
|
|
701 1 5.0
|
|
|
702 2 7.0
|
|
|
703 3 7.0
|
|
|
704 4 7.0
|
|
|
705 5 5.0
|
|
|
706 6 2.0
|
|
|
707 7 1.0
|
|
|
708 8 1.5
|
|
|
709 9 2.0
|
|
|
710 10 1.5
|
|
|
711 11 1.0
|
|
|
712 12 2.5
|
|
|
713 13 4.0
|
|
|
714 14 4.0
|
|
|
715 15 4.0
|
|
|
716 16 5.0
|
|
|
717 17 6.0
|
|
|
718 18 11.0
|
|
|
719 19 19.5
|
|
|
720 20 23.0
|
|
|
721 21 37.5
|
|
|
722 22 52.0
|
|
|
723 23 61.0
|
|
|
724 24 70.0
|
|
|
725 25 79.0
|
|
|
726 26 88.0
|
|
|
727 27 117.5
|
|
|
728 28 147.0
|
|
|
729 29 159.5
|
|
|
730 30 172.0
|
|
|
731 31 203.0
|
|
|
732 32 243.0
|
|
|
733 33 252.0
|
|
|
734 34 290.0
|
|
|
735 35 328.0
|
|
|
736 36 330.0
|
|
|
737 37 332.0
|
|
|
738 38 332.5
|
|
|
739 39 333.0
|
|
|
740 40 342.0
|
|
|
741 41 351.0
|
|
|
742 42 343.5
|
|
|
743 43 336.0
|
|
|
744 44 318.5
|
|
|
745 45 279.5
|
|
|
746 46 258.0
|
|
|
747 47 254.5
|
|
|
748 48 251.0
|
|
|
749 49 234.5
|
|
|
750 50 218.0
|
|
|
751 51 209.5
|
|
|
752 52 201.0
|
|
|
753 53 198.5
|
|
|
754 54 196.0
|
|
|
755 55 177.0
|
|
|
756 56 162.5
|
|
|
757 57 167.0
|
|
|
758 58 147.0
|
|
|
759 59 127.0
|
|
|
760 60 106.5
|
|
|
761 61 86.0
|
|
|
762 62 85.0
|
|
|
763 63 84.0
|
|
|
764 64 61.5
|
|
|
765 65 39.0
|
|
|
766 66 42.5
|
|
|
767 67 46.0
|
|
|
768 68 34.0
|
|
|
769 69 17.0
|
|
|
770 70 12.0
|
|
|
771 71 14.0
|
|
|
772 72 16.0
|
|
|
773 73 12.5
|
|
|
774 74 9.0
|
|
|
775 75 6.5
|
|
|
776 76 4.0
|
|
|
777 77 4.5
|
|
|
778 78 5.0
|
|
|
779 79 5.0
|
|
|
780 80 5.0
|
|
|
781 81 3.0
|
|
|
782 82 1.0
|
|
|
783 83 1.0
|
|
|
784 84 1.0
|
|
|
785 85 1.0
|
|
|
786 86 1.0
|
|
|
787 87 1.0
|
|
|
788 88 1.0
|
|
|
789 89 1.0
|
|
|
790 90 2.0
|
|
|
791 91 3.0
|
|
|
792 92 2.5
|
|
|
793 93 2.0
|
|
|
794 94 2.0
|
|
|
795 95 3.0
|
|
|
796 96 4.0
|
|
|
797 97 5.0
|
|
|
798 98 6.0
|
|
|
799 99 3.5
|
|
|
800 100 1.0
|
|
|
801 >>END_MODULE
|
|
|
802 >>Per base N content pass
|
|
|
803 #Base N-Count
|
|
|
804 1 0.0
|
|
|
805 2 0.0
|
|
|
806 3 0.0
|
|
|
807 4 0.0
|
|
|
808 5 0.0
|
|
|
809 6 0.0
|
|
|
810 7 0.0
|
|
|
811 8 0.0
|
|
|
812 9 0.0
|
|
|
813 10 0.0
|
|
|
814 11 0.0
|
|
|
815 12 0.0
|
|
|
816 13 0.0
|
|
|
817 14 0.0
|
|
|
818 15 0.0
|
|
|
819 16 0.0
|
|
|
820 17 0.0
|
|
|
821 18 0.0
|
|
|
822 19 0.0
|
|
|
823 20 0.0
|
|
|
824 21 0.0
|
|
|
825 22 0.0
|
|
|
826 23 0.0
|
|
|
827 24 0.0
|
|
|
828 25 0.0
|
|
|
829 26 0.04
|
|
|
830 27 0.02
|
|
|
831 28 0.0
|
|
|
832 29 0.02
|
|
|
833 30 0.0
|
|
|
834 31 0.0
|
|
|
835 32 0.04
|
|
|
836 33 0.02
|
|
|
837 34 0.02
|
|
|
838 35 0.02
|
|
|
839 36 0.02
|
|
|
840 37 0.04
|
|
|
841 38 0.02
|
|
|
842 39 0.04
|
|
|
843 40 0.0
|
|
|
844 41 0.06
|
|
|
845 42 0.06
|
|
|
846 43 0.02
|
|
|
847 44 0.02
|
|
|
848 45 0.0
|
|
|
849 46 0.0
|
|
|
850 47 0.04
|
|
|
851 48 0.0
|
|
|
852 49 0.02
|
|
|
853 50 0.04
|
|
|
854 51 0.04
|
|
|
855 52 0.02
|
|
|
856 53 0.02
|
|
|
857 54 0.45999999999999996
|
|
|
858 >>END_MODULE
|
|
|
859 >>Sequence Length Distribution pass
|
|
|
860 #Length Count
|
|
|
861 54 5000.0
|
|
|
862 >>END_MODULE
|
|
|
863 >>Sequence Duplication Levels pass
|
|
|
864 #Total Deduplicated Percentage 99.52
|
|
|
865 #Duplication Level Percentage of deduplicated Percentage of total
|
|
|
866 1 99.81913183279742 99.33999999999999
|
|
|
867 2 0.08038585209003216 0.16
|
|
|
868 3 0.04019292604501608 0.12
|
|
|
869 4 0.0 0.0
|
|
|
870 5 0.02009646302250804 0.1
|
|
|
871 6 0.0 0.0
|
|
|
872 7 0.04019292604501608 0.27999999999999997
|
|
|
873 8 0.0 0.0
|
|
|
874 9 0.0 0.0
|
|
|
875 >10 0.0 0.0
|
|
|
876 >50 0.0 0.0
|
|
|
877 >100 0.0 0.0
|
|
|
878 >500 0.0 0.0
|
|
|
879 >1k 0.0 0.0
|
|
|
880 >5k 0.0 0.0
|
|
|
881 >10k+ 0.0 0.0
|
|
|
882 >>END_MODULE
|
|
|
883 >>Overrepresented sequences warn
|
|
|
884 #Sequence Count Percentage Possible Source
|
|
|
885 CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT 7 0.13999999999999999 Illumina Paired End PCR Primer 2 (96% over 33bp)
|
|
|
886 CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC 7 0.13999999999999999 Illumina Paired End PCR Primer 2 (100% over 35bp)
|
|
|
887 >>END_MODULE
|
|
|
888 >>Adapter Content pass
|
|
|
889 #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence
|
|
|
890 1 0.0 0.0 0.0
|
|
|
891 2 0.02 0.0 0.0
|
|
|
892 3 0.02 0.0 0.0
|
|
|
893 4 0.02 0.0 0.0
|
|
|
894 5 0.02 0.0 0.0
|
|
|
895 6 0.02 0.0 0.0
|
|
|
896 7 0.02 0.0 0.0
|
|
|
897 8 0.02 0.0 0.0
|
|
|
898 9 0.02 0.0 0.0
|
|
|
899 10 0.02 0.0 0.0
|
|
|
900 11 0.02 0.0 0.0
|
|
|
901 12 0.02 0.0 0.0
|
|
|
902 13 0.02 0.0 0.0
|
|
|
903 14 0.02 0.0 0.0
|
|
|
904 15 0.02 0.0 0.0
|
|
|
905 16 0.02 0.0 0.0
|
|
|
906 17 0.02 0.0 0.0
|
|
|
907 18 0.02 0.0 0.0
|
|
|
908 19 0.06 0.0 0.0
|
|
|
909 20 0.36 0.0 0.0
|
|
|
910 21 0.36 0.0 0.0
|
|
|
911 22 0.36 0.0 0.0
|
|
|
912 23 0.36 0.0 0.0
|
|
|
913 24 0.36 0.0 0.0
|
|
|
914 25 0.4 0.0 0.0
|
|
|
915 26 0.4 0.0 0.0
|
|
|
916 27 0.42 0.0 0.0
|
|
|
917 28 0.62 0.0 0.0
|
|
|
918 29 0.66 0.0 0.0
|
|
|
919 30 0.68 0.0 0.0
|
|
|
920 31 0.68 0.0 0.0
|
|
|
921 32 0.68 0.0 0.0
|
|
|
922 33 0.68 0.0 0.0
|
|
|
923 34 0.7 0.0 0.0
|
|
|
924 35 0.76 0.0 0.0
|
|
|
925 36 0.8 0.0 0.0
|
|
|
926 37 0.88 0.0 0.0
|
|
|
927 38 1.0 0.0 0.0
|
|
|
928 39 1.12 0.0 0.0
|
|
|
929 40 1.22 0.0 0.0
|
|
|
930 41 1.38 0.0 0.0
|
|
|
931 42 1.48 0.0 0.0
|
|
|
932 >>END_MODULE
|
|
|
933 >>Kmer Content pass
|
|
|
934 >>END_MODULE
|
