|
0
|
1 # This file contains a list of potential contaminants which are
|
|
|
2 # frequently found in high throughput sequencing reactions. These
|
|
|
3 # are mostly sequences of adapters / primers used in the various
|
|
|
4 # sequencing chemistries.
|
|
|
5 #
|
|
|
6 # Please DO NOT rely on these sequences to design your own oligos, some
|
|
|
7 # of them are truncated at ambiguous positions, and none of them are
|
|
|
8 # definitive sequences from the manufacturers so don't blame us if you
|
|
|
9 # try to use them and they don't work.
|
|
|
10 #
|
|
|
11 # You can add more sequences to the file by putting one line per entry
|
|
|
12 # and specifying a name[tab]sequence. If the contaminant you add is
|
|
|
13 # likely to be of use to others please consider sending it to the FastQ
|
|
|
14 # authors, either via a bug report at www.bioinformatics.bbsrc.ac.uk/bugzilla/
|
|
|
15 # or by directly emailing simon.andrews@bbsrc.ac.uk so other users of
|
|
|
16 # the program can benefit.
|
|
|
17
|
|
|
18 Illumina Single End Apapter 1 ACACTCTTTCCCTACACGACGCTGTTCCATCT
|
|
|
19 Illumina Single End Apapter 2 CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT
|
|
|
20 Illumina Single End PCR Primer 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
|
|
|
21 Illumina Single End PCR Primer 2 CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT
|
|
|
22 Illumina Single End Sequencing Primer ACACTCTTTCCCTACACGACGCTCTTCCGATCT
|
|
|
23
|
|
|
24 Illumina Paired End Adapter 1 ACACTCTTTCCCTACACGACGCTCTTCCGATCT
|
|
|
25 Illumina Paired End Adapter 2 CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT
|
|
|
26 Illumina Paried End PCR Primer 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
|
|
|
27 Illumina Paired End PCR Primer 2 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT
|
|
|
28 Illumina Paried End Sequencing Primer 1 ACACTCTTTCCCTACACGACGCTCTTCCGATCT
|
|
|
29 Illumina Paired End Sequencing Primer 2 CGGTCTCGGCATTCCTACTGAACCGCTCTTCCGATCT
|
|
|
30
|
|
|
31 Illumina DpnII expression Adapter 1 ACAGGTTCAGAGTTCTACAGTCCGAC
|
|
|
32 Illumina DpnII expression Adapter 2 CAAGCAGAAGACGGCATACGA
|
|
|
33 Illumina DpnII expression PCR Primer 1 CAAGCAGAAGACGGCATACGA
|
|
|
34 Illumina DpnII expression PCR Primer 2 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
|
|
|
35 Illumina DpnII expression Sequencing Primer CGACAGGTTCAGAGTTCTACAGTCCGACGATC
|
|
|
36
|
|
|
37 Illumina NlaIII expression Adapter 1 ACAGGTTCAGAGTTCTACAGTCCGACATG
|
|
|
38 Illumina NlaIII expression Adapter 2 CAAGCAGAAGACGGCATACGA
|
|
|
39 Illumina NlaIII expression PCR Primer 1 CAAGCAGAAGACGGCATACGA
|
|
|
40 Illumina NlaIII expression PCR Primer 2 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
|
|
|
41 Illumina NlaIII expression Sequencing Primer CCGACAGGTTCAGAGTTCTACAGTCCGACATG
|
|
|
42
|
|
|
43 Illumina Small RNA Adapter 1 GTTCAGAGTTCTACAGTCCGACGATC
|
|
|
44 Illumina Small RNA Adapter 2 TCGTATGCCGTCTTCTGCTTGT
|
|
|
45 Illumina Small RNA RT Primer CAAGCAGAAGACGGCATACGA
|
|
|
46 Illumina Small RNA PCR Primer 1 CAAGCAGAAGACGGCATACGA
|
|
|
47 Illumina Small RNA PCR Primer 2 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
|
|
|
48 Illumina Small RNA Sequencing Primer CGACAGGTTCAGAGTTCTACAGTCCGACGATC
|
|
|
49
|
|
|
50 Illumina Multiplexing Adapter 1 GATCGGAAGAGCACACGTCT
|
|
|
51 Illumina Multiplexing Adapter 2 ACACTCTTTCCCTACACGACGCTCTTCCGATCT
|
|
|
52 Illumina Multiplexing PCR Primer 1.01 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
|
|
|
53 Illumina Multiplexing PCR Primer 2.01 GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
|
|
|
54 Illumina Multiplexing Read1 Sequencing Primer ACACTCTTTCCCTACACGACGCTCTTCCGATCT
|
|
|
55 Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC
|
|
|
56 Illumina Multiplexing Read2 Sequencing Primer GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
|
|
|
57
|
|
|
58 Illumina PCR Primer Index 1 CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTC
|
|
|
59 Illumina PCR Primer Index 2 CAAGCAGAAGACGGCATACGAGATACATCGGTGACTGGAGTTC
|
|
|
60 Illumina PCR Primer Index 3 CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTC
|
|
|
61 Illumina PCR Primer Index 4 CAAGCAGAAGACGGCATACGAGATTGGTCAGTGACTGGAGTTC
|
|
|
62 Illumina PCR Primer Index 5 CAAGCAGAAGACGGCATACGAGATCACTGTGTGACTGGAGTTC
|
|
|
63 Illumina PCR Primer Index 6 CAAGCAGAAGACGGCATACGAGATATTGGCGTGACTGGAGTTC
|
|
|
64 Illumina PCR Primer Index 7 CAAGCAGAAGACGGCATACGAGATGATCTGGTGACTGGAGTTC
|
|
|
65 Illumina PCR Primer Index 8 CAAGCAGAAGACGGCATACGAGATTCAAGTGTGACTGGAGTTC
|
|
|
66 Illumina PCR Primer Index 9 CAAGCAGAAGACGGCATACGAGATCTGATCGTGACTGGAGTTC
|
|
|
67 Illumina PCR Primer Index 10 CAAGCAGAAGACGGCATACGAGATAAGCTAGTGACTGGAGTTC
|
|
|
68 Illumina PCR Primer Index 11 CAAGCAGAAGACGGCATACGAGATGTAGCCGTGACTGGAGTTC
|
|
|
69 Illumina PCR Primer Index 12 CAAGCAGAAGACGGCATACGAGATTACAAGGTGACTGGAGTTC
|
|
|
70
|
|
|
71 Illumina DpnII Gex Adapter 1 GATCGTCGGACTGTAGAACTCTGAAC
|
|
|
72 Illumina DpnII Gex Adapter 1.01 ACAGGTTCAGAGTTCTACAGTCCGAC
|
|
|
73 Illumina DpnII Gex Adapter 2 CAAGCAGAAGACGGCATACGA
|
|
|
74 Illumina DpnII Gex Adapter 2.01 TCGTATGCCGTCTTCTGCTTG
|
|
|
75 Illumina DpnII Gex PCR Primer 1 CAAGCAGAAGACGGCATACGA
|
|
|
76 Illumina DpnII Gex PCR Primer 2 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
|
|
|
77 Illumina DpnII Gex Sequencing Primer CGACAGGTTCAGAGTTCTACAGTCCGACGATC
|
|
|
78
|
|
|
79 Illumina NlaIII Gex Adapter 1.01 TCGGACTGTAGAACTCTGAAC
|
|
|
80 Illumina NlaIII Gex Adapter 1.02 ACAGGTTCAGAGTTCTACAGTCCGACATG
|
|
|
81 Illumina NlaIII Gex Adapter 2.01 CAAGCAGAAGACGGCATACGA
|
|
|
82 Illumina NlaIII Gex Adapter 2.02 TCGTATGCCGTCTTCTGCTTG
|
|
|
83 Illumina NlaIII Gex PCR Primer 1 CAAGCAGAAGACGGCATACGA
|
|
|
84 Illumina NlaIII Gex PCR Primer 2 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
|
|
|
85 Illumina NlaIII Gex Sequencing Primer CCGACAGGTTCAGAGTTCTACAGTCCGACATG
|
|
|
86
|
|
|
87 Illumina Small RNA RT Primer CAAGCAGAAGACGGCATACGA
|
|
|
88 Illumina 5p RNA Adapter GTTCAGAGTTCTACAGTCCGACGATC
|
|
|
89 Illumina RNA Adapter1 TCGTATGCCGTCTTCTGCTTGT
|
|
|
90
|
|
|
91 Illumina Small RNA 3p Adapter 1 ATCTCGTATGCCGTCTTCTGCTTG
|
|
|
92 Illumina Small RNA PCR Primer 1 CAAGCAGAAGACGGCATACGA
|
|
|
93 Illumina Small RNA PCR Primer 2 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
|
|
|
94 Illumina Small RNA Sequencing Primer CGACAGGTTCAGAGTTCTACAGTCCGACGATC
|
|
|
95
|
|
|
96 TruSeq Universal Adapter AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
|
|
|
97 TruSeq Adapter, Index 1 GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG
|
|
|
98 TruSeq Adapter, Index 2 GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTG
|
|
|
99 TruSeq Adapter, Index 3 GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTG
|
|
|
100 TruSeq Adapter, Index 4 GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTG
|
|
|
101 TruSeq Adapter, Index 5 GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTG
|
|
|
102 TruSeq Adapter, Index 6 GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTG
|
|
|
103 TruSeq Adapter, Index 7 GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTG
|
|
|
104 TruSeq Adapter, Index 8 GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTG
|
|
|
105 TruSeq Adapter, Index 9 GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTG
|
|
|
106 TruSeq Adapter, Index 10 GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTG
|
|
|
107 TruSeq Adapter, Index 11 GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTG
|
|
|
108 TruSeq Adapter, Index 12 GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTG
|
|
|
109
|
|
|
110 Illumina RNA RT Primer GCCTTGGCACCCGAGAATTCCA
|
|
|
111 Illumina RNA PCR Primer AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGA
|
|
|
112
|
|
|
113 RNA PCR Primer, Index 1 CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
114 RNA PCR Primer, Index 2 CAAGCAGAAGACGGCATACGAGATACATCGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
115 RNA PCR Primer, Index 3 CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
116 RNA PCR Primer, Index 4 CAAGCAGAAGACGGCATACGAGATTGGTCAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
117 RNA PCR Primer, Index 5 CAAGCAGAAGACGGCATACGAGATCACTGTGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
118 RNA PCR Primer, Index 6 CAAGCAGAAGACGGCATACGAGATATTGGCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
119 RNA PCR Primer, Index 7 CAAGCAGAAGACGGCATACGAGATGATCTGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
120 RNA PCR Primer, Index 8 CAAGCAGAAGACGGCATACGAGATTCAAGTGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
121 RNA PCR Primer, Index 9 CAAGCAGAAGACGGCATACGAGATCTGATCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
122 RNA PCR Primer, Index 10 CAAGCAGAAGACGGCATACGAGATAAGCTAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
123 RNA PCR Primer, Index 11 CAAGCAGAAGACGGCATACGAGATGTAGCCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
124 RNA PCR Primer, Index 12 CAAGCAGAAGACGGCATACGAGATTACAAGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
125 RNA PCR Primer, Index 13 CAAGCAGAAGACGGCATACGAGATTTGACTGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
126 RNA PCR Primer, Index 14 CAAGCAGAAGACGGCATACGAGATGGAACTGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
127 RNA PCR Primer, Index 15 CAAGCAGAAGACGGCATACGAGATTGACATGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
128 RNA PCR Primer, Index 16 CAAGCAGAAGACGGCATACGAGATGGACGGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
129 RNA PCR Primer, Index 17 CAAGCAGAAGACGGCATACGAGATCTCTACGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
130 RNA PCR Primer, Index 18 CAAGCAGAAGACGGCATACGAGATGCGGACGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
131 RNA PCR Primer, Index 19 CAAGCAGAAGACGGCATACGAGATTTTCACGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
132 RNA PCR Primer, Index 20 CAAGCAGAAGACGGCATACGAGATGGCCACGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
133 RNA PCR Primer, Index 21 CAAGCAGAAGACGGCATACGAGATCGAAACGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
134 RNA PCR Primer, Index 22 CAAGCAGAAGACGGCATACGAGATCGTACGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
135 RNA PCR Primer, Index 23 CAAGCAGAAGACGGCATACGAGATCCACTCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
136 RNA PCR Primer, Index 24 CAAGCAGAAGACGGCATACGAGATGCTACCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
137 RNA PCR Primer, Index 25 CAAGCAGAAGACGGCATACGAGATATCAGTGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
138 RNA PCR Primer, Index 26 CAAGCAGAAGACGGCATACGAGATGCTCATGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
139 RNA PCR Primer, Index 27 CAAGCAGAAGACGGCATACGAGATAGGAATGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
140 RNA PCR Primer, Index 28 CAAGCAGAAGACGGCATACGAGATCTTTTGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
141 RNA PCR Primer, Index 29 CAAGCAGAAGACGGCATACGAGATTAGTTGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
142 RNA PCR Primer, Index 30 CAAGCAGAAGACGGCATACGAGATCCGGTGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
143 RNA PCR Primer, Index 31 CAAGCAGAAGACGGCATACGAGATATCGTGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
144 RNA PCR Primer, Index 32 CAAGCAGAAGACGGCATACGAGATTGAGTGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
145 RNA PCR Primer, Index 33 CAAGCAGAAGACGGCATACGAGATCGCCTGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
146 RNA PCR Primer, Index 34 CAAGCAGAAGACGGCATACGAGATGCCATGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
147 RNA PCR Primer, Index 35 CAAGCAGAAGACGGCATACGAGATAAAATGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
148 RNA PCR Primer, Index 36 CAAGCAGAAGACGGCATACGAGATTGTTGGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
149 RNA PCR Primer, Index 37 CAAGCAGAAGACGGCATACGAGATATTCCGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
150 RNA PCR Primer, Index 38 CAAGCAGAAGACGGCATACGAGATAGCTAGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
151 RNA PCR Primer, Index 39 CAAGCAGAAGACGGCATACGAGATGTATAGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
152 RNA PCR Primer, Index 40 CAAGCAGAAGACGGCATACGAGATTCTGAGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
153 RNA PCR Primer, Index 41 CAAGCAGAAGACGGCATACGAGATGTCGTCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
154 RNA PCR Primer, Index 42 CAAGCAGAAGACGGCATACGAGATCGATTAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
155 RNA PCR Primer, Index 43 CAAGCAGAAGACGGCATACGAGATGCTGTAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
156 RNA PCR Primer, Index 44 CAAGCAGAAGACGGCATACGAGATATTATAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
157 RNA PCR Primer, Index 45 CAAGCAGAAGACGGCATACGAGATGAATGAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
158 RNA PCR Primer, Index 46 CAAGCAGAAGACGGCATACGAGATTCGGGAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
159 RNA PCR Primer, Index 47 CAAGCAGAAGACGGCATACGAGATCTTCGAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
160 RNA PCR Primer, Index 48 CAAGCAGAAGACGGCATACGAGATTGCCGAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
|
|
|
161
|
|
|
162 ABI Dynabead EcoP Oligo CTGATCTAGAGGTACCGGATCCCAGCAGT
|
|
|
163 ABI Solid3 Adapter A CTGCCCCGGGTTCCTCATTCTCTCAGCAGCATG
|
|
|
164 ABI Solid3 Adapter B CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGAT
|
|
|
165 ABI Solid3 5' AMP Primer CCACTACGCCTCCGCTTTCCTCTCTATG
|
|
|
166 ABI Solid3 3' AMP Primer CTGCCCCGGGTTCCTCATTCT
|
|
|
167 ABI Solid3 EF1 alpha Sense Primer CATGTGTGTTGAGAGCTTC
|
|
|
168 ABI Solid3 EF1 alpha Antisense Primer GAAAACCAAAGTGGTCCAC
|
|
|
169 ABI Solid3 GAPDH Forward Primer TTAGCACCCCTGGCCAAGG
|
|
|
170 ABI Solid3 GAPDH Reverse Primer CTTACTCCTTGGAGGCCATG
|
