comparison test-data/fastqc_data.txt @ 18:3e1cdf5406db draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastqc commit 8a5d4f0470a7778a87d01dc84578f94a30ab7f43
author iuc
date Mon, 05 Nov 2018 12:08:29 -0500
parents ff9530579d1f
children 9da02be9c6cc
comparison
equal deleted inserted replaced
17:c15237684a01 18:3e1cdf5406db
1 ##FastQC 0.11.6 1 ##FastQC 0.11.8
2 >>Basic Statistics pass 2 >>Basic Statistics pass
3 #Measure Value 3 #Measure Value
4 Filename 1000gsample_fastq_bz2.bz2 4 Filename 1000trimmed_fastq
5 File type Conventional base calls 5 File type Conventional base calls
6 Encoding Sanger / Illumina 1.9 6 Encoding Sanger / Illumina 1.9
7 Total Sequences 5000 7 Total Sequences 4905
8 Sequences flagged as poor quality 0 8 Sequences flagged as poor quality 0
9 Sequence length 54 9 Sequence length 1-108
10 %GC 43 10 %GC 40
11 >>END_MODULE 11 >>END_MODULE
12 >>Per base sequence quality warn 12 >>Per base sequence quality pass
13 #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 13 #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile
14 1 28.9946 31.0 27.0 33.0 22.0 34.0 14 1 29.282772680937818 31.0 27.0 33.0 23.0 34.0
15 2 28.486 30.0 26.0 32.0 21.0 33.0 15 2 28.832925336597306 30.0 27.0 32.0 22.0 33.0
16 3 28.5272 30.0 26.0 32.0 21.0 34.0 16 3 28.896953588223266 30.0 27.0 32.0 23.0 34.0
17 4 28.6252 30.0 26.0 32.0 22.0 33.0 17 4 29.006785934608267 30.0 27.0 32.0 23.0 34.0
18 5 28.4804 30.0 26.0 32.0 21.0 33.0 18 5 28.9377990430622 30.0 27.0 32.0 22.0 34.0
19 6 28.5128 30.0 26.0 32.0 22.0 33.0 19 6 29.002938706968934 30.0 27.0 32.0 23.0 34.0
20 7 28.4102 30.0 26.0 32.0 21.0 33.0 20 7 28.899830220713074 30.0 27.0 32.0 22.0 34.0
21 8 28.4674 30.0 26.0 32.0 22.0 33.0 21 8 28.900706184463942 30.0 27.0 32.0 22.0 33.0
22 9 28.182 30.0 26.0 32.0 21.0 33.0 22 9 28.754602555772145 30.0 27.0 32.0 22.0 34.0
23 10 28.0908 30.0 26.0 32.0 21.0 33.0 23 10-11 28.822083022456294 30.0 27.0 32.0 22.5 33.0
24 11 28.1876 30.0 26.0 32.0 21.0 33.0 24 12-13 28.74898578710789 30.0 26.5 32.0 22.0 33.0
25 12 28.1742 30.0 26.0 32.0 21.0 33.0 25 14-15 28.53370750727077 30.0 26.0 32.0 22.0 33.5
26 13 27.923 30.0 25.0 32.0 20.0 33.0 26 16-17 28.52661127583153 30.0 26.0 32.0 22.0 33.0
27 14 27.8984 29.0 25.0 32.0 21.0 33.0 27 18-19 28.68343192244741 30.0 26.0 32.0 22.5 33.0
28 15 27.6112 29.0 25.0 32.0 20.0 33.0 28 20-21 28.370130166336985 29.5 26.0 32.0 22.0 33.0
29 16 27.6638 29.0 25.0 32.0 20.0 33.0 29 22-23 28.185946266098476 29.0 26.0 32.0 22.0 33.0
30 17 27.5734 29.0 25.0 32.0 20.0 33.0 30 24-25 28.13009469533431 29.0 26.0 32.0 21.5 33.0
31 18 27.6348 29.0 25.0 32.0 20.0 33.0 31 26-27 28.075768267577 29.0 26.0 32.0 21.5 33.0
32 19 27.6526 29.0 25.0 32.0 20.0 33.0 32 28-29 27.828024188665616 29.0 25.0 32.0 21.0 33.0
33 20 27.2716 29.0 24.0 32.0 19.0 33.0 33 30-31 27.792826876513317 29.0 25.0 31.5 21.0 33.0
34 21 27.2642 29.0 25.0 31.0 19.0 33.0 34 32-33 27.490989073616355 28.0 25.0 31.0 21.0 33.0
35 22 26.881 28.0 24.0 31.0 19.0 33.0 35 34-35 27.357199679261562 28.0 24.5 31.0 21.0 33.0
36 23 26.8124 28.0 24.0 31.0 19.0 33.0 36 36-37 27.062158129410527 28.0 24.0 31.0 20.5 33.0
37 24 26.8204 28.0 24.0 31.0 19.0 33.0 37 38-39 27.238022216783758 28.0 24.0 31.0 21.0 33.0
38 25 26.6606 28.0 24.0 31.0 18.0 33.0 38 40-41 27.028963434226593 28.0 24.0 31.0 20.5 33.0
39 26 26.3806 28.0 23.0 31.0 18.0 33.0 39 42-43 26.90862078496675 27.0 24.0 31.0 20.5 33.0
40 27 25.947 28.0 23.0 31.0 17.0 33.0 40 44-45 26.632003369751054 27.0 24.0 30.0 20.0 32.0
41 28 25.7446 27.0 22.0 31.0 16.0 33.0 41 46-47 26.83042786373109 27.5 24.0 31.0 20.5 32.0
42 29 25.6856 27.0 22.0 31.0 16.0 33.0 42 48-49 26.431681832255798 27.0 23.5 30.0 20.0 32.0
43 30 25.5228 27.0 22.0 31.0 16.0 33.0 43 50-51 26.219000735104444 27.0 23.0 30.0 20.0 32.0
44 31 25.354 27.0 22.0 30.0 16.0 32.0 44 52-53 25.925690423864786 26.5 22.5 29.5 19.5 31.5
45 32 25.1656 27.0 22.0 30.0 16.0 32.0 45 54-55 27.995241016389798 29.5 25.5 31.5 20.5 33.0
46 33 25.0164 26.0 21.0 30.0 15.0 32.0 46 56-57 30.410857897242785 31.5 28.5 33.0 25.5 34.0
47 34 24.75 26.0 21.0 30.0 15.0 32.0 47 58-59 30.4083885209713 31.5 28.5 33.0 25.5 34.0
48 35 24.3926 26.0 21.0 30.0 15.0 32.0 48 60-61 30.444812362030905 31.5 29.0 33.0 26.0 34.0
49 36 24.1122 25.0 20.0 30.0 14.0 32.0 49 62-63 30.44039735099338 31.5 29.0 33.0 25.5 34.0
50 37 23.8612 25.0 20.0 29.0 13.0 32.0 50 64-65 30.441712697003883 31.5 29.0 33.0 25.5 34.0
51 38 23.7544 25.0 20.0 29.0 13.0 32.0 51 66-67 30.51141384388807 32.0 29.0 33.0 25.5 34.0
52 39 23.485 25.0 19.0 29.0 13.0 32.0 52 68-69 30.05117820324006 31.0 28.0 33.0 24.5 34.0
53 40 23.1984 24.0 19.0 29.0 13.0 32.0 53 70-71 30.276141384388808 31.0 29.0 33.0 25.0 34.0
54 41 23.0976 24.0 19.0 29.0 13.0 32.0 54 72-73 30.385493372606774 31.5 29.0 33.0 25.5 34.0
55 42 22.9212 24.0 19.0 28.0 13.0 31.0 55 74-75 30.213549337260677 31.0 28.5 33.0 25.5 34.0
56 43 22.5172 24.0 18.0 28.0 12.0 31.0 56 76-77 29.678939617083948 31.0 28.0 33.0 24.5 34.0
57 44 22.3234 23.0 18.0 28.0 12.0 31.0 57 78-79 30.019145802650957 31.0 28.0 33.0 24.5 34.0
58 45 22.1372 23.0 18.0 28.0 11.0 31.0 58 80-81 29.664212076583212 31.0 27.5 33.0 24.0 34.0
59 46 22.2166 23.0 18.0 28.0 11.0 31.0 59 82-83 29.653534609720175 31.0 28.0 32.5 24.5 34.0
60 47 22.075 23.0 18.0 28.0 11.0 31.0 60 84-85 29.425625920471283 30.5 27.0 32.0 24.0 34.0
61 48 21.4592 22.0 17.0 27.0 11.0 31.0 61 86-87 29.34057437407953 30.0 27.0 32.5 24.0 34.0
62 49 21.0954 22.0 16.0 27.0 10.0 30.0 62 88-89 28.785714285714285 30.0 27.0 32.0 22.5 33.5
63 50 20.9356 22.0 16.0 27.0 10.0 30.0 63 90-91 28.667525773195877 29.5 26.0 32.0 23.0 33.0
64 51 20.6152 21.0 15.0 27.0 9.0 30.0 64 92-93 28.565905743740796 29.0 26.0 32.0 23.0 33.0
65 52 20.6588 21.0 16.0 27.0 9.0 30.0 65 94-95 28.271723122238583 29.0 26.0 32.0 22.5 33.0
66 53 19.5852 20.0 14.0 25.0 9.0 29.0 66 96-97 27.94955817378498 29.0 25.5 31.0 22.0 33.0
67 54 19.9424 21.0 14.0 26.0 7.0 30.0 67 98-99 27.641132075471695 28.0 25.0 31.0 21.5 33.0
68 >>END_MODULE 68 100-101 27.614716981132077 28.0 25.0 31.0 21.5 33.0
69 >>Per tile sequence quality pass 69 102-103 27.196226415094337 28.0 24.5 31.0 21.0 32.5
70 #Tile Base Mean 70 104-105 26.870943396226416 27.5 24.0 31.0 20.0 32.5
71 1 1 0.4542485249902164 71 106-107 26.367593937711003 27.0 23.5 30.0 20.5 32.0
72 1 2 0.02493171505959424 72 108 27.5650623885918 28.0 24.0 31.0 22.0 33.0
73 1 3 0.004493134032774293
74 1 4 0.6119404948560927
75 1 5 0.5283275008594686
76 1 6 0.3741933003058371
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79 1 9 0.43795745033596845
80 1 10 0.05327706110314523
81 1 11 0.8097461135057031
82 1 12 0.9171964750225641
83 1 13 1.0716973449965757
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85 1 15 0.5112535943737946
86 1 16 0.7098461441939676
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89 1 19 0.002716666680861124
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154 2 30 -3.447731052692685
155 2 31 -3.261502498638052
156 2 32 -2.596108813039759
157 2 33 -2.173306366835778
158 2 34 -2.968059144606464
159 2 35 -3.110863776746129
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179 3 1 0.4228103978999158
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184 3 6 0.42190790454218075
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186 3 8 0.22341615758495692
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269 4 37 0.3934913253839092
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271 4 39 1.0779167237990777
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288 5 2 0.48647017659805414
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292 5 6 1.066500992613527
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371 6 31 0.45855522141966887
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414 7 20 -1.5065712633487571
415 7 21 0.674210489709214
416 7 22 0.9192912912605955
417 7 23 1.3811536516907346
418 7 24 0.9110170307101235
419 7 25 -1.4248490838516439
420 7 26 0.6410345380677853
421 7 27 -0.3221259473689102
422 7 28 -1.130800603204701
423 7 29 0.4692788512993076
424 7 30 -0.7680773730390058
425 7 31 -1.1778084149439678
426 7 32 -1.1473353642663078
427 7 33 -0.5225127160421259
428 7 34 -1.217698394245712
429 7 35 -0.43698210286445516
430 7 36 -0.8617194371414314
431 7 37 -0.8503759184833335
432 7 38 -1.4196787952030938
433 7 39 0.7301533760357302
434 7 40 0.7585618700708174
435 7 41 1.7479385462505697
436 7 42 1.0389756251137356
437 7 43 1.2716544694294072
438 7 44 0.8462185243003688
439 7 45 0.5023211599426425
440 7 46 -1.2167732097399622
441 7 47 0.9074901824518165
442 7 48 1.1750918022017771
443 7 49 0.56624181445153
444 7 50 0.6698312855090336
445 7 51 2.2300847717216037
446 7 52 0.4429728583437047
447 7 53 2.561016318151868
448 7 54 1.5498067945126799
449 8 1 -0.014529755552768364
450 8 2 0.3398638417564257
451 8 3 -0.884647137460437
452 8 4 -0.06453009337920079
453 8 5 -0.7006317751586337
454 8 6 -0.567435658970183
455 8 7 -0.3940305616520483
456 8 8 -0.3584252746401084
457 8 9 -0.09507422387217446
458 8 10 -1.100569092743008
459 8 11 -0.5250955154535717
460 8 12 0.009504167330256053
461 8 13 1.1938692906979327
462 8 14 1.000658988638527
463 8 15 0.6402128703918954
464 8 16 1.2944615288093537
465 8 17 -0.04942764822560264
466 8 18 1.183118918592065
467 8 19 0.842535671205745
468 8 20 0.47382089351398804
469 8 21 -0.23755421617313743
470 8 22 -0.6145768014310278
471 8 23 -0.7231244231755767
472 8 24 -0.6094820780242785
473 8 25 -0.5968633441011981
474 8 26 0.4984320425241151
475 8 27 0.27591326831736396
476 8 28 -0.3545082680888356
477 8 29 0.7847868726896827
478 8 30 1.3575910761588545
479 8 31 2.432708519102377
480 8 32 0.17886784428984015
481 8 33 1.3402323819970903
482 8 34 0.3045832456829878
483 8 35 -0.4031140101728319
484 8 36 0.3619882277427031
485 8 37 0.4401766661868969
486 8 38 1.317397853638262
487 8 39 0.5447701318289546
488 8 40 -0.6924185220860437
489 8 41 1.582163145181049
490 8 42 1.4489577998017893
491 8 43 0.8919753250443812
492 8 44 1.0173415189527724
493 8 45 1.1654227642207182
494 8 46 1.5194121735042465
495 8 47 2.5545490059812295
496 8 48 1.6777655989932185
497 8 49 0.3380778215816562
498 8 50 1.5263375243682127
499 8 51 -0.8929098806848152
500 8 52 1.233525443013935
501 8 53 0.5262569598630975
502 8 54 2.859075956723018
503 9 1 -1.2602813895396956
504 9 2 -0.5699400798122021
505 9 3 -0.4160196864800447
506 9 4 -0.2769483940327966
507 9 5 -1.8391938666619012
508 9 6 0.16393689004942402
509 9 7 0.004008654034226566
510 9 8 -0.611366451110694
511 9 9 -0.7996493872708683
512 9 10 -0.9450135371874531
513 9 11 1.1088914126510012
514 9 12 1.7428375006635903
515 9 13 0.1964836697829
516 9 14 -1.0424782662634335
517 9 15 0.3676638507840515
518 9 16 -1.349982915635092
519 9 17 0.45710829948681564
520 9 18 -0.5443320618000911
521 9 19 -0.16138589742170595
522 9 20 0.4045398477623543
523 9 21 -0.03690062140189809
524 9 22 0.6566650286343325
525 9 23 1.2801435506806307
526 9 24 1.0140473337404288
527 9 25 1.0155549565523962
528 9 26 -0.4963391993059503
529 9 27 -1.144348169591133
530 9 28 -0.29241676482086376
531 9 29 0.018773800794257056
532 9 30 0.5935387885771561
533 9 31 -0.04245487959043359
534 9 32 1.076907059976115
535 9 33 1.2108206172912048
536 9 34 0.16209958555226933
537 9 35 -0.3299113957937472
538 9 36 0.6554522800302856
539 9 37 0.16174529363787826
540 9 38 -0.29240606793036505
541 9 39 -0.46176581588346366
542 9 40 -0.17477146326251614
543 9 41 -0.3995362012241799
544 9 42 0.40059178672989404
545 9 43 -0.7929919952170579
546 9 44 -0.9578218797400382
547 9 45 0.6699979276194128
548 9 46 0.124640931674179
549 9 47 0.2408235157851486
550 9 48 -0.37844355133357865
551 9 49 0.3419993902091072
552 9 50 -0.8756232599455132
553 9 51 0.44826658990341883
554 9 52 0.34398295935380574
555 9 53 0.5347536918892395
556 9 54 -1.9350416903358045
557 10 1 1.0952741660158587
558 10 2 1.4967265868544644
559 10 3 1.317313646853286
560 10 4 -0.3547261718105723
561 10 5 -1.4614160888841212
562 10 6 0.29727022338276043
563 10 7 0.570675320700893
564 10 8 0.7553002155559696
565 10 9 -0.11076049838197832
566 10 10 0.9660975739236584
567 10 11 1.3200025237621134
568 10 12 0.5761708339969225
569 10 13 1.1742614475606779
570 10 14 0.49085506706990145
571 10 15 0.6676638507840522
572 10 16 0.5944615288093544
573 10 17 -0.2984472560687408
574 10 18 -0.07766539513342252
575 10 19 0.871947435911629
576 10 20 0.6934287366512422
577 10 21 -0.4924561769574538
578 10 22 0.13444280641211037
579 10 23 1.3356991062361878
580 10 24 0.6140473337404266
581 10 25 0.1933327343301734
582 10 26 1.2592163562496026
583 10 27 2.2112073859644212
584 10 28 1.7964721240680284
585 10 29 0.7632182452387006
586 10 30 1.604649899688269
587 10 31 1.8797673426317907
588 10 32 0.47690705997611715
589 10 33 -0.15584604937545876
590 10 34 1.8398773633300465
591 10 35 2.981199715317363
592 10 36 0.8110078355858406
593 10 37 1.8617452936378776
594 10 38 1.7742605987363014
595 10 39 3.2937897396720928
596 10 40 3.2918952034041524
597 10 41 1.3115749098869323
598 10 42 2.911702897841007
599 10 43 2.9292302270051636
600 10 44 1.964400342482186
601 10 45 0.5477757053971892
602 10 46 1.9135298205630704
603 10 47 1.2741568491184836
604 10 48 2.799334226444202
605 10 49 3.441999390209105
606 10 50 4.391043406721156
607 10 51 3.7815999232367545
608 10 52 1.3217607371315836
609 10 53 3.0125314696670173
610 10 54 2.2316249763308633
611 >>END_MODULE 73 >>END_MODULE
612 >>Per sequence quality scores warn 74 >>Per sequence quality scores warn
613 #Quality Count 75 #Quality Count
614 8 4.0 76 20 7.0
615 9 4.0 77 21 24.0
616 10 2.0 78 22 47.0
617 11 2.0 79 23 78.0
618 12 4.0 80 24 226.0
619 13 6.0 81 25 513.0
620 14 4.0 82 26 830.0
621 15 11.0 83 27 1017.0
622 16 20.0 84 28 947.0
623 17 50.0 85 29 645.0
624 18 98.0 86 30 352.0
625 19 149.0 87 31 157.0
626 20 210.0 88 32 55.0
627 21 275.0 89 33 6.0
628 22 367.0 90 34 1.0
629 23 425.0
630 24 518.0
631 25 540.0
632 26 539.0
633 27 532.0
634 28 468.0
635 29 360.0
636 30 234.0
637 31 122.0
638 32 51.0
639 33 5.0
640 >>END_MODULE 91 >>END_MODULE
641 >>Per base sequence content pass 92 >>Per base sequence content pass
642 #Base G A T C 93 #Base G A T C
643 1 21.5 32.64 25.919999999999998 19.939999999999998 94 1 21.223241590214066 33.00713557594291 25.97349643221203 19.79612640163099
644 2 24.04 31.72 26.540000000000003 17.7 95 2 23.76580987352101 31.415748674010608 27.315381476948186 17.503059975520195
645 3 21.58 28.860000000000003 29.42 20.14 96 3 21.876916785933346 28.70578613780413 29.46227765283173 19.95501942343079
646 4 21.58 28.28 28.9 21.240000000000002 97 4 21.55048324079786 28.295290972650626 28.850503804236066 21.303721982315444
647 5 21.16 29.720000000000002 28.32 20.8 98 5 21.219055543998337 30.205949656750576 28.271271063033076 20.303723736218014
648 6 22.38 29.24 25.72 22.66 99 6 22.124265323257767 28.946263643996637 26.364399664147776 22.565071368597817
649 7 21.34 30.320000000000004 28.24 20.1 100 7 21.943972835314092 29.9660441426146 28.395585738539896 19.69439728353141
650 8 21.58 29.060000000000002 27.939999999999998 21.42 101 8 21.378129681147016 28.825165846351382 27.8621870318853 21.934517440616304
651 9 20.3 28.48 28.58 22.64 102 9 20.077972709551656 29.088152479965345 28.654970760233915 22.17890405024908
652 10 21.2 30.84 26.56 21.4 103 10-11 21.243237275035884 29.468919068124105 27.680247322512976 21.607596334327038
653 11 21.14 28.64 27.279999999999998 22.939999999999998 104 12-13 20.8276950834461 29.03698691926026 29.18358141632837 20.951736580965267
654 12 21.32 28.88 28.999999999999996 20.8 105 14-15 20.24001846295869 28.975305792753286 29.471497807523654 21.313177936764365
655 13 20.36 29.220000000000002 28.939999999999998 21.48 106 16-17 19.83872880351002 29.396418830783826 29.621724178821296 21.14312818688486
656 14 20.02 29.2 29.220000000000002 21.560000000000002 107 18-19 20.845552297165202 27.871456500488755 29.973118279569892 21.309872922776147
657 15 20.200000000000003 29.220000000000002 28.38 22.2 108 20-21 20.538172715894866 28.973717146433042 29.111389236545683 21.376720901126408
658 16 20.34 29.38 29.299999999999997 20.979999999999997 109 22-23 20.88082901554404 30.129533678756477 29.300518134715027 19.689119170984455
659 17 20.599999999999998 29.14 28.499999999999996 21.759999999999998 110 24-25 20.506974248927037 29.07725321888412 29.05042918454936 21.365343347639485
660 18 20.599999999999998 27.92 29.4 22.08 111 26-27 20.86603139013453 28.054932735426007 30.339125560538115 20.739910313901344
661 19 21.66 28.46 28.58 21.3 112 28-29 19.32600732600733 30.842490842490843 28.776556776556777 21.054945054945055
662 20 20.0 29.24 28.96 21.8 113 30-31 20.638820638820636 29.19226044226044 29.49938574938575 20.669533169533167
663 21 21.72 28.499999999999996 28.18 21.6 114 32-33 19.47989016314004 29.009853012437407 29.38136003876595 22.1288967856566
664 22 20.52 30.880000000000003 27.3 21.3 115 34-35 19.6674020023757 29.713219073477003 30.205328355676226 20.41405056847107
665 23 20.72 28.9 29.720000000000002 20.66 116 36-37 20.443482963764197 29.853975121687398 30.61114115738237 19.091400757166035
666 24 21.12 28.62 27.58 22.68 117 38-39 20.992661259173424 28.041714947856317 30.088837388953266 20.876786404016993
667 25 21.099999999999998 29.060000000000002 29.160000000000004 20.68 118 40-41 19.860197368421055 28.680098684210524 30.32483552631579 21.134868421052634
668 26 22.18887555022009 28.391356542617046 28.131252501000397 21.288515406162464 119 42-43 20.01321003963012 27.873183619550858 30.823425803610743 21.29018053720828
669 27 21.344268853770753 28.525705141028208 28.765753150630125 21.364272854570913 120 44-45 18.941371943982908 30.192262046047947 29.954901495371466 20.911464514597675
670 28 19.34 30.54 27.46 22.66 121 46-47 20.1888718734048 28.126595201633485 31.470137825421133 20.21439509954058
671 29 20.90418083616723 29.12582516503301 29.045809161832363 20.924184836967395 122 48-49 19.56159822419534 29.689234184239734 28.77358490566038 21.975582685904552
672 30 21.099999999999998 29.4 27.439999999999998 22.06 123 50-51 20.50656087885261 28.2575526396094 30.759841318278912 20.47604516325908
673 31 20.84 28.04 28.660000000000004 22.46 124 52-53 17.691256830601095 29.918032786885245 31.864754098360653 20.525956284153004
674 32 19.847939175670266 28.371348539415763 28.7515006002401 23.02921168467387 125 54-55 17.214912280701753 32.30994152046784 29.53216374269006 20.942982456140353
675 33 22.084416883376676 28.62572514502901 27.265453090618124 22.024404880976196 126 56-57 19.30856932695844 30.893710923133504 30.893710923133504 18.90400882677455
676 34 21.304260852170433 29.285857171434287 27.265453090618124 22.144428885777156 127 58-59 20.014716703458426 29.72774098601913 31.42016188373804 18.8373804267844
677 35 21.084216843368676 29.425885177035404 28.225645129025807 21.264252850570113 128 60-61 17.402501839587934 33.18616629874908 29.506990434142754 19.904341427520237
678 36 21.404280856171233 28.665733146629325 28.805761152230446 21.124224844968996 129 62-63 18.543046357615893 29.72774098601913 32.2663723325975 19.462840323767477
679 37 20.888355342136855 29.43177270908363 28.53141256502601 21.1484593837535 130 64-65 16.893632683106365 32.94074346705926 30.180345969819655 19.985277880014724
680 38 21.864372874574915 29.02580516103221 28.065613122624526 21.044208841768352 131 66-67 18.519882179675996 28.902798232695137 32.80559646539028 19.771723122238587
681 39 21.928771508603443 28.251300520208083 27.050820328131252 22.769107643057225 132 68-69 17.2680412371134 30.375552282768776 31.66421207658321 20.692194403534607
682 40 21.32 28.499999999999996 27.82 22.36 133 70-71 17.673048600883654 27.798232695139912 35.34609720176731 19.182621502209134
683 41 21.673003802281368 28.116870122073244 27.61656994196518 22.59355613368021 134 72-73 16.200294550810014 29.528718703976438 33.87334315169367 20.39764359351988
684 42 22.29337602561537 28.036822093255953 27.71662997798679 21.953171903141886 135 74-75 18.372606774668633 28.019145802650957 34.5360824742268 19.072164948453608
685 43 21.244248849769953 28.425685137027408 27.825565113022606 22.504500900180034 136 76-77 16.678939617083945 31.70103092783505 31.73784977908689 19.88217967599411
686 44 21.404280856171233 28.985797159431886 27.545509101820365 22.064412882576516 137 78-79 18.77761413843888 28.387334315169365 32.62150220913107 20.213549337260677
687 45 21.86 29.28 26.740000000000002 22.12 138 80-81 16.384388807069218 30.927835051546392 32.9160530191458 19.771723122238587
688 46 21.26 27.54 27.74 23.46 139 82-83 17.083946980854197 30.927835051546392 32.54786450662739 19.44035346097202
689 47 21.628651460584233 28.271308523409367 28.051220488195277 22.048819527811126 140 84-85 16.936671575846834 31.590574374079527 31.443298969072163 20.029455081001473
690 48 21.279999999999998 28.439999999999998 26.400000000000002 23.880000000000003 141 86-87 17.488954344624446 27.908689248895435 34.20471281296024 20.39764359351988
691 49 21.044208841768352 29.045809161832363 27.185437087417487 22.724544908981798 142 88-89 16.789396170839467 32.36377025036819 31.075110456553755 19.771723122238587
692 50 21.82873149259704 28.15126050420168 26.810724289715886 23.209283713485394 143 90-91 19.587628865979383 29.749631811487482 33.5419734904271 17.120765832106038
693 51 21.028411364545818 27.73109243697479 28.011204481792717 23.229291716686674 144 92-93 17.746686303387335 30.04418262150221 31.33284241531664 20.876288659793815
694 52 21.824364872974595 30.146029205841167 25.94518903780756 22.084416883376676 145 94-95 18.924889543446245 28.460972017673047 33.39469808541973 19.219440353460975
695 53 21.164232846569313 28.325665133026607 28.045609121824366 22.464492898579717 146 96-97 16.42120765832106 29.418262150220915 32.47422680412371 21.686303387334316
696 54 21.53907976692787 27.024311834438418 26.90375728350412 24.5328511151296 147 98-99 18.158090976882924 29.34377330350485 33.1469052945563 19.351230425055927
148 100-101 17.77358490566038 29.849056603773583 33.28301886792453 19.09433962264151
149 102-103 18.339622641509436 29.509433962264154 30.90566037735849 21.245283018867926
150 104-105 18.037735849056606 28.67924528301887 31.77358490566038 21.50943396226415
151 106-107 17.2782874617737 29.357798165137616 33.52446483180428 19.839449541284402
152 108 20.053475935828878 26.38146167557932 31.72905525846702 21.836007130124777
697 >>END_MODULE 153 >>END_MODULE
698 >>Per sequence GC content warn 154 >>Per sequence GC content warn
699 #GC Content Count 155 #GC Content Count
700 0 3.0 156 0 15.0
701 1 5.0 157 1 15.5
702 2 7.0 158 2 16.5
703 3 7.0 159 3 17.0
704 4 7.0 160 4 18.0
705 5 5.0 161 5 21.5
706 6 2.0 162 6 26.5
707 7 1.0 163 7 30.0
708 8 1.5 164 8 33.5
709 9 2.0 165 9 36.0
710 10 1.5 166 10 41.0
711 11 1.0 167 11 47.0
712 12 2.5 168 12 47.5
713 13 4.0 169 13 56.0
714 14 4.0 170 14 65.5
715 15 4.0 171 15 69.0
716 16 5.0 172 16 72.5
717 17 6.0 173 17 78.0
718 18 11.0 174 18 86.5
719 19 19.5 175 19 94.5
720 20 23.0 176 20 105.5
721 21 37.5 177 21 113.0
722 22 52.0 178 22 123.0
723 23 61.0 179 23 132.5
724 24 70.0 180 24 149.5
725 25 79.0 181 25 172.5
726 26 88.0 182 26 200.0
727 27 117.5 183 27 222.0
728 28 147.0 184 28 247.0
729 29 159.5 185 29 282.0
730 30 172.0 186 30 310.5
731 31 203.0 187 31 337.0
732 32 243.0 188 32 370.0
733 33 252.0 189 33 403.5
734 34 290.0 190 34 438.0
735 35 328.0 191 35 465.0
736 36 330.0 192 36 481.5
737 37 332.0 193 37 511.0
738 38 332.5 194 38 523.5
739 39 333.0 195 39 506.0
740 40 342.0 196 40 491.0
741 41 351.0 197 41 491.5
742 42 343.5 198 42 482.0
743 43 336.0 199 43 483.0
744 44 318.5 200 44 487.5
745 45 279.5 201 45 471.5
746 46 258.0 202 46 471.0
747 47 254.5 203 47 468.5
748 48 251.0 204 48 473.5
749 49 234.5 205 49 472.0
750 50 218.0 206 50 437.5
751 51 209.5 207 51 415.5
752 52 201.0 208 52 407.5
753 53 198.5 209 53 398.0
754 54 196.0 210 54 381.0
755 55 177.0 211 55 364.0
756 56 162.5 212 56 349.0
757 57 167.0 213 57 341.0
758 58 147.0 214 58 330.5
759 59 127.0 215 59 320.0
760 60 106.5 216 60 320.5
761 61 86.0 217 61 301.0
762 62 85.0 218 62 275.0
763 63 84.0 219 63 245.0
764 64 61.5 220 64 207.0
765 65 39.0 221 65 190.5
766 66 42.5 222 66 182.0
767 67 46.0 223 67 173.0
768 68 34.0 224 68 167.0
769 69 17.0 225 69 151.5
770 70 12.0 226 70 131.5
771 71 14.0 227 71 121.0
772 72 16.0 228 72 117.5
773 73 12.5 229 73 110.5
774 74 9.0 230 74 104.0
775 75 6.5 231 75 90.5
776 76 4.0 232 76 75.0
777 77 4.5 233 77 67.5
778 78 5.0 234 78 62.5
779 79 5.0 235 79 61.5
780 80 5.0 236 80 59.5
781 81 3.0 237 81 57.0
782 82 1.0 238 82 54.5
783 83 1.0 239 83 47.0
784 84 1.0 240 84 39.5
785 85 1.0 241 85 38.0
786 86 1.0 242 86 36.5
787 87 1.0 243 87 35.5
788 88 1.0 244 88 28.0
789 89 1.0 245 89 21.0
790 90 2.0 246 90 19.5
791 91 3.0 247 91 17.0
792 92 2.5 248 92 15.5
793 93 2.0 249 93 14.5
794 94 2.0 250 94 13.5
795 95 3.0 251 95 13.5
796 96 4.0 252 96 15.0
797 97 5.0 253 97 15.5
798 98 6.0 254 98 17.0
799 99 3.5 255 99 16.0
800 100 1.0 256 100 13.5
801 >>END_MODULE 257 >>END_MODULE
802 >>Per base N content pass 258 >>Per base N content pass
803 #Base N-Count 259 #Base N-Count
804 1 0.0 260 1 0.0
805 2 0.0 261 2 0.0
808 5 0.0 264 5 0.0
809 6 0.0 265 6 0.0
810 7 0.0 266 7 0.0
811 8 0.0 267 8 0.0
812 9 0.0 268 9 0.0
813 10 0.0 269 10-11 0.0
814 11 0.0 270 12-13 0.0
815 12 0.0 271 14-15 0.0
816 13 0.0 272 16-17 0.0
817 14 0.0 273 18-19 0.0
818 15 0.0 274 20-21 0.0
819 16 0.0 275 22-23 0.0
820 17 0.0 276 24-25 0.0
821 18 0.0 277 26-27 0.0
822 19 0.0 278 28-29 0.0
823 20 0.0 279 30-31 0.0
824 21 0.0 280 32-33 0.0
825 22 0.0 281 34-35 0.0
826 23 0.0 282 36-37 0.0
827 24 0.0 283 38-39 0.0
828 25 0.0 284 40-41 0.0
829 26 0.04 285 42-43 0.0
830 27 0.02 286 44-45 0.0
831 28 0.0 287 46-47 0.0
832 29 0.02 288 48-49 0.0
833 30 0.0 289 50-51 0.0
834 31 0.0 290 52-53 0.0
835 32 0.04 291 54-55 0.0
836 33 0.02 292 56-57 0.0
837 34 0.02 293 58-59 0.0
838 35 0.02 294 60-61 0.0
839 36 0.02 295 62-63 0.0
840 37 0.04 296 64-65 0.0
841 38 0.02 297 66-67 0.0
842 39 0.04 298 68-69 0.0
843 40 0.0 299 70-71 0.0
844 41 0.06 300 72-73 0.0
845 42 0.06 301 74-75 0.0
846 43 0.02 302 76-77 0.0
847 44 0.02 303 78-79 0.0
848 45 0.0 304 80-81 0.0
849 46 0.0 305 82-83 0.0
850 47 0.04 306 84-85 0.0
851 48 0.0 307 86-87 0.0
852 49 0.02 308 88-89 0.0
853 50 0.04 309 90-91 0.0
854 51 0.04 310 92-93 0.0
855 52 0.02 311 94-95 0.0
856 53 0.02 312 96-97 0.0
857 54 0.45999999999999996 313 98-99 0.0
858 >>END_MODULE 314 100-101 0.0
859 >>Sequence Length Distribution pass 315 102-103 0.0
316 104-105 0.0
317 106-107 0.0
318 108 0.0
319 >>END_MODULE
320 >>Sequence Length Distribution warn
860 #Length Count 321 #Length Count
861 54 5000.0 322 0-4 98.0
323 5-9 250.0
324 10-14 257.0
325 15-19 279.0
326 20-24 321.0
327 25-29 396.0
328 30-34 401.0
329 35-39 433.0
330 40-44 401.0
331 45-49 385.0
332 50-54 320.0
333 55-59 5.0
334 60-64 1.0
335 65-69 0.0
336 70-74 0.0
337 75-79 0.0
338 80-84 0.0
339 85-89 0.0
340 90-94 0.0
341 95-99 33.0
342 100-104 0.0
343 105-109 1325.0
862 >>END_MODULE 344 >>END_MODULE
863 >>Sequence Duplication Levels pass 345 >>Sequence Duplication Levels pass
864 #Total Deduplicated Percentage 99.52 346 #Total Deduplicated Percentage 98.73598369011212
865 #Duplication Level Percentage of deduplicated Percentage of total 347 #Duplication Level Percentage of deduplicated Percentage of total
866 1 99.81913183279742 99.33999999999999 348 1 99.44249432170142 98.1855249745158
867 2 0.08038585209003216 0.16 349 2 0.47491224447656405 0.9378185524974516
868 3 0.04019292604501608 0.12 350 3 0.041296716911005574 0.12232415902140673
869 4 0.0 0.0 351 4 0.020648358455502787 0.08154943934760449
870 5 0.02009646302250804 0.1 352 5 0.0 0.0
871 6 0.0 0.0 353 6 0.0 0.0
872 7 0.04019292604501608 0.27999999999999997 354 7 0.0 0.0
873 8 0.0 0.0 355 8 0.0 0.0
874 9 0.0 0.0 356 9 0.0 0.0
875 >10 0.0 0.0 357 >10 0.020648358455502787 0.672782874617737
876 >50 0.0 0.0 358 >50 0.0 0.0
877 >100 0.0 0.0 359 >100 0.0 0.0
878 >500 0.0 0.0 360 >500 0.0 0.0
879 >1k 0.0 0.0 361 >1k 0.0 0.0
880 >5k 0.0 0.0 362 >5k 0.0 0.0
881 >10k+ 0.0 0.0 363 >10k+ 0.0 0.0
882 >>END_MODULE 364 >>END_MODULE
883 >>Overrepresented sequences warn 365 >>Overrepresented sequences warn
884 #Sequence Count Percentage Possible Source 366 #Sequence Count Percentage Possible Source
885 CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT 7 0.13999999999999999 Illumina Paired End PCR Primer 2 (96% over 33bp) 367 ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672782874617737 No Hit
886 CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC 7 0.13999999999999999 Illumina Paired End PCR Primer 2 (100% over 35bp)
887 >>END_MODULE 368 >>END_MODULE
888 >>Adapter Content pass 369 >>Adapter Content pass
889 #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 370 #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter
890 1 0.0 0.0 0.0 0.0 0.0 371 1 0.0 0.0 0.0 0.0 0.0
891 2 0.02 0.0 0.0 0.0 0.0 372 2 0.0 0.0 0.0 0.0 0.0
892 3 0.02 0.0 0.0 0.0 0.0 373 3 0.0 0.0 0.0 0.0 0.0
893 4 0.02 0.0 0.0 0.0 0.0 374 4 0.0 0.0 0.0 0.0 0.0
894 5 0.02 0.0 0.0 0.0 0.0 375 5 0.0 0.0 0.0 0.0 0.0
895 6 0.02 0.0 0.0 0.0 0.0 376 6 0.0 0.0 0.0 0.0 0.0
896 7 0.02 0.0 0.0 0.0 0.0 377 7 0.0 0.0 0.0 0.0 0.0
897 8 0.02 0.0 0.0 0.0 0.0 378 8 0.0 0.0 0.0 0.0 0.0
898 9 0.02 0.0 0.0 0.0 0.0 379 9 0.0 0.0 0.0 0.0 0.0
899 10 0.02 0.0 0.0 0.0 0.0 380 10-11 0.0 0.0 0.0 0.0 0.0
900 11 0.02 0.0 0.0 0.0 0.0 381 12-13 0.0 0.0 0.0 0.0 0.0
901 12 0.02 0.0 0.0 0.0 0.0 382 14-15 0.0 0.0 0.0 0.0 0.0
902 13 0.02 0.0 0.0 0.0 0.0 383 16-17 0.0 0.0 0.0 0.0 0.0
903 14 0.02 0.0 0.0 0.0 0.0 384 18-19 0.0 0.0 0.0 0.0 0.0
904 15 0.02 0.0 0.0 0.0 0.0 385 20-21 0.12232415902140673 0.0 0.0 0.0 0.0
905 16 0.02 0.0 0.0 0.0 0.0 386 22-23 0.12232415902140673 0.0 0.0 0.0 0.0
906 17 0.02 0.0 0.0 0.0 0.0 387 24-25 0.12232415902140673 0.0 0.0 0.0 0.0
907 18 0.02 0.0 0.0 0.0 0.0 388 26-27 0.1325178389398573 0.0 0.0 0.0 0.0
908 19 0.06 0.0 0.0 0.0 0.0 389 28-29 0.2038735983690112 0.0 0.0 0.0 0.0
909 20 0.36 0.0 0.0 0.0 0.0 390 30-31 0.22426095820591233 0.0 0.0 0.0 0.0
910 21 0.36 0.0 0.0 0.0 0.0 391 32-33 0.22426095820591233 0.0 0.0 0.0 0.0
911 22 0.36 0.0 0.0 0.0 0.0 392 34-35 0.24464831804281345 0.0 0.0 0.0 0.0
912 23 0.36 0.0 0.0 0.0 0.0 393 36-37 0.3058103975535168 0.0 0.0 0.0 0.0
913 24 0.36 0.0 0.0 0.0 0.0 394 38-39 0.4383282364933741 0.0 0.0 0.0 0.0
914 25 0.4 0.0 0.0 0.0 0.0 395 40-41 0.4689092762487258 0.0 0.0 0.0 0.0
915 26 0.4 0.0 0.0 0.0 0.0 396 42-43 0.4689092762487258 0.0 0.0 0.0 0.0
916 27 0.42 0.0 0.0 0.0 0.0 397 44-45 0.4689092762487258 0.0 0.0 0.0 0.0
917 28 0.62 0.0 0.0 0.0 0.0 398 46-47 0.4689092762487258 0.0 0.0 0.0 0.0
918 29 0.66 0.0 0.0 0.0 0.0 399 48-49 0.4689092762487258 0.0 0.0 0.0 0.0
919 30 0.68 0.0 0.0 0.0 0.0 400 50-51 0.4689092762487258 0.0 0.0 0.0 0.0
920 31 0.68 0.0 0.0 0.0 0.0 401 52-53 0.4689092762487258 0.0 0.0 0.0 0.0
921 32 0.68 0.0 0.0 0.0 0.0 402 54-55 0.4689092762487258 0.0 0.0 0.0 0.0
922 33 0.68 0.0 0.0 0.0 0.0 403 56-57 0.4689092762487258 0.0 0.0 0.0 0.0
923 34 0.7 0.0 0.0 0.0 0.0 404 58-59 0.4689092762487258 0.0 0.0 0.0 0.0
924 35 0.76 0.0 0.0 0.0 0.0 405 60-61 0.4689092762487258 0.0 0.0 0.0 0.0
925 36 0.8 0.0 0.0 0.0 0.0 406 62-63 0.4689092762487258 0.0 0.0 0.0 0.0
926 37 0.88 0.0 0.0 0.0 0.0 407 64-65 0.4689092762487258 0.0 0.0 0.0 0.0
927 38 1.0 0.0 0.0 0.0 0.0 408 66-67 0.4689092762487258 0.0 0.0 0.0 0.0
928 39 1.12 0.0 0.0 0.0 0.0 409 68-69 0.4689092762487258 0.0 0.0 0.0 0.0
929 40 1.22 0.0 0.0 0.0 0.0 410 70-71 0.4689092762487258 0.0 0.0 0.0 0.0
930 41 1.38 0.0 0.0 0.0 0.0 411 72-73 0.4689092762487258 0.0 0.0 0.0 0.0
931 42 1.48 0.0 0.0 0.0 0.0 412 74-75 0.4689092762487258 0.0 0.0 0.0 0.0
932 43 1.68 0.0 0.0 0.0 0.0 413 76-77 0.4689092762487258 0.0 0.0 0.0 0.0
933 >>END_MODULE 414 78-79 0.4689092762487258 0.0 0.0 0.0 0.0
415 80-81 0.4689092762487258 0.0 0.0 0.0 0.0
416 82-83 0.4689092762487258 0.0 0.0 0.0 0.0
417 84-85 0.4689092762487258 0.0 0.0 0.0 0.0
418 86-87 0.4689092762487258 0.0 0.0 0.0 0.0
419 88-89 0.4689092762487258 0.0 0.0 0.0 0.0
420 90-91 0.4689092762487258 0.0 0.0 0.0 0.0
421 92-93 0.4689092762487258 0.0 0.0 0.0 0.0
422 94-95 0.4689092762487258 0.0 0.0 0.0 0.0
423 96-97 0.47910295616717635 0.0 0.0 0.0 0.0
424 >>END_MODULE