Mercurial > repos > devteam > fastqc
diff test-data/fastqc_customlimits.txt @ 1:8fae48caaf06 draft
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author | devteam |
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date | Tue, 11 Nov 2014 12:46:27 -0500 |
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children | a00a6402d09a |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastqc_customlimits.txt Tue Nov 11 12:46:27 2014 -0500 @@ -0,0 +1,84 @@ +# For each of the modules you can choose to not run that +# module at all by setting the value below to 1 for the +# modules you want to remove. +duplication ignore 1 +kmer ignore 0 +n_content ignore 0 +overrepresented ignore 0 +quality_base ignore 0 +sequence ignore 0 +gc_sequence ignore 0 +quality_sequence ignore 1 +tile ignore 1 +sequence_length ignore 0 +adapter ignore 0 + +# For the duplication module the value is the percentage +# remaining after deduplication. Measured levels below +# these limits trigger the warning / error. +duplication warn 70 +duplication error 50 + +# For the kmer module the filter is on the -log10 binomial +# pvalue for the most significant Kmer, so 5 would be +# 10^-5 = p<0.00001 +kmer warn 2 +kmer error 5 + +# For the N module the filter is on the percentage of Ns +# at any position in the library +n_content warn 5 +n_content error 20 + +# For the overrepresented seqs the warn value sets the +# threshold for the overrepresented sequences to be reported +# at all as the proportion of the library which must be seen +# as a single sequence +overrepresented warn 0.001 +overrepresented error 1 + +# The per base quality filter uses two values, one for the value +# of the lower quartile, and the other for the value of the +# median quality. Failing either of these will trigger the alert +quality_base_lower warn 10 +quality_base_lower error 5 +quality_base_median warn 50 +quality_base_median error 20 + +# The per base sequence content module tests the maximum deviation +# between A and T or C and G +sequence warn 2 +sequence error 5 + +# The per sequence GC content tests the maximum deviation between +# the theoretical distribution and the real distribution +gc_sequence warn 15 +gc_sequence error 30 + +# The per sequence quality module tests the phred score which is +# most frequently observed +quality_sequence warn 27 +quality_sequence error 20 + +# The per tile module tests the maximum phred score loss between +# and individual tile and the average for that base across all tiles +tile warn 5 +tile error 10 + +# The sequence length module tests are binary, so the values here +# simply turn them on or off. The actual tests warn if you have +# sequences of different length, and error if you have sequences +# of zero length. + +sequence_length warn 1 +sequence_length error 1 + +# The adapter module's warnings and errors are based on the +# percentage of reads in the library which have been observed +# to contain an adapter associated Kmer at any point + +adapter warn 5 +adapter error 10 + + + \ No newline at end of file