Mercurial > repos > devteam > fastqc
diff test-data/fastqc_data.txt @ 19:9da02be9c6cc draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastqc commit 2f544e337886709995a93d53f394919ce8e4673a
| author | iuc |
|---|---|
| date | Fri, 10 May 2019 14:23:53 -0400 |
| parents | 3e1cdf5406db |
| children | ddf5c37952ac |
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--- a/test-data/fastqc_data.txt Mon Nov 05 12:08:29 2018 -0500 +++ b/test-data/fastqc_data.txt Fri May 10 14:23:53 2019 -0400 @@ -1,259 +1,803 @@ -##FastQC 0.11.8 +##FastQC 0.11.6 >>Basic Statistics pass #Measure Value -Filename 1000trimmed_fastq +Filename 1000gsample_fastq_bz2.bz2 File type Conventional base calls Encoding Sanger / Illumina 1.9 -Total Sequences 4905 +Total Sequences 5000 Sequences flagged as poor quality 0 -Sequence length 1-108 -%GC 40 +Sequence length 54 +%GC 43 >>END_MODULE ->>Per base sequence quality pass +>>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile -1 29.282772680937818 31.0 27.0 33.0 23.0 34.0 -2 28.832925336597306 30.0 27.0 32.0 22.0 33.0 -3 28.896953588223266 30.0 27.0 32.0 23.0 34.0 -4 29.006785934608267 30.0 27.0 32.0 23.0 34.0 -5 28.9377990430622 30.0 27.0 32.0 22.0 34.0 -6 29.002938706968934 30.0 27.0 32.0 23.0 34.0 -7 28.899830220713074 30.0 27.0 32.0 22.0 34.0 -8 28.900706184463942 30.0 27.0 32.0 22.0 33.0 -9 28.754602555772145 30.0 27.0 32.0 22.0 34.0 -10-11 28.822083022456294 30.0 27.0 32.0 22.5 33.0 -12-13 28.74898578710789 30.0 26.5 32.0 22.0 33.0 -14-15 28.53370750727077 30.0 26.0 32.0 22.0 33.5 -16-17 28.52661127583153 30.0 26.0 32.0 22.0 33.0 -18-19 28.68343192244741 30.0 26.0 32.0 22.5 33.0 -20-21 28.370130166336985 29.5 26.0 32.0 22.0 33.0 -22-23 28.185946266098476 29.0 26.0 32.0 22.0 33.0 -24-25 28.13009469533431 29.0 26.0 32.0 21.5 33.0 -26-27 28.075768267577 29.0 26.0 32.0 21.5 33.0 -28-29 27.828024188665616 29.0 25.0 32.0 21.0 33.0 -30-31 27.792826876513317 29.0 25.0 31.5 21.0 33.0 -32-33 27.490989073616355 28.0 25.0 31.0 21.0 33.0 -34-35 27.357199679261562 28.0 24.5 31.0 21.0 33.0 -36-37 27.062158129410527 28.0 24.0 31.0 20.5 33.0 -38-39 27.238022216783758 28.0 24.0 31.0 21.0 33.0 -40-41 27.028963434226593 28.0 24.0 31.0 20.5 33.0 -42-43 26.90862078496675 27.0 24.0 31.0 20.5 33.0 -44-45 26.632003369751054 27.0 24.0 30.0 20.0 32.0 -46-47 26.83042786373109 27.5 24.0 31.0 20.5 32.0 -48-49 26.431681832255798 27.0 23.5 30.0 20.0 32.0 -50-51 26.219000735104444 27.0 23.0 30.0 20.0 32.0 -52-53 25.925690423864786 26.5 22.5 29.5 19.5 31.5 -54-55 27.995241016389798 29.5 25.5 31.5 20.5 33.0 -56-57 30.410857897242785 31.5 28.5 33.0 25.5 34.0 -58-59 30.4083885209713 31.5 28.5 33.0 25.5 34.0 -60-61 30.444812362030905 31.5 29.0 33.0 26.0 34.0 -62-63 30.44039735099338 31.5 29.0 33.0 25.5 34.0 -64-65 30.441712697003883 31.5 29.0 33.0 25.5 34.0 -66-67 30.51141384388807 32.0 29.0 33.0 25.5 34.0 -68-69 30.05117820324006 31.0 28.0 33.0 24.5 34.0 -70-71 30.276141384388808 31.0 29.0 33.0 25.0 34.0 -72-73 30.385493372606774 31.5 29.0 33.0 25.5 34.0 -74-75 30.213549337260677 31.0 28.5 33.0 25.5 34.0 -76-77 29.678939617083948 31.0 28.0 33.0 24.5 34.0 -78-79 30.019145802650957 31.0 28.0 33.0 24.5 34.0 -80-81 29.664212076583212 31.0 27.5 33.0 24.0 34.0 -82-83 29.653534609720175 31.0 28.0 32.5 24.5 34.0 -84-85 29.425625920471283 30.5 27.0 32.0 24.0 34.0 -86-87 29.34057437407953 30.0 27.0 32.5 24.0 34.0 -88-89 28.785714285714285 30.0 27.0 32.0 22.5 33.5 -90-91 28.667525773195877 29.5 26.0 32.0 23.0 33.0 -92-93 28.565905743740796 29.0 26.0 32.0 23.0 33.0 -94-95 28.271723122238583 29.0 26.0 32.0 22.5 33.0 -96-97 27.94955817378498 29.0 25.5 31.0 22.0 33.0 -98-99 27.641132075471695 28.0 25.0 31.0 21.5 33.0 -100-101 27.614716981132077 28.0 25.0 31.0 21.5 33.0 -102-103 27.196226415094337 28.0 24.5 31.0 21.0 32.5 -104-105 26.870943396226416 27.5 24.0 31.0 20.0 32.5 -106-107 26.367593937711003 27.0 23.5 30.0 20.5 32.0 -108 27.5650623885918 28.0 24.0 31.0 22.0 33.0 +1 28.9946 31.0 27.0 33.0 22.0 34.0 +2 28.486 30.0 26.0 32.0 21.0 33.0 +3 28.5272 30.0 26.0 32.0 21.0 34.0 +4 28.6252 30.0 26.0 32.0 22.0 33.0 +5 28.4804 30.0 26.0 32.0 21.0 33.0 +6 28.5128 30.0 26.0 32.0 22.0 33.0 +7 28.4102 30.0 26.0 32.0 21.0 33.0 +8 28.4674 30.0 26.0 32.0 22.0 33.0 +9 28.182 30.0 26.0 32.0 21.0 33.0 +10 28.0908 30.0 26.0 32.0 21.0 33.0 +11 28.1876 30.0 26.0 32.0 21.0 33.0 +12 28.1742 30.0 26.0 32.0 21.0 33.0 +13 27.923 30.0 25.0 32.0 20.0 33.0 +14 27.8984 29.0 25.0 32.0 21.0 33.0 +15 27.6112 29.0 25.0 32.0 20.0 33.0 +16 27.6638 29.0 25.0 32.0 20.0 33.0 +17 27.5734 29.0 25.0 32.0 20.0 33.0 +18 27.6348 29.0 25.0 32.0 20.0 33.0 +19 27.6526 29.0 25.0 32.0 20.0 33.0 +20 27.2716 29.0 24.0 32.0 19.0 33.0 +21 27.2642 29.0 25.0 31.0 19.0 33.0 +22 26.881 28.0 24.0 31.0 19.0 33.0 +23 26.8124 28.0 24.0 31.0 19.0 33.0 +24 26.8204 28.0 24.0 31.0 19.0 33.0 +25 26.6606 28.0 24.0 31.0 18.0 33.0 +26 26.3806 28.0 23.0 31.0 18.0 33.0 +27 25.947 28.0 23.0 31.0 17.0 33.0 +28 25.7446 27.0 22.0 31.0 16.0 33.0 +29 25.6856 27.0 22.0 31.0 16.0 33.0 +30 25.5228 27.0 22.0 31.0 16.0 33.0 +31 25.354 27.0 22.0 30.0 16.0 32.0 +32 25.1656 27.0 22.0 30.0 16.0 32.0 +33 25.0164 26.0 21.0 30.0 15.0 32.0 +34 24.75 26.0 21.0 30.0 15.0 32.0 +35 24.3926 26.0 21.0 30.0 15.0 32.0 +36 24.1122 25.0 20.0 30.0 14.0 32.0 +37 23.8612 25.0 20.0 29.0 13.0 32.0 +38 23.7544 25.0 20.0 29.0 13.0 32.0 +39 23.485 25.0 19.0 29.0 13.0 32.0 +40 23.1984 24.0 19.0 29.0 13.0 32.0 +41 23.0976 24.0 19.0 29.0 13.0 32.0 +42 22.9212 24.0 19.0 28.0 13.0 31.0 +43 22.5172 24.0 18.0 28.0 12.0 31.0 +44 22.3234 23.0 18.0 28.0 12.0 31.0 +45 22.1372 23.0 18.0 28.0 11.0 31.0 +46 22.2166 23.0 18.0 28.0 11.0 31.0 +47 22.075 23.0 18.0 28.0 11.0 31.0 +48 21.4592 22.0 17.0 27.0 11.0 31.0 +49 21.0954 22.0 16.0 27.0 10.0 30.0 +50 20.9356 22.0 16.0 27.0 10.0 30.0 +51 20.6152 21.0 15.0 27.0 9.0 30.0 +52 20.6588 21.0 16.0 27.0 9.0 30.0 +53 19.5852 20.0 14.0 25.0 9.0 29.0 +54 19.9424 21.0 14.0 26.0 7.0 30.0 +>>END_MODULE +>>Per tile sequence quality pass +#Tile Base Mean +1 1 0.4542485249902164 +1 2 0.02493171505959424 +1 3 0.004493134032774293 +1 4 0.6119404948560927 +1 5 0.5283275008594686 +1 6 0.3741933003058371 +1 7 -0.2985554485298749 +1 8 0.6040181642739206 +1 9 0.43795745033596845 +1 10 0.05327706110314523 +1 11 0.8097461135057031 +1 12 0.9171964750225641 +1 13 1.0716973449965757 +1 14 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2.981199715317363 +10 36 0.8110078355858406 +10 37 1.8617452936378776 +10 38 1.7742605987363014 +10 39 3.2937897396720928 +10 40 3.2918952034041524 +10 41 1.3115749098869323 +10 42 2.911702897841007 +10 43 2.9292302270051636 +10 44 1.964400342482186 +10 45 0.5477757053971892 +10 46 1.9135298205630704 +10 47 1.2741568491184836 +10 48 2.799334226444202 +10 49 3.441999390209105 +10 50 4.391043406721156 +10 51 3.7815999232367545 +10 52 1.3217607371315836 +10 53 3.0125314696670173 +10 54 2.2316249763308633 >>END_MODULE >>Per sequence quality scores warn #Quality Count -20 7.0 -21 24.0 -22 47.0 -23 78.0 -24 226.0 -25 513.0 -26 830.0 -27 1017.0 -28 947.0 -29 645.0 -30 352.0 -31 157.0 -32 55.0 -33 6.0 -34 1.0 +8 4.0 +9 4.0 +10 2.0 +11 2.0 +12 4.0 +13 6.0 +14 4.0 +15 11.0 +16 20.0 +17 50.0 +18 98.0 +19 149.0 +20 210.0 +21 275.0 +22 367.0 +23 425.0 +24 518.0 +25 540.0 +26 539.0 +27 532.0 +28 468.0 +29 360.0 +30 234.0 +31 122.0 +32 51.0 +33 5.0 >>END_MODULE >>Per base sequence content pass #Base G A T C -1 21.223241590214066 33.00713557594291 25.97349643221203 19.79612640163099 -2 23.76580987352101 31.415748674010608 27.315381476948186 17.503059975520195 -3 21.876916785933346 28.70578613780413 29.46227765283173 19.95501942343079 -4 21.55048324079786 28.295290972650626 28.850503804236066 21.303721982315444 -5 21.219055543998337 30.205949656750576 28.271271063033076 20.303723736218014 -6 22.124265323257767 28.946263643996637 26.364399664147776 22.565071368597817 -7 21.943972835314092 29.9660441426146 28.395585738539896 19.69439728353141 -8 21.378129681147016 28.825165846351382 27.8621870318853 21.934517440616304 -9 20.077972709551656 29.088152479965345 28.654970760233915 22.17890405024908 -10-11 21.243237275035884 29.468919068124105 27.680247322512976 21.607596334327038 -12-13 20.8276950834461 29.03698691926026 29.18358141632837 20.951736580965267 -14-15 20.24001846295869 28.975305792753286 29.471497807523654 21.313177936764365 -16-17 19.83872880351002 29.396418830783826 29.621724178821296 21.14312818688486 -18-19 20.845552297165202 27.871456500488755 29.973118279569892 21.309872922776147 -20-21 20.538172715894866 28.973717146433042 29.111389236545683 21.376720901126408 -22-23 20.88082901554404 30.129533678756477 29.300518134715027 19.689119170984455 -24-25 20.506974248927037 29.07725321888412 29.05042918454936 21.365343347639485 -26-27 20.86603139013453 28.054932735426007 30.339125560538115 20.739910313901344 -28-29 19.32600732600733 30.842490842490843 28.776556776556777 21.054945054945055 -30-31 20.638820638820636 29.19226044226044 29.49938574938575 20.669533169533167 -32-33 19.47989016314004 29.009853012437407 29.38136003876595 22.1288967856566 -34-35 19.6674020023757 29.713219073477003 30.205328355676226 20.41405056847107 -36-37 20.443482963764197 29.853975121687398 30.61114115738237 19.091400757166035 -38-39 20.992661259173424 28.041714947856317 30.088837388953266 20.876786404016993 -40-41 19.860197368421055 28.680098684210524 30.32483552631579 21.134868421052634 -42-43 20.01321003963012 27.873183619550858 30.823425803610743 21.29018053720828 -44-45 18.941371943982908 30.192262046047947 29.954901495371466 20.911464514597675 -46-47 20.1888718734048 28.126595201633485 31.470137825421133 20.21439509954058 -48-49 19.56159822419534 29.689234184239734 28.77358490566038 21.975582685904552 -50-51 20.50656087885261 28.2575526396094 30.759841318278912 20.47604516325908 -52-53 17.691256830601095 29.918032786885245 31.864754098360653 20.525956284153004 -54-55 17.214912280701753 32.30994152046784 29.53216374269006 20.942982456140353 -56-57 19.30856932695844 30.893710923133504 30.893710923133504 18.90400882677455 -58-59 20.014716703458426 29.72774098601913 31.42016188373804 18.8373804267844 -60-61 17.402501839587934 33.18616629874908 29.506990434142754 19.904341427520237 -62-63 18.543046357615893 29.72774098601913 32.2663723325975 19.462840323767477 -64-65 16.893632683106365 32.94074346705926 30.180345969819655 19.985277880014724 -66-67 18.519882179675996 28.902798232695137 32.80559646539028 19.771723122238587 -68-69 17.2680412371134 30.375552282768776 31.66421207658321 20.692194403534607 -70-71 17.673048600883654 27.798232695139912 35.34609720176731 19.182621502209134 -72-73 16.200294550810014 29.528718703976438 33.87334315169367 20.39764359351988 -74-75 18.372606774668633 28.019145802650957 34.5360824742268 19.072164948453608 -76-77 16.678939617083945 31.70103092783505 31.73784977908689 19.88217967599411 -78-79 18.77761413843888 28.387334315169365 32.62150220913107 20.213549337260677 -80-81 16.384388807069218 30.927835051546392 32.9160530191458 19.771723122238587 -82-83 17.083946980854197 30.927835051546392 32.54786450662739 19.44035346097202 -84-85 16.936671575846834 31.590574374079527 31.443298969072163 20.029455081001473 -86-87 17.488954344624446 27.908689248895435 34.20471281296024 20.39764359351988 -88-89 16.789396170839467 32.36377025036819 31.075110456553755 19.771723122238587 -90-91 19.587628865979383 29.749631811487482 33.5419734904271 17.120765832106038 -92-93 17.746686303387335 30.04418262150221 31.33284241531664 20.876288659793815 -94-95 18.924889543446245 28.460972017673047 33.39469808541973 19.219440353460975 -96-97 16.42120765832106 29.418262150220915 32.47422680412371 21.686303387334316 -98-99 18.158090976882924 29.34377330350485 33.1469052945563 19.351230425055927 -100-101 17.77358490566038 29.849056603773583 33.28301886792453 19.09433962264151 -102-103 18.339622641509436 29.509433962264154 30.90566037735849 21.245283018867926 -104-105 18.037735849056606 28.67924528301887 31.77358490566038 21.50943396226415 -106-107 17.2782874617737 29.357798165137616 33.52446483180428 19.839449541284402 -108 20.053475935828878 26.38146167557932 31.72905525846702 21.836007130124777 +1 21.5 32.64 25.919999999999998 19.939999999999998 +2 24.04 31.72 26.540000000000003 17.7 +3 21.58 28.860000000000003 29.42 20.14 +4 21.58 28.28 28.9 21.240000000000002 +5 21.16 29.720000000000002 28.32 20.8 +6 22.38 29.24 25.72 22.66 +7 21.34 30.320000000000004 28.24 20.1 +8 21.58 29.060000000000002 27.939999999999998 21.42 +9 20.3 28.48 28.58 22.64 +10 21.2 30.84 26.56 21.4 +11 21.14 28.64 27.279999999999998 22.939999999999998 +12 21.32 28.88 28.999999999999996 20.8 +13 20.36 29.220000000000002 28.939999999999998 21.48 +14 20.02 29.2 29.220000000000002 21.560000000000002 +15 20.200000000000003 29.220000000000002 28.38 22.2 +16 20.34 29.38 29.299999999999997 20.979999999999997 +17 20.599999999999998 29.14 28.499999999999996 21.759999999999998 +18 20.599999999999998 27.92 29.4 22.08 +19 21.66 28.46 28.58 21.3 +20 20.0 29.24 28.96 21.8 +21 21.72 28.499999999999996 28.18 21.6 +22 20.52 30.880000000000003 27.3 21.3 +23 20.72 28.9 29.720000000000002 20.66 +24 21.12 28.62 27.58 22.68 +25 21.099999999999998 29.060000000000002 29.160000000000004 20.68 +26 22.18887555022009 28.391356542617046 28.131252501000397 21.288515406162464 +27 21.344268853770753 28.525705141028208 28.765753150630125 21.364272854570913 +28 19.34 30.54 27.46 22.66 +29 20.90418083616723 29.12582516503301 29.045809161832363 20.924184836967395 +30 21.099999999999998 29.4 27.439999999999998 22.06 +31 20.84 28.04 28.660000000000004 22.46 +32 19.847939175670266 28.371348539415763 28.7515006002401 23.02921168467387 +33 22.084416883376676 28.62572514502901 27.265453090618124 22.024404880976196 +34 21.304260852170433 29.285857171434287 27.265453090618124 22.144428885777156 +35 21.084216843368676 29.425885177035404 28.225645129025807 21.264252850570113 +36 21.404280856171233 28.665733146629325 28.805761152230446 21.124224844968996 +37 20.888355342136855 29.43177270908363 28.53141256502601 21.1484593837535 +38 21.864372874574915 29.02580516103221 28.065613122624526 21.044208841768352 +39 21.928771508603443 28.251300520208083 27.050820328131252 22.769107643057225 +40 21.32 28.499999999999996 27.82 22.36 +41 21.673003802281368 28.116870122073244 27.61656994196518 22.59355613368021 +42 22.29337602561537 28.036822093255953 27.71662997798679 21.953171903141886 +43 21.244248849769953 28.425685137027408 27.825565113022606 22.504500900180034 +44 21.404280856171233 28.985797159431886 27.545509101820365 22.064412882576516 +45 21.86 29.28 26.740000000000002 22.12 +46 21.26 27.54 27.74 23.46 +47 21.628651460584233 28.271308523409367 28.051220488195277 22.048819527811126 +48 21.279999999999998 28.439999999999998 26.400000000000002 23.880000000000003 +49 21.044208841768352 29.045809161832363 27.185437087417487 22.724544908981798 +50 21.82873149259704 28.15126050420168 26.810724289715886 23.209283713485394 +51 21.028411364545818 27.73109243697479 28.011204481792717 23.229291716686674 +52 21.824364872974595 30.146029205841167 25.94518903780756 22.084416883376676 +53 21.164232846569313 28.325665133026607 28.045609121824366 22.464492898579717 +54 21.53907976692787 27.024311834438418 26.90375728350412 24.5328511151296 >>END_MODULE >>Per sequence GC content warn #GC Content Count -0 15.0 -1 15.5 -2 16.5 -3 17.0 -4 18.0 -5 21.5 -6 26.5 -7 30.0 -8 33.5 -9 36.0 -10 41.0 -11 47.0 -12 47.5 -13 56.0 -14 65.5 -15 69.0 -16 72.5 -17 78.0 -18 86.5 -19 94.5 -20 105.5 -21 113.0 -22 123.0 -23 132.5 -24 149.5 -25 172.5 -26 200.0 -27 222.0 -28 247.0 -29 282.0 -30 310.5 -31 337.0 -32 370.0 -33 403.5 -34 438.0 -35 465.0 -36 481.5 -37 511.0 -38 523.5 -39 506.0 -40 491.0 -41 491.5 -42 482.0 -43 483.0 -44 487.5 -45 471.5 -46 471.0 -47 468.5 -48 473.5 -49 472.0 -50 437.5 -51 415.5 -52 407.5 -53 398.0 -54 381.0 -55 364.0 -56 349.0 -57 341.0 -58 330.5 -59 320.0 -60 320.5 -61 301.0 -62 275.0 -63 245.0 -64 207.0 -65 190.5 -66 182.0 -67 173.0 -68 167.0 -69 151.5 -70 131.5 -71 121.0 -72 117.5 -73 110.5 -74 104.0 -75 90.5 -76 75.0 -77 67.5 -78 62.5 -79 61.5 -80 59.5 -81 57.0 -82 54.5 -83 47.0 -84 39.5 -85 38.0 -86 36.5 -87 35.5 -88 28.0 -89 21.0 -90 19.5 -91 17.0 -92 15.5 -93 14.5 -94 13.5 -95 13.5 -96 15.0 -97 15.5 -98 17.0 -99 16.0 -100 13.5 +0 3.0 +1 5.0 +2 7.0 +3 7.0 +4 7.0 +5 5.0 +6 2.0 +7 1.0 +8 1.5 +9 2.0 +10 1.5 +11 1.0 +12 2.5 +13 4.0 +14 4.0 +15 4.0 +16 5.0 +17 6.0 +18 11.0 +19 19.5 +20 23.0 +21 37.5 +22 52.0 +23 61.0 +24 70.0 +25 79.0 +26 88.0 +27 117.5 +28 147.0 +29 159.5 +30 172.0 +31 203.0 +32 243.0 +33 252.0 +34 290.0 +35 328.0 +36 330.0 +37 332.0 +38 332.5 +39 333.0 +40 342.0 +41 351.0 +42 343.5 +43 336.0 +44 318.5 +45 279.5 +46 258.0 +47 254.5 +48 251.0 +49 234.5 +50 218.0 +51 209.5 +52 201.0 +53 198.5 +54 196.0 +55 177.0 +56 162.5 +57 167.0 +58 147.0 +59 127.0 +60 106.5 +61 86.0 +62 85.0 +63 84.0 +64 61.5 +65 39.0 +66 42.5 +67 46.0 +68 34.0 +69 17.0 +70 12.0 +71 14.0 +72 16.0 +73 12.5 +74 9.0 +75 6.5 +76 4.0 +77 4.5 +78 5.0 +79 5.0 +80 5.0 +81 3.0 +82 1.0 +83 1.0 +84 1.0 +85 1.0 +86 1.0 +87 1.0 +88 1.0 +89 1.0 +90 2.0 +91 3.0 +92 2.5 +93 2.0 +94 2.0 +95 3.0 +96 4.0 +97 5.0 +98 6.0 +99 3.5 +100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count @@ -266,95 +810,69 @@ 7 0.0 8 0.0 9 0.0 -10-11 0.0 -12-13 0.0 -14-15 0.0 -16-17 0.0 -18-19 0.0 -20-21 0.0 -22-23 0.0 -24-25 0.0 -26-27 0.0 -28-29 0.0 -30-31 0.0 -32-33 0.0 -34-35 0.0 -36-37 0.0 -38-39 0.0 -40-41 0.0 -42-43 0.0 -44-45 0.0 -46-47 0.0 -48-49 0.0 -50-51 0.0 -52-53 0.0 -54-55 0.0 -56-57 0.0 -58-59 0.0 -60-61 0.0 -62-63 0.0 -64-65 0.0 -66-67 0.0 -68-69 0.0 -70-71 0.0 -72-73 0.0 -74-75 0.0 -76-77 0.0 -78-79 0.0 -80-81 0.0 -82-83 0.0 -84-85 0.0 -86-87 0.0 -88-89 0.0 -90-91 0.0 -92-93 0.0 -94-95 0.0 -96-97 0.0 -98-99 0.0 -100-101 0.0 -102-103 0.0 -104-105 0.0 -106-107 0.0 -108 0.0 +10 0.0 +11 0.0 +12 0.0 +13 0.0 +14 0.0 +15 0.0 +16 0.0 +17 0.0 +18 0.0 +19 0.0 +20 0.0 +21 0.0 +22 0.0 +23 0.0 +24 0.0 +25 0.0 +26 0.04 +27 0.02 +28 0.0 +29 0.02 +30 0.0 +31 0.0 +32 0.04 +33 0.02 +34 0.02 +35 0.02 +36 0.02 +37 0.04 +38 0.02 +39 0.04 +40 0.0 +41 0.06 +42 0.06 +43 0.02 +44 0.02 +45 0.0 +46 0.0 +47 0.04 +48 0.0 +49 0.02 +50 0.04 +51 0.04 +52 0.02 +53 0.02 +54 0.45999999999999996 >>END_MODULE ->>Sequence Length Distribution warn +>>Sequence Length Distribution pass #Length Count -0-4 98.0 -5-9 250.0 -10-14 257.0 -15-19 279.0 -20-24 321.0 -25-29 396.0 -30-34 401.0 -35-39 433.0 -40-44 401.0 -45-49 385.0 -50-54 320.0 -55-59 5.0 -60-64 1.0 -65-69 0.0 -70-74 0.0 -75-79 0.0 -80-84 0.0 -85-89 0.0 -90-94 0.0 -95-99 33.0 -100-104 0.0 -105-109 1325.0 +54 5000.0 >>END_MODULE >>Sequence Duplication Levels pass -#Total Deduplicated Percentage 98.73598369011212 +#Total Deduplicated Percentage 99.52 #Duplication Level Percentage of deduplicated Percentage of total -1 99.44249432170142 98.1855249745158 -2 0.47491224447656405 0.9378185524974516 -3 0.041296716911005574 0.12232415902140673 -4 0.020648358455502787 0.08154943934760449 -5 0.0 0.0 +1 99.81913183279742 99.33999999999999 +2 0.08038585209003216 0.16 +3 0.04019292604501608 0.12 +4 0.0 0.0 +5 0.02009646302250804 0.1 6 0.0 0.0 -7 0.0 0.0 +7 0.04019292604501608 0.27999999999999997 8 0.0 0.0 9 0.0 0.0 ->10 0.020648358455502787 0.672782874617737 +>10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 @@ -364,61 +882,52 @@ >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source -ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672782874617737 No Hit +CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT 7 0.13999999999999999 Illumina Paired End PCR Primer 2 (96% over 33bp) +CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC 7 0.13999999999999999 Illumina Paired End PCR Primer 2 (100% over 35bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 -2 0.0 0.0 0.0 0.0 0.0 -3 0.0 0.0 0.0 0.0 0.0 -4 0.0 0.0 0.0 0.0 0.0 -5 0.0 0.0 0.0 0.0 0.0 -6 0.0 0.0 0.0 0.0 0.0 -7 0.0 0.0 0.0 0.0 0.0 -8 0.0 0.0 0.0 0.0 0.0 -9 0.0 0.0 0.0 0.0 0.0 -10-11 0.0 0.0 0.0 0.0 0.0 -12-13 0.0 0.0 0.0 0.0 0.0 -14-15 0.0 0.0 0.0 0.0 0.0 -16-17 0.0 0.0 0.0 0.0 0.0 -18-19 0.0 0.0 0.0 0.0 0.0 -20-21 0.12232415902140673 0.0 0.0 0.0 0.0 -22-23 0.12232415902140673 0.0 0.0 0.0 0.0 -24-25 0.12232415902140673 0.0 0.0 0.0 0.0 -26-27 0.1325178389398573 0.0 0.0 0.0 0.0 -28-29 0.2038735983690112 0.0 0.0 0.0 0.0 -30-31 0.22426095820591233 0.0 0.0 0.0 0.0 -32-33 0.22426095820591233 0.0 0.0 0.0 0.0 -34-35 0.24464831804281345 0.0 0.0 0.0 0.0 -36-37 0.3058103975535168 0.0 0.0 0.0 0.0 -38-39 0.4383282364933741 0.0 0.0 0.0 0.0 -40-41 0.4689092762487258 0.0 0.0 0.0 0.0 -42-43 0.4689092762487258 0.0 0.0 0.0 0.0 -44-45 0.4689092762487258 0.0 0.0 0.0 0.0 -46-47 0.4689092762487258 0.0 0.0 0.0 0.0 -48-49 0.4689092762487258 0.0 0.0 0.0 0.0 -50-51 0.4689092762487258 0.0 0.0 0.0 0.0 -52-53 0.4689092762487258 0.0 0.0 0.0 0.0 -54-55 0.4689092762487258 0.0 0.0 0.0 0.0 -56-57 0.4689092762487258 0.0 0.0 0.0 0.0 -58-59 0.4689092762487258 0.0 0.0 0.0 0.0 -60-61 0.4689092762487258 0.0 0.0 0.0 0.0 -62-63 0.4689092762487258 0.0 0.0 0.0 0.0 -64-65 0.4689092762487258 0.0 0.0 0.0 0.0 -66-67 0.4689092762487258 0.0 0.0 0.0 0.0 -68-69 0.4689092762487258 0.0 0.0 0.0 0.0 -70-71 0.4689092762487258 0.0 0.0 0.0 0.0 -72-73 0.4689092762487258 0.0 0.0 0.0 0.0 -74-75 0.4689092762487258 0.0 0.0 0.0 0.0 -76-77 0.4689092762487258 0.0 0.0 0.0 0.0 -78-79 0.4689092762487258 0.0 0.0 0.0 0.0 -80-81 0.4689092762487258 0.0 0.0 0.0 0.0 -82-83 0.4689092762487258 0.0 0.0 0.0 0.0 -84-85 0.4689092762487258 0.0 0.0 0.0 0.0 -86-87 0.4689092762487258 0.0 0.0 0.0 0.0 -88-89 0.4689092762487258 0.0 0.0 0.0 0.0 -90-91 0.4689092762487258 0.0 0.0 0.0 0.0 -92-93 0.4689092762487258 0.0 0.0 0.0 0.0 -94-95 0.4689092762487258 0.0 0.0 0.0 0.0 -96-97 0.47910295616717635 0.0 0.0 0.0 0.0 +2 0.02 0.0 0.0 0.0 0.0 +3 0.02 0.0 0.0 0.0 0.0 +4 0.02 0.0 0.0 0.0 0.0 +5 0.02 0.0 0.0 0.0 0.0 +6 0.02 0.0 0.0 0.0 0.0 +7 0.02 0.0 0.0 0.0 0.0 +8 0.02 0.0 0.0 0.0 0.0 +9 0.02 0.0 0.0 0.0 0.0 +10 0.02 0.0 0.0 0.0 0.0 +11 0.02 0.0 0.0 0.0 0.0 +12 0.02 0.0 0.0 0.0 0.0 +13 0.02 0.0 0.0 0.0 0.0 +14 0.02 0.0 0.0 0.0 0.0 +15 0.02 0.0 0.0 0.0 0.0 +16 0.02 0.0 0.0 0.0 0.0 +17 0.02 0.0 0.0 0.0 0.0 +18 0.02 0.0 0.0 0.0 0.0 +19 0.06 0.0 0.0 0.0 0.0 +20 0.36 0.0 0.0 0.0 0.0 +21 0.36 0.0 0.0 0.0 0.0 +22 0.36 0.0 0.0 0.0 0.0 +23 0.36 0.0 0.0 0.0 0.0 +24 0.36 0.0 0.0 0.0 0.0 +25 0.4 0.0 0.0 0.0 0.0 +26 0.4 0.0 0.0 0.0 0.0 +27 0.42 0.0 0.0 0.0 0.0 +28 0.62 0.0 0.0 0.0 0.0 +29 0.66 0.0 0.0 0.0 0.0 +30 0.68 0.0 0.0 0.0 0.0 +31 0.68 0.0 0.0 0.0 0.0 +32 0.68 0.0 0.0 0.0 0.0 +33 0.68 0.0 0.0 0.0 0.0 +34 0.7 0.0 0.0 0.0 0.0 +35 0.76 0.0 0.0 0.0 0.0 +36 0.8 0.0 0.0 0.0 0.0 +37 0.88 0.0 0.0 0.0 0.0 +38 1.0 0.0 0.0 0.0 0.0 +39 1.12 0.0 0.0 0.0 0.0 +40 1.22 0.0 0.0 0.0 0.0 +41 1.38 0.0 0.0 0.0 0.0 +42 1.48 0.0 0.0 0.0 0.0 +43 1.68 0.0 0.0 0.0 0.0 >>END_MODULE